LOCUS       DATBSW010000001         6876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_670084, whole genome shotgun sequence.
ACCESSION   DATBSW010000001 DATBSW010000000
VERSION     DATBSW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670084"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VIF63_00005"
     CDS             <1..573
                     /locus_tag="VIF63_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237798.1"
                     /translation="VSAEPRDATISYPGGLRARWRWGGSGQAAAIFALSEVGGQLVDL
                     GREVAAAPDELCRAELRVDGPAGGWSVRLASTINDEPAAQLWDTAGQLVVKYGFHGYG
                     LDARTGACQWMHRSATPLLAVFASSRLRHVLFQAELETFAIEADGTVAWRIAHTDVVT
                     EAALVGGRLVLTAYGGGVNAIDPATGRGDG"
     gene            835..1779
                     /locus_tag="VIF63_00010"
     CDS             835..1779
                     /locus_tag="VIF63_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237799.1"
                     /translation="MAPLLLAAAGVVALAIAVAILRSFGPGFRVGRLLAAVPQVSVAE
                     AIRIAEAGERRYVRIDGRIDSDADFEDADHRPLVLRRTTLAWRPQGSRGGWKTAETRL
                     EVVRFVVSEGLDEIDVDGSSLDEGLVTVPRESVGRADDVDQLTAAGIPADAQARLRVG
                     HVSTIDHATVLGVPSKGAGGVVMIGPGLGRPLILSTLERDEAMRVLSGGAINRSRAAI
                     ACLAAGGALLALALVWWLVDAMTGGVATVLAASPDPTLRPGTDTRTSGGGPGIVGEPL
                     LALFGVLGIALVSLGASLAYVRLTRDPKVLPGNRRPRP"
     gene            1890..4754
                     /locus_tag="VIF63_00015"
     CDS             1890..4754
                     /locus_tag="VIF63_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8237800.1"
                     /translation="MDLLHPRPLDATLSVTRAAEVLGVHANTVRAWSDAGRLRYYRIN
                     PRGDRRYRNGDLQRFLAALDSTGHAPDPESARSGGNGRRGPVVGLDEHLTMLNLIGEL
                     SALSGSAIRDALTNPMAPLVAAVHAIHDALGVVHASAWRREGTRLVPVAVSGAPGVGV
                     VPVPTSFGVLGEALADPGSVIEAEPDDRLSATNLSGREVACAIPGDADSRGALLLVRP
                     TPESLTDSERELIRVATTALGNIIEATASAVIVSHKLQRADALRRVTSDIGSRLDLDE
                     ILDRVVDHAQVLFSADRAAAFLFEEDGQRRMAASRGLSQAWINAVVSVEGTTLGTHAI
                     VARRPMYSERYADDPRAGNLRAAVVQEGFDTVCVAPLLDGDKPEPLGILGVYHDQPHP
                     WSEDELDTMAALATQVTVAIKAANNYAQLATWAAQLQSIQALGARLNRLTRVQEIGNA
                     IATELRQLIDYHNVRVYRLTGDDLIPVAMQGRVGEYLDETPDQLKVKFGQGITGWVAQ
                     HRIAQRLDNAATDPRAKTIAGTEDDMDESMLLAPMLFEDDVLGVLVLSKLGLRQFRGD
                     DLRLLEIYASFAAQAMANADATERLREKSAALEQKVRGQRELLEITESIVQTYDSEVL
                     LGTIADRLGELIGSDNVAIELVDQTVGLLSPVIARGVDAEYYGLPWQAGETGLATWVL
                     DHNEPVRVEDEFTDPRIAQPPSGPVHGSLVCVPLRGRDGAIGVLTLERIGEGRVFSDD
                     EFELVQLFAAQASIALQNAEVHLDVRRRAQTDVLTGLLNHGTFAQQLDGLIAAGEPFS
                     LVMLDLDRFKPVNDGMGHQAGDLLLRHVAEAIVAASRDSDRVYRYGGDEFAVILPRTD
                     GDHVAPIAERVRAAVKSVVGPGTTWRGKARSLEASAGTASFPTDGTRPEEVLLAADRA
                     LFVAKRSGGGRVASAAEGEALAGEFSLQTPTPIDPLATPA"
     gene            4848..5312
                     /locus_tag="VIF63_00020"
     CDS             4848..5312
                     /locus_tag="VIF63_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8237801.1"
                     /translation="MTARSPRRADRSVLDSPWLAAAIVAIVLGVGAAILLPGFLGPSG
                     PGSTPGPGATSLAPGPSPTFVGPTFVRPTPSPQPGFTTYVVKRGDTLGSIATEFHTTA
                     RSISWWSRGRYPSLDPESDNYNPNNIKLGWELLLIPGAVVDENNPPPASPKP"
     gene            complement(5337..6101)
                     /locus_tag="VIF63_00025"
     CDS             complement(5337..6101)
                     /locus_tag="VIF63_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8237802.1"
                     /translation="MSVTAAAPPALRITGLRKAFGDLVVLDGIDLEVAEHEVICLIGA
                     SGSGKSTLLRCINLLEPIQAGRIEVGGEEITAPGVSVDRVRRGIGIVFQAFNLFPHLR
                     VLDNVTLAPRRVLGQSKAEAEAAGTELLRRFGLDDKRSEYPDRLSGGQQQRVAIVRAL
                     AMQPRLMLLDEVTSALDPELIAEVLDVVRELARAGMTMVIATHEMGFARDVADRICFL
                     DGGRILEQGPPAQLLTAPTEARTQQFLQRVIAAGRL"
     gene            complement(6098..>6876)
                     /locus_tag="VIF63_00030"
     CDS             complement(6098..>6876)
                     /locus_tag="VIF63_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8237803.1"
                     /translation="SWIAFASTLATFGLLGWVVVNAPNWAAVQRSFLSGEEFTDALPA
                     VVWALRINVQLFLFAEVLILIVGLGIAVLRSLPGAVFFPVRLLAIAYVDIFRALPGVL
                     VIFFLGLGVPSLRLPGVPKEPFFWGVIALTLIYSAYVSEVYRAGIESIHPSQDAAARS
                     LGLTRAQSMRHVILPQAIRRVIPPLLNDFIGLQKDTVLVSFIGVIEVFRTAQIRQSAH
                     FNFTPYLAVALVYLLVTLPLTRVTDWLITRDRLRRAGGRV"
BASE COUNT          997 a         2492 c         2399 g          988 t
ORIGIN      
        1 gtgagcgccg agccgcgcga cgcgacgatc agctatcccg gcgggctgcg ggcgcgctgg
       61 cgctggggcg gctccggcca ggcggcggcg atcttcgcgc tctcggaggt cggcgggcag
      121 ctggtcgatc tcgggcggga ggtcgcggcc gcgcccgacg agctgtgtcg ggcggagctg
      181 cgcgtcgacg gccctgccgg aggctggagc gtccgcctcg cctcgacgat caacgacgag
      241 cccgccgcgc agctgtggga cacggccggc cagctggtcg tgaagtacgg cttccatggt
      301 tacgggctcg acgcccgaac cggcgcgtgc cagtggatgc atcgatcggc gacgccgctg
      361 ctcgcggtgt tcgcgtcctc gcgcctgcgc cacgtcctgt tccaggcgga actggagaca
      421 ttcgcgatcg aggccgacgg cacggtcgcg tggcggatcg cgcacaccga tgtggtcacc
      481 gaggccgcgc tggtcggcgg gcggctcgtg ttgacggcct acggcggcgg cgtgaacgcc
      541 atcgatccgg cgacggggcg cggcgacggc tgacccgcgg cgccatgcgc gagcgcggcc
      601 gcgatgtgga cccgtcgtgg acaagccagc cgatcagcgc gaatctcgtg gataggtgcc
      661 attgaccggc tacccctgcg gccctagcgt agttctcagc ccggaggggc cgagcgacac
      721 taacgactga gatcgcatca aggtccacgt gttgggtagg ttcctccggg ccctttgatt
      781 cccccggcct ggccccgacg ccggctcgcg agcccgttgc ctatgatccc gggcgtggct
      841 cccctcctcc ttgcggccgc aggcgtcgtc gccctggcca tcgccgtcgc gatcctgcgc
      901 agcttcggtc ccggctttcg cgtgggccgc ctgctggctg ccgtgcccca ggtcagcgtc
      961 gccgaggcga tccggatcgc cgaggcgggc gagcgccgtt acgtgcgcat cgacgggcgg
     1021 atcgacagcg acgcggactt cgaggatgcg gatcaccggc ccctcgtgct ccgacgcacg
     1081 accctcgcct ggcgtcctca gggatcgcgc ggcggctgga agactgcgga gacccgcctc
     1141 gaggtagtcc gcttcgtcgt cagcgagggc cttgacgaga tcgacgtcga tggctcgtcg
     1201 ctcgacgaag gcctcgtgac ggtgccgcgc gagagcgtcg gccgcgccga cgatgtcgac
     1261 cagctcacgg cggccggcat tcccgccgat gcccaggcgc ggctgagggt cggccacgtc
     1321 tcgaccatcg atcacgccac ggtcctgggc gtcccgagca agggcgccgg cggcgtggtc
     1381 atgatcgggc ctgggctggg acgtcccctg atcctgtcga cgctcgagcg cgacgaggcc
     1441 atgcgcgtgc tcagtggcgg cgcgatcaac cgatcgcgcg cggcgatcgc gtgtctcgcc
     1501 gccggcggag ccctgcttgc cctcgcgttg gtctggtggc tggtggacgc gatgaccggc
     1561 ggcgtggcga cggtcctcgc cgcctcgccc gaccccacgc ttcgacccgg caccgacacg
     1621 cgcaccagtg gcggcgggcc ggggatcgtc ggcgagccac tcctcgcgct gtttggcgtg
     1681 ctcggcatcg cgctcgtgag tctcggcgcg agcctcgcct acgtgcgcct cacgagggac
     1741 ccgaaagtcc tacctgggaa tcgccgcccg cggccctgac cggtgcatcc gggcactcgg
     1801 tactttccga ccattggcaa gaaatgtggg gtcggtaggc tgctgccata acttcacgaa
     1861 tcttcccggg ggcccaggac ttgccaaagg tggatctgct ccatccgaga ccgctcgacg
     1921 cgacgctgag cgtcacgcgc gcggccgagg tgctgggcgt gcacgcgaac accgtgcgcg
     1981 cctggagcga tgccggccgc ctgcgctact accgcatcaa cccgcggggc gatcggcgct
     2041 accgcaacgg cgacctgcaa cggttcctcg ccgccctcga ctcgaccggc cacgcgcccg
     2101 accccgagag cgcccgcagc ggaggcaacg gccgccgcgg cccggtcgtc gggctcgacg
     2161 agcacctgac catgctcaac ctcatcggcg agctgtccgc gctgagcggc tcggccatcc
     2221 gcgatgccct gaccaacccc atggcgccgc tcgtcgccgc ggtgcacgca atccatgatg
     2281 cgctcggggt cgtgcatgcc tcggcctggc gccgcgaggg cactcggctg gtgccggtgg
     2341 ccgtgtccgg tgcgccgggc gtcggcgtcg tcccggtgcc cacctcgttc ggtgtgctcg
     2401 gcgaagcgct ggccgatccg ggttcagtga tcgaggccga gccggacgat cggctttcgg
     2461 caacgaacct gagcggtcgc gaggtcgcct gcgcgatccc cggcgacgcg gactcgcggg
     2521 gcgcgctcct cctggtccgg ccgacgcccg agtccctgac ggattcggag cgcgagctca
     2581 ttcgcgtcgc caccaccgcc ctcggcaaca tcatcgaggc gaccgcctcg gcggtcatcg
     2641 tctcgcacaa gctccagcgc gccgacgcgc tccggcgggt cacgagcgac atcggcagca
     2701 ggctcgacct cgacgagatc ctcgatcgcg tcgtggacca cgcgcaggtg ctgttctccg
     2761 ccgaccgcgc cgccgcgttc ctgttcgagg aggacggcca gcgccggatg gccgcgtcgc
     2821 gtggcctgtc acaggcatgg atcaacgccg tcgtctccgt cgagggcacg accctcggca
     2881 cccacgcgat cgttgcccgc cgcccgatgt actccgagcg ctacgccgac gacccgcgcg
     2941 ccggcaacct tcgggccgcc gtggtccagg agggcttcga taccgtctgc gtcgcgccgc
     3001 tcctcgatgg cgacaagccg gagccgctgg gcatccttgg cgtctaccac gaccagccgc
     3061 atccctggtc cgaggacgag ctcgacacca tggccgccct cgccacccag gtgaccgtcg
     3121 cgatcaaggc ggcgaacaac tacgcacagc tcgcgacctg ggcggcgcag ctccagtcga
     3181 tccaggccct cggtgcgcgc ctcaaccgcc tgacccgcgt ccaggagatc ggcaacgcaa
     3241 tcgcgaccga gctccgccag ctgatcgact atcacaacgt gcgcgtctat cgcctgaccg
     3301 gcgacgacct catcccggtc gcgatgcagg gtcgcgtcgg cgagtacctc gatgagacgc
     3361 ccgaccagct caaggtcaag ttcggccagg gcatcacggg ctgggtcgcc cagcaccgga
     3421 ttgcccagcg cctcgacaac gccgcgaccg atccccgtgc gaaaacgatc gccggcaccg
     3481 aggacgacat ggacgagtcg atgctcctcg cgccgatgct gttcgaagac gacgtcctcg
     3541 gcgtgctcgt gctctcgaag ctcgggcttc gccagttccg cggtgacgac ctgcgcctgc
     3601 tcgagatcta tgcgagcttc gccgcgcagg cgatggccaa cgccgacgcg accgagcgcc
     3661 tgcgcgagaa gtcggcggcg ctcgagcaga aggtccgcgg ccagcgcgag ctgctcgaga
     3721 tcacggaatc gatcgtgcag acgtacgact cggaggtcct gctcggcacc atcgccgacc
     3781 gcctcggcga gctgatcggc tcggacaacg tcgcgatcga gctcgtcgac cagacggtag
     3841 gcctcttgtc gccggtcatc gcccggggcg tcgatgccga gtactacggc ctgccctggc
     3901 aagctggcga gacaggcctc gcgacgtggg tgctggatca caacgagccc gtccgcgtcg
     3961 aggatgagtt caccgatccg cgtatcgccc agccgccgag cgggccggtc cacggcagcc
     4021 tcgtgtgcgt gccgctccgt ggtcgggacg gcgcgatcgg cgtgctgacg ctcgagcgca
     4081 tcggcgaggg ccgcgtgttc agcgatgacg agttcgagct cgtgcagctg ttcgccgccc
     4141 aggcctcgat cgccctccag aacgccgagg tccacctcga cgtccggcgc cgcgcccaga
     4201 ccgacgtcct caccgggctg ctgaatcacg gcacgttcgc ccagcagctc gacgggctca
     4261 tcgccgccgg cgagccgttc agcctcgtga tgctcgacct cgaccggttc aagccggtca
     4321 acgacggcat gggccaccag gccggcgacc tcctcctgcg ccacgtcgcc gaggccatcg
     4381 tggccgcgag tcgcgacagc gatcgtgtct accgctacgg cggcgacgag ttcgccgtga
     4441 tcctgccgcg caccgacggc gaccatgtcg caccgatcgc cgagcgcgtg cgcgccgcgg
     4501 tcaagagcgt tgtcgggccc ggcaccacgt ggcgcggcaa ggcacgctcg ctcgaggcct
     4561 cggccgggac cgcgtcgttc ccgacggacg gcacgcggcc cgaggaagtc ctcctcgccg
     4621 ccgaccgcgc gctgttcgtc gccaagcggt ccggaggcgg ccgggttgcg agcgccgccg
     4681 agggcgaggc cctcgccggt gagttctcgc tccagacgcc gaccccgatc gatccgctgg
     4741 ccacgcccgc ctgacggttc gtcgctggtg ggatttacga gctcgtcttc gccgcagtgt
     4801 caccgccgcc gtcgcggagc gggaccctcg gccgtcagac tcgggtcatg accgccagat
     4861 ccccgcgcag ggctgatcgt tccgttctcg actcgccgtg gctcgctgcg gcgatcgtcg
     4921 cgatcgtgct cggcgtcggc gccgcgatcc tgctgccggg gttcctcggg ccttcgggtc
     4981 ccgggtcgac gcctggtccg ggcgccacct cgctcgcccc gggcccgagc cccacgttcg
     5041 tcggcccgac cttcgtccgc ccgacaccga gcccgcagcc cgggttcacg acctacgtgg
     5101 tgaagcgtgg cgacaccctc ggctcgatcg ccaccgagtt ccatacgacc gcccgctcga
     5161 tctcctggtg gagccgcggc cgttatccgt cactcgaccc cgaatccgac aactacaacc
     5221 cgaacaacat caagctcggc tgggagctcc tgctgatccc gggcgcggtc gtcgacgaga
     5281 acaacccacc accggcaagc ccgaagccgt agcccttgcc ctcgccctgg ccgggcctac
     5341 aaccggccgg cggcgatcac ccgctgcagg aactgctggg tccgggcttc tgtcggggcg
     5401 gtgagcagct gggcgggcgg gccctgctcg aggatccggc cgccgtcgag gaagcagatg
     5461 cgatcggcga cgtcgcgggc gaagcccatc tcgtgcgtcg cgatgaccat cgtcatgccc
     5521 gcgcgggcga gctcgcggac gacatcgagg acctcggcga tcagctccgg gtcgagggcg
     5581 ctcgtcacct cgtcgaggag catgagccgc ggctgcatcg cgagcgcgcg cacgatcgcg
     5641 acgcgctgct gctggccgcc ggacagccgg tccgggtact cggatcgctt gtcgtcgagg
     5701 ccgaagcggc gcaggagctc cgtgccggcc gcctccgcct cggccttcga ctggccgagc
     5761 acccggcgcg gcgcgagggt cacgttgtcg aggacgcgca ggtgcgggaa caggttgaac
     5821 gcctggaaca cgatgccgat gccccgccga acgcggtcga cgctcacccc gggcgcggtg
     5881 atctcctcgc caccgacctc gatccgcccg gcctggatcg gctcgagcag gttgatgcag
     5941 cgcagcaggg tcgacttgcc ggagcccgag gcgccgatca ggcagatgac ctcgtgctcg
     6001 gcgacctcga ggtcgatccc gtcgagcacg acgaggtcgc cgaaggcctt gcgcaggccc
     6061 gtgatccgga gtgcgggcgg tgccgcggca gtcacgctca tacgcggccg ccggcgcgcc
     6121 ggaggcggtc gcgggtgatg agccagtccg tgacccgcgt gagcggcagc gtcaccagca
     6181 ggtacacgag cgcgaccgcg aggtacggcg tgaagttgaa gtgggccgac tgccggatct
     6241 gggcggtccg gaagacctcg atcaccccga tgaacgacac gagcaccgtg tccttctgga
     6301 gtccgatgaa gtcgttgagc agcggcggga tcacgcgccg gatcgcctgc ggcaggatca
     6361 cgtggcgcat cgactgggcg cgcgtcaggc caagcgagcg cgcggcggcg tcctggctgg
     6421 gatggatcga ctcgatgccg gcccggtaga cctcggacac gtatgccgag tagatcaacg
     6481 tcagggcgat gacgccccag aagaagggct ccttcggaac cccggggagt cgcaggctcg
     6541 gcacgccgag acccaggaag aagatcacga ggaccccggg cagggcgcgg aagatgtcca
     6601 cgtaggcgat cgccagcagc cggaccggga agaagacggc gccgggcagg ctgcgcagga
     6661 ccgcgatccc gagtcccacg atcaggatca gaacctcggc gaacaggaac agctggacgt
     6721 tgatgcggag cgcccagacg accgcgggca gcgcgtcggt gaactcctcg ccgctcagga
     6781 acgagcgctg gaccgccgcc cagttcgggg cgttgacgac cacccagccg agcaggccga
     6841 aggtcgccag cgtgctggcg aaggcgatcc agcttg
//
LOCUS       DATBSW010000002        10839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_70553, whole genome shotgun sequence.
ACCESSION   DATBSW010000002 DATBSW010000000
VERSION     DATBSW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10839
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70553"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /locus_tag="VIF63_00035"
     CDS             <1..1966
                     /locus_tag="VIF63_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8237804.1"
                     /translation="SKATKATAAATAEAAEAAATLEAATLEAATVEAATLEPGTLEAS
                     EASEASAATEVAEATAEATPATAEAPLGTFVPALIPVQPDKPAGPRNVAREEMLHGIR
                     LRMQREVINAFDTLLDGTTVDTKTKIEGIVDRVIQANGFAVNRDERLRLIEEMVHEIT
                     GFGPLEPLLNDPTITEVMVNGPDHIYIERAGKIQRVDSFFLNEEHVLRIIDRIITPLG
                     RRIDETSPRVDARLPDGSRVNAIIAPLSLIGPVITVRKFSKTPYTVQNLINFGTSTPE
                     MFDFLRSCIEARLNVFVSGGTGSGKTTFLNVLSSFIPNDERIVTIEDAAELQLRQEHV
                     VTLEARPANIEGMGEISIRDLLRNAMHMRPDRIIVGECRSGEALDMLQAMTTGQDGSL
                     STGHANTPRDMLRRLETMVLMTGYELPLRAIREQIASAVDVLVHTARLKDGSRKVTNI
                     TEVYGIEDDEILTQDIFTFVQTGVVDGKIQGEPKATGIRPTFMGQFARAGIELPPGEY
                     GIPPEDPAKPIRPQKGRFGLKDASETADVSVQKLGEGRAVKAGGMLYVTSVGPVNPET
                     QRVIRGGIKEHSRQALANLKAMLEANGSSLEKIVWANWSLREPSDFEIFNEEWVKVFP
                     ADGPIGQSTLMPPLHRRAGFRVTLGVIAEA"
     gene            complement(1986..3218)
                     /locus_tag="VIF63_00040"
     CDS             complement(1986..3218)
                     /locus_tag="VIF63_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237805.1"
                     /translation="MTGTGDGRGGGQRGGARPGRDRERGGDRYRAKPGTEPFDSTGWS
                     TGASIEELPTVRLPPTPLGGTRLGPGGMLAVAGTAVLLAAGFGMLGGRAGPSPSPATG
                     AVVPTQPPVTPEPAPTSAPIPQVTPFSDCAPPTTSSTGVQLQVNGQRNAGAIQVIGGI
                     GPLSAYPIPHVIPDVFGSRISVPIDVISELWVVGGACALSWNIGLVDGDVLDAYETTS
                     PDPAVAAQNRFALNLAPYAGTNQVLRADLYFSTLSIRVVWPIHVEAIDRPVGALQDGE
                     GRAAVVEGCDVVVTFGNGAEVPAPATCYADLPIQPTETLTLDRDQPVRFWLGDWQVTN
                     GVLVCGHLSDTYFVTEPTPGCFVDGSTDTDDGSITFDLPPDFEGRWTIGLAACAQSSG
                     VTPNRVCGTWFADVRFRP"
     gene            3230..4030
                     /locus_tag="VIF63_00045"
     CDS             3230..4030
                     /locus_tag="VIF63_00045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY8237806.1"
                     /translation="MQQPTILPIEPSIGRIRLGQRSVEYRVRHSARSRGLRVTIDPRL
                     GLVVSVPPATRRGWGHPEPRIEAFLRERSAWVLRHLDELARERAAAQARGGVRDGGRI
                     PYRGELHRIRVRPAALRGRRSTVERAGAEAGDELVVSMATGDRRPLERILAGWFRDRA
                     SESIDAAIATHAPGLGVRPAKVDLRDPRSRWGSAARTGRLMFSWRLVLAPPASLETVV
                     VHELAHLCAFGHGPAFWALVAARRPSHLADRAWLRRNSHALHAALDPS"
     gene            complement(4038..4122)
                     /locus_tag="VIF63_00050"
     tRNA            complement(4038..4122)
                     /locus_tag="VIF63_00050"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4086..4088),aa:Tyr,seq:gta)
     gene            complement(4171..4581)
                     /locus_tag="VIF63_00055"
     CDS             complement(4171..4581)
                     /locus_tag="VIF63_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8237807.1"
                     /translation="MPERVGGGELLLAVEDEPLNAALLEAILARAGYHLEVATDLAAA
                     REFLAEETPALILLDRHLPDGDGLDLVEPIRAMERLRDVRILLVSASVLPRDQAAALE
                     AGCDGFLAKPVRVKPLVDEVRRLLDDAALAKLAD"
     gene            complement(4636..5655)
                     /locus_tag="VIF63_00060"
     CDS             complement(4636..5655)
                     /locus_tag="VIF63_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8237808.1"
                     /translation="MALGLERELVLVAEDDAANRALLGRLLERNGYRSITVADGRDAV
                     RAASDESPDLVLLDIGLPGLNGLDVCRRLRADPRTVALPIILVTGQTASRDVVAGLDA
                     GADDFVRKPYDEAELMARVRSVLRLARVTAEMVGAHGVIAALANAVEAKDSTTEMHCQ
                     RLAGLAHQLGMSAGLEPAALKGLVFGALLHDIGKIGVSDAILTKPGPLTRQEWAEMRL
                     HPLIGERICEPLATASQFSSIVRHHHERWDGAGYPDGLRGEKIPTGARIVGLVDAYDA
                     IIHDRPYRAARSIDKALTELRTEAGKQFDPALVALFVPMVEHEGAIELRTPAVRALEA
                     LRPAV"
     gene            complement(5747..5822)
                     /locus_tag="VIF63_00065"
     tRNA            complement(5747..5822)
                     /locus_tag="VIF63_00065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5787..5789),aa:Ala,seq:cgc)
     gene            6176..7399
                     /locus_tag="VIF63_00070"
     CDS             6176..7399
                     /locus_tag="VIF63_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237809.1"
                     /translation="MTAPSTDLSTALATLRSRWGAAAPGFVGEVVGALATVPLEDLDQ
                     PGSGPIGVPQQHADPDRIYSTGFAELDAILGPGGVPRGMGVSLRGDASSGRTTLALRL
                     VAEAQAAGSIVAWLDLTAALDPVEAVARGIRPEWLVVLTPADPDEGLAIAGSLLAGRA
                     VDLLVIDLPSKLSKTFADRLGRLAALARRAGITLLVLEPPRATGGLAAAVGEATGIRL
                     ELARRSWIRLGRDVVGQRTEVIVARNRAGPPGRRATLRILYADGGARDACLRRDDLLH
                     DRRLVQPAGVQGAAKPGERTHLECVSEPGAGRNAAIRPDQPAAPIPVPGKRNDATPPS
                     SSPTPPPRLREAAPALRLVATGAGDPRRSAVVERERARRGSDRAGARRSPRDAARVGA
                     PAGAGGDVPRRQARG"
     gene            7284..8408
                     /locus_tag="VIF63_00075"
     CDS             7284..8408
                     /locus_tag="VIF63_00075"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237810.1"
                     /translation="MLDADPTARALGVRRGMPLGSAHRLAPEATFLDAKPEADRAELE
                     AAFERLAGFSPAIAGSTEPTDVAFGLLEAQIDGLEGLWGLEPALVERIGGALEEPLAG
                     RPRAGIAGTRFAATVAALAAEPGSLVSVPPGAEAEFLAPLPASLLTPDADIRARLRRF
                     GLRQVGQVAALATSALVARFGEEGARIGSRSRGLEVDPFRPRRAPERLVLGLPIEPAV
                     AELEAIRFVLRRLAVALATNLAARGLAAEQGRLRLELDLAFARKGTAPELAVETRFPE
                     PTADPEAIERLLLAKLERTPPPAAVARLELELNGASPQAGQQLPLFIPQALHGARLAW
                     QLARLALTYGEDRIHRVEITDPEAPLPEHRWAWRDVSAGT"
     gene            8440..8685
                     /locus_tag="VIF63_00080"
     CDS             8440..8685
                     /locus_tag="VIF63_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237811.1"
                     /translation="MTRLLRGHPSINVEVDDGRVVAIAWAERREPVEVCNRWRVEESW
                     WREPIARDYFKVVGEHWLALIYFDRVAGSWHLERLYD"
     gene            complement(8724..9113)
                     /locus_tag="VIF63_00085"
     CDS             complement(8724..9113)
                     /locus_tag="VIF63_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237812.1"
                     /translation="MSVESAAAISAVGFVVIAVFQAALAAGAPLGRAAWGGRTAVLPA
                     RLRRSSAVAVVIWLVAAAIILGRAGLIDVPLPEVTLTVGAWVLVVLSALGSLVNVASS
                     SPWERFGWAPLAATLAVLSAVVASSPV"
     gene            complement(9148..9654)
                     /locus_tag="VIF63_00090"
     CDS             complement(9148..9654)
                     /locus_tag="VIF63_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237813.1"
                     /translation="MHRGFIALTALLILLAVPVVGGCTPTPTAGSATPTATGPIGTSH
                     GDPTAPPTLGPSLPTQTDTEWGRIWDALPPWFPIPDGASPTETGSGPATAELALPAES
                     TAQAAADFFRTSFQAAGFAAVDVDGPLEDGAFVVSVPGNCQIEVRLAPLGNQVVARVL
                     YGAGCPFE"
     gene            10067..>10839
                     /locus_tag="VIF63_00095"
     CDS             10067..>10839
                     /locus_tag="VIF63_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8237814.1"
                     /translation="MSRSYAELHSHSNFSFLHGASTVEDMTERAAELGLCGLAVTDHD
                     GLYGVVRFSTAAAAAGIRPIIGIEIELVDPAAPDPGGIVVPARRGARGRAPALPGLLA
                     AGADLQPIEGVPARPRPTRARLPGHRAVVKEDLRGIGGRQRGPHLVLLARDATGYRSL
                     CRLVSRANLDGTKRVPRFTHALLERHAEGLLALSGGRESEIARRLQVGDRAGARAVAE
                     RYAALYGSAEFRSRAGTGRGSGGGRGPVAGDSPATAGLF"
BASE COUNT         1603 a         3881 c         3828 g         1527 t
ORIGIN      
        1 gtcaaaggcg acgaaagcca cggcggcggc gacggccgag gcggccgagg cggctgcgac
       61 cctggaggcc gcgaccctgg aggccgcgac ggtggaggcc gcgacgctgg agcccgggac
      121 cctggaggcg tcggaggcgt cggaggcgtc ggcagcgacc gaggtcgcag aggcaacggc
      181 ggaagcaacg cccgcgactg ccgaggcgcc gctgggtacg ttcgttcccg ccctcatccc
      241 cgtgcagccg gacaagcccg ccggtccgcg caatgtcgcc cgcgaggaga tgctccacgg
      301 catccggctg cggatgcagc gcgaggtgat caacgcgttc gacacgctcc tcgacggcac
      361 gacggtcgac accaagacca agatcgaggg catcgtcgac cgggtcatcc aggcgaatgg
      421 cttcgccgtc aaccgcgacg agcgcctgcg cctcatcgag gagatggtcc acgagatcac
      481 cggcttcggc cccctcgagc cgctgctcaa cgacccgacc atcaccgagg tcatggtcaa
      541 cgggccggac cacatctaca tcgagcgcgc cggcaagatc cagcgtgtcg actcgttctt
      601 cctgaacgag gagcacgtcc tccggatcat cgaccgcatc atcaccccgc tcggccgtcg
      661 tatcgacgag accagcccgc gcgttgacgc ccgcctgcca gacggcagcc gcgtgaacgc
      721 gatcatcgcg ccgctgtcgc tgatcgggcc ggtcatcacc gtccgcaagt tctcgaagac
      781 cccgtacacg gtgcagaacc tgatcaactt cggcacctcg acgcccgaga tgttcgactt
      841 cctgcgatcg tgcatcgaag cccgcctgaa cgtgttcgtc tcgggcggca cgggctccgg
      901 caagacgacg ttcctgaacg tgctgtcctc gttcatcccg aacgacgagc ggatcgtcac
      961 gatcgaggac gccgccgagc tccagctccg ccaggaacac gtggtcaccc tcgaggcgcg
     1021 gcccgccaac atcgagggca tgggtgagat ctcgatccgc gacctgctgc gcaacgcgat
     1081 gcacatgcgc cccgaccgga tcatcgtcgg cgagtgccgt tccggcgagg ccctcgacat
     1141 gctccaggcc atgaccaccg gccaggacgg ctcgctctcg accggccacg cgaacacgcc
     1201 gcgggacatg ctccggcgcc tcgagacgat ggtcctcatg accggctacg agctcccgct
     1261 gcgcgcgatc cgcgagcaga tcgcatccgc ggtcgacgtc ctcgtgcaca ccgcccgcct
     1321 caaggacggc tcgcgcaagg tcacgaacat caccgaggtc tacgggatcg aggacgacga
     1381 gatcctgacc caggacatct tcacgttcgt ccagaccggc gtcgtcgacg gcaagatcca
     1441 gggcgagccc aaggcgactg gcatccgccc gacgttcatg ggccagttcg ctcgcgccgg
     1501 catcgagctg ccgccgggcg agtacgggat cccgcccgag gatccggcca agccgatccg
     1561 gccgcagaag ggccggttcg ggctcaagga cgcgtccgag accgccgatg tctccgtgca
     1621 gaagctcggc gaaggtcgcg ccgtgaaggc cggcggcatg ctgtacgtga cgtcggtcgg
     1681 gccggtcaat cccgagacgc aacgcgtcat ccgcggtggg atcaaggagc acagccggca
     1741 ggcgctcgcg aacctcaagg ccatgctcga ggccaacggc agctcgctcg agaagatcgt
     1801 ctgggcgaac tggtccctgc gcgagccgag cgacttcgag atcttcaacg aggagtgggt
     1861 caaggtcttc ccggccgacg gcccgatcgg ccagtcgacc ctgatgccgc cactccaccg
     1921 ccgcgccggc ttccgggtga cgctcggggt catcgccgag gcctgacccg tccagtcgcg
     1981 gatcgctagg gccggaaccg gacgtctgca aaccaggtcc cgcagacccg attcggggtc
     2041 acgccagacg actgtgcaca cgcggcaagc ccgatcgtcc agcgtccctc gaagtccggc
     2101 ggcaggtcga acgtgatcga accgtcgtcg gtgtcggtcg agccgtcaac gaaacagcct
     2161 ggcgtcggct cggtcacgaa gtacgtgtcg gacaggtggc cgcagacgag caccccgttc
     2221 gtcacctgcc agtccccgag ccagaaccgg accggctggt cccggtcgag ggtcagggtc
     2281 tccgtgggtt gaatcggcag gtcggcgtag caggttgccg gtgcagggac ctcagcgccg
     2341 ttgccgaagg tgaccacgac atcgcagccc tcgacaaccg cggcccggcc ttcgccatcc
     2401 tggagtgcgc ccacgggccg gtcgatcgct tcgacatgga tcggccacac gacacggatc
     2461 gatagcgtcg agaagtacag gtcggcgcgc aggacctggt tggtcccggc gtagggcgcc
     2521 aggttcagcg cgaaccggtt ctgtgccgca acggcaggat ccggggacgt cgtttcgtag
     2581 gcatcgagca cgtcaccgtc gaccaggccg atgttccagc tgagggcgca tgcaccgccg
     2641 acgacccaca gctcgctgat cacatcgatg ggcactgaga tcctgctccc gaacacgtca
     2701 ggaatcacat gaggaatcgg ataggccgac agcggaccga tcccgccgat gacctggatg
     2761 gcgcccgcat tccgttgacc attcacctgg agctggactc cggtcgagga ggtcgtcggt
     2821 ggcgcgcaat cgctgaaggg cgtcacctgc ggaatcggtg ccgaggtggg cgccggttct
     2881 ggcgtgaccg gcggctgcgt gggcacgact gcaccggtcg cgggcgacgg gctcgggccg
     2941 gcacgcccgc cgagcatccc gaaccccgcc gccagcagca ccgccgtccc ggcgaccgcg
     3001 agcatcccgc ccgggcctaa ccgggtcccg cccagcggcg tcggcggcag ccggaccgtg
     3061 ggcagctcct cgatcgacgc gccggtggac catcccgtcg aatcgaacgg ctccgtcccg
     3121 ggcttcgcgc gatatcgatc accgcctcgc tcgcgatccc gaccgggacg cgcgccgccg
     3181 cgctggccgc cgccgcggcc gtcaccggtg cctgtcacga tgtgccgaga tgcagcaacc
     3241 gacgatcctc ccgatcgagc cgtccattgg acgcatccgg ctcgggcaac gctccgttga
     3301 gtatcgcgta cgacattcgg cccgctcgcg cggcctgcgc gtcacgatcg accctcgtct
     3361 ggggctggtc gtctcggtgc cgccggcgac ccgtcgcgga tggggccacc cggaaccacg
     3421 gatcgaggcg ttcctgcgcg agcgctcggc gtgggtgctg cgccacctgg acgagctggc
     3481 ccgggagcgt gcagcggccc aggcccgtgg cggcgttcgc gacggtggcc ggatcccgta
     3541 ccgcggcgag ctgcatcgga tccgcgttcg ccccgcagcg ctccggggtc gccgatcgac
     3601 cgtcgaacgg gccggcgccg aggccggcga cgagctcgtg gtgtcgatgg cgacgggcga
     3661 tcgacgccca ctcgagcgca tcctcgccgg ctggttccgg gaccgcgcgt ccgagtcgat
     3721 cgacgccgcg atcgcgacgc atgcaccggg cctgggcgtc cggccagcga aggtcgacct
     3781 gcgcgatccc cgcagccgat gggggagcgc cgcccgcacc ggtcggctga tgttctcgtg
     3841 gcggctcgtg ctcgctccgc ctgcatcgct ggaaacggtc gtggtccacg agctggccca
     3901 cctgtgcgcg ttcggccacg ggccggcgtt ctgggcgctc gtggccgctc ggcgcccgag
     3961 tcatctcgcc gatcgggcct ggctccggcg caactcgcac gccttgcacg ctgccctcga
     4021 cccgtcatag ggcgggatgg tgggccgggg cagaatcgaa ctgccacagt cgaagacgag
     4081 ggttttacag acccttgggc tcaccacctg cccaaccgac ccacgcgctc gctcgaaggg
     4141 ccgacggtgg tgccgggccg tgagcggcgg ctagtctgcc agtttcgcga gcgccgcgtc
     4201 gtcgagcagg cgccgaacct catccacgag cggcttcacc cgtacgggct tggcgaggaa
     4261 gccatcgcat ccagcttcga gcgccgccgc ctggtcgcgc ggcaggacgc tcgcgctcac
     4321 gagcaggatg cgcacgtcgc ggagtcgttc catggcccgg atcggctcca cgaggtcgag
     4381 cccgtcgccg tccggcaggt gccgatcgag caggatcagc gcgggcgtct cctcggcaag
     4441 gaactcgcgc gccgcggcaa ggtccgtcgc gacctcgagg tggtacccgg ctcgggcgag
     4501 gatcgcttcg agcagcgcgg cgttgagcgg ctcgtcctcg actgccagca ggagctcgcc
     4561 gccgcccacg cgctcaggca cctcgcacgg gggcagccgc gggtccggct gccggcgaca
     4621 ttccgggcgt cacggtcaaa ccgcggggcg gagggcctcg agcgcacgca ccgcgggggt
     4681 tcgcagttcg atggcgccct cgtgctcgac catcggcacg aacagcgcga cgagcgccgg
     4741 gtcgaactgc ttgccggcct cggtgcgaag ctctgtcagc gccttgtcga tgctccgtgc
     4801 cgcccgatac gggcggtcgt ggatgatcgc gtcgtaggca tcgaccaggc cgacgatgcg
     4861 ggcgccggtt gggatcttct ccccgcggag gccatcgggg tagcccgcac cgtcccagcg
     4921 ttcgtgatgg tgtcggacga tcgaggagaa ctgcgacgcg gtcgcgagtg gctcgcagat
     4981 gcgctcgccg atgagcgggt ggaggcgcat ctcggcccat tcctggcgcg tcagcgggcc
     5041 gggcttggtg aggatcgcgt ccgagacgcc gatcttgccg atgtcgtgga ggagcgcccc
     5101 gaacacgagg cccttgagcg cggccggctc gagaccggcg ctcatgccca gctggtgggc
     5161 gaggcccgca agccgctggc agtgcatctc ggtggtcgag tccttggcct cgacggcatt
     5221 cgcgagcgcg gcgatgacgc cgtgcgcccc gaccatctcg gcggtgacgc gggcgaggcg
     5281 caggacgctg cggacgcggg ccatcagctc ggcttcgtcg tacggcttgc gcacgaagtc
     5341 gtcggcgccc gcatcgaggc cggccacgac atcgcgcgac gcggtctggc cggtgacgag
     5401 gatgatcggc agggcgaccg tgcgcggatc ggcgcggagg cgccggcaga cgtcgagccc
     5461 gttcagcccg ggcaggccga tgtcgaggag gacgaggtcg ggactctcgt cgctcgccgc
     5521 gcggacggcg tcgcggccgt cggccacggt gatcgagcgg tagccattcc gttcgaggag
     5581 gcggcccagc agagcgcggt tggccgcgtc gtcctccgca accaagacga gctcgcgttc
     5641 gaggcccagg gccatcgccg gcctccggta acgcgggaaa tccccgtcgg gggaatctgg
     5701 tacgggaggc gcgcgaaggc aacggtacga aggtcacggt gggggatggt ggagatgagg
     5761 ggactcgaac ccctgacccc cgcgatgcga acgcggtgct cttccagctg agctacatcc
     5821 ccacgcggtc gcggagtcta gcaccgggac cttccgcggc ccgcgtgtgg ccgtcttgca
     5881 gccctggaca cctcgccgct gggctcccgc ggctcctcgt cccgccgtcg ttcatcagat
     5941 gcacgatctg gcggacgaac agtcggaatg gctttggagc gcccgaattg gcctcgtttc
     6001 gacgccgtca gccgccgcag cgttcattgg atgcacgatc ttgcgtctgg acccgccggt
     6061 ccggtcgttc gcccgccgta tcgaacattc aatgcagggt tggggcgaga acgagcccac
     6121 gacttggccc gcatacctct tgattcattc gaacagatgt tcgatactga acggcatgac
     6181 ggctccctcg accgacctga gcacggccct cgcgaccctt cgatcgcgct ggggtgcggc
     6241 cgctcccggg ttcgtgggcg aggtcgtcgg ggcgctcgcc accgtcccgc tcgaggacct
     6301 cgaccagccg ggttcgggac ccatcggggt gccgcagcag cacgctgatc ccgaccggat
     6361 ctactcgacc ggattcgccg agctcgatgc catcctcggg cccggcggcg tgccccgcgg
     6421 gatgggcgtg tcgctgcgcg gcgatgcctc gagcgggcgg acgacgctgg cgctgcggct
     6481 cgtggccgag gcgcaggcgg cggggtcgat cgtggcctgg ctggacctga ccgcggcgct
     6541 cgacccggtc gaggcggttg cgcggggcat ccggccggag tggctggtcg tcctgacccc
     6601 ggccgatccc gacgagggcc tggcgatcgc cggctcactg ctcgcgggcc gggcggtgga
     6661 cctgctggtg atcgacctgc cgagcaagct gtcgaagacg ttcgctgatc gactcgggcg
     6721 gttggcggcg ctcgcgcgtc gggccgggat caccctcctg gtgctggagc cgccacgcgc
     6781 caccggcggc ctggcggccg cagtcggcga ggcgacgggg atccggctcg agctggcccg
     6841 tcgatcgtgg atccggctcg gccgggacgt cgtcggccag cgaacggagg tgatcgtcgc
     6901 ccgcaatcgg gctggcccac cgggccggcg agcgaccctc cgaatcctgt atgcggacgg
     6961 aggtgcgcgc gacgcctgcc tccgacgcga cgacctcttg catgaccggc ggctggttca
     7021 gccggccgga gtgcaaggcg cggccaagcc gggtgagcga acgcacttgg agtgcgtgag
     7081 cgagcccggc gcaggtcgca acgccgcaat acggccagat caaccagccg cccccatccc
     7141 agtgcccggg aaacggaacg atgcgactcc tccatcttca tcgcccacac ctcctcctcg
     7201 acttcgcgag gcggcgcctg ccctccggct cgtggccaca ggggccggtg atcctcggcg
     7261 gagcgccgtg gtcgaacggg aacgtgctcg acgcggatcc gaccgcgcgg gcgctcggcg
     7321 ttcgccgcgg gatgccgctc gggtcggcgc accggctggc gccggaggcg acgttcctcg
     7381 acgccaagcc cgaggctgat cgagccgagc tcgaggccgc cttcgaacgc ctcgccgggt
     7441 tcagcccggc gatcgcgggg tcgaccgagc cgaccgacgt cgcgttcggt ctgctcgagg
     7501 cccagatcga tgggctcgaa ggcctgtggg ggctcgagcc ggcactggtc gagcggatcg
     7561 gcggagcgct cgaggagccg ctggcgggcc ggccgcgggc ggggattgcc ggcacgcgct
     7621 tcgccgcgac cgtggcggcc ctcgccgccg agcccggctc gctcgtcagc gtcccgcccg
     7681 gcgcggaggc cgagttcctc gccccgctgc cggcgtcgct gctcacaccg gacgccgaca
     7741 tccgcgcccg gctgcgccgc ttcggtcttc gccaggtcgg gcaggtcgcc gcccttgcga
     7801 cgtcagcact ggtcgcgaga ttcggcgagg aaggcgcccg catcgggtct cgctcccgtg
     7861 gcctcgaggt cgatccgttc cggccccgcc gcgcacccga gcgcctggtc ctcgggctgc
     7921 cgatcgagcc cgccgtcgcc gagctggagg cgatccggtt cgtgctccga cgcctggccg
     7981 tcgcactcgc gacgaatctc gccgcccgcg gcctcgccgc cgagcaggga cggctgcgcc
     8041 tcgagctgga tctcgctttc gcgcgcaagg gcaccgcgcc ggagctcgcc gtcgagaccc
     8101 gctttcccga gcccaccgcc gaccccgagg cgatcgagcg gctgctcctc gccaagctgg
     8161 agcggacacc gcccccggcc gcggtcgccc ggctggagct ggagctcaac ggcgcgagcc
     8221 cacaggccgg ccagcagctg cccctgttca tcccgcaggc actccacggc gcgcgcctcg
     8281 cctggcagct ggcgcgcctc gccctgacct acggcgagga ccgcatccac agggtcgaga
     8341 tcaccgaccc cgaggcgccg ctgcccgagc accgctgggc ctggcgcgac gtgagcgccg
     8401 gcacgtgacc gcctcccgcc actccctcgg gagtccgctg tgactcgcct cctccgtggc
     8461 cacccatcga tcaacgtcga ggtcgatgac gggcgcgtcg tggccatcgc ctgggccgag
     8521 cggcgcgagc cggtcgaggt ctgcaatcgc tggcgggtcg aggaatcgtg gtggcgtgag
     8581 ccgatcgcgc gcgactactt caaggtcgtc ggcgagcact ggctcgcgct catctacttc
     8641 gatcgagtcg ccggcagctg gcacctagag cgtttgtacg actgatcgtc gaccatgcgg
     8701 cccatgcccg cgttgggtcg agctcagacc gggctcgacg ccaccactgc cgacagcacc
     8761 gccagcgttg ccgcaagcgg cgcccagccg aagcgctccc acgggctcga cgacgcgacg
     8821 ttgacgagcg agccgagcgc ggacagcacc acgagcaccc aagcgccaac ggtcaaggtc
     8881 acttccggga gcgggacatc gatgagacca gctcgcccga ggatgatcgc cgcagccacg
     8941 agccagatga cgacggccac cgcgctcgat cgtcgcagcc gagccggcag caccgcggtt
     9001 cgaccgcccc acgccgcccg ccccagcggg gcgcccgcag cgagggcagc ctggaacacc
     9061 gcgatcacca cgaagccaac ggccgagatg gcggccgcag actcgacgct cacttcgcga
     9121 gaaccggccg aagcctgaac tgcgtgctca ttcgaacggg cagccggcgc cgtacagcac
     9181 gcgcgccacc acctgattcc cgagtggcgc gagccgcacc tcgatctggc agttgccggg
     9241 caccgacacg acgaaggcgc catcctcgag cggaccatcc acatcgaccg ccgcgaagcc
     9301 ggccgcctgg aagctggttc ggaagaagtc ggcggcagcc tgcgcggtcg attccgccgg
     9361 gagggcgagc tcggccgtcg ccgggccgct acccgtctcg gtcgggcttg ctccatcagg
     9421 gatcgggaac catggcggca acgcgtccca gatgcgaccc cattcggtgt cggtctgggt
     9481 cggcaggctc gggccgaggg tcggaggtgc ggtcgggtcg ccgtgcgacg tcccgatcgg
     9541 tccggtggcc gtcggggtcg cagagcccgc ggttggggtc ggtgtacatc cgccgacgac
     9601 cggaaccgca agcaagatga gcagcgccgt cagggcgatg aagccgcggt gcatgcgatc
     9661 gatggtagcc acgtctcgtt ctccgcagac gtgaaaggac cccgtcgccc ccagtagcga
     9721 cggggtcctt ccgcttggtc tgtcactcgc tcaccgagtg gccgaccggt cgacggccgg
     9781 ctgtcgagcc cgccctcgcc acgtcccgga gttggaggtc cccggggttt gcgtgcggtt
     9841 accgagcgct cgccgccaat gccgccggga ggaggacgag gatgaggaga accgcgagca
     9901 ggagcatcac ggcggtcctc gtcaggacgg cggccaggct gcgattgtcg atgtccacgg
     9961 cgttcagacg ccgtaagccc gcactgtgtt gcgtcgccag agagagaaca tttgttctat
    10021 agtgacgggc ccccctttgt cgcgagaaac gcggacgggc cagccgatgt cacgctccta
    10081 cgccgagctc cacagccaca gcaatttctc gttcctgcac ggcgcctcca cggtcgagga
    10141 catgaccgag cgagcggccg agctgggcct ctgcggactc gcggtcaccg atcacgacgg
    10201 gctgtacggc gtggtgcgct tcagcaccgc ggcggctgcg gcggggatcc gcccgatcat
    10261 cgggatcgag atcgagctgg tcgacccggc tgcgcccgac ccgggcggca tcgtcgttcc
    10321 ggcccggcgc ggggcacggg gccgagcgcc ggcactcccc gggctcctcg cggcgggtgc
    10381 cgacctccag ccgatcgagg gcgtgccggc acggccccgt ccgacccggg ctcgcctgcc
    10441 ggggcatcga gcggtggtca aggaggacct gcgggggatc ggcggccgcc agcgcgggcc
    10501 gcatctcgtc ctgctggcca gggatgcgac gggctatcgc agcctgtgcc ggctcgtctc
    10561 ccgtgcgaat ctcgacggga ccaagcgcgt gccgaggttc acccacgcgc tgctcgagcg
    10621 gcatgccgag ggcctcctcg cgctctcggg agggcgggag agcgagatcg cgcgacgcct
    10681 ccaggtcggt gatcgagccg gcgcgcgagc ggtcgccgag cgctacgcgg cgctgtacgg
    10741 cagtgccgaa ttccgctccc gtgccggcac cggtcgcggc tccggtggcg gccgcgggcc
    10801 cgtcgcgggc gattcgccgg ccacggctgg cctcttcat
//
LOCUS       DATBSW010000003         8515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1163959, whole genome shotgun sequence.
ACCESSION   DATBSW010000003 DATBSW010000000
VERSION     DATBSW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163959"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..867
                     /locus_tag="VIF63_00100"
     CDS             283..867
                     /locus_tag="VIF63_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237815.1"
                     /translation="MIVVGILTPASGADSVVEIGRRAAGTGARTEVIGVAPASVDGDR
                     QLLALASAGVGHATVIRSAAEGIEPADLDLALRYLPDVRAIALVRPAAALLATALSAA
                     SWSGATLIVVGPLDADVTTLVEAASSGAAAGSGGTTSAATRSMSPILLEPPARDPDGA
                     FAGVVAALAVRLDAGEDPAAAWQSTVSALALDPA"
     gene            complement(1193..2017)
                     /locus_tag="VIF63_00105"
     CDS             complement(1193..2017)
                     /locus_tag="VIF63_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY8237816.1"
                     /translation="MTQQRPTSELAEIFEAEDRIDAPGGRFNLAPTDDAAVVVQREEA
                     RAITAYRWGLIPHWSETPKTGNRMFNARAETIDRQPAFRYAFSKRRCLVPVDAYYEWK
                     RAGSVRQPYAFVSPDGRPIALAGLWAGWKDEDTGEIIRSFTIVTTSANEMMAPIHDRM
                     PVMLPEDAWERWLDPSRTAGAGLSELKGLLVPSDEGWLEMFPVSRLVNSVQNDGPELL
                     ERIPDDAVAGGPGDPGVPTHDPGPPRRSRARGRAPSADGPGLFDDPTELERDDPAD"
     gene            2054..3247
                     /locus_tag="VIF63_00110"
     CDS             2054..3247
                     /locus_tag="VIF63_00110"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8237817.1"
                     /translation="MTEPGDDHFRDPQLDAALATPGPLSPDVARRLIERGRAKLEASE
                     PMQAAADFQRAVGHEDPAITGAALLGLGDALYRLDNEPQAGAAWTAVTRMPDNPSTYQ
                     AWRNLAGVLVRAGDLTSAVEAYRQADRRAPAEDKPEIAARLGWLAKETGNAGAANRYF
                     ARSRPTVAGIGISLIVLAVTVVISFLAESSPQLLQTLWLDRSDVLAGQLYRLASVTLV
                     HDGPIHLFFNMYALYVVGPIVEQAWGKRLFVFLYLLTAVAASTASFLNTPGPAVGASG
                     AIFGLVGVILAGTRAHHPMLDRRARAIVPQLGMFVLINLAFGFAVGGLIDNAAHVGGL
                     VAGLWLGFVVPPGKVPTLRSAWQNLRGEPAQRSPLLVAAGVLLLVGVIFAALAVGFET
                     FGRLR"
     gene            complement(3253..4740)
                     /locus_tag="VIF63_00115"
     CDS             complement(3253..4740)
                     /locus_tag="VIF63_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8237818.1"
                     /translation="MPRSLGGRLVGAFAILALAIVLTVGGALFVVLRGLHHDATLGTL
                     SDVATSVLPQVRESIGTGQLRGTIEEIRQRLAEQPVPIELLLVSPDGTVKSADGTFSG
                     RTLLDDAVAVGETVQGEIVAADGKSRWLYAATAIRRQGAATPRGVAFVTLDRSGALAL
                     ADVGRTIPVVALVILLIGTPIAIALSRSVTGPLRRLADTAADIPAGRAAALPLSGPAE
                     VRELTGTFNAMTAELDATRQRERDLLANLRHDLRTPLTVIAGFATALRDGTAQGEAAE
                     AAAKAIEEEAERLERLVAQIGAIERIGSGDDAIRPEMLDARLLIADTVARFRARATAS
                     GVDLAPAEPDATEPLALAADRLALERMLGNLVENAISAASSGGSGGSSRTEEGAGGEV
                     RIAAALERAPDGTESIRIDVLDNGPGFPDGSAGRAFERFWRGDPARAGSGSGLGLAIV
                     RELALAHGGSAHAQNRAKGGARVGVTLPRVPWPAQPRASDGARPD"
     gene            complement(4744..5454)
                     /locus_tag="VIF63_00120"
     CDS             complement(4744..5454)
                     /locus_tag="VIF63_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8237819.1"
                     /translation="MKSILIVDDERNIVDLVRLYLEKEGYAVVAAGDGEQALALHARH
                     EPDLVILDLMLPKKDGFEVCREIRKRGDTPVIMLTARGDDVDAIVGLELGADDYITKP
                     FNPRALVARVKAVLRRADGSVKGGRPFEVGDLRVDPRRRDAYVGERRLSLRPREFDLL
                     VALARDPGVVLTRDVLLEGVWGTDFPGETRTVDVHVAELRKALAPDGPEIETARGYGY
                     RMVPAARAPLPPPDTDPA"
     gene            complement(5728..6105)
                     /locus_tag="VIF63_00125"
     CDS             complement(5728..6105)
                     /locus_tag="VIF63_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8237820.1"
                     /translation="MPRRAAEGIELLADPTRRRIVALLAARVWRPADIADAIGLGRPA
                     VSRQLRLLRSAGLVRWRWSVIDLRSRDYFIDPAMKDPIIAWLAGVDLRSIRPIFSPSW
                     SPPARVHRRRHDAKELRVDREVD"
     gene            6457..7443
                     /locus_tag="VIF63_00130"
     CDS             6457..7443
                     /locus_tag="VIF63_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237821.1"
                     /translation="MFATLLGALPTPDGDGSPVELAVRAQEEAGLEPITDGRLGESTF
                     AMFRDALAGGAATLPSSVAAWQLIAGLTDRAVKQSLPGPYTLARRLGVGMAGDEGSNV
                     RARRTHAIAEALASEVQALVAAGCPLVEIEEADAHLIGADEVERRLFRQAHRRLTDGV
                     DLADGVDLGKGVLRTHLSLSIVGGSAAGAGIETILDAPYASLAVDLIAGPENWNLVTR
                     APGDRGIVAGAMGARPEGDEPKEILLWAAHYAASTSGRGLARVGLGSAGSYANLTWAQ
                     AVRKMRLLGTAAKLAELPPGDDLTGQVDPQAVSARRAALGRNAPKPPKRPRR"
     gene            7460..>8515
                     /locus_tag="VIF63_00135"
     CDS             7460..>8515
                     /locus_tag="VIF63_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8237822.1"
                     /translation="MTTRFWHGFADMHVIKDREVVIASGDGATITDTNGKTYIDATAA
                     LWYCNVGYGRREIADAVAEQLVNLHAYSSFGAYTTEATLRIADRLAALSPIPNAVVYL
                     ASGGSDAIDTAGKIARRYWDVVGKPNKTLLVSREHAYHGMHAMGTSLGGMPVMRNGYG
                     GEPFINEVVHVPAMDVDAVARLFEQRRDELAAFFGEPVIGAGGVYPPTESYWREISAL
                     CKRFDVLLIADEVITGFGRTGHWWGTQRYGIEPDMITFAKGVTSGYMPLGGILVGERI
                     RAPFWDEPIAGAVFRHGYTYSGHSGAAAAALANLDIIEKEGLVERVATLEPVLDKAVR
                     RLQGTPLVGEIRTVGLTA"
BASE COUNT         1192 a         3063 c         2984 g         1276 t
ORIGIN      
        1 ccggcaaccc acagctccgt gccgatcagc tcgacatact gctcgccgcc gccctcgccc
       61 tcgtcggcaa acgccggcgc gccggtgccc caggcatcga ccgaccgatc gaatgtcggt
      121 ggtgcatcgc cccattcgcc catctgtcgc gcaccccgtc gaatggcgcc ggcccccgct
      181 ggcccggctg cgtggcgcca gtctgccccg tcgatgcccc gcgtgcacga tccgttcgta
      241 ccagccggcg ttgccacgag cgccatgggc taggctggcc gcgtgatcgt ggtcggcatc
      301 ctcacacccg cctcgggcgc cgattccgtg gtcgagatcg ggcgtcgcgc ggccggcacg
      361 ggagcgcgca cggaggtcat cggcgtggcg ccggcaagcg tcgacgggga tcggcagctc
      421 ctcgcgctcg cctccgccgg ggtgggccac gcaaccgtga tccgctccgc agccgagggc
      481 atcgaaccgg ccgacctcga cctcgccctg cgctacctgc ccgacgtgcg ggcgatcgcc
      541 ctcgtccgcc ccgcggctgc gctgctcgcg accgcactct ccgccgcctc gtggtccggc
      601 gcgaccctca tcgtcgtggg cccgctcgac gcggacgtca cgacgctggt cgaggctgca
      661 agctcgggtg ctgccgcggg ctcgggaggg accacgagcg ccgccacccg ctcgatgagc
      721 ccaatcctgc tcgagccgcc ggcccgcgat cccgatggcg cattcgcggg cgtcgtcgcc
      781 gcgctcgccg tccgcctcga cgccggcgag gatcccgccg ccgcctggca atcgaccgtt
      841 tccgcgctcg ccctcgaccc ggcctgactt cagcgcgcga cccacgggga tgtcgccggg
      901 gtcgctgcgt tgctccaaat cgatcgaatc ggcgcggaaa tgcgccaaat gccgtcctgg
      961 ggcacgttgg gcatccacct gacccgaatc cgcctcgaat cgtccccttc gtccgttggt
     1021 tgccgcgcga ttcgtgctca gccctggccg gctcgtgctc ccagtttcgg cttcgcgcgc
     1081 ggagcagcgc cgaaagcgat ggccaacgtg cccacggcgc acaaatggcg caatcgggca
     1141 ggtccaggct ctgccgcgcg gatctgggag gcgcgcaggt gtcgcccgcc gttcagtctg
     1201 cggggtcgtc gcgctccagc tcggtcggat cgtcgaacag gccgggtccg tccgcactcg
     1261 gcgcgcgacc tcgtgcgcgc gagcgccggg gaggtccggg gtcgtgcgtt ggcacgcccg
     1321 gatcgcccgg gccgccggcg accgcgtcgt cagggatccg ctcgaggagc tccggcccgt
     1381 cgttctggac cgagttgacg agtcgcgaga ccgggaacat ctcgagccag ccctcgtcgg
     1441 acgggacgag caggcccttc agctcgctca ggcccgcgcc cgcggtgcgc gacgggtcga
     1501 gccagcgctc ccacgcgtcc tcggggagca tcaccggcat gcggtcgtgg atcggggcca
     1561 tcatctcgtt ggctgacgtg gtgacgatcg tgaacgagcg gatgatctcg cccgtgtcct
     1621 cgtccttcca gccggcccaa aggccggcga gggcgatcgg gcgaccatcg gggctgacga
     1681 acgcgtacgg ctggcgaacc gagccggcgc gcttccactc gtagtacgca tcgaccggca
     1741 ccaggcagcg gcgcttcgag aaggcgtacc ggaaggcggg ctgtcgatcg atcgtctcgg
     1801 cgcgagcgtt gaacatgcga ttgcccgtct tgggcgtctc cgaccagtgg gggatcaggc
     1861 cccagcgata ggcagtgatc gcgcgcgctt cctcgcgctg gacgacgacc gccgcgtcat
     1921 cggtcggggc gaggttgaac cggccgccgg gcgcatcgat gcggtcctcg gcctcgaaga
     1981 tctcggccag ctccgaggtc gggcgttgct gcgtcatccg tccacacatg gcatctctag
     2041 tatcgtccgc cgcgtgaccg agccggggga cgaccatttc cgcgaccccc agctcgatgc
     2101 cgcgttggcc acgcccggac cgctcagccc ggatgttgcc cgccggctga tcgaacgcgg
     2161 tcgagcgaag ctcgaggcca gcgagcccat gcaggcagcc gccgacttcc agcgcgccgt
     2221 cgggcacgag gatccggcga tcaccggtgc agcgttgctc gggctgggcg atgcgctcta
     2281 tcgactcgac aacgagcccc aggcgggtgc cgcctggacg gcggtcacgc gcatgcccga
     2341 caacccatcg acgtaccagg cgtggcggaa tcttgccggc gtgctggtcc gcgcgggcga
     2401 cctgacgagc gcggtcgagg cgtaccgcca ggccgaccgg cgcgcgccgg cggaggacaa
     2461 gcccgagatc gccgcgcgac tcggctggct cgcgaaggag accggcaacg ccggcgccgc
     2521 gaaccggtac ttcgcccgaa gccggcccac ggtggcgggc attggcatca gcctcatcgt
     2581 tctcgcagtc acggtggtga tctcgttcct cgccgagtcc tcgccgcagc tgctgcagac
     2641 cctctggctg gatcgctcgg acgtcctcgc cggccagctc tatcgcctcg ccagcgtgac
     2701 gctcgtccac gacggcccga tccacctgtt cttcaacatg tacgcgctct acgtggtcgg
     2761 tccgatcgtc gagcaggcct ggggcaagcg gctgttcgtg ttcctgtacc tgctcaccgc
     2821 ggtcgccgct tccaccgcaa gcttcctcaa cacacctggc ccggcagtcg gcgcctccgg
     2881 ggcgatcttc gggctcgttg gcgtgattct cgccggcact cgcgcccacc acccgatgct
     2941 cgaccgccgg gcgcgggcga tcgtccccca gctcggcatg ttcgtcctga tcaacctcgc
     3001 gttcgggttc gccgtcgggg gcctgatcga caacgccgcg catgtcggcg ggctcgtcgc
     3061 gggcctctgg ctcgggttcg tggtgccgcc cggcaaggtg ccgaccctcc gctcagcgtg
     3121 gcagaacctg cgcggcgagc cggcccagcg gtcgccgctg ctcgtggctg cgggggtgtt
     3181 gctcctggtc ggggtgatct tcgcggcgct tgccgtgggc ttcgaaacct tcgggaggtt
     3241 gcgctagcgc ggtcagtcgg gcctcgcgcc gtcgctggcc cgaggttgcg cgggccacgg
     3301 cacgcggggg agtgtcactc cgacgcgggc gccacctttg gcgcggttct gggcatgcgc
     3361 gctgccgccg tgggccaacg cgagctccct gacgatcgcg agcccaagcc ccgagcccga
     3421 gccggcgcgt gcggggtccc cgcgccagaa gcgctcgaat gcacgaccgg cggagccgtc
     3481 cggaaagccg ggcccgttgt cgagcacgtc gatgcggatg gattccgttc cgtccggcgc
     3541 tcgctcgagc gccgctgcga tccggacttc gccgcccgcc ccttcctcgg ttcggctcga
     3601 tccgccggaa ccgccgctcg acgcggcgga gatcgcgttc tccaccaggt tgccgagcat
     3661 ccgctcgagg gcgagccgat cggcggcaag ggcgagcggt tccgtggcgt caggctcggc
     3721 cggcgcgagg tccaccccgg aggccgtggc ccgggcacgg aagcgagcaa ccgtatcggc
     3781 gatcaggagg cgcgcgtcca gcatctccgg ccggatcgcg tcgtcacccg agccgatccg
     3841 ctcgatcgcc ccgatctggg caacgagccg ctcgagtcgt tcggcctcct cctcgattgc
     3901 tttcgccgct gcctcggcgg cctctccctg ggccgtgccg tctcggagcg ccgtcgcgaa
     3961 gccggcgatc accgtcagtg gcgttcggag gtcgtggcgc aggttcgcga gcaggtcgcg
     4021 ctcgcgctgg cgggtcgcgt cgagctccgc ggtcatggca ttgaacgtgc ccgtgagctc
     4081 gcgcacctcg gcgggaccgc tcaggggcag cgcagcggca cgccccgctg ggatgtccgc
     4141 cgccgtatcg gcaagcctcc ggagcggccc ggtcacgctg cgcgagagcg cgatcgcgat
     4201 tggcgtgccg atcagcagga tgacgagcgc gacgacggga atcgtccggc ccacgtccgc
     4261 gagcgcgagc gcgcccgatc ggtcgagcgt cacgaaggcg accccgcgcg gcgtcgccgc
     4321 accctggcgc cggatcgccg tggccgcgta cagccaccgg ctcttgccat cagccgcgac
     4381 gatctcgccc tggaccgtct cgccgactgc aacggcgtcg tcgagcaacg tcctgcccga
     4441 gaacgtgcca tcggccgatt tcaccgtgcc atccgggctg acaagaagga gctcgatcgg
     4501 caccggttgc tcggccagcc gctggcggat ctcctcgatc gtgccccgga gctggcccgt
     4561 gccgatgctc tcgcggacct gcggcaggac gctggtcgcg acgtcggaca gggtgcccag
     4621 ggtcgcgtcg tgatgcagcc cgcggagcac gacgaacagc gcgccaccga cggtcaggac
     4681 gatcgcgagg gcgagaatcg cgaacgcgcc gacaaggcga ccgcccaggg agcggggcat
     4741 cgatcaggcg gggtcggtgt cgggcggggg caggggggcg cgcgccgcgg gcaccatccg
     4801 gtagccgtag ccgcgcgcag tctcgatctc cggcccgtcg ggcgcgagcg ccttgcggag
     4861 ctcggcgacg tgcacgtcga ccgtccgcgt ctcgcccggg aaatcggtgc cccatacccc
     4921 ctcgagcagc acgtcgcgcg tcagcacgac gccggggtca cgggcgagcg cgacgagcag
     4981 gtcgaactcg cgtggccgaa gcgagagccg ccgctccccg acgtaggcgt cccgccgccg
     5041 cggatcgacg cgcaggtcac cgacctcgaa cggtcgtccg cccttgaccg acccatctgc
     5101 tcgccgcagt accgccttga cccgcgccac cagcgcccgc ggattgaacg gcttcgtgat
     5161 gtagtcgtcc gcgccgagct cgagcccgac gatcgcatcg acgtcgtcgc cgcgcgccgt
     5221 gagcatgatc acgggcgtat cgccccgctt tcggatctcg cgacagacct cgaagccatc
     5281 cttcttgggc agcatgaggt cgaggatcac gaggtccggc tcgtgtcgcg catgcagcgc
     5341 aagcgcctgc tcgccatcgc cggcagcgac gaccgcgtat ccctccttct cgaggtacag
     5401 gcgcacgaga tcgacgatgt tccgctcgtc atcgacgatc aggatcgact tcaccggcgg
     5461 gagaatacgc ccgcccgcgg ctcgcccggc gaccgaacgt aaaggccatg taaggagcgc
     5521 cccggacgtc gcggttgccg ccgcatcgcg gtcggtcgcc gccggtcgcc gttgccaatc
     5581 gttcgccgct cgcgttcgcc ggcgccggtc accgttccag tcgccgcgcc gttcgaccgt
     5641 cgtccgttca tcccctggcg cccgttttcg agatggttcg aatgaagccg aaccatcgaa
     5701 gatgaggcgg ccatggtgaa ggcgacctca atcgacctcg cggtccacgc gaagctcctt
     5761 tgcgtcgtgt cgccggcggt ggacacgcgc cggcggcgac cacgagggac tgaagatcgg
     5821 gcggatgctc cgcaaatcga caccggcgag ccaggcgatg atcgggtcct tcatagctgg
     5881 gtcgatgaag tagtcgcgac tgcgcagatc gatgaccgac cagcgccatc gaaccaggcc
     5941 ggcggacctg agcagtcgaa gctgccggct gacggcgggc cgcccgagcc cgatcgcatc
     6001 ggcgatatcc gccgggcgcc agacacgagc agccaggagc gcgacgatcc gccgccgtgt
     6061 cggatccgcc aggagctcga tgccctcggc cgcgcgtcgt ggcatcactt gatcctggcg
     6121 agggccgctt gtcgatcgct tatctccgtc ggccggatct cgggcttcgc cacggttcga
     6181 cgtcgggcct tgcgacggtc cgatcacggg ctccgccccg atggttcggc ttcgttcgaa
     6241 ccaactcgaa aaggggtctc aggggaggaa atgccgacgg agccgatcag ggctgaagct
     6301 gtcgacaacg gttgtggctg tcgacaacgg ttgtggctgc acgcagcggc tgcggctgca
     6361 gacagccgct ccggctcttg aaggcggctg aagctgccga ccgccgctga acgctgccgc
     6421 ccacggcacg gcgacatcgg ctctacgatc cggcgcgtgt ttgccacgtt gctcggcgcg
     6481 ctgccgacgc ccgacggaga tgggtcgccg gtggagctgg cggttcgggc gcaggaggag
     6541 gccgggctcg agccgatcac cgacggccgg ctcggggagt cgacgttcgc gatgttccgg
     6601 gacgcgctcg cgggtggggc agcgacactg ccgtcgtcgg tcgcggcgtg gcagctcatc
     6661 gccgggctga ctgatcgagc ggtgaagcag tcgctgccgg ggccgtacac cctggcgcgg
     6721 cgactcggtg taggcatggc gggcgacgag ggctcgaacg ttcgggcaag gcggacccat
     6781 gcgatcgccg aggccctcgc gagcgaggtg caggcgctgg tggcggccgg atgtccgctc
     6841 gtcgagatcg aggaagccga cgcgcacctg atcggcgctg acgaggtcga gcggcggctg
     6901 ttccggcagg cgcacaggcg cctcaccgac ggtgtggacc ttgccgacgg cgtggacctt
     6961 ggcaagggcg tgctgcgcac gcacctctcg ctgtcgatcg tcggcggctc ggcggcgggc
     7021 gccggcatcg agacgatcct cgatgcgccg tacgcgagcc tcgcggtcga cctcatcgcc
     7081 ggccccgaga actggaacct cgtgacgcgt gcgccggggg atcgggggat cgtcgccggc
     7141 gcgatgggcg cgcggcccga gggcgacgag ccgaaggaga tcctgctctg ggcagcccac
     7201 tacgcggcat cgacgagcgg tcgcggcctc gcgcgagtcg gtctcggctc ggcgggttcc
     7261 tacgcgaatc tcacctgggc gcaggccgtc cgcaagatgc gcctcctggg caccgccgcg
     7321 aagcttgccg agctgccccc gggcgacgac ctgaccggcc aggtcgaccc gcaggcggtc
     7381 agcgctcgtc gcgccgcgct cggtcggaac gccccgaagc ccccgaaacg accgcgccgt
     7441 tgatacgctt cgcgatccca tgacgacccg cttctggcac ggctttgccg acatgcacgt
     7501 gatcaaggac cgcgaggtcg tgatcgcatc cggtgacggc gccacgatca ccgacacgaa
     7561 cggcaagacc tacatcgacg cgaccgcggc cctctggtac tgcaacgtcg gctacgggcg
     7621 gcgcgagatc gccgatgcgg tggcggaaca gctcgtgaat cttcacgcct attcgtcgtt
     7681 cggggcctac acgaccgagg caacgctgcg aatcgccgat cgactggccg cgctgtcgcc
     7741 gatcccgaac gcggttgtct acctggcttc gggcggctcg gacgcgatcg ataccgccgg
     7801 caagatcgca cggcgctatt gggacgtcgt gggcaagccg aacaagaccc tgctcgtgtc
     7861 gcgcgagcac gcctaccacg gcatgcacgc gatgggcacc tcactgggcg ggatgccggt
     7921 catgcgcaac ggctacggcg gcgagccgtt catcaacgag gtcgtccacg tgccggcgat
     7981 ggacgtcgac gcggtggctc ggctgttcga gcagcgacgc gacgagctcg cggcgttctt
     8041 cggcgagccc gtaatcggcg ctggcggcgt ctacccaccg accgagtcct actggcgcga
     8101 gatctcggcg ctctgcaagc gcttcgacgt cctgctcatc gccgacgagg tgatcaccgg
     8161 cttcggacgc accggccact ggtgggggac gcagcgctac ggcatcgagc ccgacatgat
     8221 cacgttcgcg aagggcgtca cctcgggtta catgcccctc ggcgggatcc tcgtcggcga
     8281 gcggattcgc gcgccgttct gggacgagcc gatcgccggc gcggtgttcc gccacggcta
     8341 cacgtactcg ggccactccg gcgccgcggc ggcagcgctt gcgaacctcg acatcatcga
     8401 gaaggaaggg ctcgtcgagc gggtcgcaac gctcgagccg gtgctggaca aggccgtgcg
     8461 ccggctccag ggcacgccac tcgtgggcga gattcgaacc gtcggcctga ccgcg
//
LOCUS       DATBSW010000004        22158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_987565, whole genome shotgun sequence.
ACCESSION   DATBSW010000004 DATBSW010000000
VERSION     DATBSW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22158
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987565"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VIF63_00140"
     CDS             <1..229
                     /locus_tag="VIF63_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8237823.1"
                     /translation="IGQTGLRIAPDLYIACGISGAIQHIVGCKAAKRILAINTDPDAP
                     IMAVADYAVIGDLSTIVPAITAEIERRRVPA"
     gene            complement(312..641)
                     /locus_tag="VIF63_00145"
     CDS             complement(312..641)
                     /locus_tag="VIF63_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237824.1"
                     /translation="MAQPSANPLDGLPLRLLEGETVTVLAEAVGAKIVVTDRRLAIAA
                     DDRVALDIGFEGLRRIQFDIERTRPATLVIVPEHPAHEPQVLAIPPERYDEVTQALAE
                     VGRRLAE"
     gene            complement(801..2054)
                     /locus_tag="VIF63_00150"
     CDS             complement(801..2054)
                     /locus_tag="VIF63_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8237825.1"
                     /translation="MTGASPLDAGEALPATANATGETTDSRWVAVVAALILVGTALRP
                     QALIIGPLVGKVQADLGMSHGIAGLLSTIPVLCMGLLAPFGPVLAASIGPKLGATICV
                     LLVGVFGVARGLLPDTFTVLLATVGIGVGMAVVGPILPMIVRARLPKQPAAGTGAYVV
                     GFVIGGTITAAVAVPLADAFGGWRGAFVIVSSAAFVSMAAWVWLMPRDAGHVRVMPKL
                     PRLPWRRPSAWLLGLIFGSQSILFYGCITWLASAQIERGWTAAQAGSLVALLTGVGLI
                     ATLAVPVLADRFGTRRSQLTIAGLLSVGGALVIALSSAEPPGSIVIYAATALLGLGIG
                     GYFPLALTLPVDVASDADDAASISALMLLIGYALAATAPVVLGLVRDATGNFVGVLWV
                     LVGVAAAMIPLAIALNPARLRRAGG"
     gene            complement(2051..2821)
                     /locus_tag="VIF63_00155"
     CDS             complement(2051..2821)
                     /locus_tag="VIF63_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8237826.1"
                     /translation="MSSPTIGVQSIRRAFAVLRALADGPLGVSEVAIASRIPKSTAAR
                     LLAALASEGAVEQVPGERRYRLGPDLLAIASGLGETWDIVAIARPTLVDLTTELGEAA
                     GVSIRDGWTVQYVDQLEPPNPNPVAVRDWTGTRIPLHAVSSGQVFLAQLAAPVLARYL
                     AEPLEAFTARTLTDASALLERMRDVRRDGHAWVRDEYAVGISSVAAPIADARAEVVAA
                     VHVHGPSYRFPAPGDEDRIASAVRAAAARIGARLRAGS"
     gene            2939..3637
                     /locus_tag="VIF63_00160"
     CDS             2939..3637
                     /locus_tag="VIF63_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEY8237827.1"
                     /translation="MADGTQDFDLSALADDELTEQMHNDLYDGMAPEIVQGTNILLER
                     GWGPDKVLNDALVEGMRIVGIDFRDGILFVPEVLLAANAMKAGMAILRPLLAETGAKP
                     IGKVVIGTVKGDIHDIGKNLVAMMLEGAGFEVYDLGINTDADKFIAALELHKPDILGM
                     SALLTTTMPYMKVVVAELVKRGIRDDYIVLVGGAPLNEEFGKAVGADAYCRDAAIAVE
                     TARALVLERRARVA"
     gene            3678..4286
                     /locus_tag="VIF63_00165"
     CDS             3678..4286
                     /locus_tag="VIF63_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY8237828.1"
                     /translation="MTAPRAPLVIGCGALAVELIALTRRAGLPAMDLTCLPASLHNRP
                     ERIPGAVAARIRRARADGYDRIFVAYADCGTGGMLDRVLEAEGVARLEGAHCYEVYAG
                     RAAFATLADEEPGTFYLTDFLVRNFDRLVIRGLGLDRHPELLSIYFGNYRRLVFLAQT
                     DDAQLTARAEAAADRLGGLTFERRLVGLGELATSIASFGKAA"
     gene            4286..4588
                     /locus_tag="VIF63_00170"
     CDS             4286..4588
                     /locus_tag="VIF63_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY8237829.1"
                     /translation="MASLITILWRDIPLQVIARDRREAHKVVLHPRFQVAADKAAMKA
                     GKRAASDYIDEMRRDARECGDDLEAEANGEAERLEAAYTKDVLATLIAAGGVGQRL"
     gene            4674..5681
                     /locus_tag="VIF63_00175"
     CDS             4674..5681
                     /locus_tag="VIF63_00175"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8237830.1"
                     /translation="MTITLARRSITHTTISSATREVVIGFDRPFTIIGERINPTGRKL
                     LAEEMKAGDFSRVERDAIAQVEAGAHMLDVNAGIPLADEPAILARTIRLVQSLVDVPL
                     SIDSSIVEALEAGLAAYIGKPLVNSVTGEEERLERVLPLIRKYGAAVVAISNDDTGIS
                     EDPDVRFEVARRIVERAADHGIPREDVIVDPLVMPIGAMATAGQQVFRLVRRLREELG
                     VNTTCGASNVSFGMPDRERINAAFLPMAIASGLTSAITNPLAAEVKAAIMAADVLMGN
                     DQDAGRWIRAHRDGVAIGTVPTTAIGGATASRPRLNRRALAAAAAMRDAGAAGRNGTA
                     A"
     gene            5678..7789
                     /locus_tag="VIF63_00180"
     CDS             5678..7789
                     /locus_tag="VIF63_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEY8237831.1"
                     /translation="MSAGGGSGAGVNASAGTSAVADAQVIFTPSGRRGRFESGTTILD
                     AARSLGVDLDSVCGGRGLCGRCQVVPAEGSFPKHGIESSDGHLTPRGANEQEYDRLRG
                     LAPGRRLGCFAHIQTDVLVDVPPESQVHRQVVRKGVPVRDFVIDPVVTLHEVELERPD
                     LAKPGGDLTRLLVALETEWGLTGLEADLEVIRDLQPALEEGGYRVTVAVHDRRAITGV
                     WPGLHDRALGVALDVGSTTIAGHLANLTNGEVIASDGVMNPQIRFGEDLMSRVSYAML
                     HEGGAVAMTQAVREAIASLVRGLASKADVAVEEVLELTIVGNPIMHHLLLGIDPVPLG
                     SAPFALATDAAVRVRARDIDLPGHAGARVYVLPCIAGHVGADAAGAILAETPYLADEV
                     TLLVDVGTNAEIVLGSRERLLAASSPTGPAFEGAQISSGQRAAPGAIERVRVDKVTLE
                     PRFRVIGSSRWSDEIGFDASTRRTGVTGVCGSGIVEVLAELFLAGVISADGTIDGRTA
                     ARSPRVIPDGRTFSYVLHEGGPGADLGDGEVGPASPRLLITQNDVRAIQLAKAALYAG
                     ARLLMDHLGVESVDRVKLAGAFGSQIDPLHALILGLVPDAPLDRVAPAGNAAGTGALI
                     ALLSAEARHEIERVVRTVEKIETAVEPRFQEHFVDAMAFPHATAPSTHLALLVDLPKT
                     NDAGDRQVSSARKPNRRPVRA"
     gene            7931..9517
                     /locus_tag="VIF63_00185"
     CDS             7931..9517
                     /locus_tag="VIF63_00185"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY8237832.1"
                     /translation="MIDGPQPGRGGPDDGARGRPGGGRAARHAHRAAVAHAQGVPFLT
                     RLLKPVGILDEEGLSIIERNADTILEEVGIVFRGDPEALESVRLAGADVVGELVKFPR
                     GMCRQIIQATAPKTFTQYARNESRTVQIGDPYTVLAPNYGSPFVRDLDNGRRYGTLED
                     FRNFVKLTYMTPHLHHSGGTVCEPVDIPVNKRHLDMVYSHIKYSDKPFMGSVTHPTRA
                     EDTVELAKIAFSDRGADYLDDHTVILSLINANSPLVWDATMLGVARVYAQANQATLIT
                     PFILAGAMSPVTVAGTAAQTLAEALAGMAFVQLVRPGAPVILGSFASSISMQSGAPTF
                     GTPEPALVLYVMAALARRLGVPFRSGGNLCAAKTADAQAAYESAATFIPTILAGTNFV
                     LHAAGWLEGGLSIGYEKFILDLDQCGMAGVFVKGVDMSENGQALDAIRENGPGLHFLG
                     NSHTLANFETAFYRSETADNNSFEQWLEDGALESPQRANKIWKRMLAEYEPPPLDPGI
                     DEALVEYVTRRKAAFPDSDV"
     gene            9540..10379
                     /locus_tag="VIF63_00190"
     CDS             9540..10379
                     /locus_tag="VIF63_00190"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY8237833.1"
                     /translation="MTADRAALDRVLADPTFELIPLKNVREQAAALPRGATASVTASP
                     GKGIDATVELAIELEGAGMRAIPHLSARMIRDRAHLAELLTRLKDAGIDRAFVVGGDA
                     DEPGEFLDGLSLLRAMADLGQLPAELGCPCYPQGHPDIPEAALAQALRDKAPFVHYMT
                     TQLCFDAKQIATFIAARRTEGITLPVKLGIPGVAEIPKLLSISARIGVKDASKFVLKN
                     ARFVGALLRSGGIYRPSKLMRDLAPLIADPAADVMGLHVYTFNNVAATVEWRDGERAS
                     ARA"
     gene            10537..13290
                     /locus_tag="VIF63_00195"
     CDS             10537..13290
                     /locus_tag="VIF63_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8237834.1"
                     /translation="MRPGAMRPAVLLGLLLVAGGLPVPATVSGVEVTSTVAPGFQETI
                     AISGLSNPTNVRFAADGRVFVAEKRGRIKVYDDLSDTTPTSFSDLTTNVHDFWDRGLL
                     GMTLDPSLTGGVGTGPYVYVLYAYDHILGEVAAAPHWQPEDSCPTPPGSMSDGCVISG
                     RLSRFEVSGSTIMAGEQVLVEDWCQQYPSHSLGGLQFGSDGSLYASAGEGAGFALTDY
                     GQLGGKKVPIVTERNPCGDPPGGIGGAMTAPTAEGGSLRSQDIRGGSDSVGLSGSIIR
                     INPETGAGMPGNPFASSTAENARRIIAYGLRNPFRFTMRPGTNELWLGDVGWNTWEEI
                     NRIPNATDNVAENFGWPCYEGAPRQSAWDAANLNLCEDLYDAGPGAVSAPWFGYDREH
                     PLVPNESCPVGTASVTGLAFYPTTAGSYPAGYAGGLFFADHTRNCIWFMPKGSNGQPD
                     PATAQVFVSDAAHPVDLVIGPGGDLFYVDFEGGTVRRVTATGGNIPPTAVLNASPLTG
                     PTPLTVHFNSTGSFDAEATTLKFAWDLDGDGQLDDATSAAPQWTYTQAANVTVQLRVT
                     DGANLSTTVTKTITVGHTPPVPVISTPTVGTTWKVGDPIAFSGSATDLQDGALPASAL
                     SWSLVMQHCPSICHEHTIQSWTGVAAGSFSAPDHEYPSYLELTLTATDSLGTQASVTR
                     RLDPRTVTLTLASNPSTFSLAFDGGTAAATFTRTVIIGSANSVSAIAPQSYKGATWTF
                     GYWSDGGAASHTIIAPATATTYTAKFRTTSTFFTPIADAQVRPSSPSRNYGHDAVLRV
                     RLNKSRVYLKFTVTGLTGAPASAKLRLWVTDGGPAGGNIYTVSNSWTETGITWNNRPL
                     ISGTKKSTLGAVSTGTWVEFPLGNAITGNGTFSFAISGGTDNVVDYASRETSNDPYLV
                     VTP"
     gene            13326..13991
                     /locus_tag="VIF63_00200"
     CDS             13326..13991
                     /locus_tag="VIF63_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237835.1"
                     /translation="MASCYGFLASISARIGADWASQPGVEEGAQPMTEQISEHSALIE
                     RAPRRRGLRRFAVVFAVFGLLTLAIGPALAHHSNRDVVGASNQFVPNFDGKAGLIGAA
                     DLDESEDEAENEDATDENEDATDEDATDESENEDATDENDQGEDEDAQGEDEDADEDA
                     DEDDGDEDANEDANDDDEDANEDDGDDDEDADEDDGDHDEADDDGGSGGGSDEDDDEE
                     DDD"
     gene            14077..16710
                     /locus_tag="VIF63_00205"
     CDS             14077..16710
                     /locus_tag="VIF63_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8237836.1"
                     /translation="MALPTGPAVTFLFTDIEGSTRLEQSVGTVAWSTVVARHDELLRQ
                     SIESAGGVVVKTEGDSFFAAFSEPAAAVEAAAAAQRAIAASDWPETCQVKVRMGLHLG
                     EGRLRQGIADGEPGDYVGIDVNYTARIAAAGNGGQIVLSQALVDALGGANGGEITDEG
                     LRAVKDFEDPRRLHRLVVHGAADDSRPLRTLEPPSNLPGDVTDLVGREDEIERLSAIL
                     EANRVVTLTGPGGSGKTRLALGVAQRVKDRFPHGTWFIDLAATTNADQLEPATAMTIG
                     LRESPERTFAGALREHLRERQALLVLDNLEQLLPPGAEVVASVARGAPSVRLLVTSRE
                     LLRISGERGHPVPPLDADAGVALFTARATALRPDMALDDEVRAVIRQICDRLFGLPLA
                     IELAAARVRLLSPALILDRLGTSLDLAGGSRDQPERQRTLRGAIDWSHDLLSEPERRL
                     FRRIGVFAGGWTAELALQVVDPDGSLGVDILDGLESLADKSLVRIEVADAAQGAAAEP
                     RFDLHPLLREYALERLDAAGERPDLEARHAAAVAALTQASGPKILGPQGQPTIRRLDR
                     EQHNVRAAIDWALRTGDLETGLRIAAPIWRWFQQRGRLREGRETLDRLLTTAPSDARL
                     HIDALAAVGGLAYWGDDFEASGKAYEQRLALAEQDGDPVLLAEGHYDLGFISMVGSDP
                     EGLRAHESKALELFTEAGTAPGARQGIPKARQALVLGVFLTGDYAGALELEGQNLAEF
                     RAAGSEYQIADSMTFHSGVYLKVGDPAKSWEYVGEGLRWFAANDNQSGIARALGMAAI
                     VLLLHGDADLGTRATGATYRIVEEKGVMLAPVKVLHMPEPKGLAIERLGEERARELLA
                     IGADTPVAQIIAEVLAAPGPR"
     gene            16878..17330
                     /locus_tag="VIF63_00210"
                     /pseudo
     CDS             16878..17330
                     /locus_tag="VIF63_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016098981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
     gene            17327..18268
                     /gene="sufC"
                     /locus_tag="VIF63_00215"
     CDS             17327..18268
                     /gene="sufC"
                     /locus_tag="VIF63_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY8237837.1"
                     /translation="MTDQTNGASHISGPDDRLVIRGLRVAPAGAPEREILQGIDLEVG
                     PGEVHAIMGPNGSGKTTLAYALMGHPSYIVNGGSVTWRGRDILKLSPDKRSRLGMFLA
                     FQYPTAIPGLSVASFIRSALNAKLQGIDKNPDIDPTDAIKGGVSMMDFRKKMRAKLAM
                     LRMDEAFAARYVNDGFSGGEKKRLEMLQMAMLEPEMAILDETDSGLDIDALRIVAEGV
                     NAMLNPNLGVLLITHYQRLLNYITPDVVHVLAQGRIVKSGGKDLALRLEAEGYGPILR
                     EAGLAPDVPDEALLVPQGPDLGEPEAEHAGAKVEAAR"
     gene            18342..19583
                     /locus_tag="VIF63_00220"
     CDS             18342..19583
                     /locus_tag="VIF63_00220"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY8237838.1"
                     /translation="MRDGRLDPEVVRKDFPILATESHGHPLVFLDSASTSQKPLFVID
                     ALDAYYREYTANVHRGIYEIGERATASYEAARASVAKFINAPDSHEVIFTRNATEAIN
                     LVAYSWGRRNIDRGDAIVLTEMEHHANLVPWQLLAQERDGDLEFIPITDDGILRQDVF
                     EVLLRLKPKLVAFTHVSNTLGTINPVREMVDKAHAAGALVLIDGAQAVPHVPVDVQAL
                     GADFYAFSGHKMLAPTGSGARWARRELLEAMPPFMAGGDMIREVHLRRSEWNEIPWKF
                     EAGTPDIAAGIGLGAASEYLLELGMDRVREHERELLVYAFDALGRAHPDLELYGPLNP
                     DLRGGVIPFNVPGVHPHDVAQVLDRSGVAVRAGHHCTMPLHERLELAASARASFNVYT
                     TEADIDALVEGLAEVKRIFGA"
     gene            19717..21213
                     /locus_tag="VIF63_00225"
     CDS             19717..21213
                     /locus_tag="VIF63_00225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8237839.1"
                     /translation="MALALRRRSILVVLAIVALAASLGSVYVLNQPGDNVLPDVPEFP
                     RGGPDSANDNPEKEEQSEKAEEKREAFEEAVEQGKAGTPQTIVLAAAPGWAGESIMDS
                     ATDDWEPAIAADPSAPWVYMLATRYGSKPCPGNCPDPWTALEISSDGGATWSAGVPLC
                     ACKGGGQFDPIIEVVKNTGAVYAVYMNGFNIMFIKSTNHGSTWSAPVKVYGNVSWNDK
                     PVLAVSDNGNDVYVPFNGPTGGDPWLAQSHNGGSTWTQTKLVDSNRYYFTFDADVAPN
                     GTVYLGQSAILYGGGGNKGTTPTGTIDEHVFVSTNSGSTWVDRLVAQVQPGLACTAVG
                     CTPDYYLGHHALAVDNSNNVVFLYDGATAAGGLQTVSAKKSTNGGASWSAAVTLSASG
                     TEAEAPAVESTGNGDVRAWYMQTTGSNADQWNVYYRTSTDGGTTWTAAVDISDATSGA
                     VYKSAAGFQEPYGDYGEIAITSTLKTIAVWGEGASYNGPGGVWFNRQP"
     gene            21242..>22158
                     /locus_tag="VIF63_00230"
     CDS             21242..>22158
                     /locus_tag="VIF63_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237840.1"
                     /translation="MTDAVDRLLSAAEAMGIEIDREEADRWIEAVSSDSGGSITVDVD
                     TGVYGHRVSMADYDHTDIERFRRLALIVGLEDRPPEVMTALALSGSAAQARVHRFPAD
                     ADFFERIHIRAETREAACLLLAAVLRDKALATMRGQGHRLQEVKFGNWPADGSIDGAP
                     VRRGTPVSWTPAQVETGGIAYLTANGSEAVLWWDDAAMDPGWCKLDWVIAVPGEHLLA
                     NVSNMFDATWEAPDGAIIPLDGFLDTYFQEVYLETDSIPLFSKLVKEMGADSVAQYVE
                     QLRGEVYAYTVEHPNYGKAARRLYNIFRLT"
BASE COUNT         3542 a         7963 c         7522 g         3131 t
ORIGIN      
        1 gatcgggcag accggcctgc gcatcgcgcc ggacctgtac atcgcgtgcg gcatcagcgg
       61 cgcgatccag cacatcgtcg gctgtaaggc ggcgaagcgg atcctcgcca tcaacaccga
      121 cccggacgcg ccgatcatgg ccgtcgccga ctacgcggtc atcggcgacc tttccacaat
      181 cgtgcccgcg atcacggccg agatcgagcg ccgccgcgtc ccggcctgag atttctcctc
      241 tgtgtcctgg tacggcttca ggtaatcgac gggcgagagc ccgggtgccc gcaggcgtac
      301 gcgacctggc gctattcggc gagccggcgc ccgacttcgg cgagggcctg ggtgacctcg
      361 tcgtagcgct cgggcgggat cgcgaggact tgtggttcgt gcgcgggatg ctccgggacg
      421 atcaccaggg tcgccggccg tgtgcgttcg atgtcgaact ggattcgacg caggccttcg
      481 aagccgatgt cgagggccac tcggtcgtcg gccgcgatgg cgagccgtcg gtcggtgacg
      541 acgatcttgg cccccaccgc ctcagcgagg actgtgacgg tctccccttc gagcaatcgg
      601 agcgggagtc cgtccaatgg attcgcggaa ggttgggcca tgctgacctc ctggcaggct
      661 cgtgcatcga aggctcgtgc attcgcgtca attgaacccc gttcccggcg aacgatgcgc
      721 agcccgagtc gccctccgcg ctcacgttgg cgttgcatac gcctgagccg cttctgatcc
      781 gctacggccc gcgctgacgc tcagcctccg gcgcgccgca gtcgggccgg gttgagcgcg
      841 atcgcgagcg ggatcatcgc tgcggcgacg cccaccagca cccacagcac gccgacgaag
      901 ttgccggtcg cgtcacgaac caggccgagc acgaccggtg ccgtcgccgc gagggcgtag
      961 ccgatcagca gcatcagcgc cgagatcgat gccgcgtcgt cggcgtcgct tgccacgtcg
     1021 accggcagcg tgagcgcgag cgggaagtac cctccgatgc cgagaccgag gagcgccgtc
     1081 gcggcgtaga tcacgatcga accgggtggc tcggcggacg acagcgcgat gaccagcgcg
     1141 cctccgaccg acagcagccc ggcgatcgtc aactggctcc tccgcgtccc gaagcggtcg
     1201 gcgagcacgg gcacggccag ggtcgcgatg agcccgacgc cggtcagcag cgcgacgagg
     1261 ctgccggcct gcgccgcggt ccagccacgc tcgatctggg cgctcgcgag ccaggtgatg
     1321 cagccgtaga acaggatcga ctgggagccg aagatcagtc cgaggagcca cgccgacggg
     1381 cggcgccagg gcaggcgcgg gagcttcggc atcactcgaa cgtgtcctgc atcccgcggc
     1441 atcagccaga cccacgctgc catcgagacg aaggcggccg atgagacgat cacgaacgcg
     1501 ccgcgccagc cgccgaatgc gtcggcgagc ggaaccgcaa ccgcggccgt gatcgtgccg
     1561 ccgatgacga agccgacgac gtaggcgccg gtgccggcag caggctgctt cggcaggcgc
     1621 gcacggacga tcatcggcag gatcggcccg acgaccgcca tcccgacgcc gatcccgacg
     1681 gtcgcgagca gcaccgtgaa cgtgtccggc agcaggcctc gagccacgcc gaacacgccg
     1741 acgaggagca cgcagatcgt cgccccgagc ttcggcccga tcgatgcggc gagcacgggt
     1801 ccgaacggtg ccagcaggcc catgcacagc accgggatcg tgctcagcag gccggcgatc
     1861 ccgtgcgaca tgccgaggtc cgcctgcacc ttcccgacca gcggcccgat gatcagggcc
     1921 tgtggtcgga gggcggtgcc gacgaggatc agcgccgcga cgacggcgac ccagcggctg
     1981 tcggtcgtct caccagtcgc gttggctgtg gcaggtaaag cttctcctgc gtcgagcggc
     2041 gatgcgccgg tcaagagccg gctcgcaggc gagcaccgat ccgcgccgcc gcagcccgca
     2101 ccgcgctggc gatccggtct tcgtcgcccg gggccgggaa ccggtacgac ggcccgtgga
     2161 cgtgcacggc ggcgacgacc tcggcccgcg cgtcggcgat gggtgcggcg acggagctga
     2221 tgccgacggc gtattcgtcg cgaacccagg cgtggccgtc gcgccggacg tcgcgcattc
     2281 gctcgagcag cgccgacgcg tcggtcaacg tccgagccgt gaacgcctcg agcggttcgg
     2341 cgaggtagcg ggcgagcacc ggcgccgcca gctgggcgag gaagacctgg cccgaagaga
     2401 ccgcgtgcag cgggatccgc gtgccggtcc agtcgcggac cgccacggga ttcgggttcg
     2461 gcggctcgag ctggtccacg tactggaccg tccagccatc ccggatcgag acgccggccg
     2521 cctcgccgag ctcggtcgtc aagtcgacca gggtcggtcg agcgatcgcg acgatgtccc
     2581 aggtctcgcc gagcccggag gcgatcgcca gcaggtcagg cccgagccgg tagcgccgct
     2641 cgccggggac ctgctcgacg gcgccttcgg aggcgagcgc ggcgaggagt cgggcggcgg
     2701 tcgatttcgg gatgcgggac gcgatggcca cttcgctcac gccgagcggt ccatcggcga
     2761 gtgcacggag cacggcgaac gctcgcctga tgctctggac gccgatcgtc gggctggaca
     2821 tgggaccccc gctgggctac cttgacggtc cgattgttcc acaatacggc accgttccgc
     2881 aatgtggaac accggaaccg cgatcttcgc tggttggaac cgtagcggag cgacgcacgt
     2941 ggcggatgga acccaggatt tcgacctctc ggcgctcgcg gatgacgagc tgaccgagca
     3001 gatgcacaac gatctgtatg acggcatggc gccggagatc gtccagggca cgaacatcct
     3061 cctcgagcga ggttggggcc cggacaaggt cctcaacgac gcgctggtcg agggcatgcg
     3121 catcgtcggc atcgacttcc gcgacggcat cctgttcgtg cccgaggtcc tcctcgccgc
     3181 caacgcgatg aaggccggca tggccatcct ccggccgctg ctcgccgaga ccggtgcgaa
     3241 gccgatcggg aaggtcgtca tcggcacggt caagggcgac atccacgaca tcggcaagaa
     3301 cctggtcgcg atgatgctcg agggcgcggg cttcgaggtc tacgaccttg gcatcaatac
     3361 ggacgccgac aagttcatcg ccgcgctcga gctgcacaag cccgacatcc tcggcatgtc
     3421 ggcgctgctc acgacgacga tgccgtacat gaaggtcgtg gtcgccgagc tcgtgaagcg
     3481 cgggatccgc gacgactaca tcgtgctcgt cggcggcgcg ccgctgaacg aggagttcgg
     3541 caaggcggtc ggcgccgatg cctattgccg cgacgccgcg atcgccgtcg agaccgcgcg
     3601 ggccctggtc ctggaacgcc gtgctcgagt cgcctgagct cgatcagcgc gcgacccgtt
     3661 ccgcgatgcg ggacgacatg accgcgcctc gcgcgccact cgtcatcggg tgtggtgcgc
     3721 tcgccgtcga gctcattgcg ctgacccgac gcgcgggcct gcccgcgatg gacctcacgt
     3781 gtctgcccgc gtcgctgcac aaccggcccg agcgcatccc cggcgccgtt gccgcgcgaa
     3841 tccgtcgcgc ccgcgccgac ggctacgacc gcatcttcgt cgcctacgcc gactgcggca
     3901 cgggcggcat gctcgacagg gtgctcgagg ccgagggcgt cgcccgcctc gagggcgccc
     3961 actgctacga ggtctacgcc ggtcgcgccg cgttcgcgac actcgccgac gaggagcccg
     4021 gcaccttcta cctgaccgac ttcctcgtcc gcaacttcga tcgactcgtg atccgcggcc
     4081 tcggcctcga tcgccacccc gagctgctgt cgatctactt cggcaactac cgccggctcg
     4141 tctttctcgc ccagacggac gacgcccagc tgacggcccg cgccgaagcc gccgccgatc
     4201 gactcggtgg cctcacgttc gaacgccgac tggttggcct cggcgagctc gcgacctcga
     4261 ttgcctcgtt cgggaaggcc gcctgatggc gtcgctgatc acgatcctgt ggcgcgacat
     4321 cccgctgcag gtgatcgcgc gcgatcggcg tgaggcgcac aaggtcgtcc tccacccgcg
     4381 gttccaggtt gcggccgaca aggccgcgat gaaggccggc aagcgcgcgg cgagcgacta
     4441 catcgacgag atgcgccgcg acgctcgcga gtgcggcgac gacctcgagg ccgaggcgaa
     4501 cggcgaagcc gagcgactcg aagccgcgta cacgaaggac gtcctggcga cgctgatcgc
     4561 ggccggcggc gtggggcaac ggctgtgatc gccctacgac ctcgcctccc tcgccgctac
     4621 ccggtccgtt atgcgccaat tggcatgaaa tcgccgcgct ggggagacga cgcgtgacaa
     4681 tcaccttggc gagacgctcc atcacccaca ccaccatcag ctccgcgacg cgcgaggtcg
     4741 tgatcgggtt cgaccgaccg ttcacgatca tcggcgagcg catcaatccg accggccgga
     4801 agctgctggc cgaggagatg aaggcgggcg acttcagccg tgtcgagcgc gacgcgatcg
     4861 cccaggtcga ggcgggcgcc cacatgctcg acgtgaacgc gggcattccc ctcgcggacg
     4921 agcccgcgat cctggcgcgg accatccgac tcgtgcagag cctcgtcgac gtgccgctct
     4981 cgatcgactc gtcgatcgtc gaggccctcg aggcggggct cgcggcctac atcggcaagc
     5041 cgctcgtcaa ctccgtcacc ggcgaagagg aacggctcga gcgcgtgctg ccgctgatcc
     5101 ggaagtacgg cgcggccgtc gtcgcgatat cgaacgacga caccggcatc tccgaggacc
     5161 ccgatgtgcg attcgaggtc gcgcgccgca tcgtggaacg cgccgccgac cacggcatcc
     5221 cacgcgagga cgtcatcgtc gacccgctgg tgatgccgat cggcgcgatg gcgaccgcag
     5281 gccagcaggt gttccgcctg gtgagacggc tccgcgagga gctcggcgtc aacacgacgt
     5341 gcggcgcctc gaacgtgagc ttcggcatgc ccgaccgcga gcgcatcaac gcggcattcc
     5401 tgccgatggc gatcgcctcg ggcctgacgt ccgcgatcac gaacccgctc gcggccgagg
     5461 tcaaggccgc gatcatggcc gcggacgtgc tcatgggcaa cgaccaggac gccggtcgct
     5521 ggatccgcgc gcaccgcgac ggcgtcgcga tcggcaccgt cccgacgacg gcgatcggtg
     5581 gcgcgaccgc cagccggccc cgactcaacc gccgggcgtt ggccgctgcc gcggcgatgc
     5641 gcgatgccgg cgcggccggg aggaacggca ccgcggcatg agcgccggag gcggctcggg
     5701 cgcgggcgtg aacgcgagcg cgggcacaag cgccgtcgcg gacgcgcagg tcatcttcac
     5761 gccatcgggg cgccgtgggc ggttcgaatc cgggacgacg atcctcgatg cggcccgctc
     5821 cctcggcgtc gatctcgact cggtgtgcgg cgggcgcggc ctgtgcgggc gctgccaggt
     5881 cgtgccggcc gagggctcgt tcccgaagca cggcatcgaa tcgtcggacg gccacctcac
     5941 cccgcgcggc gcgaacgagc aggagtacga ccgccttcgc ggcctcgcgc ccggccggcg
     6001 cctcggctgc ttcgcccaca tccagacaga cgtgctcgtc gacgtgccgc cggagagcca
     6061 ggtccatcgc caggtcgtgc gcaagggcgt gccggtccgc gacttcgtca tcgatcccgt
     6121 cgtgaccctg cacgaggtcg agctcgaacg gccggacctc gccaagcccg gcggcgacct
     6181 cacccgcctg ctcgtcgcgc tcgaaaccga atggggcctc accgggctcg aggcggacct
     6241 cgaggtcatc cgcgacctcc agcccgcgct cgaggagggc ggctaccgcg tcaccgtggc
     6301 ggtccacgac cgccgcgcga tcacgggcgt ctggcccggc ctccacgatc gcgcgctcgg
     6361 cgtggcgctc gacgtcgggt cgacgacgat cgccggacac ctcgcgaacc tcaccaacgg
     6421 cgaggtcatc gcctcggacg gcgtgatgaa cccgcagatc cgattcggcg aggacctcat
     6481 gagccgcgtg tcgtacgcga tgctccacga gggcggcgcc gtcgcgatga cccaggcggt
     6541 ccgcgaggcg atcgcgtcgc tcgtccgcgg gctcgcgtcg aaggccgatg tcgcggtcga
     6601 ggaggtcctc gagctcacga tcgtgggcaa tccgatcatg caccacctgc tgctcggcat
     6661 cgatcccgtg cccctcggct cggcgccgtt cgcgctcgcg accgacgctg cggtccgggt
     6721 ccgcgcgcgg gacatcgacc tgccgggtca cgcgggcgcg cgcgtctacg tgctgccgtg
     6781 catcgccggc cacgtcggtg cggacgcggc cggggcgatc ctggccgaga cgccctatct
     6841 cgcggacgag gtcacgctcc tcgtcgacgt gggcacgaat gcggagatcg tgctgggatc
     6901 gagggagcgg ctgctcgccg cctcgtcgcc caccgggccc gcgttcgagg gcgcacagat
     6961 ctcgagcggc cagcgcgctg cgcccggcgc gatcgagcgg gtccgggtcg acaaggtcac
     7021 cctcgagcct cggttccggg tcatcggctc gagccgctgg tcggacgaga tcgggttcga
     7081 cgcctccact cgtcgcaccg gcgtgaccgg cgtctgcggc tcgggcatcg tcgaggtgct
     7141 cgccgagctc ttcctcgccg gcgtgatcag cgccgacggc accatcgacg gccggacggc
     7201 agcgcgctcg ccgcgtgtca tcccggacgg ccggacgttc tcgtatgtcc tgcacgaagg
     7261 cgggccggga gcggatctcg gcgacggcga ggtcgggccg gcgtcgccgc gcctcctgat
     7321 cacccagaac gacgtccgcg cgatccagct cgcgaaggcc gcgctctacg cgggcgcgag
     7381 gctcctgatg gaccacctcg gcgtcgagtc ggtcgatcgc gtcaagctcg ccggcgcgtt
     7441 cggcagccag atcgacccgc tgcacgcgct gatcctcggg ctcgtgcccg acgcgccgct
     7501 cgaccgcgtg gcgcccgccg ggaacgccgc gggcaccggc gcgctcatcg cgctgctctc
     7561 ggcagaggcg cgccacgaga tcgagcgcgt cgttcggacc gtggagaaga tcgagaccgc
     7621 ggtcgagccg cgcttccagg agcacttcgt cgacgcgatg gccttcccgc acgccacggc
     7681 cccatcgacc cacctcgccc tccttgtcga cctgccgaag accaatgacg ccggtgatag
     7741 gcaggtctcg agcgctcgga agcccaatag gcgcccggtg cgagcatgat tcgagcgcgg
     7801 cgcacgctca ccgaagcatc gtggaccgcg ctgcgagcgt tcagcccccg ggccgcacgc
     7861 ctgccaccac gctggttggt agatggcgag cgagaaacgg gccgaccaac ttgaacaatg
     7921 gagggagcga atgatcgacg gcccgcagcc tggccgcggc ggtccggacg acggcgctcg
     7981 tggccggccc ggcggcggac gcgcagcgcg ccacgcgcac cgagcggccg tcgcccacgc
     8041 ccagggcgtc ccattcctga cccggttgct caagccggtc gggatcctcg acgaggaagg
     8101 cctctcgatc atcgagcgca acgcggacac gatcctcgag gaggtcggga tcgtcttccg
     8161 gggcgacccc gaggcgctcg agtcggtccg cctcgccggc gccgacgtgg tcggagagct
     8221 cgtcaagttc ccgcgcggca tgtgccgtca gatcattcag gcgacggccc cgaagacctt
     8281 cacccagtac gcgcgcaacg agtcccggac ggtccagatc ggcgatccgt acaccgtgct
     8341 ggcgcccaac tacggcagcc cattcgtgcg cgacctcgac aacggccggc gctacggcac
     8401 gctcgaggac ttccgcaact tcgtgaagct cacgtacatg acgccgcacc tccaccactc
     8461 gggcgggacg gtgtgcgagc cggtcgacat cccggtcaac aagcggcacc tcgacatggt
     8521 ctattcgcac atcaagtaca gcgacaagcc gttcatgggc tcggtgacgc acccgacgcg
     8581 cgcggaggac acggtcgagc tggcgaagat cgcgttcagc gatcgcggcg ccgactacct
     8641 cgacgaccac acggtgatcc tcagcctgat caacgcgaac tcgccgctcg tgtgggacgc
     8701 gacgatgctt ggcgtcgcac gggtctacgc gcaggcgaac caggcgacgc tcatcacgcc
     8761 gttcatcctc gccggcgcga tgtcgccggt gaccgtcgcg ggcaccgcgg cgcagaccct
     8821 cgccgaggcg ctcgcgggca tggccttcgt ccagctcgtg cgaccgggcg cgccggtcat
     8881 cctgggatcg ttcgcctcgt ccatttccat gcagtcgggc gcgccgacgt tcggcacgcc
     8941 cgagccggcc ctggtcctgt acgtcatggc cgcgctcgcg cgccggctcg gcgtgccgtt
     9001 ccgctcgggc ggcaacctat gcgccgcgaa gaccgccgac gcccaggccg cgtacgagtc
     9061 cgccgcgacc ttcatcccga cgatcctcgc gggcacgaac ttcgtgctcc acgccgcggg
     9121 ctggctcgag ggtgggctgt cgatcggcta tgagaagttc atcctcgacc tcgaccagtg
     9181 cggcatggcc ggcgtgttcg tgaagggcgt cgacatgtcc gagaacggcc aggcgctcga
     9241 cgcgatccgc gagaacggcc cgggcctcca cttcctcggc aactcgcaca ccctcgccaa
     9301 cttcgagacc gcgttctacc gctccgagac cgcagacaac aactcgttcg agcagtggct
     9361 cgaggacggc gcgctcgaat cgccccagcg tgcgaacaag atctggaagc ggatgctcgc
     9421 ggagtacgaa ccgccgccgc tcgacccggg catcgacgaa gcgctcgtcg agtacgtcac
     9481 tcggcgcaag gccgccttcc ccgactcgga cgtctagcga ccgccctccg atgcccgcca
     9541 tgaccgcgga tcgcgctgcc ctcgatcgcg tcctcgctga tccgaccttc gagctgatcc
     9601 cgctcaagaa cgtgcgcgag caggctgcgg ccctgccgcg aggcgccacg gcgtcggtga
     9661 cggcgtcacc cggcaagggg atcgacgcga ccgtcgagct ggcgatcgag ctcgaggggg
     9721 ccggcatgcg cgcgatcccg cacctctcgg cgcggatgat ccgcgaccgc gcgcacctgg
     9781 ccgagcttct cacccgcctc aaggacgcgg gcatcgatcg ggcgttcgtc gtgggcggtg
     9841 acgcggacga gcccggggag ttcctcgacg gcctctcgct gctccgagcc atggccgacc
     9901 tcggccaact ccccgcggag ctcggctgcc cgtgctatcc gcagggtcac cccgacatcc
     9961 ccgaggcggc cctcgcccag gcgctgcgcg acaaggcgcc gttcgtgcac tacatgacca
    10021 cccagctctg cttcgacgcg aaacagatcg ccaccttcat cgccgcccgc cgcaccgagg
    10081 gaatcaccct gccggtgaag ctcggcatcc ctggcgtcgc cgagatcccg aagctgctgt
    10141 cgatcagtgc ccgcatcggc gtgaaggacg cgagcaagtt cgtgctcaag aacgcacggt
    10201 tcgtgggcgc gctcctccgc tccggcggca tctaccgccc ctcgaagctg atgcgcgacc
    10261 ttgcgccgct catcgccgac cccgccgccg atgtcatggg tctccacgtc tacaccttca
    10321 acaacgtcgc cgccacggtc gaatggcgcg atggcgagcg agcatccgcc cgcgcctgag
    10381 gcgcgcagga caacggtgcc gggtgtcacc gaatgggccg aatcgggcat gaccatcggc
    10441 tagagttggc cgctggagga tcgagcagcc cccggtgcga agcgccgccc ggtcgatcct
    10501 cgaatggggg agccgattcg gatggggagg cctcgattgc ggccaggcgc gatgcgcccg
    10561 gcagtcctgc tcggcctcct gttggttgcc ggtggactcc ccgtccccgc gacggtctcg
    10621 ggcgtcgagg tcacgtcgac cgtcgctccg ggcttccagg agacgatcgc gatcagcggc
    10681 ctctcgaacc cgacgaacgt ccggttcgcc gcggacggtc gcgtcttcgt cgccgagaag
    10741 cgcgggcgga tcaaggtcta cgacgacctt tccgacacga cccctacgtc gttcagcgac
    10801 ctcacgacga acgtccacga cttctgggat cggggcctgc tcggcatgac cctcgacccg
    10861 agcctcaccg gcggggtcgg caccggaccg tacgtgtacg tcctgtacgc ctacgaccac
    10921 atcctcggtg aggtcgcggc cgcgccgcac tggcagcctg aggacagctg tccgacgcct
    10981 cccggctcga tgtccgatgg ctgcgtcatc agcgggcgcc tctcgcgatt cgaggtcagc
    11041 gggtccacga tcatggccgg ggagcaggtc ctggtcgagg actggtgcca gcaatacccg
    11101 agccatagcc tcggcgggct ccagttcgga tcggacggct cgctgtatgc cagcgccggc
    11161 gagggcgctg ggttcgccct caccgactac gggcagctcg gcgggaagaa ggttccgatc
    11221 gtcacagagc gcaacccgtg cggcgaccca ccgggcggga tcggtggcgc gatgaccgca
    11281 cccacggccg agggcgggtc cctccgaagc caggacatcc gcggcggcag cgattccgtc
    11341 ggcctgagcg ggtcgatcat ccgaatcaat ccggaaaccg gcgccggcat gcccggcaac
    11401 ccattcgcct cgagcaccgc tgagaacgcc cgccggatca tcgcctacgg cctccggaac
    11461 ccgttccggt tcacgatgcg ccccggcacg aacgagctct ggctcggcga cgtcggctgg
    11521 aacacctggg aggagatcaa ccggatcccg aacgcgacgg acaacgtcgc cgagaacttc
    11581 ggttggccgt gctacgaggg cgcgccccgg caatcggcat gggacgccgc caacctcaac
    11641 ctgtgcgagg acctgtacga cgcgggcccg ggcgccgtct cggcgccatg gttcgggtac
    11701 gaccgggagc atcccctggt cccgaacgag agctgccccg tcggcaccgc ctcggtgacc
    11761 ggcctggcct tctacccgac gaccgccggc tcgtacccgg cggggtacgc cggcggcctg
    11821 ttcttcgccg atcacacgcg caactgcatc tggttcatgc ccaagggctc gaacggccag
    11881 cccgatcctg caaccgccca ggtgttcgtc tccgacgccg cgcatccggt cgatctcgtg
    11941 atcggtcccg gcggcgacct gttctatgtc gatttcgaag gcgggaccgt ccgccgagtc
    12001 accgcgaccg gtggcaacat tccgccgacg gcggtcctga acgcgagccc gctgaccggg
    12061 cccacgccgt tgaccgtgca cttcaactcg acggggtcgt tcgacgcgga ggcgacgacg
    12121 ctgaagtttg cgtgggacct cgacggcgat ggccagctcg acgatgccac ctccgccgcg
    12181 ccccagtgga catacaccca ggcggccaac gtcaccgttc aacttcgggt gaccgatggg
    12241 gccaacctct cgacgacggt gacgaagacg atcaccgtcg gccacacgcc accggtgccg
    12301 gtcatctcga cgccgacggt cgggacgacc tggaaggttg gcgacccgat cgcgttcagc
    12361 ggctccgcca ccgatctgca ggacggcgcc ctgcccgcgt ccgccctttc gtggtcgctc
    12421 gtcatgcagc attgcccgtc gatctgccac gagcacacga tccagagctg gacgggcgtc
    12481 gccgcgggat cattcagcgc ccccgatcac gagtacccct cgtacctcga gctcacattg
    12541 accgcaacgg actcgctggg gacccaggcg tcggtgacga ggcgcctcga tcccaggacg
    12601 gtgacgctca cgctcgcgag caacccgagc acgttctcgc tcgccttcga cggcggcacg
    12661 gcggcggcga ccttcactcg gacggtgatc atcgggtcgg cgaactcggt cagcgcgatc
    12721 gccccgcagt catacaaggg cgcgacctgg acgttcggct actggtcgga cggcggggcc
    12781 gcctcccaca cgatcatcgc gcccgcgacc gccaccacct acaccgccaa gttcaggacg
    12841 acatcgacct tcttcacgcc aatcgccgac gcccaggttc ggccgtcgtc gccgtcgagg
    12901 aactacgggc acgacgccgt cctgcgggtc cgcctgaaca agtcccgggt ctacctcaag
    12961 ttcacggtca cggggctcac gggggcgccg gcctccgcga agcttcggct ctgggtgacc
    13021 gacggggggc cggccggcgg aaacatctac acggtgtcga attcctggac cgagaccggg
    13081 atcacctgga acaaccgacc cctgatctcc ggcaccaaga agtcgacgct cggcgcggtc
    13141 tcgacgggga cctgggtgga gttcccgctc ggcaacgcga tcaccggcaa cggcacgttc
    13201 agcttcgcga tctcgggcgg caccgacaac gtcgtcgact acgccagccg cgagaccagc
    13261 aacgacccgt acctcgtggt cacgccctga gccgaggcgg tgcggggcgg cactggcccg
    13321 aaagggtggc gtcttgttac ggatttctcg cgtccatttc cgcccgtatc ggggcagact
    13381 gggcttctca accgggggtt gaagaaggag cccaaccaat gaccgagcaa atctcagagc
    13441 acagcgcact catcgagcgc gccccacggc gacgcggcct ccgacggttc gcggtcgtgt
    13501 ttgctgtctt cggcctcttg acgctggcca tcgggccggc tcttgcccac cacagcaaca
    13561 gggacgtggt cggcgccagc aaccagttcg tgccgaactt cgacggaaag gccggcctca
    13621 tcggggccgc cgatctcgac gagtccgagg acgaagccga gaacgaggac gcgaccgacg
    13681 agaacgagga cgcgaccgat gaggacgcga ccgacgagag cgagaacgag gacgcgaccg
    13741 acgagaacga ccagggcgag gacgaggacg cccagggcga ggacgaagac gccgacgagg
    13801 acgccgacga agatgacggc gacgaagacg ccaacgaaga cgccaacgac gacgacgaag
    13861 acgccaacga ggacgacggc gacgacgacg aggatgccga cgaagacgat ggcgaccacg
    13921 acgaagccga tgacgacggc gggtcgggtg gcggcagcga cgaggatgac gacgaggaag
    13981 acgacgactg agctcgtgag ctgacaccaa cccgctggaa gggcaccggg atcgacccgg
    14041 tgccctttcg attccgtgcc aacatggcaa ccccagatgg cactgccgac cggacccgcc
    14101 gtcacgttcc tgttcacgga catcgagggc tcgacgcgcc tcgagcagtc ggtcggtacc
    14161 gtggcctggt cgaccgtcgt cgcgcgccac gacgagcttc tccgccagtc gatcgagtcg
    14221 gccggaggcg tcgtcgtcaa gaccgagggc gattccttct tcgccgcgtt ctcggagccc
    14281 gcggccgctg tcgaggcagc tgccgcggcc cagcgagcga tcgccgcgag tgactggccc
    14341 gagacgtgcc aggtcaaggt gcggatgggc ctccacctgg gcgagggccg cctccggcag
    14401 ggcatcgccg acggcgagcc gggcgactac gtcgggatcg acgtcaacta caccgcccgg
    14461 atcgcggcgg ccggcaacgg tggccagatc gtgctgtcgc aggcgctggt cgatgcgctt
    14521 ggtggcgcca acggaggaga gatcaccgac gagggcctgc gcgcggtcaa ggacttcgag
    14581 gatccaaggc gactgcatcg attggtggtg cacggcgcgg ccgacgactc gcgtccactc
    14641 cggacgctcg agccgccgag caacctgccc ggtgacgtca ccgacctggt cggtcgtgag
    14701 gacgagatcg agcgcctgag cgcgatcctc gaggccaacc gcgtcgtgac cctgaccggc
    14761 ccgggcggca gcggcaagac gcgcctcgcg ctcggcgtcg cccagcgggt gaaggaccgg
    14821 ttcccgcacg gaacctggtt catcgacctc gccgcgacga cgaatgcgga ccagctcgaa
    14881 ccggcaacgg cgatgacgat cggcctgcgc gagtcacccg agcgcacgtt tgcgggggcc
    14941 ctccgcgagc atctccgcga gcgccaggcg ctgctcgtgc tcgacaacct cgagcagctc
    15001 ctcccgcccg gcgccgaggt cgtggcctcg gtcgcgcgcg gcgcgccatc ggtgcgactc
    15061 ctggtcacga gccgcgagct gcttcggatc tcgggcgagc gcgggcatcc ggtcccgccg
    15121 ctcgacgccg atgccggcgt ggcgctgttc acggcgcgcg cgacggcgct gcgcccggac
    15181 atggcactcg acgacgaggt cagggcagtg atccgccaga tctgtgaccg gcttttcggg
    15241 ctgccgctcg cgatcgagct cgctgcggct cgggtacgcc tgctgagccc ggcgctgatc
    15301 ctcgaccggc tcggaaccag ccttgatctc gcaggcgggt cacgcgacca gcccgagcgc
    15361 cagcggaccc tgcgcggcgc gatcgattgg agccacgacc tgctgtccga gccggagcgc
    15421 cggctcttcc gtcgaatcgg ggtcttcgcc ggcggctgga ccgccgagct cgcgctgcag
    15481 gtcgtcgatc ccgacggctc gctcggcgtc gacatcctgg acgggctcga atcgctggcc
    15541 gacaagagcc tcgtccggat cgaggtcgcc gacgcggcgc agggcgccgc cgcggagccc
    15601 cgcttcgacc tccatccgct gctccgcgag tacgccctcg agcggctcga cgcggcgggc
    15661 gagcggcccg acctcgaggc ccgccacgcg gccgcggtcg ccgcgctcac ccaggcctcc
    15721 ggcccgaaga tcctgggacc gcagggccag ccgacgatcc gacgactcga ccgcgaacag
    15781 cacaacgttc gggccgccat cgactgggcg ctgcgaaccg gggatctcga aaccggcctc
    15841 cgtatcgccg caccgatctg gcgctggttc cagcagcgcg gccgccttcg cgagggccgc
    15901 gagaccctcg atcggctgct tacgaccgcg ccctcggacg cccggctgca catcgacgcg
    15961 ctggcggccg tcggtggatt ggcgtactgg ggcgacgact tcgaggcgtc aggcaaggcc
    16021 tacgagcagc ggcttgccct ggccgagcag gacggcgatc cggtgctcct ggcggagggc
    16081 cattacgacc tcgggttcat ctcgatggtc ggcagcgacc cggagggcct ccgagcgcac
    16141 gagtcgaagg ctctcgagct gttcaccgag gccgggaccg cgcccggcgc caggcaaggg
    16201 atcccgaaag cgcgccaggc gctggtgctt ggcgtgttcc tgaccggcga ttacgccggc
    16261 gccctcgagc tcgagggcca gaacctggcg gagttccgcg cagccgggtc cgagtaccag
    16321 atcgccgaca gcatgacctt ccactccggg gtctatctca aggtcggcga cccggccaaa
    16381 tcatgggagt acgtcggcga gggtctccgc tggttcgccg ccaacgacaa ccagagcggc
    16441 atcgcccgcg cgctggggat ggccgccatc gtgctcctgc tgcatggtga cgccgacctc
    16501 gggacgcgcg caaccggcgc gacctaccgg atcgtggagg agaagggcgt gatgctcgca
    16561 cccgtgaagg tccttcacat gcccgaaccc aagggcctcg ccatcgagcg cctcggcgag
    16621 gagcgagccc gcgagctgct cgcaatcggc gctgacacgc cggtcgccca gatcatcgcg
    16681 gaggtcctcg ccgcacccgg tcctcgctga gctgcaactc ggcgacgccc cgccggctga
    16741 cggtttgacg ctccgggggg ccactcgtag actcaatccc gacaagatcg cttgggattg
    16801 aggaccgtct cctgacctcc accgagaccg ccccggacgt cgcgccgcac cgcccgtcat
    16861 tccacggaac gggtgccgtc gcgttttcca ccaagggcga atacggcgtt cggttgatgg
    16921 tccagctcgg ccggcatttc ggcgccggac catcgtccct ggccgagatc gccgcggccg
    16981 aggacatgcc tcgcgcctat ctcgagcagc tcgcgttcgt gctgcgcgac gcagggctgg
    17041 tggtgtccac gcggggcgcc cacggcggct acgagcttgc gcgcccgccg gcttcgatcc
    17101 cgatgagcga ggtgctccga gccctcgaag gtcctctcgc gccgatgatc tgcgcaaccg
    17161 aggacgcgcc gggccacgac ctcgcctgcg atcgcacggc ctcgtgcacc gtgaacctgc
    17221 tctggatccg cgttcgggac gccgtcgccg gcgcactcga tggcgtgacc ctcgccgacc
    17281 tcgtgccaca gcgcgtggtg cccctccaag gagcgttgac cgcatcgtga ccgaccagac
    17341 gaacggcgcc tcgcacatct ccgggccgga cgaccggctc gtcatccgcg gcctgcgcgt
    17401 cgcgcccgcc ggtgcgcccg agcgcgagat cctgcagggc atcgacctcg aggtcgggcc
    17461 gggcgaggtc cacgcgatca tgggcccgaa cggctcgggc aagacgaccc tcgcctacgc
    17521 gctgatgggc cacccctcgt acatcgtgaa cggcggctcg gtgacgtggc gcggccgcga
    17581 catcctgaag ctgagcccgg acaagcgctc gcggctgggc atgttcctcg ccttccagta
    17641 cccgacggcg atccccggcc tgtcggtcgc gagcttcatc cggagcgcgc tcaacgccaa
    17701 gctccagggc atcgacaaga accccgacat cgacccgacc gacgccatca agggcggcgt
    17761 ctcgatgatg gatttccgca agaagatgcg cgccaagctc gcgatgcttc ggatggacga
    17821 ggcgttcgcc gcgcgctacg tcaacgacgg cttctcgggc ggcgagaaga agcgcctcga
    17881 gatgctccag atggcgatgc tcgagcccga gatggcgatc ctcgacgaga cggactcggg
    17941 gctcgacatc gatgcgctgc ggatcgtggc cgagggcgtc aacgcgatgc tcaacccgaa
    18001 cctcggcgtg ctgctgatca cgcactacca gcggctgctc aactacatca cccccgacgt
    18061 cgtccacgtg ctggcgcagg gccggatcgt caagagcggc ggcaaggacc tcgccctccg
    18121 gctcgaggcc gagggctacg gcccgatcct gcgcgaggcc ggcctggcgc ccgatgtccc
    18181 cgacgaggcc ctgctcgtgc cccagggccc ggacctcggc gaaccagagg ccgagcacgc
    18241 cggcgccaag gtggaggccg cccgctaggc gggcgatcga cccgatgacc gtcaccgtgc
    18301 cgcgccccgc cgccgagccc gccctcgggt tcggcgggcg gatccgggac ggtcgccttg
    18361 atcccgaggt ggttcgcaag gacttcccga ttctcgccac cgagtcgcac ggccacccgc
    18421 tcgtcttcct cgactccgca tcgacgagcc agaagccgct gttcgtcatc gatgccctcg
    18481 acgcctacta ccgcgaatac accgcgaacg tgcaccgcgg catctacgag atcggcgagc
    18541 gggcgaccgc gagctacgag gcggctcgcg cgtccgtggc gaagttcatc aacgccccgg
    18601 acagccacga ggtcatcttc actcgcaacg cgaccgaggc gatcaacctc gtcgcgtact
    18661 cgtggggccg ccgcaacatc gaccgcggcg acgcgatcgt gctcaccgag atggagcacc
    18721 acgcgaacct cgtcccctgg cagctcctcg cccaggagcg cgacggcgac ctcgagttca
    18781 tcccgatcac cgacgacggc atcctccgcc aggacgtctt cgaggtcctg ctccgcctga
    18841 agccgaagct cgtcgcgttc acccacgttt cgaacacgct gggcacgatc aacccggtcc
    18901 gggagatggt cgacaaggca cacgcggccg gcgcgctcgt cctcatcgac ggcgcccagg
    18961 ccgtgcccca cgtcccggtc gacgtccagg cgctcggcgc cgacttctac gcgttctccg
    19021 gccacaagat gcttgccccg accggttccg gcgcgcggtg ggcgcggcgc gagctgctcg
    19081 aagcgatgcc gccgttcatg gccggcggcg acatgatccg cgaggtccac ctccgccggt
    19141 ccgagtggaa cgaaattccc tggaagttcg aggcaggcac gcctgacatc gcggccggca
    19201 tcgggctggg cgcggcatcg gaatacctgc tcgagctggg catggaccgc gtgcgcgagc
    19261 acgagcgcga gctcctcgtc tatgccttcg acgcgctcgg ccgggcacac cccgacctcg
    19321 agctctacgg gccgctgaat ccggacctgc gcggcggcgt gatcccgttc aacgtgccgg
    19381 gcgtccaccc ccacgacgtc gcccaggtgc tggatcgctc cggcgtcgcc gtccgtgccg
    19441 gccaccactg cacgatgccc ctgcacgagc gcctcgagct cgccgccagc gcccgcgcca
    19501 gcttcaacgt ctacacgacc gaggccgata tcgacgcgct cgtcgagggc ctggccgagg
    19561 tgaagcggat cttcggggcc tgaccggcaa ccggtgccca ggcgcaccgg gccgatccca
    19621 agcggcgttt gccgtccagc cgaccggaat acactccgcg gcgcgcggcc ggagcgctgg
    19681 agggcgcagc cccgcgaccg agtctggagt cgtgtcatgg ccctggcact gcgacgccga
    19741 tcgatccttg tcgtcctggc aatcgtcgcc ctcgccgcga gcctgggcag cgtgtacgtc
    19801 ctgaaccagc cgggcgacaa cgtcctgccc gatgtgcccg agttcccgcg cggcggcccg
    19861 gacagcgcga acgacaaccc cgagaaggaa gagcagtcag agaaggccga ggagaagcgt
    19921 gaggccttcg aggaagcggt cgagcagggc aaggccggca ccccgcagac gattgtcctg
    19981 gctgccgcgc ccggttgggc cggtgagtcg atcatggatt cggcgacgga cgactgggag
    20041 ccggcgattg ctgcagatcc cagcgccccg tgggtgtaca tgctggccac gcgctacggc
    20101 tcgaagccct gccccggcaa ctgccccgac ccatggaccg ctctcgagat cagctccgat
    20161 gggggcgcga cgtggtccgc gggcgtgccc ctgtgcgcgt gcaagggcgg tggccagttc
    20221 gacccgatca tcgaggtcgt gaagaacacc ggcgccgtgt acgccgtcta catgaacggc
    20281 ttcaacatca tgttcatcaa gtcgaccaat cacggctcca cgtggtcggc cccggtgaag
    20341 gtctatggca acgtgtcgtg gaacgacaag ccggtcctcg cggtgagcga caacggcaat
    20401 gacgtctacg tgcccttcaa cgggccgacc ggcggcgacc cgtggctcgc ccagtcgcac
    20461 aacggtgggt cgacgtggac gcagacaaag ctcgtcgaca gcaatcgtta ctacttcacg
    20521 ttcgacgccg acgtcgcgcc gaacggcacc gtctacctgg gccagagcgc gatcctgtac
    20581 ggcggcggcg gcaacaaggg cacgacgcca accggcacga tcgacgagca cgtgttcgtc
    20641 tcgacgaaca gcggctcgac atgggtggac aggctcgtgg cccaggtcca gccgggcctc
    20701 gcctgcacgg cggtcggctg cacgcccgac tactacctcg ggcaccatgc gttggcggtc
    20761 gacaacagca acaacgtcgt gttcctgtat gacggggcca ccgctgccgg cgggctgcag
    20821 accgtgtcgg cgaagaagtc caccaacggc ggcgcctcct ggtcagcggc agtcaccctg
    20881 tcggcgagcg gcaccgaagc ggaggccccg gccgtcgaat cgaccgggaa cggcgacgtt
    20941 cgagcgtggt acatgcagac caccgggtcg aacgcggacc aatggaacgt ctactaccga
    21001 acctcaacgg atggcggtac cacgtggacc gcggcggtcg acatcagcga cgccacgagc
    21061 ggggccgtct acaagtcggc ggcgggcttc caggagccct acggcgacta cggcgagatc
    21121 gccatcacct cgaccctgaa gaccatcgcg gtctggggtg aaggcgcgag ctacaacggt
    21181 cccggcggcg tctggttcaa tcggcagccg tagggctgcc gaggaccagt gcgggggagt
    21241 catgacggac gcggtcgatc ggctgctctc agccgcagag gcgatgggca tcgagatcga
    21301 ccgcgaggag gccgatcgct ggatcgaggc ggtctcgtcc gattccggcg gcagcatcac
    21361 ggtcgacgtc gacaccggtg tctacgggca tcgcgtgtcg atggccgact acgaccacac
    21421 cgacatcgag cgcttccggc gcctggcgct gatcgtcggg ctcgaggatc gcccaccgga
    21481 ggtgatgacc gcacttgcgc tctcgggctc agccgcccag gcgcgcgtcc atcggttccc
    21541 ggccgacgcg gacttcttcg agcgcatcca catccgcgcc gagacgcgcg aggcggcgtg
    21601 cctcctgctc gccgcggtcc ttcgcgacaa ggcgctggcg accatgcgcg gccagggcca
    21661 ccggctgcag gaggtcaagt tcggcaactg gccggccgac gggtcgatcg atggcgcgcc
    21721 ggtcaggcgc ggcacgcccg tgtcgtggac cccggcgcag gtcgagaccg gcggcatcgc
    21781 ctacctgacc gccaacggtt ccgaggccgt cctctggtgg gacgacgcgg ccatggaccc
    21841 gggctggtgc aagctcgact gggtcatcgc cgtcccgggt gagcacctcc tcgccaacgt
    21901 ttcgaacatg ttcgatgcga cctgggaggc gcccgacggc gcgatcatcc cgctcgacgg
    21961 cttcctcgac acgtacttcc aggaggtcta cctcgagacg gactcgatcc cgctcttctc
    22021 gaagctggtc aaggagatgg gcgcggattc cgtcgcgcag tacgtggagc agctgcgtgg
    22081 cgaggtctac gcgtacacgg tcgagcatcc gaactatggc aaggccgcgc gccggctcta
    22141 caacattttc cggctcac
//
LOCUS       DATBSW010000005         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_740965, whole genome shotgun sequence.
ACCESSION   DATBSW010000005 DATBSW010000000
VERSION     DATBSW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740965"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VIF63_00235"
     CDS             complement(<1..814)
                     /locus_tag="VIF63_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8237841.1"
                     /translation="MTSEAILGRLMLAFNGTTVPSWLDERLATAPAAGVTLFRQPNLR
                     NPNQVRRLTTALQAAAGRRPGSDGLLLLIAADQEGGQLLALGNGFTPFGSPMAIGATG
                     DAELAERIGRAIGRELRAVGVNVNYAPVLDVAVNPSNPSLGIRSFGDDPAAVARLATA
                     WLRGLQSAGVAGTGKHFPGKGEGNVDTHLALDVVNRDREALEAVELVPFRALIAAGVR
                     LVMSGHFAVPALTGSPTLPSTLSRRVMGTLLRDELGFDGVAITDALDMQALPQ"
     gene            887..2251
                     /locus_tag="VIF63_00240"
     CDS             887..2251
                     /locus_tag="VIF63_00240"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8237842.1"
                     /translation="MTDAALDLDPGALAGRFPAGFGWGFASSAYQIEGAATEDGRGRS
                     VWDTFSRVPGAIADGQTGDIACDHYHRFAEDVGLMAQLGAKTYRFSISWPRVLPEGTG
                     AANEPGLAFYDRLVDALLAAGIKPLVNLFHWDLPQALQDRGGFADPQVVGWFADYATL
                     MAKRLGDRVSDWMTLNEPAVYAFLGHADGIHAPGLQDWPTALRVADNQLKAHAAAVAA
                     IRSARKKARVGIAFDMNQVAPATDTDRDRRAAALWSAARDAWYLDPLFGRGYPKLGLE
                     AHGSAGHLRGVELSDPPPGDLDYLGLNYYRRDRVSAVSDRAFDWMIDAGDGTDQTQMG
                     WEIAPDGLRDVLVALHREYAPRELIVTENGAAYPDSVDADGRVRDDPRVAYLARHIAA
                     AAEALAAGVPLTGYHVWSLLDNYEWSLGYTRRFGVVHVDFETQVRTLKDSADWYGRLI
                     AAAT"
BASE COUNT          359 a          875 c          817 g          329 t
ORIGIN      
        1 cctggggcag cgcctgcatg tcgagcgcgt cggtgatcgc gaccccgtcg aacccgagct
       61 cgtcgcgcag cagcgtgccc atcacccgcc gcgacagggt cgatggcagc gtcgggctgc
      121 cggtcagggc agggaccgcg aaatggcctg acatgacgag ccgcacgcct gccgcgatca
      181 acgcccggaa cggcacgagc tcgaccgcct cgagcgcctc gcgatcccgg ttgaccacgt
      241 cgagggccag gtgggtgtcg acattcccct cgcccttgcc cgggaagtgc ttgccggtgc
      301 ccgcgacccc cgcggactgc agcccgcgga gccaggcggt cgcgagtcgc gcgaccgccg
      361 ccgggtcatc cccgaacgag cgaatcccga gcgacgggtt ggagggattg accgcaacgt
      421 ccagcaccgg cgcgtagttc acgttgaccc cgaccgcgcg cagctcccgc ccgatcgccc
      481 ggccgatacg ctccgcgagc tcggcatcgc ccgtcgcgcc gatcgccatc gggctgccga
      541 acggggtgaa gccgttgccg agcgccagca gctggccgcc ctcctggtcc gccgcgatca
      601 gcagcagcag gccgtcgctg cccgggcggc gaccggccgc cgcctgcagt gcggtcgtca
      661 gccggcggac ctgattcgga ttccgcaggt tcggttgccg gaacagggtg acgcccgccg
      721 cgggtgcggt cgcgaggcgc tcgtcgagcc acgacgggac ggtggtgccg ttgaacgcga
      781 gcatcagccg gcccaggatc gcctcggagg tcatcccccg aagtatcgtc ggtcggcagg
      841 cgcgtctcac gggccgatct cgagcctcgc cgcataccat cgagcaatga cagacgctgc
      901 actcgacctc gacccgggcg cccttgccgg gcgcttccca gcgggcttcg gctggggatt
      961 tgcatcctcc gcgtaccaga tcgaaggcgc agcgaccgag gacggcaggg gtcgctcggt
     1021 ctgggacacg ttctcgcgcg tgccgggcgc catcgccgat ggccagacgg gcgacatcgc
     1081 gtgcgatcac taccaccgct tcgccgaaga cgtggggctg atggcccagc tcggcgcaaa
     1141 gacctaccgg ttcagcatca gctggccccg ggtcctcccc gagggcaccg gcgcagccaa
     1201 cgaacccggg ctcgcgttct acgaccgcct ggtcgatgcc ctcctcgccg ccggcatcaa
     1261 gccgctcgtc aacctgttcc attgggacct gccccaggcc ctccaggatc gcggcggctt
     1321 cgccgacccg caggtcgtcg gctggttcgc cgactacgcg acgctgatgg caaagcgcct
     1381 gggcgatcgc gtctccgact ggatgaccct caacgagccc gccgtgtacg ccttcctcgg
     1441 ccacgccgac gggatccacg ctccgggcct ccaggactgg ccgaccgccc ttcgcgtcgc
     1501 ggacaaccag ctcaaggccc acgccgccgc ggtcgcggcc atccgctcgg ctcggaagaa
     1561 ggcacgggtc gggattgcct tcgacatgaa ccaggtcgcg ccggccaccg acacggaccg
     1621 ggaccggcgc gccgcggccc tatggagcgc tgcccgcgac gcctggtacc tcgatcccct
     1681 gttcgggcgc ggctatccca agctcggtct cgaggcgcac ggctccgccg gacacctgcg
     1741 cggcgtcgag ctgagcgacc caccgccggg cgacctcgac tacctgggcc tgaactacta
     1801 ccggcgtgat cgggtcagcg cggtgtcgga tcgcgcgttc gattggatga tcgatgcggg
     1861 agacggcacg gaccagacgc agatgggctg ggagatcgcg cccgacggcc tgcgcgacgt
     1921 cctggtcgca ctgcatcgcg agtacgcgcc gcgcgagctc atcgtcactg agaacggtgc
     1981 cgcgtacccg gacagcgtcg atgccgacgg ccgggtccgc gacgaccctc gcgtggcgta
     2041 cctggcccgc cacatcgcgg cggccgccga ggcgctggcg gccggcgtgc cgttgacggg
     2101 ctaccacgtg tggtcactgc tcgacaacta cgagtggagc ctcggttaca cgcgccggtt
     2161 cggcgtcgtc cacgtcgatt tcgagaccca ggtccggacg ctgaaggaca gcgccgactg
     2221 gtacgggagg ctgatcgccg cggctacttg acggagccgg ccaggatccc tcgcacgaag
     2281 gctcgctgga gagccaggaa gacgcccagt gggacgacca tcgtcaggaa ggcacccgcc
     2341 gtgaggagat gccacgatcc gccgcgtggt ccgagcatgt
//
LOCUS       DATBSW010000006         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_432, whole genome shotgun sequence.
ACCESSION   DATBSW010000006 DATBSW010000000
VERSION     DATBSW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VIF63_00245"
     CDS             complement(<1..1250)
                     /locus_tag="VIF63_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY8237843.1"
                     /translation="MESLPAFAQPVKGSAGATKPPVRLGIIYFSNGVEPAHWWAKGSG
                     ADMELGPGLEPMLPHREDMVFIRGLFNQSAQVSTSPHLGRMNLLSGAPVSLDPNQIRV
                     GTSMDQVLATAFADETEVPSLVLGIEPNELRLEDGLSMIYGSSLSWISPTRAAAKEIY
                     PARTFDRLVDDGSGRTLDRSILDAVWDESQGLRPRISKDDDHKLSEYFEGIRDIEKRI
                     ERASTEERLEGWRPTLAQPDMPRPKHELPQDVPAHMKLMLDLIVLAFQMDKTRVATLM
                     LNNDLSQMNFKFLDGVQGALHLDLTHNGRAADKEAMYLKTNQFHIEQFAYLTQRMKDI
                     AEGETTLLDNSILMMASNLFDGDLHSANQLPVLLTGKGGGSLATGRILDYLDKGDEHR
                     KACSLHLSLMDRMGVRLDRFGDSTT"
     gene            complement(1328..>2057)
                     /locus_tag="VIF63_00250"
     CDS             complement(1328..>2057)
                     /locus_tag="VIF63_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEY8237844.1"
                     /translation="FYGIDAGVSSNDRVERVEGARAFDRGGALRLGSVLTTTSAPLRT
                     SPVKRGDWVLRRILGTPTPPPLPDAGNLPADDKTFGGLTLREKLAQHKRDPRCSSCHL
                     RIDPMGFPLEGFDAVGRKRASYVDGTPVDVTGELKDGSTIVGADGLLTYLQSQGAKVM
                     TTLARKMLGYALGRNPQASDRPLLREMSEPSGEASFADLATQLVTSRQFRHRGVGVAA
                     PPAHQAQPKPAAAGVGAAPLAESP"
BASE COUNT          294 a          709 c          702 g          352 t
ORIGIN      
        1 gtggtggaat ccccgaagcg atccagccgg acgcccatgc ggtccatcag cgacaggtgc
       61 agactgcacg ccttccggtg ctcgtcgcct ttgtccaggt agtcgaggat gcggcccgtc
      121 gcaagcgatc ccccgccttt gccggtcagc aggacgggca gctggttggc gctgtgcagg
      181 tcgccgtcga acaggttcga cgccatcatc aggatcgagt tgtcgagcag cgtcgtctcg
      241 ccctccgcga tgtccttcat ccgctgtgtg aggtaggcga actgctcgat gtggaactga
      301 ttggtcttca ggtacatcgc ttccttgtcg gcagcgcgcc cgttgtgcgt gaggtcgagg
      361 tgcaaggcgc cctggacgcc gtcgaggaac ttgaagttca tctgcgacag gtcgttgttg
      421 agcatcagcg tcgccactcg cgtcttgtcc atctggaacg cgagcacgat caggtcgagc
      481 atcagcttca tgtgcgccgg cacgtcctgc ggcagctcgt gcttcgggcg cggcatgtcg
      541 ggctgcgcga gcgtgggccg ccagccttcg agccgctcct ccgtcgaggc ccgctcgatc
      601 cgcttctcga tgtcgcggat gccctcgaag tactcgctca gcttgtggtc gtcgtccttg
      661 ctgatgcgag gccgcaggcc ctgcgactcg tcccacaccg cgtcgaggat gctgcggtcg
      721 agcgtgcggc cggacccgtc gtccaccagg cggtcgaacg tccgggcggg atagatctcc
      781 ttggcggccg cccgcgtggg cgagatccac gagaggctcg acccgtagat catcgagagc
      841 ccgtcctcga gccggagctc gttgggctcg atgccgagca ccaggctcgg gacctcggtc
      901 tcgtccgcga aggccgtggc cagcacctgg tccatcgacg tgccgacgcg gatctggttg
      961 ggatcgaggc tgacgggcgc tcccgacagc aggttcatgc ggccgaggtg ggggctcgtc
     1021 gagacctgcg ccgactggtt gaagagcccc cggatgaaca ccatgtcttc ccgatgcggc
     1081 agcatcggct ccaggccagg cccgagctcc atgtcggcgc cgctgccctt cgcccaccag
     1141 tgcgccggct cgacgccgtt cgagaagtag atgatgccca ggcggaccgg gggcttcgtc
     1201 gcccctgcgc tgcccttgac cggctgcgcg aaagcaggca gcgactccat ccacggaaga
     1261 gtcagggcgg cgcccaggcc ccgcaagacg ttgcgccgcg acggcgtgga aacgcgacgc
     1321 ctcctgctca tggactctct gccaaaggtg ccgccccgac gcccgccgcg gcaggcttgg
     1381 gctgcgcctg gtgcgccggc ggcgcggcga cgccgactcc gcgatggcgg aactggcggc
     1441 tcgtcacgag ctgcgtggcc aggtccgcga acgacgcttc gcccgaaggc tcgctcatct
     1501 cgcggaggag cggccgatcg gacgcctgcg ggttccgtcc gagcgcgtag cccagcatct
     1561 tcctcgccag ggtcgtcatg accttggctc cctggctctg caagtatgtt agcagcccat
     1621 cggccccgac gatcgtcgat ccgtccttga gctcacccgt cacatcgacc ggcgtgccgt
     1681 cgacgtagct cgcgcgcttg cggcccaccg cgtcgaagcc ctcgagcggg aagcccatgg
     1741 ggtcgatgcg caagtggcac gaggagcacc gcggatcgcg cttgtgctgc gccagctttt
     1801 cgcgcagcgt cagcccgcca aaggtcttgt cgtccgccgg caggttgccc gcgtccggca
     1861 gcggcggcgg cgtcggcgtt ccgaggatgc gccggaggac ccagtccccg cgcttgacgg
     1921 gactcgtgcg cagcggcgcc gaggtcgtgg tgagcaccga tccgagccgc aacgccccac
     1981 cgcggtcgaa cgcgcgcgcg ccttcgacgc gttcgactcg atcgttggag ctgacgccgg
     2041 cgtcgatccc gtagaag
//
LOCUS       DATBSW010000007         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_776209, whole genome shotgun sequence.
ACCESSION   DATBSW010000007 DATBSW010000000
VERSION     DATBSW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776209"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VIF63_00255"
     CDS             complement(<1..429)
                     /locus_tag="VIF63_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY8237845.1"
                     /translation="MSHVLGIDVSTTATKAVLVDEAGAVAGIGVAEYGFDVPRPGWAE
                     QEPALWWDGTVAAIRSVLASTGVAGADIVAIGLTGQMHGLVLLDGADRVLRPAILWND
                     QRTAHACDEIREAVGPDRLIAITGNDAVTGLTAPKLVWVRD"
     gene            complement(464..1807)
                     /gene="xylA"
                     /locus_tag="VIF63_00260"
     CDS             complement(464..1807)
                     /gene="xylA"
                     /locus_tag="VIF63_00260"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY8237846.1"
                     /translation="MAGSFFAEIAGPIRYGGPDATDPLSFAAYDPDRVVLGKRMEDHL
                     RIGVCLWHSFAWPGSDMFGVGTFDRPWLAASLDPIEAATLKVDAAFEFLSKLGVPFFC
                     LHDRDVAPEGRDARDTRRIFDTVMALVEARMAETGRRLLWGTANLFTHPRFMSGAATN
                     PDPEVFAVAAAQVKLMLETTQRLGGQNYVLWGGREGYDTLLNTDLAREDRQLGRFLHL
                     VAEHKHRIGFEGALLIEPKPHEPAKHQYDRDAATVHGFLVRHGLEAEYRLNLEANHAT
                     LSGHSFHHEVAYAVAHGLLGSIDANRGDDQNGWDTDQFPNSVDELSLAVYEILKGGGF
                     TTGGFNFDAKLRRQSVARADLFHAHITGIDTLAQALLAAADMVERETLSKAVEARYAG
                     WDGELGRSILGGDLSLEDLARTVDDNGLDPKPRSGHQELLEGEVNRRIWRAGAGR"
     gene            2050..3234
                     /locus_tag="VIF63_00265"
     CDS             2050..3234
                     /locus_tag="VIF63_00265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8237847.1"
                     /translation="MTDPRYRAANRRRIRAAARQAMLVVVATSIVNLVWLVPAHPHEL
                     ARLVIANVVVSAAAIATASALRWRRRPPTEVMVFAVLIAIDLNSLLIAPIDLEVSFLV
                     AGYVLLMPPVVSLLIPWSARVHLAWVGVHAGIVGVISVVVPPEAHATGGPRVLLALML
                     VSSAVSVFGNLANLRARLIAFTQLEQIRVMNRKARRDDVRLRRLNALLTNLARTDELT
                     GLGNRVALQLQLQRIRGRIDRHGEQFAVLMLDLDHFKAINDRLGHSAGDAVLRQVGRA
                     IEAATRSSDAAFRYGGEEFVVVVEVHQPGDVAVVGERIRKAVADLGIGHPANAPYGTV
                     TISMGGCLVDRSRLATTDDAWFQSADAALYQAKRTGRNRSVIAGSGPAYAHRLTVAAL
                     PN"
     gene            complement(3265..>4395)
                     /locus_tag="VIF63_00270"
     CDS             complement(3265..>4395)
                     /locus_tag="VIF63_00270"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEY8237848.1"
                     /translation="YSPKFKALVPTFRFAAVGMVLITVLGSAQLFEAQLRLDTALTTS
                     PVVDFDPNAEPTDPGAEPSDAAGSTAGPAASTAPLPTDAPGPTAGITSCVGAAAIVRG
                     FDACPQDPAGKMRPEPVVAKTDIPAAYGDGCWMYAPFGAKRTCKYGSGEIQVALVGNS
                     HAGQWLPMLQVLAKKHGWTITTFLASQCNATDATLEMFTAAKNAGCLEYGRWVMEQTR
                     GARFDLVITSQRQSLRTVGDTWAETGVTAAAGYTTYLTRWSAAGTNLLVVQDTPFPHK
                     TVPSIPDCLARHRNDQAACAGTPDDWSRQDPLYDVATSLGLPTVSTLEVYRFLCAGNV
                     CPAVIGSVIVYFDGSHMTTTYARSIAPFVETEILAALARRAP"
BASE COUNT          634 a         1556 c         1521 g          684 t
ORIGIN      
        1 gtcgcgcacc cacaccagct tcggcgcggt cagccccgtg accgcgtcgt tgccggtgat
       61 ggcgatcagc cggtccggtc cgacggcctc tcggatctcg tcgcacgcat gggcggtgcg
      121 ctggtcgttc cacaggatcg cggggcgcag cacccggtcc gcgccgtcga gcaggacgag
      181 gccgtgcatc tggccggtga gcccgatcgc gacgatgtcc gcgcccgcga ccccggtcga
      241 cgccagcacg gaccggatgg cagccaccgt gccgtcccac cacagcgccg gctcctgctc
      301 ggcccagccg ggccgcggga cgtcgaaccc gtactccgcg acgccgatgc cggcaaccgc
      361 gccggcctca tcgacgagga cggccttggt cgccgtggtc gaaacgtcga tgccgagcac
      421 gtgactcatc gcgccgcctc ggttgcgcgc gccgccatga cggtcagcgg cctgccccgg
      481 cccgccagat ccgacggttg acctcgccct ccagcagctc ctggtggccg gatcggggct
      541 tcgggtccaa gccgttgtca tccaccgtcc gtgccaggtc ctcgagcgag agatcgcccc
      601 cgaggatcga ccgaccgagc tcgccgtccc agccggcgta gcgcgcctcg accgccttcg
      661 acagcgtctc gcgctcgacc atgtccgcgg cggcgagcag cgcctgggcg agcgtgtcga
      721 tgccggtgat gtgggcgtgg aacaggtcgg cccgggcgac gctctggcgc cggagcttgg
      781 cgtcgaagtt gaagccgccc gtcgtgaacc cgccgccctt gaggatctcg tacacggcga
      841 gcgacagctc gtcaacggag ttcgggaact ggtccgtgtc ccagccgttc tggtcatcgc
      901 cccggttggc atcgatgctg ccgagcagcc cgtgggccac ggcgtaggcg acctcgtggt
      961 ggaagctgtg gccggacagc gtcgcgtggt tggcctcgag gttcagcctg tactcggcct
     1021 cgaggccgtg ccggaccagg aacccgtgga ccgtcgcggc atcgcggtcg tactggtgct
     1081 tcgcgggctc gtgcggcttc ggctcgatca ggagcgcgcc ctcgaacccg atcctgtgct
     1141 tgtgctcggc gacgaggtgc aggaaccgcc ccagctggcg gtcctcgcgc gcgagatccg
     1201 tgttgagcag ggtgtcgtag ccctcgcgcc cgccccacag cacgtagttc tggccgccga
     1261 gccgctgcgt ggtctcgagc atcagcttga cctgcgccgc ggcgacggcg aagacctcgg
     1321 gatccggatt ggtcgccgcg ccggacatga accggggatg ggtgaacagg ttggcggtgc
     1381 cccagagcag gcgccggccg gtctcggcca tgcgcgcttc gacgagtgcc atcaccgtgt
     1441 cgaagatgcg gcgggtgtcc cgggcgtcgc gaccctccgg ggcgacgtcg cggtcgtgga
     1501 ggcagaagaa cggcacgccg agcttcgaga ggaactcgaa cgcggcgtcg accttcaacg
     1561 ttgccgcctc gatcggatcg aggctcgccg ccagccacgg ccggtcgaac gtgccgacac
     1621 caaacatgtc ggatcccggc cacgcgaacg agtgccacag gcaaacaccg atgcggaggt
     1681 ggtcttccat ccgcttgccg aggaccacgc ggtccgggtc gtacgcggcg aacgagagcg
     1741 ggtccgtcgc atcggggccg ccgtagcgaa tcgggccggc gatctccgcg aagaacgacc
     1801 cggccatttg cccctccctg ctgcgtcggc gtgccgatac tagatcggtc gagggtggcc
     1861 ggagccttta cggcagcgca ccgacatagg ccgcgtgggc acaacggccc gccccgaggt
     1921 gccaaccggc ccgtagcggt aacccggtcc cgccccgatc ctcgaacaat ggcccttacc
     1981 agcgagcgca agcccgcccc tcggagttgg cgaacctggt tcgaaggtgc gcgaggcgtc
     2041 ctcgcgggcg tgacggatcc ccgatatcgc gccgcgaacc ggcgccggat tcgtgccgcc
     2101 gcgcgtcagg cgatgctggt cgtcgtcgcg accagcatcg tgaatcttgt ctggctcgtg
     2161 cccgcccacc cccacgagct ggcgcgactg gtcatcgcca acgtcgtcgt ctccgcggcc
     2221 gcgatcgcta cggcatctgc cctccgctgg cgccgccgcc cgccgaccga ggtcatggtc
     2281 ttcgccgtcc tcattgcgat cgacctcaac tcgctgctca ttgcgcccat cgatcttgag
     2341 gtgtcgttcc tggtcgccgg gtacgtactg ctgatgccgc cggtcgtctc gctcctgatc
     2401 ccgtggagcg ctcgggtgca cctggcctgg gtcggcgtgc acgccggaat cgtcggcgtc
     2461 atctccgtgg tcgtgccacc cgaagcccat gcgaccggcg gtcccagggt cctgctcgcc
     2521 ctgatgctcg tctcctcggc cgtgagcgtc ttcggcaacc tggcgaacct gcgcgcgcgg
     2581 ctgatcgcgt tcacgcagct cgagcagatc cgggtgatga accgaaaggc gagacgggac
     2641 gacgtccggt tgcgccgcct caacgcgctg ctcacgaacc tcgcgcggac cgacgagctg
     2701 accggcctgg gcaaccgagt tgccctccag ctgcagctcc agcgaatccg aggtcggatc
     2761 gatcgccacg gcgagcagtt cgcggtgctg atgctcgatc tcgatcattt caaggcgatc
     2821 aacgaccgcc tcggccactc cgcaggcgac gccgtgcttc gccaggtggg ccgcgcgatc
     2881 gaggcagcga cccgctccag tgacgccgcg ttccgatacg gcggcgagga gttcgtcgtg
     2941 gtcgtcgagg tgcaccaacc tggcgacgtc gccgtggtcg gagaacgaat tcgaaaggcc
     3001 gtcgcggatc tcggcatcgg gcatccggcg aacgctccgt acggaaccgt gaccatctcc
     3061 atgggtggct gccttgtcga tcgcagccgg ctcgcgacga ccgatgacgc gtggttccag
     3121 agcgccgatg ccgcgctcta ccaggccaag cgcacgggac ggaatcggag cgtcatcgca
     3181 ggatccggac ccgcgtacgc gcaccggttg acggtcgcgg cgctgcccaa ctgagacgcg
     3241 gcgaccgcac gggcgtcgcc ggcgctacgg cgcgcgcctg gccaacgcgg cgaggatctc
     3301 cgtctcgacg aacggggcga tggatcgcgc ataggtcgtg gtcatgtgcg acccatcgaa
     3361 atagacgatg accgacccga tgaccgcggg gcacacattc cctgcgcaca ggaagcgata
     3421 gacctccagc gtcgagactg tgggcagccc gaggctcgtg gccacgtcgt agagcgggtc
     3481 ctggcggctc caatcatcgg gcgtcccggc gcaggcagcc tggtcgttcc gatgccgcgc
     3541 gaggcaatcc gggatcgagg gcacggtctt gtgcgggaac ggcgtgtcct gcacgacgag
     3601 gaggttcgtc cccgccgcgc tccagcgcgt caggtacgtg gtgtagccgg ccgcggccgt
     3661 gaccccggtc tcggcccacg tgtcgccgac ggtccgcagt gactggcgct gcgatgtgat
     3721 cacgaggtcg aacctggcgc ccctggtctg ctccatgacc cacctgccgt actcgaggca
     3781 gccggcgttc ttcgccgccg tgaacatctc gagcgtcgcg tcggtcgcgt tgcactgcga
     3841 cgcgaggaac gtggtgatcg tccagccgtg cttcttcgcc aggacctgga gcatcggcag
     3901 ccattgcccg gcgtgcgagt tgccgacgag cgcgacctgg atctcgccgc tgccgtactt
     3961 gcatgtccgc tttgccccga acggggcgta catccagcag ccatcgccgt acgccgccgg
     4021 gatgtcggtc ttcgcgacca ccggttcggg ccgcatcttc ccggccggat cctgggggca
     4081 ggcgtcgaag ccgcggacga tcgctgccgc gcccacgcac gacgtgatgc cggcggtcgg
     4141 accgggcgcg tcggtcggga gcggcgccgt cgaggctgcc ggtccggcgg tcgagcccgc
     4201 ggcgtccgag ggctcggcgc ccgggtcggt cggctcggcg ttcgggtcga agtcgaccac
     4261 cggcgacgtg gtcagcgccg tgtcgagccg gagctgcgcc tcgaacagct gggcactgcc
     4321 caggaccgtg atgagaacca tgcccaccgc cgcgaaccgg aacgtcggca cgagcgcctt
     4381 gaacttcggc gagta
//
LOCUS       DATBSW010000008         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1093847, whole genome shotgun sequence.
ACCESSION   DATBSW010000008 DATBSW010000000
VERSION     DATBSW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093847"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..142
                     /locus_tag="VIF63_00275"
     tRNA            61..142
                     /locus_tag="VIF63_00275"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:95..97,aa:Tyr,seq:gta)
     gene            147..222
                     /locus_tag="VIF63_00280"
     tRNA            147..222
                     /locus_tag="VIF63_00280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:180..182,aa:Gly,seq:tcc)
     gene            259..330
                     /locus_tag="VIF63_00285"
     tRNA            259..330
                     /locus_tag="VIF63_00285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:291..293,aa:Thr,seq:ggt)
     gene            360..1556
                     /gene="tuf"
                     /locus_tag="VIF63_00290"
     CDS             360..1556
                     /gene="tuf"
                     /locus_tag="VIF63_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY8237849.1"
                     /translation="MSKEKFVRNKPHVNIGTIGHVDHGKTTLTAAITKVQSTKGFAKF
                     TAFDQIDKAPEEKARGITISTAHVEYESDKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGAMPQTREHVLLGRQVNVPAILVYLNKCDMIGKGDEELLDLVEMELRELL
                     TKYNSPGETTPIIRGSALKALEGDTSEYGAPSIQKLMEACDSFIPEPKREIDKPYLMP
                     VEDVFTITGRGTVVTGRIERGIVKVGDELEIVGLGDTRLTTCTGVEMFRKLLDEGRAG
                     DNVGVLLRGTKREEVERGMVLCKKGTIAPHKKFVAEVYVLKKEEGGRHTPFFNGYRPQ
                     FYFRTTDVTGTVTLPEGTEMVMPGDNIKVDVELVSPIACEEGLRFAIREGGKTVGAGV
                     VTKIVE"
     gene            1574..1882
                     /gene="rpsJ"
                     /locus_tag="VIF63_00295"
     CDS             1574..1882
                     /gene="rpsJ"
                     /locus_tag="VIF63_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY8237850.1"
                     /translation="MTTPRIRIRLKAYDHKLLDQSAGEIVETAKRTGAKIAGPIPLPT
                     KINKYCVLRSPHTDKKSREQFEIRTHKRLLDILEPTQQTLDALMKLDLSAGVDVEIKT
                     "
     gene            1935..2657
                     /gene="rplD"
                     /locus_tag="VIF63_00300"
     CDS             1935..2657
                     /gene="rplD"
                     /locus_tag="VIF63_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8237851.1"
                     /translation="MPKLELKNTSGKAVGSIDLDDAVFGAEIHEHLLWETVKWQLAKR
                     RAGTASTKRLGEVRGSSKKVWKQKGTGQARQGSRQAPHWVGGATVFGPKPRDYGYQLP
                     RKAKKLALRSALSLRVSEQKLVILDGFATDGKTRSVATALAALGVAQPHHKVLIVDGK
                     DNANLVRGARNLPASQWIAPEGLNVYDILRHETLVLTQAAVKQLEAALSAPEHDEIPS
                     RSGDAASGGLSEGQIDSGVSHA"
     gene            2650..2979
                     /gene="rplW"
                     /locus_tag="VIF63_00305"
     CDS             2650..2979
                     /gene="rplW"
                     /locus_tag="VIF63_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8237852.1"
                     /translation="MREAQTIIKRPLLTEKTARLRETGGAAASPAEGDTYAQQVVFEV
                     SRDSNKIEIRRAVQDLFKVTVTDVRTVVIRGKQKRIGRFSGRRPTWKKAYVTLKAGDN
                     IEFFEGV"
     gene            2981..3805
                     /gene="rplB"
                     /locus_tag="VIF63_00310"
     CDS             2981..3805
                     /gene="rplB"
                     /locus_tag="VIF63_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8237853.1"
                     /translation="MAIKRYKPTSPGRRGMSSQDFGDVTKSEPEKSLLAHKSEKAGRN
                     NHGRITSRFRGGGHKQRYRVVDFKRKKTGVPATVIGIEYDPNRSARLALLQYADGEKA
                     YILAPQRLAVGDSVISANTADIKPGNSLPLRYIPIGTEVHAVELKVGRGAQIGRSAGT
                     KVTLMAKEGDWATLRMPSGEMRRVHIDCRATVGVIGNSEHANIEWGKAGRRRWMGIRP
                     HNRGVAMNPVDHPMGGGEGRSSGGRHPCSPWGQKTKGLKTRHNKRTDQFIIRRRSK"
BASE COUNT          765 a         1290 c         1273 g          509 t
ORIGIN      
        1 gagggggagg ctcaactggc tttgccagtt gagggggagc gggaaagctc ccccctataa
       61 ggagggattc ccgagtggcc aaagggagca gactgtaaat ctgccggcta tgccttcgaa
      121 ggttcgaatc cttctccatc cacccagcgg gtgtagctca gttggtagag ctccagcctt
      181 ccaagctggt tgtcgagggt tcgagtccct tcgcccgctc cagaaacctg atacaacgtc
      241 tccccgaaaa cagaatccgc cctcttagca cagtggcagt gcacctcctt ggtaaggagg
      301 gggtcccgag ttcaattctc ggagagggct cggcctaaaa aggaacaacc gagagcgtca
      361 tgtcgaaaga aaaattcgtc cgcaacaagc cccacgtcaa catcggcacg atcggccacg
      421 tcgatcacgg caagacgacg ttgaccgccg cgatcacgaa ggtccagtcg acgaagggct
      481 tcgcgaagtt cacggcgttc gatcagatcg acaaggcgcc ggaagagaag gcgcgcggca
      541 tcacgatctc gacggcgcac gtcgagtacg agagcgacaa gcgccactac gcgcacgtcg
      601 actgcccggg ccacgccgac tacatcaaga acatgatcac gggcgcggcg cagatggacg
      661 gcgcgatcct cgtggtctcc gcgccggacg gcgcgatgcc gcagacgcgc gagcacgtgc
      721 tgctcggccg ccaggtcaac gtgccggcga tcctcgtcta cctgaacaag tgcgacatga
      781 tcggcaaggg tgacgaggag ctgctcgacc tcgtcgagat ggagctccgc gagctcctca
      841 ccaagtacaa ctcccccggg gagaccacgc cgatcatccg cggctcggcg ctcaaggcgc
      901 tcgagggcga cacgtcggag tacggcgcgc cgtcgatcca gaagctgatg gaggcgtgcg
      961 actcgttcat ccccgagccg aagcgcgaga tcgacaagcc gtacctgatg ccggtcgagg
     1021 acgtgttcac gatcaccggc cgcggcaccg tcgtcacggg ccgcatcgag cgcggcatcg
     1081 tcaaggtcgg cgacgagctc gagatcgtcg gcctcggtga cacgcggctg accacctgca
     1141 ccggcgtcga gatgttccgc aagctgctcg acgagggccg ggccggcgac aacgtcggcg
     1201 tcctgctccg cggcaccaag cgcgaggagg tcgagcgcgg catggtgctc tgcaagaagg
     1261 gcacgatcgc gccgcacaag aagttcgtcg ccgaggtgta cgtgctgaag aaggaggagg
     1321 gcgggcgcca cacgccgttc ttcaacggct accggccgca gttctacttc cgcaccaccg
     1381 acgtgaccgg caccgtgacg ttgccggagg gcaccgagat ggtgatgccc ggcgacaaca
     1441 tcaaggtcga cgtcgagctc gtctcgccca tcgcctgcga agagggtctg cggttcgcga
     1501 tccgcgaggg cggcaagacc gtcggcgccg gcgtcgtgac caagatcgtc gagtaacgaa
     1561 ggtacagccc gccatgacga ccccacgcat ccgcatccgg ctgaaggcct acgaccacaa
     1621 gctcctcgac cagtcggccg gggagatcgt ggagaccgcc aagcgcacgg gcgccaagat
     1681 cgccggcccg atcccgctgc cgaccaagat caacaagtac tgcgtgctgc ggtcgccgca
     1741 taccgacaag aagtcgcgcg agcagttcga gatccgcacc cacaagcggc tgctcgacat
     1801 cctcgagccg acgcagcaga cgctcgacgc gctgatgaag ctcgacctct ccgccggcgt
     1861 ggacgtcgag atcaagacct gacccgggga tccccgggtc actgcgagtc cggatccaag
     1921 aaacgagaac tgtcatgccg aagctagagc tcaagaacac cagcggtaag gccgtcgggt
     1981 cgatcgacct cgacgacgcc gtgttcggcg cggagatcca cgagcacctc ctctgggaga
     2041 cggtgaagtg gcagctcgcg aagcgtcggg ccggcaccgc gtccaccaag cgcctcggcg
     2101 aggtccgggg cagctcgaag aaggtgtgga agcagaaggg caccggtcag gcccgccagg
     2161 gcagccgcca ggcgccgcac tgggtcggcg gcgccacggt cttcgggccc aagccccgcg
     2221 actacggcta ccagctcccc cgcaaggcga agaagctcgc gctgcggtcg gcgctgtcgc
     2281 tccgggtctc ggagcagaag ctggtcatcc tcgacggctt cgccaccgac ggcaagaccc
     2341 ggtcggtcgc gaccgcgctc gccgccctgg gcgtcgcgca gccgcaccac aaggtcctga
     2401 tcgtcgacgg caaggacaac gcgaacctcg tccgcggcgc gcgcaatctg ccggcgtcgc
     2461 agtggatcgc ccccgagggc ctcaacgtct acgacatcct tcgccacgag acgctggtcc
     2521 tcacccaggc ggcggtgaag cagctcgagg cggcgctgag cgcgccggag catgacgaga
     2581 tcccgagccg gtccggagac gcagcaagcg gaggactgag cgagggtcag atcgactctg
     2641 gagtaagcca tgcgtgaagc gcagaccatc atcaagcgcc cgctcctcac cgagaagacc
     2701 gcgcgactcc gcgagaccgg cggcgcggcc gcctcgcccg ccgagggcga cacctacgcc
     2761 cagcaggtcg tgttcgaggt ctcgcgggac tcgaacaaga tcgagatccg gcgcgcggtg
     2821 caggatctgt tcaaggtcac cgtcaccgac gtccgcaccg tggtcatccg cggcaagcag
     2881 aagcggatcg gccggttcag cggtcgtcgc ccgacctgga agaaggcgta cgtgacgctc
     2941 aaggccggcg acaacatcga gttcttcgag ggagtctgag atggcgatca agcggtacaa
     3001 gccgacctcc ccgggccgac gcggcatgtc gagccaggac ttcggcgacg tcacgaagag
     3061 cgagcccgag aagagcctgc tcgcgcacaa gtcggagaag gccggccgca acaaccacgg
     3121 ccgcatcacc tcgcgcttcc gcggcggcgg gcacaagcag cgctaccgcg ttgtcgactt
     3181 caagcgcaag aagaccggcg tgccggccac ggtgatcggg atcgagtacg acccgaaccg
     3241 ctcggcgcgg ctggcgctcc tgcagtacgc cgacggcgag aaggcgtaca tcctcgcccc
     3301 gcagcggctc gccgtcggcg actccgtgat ctcggcgaac accgccgaca tcaagcccgg
     3361 caactcgctg ccgctgcgct acatcccgat cggcaccgag gtgcacgcgg tcgagctcaa
     3421 ggtcggccgc ggcgcgcaga tcggccgctc ggccggcacg aaggtcacgc tgatggcgaa
     3481 ggagggcgac tgggccacgc tgcgcatgcc gtcgggcgag atgcggcggg tccacatcga
     3541 ctgccgcgcc acggtcggcg tgatcggcaa ctccgagcac gcgaacatcg agtggggcaa
     3601 ggccggccgc aggcgctgga tgggcatccg cccgcacaac cgcggcgtgg cgatgaaccc
     3661 ggtcgaccac ccgatgggcg gcggcgaggg tcgctcctcg ggcggccgcc acccgtgctc
     3721 gccgtggggc cagaagacca agggcctcaa gacccgccat aacaagcgca ccgaccagtt
     3781 catcatccgt cgacggagca agtagagatg cctcgttcag taaagaaggg tccgttc
//
LOCUS       DATBSW010000009         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1164371, whole genome shotgun sequence.
ACCESSION   DATBSW010000009 DATBSW010000000
VERSION     DATBSW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164371"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /gene="obgE"
                     /locus_tag="VIF63_00315"
     CDS             complement(<1..1138)
                     /gene="obgE"
                     /locus_tag="VIF63_00315"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY8237854.1"
                     /translation="MFLDRVKVFLRAGAGGDGASTFRREAHVPRGGPDGGDGGRGASV
                     YLQVDAGQTTLRDFQYKHHFRAEAGGGGQRARRHGKAGDDLILTVPPGTAVYDDATDE
                     LLADLVAMGQIAMVARGGRGGLGNVHFATSTHQAPRHAQKGEPGEERWVRLELRLIAD
                     IGLVGLPNAGKSTLLAGLTAATPKIADYPFTTLEPNLGVMDLAGEAPGLDADMRRPTI
                     ADVPGLIEGASAGAGLGHAFLRHVERTRVLVQVVDGSGRDPNWDFEVIREELRAHDPA
                     LLDKPTLIAFNKMDLPGAVEAWPAFAKARKRNGLPAVAIAASTGIGIPEFRAAIASLL
                     PSSAELDAPPDPTDVVVHRLDASPDRVTIRLESEGVYRVMGRRIE"
     gene            complement(1183..2415)
                     /locus_tag="VIF63_00320"
     CDS             complement(1183..2415)
                     /locus_tag="VIF63_00320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8237855.1"
                     /translation="MHRRAIGAARFLAHTLTPTARSRERAAAHTAAVAAPKPRASARR
                     RLYRRHLILRLTSDRTLPVAVALIVLVAAGVSLAPAAAPVGAAQGAAQANDTAVRLAV
                     GGAAGAGLAELDGQMQGGIGGEDVSDYVDDGTLYKPVAVDTSIQSSVGMLEHYKVKDG
                     DTLTGIASRFGVSMMTVWWANKITTKDSLKVGQDLIIPPVNGLVVTVKAGDTLDTLAA
                     TNKIDPEDIVNLNGLEDSVLIVGQVLVLPGAKGAPIPTPKPTPKPTKKPTYASSGGGA
                     GVAPKYNGGPWSWPVVGGGNYISQYFHYGHYGVDIAADYGSPVVAALAGTVVYAGWAN
                     TGCGYTVIIRHSANIYTMYCHNSAITVSRGEYVSRREQVARIGSSGWATGPHVHFAVS
                     IGYPHESGSYFVSALRYY"
     gene            complement(2806..>3651)
                     /gene="rho"
                     /locus_tag="VIF63_00325"
     CDS             complement(2806..>3651)
                     /gene="rho"
                     /locus_tag="VIF63_00325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8237856.1"
                     /translation="VHPDVLINLETDPKNLSQRLINLVNPLGKGQRALIVSPPKAGKT
                     MLLKSIANGISANYPDILLMVALIGERPEEVTDMRRSVKGEVISSTFDEPTENHTHVA
                     EMALEIAKRQVETGRDVVILLDSITRLARAYNLALPPSGRTLSGGLDPIALYPPKRFF
                     GAARNIEEGGSLTIIGTCLVDTGSRMDDVIYEEFKGTGNSELILDRKLAEKRIFPSVD
                     VMRSGTRREELLLEDGTLKLVWTMRRMLSAVGSNEGIELLLNRMAKTDTNAQFLLGLT
                     KSLDS"
BASE COUNT          523 a         1193 c         1352 g          583 t
ORIGIN      
        1 gttcgatgcg gcggcccatg acccgataga ccccttcgga ctcgagccgt atcgtcacgc
       61 gatccggcga agcgtcgagc cgatgcacga cgacgtcggt cgggtcgggg ggagcgtcga
      121 gctccgccga gctcggcagg agcgacgcga tcgccgcccg gaactccggg atcccgatcc
      181 ccgtagatgc cgcaatcgca accgcgggca ggccgtttcg cttccgtgcc ttcgcgaacg
      241 cgggccacgc ctccaccgca ccgggcaggt ccatcttgtt gaacgcgatc agggtcggct
      301 tgtcgaggag cgccggatcg tgggcgcgga gctcctcgcg gatgacctcg aagtcccagt
      361 tgggatcgcg gcccgagcca tcgacgacct gcacgagcac gcgcgttcgc tcgacatggc
      421 gcaggaaggc gtgcccgagt cctgcgccgg cgctggcgcc ctcgatcaag ccgggcacgt
      481 ccgcgatcgt cggccggcgc atgtcggcgt ccaggccggg cgcctcgccc gcgagatcca
      541 tcacaccgag gttcggctcg agcgtggtga aggggtagtc ggcgatcttg ggcgtggccg
      601 cggtgaggcc cgcgagcagc gtcgacttcc ccgcgttcgg cagcccgacc aggccgatgt
      661 cggcgatcag ccggagctcg aggcggaccc agcgctcctc gccgggctcg cccttctggg
      721 cgtgccgcgg ggcctggtgg gtggaggtcg caaagtgcac attgccgagg ccgcctcgac
      781 cgccgcgggc gaccatggcg atctggccca tggcgacgag atcggcaagc agctcgtcgg
      841 tcgcgtcgtc gtagacggcc gttccgggtg gcaccgtgag gatgaggtcg tcgccggcct
      901 tgccgtggcg ccgcgcccgc tggccaccgc cgcccgcctc cgcgcggaag tggtgcttgt
      961 actggaagtc gcgcagcgtg gtctgcccgg cgtcgacctg gaggtacacc gaggcgccgc
     1021 ggccgccgtc gccgccgtcc gggccgccgc ggggaacatg cgcctcgcgc cggaaggtcg
     1081 aggctccatc gccgcccgcg ccggcgcgca ggaagacctt cacacggtcg agaaacatcg
     1141 aatcaatctt cgcacgggcg gccgaggccg ccgcggaacg atctagtagt agcgcagcgc
     1201 gctgacgaag tagctgccgc tctcgtgggg gtagccgatc gaaaccgcga agtggacgtg
     1261 cgggccggtc gcccagccgc tgctgccgat ccgtgcgacc tgctcgcgcc gcgagacgta
     1321 ctcgccgcgc gacacggtga tcgccgagtt gtggcagtac atcgtgtaga tgttcgcgct
     1381 gtgccggatg atcaccgtgt agccgcagcc ggtgttcgcc caaccggcgt agacgacggt
     1441 cccggcgagg gcggcgacca cgggtgagcc gtagtcggcc gcgatgtcga cgccgtagtg
     1501 gccgtagtgg aagtactggc tgatgtagtt gccgccgccg accacgggcc acgaccacgg
     1561 accgccgttg tacttcgggg cgacgccggc gccaccgccg ctgctggcgt aggtcggctt
     1621 cttcgtgggc ttgggcgtcg gcttcggtgt cgggatcggc gcgcccttcg cgccggggag
     1681 gacgagcacc tggccgacga tgaggaccga gtcctcgagg ccgttgaggt tgacgatgtc
     1741 ctcagggtcg atcttgttcg tcgccgcgag ggtgtcgagc gtgtcaccgg ccttgaccgt
     1801 gacgacgagc ccgttgaccg gcgggatgat caggtcctgg ccgaccttga gcgagtcctt
     1861 cgtcgtgatc ttgttggccc accagacggt catcatcgag acgccgaagc ggctcgcgat
     1921 gccggtcagg gtgtcgccgt ccttgacctt gtagtgctcg agcatcccga ccgacgactg
     1981 gatgctcgta tcgaccgcga ccggcttgta cagcgtgccg tcgtcgacat agtcggacac
     2041 gtcttcgccg ccgatgccgc cctgcatctg cccatcgagc tcggcgaggc cagcaccggc
     2101 ggcgccgccg acggcgaggc ggaccgcggt gtcgttggcc tgggctgcgc cctgcgcggc
     2161 accgaccgga gccgccgcgg gcgcgaggct cacgccggct gccacgagga cgatcagggc
     2221 gaccgcgacg ggaagggtgc gatcggaggt gagccgcagg atcaggtgcc gacggtagag
     2281 gcggcgccgg gccgaagctc gcggcttggg ggctgcgacg gcagcggtgt gggctgccgc
     2341 gcgctcacgc gagcgtgcgg tcggggtcag ggtgtgcgcc aggaaccgcg cagcgccgat
     2401 cgcgcgccga tgcacgtgcc gcgtggtcgc tggtcgggcc ggctcgatca atggatcggg
     2461 cccattcgag cggaacagtg cgacagcgcg cactacgcca ctcctggcgt tcagcaatac
     2521 gttctcctgg cgagacggtc cggtgcgtgc gaaagcgcgg gcttcgcggg atggaccgtc
     2581 gacgggtcgc gatcttgagc tggggtctcg cttcgcggcc gagctcactc ctccgtgagc
     2641 tgcgacgttg cgcggacctt accacgcgcc tcgcgggccc tgcaaacggc cgacaggccc
     2701 ggccgagcac gcccgatggt cagggtggag gcctatgtca acacgcacca gcgacgcgcc
     2761 ggcgagccac gtttgagcgc gcccgtgagc gcgttgggca gcgcgctagg agtcgaggga
     2821 cttggtgagg ccaagcagga actgggcgtt cgtgtccgtc ttcgccatgc ggttgaggag
     2881 cagctcgatg ccctcgttcg aaccgacggc ggagagcatc cggcgcatcg tccagacgag
     2941 cttgagcgtg ccgtcctcga ggaggagctc ctcgcgccgg gtgccggagc gcatcacgtc
     3001 gaccgagggg aagatccgct tctcggcgag cttccggtcg aggatgagct ccgagttgcc
     3061 ggtgcccttg aactcctcgt agatgacgtc gtccatgcgc gatccggtgt cgacgaggca
     3121 ggtgccgatg atcgtcagcg aaccgccctc ctcgatgttc cgggcggcac cgaagaagcg
     3181 cttgggcgga tacagggcga tcggatcgag gccacccgac agcgtccggc cggacggtgg
     3241 cagggcgagg ttgtaggcgc gggccagacg ggtgatcgag tcgagcagga tgacgacgtc
     3301 acgcccggtc tcgacctggc gcttggcgat ctcgagcgcc atctcggcga cgtgggtgtg
     3361 gttctcggtc ggctcatcga aggtcgagct gatgacctcg cccttgacgc tccggcgcat
     3421 gtccgtgacc tcctcgggcc gctcgccgat gagggcgacc atgagcagga tgtcggggta
     3481 gttggcgctg atgccattgg cgatgctctt gaggagcatc gtcttgccgg ccttgggcgg
     3541 gctgacgatg agcgcgcgct ggcccttgcc gagcggattg acgaggttga tgagccgctg
     3601 gctgaggttc ttcgggtccg tctcgaggtt gatcaggacg tccgggtgga c
//
LOCUS       DATBSW010000010         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_141701, whole genome shotgun sequence.
ACCESSION   DATBSW010000010 DATBSW010000000
VERSION     DATBSW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141701"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..674
                     /locus_tag="VIF63_00330"
     CDS             222..674
                     /locus_tag="VIF63_00330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8237857.1"
                     /translation="MEYRVRAARITDIEQLVALSGARLGDASSGSLGATDILRQLVFL
                     PHATVLVAESKRTIVGGAVLAIRPSVRAGGYVGTIDLLVVDPTSEPDAVSDSLIEECL
                     KSAANKGCASVEAPQPDDPAEHPRWERQGFKDAAPVLRRDVDPAGRRR"
     gene            724..2607
                     /locus_tag="VIF63_00335"
     CDS             724..2607
                     /locus_tag="VIF63_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY8237858.1"
                     /translation="MGKNVAVFVDVANIFYAAKAAGVDIDYVTLLKAGSAGRDLVRAY
                     AYTGLDPDNENQRNFHDFLRRSGYKVVSKDIRKYGDGKVKANLDIELVVDMMKTARNL
                     DVAIVVSGDGDFAPAIRAVQEMGVRVEVVSFRGNTSSDLMDVADLFTDIIQIAKVERG
                     SSRSGRRVAGDDDLSMTEVPDKQSEGTGERTRGGRGRTRRTTATPVTAATAPPSRSRA
                     RVAAVESGEAPLIVALPGEKLSRAAAVAAPSAAPDDLDIDAEDMDDELEPTETSLDGP
                     VGEESAEETRRRRRRRGGRGRGRSRGREEGAFVDAPAGASDEEVIVDPDDIPGEADEM
                     PAPARRAASAFGSVWDSQIGTPAGRRQTPALDDEEDFPEPAIPEYLITERRQRDGGRG
                     GGAGRGRGGAYAAALDRERFGGGRGSRPSSFNQPAPAPREGGFNRPDRGGRNDRVDRI
                     DRGDRGGQRPPFRPAPAPAPRGSGDEPWSEVPPEIEAMLRAQLGSRPAPQRAAPAADP
                     VMESASPAPARRGRRPAAAEAEAPMVADDGGPATAEAPAKARRTPARRTRAAAAKAEA
                     GTTDTADATPTAKPAAKRASAAAKPRTTRAKAAAPAAETSDAGADVAPKRRAPRKKAD
                     PSA"
     gene            2651..3880
                     /locus_tag="VIF63_00340"
     CDS             2651..3880
                     /locus_tag="VIF63_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8237859.1"
                     /translation="MATDMFAAGRFRTRGQRAALDAVIAMLATGMPHAVLLVGPPGVG
                     KGTLAEDIAAGLLCRAPDGPRPCRECRGCRALEHGNHPDVHRLEPVGAGSVIPIGGRE
                     ERGVRDLVRDLALLPVEGGARVAIVSAADRMTEDAQSAFLKTLEEPPAATVLILTAAD
                     EERLLETIRSRVVRIRLGPVGRPDVEQILVDADAADAPLAARLARLSAGRPGDAMALA
                     RVPEALTIRAEIGRTLLDLAVATRADRLRLGRDLLARAGELAAAIRRGTGASEPASST
                     QRRPARGRKGAVERPAPAAAPAAQVSGDADTEAPAPEAAGPASRVPPAERRAAALTLV
                     AIWRDVARDLAVVAFGEPGSVRQLDLLDDLEASTARLPASFAAAQLRRLEVAGERLEG
                     NVSPELVVDALALGWAA"
     gene            3877..4173
                     /locus_tag="VIF63_00345"
     CDS             3877..4173
                     /locus_tag="VIF63_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY8237860.1"
                     /translation="MSDEARLDAVVHGRVHGVGFRVFVLRIARATGLRGWVANEPGRV
                     HVVAEGAQAELERLLEAIRTGPPAALVERVDAAWPPATGELQGFEVRSGWHGGD"
     gene            4287..>4514
                     /locus_tag="VIF63_00350"
     CDS             4287..>4514
                     /locus_tag="VIF63_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237861.1"
                     /translation="MDSVPVAEVLPGLYRAVLDAVADLESHNHRAEAAVIRFEATRVY
                     SRAWTPDAARRLEALRIRAGRIVNTSPRTRYD"
BASE COUNT          646 a         1664 c         1649 g          555 t
ORIGIN      
        1 ggcggctccg aacggcgcgc aggtggccgc cgaagcgatg ccgtatgata gggcccggcg
       61 caccgattgc aaggccggtc gttcgcgacc cgggacgatt tcagacgcac cacgtgcgtg
      121 actcccgtga ggtccgcgag cccggcggtc tggtcggcca cggtcgcgac gacccgcaca
      181 tcgaaatcag cgcctccgac agctagggag acccccggcg cgtggagtac agggtccgag
      241 cggcccggat aacggacatc gagcaactcg tcgccttgag cggcgcgcgt ctcggtgacg
      301 cctcgtccgg ctcgctcggc gccacggaca tcctgcgcca gctcgtgttc ctgccccacg
      361 caaccgttct cgtcgcagag agcaaacgga ccatcgtggg gggcgccgtg ctcgcgatcc
      421 ggccttcggt tcgcgccggc gggtatgtcg gaaccattga tctgctcgtc gtcgacccga
      481 cgagcgaacc ggacgccgtg agcgacagcc tcatcgagga atgcctcaaa tcggcggcca
      541 acaagggatg tgcgtcggtc gaggcgccgc agccggacga cccggcagag cacccgcgct
      601 gggagcgcca ggggttcaag gacgcagcac cggtcctccg gcgcgacgtc gaccccgcag
      661 gacgccggcg ctgaatcggg cgcgcgccac tcggcgcatg cgcccaacga gataggagac
      721 agggtgggca agaacgtcgc cgtcttcgtg gacgtggcga acatcttcta cgcggccaag
      781 gcggccggag tggacatcga ttacgtcacg ctgctgaagg cggggagcgc ggggcgagac
      841 ctcgtgcgcg cctacgcata tacggggctc gacccggaca acgagaacca gcgcaacttc
      901 cacgacttcc tgcgccggag cggctataag gtcgtgagca aggacatccg caagtacggc
      961 gacggcaagg tcaaggcgaa cctcgacatc gagctcgtcg tcgacatgat gaagaccgcc
     1021 cggaacctcg acgtcgcaat cgtcgtgtcg ggcgacggcg acttcgcccc ggccatccgc
     1081 gccgtccagg agatgggcgt ccgcgtcgag gtcgtgagct tccgcggcaa cacgtcctcg
     1141 gacctgatgg acgtcgccga cctgttcacg gacatcatcc agatcgccaa ggtcgaacgc
     1201 ggctcgtccc gctccggccg ccgggttgcc ggtgacgacg acctgtcgat gaccgaggtg
     1261 ccggacaagc agtccgaggg caccggcgag cgcacgcgcg gcggccgagg ccgtacccgt
     1321 cgcacgactg ccacgccggt gacggcagcg acggctccgc caagccgaag ccgcgcgcgc
     1381 gtcgccgcag tcgaatccgg ggaggcgccg ctcatcgtgg cgctcccggg cgagaagctg
     1441 tcccgtgccg cggcggtcgc cgccccgagc gcggcgccgg acgacctgga catcgacgcc
     1501 gaagacatgg acgacgagct cgaaccaaca gagacgagcc tggacggccc ggtcggcgag
     1561 gagtcggccg aggaaacccg tcgccgacgt cgccgtcgag gtgggcgagg ccgcggccgc
     1621 agccgagggc gcgaggaggg cgcgttcgtc gatgcccctg ccggcgccag cgatgaggag
     1681 gtcatcgtcg atcccgacga cattccgggc gaagccgacg agatgccggc tccggcacgc
     1741 cgggccgcct ccgcgttcgg ttcggtgtgg gactcccaga tcggcacccc ggctggtcgt
     1801 cgccagacgc ctgccctcga tgacgaggag gacttccccg agccggccat tcccgagtac
     1861 ctgatcacgg agcgccgtca gcgcgacggc ggccgtggtg gtggcgcggg acggggccga
     1921 ggcggcgcct atgccgccgc tctggaccgc gagcggttcg gtggcggccg cggcagccgg
     1981 ccctcgagct tcaaccaacc ggcgcccgcg ccgcgtgagg gtggcttcaa ccgcccagat
     2041 cgcggtggtc gcaacgatcg agtcgatcga atcgatcgcg gcgatcgcgg tgggcagcgg
     2101 ccgccgttcc ggcccgcacc ggcgcccgcg ccgcgcggct cgggcgacga gccctggagc
     2161 gaggtcccgc cggagatcga ggcgatgctg cgagcgcagc tcggctcgcg cccggcaccg
     2221 cagcgagccg ctccggcagc tgacccggtg atggagtcgg cctcgccggc gcccgctcgc
     2281 cgtggccgac gcccggcagc tgccgaggcc gaggctccga tggtcgccga tgatggcggc
     2341 ccggccacgg ccgaggctcc ggcgaaggcg cgacggacgc ccgctcggcg gacccgcgct
     2401 gccgcggcaa aggccgaggc aggcacgacg gacacggcgg atgcgacccc gacggccaag
     2461 ccggcggcga agcgagcctc cgcggccgcg aagccgcgga cgacccgggc gaaggcggcc
     2521 gcgccagccg cggagacgtc cgacgccggc gccgacgtgg cgccgaagcg ccgggcgccg
     2581 cgcaagaagg ccgatccgtc ggcgtagccc gctccgggag aacgcacttg gcctgcgcgc
     2641 gcggatcgtg atggcgacgg acatgttcgc cgccggtcgg ttccgcacgc gcgggcagcg
     2701 cgcggcgctc gatgccgtca tcgcgatgct cgcgacgggc atgccccacg ccgtgctgct
     2761 cgtcggtccg ccgggcgtcg gcaaaggcac gcttgccgag gacatcgccg cgggcctgct
     2821 gtgtcgggcg cccgacggcc cccggccctg tcgcgaatgc cgcggctgtc gcgcgctcga
     2881 gcacggcaac catcccgacg tccaccggct cgagccggtc ggggcggggt cggtcatccc
     2941 gatcggcggc cgtgaggagc gcggcgtgcg cgacctcgtt cgcgacctcg cactcctgcc
     3001 cgtggagggc ggcgcgcgcg tcgcgatcgt gagcgcggcc gatcgcatga ccgaggacgc
     3061 ccagtcggcg ttcctcaaga ccctcgaaga accgcccgcg gcgacggtgc tgatcctcac
     3121 ggcagcggac gaggagcgcc tcctcgagac gatccgttcg cgcgtcgtcc ggatccgcct
     3181 cgggccggtc gggcgaccgg acgtcgagca gatcctcgtc gacgccgatg ccgccgacgc
     3241 gccactcgct gcccgactcg cgcgcctctc cgccggccgg cccggcgacg cgatggccct
     3301 cgcccgcgtg ccggaggccc tcaccatccg ggccgagatc gggcgcaccc tgctcgacct
     3361 tgcagtcgcc acacgcgcgg accgcctgcg actcgggaga gacctgcttg cccgcgcggg
     3421 cgagctcgcg gccgccatcc ggcgcggcac aggcgcgagc gagccggcga gttcgaccca
     3481 acgccgtccc gcgcgcggtc gcaagggtgc ggtggagcgc ccggcacctg cagcggcacc
     3541 ggccgcccag gtctctggcg acgccgacac cgaggcgcct gcccccgagg cggccggccc
     3601 ggcatcccgg gtgccccccg ctgagcgtcg cgccgccgcc ctcaccctcg tcgccatctg
     3661 gcgcgacgtc gcgcgcgatc tcgcggtcgt ggccttcggc gagccgggca gcgtccgcca
     3721 gctcgacctg ctcgacgatc tcgaagcgtc gacggcccgg ctgccggcgt cgtttgccgc
     3781 cgctcagctg cggcggctcg aggtcgcggg cgagcgcctc gagggcaacg tgagcccgga
     3841 gctcgtcgtc gatgcgctcg cgctcggctg ggctgcgtga gcgacgaagc gcggctcgac
     3901 gccgtcgtcc acgggcgcgt ccacggggtc ggctttcggg tgttcgtgct ccgaatcgca
     3961 cgtgcgaccg gcctgcgcgg ctgggtggcg aacgagcccg gccgggtcca tgtggtcgcc
     4021 gagggcgccc aggcagagct ggagcggctc ctggaggcga tcaggacggg cccgccggct
     4081 gcccttgtcg agcgcgtcga tgccgcctgg ccgccggcta cgggcgagct ccaggggttc
     4141 gaagttcggt ccggctggca cggcggcgac tgaccgagaa gacccaccgc tcgcgcgaac
     4201 gtgcgcgaac gtgaacgaac gtgcggtgcc ggtggtacca gcgactcatg gtgcgagcgc
     4261 ctgcctggcc gcatccttcc gctcccatgg attcagtgcc cgtcgccgag gtcctccccg
     4321 ggctgtatcg cgccgtgctc gatgcggtcg ccgatctcga gtcgcacaac catcgcgccg
     4381 aggccgccgt gatccgcttc gaggcgacgc gcgtctactc gcgcgcctgg acgcccgacg
     4441 cggcacgccg gctggaagcc cttcgcatcc gcgccggacg catcgtcaac accagcccgc
     4501 gaacccggta cgac
//
LOCUS       DATBSW010000011         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_776574, whole genome shotgun sequence.
ACCESSION   DATBSW010000011 DATBSW010000000
VERSION     DATBSW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776574"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..>3021
                     /gene="secA"
                     /locus_tag="VIF63_00355"
     CDS             41..>3021
                     /gene="secA"
                     /locus_tag="VIF63_00355"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8237862.1"
                     /translation="MLNFFSRFVDSNDRELKRIQPFVDEANDLEAEIQALTDAEIRER
                     FTAIRDEFRATVLPEEPTDDELHHPDLERRRELGKARRKRDIAAIQDALDDVLPDVFA
                     MAREAMQRTLGMRHFDEQLIGGVVLHQGKIAEMRTGEGKTLVAPLAAILNAIADRGVH
                     VVTVNDYLARRDPQWMGPIFHFLGLTVGMILGHQSDGGAASYVFEPGFPTNDERLINL
                     RPVTRREAYAADVTYGTNNEFGFDYLRDNMVTDLESRVQRERYFGIVDEVDNILIDEA
                     RTPLIISGQAEESGDLYYTFAKLVPKLRERRADWVEGDPEDGDYFIDLKDKAVSPTEG
                     GVDRIEKLLGVDNIYDADPRYARHFEQALRAHALYKRDRDYIVKEGEIIIVDEFTGRQ
                     MPGRRWSEGLHQAIEAKEGLRVQRESVTMATITFQNYFRLYDKLAGMTGTAMTEAEEF
                     HKIYQLEVVAVPTHRPMVRADDTDLVYLNEKAKFNAVIDEIEAMQEAGRPVLVGTVSV
                     EKSEKLGALLQQRGIKHEVLNAKFHERESGIVAQAGRTGAVTIATNMAGRGTDILLGG
                     NPQGLASDILHRKGLNVAEVDKETYAAALAEAKLVTEEDHERVVNAGGLHIVGTERHE
                     SRRIDNQLRGRAARQGDPGSSRFYLSLDDDLMKRFASERVASLMERMGLDEDNYIESG
                     IVGKTIESAQTRVEGFNFDMRKRVVEFDDVINKQRETIYAERDKVLRNEDLTETIRAF
                     LDEDLDEVVERHLGGPNEAVTEEGLTDLLVDLGRMGLGADRIDHDDLLRLSSAGAISE
                     HVRDVADALLEAKEAEVGAEAWATIERFVLLRTIDSLWVEHLTEIDDMRRGIGLRGYA
                     QQDPLNEFRKEAFRLYEELSGFIRHQVASTIFRVSIQQPQQQQAGGVFAMQGPGQRGP
                     GAAGAVGAARPGRAGARQGVMAGPGVSGTSGSAGMAGVAGVAGAGVAGAGGAGGQQAR
                     PGYTPSGAKIGRNDACWCGSGR"
BASE COUNT          527 a         1052 c         1045 g          397 t
ORIGIN      
        1 gaccgggtat ggacgagctc gcacctatac ttcgaggccc atgttgaatt tcttttcccg
       61 gttcgtcgac tcgaacgacc gtgagctgaa gcggatccag ccgttcgtcg acgaggccaa
      121 cgatctcgaa gcagagatcc aggccctgac cgacgcggag atccgcgagc gcttcacggc
      181 cattcgcgac gagttccgcg cgaccgtcct gcccgaggag ccgaccgacg acgagctcca
      241 ccaccccgac ctcgagcgcc ggcgcgagct cggcaaggcg cgccgcaagc gcgacatcgc
      301 ggcgatccag gacgccctcg acgacgtcct gccggacgtg ttcgcgatgg cccgcgaggc
      361 gatgcagcgc acgctcggga tgcgccactt cgacgagcag ctcatcggcg gcgtcgtcct
      421 ccaccagggc aagatcgccg agatgcgcac cggcgagggc aagacccttg tcgcgccgct
      481 cgccgcgatc ctcaacgcga tcgccgaccg gggcgtccac gtcgtcacgg tcaacgacta
      541 cctcgctcgc cgagacccgc agtggatggg cccgatcttc cacttcctcg ggctgacggt
      601 cgggatgatc ctgggccacc agtccgatgg cggggccgcg tcgtacgtgt tcgagcccgg
      661 cttcccgacg aacgacgagc ggctgatcaa cctccgcccg gtcacccggc gcgaggcgta
      721 cgccgccgac gtcacgtacg gcacgaacaa tgagttcggc ttcgactacc tccgcgacaa
      781 catggtcacc gacctcgagt cgcgggtgca gcgcgagcgc tacttcggca tcgtcgacga
      841 ggtcgacaac atcctcatcg acgaggcccg cacgccgctg atcatcagcg gccaggctga
      901 ggagtcgggc gacctctact acacgttcgc gaagctcgtc ccgaagctgc gcgagcggcg
      961 cgccgattgg gtcgagggcg atcccgagga cggcgactac ttcatcgacc tcaaggacaa
     1021 ggcggtctcg ccgaccgagg gcggggtcga tcggatcgag aagctgctcg gcgtcgacaa
     1081 catctacgac gccgatccgc gctacgcccg ccacttcgag caggccctcc gcgcccacgc
     1141 gctgtacaag cgcgatcgcg actacatcgt caaagagggc gagatcatca tcgtcgacga
     1201 gttcaccggg cggcagatgc cgggccggcg ctggagcgag ggcctgcacc aggcgatcga
     1261 ggccaaggag gggctgcggg tccagcgcga gagcgtgacg atggccacga tcacgttcca
     1321 gaactacttc cggctctacg acaagctcgc cggcatgacc ggcacggcga tgaccgaggc
     1381 cgaggagttc cacaagatct accagctcga ggtcgtcgcg gtgccgaccc accggccgat
     1441 ggtccgcgcc gacgacaccg acctcgtcta cctcaacgag aaggcgaagt tcaacgcggt
     1501 catcgacgag atcgaggcga tgcaggaggc cggccggccg gttctcgtcg gcaccgtgtc
     1561 ggtcgagaag tcggagaagc tcggcgcgct gctccagcag cgtgggatca agcacgaggt
     1621 cctgaacgcg aagttccacg agcgcgagtc gggcatcgtc gcccaggcgg gccgcaccgg
     1681 cgcggtgacc atcgccacga acatggccgg ccgcggcacg gacatcctgc tcggcggcaa
     1741 cccacagggc ctcgcctcgg acatcctcca ccgcaagggc ctcaacgtgg ccgaggtgga
     1801 caaggagacg tacgccgccg cgctggccga ggcgaagctg gtcaccgagg aggaccacga
     1861 gcgcgtggtc aatgccggcg gcctgcacat cgtcggcacg gagcggcacg agagccggcg
     1921 catcgacaac cagctccgcg gccgcgccgc ccgccagggc gacccgggct cgtcgcgctt
     1981 ctacctctcg ctcgacgacg acctcatgaa gcgctttgcc tcggagcgcg tcgcgagcct
     2041 catggagcgg atgggcctcg acgaggacaa ctacatcgag tcgggcatcg tcggcaagac
     2101 gatcgagagc gcccagacgc gcgtcgaggg cttcaacttc gacatgcgca agcgggtcgt
     2161 cgagttcgac gacgtcatca acaagcagcg cgagacgatc tacgccgagc gcgacaaggt
     2221 cctgcgcaac gaagacctca ccgagacgat ccgggcgttc ctcgatgagg acctcgacga
     2281 ggtcgtcgag cgccatctcg gcgggccgaa cgaggcggtc accgaggagg gcctcaccga
     2341 cctgctcgtc gacctgggcc ggatgggcct cggcgccgat cgcatcgacc acgacgacct
     2401 gctgcggctc tcgtccgctg gcgcgatctc ggagcacgtc cgcgacgtcg ccgacgccct
     2461 gctcgaggcc aaggaggccg aggtcggcgc ggaggcgtgg gccacgatcg agcgattcgt
     2521 gctgctgcgc acgatcgact cgctgtgggt cgagcacctg accgagatcg atgacatgcg
     2581 ccggggcatc ggcctgcgcg gctacgccca gcaggatccg ctcaacgagt tccgaaagga
     2641 ggcgttccgc ctctacgagg agctctcggg cttcatccgc caccaggtcg cgagcacgat
     2701 cttccgggtc agcatccagc agccccagca gcagcaggcc ggcggcgtgt tcgcgatgca
     2761 gggccccggc caacgcgggc cgggagcggc gggcgcggtc ggcgccgcgc gaccgggtcg
     2821 ggccggcgcc cgccagggcg tcatggccgg gccgggcgtg tccggcacgt cgggctcggc
     2881 gggcatggcc ggggtcgccg gggtcgccgg cgcgggcgtg gcgggtgcgg gcggcgcggg
     2941 cgggcagcag gcacgaccgg gctacacgcc gagcggcgcc aagatcgggc gcaacgacgc
     3001 ctgctggtgc ggctccgggc g
//
LOCUS       DATBSW010000012         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_600332, whole genome shotgun sequence.
ACCESSION   DATBSW010000012 DATBSW010000000
VERSION     DATBSW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600332"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VIF63_00360"
     CDS             complement(<1..849)
                     /locus_tag="VIF63_00360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY8237863.1"
                     /translation="MTRSAVVEGGFYGPGSEAWRLNREAMLLLGAGPRALLLQVAHPL
                     VAAGVAEHSDFRADPWARLSGTLRSYLRIVYGTAGAARDEIGRLHELHRGVRGDVVEP
                     AARSRHGGRYSALDPDLALWVHATLVDSTIAAYDAWIEPLPRADRARYYAETLPVGRA
                     FGIPADRLPPDLDAFEAYLAEMLAPGGPLAIGEVARELASAILNPPLGPAVAAAGEPF
                     GRLAPLLDGIPSRAYSWLFWPAIGLLPPRIREGYELPWGLRQRVVAGWLVSTWQAWRP
                     MLPPSFR"
     gene            complement(854..1501)
                     /locus_tag="VIF63_00365"
     CDS             complement(854..1501)
                     /locus_tag="VIF63_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEY8237864.1"
                     /translation="MTIGMPRMDRLPAQERGLTIGDFLVPIRVSERLHTRARHLALIL
                     IGTALIAVSARVYLVIPGNPVPFTGQTFGVLLSAGALGFRRGVAATVLYLLIGMLGLP
                     VFAEGKHGIEVVLGASGGYLIGFVVASAIVGRLAELGWDRNVFGAVGAMLIGSLVIYA
                     IGVPWLAATAFDGDLAVAFDKGMRTFLLWDALKLALAAVAFPAAWWFVGRRPGDR"
BASE COUNT          256 a          537 c          594 g          170 t
ORIGIN      
        1 ccggaacgag ggcgggagca tcggtcgcca ggcctgccag gtggacacga gccagcccgc
       61 gacgacgcgc tggcgcaggc cccacgggag ctcgtagccc tcgcggatgc gcggcggcag
      121 caacccgatt gccggccaga agagccacga gtaggcgcgg ctcgggatgc cgtcgagcag
      181 cggcgcgagg cgcccgaacg gctcgccggc ggcggcgacc gccggcccga ggggcggatt
      241 caggatcgca cttgcgagct cccgagccac ctccccgatc gcgagcgggc cgccgggcgc
      301 gagcatctcg gcgagatagg cctcgaaggc gtcgaggtcc ggcggcagcc gatcggcggg
      361 gatcccgaag gcacggccca cgggcaacgt ctcggcgtag tagcgcgccc ggtccgcgcg
      421 cggcaagggt tcgatccacg cgtcgtacgc cgcgatcgtc gagtcgacga gggtcgcgtg
      481 gacccacaac gcaaggtcgg gatcgagcgc gctgtaccga ccgccgtggc gcgagcgggc
      541 cgcaggctcg acgacgtcgc cgcggacgcc gcgatggagc tcgtggagcc ggccgatctc
      601 atcgcgtgcc gcgccggccg tcccgtagac gatgcgcagg tacgagcgca gcgtgcccga
      661 gaggcgcgcc cacgggtcgg ctcggaagtc cgagtgctcg gcgacgccgg cggcgacgag
      721 cgggtgcgcg acctggagca ggagtgcccg cggcccggcg ccgaggagga gcatcgcctc
      781 gcgattgagc cgccacgctt cgctgccggg gccgtagaag ccgccctcga caacggcgct
      841 tcgggtcacg tgcctagcgg tctcccggcc gacggccgac gaaccaccac gcggcgggga
      901 acgcgacggc ggccagcgcg agcttcagcg cgtcccacag caggaacgtg cgcatgccct
      961 tgtcgaatgc gaccgcgagg tcgccgtcga atgccgtggc agcgagccag ggcacgccga
     1021 tggcgtagat cacgaggctg ccgatgagca tggcgccgac ggcgccgaac acgtttcgat
     1081 cccagcccag ctcggcgagc ctgccgacga ttgcggaagc gaccacgaaa ccgatgaggt
     1141 agccaccgga ggcgccgagc acgacctcga tcccgtgctt gccctcggcg aacacgggca
     1201 agccgagcat gccgatgagc aggtacagca cggtcgccgc gacgcctcga cggaagccga
     1261 gtgcgccggc cgagagcagg acgccgaacg tctggccggt gaacggcacg ggatttccgg
     1321 ggatcacgag gtacaccctt gcgctcacgg cgatgagcgc ggtgccgatc aggatcaggg
     1381 cgaggtgccg ggcgcgcgtg tggaggcgct cggagacgcg gatcgggacg aggaagtcac
     1441 cgatcgtcag gccgcgctcc tgggcgggca ggcgatccat gcgcggcatg ccgatcgtca
     1501 ttcgcggatg tcctcctcgc cctcggcgac ccgaggcgca agcgccgatc gatgggg
//
LOCUS       DATBSW010000013         7798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_776712, whole genome shotgun sequence.
ACCESSION   DATBSW010000013 DATBSW010000000
VERSION     DATBSW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776712"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VIF63_00370"
     CDS             <1..363
                     /locus_tag="VIF63_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8237865.1"
                     /translation="LVAFSAVTAATQLTVGDIRGSEAASAMLRAVMAEAWTVGRALGV
                     PLADDLVDRQHALVLAQRDDEAASLRHDLITGHRMELEALQGTLRRLGRETSIPTPWT
                     DAAYAILQPWAIRNERNA"
     gene            complement(365..772)
                     /locus_tag="VIF63_00375"
     CDS             complement(365..772)
                     /locus_tag="VIF63_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237866.1"
                     /translation="MQNYVVRLVAASLVVVSVAACSTGAAPTATPALTAVPAFAIRAA
                     AAQPQACMDALLGGKLTRNPGSGLGVTNGNEATVVEWPFRYSAREAEGRVLLIDETGK
                     VVAREGDEITVGGGFGNQFWHACGPVTVTKAAN"
     gene            914..1138
                     /locus_tag="VIF63_00380"
     CDS             914..1138
                     /locus_tag="VIF63_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY8237867.1"
                     /translation="MKVSVSLPEADVEFLDRYTRQAGLESRSAALQKAVRLLRTAELS
                     DEYAAAWEEFDTDGAAALWETTVGDGIDAR"
     gene            1135..1485
                     /locus_tag="VIF63_00385"
     CDS             1135..1485
                     /locus_tag="VIF63_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY8237868.1"
                     /translation="MRRGDIHIVDFEPARGSEVDKRRPAIIVSNDASNAIVTRLGRGL
                     LTVVPLTTNIARLYKFQVLLPAARTGLDSDSKAQAEQVRSVGVERVGDRIGLVPYSLL
                     DRVDEALRIHLDLG"
     gene            1505..3040
                     /locus_tag="VIF63_00390"
     CDS             1505..3040
                     /locus_tag="VIF63_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8237869.1"
                     /translation="MTETLTPASAGSAGTAPTFRLFIAGAWVESVSGQTFESLNPADR
                     RDVVGRFQAGTKADVAMAIRAAEMALPRWKATPAPKRGEILYRFGELMAQHKERLSRA
                     MTREMGKVLAEARGDVQEGIDIAYLMAGEGRRMFGDTVPSELPDKWAMSIRQPIGIAG
                     IITPWNFPVAIPCWKSMPALVAGNTIVFKPASDTPHCATLVVELMEQAGFPAGVVNLV
                     TGSGAEVGDAIVDSPDVPVISFTGHSSTGKSIAERGARRLKRVSLELGGKNAVVVMRD
                     ADLDLATDGILWSAFGTTGQRCTACSRVIVEKDVVEPLLEKLESRAKKLRLGSGLDET
                     VDVGPLINPAALEKVSGYIDIGRSEGELVMGGAPATDGELAHGNFFSPTIFIGVMPMA
                     RIAQEEIFGPVLSVIPVDDYQGAVTALNQTRYGLSSAIFTRDANTAFRAMRDFETGIV
                     YVNAGTTGAETHLPFGGWKETGNGHREAGHAALDSFTEWKSIYVDFSGRLQRAQIDNQ
                     EQV"
     gene            3137..3505
                     /locus_tag="VIF63_00395"
     CDS             3137..3505
                     /locus_tag="VIF63_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237870.1"
                     /translation="MAPTGLAALFIGSGTLHLLRPKLFAAAVPAALPAPGAIIATSGI
                     AELICAGALLSGHRWAGPISALLLVAIFPGNLTVALATETDPGASRRARVAAWARLPL
                     QVPLIWAALQAGKATKRPQT"
     gene            3623..4117
                     /locus_tag="VIF63_00400"
     CDS             3623..4117
                     /locus_tag="VIF63_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237871.1"
                     /translation="MKNRLVAPILLTLLLAACSGGPSVTTKPGATSGPAATAKPGGTV
                     DCEAMKTAAQQLLGVQLLAQLKTPDTIESIKTKAFGNLDLDTFISALQVLHALDGYPS
                     VLGDPKAAIDFYIKAAEAAKVLFATEPMTQAAIDTYNQNVGTVGDFLAKQSAISGAMG
                     EAGC"
     gene            4156..6516
                     /locus_tag="VIF63_00405"
     CDS             4156..6516
                     /locus_tag="VIF63_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8237872.1"
                     /translation="MKAPLTVRIATWSARHRWLVFVLWFVATLGTLGAGLAAGGINTI
                     DVDEDPNGPRLESEVAYEVLGAGEPVAPAERLVVVIDGGPGAALDPAFQAQVHLIAAE
                     LTAAKATVDGAETSVFESVIDPFVVPPEAAAGVISPDGSTVQVVGNIPGEAPIVEQKL
                     VPVPAIIEAARARLPNARVHLISSTILNRDFSELISSELDGALYLTIPLTFVILLLAF
                     GAIAAALIPLVLAATSLAAGYGLLGLYSQSVGPVSANAAQLLILMGLAVAVDYSLFEV
                     TRFRSERQRGASIHEAIETASSTAGRAVFFSGIAVIISLGGLLTLGVSLFTSMAVGTM
                     GVVAVSVIGSLTFLPATMSIVGDRVNFGRPATWLPRLARLTGPEPIRVRGQFALDALE
                     RRAARPPGSGFWATLVNAVMGRPVILTVLSAAVLLTIAAPALRLRTGTTDVTALPSTI
                     DGIAGIKLINEKFPFGQDIRMDVVVTQPTRADVAAAIETLLTRVDALEGMGEGRLVRT
                     ADDGHAALISFQMTGNRNDEANRALVRQVRTEVVPDVFGAASLSDVDVYVAGQAARTL
                     DIVGIYEGATPRIFGFVLGLSFLLMLVAFRSIVIPIKAILLNLLSTAAAFGVLVLVFQ
                     EGWFADALGITPGGVIESWVPIFIFTILFGLSMDYHLFILTRIKEARDRGLDSRAAVA
                     RGISVTSGTITSAASIMVVVFAVFVTFKFVFIQQLGLGLSVAVFIDATLIRSVLLPAT
                     MTLLGDWNWWLPRWLGWLPHITIEGANGEPEELEPAGAPAKAPAQA"
     gene            6520..6858
                     /locus_tag="VIF63_00410"
     CDS             6520..6858
                     /locus_tag="VIF63_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY8237873.1"
                     /translation="MPTFDDVRSIALALPEVEEHVTWGADINFRVRNKMFVIGGEGAD
                     HVTIKASISEQADLLDLEGETFTSAAYVGRYGWVTVDLGRVDPLMLETLVRNAWRSTA
                     PAKLRGLLPE"
     gene            6855..>7798
                     /locus_tag="VIF63_00415"
     CDS             6855..>7798
                     /locus_tag="VIF63_00415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8237874.1"
                     /translation="MNAVRGDQEAAFEVRRATPADEPALLQLGHESAIHHAAIDPDRW
                     RVPSLEEEAEARRYWHAARPRDEAFVAVADGQVVGVVELWLVRPRKPAGARIARTKAD
                     LGISVAPEWRGHGIGTALLREAEVWARSRGAERMMLDLDASNTGALRLYERMGYWVHG
                     LEMDKPIDPDPASVDDPAIIRNVDGDPVPTLAGESVVLRPLRDGDRDALLAVLQDPTV
                     VAVWDSRGAENSVNELFAGDKNWTVWAIEVDGEFAGSIQAAENDDDDYKSAGIDIFMS
                     SRFQGRGLGTDAVRTLARYLIEVRGHHRLTIDPAADNAR"
BASE COUNT         1197 a         2689 c         2685 g         1227 t
ORIGIN      
        1 ctcgtggcgt tctcggcggt caccgcggcg acccagctga ccgtcggcga catccgcggc
       61 tccgaggcgg cgtccgcgat gctacgggcg gtgatggcgg aggcgtggac ggttggccgc
      121 gccctcggcg tcccgctcgc cgacgatctc gtcgatcggc agcacgccct cgtcctcgcg
      181 caacgggacg atgaggcggc gtcgctgcga cacgacctga tcaccgggca ccggatggag
      241 ctcgaggcgc tccagggaac cctgcgccgg ctgggacgcg agacgagtat cccgacgcca
      301 tggaccgacg cggcctacgc gatcctccag ccctgggcga tccgcaacga gcgcaacgcc
      361 tgatctagtt cgccgccttc gtcaccgtga ccgggccgca ggcgtgccag aactggttgc
      421 caaagccgcc cccgacagtg atctcgtcac cctcgcgagc cacgaccttg cccgtttcgt
      481 cgatgagcag gacgcggcct tcggcctcgc gagcgctgta acggaacggc cattcgacga
      541 ccgtcgcctc gttgccattc gtgactccga ggcccgaccc gggattgcgc gtgagcttcc
      601 cgccgagcaa ggcgtccatg cacgcctggg gctgcgccgc ggcagcgcga atggcgaagg
      661 cgggcacggc cgtcagggcc ggcgtggccg tcggcgccgc gccagtcgaa caggccgcaa
      721 cggaaacaac gacgagcgaa gcggcaacga gccgcacgac gtagttctgc atgacgtcct
      781 tccaatgacc gttgtccgac cagcgtctcg tggcctcatc gggccggttc gtctcgccca
      841 gacggtcgca gtggttcccg cggttcccgc tcaacccatg ctacccagtt gctacccatg
      901 gtacgatcag ggtatgaagg tcagcgtgag cctgccggag gcggacgtcg agttcctcga
      961 ccggtacacg aggcaagccg ggctcgagtc gaggtccgcg gcgctgcaga aggcggtccg
     1021 gctgcttcgg acggcggagc tgagcgacga gtatgcggcc gcttgggagg agttcgatac
     1081 cgatggtgcg gccgccctgt gggagaccac cgtcggcgac ggcatcgatg ctcgatgaga
     1141 cgcggagaca tccacatcgt cgacttcgag cccgcccgcg gctccgaggt cgacaagcga
     1201 cggccagcga tcatcgtgag caatgacgcc tcgaacgcca tcgtgacacg cctcgggcgg
     1261 ggccttctga ccgtcgtccc gctcacgaca aatatcgcac ggctctacaa gtttcaggtg
     1321 ctcctgccgg cggcgcgcac gggcctcgac agtgactcga aggcccaggc cgaacaggtt
     1381 cgctcggtcg gggtcgaacg cgtcggcgat cggatcggcc tcgtgcccta ctcgctgctc
     1441 gaccgcgttg acgaggcact caggatccac ctcgacttgg gctgatgggc actatcctgc
     1501 ccgcatgaca gagacgctga ctccggcgtc ggcaggatcg gccggcacgg cgccaacctt
     1561 caggctgttc atcgccggtg cgtgggttga atccgtttcg ggccagacgt tcgagagcct
     1621 gaacccggcc gatcgacgcg acgtcgtcgg gcgattccag gcaggcacga aggcggacgt
     1681 cgcgatggcc atccgcgccg ccgagatggc cctgccgcgc tggaaggcga ccccggcacc
     1741 gaagcgcggc gagatcctct atcgcttcgg tgagctgatg gcccagcaca aggagcgcct
     1801 gtcgagggcg atgacccgcg agatgggcaa ggtgctcgcc gaggctcgcg gcgacgtcca
     1861 ggaagggatc gacatcgcct acctcatggc cggcgagggc cggcggatgt tcggcgacac
     1921 ggtgccatca gagctgccgg acaagtgggc gatgagcatc cgccagccga tcggcatcgc
     1981 cgggatcatc acgccctgga acttcccggt cgcgattccg tgctggaagt cgatgccggc
     2041 gctcgtcgcc ggcaacacca tcgtgttcaa gccggcgagc gacacgccgc actgcgcgac
     2101 gctcgtcgtc gagctcatgg agcaggccgg cttcccggcg ggcgtcgtga acctcgtgac
     2161 cgggtcgggc gcggaggtcg gcgacgccat cgtcgactcg ccggatgtgc cggtgatctc
     2221 gttcacgggc cattcatcga ccggaaaatc gatcgcggag cgcggcgcgc gccgtctcaa
     2281 gcgtgtctcg ctcgagctgg gcggcaagaa cgcggtcgtg gtcatgcgcg acgccgacct
     2341 cgacctcgcg accgacggca tcctctggtc ggcattcggc acgaccggac agcgctgcac
     2401 cgcgtgctcg cgggtgatcg tcgagaaaga cgtcgtcgaa ccgttgctcg agaagctcga
     2461 gtcgcgggcc aagaagctcc gcctgggctc gggcctcgac gagacggtcg atgtcgggcc
     2521 gctcatcaac ccggccgcgc tcgagaaggt cagcggctac atcgacatcg ggcggtccga
     2581 aggcgagctc gtgatgggcg gcgccccggc gaccgacggc gaactcgccc acggcaactt
     2641 cttctcgccg acgatcttca tcggggtcat gccgatggcg cgaatcgcgc aggaggagat
     2701 cttcgggccg gtgctgtcgg tcatccccgt cgatgactac cagggcgccg tcaccgcgct
     2761 caaccagacg cgctacggcc tgtcctcggc gatcttcacc cgtgacgcga acacggcgtt
     2821 ccgcgcgatg cgcgacttcg agaccggcat cgtctacgtc aacgccggca cgaccggcgc
     2881 cgagacgcat ctgcccttcg gcggctggaa ggagaccggc aacggccacc gcgaggccgg
     2941 ccacgccgcc cttgactcgt tcaccgagtg gaagtcgatc tacgtcgact tctcgggccg
     3001 cctccagcgc gcccagatcg acaatcaaga gcaggtatga gcgtcacgtg tcgtcaccct
     3061 gactgagcgg gtgtgcgagt ttgttgaggc gcaagcagcc catgaatcag cccgtcgttt
     3121 gagtcggatc cgccgcatgg cgcccacggg gctcgccgca ctcttcatcg gcagcggcac
     3181 gcttcacctg ctgcgaccga agcttttcgc ggccgcggtg ccggcagcgc tgccggcacc
     3241 cggcgcgatc atcgcgacga gcgggatcgc cgagctcatc tgcgccggcg ctctgctttc
     3301 gggacatcgg tgggcaggtc ccatcagtgc gctcctgctg gttgccatct ttcccggcaa
     3361 tttgacggtc gccctcgcga cggagacgga tccgggcgcc tcacgtcgcg ctcgcgtcgc
     3421 agcgtgggct cgactaccgc tgcaggttcc cctgatctgg gccgcccttc aggccggtaa
     3481 ggccaccaag cggccccaga cgtaggcccc ggggcgagtc tgcagccgcg ttgcgcgcgg
     3541 gccagggcca actgaatacc tagcctccgg ccgaactatt ggcggacggc ggcggattga
     3601 gcggaggggc taggcttcga ggatgaagaa cagactcgtc gcaccgatcc tgctgaccct
     3661 cctcctcgcc gcctgcagcg gcggaccttc cgtgacaacg aaaccgggag ccaccagtgg
     3721 tcccgcggcg accgcgaagc cgggaggtac ggtcgactgc gaggcgatga agaccgccgc
     3781 gcagcagctg ctcggcgtgc agctcctggc gcagctgaag acgccggaca cgatcgagtc
     3841 gatcaagacc aaggcgttcg gcaacctcga tctcgatacg ttcatcagcg cgctgcaggt
     3901 gttgcatgcg ctcgatggct acccgtcggt gctcggcgat cccaaggcgg cgatcgactt
     3961 ctacatcaag gccgccgagg cagcgaaggt cctctttgcc accgagccga tgacgcaggc
     4021 ggcgatcgac acctacaacc agaacgtcgg cacggtgggc gacttcctcg ccaagcagtc
     4081 ggcgatctcc ggcgcgatgg gcgaggccgg ctgctgaagc gccgcggcgg agtccctcgc
     4141 tagactctgc cgttcatgaa ggccccgctg acggtccgta tcgccacgtg gtcggcgcgc
     4201 caccgctggc tcgtgttcgt cctttggttc gtcgcgacgc tcggcacgct gggggcgggg
     4261 ctcgctgccg gcgggatcaa cacgatcgac gtcgacgagg acccgaacgg cccgcgcctg
     4321 gagtccgagg tcgcctacga ggtgctcggt gccggcgagc cagtggctcc ggcggagcgg
     4381 ctcgtcgtcg tcatcgacgg cggtcccggc gctgcgctcg acccggcgtt ccaggcacag
     4441 gtccacctca tcgcggcgga gctgaccgcc gcgaaggcga ccgtcgacgg cgcggagacg
     4501 tccgtcttcg agagcgtcat cgacccgttc gtcgtgccgc cggaagccgc cgccggcgtc
     4561 atctcgcccg acggctcgac cgtccaggtg gtcggcaaca ttcccggtga ggcgccgatc
     4621 gtcgagcaaa agctcgtgcc ggtgccggcg atcatcgagg cggcgcgcgc ccgcctcccg
     4681 aatgcccgtg tccacctcat cagcagcacc atcctcaacc gcgacttcag cgagctcatc
     4741 tcgagtgagc tcgacggggc gctgtacctg acgatcccgc tgacgttcgt gatcctgctg
     4801 ctcgcgttcg gcgcgatcgc cgcggcgctg atcccgctcg tcctcgccgc gacctcgctg
     4861 gccgccggct acgggctcct gggcctgtac agccagtcgg tcggaccggt cagcgcgaac
     4921 gcggcgcagc tcctgatcct gatgggcctc gcggtcgccg tggactactc gctgttcgag
     4981 gtcactaggt tccgctccga gcggcaacgc ggcgcgagca tccacgaggc gatcgagacg
     5041 gcgagcagca ccgccggccg tgcggtgttc ttctcgggca tcgcggtgat catctcgctc
     5101 ggcggtctcc tgaccctcgg cgtctcgctg ttcacgtcga tggccgtggg cacgatgggc
     5161 gtggttgccg tgtcggtcat cggcagcctc accttcctgc ccgcgacgat gtcgatcgtc
     5221 ggcgatcggg tgaatttcgg ccggccggcg acgtggctgc cgcgcctcgc ccgactgacc
     5281 ggtccggagc cgatccgggt gcgcgggcag tttgcgctcg acgccctgga gcggcgagcg
     5341 gcgcggccgc cgggcagcgg cttctgggcg acgctcgtga atgccgtgat gggccggccg
     5401 gtgatcctga ccgtcctctc ggcggccgtg ctcctcacga tcgctgcccc ggcgctgcga
     5461 ctccgaaccg gcacgaccga cgtcacggcc ctgccctcga cgatcgacgg catcgccggc
     5521 atcaagctca tcaacgagaa gttcccgttc ggacaggaca tccggatgga cgtcgtcgtg
     5581 actcagccaa cccgcgccga cgtagcggcg gcgatcgaga cgctgctgac gcgcgtcgac
     5641 gcgctcgagg gaatgggcga ggggcggctc gtgcgcacgg ccgacgacgg ccacgccgcg
     5701 ctgatctcgt tccagatgac cggcaatcgc aacgacgagg cgaaccgtgc cctcgtgcgc
     5761 caggtccgca ccgaggtcgt gcccgacgtg ttcggcgcgg cgagcctgtc tgacgtcgac
     5821 gtctacgtgg ccggccaggc cgcgcgaacg ctcgacatcg tcggcatcta cgagggcgcg
     5881 acgccgcgca tcttcggctt tgtgctcggg ctgtcgttcc tcctgatgct cgtggcgttc
     5941 cgctcgatcg tcatccccat caaggcgatc ctgctgaacc tgctctcaac cgccgccgcg
     6001 ttcggcgtgc tcgtgctggt gttccaggag ggctggttcg ccgacgcgct cgggatcacg
     6061 ccgggcggcg tcatcgagag ctgggtcccg atcttcatct tcacgatcct gttcgggctg
     6121 tcgatggact accacctgtt catcctcacc cggatcaagg aggcgcgcga tcgcggcctt
     6181 gactcgcggg ccgcggtggc gcggggcatc tcggtcacat ccggcacgat cacgagcgcc
     6241 gcctcgatca tggtcgtcgt ctttgccgta ttcgtgacct tcaagttcgt gttcatccag
     6301 cagcttggac tcgggttgtc ggtggcggtg ttcatcgacg cgacgctgat tcggagcgtg
     6361 ctcctgccgg cgacgatgac cctgctcggc gactggaact ggtggctgcc gcgctggctc
     6421 ggctggctgc cgcacatcac gatcgagggc gcgaacggcg agcccgagga actcgagccg
     6481 gccggtgcgc ccgccaaggc gccggcacag gcctgaccga tgccgacctt cgacgacgtt
     6541 cgatcgatcg ctctggccct gcccgaggtc gaggagcacg tgacgtgggg cgcggatatc
     6601 aacttccggg tccgcaacaa gatgttcgtc atcggcggcg agggcgccga ccacgtgacg
     6661 atcaaggcat cgatctcgga gcaggccgac ctgctcgacc tcgaggggga aacgttcacg
     6721 agcgcggcgt acgtcggtcg gtacggctgg gtcacagtgg accttgggcg cgtggacccg
     6781 ctgatgctcg agacgctcgt gcgcaacgcc tggcgctcga ccgcgcccgc gaagctgcgc
     6841 ggcctgctgc cggaatgaac gcggtgcgtg gcgaccagga ggctgcgttc gaggtccggc
     6901 gcgcgacgcc cgcggacgag ccggcgctgc tccagctcgg gcacgagagc gcgatccatc
     6961 acgccgccat cgacccggat cgatggcgcg ttccatcgct ggaggaggag gccgaggcac
     7021 gccggtactg gcacgcggcc aggccgcgcg acgaggcgtt cgtcgccgtt gccgacgggc
     7081 aagtcgtggg cgtcgtcgag ctgtggctgg tccggccgcg caagccggcc ggcgcacgga
     7141 tcgcgcgaac gaaggctgac ctcgggatca gcgtggcacc cgagtggcgc ggccacggca
     7201 tcggcacggc gctgctgcgc gaagcggagg tctgggcacg gtcgcgcggc gccgaacgga
     7261 tgatgctcga cctcgacgcg agcaataccg gcgcgcttcg cctctacgag cgaatgggct
     7321 actgggtcca cggcctcgag atggacaagc cgatcgaccc ggacccggcg agcgtcgatg
     7381 acccggccat catccggaac gtcgatggcg acccggtccc gacgcttgcg ggcgagtcgg
     7441 tcgtgctgcg accgctccgc gacggcgatc gcgacgcgct cctcgccgtg ctgcaggacc
     7501 cgacggtcgt ggcggtctgg gactcgcgcg gcgccgagaa cagcgtcaac gagctgttcg
     7561 ccggcgacaa gaactggacc gtctgggcga tcgaggtcga cggcgagttc gcgggcagca
     7621 tccaggccgc cgagaacgac gatgacgact acaagagcgc gggcatcgac atcttcatgt
     7681 cgtcgcgatt tcagggtcgc ggtctcggca ccgatgcggt gcggacgctc gcccgctacc
     7741 tcatcgaggt gcgcggccat caccggctga cgatcgatcc cgcggcggac aacgcccg
//
LOCUS       DATBSW010000014         6540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_353451, whole genome shotgun sequence.
ACCESSION   DATBSW010000014 DATBSW010000000
VERSION     DATBSW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353451"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VIF63_00420"
     CDS             <1..425
                     /locus_tag="VIF63_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237875.1"
                     /translation="YFWLLGRVDDVMNVSGHRLSTTEIESALVSHPAVAEAAVVGGPD
                     AVTGEAINCFVILRKGVEATDELALELRNHVSTKISPIAKPKSLMFTPDLPKTRSGKI
                     MRRLLRDIAQGRALGDTTTLADASVVETIRENSGKSEE"
     gene            705..1373
                     /locus_tag="VIF63_00425"
     CDS             705..1373
                     /locus_tag="VIF63_00425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8237876.1"
                     /translation="MQSVQAVVFDVGETLVDETRAWSAWADHLGVPRLTFMTALGASL
                     ARGESHREVFELFAPGREIDAEYRRLVAARDFPVVAPEDLYPDALPCLEALAADGYRL
                     GIAANQPAETADALREASIEVELMAMSDAWGLYKPDPAFFARIAEELALPPAAIAYVG
                     DRLDNDVGPAHAAGMIAIFVRRGPWAWINTPRGEPPEADLVVESLAELPIRLRRSPFG
                     RRGS"
     gene            complement(1333..2607)
                     /locus_tag="VIF63_00430"
     CDS             complement(1333..2607)
                     /locus_tag="VIF63_00430"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8237877.1"
                     /translation="MPQANATTESTRPTVVVALSEPERSEVVEVLNEAGFDTIPMATS
                     APLVEAFSPSTPTLIALVDIAGDPEGAVARVQEARKGRPGALSVIYSATEAELDGLEA
                     AGLDIDEIILRPWSADAIRWRVEAVAIRLQAPTSTSTDAVLAGGHLDGAWGATGAPVF
                     AVFNPKGGVGKTTIATNLAAVLQIRKGMQVLLLDADTVTGHVGLSLGLPAGRSAADSW
                     IDEDAGYAHETLLDLATTHSSGIKVVALSTNPLSHSNLPPGRVADAIMEARQGVDAVV
                     VDLHPSYSEVNLAIFAIADRILVPVTPDLPAMRAAIQLKEVALEVGVRDRLAMIINRA
                     NSGVTVADMEATVGLNAIAHIRSAGLHFVWSANAGKSLIDKFPRHPAAQDFEHLADRL
                     ISVHAGEPMPERRRDTASLLKNLFGRKVISEA"
     gene            complement(2607..3965)
                     /locus_tag="VIF63_00435"
     CDS             complement(2607..3965)
                     /locus_tag="VIF63_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237878.1"
                     /translation="MTSNQKKGFRLPWGTARPDEGGTNLLDPAVLESRIDVKRDDLGD
                     GPSRPSRGARSKTTDAAPAAADVPVETPEAAMNDADESLTESAAAPPDENGDVNGVWP
                     TFDRRATDRRAADRRGTDRPFMNPQAVAEQRLPRRDNPLVAGLVKAMREAAIASRGET
                     TARLEAEATARVEAIRARATTEVAELRKRSEEDITSIRDWSKIEIARVRQETDDKIDQ
                     RKVELAGETERHAATVDQLVEQVQTTVAAFEADMERFFERLLAETDPATLATLAEQAP
                     DPPDLRGEGPAATDFIGDASMDDEAMADASMDAPMDAPVNAPDGLAAGAAAEAEAEAT
                     EGLDPLGIEDWPTAVLTAAHRSDAVQTPAEPNGPDSTRLFVSGLTSVAGISAFKGALG
                     QLDGVRSVSVSSGEAGVFVFAVSHDPGVDLDSALSHLTGFAAQVTDATDDGFTVVAAE
                     AA"
     gene            4135..5217
                     /gene="moaA"
                     /locus_tag="VIF63_00440"
     CDS             4135..5217
                     /gene="moaA"
                     /locus_tag="VIF63_00440"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY8237879.1"
                     /translation="MLDRTATRKASATGTASAPVAAQPRDRLGRALHDLRISVTDRCN
                     FRCPYCMPSEVFGRDYQFLPRAEILSYEEIHRLATVFVGLGVRKLRLTGGEPTLRRGL
                     PDLVAMLASIEAPDQQPLDIAVTTNGSALRQLARPLADAGLRRVTVSLDSLDDETFRR
                     MNGVDFPVAKVLDGIDAAVEAGLGPVKINVVLKRGENDAGILDLARWARDAGHVLRFI
                     EYMDVGTTNGWRLDEVVPAAEVIETIGRAWPVEPEAPTYPGEVAERYRYLDGKGEIGV
                     IASVTRPFCGDCTRARLSAEGKLYTCLFSAVGHDLRDPLRSGASDADLAAGVAGIWAG
                     RDDRYSERRAAATDRLPKIEMFALGG"
     gene            5606..6451
                     /locus_tag="VIF63_00445"
     CDS             5606..6451
                     /locus_tag="VIF63_00445"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY8237880.1"
                     /translation="MDPATPIPPIDQPARLDALEAAVRARTDLVPELGIVLGSGLGGL
                     ADELRDAVAIPFADLPGWPAATAPGHVGRLLLGTLAGKPVVMLQGRFHLYEGNAPGLV
                     VQPVLLFKQLGAATVILTNAAGGLDPSFGPGTLMVIADHINLTGQNPLIGPNAPSIGD
                     RFPDVTDAWSPRLRSKLHAAGAAEGVKLAEGIYVGLTGPNYETPAEVRMYAAFGGHAV
                     GMSTVMECIAARWAGLEVCGISLVTNAGAGYSGKPLTHEEVLAAGAEAGPRFSRVIRR
                     FIADL"
BASE COUNT          919 a         2275 c         2319 g         1027 t
ORIGIN      
        1 gctacttctg gctgctcggc cgcgtggacg acgtcatgaa cgtgtcgggc catcgacttt
       61 cgacgaccga gatcgagagc gccctcgtct cgcacccggc ggtcgccgag gcggccgtcg
      121 tcggcgggcc ggacgcagtg acgggtgagg cgatcaactg cttcgtgatc ctgcgcaagg
      181 gcgtcgaggc gaccgatgag ctggcactgg agctgcgcaa ccacgtctcg acgaagatca
      241 gcccgatcgc caagccgaaa tccctgatgt tcacgccgga cctgcccaag acccgatccg
      301 gcaagatcat gcgccggctc ctgcgcgaca tcgcccaggg tcgcgccctc ggcgacacga
      361 caaccctcgc cgacgccagc gtcgtggaga cgatccgcga gaactcgggc aagagcgagg
      421 aatagcgcgg gtgcctcccg cgcgccggca cctcctgccc gccccatctt ctgtgctcgc
      481 caagtccgtg gtttgtgtca ggtgttattc acccgggcgt tcgtcaagct gctggcgccg
      541 ccgcaacaag tttcccgatc gggctcccgc cgcgttgctg acagattcgc gcggtcatga
      601 gcaattggct ggaatgtcgt gacgagtgcc cgctccagta acagatgacg catttcgagc
      661 agcagcgggc cgggtagcaa caggtggggg agcatcagat cggtatgcaa agcgtccagg
      721 cagtcgtctt cgacgtcggg gagaccctcg tcgacgagac gcgcgcctgg tcagcctggg
      781 cggatcacct cggggtgccg cgtctcacct tcatgaccgc gctgggcgca tcgctggctc
      841 gtggcgagag ccaccgcgag gtgttcgagc tgttcgcgcc gggccgcgag atcgatgccg
      901 aataccgccg gctcgtggct gcgcgcgact tcccggtggt cgctcccgaa gacctctacc
      961 cggacgcgct gccgtgcctc gaggccctcg ccgccgatgg gtatcggctc gggatcgcgg
     1021 cgaaccagcc ggccgagacc gcggacgcgc tgcgcgaagc ctcgatcgag gtcgagctga
     1081 tggcgatgtc ggacgcgtgg ggcctgtaca agcccgaccc ggcgttcttt gcccggatcg
     1141 cagaggagct cgcgctcccg ccggcggcga tcgcctacgt cggcgaccgg ctggacaacg
     1201 atgtcggacc cgcgcacgcg gcgggcatga tcgcgatctt cgtccggcga ggcccgtggg
     1261 cctggatcaa cacgccgcgc ggcgaaccgc ccgaagccga cctggtcgtg gagagcctgg
     1321 ccgagctgcc gatcaggctt cggagatcac ctttcggccg aagaggttct tgagcagcga
     1381 cgccgtgtcg cgccggcgtt ccggcatcgg ctctccggcg tggaccgaga tcagccggtc
     1441 ggcgaggtgc tcgaagtcct gcgcggcagg gtggcgcggg aacttgtcga tgagggactt
     1501 gcccgcgttc gccgaccaca cgaagtggag cccggcggag cggatgtggg cgatcgcgtt
     1561 cagcccgacg gtcgcctcca tgtcggcgac ggtcacgcca ctgttggcgc ggttgatgat
     1621 catggccagc cgatcgcgga cgccgacctc gagggcgacc tccttgagct ggatcgcggc
     1681 gcgcatggcc ggcaggtccg gcgtcacggg gacgaggatc cggtcggcga tggcgaagat
     1741 ggcgaggttg acctcgctgt aggacgggtg caggtcgacc acgacggcat cgacgccctg
     1801 gcgggcctcc atgatcgcgt cggcgactcg cccgggcggg agattcgagt ggctcagcgg
     1861 gttcgtcgag agagcgacga ccttgatccc ggagctgtgc gtcgtggcga ggtcgagcag
     1921 ggtttcgtgg gcatagcccg catcctcgtc gatccagctg tcagcagcgg accgaccggc
     1981 gggcaggccg agcgagagcc cgacgtgacc cgtgaccgta tcggcatcga ggagcaggac
     2041 ctgcatcccc ttccggatct gcagcaccgc tgcgaggttc gtcgcgatcg tcgtcttgcc
     2101 gacgccgccc ttcgggttga agaccgcgaa gacgggcgcg ccagtggcgc cccaagcgcc
     2161 atcgaggtgc ccgccggcga gcacggcatc cgtgctcgtc gacgttggtg cctggaggcg
     2221 aatggccacg gcctcgacgc gccagcggat ggcgtcggcg gaccagggcc ggaggatgat
     2281 ctcgtcgatg tcgaggccgg cggcctcgag cccgtcgagc tctgcttcgg tagccgagta
     2341 gatcaccgac agcgcacccg gacgcccctt gcgggcctcc tgcacgcgcg ccaccgcgcc
     2401 ctcgggatcg cccgcgatgt cgacgagggc gatgagggtc ggcgtgctcg gcgagaacgc
     2461 ctcgacgagg ggcgccgacg tggccatcgg gatcgtgtcg aagccagctt cgttcaggac
     2521 ctcgacgacc tcggatcgct ccggttcgga gagcgcgacg acgacggtcg ggcgagtgga
     2581 ttcggtggtg gcgtttgcct gtggcatcac gcagcctcag ccgcgacgac ggtgaagccg
     2641 tcgtccgtcg catcggtcac ctgcgcggcg aagccggtca ggtgcgagag ggcggagtcg
     2701 aggtcgacgc cggggtcgtg gctcactgcg aagacgaaca cgcccgcctc acccgacgag
     2761 acgctcaccg agcgcacgcc atcgagctgt ccgagcgcgc ccttgaaggc ggagatgccg
     2821 gccacgctgg tcaggccgct gacgaacagg cgggtcgaat ccgggccatt gggctccgca
     2881 ggcgtctgca ctgcgtcgct ccggtgggcg gcggtgagga ccgcggtcgg ccagtcctcg
     2941 atgccaagcg gatcgagacc ttcggtcgcc tcggcctcgg cctcggcggc cgcaccggcc
     3001 gcgagcccgt ccggggcgtt caccggggca tccattgggg catccatcga cgcatcggcc
     3061 attgcctcgt cgtccatcga cgcgtcgccg atgaagtccg tggccgcagg cccttcgccg
     3121 cgaaggtccg gcggatccgg cgcctgctcg gccagggtcg cgagcgtcgc ggggtcggtt
     3181 tcggcgagaa gacgctcgaa gaaccgctcc atgtccgcct cgaaggcggc cacggtggtc
     3241 tgcacctgct ccacgagctg gtcgaccgtc gcagcatggc gctcggtctc gccggcgagc
     3301 tcgaccttgc gctggtcgat cttgtcatcg gtttcctggc ggacgcgcgc gatctcgatc
     3361 ttggaccagt cgcggatcga ggtgatgtcc tcttcggagc gcttgcgcag ctcggcgacc
     3421 tcggtggtcg cgcgggcccg gatcgcctcg acgcgggcgg tggcctcggc ctcgagccgt
     3481 gcggtcgtct cgccgcggct ggcgatggca gcctcgcgca tggccttgac gaggccggcg
     3541 acgagggggt tgtcccggcg cggcagccgc tgctcggcca cggcctgggg gttcatgaac
     3601 ggtcggtccg tgccgcgacg gtcggcagcg cgccggtccg tggcgcgccg atcgaaggtc
     3661 ggccagaccc cgttgacgtc cccgttctca tcgggcgggg ccgcggcgga ttcggtgagg
     3721 gactcgtcgg cgtcgttcat cgcggcctct ggcgtttcaa cgggcacatc ggctgcggcc
     3781 ggcgccgcat ccgtggtctt ggatcgcgcg ccgcgggagg gccgggacgg gccatcgccg
     3841 aggtcgtcgc gcttcacgtc gatccgggac tcgagcacgg cagggtcgag caggttcgta
     3901 ccgccctcgt cggggcgcgc ggtcccccac ggaaggcgaa aacccttttt ctggttgctc
     3961 gtcatggctc gatgatcctc ggcgcgtggt agcccggctc attccggtgc cggacagaac
     4021 ggactgtggt ggtcaggtcg cgtctggggt agtgagtcgg tggtactccg ggcgccgccg
     4081 gaggtaccgg tcccctcatg cggccgatcg gatttccaca ggtaccctgt gcaaatgctc
     4141 gaccggacgg cgacgcgcaa ggcatctgcg acggggaccg catcggctcc cgtggccgcg
     4201 cagccgcgcg accggctcgg gcgcgcgctg cacgatctcc ggatctcggt cacggaccgg
     4261 tgcaacttcc gctgcccgta ctgcatgccg agcgaggtct tcggccgcga ctaccagttc
     4321 ctgccccgtg ccgagatcct cagctacgag gagatccacc ggcttgccac ggtgttcgtg
     4381 gggctgggcg tgcggaagct ccgactgacc ggcggcgagc cgaccctccg tcgcggcctg
     4441 ccggacctcg tcgccatgct cgcctcgatc gaggcgcccg accagcagcc gctcgacatc
     4501 gccgtcacga ccaatggctc ggcgctccgc cagctcgcac ggcccctcgc ggacgcgggc
     4561 ctgcggcggg tgaccgtctc gctcgactcg ctcgatgacg aaaccttccg gcgcatgaat
     4621 ggcgtcgact ttccggtcgc aaaggtcctc gacggcatcg atgccgcggt cgaggcgggc
     4681 ctcggtccgg tgaagatcaa cgtcgtcctg aagcgcggcg agaacgacgc cgggatcctc
     4741 gacctcgcgc gctgggcgcg cgacgccggt cacgtcctgc gcttcatcga gtacatggac
     4801 gtcgggacga cgaacggctg gcggctcgat gaggtcgtgc cggccgccga ggtcatcgag
     4861 acgatcgggc gcgcctggcc ggtcgagccc gaggcgccga cctatccggg cgaggtcgcc
     4921 gagcgctatc gctacctcga cggcaagggc gagatcggcg tcatcgcctc ggtgacgcga
     4981 ccgttctgcg gcgactgcac gcgggcccgg ttgtcggccg agggcaagct gtatacgtgc
     5041 ctgttcagcg ctgtcggcca cgacctgcgc gacccgctgc gcagtggtgc ctcggatgcc
     5101 gacctcgccg ccggcgtggc cgggatctgg gccgggcgcg acgaccgcta ctccgagcga
     5161 cgcgccgcgg ccaccgaccg gttgccgaag atcgagatgt tcgccctcgg cggctgacct
     5221 cgtgctcggc tcccgacctt ttccacgcac tgtccacatc ggtgcgtatc tggtggaaag
     5281 tgcgccgccg gcacgggtga aagtggtgga taacccggtg gacaccgcac ccactcggac
     5341 ggcactattt gccttgcccg aaggggccgc caaggttgag ggaccgagat cacatcaagg
     5401 gacctcctgc cagggccggt cgcttcgggc gcttctgtac ccggtcgcga cggcgttcct
     5461 gcgccgtccc cgggcggctc ggcgggacgc gatcggcatc ccggtgtcgg tactcccgtc
     5521 gggcccgcag aagttgcggg cctccggccg tcgacccctg gacgaagctc tggcggcccg
     5581 ctgtcgcgat cgctagcctg agcccatgga tcccgcgaca cccatcccac ccatcgacca
     5641 gcccgcgcgg ctcgacgccc tcgaggccgc cgtgcgcgcc cggaccgacc tggttcccga
     5701 gctcgggatc gtgctcggct cgggcctcgg cgggctcgcc gacgagctcc gggacgcggt
     5761 cgcgatcccc ttcgccgacc tgcccggctg gcctgccgcg accgccccgg gacacgtcgg
     5821 acggctgctg ctcggcaccc tggccggcaa gccggttgtc atgctccagg gccgcttcca
     5881 cctctacgag gggaacgcgc cgggactcgt cgtccagccg gtgctgctgt tcaagcagct
     5941 cggtgccgcg accgtgatcc tcaccaatgc cgccggcggc ctcgacccgt ccttcggccc
     6001 gggcacgctc atggtcatcg ccgaccacat caacctgacc ggccagaacc ccctgatcgg
     6061 cccgaatgcg ccgtcgatcg gtgatcgatt cccggacgtc accgacgcct ggagcccacg
     6121 cctacgctcg aagctccacg ctgcaggcgc ggcagagggg gtcaagctcg ccgaaggcat
     6181 ctacgtcggc ctcacgggtc ccaactacga gacgcccgcc gaggtccgga tgtacgccgc
     6241 gttcggcggg cacgccgttg gcatgtcgac ggtgatggag tgcatcgcgg cgcgctgggc
     6301 gggcctcgag gtgtgcggca tctcgctcgt cacgaacgcc ggcgccggtt acagcggcaa
     6361 gccactgacc cacgaggagg tcctcgcggc gggcgccgag gccgggcctc gcttctcgcg
     6421 ggtgatccgc cggttcatcg ccgatctcta ggcggcgccg tcactttcgc gcgccgccgc
     6481 gcctcgacga cttgtgcatc ggacaccaac gctcgcgacg agcgcgattg cgctgaccct
//
LOCUS       DATBSW010000015         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_917827, whole genome shotgun sequence.
ACCESSION   DATBSW010000015 DATBSW010000000
VERSION     DATBSW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917827"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VIF63_00450"
     CDS             complement(<1..740)
                     /locus_tag="VIF63_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8237881.1"
                     /translation="MAAAAAGDGAPAIGAPAEETILVEVDTGARVATITLNRPDALNS
                     LTVPMKQALLAALRRVERDASVRAVILTGAGRAFCAGQDLRERLSPDAAPLGVEVRER
                     YNPIIRAMRGLPKPIVAAINGVAAGAGASLAIASDIRIASDAATFALAFGRVGLVPDS
                     GATWLLPRLVGLTRATEMALLNEPVPAADALRLGLVGRVVPADQLGAEARSVAARLAA
                     GAPRAIALTKRALNAAWDRDLDAALEYEA"
     gene            complement(743..1414)
                     /locus_tag="VIF63_00455"
     CDS             complement(743..1414)
                     /locus_tag="VIF63_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237882.1"
                     /translation="MSISGHQPPVAAPVSHSETPEHDWSAAKVRIWPSLRPAGTHGAH
                     LDDLTEERLLHEGTKKRALPVVDPGPAGLSIVYVLREPAYDVVVNAEHLLTWAVDAQT
                     LRDTAMGNLRTWSESAPWTDELADGRHIVSSDTGDGGDAARILLPEVRQHLAGACGGP
                     GRVLVALPDRDLLIAGSLLPGDESFAEQFAAFAADVYDAAHEPIERHLFELVGAECEL
                     AQFGG"
     gene            complement(1414..2232)
                     /locus_tag="VIF63_00460"
     CDS             complement(1414..2232)
                     /locus_tag="VIF63_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8237883.1"
                     /translation="MTTTEQPAHPTLHVTLPAPSADGPVDGVALVVMDRHEVLNALDF
                     ALIAALTDALEALDRNPDCRAIVLTGAGDRAFAAGADITELAAQSPTSLTVDDDFHRW
                     ERLKRIRKPIVAAVRGFALGGGCELAMLCDLIVAGEDAQFGQPEIKLGVMPGAGGTQR
                     LTRAIGKAKAMELILTGRTMGAREAEAHGLVTKVVPSEATVPAALELAGRIAAMPPVA
                     VMAAKAAVNRAEELALDAGLEFERRNFYLLFDTEDQAEGMAAFTEKRAPRWKGR"
     gene            complement(2259..2600)
                     /locus_tag="VIF63_00465"
     CDS             complement(2259..2600)
                     /locus_tag="VIF63_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEY8237884.1"
                     /translation="MTTLLVLYRAPEGGADALATLESRYRDEHLPLVAETPGLRATRI
                     WRVGGALGGETDLVLAAAMDFDDRAAMDDGLRSDPMRAAGRILRDIAPGLATFLVLED
                     APDLLEGKVGG"
     gene            complement(2597..3439)
                     /locus_tag="VIF63_00470"
     CDS             complement(2597..3439)
                     /locus_tag="VIF63_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8237885.1"
                     /translation="MPTIRANGLDIGYDVAGAGPPLVALHGATSIGREDYAAQLPGLS
                     KAFLVHLPDARGHGRTRWDAANGFEYRWLVDDLTAFVDGLGLDSFHLLGFSMGAMTAL
                     QFAARMPERLRTLVVVGITTHREPRASVGRALMDPVAILARDPTFAATLSRRHDEGQG
                     AGAWQRLLPAIAADIATQPLLAPPQLHGIDCPAMVCCGDRDPFVPTEHAAGLARQLPG
                     GRLFIAPDCGHEVMSRKPGLFNEATAGFYRATEAVARERAARVETVGAPVAGTGARTE
                     GGAG"
     gene            complement(3440..>3961)
                     /locus_tag="VIF63_00475"
     CDS             complement(3440..>3961)
                     /locus_tag="VIF63_00475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8237886.1"
                     /translation="DAFRQVMGHFATGISVVTTWDGEKPAGITVNAFSSVSLEPALVM
                     VALDRRRFITPMVRTAGRYAVNVLGADQQAMSDCFAHAPVRPGEPDREAFCGAAWQSG
                     PTGLPLIDDALATLECTIVETFSAGDHDLFIGRVDSLQQHRDGVAPLLYYRRRYLRIE
                     QGAESPVEGKPEG"
BASE COUNT          552 a         1443 c         1436 g          530 t
ORIGIN      
        1 gcctcgtact cgagcgcggc gtcgaggtcg cggtcccacg cggcgttgag ggcgcgcttc
       61 gtcagcgcga tggcgcgcgg cgcgccggcc gcgagacggg cagcgacgga tctcgcctcc
      121 gcgcccagct ggtcggcggg cacgacccgg cccacgaggc cgagccgcaa tgcgtccgcg
      181 gccggcacgg gctcgttgag cagtgccatc tcggtggcgc gggtcagccc gacgagccgc
      241 ggcaggagcc aggttgcacc gctgtccggc acgagcccga cacgcccgaa cgccagcgcg
      301 aaggtcgcgg catcggatgc gatccggatg tccgaggcga tcgcaagcga ggcgcctgcg
      361 ccggccgcga caccgttgat cgcggcgacg atgggcttgg gcaggccgcg catcgcccgg
      421 atgatcgggt tgtagcgctc gcgcacctcg accccgagcg gcgcggcatc gggcgacagg
      481 cgctcgcgca ggtcctggcc cgcgcagaag gcgcggccgg cgccggtcag gatcacggcc
      541 cgaaccgagg cgtcacgctc gacccgtcgc agcgccgcga gcagggcttg cttcatcggc
      601 acggtgagcg agttgagcgc gtcgggccgg ttgagggtga tcgtggcgac gcgcgccccc
      661 gtgtcgacct cgacgaggat cgtctcctcg gccggcgccc cgatggccgg cgccccgtcc
      721 cccgccgccg cagccgccat cgtcagccgc cgaactgcgc gagctcgcac tcggcgccga
      781 cgagctcgaa caggtggcgc tcgatcggct cgtgcgcggc gtcgtagaca tcggcagcga
      841 atgccgcgaa ctgctcggcg aacgactcgt cgcccggcag cagggaaccg gcgatcagca
      901 ggtcccgatc gggcagggcc acgaggacac ggccaggacc gccgcacgcg cccgcgagat
      961 gctgccgaac ctcgggcaga aggatccgtg ctgcatcacc accatcgccg gtgtccgacg
     1021 agacgatgtg ccggccatcg gccagctcgt cggtccaggg cgccgattcg gaccaggtgc
     1081 gcaggttgcc cattgccgta tcgcgcagcg tctgggcatc taccgcccac gtcagcaggt
     1141 gctcggcgtt gaccacgacg tcgtacgcag gctcgcgcag gacgtagacg atcgacaggc
     1201 cggccggccc gggatcgacg acgggcagcg cgcgcttctt cgtcccctcg tgcaggagcc
     1261 gctcctcggt caggtcgtcg aggtgtgcgc cgtgcgtccc ggccggccgg agcgacggcc
     1321 agatccgcac cttcgctgcc gaccagtcgt gctccggggt ttcgctgtgc gaaaccggcg
     1381 ccgccacggg tggctgatgc ccgctgatcg acatcagcgg cccttccagc gcggcgcgcg
     1441 cttctcggtg aacgcggcca tgccctcggc ctggtcctcg gtgtcgaaca gcaggtagaa
     1501 gttgcgccgc tcgaactcga gcccggcgtc gagcgccagc tcctcggcgc gattgaccgc
     1561 ggccttggcg gccatcacgg cgacgggcgg catcgcggcg atgcgtccag cgagctcgag
     1621 tgccgcgggc accgtcgctt ccgaaggcac gaccttggtc acgaggccgt gcgcctcggc
     1681 ctcacgcgcg cccatggtcc ggccggtgag gatgagctcc atcgccttcg ccttgccgat
     1741 cgcccgcgtc agtcgctgcg tcccgccggc gcccggcatc acaccgagct tgatctcggg
     1801 ctgcccgaac tgcgcgtcct cgccggcgac gatcaggtcg cacagcatcg ccagctcgca
     1861 gccgccgccg agcgcaaacc cgcgaaccgc ggccacgatc ggcttgcgga tccgcttcag
     1921 gcgctcccag cggtggaagt cgtcatcgac cgtcaggctg gtcggactct gggcggcgag
     1981 ctcggtgatg tcggcgccgg ctgcaaacgc ccgatcgccc gcgccggtga gcacgatggc
     2041 ccgacagtcg gggttgcgat cgagcgcctc gagggcgtcg gtgagggcgg cgatcagcgc
     2101 gaagtcgagc gcgttcagga cctcgtgccg gtccatgacc acgagcgcca cgccgtcgac
     2161 cgggccgtcg gcgctgggcg ccggcagcgt gacgtggagc gtcgggtggg cgggctgctc
     2221 ggtggtcgtc acggtgtgcc gccagtatct acggcaacct agccgccgac cttgccttcc
     2281 agcaggtccg gcgcgtcctc gagcaccagg aacgtcgcca ggcccggcgc gatgtcacgc
     2341 aggatccggc cggcggcgcg catcgggtcg gagcggaggc cgtcgtccat ggcagcgcgg
     2401 tcgtcgaagt ccatcgccgc ggcgagcacg aggtccgtct cgccgccgag cgcgccgccg
     2461 acacgccaga ttcgggtcgc ccgcagtccg ggcgtctcgg ccacgagcgg caggtgctcg
     2521 tcgcggtagc gagattcgag cgtggccagg gcgtcggcgc cgccctcggg cgctcgatac
     2581 agcacgagca acgtggtcat ccggctcctc cttcggtacg agcgcctgtg ccggcgactg
     2641 gggccccaac cgtctcgacg cgggcggcgc gctcgcgcgc gacggcctcc gtggcacggt
     2701 agaacccggc ggtcgcctcg ttgaacagac ccggcttgcg cgacatcacc tcatgcccgc
     2761 agtcgggcgc gatgaacagc cggccgcccg ggagctggcg cgccagaccc gccgcgtgct
     2821 cggtcggcac gaatgggtcg cggtcgccac agcagaccat cgccgggcag tcgatgccgt
     2881 gcagctgagg gggcgcgagc agcggctgcg tcgcgatgtc cgccgcgatc gccggcagca
     2941 ggcgctgcca cgcgccggcc ccctggccct cgtcatgtcg ccgcgagagc gtcgccgcga
     3001 acgtcggatc gcgggccagg atcgccacgg ggtccatgag cgcccggccg acgctcgcgc
     3061 gcggctcgcg gtgcgtcgtg atgccgacga cgacgagcgt cctgagccgt tccggcatcc
     3121 gtgccgcgaa ctggagcgcg gtcatcgcgc ccatcgagaa cccgaggagg tggaacgagt
     3181 cgagccccag gccgtcgacg aatgccgtca ggtcgtcgac cagccagcgg tactcgaacc
     3241 cgttcgcggc atcccagcgc gtccggccgt gcccgcgggc gtccggcagg tggacgagga
     3301 aggctttcga cagtccgggc agctgcgccg cgtagtcctc gcggccgatc gaggtcgcgc
     3361 cgtgcagggc cacgagcggc ggcccggcgc cggccacgtc gtagccgatg tcgaggccgt
     3421 ttgcccggat cgtcggcact cagccctccg gcttgccttc gaccgggctt tcggcgccct
     3481 gctcgatgcg caggtagcgg cgccggtagt acagcagcgg cgcgacccca tcccgatgct
     3541 gctggaggga gtcgacgcgg ccgatgaaca ggtcgtggtc gcctgcgctg aacgtctcga
     3601 cgatcgtgca ctcgagggtg gcgagggcgt cgtcgatgag cggcaggccc gtcggccccg
     3661 actgccacgc cgccccgcag aacgcctcgc gatccggctc cccgggccgc accggcgcat
     3721 gcgcgaaaca gtcggacatg gcctgctgat ccgcgccgag cacgttcacc gcgtaccgac
     3781 ctgccgtgcg gaccatcggc gtgatgaagc ggcggcgatc cagcgcgacc atcaccaggg
     3841 caggctcgag ggacacggac gagaacgcat tcacggtgat cccggcaggt ttctcgccgt
     3901 cccaggtcgt caccacgctg atcccggtcg cgaagtgccc catgacctgg cggaaagcgt
     3961 c
//
LOCUS       DATBSW010000016         8272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_424234, whole genome shotgun sequence.
ACCESSION   DATBSW010000016 DATBSW010000000
VERSION     DATBSW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424234"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="der"
                     /locus_tag="VIF63_00480"
     CDS             <1..1138
                     /gene="der"
                     /locus_tag="VIF63_00480"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8237887.1"
                     /translation="IIVGEADGEDSADDDSGGDSAPAEDEAGRKWDAMMAADSDDEPP
                     AIALVGRPNVGKSSLLNALLGHERMIVSDIPGTTRDAIDTRLPWGRTEIVLIDTAGIR
                     RRGRVAGGADADRFSTLRSFRAISRADVAVLLVDAVDGLTAQDMHIAGFVVDEGRGLV
                     IAVNKWDAVEEKTGTTFEQYVEWIRYEAPFLDFAPIVSISAKTGQRVTRVLDLAVDAW
                     AERRRRISTGELNRIVGAATARQEPPAVKGRRPKLFYATQVAVAPPTFVFFAREAQSV
                     HFSYRRYLENQLREHFSYLGTPIRLVFRERATIREPRPLQGARGGSRGKKAAAASRAN
                     GSSSASKRPASPAGKAGTGAKAAPGARTAAGAKARPPSGRRTAR"
     gene            1135..2193
                     /locus_tag="VIF63_00485"
     CDS             1135..2193
                     /locus_tag="VIF63_00485"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY8237888.1"
                     /translation="MTEETGTARVAVVGTGAWGTTLALLVARNEPVTLFGHSAETAAQ
                     IAKSRRNQKRLPGMELPANVHVTADAGVLTLAADLVIVAVPSSHLRSMMERVGRHIAP
                     TADVLSVVKGLENGTLLRMTEVIADAAGIDPARIAALSGPNLAQEIARKLPASAVVAS
                     SDPAVGERIVARLSRRQFRLYTNPDVLGVELCGALKNIVAIAAGAADGLGFGNNGKAG
                     LMTRGLAEMTRLGIAVGANPLTFAGLAGLGDVIATCGSKLSRNHRLGEELANGRSWAE
                     LEAVLPGVAEGAYTVDAALALAARHGVEMPIAQEVHNALFEGKSVQRCLIDLLSRESK
                     DELADFGRWIERMATPPG"
     gene            complement(2190..3815)
                     /locus_tag="VIF63_00490"
     CDS             complement(2190..3815)
                     /locus_tag="VIF63_00490"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8237889.1"
                     /translation="MREQLRVVITVARDATLARVELAYLGFNMAVFGTWVAILVYAYG
                     LGGAGATAVVALVQLVPSGLVAPFAAYAGDRFPRERVLVAGFLWQAASCAATAVALFG
                     NWSPVLTITFATIASASFTITRPTLFVILPGITHTPGDLTAANAVSGLVENVARFLGP
                     LVGGLVLAAANAGAVFAVFAIVSLGSAVLVSRLPTQLQRSATLAGSGVRAILAGSFGG
                     FGILARDRALLLLVTVLSLAVAVNGALDILFVATAIDLLHAGEAWAGYLTAAFGLGGV
                     IGAVATIVLIGRRRMTPALATSGTLLGAPIAAVSVLTSTLLAPLLFAVSGAGYSINAV
                     AGRTLLQRTTPEALLARVFGVVESLGLFALGLGSLAAGIIATTAGTRWGLVVVGLLVP
                     VTLVLVWRKLAALDRDARELDVEALALLRRSAIFAPLSALTIERILAELTRIEVPAGH
                     VLIQQGDPGDRFYVVAEGRVQVIRDGTVVAERTTGDHFGEIALLRDVPRTATVTALTP
                     LRVIAIERDRFLEAVTGHSQSREHVEAIAAERS"
     gene            3902..3978
                     /locus_tag="VIF63_00495"
     tRNA            3902..3978
                     /locus_tag="VIF63_00495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3936..3938,aa:Pro,seq:ggg)
     gene            4069..4614
                     /locus_tag="VIF63_00500"
     CDS             4069..4614
                     /locus_tag="VIF63_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237890.1"
                     /translation="MNDPADALESLTKMRNRRLITEQEFQAKRTEIMARLTPALPPPV
                     AHVAHVAPEPTKPEPVRKAPPREARPAAATKPPRAPTPGQQGQRMVRKYQARSEARAE
                     QLMANDREAAGYQVEARRWRAASPIRIVVPPAILLVAGYVLAETAGLVIAAIFGILYM
                     LYLIAAGGVLTVTYRRSAGPR"
     gene            4611..5090
                     /locus_tag="VIF63_00505"
     CDS             4611..5090
                     /locus_tag="VIF63_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8237891.1"
                     /translation="MTGEALELRPLRDEEYDDFSAQTTASYAQEISELGDTPLDVARR
                     RAHESMREILPDGLATPGHWISVLELDGQRVGLLWLATRTIDERQVMFIYDVEIDEAY
                     RGRGLGRKAMLLAERETLAQGLHRIELNVFGGNAVARKLYLSLGYVERAVHMAKDLD"
     gene            5114..5755
                     /locus_tag="VIF63_00510"
     CDS             5114..5755
                     /locus_tag="VIF63_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8237892.1"
                     /translation="MPDEPCACGCPNTFDAKMAEGDLKRYLKQGPDDTTRSLIDAIVG
                     EGIQGATLLDIGGGIGAIQLELLAAGATSVTAVDATEAYIQVARAEAIRRGYGERVRP
                     LIGTFESLASEIEPADVVTLDKVVCCDPNLPGLLGAVATHASRMVGLVYPRVTWWNKI
                     AARVLAAWGWLTRDSIRWHLHANADIDGILRRAGFVRRDIDRTLIWQVALYVR"
     gene            complement(6029..7099)
                     /locus_tag="VIF63_00515"
     CDS             complement(6029..7099)
                     /locus_tag="VIF63_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8237893.1"
                     /translation="MSRFMRPDGIATRIVLAALVVVSIALAIMAIGVMRVGSETFMEL
                     MHALGAPTDEAASMFQHSVTEILAVAAVVAAIAALGVGMWLARRIARPIERLAEAAQR
                     TAEGDLRATAPVEGPAEVRALANAFNVMVDRLAEQDAVRREFVVNASHELRTPLTNLQ
                     GYLEALRDGVLPPDPQTFDSLREEVDRLTRLASSLDLLAGADDERPMPVDVDLGALVR
                     ASLELTAPSFARRSIAVDSSIAPGIVVHARGDELAQVVSNLLQNALRYTPSGGEVRVT
                     LATDAGHAQVRVSNSGSEIPAEDLPRVWERFFRVERSRDRASGGAGIGLAIVKRLIEE
                     AGGRVGASSEAGVTTFWFSLPS"
     gene            complement(7096..7854)
                     /locus_tag="VIF63_00520"
     CDS             complement(7096..7854)
                     /locus_tag="VIF63_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8237894.1"
                     /translation="MAAPDAPILVVDDDPKIVTLVRTYLERERYPVITAADGPSALRA
                     IETLAPRLVVLDLMLPGMDGLAVIRRARALGETPILVLSARGSTGDKILGLSEGADDY
                     LAKPFSPAELVVRVRTILRRTERPLGDALAPDELLVRGDLVVDAGRHEARIAGRPITL
                     SALELRLLATLLDADGRALTRDQLLDGIHGSSEGDVLDRAIDVYIKRLREKLGDDAND
                     PRYVATVRGVGYRAAGRVERRRSGGSSGSGGEAS"
     gene            7958..>8272
                     /locus_tag="VIF63_00525"
     CDS             7958..>8272
                     /locus_tag="VIF63_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEY8237895.1"
                     /translation="MPPIDELIRIEPIYLDRLQRQGIFTTGLLLEVTETPTRRQTLAD
                     QVDASTNDVLEWRDEAQMLNLAGFGPAEHRLLSQSGIEGLKDILEVDLETFGARLARG
                     AME"
BASE COUNT         1314 a         2867 c         2946 g         1145 t
ORIGIN      
        1 gatcatcgtc ggcgaggccg atggcgagga ctcggccgac gacgattccg gcggcgattc
       61 cgcaccggcc gaggacgagg ccgggcggaa gtgggacgcg atgatggcgg ccgactcgga
      121 cgacgagccg ccggccatcg cgctcgtcgg gcggccgaac gtgggcaagt cgagcctgct
      181 caacgcgctg ctcggccacg agcggatgat cgtgtccgac attccgggca cgacccgtga
      241 cgccatcgac acgcgactgc cgtggggccg caccgagatc gtgctcatcg acacggcggg
      301 gatccggcgc cggggccggg tcgccggtgg cgccgacgcc gaccggttct cgacgctgcg
      361 gtcgttccgc gcgatctctc gcgccgacgt cgcggtgctg ctcgtggatg ccgtagacgg
      421 gctgacggcg caggacatgc acatcgccgg attcgtcgtc gatgagggcc gcggcctcgt
      481 gatcgcggtc aacaagtggg acgccgtcga ggagaagacg ggcacgacgt tcgagcagta
      541 cgtcgagtgg atccgctacg aggcgccgtt cctcgacttc gcgccgatcg tgagcatctc
      601 ggcgaagacc ggccagcgcg tgacccgcgt tctcgacctg gcggtcgacg catgggcgga
      661 acgccgccgg cgcatctcga ccggcgagct gaatcggatc gtgggcgccg ccaccgcccg
      721 ccaggagccg ccggcggtga agggccggcg cccgaagctg ttctacgcga cccaggtcgc
      781 cgttgcgccg ccgacgttcg tgttcttcgc tcgcgaggcg cagtcggtcc acttcagcta
      841 tcggcgctac ctcgagaacc agctgcgcga gcacttcagc tacctcggca ccccgatccg
      901 gcttgtcttc cgcgagcgcg cgaccattcg cgagccgcgc cccctgcaag gggcccgagg
      961 cggatctcgt gggaagaagg cggccgcagc ctcgcgcgcc aacgggtcca gctcggcctc
     1021 gaagcgaccg gcctcgccag ccggcaaggc aggcaccggc gcgaaggccg ctcctggcgc
     1081 caggactgct gccggcgcga aggccagacc gccatccggg cgtcgaacgg ctcgatgacc
     1141 gaggagaccg gcaccgctcg ggtcgcggtg gtcggcaccg gcgcatgggg gacgacgctc
     1201 gcgctgctcg tcgcgcgaaa cgagccggtc accctgttcg gccactcggc cgagacggcg
     1261 gcgcagatcg cgaagtcgcg gcgcaaccag aagcggctcc cgggcatgga gcttccagcg
     1321 aacgtccatg tgaccgcgga tgctggcgtg ctcaccctgg cggccgacct cgtgatcgtc
     1381 gcggtgccgt cgtcgcacct ccgatcgatg atggagcggg tgggccggca catcgcgccg
     1441 accgccgacg tgctgtcggt ggtcaagggc ctcgagaacg ggacgctgct ccggatgacc
     1501 gaggtcatcg ccgatgccgc gggcatcgat ccggcgcgaa tcgctgcgct gtccggcccg
     1561 aatctcgccc aggagatcgc gcgcaagctg ccggcgtccg cggtggtcgc ttcgagcgat
     1621 ccggccgtcg gcgagcgcat cgtcgcccgc ctcagccgcc gccagttccg gctgtatacg
     1681 aaccccgacg tcctcggcgt cgagctgtgc ggggcgctca agaacatcgt cgcgatcgcg
     1741 gccggggccg ccgacggtct tgggttcggc aacaacggca aggccgggct gatgacgcga
     1801 ggtctcgccg agatgacccg gcttgggatc gccgtcggcg cgaatccgct gacgttcgcg
     1861 ggcctggccg gccttggcga cgtgatcgcc acgtgcggct cgaagctgtc gcgcaaccat
     1921 cgactcggcg aggagctggc gaacggccgc tcgtgggccg agctcgaagc ggtcctgccg
     1981 ggcgtggccg agggcgccta cacggttgat gcagcgcttg cgcttgcggc ccgccacggc
     2041 gtcgagatgc cgatcgcgca ggaggtccac aacgccctgt tcgaaggcaa gagcgtccag
     2101 cgctgcctga tcgacctgct gtcgcgagag tcgaaggacg agctggccga cttcggccgg
     2161 tggatcgagc ggatggccac gccgcccggc tagctgcgtt cggcggcgat cgcctcgacg
     2221 tgctcccggc tttgcgagtg ccccgtgacg gcctcaagga atcggtcgcg ctcgatcgcg
     2281 atgacccgca gcggcgtcag ggcggtcacc gtcgcggtgc gtggcacgtc gcggagcagg
     2341 gcgatctcgc cgaagtggtc gcccgtggtg cgttcggcga caacggtgcc atcgcggatg
     2401 acctggacgc gaccctcggc gacgacgtag aagcgatcgc ctggatcgcc ttgctggatc
     2461 aggacatggc ctgcaggcac ctcgatcctg gtcagctcgg caaggatccg ttcgatcgtc
     2521 agcgccgaca ggggcgcgaa gatcgccgat cgtcggagga gcgcgagtgc ctctacgtcg
     2581 agctctctgg catcgcggtc gagcgccgcc agcttcctcc agacgaggac taatgtcacc
     2641 ggaacgagca gcccgacgac aacgaggccc catcgggtcc cggccgtcgt cgcgatgatc
     2701 cctgccgcga gcgagccgag cccgagggcg aagagcccga ggctctcgac gacgccgaac
     2761 actcgggcga ggagcgcctc cggcgtcgtg cgctggagca gcgtccggcc tgccaccgcg
     2821 ttgatgctgt agccggcccc gcttaccgcg aacagcagcg gcgccaacaa cgtgctggtg
     2881 aggacgctca cggcggcgat cggtgcgccc aggagcgtcc cgctcgtcgc cagtgccggg
     2941 gtcatgcgcc gtcgaccgat caggacgatc gtcgcgaccg ctccgatcac accgcccagc
     3001 ccgaacgccg ccgtgaggta gcccgcccac gcctctccag cgtggagcag gtcgatcgct
     3061 gttgcgacga acaggatgtc gagtgcgcca ttgaccgcga cggcgagtga gagcacggtc
     3121 acgagcaaca gcaacgctcg atcccgagcg aggatgccaa accccccgaa cgatcccgcg
     3181 aggatggctc ggacgccact cccggccagc gtcgccgagc gctggagctg ggtgggcagc
     3241 cgcgacacca gtacggcgga cccgagcgac acgatggcaa agaccgcgaa tacggcgccg
     3301 gcgttggcag ccgcgagcac gaggccgccg acgaggggcc cgaggaaccg ggcgacattc
     3361 tcgacgaggc ccgagacggc gttggcagcc gtgaggtcgc ccggggtgtg ggtgatgccc
     3421 ggcaggatca cgaagagcgt cggccgcgtg atcgtgaagc tcgcggacgc gatcgttgcg
     3481 aaggtgatcg tcaggactgg cgaccagttg ccgaacaggg cgacggccgt tgctgcacac
     3541 gaagccgcct gccacaggaa gcccgcaacc aggacccgtt cccttggaaa cctgtcaccg
     3601 gcatacgcgg cgaagggagc aacgagaccc gatggcacga gctggaccaa cgcgacgact
     3661 gcggtcgcgc cggcgccccc gagcccatag gcgtacacga ggatcgcgac ccaggtcccg
     3721 aacacggcca tgttgaagcc gaggtacgcg agctcgacgc gtgcgagcgt ggcgtcacgc
     3781 gccaccgtga tcacgacgcg cagctgctcc cgcatctgcc ggccagcatg acagtgcgcc
     3841 gatcgccgaa tctggccccg gcggagcgtc gaagggtggc tgctagactc gacgttcgcg
     3901 tcggggcgtg gcgcagcctg gtagcgcacc agtctggggg actggtggtc ggaggttcaa
     3961 atcctctcgc cccgaccatt tctcccgtcc aagattgatc ccgaacccac gattgtcggg
     4021 ttcgatgcga agaaggcgga actcctggcc cgtgtctgac gcgagcgaat gaacgaccct
     4081 gccgacgccc tcgagtcgct cacgaagatg cgcaaccgcc ggctcatcac cgagcaggag
     4141 tttcaggcca agcgcaccga gatcatggcc cggctgacgc ccgcgctccc tccgcctgtc
     4201 gcacatgtcg cacatgtcgc gccggagccc acgaagccag agcccgtcag gaaggcacct
     4261 ccgcgcgaag ccaggcctgc cgccgccacg aagccgccgc gcgcgccgac gcccgggcag
     4321 caggggcagc ggatggtgcg caagtaccag gcgagatccg aggcgagggc cgagcagctg
     4381 atggccaacg accgcgaagc cgccggctac caggtcgagg cgcggcgctg gcgggcggcg
     4441 agcccgatca ggatcgtggt cccgccggcg atccttctcg tcgcggggta cgtgctggct
     4501 gagactgccg ggctcgtcat cgccgccatc ttcgggatcc tgtacatgct gtatctcatc
     4561 gcagccggcg gagtcctcac cgtcacctat cggcgttcgg caggccccag atgacggggg
     4621 aggccctcga gcttcgaccg ctgcgcgacg aggaatacga cgacttctcc gcgcagacca
     4681 ccgcgtcgta cgcccaggag atcagcgagc tcggcgacac gccgctcgac gtcgcccggc
     4741 gccgggccca cgagagcatg cgcgagatcc tgcccgacgg gctcgccacg ccgggtcact
     4801 ggatcagtgt cctcgagctc gatggtcagc gcgtcgggct gctctggctt gccacccgga
     4861 cgatcgatga gcgccaggtc atgttcatct acgacgtcga gatcgacgag gcgtatcgag
     4921 ggcgcgggct tggccggaag gcgatgctcc tcgccgaacg cgagacgctg gcgcagggcc
     4981 tccatcggat cgagctcaac gtgttcggcg ggaacgcggt cgcccgaaag ctgtacctgt
     5041 cgctgggcta cgtcgagcgc gcggtgcaca tggccaagga cctcgactga cccaacctcg
     5101 gcatactgcg cccatgcccg acgaaccctg tgcctgcggt tgcccgaaca ccttcgacgc
     5161 gaagatggcc gagggcgacc tcaagcgcta cctcaagcag gggcctgacg acacgacgcg
     5221 atccctgatc gacgcgatcg tcggggaggg gatccagggc gcgacgctgc tcgacatcgg
     5281 gggcgggatc ggggcgatcc agctcgagct gctcgcggcc ggcgccacat ctgtgacggc
     5341 cgtcgacgcc accgaggcgt acatccaggt cgcccgggcc gaagcgatcc gacgcggcta
     5401 cggcgagcgg gtgcgaccgc tgatcgggac gttcgagtcg ctggcgtcgg agatcgagcc
     5461 cgcggacgtc gtcacccttg acaaggtggt ctgctgcgat ccgaacctgc cgggcctgct
     5521 cggtgccgtc gcgacccatg cctcccggat ggtcgggctc gtctatcccc gggtcacgtg
     5581 gtggaacaag atcgccgccc gcgtcctggc cgcctggggc tggctgaccc gtgactcgat
     5641 ccgctggcac ctccacgcga acgccgacat cgacgggatc ctgcgacggg ccggcttcgt
     5701 gcgccgggac atcgaccgaa cgctgatctg gcaggtcgcg ctgtacgtca gatagcgcgc
     5761 gcgattcgcc gcgtgggcgt cctcactcga tcgcggcgtg ggcttgcctc gacggcgacc
     5821 gctcggcgat ccggggcgag gcgcaatagt tcgaggaagc tccgacgagc acggaatggg
     5881 cgctcgtcga ggtgcgctcg accctcggcg ctgccagcgc cgcggccggt cgcgaggaac
     5941 caatcaagct tgccgggcat gcggcaatcg tggtccttga cgggttgcgg acgccttgca
     6001 aagggccacg ctcggttgca ccgagccgtc agctcgggag cgagaaccag aacgtggtga
     6061 cgcccgcctc cgatgaggcg cccacgcggc cgccggcctc ctcgatcagg cgtttcacga
     6121 tcgcgaggcc gatcccggcg ccgcccgatg cccgatcccg cgaccgttcg acgcgaaaga
     6181 accgctccca gactcgaggc agatcctcgg cagggatttc gctcccgctg ttcgagacgc
     6241 gcacctgggc atggccggcg tcggtggcaa gcgtgacccg cacctcgccg cccgacggcg
     6301 tgtagcgcag cgcattctgg agcaggttcg acacgacctg cgccagctcg tcgccacgag
     6361 cgtggacgac gatcccgggc gcgatcgacg agtcgaccgc gatcgagcgg cgggcgaacg
     6421 acggcgccgt cagctcgagg ctggcccgga cgagggcgcc gaggtcgacg tccacgggca
     6481 tggggcgctc gtcgtcggcg ccggcaagca ggtcgaggga cgaggcgagg cgcgtcaatc
     6541 ggtcgacctc ctcgcgcagc gagtcgaacg tctgtgggtc gggcggcagc acgccgtcgc
     6601 ggagcgcctc gaggtagccc tggaggttgg tgagcggggt ccggagctcg tgcgacgcgt
     6661 tgaccacgaa ctcgcggcgg accgcgtcct gctccgcgag ccgatcgacc attacgttga
     6721 aggcattggc gagggcacga acctccgccg ggccttcgac cggcgcggtg gcgcgaagat
     6781 cgccctcggc ggtccgctgc gctgcctcgg caagacgctc gatcggtcgg gcgatgcgac
     6841 gtgccagcca catgccgaca ccgagcgctg cgattgcggc gaccactgcg gcgacggcca
     6901 ggatctcggt gacgctgtgc tggaacatcg aagcggcttc gtcggtgggc gcgccgagcg
     6961 cgtgcatcag ctccatgaac gtctcggatc cgacgcgcat cacgccgatc gccatgatcg
     7021 ccagcgcgat cgagacaacg acgagcgccg cgaggacgat ccgcgtcgcg atgccgtccg
     7081 gccgcatgaa ccggctcatg acgcttcgcc gccgctgcca ctgctgccgc cgctccgccg
     7141 gcgctcgaca cggcccgcgg cgcggtagcc cacgccgcgc acggtggcga cgtaccgcgg
     7201 gtcgttggcg tcgtcgccga gcttctcgcg gaggcgcttg atgtagacgt cgatcgcccg
     7261 gtcgagcacg tcgccctcgc tcgagccatg gatgccgtcg aggagctggt cccgagtgag
     7321 cgcgcggccg tcggcgtcga gcagcgtcgc gaggaggcgc agctcgagcg cgctgagcgt
     7381 gatcggtcgt ccggcgattc gcgcttcgtg ccggcccgcg tccacgacca ggtcgccgcg
     7441 gacgagcagc tcgtcgggtg cgagcgcgtc gccgagtggg cgctcggtgc ggcgcaggat
     7501 cgtgcgaacg cgcacgacga gctccgcggg ggagaacggc ttcgcgagat agtcgtccgc
     7561 gccctcgctc aggccgagga tcttgtcgcc ggtcgagccg cgcgcggaca ggaccaggat
     7621 cggcgtctcg ccgagtgccc gcgcgcggcg gatcaccgcc aagccgtcca tccccggcag
     7681 catcaggtcc agcacgacca gccgcggggc gagcgtctcg atcgcgcgca gcgcggaggg
     7741 cccgtcggcg gcggtgatga ccgggtaccg ctcgcgctcg aggtacgtcc ggacgagcgt
     7801 gacgatcttg gggtcgtcgt cgacaacgag gatcggggcg tcgggcgcgg ccatgaacag
     7861 catcatcggt cgcccgaggg tcgccgacaa ctcgtgacgc gccgatgacg cctgccctga
     7921 cgggcacttg accggcgagc ggccctatca tcgccgcgtg ccaccgatcg acgagctgat
     7981 ccgcatcgag ccgatctacc tcgaccgcct gcagcgccag ggcatcttca ccaccggcct
     8041 gctgctggag gtcaccgaga cgccaacccg gcgccagacc ctcgccgacc aggtcgatgc
     8101 atcgacgaac gacgttctcg agtggcgtga cgaggcgcag atgctcaacc tggcgggctt
     8161 cgggcctgcc gagcatcgac tgctcagcca gagcggcatc gagggcctca aggacatcct
     8221 cgaggtggat ctcgaaacgt tcggcgcgcg gctcgctcgc ggggccatgg ag
//
LOCUS       DATBSW010000017         6939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_177410, whole genome shotgun sequence.
ACCESSION   DATBSW010000017 DATBSW010000000
VERSION     DATBSW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177410"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VIF63_00530"
     CDS             <1..580
                     /locus_tag="VIF63_00530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY8237896.1"
                     /translation="TVDLAVLGRRERDHRSGFEVAPMPHIDAAPNERETRYGGLDDQR
                     LGGITWDEIGAIRSWSSMPLVLKGILAPDDARRAVDVGVAGIVVSTHGGRQLDRSIAS
                     ADALGPIVDAVAGQTEVWVDGGIRRGLDVVTALCLGATGVLVGRPMYWALAAGGQAGV
                     ERAIAMLRHELELALPLLGAASVGALSRELLT"
     gene            complement(573..1118)
                     /locus_tag="VIF63_00535"
     CDS             complement(573..1118)
                     /locus_tag="VIF63_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8237897.1"
                     /translation="MQIPELDTERLRLRGWRASDLAPFAALNADPEVMEHFPERLDRR
                     SSDALVARIEAAWTENSFGLWAVERRVDGLFIGFVGLSRPSFEAQFTPAIEVGWRLAR
                     GAWGFGYATEAARAAVRYGFEILGLDEIVSFTVPANVRSRQVMERLGMQRDPADDFDH
                     PRLPAGHPLQRHVLYRLRAPT"
     gene            1173..3344
                     /gene="ligA"
                     /locus_tag="VIF63_00540"
     CDS             1173..3344
                     /gene="ligA"
                     /locus_tag="VIF63_00540"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY8237898.1"
                     /translation="MATTEVESTGTDAIGLDGLPVDPALIAEAAALGEEAATARHAEL
                     VREIEDANEAYHVKDAPVITDAEYDQLFRRLVALESAFPALITPESPTQAVGGHLAGT
                     FDEVRHRRPMLSLSNAFSHDELRAFDGRVRRGLGLAAAPEPADDLRYVAELKIDGLAI
                     SLRYERGRFVQGATRGDGTTGEDVTANLRTISTVPARLAEPVSLDARGEVYMPKSEFA
                     RINSERAELGLPKYANPRNSGAGSLRQKDSAVTAARQLATWSYQLIEDEAAGTPELAS
                     QSAALARLAALGFTVNPNHEAGLDIEGVIAFTEAWREKRHGLPYETDGVVVKVDAFEQ
                     QARLGIVSRAPRWAIAYKYPPEQVETVLEDIVAYVGRTGTLTPVAHLRPTKVAGSTVA
                     RATLHNLDELRRKDIRIGDHVILQKAGDVIPEVVGPVVSKRTGKEREWDMPTECPVCG
                     QPVVRDEGAVRHYCANPACPARVGQEFGHFVGSMDIDGLGWAVLSQLLERGLVKRRGD
                     FFRLSIEDLESLDRFARKSAENLHAAIQKARRRPLERLIAALGIPQVGWTTAIELSHW
                     LAEAVPPGDRWLVRAAELLKSTAADDPERFQQLDGVGPTLATALATFFAPGGAGEGVL
                     EDLEDAGVEIELPAPRTAAADGPLAGKQVVVTGTIEGFSREEAEAAVRAAGGKPVGSV
                     SRKTDYVVAGPGAGSKLAKAEELGVAVLDADGFRALLEQGQ"
     gene            3350..3889
                     /locus_tag="VIF63_00545"
     CDS             3350..3889
                     /locus_tag="VIF63_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8237899.1"
                     /translation="MQTPPDQAAPDPVSQAAAYQQMLLAALGDDDPAEVQARTPADAR
                     ALFELAGPDLRTRPEPREWSVLLCIAHMTDGEVVMSARYRWVLAHDEPEFIGYDQDLW
                     ADRLHDGQDPEALLAHFTALRTANLELWRRSSEADKARVGQHRERGPESYDLMFRLMG
                     GHDRVHLAQARRALEAVRG"
     gene            3948..5267
                     /locus_tag="VIF63_00550"
     CDS             3948..5267
                     /locus_tag="VIF63_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY8237900.1"
                     /translation="MDALARGAARPHALAAFVAIGLIAFTALGLSTPQRFAAAEGRTL
                     HVPAEHATLEAAIAASTAGDVILLAAGTYPGNVVVPESRPGITIHGEDRNSVVFDGAD
                     TRANAIEVEADGVTLENMSAHNFTDNGFYWDGVDGFAGRYLTVWNVGLYAIYAIESRH
                     GIIEHSYASGAADAAFYIGECNPCDTTLRDLIAVLSAVGYSGTNAGGNLVIEDSRFEN
                     NGIGILPNSFEVGLEPPPQRDAIVRHNVVIGSGSVPTPRATPLGGYHGIGIGVAGGVG
                     NVIEGNEVTGSARYGIAVFTAVDRETPWVSADNSIKSNQVSSSGLADLALAAGSGANN
                     CFTGNTAAVTLPVDLAASCSVLGAGDPTVAGNLNVAPPRLLEGLPEAPSYATMPAPPV
                     QQTMAVGDATMNDVVLFIAAVVAAGLALVTLLAFGFMRLRRRRTLTG"
     gene            complement(5280..6146)
                     /locus_tag="VIF63_00555"
     CDS             complement(5280..6146)
                     /locus_tag="VIF63_00555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8237901.1"
                     /translation="MATLRPTLRRMLPADVEAATEMILRNDWGVRRDWLSFAATQAAC
                     RPIVAEADGVIVGTGVGTANGAVGWIGTIFLEPEWRGQGLGRALTQAIIDQLEEAGCR
                     TLVLVATQDGRRLYEKMDFRVQTRYRILEAPGLPTGSAGSTTAAAEGPVVRPFNPSDL
                     DFLHALDRAATGEDRRHALERFAAPETAKVAVMPDGSLGGFVIRAPWGGGATVARSPD
                     AATAIVTARRETSGPTGRVRVGILAGNAAGAAKLVGLGFEQRWSAPRMIRGEPLHWHP
                     SWIWGQFNHAMG"
     gene            6169..6468
                     /gene="gatC"
                     /locus_tag="VIF63_00560"
     CDS             6169..6468
                     /gene="gatC"
                     /locus_tag="VIF63_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY8237902.1"
                     /translation="MAGLTRTDVEHVAFLARLRLTDEELVRLEGQLNHILDQYAKLSE
                     LDTSAIPPTAQTIELENILRDDVAGPSLAVEDVLRNARVTEGDFIVVPPILGGDA"
     gene            6548..>6939
                     /locus_tag="VIF63_00565"
     CDS             6548..>6939
                     /locus_tag="VIF63_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY8237903.1"
                     /translation="MLDSALRSTTLAGRLVIDLTRALGHQLAAQLRAGEVSSRELTLA
                     HLDAAERQNHALNAWLVIERDKALAEADAADARLAAARAGGPAAVDALHPLLGLPVAV
                     KDLVSVAGGQCTAGSRILEGYVAPYDAHV"
BASE COUNT         1030 a         2496 c         2436 g          977 t
ORIGIN      
        1 gacggtggac ctcgcggtgc tcggccggcg ggagcgcgac catcggtccg gcttcgaggt
       61 cgccccgatg ccgcatatcg acgcggcgcc gaacgagcgc gagacccgct acggcggcct
      121 cgacgaccag cggctcggcg gcatcacctg ggacgagatc ggtgcgatca ggtcgtggag
      181 ctcgatgcca ctcgtgctca agggcatcct cgcccccgat gacgcgcgcc gcgccgtcga
      241 cgtgggcgtg gccgggatcg tggtctcgac ccatggcggg cggcagctcg accgatcgat
      301 cgcgagcgcc gacgccctcg ggccgatcgt cgacgcggtg gcgggccaaa ccgaggtctg
      361 ggtcgacggc ggcatccgtc gcggcctcga cgtcgtgact gcgctctgcc tcggcgcgac
      421 gggcgtgctc gtcgggcggc cgatgtactg ggcgctcgcc gctggcggcc aggccggcgt
      481 ggagcgcgcg atcgcgatgc tccgccatga gctggagctt gccctgccgc tcctcggcgc
      541 cgcgtcggtt ggcgcgctca gtcgcgagct gctcacgtag gagccctcag gcggtagagc
      601 acgtgccgct gcagcggatg gccggcgggc aggcgcggat ggtcgaagtc gtcggctgga
      661 tcgcgctgca tgccgagtcg ctccatgacc tgccgtgagc ggacgttggc cggcaccgtg
      721 aacgagacga tctcgtcgag gccgaggatc tcgaacccgt acctgaccgc cgccctcgca
      781 gcctccgtcg cgtagccgaa gccccacgcg ccgcgcgcca accgccagcc gacctcgatg
      841 gccggcgtga actgcgcctc gaacgatggt cgagacaagc cgacgaagcc gatgaacagg
      901 ccatccaccc gtcgctcgac cgcccacagg ccgaacgagt tctccgtcca ggctgcttcg
      961 atgcgggcga ccaacgcgtc gctcgaccgc cgatcgaggc gctcggggaa gtgctccatc
     1021 acctcagggt cggcattcag cgcggcaaac ggcgccaggt cggacgcgcg ccagccgcga
     1081 aggcggagtc gctccgtgtc gagctcgggg atctgcatcg cgcgcattgt gcccacgtcg
     1141 ctcgacgcga tcccttcgta tccttcgaac cgatggcgac aaccgaggtg gaatcgactg
     1201 gcaccgatgc gatcgggctc gacgggctgc ccgtcgatcc ggcgctgatc gccgaggccg
     1261 ctgcgctcgg cgaggaagcg gccaccgcgc gccacgctga gctcgtgcgt gagatcgagg
     1321 acgcgaacga ggcgtaccac gtcaaggacg cgccggtgat caccgatgcc gagtacgacc
     1381 agctgttccg acggctcgtc gccctcgaat cggcgttccc cgccctcatc acgcccgaat
     1441 cgccgacgca ggccgtgggc ggccaccttg ccggcacgtt cgatgaggtc cgccaccggc
     1501 ggccgatgct gtcgctctcg aacgcgttct cgcacgacga gctccgtgcc tttgatggcc
     1561 gagtccgtcg cggcctgggg cttgctgcgg cgccagagcc ggcagacgac ctgcgctacg
     1621 tcgccgagct gaagatcgac ggcctcgcca tctcgctgcg ctacgagcgc ggccggttcg
     1681 tccagggcgc gacgcgcggc gacggcacga ccggcgagga cgtcaccgcc aacctgcgca
     1741 cgatctcgac cgtgcccgcc cgtctcgccg agcccgtctc gctcgacgcc cgcggcgagg
     1801 tgtacatgcc caagtcggag ttcgcgcgga tcaactcgga gcgggcggag ctgggcctgc
     1861 cgaagtacgc gaacccgcgg aactccggcg ctggctccct gcgccagaag gactccgcgg
     1921 tcacggctgc ccggcagctc gcgacgtggt cgtaccagct gatcgaggac gaagccgccg
     1981 ggacgcccga gctggcaagc cagtcggcgg cgctcgcccg gctcgctgcc ctcggcttca
     2041 ccgtcaaccc gaaccacgag gcgggcctcg acatcgaggg cgtcatcgca ttcaccgaag
     2101 cgtggcgcga gaagcgccac gggctgccgt acgagaccga tggggtggtg gtcaaggtcg
     2161 atgcgttcga gcagcaggcg cggctgggca tcgtcagccg cgcgccgcgc tgggcgatcg
     2221 cctacaagta cccacccgag caggtcgaga cggtgctcga ggacatcgtg gcgtacgtcg
     2281 ggcgcaccgg gacgctcacg cccgtggcgc atctccggcc gacgaaggtc gcgggctcca
     2341 cggtggcgcg cgccacgctc cacaacctcg acgagcttcg gcggaaggac atccggatcg
     2401 gcgaccacgt gatcctccag aaggccggcg acgtcatccc cgaggtggtg ggcccggtcg
     2461 tgtcgaagcg caccggcaag gagcgcgagt gggacatgcc caccgaatgc ccggtgtgcg
     2521 gccagccggt cgttcgcgac gagggcgcgg tccggcacta ctgcgcgaat cccgcgtgcc
     2581 ctgcccgggt cgggcaggag ttcggccact tcgtcggcag catggacatc gacggcctcg
     2641 gctgggcggt gctcagccag ctgctcgagc gcggcctcgt caagcggcgc ggcgacttct
     2701 tccggctttc gatcgaggat ctcgaatcac tggaccggtt cgcgcgcaag agcgcggaga
     2761 acctccacgc ggcgatccag aaggcgcgcc gccgcccgct cgagcgcctg attgccgcgc
     2821 tgggcattcc ccaggtcggc tggacgacgg cgatcgagct cagccactgg ctcgccgagg
     2881 cggtgccgcc cggcgatcgc tggctcgtgc gggccgccga gctcctcaaa tcgaccgctg
     2941 cggacgatcc ggagcgattc cagcagctgg acggcgtcgg cccgacgctc gcgaccgcgc
     3001 tggccacgtt cttcgcgccc ggtggcgccg gcgagggcgt gctcgaggat ctcgaggacg
     3061 ccggcgtcga gatcgagctg cccgcgccac ggaccgccgc cgccgacggc ccactcgccg
     3121 gcaagcaggt cgtggtcacc ggcacgatcg aagggttcag ccgcgaggaa gcggaggcgg
     3181 cggttcgcgc ggccggcggc aagccggtgg gctcggtgtc gcgcaagacg gactacgtcg
     3241 tggccggacc gggcgccggc tcgaagttgg ccaaggccga ggagctcggc gttgccgtgc
     3301 tggacgcgga cggcttccgc gcgctcctgg aacagggaca atgagcgcca tgcagacacc
     3361 gcccgaccag gcagcgcccg atcccgtttc gcaggcagcc gcctaccaac agatgctcct
     3421 cgccgcgctc ggcgacgacg atcccgccga ggtgcaggcg cgcacgcctg ccgacgctcg
     3481 ggcgctgttc gagctcgccg gcccggacct gcgcacgcgg ccggagccgc gcgaatggtc
     3541 cgtgctgctg tgcatcgcgc acatgaccga cggcgaggtc gtgatgtcgg cgcgctaccg
     3601 gtgggtcctc gcccatgacg agccggaatt catcggctac gaccaggatc tctgggccga
     3661 ccggctccat gacggtcagg atccggaggc gctgctcgcg cacttcactg cgctgcgcac
     3721 ggcgaacctg gagctgtggc gccggtcgag cgaggccgac aaggccaggg tcggccagca
     3781 ccgggagcgc ggaccggaga gctacgacct gatgttccgc ctcatgggcg gccatgaccg
     3841 cgtgcatctt gcgcaggcgc ggcgagcgct ggaggccgtc cgcggctgac ctccgcgcgg
     3901 cgacgcgtgc aatgtcacat tcggcccgca cgtagattcg actgcatatg gacgcccttg
     3961 cccgtggagc agcacgcccg cacgccctcg cggcgttcgt ggcgattggc ctgatcgcgt
     4021 tcaccgcgct cggcctctcg acgccccagc ggttcgcagc ggccgagggc aggacgctcc
     4081 acgtgccggc cgagcacgcg accctggagg cggcgatcgc ggcgtcgacc gccggtgacg
     4141 tcatcctgct cgcggcgggg acgtaccccg gcaacgtcgt ggtccccgaa tccaggcccg
     4201 gaatcacgat ccacggcgag gaccgcaact cggtcgtctt cgatggcgcc gacacccggg
     4261 cgaacgcgat tgaggtcgag gccgacgggg tcacgctcga gaacatgtcc gcccacaact
     4321 tcacggacaa cggcttctac tgggacggcg tcgacggctt cgccggccgc tacctcacgg
     4381 tctggaacgt cgggctgtat gcgatctacg cgatcgagag ccgccacggg atcatcgagc
     4441 acagctacgc gtcgggtgcc gccgatgccg cgttctacat cggcgagtgc aacccgtgcg
     4501 acacgacgct gcgcgatctc atcgcggtgc tgtcggcggt cggctactcg ggcacgaacg
     4561 ccggcgggaa cctcgtgatc gaggactcac ggttcgagaa caacggcatc ggcatcctgc
     4621 cgaactcgtt cgaggttggc ctcgagccgc cgccgcagcg cgacgcgatc gtccggcaca
     4681 acgtggtgat cgggagcggc agcgtgccaa cgccccgtgc aacgcccctc ggcgggtacc
     4741 acggcatcgg gatcggggtc gcaggcggcg tcggcaacgt catcgagggc aacgaggtca
     4801 cgggcagcgc gcgctacggg atcgccgtgt tcacggcggt cgatcgcgaa acgccgtggg
     4861 tatccgccga caactcgatc aagagcaacc aggtttcgag cagcggcctg gccgacctcg
     4921 ccctcgctgc cgggagcggc gccaacaact gctttaccgg caatacagcg gccgtgacgc
     4981 tgcccgtgga ccttgcggct tcctgctccg tgctgggcgc gggcgacccg acggtcgccg
     5041 ggaacctcaa cgtggcgccg ccacgacttc tggagggcct gcccgaagcc ccgagttacg
     5101 cgacgatgcc cgcgccgccc gtgcagcaga ccatggcggt cggcgacgcg acgatgaacg
     5161 acgtcgtgct cttcatcgcc gccgtggtcg ccgccggcct ggccctggtc acgctcctcg
     5221 cgttcggttt catgaggctg cgtcgccggc gaaccctgac gggctgatga cccgaagggt
     5281 cagcccatcg cgtggttgaa ctggccccag atccagctcg ggtgccagtg caacggctcc
     5341 ccgcggatca ttcgcggcgc cgaccagcgt tgctcgaacc cgagccccac gagcttcgcc
     5401 gcgccggcgg cgttgcccgc aaggatgccg acgcgcaccc gtccggtcgg gccggacgtc
     5461 tcgcgtcgcg ccgtcacgat cgccgtcgcg gcatccggcg agcgagcaac cgtcgcaccc
     5521 ccgccccacg gcgctcggat cacgaagccc ccgagcgatc catcgggcat gacggccacc
     5581 ttcgcggtct cgggcgctgc gaatcgttcc agggcatggc ggcggtcctc gcccgtggcg
     5641 gcgcgatcga gggcgtgcag gaagtcgaga tcggacggat tgaatgggcg cacgacggga
     5701 ccctcggcgg ccgcggtcgt ggagcccgcg gatcccgtcg gcaggccggg cgcctccagg
     5761 attcggtacc gcgtctggac gcggaagtcc atcttctcgt acaggcggcg accatcctgc
     5821 gtcgccacga gcaccagtgt ccggcagccg gcctcctcca gctggtcgat gatcgcctgc
     5881 gtgagcgcgc ggccgagtcc ctggccgcgc cactcgggct cgaggaagat ggtgccgatc
     5941 cagccaacag cgccgttcgc ggtgcccacg ccggtaccga cgatcacgcc atcggcctcg
     6001 gcgacgatcg gcctacatgc ggcctgggtc gctgcgaacg acagccagtc gcggcgcacg
     6061 ccccagtcgt tgcgcaggat catctcggtc gccgcttcga cgtcggcggg gagcatccgg
     6121 cggagggtgg gacggagggt cgccatcggt gttcagtatc atccgccggt ggccggactc
     6181 actcgaacgg acgtcgagca cgtcgcgttc ctcgctcgcc tgcgcctgac cgacgaggag
     6241 ctcgtgcggc tcgaaggcca gctcaaccac atcctcgacc agtacgcgaa gctgtccgag
     6301 ctcgacactt cggcgatccc gccgaccgcc cagacgatcg agctcgagaa catcctgcgc
     6361 gacgatgtcg ctgggccgtc gctcgccgtc gaggacgtgt tgcggaatgc aagggtcacc
     6421 gagggcgact tcatcgtcgt gccgccgatc ctcggcggcg acgcgtgagc cggcccgcga
     6481 tgttggcgta tcgccgcgtt gtcgtctgcg cgcgctcgct cgcgcacatt gcagtgcgct
     6541 cgcgaacgtg ctcgactccg ccttgcgctc caccaccctc gcgggccggc tcgtgatcga
     6601 cctcacgcga gccctcgggc accagctcgc cgcccagctc cgggccggag aggtgtcgtc
     6661 gcgcgagctg accctcgcgc acctcgacgc cgccgagcgc cagaaccacg cgctgaacgc
     6721 ctggctcgtg atcgagcgcg acaaggcgct ggccgaggca gatgccgccg acgcccgcct
     6781 cgccgcagcc cgcgcgggag gaccggccgc agtggacgcg ctgcatccgc tcctgggcct
     6841 gccggtggcc gtgaaggatc tcgtctccgt ggccggtggc cagtgcaccg caggttcgag
     6901 gatcctcgag ggctacgtcg cgccatacga cgcgcacgt
//
LOCUS       DATBSW010000018         5993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_988580, whole genome shotgun sequence.
ACCESSION   DATBSW010000018 DATBSW010000000
VERSION     DATBSW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988580"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /gene="recJ"
                     /locus_tag="VIF63_00570"
     CDS             complement(<1..1650)
                     /gene="recJ"
                     /locus_tag="VIF63_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY8237904.1"
                     /translation="MLSPRSRWIFPNPVTIPPTLLDVGLEIGLGPRVVALLAARGVDT
                     EDDLRRFLGDPAGSLHDPRRLPDADVFEARVQAARDRGERVLVFGDFDADGITGLTIL
                     TLALRALAIDAIPYVPSRLEEGHGLSLAAVEAAKEAGATVIVTVDTGSSSVDEVAAAQ
                     AAGIDVLITDHHRVPAVLPPAHAIVNPHRADASYPDRRLAGSGVAFTLARLLIGDSAL
                     AFADLAAIGTVADLAPVLGENRAIVQLGLERIRSGPRPGLVALLAAARIEASAVDLES
                     LSFGIAPRLNAAGRVGEAFDAARLLLSESPEAAAELAATLEAANLTRRDLMKTAIAEA
                     RTSPDAVADAAATVVRGPWPVGIVGLVASRLADERRRPAIVGADLGSVVRASCRSANG
                     LHLADTLAECSDLLIRHGGHAGAAGFEIATERWDEFRERFLALAAAAGPPDPVPTLAL
                     DLAIRGRDVDYALHRDLQKLAPCGMGNPEPLVAILGMTVTRVRETGDGGHTTLVLKRR
                     LDVLDGIAFGWPELAREVAEGDVIDIVAQLRSRRFGGIESLQ"
     gene            complement(1707..2828)
                     /gene="secF"
                     /locus_tag="VIF63_00575"
     CDS             complement(1707..2828)
                     /gene="secF"
                     /locus_tag="VIF63_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8237905.1"
                     /translation="MFDIVGKRNWFFLLSLLVTIPGVIFILLTPLTNGQAGLKFSIDY
                     TGGTEWSIRFADPSVTPDAVRLELEALGQDDAQVTKTGRGFLDIRMSKLDLREEPTPT
                     ATPTAAPTVAPSVVGPVASMPPTAAPTATPVPSETPAPSATPIPGSANAVPTEGDIGA
                     IATALQAKFGPIAEQAKLSSIGAVVSADLISQAFLLIIIGSLGILGWITYRFRDFKFG
                     VTALVALLHDVIVVVGIFALLGTLFGVKIDGLFVTAMLTVIGFSVHDTIVVFDRIREN
                     RARHAGEPFAEVVNHSILQTFGRSITTSFTVVLTLTTLLLFGGSATQEFVLALLIGIV
                     SGTYSSIFNAAPLLVVWQEWEDRRKGRLAPPGKARRVPA"
     gene            complement(2821..4206)
                     /gene="secD"
                     /locus_tag="VIF63_00580"
     CDS             complement(2821..4206)
                     /gene="secD"
                     /locus_tag="VIF63_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY8237906.1"
                     /translation="MRRTGPILIAVIGLLALAIDFWPNLTLPALTQNEGPRKIETKLG
                     LDLQGGLKVEYRVNPAGGKTPSQADVDVVKQIIEARVNATGVSEPVVVTSGTDRIIVE
                     VPAITDPAAIRRLVGQTGRLDFIPLPREKYGTEGAAGPQVATQGEPLPTTETPLFSGD
                     QVTSANVNTDQQGKRVVSFTLGETGKELFATYTSTHVREFFAIVLDGRVISAPFIESA
                     ILGGSGQIGGPGLGGFARAEAEELVNVLRFGSLPFPVEELASDTIAPTLGQEFLNSSL
                     LAGAIAIMLVVFFMLVHYRLPGLIASFALLYYSVLVLALFRLIPVTLTLAGIAGFVLS
                     IGMAVDANILIFERTKEELRSGKSLAQAIEAGFARAWNSILDSNVSSLITAMILYLFG
                     SSTIQGFALVLIIGVLASMFTAIVVTRSVLRVVVAREWARKAWLYGVLDSEFTARPTT
                     RSGLRREPRSV"
     gene            complement(4225..>5993)
                     /gene="uvrC"
                     /locus_tag="VIF63_00585"
     CDS             complement(4225..>5993)
                     /gene="uvrC"
                     /locus_tag="VIF63_00585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8237907.1"
                     /translation="DSVGEALLLEGNLVKRFQPAFNVRLKDDKSYPYIKITLNDDFPR
                     IERTRKLLNDGSRYFGPYASASSVDESMNLVRRLFPFRTCTIDIKDGVRALQRPCLLY
                     HIKRCQGPCIEAISKDEYRADIEQVELFLEGRQESLVKALRDDMKRASDRLDYEHAGI
                     TRDKIKAIERTMESQKMAAFARTDLDLLGLARRDNQAAVQLFVIRGGKALGRDVFMLD
                     TPRGATDADVVSGFLLQYYARATSVPPEIAIPLVLPEHADLERFLADRRAGAGAADAE
                     NGSGPERSLNRTENLTVPRPDAVRVTRTRTRAAGVAAPVRLVVPQRGERRKLMVLATR
                     NAEEMLAREQARWLADQGKTLGALEELADALGLPGLPARIECYDVSNFQGSETVASMV
                     VFEDGKPRTGEYRRFRVRTVEGPNDVASHQEVLRRRFRGARPGDEGSAEERRWAMPDL
                     VLIDGGKPQVGAAKTVLDELGLHDTPLAGLAKEREELVLPDRAEPVLLPTTSQALYLV
                     QRIRDEAHRFAITYHRDLRRKAAVKSKFDDLPGVGPRRRGALLRVFGSMKRVREAPVE
                     QVAAVPGIGPALAARIKAALDA"
BASE COUNT          898 a         2009 c         2214 g          872 t
ORIGIN      
        1 ctggagcgac tcgatgccgc cgaagcggcg gctccggagc tgggcgacga tgtcgatcac
       61 gtcgccctcg gcgacctcgc gggcgagctc cggccagccg aaggcgatcc cgtcgagcac
      121 gtcgaggcgg cgcttcagca ccagcgtcgt gtgcccgccg tcgccggtct cgcggacgcg
      181 ggtcacggtc atgcccagga tcgcgacgag cggctcggga ttgcccatgc cgcatggggc
      241 gagcttctgc aggtcccgat gcagggcata gtcgacgtcg cgtccccgga tcgcgaggtc
      301 gagcgcgagg gtcggcacgg gatccggcgg gccggccgcc gcggcgaggg ccaggaaccg
      361 ctcgcggaac tcatcccacc tttcggtcgc gatctcgaag ccggctgcgc cggcgtggcc
      421 gccgtggcgg atcaggaggt ccgagcactc ggcgagggtg tcggcaaggt gcagcccgtt
      481 cgcgctccgg cacgacgccc tgaccacgga cccgaggtcc gcgcccacga tcgccggccg
      541 gcgccgctcg tcggcgaggc gcgatgccac gaggcccacg atcccgaccg gccagggccc
      601 gcggacgacc gtcgccgccg cgtccgcgac cgcgtcgggg gacgtgcgcg cctcggcgat
      661 cgcggttttc atcaggtcgc gccgggtcag gttggccgcc tcgagcgtgg cggccagctc
      721 ggcggccgcc tcggggctct cgctgagcag cagccgggca gcgtcgaacg cctcccccac
      781 cctgcccgcg gcattcaggc gcggcgcgat gccgaacgac aggctctcca ggtcgaccgc
      841 ggacgcctcg atccgggcgg cggcgagcag ggccacgagc ccgggccggg ggccgctccg
      901 gatccgctcc agcccgagct ggacgatcgc ccggttctcg ccgagcacgg gggcgaggtc
      961 tgccacggtg ccgatcgcgg cgaggtccgc gaaggccagc gccgagtcgc cgatcagcag
     1021 tcgcgccagc gtgaacgcga cgccgctgcc cgcgagtcgc ctgtccggat aggacgcgtc
     1081 ggcgcggtgc gggttgacga ttgcgtgagc gggcgggagc accgcgggca cgcggtggtg
     1141 gtcggtgatg aggacgtcaa tgcccgccgc ctgcgcggcc gcgacctcat cgacgctcga
     1201 gctgcccgtg tcgacggtca cgatgacggt ggcgcctgct tcctttgccg cctccacggc
     1261 ggcgagcgac aggccgtggc cctcttcgag gcgcgacggg acgtacggga tcgcatcgat
     1321 ggcgagcgcg cgcagggcga gcgtcaggat cgtgaggccg gtgatgccat ccgcgtcgaa
     1381 gtcgccgaac accaggacgc gctcgccgcg gtcgcgcgcc gcttgcactc gcgcttcgaa
     1441 gacgtccgcg tcggggagtc gccgcgggtc atgcaacgag cccgccgggt cgccgaggaa
     1501 cctccggagg tcgtcttcgg tgtcgacgcc gcgggccgcg agcagggcca cgacacgcgg
     1561 cccgagcccg atctccagcc cgacatcgag cagggtcgga ggaatggtga cggggttcgg
     1621 gaagatccag cgactgcgcg gggagagcac gtcgtcagga tgtcacgcgg cgctgcaccg
     1681 cggctctacc gccggcccga agccggtcag gcagggacgc gtcgagcctt gccgggaggc
     1741 gcgaggcggc ccttccgccg gtcctcccac tcctgccaga cgacgaggag cggcgccgcg
     1801 ttgaagatcg acgagtacgt cccggacacg atgccgatca gcagcgcgag gacgaactcc
     1861 tgggtcgccg agcccccgaa aaggagcagc gtcgtgagcg tgagcacgac cgtgaagctc
     1921 gtcgtgatcg accggccgaa tgtctgcagg atcgagtggt tgaccacctc cgcgaacggc
     1981 tcgccggcgt ggcgggcccg gttctcgcga attcggtcga agacgacgat cgtgtcgtgg
     2041 acgctgaagc cgatgacggt gagcatggcc gtcacgaaga ggccgtcgat cttgaccccg
     2101 aacagcgttc cgaggagcgc gaagatcccg acgacgacga tgacgtcgtg gagcagcgcg
     2161 acgagcgcgg tcacgccgaa cttgaagtcc cggaaccggt aggtgatcca gccgaggatg
     2221 ccgagcgagc cgatgatgat cagcaggaac gcctgcgaga tcaggtcggc gctcacgacg
     2281 gcgccgatcg agctgagctt cgcctgctcg gcgatcgggc cgaacttcgc ctggagcgcg
     2341 gttgcgatcg cgccgatatc gccctcggtc ggcacggcgt tggccgagcc ggggatcggt
     2401 gtcgcactcg gcgccggtgt ttccgagggc accggtgtcg cggtcggtgc ggcggtgggc
     2461 ggcatcgagg caaccgggcc gacgacgctc ggcgccacgg tgggggccgc ggtcggcgtg
     2521 gcggtcgggg tcggctcctc acgcaggtcg agcttgctca tgcgaatgtc caggaacccg
     2581 cggccggtct tggtgacctg cgcgtcgtcc tggcccaggg cctcgagctc gaggcgcacg
     2641 gcgtccggcg tcaccgacgg atcggcgaag cggatcgacc attccgtgcc gccggtgtag
     2701 tcgatcgaga acttcaaccc cgcctggccg ttggtcagtg gcgtcaggag gatgaagatg
     2761 acgcccggga tcgtgaccag gagcgagagg aggaagaacc agttccgctt gccaacgatg
     2821 tcaaacactg cggggctcgc gccgcaggcc gctccgggtc gtcggccggg cggtgaactc
     2881 ggagtccagc acgccgtaca gccaggcctt gcgggcccac tcgcgggcga cgacgacgcg
     2941 caggacggag cgggtcacga cgatcgcggt gaacatcgag gcgagcacgc cgatgatcag
     3001 gacgagggcg aagccctgga tcgtcgatga cccgaacagg tacaggatca tcgcggtgat
     3061 caggctcgac acgttggagt cgaggatgga gttccaggcc cgggcgaagc cggcctcgat
     3121 cgcctgcgcg agggacttgc ccgaacgcaa ctcctccttc gtccgctcga agatcaggat
     3181 gttggcgtcg accgccatac cgatcgagag cacgaagccc gcgatgccgg cgagcgtcag
     3241 ggtcacgggg atcagccgga acagggccag cacgagcacc gagtagtaga gcagcgcaaa
     3301 gctggcgatc aggcccggca gccggtagtg cacgagcatg aagaagacca cgagcatgat
     3361 tgcgatggcc ccggcgagca ggctgctgtt gaggaactcc tggccgagcg tcggggcgat
     3421 ggtgtcgctg gccagctcct ccaccgggaa cggcagcgag ccgaagcgca ggacgttgac
     3481 aagctcctcg gcctcggccc gcgcgaagcc gccgagcccg gggccgccga tctggcccga
     3541 gccgccgagg atggcgctct cgatgaacgg cgccgagatg acccggccgt cgagcacgat
     3601 cgcgaagaac tcgcggacgt gtgtcgacgt gtacgtggca aacagctctt tccctgtttc
     3661 accaagggtg aacgagacga cgcgcttgcc ctgctgatcg gtgttgacgt tcgcgctcgt
     3721 gacctggtcg cccgagaaca gcggcgtctc cgtggtcggc agcggttcgc cctgggtggc
     3781 cacctgcggc ccggccgcgc cctcggtgcc atatttctcg cgcggcagcg ggatgaagtc
     3841 gagccgtccg gtctgcccga cgagtcgccg aatcgcggcg gggtcggtga tcgcaggcac
     3901 ctcgacgatg atccggtccg tgcccgatgt cacgacgacc ggctcggaga caccagtcgc
     3961 gttcacacgc gcctcgatga tctgcttcac gacgtcgacg tcggcctggg acggggtctt
     4021 gccgcccgcc gggttcacgc ggtactcgac cttcaggccg ccctggaggt caaggccgag
     4081 cttcgtctcg atcttgcgtg ggccttcgtt ctgggtgagc gcgggcagcg tcaggttcgg
     4141 ccagaagtcg atggccaggg caagcagccc gatgacggcg atgaggatcg gtccggttct
     4201 acgcacgagg gcggatgata caggtcaggc gtcgagggcg gctttgatcc tcgcggcaag
     4261 ggccggcccg ataccgggta ctgcggccac ctgctcgacc ggggcttcgc gaacgcgttt
     4321 catcgagccg aagacgcgca gcagggcgcc gcggcggcgc gggccgacgc cgggcaggtc
     4381 gtcgaacttg gacttcaccg ccgccttgcg gcgcaggtcg cggtggtacg tgatggcgaa
     4441 ccggtgcgcc tcgtcgcgaa tccgctgcac gagatagagc gcctgcgagg tcgtcgggag
     4501 caggacgggc tccgcgcgat cagggaggac cagctcctca cgctccttgg cgagcccggc
     4561 gagcggcgtg tcgtgcagcc cgagctcgtc gaggaccgtc ttcgcggcgc cgacctgggg
     4621 cttgccaccg tcgatgagga cgaggtcggg catcgcccag cggcgctcct cggcgctgcc
     4681 ctcgtcgccg ggtcgggcgc cccggaatcg gcgccgcagg acctcctggt gggaggcgac
     4741 gtcgttcggg ccctcgacgg ttcgaacccg gaagcgccgg tactcccccg tccgcggctt
     4801 gccatcctcg aacacgacca tgctcgcgac ggtctcgctg ccctggaagt tcgagacgtc
     4861 gtagcactcg atccgcgccg gcaagccggg caggccgagc gcatcggcga gctcctcgag
     4921 ggcgccgagg gtcttgccct gatcggcgag ccaccgtgcc tgctcgcgcg cgagcatctc
     4981 ctcggcgttg cgcgtcgcga ggaccatgag cttgcgccgc tcgcctcgct ggggcacgac
     5041 gaggcgcacg ggcgcggcca ctcccgccgc gcgggtcctc gtgcgcgtga cgcggactgc
     5101 gtcggggcgc gggacggtga ggttctcggt tcggttgagg gagcgctcgg ggcctgaccc
     5161 gttctcggcg tcggcagcgc cggcgccggc ccgccgatct gccaggaacc gctcgaggtc
     5221 ggcgtgctcc gggagcacga gcgggatcgc gatctccggc gggacgctcg tcgcgcgggc
     5281 gtagtactgg agcaggaagc ccgagacgac gtcggcgtcg gtcgcgccgc gcggggtgtc
     5341 gagcatgaag acgtcgcgcc cgagcgcctt gccgccgcgg atcacgaaca gctggaccgc
     5401 ggcctggttg tcgcggcggg ccagacccag caggtccaga tctgtccggg cgaatgcggc
     5461 catcttctgg ctctccatcg tccgctcgat cgccttgatc ttgtcgcgcg tgatcccggc
     5521 gtgctcgtag tccagccggt cggatgcccg cttcatgtcg tcgcgcagcg ctttcaccag
     5581 ggattcctgg cggccctcga ggaacagctc gacctgctcg atgtcggccc ggtactcgtc
     5641 cttcgagatc gcctcgatgc acgggccctg gcagcgcttg atgtggtaca gcaggcacgg
     5701 ccgctggagc gcgcgcacgc cgtccttgat atcgatcgtg caggtccgga acgggaagag
     5761 gcgtcggacg aggttcattg actcgtcgac gctggacgcg gacgcatacg gcccgaagta
     5821 acggctgccg tcgttgagca gcttgcgcgt ccgctcgatg cgcgggaagt cgtcgttgag
     5881 cgtgatcttg atgtacgggt agctcttgtc gtccttcagg cgcacgttga acgcgggctg
     5941 gaagcgcttc acgaggttgc cctcgagcag gagcgcctcg ccgaccgagt ccg
//
LOCUS       DATBSW010000019         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1164992, whole genome shotgun sequence.
ACCESSION   DATBSW010000019 DATBSW010000000
VERSION     DATBSW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164992"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /gene="glmS"
                     /locus_tag="VIF63_00590"
     CDS             <1..1866
                     /gene="glmS"
                     /locus_tag="VIF63_00590"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY8237908.1"
                     /translation="AHRFARGVSFWMCGIVGYTGPRDAGPILMEGLRRLEYRGYDSAG
                     IALVDGQGDLFMEKRAGKLGNLRTAIAERTPHAALGLAHTRWATHGRPNDINAHPQVD
                     CTGRITVIHNGIIENFQELRDGLEARGHTLVSETDTEAIAHLIEEAYDGDIAEASRAA
                     MRQLTGAYALAIMHRDENDRLVGARKDVPLVVGIGEGEAFLASDVAAILGDTRRVIFL
                     EEGDVADLTPNRVIVTDVEGRPVERVVETIEWTVEAAEKGGFDTFMLKEIHEQPEALR
                     QSLTGRISLAGRIQSPELEPVFEAVRRATRVELVACGTASYAAMVGARAIQELTGLPA
                     RVTVGSEFRYDPPPLDEQTLVIAVTQSGETADTIAPTRLARERGCPIIAVTNTLGSAI
                     TREADAVLFLQAGPEMAVAASKTFVTQVTTLVVLAAGIAKLRGTLTDAAEAELVAGLR
                     ALPAAAQRAVELNEPNSERLARRYVNSRGFMFVGRGATYPAALEGALKLKEISYVHAE
                     GYPAGELKHGPISLLDAECPLVAVATRSSTYDKLISNVMEGRARDAKVIAVATEGDEG
                     IGRIADDVCWVPDTHEMLSPVLAIIPLQLFAYHVALARGTDVDQPRNLAKSVTVE"
     gene            1880..2416
                     /gene="acpS"
                     /locus_tag="VIF63_00595"
     CDS             1880..2416
                     /gene="acpS"
                     /locus_tag="VIF63_00595"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY8237909.1"
                     /translation="MVAPEDAVAAGITPPPGTTELGIDIIKVSRIRDSLAKFGRRFPR
                     RVLTENEDRYVRDRPETFAGRWAAKEAVSKVLGLGVRGIGWRDIEIERLPTGQPSVRL
                     HGRAAARAEQLGMGRIAVSITHEAEYAVAIAFGVRTAGGKYVFPPDIEARLDDRERRI
                     LARMERMRAMAESTPRDG"
     gene            2409..3458
                     /locus_tag="VIF63_00600"
     CDS             2409..3458
                     /locus_tag="VIF63_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY8237910.1"
                     /translation="MAERRPLGFGRSETPVDPASAPASAVPTMDDRAAAELLPERPKR
                     GHKGTFGKVLVIAGSLDYAGAALLVCRAAGRAGAGLVTLAVPESLQPLFAAKVVEATT
                     MALPEDDVEEVDPEPAIARILDHEHDAIVVGPGLRPGLATAELVRMLLAATDEQSPPL
                     VLDAEALRSMATLGEWWTGTSRPCVLTPHPGEFERLRAGSGIEPSADVDLSADDEARR
                     AAALEAATTWRQVIVLKGAKTVIAEPDGTVTVAPFENPALATGGTGDVLAGIIGALLA
                     QGLAPGPAARLGVYLHGTAGDVVRERLGDAGLLASDLPDVVPGVRKRLAAIAERASGA
                     KRLGFAVRDGDGAGA"
     gene            3515..4750
                     /gene="alr"
                     /locus_tag="VIF63_00605"
     CDS             3515..4750
                     /gene="alr"
                     /locus_tag="VIF63_00605"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8237911.1"
                     /translation="MPRTAWAEIDLAAIAVNLGVLRRLAGDGTKVNPVVKADAYGHGA
                     VPVARALEAAGADGFCVATFDEALALRADGIALPILVLYPIPTALATAAARERIAVTA
                     GDRTLLDALLVALAAEPPAEALRIQLEVETGLGRGGFALAELGAAARDVTLAADGRLA
                     RFAGMWTHFQAPEDLDKTSSQVAVFEQAVAMLEAEGVAVPVRHVAASGGLLLDDVVSL
                     EGVRPGLTTYGLLPDELLDPATPNPVPGAADLRPALSLHARPVRVIDVPAGSGISYGP
                     TFTTSRPSRIATLPLGYGDGWPRSLSNRASALVRGQRVPLVGNVAMDAVMADVTDVLG
                     EPVGVGDEFVLIGEQGDQRITAAEVAAARGTNSWEVVTNLSARLPRVYHAASVPLETR
                     TLTGEAVGRDAGARRSKRP"
     gene            4747..5037
                     /locus_tag="VIF63_00610"
     CDS             4747..5037
                     /locus_tag="VIF63_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237912.1"
                     /translation="MSELGGLGDEQRDELVEEVAREIQLRGLTTSAVHFLQASRPYRP
                     LGSNAMLFFDPVLHRLFGGDMPEASALLADEDGIEQLIERLEDLSEETGWDA"
BASE COUNT          728 a         1871 c         1873 g          712 t
ORIGIN      
        1 gctcaccgct tcgctagggg agtctcgttc tggatgtgcg gaatcgtcgg ctacacgggg
       61 cctcgcgacg ccggcccgat cctcatggaa ggcctgcgcc gcctcgaata ccgcggctat
      121 gactccgccg gcatcgccct cgtcgacggc cagggcgacc tgttcatgga gaagcgcgcc
      181 ggcaagctcg gcaacctgcg caccgccatc gccgagcgca ccccgcatgc cgccctcggc
      241 ctggcccaca cgcgctgggc gacgcacggt cgcccgaacg acatcaacgc ccatccccag
      301 gtggactgca ccggccggat caccgtcatc cacaacggga tcatcgagaa cttccaggag
      361 ctgcgcgacg gcctggaggc ccgcggccac acgctcgtct ccgagaccga caccgaggcg
      421 atcgcccacc tgatcgagga ggcctacgat ggcgacatcg ccgaggcctc gcgggcggcc
      481 atgcgccagc tcaccggcgc gtatgccctc gcgatcatgc atcgcgacga gaacgaccgg
      541 ctcgtgggcg cgcgcaagga cgtgccgctg gtcgtcggca tcggcgaggg cgaggcattc
      601 ctcgcctcgg acgtggccgc gatcctcggc gacacgcgcc gggtgatctt cctcgaggag
      661 ggcgacgtcg ccgacctgac cccgaatcgg gtgatcgtca cggacgtcga ggggcggccg
      721 gtggagcgtg tcgtcgagac gatcgaatgg accgtggagg ccgccgagaa gggcggcttc
      781 gacacgttca tgctcaagga gatccacgag cagccggagg cactgcgcca gtcgctgacg
      841 ggccggatct cgctcgcggg ccggatccag tcacccgagc tggagcccgt gttcgaggcc
      901 gtccggcgcg ccacgcgcgt cgagctcgtc gcctgcggca cggcctccta tgcagcgatg
      961 gtcggggcac gggcgatcca ggagctgacc ggcctgccgg cccgagtgac ggtgggctcg
     1021 gagttccggt acgacccgcc gcccctcgac gagcagacgc tcgtgatcgc cgtgacccag
     1081 tccggcgaga cggccgacac gatcgccccg acccggctcg cccgggagcg cggctgcccg
     1141 atcatcgccg tgacgaacac gctcggctcg gcgatcacgc gcgaggcgga cgcggtgctg
     1201 ttcctgcagg caggcccgga gatggccgtg gcggcgagca agacgttcgt cacccaggtg
     1261 acgacgctgg tcgtgctcgc ggcgggcatc gccaagctgc gcggcacgct caccgacgcg
     1321 gccgaggcgg agctcgttgc ggggttgcgc gcgctgccgg cggccgctca gcgcgcggtc
     1381 gagctcaacg agccgaactc cgaacgcctc gcgcggcgct acgtcaactc gcgcggcttc
     1441 atgttcgttg ggcgcggggc aacgtatccc gccgctctgg agggcgccct caagctcaag
     1501 gagatcagct acgtccatgc cgagggctac ccggccggtg agctgaagca cggcccgatc
     1561 tcgctcctcg atgcggagtg tccgctcgtc gcggtcgcaa cccgctcgtc gacgtacgac
     1621 aagctcatca gcaacgtgat ggagggccgg gcgcgcgacg ccaaggtcat cgcggtcgcg
     1681 acggaaggcg acgagggcat cggccggatc gccgacgacg tgtgctgggt ccccgacacg
     1741 cacgagatgc tgagcccggt gctcgcgatc atcccgctcc agctcttcgc ctaccacgtg
     1801 gcgctcgcgc gaggcacgga cgtcgaccag ccgcgcaacc tggcgaagtc ggtcacggtc
     1861 gagtagggcg gtcctccgga tggtcgcgcc cgaggacgcc gtcgctgccg gaatcacgcc
     1921 cccaccgggc acgacggagc tgggcatcga catcatcaag gtgtcccgga tccgggattc
     1981 gctggcgaag ttcggacggc gctttccgcg ccgggtgctg accgagaacg aggaccgcta
     2041 cgtccgcgat cggcccgaga cattcgccgg ccgctgggcc gccaaggaag ccgtgagcaa
     2101 ggtgctcggc ctgggcgtcc gcgggatcgg ctggcgcgac atcgagatcg agcgactgcc
     2161 gaccggccag ccctcggtgc ggttgcacgg ccgggcggcg gcccgggcgg agcagctggg
     2221 catgggccgg atcgcggtgt cgatcacgca tgaggccgag tacgcggtcg ccatcgcgtt
     2281 cggcgtgcgc accgctggtg gcaagtacgt gttcccgccg gacatcgagg cccggctcga
     2341 cgaccgcgag cgccggatcc tggcgcgcat ggagcggatg cgggcgatgg ccgaatcgac
     2401 gccgcgcgat ggctgagcgg cgacccctgg gcttcgggcg cagcgagacc cccgtggatc
     2461 cggcctcggc gccggcctcc gccgtgccga ccatggatga ccgcgccgcg gcggagctcc
     2521 tgccggagcg acccaagcga ggccacaagg ggacgttcgg caaggtgctc gtgatcgccg
     2581 gatcgctcga ctacgcaggc gccgcgctgc tcgtgtgccg cgcggcggga agggccggcg
     2641 ccgggctcgt caccttggcg gtcccggagt cactgcagcc attgttcgcc gcgaaggttg
     2701 tcgaggcgac gaccatggcc ctgcccgagg acgatgtcga ggaggtcgat cccgagccgg
     2761 cgatcgcccg gatcctcgat cacgagcacg acgcgatcgt cgtcgggccg gggctgcgtc
     2821 cgggcctcgc gacggccgag ctggttcgca tgctccttgc cgcgaccgac gagcagtcgc
     2881 cgcccctcgt cctcgatgcc gaggcgctgc ggtcgatggc cacgctcggc gagtggtgga
     2941 cgggcacgag ccggccgtgc gtcctcaccc cgcaccccgg tgagttcgag cggctccggg
     3001 ccggctccgg catcgagccg tcggccgacg tcgacctttc ggccgatgac gaggcgcgcc
     3061 gtgcagcggc gctcgaggcc gcgacgacat ggcgccaggt catcgtcctc aagggcgcga
     3121 agaccgtcat cgccgagcca gacggaacgg tcaccgtcgc gccattcgag aatccggccc
     3181 ttgccaccgg cggcaccggc gacgtcctcg ccgggatcat cggcgcgctg ctcgcccagg
     3241 gcctggcgcc gggtccggcc gcacgcctcg gcgtgtacct gcacgggacg gcgggtgacg
     3301 tggttcgcga gcgtctcggc gatgccgggc tgctcgcctc ggacctgccc gacgtcgtgc
     3361 ccggggtgcg caagcggctt gccgcaatcg ccgagcgggc gagcggcgcg aagcgcctcg
     3421 gctttgccgt ccgcgacggc gacggagccg gcgcctgagc ggccccgcgc gagcctcgat
     3481 cgaggagcga ctcgaatcgg ccgggctgcc gccgctgcca cgcacggcct gggcggagat
     3541 cgacctcgcc gcgatcgcgg tcaacctggg cgtgctgcgg cggctcgccg gcgatgggac
     3601 caaggtgaat cccgtcgtga aggccgacgc gtacggccat ggcgccgtgc ccgtcgctcg
     3661 ggccctcgaa gccgccgggg ccgacggctt ctgcgtggcg accttcgacg aggccctcgc
     3721 cctgcgggcc gacggcatcg cgctgccgat cctcgtcctg tacccgatcc cgaccgcgct
     3781 cgcgacggcg gcggcgcgcg agcggatcgc ggtcacggcg ggggatcgca ccctcctcga
     3841 tgcgctgctg gtcgcgctcg cggccgaacc acccgccgaa gcgctgcgga tccagctcga
     3901 ggtcgagacc ggcctcgggc gtggcggttt cgccctggcg gagcttgggg cggcagcgcg
     3961 cgacgtgacc ttggccgccg atggccgtct ggcgcgcttc gcgggcatgt ggacgcattt
     4021 ccaggcgccg gaggacctcg acaagacgag cagccaggtc gcggtgttcg agcaggcggt
     4081 ggcgatgctc gaagccgagg gcgtcgccgt tcccgttcga cacgtcgccg cgagcggcgg
     4141 gctgctgctc gacgacgtcg tcagcctcga gggcgtccgc cccggcctca cgacctacgg
     4201 cctcctgccc gacgagctcc tcgatcccgc cacgcccaat cccgtcccgg gtgcggccga
     4261 cctgcgaccc gcgctgtcgc tccacgcccg gcccgtccgc gtcatcgacg tgccggcggg
     4321 ctcgggcatc agctacggcc cgacgttcac gaccagccgt cccagccgga tcgcgacact
     4381 gcccctcggc tacggcgatg gctggccgcg atccctctcg aatcgcgcct ccgcgctggt
     4441 ccgcggccaa cgcgtcccgc tggtcggcaa cgtcgcgatg gacgcggtga tggccgacgt
     4501 caccgatgtg ctcggcgagc ccgtgggtgt cggtgacgag ttcgtgctca tcggggaaca
     4561 gggcgaccag cgaatcacgg ctgccgaggt cgccgcggcg cggggcacga actcctggga
     4621 ggtcgtgacg aacctctccg ctcgcctgcc tcgggtgtac catgcggcct ccgttccgct
     4681 ggagacgcgg accctcaccg gcgaggccgt gggccgtgac gccggggcca ggaggtcgaa
     4741 gcgaccgtga gcgagctcgg cggcctcggc gatgagcagc gcgacgagct ggtcgaggag
     4801 gtcgcgcgcg agatccagct gcgcggcctg acgacgtcgg ccgtgcactt cctgcaggca
     4861 tcccggccat accggccgct cggctccaac gccatgctgt tcttcgatcc cgtgctccac
     4921 cgcctcttcg gcggcgacat gccggaggct tccgcattgc tcgcggacga ggacggtatc
     4981 gagcagctca tcgagcgcct cgaagacctt tccgaagaaa cgggctggga cgcatagcgg
     5041 cacgacgccg ctcgcgaggg ccgacggcgg ttcttgccgg tagcatgacc aagtgacgaa
     5101 cgttccccgc gcgctcctcg cgatcgatac cggagcggcg accacgtcgg ttgccctgct
     5161 cggcaagccg ggcgtgcgct ggcg
//
LOCUS       DATBSW010000020         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_741755, whole genome shotgun sequence.
ACCESSION   DATBSW010000020 DATBSW010000000
VERSION     DATBSW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741755"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="ftsZ"
                     /locus_tag="VIF63_00615"
     CDS             complement(<1..875)
                     /gene="ftsZ"
                     /locus_tag="VIF63_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8237913.1"
                     /translation="MGLRSDSENFALIRVIGVGGGGSNAVNRMIRAEMMGVEFIALNT
                     DAQALLQSDAPHKLRIGDKITRGLGAGGDPSIGERAAEEDSEKIAQALEGSDMVFITS
                     GLGGGTGSGAAPVVAQIAKELGALTIAVVTKPFTFEGAKRRLTAEKAGESLKANVDTL
                     ITIPNDRLRDVVQKNTSILDAFRVVDDVLRQGVQGISDIITVPGLINLDFADVRAIMK
                     DAGSALMGIGKASGENRAVEAARQAIASPLLEVDIAGAQGILFNISGGPNMSLFEVTE
                     AAEEIRAAADPEANI"
     gene            complement(1050..2279)
                     /gene="ftsA"
                     /locus_tag="VIF63_00620"
     CDS             complement(1050..2279)
                     /gene="ftsA"
                     /locus_tag="VIF63_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8237914.1"
                     /translation="MAEGVLVALDVGTSKVCALIGEIGRDGRLTVIGHGTVPSSGLKR
                     GAVVNIEQTVRSIRDAVERAERLSGWKIDKAFVGVGGAQVESFNSTGQVSVAAHTREV
                     GRDDIRRAVEQARTIPIPSNREVLHVERRGFTVDGQEGVKDPLGMSALRLEVEAHIVT
                     APQTAVQNLMKCVAQAGVKIDELVVNALASSEAVANETERELGVAVADVGAGTIDLAM
                     FNEGSPFRTSVLPVGGNFVTNDIAIGIKTSLPAAEELKVRHGTCDLRNIEEDEEIAVS
                     VLGEEAGRTISRIELCRIIESRMRETFELIGAEIMAGGAGMLPAGLILTGGGAQLAGI
                     AELGREVLSMPVRVVGPSGVGGLTDSILTPAYATSIGLLQWGASMLDDGEPARYESAP
                     AAGVLGRLRSAVKSMLP"
     gene            complement(2288..2611)
                     /locus_tag="VIF63_00625"
     CDS             complement(2288..2611)
                     /locus_tag="VIF63_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEY8237915.1"
                     /translation="MLLPLAGLLVGVLLGLVLNVQVGFDLSRYSAVAILAALDSVLGA
                     VRAELDGVYNNRIFISGFVTNAIVAVILTFIGDRLGLDLYLVALITFGLRIFNNVALI
                     RRHFI"
     gene            complement(2687..>3220)
                     /locus_tag="VIF63_00630"
     CDS             complement(2687..>3220)
                     /locus_tag="VIF63_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237916.1"
                     /translation="VANAEVTVQLPDAVVVVAIEERVPILAWQVGDTRYLADREGTIF
                     ATAPDGDPLPTGVAVIEDRRVGADATLAVGTTLDVVDLDVATRLGSLTPDDVGSAVTR
                     LKVAVTDDDGFIVNAPGSWTAVFGFYSPSTRATDMIPGQVRLLRSLLAGGEGTLTRII
                     LASESGGTYVPKPTRKP"
BASE COUNT          484 a         1141 c         1097 g          498 t
ORIGIN      
        1 atgatgttcg cctcgggatc ggctgcggcg cggatctcct ccgccgcctc ggtgacctcg
       61 aacagcgaca tgttcgggcc gccggaaatg ttgaacagga tgccctgcgc gcccgcgatg
      121 tcgacctcga gcagcgggct cgcgatcgcc tggcgcgccg cctcgacggc ccggttctcg
      181 cccgaggcct tgccgatgcc catgagcgcc gagccggcgt ccttcatgat cgcccgcacg
      241 tcggcgaagt cgaggttgat cagcccgggc acggtgatga tgtcgctgat gccctgcacg
      301 ccctggcgca ggacatcgtc gacgacccgg aacgcgtcga ggatcgacgt gttcttctgg
      361 acgacgtcgc gcagccggtc gttcgggatc gtgatgaggg tgtcgacgtt ggccttgagc
      421 gactcgcccg ccttctcggc cgtcaggcga cgctttgcgc cctcgaaggt gaacggcttg
      481 gtgacgaccg cgatcgtcag cgcgccgagc tccttggcga tctgggccac caccggggcc
      541 gcgcccgagc cggtgccgcc gccgaggccg gaggtgatga acaccatgtc cgagccctcg
      601 agggcctggg cgatcttctc gctgtcctcc tcggccgcgc gctccccgat cgaggggtcg
      661 ccgccggcgc cgaggccgcg cgtgatcttg tcgccgatcc gcagcttgtg cggcgcatcc
      721 gactgcagga gggcctgggc atcggtgttg agggcgatga actccacgcc catcatctcc
      781 gcccgaatca tcctgttgac ggcgttgctg ccgccaccgc cgacgccgat gaccctgatc
      841 agggcgaagt tctccgagtc cgagcgcagg cccatcgcta ctcctccagc ggccggccgt
      901 ggccgcgata aacgggtcgt tgaaagaggc cgggcaccgg cgcgctcgct cgccgcgtct
      961 ctcccgaggt cgtcgccgag tctaccatcg gcgcccctcc gggcgctcac cgaatcacga
     1021 cagaggcgaa agtttccgtc gccttcgggc tacggcagca tgctcttgac ggcgctgcgc
     1081 aggcggccca ggacccccgc cgctggcgcg gactcatagc gcgccggctc gccgtcatcg
     1141 agcatcgacg cgccccactg gagcaggccg atgctggtgg cgtaggcggg cgtgaggatc
     1201 gagtcggtga ggccgccgac gcccgatggc ccgacgacgc ggaccggcat cgaaaggacc
     1261 tctcgcccga gctcggcgat gcccgcgagc tgcgcgccgc cgccggtcag gatcaggccc
     1321 gcgggcagca tgcccgcgcc accggccatg atctcggcgc cgatgagctc gaacgtctcg
     1381 cgcatgcgcg attcgatgat ccggcagagc tcgatccggc tgatcgtgcg gccagcctcc
     1441 tcgcccagca cggagacggc gatctcctcg tcctcctcga tgttgcgcag gtcacacgtg
     1501 ccgtggcgga ccttgagctc ctccgcggcg ggcagcgagg tcttgatgcc gatcgcgatg
     1561 tcgttcgtga cgaagttgcc accgaccggc agcacgctcg ttcggaaggg cgagccctcg
     1621 ttgaacatgg ccaggtcgat cgtgcccgcg cccacgtccg cgacggcgac gccgagctcg
     1681 cgctccgtct cgttggcgac cgcctccgac gaggccagcg cattgacgac cagctcgtcg
     1741 atcttcacgc ccgcctgggc gacgcatttc atgaggttct gcacggcggt ctggggcgcg
     1801 gtgacgatgt gcgcctcgac ctcgagccgc agcgcgctca tcccgagcgg gtccttgacg
     1861 ccctcctggc cgtcgacggt gaagccgcga cgctcgacgt ggaggacctc gcggttcgag
     1921 gggatcggga tcgtccgggc ctgctcgacg gcacgccgga tgtcgtcccg gccgacctcg
     1981 cgggtatgtg ctgcgaccga cacctggccg gtcgagttga agctctcgac ctgcgcgccg
     2041 ccgacgccga cgaaggcctt gtcgatcttc cagccggaca ggcgctcggc ccgctcgacc
     2101 gcgtcccgga tcgagcgcac ggtctgctcg atgttcacga ccgcgccgcg cttcagcccc
     2161 gacgagggca ccgtgccatg gccgatcacg gtgagccggc cgtcgcggcc gatctcgccg
     2221 atcagcgcgc agaccttgct cgttccgacg tcgagtgcga ccaggacccc ttctgccacg
     2281 ccctacctca gatgaagtgc cgccggatca gggcgacgtt gttgaagatt cgcaatccga
     2341 acgtgatcag cgccaccagg tacagatcca ggccgagtcg atccccgatg aaggtcagga
     2401 tcacggcgac gatcgcgttg gtgacgaagc ccgagatgaa aatcctgttg ttgtagacgc
     2461 catcgagctc ggcccgcact gctccgagca ccgagtcgag cgcggcgaga atcgccacgg
     2521 ccgagtagcg gctgagatcg aagccgactt gcacattgag gacgagcccg agcaggacgc
     2581 ccaccagcag gcctgccagg gggagaagca acggaaccgt cctcgctgcg gtgcgttccg
     2641 gcaaagggta aaccagcgga cccgagtgcg aacgcccggc tcgccatcag ggcttcctgg
     2701 tcggcttcgg aacgtacgtg ccgcccgact ccgacgcgag gatgatccgg gtgagcgtgc
     2761 cctccccgcc ggcgagcagg ctgcgcagca ggcggacctg gcccgggatc atgtccgtcg
     2821 ctcgcgtcga cgggctgtag aagccgaaga cggccgtcca ggacccggga gcattgacga
     2881 tgaacccgtc gtcgtcggtg accgccacct tcaggcgcgt cacggcgctg ccgacatcgt
     2941 cgggcgtcag gctgcccagg cgggtcgcga cgtcgaggtc gaccacatcg agcgtcgtgc
     3001 cgacggcgag cgtcgcgtcg gcaccgacgc gtcggtcctc gatcacggcg acccccgtcg
     3061 ggagcgggtc cccgtcgggc gcggtcgcga agatcgtgcc ctcgcgatcg gcgagatagc
     3121 gcgtgtcgcc gacctgccag gcgaggatcg gcacgcgctc ctcgatcgcg accaccacga
     3181 cggcgtcggg cagctggacg gtgacctcgg cgttcgcaac
//
LOCUS       DATBSW010000021         8524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1235561, whole genome shotgun sequence.
ACCESSION   DATBSW010000021 DATBSW010000000
VERSION     DATBSW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235561"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..323
                     /locus_tag="VIF63_00635"
     tRNA            239..323
                     /locus_tag="VIF63_00635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Leu,seq:tag)
     gene            complement(345..2318)
                     /locus_tag="VIF63_00640"
     CDS             complement(345..2318)
                     /locus_tag="VIF63_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8237917.1"
                     /translation="MHAFRRPPALGRFEPQTTLDKVAMSNFVPQLRSIDLLPMPAPGA
                     IGRPELDRVGRFFPALLWIILGAAVLETIGAIVFSQRVLAIAALLADVGALGVTFARF
                     LARNDRPLRGRVVLAATLTICGALGAYLVPDVGVSMALAPVMAVVLVLRYVDRNRLIA
                     VGATAVVSSLLILVADAAAGARAPLPDGVGTVFRDGVLMAVVLLLLLGLADWATEARD
                     SLRDMRASTERYLRVTTARLSIVSSLRVLHAQATPEATAQSIANALADLPLIDVAAIL
                     EVSSAGEALVIAIAGQADSPVQVGEKLPPARAAYIIERLHHGAWAEPWALRPTPTVND
                     QRFTDLGLKAQAYAPILFGDDIVGLVIIATTDPTEAAHLIADLPSVSEAAAVAGAILA
                     PSLMARRQTGAARVQIAGIIAERAFHPVFQPIIDLGTGLTVGFEALTRFANGDRPDQV
                     FSDAVKAGIGAELEAATLRAAVRYAAFLPADAWLSLNVSPSFLGAYAELAEILAGRNR
                     PITLEITEHDLIDDYPRLHDAMRALGSDVRLAVDDAGAGVANFRHLVNLRPDLVKIDA
                     GLIRGVEADVSRQALIVGLVHFAEVSGAQVLAEGVETELELDTVKRLGVTLAQGYLLA
                     RPAPVGRWTEAPAPVKAQPRRLPHPPMSAVLPN"
     gene            complement(2388..3485)
                     /locus_tag="VIF63_00645"
     CDS             complement(2388..3485)
                     /locus_tag="VIF63_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8237918.1"
                     /translation="MRALRSLSLANLRSFTRDRAALFWTLAFPIVFVILFGTIFSGGS
                     TNFTLGWVDEDGSPPAAALRAAFVDNAPVELVNGTLEESKARMQAGDLDGILVIPSGL
                     GAAIAAGQGGQAGPPVAVTIITDPSRSTTAQAVQQIATGLVMAANLQLSGARPLLTVA
                     AETLQTNRLNGVSFFVPSILAMALMQLGVFAAIPLVQQREKLILKRLNATPLPRWVLV
                     GSNVVVRLIIAAAQTALIIGIGIAALGVEITGSWPVIIGFIVLGALTFTAVGYVIASF
                     TKTEEAASGVTQIVQLPLMFLSGIFFPLEVMPEWLRGFATFLPLTYLGDALRQVMVGG
                     TPFAPLGVDALVLAGWLVVSLGISARFFRWQ"
     gene            complement(3485..4432)
                     /locus_tag="VIF63_00650"
     CDS             complement(3485..4432)
                     /locus_tag="VIF63_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8237919.1"
                     /translation="MTTATQPSPPADAVIRVDDVRKAYGAVKAVDGVSFHVAKGEIFG
                     LLGPNGAGKTTTMEMIEGLHPPDSGELQVLGMDVRRQAAAIKARIGVQLQTAALYPQL
                     TVAELLALFGTFYAHHRPVDELLDALELGEKRNAQTKTLSGGQQQRLSVALALVNDPE
                     VVFLDEPTTGLDPAARQGLWNVVRGMKADGRTILMTTHYMEEAEALCDRLAIMDHGKV
                     LDHGTAEDLISRRFHERGVRFGRAGAPHKAQLEALPGVKRIVVEGDELVLYTSDVPAT
                     IAALLELTTRAGTEPENLMIRRASLEDVFLELTGRALRD"
     gene            complement(4454..5065)
                     /locus_tag="VIF63_00655"
     CDS             complement(4454..5065)
                     /locus_tag="VIF63_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237920.1"
                     /translation="MGARTDAARSEVLAARAGLDEELVRLEAAGRAAVDIPARLRREP
                     AKVLGAAGGAAFLLLGGPKRAFQGARRAIFGKGADIPKSMLPPEVEKTLKKLGPDGEK
                     VRGTLEREFASYLEEKAPQRRDRDLGATAAVLLGGAFKPVVERIGKQLAERALDPDGP
                     SFADGMRKARERARTNGTGTSATNGTSPTNGKTPPGAPDAKAR"
     gene            complement(5026..5901)
                     /locus_tag="VIF63_00660"
     CDS             complement(5026..5901)
                     /locus_tag="VIF63_00660"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8237921.1"
                     /translation="MDDDESTQSPGAPQRSRDDAAGTPPGLREQVGATFAAGKRLFQA
                     HIDLAKAEMSEIAGSVGRMVGLFAGAFVLMLFAGVLLFVGGILFLGEALFGSIGWGVL
                     LGLMLLIDLALVAVLLALDVSGKRVGTAFAAAAAIGAIVGLVLGFDLTHRGWEALGAA
                     VAPGVDAADRTRNLAVGSLAGIGAILGLLFGIRMGSRDGVVVRALVGAVGGLLIGALL
                     GWLGSITIGAQIGAAIGVLVALIAWPVLAGLDLARAGVDGEALKKKFTPQETITLTKE
                     TIEWVRARTPLVPKS"
     gene            6026..7222
                     /locus_tag="VIF63_00665"
     CDS             6026..7222
                     /locus_tag="VIF63_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY8237922.1"
                     /translation="MELIRTAELLSIGTELTVGETRDTNAGDLARDLTRRGVEVGRMS
                     ALPDELATVTAAFAEALERADLVVSTGGLGPTPDDLTREAIAGLVGETPVVDPALEVW
                     LRDLFERRSLPFPEANLKQAWLIPSSTPIPNEQGTAPGWYVARPDGRVVVALPGPPRE
                     MRPMWDGWVVPRLIESGLGRPIASVTLRLTGIGESAVASRLGSLLAAGGRPMVATYAR
                     ADAVDIRIWAHDEQGDEAAVLEDAASLVAGVERRIIDMLGDHVWGRGETSWPKAVADA
                     LDARGWRLAVAEVGLRGSLLALLGEGLAERLAFGESLVHRPEPHDGHRATLEHLAERV
                     RELGACDVGVAVEARERGGDTAVTVAVVTPESTHRETRVVFLGGAQGRSRAAVATAAI
                     LLARLR"
     gene            7320..8135
                     /locus_tag="VIF63_00670"
     CDS             7320..8135
                     /locus_tag="VIF63_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8237923.1"
                     /translation="MRLTILGTGSARPIGDSAQSGILVQAGETNVLFDIGSGIASQVE
                     RRVGATNLAGLFVGHFHADHWIDIGPMRYRFPWAEDAPRKLPVFLPAGGREKLAHLAA
                     AINERPTFFEPAFDITEYETGQRFEIGALTVTPHAVGHYVPAWSMDILGPEGERVVYA
                     GDMGPSEMVIDLARGAEVLILEATLEDGAKDDARRGHLDTPEAIDHVARSGVVQGLLV
                     HYWSERREIIRDLCEQSGVPVIPGLTGMVAEVSPGSVSIDGGAPRVRAGAILG"
     gene            complement(8179..>8524)
                     /locus_tag="VIF63_00675"
     CDS             complement(8179..>8524)
                     /locus_tag="VIF63_00675"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY8237924.1"
                     /translation="DVEKALAMFKRMSVPVLGLVENMTAFICPHCGEATEIFGRGGGE
                     RFAGQHQLEYLGGIPLDITVRQGGDVGVPAVAQREPGPAAKALTGIARTIAARMSVRA
                     AAANQQPVLTIS"
BASE COUNT         1269 a         2957 c         3093 g         1205 t
ORIGIN      
        1 gtgcttctcg accttttcct tctcctgccc cggcgcgccc gaaggctggc cggcgaagac
       61 cggtacggcg gcgacgacca gggcaagcag cgccatcgtc acgagggcaa gccgcggtcg
      121 ggaaaccatg cgggcagcgt actcccaccg ggcttgcatc gaacattgcg gcgccgtgcc
      181 ccggtgcgtt gcggcaccat ctcgcagggg gcaacctggc ctgtgggaaa ctggacgcgg
      241 gcgagtggcg gaatggcaga cgcgccggcc ttaggagccg gtgtcccgtg agggacgtga
      301 gggttcgacc ccctccttgc ccactctcac gcgcgggcgg ccgctcagtt cggcaggacc
      361 gccgacatcg gggggtgcgg caggcggcgc ggctgggctt tcaccggcgc tggcgcctcg
      421 gtccatcgcc cgaccggggc gggccgggcc aggaggtagc cctgggcgag ggtcacgcca
      481 agtcgcttga cggtatcgag ctcgagctcc gtctcgacgc cctcggccag cacctgggcg
      541 cctgacacct cggcgaagtg gacgaggccg acgatgaggg cttgccgcga gacgtcggcc
      601 tcgacgcctc ggatcaggcc ggcatcgatc ttgaccaggt cgggccgcag gttgacgaga
      661 tgccggaagt tggcgacgcc ggcgccggcg tcatcgacgg ccaggcggac atccgaacca
      721 agggcgcgca ttgcgtcatg cagcctggga tagtcgtcga tgaggtcgtg ctcggtgatc
      781 tcgagcgtga tcggacggtt ccgccctgcg aggatttcgg ccagctcggc ataggcccca
      841 aggaacgacg gtgacacgtt gaggctgagc cacgcatcgg ccggcaggaa ggctgcatag
      901 cggaccgcag cgcgaagggt ggccgcctcc agctccgctc cgatcccggc cttcacggcg
      961 tccgagaaca cttggtccgg gcgatcaccg ttcgcaaacc gggtgagcgc ctcgaagccg
     1021 acggtcaggc cggtgccgag atcgatgatc ggctggaaga cggggtggaa cgctcgctcc
     1081 gcgatgatcc ccgcgatctg gacgcgggcg gccccggtct ggcgtcgggc catcagcgat
     1141 ggggcgagga tggcacccgc gacggcggcc gcttcgctga ccgacggcag gtctgcgatg
     1201 aggtgcgcgg cctcggtcgg gtccgtggtc gcgatgatga ccagcccgac gatgtcgtcg
     1261 ccgaacagga tcggggcgta ggcctgcgcc ttgagcccga ggtcggtgaa tcgttgatcg
     1321 ttcacggttg gtgttgggcg tagcgcccac ggctcggccc aggcgccatg gtgaagtcgc
     1381 tcgatgatgt acgccgctcg cgccggcgga agcttctcgc cgacctggac gggactgtcc
     1441 gcctgccccg cgatggcgat gaccagggcc tcgcccgcgc tggacacctc gaggatcgcg
     1501 gcaacgtcga tgagcggaag gtcggcaagc gcgttcgcga tggactgggc cgtggcctcg
     1561 ggcgttgcct gcgcatgcag gacgcgcagc gagctcacga tggacaggcg tgctgtcgtc
     1621 acgcgcagat accgctccgt cgacgcccgc atgtcgcgca gcgaatcacg cgcctccgtc
     1681 gcccagtccg cgaggccgag gagcaggagc agcaccaccg ccatgagaac cccgtcacga
     1741 aacacggtcc cgacgccatc cggcaacggg gcccgcgcgc ccgcggccgc atcggcgacg
     1801 aggatcagta gggacgacac gacggccgtt gccccgacgg cgatcaggcg gttgcggtcg
     1861 acgtagcgca gcacgaggac gaccgccatg accggtgcca acgccatgct cacccccacg
     1921 tcggggacca gatacgcgcc caaggcgccg cagatggtca gcgtggcggc gagaacgacg
     1981 cggccccgca gcggccggtc gttgcgcgcc aggaaccggg caaaggtgac accgagcgcg
     2041 ccgacgtcgg cgagcaatgc ggcgattgcg agcactcgct ggctgaagac gatcgcgccg
     2101 atcgtctcga ggacagcggc accgaggatg atccacagca gggcggggaa gaagcgaccg
     2161 acccgatcga gctcgggccg gccgatcgcg cccggagccg gcatcggcaa caggtcgata
     2221 gatcgaagtt ggggcacgaa gttggacatg gccaccttgt cgagcgtcgt ttggggctcg
     2281 aaacgtccca gggccggcgg tcggcggaag gcgtgcacgg tgtgcggtat cgggcctggt
     2341 gtcaggctgg cggttcggga ccgccgggtc tataggccga attcccgcta ctgccagcga
     2401 aagaagcgcg ccgagatgcc gagcgagacg acgagccagc cggccaggac gagggcatcg
     2461 acacccagcg gcgcgaacgg cgtgccgccg accatgacct ggcggagcgc gtcgcccagg
     2521 taggtcaggg gcaggaacgt ggcgaagccg cgcagccact cgggcatgac ctcgagcggg
     2581 aagaagatgc ccgagaggaa catgagcggc agctgcacga tctgggtcac gcccgaggcc
     2641 gcctcctccg tcttcgtgaa ggacgcgatc acgtagccga cggccgtgaa cgtcagcgcg
     2701 ccgagcacga tgaagccgat gatcaccggc cagctccccg tgatctcgac gccgagcgcg
     2761 gcgatgccga tgccgatgat cagcgcggtc tgggccgcgg cgatgatcag ccgcacgacc
     2821 acgttcgagc cgaccaggac ccagcgcggc agcggcgtgg cgttgaggcg cttcaggatc
     2881 agcttctcgc gctgctgcac gagcgggatt gctgcgaata cgccgagctg catcagcgcc
     2941 atcgcgagga tgctcgggac gaagaacgag accccgttga gccggttcgt ctggagcgtt
     3001 tccgccgcga cggtcagcag cggccgggcc cccgacagct gcagattcgc ggccatcacc
     3061 aggcccgtcg cgatctgctg caccgcctgc gccgtcgtcg atcgactcgg gtcggtgatg
     3121 atcgtcactg ccacgggcgg tcccgcctgg ccaccctgac ccgccgcgat cgccgctccc
     3181 aggccactcg ggatcacgag gatcccgtcg aggtcgcccg cctgcatgcg tgccttcgac
     3241 tcctcgagcg tgccgttgac gagctcgacc ggtgcgttgt cgacgaacgc cgctcggagt
     3301 gcggccgcgg gtgggctgcc gtcctcatcg acccagccga gcgtgaagtt ggtgctgccg
     3361 cccgagaaga tcgtcccgaa caagatcacg aagacgatcg ggaacgcgag cgtccagaac
     3421 agcgccgcgc gatcccgggt gaagctccgg aggttggcga ggctgagcga ccgcagcgcg
     3481 cgcatcagtc gcgcagggct cggccggtga gctcgaggaa cacgtcctcg aggctcgcgc
     3541 ggcggatcat caggttttcg ggctccgtcc cggctcgcgt ggtgagctcc agcagggcgg
     3601 cgatcgtcgc gggcacgtcg ctcgtgtaca ggacgagctc atcaccctcc acgacgatgc
     3661 gcttgacccc gggcagcgcc tcgagctgcg ccttgtgggg cgcccctgca cgcccgaacc
     3721 ggacgccccg ctcgtggaag cgccgactga tcaggtcctc ggcggtgccg tgatcgagga
     3781 ccttcccgtg atccatgatc gccagccggt cgcacagcgc ctcggcctct tccatgtaat
     3841 gcgtggtcat gaggatcgtc cggccgtccg ccttcattcc ccggacgacg ttccacaggc
     3901 cctgccgcgc ggccggatcg agccccgtcg tcggctcgtc gaggaagacc acctcggggt
     3961 cgttgaccag ggccagcgcc acggacaggc gctgctgttg cccgcccgag agcgtcttgg
     4021 tctgggcgtt gcgcttctcg ccgagctcga gcgcgtcgag cagctcgtcc acgggccggt
     4081 ggtgcgcgta gaaggtgccg aagagggcaa gcagctcggc cacggtcagc tgcgggtaca
     4141 gcgccgcggt ctggagctgc acaccgatgc gcgccttgat cgcggccgcc tgccggcgca
     4201 cgtccatgcc gaggacctgg agctcaccgg agtcgggcgg gtgcaggccc tcgatcatct
     4261 ccatcgtcgt ggtcttgccc gcgccgttcg ggccgagcag accgaagatc tcgcccttcg
     4321 cgacgtggaa gctgactccg tcgacggcct tgaccgcgcc gtaggccttg cggacgtcat
     4381 cgacgcggat gacggcgtcg gccggagggc tgggctgggt tgccgtggtc acgcgaggct
     4441 ggttcgaagc ggctcagcgc gccttggcgt cgggtgcgcc tggtggcgtc ttcccgttcg
     4501 tcggactcgt gccgttcgtc gcgctggttc cggtgccgtt ggtgcgcgcg cgttcgcggg
     4561 ccttgcgcat gccgtctgcg aacgacggcc cgtcggggtc gagcgcccgt tcggcgagct
     4621 gcttgccgat ccgctcgacg accggcttga acgcaccgcc gaggagcacc gcggccgtcg
     4681 ctccgaggtc gcggtcgcgt cgctgcggcg ccttctcctc gaggtatgag gcgaactcgc
     4741 gctcgagcgt gccgcgcacc ttctcgccgt ccggcccgag cttcttgagg gtcttctcga
     4801 cttcgggcgg gagcatcgac ttcgggatgt ccgcaccctt cccgaagatt gcccggcggg
     4861 caccctggaa cgcgcgcttc gggccgccga ggagcaggaa cgcggcgccg ccagcggcac
     4921 ccagcacctt ggcgggctcg cgtcgcagcc gcgccgggat gtcgacggcg gcgcgcccgg
     4981 ctgcttccag ccgaacgagc tcctcgtcga gtcccgcccg cgcggctagg acttcggaac
     5041 gagcggcgtc cgtgcgcgca cccattcgat cgtctccttc gtgagcgtga tggtttcctg
     5101 cggggtgaac ttcttcttga gcgcctcgcc atcgacaccc gctcgagcaa ggtcgagacc
     5161 ggcgaggacc ggccaggcga tgagggcgac gagcacgccg atcgcggcgc cgatctgggc
     5221 gccgatcgtg atcgagccca gccagccgag gagcgcgccg atcagcaggc cgccaacggc
     5281 cccgacgagc gctcgaacca cgacgccgtc gcggctgccc atccggatcc cgaagaggag
     5341 gccgaggatc gcgccgatcc cggcgagtga gccgaccgcg agattcctgg tgcgatccgc
     5401 ggcatcgacc cccggcgcga cggcggcgcc cagcgcctcc cagccgcggt gggtgagatc
     5461 gaagccgagc accagcccga cgatggcgcc gatcgccgcg gcggccgcga acgcggtgcc
     5521 cacacgcttg cccgaaacgt cgagggcgag caggaccgcg accagcgcga ggtcgatcag
     5581 gagcatcagg ccgagcagga caccccagcc gatcgagccg aagagcgcct cgccgaggaa
     5641 caggatgccg ccgacgaaga gcaggacacc ggcgaacagc atgagcacga acgcgccggc
     5701 gaagagcccg accatccggc ccaccgagcc cgcgatctcg gacatctccg ccttcgccag
     5761 gtcgatgtga gcctggaaca ggcgcttgcc ggccgcgaac gtggcgccga cctgctcgcg
     5821 caggcccggc ggcgtgccgg cggcgtcgtc gcgcgagcgc tgcggtgctc ctgggctctg
     5881 ggtcgactcg tcgtcgtcca cgacacctcc gggggcttct gatctcgatc gatggtagca
     5941 gcggccgatc gcacgaccgc gatacgcgat cggcgcggcc atcgcgggcg cctcgcggtc
     6001 ccgccgagtc tcgtacgctc cggcaatgga gctgatccgg accgccgagc tgctgtccat
     6061 cgggacggag ctgacggtcg gcgagacccg ggacacgaac gcgggcgacc tcgctcgaga
     6121 tctcacgcga cgcggagtcg aggtcggtcg gatgtcggcg ctgccggacg agcttgcgac
     6181 cgtcaccgcg gccttcgccg aggcgctcga gcgtgccgac ctcgtcgtgt ccaccggtgg
     6241 cctcgggccg actccggacg acctcacccg ggaggcgatc gccgggctcg tgggcgagac
     6301 cccggtggtg gacccggcac tcgaggtctg gctgcgggac ctgttcgagc gccggagcct
     6361 gccgttcccg gaggcgaacc tgaagcaggc gtggctgatc ccctcctcta ccccgattcc
     6421 caacgagcaa ggcacggcgc cgggttggta cgtggcccgg ccggacggcc gggtcgtcgt
     6481 cgcgttgccc gggccgccgc gcgagatgcg cccgatgtgg gacggctggg tcgtgccgcg
     6541 cctgatcgag tccgggctcg gccgcccgat cgcctcggtc acgctccgcc tcaccggcat
     6601 cggcgagtcg gcagtcgcgt cgcgcctggg gtcgctgctg gctgccggcg gccgcccgat
     6661 ggtcgccacg tacgcacgcg ccgacgcggt cgacatccgg atctgggcgc acgacgaaca
     6721 gggagatgag gccgcggtct tggaggacgc ggcctcgctc gttgccggcg tcgaacgccg
     6781 gatcatcgac atgctcggcg atcacgtctg gggccgcggc gagacctcgt ggccgaaggc
     6841 ggtcgcggac gcgctcgatg cccgcggctg gcgcctggcc gtggcggagg tgggcctccg
     6901 cggatccctg ctggcgctgc tcggcgaagg cctcgcggaa cgcctggcat tcggcgaatc
     6961 gctcgtgcac cggcccgagc cgcacgacgg ccaccgggcg acgctcgagc accttgcgga
     7021 gcgggtgcgc gagctcggcg cgtgcgacgt cggggtcgcg gtcgaggcgc gggagcgcgg
     7081 cggcgatacc gcggtcacgg ttgccgtggt gacccccgag tcaacgcacc gcgagacgcg
     7141 cgtcgtattc ctcggcggcg cccagggccg cagccgcgcg gccgttgcga cggcggcaat
     7201 cctgctggcc cggcttcgat gagcgacccc tcgccgccca ggcccgcaag cccacggggc
     7261 ttggaccgac ccgctaccgt ggcgcctgca ccgcgccggg gggcagggtt ggaggatcga
     7321 tgcggctgac cattctcggc accggaagcg cccgaccgat cggcgacagc gcccagtccg
     7381 ggatcctggt gcaggccggc gagacgaacg tgctgttcga catcggcagc ggcatcgcct
     7441 cccaggtcga aaggcgggtc ggcgcgacca acctcgcggg cctcttcgtc gggcatttcc
     7501 acgccgacca ctggatcgac atcgggccga tgcgctaccg ctttccctgg gccgaggacg
     7561 cgcctcgcaa gctgccggtc ttcctgccgg cgggcggccg ggagaagctg gcgcacctcg
     7621 ccgcggcgat caacgagcga cccacgttct tcgagcccgc gttcgatatc accgagtacg
     7681 agaccggcca gcgcttcgag atcggagcgt tgaccgtcac gccgcacgcg gtcgggcact
     7741 acgtcccggc ctggtcgatg gacatcctcg gccccgaggg cgagcgcgtc gtctatgcgg
     7801 gtgacatggg tccgtccgag atggtgatcg acctcgcgcg cggcgccgag gtgctgatcc
     7861 tcgaagcgac gctggaggac ggcgcgaagg acgacgcccg gcgcggccac ctcgacacgc
     7921 cggaggcgat cgaccatgtc gctcggtcag gcgtcgtgca gggcctgctc gtccattact
     7981 ggtcggagcg gcgcgagatc attcgcgacc tgtgcgagca gtccggcgtg cccgtgatcc
     8041 ctgggctgac cggcatggtc gccgaggtct caccgggttc cgtctcgatc gacggtggcg
     8101 cgccgcgcgt gcgggcaggc gcgatcctcg gctagggaac ccggctgggg agcccggctc
     8161 gggctcggag ctcgtgggct aggagatcgt caggacgggc tgctggttcg ccgcggcagc
     8221 acgaacgctc atgcgcgcgg cgatggtccg cgcgatgccg gtcaaggcct tcgccgcggg
     8281 tcccggctcg cgctgcgcga ccgcgggcac gccgacgtcg ccaccctggc ggacggtgat
     8341 gtcgagcgga atgccgccga ggtactcgag ctggtgctgg cccgcgaacc gctcgccacc
     8401 gccgcgcccg aagatctcgg tcgcctcgcc gcagtgggga cagatgaacg cagtcatgtt
     8461 ctccacgagc ccgagcacag gcacgctcat gcgcttgaac atcgcgagcg ccttctcgac
     8521 gtcg
//
LOCUS       DATBSW010000022         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_495046, whole genome shotgun sequence.
ACCESSION   DATBSW010000022 DATBSW010000000
VERSION     DATBSW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495046"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VIF63_00680"
     CDS             complement(<1..790)
                     /locus_tag="VIF63_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8237925.1"
                     /translation="MIIDVHTHMYTRRWLELLRTSGDPYGIVVRPDGREEIFRGPTPV
                     VFPQPGHFDYGIRLEQMDRAGIDVSIVSLTCPNVYWGGEAVSTEAARDSNDSMAAAEH
                     AYPGRIKWFTSLPWQYPARAVEELVRSCDQGAAGVMVLANVAGRSLTEAAFEPIWTEI
                     DRRGLPVLVHPGEPPGADMLDMGRYDLSWSVGFMFDTTLAITRMVFDGFLDRFPNLRL
                     IVAHGGAALPYLVGRFAKGDEVELASRRRMTAHPLEYLRRMYYDC"
     gene            complement(787..1293)
                     /locus_tag="VIF63_00685"
     CDS             complement(787..1293)
                     /locus_tag="VIF63_00685"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8237926.1"
                     /translation="MAADPRAELVDRIEAIRDHWRQLVTEVGEDRMRLPGPMGEWTFK
                     DLASHLTAWRRRTIGRLEAAGRGEPKPPSPWPAELGEDEDDPINAWIHEHTKDDPLPR
                     VLAEADAAYEAVLAAVRALPLEVAARELESYVEAEADDGHPYGHLGTHEVDVRRWLDA
                     LEGDAQRR"
     gene            complement(1315..1391)
                     /locus_tag="VIF63_00690"
     tRNA            complement(1315..1391)
                     /locus_tag="VIF63_00690"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1355..1357),aa:Cys,seq:gca)
     gene            complement(1765..1838)
                     /locus_tag="VIF63_00695"
     tRNA            complement(1765..1838)
                     /locus_tag="VIF63_00695"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:Gln,seq:ttg)
     gene            complement(1871..1945)
                     /locus_tag="VIF63_00700"
     tRNA            complement(1871..1945)
                     /locus_tag="VIF63_00700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1911..1913),aa:Gly,seq:gcc)
     gene            complement(1974..2049)
                     /locus_tag="VIF63_00705"
     tRNA            complement(1974..2049)
                     /locus_tag="VIF63_00705"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2014..2016),aa:Phe,seq:gaa)
     gene            complement(2118..3182)
                     /locus_tag="VIF63_00710"
     CDS             complement(2118..3182)
                     /locus_tag="VIF63_00710"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8237927.1"
                     /translation="MATQRLVGDPFGSISEGVRSLLAVQSQDYAGAKWGLGQRVSGAT
                     DADLDRAFDAGALIRTHVLRPTWHFVAPEDLRWLQALTSPRIHQASGYQYRSNEIDTA
                     LAARAAAVFERELAGGQAKTREELGRALGEAGIAAAGLRQTYLVMHAELETIICSGPR
                     RGKRQTYALVEERVPPSRPRSRDEALAELAQRYVEGHGPAQAIDLAWWSGLTLGDARI
                     GLDLASPPLQRETIDGRVFFVSPAWPGLVAPMDNNPPIVHLLPNYDELLIAFRDRTDA
                     MDPDLPPPARIAAEILNHVIVRNGLGVGGWRRADDGRRARVDLNPLVPLSEADRQALR
                     AAVDRLSTFLGRPVETTGLD"
     gene            complement(3230..>4182)
                     /locus_tag="VIF63_00715"
     CDS             complement(3230..>4182)
                     /locus_tag="VIF63_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8237928.1"
                     /translation="AANRRNSFFLVLAVTALLAALGFTIGYGTTGYVEGAFGVTTIAI
                     VVAMLLSVGSYFAGDKLVLAASGAKEVTQESAPQLMNVVQEMALSAGVPVPKVYIIED
                     TAPNAFATGRDPKHASVAITTGLLQKLDREELQGVMGHELSHVRNFDIRFSLLVAVLV
                     GSIALLADFFLRFTFWGGGRRGGGDRDRGGGGLAAILFVVAIVLAVVAPFIARLVQLA
                     VSRQREYLADASAVELTRNTRGLESALAKIATDKEVLEVANRATQHLYFTNPIKKFEA
                     RSSAMFSTHPNVVDRINRLRQLSGEPELAEGDMAALAGLD"
BASE COUNT          652 a         1425 c         1477 g          628 t
ORIGIN      
        1 tgcagtcgta gtacatgcgc cgcaggtact cgagcgggtg ggcggtcatc cggcgccgac
       61 tcgccagctc gacctcgtcg cccttggcga agcgcccgac gagatacggc agtgcggcgc
      121 ccccgtgcgc cacgatcagc cgcaggttcg ggaatcggtc gaggaacccg tcgaagacca
      181 tgcgcgtgat cgcgagtgtc gtgtcgaaca tgaagccgac cgaccagctg aggtcgtatc
      241 ggcccatgtc cagcatgtcc gcacccggcg gttcgccggg gtggacgagg acgggcaggc
      301 cacgccggtc gatctcggtc cagatcggct cgaaggccgc ctcggtcagg ctccgacccg
      361 cgacgttggc gagcaccatc acgccggccg cgccctggtc gcagctgcga accagctcct
      421 caacggcccg cgcggggtac tgccacggca gcgaggtgaa ccacttgatc cggcccgggt
      481 aggcgtgttc tgccgccgcc atcgagtcgt tggagtcccg agccgcttcg gtgctcacgg
      541 cctcgccgcc ccagtagacg ttggggcagg tcagcgagac gatcgagacg tcgatcccgg
      601 cccggtccat ctgctcgagc ctgatgccgt agtcgaaatg gccgggttgc gggaacacga
      661 ccggcgtcgg ccctcggaag atctcctcgc gcccatccgg gcggaccacg atgccgtacg
      721 ggtcacccga cgtgcgcagg agctccagcc agcgccgggt gtacatgtgg gtgtggacgt
      781 cgatgatcaa cgccgctgtg cgtccccttc gagcgcgtcg agccagcgtc ggacgtcgac
      841 ttcgtgcgtg cccaggtggc cgtacgggtg gccgtcgtcg gcctcggctt ccacgtacga
      901 ctcgagctcc cgcgcggcga cttcgagcgg gagggcgcgg accgcggcga ggaccgcttc
      961 gtacgcagcg tcggcctccg ccagcaccct cgggagcgga tcgtccttcg tgtgttcgtg
     1021 gatccaggcg ttgatcggat cgtcctcatc ctcaccgagc tcggcgggcc acggcgaggg
     1081 tggcttcggc tccccacgcc cggccgcctc gagccttccg atcgtcctgc gacgccacgc
     1141 cgtgaggtgc gaggccaggt ccttgaacgt ccactcgccc atcggccctg gcagccgcat
     1201 ccggtcctcg ccgacctcgg tcacgagctg gcgccagtga tcgcggatcg cctcgattcg
     1261 gtcgacgagc tccgcgcgtg gatcggctgc catcgatgga tccctcggtt ggaatggagg
     1321 cgacgagcgg attcgaaccg ctgaatagag gttttgcaga cctccgtgtt gaaccacttc
     1381 accacgtcgc ctcggggccg ccggattctg ccacagggac gccggccatt gggttgatcg
     1441 ccccgatggg ccggacgttc gagatgcgga gcttgccggc gggcccgaaa ggcagcccga
     1501 tgatcgcccg gtgatcaaat ctgggggttc gtcgggcatt tcggctcggt gcggcgcaca
     1561 gagcggtccc ccagacccgg gtccaccctc acccgagcac ggatccaccc ccgacgacga
     1621 aagaaccgac cggcgagtgt gtgaaaaccg gcctgaaccg ccagaaatgg tcgcctcgtg
     1681 atcatcggcg accagatcgc gtgctgcggg ccagagggtc gaccgggctc agccgcgcag
     1741 acgaacgtgg ggattgagca gggttggttg cccctcgagg attcgaacct cgcctcacgg
     1801 attcaaagtc cgctgtccta ccactagacg aaggggcact gaccgccgtc agggtacgcg
     1861 ggcaaagaag tggagcggaa gacgggattc gaacccgcga ccctcacctt ggcaaggtga
     1921 tgcgctaccg ctgcgccact tccgctcgga ggagctgcgc ccgccggtgt tgttggtgcc
     1981 gagagccaga gtcgaactgg cgacaccgcg attttcagtc gcgtgctcta ccaactgagc
     2041 tatctcggcc cggcgagtcc gtcggtccga accggtccaa cggcgaggcg gaggatagca
     2101 cggggtccct tcggcgctca atcgaggccg gtcgtctcga ccggccggcc gaggaaggtc
     2161 gacagccgat cgacggccgc ccggagcgcc tgccggtccg cctcgctgag cggcacgagc
     2221 gggttcaggt cgacccgggc gcggcgccca tcgtcggcac ggcgccagcc gccgacgccg
     2281 aggccgttgc ggacgatcac gtgattcagg atctcggcgg cgatgcgcgc gggcggcgga
     2341 aggtccgggt ccatcgcatc ggtccggtct cgaaaggcga tcagcagctc gtcgtagttc
     2401 ggcagcaggt ggacgatcgg cgggttgttg tccattggcg caaccagacc gggccacgcc
     2461 ggcgagacga agaacacccg gccgtcgatg gtctcgcgct ggagcggcgg tgacgcgagg
     2521 tcgagcccga tgcgcgcatc gccgagggtg agtccggacc accaggcaag gtcgattgcc
     2581 tgggcaggtc cgtggccctc gacgtaccgc tgggcgagct cggcgagcgc ctcatcgcgc
     2641 gacctcggcc ggctcggcgg gacgcgctcc tcgacgagcg cgtaggtctg ccgcttccca
     2701 cgccgcgggc cgctgcagat gatcgtctcg agctccgcgt gcatgacgag gtacgtctgc
     2761 cggagcccgg cggcggcgat gcccgcctcg ccgagcgcac gaccgagctc ctcgcgcgtc
     2821 ttcgcctgac cgcccgcgag ctcgcgttcg aagaccgcgg cggcccgggc cgcgagcgcg
     2881 gtgtcgatct catttgaacg gtactggtag ccgctcgcct ggtggatgcg cgggctcgtc
     2941 agcgcctgca gccagcgcag gtcctcgggt gcgacgaaat gccaggtcgg gcgcagcaca
     3001 tgggtgcgaa tcagcgcgcc cgcgtcgaag gctcggtcga ggtcggcatc ggtcgcgcca
     3061 gagacgcgct ggccgaggcc ccacttggcg ccggcgtagt cctgcgactg cacggcgagg
     3121 agtgagcgga cgccctcgga aatcgagccg aacgggtcgc cgacgagccg ctgcgtggcc
     3181 aatcgccgcg cggcgacgga tgcctgtcca gcgccggcgg ggtccagggt cagtcgaggc
     3241 cagccagcgc cgccatgtcg ccctcggcga gctccggctc gccgctgagc tggcggagcc
     3301 ggttgatccg gtcgacgacg ttcggatggg tcgagaacat cgcgctcgag cgcgcctcga
     3361 acttcttgat cgggttggtg aagtacaagt gctgggttgc ccggttggcg acctcgagga
     3421 cctccttgtc cgtggcgatc ttcgccagcg ccgactccag gccgcgggtg tttcgggtca
     3481 gctcgacggc cgaggcatcg gcgaggtatt cccgctgccg gctgacggcg agctggacga
     3541 gtcgggcgat gaacggcgcc acgactgcga gcacgatcgc gacgacgaac aggatcgcgg
     3601 ccagtccccc acccccgcga tcgcgatcgc cgccaccgcg ccggcccccg ccccagaagg
     3661 tgaagcggag gaagaagtcg gcgagcaggg cgatcgagcc cacgaggacg gcgacgagga
     3721 gcgagaaccg gatgtcgaag ttgcggacgt gggacagctc gtggcccatc acgccctgga
     3781 gctcctcgcg atcgagcttc tgaagcagcc cggtcgtgat cgccaccgag gcgtgcttcg
     3841 ggtcgcggcc ggtcgcgaac gcgttcggcg cggtgtcctc gatgatgtag accttcggca
     3901 ccgggacgcc cgccgagagc gccatctcct ggacgacgtt catcagctgc ggcgccgact
     3961 cctgggtgac ctccttggcg cccgacgcgg caagcacgag cttgtcaccc gcgaagtagg
     4021 agccgaccga cagcagcatc gcgaccacga tcgcgatcgt cgtcacgccg aacgcgcctt
     4081 ccacgtaccc ggtcgtgccg tagccgatcg tgaagccgag ggccgcgagc agcgcggtca
     4141 cggccaggac gaggaagaac gagttgcgcc ggttcgccgc ct
//
LOCUS       DATBSW010000023         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1670, whole genome shotgun sequence.
ACCESSION   DATBSW010000023 DATBSW010000000
VERSION     DATBSW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VIF63_00720"
     CDS             complement(<1..142)
                     /locus_tag="VIF63_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8237929.1"
                     /translation="MAKKKFERTKPHVNIGTIGHIDHGKTSLTAAISKYLALKGSAEY
                     RSF"
     gene            complement(177..2255)
                     /gene="fusA"
                     /locus_tag="VIF63_00725"
     CDS             complement(177..2255)
                     /gene="fusA"
                     /locus_tag="VIF63_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8237930.1"
                     /translation="MPRTVPLARTRNIGIIAHIDAGKTTVTERILYYTKKIYKIGEVH
                     EGAATMDWMPQEQERGITITAAATTCFWNDHKINIIDTPGHVDFTVEVERSLRVLDGA
                     VVVFDGVAGVEPQSETVWRQADKYSVPRICFINKLDRTGADFWRCVDMIVDRLGARPV
                     PIQLPIGSEDRFEGVIDLIEMKAILYRDDLGSKIDVVEIPEALKDDAEKWREKMIEVV
                     AEADDTLTHKYLEGEALTTAEIRHGLRLGTLQNKFVPVLTGSALKNKGVQPMLDAVTE
                     FLPSPLDVPPTIGLRPGTTEELVRTVDDKEPMSGLVFKIATDPFVGHLAFFRVYSGTL
                     KAGSYVLNSAKGKKERVGRALEMHANHREEISEIFAGDIGAIVGLKESYTGDTLCDPD
                     HPILLENISFPEPVIEVKVEPRTKADQDKMGIALQRLAEEDPTFRVHTDPESGETLIA
                     GMGELHLDVIVDRMVREFKVAANVGKPQVSYRETIRRPAEGNGRFVRQTGGKGQYGHV
                     ILTAEPGEKGSGYEFVDKIVGGTIPREYMRAINMGIGEALGTGIYAGYPMVDVKVTVH
                     DGSYHEVDSSEMAFKIAASMAIKDAVEKASPVVLEPMMRVEVTMPEQFMGDVIGDLNS
                     RRGQVDGMESRGTTQVVRAFVPLAEMFGYATDLRSMTQGRASYSMELSHYSEVPGNLA
                     QELVAKSKVS"
     gene            complement(2273..>2632)
                     /gene="rpsG"
                     /locus_tag="VIF63_00730"
     CDS             complement(2273..>2632)
                     /gene="rpsG"
                     /locus_tag="VIF63_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY8237931.1"
                     /translation="LAERILRQALARAEASARRPGLEVMETALRNATPIIEVKPRRVG
                     GATYQVPVEIRGDRRLSLGVRWLVQAARKRSGKSMSEKLAAEFVDASNGLGAAVKRRE
                     DTHKMAEANKAFSHFKW"
BASE COUNT          364 a          847 c          919 g          502 t
ORIGIN      
        1 cgaagctgcg gtactcggcc gagcccttca gcgcgaggta cttgctgatc gccgcggtca
       61 ggctcgtctt gccatggtcg atgtggccga tcgtgccgat gttcacgtgc ggcttggttc
      121 gctcgaactt cttcttggcc atcgttggtt tctcctggct ctcggtcggg gccggctcag
      181 ctgaccttgg acttggcgac gagctcctgg gcaaggttgc ccgggacctc tgaatagtgc
      241 gaaagctcca tcgagtagct ggcccggccc tgggtcatcg accgcaggtc cgtggcatac
      301 ccgaacatct cggcgagcgg cacgaacgcg cgcacgacct gggtcgtgcc ccgcgattcc
      361 atgccgtcga cctggccgcg tcggctgttg aggtcgccga tcacgtcgcc catgaactgc
      421 tccggcatcg tgacctcgac ccgcatcatg ggctcgagca cgaccgggct cgccttctcg
      481 acggcatcct tgatggccat cgaggcggcg atcttgaacg ccatctccga tgagtcgacc
      541 tcgtggtacg acccatcgtg gacggtgacc ttgacgtcga ccatcgggta gccggcgtag
      601 atgccggtgc cgagggcttc gccgatgccc atgttgatcg cgcgcatgta ctcgcgcggg
      661 atggtgccgc cgacgatctt gtcgacgaac tcgtagccgg agcccttctc gccgggctcg
      721 gcggtgagga tgacgtggcc gtactggccc ttgccgccgg tctggcggac gaaccggccg
      781 ttgccctcgg cggggcggcg gatcgtctcg cggtacgaga cctggggctt gccgacgttc
      841 gcggcgacct tgaactcgcg gaccatgcgg tcgacgatca cgtcgaggtg cagctcgccc
      901 atgccggcga tgagcgtctc gccgctctcc gggtccgtgt ggacgcggaa ggtcgggtcc
      961 tcctcggcga ggcgctggag ggcgatgccc atcttgtcct ggtcggcctt cgtgcgcggc
     1021 tcgaccttga cctcgatgac cggctcgggg aacgagatgt tctcgagcag gatcgggtga
     1081 tccgggtcgc acagggtgtc gccggtgtag gactccttga gcccgacgat ggcgccgatg
     1141 tcgccggcga agatctcgct gatctcctcg cggtggttgg cgtgcatctc gagggcgcgc
     1201 ccgacgcgct ccttcttgcc cttcgcgctg ttgagcacgt acgagccggc cttgagcgtg
     1261 cccgagtaga cccggaagaa cgcgaggtgg ccgacgaacg ggtcggtggc gatcttgaac
     1321 acgaggcccg acatgggctc cttgtcgtcg accgtgcgga cgagctcctc ggtcgtgccg
     1381 ggacggaggc cgatcgtcgg cggcacgtcg agcggcgacg gcaggaactc ggtgaccgcg
     1441 tcgagcatcg gctggacgcc cttgttcttg agcgccgaac cggtcaggac gggcacgaac
     1501 ttgttctgga gcgtcccgag gcggagaccg tggcggatct cggcggtcgt gagcgcctcg
     1561 ccttcgaggt acttgtgggt gagcgtgtcg tccgcctcgg cgacgacctc gatcatcttc
     1621 tcgcgccact tctcggcgtc gtccttgagc gcctcgggga tctcgacgac gtcgatcttc
     1681 gagccgaggt cgtcccggta caggatcgcc ttcatctcga tgaggtcgat gacgccctcg
     1741 aagcggtcct cgctgccgat cggcagctgg atcggcacgg gccgcgcgcc caggcggtcg
     1801 acgatcatgt cgacgcagcg ccagaagtcc gcaccggtgc ggtcgagctt gttgatgaag
     1861 cagatgcgcg gcaccgagta cttgtcggcc tggcgccaga ccgtctcgga ctggggctcg
     1921 acaccggcga cgccatcgaa gacgacgacc gcgccatcga ggacccggag gctgcgctcg
     1981 acctcgacgg tgaagtccac gtgcccgggc gtatcgatga tgttgatctt gtggtcgttc
     2041 cagaaacagg tcgtggccgc ggcagtgatg gtgatgccgc gctcctgctc ctgcggcatc
     2101 cagtccatcg tggccgcgcc ctcatggacc tcaccgatct tgtagatctt cttggtgtag
     2161 tagaggatcc gctcggtcac ggtcgtcttg cccgcgtcga tgtgggcaat gatcccgatg
     2221 ttcctggtcc tggcgagcgg gacggttcgt ggcatctgct actggtcccg gactaccact
     2281 tgaaatggct gaaggccttg ttggcctccg ccatcttgtg ggtgtcctcg cgccgcttga
     2341 cggcggcgcc caggccgttc gaggcgtcaa cgaactcggc ggcgagcttc tcgctcatgc
     2401 tcttgccact gcgcttgcgg gcggcctgga cgagccagcg gacgccgagc gacaggcggc
     2461 gatcgccccg gatctcgacc gggacctggt aggtcgcgcc accgacgcgg cggggcttga
     2521 cctcgatgat cggggtggcg ttgcgcagcg cggtctccat gacctcgagg ccgggccggc
     2581 gcgcggaagc ctcggcgcgg gcgagcgcct ggcggaggat gcgctcggcg ag
//
LOCUS       DATBSW010000024         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_36984, whole genome shotgun sequence.
ACCESSION   DATBSW010000024 DATBSW010000000
VERSION     DATBSW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36984"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VIF63_00735"
     CDS             <1..467
                     /locus_tag="VIF63_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8237932.1"
                     /translation="IIVDRHRVSMVRVAFVVCGDADLAEESVQAAWPIAWQRLASVRD
                     PDRLGAWLASVAVNEARQLMRRRRRRAVIEIAVATHERSTAPDPSASVDDIDLARALA
                     NLEPEDRAILALRYVAGLNSSELGPAVGMSASGLRRRLARLLDRLRSELSDA"
     gene            460..1695
                     /locus_tag="VIF63_00740"
     CDS             460..1695
                     /locus_tag="VIF63_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237933.1"
                     /translation="MHEPEMTPFEIQLASRLRAYSAIEVAPASGLAIARDAGLHRRGA
                     AFTRLPARGPLRLALVAALAAALIGAALFLAGRQPSLPPVSPPASAPVPTAPSPSPAS
                     LDATLEGTWLADKPGNLSFGDPSGPARMALVFDANATAAFVTVSSSGERARLTTFVSA
                     ATEGLRFEERLESDPVVVDGIGLPACPAGGAGTYTLQRSADGQFMTLASAVDACAGRS
                     AVLARTWVRSLAAASGGGPGVVDALSVLFSVSMPNGSYAIDRSPGSITIAQPNPELQL
                     MVIEDPQGFNDPCDAAAGRYVIEPGADAFVAYFEQLEGFTVDSTAEALVDGHRAIRLV
                     EHADLDAPRCPSGDRLEWQPRVETSRSWFLPAGVTDSLYIVELPGSTLLFEVLPAPNA
                     VEDEIIGSIRFHDELPAGP"
     gene            1789..3015
                     /locus_tag="VIF63_00745"
     CDS             1789..3015
                     /locus_tag="VIF63_00745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8237934.1"
                     /translation="MTAGHLASRVALAAVFAIVAGCGSGVPSLRPGSATTPTVATTPT
                     ENPTVAPTDPPPTRAPWTLFPAYPTATLDGATAGKLQAILDDLVSGGYPDAVAAVVTA
                     DGTWAGAAGIDGPDGRLADPSDMFNIASVSKPILAALVLRLAQDGKVDLDAPLANYLD
                     EVPVVANGATVRQALQMRAGYGDTPASAAEASSADCRRVWSRADTLVSLPETATKPGG
                     IFRYSNPSYKLLGYAAEEVSGMPLDAAFDEQLFGPLGLDRILLQTADRKTPKPWAVPI
                     GKYGGGLDLDAFGTGGTLPCVSLSTFSFSTSAVASDAPSLARWAWGLFAGELIDRSAL
                     EQMTTPFDGGHAMGIELLPDFRPNLAYGVHGGNAGYAAFLTVLPERQAVAVVFVNFQE
                     AEVQGAARDLLLALAK"
     gene            3099..>3399
                     /locus_tag="VIF63_00750"
     CDS             3099..>3399
                     /locus_tag="VIF63_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY8237935.1"
                     /translation="MDKRALVERAGRGDHDAFAVLATAASARLDAAARLILRDPELAR
                     DAVQNTLVRAWRDLPGLRDPDRFDAWLHRLLVNASLDEARRRRRRPIEVEIKTIGE"
BASE COUNT          474 a         1233 c         1157 g          535 t
ORIGIN      
        1 cgatcatcgt cgaccgccat cgggtctcga tggtccgcgt ggccttcgtg gtctgcggcg
       61 atgccgattt ggccgaggag tccgtgcagg cggcctggcc gatcgcctgg cagcgtctcg
      121 catcggttcg ggatccggat cgccttggcg cgtggctggc ttctgtcgcg gtcaacgagg
      181 cccgtcagct gatgcgtcgc cgccggcggc gggcggtgat cgagatcgca gtcgccacgc
      241 acgaacgatc gaccgccccg gacccatctg catcggtgga cgacatcgac ctcgcccgtg
      301 ctctcgcgaa cctggagccc gaggatcggg cgatcctcgc ccttcggtat gtcgcgggcc
      361 tcaactcctc ggagctcggt cccgcggtcg ggatgtcggc atccgggctg cgccgccgcc
      421 tggcgcgcct gctcgatcga cttcgatcgg agctcagcga tgcatgagcc cgagatgacc
      481 ccgttcgaga tccagctcgc gagccgcctc cgcgcctact cggcgatcga ggtcgcgccc
      541 gcgtccgggc tcgcgattgc gcgagacgcc ggattgcacc gacgtggcgc tgccttcacc
      601 cgcctcccag cgcgcggtcc gctgcgcctt gccctcgtcg ccgcgctcgc ggcggcgctg
      661 atcggcgcgg cgctgttcct cgccgggcgg cagccgagcc tgccgcctgt ttcgccgccg
      721 gcgagcgctc cagtcccgac cgcgccgagc ccaagccctg cgagcctcga tgccacgctc
      781 gaagggacgt ggctcgccga caagcccggc aacctgtcgt tcggcgaccc gtccgggccg
      841 gcgcggatgg cgctggtgtt cgatgccaac gcgacggcag ccttcgtgac ggtttcgtcg
      901 agtggcgagc gcgcacggct cacgaccttt gtctccgcgg cgaccgaggg tctgcggttc
      961 gaggagcgcc tcgagagcga tcccgtggtc gtcgatggca tcggtctgcc cgcatgtccg
     1021 gcgggtggcg ccgggaccta cacgttgcag cgatcggcag acgggcagtt catgacgctt
     1081 gcctcggcag tcgacgcctg cgccggacgt tcggcggtgc tcgcccggac gtgggtgcgt
     1141 tcgctggctg ccgcgagtgg cggaggcccc ggcgtcgtcg atgccctgtc cgtgctgttc
     1201 tccgtgtcca tgccgaacgg cagctacgcc atcgatcgca gtcccggctc gatcacgatc
     1261 gcccagccga accccgagct gcagctcatg gtcatcgagg acccgcaggg cttcaacgat
     1321 ccgtgtgacg cagccgcggg gcgatacgtc atcgagccgg gcgccgacgc cttcgtcgcg
     1381 tacttcgagc agctcgaggg cttcaccgtc gactcgaccg ccgaggccct cgtggacgga
     1441 caccgcgcga tccggctcgt ggagcacgcc gatctcgacg ctccacggtg cccgtcaggc
     1501 gaccgcctgg aatggcagcc gcgggtggag acgtcccgca gctggttcct gccggcggga
     1561 gtcaccgata gcctgtacat cgtggagctc ccgggctcga ccctgctctt cgaggtgctc
     1621 ccggcgccca acgccgtcga ggacgagatc atcggctcca tccgattcca cgacgagctc
     1681 cctgccggcc cctgaccggc gaacccggaa tcacttgggc cgggcccgcg catctacgtc
     1741 gtgagccgcc aaagtgttgc tactcggcgg gcagtcaggg agtctgcaat gaccgccggt
     1801 cacctcgcat ctcgcgtggc gctcgccgcc gtcttcgcca tcgtcgcggg ctgtgggtcg
     1861 ggtgtaccca gcctcagacc aggcagcgcc accactccga cggttgccac gaccccaacg
     1921 gagaacccga cggttgcgcc gaccgatcca ccgccgacca gggcaccgtg gacgctgttc
     1981 cccgcctatc cgacagcgac gcttgacggc gccactgcgg ggaagctcca ggcgatcctc
     2041 gacgatctgg tttcaggcgg ctacccggat gcggtcgcgg cggtcgtgac cgctgacggg
     2101 acatgggccg gcgcggccgg catcgacggg ccggatggcc gcctggcgga tccgagcgac
     2161 atgttcaaca tcgccagcgt cagcaagccg atcctcgccg ccctcgtcct ccggcttgcc
     2221 caggacggca aggtcgatct cgacgcgccg ctcgcgaact acctcgacga ggtcccggtc
     2281 gtcgcgaacg gggcgacggt tcgacaggcg ctccagatgc gggcgggcta cggcgacacg
     2341 ccagcgagcg cggcggaggc atcgagcgcg gattgccgtc gggtctggtc cagggccgac
     2401 accctcgtgt cgctgcccga gaccgccacc aagccgggcg ggatcttccg atactcgaac
     2461 ccgtcctaca agctcctcgg ctacgccgcc gaggaggtct ccgggatgcc cctcgacgcc
     2521 gccttcgacg agcagctgtt cggcccgctc ggcctcgacc ggatcctgct ccagaccgcc
     2581 gatcggaaga cgcccaagcc gtgggcagtg ccaatcggaa agtacggcgg cggcctggat
     2641 ctggatgcgt tcgggaccgg tgggacgctg ccctgcgtct ccctctcgac gttctcgttc
     2701 tcgacatcgg ctgttgccag tgatgcgccg agcctcgccc gctgggcatg gggcctgttc
     2761 gccggggagc tgatcgatcg ctccgccctc gagcagatga cgacgccatt cgacggtggc
     2821 cacgcgatgg ggatcgagct gctgcccgac ttccggccga accttgccta cggtgtccac
     2881 ggcggcaacg ccggctacgc agcattcctc accgtgctgc cggaacggca ggcggtcgcg
     2941 gtcgtcttcg tgaacttcca ggaagccgag gtccagggcg cggcacgcga cctgctcctg
     3001 gccctggcga agtagcggcg ccgcgaaacg attcgcgcac cggtgacgcc ataccctgcg
     3061 taacccggtc atcgtggccg gggcgagggt gagggaccgt ggacaagcgg gcactcgtcg
     3121 agcgagccgg aaggggcgat cacgacgcct ttgccgtgct cgccacggcg gcgtcagccc
     3181 ggctggacgc ggccgcgcga ctcatcctcc gggacccgga gctcgcgcgc gacgcggtcc
     3241 agaacaccct ggtgcgagcc tggcgggacc tgcccggact tcgtgatccg gaccggttcg
     3301 atgcctggct ccaccgactc ctcgtcaatg ccagcctcga cgaggcccgc cggcgacggc
     3361 gacgcccgat cgaggtcgaa atcaagacca tcggcgaac
//
LOCUS       DATBSW010000025         6825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_37148, whole genome shotgun sequence.
ACCESSION   DATBSW010000025 DATBSW010000000
VERSION     DATBSW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37148"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VIF63_00755"
     CDS             <1..201
                     /locus_tag="VIF63_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237936.1"
                     /translation="ARSLKANALWQFSERDFFIAPMSAVELARAAPGDPVIEWYQADH
                     AMRSAKARAARRAFLARELGLG"
     gene            505..601
                     /locus_tag="VIF63_00760"
     tRNA            505..601
                     /locus_tag="VIF63_00760"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:539..541,aa:Sec,seq:tca)
     gene            complement(642..3098)
                     /locus_tag="VIF63_00765"
     CDS             complement(642..3098)
                     /locus_tag="VIF63_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8237937.1"
                     /translation="MTAVAARAERLDYTALGDRMLVLLAMRAAFATLVVAWSASRPDL
                     LGMSLTQLGSASVAYLLLMTGAELTRRRFPRYGVLVLSLTLLIDGVFLAMASYVTGGT
                     QSPIRFLTYLHLVAVSLLASYRTGLKVALWHSLLLFVVLYAQAAQLLPAVDVVPGREI
                     EFNQMPVLNVTSFWLFALATSLFSGMNERELRNRRSDLQSLVDISARLDAVTDPVVQS
                     RILLDGLAERFGFEQGLIVGQTDGQVIVLASRHTVQLPTTALAPDQVIQRAWDTLELL
                     PVKRIDAKRNPLLATAFGEGCNVLVAPMLADGRALGAVVVPHRGRRQLGLERRIANVL
                     GQLCSMAALNLRNAVLLRDIQDLAERDSLTGAANRRMFNMTLERVLSTSPRRTTTAHG
                     GVTAVLFVDLDDFKVVNDTLGHAAGDALLVAVTERIVGAVRATDLVARLGGDEFAILT
                     DDGADLQRSVAMAERLVRDLRLPYLIDGHHVVVTVSIGIASTRDTAENASDVVRNADL
                     AMYIAKANGKAGYAVFDPGMHTVIRDRHELGTQLLGAVDLGQLRVIYQPIVSLVTGST
                     EGVEALVRWDHPERGLVSPGEFIEIAEENGAILPIGRWVLAEACRQARSWGTGEHAPF
                     LCVNVSAREIQQPDFVDAVERTLTEAGFEPSRLSLEITETALLRATPTTVNTLEALRG
                     LGVRILIDDFGTGYFSLSHLRQFPVDGLKIASEFVQVSAADAKTVALASAIVAMSSSM
                     QILTVAEGIENREQAERMRALGCTYGQGYYFARPQAAADILLTGQALPVATPARVPAA
                     VRPRQAAIFPGRTILAPKLA"
     gene            complement(3095..4513)
                     /locus_tag="VIF63_00770"
     CDS             complement(3095..4513)
                     /locus_tag="VIF63_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237938.1"
                     /translation="MPIVRKRRGPLALSISAALLAGMLAVGLSGGPATAAAPVRVDFG
                     PEPAVAAINLPVTLTATVVDAVGTPIVGVSVRVYFTGGPNDPGGNGSSPDMTCTTGAA
                     GSCVVAYTPTLTGTDTLCAQMSGSPHACTEPADNPELDDNMDSIEVVVTTTLPTSTPA
                     PTVAPTPEPTVAPTVEPTVAPTLEPTLEPTVAPTSEPTVPPTPERTAAPTAAPTAAPT
                     AAPTAAPTVAPTAAPTAAPTVAPTAAPTAAPTVAPTAAPTAAPTVTPTVAPTISPTVA
                     PTAAPTGGPTVTPTLQPTAAPTTTDEPTAGSTPSPDATVQPDATLVPDSTLGPDSTLV
                     PDSTLGPDSTLVPDPTLVTDPTLTPAPSLTAIGGAGSGGTSSGGGSTTTSSGGGAIDE
                     PATNGGGIVEAIGEVVDQIGAALKPGVVAVVATTFTFPLLLALLVLLFLIVQHRLDER
                     DPKLRHAPSSAFDTYIAFEEDR"
     gene            4817..6226
                     /gene="selA"
                     /locus_tag="VIF63_00775"
     CDS             4817..6226
                     /gene="selA"
                     /locus_tag="VIF63_00775"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY8237939.1"
                     /translation="MRPPSVDAVLRAAAPGLADRDRTMVVVLARSVIADERKGLADGA
                     LARSPSELAAELLQRLAGLEGPGAVAAGAGAGTGAAASSGLTSVINATGVLLHTNLGR
                     AAWPAAAIEAARLAASAPSMLELDEDTGRRGRRFRAIEDEVVALTGAEDALVTNNNAA
                     AVALAVGLAGRGGVAVSRGELVEIGGGVRIPEIVRRAGARLVEVGTTNRTRVADFEAA
                     LADGRAKLVLRVHPSNFAQTGFVETPDAAALASVAHAHGAIVVDDLGSGALLDTERFG
                     LAHEPTPRERLEAGADLVTFSGDKLVGGPQAGIIAGRADLIEKLRRDPLARAMRPDKV
                     ILAALAATLRLYRAGVAQAEIPVWRQVCTPVAALEVRARRLAEATGTRVTATESTIGG
                     GSLPGQVLGSRAIAIDGPSARRTLDALRSGDPPVIGRIVDDVVLLDLRTVEPVDDDRL
                     AGAVRAAIAAGSTESRSRA"
     gene            6231..>6825
                     /locus_tag="VIF63_00780"
     CDS             6231..>6825
                     /locus_tag="VIF63_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8237940.1"
                     /translation="MPAPTVVIGTAGHIDHGKTSLLRALTGIDADRLPEEQARGMTID
                     VGYAHMAFEDGVELDFVDVPGHDRLIGNMLVGAGEIDAAMLVVAADDGPRPQTIEHLQ
                     LLDALEVTDGLVVITKSDLVDAARLAEVRGAVAALVAGTSFAGVPLVVVSAFSGEGVA
                     DLRAALRSVRDRVSTQRSLAPAAGKGGPARLAIDRAFA"
BASE COUNT          995 a         2311 c         2526 g          993 t
ORIGIN      
        1 gcgcgctcgc tcaaggcgaa cgccctgtgg cagttcagcg agcgcgactt cttcatcgcc
       61 ccgatgtcgg cggtcgagct ggcgcgggcg gccccgggcg acccggtgat cgagtggtac
      121 caggccgacc acgcgatgcg cagcgcgaag gcgcgggccg cccgccgggc cttcctcgct
      181 cgggagttgg gcctgggctg aacttgctga cgatgatcat ggggtgacca tttctggggg
      241 ttcgcgccgc atttggggcg ccgcgcgtgt cggacgaggg ccgctgctcc tttagccccg
      301 atccataagc acgattcgag cgcgccgtgc gccgtccaga cagcccaaag ggtcgatcgt
      361 gcccttgttc ggacagaaac agcccgcaga ctcccggaaa tgatcatgcc gtgatcttcg
      421 aatgggaatt cggcctatcc gagctcggca gctggacctt gtttggcccg cgttcgcaat
      481 ctcggcgcct cgatacgatg acgcgggagt gcgttcgggc cggtgcccgg cttcgtcttc
      541 aaaacgaatg gcgcggcgct cggcgtcgca cggtgggttc gactcccatg cgctcccgcc
      601 accgcagcgg ctcgtcgtcc ccggcccgag gatcgcccgg ctcaggccag cttcggcgcg
      661 aggatcgtgc ggcccggaaa gatcgcggcc tgtcgcggcc ggaccgcggc cggcacacgg
      721 gcgggggtcg ccaccggcag cgcctgcccc gtgaggagga tgtcggcagc ggcctgcggc
      781 cgggcgaagt agtagccctg gccgtacgtg caacccagcg cacgcatgcg ctcggcctgc
      841 tcccggttct cgatgccctc cgcgaccgtg aggatctgca tcgatgagct cattgccacg
      901 atcgcgctgg cgagggcgac cgtcttggcg tcggctgccg acacctgcac gaactcgctc
      961 gcgatcttga gcccgtcgac cgggaactgg cgcaggtgac tcaaggagaa gtagccggtg
     1021 ccgaagtcat cgatgaggat gcgaaccccg agcccgcgca gggcttcgag ggtgttgacc
     1081 gtcgtcggcg tggctcgcag gagggccgtc tcagtgatct cgagactcag ccgcgatggc
     1141 tcgaacccgg cctccgtcag cgtccgctcg acggcgtcga cgaaatcggg ctgctggatc
     1201 tcgcgggccg agacattgac gcacaggaac ggagcgtgct cgccggtgcc ccagctgcgc
     1261 gcctggcgac aggcttcagc caggacccac cgaccgatcg gcagaatcgc gccgttctcc
     1321 tcggcgatct cgatgaactc gccggggctg acgagcccgc gctctggatg gtcccagcgg
     1381 acgagggcct cgacgccctc ggtcgagccc gtgacgagcg agacgatggg ctggtagatg
     1441 acccggagct ggccgagatc gaccgcgccg agcagctgcg ttccgagctc gtgtcgatcc
     1501 cggatcacgg tgtgcatccc cgggtcgaac accgcgtagc ccgccttgcc gtttgccttg
     1561 gcgatgtaca tggcgagatc ggcgttgcga acgacatcgg aggcgttctc ggcggtatcg
     1621 cgggtgctgg cgatgccgat gctcacggtc acgaccacgt gatggccatc gatcaggtac
     1681 ggcaggcgca ggtcgcgaac gaggcgctcg gccatggcga ccgatcgctg caggtccgcc
     1741 ccgtcgtcgg tcaggatcgc gaactcgtcg ccgcccaatc gggcgacaag gtccgtggcg
     1801 cgcacagcgc ccacgatccg ctcggtcacg gcgaccagca gggcgtcgcc cgctgcgtgc
     1861 ccaagggtgt cgttcacgac cttgaagtcg tcgaggtcga cgaacaggac ggcagtgacg
     1921 ccaccgtggg cggtcgtcgt gcgccgcggc gacgtcgaga ggacgcgctc gagagtcatg
     1981 ttgaacattc gccgattggc cgcgccggtc agcgaatcgc gctcggcgag atcctggatg
     2041 tcgcgcagga ggaccgcatt gcggaggttc agggctgcca tcgagcagag ctgaccgagc
     2101 acgttggcga tgcgccgttc cagcccgagc tggcggcgtc cgcgatgggg cacgacgacc
     2161 gccccgaggg ctcgcccgtc ggccagcatc ggcgcgacga gcacgttgca gccttcgccg
     2221 aacgcagtgg cgaggagcgg gttgcgcttc gcgtcgatgc gcttgaccgg cagcagctcg
     2281 agcgtgtccc acgcgcgctg gatcacctga tcgggcgcaa gcgcggtcgt cgggagctgg
     2341 acggtgtggc gcgaggccag cacgatgacc tggccgtcgg tctgcccgac gatgaggccc
     2401 tgctcgaagc cgaatcgctc ggcgaggcca tcgaggagga tgcgcgactg gacgacggga
     2461 tcggtgaccg cgtcgagcct ggcgctgatg tcaacgagtg actggaggtc ggaccgccga
     2521 tttcgcagct ctcgctcgtt catgcccgag aacagcgacg tggccagggc gaagagccag
     2581 aaagacgtga cgttgaggac cggcatctga ttgaactcga tctcccggcc gggcacgacg
     2641 tcgaccgccg gcagcagctg ggccgcctgg gcgtacagga cgacgaagag cagcagcgaa
     2701 tgccacagcg caaccttcag gccggtccgg tacgaggcga ggagcgagac cgccacgaga
     2761 tgcaggtacg tgaggaagcg gatcgggctc tgcgtgccgc cggtcacgta cgacgccatc
     2821 gccaggaaga cgccatcgat gagcagcgtc agcgacagca ccagcacgcc ataccgggga
     2881 aagcggcggc gcgtgagctc ggcgcccgtc atgagcagca ggtaggcgac cgacgcgctg
     2941 ccgagctgcg tgagcgacat cccgagcagg tcgggccgcg aggccgacca cgccacgacg
     3001 agcgtggcga acgcggcccg catcgccagc aggacgagca tccgatcacc gagcgcggtg
     3061 tagtcgagtc gttctgcgcg ggcggcgacg gcggtcatcg atcctcctcg aaggcgatat
     3121 aggtgtcgaa ggccgagctg ggcgcgtgcc gcagcttcgg gtcgcgctcg tccagtcggt
     3181 gctggacgat caggaacagg agcacgagca gggcgagcag gagcgggaag gtgaacgtgg
     3241 tcgcgacgac ggccacgacg cccggcttga gcgccgcacc gatctggtcg acgacttcgc
     3301 cgatcgcctc gacgatcccg ccgccgttcg tggccggctc gtcaatggcc ccgccgccgc
     3361 tggacgtcgt ggtcgacccg ccgcccgatg acgtacctcc agacccggcg ccaccgatgg
     3421 ccgtgagcga cggcgcgggc gtcaaggtcg ggtcggtcac gagcgttgga tcgggcacca
     3481 gggtcgaatc ggggccgagg gtcgagtcgg gcaccagggt cgaatcgggg ccgagggtcg
     3541 agtcgggcac cagcgttgca tcaggctgga cggtcgcgtc cgggcttggc gtggaccccg
     3601 cggtcggctc gtccgtcgtg gtgggcgcgg cggtcggctg aagggtcggt gtgacggtcg
     3661 gcccaccggt cggtgcagca gtgggcgcga ccgtcggaga aatcgttggt gcaaccgttg
     3721 gtgtaacggt gggcgcagcc gtgggcgcag cagtcggtgc aacagtcggt gcagccgtgg
     3781 gcgcagcagt cggtgcaaca gtcggtgcag ccgtgggcgc agcagtcggt gcaacggtcg
     3841 gtgcagccgt gggcgcagca gtcggcgcag ctgttggcgc cgcggtcggc gcggcggtcc
     3901 gctcgggcgt gggagggacc gtgggttccg atgtcggtgc gacggtcggc tcgagcgtcg
     3961 gctcgagcgt cggtgcaacg gtaggctcga ccgtcggtgc gactgtcggc tcaggcgtcg
     4021 gcgcgaccgt tggtgcgggc gtcgatgtgg gcagcgttgt ggtgacgacc acttcgatcg
     4081 agtccatgtt gtcgtcgagc tcggggttgt ccgccggctc ggtgcacgcg tgcggtgacc
     4141 ctgacatctg ggcacagagg gtgtccgtgc cggtcagcgt cggcgtatac gccaccacgc
     4201 agctcccggc cgcaccggtg gtgcacgtca tgtcggggct gctgccattc ccgcccgggt
     4261 cgttcggtcc gccggtgaag tacacccgca cgctgacgcc gacgatgggc gtcccgaccg
     4321 cgtcgacgac ggtggcggtc agggtcaccg ggaggttgat tgccgccacg gccggctcgg
     4381 ggccgaagtc gacgcgcacc ggcgcggccg ccgtcgcagg accgccggac aggcccacgg
     4441 cgagcattcc cgcaagcaac gctgcggaga tgctcagcgc cagtggtccg cggcgcttgc
     4501 gaacgatcgg cattcccgaa acccccagat gccggtggcc caacgcgggt gaagaacctc
     4561 ggtggcctgg ccccccggtc ggccccaagg cgtccgatcc actcgatcgg cagcaggacc
     4621 gtaggccgaa agtgccacgc cccaaaggga cgcagtagta cttgtcggtt aggtgaggtc
     4681 cctaggccgg tgcacgcgcg catcgccttg ccaacgcagg tcccagggcg cggatgaccc
     4741 atcggtacca gtgggcgtgc ggtccggagc ggtgcgggat cctccggtga tggcggcgcc
     4801 gcgtacgatg ccgggcgtgc gacccccgag cgtcgatgcc gttcttcgcg ccgccgcgcc
     4861 cggtctggcc gaccgggacc ggacgatggt cgtggtactc gctcgctcgg tgattgccga
     4921 cgagcggaaa gggctcgccg acggggcact cgcgcggtcc ccgtccgagc ttgccgccga
     4981 gctgctgcag cgcctcgccg gtctcgaggg acctggcgct gtcgccgcag gcgcgggcgc
     5041 gggaaccggc gccgccgcct cgtccgggct gacgtccgtg atcaacgcga ccggcgtcct
     5101 cctgcacacg aacctcgggc gcgccgcgtg gccggcggcg gccatcgagg cggccaggct
     5161 ggcagcctcg gcgccatcga tgctcgagct cgacgaggac accggccggc gtgggcgccg
     5221 cttccgggca atcgaggacg aggtcgtcgc cctgaccggc gcggaggacg cgctggtcac
     5281 caacaacaac gccgcggcgg tcgccctggc cgtggggctg gcggggcgag gtggcgtcgc
     5341 ggtctcacgc ggcgagctcg tcgagatcgg cggcggggtt cggatccccg agatcgtgcg
     5401 gcgggcgggc gctcggctgg tcgaggtggg gacgacgaac cgcactcgcg tcgccgattt
     5461 cgaggcggcg ctggccgacg gccgggccaa gctggtgctg cgcgtccacc cctcgaactt
     5521 cgcccagacc gggttcgtcg agacgccgga cgccgcggcc ctcgcgagcg tggcgcacgc
     5581 ccacggcgcg atcgtcgtcg acgacctcgg gtccggcgcg ctcctcgaca ccgagcggtt
     5641 cgggctggcc cacgagccga cgccgcgcga gcgcctcgaa gcgggcgccg acctcgtgac
     5701 cttctcgggc gacaagctcg tcggcgggcc gcaggccggg atcatcgccg gtcgtgcgga
     5761 cctcatcgag aagctccggc gcgacccgct cgcgcgcgcg atgcgtcccg acaaggtgat
     5821 cctcgccgcg ctcgccgcga cgcttcggct ctaccgggcc ggcgttgccc aggccgagat
     5881 ccccgtctgg cggcaggtct gcacgccggt ggccgccctc gaggtccgcg cgcggcgtct
     5941 cgccgaggcc accgggacac gtgtcacggc gacggaatcg acgatcggcg gcgggtccct
     6001 gccggggcag gtgctcgggt cgcgcgcgat cgcgatcgat gggccctcgg ccaggcggac
     6061 gctcgatgcg ctgcggtccg gcgatccgcc cgtcatcggg cgaatcgtcg atgacgtcgt
     6121 cctgctcgac ctgcgaacgg tcgaacccgt cgatgacgat cgattggccg gcgccgttcg
     6181 cgccgcgatt gcggcgggct cgaccgaatc gcgctcccgc gcctgagccc atgcccgcgc
     6241 cgaccgtcgt catcggcact gccggtcaca tcgatcacgg caagacgagc ctgctccgcg
     6301 ccctgaccgg aatcgatgcc gaccgactgc ccgaggagca ggcccggggc atgacgatcg
     6361 acgtcggcta tgcgcacatg gcattcgagg atggtgtcga gctcgacttc gtcgacgtgc
     6421 ccgggcacga ccggctgatc ggcaacatgc tcgtcggcgc cggcgagatc gacgcggcca
     6481 tgctggtcgt ggcggccgac gacgggccgc ggccacagac gatcgagcac ctccagctcc
     6541 tcgacgcgct cgaggtcacc gacgggctcg tcgtcatcac gaagagcgac ctggtcgacg
     6601 ccgcgcgcct cgccgaggtc cgaggtgcag tcgccgcgct cgtcgccggg acgtccttcg
     6661 ccggtgtgcc gctcgtcgtc gtatccgctt tctcgggcga gggcgtcgcc gacctccgag
     6721 ccgcgcttcg gagcgtccga gatcgcgtct cgacccagcg gagcctggcc ccggcagccg
     6781 gcaagggcgg ccctgcgcgg ctggccatcg accgggcgtt cgcgg
//
LOCUS       DATBSW010000026         7107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_72417, whole genome shotgun sequence.
ACCESSION   DATBSW010000026 DATBSW010000000
VERSION     DATBSW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72417"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VIF63_00785"
     CDS             <1..1572
                     /locus_tag="VIF63_00785"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8237941.1"
                     /translation="DFTGYVDLVHRKAFTWQGGKDVETQIPDGMADEVARRRDQLLEA
                     AAEADDDVLTKYLEGEEISDPELEACLRKGVKESILAPVLVGSAVTGIGLQALLDAIV
                     RYLPSPADEPPAKATDKAGTTVELAPDPNGPLLARVFKTAADPFVGRLTYLRILSGTL
                     HSQGHAWNQAHNAEERIGQLLLLHGKEQEPTAELKAGEIGAIAKLAVTGTGDTLSSRE
                     KPFMLAPLAFPEPTLIMAIEPQTKTDLDKMGPALQRMLEEEPSARVERSDAGEQILRT
                     LGDAQAAVIIERLKRKFGAAIVTHTPRVPYRESIRGKASAHGRYKKQTGGHGMFGDVW
                     IEIEPNPGGGVEFAEKVVGGSVPRNFFPGVEKGIRETAAEGVFAGYPLSDFKATLYDG
                     SFHTVDSNELSFKIAASMALKDGILAAKPVLLEPIMTVAIRVPEAYMGEVNRDLNGRR
                     GRVLGMDTKDGMQVINAHVPQSELFTYATELKSITGGRGTFTSTLDRYDEMPSHLADK
                     VVAEHRKETSDSSGH"
     gene            1574..2722
                     /locus_tag="VIF63_00790"
     CDS             1574..2722
                     /locus_tag="VIF63_00790"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8237942.1"
                     /translation="MPQTTPVTFASPVKPAGWTWEATNQEVAARYGLPIEQVVRFDQN
                     TAPSPPGVVQELLAAGHFEVALSEYPPSDYGRLIDAAAARYDVAREELLVGAGADEIL
                     DLVAKTFIPHGGAALVPVPTYPMYAILTEQRGAQVVRVPRRPLESGFALDVEAMRAAV
                     RGANPAINLLWLCNPNNPTATTEPAGVIESLLAGLLSDAEADGRQAPVVLLDEAYVEF
                     GGESELPLRERYPRLVVARTMSKAYAIAGLRVGFAIARPELIDEISTYRPPGSVSIVS
                     VDVAAALLPDGQTMRGRVATVLEERARFAEGLAAAGWEVHPSATNFLLVSFANPAAAD
                     VVAEGLLRRGLIPRTFPSGHPLDHCLRITVRNREQDDRLIEAAREIGA"
     gene            complement(3050..4324)
                     /gene="tyrS"
                     /locus_tag="VIF63_00795"
     CDS             complement(3050..4324)
                     /gene="tyrS"
                     /locus_tag="VIF63_00795"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY8237943.1"
                     /translation="MAGLIPELRWRGMFHAASDGLEARFASNRPITAYNGFDPSGPWL
                     HIGHLVPIFGLIHLQRRGGRPIALVGGGTGMIGDPSGRSSERNLLSRERLAENVASMR
                     PQLERFLDFDGPRGAMMSNNLDWLEPIRLLDFLRDVGKHFTIPYMLAKDSVQIRLEAG
                     LSFTEFSYMLIQATDFQHLYRELDCELQMGGADQWGNITAGLELIRRTSEPRPDGASH
                     AFAMAYPLLLNPSGAKFGKSETGDSVWLHADGTSPYAFYQHWLNTDDRDVPVYLRWFT
                     LFEPARILELEAAMAAAPERREAQRELALNLTTRVHGPDLTRQAVAASEAAFSGASVS
                     DPAVLATLHKDADGFSYTAADTADGVVGFLVATGTSSSKGEARRSIGGGAITVNDRRI
                     TEADPTLPEPIAGEWYVVRIGKRRVRVGRLSR"
     gene            4446..>7107
                     /locus_tag="VIF63_00800"
     CDS             4446..>7107
                     /locus_tag="VIF63_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8237944.1"
                     /translation="MQTALQRRQRHRRNGAARRGRGGGSARRAALAIPLLLFSSFLVL
                     GGVGFVGTVSAYAYYSRDLKDPAALLGELGFEQPSIVYDRTGDIELARFGVLRRELVT
                     FDQIPPEMIDATTAIEDKDYWTNPGFDIGAVVSAGLDTLAGRPRGASTITQQLVRARL
                     LPANAFEGSVYDRKIREIIQSIRLTQAFQGDEGKKKIIEAYLNQNFYGNQSYGVKAAS
                     LGYFGKQLEELTLAQYAVLAAIPQSPTKFDLMQNTLRECAVEVEDGATCPDDKVTLRV
                     PDTSEIVIRRNKVLDFMADPTRSVLSGKDHTAAEYEAAKHEPLILTPQAATQMKAPHF
                     VWQIRKELSSLLCGPENVETCEAVDTGGYQVTTTLDWEMQQTVEKWLFASTRATHLKN
                     PTPVWKGLGIPESEYAWLRNLRGANIHNGAAGIIDYRTGQVLAYGGSGAYYADASGCP
                     KPASDGTTGRCKFEPQFDVMFNGFRQPGSAIKPLNYVIGIENHTLTAATMFMDVVTDF
                     GKKGASYTPSQSDGLERGPVRLRNALQFSFNVPSIKAGIIQGVDLVFNREQEFGLVFP
                     PDAIPVVAQSVGSIEVHPIDLLGAYGAIANSGKLMPRTLILEVKDKDGKVIYPVEGDT
                     KVAKQLVSPQAAYIISDILNGNTTKSINPYWGKWQILEKDKDGTTRRPAAYKTGTTNE
                     RKDTAAYGYLAPPEDPNAPGLAVGIWMGNSDGTPNTDSLSLESSAPLWSRILTEVSQK
                     LPIAKFVRPDGLVDVEVDAFSGLLPGNGTVKTVNEMFIEGTEPTRKDNLHVQTEIDAA
                     TGLLWQEGCTGPKQTRLFLDFSQAEERFPAWQPYTQEWADRAARGAGVRGGPKKTRTM
                     YFYSLGFHPFGATWGGRFKPTEVCQPIVHCP"
BASE COUNT         1185 a         2503 c         2392 g         1027 t
ORIGIN      
        1 gacttcaccg gctatgtcga cctcgtccat cgcaaggcgt tcacctggca gggcggcaag
       61 gacgtcgaga cacagatccc cgacggcatg gccgacgagg tcgcgcgccg ccgcgaccag
      121 ctcctcgagg ccgccgcaga ggccgacgac gacgtcctca cgaagtacct cgagggcgag
      181 gagatatccg accccgagct cgaggcgtgc ctgcgcaagg gggtcaagga gtcgatcctc
      241 gcgcccgtgc tggtcggctc cgcggtgacc ggcatcgggc tgcaggcgct gctcgacgcg
      301 atcgtccgct acctcccctc ccccgccgac gagccgccgg cgaaggcgac cgacaaggcg
      361 ggcacgacgg tcgagctcgc gcccgacccg aacgggccgc tcctcgcgcg cgttttcaag
      421 accgcggccg acccgttcgt cggccggctg acgtacctgc gcatcctctc cggcacgctc
      481 cactcgcagg gccatgcctg gaaccaggcg cacaacgccg aggagcgaat cggccagctg
      541 ctgctgctcc acggcaagga gcaggagccg acggccgagc tcaaggccgg tgagatcggc
      601 gccatcgcga agctcgcggt gaccgggacg ggcgacacgc tctcgagccg ggagaagccg
      661 ttcatgctcg cgccgctcgc cttcccggag ccgacgctga tcatggcgat cgagccccag
      721 accaagaccg acctcgacaa gatgggcccc gcgcttcagc gcatgctcga ggaggagccc
      781 tccgctcggg tggagcggag cgacgcgggc gagcagatcc tgcggacgct cggcgacgcc
      841 caggccgcgg tgatcatcga gcggctcaag cgcaagttcg gcgccgcgat cgtgacgcac
      901 acgccccgcg tgccgtaccg cgagtccatc cggggcaagg caagcgcgca cggtcggtac
      961 aagaagcaga ccggcggcca cgggatgttc ggtgacgtgt ggatcgagat cgagccgaac
     1021 ccgggtggcg gcgtcgagtt cgcggagaag gtcgtcggcg gctcggtgcc gcgcaacttc
     1081 tttcctggtg tcgagaaggg catccgcgag acggccgccg agggcgtctt cgccggctat
     1141 cccctctcgg acttcaaggc gaccctctac gacgggtcgt tccacacggt cgactcgaac
     1201 gagctctcgt tcaagattgc cgcatcgatg gccctcaagg acggcatcct cgcggcaaag
     1261 ccggtcctgc tcgagccgat catgaccgtg gcgatccgcg tgcccgaggc gtacatgggc
     1321 gaggtcaacc gcgacctcaa cggccgtcgc gggcgtgtgc tcggcatgga caccaaggac
     1381 gggatgcagg tcatcaacgc ccacgtcccg cagtccgagc tcttcaccta cgcaacggag
     1441 ctgaagtcga tcaccggcgg tcgcgggacg ttcacctcga cgctcgaccg ctatgacgag
     1501 atgccgtcgc acctcgccga caaggtcgtc gcggagcacc gcaaggagac cagcgactcc
     1561 tcgggccact gacgtgcccc agacgacgcc ggtcaccttc gcctcgccgg tgaagcccgc
     1621 cggctggacg tgggaggcca cgaaccagga ggtcgccgcg cgctacggct tgccgatcga
     1681 gcaggtcgtc cgcttcgacc agaacacggc gccgagcccg ccgggcgtgg tgcaggagct
     1741 ccttgctgcc ggccacttcg aggtcgccct gtccgagtac ccgccgtccg actacggccg
     1801 gctgatcgac gcggccgcgg cccggtacga cgtggcgcgc gaggagctcc tcgtgggcgc
     1861 gggcgcggac gagatcctcg acctcgtcgc caagacgttc atcccccatg gcggcgcggc
     1921 cctcgtcccc gtcccgacct acccgatgta cgcgatcctg acggagcagc gcggcgcaca
     1981 ggtcgtgcgc gtgccgcggc gcccgctgga gtccggcttc gcgttggacg tggaggccat
     2041 gcgcgctgcg gtccgcggcg cgaacccggc gatcaacctc ttgtggctct gcaatccgaa
     2101 caacccgacc gcgacgaccg agcccgcggg cgtcatcgaa tcgctgctcg ccgggctgct
     2161 ctcggatgcc gaggcggacg gtcgccaggc gccggtcgtg ctcctcgacg aggcctacgt
     2221 cgagttcggc ggcgagagcg agctgccgct gcgcgagcgc tacccgcgcc tcgtggtcgc
     2281 ccggacgatg agcaaggcgt acgcgatcgc gggtttgcgg gtcgggttcg cgatcgctcg
     2341 cccggagctc atcgatgaga tctccacgta ccgaccgccg gggtcggtgt cgatcgtctc
     2401 ggtggatgtc gcagcggcgc tcctgccgga tgggcaaacc atgcgtggcc gcgttgccac
     2461 cgttttggag gagcgcgcgc gcttcgccga gggcctcgct gcggccggtt gggaggtcca
     2521 cccctcggcg acgaacttcc tgctcgtgag cttcgcgaac ccggccgccg ccgacgtcgt
     2581 cgccgagggc ctgctccggc gcggcctgat cccgcgcacc ttcccctccg gccacccgct
     2641 cgaccactgc ctgcgcatca ccgtccgaaa ccgcgagcag gacgaccgcc tcatcgaggc
     2701 agccagggag atcggcgcct agggatgcga gccagcctcg gcgcgacgag ccggggcgca
     2761 ccgtcgcagt tcagggcgtc gtcgttgcgc ctgcgatttc tcgatttcgt acggctcgct
     2821 catgcaacgg ttcgtatgcg cgatttgggc cactgattcg atatcacgct cggcgatgcc
     2881 cgtcgccagc atttgaatgg ccccctcgca tgctggcaat acgcatggcc gcgggcgaac
     2941 aattcctcgg gcgggattcc acgcatacga gccgcgggca tggttagcgc ggcggcggct
     3001 ccgtgtggcg ccctggcggc tgagccgccc gatggcgccg tggggcgctc tagcggctga
     3061 ggcgccccac gcgcacgcgg cgcttgccga tgcggaccac gtaccactcg ccggcgattg
     3121 gttcgggcag ggtcgggtcg gcttccgtga tgcggcggtc gttgaccgtg atcgcgccgc
     3181 cgccgatcga gcggcgggcc tcgcccttcg acgatgacgt gccggtcgcc acgaggaacc
     3241 cgaccacgcc atccgcggtg tccgccgccg tgtaggagaa cccatcagcg tccttgtgca
     3301 gcgtcgcgag gacggcaggg tcgctgaccg aggcgcccga gaacgcggcc tcggacgcgg
     3361 ccacggcctg gcgggtcaag tcggggccgt ggacgcgcgt cgtcaggttc agcgccagct
     3421 cgcgctgcgc ctcgcgccgc tcgggtgccg cggccatcgc cgcctccagc tccaggatcc
     3481 gggccggttc gaacagcgtg aaccagcgca gatagacagg cacgtcgcga tcgtccgtgt
     3541 tcagccagtg ctggtagaag gcgtagggcg aggtgccgtc ggcgtggagc catacggaat
     3601 cacccgtctc ggatttgccg aacttcgcgc ccgacgggtt gagcaggagc ggataggcca
     3661 tcgcgaacgc gtgcgacgcg ccgtccgggc gcggctcgga cgtgcgccgg atcagctcca
     3721 gcccggccgt gatgttgccc cactggtcgg cgccgcccat ctggagctcg cagtcgagct
     3781 cccggtacag gtgctggaag tcggtcgcct ggatgagcat gtagctgaac tcggtaaacg
     3841 aaagcccggc ttcgagccgg atctgcaccg agtccttcgc gagcatgtac gggatcgtga
     3901 agtgcttgcc gacatcgcgg aggaagtcca gcagccggat cggctcgagc cagtcgaggt
     3961 tgttcgacat catcgcgcca cgcggcccgt cgaagtcgag gaagcgctcg agctgcggcc
     4021 gcatcgacgc gacgttctcg gcgagccgct cccgtgacag caggttgcgc tcggaggatc
     4081 gcccggacgg gtcgccgatc atgcccgtcc cgccgccgac cagcgcgatc ggccggccgc
     4141 cgcggcgctg gagatggatc agcccgaaga tcgggacgag gtggccgatg tggagccagg
     4201 gcccggaggg atcgaagccg ttgtaggccg tgatcggccg gttcgaggcg aatctcgctt
     4261 cgagcccgtc ggacgcggca tggaacatcc ctcgccagcg cagctccgga atcagccccg
     4321 ccaaaccggc ctcgaacgtg gccaaaggcg tgtcgtccgt cacaggaccg ggtcgagggc
     4381 cgtcgtcagc gcgacccgat ggggggaaag ggccctcacg gaccctatgg tatcgtcgcc
     4441 gccacatgca gacagccctc cagaggcgtc agcgtcaccg ccgcaacggt gccgcgaggc
     4501 gcggacgtgg aggtggctcg gcccgccgcg cggcgctcgc catcccgctc ctcctgttct
     4561 cgagcttcct cgtcctcggc ggcgtgggct tcgtcggcac cgtcagtgcc tacgcgtact
     4621 acagccgtga cctcaaggat cccgccgcgc tccttggcga gctcggcttc gagcagccgt
     4681 cgatcgtcta cgaccgcacc ggcgacatcg agctggccag gttcggcgtc ctccgccgcg
     4741 agctcgtgac cttcgaccag atcccgcccg agatgatcga cgcgacgacg gcgatcgagg
     4801 acaaggacta ctggacgaac cccggcttcg acatcggtgc ggtcgtctcg gccggcctcg
     4861 acacgcttgc cgggcgcccc cgcggcgcgt ccacgatcac ccagcagctc gtccgcgcgc
     4921 gccttctgcc ggcgaacgcc ttcgagggct cggtctacga ccggaagatc cgcgagatca
     4981 tccagtcgat ccggctcacc caggcgttcc agggcgacga aggcaagaag aagatcatcg
     5041 aggcctacct caaccagaac ttctacggca accagagcta cggcgtgaag gctgcgtcgc
     5101 tcggctactt cggcaagcag ctggaggagc tcacgctcgc ccagtacgcg gtcctcgccg
     5161 cgatccccca gtcaccgacg aagttcgacc tcatgcagaa cacgctccgt gagtgcgccg
     5221 tggaggtaga ggatggggcc acctgccccg acgacaaggt caccctccgc gtccccgaca
     5281 cctcggagat cgtcattcgc cggaacaagg tcctcgattt catggccgac ccgacgcgca
     5341 gcgtcctgtc cggcaaggac cacaccgcgg cagagtacga agccgcgaag cacgagccgc
     5401 tgatcctgac cccgcaggcg gcgacgcaga tgaaggcgcc gcacttcgtg tggcagatcc
     5461 gcaaggagct cagctccctg ctgtgcggcc ccgagaacgt ggagacgtgc gaggcggtcg
     5521 acaccggcgg ctaccaggtg acgaccaccc tcgactggga gatgcagcag acggtcgaga
     5581 agtggctgtt cgcctcaact cgcgcgacgc acctcaagaa tccgacgccg gtctggaagg
     5641 gcctcggcat ccccgagtcc gagtacgcct ggctgcgcaa cctgcgcggc gcgaacatcc
     5701 acaacggcgc cgccgggatc atcgactacc gaaccggcca ggtcctcgcg tatggcggca
     5761 gcggcgcgta ctacgcggac gcgtcggggt gcccgaaacc tgcgtcagat ggcacgacag
     5821 gaaggtgcaa gttcgagccc cagttcgacg tgatgttcaa cggcttccgc cagcccggat
     5881 ccgcgatcaa gccgctcaac tatgtcatcg gcatcgagaa ccacaccctc accgcggcaa
     5941 cgatgttcat ggacgtcgtg accgacttcg gcaagaaggg cgcctcctac acgccgtcac
     6001 agtccgacgg gctcgagcgc ggcccggtcc gcctgcgcaa cgccctccag ttctcgttca
     6061 acgtgccgtc gatcaaggcg ggcatcatcc agggcgtcga tcttgtgttc aaccgcgagc
     6121 aggagttcgg gctcgtgttc ccgcccgacg caatcccggt ggtggcccag tccgtgggct
     6181 cgatcgaggt tcacccgatc gacctgctgg gcgcctacgg ggcaatcgcg aacagcggca
     6241 agctgatgcc gcggaccctg atcctcgagg tcaaggacaa ggacggcaag gtcatctacc
     6301 cggtcgaggg cgatacgaag gtagccaagc agctggtcag cccgcaggcg gcctacatca
     6361 tctcggacat cctcaacggg aacaccacca agtcgatcaa cccgtactgg ggcaagtggc
     6421 agatcctgga gaaggacaag gatgggacga cccgacggcc cgcggcctac aagaccggta
     6481 cgaccaacga gcgcaaggac accgcggcgt acggctacct cgcgccgccc gaggacccga
     6541 acgcgcccgg tctcgccgtc gggatctgga tgggcaactc cgacggaacg ccgaacaccg
     6601 acagcctgtc cctcgagtcg tccgccccgc tgtggtcgcg catcctgaca gaggtcagcc
     6661 agaagctgcc gattgcgaag ttcgtgcgac ccgacggcct cgtcgacgtc gaggtcgacg
     6721 cattcagcgg gctcctgccc ggcaacggca ccgtgaagac ggtcaacgag atgttcatcg
     6781 agggcacgga gcccacgcgc aaggacaacc tgcacgtcca gaccgagatc gacgcggcga
     6841 ccggcctgct ctggcaggaa ggctgtaccg gtccgaagca gactcgcctg ttcctcgact
     6901 tcagccaggc cgaggagcgc ttcccggcat ggcagccgta cacgcaggag tgggccgacc
     6961 gcgcggcccg cggcgcgggc gtccgcggcg gtcccaagaa gacccggacg atgtacttct
     7021 acagcctggg cttccacccc ttcggggcga cgtggggcgg ccggttcaag ccgaccgagg
     7081 tctgccagcc gatcgtccac tgcccgc
//
LOCUS       DATBSW010000027         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1130377, whole genome shotgun sequence.
ACCESSION   DATBSW010000027 DATBSW010000000
VERSION     DATBSW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130377"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VIF63_00805"
     CDS             complement(<1..1305)
                     /locus_tag="VIF63_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8237945.1"
                     /translation="MQLPDRPPFVLRARVLTPLASGGVADHPDGVVSVDANGRIDWVG
                     DALEWAARLPASGPHQDPGPDREDDRAEPIDLRPFVLLPGMVDLHAHLPQLPNAGLGA
                     GLDLLTWLDRYIFPMERRFDAAEDAARLAPAAFQTFAAAGTTTLLAYGAVYKASMDGA
                     FRAAEEHGIRAILGKVMMDRVTYDETIDPSTILERSVRESEELIADWHGADEGRLGYA
                     VTPRFAVSCTAELLRESAALAERTGTWWQTHVSEDPGEIQEVARLFPEARDYVDVYDR
                     AGGLGARTILAHAIHLSDRELTRLVDTDTRVAHCPSSNLFLASGIMPLARYREAGLRI
                     GLGSDVAGGPDPSIFSVMQVGAYAQAALRTVAGESRPSLAPLDWLRAGTLDGARVLGL
                     DDRIGSLEAGKEADLIAIDPRLTAPRRDLPYDARRVEADEVMS"
     gene            complement(1316..2251)
                     /locus_tag="VIF63_00810"
     CDS             complement(1316..2251)
                     /locus_tag="VIF63_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8237946.1"
                     /translation="MSLPRASSLLALAVILAACGGTTTSTSLPVITPGATAVPATQAP
                     QATAPPVATPASTAAPAPAINGERVSFKNGELTLGGLLWKPEGNGPFRTVVWNHGSEQ
                     LPGSPQGNPLVGTALVAQGYVVFVPFRRGQGTSQGTWIDTALKAVPVAERPALLAKLH
                     TTEQLSDQLAGLAYIKSQAYVDPTKIAVAGWSFGGIQTVLGAGDASAGYVAAVAFTPA
                     SQSWDGNPDLQAALLTAAKAATVPFLFIQAENDYSLVPTQELTDAIVADEGTASRSIY
                     PAFGSTAQDGHEFAVRGTDQWIPEVVTFLNAAFGA"
     gene            2321..>2565
                     /locus_tag="VIF63_00815"
     CDS             2321..>2565
                     /locus_tag="VIF63_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237947.1"
                     /translation="MTSDERRYELLILDGTVVDGSGAAGFAAAVGVTRGHAGASARIV
                     VLRDAAAIEDAIGRAARLVRAAGKVVAPGFIDLHSHSG"
BASE COUNT          364 a          858 c          984 g          359 t
ORIGIN      
        1 gctcatgacc tcgtcggctt cgacgcgtcg ggcgtcgtag ggcaggtcgc gtcgcggggc
       61 ggtcaggcgc gggtcgatcg cgatcaggtc ggcctccttg cccgcctcga gcgagccgat
      121 ccggtcatcg agaccgagca cccgggcgcc gtcgagcgtg ccggcgcgca gccagtccag
      181 cggggcgagt gatggacggc tctcgccggc cacggtccgc agcgccgcct gtgcgtatgc
      241 gccgacctgc atgacggaga agatcgaggg gtcggggccg cccgcgacgt cggagcccag
      301 gccgatccgg agaccggcct cacggtagcg ggcgaggggc atgatcccgg aggccagaaa
      361 caggttcgac gaggggcaat gggcgacgcg cgtgtccgta tcgacgagac gcgtgagctc
      421 gcggtccgag aggtgaatcg cgtgggcgag gatcgtccgc gcaccgaggc cgccggcgcg
      481 gtcgtagacg tcgacgtagt ccctcgcctc gggaaagagg cgcgcgactt cctggatctc
      541 gcccgggtcc tccgagacat gggtctgcca ccacgtcccg gtccgttccg cgagcgccgc
      601 cgactcgcgc agcagctccg cggtgcacga gaccgcgaac cgcggcgtga ccgcgtagcc
      661 cagccggcct tcgtcggcgc cgtgccagtc ggcgatgagc tcctcggatt cgcgaacgga
      721 gcgctcgagg atcgtcgacg gatcgatcgt ctcgtcgtac gtgacccgat ccatcatcac
      781 cttgcccagg atcgcccgaa tgccgtgctc ctcggccgcg cggaacgcgc cgtccatcga
      841 tgccttgtag accgcgccgt aggcgagcag cgtggtggtg ccggcggccg cgaaggtctg
      901 gaacgcggcg ggggcgaggc gagcggcgtc ctccgcagca tcgaaccggc gttccatcgg
      961 gaagatgtat cggtcgagcc aggtgagcag gtcgaggccg gcgccgaggc cggcgttcgg
     1021 gagctggggc aggtgcgcgt gcaggtcgac catgcccggc aggagcacga acggccgaag
     1081 gtcgatcggt tcggcgcggt cgtcctcgcg atcgggtccc ggatcctggt ggggccctga
     1141 cgccggcagc cgagctgccc actcgagcgc gtcgcccacc caatcgatcc gtccgttcgc
     1201 atccaccgag acgacgccat cgggatgatc ggcgacgcca ccgctcgcga gcggggtcag
     1261 gacgcgggcg cgcagcacga atggcggtcg atccgggagc tgcatcgggc gagggctacg
     1321 cgccgaacgc ggcgttcagg aaggtgacga cctcggggat ccactgatcg gtgccgcgca
     1381 cggcgaactc gtgcccgtcc tgggcggtcg agccgaaggc cgggtagatc gagcggctgg
     1441 ccgtgccctc gtccgcgacg atcgcgtcgg tgagctcctg ggtcggcacg aggctgtagt
     1501 cgttctcggc ctggatgaac aggaatggca cggtcgcggc cttcgccgcc gtgagcagcg
     1561 ccgcctggag gtcgggattg ccgtcccagg actgcgacgc aggcgtgaac gccacggcgg
     1621 cgacgtagcc ggccgaggcg tcgccggcgc cgaggacggt ctggatgccg ccgaacgacc
     1681 aacctgcaac ggcgatcttc gtcgggtcga cgtacgcctg gctcttgatg taggcgagcc
     1741 cagcgagctg gtcgctgagc tgctcggtcg tgtgcagctt ggcgagcagc gccggtcgct
     1801 cggcgacggg cacggccttg agcgccgtgt cgatccacgt cccctgcgac gtgccctgcc
     1861 cgcgccggaa tgggacgaac acgacgtagc cctgcgcgac cagcgccgtt ccgacgagcg
     1921 ggttgccctg cggcgagccg gggagctgtt cgctgccgtg attccagacg accgtccgga
     1981 acgggccgtt gccctccggc ttccagagca ggccgccgag cgtgagctcc ccgttcttga
     2041 aggacactcg ctcgccgttg atggcaggcg caggcgctgc cgtcgatgcc ggcgttgcaa
     2101 ccggcggagc ggtggcctgt ggtgcctgcg tcgcgggcac ggcagtcgca cccggggtga
     2161 tgaccggcag ggacgtggac gtcgtcgtgc cgccgcacgc ggcgaggatg accgcgagag
     2221 cgaggaggct cgaggcgcgg ggcagcgaca tgggttcggc gtctcctcag aggtcgtcgc
     2281 ggagcatacg acgcgacacg cggctggcac catgagcgcg atgacctccg acgagcgccg
     2341 gtacgagctg ctgatcctcg atggcaccgt cgtcgatggg agcggcgccg ccgggttcgc
     2401 ggccgcggtc ggcgtcaccc gcgggcacgc cggggcgagc gcgcggatcg tggtcctgcg
     2461 ggatgcggct gcgatcgagg acgcgatcgg gcgagcggcg cggctcgtgc gcgccgcggg
     2521 caaggtcgtc gcgccggggt tcatcgacct gcacagccac tcggg
//
LOCUS       DATBSW010000028         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1201244, whole genome shotgun sequence.
ACCESSION   DATBSW010000028 DATBSW010000000
VERSION     DATBSW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201244"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="VIF63_00820"
     CDS             <1..1830
                     /locus_tag="VIF63_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8237948.1"
                     /translation="RNKEPDIALVVVIDKSGSMDACHCNTFDGGMGGGGAMSGVRKVD
                     IGKEAILRAAAALTSRDEFGVVAFDQSAHWVVQTQPLGGITDLQGSVASINPLGQTNI
                     FSGLDQAVGSLEKATATRRHIILLTDGWSTSGQYDDIIKRMKAAGITLSTVGAGGGSN
                     PFLEQLARNGGGRFYAAANVASIPDIFLKETQQVAGQQIVEEKFFPIQAGNSPIIRGL
                     EAGFPQLLGYNGTTIKSAAQLVLVSPRDDPLLAQWQYGLGRSVAWTSDSTGRWAKNWV
                     GWQGFTRFFSQLVAWTFPGEETGGIEASFESLGGKTRLHIESVESDGSPRDFYRTMAT
                     IVGPDLEPHEVLLDQVAPGVYEQDLGEIDSGAYAVRITQTRPGAAALGRTVGLVEPVA
                     AEYRLLGVNLPLLAALRAATGGREIDLPTDPWKHDLGLTSSFTELWPWLLILALLLWP
                     LDIALRRVSIGRRELVDARAWVGRRWRSRGAAAPRTVASEGMLASRERAAGGAARAAL
                     LRGPDTTAAPVDSAPSTDATKPRARTSPPQRGTTAGPASAPAPAVPEIKAPAATSQPG
                     PAAAPSPKPGAKPAAKPADSPPLEEMDTLARLRESKQRRSRGG"
     gene            1866..>3390
                     /locus_tag="VIF63_00825"
     CDS             1866..>3390
                     /locus_tag="VIF63_00825"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY8237949.1"
                     /translation="MSSRPIGTDSPSVPRVLWAPPADILDRSRMGAYLAWLHEARGLQ
                     FEAYDALWQWSVDEPGAFWRSVWDFFEVIAHAEPTADLADARMPGARWFPGATLNYAE
                     HALRIPGRSGSDVAVVGRSQTRHPSELTADELRDAVARCRAGLVRLGVGRGDRVAAYF
                     PNIPEAIVALLATASLGATWSSCAPEFGARAVIDRFGQIEPKVLLSIDGYRYGDRDVD
                     RRAELAEIRTALPSLDATVVLPYLDPDAVGSIEGTIAWDELLAESGPLAFEPVPFDHP
                     LYILYSSGTTGLPKPIVHGHGGITLEHLKALALHTDLGPGDRFCWFTTTGWMMWNYLV
                     SGLLVGSTVVTFDGNPAFPDLAGLWRVAAETHATYLGTSAPYLMACRKAGLTPGRDLD
                     LSALRGVGSTGAPLPASGFEWVFDAVSSAVPVGSLSGGTDMCTGFLGPSPLVPVWSGE
                     ISCRMLGARVEAFDEGGRSVVGREGELVITAPMPSMPVGLWGDDDGSRLREAYFSIYP
                     "
BASE COUNT          460 a         1222 c         1194 g          514 t
ORIGIN      
        1 cggaacaagg agccggacat cgccctcgtc gtcgtcatcg acaagtcggg ctcgatggac
       61 gcctgccact gcaacacctt cgacggcggc atgggcggcg gtggcgcgat gtccggcgtg
      121 cgcaaggtcg acatcggcaa ggaggcgatc ctgcgcgccg cggcggcgct gacctcgcgc
      181 gacgagttcg gcgtggtggc gttcgaccag tccgcgcact gggtcgtcca gacccagcca
      241 ctcggtggta tcaccgacct ccagggctcg gtcgcgagca tcaacccgct cggccagacg
      301 aacatcttct ccggcctcga ccaggccgtc ggctcgctcg agaaggcgac ggcgacccgg
      361 cgccacatca tcctcctgac cgacggctgg tccacgtccg gccagtacga cgacatcatc
      421 aagcgcatga aggccgccgg gatcacgctc tcgacggtcg gggcgggcgg tggctcgaac
      481 ccgttcctcg agcagctcgc ccggaacggt ggcggccggt tctatgcggc cgcgaacgtc
      541 gcctcgatcc ccgacatctt tctcaaggag acccagcagg tcgccgggca acagatcgtg
      601 gaagagaagt tcttcccgat ccaggccggc aactcgccga tcatccgcgg cctcgaggcc
      661 ggcttcccgc agctcctcgg ctacaacggc acgacgatca agtcggccgc gcagctcgtc
      721 ctcgtctccc cgcgcgacga tcccctcctc gcccagtggc agtacggcct gggccggtcc
      781 gtcgcgtgga cctcggactc gaccgggcgc tgggccaaga actgggtcgg ctggcagggc
      841 ttcacgcgat tcttcagcca gctcgtcgcc tggacgttcc cgggtgagga gacgggcggc
      901 atcgaggcgt cattcgagtc gctcgggggc aagacccggc tgcacatcga gagcgtcgaa
      961 tcggacgggt cgccgcgcga cttctaccgg acgatggcga cgatcgtcgg gccggacctc
     1021 gaaccacacg aggtgctcct cgaccaggtc gcgcccggcg tgtacgagca ggacctcggc
     1081 gagatcgatt ccggcgccta cgccgtgcgg atcacacaga cgcgtccggg cgccgcggca
     1141 ctcggacgga cggttgggct ggtcgagccg gtcgcggcgg agtaccggct gctcggcgtc
     1201 aacctgccat tgctggcggc cttgcgcgct gcaaccggcg gtcgcgagat cgacctgccg
     1261 accgatccgt ggaagcacga ccttgggctg acctcgtcgt tcacggagct gtggccgtgg
     1321 ctcctgatcc tggcgctgct cctctggcca ctggacatcg cgctccggcg ggtgtcgatc
     1381 gggcgacgcg agctcgtgga tgcgcgcgcc tgggtgggcc ggcgctggcg gtcgcggggg
     1441 gccgccgcgc cgcggacggt cgcctcggag ggcatgcttg cgtcacggga gcgcgccgcc
     1501 ggtggcgccg ctcgtgccgc gctgttgcgc gggccggaca ccactgcggc accggtcgat
     1561 tcggcgccat ccacggacgc cacgaagccg cgggccagga cgtcgccgcc gcagcggggc
     1621 accaccgccg gaccggcgtc cgcgcccgcg ccggccgtcc cggagatcaa ggcccccgcg
     1681 gcgacgagcc agccggggcc cgccgccgct ccctcgccga agcctggcgc caaacccgca
     1741 gccaagcccg cggactcccc gccgctcgag gagatggata cgctggcccg gctgcgcgag
     1801 tccaagcagc gaaggtcgcg cggcggctga cgtccgccca cagtcacctg cgctaccgtg
     1861 ctgcagtgag cagtaggccg atcgggaccg actcgccgag cgtgccgcgc gtcctatggg
     1921 cgccacccgc cgacatcctc gaccggtcgc gcatgggcgc gtacctggcg tggctgcacg
     1981 aggcacgcgg cctccagttc gaggcctacg atgcgctctg gcagtggtcg gtcgacgagc
     2041 cgggcgcctt ctggcgatca gtctgggact tcttcgaggt catcgcccac gccgagccca
     2101 ccgccgacct tgccgacgcc cggatgcccg gcgcgcgctg gttccccggc gcgacgctca
     2161 actacgccga acatgcgctg cgaattccgg gtcggtcggg ctccgacgtg gcggtcgtcg
     2221 ggcgatcgca gacgcggcat ccgagcgagc tgacggccga cgagctgcgc gacgccgtcg
     2281 cccgatgccg ggccggactc gtgcgactcg gcgtgggccg gggcgatcgc gtggccgcgt
     2341 actttccgaa cattccggag gcgatcgtcg cgctgctggc aaccgcgagc ctcggtgcga
     2401 cgtggtcgtc ctgcgcgccg gagttcggtg cccgcgcggt catcgatcga ttcggccaga
     2461 tcgagccgaa ggtgctgctg tcgatcgatg gctatcgcta cggcgaccgc gatgtcgacc
     2521 gtcgcgccga gctcgccgag atccgcacag cgctgccgtc actcgatgcg accgtcgtgc
     2581 tcccctacct cgacccggac gccgtcggct cgatcgaggg cacgatcgcc tgggacgagc
     2641 tgctggccga gtccggcccg ctggccttcg agcccgtacc gttcgatcac ccgctgtaca
     2701 tcctctattc gtctggcacc acggggttgc ccaagccgat cgtgcacggc catggcggga
     2761 tcaccctcga gcacctcaag gcgctcgcgc tgcacaccga cctcgggccg ggcgatcgct
     2821 tctgctggtt cacgacgacc ggctggatga tgtggaacta cctggtgtcg gggctgctcg
     2881 tcgggtcgac cgtcgtcacc ttcgacggca acccggcgtt ccccgacctc gccgggctgt
     2941 ggcgcgtcgc cgccgagacc cacgcgacgt atctcgggac cagcgcgccg tacctcatgg
     3001 cctgccgcaa ggccgggctg acacccgggc gcgacctcga cctgtcggcg ctgcgcggcg
     3061 tcggttcgac cggcgcgccg cttccggcct ccggcttcga atgggtcttc gatgccgttt
     3121 cgagtgccgt gcccgtcggg tccctgagcg gcggtacgga catgtgcacc ggattcctgg
     3181 ggccatcgcc gctggtgccg gtctggtccg gcgagatcag ctgccggatg ctcggggctc
     3241 gggtggaggc gttcgacgaa ggcggccggt ccgtggtcgg gcgggagggc gagctggtca
     3301 tcacggcgcc gatgccgtcg atgccagtgg gcctgtgggg cgacgacgac gggtcgcgcc
     3361 tgcgcgaggc gtacttctcg atctatcccg
//
LOCUS       DATBSW010000029         9562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_284302, whole genome shotgun sequence.
ACCESSION   DATBSW010000029 DATBSW010000000
VERSION     DATBSW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284302"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VIF63_00830"
     CDS             <1..507
                     /locus_tag="VIF63_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237950.1"
                     /translation="ATYPGVWRHGDWLTITERGSCIVTGRSDATLKRGGVRIGTAEFY
                     GIVEALPDVADSLVVHLEDATGGGPGELLLFVVPRPGVVLDGALRGRIATALREELSP
                     RHVPDQILAVPAVPRTLSGKKLEVPVKRILLGAAPDATASKEALVDPGALAPFEALAR
                     ERLAADTR"
     gene            528..983
                     /locus_tag="VIF63_00835"
     CDS             528..983
                     /locus_tag="VIF63_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237951.1"
                     /translation="MRHSPALSFVLVALVVAGCAGLLESPPVPDPAPFPEISGQLGRV
                     GVDVESWTAGDAGCDDPSLNPTAIRFTASGLDQSTPVQLRIYIFRNRDAWERRLADVD
                     TCAASWAEDPATFELVQISPYVLAGQGPWPPQFDAAIREGLTAAAGTGG"
     gene            1204..1971
                     /locus_tag="VIF63_00840"
     CDS             1204..1971
                     /locus_tag="VIF63_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY8237952.1"
                     /translation="MTRLTRRSQRASSTALILLLLAITTGWTQAATPTVHDGRPADGA
                     PLREAAALPLAALNASNLMAYRESEAALAITGRALYQAHVVEMRAAPPAPVRVETPVS
                     KTPVVKAKVVKAPAVKTPAVKAAPTYSGTSHFWFPALGISKSVHMFECTRSRAPDNYV
                     YRWGCAGKNNVYLLGHAASVFRSLHDAYLSGRLRVGMVAIYADSKGRITKFKVTSWRV
                     VKPENAAWAIASQPVPSMTLQTCIGLKRLNVRLVAIN"
     gene            complement(2026..3213)
                     /locus_tag="VIF63_00845"
     CDS             complement(2026..3213)
                     /locus_tag="VIF63_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8237953.1"
                     /translation="MDLDLSAEHQLLRSTIRDFMENEVAPVIEEHEREHKFPVEVVRR
                     LGEMGWLGIPIPEDEGGAGMDTLAYAIAIEEIGRVWGSLGLIVAAHTSLGCGPLHLAG
                     TKEQKDRYLVPMASGNVIGAYGLTEPGAGSDAGGTRTTARWEGADGNDFVPGGQGEWV
                     VDGSKRFITNAGQAGTYIITAKTGAKDDGNAEISAFILPADTPGFRVVRIEDKLGLHA
                     SATGELAFDRCRIPAENLLGERGDGFRTFLKILDGGRISIAALAVGIAQAAFDAAVPY
                     AQTRQQFGKPIGSFQGVAFMIADMATEIEAARALVWKSAWLKDQGRDYSLVAAEAKLF
                     ASEVSSRVTNAAIQVHGGYGYVTEYPVERFMRDAKLTEIGEGTSQVQRLVIARKILGL
                     RIG"
     gene            3297..4001
                     /locus_tag="VIF63_00850"
     CDS             3297..4001
                     /locus_tag="VIF63_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237954.1"
                     /translation="MAQPVGAVSQARAGNGGPSPSALRHEILVHLRQAGPSSPDTVAG
                     ALHASRTGVLQQLYALEDAGLVSHAAEKHGVGRPRHLYDVTPDAQGLFPTDYGGFASG
                     LVKAIEAVGGADLVEQVFAARQRQIGDRIQRRMGERLPDDASVADRVRVLATLQDEAG
                     YLAEAIIGDDGSMRLREHNCAISKISKRTTAPCDAELALFKDVLGANVERESHIASGD
                     RCCTYVVSQRQPDRAD"
     gene            complement(3998..5053)
                     /locus_tag="VIF63_00855"
     CDS             complement(3998..5053)
                     /locus_tag="VIF63_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237955.1"
                     /translation="MVRQLPRTVPLAELHCHLGAAVTPAIMWGIAHSQGIKLPTKDYW
                     EFRDLITVPAKGSKSFDGYLQLFHWTELIQSSPLAVERSVYEVIGGAYRKNNITTMEL
                     RFNPMKRNRGGEQDLDHIIAAAARGMDKASLEYPVRPGLIFCLDRAFPFELNEIIAEK
                     AITWRDRGVVGVDIAGPESATFRVADYRKLMRRVRQFGLGITVHTGESGPVEEIARVV
                     ELLEPDRIGHGVKAAYDPRAMKMISERGIVLEICPTSNLNTRVVSGWDEVRWILDTFR
                     RNDVRFIISTDGPEMLQTYIRDELATLGKLGILSVEEQAQVAATAMAASFVPNVSDVP
                     FTQRRPATREVVEREEA"
     gene            5172..5903
                     /locus_tag="VIF63_00860"
     CDS             5172..5903
                     /locus_tag="VIF63_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEY8237956.1"
                     /translation="MQISRRLSRRRLATAAFLVVGLLAFPLGVIASHQYSDVPDSNPY
                     HADIDAITDAGVTTGCGNGSMYCPSAFVTREQMAAFMNRLGALQAGKTPVVNATKVDG
                     FNANDLHRVAAYVVPTLIDSSAISTTGTLAGTITIPQKGYLIVAANTTLYTPGTAGST
                     TCQLQFSGGSAWPGTYHQTTTGTPMVVNQCTTQAVIEVCNPGSPYTVEFYVSAGTGDT
                     SAQNATLIIQYVPFDGEGLRPDCLG"
     gene            complement(5918..6895)
                     /gene="lipA"
                     /locus_tag="VIF63_00865"
     CDS             complement(5918..6895)
                     /gene="lipA"
                     /locus_tag="VIF63_00865"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY8237957.1"
                     /translation="MGGGAFGAAADGSAALRRHPEWIKARLPSGENYHDLKRLLTGLN
                     LNTVCEEAHCPNIGECWDQRTATIMILGDTCTRACGFCNIKTGKPTWFDDDEPRRVAE
                     AIQQLGLEHVVVTSVARDDLPDGGAHVFADTIRLLHEQVPGMGVEVLIPDFNGDEAPL
                     RDVMAARPDILNHNLETVRRLQKPVRKRARWDRSLGVLERAKAYAAEAPYAVHTKSSL
                     MVGLGETRDELTEAFEALRDVDTDILTIGQYLRPSLKHLPLERYYHPDEFAEMKTEAL
                     GLGFKHVESGPLVRSSYHARDQVPGAALKTLRRRRATLDADGRVVPIAG"
     gene            complement(7011..8564)
                     /locus_tag="VIF63_00870"
     CDS             complement(7011..8564)
                     /locus_tag="VIF63_00870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY8237958.1"
                     /translation="MAKVTMPQLGESVAEGTIGKWLKQPGDHVAKYEPLLEVITDKVN
                     AEVPSPYEGILKEILVEEGATVPNNAEIAIIEEAGAAASTAASTAAPAATAATASTSA
                     APSNGSGAAPKESAALAPAASAPTPAPASAAAPAPEAPASAAVASPMASAAPAVSQPS
                     NGPAGSDERGVHELPGSADARMTPAVRRLLREHGLSAAMIVGTGGGGRITREDVTDYV
                     ESQRTSKPVGRQDASATPAASAPAGAAAPAAPAAATSAGPAPAAAPRAAAPATQSSAT
                     IAFPEGADEVLVPMTQMRKGIAAQMNRALQVPHAYVQMEVDVSSLVRFRDREKKGYQA
                     REGISLSFVPFVVKASAEALKRNPTFNAHWTENGLLAKRRINIGIAVAVEGGLIVPVI
                     RDVDNLSISGLNKAIAEVSDRARANKFQLDDFGGGTFTVDNTGWLGTNMVMPIINVPE
                     VGIVTMDRITKRPVVVETPEGDVIGIRSIMNMVLGVDHRANDGAGGAALLRDIKAWLE
                     AVGPDTAIY"
     gene            8716..>9562
                     /locus_tag="VIF63_00875"
     CDS             8716..>9562
                     /locus_tag="VIF63_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEY8237959.1"
                     /translation="MLNLLVILAGLVILGASLLFGIFLPLSRPEPRGSDPRFAQQPRR
                     LHLLNGIVGATIGGIILVIGFAGPFVEVPAGNVGVVTNFGSVQRVTLEPGLHVVIPIV
                     QHVTNVDTRVQPHHFQEIDAASKELQTVRLTGTMNYHIDGQFANDLFQRVGTDFAEKV
                     IDPAFNDFIKTVVPGYSVNEILAKRDEIRSLAKDLLGANLAQYHIIVDDIYIANIAFS
                     DGFQQAIEDKQVAEQRVQTEQQILAQRQIQAQQAVAQAKGQADSIFTLAEGQSRANAL
                     LAASLT"
BASE COUNT         1397 a         3311 c         3302 g         1552 t
ORIGIN      
        1 gcgacgtatc ccggcgtgtg gcgccacggc gactggctca cgatcaccga gcgcgggtcg
       61 tgcatcgtga cgggccgcag cgacgcgacg ctgaagcgtg gtggcgtgcg gatcgggacg
      121 gccgagttct acggcatcgt cgaggcgctg ccggacgtcg ccgacagcct cgtcgtgcat
      181 ctcgaggacg cgaccggcgg cgggcccggt gagctgctgc tgttcgtggt tccgcggccg
      241 ggggttgtcc tggatggggc gctgcgcggg cggatcgcga cggcattgcg cgaggagctt
      301 tcgccgcgcc acgtcccgga ccagatcctg gccgtgccgg ccgtgccgcg gacgctgtcc
      361 ggcaagaagc tcgaggtgcc ggtcaagcgg atcctgctcg gtgcggcgcc ggatgcaacg
      421 gcatcgaagg aggcgctcgt cgatcccggc gcattggcgc ccttcgaggc cctggcgcgc
      481 gagcggcttg cggcggatac tcgctgatgc cggcacgctc gagcgcaatg cggcactccc
      541 ccgccctttc cttcgtcctg gtcgcgctcg tcgtcgcggg ctgcgcgggc ctgctcgagt
      601 cgccgccggt cccggacccg gcgccgttcc ccgagatttc ggggcagctt ggccgcgtcg
      661 gcgtcgatgt cgaaagctgg accgccggcg acgcgggctg cgacgaccct tcgctcaatc
      721 cgaccgcgat ccgcttcacc gcgagcggcc tcgaccagtc gacgcccgtc cagctgcgga
      781 tctacatctt ccgcaatcgc gatgcgtggg agcgtcggct cgctgatgtc gacacctgcg
      841 cggcgtcgtg ggccgaggac ccggccacgt tcgagctggt gcagatctcg ccgtacgtgc
      901 tcgccggtca gggaccgtgg ccgccccagt tcgacgcggc catccgggaa gggctcaccg
      961 cggcggccgg caccggcggc tgagcctccg cgcggccgtc ctggggcgcg ccagttgcgt
     1021 ccctgcacca cggcggatgc gccgtccccg ttgcatccac gcaacgttgc atataccggc
     1081 ctgaccagat cgtcccgtcc ggtcccggtc atggtccgat gacccggacc gtccctctgc
     1141 gctacggcct gcgggaccag cgggtggggg tcgcgggacg cctccccggg cgaccgttgg
     1201 gccatgacca gattgacacg gcgcagtcag cgcgcgtcct ccacagcgct gattctcctg
     1261 ctcctcgcga tcaccaccgg atggacccag gcggcgacac caactgtcca cgatggacgg
     1321 ccggccgacg gcgcaccgct gcgggaagcg gccgcgctgc cgctcgcggc gctcaacgca
     1381 tccaatctca tggcgtatcg cgaatccgag gcggccctcg cgatcaccgg ccgagccctg
     1441 taccaggccc atgtggtcga gatgcgagcg gcaccgcccg ctccggtgcg ggtcgagacg
     1501 cccgtgtcca agacgccggt ggtcaaggcc aaggtcgtca aggcgcccgc ggtcaagacg
     1561 cccgcggtca aggcggcgcc gacgtattca gggaccagcc acttctggtt ccccgcactc
     1621 gggatctcga agtcagtgca catgttcgag tgcacccgca gtcgcgcgcc agacaactac
     1681 gtgtaccgct ggggctgcgc cggcaagaac aacgtctacc tcctgggaca cgcggcgtcg
     1741 gtcttccggt cgctccacga cgcgtacctc tcgggtcgcc tgcgtgtcgg gatggtggcg
     1801 atctacgccg acagcaaggg ccggatcacg aagttcaagg tcacctcgtg gcgggtcgtg
     1861 aaaccggaga atgcggcctg ggccatcgcg agccagccgg tcccgagcat gaccctgcag
     1921 acgtgcatcg ggctcaagcg cctgaacgtg cggctggtcg cgatcaactg aggtcgcgcg
     1981 tcgcgggctg gccggcgcgc ggcgggctag cggacacgtc gcgggctagc cgatccggag
     2041 gcccaggatc ttccgggcga tcacgagccg ctgcacctgg ctcgtcccct cgccgatctc
     2101 cgtcagcttg gcgtcgcgca tgaaccgctc gaccgggtac tcggtcacgt agccgtagcc
     2161 gccgtggacc tggatcgccg cattcgtgac ccgtgagctg acttccgacg cgaacagctt
     2221 cgcctccgcc gcgaccagcg agtagtcgcg accctggtcc ttgagccagg ccgacttcca
     2281 gaccagcgcc cgggccgcct cgatctcggt cgccatgtcg gcgatcatga acgccacccc
     2341 ctggaacgag ccgatcggct tgccgaactg ttgccgcgtc tgggcgtacg gcaccgcggc
     2401 gtcgaacgcc gcctgcgcga tccccaccgc cagcgcagcg atcgagatcc gcccgccgtc
     2461 gaggatcttc aggaacgtcc ggaagccgtc gccgcgctcg ccgagcaggt tctcggccgg
     2521 gatccggcag cggtcgaacg cgagctctcc ggttgccgac gcatgcagtc cgagcttgtc
     2581 ctcgatccgg acgacgcgga acccgggtgt gtcggccggc aggatgaacg ccgagatctc
     2641 ggcgttgccg tcgtccttcg ccccggtctt ggcggtgatg atgtaggtcc ctgcctggcc
     2701 ggcattggtg atgaaccgct tgctgccgtc gaccacccac tcgccctgcc cgccgggcac
     2761 aaaatcgttc ccgtcggcgc cctcccagcg cgcggtcgtg cgcgtgccgc ctgcatcgga
     2821 gccggcgccc ggctcggtga gcccgtatgc accgatgacg ttgccgctcg ccatcgggac
     2881 caggtagcgg tccttctgct ccttcgtgcc ggcgaggtgg agcgggccgc agccgagcga
     2941 ggtgtgcgcg gccacgatca gcccgagcga gccccagacc cggccgatct cctcgatcgc
     3001 gatcgcgtag gcgagcgtgt ccatcccggc gccgccctcg tcctccggga tcgggattcc
     3061 gagccagccc atctcgccga gtcggcggac gacctcgacc gggaatttgt gctcgcgctc
     3121 gtgctcctcg atgaccggcg cgacctcgtt ttccatgaag tcgcggatgg tcgagcggag
     3181 gagctgatgc tccgcggaga ggtccaggtc catcgcggcg gagcctagca tcgggcgccg
     3241 aacgggcgat tcggggccgt cctccggatg gtggtttttc tgctagcgtc ccgtgaatgg
     3301 cacagcccgt cggggcggtt tcgcaagccc gagccggcaa tggcgggcca tctccatccg
     3361 cgctacgaca cgagatcctc gtgcacctgc gccaggcggg cccgtcctcc cccgacacgg
     3421 tcgccggagc gctgcatgcc agtcggacgg gcgtcctcca acagctgtac gcgctcgagg
     3481 acgccgggct cgtcagccac gccgccgaga agcacggcgt cggccggcct cgtcacctct
     3541 acgacgtcac gccggatgcg cagggcctct tcccgaccga ctacggcggg ttcgcctcgg
     3601 gcctggtgaa ggcgatcgag gcagtcggcg gcgccgatct cgtcgagcag gtcttcgccg
     3661 cgcgccagcg ccagatcggc gatcggatcc agcggcgcat gggcgagcgg ctgcccgacg
     3721 acgccagcgt ggcggatcgc gttcgcgtcc tggccacgct ccaggacgag gccggctacc
     3781 tcgccgaggc gatcatcggc gacgacggct cgatgcggct gcgggagcac aactgcgcga
     3841 tcagcaagat ctcgaagcga acgacggcgc cgtgcgatgc ggagctggca ctgttcaagg
     3901 acgtcctcgg cgccaatgtc gagcgcgaat cgcacatcgc atcgggcgac cggtgctgca
     3961 cctacgtcgt gagccagcgg cagcccgacc gagcggacta ggcctcttcc cgctccacga
     4021 cctcgcgcgt ggcgggccgg cgctgggtga acgggacgtc cgagacgttc gggacgaacg
     4081 aggcagccat cgcggtcgcc gcgacctgcg cctgctcctc gaccgacagg atgccgagct
     4141 tgccgagggt cgccagctcg tcgcggatgt acgtctggag catctcgggg ccgtccgtgc
     4201 tgatgatgaa gcggacgtcg ttgcgccgga acgtatcgag gatccagcgg acctcgtccc
     4261 agcccgagac gacgcgcgtg ttcaggttcg atgtcgggca gatctcgagg acgatgccgc
     4321 gctcgctgat catcttcatc gcccgcgggt catacgcggc tttcacgccg tgcccgatcc
     4381 ggtcgggctc gagcagctcg acgacgcgcg caatctcctc gaccgggcct gactcgccgg
     4441 tgtgcaccgt gatgccgagg ccgaactggc ggacgcggcg catgagcttg cggtaatcgg
     4501 cgacgcggaa ggtcgcggat tccgggccgg cgatgtcgac gccgacgacc cctcgatccc
     4561 gccaggtgat cgccttctcc gcgatgatct cgttgagctc gaacgggaat gcgcggtcga
     4621 ggcagaagat caggccgggc cgcaccgggt attcgaggct cgccttgtcc atgccccggg
     4681 cggcggcggc gatgatgtgg tcgaggtcct gctcgccgcc gcggttccgc ttcatcgggt
     4741 tgaagcggag ctccatggtc gtgatgttgt tcttgcggta cgccccgccg atgacctcgt
     4801 agacgcttcg ctcgacggcc agcggcgagc tctggatcag ctcggtccag tggaagagct
     4861 ggagatagcc gtcgaaggac ttcgagccct tggccgggac ggtaatgagg tcgcggaact
     4921 cccagtagtc cttcgtcggg agcttgatgc cctgggagtg ggcgatgccc cacatgatcg
     4981 ccggcgtgac cgcggcgccg agatggcagt gcagctcggc gagcggaacg gttctgggga
     5041 gctggcggac catcagcctc ggattctagc cgagccgcgc ttcgccaata tcgtgatcaa
     5101 gaaacgggca ccgccgcgcc cgtatcctcg cttcgaccga tcgcgcgatc ggagggagtg
     5161 gaggaaatcc cgtgcagatc tctagacgcc tgtcccgacg ccgcctggcg acagccgcgt
     5221 tcctggttgt cggactcctg gcgttcccgc tgggcgtgat cgcatcccac cagtacagcg
     5281 atgtgcctga ctcgaacccc tatcacgccg acatcgatgc catcaccgac gccggcgtca
     5341 cgaccggctg cggcaacgga tcgatgtact gccccagcgc cttcgtcacc cgcgagcaga
     5401 tggccgcgtt catgaaccgc ctgggcgccc tccaagcggg caagactccg gtcgtcaacg
     5461 cgaccaaggt ggacgggttc aacgccaacg atctccaccg ggtcgcggcg tacgtcgtgc
     5521 cgacgctgat cgactcctcg gcgatctcga cgaccgggac gctcgcgggc acgatcacga
     5581 tcccgcaaaa ggggtatctc atcgtcgcgg ccaacacgac gctctacacc cccgggaccg
     5641 ctggctcgac gacttgccag ttgcagttct cgggtggatc agcctggccg ggcacgtatc
     5701 accagacgac gacggggacc ccgatggtcg tgaaccagtg caccacccag gccgtcatcg
     5761 aggtctgcaa cccaggctcg ccctacaccg tcgagttcta cgtttcggcg ggaacgggtg
     5821 acacgtccgc ccagaacgca accctcatca tccagtacgt gccgttcgat ggagagggcc
     5881 tccggcctga ctgcctgggc tgagcccggg gagcccgtca gccggcgatc ggcaccaccc
     5941 ggccgtcggc gtcgagcgtc gcgcggcggc ggcgcagggt cttgagcgct gcgcccggga
     6001 cctggtcgcg ggcgtggtag ctcgagcgca cgagcggccc ggattcgacg tgcttgaagc
     6061 cgaggccaag ggcctcggtc ttcatctcgg cgaactcgtc ggggtggtag tagcgctcga
     6121 ggggcaggtg cttgagcgac ggccgcaggt actggccgat cgtgaggatg tccgtatcga
     6181 catcgcgcag cgcctcgaac gcctcggtca gctcgtcgcg cgtctcgccg agcccgacca
     6241 tcagcgagct cttggtatgc acggcgtacg gcgcctcggc cgcgtacgcc ttcgcgcgct
     6301 cgaggacgcc caacgatcga tcccagcgcg cgcgcttccg gaccggcttc tggagccggc
     6361 gaaccgtctc gaggttgtgg ttgaggatgt ccggccgagc ggccatgacg tcgcgcagcg
     6421 gcgcctcgtc cccgttgaaa tcggggatga gcacctcgac gcccatgccc ggcacctgct
     6481 cgtgcagcag ccggatcgtg tcggcaaaga cgtgcgcgcc gccgtcgggg aggtcgtcgc
     6541 gggcgaccga ggtgacgacc acgtgctcga ggccgagctg ctggatcgcc tcggcgacgc
     6601 gccggggctc atcgtcgtcg aaccaggtcg gcttgccggt cttgatattg cagaacccgc
     6661 aggcgcgggt gcaggtatcg cccaggatca tgatcgtggc cgtgcgctgg tcccagcact
     6721 cgccgatgtt cgggcagtgg gcctcttcgc agaccgtgtt gaggttcagg ccggtgagca
     6781 ggcgcttgag gtcgtggtag ttctcgcctg acggcagccg agccttgatc cactccggat
     6841 ggcgccgaag cgccgccgaa ccgtccgcgg ctgctccgaa tgcgccgccg cccatcgcga
     6901 tcgagatcgg ctcggccggg gcgtgtccgt ggccgcccgc gccgggcgga ttgaacatcg
     6961 ggagctcgcg attcatggcc atcgggcgct cggtgggagg cctcggcgcc ctagtagatc
     7021 gccgtgtcgg gcccgacggc ctcgagccag gccttgatgt cgcgcagcag cgcggcgccg
     7081 ccggcgccgt cgttcgctcg atggtcgacc ccgaggacca tgttcatgat cgagcggatc
     7141 ccgatgacgt cgccctcggg cgtctcgacg acgaccgggc gcttcgtgat ccggtccatc
     7201 gtcacgatgc cgacctcggg cacgttgatg atcggcatga ccatgttcgt gccgagccag
     7261 cccgtgttgt cgaccgtgaa cgtgccgccg ccgaagtcgt cgagctggaa tttgttcgcg
     7321 cgggctcgat cggacacctc ggcgatggct ttgttcaggc cgctgatcga gaggttgtcg
     7381 acgtcgcgga tcaccggcac gatgaggccg ccctcgacgg cgaccgcgat gccgatgttg
     7441 atgcgacgct tcgcgaggag cccgttctcg gtccagtggg cgttgaaggt cggattgcgc
     7501 ttgagggcct cggccgacgc cttgacgacg aacggcacga agctcaggga aattccttcg
     7561 cgggcctggt agcccttctt ctcgcgatcg cggaacctga ccaggctgga gacatcgacc
     7621 tccatctgga cgtaggcgtg gggcacctgg agagcgcggt tcatctgggc cgcgatgccc
     7681 ttgcgcatct gggtcatcgg gacgaggact tcgtcggcgc cttccgggaa ggcgatcgtg
     7741 gcgctcgact gggtggcggg tgcggctgcg cgcggggccg cggcgggtgc cggcccggcc
     7801 gatgtcgcag ctgcgggtgc ggcgggcgcg gcagctcccg cgggcgccga tgcggccggt
     7861 gtcgcggatg cgtcctgtcg cccaacgggc ttgctcgtgc gctggctctc gacgtagtcg
     7921 gtgacgtctt cgcgggtgat ccggccgccg ccgcccgtgc cgacgatcat cgccgcgctc
     7981 aggccgtgct cgcggaggag ccggcgaacc gcgggcgtca tgcgcgcatc ggcgctgccg
     8041 ggcagctcgt gcacgccgcg ctcgtccgag ccagccgggc cgttcgaggg ctgcgagacc
     8101 gcaggcgccg cagacgccat cggtgatgcc acggccgcac tggctggcgc ttcgggcgcc
     8161 ggtgccgcgg ctgatgcggg cgccggtgtg ggcgccgagg cggcaggagc aagggcagcg
     8221 gattccttcg gggctgcgcc ggagccgttg ctcggcgcag ccgaggtcga tgcggtcgcg
     8281 gccgtggccg ccggggctgc cgtcgaagcg gcagtcgatg ccgccgcgcc ggcctcctcg
     8341 atgatcgcga tctcggcgtt gttcgggacg gtggcgcctt cctcgacgag gatctccttg
     8401 aggatgccct cgtacgggga tggcacctcg gcgttgacct tgtcggtgat gacctcgagc
     8461 agcggctcgt acttggcgac gtgatcaccg ggctgcttga gccacttgcc gatggtgccc
     8521 tcggcgacgc tctcgccgag ctggggcatc gtcaccttcg ccacttggtc agttcctccg
     8581 atcgaacgcc gcgacacgcc gggagatcgc cgtgtgtcac cggctagtgt cgcgcatatc
     8641 ttccgaacgc gaacgatcta ggctcgatcc cagcgccgat ctggggtccg cgcgccactg
     8701 gaggagtccc tcgcgatgct caacctgctc gtcatcctcg ccggcctggt catcctgggc
     8761 gcgtcgctgc tgttcggtat cttcctgccg ctgagcaggc cggagccgcg gggcagcgat
     8821 ccacggtttg cccagcaacc acgcaggctg cacctgttga acgggatcgt cggtgcgacg
     8881 atcggcggga tcatcctcgt catcggcttc gccgggccgt tcgtcgaagt tcccgccggc
     8941 aacgtcggcg tcgtcaccaa cttcggctcg gtccagcggg tgacgctcga gcccggattg
     9001 cacgtcgtca tcccgatcgt ccagcacgtc accaacgtcg acacgcgcgt ccagccgcac
     9061 cacttccagg agatcgacgc cgcgtccaag gagctccaga cggtccggct caccggaacg
     9121 atgaactacc acatcgacgg ccagttcgcg aacgacctgt tccagcgcgt cggcaccgac
     9181 ttcgccgaga aggtcatcga cccggccttc aacgacttca tcaagacggt cgtcccgggc
     9241 tactcggtca acgagatcct ggccaagcgc gacgagattc gatcgctggc gaaggacctg
     9301 ctcggcgcga acctcgccca gtaccacatc atcgtggacg acatctacat cgcgaacatc
     9361 gccttctccg acgggttcca gcaggcgatc gaggacaagc aggtcgcgga gcagcgggtc
     9421 cagaccgagc agcagatctt ggcgcagcga cagatccagg cgcagcaggc cgtcgcccag
     9481 gcgaaaggac aggcagattc aatcttcacg ctcgccgaag gccagtccag ggcaaacgcg
     9541 ctcctcgcgg cctcgctgac cg
//
LOCUS       DATBSW010000030         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_989799, whole genome shotgun sequence.
ACCESSION   DATBSW010000030 DATBSW010000000
VERSION     DATBSW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989799"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VIF63_00880"
     CDS             complement(<1..780)
                     /locus_tag="VIF63_00880"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:NF014753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY8237960.1"
                     /translation="MKKLINDVANVVPEALTGFGRASGDIVSIDAANLVVLRAGGPVK
                     GKVALVSGGGSGHEPLHGGFVGTGMLDAACAGHVFTSPVPDQMEAATRAVDGGAGVLH
                     IVKNYTGDVLNFQLAAELVAGEVEVATVVTNDDVAVQDSLYTAGRRGVGGTVLVEKIA
                     GAKAEAGASLADVTATAVKVNAGARSMGMALTSCIVPAAGTPTFELGDDEIEIGIGIH
                     GEPGRRREKIAPVKDITRALLDPIVEDLGLGSGENVLAFVNG"
     gene            complement(826..1575)
                     /locus_tag="VIF63_00885"
     CDS             complement(826..1575)
                     /locus_tag="VIF63_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY8237961.1"
                     /translation="MSSPSFAQKLTAEAIGTAILVFIGAGSVPLTVFLTKGAGGTGID
                     GTAGLSTISFAFAFAIMAAVYSVGHISGCHINPAVTISLLVTRHIDSRTAGGYIVAQL
                     VGAFIGAGLTLIILTGNDPAALGLGAVSVNANAGLAIGFAAEVIGTAILVFTVYGSAV
                     SGKAPAGFAGIVIGFIVYGIIILVGPITGASLNPARELAPALLGQAIGAAPIPVDQLI
                     VVYVFGSTIGGVIGALLYQFIGKEQPAAAAA"
     gene            complement(1606..1875)
                     /locus_tag="VIF63_00890"
     CDS             complement(1606..1875)
                     /locus_tag="VIF63_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY8237962.1"
                     /translation="MPELRVVIKDPAGLHARPAARFVQAASRFESRIVIRHGDREADA
                     KSLIALLGLTIPPSSEIVLAADGPDADLALTSLAAELAPYLDQPD"
BASE COUNT          308 a          666 c          755 g          289 t
ORIGIN      
        1 gccgttgacg aaggcgagga cgttctcgcc cgagccaagc ccgagatcct cgacgatcgg
       61 gtccagcaac gcgcgggtga tgtccttgac gggcgcgatc ttctcgcgcc gccggcccgg
      121 ctcgccgtgg atgccgatgc cgatctcgat ctcgtcgtcg ccgagctcga acgtgggtgt
      181 ccccgccgcc ggcacgatgc acgacgtcag ggccatgccc atcgaacgcg cgccggcgtt
      241 gaccttgacg gccgtggcgg tgacatccgc cagcgacgcg cccgcttcgg ccttggcgcc
      301 cgcgatcttc tcgacgagga ccgtcccacc gacgccacga cggccggcgg tgtacagcga
      361 gtcctggacc gcgacgtcgt cgttggtgac gaccgtggcc acctcgacct cgcctgccac
      421 caattccgcg gcgagctgga agttgaggac gtcgccggtg tagttcttga cgatgtggag
      481 gacgcccgcg ccaccatcga cggcacgggt cgcggcctcc atctggtccg gcacgggcga
      541 ggtgaagaca tggccggcgc aggccgcgtc gagcatgccc gtcccgacga agccgccgtg
      601 gagcggctca tgaccggacc caccgcccga caccagggcc accttgccct tgaccgggcc
      661 cccggcccgc aggacgacga ggttggcggc atcgatgctg acgatgtcgc cggaggcccg
      721 accgaacccg gtcagcgcct cgggtacgac gttcgcgacg tcgttgatga gcttcttcat
      781 cgcgtggtca ctccttgtgg ggtcgtgctg gggaggatcg ggcggtcagg cggccgcggc
      841 ggccggctgc tccttgccga tgaactgata gaggagcgcg ccgatgacgc caccgatggt
      901 cgaaccgaag acgtagacga cgatcagctg gtcgaccggg atcggcgcgg cgccgatggc
      961 ctggccgagg agcgcgggcg cgagctcacg ggcggggttc agggaggccc cggtgatcgg
     1021 tccgacgagg atgatgatcc cgtagacgat gaacccgatg acgatgccgg cgaacccggc
     1081 gggtgccttg ccggacacgg ccgagccgta gaccgtgaac acgaggatgg cggtgccgat
     1141 gacctcggcg gcgaacccga tcgcgaggcc ggcgttggcg ttcaccgaga cggcgcccag
     1201 gccgagcgcg gccggatcgt tgccggtgag gatgatcagg gtcaggccgg ctccgatgaa
     1261 ggcaccgacg agctgggcga cgatgtagcc accggcagtc cggctgtcga tgtggcgggt
     1321 gacgagcagc gagatcgtga cggccgggtt gatgtggcag ccgctgatgt ggccgacgga
     1381 gtagaccgcg gccatgatcg cgaaggcgaa cgcgaacgag atggtcgaca gaccggcggt
     1441 gccgtcgatg ccggtgccac cggcgccctt ggtcaggaag accgtcagcg gcacggagcc
     1501 cgcgccgatg aacacgagga tcgcggtgcc gatggcctcg gcggtgagct tctgggcaaa
     1561 gctgggggac gacatggacc ttcctccttc gttggtcggt tcgactcagt cgggctggtc
     1621 gagatagggg gcgagctccg ccgcgagcga cgtgagcgcg aggtcggcgt ccggaccgtc
     1681 ggcggccagg acgatctcgg acgacggtgg gatcgtcagt ccgagcaggg cgatgaggct
     1741 cttggcgtcc gcctcgcggt cgccgtggcg gatgacgatc cggctctcga accgcgacgc
     1801 cgcctggacg aagcgggccg cgggccgggc gtgcagcccg gccggatcct tgatgacgac
     1861 acgcaactcg ggcatgtcag gctcgcggga gcttgggcat cgtggccgcc ccggcggcgg
     1921 ccgcctcgac ctcgtcgagc gtggccccga tgctggcctg gacggctgcc aggatcgcgc
     1981 cctcgaccag cggcgcctcg gtcacccgga cgcgctcg
//
LOCUS       DATBSW010000031         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_531239, whole genome shotgun sequence.
ACCESSION   DATBSW010000031 DATBSW010000000
VERSION     DATBSW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531239"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIF63_00895"
     CDS             <1..191
                     /locus_tag="VIF63_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237963.1"
                     /translation="PADAPGRLVRHDGRVAMTTIDGRLVFEGVQPAGGRPMTGDEFLR
                     GRASVVGSTVLAAGAGDR"
     gene            188..>1507
                     /locus_tag="VIF63_00900"
     CDS             188..>1507
                     /locus_tag="VIF63_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8237964.1"
                     /translation="MTATPIGSRVGAPRPRPDTPTKVRGATRYAADRSQQGLLHARLV
                     LSPRAHARIVSIDADAARALPGVVAVLTAADLPLKGEGSDRLSRPLAKSEVVFAGEPV
                     ALVVARTPAAAVDGAELVNIRFEALPPVVDAEGAMRADGPRARVELAEEGDRTGSMDA
                     STHAGVGGGSDDSIDAEVLNDNVTGRYRYREGDAAGAIGRAAVVKEGSFSTSWIHQGY
                     LEPQSCTAWVDVDDTLVIETSTQSLFGARSEVAKALGLPQHRVRAVMTPLGGGFGGKW
                     PLFDTLAAGAAWKLRRPVRLVATRTEDFAASNPGQPFATTIRIGASGDGILTGIEARV
                     LADAGAYEEGSAESLAGVLLAGPYAWPTFDIKAYGVRTNRFGVGAYRAPSAPAMCFAL
                     ETLMDEVAQALHIDPIELRRRNLVKVGDAMVDGEERWPVHGATEVLDA"
BASE COUNT          199 a          545 c          564 g          199 t
ORIGIN      
        1 agccggcaga tgcgcccgga cggctggtgc gtcacgatgg acgcgtggcg atgacgacga
       61 tcgatggccg gctcgtgttc gagggcgtgc agcccgccgg cggccgcccg atgaccggtg
      121 acgagttcct ccgcggtcgc gcgagcgtgg tcggctcgac ggtgctggct gccggggccg
      181 gtgaccgatg accgcgacgc cgatcggcag ccgggtcggc gcgccgcgcc cgcgcccgga
      241 caccccgacc aaggtgcgtg gcgcaacgcg ctacgcggcg gaccgctcac agcaggggct
      301 gctccacgct cggctcgtgc tgtcgccgcg cgcccacgcc cggatcgtct cgatcgacgc
      361 cgatgcggct cgtgccctgc cgggcgtcgt cgccgtgctc accgccgccg acctgccgct
      421 caagggcgag ggcagcgacc gcctgagccg gccgttggcg aagtccgagg tggtcttcgc
      481 gggagagccg gtggcgctcg tcgtcgcgcg cacgccggcc gcggccgtcg acggggcgga
      541 gttggtcaac atccggttcg aggccctgcc gccggttgtc gatgccgagg gcgcgatgcg
      601 cgccgacggg ccaagggcac gcgtcgagct cgccgaggag ggcgaccgaa ccggctcgat
      661 ggatgcgtcg acccacgccg gggtcggcgg cggcagcgac gattcgatcg acgccgaggt
      721 cctcaacgac aacgtcaccg gccgctaccg ctatcgcgag ggcgacgccg ccggcgccat
      781 cggccgggcg gcggtcgtca aggagggcag cttctcgacc tcctggatcc accagggcta
      841 cctcgagccg cagtcgtgca cagcgtgggt cgacgtcgac gacacgctgg tgatcgagac
      901 ctcgacgcaa tcgctgttcg gcgcccgctc cgaggtcgcc aaggcgctcg gcctgccgca
      961 gcaccgcgtc cgcgccgtca tgaccccgct gggcggcggc ttcggtggca agtggccgct
     1021 cttcgacacc ctggctgcgg gtgcggcctg gaagctccgc cggccggtcc ggctcgtggc
     1081 cacgcgcacg gaggacttcg ccgcctcgaa cccgggccag cccttcgcca cgacgatccg
     1141 gatcggcgca agcggcgacg gcatcctcac cgggatcgag gcgcgggtcc ttgcggacgc
     1201 cggcgcgtac gaggagggca gcgccgagtc gctcgccggt gtcctgctcg ccgggccata
     1261 cgcctggccg accttcgaca tcaaggccta cggcgtgcgc accaaccgct tcggcgtcgg
     1321 ggcgtatcga gcgccgagcg cgccggcgat gtgctttgcc ctcgagacgc tgatggacga
     1381 ggtcgcgcag gcgctccaca tcgacccgat cgagctccgc cgacgcaacc tggtgaaggt
     1441 tggcgatgcg atggtcgacg gcgaggagcg ctggccggta cacggcgcga ccgaggtcct
     1501 cgacgcg
//
LOCUS       DATBSW010000032         5871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_884131, whole genome shotgun sequence.
ACCESSION   DATBSW010000032 DATBSW010000000
VERSION     DATBSW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884131"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VIF63_00905"
     CDS             complement(<1..938)
                     /locus_tag="VIF63_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8237965.1"
                     /translation="MTDSPEDRDPGRARWRATTRKRALDAAPERRRFETSSGIEIGDL
                     YTPADIEGIDEERDLGRPGEPPFTRGVQATMYRSRLWTMRQYAGFSTAAETNKRFRYL
                     LEQGQTGLSVAFDLPTQMGFDSDAPEAEGEVGRVGVPISSLADMETLVDGIPLGEVST
                     SMTINATAATLVALYVAAAERQGVERSKVSGTVQNDILKEYIARGTWIYPPAPSMRLV
                     TDVFEFGARELPKWNTISISGYHMREAGATAAQELAFTLADAIAYVEAALARGMAIDD
                     FAPRLSFFFAAWSELFEEVAKFRAARRMWARIVKER"
     gene            complement(935..1606)
                     /locus_tag="VIF63_00910"
     CDS             complement(935..1606)
                     /locus_tag="VIF63_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8237966.1"
                     /translation="MSEQSNGTGGAGAYVGPGGRAGGDGQAGAGVAGAAEGDAGAAAR
                     AGAVRVTFPDGSPAMAVHAGPASQVALDGEVLEARLIPLGGGRSRLETAQGARDVIVT
                     ALPSATRATDGRERVEVVVDGWRFELDVEPEYRARLRERATSARDASARGGPLELHAI
                     IPGRVVSVDVAIGDTIDAGGRLLVIEAMKMQNELRAPRGGTISRLAVGPGQTVERGDL
                     LLVIE"
     gene            complement(1603..3186)
                     /locus_tag="VIF63_00915"
     CDS             complement(1603..3186)
                     /locus_tag="VIF63_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8237967.1"
                     /translation="MSALPFRRVLIANRGEIAVRIIRACRELGLETVAVYSDADRTAG
                     HVRLADTALRIGPPPAAESYLRMDAILAAAAESGAGAIHPGYGFLAERAEFARRVVAA
                     GLVYVGPSADAIEALGDKLHARRLARSAGVPVVPGTLEPAAIERPDQLGPLLDEAREI
                     GFPMLVKAAAGGGGRGMRRVERAEDLPTALLDGSHEAASAFGDGRVYLEREIAPARHI
                     EVQLLGDTTGRVIALGERDCSIQRRHQKLVEEAPAPGLTPAERETIHALAVSVATAAG
                     LTNAATAEFLRDEAGAFHFLEVNTRLQVEHGVTELVTGVDIVREQLFLAAGRPLSDGA
                     IEAAARASQPTGHAIEVRIAAEDPGSNFAPAAGPIRRWVMPAGPGVRVDTAIEAGDRV
                     PPEYDNLIAKVMVHAGDRDAAIDRLRRALGETEVGGIQTTLPFHRFVAAHDGFRRGEL
                     STDWVAANWDGAAESASARRLAAIVAGLVRHTPEGSAATPIDGVVGAATRAGDPTGSS
                     GGGAWAQAALEAATDRRPA"
     gene            complement(3217..4770)
                     /locus_tag="VIF63_00920"
     CDS             complement(3217..4770)
                     /locus_tag="VIF63_00920"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY8237968.1"
                     /translation="MPTNDERLAKLDAMRAESLLGGGADRVDKQHAMGKLTARERIEL
                     LLDAGSFTELDRFVVHRSTERGVIDNHPLGDGVVTGHGRIDGRVVYVFSQDFTVFGGS
                     LSEAYAEKICKVMDLAMKVGAPVIGLNDSGGARIQEGVASLGGYAEIFLRNVLASGVV
                     PQISVVLGPCAGGAVYSPAMTDFTVMVEGTSYMFVTGPSVVRTVTHEEVDSEFLGGAT
                     THTTRSGVAHLATPDEASALAAARQVLSYLPANNLERPTRQPAADADARLAAKLDDLI
                     PDEPQAPYDMHAVIGGVIDADSWMEIQPGWAQNVIVGFARLAGQAVGIVAQQPAVLAG
                     ALDIDASVKAARFVRTCDAFNVPLLTFVDVPGFLPGVDQEHGGIIRHGAKLLYAYCEA
                     TVPKLTVITRKAYGGAYDVMSSKHIRGDLNLAWPSAEIAVMGAAGAVAIIFKDALAAA
                     KDAETERARLVAEYETTFANPYIAAGRGYIDDVIRPSETRARLIAGLEALATKRDTNP
                     RKKHGNIPL"
     gene            5034..5804
                     /locus_tag="VIF63_00925"
     CDS             5034..5804
                     /locus_tag="VIF63_00925"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8237969.1"
                     /translation="MTNVIAIANQKGGVGKTTTAINLAGALAEDGLRILCIDMDPQAN
                     LTAGLGINLNTVEVSMADVLVGRATIDEIIRPTETPGVDIAPAHIDLAATEGELFTML
                     GRESLLRDALDGKLRKRYDYAILDCPPNLGLLTVNGLVAADGVIIPVQTQYYAMKGLT
                     NLVKVINTIRLKLNRRLKILGLLPTFFDGRTNLARDMLDELRVVGDHHVFTSIIRNTV
                     KLGEAPLVGRPITSYATSSEAARTYRELAREVVELGAN"
BASE COUNT          888 a         2095 c         1983 g          905 t
ORIGIN      
        1 aagcgctcct tcacgatgcg tgcccacatc cgacgcgccg ccctgaactt ggcgacctcc
       61 tcaaacagct ccgaccaggc ggcgaagaag aacgagagac gcggggcgaa gtcgtcgatc
      121 gccatccccc gggcgagcgc cgcctcgacg tacgcaatcg cgtccgcgag ggtgaaggcg
      181 agctcctggg ccgcggtggc ccccgcctcg cgcatgtggt agcccgagat cgagatcgtg
      241 ttccacttcg gcagctcgcg agcgccgaac tcgaacacgt cggtcacgag gcgcatcgac
      301 ggcgccggcg ggtagatcca cgtcccgcgg gcgatgtact ccttgaggat gtcgttctgg
      361 acggtgcccg agaccttcga gcgctcgacc ccctgccgct cggcggccgc gacgtacagc
      421 gcgacgagcg tcgccgccgt ggcgttgatc gtcatcgagg tcgacacctc gcccagcggg
      481 atgccgtcga cgagcgtctc catgtcggcg aggctcgaga tcgggacgcc gacgcggccc
      541 acctccccct ccgcctctgg cgcgtccgag tcgaagccca tctgggtcgg caggtcgaac
      601 gcgaccgaca ggccggtctg gccctgctcg agcaggtagc ggaagcgctt gttggtctcg
      661 gcggcggtcg agaagccggc gtactggcgc atcgtccaca gccggctgcg gtacatcgtg
      721 gcctgcacgc cgcgggtgaa gggcggctcg cccggtcgac cgaggtcgcg ctcctcgtcg
      781 atgccttcga tgtcggcggg cgtgtacagg tcgccgatct cgatcccgga tgaggtctcg
      841 aaccgccggc gctccggcgc ggcgtcgagc gcgcgcttcc gggtcgtcgc ccgccagcgg
      901 gcgcggccgg ggtcccgatc ctcggggctg tcggtcattc gatgaccagg aggaggtcgc
      961 cgcgctcgac cgtctggccg ggcccgacgg cgagccggct gatggtgccc ccgcgcggtg
     1021 ctcggagctc gttctgcatc ttcatggcct cgatgacgag caggcgccca ccggcatcga
     1081 tcgtgtcgcc gatggcgacg tcgaccgaca cgacgcgccc ggggatgatg gcatggagct
     1141 ccaacgggcc gccacgtgcc gaggcgtcgc gggcgctcgt cgcccgctcc cggagccggg
     1201 cccggtactc gggctcgacg tccagctcga accgccagcc gtcgacgacg acctcgaccc
     1261 gctcccgccc gtcggtcgcc cgggtcgcgc tgggcagtgc ggtgacgatc acgtcgcggg
     1321 cgccctgggc cgtctcgagc cgggacctcc ctccaccgag tgggatgagg cgggcctcga
     1381 ggacttcgcc atcgagcgcc acctggctcg ccggccccgc gtggaccgcc atcgccggcg
     1441 atccatcggg aaacgtcacc cggaccgccc cggctctggc ggcggccccg gcatcgccct
     1501 cggcggcgcc ggcgacacct gctccggcct ggccatcgcc tccggcacga ccgcctggac
     1561 cgacataggc accggcaccg cccgtgccgt tgctctgctc gctcatgccg gcctccgatc
     1621 cgtcgccgct tcgagggcgg cctgtgccca ggcccctccc ccactcgacc cggtcgggtc
     1681 gccggctcgg gtcgcagcgc cgaccacacc gtcaatgggc gtcgcggcgc tcccctcggg
     1741 cgtatgccgg acaagccccg cgacgatcgc ggcgaggcgc cgggcactcg cggactcggc
     1801 cgcaccgtcc cagttcgcgg ccacccagtc ggtcgagagc tcgccccgcc ggaagccgtc
     1861 gtgggccgcg acgaaccgat ggaacggcag cgtcgtctgg atcccgccga cctcggtctc
     1921 cccgagagcg cgccgcagcc gatcgatcgc cgcatcgcga tcgccggcat ggaccatgac
     1981 cttggcgatc aggttgtcgt actccggcgg aacccggtcc cctgcctcga ttgcggtgtc
     2041 gaccctgacg cccggccccg cgggcatcac ccaacgccgg atcggcccgg ccgctggggc
     2101 gaagttgctg cccggatcct cggcggcgat ccggacttcg atcgcatggc cggtcggttg
     2161 actggcccgg gccgcagcct cgatggcccc gtcggacaag ggccggcccg ccgcgaggaa
     2221 gagctgctcg cggacgatgt ccaccccggt cacgagctcg gtgacgccat gctcgacctg
     2281 gagtcgggtg ttgacctcga ggaagtggaa ggccccggct tcgtctcgca ggaactccgc
     2341 cgtcgccgca ttcgtgagcc ccgccgcagt cgccaccgat acggcaaggg cgtggatcgt
     2401 ctcgcgctcg gccggcgtga gacccggcgc cggcgcctcc tcgacgagct tctgatggcg
     2461 gcgctggatc gagcagtcgc gctcacccag ggcaatcacc cggccggtcg tatcgcccag
     2521 gagctggacc tcgatgtgcc gcgccggtgc aatctcgcgc tcgaggtaga cccgcccgtc
     2581 gccgaatgcc gaggccgcct cgtgggatcc atcgagcagg gccgtcggca ggtcctccgc
     2641 ccgctcgacc cggcgcatgc cacgcccgcc gccgcccgcc gctgccttta ccagcatggg
     2701 gaacccgatc tctcgcgcct cgtcgaggag cgggccgagc tggtcgggtc gctcaatcgc
     2761 tgcgggctcg agggtgcccg ggacgaccgg gacgccggca ctccgcgcga gtcttcgcgc
     2821 atggagcttg tccccgagcg cctcgatcgc gtctgccgac ggaccgacat agacgagtcc
     2881 ggcggccacg acccggcgag cgaactcggc gcgctctgcc aggaacccgt agcccggatg
     2941 gatggcgccg gcgcccgatt ccgccgccgc ggcgaggatg gcatccatcc gcaggtagct
     3001 ctcggcggcc gggggcggcc cgatccgcaa cgcggtatcg gcgagccgga cgtgacccgc
     3061 cgtccggtcg gcgtcgctgt agaccgcaac cgtttccagt ccgagctctc gacaggcacg
     3121 gatgatccgg accgcgatct cgccccggtt ggcgatgagg acgcggcgga acggcagggc
     3181 gctcatggcg tcgtctttgc gcaaagacgc ccttggctag agcgggatgt tcccatgctt
     3241 cttccgcggg ttggtgtcgc gcttggtggc gagggcctcg agacccgcga tgagccgggc
     3301 ccgcgtttcg gatggtcgga tgacgtcgtc gatgtagccg cggccggcgg cgatgtaggg
     3361 attggcaaac gtcgtctcgt actcggccac cagccgcgca cgctctgtct cggcatcctt
     3421 ggcagcggcg agcgcgtcct tgaagatgat cgccaccgcg ccggctgcac ccatcaccgc
     3481 gatctctgcg gacggccagg caaggttgag gtcgccgcgg atgtgcttgc tgctcatgac
     3541 gtcgtaggcg ccgccgtatg ccttgcgggt gatgacggtg agcttcggga ccgtggcttc
     3601 gcaataggcg tagagcagct tcgcgccgtg gcggatgatg ccgccgtgtt cctggtcgac
     3661 accgggcagg aaaccgggca catcgacgaa cgtcagcagc ggcacgttga aggcgtcgca
     3721 tgtcctgacg aatcgcgcgg ccttcaccga tgcatcgatg tcgagcgcgc cggcaaggac
     3781 cgcgggctgc tgcgcgacaa tgccgacggc ctgtcccgcg aggcgcgcga atccgacgat
     3841 gacgttctgc gcccaaccgg gctggatttc catccatgag tctgcgtcga tcacgccgcc
     3901 aatgaccgca tgcatgtcgt atggcgcctg cggctcgtcg gggatgagat cgtcgagttt
     3961 cgcggcgagc cgcgcgtcag catccgccgc gggctggcgc gtcgggcgtt ccaggttgtt
     4021 ggccgggaga tagctcagca cctgcctggc cgcagcaagg gcgctcgcct cgtccggcgt
     4081 cgcgaggtgc gcgacgccac tgcgggtcgt gtgcgttgtc gcgccgccga ggaattcgct
     4141 gtcgacctct tcatgggtca cggtgcggac gacactgggt ccggtgacga acatgtagct
     4201 cgtgccctcg accatcaccg tgaagtcggt catcgccggc gagtagacgg cgccacccgc
     4261 acatggcccg agaacaaccg agatctgcgg tacgacgccc gaggccagga cgttgcgcaa
     4321 gaagatctcg gcatagccac cgagcgacgc gacaccctcc tggattctcg cgccaccgga
     4381 atcgttgagg ccgatcactg gcgcgcccac cttcattgcc aggtccatga ccttgcagat
     4441 cttctcggcg tacgcctcgg acagcgatcc accgaagacg gtgaagtcct ggctgaagac
     4501 atagacgaca cgtccatcga ttcggccatg gccggtgacg acgccgtcac ccagcgggtg
     4561 attgtcgatg acgccgcgct cggtggagcg atggacgacg aatcgatcca gctcggtgaa
     4621 cgaaccagcg tccagcagga gctcgatacg ctcgcgcgcc gtcagcttgc ccatcgcgtg
     4681 ctgcttgtcg acgcggtccg ctcccccacc gagcagggac tcggcgcgca tcgcgtcgag
     4741 ctttgcgagg cgctcgtcat tggtgggcac gcgaatctcc gtccgtcttt gcgcaaggac
     4801 agggctgttt gggcgtcggc agacatcata ggtcgcgact gtggcgccgt tcgactatcc
     4861 acagttcggt gcacaactcg ggcatcctgt ggataagtag agagcgctgg aacaggctcg
     4921 ctgcgctagg atcgacatcg gcgtccgggc ctgcgccacg gcttcggcat cgccacaatc
     4981 cgcgctcctt tccgacagaa gccgccatcg cgagtgaacg aggtgaagcc ccggtgacga
     5041 acgtgatcgc gatcgccaat caaaagggcg gggttggcaa gaccacgacg gcgatcaacc
     5101 tcgccggagc gctcgccgag gatggcctgc gcatcctgtg catcgacatg gacccgcagg
     5161 cgaatctcac tgcgggcctc ggcatcaacc tgaacacggt cgaagtgtcg atggcggacg
     5221 tcctcgtcgg gcgcgcgacg atcgacgaga tcatccgccc aaccgagaca cccggcgtcg
     5281 acatcgcccc ggcccacatc gatcttgcgg ccaccgaggg cgagctgttc acgatgctcg
     5341 gacgagagtc gctcctgcgc gacgcgctcg atggcaagct gcgcaagcgc tacgactacg
     5401 cgatcctcga ctgcccgccg aacctcggcc tcctgacggt gaacgggctc gtcgccgcgg
     5461 acggcgtgat catccccgta cagacgcagt actacgcgat gaagggcctg acgaacctcg
     5521 tcaaggtcat caacaccatt cgactcaagc tgaaccggcg cctgaagatc ctcggcctgc
     5581 tacccacgtt cttcgacggc cgaacgaacc tcgcccgcga catgctcgac gagctgcgcg
     5641 tcgtcggtga ccaccacgtc ttcacctcga tcatccgcaa taccgtgaaa ctgggcgagg
     5701 caccgctcgt cgggcgcccg atcacgagct acgccacctc gtccgaggcc gcgcggacct
     5761 atcgcgagct tgcacgagag gtggtcgaac ttggcgcgaa ctgacttccg cgccgccgcc
     5821 gcgcggcgcc tgtcggagga gcgcgagctc tcgccggcaa tcgtgagcct c
//
LOCUS       DATBSW010000033         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_848999, whole genome shotgun sequence.
ACCESSION   DATBSW010000033 DATBSW010000000
VERSION     DATBSW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848999"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VIF63_00930"
     CDS             <1..273
                     /locus_tag="VIF63_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237970.1"
                     /translation="AARGERLAVVLLLLDALAPLAGAALSVVVTVTPATLGLLLATFA
                     GVFIAIGAGHLLPESQHHDPRRGPVMVLLSGLGAAIVLGIRAIAPV"
     gene            281..676
                     /locus_tag="VIF63_00935"
     CDS             281..676
                     /locus_tag="VIF63_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8237971.1"
                     /translation="MNDEGLALGPIMQILVPVADVEKAAAFYEDVLGLPLLFRFPGNA
                     FFDAHGVRLYLAQPSEPGYIGPVTLYFRVDDVSSTFERLVARGAAVREAPEITHRDAS
                     YDLWLAFVSDLDGNNIGLMREAPKGIERG"
     gene            complement(700..>1534)
                     /locus_tag="VIF63_00940"
     CDS             complement(700..>1534)
                     /locus_tag="VIF63_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8237972.1"
                     /translation="AWSAQLRPEALSEGVGSLLDAVEAPFQPGPPPDPDQVVGISVIV
                     GQVGAALITLLALVFFWLTERPRLQRYALAFMPAHRRSGIRDAWNEVESRLGLWVRGQ
                     LVLMATIGVATGIAYSVLGLPAALLLALIAALTEVIPIVGPLLGAIPAVLIATTISPE
                     TAVVTFGIYVILQLVEGNVLVPMVMRNTVGLSPFLVLVSLLVGGSVGGILGAVVAVPV
                     VAGITVILERLQDREVPVPIDPAADRTPDEAARDELESIAPGSPAPKRRAPRRSPARS
                     T"
BASE COUNT          234 a          542 c          549 g          209 t
ORIGIN      
        1 gccgcgcgcg gcgagcgtct cgccgtcgta ctgctcctgc tcgacgcact tgccccgctt
       61 gccggcgccg ccctcagcgt cgtcgtgacg gttacccccg cgacgctcgg actgctgctg
      121 gcaacctttg ccggcgtgtt catcgcgatc ggggcgggcc acctgctgcc cgagtcgcag
      181 caccacgacc cgcgccgcgg cccggtcatg gtcctcctgt cgggcctcgg cgcagcgatc
      241 gtgctcggca tccgcgcgat cgcaccagtt tgagacccag gtgaacgacg aaggcctggc
      301 gctcggaccg atcatgcaga tcctggtccc ggttgccgat gtcgagaagg cggcggcgtt
      361 ctacgaagac gtcctgggcc tgccgctgct cttccggttc ccgggcaatg cgttcttcga
      421 cgcgcatggc gtgcggctgt acctggccca gccgagcgag ccgggctaca tcggcccggt
      481 cacgctgtat ttccgggtcg acgacgtgag ctcgacgttc gagcggctgg ttgcccgcgg
      541 cgcagccgtc cgcgaggcgc ccgagatcac ccatcgcgac gcgtcgtacg acctgtggct
      601 ggcgttcgtc agcgacctcg acggcaacaa catcgggctg atgcgcgagg cgccgaaggg
      661 aatcgagcga gggtagccgc cgagatcacg cgccccggat caggtggagc gggcaggtga
      721 acggcggggc gcgcgacgct tcggtgcagg cgatccgggg gcgatggatt cgagctcgtc
      781 tctggccgcc tcgtcgggcg tccggtccgc agccgggtcg atcgggaccg gcacctcgcg
      841 atcctgcaac cgctcgagga tgacggtgat gccggccacg accgggacgg cgacgaccgc
      901 cccgaggatg ccgccgacgc ttccgcccac gagcaggctg accaggacca ggaacggcga
      961 gagccccacc gtgttgcgca tgaccatcgg gacgagcacg ttgccctcga cgagctgcag
     1021 gatgacgtaa atcccgaagg tcacgaccgc cgtctccggc gagatggtcg tggcgatgag
     1081 cacggccggg atcgccccga ggagcggccc gacgatcggg atgacctccg tcagcgcggc
     1141 gatcagcgcg agcagcaggg ccgccgggag accgagcacg ctgtacgcga tcccggtcgc
     1201 gacgccgatc gtggccatca gcacgagctg gccgcgcacc cagaggccga ggcgcgactc
     1261 gacctcgttc caggcatccc ggatcccgga ccggcggtgc gcgggcatga acgccagggc
     1321 gtagcgctgg aggcgtggcc gctcggtcag ccagaagaag acgagcgcga ggagggtgat
     1381 cagcgcagcc ccgacctggc ccacgatcac cgagatcccg accacctggt ccgggtcggg
     1441 cggcggaccg ggctggaacg gcgcctcgac ggcgtcgagc agggagccaa caccctcgct
     1501 cagcgcctcc ggccggagct gcgcgctcca ggcc
//
LOCUS       DATBSW010000034         5566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_637451, whole genome shotgun sequence.
ACCESSION   DATBSW010000034 DATBSW010000000
VERSION     DATBSW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637451"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VIF63_00945"
     CDS             <1..1335
                     /locus_tag="VIF63_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY8237973.1"
                     /translation="RAFGLLSTIVAIFFIRGKETENPMNMLNRGYWVTTILSVFALLL
                     TTNVMLQTNGNAPSGIPVWVYFFGAGVVGLATSVAFVYITQFYTAGSYRPVREIAEAS
                     RTGPATNIISGTAVGYETTFVTAITIGIALIASHWLGSQAGIKNAAGHDVGGIFGTAV
                     ATMAMLMTTAYILAMDTFGPITDNAGGIAEFSRAEAGAREITDRLDAVGNTTKALTKG
                     YAIASASLAAFLLFSAYIDKVNLIHTNRNEPLLTAVNLANVDVFVAALIGAMLVYFFS
                     SLAIRAVGKTAQSIIVEVRRQFREMPGIMDYTQRPDYARVVDITTKAALREMIAPGVV
                     AVATPIIVGLLLGAEAVAGMLMVGTIAGVMLATVLNNGGGAWDNAKKYIESGNLKDEN
                     GKVLGKGSDAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAPLFIR"
     gene            1365..2204
                     /gene="uppP"
                     /locus_tag="VIF63_00950"
     CDS             1365..2204
                     /gene="uppP"
                     /locus_tag="VIF63_00950"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEY8237974.1"
                     /translation="MDSQLVQAIVLGIVQGLTEFLPVSSSGHLIIVPELLGWRDPFIE
                     SLAFSVMLHVATLLALLVYFRADWLRLIPAGLAAIRDRSFKDDPDRRLAWLLAASTVP
                     AVIVGVLFNDLIENSFREARLVAITLVIGAAVLWLADRVGRRDRPIEGMTFPIAIGIG
                     AAQALALVPGISRSGISISAGLFAGLDRTAAARFAFLMATPITAGAGVWELRKIVSGE
                     AGVDLPLVPLAAGMLASLIAGLLAITVLMRYLRTHGTGVFVAYRIALAALVVVAWLGL
                     WDR"
     gene            2229..2690
                     /locus_tag="VIF63_00955"
     CDS             2229..2690
                     /locus_tag="VIF63_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEY8237975.1"
                     /translation="MKQLRQRAIRDLVEQRPIRTQQELAAALRERGFRATQATISRDV
                     AELGLGKGSREGRAAYVLPPRLRDVEVSGEDRLRRLLSDVPVEFRVADLMLVIRTLPG
                     SAHAVAAALDRTRWPEIIGSIAGDDTVFVATGDKGSLRRLRDRLMGLSGRH"
     gene            2843..3307
                     /gene="greA"
                     /locus_tag="VIF63_00960"
     CDS             2843..3307
                     /gene="greA"
                     /locus_tag="VIF63_00960"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8237976.1"
                     /translation="MNTKPTYISKDGLEKLRHELEELVSSRRPEIAQRIHDAKEHGDL
                     TENAEYEDAKNEQAFVEGRIQLVEALIKNATLIDEHTTNDHVQIGSTVKVDGPDGSQS
                     FTIVGSTEAKPTEGRISNESPVGRALLGKKKGENVTVSVPAGELDYKILAIS"
     gene            3323..4936
                     /gene="lysS"
                     /locus_tag="VIF63_00965"
     CDS             3323..4936
                     /gene="lysS"
                     /locus_tag="VIF63_00965"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8237977.1"
                     /translation="MEWADELAAKVSGPQVVNDSKTPSGTVHVGSLRGPVILDVITRA
                     LRAAGRETTLLYGVDDLDPMDAQALLTPDAVEREMGRPLAHIPDQAGDGHASYARHHA
                     QAFIDTFAGLGVHPDRYYWMSEHYAAGDMDPFITIALDRAEIVRAVYRRVANVQHPDT
                     WHPISVICPTCGRVGTTIATKWDGQKVFFECREQLVTWAKGCGASGWVAPYGGAAKLP
                     WNLEWAAQWSLFGVTIEPCGKDLATAGGSRDRSDAIAREVFEREPPINVPYEFLNIGG
                     KKMSTSKGLGASAQQMTEVVPPEQLRLLFLRPRPNHAIEFDPIGTDAVPRIFDEFDRL
                     GAAVAGRDVKGDLPQGFERVFQYSLLDPNADLDAEAASFRFPFAHLATLIQIPGVDVA
                     ASAAAEKGEPLAEHEQAILEERIRAARSWLDTYAPDAAKLVVQSTLPEAAAELDAMDR
                     AFLAGLLEDAQDLAKRSLHADGEAWQTAIFESAAAAGLKPGRAFRAVYLAFLGRPNGP
                     RAGLLLAKLEQDFVEGRLREAAAGGTLPA"
     gene            4947..>5566
                     /locus_tag="VIF63_00970"
     CDS             4947..>5566
                     /locus_tag="VIF63_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237978.1"
                     /translation="MSVGLARLREEPDAIRKGAVDKGEDPAIVDEAIQADSDWRNAKA
                     ELESLLMERNSASKAIGQLMASGVAPDDPRIADLKTQSTRAAERRELLETSVGYFEKK
                     LEDLLLRIPNPAEADVPIGGEEANVTIRVWGNQLPRAAYKEGPDEFPDADPDKETWDR
                     KPHWEIAEALDIIDLPRGAKVAGSGFPLYKGAGAALQRALITWFLDV"
BASE COUNT          885 a         1999 c         1841 g          841 t
ORIGIN      
        1 cgtgccttcg gtctcctctc gacgatcgtg gccatcttct tcatccgcgg caaagagacc
       61 gagaacccga tgaacatgct caaccgcggc tactgggtca cgaccatcct gtcggtcttc
      121 gccctcctgc tgaccacgaa cgtcatgctc cagacgaacg gcaacgcgcc atcgggcatc
      181 ccggtgtggg tctacttctt cggcgccggc gtcgtcggcc tcgcaacgag cgtcgcgttc
      241 gtctacatca cgcagttcta cacggccggg tcctaccggc ccgtccgcga gatcgccgag
      301 gcgtcccgga ccggtccggc gacgaacatc atctcgggca cggcagtggg ctacgagacc
      361 accttcgtga ccgcgatcac gatcggcatc gcgctcatcg ccagccactg gctcgggtcg
      421 caggcgggca tcaagaacgc cgccgggcat gacgtgggcg gcatcttcgg caccgccgtc
      481 gcgacgatgg cgatgctcat gaccacggcg tacatcctcg cgatggacac cttcggcccg
      541 atcaccgaca acgccggcgg catcgccgag ttctcgcgcg ccgaggcggg cgcccgcgag
      601 atcaccgacc ggctcgacgc cgtcggcaac accacgaagg ccctgacgaa gggctacgcg
      661 atcgcctcgg cgtcactcgc ggcgttcctg ctgttcagcg cctacatcga caaggtcaac
      721 ctgatccaca ccaaccggaa cgagccgttg ctcaccgcgg tcaacctggc caatgtcgac
      781 gtgttcgtcg cggccctcat cggcgcgatg ctcgtctact tcttcagctc gctcgccatc
      841 cgcgccgtcg gcaagacggc ccagtcgatc atcgtcgagg tccgccgcca gttccgagag
      901 atgcccggga tcatggatta cacgcagcgg cctgactacg cccgcgtcgt cgacatcacg
      961 accaaggccg ccctgcgcga gatgatcgcc ccgggcgtcg tcgcggtcgc aaccccgatc
     1021 atcgtcggcc tgctgctcgg cgccgaagcc gtcgcgggca tgctgatggt cggcacgatc
     1081 gcgggcgtga tgctcgctac cgtgctgaac aacggcggcg gcgcgtggga caacgccaag
     1141 aagtacatcg agtcgggcaa cctcaaggac gagaacggca aggtgctcgg caagggctcc
     1201 gatgcccacg ccgccgctgt cgtgggtgac acggtcggcg atccattcaa ggacaccgcc
     1261 ggtccatcgc tgcacgtcct cgtgaagctg ctcgcgacga tcacgctggt tctcgcgccc
     1321 ctgttcattc gctgatccgg cccgggcccg gataggatcg cccggtggac agtcagctcg
     1381 ttcaggcgat cgtgctcggg atcgtgcagg gactcaccga gttcctgccc gtatccagct
     1441 ctgggcacct gatcatcgtc ccggagctct tgggttggcg cgacccgttc atcgagagcc
     1501 tcgcgttcag cgtgatgctc cacgtggcga cgctgctcgc gctcctggtg tacttccgag
     1561 ccgactggct gcgactgatc ccggccggtc tcgccgcgat ccgggatcgc tcgttcaagg
     1621 acgacccgga ccggcggctc gcgtggctcc tcgccgcatc gacggtgccc gcggtgatcg
     1681 tcggtgtcct gttcaacgac ctcatcgaga actcgttccg tgaagcgcgc ctggttgcga
     1741 tcaccctcgt gatcggcgcg gcggtgctgt ggctggccga tcgcgtaggt cggcgcgacc
     1801 ggccaatcga gggcatgaca ttcccgatcg cgatcggcat cggggcggcg caggccctcg
     1861 cgctcgtgcc gggcatcagc cgctcgggca tctccatctc ggccggcctg ttcgccgggc
     1921 tcgatcgcac ggccgcggcc cgcttcgcgt tcctcatggc cacgccgatc accgcgggcg
     1981 cgggcgtgtg ggagctgcgc aagatcgtgt ccggcgaggc cggcgtcgac ctgcccctcg
     2041 tcccgctcgc cgcgggcatg ctggccagcc tcatcgccgg actgctcgcc atcacggtgt
     2101 tgatgcgcta cctgcgcacg catgggaccg gggtcttcgt cgcgtaccgg atcgcgctcg
     2161 ccgccctcgt cgtcgttgcg tggctcggcc tctgggatcg ttgagcgggc ggtagcccca
     2221 tggaagtgat gaagcagctc cgccagcgcg cgatccgcga cctggtcgag cagcgcccga
     2281 tccgcaccca gcaggagctc gccgccgccc tccgcgagcg cggcttccgc gcgacgcagg
     2341 cgaccatcag ccgcgacgtc gccgagctcg ggctcggcaa ggggagtcgc gaggggcgcg
     2401 ccgcatacgt gctcccgcca cgcctccgcg acgtcgaggt ctccggcgag gatcgcctcc
     2461 ggcgcctcct ctccgacgtg ccggtcgagt tccgcgtggc cgatctcatg ctcgtcatcc
     2521 gcaccctgcc tggaagcgcc catgccgttg cggccgcgct ggaccgaacc cgctggcccg
     2581 agatcatcgg ctcgatcgcc ggcgatgaca cggtgttcgt ggccaccggc gacaagggct
     2641 cgctgcgtcg cctccgcgac cgcctcatgg gcctgtccgg gcgccactga ccggggcggg
     2701 caacagactc ggcgtgagga cgaatcgggc ggctttgaca ccccccgagg cccgccgtag
     2761 tattcgcggc cgacccggac tccagccttc cagcgaatac gcagggctgg ggttctttcc
     2821 ttatccgggg tacgcgaaca ccatgaatac caagccgacc tacatcagca aagacggcct
     2881 cgagaagctc cgccacgagc tcgaggagct cgtgtccagc cggcggcccg agatcgccca
     2941 gcgcatccac gacgccaagg agcacggcga cctcacggag aacgccgagt acgaggacgc
     3001 caagaacgag caggcgttcg tcgagggccg catccagctg gtcgaggcgc tcatcaagaa
     3061 cgcgaccctg atcgacgagc acaccacgaa cgaccacgtc cagatcggct cgaccgtcaa
     3121 ggtcgatggc ccggacggct cgcagagctt caccatcgtc ggctcgaccg aggccaagcc
     3181 gaccgagggc cggatcagca acgagagccc cgtcggacgc gccctgctcg gcaagaagaa
     3241 gggcgagaac gtcaccgtct ccgtgcccgc cggcgagctc gactacaaga tcctcgcgat
     3301 ctcgtaggag gctcgtacag ccatggagtg ggctgacgag ctggcggcaa aggtctccgg
     3361 accgcaggtc gtcaacgact cgaagacccc gtccggaacg gtccacgtcg ggagcctgcg
     3421 cgggccggtc atcctcgacg tcatcactcg cgcgctgcgc gcggcgggcc gtgagaccac
     3481 gctcctgtac ggcgtcgacg acctcgaccc gatggatgcc caggcgctcc tgacccccga
     3541 tgcggtcgag cgcgagatgg gccgcccgct cgcccacatc cccgaccagg cgggcgacgg
     3601 gcacgcctcg tatgcccggc accacgcgca ggcgttcatc gacacgttcg cgggcctcgg
     3661 cgtccacccc gatcgctact actggatgag cgagcactac gccgcgggcg acatggaccc
     3721 gttcatcacg atcgcgctcg accgcgcgga gatcgtccga gcggtgtacc ggcgcgtcgc
     3781 gaacgtgcag caccccgaca cgtggcaccc gatctcggtg atctgcccga cctgcggccg
     3841 cgtcggcacc acgatcgcga cgaagtggga tgggcagaag gtgttcttcg agtgccgcga
     3901 gcagctcgtg acctgggcga agggttgcgg cgcgtcgggc tgggtcgcgc cgtacggcgg
     3961 ggcggcgaag ctgccctgga acctcgaatg ggctgcgcag tggtcgctgt tcggggtgac
     4021 gatcgagccg tgcggcaagg acctcgcgac ggccggtggc tcccgtgacc gatccgatgc
     4081 gattgcgcgc gaggtgttcg agcgcgagcc gccgatcaac gtgccctacg agttcctcaa
     4141 catcggcggc aagaagatgt cgacctcgaa ggggctcggc gcgtccgccc agcagatgac
     4201 cgaggtcgtg ccgcccgagc agcttcgcct gctcttcctc cggccccgtc cgaatcacgc
     4261 catcgagttc gatccgatcg ggaccgacgc cgtgccccgc atcttcgacg agttcgatcg
     4321 gctcggcgcc gccgtcgccg gccgcgacgt caagggcgac ctgccgcagg gcttcgagcg
     4381 tgtcttccag tactcgctgc tcgacccgaa cgccgacctc gatgccgagg ccgcgagctt
     4441 ccggttcccg ttcgcccacc tcgcgacgct catccagatc ccgggcgtcg acgtggcggc
     4501 gagcgccgcc gcggagaagg gcgagccgct ggccgagcac gagcaggcga tcctcgagga
     4561 gcgcatccgc gccgcgcgct cgtggctcga cacctacgca ccggacgccg ccaagctcgt
     4621 tgtccagtcg accctgcccg aggcagccgc cgagctcgat gcgatggacc gggcgttcct
     4681 ggccgggctg ctcgaggacg cgcaggacct cgcgaagcgc tcgctccacg ccgatggcga
     4741 ggcgtggcag accgcgatct tcgaatccgc tgccgcggcc ggcctcaagc cgggacgcgc
     4801 gttccgcgcc gtctacctcg ccttcctcgg gcgtccgaac gggcctcgcg cgggcctgct
     4861 gctcgcgaag ctcgagcagg atttcgtcga ggggcgcctg cgggaggccg ccgccggcgg
     4921 cacacttccg gcatgacggt gctggcatga gcgtcgggct cgcgcggctt cgcgaggagc
     4981 cggacgcgat ccgcaaaggc gcggtcgaca agggcgaaga ccccgcgatc gtcgacgagg
     5041 cgatacaggc cgactccgac tggcgcaacg ccaaggccga actcgagtcg ctactcatgg
     5101 agcgcaattc cgcgagcaag gccatcggcc agttgatggc gtctggcgtg gcgccggatg
     5161 accccagaat tgcagacctg aagacgcaat cgacgagagc cgccgagagg cgcgagctcc
     5221 tcgaaacatc cgtcgggtac ttcgagaaga agctcgaaga cctgctcctg cgcatcccga
     5281 accccgccga ggccgacgtg ccgatcgggg gcgaggaggc gaacgtcacg atccgggtct
     5341 ggggtaacca gctgccgcgg gccgcgtaca aggaaggccc ggacgagttc ccggacgccg
     5401 atcccgacaa ggaaacgtgg gatcggaagc cgcactggga gatcgccgag gcgctggaca
     5461 tcatcgacct gccccgtggc gccaaggtcg ccggctcggg cttcccgctg tacaagggcg
     5521 ccggcgccgc cctccagcgc gccctgatca catggttcct cgacgt
//
LOCUS       DATBSW010000035         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_567401, whole genome shotgun sequence.
ACCESSION   DATBSW010000035 DATBSW010000000
VERSION     DATBSW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567401"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VIF63_00975"
     CDS             <1..972
                     /locus_tag="VIF63_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237979.1"
                     /translation="KLGDWPFGAEVAAIVADVAGAMVVRDLIPLADFQTVLTPWTWRP
                     DAALEPAAAGIRAAGIRAWGLTAVGEGPGSIVRGSRRSTAFGGAAVALVAIVGLAMLT
                     GGPTHPLLAVAGFTDAPTATPIPVVAGIASPLASSAFIDPSPVMSPDPSVASPTPTES
                     GPTTSPNGPLPHPTAGPAPTAGPGPTSGPAPTPGPTLKPTPPPTLVPTPVPTLVPTPV
                     PTPVPTPVPTPVPTPVPTPVPTPVPTPVPTPVPTPSPSPGVICAVINLVNVNSANAQL
                     AWTTAGFTGTVHFVTPIPPQYKVVWQSLTVGAGVPCTSDINVDRVAP"
     gene            1122..1325
                     /locus_tag="VIF63_00980"
     CDS             1122..1325
                     /locus_tag="VIF63_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY8237980.1"
                     /translation="MTTGTIKKVVADRGFGFIAAEDAKEYFFHRGALDSSLTFERLNG
                     GEKVEFEIEQSPKGARAAKVRAA"
     gene            1481..2671
                     /locus_tag="VIF63_00985"
     CDS             1481..2671
                     /locus_tag="VIF63_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237981.1"
                     /translation="MPAPQPITALADSAAPTTRLYAINRRHLDELTGEHGIFQYAAGK
                     LPDPTRGHSLDDVARALRVDILHSRTLGWHAVAESARRNLRFLEDAFDEPSGRFMSFR
                     QADGQWVEGLGSNDSLGRAMLALGETIAGAHDARLVERAIVLFGRALKQAGKVVTPRA
                     QASVILACAAMTQTRVLMASAEERDATIDTEAAATLRRMATGLHARFLDFAQPGWPWP
                     EEALTFENALLPHALILAGGRIRAETMQRVGLQILDWLIAAQTAPEGHLSPVGTGTWV
                     HRGEKAKFDQQPIEATALILAAEAAYTATGDPRYAAAMEQAYAWFLGANDLKRQVARP
                     ARGASSDGLTATGASPNESAEATLMWLMASEHMRLFRGQPVRARVATPGRRSTAPALA
                     PTPV"
     gene            complement(2676..2951)
                     /locus_tag="VIF63_00990"
     CDS             complement(2676..2951)
                     /locus_tag="VIF63_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8237982.1"
                     /translation="MSGRRYRVDIAPAAQRQFSRLPPGVAASLRGPVLALALEPRPPG
                     VSKLSGSDLWRVRVGDLRLIYAIDDAALLVIVLRVARRSESTYRRVR"
     gene            complement(2948..3205)
                     /locus_tag="VIF63_00995"
     CDS             complement(2948..3205)
                     /locus_tag="VIF63_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237983.1"
                     /translation="MKATFDIEPELYRAVKVEAARLDRSVRDVVAEALQAWLDEAEER
                     EDRESASAALEEYRRSGGEAAESWFARMAAETKAAYGTDEG"
     gene            complement(3264..>3600)
                     /locus_tag="VIF63_01000"
     CDS             complement(3264..>3600)
                     /locus_tag="VIF63_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237984.1"
                     /translation="TGDGFFVGFDSPEQAVAAAVAIQRRLAEHRQKAGFAPPVRIGLH
                     AADANVIGDNYRGRGVHEASRIASLAKGGEIVASASTAGDTYPTSAPRSEILKGFSEP
                     VEVVTVDWK"
BASE COUNT          529 a         1360 c         1171 g          540 t
ORIGIN      
        1 aagctcggcg actggccgtt cggcgctgag gtagcggcaa tcgtggccga tgtcgcgggt
       61 gcgatggtcg ttcgcgacct gatcccgctg gccgatttcc agacggtcct cacgccctgg
      121 acctggcgcc ccgacgcggc actcgaaccc gccgcggcgg ggatccgcgc ggcggggatc
      181 cgcgcgtggg gcctcacggc cgtgggcgag ggccccggtt cgatcgtacg cggctctcga
      241 cgctcgactg ccttcggcgg tgcggcggtt gcgctggtgg cgatcgtcgg gctcgcgatg
      301 ctcacgggcg gtccgacgca tccgctcctc gccgtcgcgg gcttcaccga tgctccgacc
      361 gccacgccca tccccgtcgt cgccggcatt gcgtcgccgc tcgcgtcgtc ggcattcatc
      421 gacccgagcc ctgtcatgag tcccgatcct tcggtggcgt cacccacccc gaccgagtcc
      481 ggaccgacga catcgccgaa cggcccgctc ccccacccga ccgcaggtcc tgcgcccact
      541 gccggtcctg ggccgacctc tggtcccgcg cccactccgg gacccaccct gaagcccacg
      601 ccgcccccga cgctcgtgcc gacaccggtg ccgacgctcg tgccgacacc ggtgccgacg
      661 cccgtgccca cacccgtgcc gacgcctgtg cccacccccg tccccacccc cgtgccgacg
      721 cctgtgccca cccccgtgcc gaccccggtt cccacgccga gcccgtcacc cggcgtgatc
      781 tgcgcggtca tcaacctggt caatgtcaac tcggccaacg cccagctcgc ctggaccacg
      841 gcagggttca cgggtacggt gcacttcgtg acaccgatcc cgccccagta caaggtcgtc
      901 tggcaaagcc tgacggtcgg tgctggcgtg ccgtgcacga gcgacatcaa cgtcgaccgg
      961 gtcgcgccct aggcgtttcg cattgcagct aggaaatttc gaacaaatgt gctagggtta
     1021 ccgcactatc gcaactcgcg atggtgcttc gcgtggtcgt ccaacttttc gaacggaccg
     1081 tgcgttctgc agttccagtt cgggaagtgt tggaggtcct catgaccacc ggtaccatca
     1141 agaaggtcgt tgccgatcgc ggcttcggct tcatcgccgc cgaggatgcg aaggaatact
     1201 tcttccatcg cggcgcgctc gattcttcgc tcaccttcga acgcctcaac ggcggcgaga
     1261 aggtcgagtt cgagatcgag cagagcccca agggcgcacg cgcagccaag gttcgcgcgg
     1321 cctaagccct tgagacccgc accgtgcggg tcgacaactc tcgagccggt cgcgggcgcg
     1381 atttcgatcg ccgccacggc cggctcgttc atcactgcga gcgcagttgc gctcgtttcc
     1441 tcgggaccgc ggcgcgcgga cgctcgagtg gagaccaccg atgcccgccc cccagccgat
     1501 cacggccctc gcggatagcg ccgcgccgac gacgcgcctg tacgcgatca atcgacgcca
     1561 cctggacgag ctgaccggcg agcacgggat cttccagtac gcagcgggca agctccccga
     1621 cccgactcgc ggccacagtc tcgacgacgt cgcgcgggcc ctgcgggtcg acatcctcca
     1681 ttcgcgaacg ctgggctggc atgccgtcgc cgagagcgcg cgccgcaatc tccgcttcct
     1741 cgaggacgcg ttcgacgagc ccagcgggcg attcatgagc ttccgccagg ccgacggcca
     1801 gtgggtcgag ggcctcggct ccaacgacag cctgggccgg gcgatgctcg ccctgggcga
     1861 gacgatcgcc ggtgcccacg atgcacggct ggtcgagcgc gccatcgtcc tcttcgggcg
     1921 cgcactgaaa caggccggca aggtcgtcac gccccgggcg caggcgtccg tcatcctggc
     1981 ctgcgcggcc atgacccaga cccgggtcct gatggcgtct gccgaagagc gagacgccac
     2041 catcgatacc gaggccgccg cgacgctccg acgcatggcg acgggcctcc acgcccggtt
     2101 cctcgacttc gcccagcccg gctggccgtg gccggaagag gcgctgacct tcgagaatgc
     2161 gctcctgccg cacgcgctca tccttgccgg cggtcgaatc cgcgccgaga cgatgcagcg
     2221 cgtcgggctc cagatcctcg actggctgat cgcggcgcag accgccccgg agggccatct
     2281 gtcgcccgtc gggaccggca cctgggtgca tcgcggcgag aaggccaagt tcgaccagca
     2341 gccgatcgag gcgaccgcgc tgatcctcgc cgccgaggcc gcctacaccg ccacgggcga
     2401 tccacgctac gccgcggcca tggagcaggc gtacgcctgg ttcctcggtg cgaacgacct
     2461 caagcgacag gtcgcccgcc cggcgcgcgg cgcgagctcc gatgggctga ccgccacggg
     2521 cgcgagcccg aacgagagcg ccgaggcgac cctgatgtgg ctgatggcgt cggagcacat
     2581 gcggctattc cgtggccagc ccgtgcgcgc ccgtgtcgcg acacctggcc gccgatccac
     2641 cgcaccggcg ctggcgccga cccccgtctg atcagctacc gcactcggcg gtaggtgctc
     2701 tccgagcgtc tcgccacacg caggacgatg acgagcagcg ctgcatcgtc gatcgcgtag
     2761 atcagccgca gatcaccgac acggacgcgc cacaggtccg aacctgagag cttcgacacg
     2821 cccggcgggc gaggttccag cgcgagggca agcacgggac cgcggagcga cgctgcgacg
     2881 ccgggaggga gccggctgaa ctggcgctgc gcggctgggg cgatgtcgac ccgatagcgg
     2941 cgcccgctca cccctcgtcg gtgccgtagg ccgccttggt ttcggcggcc atccgggcaa
     3001 accaggattc cgccgcctca ccgccgctgc gacgatattc ctccaatgcc gccgaggccg
     3061 actcgcgatc ctcccgctcc tccgcttcgt ccaaccacgc ctgcagcgcc tcggcaacaa
     3121 cgtcgcggac gctgcgatcc agccgagcgg cctccacctt gacggcgcga tagagctcgg
     3181 gctcgatgtc gaaggttgct ttcatcggcc cagtatgctg gcttgctggc ttcctgccaa
     3241 gcccctcacg agatcccgcg gctctatttc cagtcgacgg tgacgacctc gaccggctca
     3301 gagaagcctt tcaggatctc gcttcgaggc gccgacgtcg ggtaggtgtc gccggccgtc
     3361 gaggcgctcg cgacgatctc gccgcccttg gccagcgagg cgatccgcga cgcctcgtgg
     3421 accccgcggc cgcggtagtt gtcgccgatc acgttcgcgt ccgcggcgtg caacccgatg
     3481 cggaccggcg gggcgaagcc ggccttctgt cggtgctccg cgaggcgccg ctggatcgcg
     3541 acggccgcgg cgacggcctg ctcgggcgag tcgaacccga cgaagaaccc gtcgccggtc
//
LOCUS       DATBSW010000036         7655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1096495, whole genome shotgun sequence.
ACCESSION   DATBSW010000036 DATBSW010000000
VERSION     DATBSW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096495"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..620)
                     /locus_tag="VIF63_01005"
     CDS             complement(144..620)
                     /locus_tag="VIF63_01005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8237985.1"
                     /translation="MRKSISAIVVAVAASLILPAAAFAHWPVANVRSYISQGYSREHK
                     AYDIASYKGTKVVPIRAGTVVFAGRKDNCGGLQVYIRHGDGLYSAYHHLSAEYVHRGQ
                     YVTGRTMIGRVGESGCATGPHLHISTWRGFPWHSGSFRISPWTYIDSGSYLPLRYR"
     gene            893..1624
                     /locus_tag="VIF63_01010"
     CDS             893..1624
                     /locus_tag="VIF63_01010"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY8237986.1"
                     /translation="MESGGSTAITWYGHACVELVSPGGVTVLFDPWFANPVSPRSPDS
                     VERCDVMLVTHGHADHIGNAIPIASRTRPAWPAMHELVLWLSSVYSAPNDLIGMNKGG
                     TAEVRGLRITMTSADHSAGDWDNASGAPRYLGEPAGFVVELEDGSRVYHAGDTNVFGD
                     MRLIGELYRPDLAILPIGGHYTMGPREAALAIELLGVREVLPIHYGTFPILAGTPDAL
                     RDELAARSLGDVVVHGIQPGETLRR"
     gene            1761..4250
                     /locus_tag="VIF63_01015"
     CDS             1761..4250
                     /locus_tag="VIF63_01015"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY8237987.1"
                     /translation="MASQRRSEDVLHPEHQPHDVSRDPILDGRDSLRLVKLRLALTLI
                     AVAVLPIAAVSPLVRAVAEEARVTHQDRLADLAVTAVAEVEREVEIVRADTESFLNDP
                     LIIAAARSDAKAADRQVASGRLAQLAARSANAQSGSIVTAVALLTADGAVAGYGNSPD
                     LTALPPGVLVSGFASLGGADGVDGVDGRGDSRLVVVEAGAKPARTIVTTISVRALLAA
                     ATPDTEMPGRTLRMTDATGALLALSQGPFDPSSLPGEMLDLATMAAHDSDGRATVILP
                     GIEGWQVVVSAPIPLVEMPAQALAALALMLLLLVVFTMWMARQILRPAAQLEASRSRL
                     RELYESEREAGLRDSLTGLGNHRAFQEAVARMVEGARRYGTAFSLVLIDIDEFKRVND
                     TRGHAVGDQLLAEVGQLIGETIRGTDAAFRVGGDEFALLLPHTDVGGSVILTRRLLAR
                     GLEDRVHGDYRAPISFSAGVTACPEFGTTRLEITAQADAALYRGKRAGRTVVTIYDPE
                     KDHGHVDEGMRAELSSAISALIEARSLTPVYQPIVDLNSGRVIGYEGLVRVPKQSIFA
                     HAGALFDAAEVAGRVSDLDRAALDIVLGGAYDLGENLSLSVNVSPRTLESPEFSSTAF
                     LSILRRHGMAPGRIVLELTEREAIRDIERLRVALRGVQAAGARIAADDVGAGNAGLRL
                     LSQFRFDVVKIDLSLVQRAGEDKTHSVLRSLVEMADRLGATTIAEGVETSSQLRTARR
                     LGITAGQGYLLGRPGPERDLAWVDIAALEERDEIAIPITAELPDRRAKPVPWAPLPEP
                     LDTRTAVASGPLAAMRRRSAGLLRAGRAAED"
     gene            complement(4282..5985)
                     /locus_tag="VIF63_01020"
     CDS             complement(4282..5985)
                     /locus_tag="VIF63_01020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY8237988.1"
                     /translation="MASPSPRTAADLLVACLEAEACEYVFSVPGEETLDLLEALSRSD
                     AIRHITTRHEQGAAFMADVYGRLTGRAAVAMATLGPGATNLLTGIADAYLDRAPVVAI
                     TGQASSEKLHKEAHQVVDIVDMFKPVTKWNARIERTAVIPEIVRKAFRVAMLAKPGPT
                     HIELPENIAGTELAADDPIRPLVPGKAYFPEPSHDAIAHAARLINEAERPIILAGNGV
                     LRRKASGELRAFAKGLHLPVAATFMGKGAVDDRSHLSLMAVGLQARDHVLTGFDRADL
                     VIAVGYDLVEYAPAYWNPDGVKRIIHIDTQPAEVDGDYQPEIELIGDIGGALEELLAA
                     TLPGGVGGRSATERHESRETLVHADLRTALLADLQAGESSDGYPITPQRAIYDLRRAL
                     GPDDIVVSDVGAHKVWIARLYQAYEPNTVIISNGFASMGIALPGAIAAKLVHPDRHVV
                     ALCGDGGFMMNAQELETAKRIGAGIVVVIWRDNGYGLIDWKQRNAFGRPFGVEFDNPD
                     LVKFAESFGIPGYRPATADELYPTLMKALDQPGPSVIDVPIDYAENVRLTERLGALAG
                     L"
     gene            6094..6300
                     /locus_tag="VIF63_01025"
     CDS             6094..6300
                     /locus_tag="VIF63_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237989.1"
                     /translation="MIHRKPAQTTRYGVSRSLRGAPIAMTVAIAVALGACTTPGATAS
                     PTDAMMEHSPSPTDAMMEHSPGPS"
     gene            6316..7512
                     /locus_tag="VIF63_01030"
     CDS             6316..7512
                     /locus_tag="VIF63_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8237990.1"
                     /translation="MIAIALAFASGILTIVSPCTLPLVPVVLGSRADPYRWTRVAATV
                     IGLGATFVVATVAIASLLAGVGVSLASARVFAAVVLGVAGLSLALPGLERWVERRLSP
                     LAALGDGRLRAVDRGGRPSRARSALRGALVGSLLGLVWAPCVGPLMAGVIATATIEGP
                     TLGGLSVALAYVAGTSAALVLVAAAVGRLRVRLPGKASAAVAARRATGIAMALVAVLV
                     VSGLDTRIGITLQPDALVTSATKGAGNNVTQSDDLGPAPELAGITAWINTGPTTLAAH
                     RGEVVLVHFWTFGCSNCQNVQPYVKDWYARYRDRGFTVLAVHTPELSFERDLDNVRAA
                     VAAKGVAYPVAVDPDFATWRAYGNHYWPAFYFVDRGGHVRRVHFGEGDYAASEAVIEQ
                     LLAEPA"
BASE COUNT         1062 a         2725 c         2672 g         1196 t
ORIGIN      
        1 gcgacgacgg cagcgaatga cccgacgccg agggccgtca ggatggtcca gttgatgaac
       61 gccaggctct cggcgatccg ctccgcgggc acgtcggcga gtgtagccgc gggctcggac
      121 cggaccgccg agcggccccg gcatcagcgg tagcgaaggg gcaggtacga ccccgaatcg
      181 atgtaggtcc acggcgagat tcggaacgaa cccgaatgcc acggaaaccc ccgccaggtc
      241 gagatgtgca ggtgcgggcc ggtggcgcaa ccgctctccc cgacgcgacc gatcatcgtc
      301 cggccggtga cgtattggcc ccggtgcacg tactcggcgc tgaggtggtg gtatgccgaa
      361 tagaggccgt ccccgtgccg gatgtagacc tggagacccc cgcagttgtc cttgcgaccg
      421 gcgaagacca cggtccccgc tcggatcggc acgaccttgg tgcccttgta cgaggcgatg
      481 tcgtaggcct tgtgctcgcg actgtagccc tggctgatgt aggagcggac gttggcgacc
      541 ggccagtgag cgaatgcggc cgccggcagg atgagactgg cggcaacggc caccacgatg
      601 gcgctgatgg atttgcgcat aagagtttgt gggcacgccg tggcgaggcc cgagctcctt
      661 tcctcctgga gccccccggt gagcgaggga caacgacgca tggcccgtcg acggcgcgcg
      721 cggagcgcca cgcggcgaaa caccgcgacg aggttgtctg cttctcctgc cgatgtgctg
      781 ggtgccacgg ctgcggggtg agcccgcgga gacgtccgca ttctgcctgc gagcacgcgg
      841 tttgtcagtc acccgatagg ctctgtcgat acgattgcgt cggaggtgtg acatggagtc
      901 cggcggatcg acggccatca cctggtatgg gcatgcctgc gtcgagctcg tgtcgcccgg
      961 tggcgtcacc gtgctcttcg atccgtggtt cgcgaacccg gtcagcccgc gctcccccga
     1021 cagcgtcgag cgttgcgacg tgatgctcgt cacccacggc cacgccgacc acatcggcaa
     1081 cgccatcccg atcgccagcc ggacgcggcc cgcgtggccg gcgatgcacg agctcgtcct
     1141 gtggctcagc agcgtgtact ccgctccgaa cgacctcatc gggatgaaca agggcggcac
     1201 cgccgaggtg cgcggcctgc gcatcacgat gacctcggcg gatcactcgg cgggcgattg
     1261 ggacaacgcg agcggtgcgc cgcgctacct cggcgagccg gcgggcttcg tcgtcgagct
     1321 cgaggacggc agccgcgtct accacgccgg ggacaccaac gtgttcggtg acatgcgcct
     1381 gatcggcgag ttgtaccggc ccgacctcgc gatcctgccg atcggcggcc actacacgat
     1441 gggtccccgc gaggccgcgc tggcgatcga gctgctcggt gttcgcgagg tgctcccgat
     1501 ccactacggc acattcccga tcctcgcggg cacgccggat gcgctccgcg acgagctcgc
     1561 ggcgcggtcg ctcggcgacg tggtcgtcca cggaatccag ccgggcgaga ccctccggcg
     1621 ctgatcgtcg accccgccgc gcgacatgtg cggcgccgag cgagcccgag cggcgccaca
     1681 cgccacggcg acgcccgacc cgaggtccgg gtggacgcgg gacgaacggg taggtgcagt
     1741 tcagggcgga gtggtgcctc atggcatctc aacgacgctc cgaggacgta ttgcaccccg
     1801 agcaccagcc tcatgacgtc tcgcgcgatc cgatcctgga cggccgcgac agcctccggc
     1861 tggtcaagct gcgccttgcg ctgaccctca tcgccgtcgc ggtcctgccg atcgcggctg
     1921 tctcgccgct cgtgcgcgcg gtcgccgagg aggcgcgggt cacccaccag gaccggctgg
     1981 ccgacctggc cgtcaccgcc gtcgccgagg tggagcgcga ggtcgagatc gttcgggccg
     2041 acaccgagtc attcctgaac gacccgctga tcatcgccgc cgccaggtcc gacgcaaagg
     2101 cggcagatcg ccaggtggcc tcgggccgcc tcgcccagct cgccgcccgc tcggcgaatg
     2161 cccagtccgg cagcatcgtc accgccgtcg ccctgctcac cgcggacggt gcggtcgccg
     2221 gctacggaaa ctcgccggac ctcaccgcgc tgccaccggg cgtgctcgtc tccgggttcg
     2281 cgtcgctggg tggtgcggat ggcgtcgatg gcgtggatgg ccgcggtgac agtcgcctcg
     2341 tcgtcgtcga ggccggcgcc aagccggcgc gaacgatcgt gaccaccatc tccgtgcgcg
     2401 cgctgctcgc cgcggcgacc ccggacaccg agatgccggg caggacgctg cgcatgaccg
     2461 acgcgacagg tgcgctcctc gcgctgtccc agggcccgtt cgatcccagc tcgctgcccg
     2521 gtgagatgct cgacctggcg acgatggccg cccacgattc ggacggccgc gcaaccgtca
     2581 tcctgcccgg catcgagggc tggcaggtcg tcgtgtcggc cccgatcccg ctggtcgaga
     2641 tgcctgccca ggcgctcgcc gccctcgccc tgatgctcct gctgctggtc gtcttcacga
     2701 tgtggatggc gcgccagatc ctgcgcccgg cggcgcagct cgaggcctcc cggtcccgcc
     2761 tccgggagct gtacgagagc gagcgcgagg ccgggctccg tgacagcctg accggcctcg
     2821 gtaaccaccg cgccttccag gaggcggtcg cccggatggt cgagggcgcg cgccgctacg
     2881 ggaccgcatt ctccctggtc ctcatcgaca tcgacgagtt caaacgcgtc aacgacacgc
     2941 gtggccacgc cgtcggcgac cagctgctcg ccgaggtcgg ccagctcatc ggcgagacga
     3001 ttcgcggcac cgatgcggcc ttccgggtcg gcggcgacga gtttgccctc ctgcttccgc
     3061 acaccgacgt cggcgggtcg gtcatcctga cccgacggct cctcgcccgc ggcctcgagg
     3121 accgggtgca cggcgactac cgcgccccga tctcgttctc cgccggcgtg accgcctgcc
     3181 ccgagttcgg cacgacgcgc ctcgagatca ccgcgcaggc cgacgctgcc ctctaccggg
     3241 gcaagcgggc cggtcgcacc gtcgtgacga tctacgaccc cgagaaggat cacggtcacg
     3301 tcgacgaggg catgcgcgcc gagctttcga gcgccatcag cgccctcatc gaggcccgct
     3361 cgctcacgcc cgtgtaccag ccgatcgtgg acctgaactc cggccgggtc atcggctacg
     3421 agggcctcgt ccgcgtcccg aagcagtcga tcttcgccca cgccggtgcg ctgttcgatg
     3481 ccgcggaggt tgccggtcgc gtgtcggacc tggaccgcgc cgccctcgac atcgtccttg
     3541 gcggcgctta cgacctcggc gagaacctca gcctcagtgt caacgtgtcg ccccgaaccc
     3601 tcgagtcgcc ggagttcagc tccaccgcct tcctgtcgat cctgcgccgc cacggcatgg
     3661 ctccaggtcg catcgtcctc gagctcaccg agcgcgaggc gatccgcgac atcgaacgcc
     3721 tgcgggtcgc cctgcgcggt gtccaggccg caggcgcgcg aatcgccgcc gacgacgtgg
     3781 gcgcgggcaa cgccggcctc cggctgctca gccagttccg gttcgacgtc gtcaagatcg
     3841 acctctcgct ggtccagcga gcgggcgagg acaagaccca ctcggtgctc cgctcactcg
     3901 tcgagatggc cgatcgactc ggcgccacca cgatcgccga gggcgtcgag acctccagcc
     3961 agctgcggac ggcgcgacgg ctgggcatca ccgcgggcca gggctacctc ctcggccgcc
     4021 ccgggcctga gcgcgacctg gcgtgggtcg acattgccgc tctcgaggag cgcgacgaga
     4081 tcgccatccc gatcaccgcc gagcttcccg atcgacgagc caagccggtg ccctgggctc
     4141 cgctgcctga gccgttggac acccgcaccg cggtcgccag cgggccgctt gccgcgatgc
     4201 gacgccgctc cgccgggctc ctgcgagccg gccgagccgc agaggactga tgccggcggg
     4261 ccgcggcgcg accgccgcgc gctacaggcc cgcgagcgcc cccagccgtt cggtcagtcg
     4321 aacgttctcc gcatagtcga tcggcacgtc gatcaccgac ggcccgggct ggtccagtgc
     4381 cttcatcagc gtcgggtaca gctcgtcggc cgtcgcgggc cggtacccgg gaatcccgaa
     4441 cgactcggcg aacttgacga ggtccgggtt gtcgaactcg accccgaagg gccgcccgaa
     4501 ggcgttgcgc tgcttccagt cgatgaggcc gtagccgttg tcgcgccaga tcaccacgac
     4561 gatgcccgcc ccgatccgct tcgcggtctc gagctcctgc gcgttcatca tgaagccacc
     4621 gtcgccgcac agcgcaacga cgtgccggtc cgggtggacg agcttggcag cgatcgcacc
     4681 aggcaacgcg atgcccatcg atgcgaatcc gttcgagatg atcaccgtgt tcggctcgta
     4741 cgcctggtac agccgggcga tccagacctt gtgcgcgccg acgtcgctga cgacgatgtc
     4801 gtcaggcccg agcgcccgcc gcaggtcgta gatcgcgcgc tggggcgtga tcggatagcc
     4861 atcgctcgat tcaccggcct ggaggtcggc caggagcgcg gtccgcaggt cggcgtggac
     4921 gagggtctcg cgcgattcgt ggcgttccgt tgcgctgcgt ccgccgacgc cgcccggcag
     4981 cgtggccgcg agcagctcct ccagcgcgcc accgatgtcg ccgatgagct cgatctcggg
     5041 ctggtagtcg ccgtccactt cggccggctg ggtgtcgatg tggatgatcc gcttcacgcc
     5101 gtcagggttc cagtacgccg gcgcgtactc gacgaggtcg tacccgacgg cgatcacgag
     5161 atcggctcga tcgaacccgg tcaggacgtg gtcgcgggcc tgcagaccca ccgccatcag
     5221 cgacaggtgt gatcgatcgt cgacggcgcc cttgcccatg aacgtggcag cgaccggcag
     5281 gtggagaccc ttcgcgaacg cgcgcagctc gcccgaggcc tttcgacgga ggacgccgtt
     5341 gccggcgagg atgatcggcc gctcggcctc gttgatgagc cgcgcagcgt gggcgatcgc
     5401 atcgtggctc ggctcgggaa aataggcctt gccggggacc agcgggcgga tcgggtcgtc
     5461 ggcggccagc tcggtgccgg cgatgttctc gggcagctcg atgtgggtcg gtccgggctt
     5521 cgcgagcatc gccacccgga acgccttgcg gacgatctcg gggatcacgg cggttcgctc
     5581 gatgcgggcg ttccacttcg tcacgggctt gaacatgtca acgatgtcga cgacctggtg
     5641 ggcctccttg tggagcttct cggacgacgc ctggccggtg atcgcgacga cgggcgctcg
     5701 gtccaggtag gcgtcggcga tgccggtcag caggttcgtg gcgcccggcc ccagcgtggc
     5761 catggccacc gcggctcggc ctgtcaagcg gccgtagacg tcggccatga acgccgcgcc
     5821 ctgctcgtgg cgagtcgtga tgtggcggat ggcatcgctg cgcgacagtg cctcaaggag
     5881 gtcgagcgtc tcctcgcccg gcaccgagaa gacgtactcg caggcctcgg cctcgaggca
     5941 ggcgacgaga aggtccgcgg cagtgcgcgg cgacgggctg gccacgcgcc gcagtgtacg
     6001 gtcgccgcgc cgcaggctcg ccgctcgccc cgtgtcatcg tcgagacatc cggcctcgtc
     6061 atcctcgggc cgaacccgca tgaggcgcaa gccatgattc accgcaaacc ggcgcagacc
     6121 acccgttacg gcgtgagccg atccctgcgc ggcgcgccga tcgccatgac agtcgcgatt
     6181 gccgtcgccc tcggcgcctg caccaccccg ggtgcgaccg cctccccgac cgacgcgatg
     6241 atggagcatt cgccatcgcc gaccgacgcg atgatggagc actcccccgg tccgagctga
     6301 ggtcaggccg gcatcgtgat cgcgatcgcg ctggcgttcg ccagcggcat tctgacgatc
     6361 gtttcgccgt gcacgctgcc cctcgtgccg gtcgtgctcg ggtcgcgagc tgatccgtat
     6421 cggtggactc gggtcgcggc gacggtgatc ggcctcgggg cgacgttcgt cgtcgcgacc
     6481 gttgccattg cctcgctgct cgcgggcgtc ggcgtgtcac tcgcgtcggc gcgagtgttc
     6541 gccgcggtcg tgctcggcgt ggccggcttg agcctcgcgc taccggggct cgagcggtgg
     6601 gtcgagcgcc ggctctcgcc cctggcagcg ctgggggacg ggcggctgcg cgcggtggac
     6661 cgtgggggcc gcccaagtcg cgcgagatcg gcgctgcgcg gcgcgctcgt cgggtccctg
     6721 ctgggccttg tctgggcgcc gtgcgtgggg ccgctcatgg ccggcgtcat tgccacggcc
     6781 acgattgagg gcccgaccct cggtgggctt tcggtcgcgc tggcctatgt cgccggtacc
     6841 tcggccgcgc tcgtgctcgt ggccgcggcc gtggggcggc tccgggtccg cctcccgggc
     6901 aaggcctcgg cggcggtcgc cgctcgacgg gcgaccggga tcgcgatggc cttggtcgcg
     6961 gtgctcgtgg tcagcggcct ggatacgcgc atcgggatca ccctgcagcc tgatgcgctc
     7021 gtcacatccg caacgaaagg agccgggaac aacgtgacgc aatccgacga cctgggaccg
     7081 gcgccggaac tggccgggat cacggcctgg atcaacaccg ggccgacgac gctcgccgca
     7141 catcggggcg aggtcgtgct cgtccatttc tggacgttcg gctgcagcaa ctgtcagaac
     7201 gttcagccgt acgtgaagga ttggtacgcg cggtaccgcg accggggctt caccgtgctc
     7261 gccgtgcaca cgcccgagct ctcgttcgaa cgcgacctcg acaatgtccg ggcggccgtg
     7321 gccgcgaaag gcgtcgcgta tccggtggcg gtcgatccgg acttcgcgac gtggcgggcg
     7381 tacggcaacc actactggcc cgcgttctat ttcgtcgacc ggggcggtca tgtccggcgc
     7441 gtgcatttcg gcgagggcga ctacgccgcg tcggaggcgg tgatcgagca actccttgcg
     7501 gagccggcct gactactgcg gtgcggcgag caccatgatc cgcagctcgg tcatgtcctc
     7561 gatcgggcgt agggtcaccg cctggtcagt cgaccaggat gggctcttct gatgcggcgc
     7621 tccggcgagg aggttcgccc tctggtgggc cttca
//
LOCUS       DATBSW010000037         8070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1061144, whole genome shotgun sequence.
ACCESSION   DATBSW010000037 DATBSW010000000
VERSION     DATBSW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061144"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VIF63_01035"
     CDS             <1..627
                     /locus_tag="VIF63_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY8237991.1"
                     /translation="EVARIVRMGFELARTRRKIVTQVDKANVLATSRLWRRVTDEIAV
                     EYPDVTLGHQLVDSCAMLLIRRPAEFDVIVTENLFGDILSDEAAVLAGSLGVLPSASL
                     GERRTEHGTFGLYEPIHGSAPDIAGSDVADPIGTILSAAMLLRTSLGLEAAAAAVEAA
                     VDRALTDGWRTADLADPRDGTDGLVVVGTTGFTTAVIEALEADAGVAA"
     gene            624..2276
                     /gene="cimA"
                     /locus_tag="VIF63_01040"
     CDS             624..2276
                     /gene="cimA"
                     /locus_tag="VIF63_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY8237992.1"
                     /translation="MSAAADAPVVLYDTTLRDGTQGENITLSLADKLRIARMLDEFGM
                     PFIEGGWPGSNPKDIEFFAAARSMRWERAKLAAFGSTRHRANKPADDPNLRELVAAET
                     PVVTIFGKSWLLHVTEVLGASPTENLDMIAESVRLIVDEGRELVYDAEHFFDGYKANQ
                     AYALSTLKAAHESGARTLVLCDTNGGTLTGELLAILDATRAAIGADAVTWGIHTHNDA
                     ELAVANSLAAVQAGVRHVQATINGYGERTGNANMVSVLAGLALKTDLPLAPVGGGDLS
                     ALTELSRAVAEIANQAPNDYQPYVGRSAFAHKGGVHGAAFAKVERSYQHIDPTAVGNE
                     GRLVVSELGGRANTSIRARQLGHQLDGVIEPAVLSTLIKKLESEGLSFEGAEASFELL
                     IRRHQADYTPPFRVVDYTCLVEQRSGAELRAEATVKVEVDGELLHTAAEGNGPVNALD
                     AALRKALRAFYPVLDTVHLYDYKVRIIDSGSATGARTRVTIESTDGSAEWSTMGSDTN
                     IIAASATALADSLEYAIWKRGAELRRRDERHFTTVIAGEGAS"
     gene            2273..2809
                     /locus_tag="VIF63_01045"
     CDS             2273..2809
                     /locus_tag="VIF63_01045"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEY8237993.1"
                     /translation="MSAADAATTASQALELTRWTVTSGSNVQSRGAVVIDAGNHHWRA
                     SAEGNGAVDALFNAVDAALAEVLDGRPRLVGYDVHALGQGHEAEGLVMVAVEPPVGLE
                     GGRSGGLFQGTARSTNIVASSIEAYIEALNSMLAEPAWAGAAESAAKDRRKGPETHGR
                     RGEIDRSAKDDTAPWFDR"
     gene            2820..3683
                     /locus_tag="VIF63_01050"
     CDS             2820..3683
                     /locus_tag="VIF63_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEY8237994.1"
                     /translation="MKSISVAYSGEPGAFAEDAVLAWFAAPEPVPVQSFRAVFEAVRD
                     GSAPAGVIPVESSLLGTIRETLDLLYEFDLAIVGEVSVPVRLALLALPGERLDTIRTV
                     QSIAAALAQADEFLRSGSWTVETTWNTAGGARAIADRRQRGAAAVASPRAAALYGLEV
                     IADDIQTGSDNRTRFAVICRHAAGLDVQAAAAAAAIVGAPKTTLVFGVGNVPGSLHRS
                     LGAFAGRGINLSRLESRPWTGRGSRWEYLFWADLDADPTAPACAAALAELRAEAEMVR
                     VLGTYPRAAED"
     gene            3764..5440
                     /locus_tag="VIF63_01055"
     CDS             3764..5440
                     /locus_tag="VIF63_01055"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY8237995.1"
                     /translation="MDEAFRTEMADGYKLDEPALTLGSPLLGDEVLPDVRVQIALSRI
                     NRHGLVAGATGTGKTKTLQILAGQLSALGVPVFAVDVKGDVSGIGAPGDPADPKVTAR
                     CASIGWNFVAGGHPLEVLSLSGNKGAHVRASVSSFGPLLLGKVLDLNDTQTSILSLVF
                     RYCDDQHLPLLDLADLRTTLKFLAGDDGKAVLEDLGGISSASLGVILRAIVTIEQEGA
                     DIFFGEPEFDVNDLLRTTLDGRGMVTLLEVADVMDRPRLYSTFVLWMLAQLYETLPEI
                     GDVPKPKLAFFFDEAHLLFKDASEALLEQIERTARLIRSKGVGVYFVTQAPTDVPSSV
                     LSQLGNRIQHALRAFTPDDADALRKASRTFPVTTHYDVERTITSLGIGEALVTVLSPK
                     GVPTPLAATRLIPPDSRMAPLTDQELSQLVGASQLQARYGTTVDRESAHEIITARLAA
                     AHQAAAAAAGVPVAPPGSDPGAPYGGMTPAQYRREIERQAREQVKQQERERKAAERER
                     NAQERERKAAERERQRTINTGVRTAGRVITSRVGQSLIRGVFDTIFGGKR"
     gene            5600..>8070
                     /locus_tag="VIF63_01060"
     CDS             5600..>8070
                     /locus_tag="VIF63_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8237996.1"
                     /translation="MPAVRSSTRTASTFVVAAVLAWLTFPPLVPSTRAADIAPSADAA
                     LASLADLPSNFVDELVFSGVTNPTNIEFATDGRVFVAEKSGRVLVFDNLSDTTATVFA
                     DLRTQTHNYWDRGLLGLALHPNFPATPWVYVSYTYDAAIGGTAPRWGTAGQTGDPCPN
                     PPGATTDGCVVSSRVSRLTANGNASIGETVLVEDWCQQFPSHSIGDLEFGPDGALYVS
                     GGDGADFNVVDFGQKGSPLNPCGDPPSGVGGVQTSPSAEGGALRSQDLRTSGDPVGLA
                     GSILRIDPDTGAGLPGNPLFGNADPNARRIIAEGLRNPFRFTFRPGTDELWLGDVGWN
                     TWEEVDRITDTTPNDPVNFGWPCYEGNGRQPGYDAANLTVCETLYGASGAVQSPFFTY
                     SHSLDIAPPDDCPPAAPPAPVSSSVTGLAFYTGTAFPAIYSGALFGADYSRNCIWVMR
                     ADGNGVPDPSTIQGFQPAAQGPVVLKMGPDGSLYYANFNGGIRRIRYTVNLPPTANMT
                     ASPLGGPAPLDVSFDGTTSTDPEGHALSYAWDLDDDGTFNDGTGSTAAFTYTTTGQHR
                     ARLRVTDPGGEVDTEQVFIQVDNSLPAISITAPSASLQWSVGEDIAFAGTATDAQDGD
                     ISNQIDWDLTLFHCQGVLCHTHAITSWQDTAGATFSAPDHEYPSYLELKATVTDSSGA
                     TVSVTRRIDPKTVALTFLTSPAGLTVTVGGTSPQAAPFTRTMIVGSDNTVAVESPQLT
                     GGGTSYTFASWSDGGGLSHSITAPATATSYTANFTPTAPSSCPDTLPVVTAAGWQRNG
                     SATVAGTAVQLTPASAQAAGSVVYTS"
BASE COUNT         1252 a         2891 c         2749 g         1178 t
ORIGIN      
        1 gaggtggcgc gcatcgtccg gatgggcttc gagctggcgc ggacccggcg caagatcgtc
       61 acccaggtcg acaaggcgaa cgtgctggcc acctcacgcc tgtggcgccg ggtgaccgat
      121 gagatcgccg tcgagtaccc cgacgtgacc ctgggccacc agctcgtgga ctcgtgcgcg
      181 atgctgctga tccggcggcc ggccgagttc gacgtgatcg tcaccgagaa cctcttcggg
      241 gacatccttt ccgatgaggc cgccgtgctg gccggcagcc tcggcgtgct gccgtcggcc
      301 tcgctcggcg agcggcggac cgagcacggg acgttcggcc tgtacgagcc gatccatggc
      361 tcagcgccgg acatcgccgg cagcgacgtc gccgatccga tcgggaccat cctgtccgcg
      421 gcgatgctgc tgcggacgtc acttgggctc gaggcggcgg ccgcggccgt cgaggccgca
      481 gtcgatcgcg cgctcaccga cggctggcgg acggccgacc tggccgaccc gcgggacggg
      541 accgacggcc tcgtggtggt cggcacgacc gggttcacga ccgcggtgat cgaggcgctc
      601 gaggcggacg ccggggtggc cgcgtgagcg ctgcggccga cgcgcccgtg gtcctctacg
      661 acacgaccct gcgcgacggc acccagggcg agaacatcac gctctcgctc gccgacaagc
      721 tgcgcatcgc ccggatgctc gacgagttcg ggatgccctt catcgagggc ggctggccgg
      781 gctccaaccc caaggacatc gagttcttcg ccgccgcccg ctcgatgcgc tgggagcggg
      841 ccaagctcgc ggccttcggc tcgacccgcc accgcgcgaa caagcccgcc gacgacccga
      901 acctgcgcga gctggtcgcg gccgagaccc cggtcgtgac gatcttcggc aagagctggc
      961 tgctccatgt caccgaggtg ctgggcgcga gcccgaccga gaacctcgac atgatcgccg
     1021 agagcgttcg gctcatcgtc gacgagggcc gcgagctggt ctacgacgcc gagcacttct
     1081 tcgacggcta caaggcgaac caggcctacg cactctccac gctcaaggcc gcgcacgaga
     1141 gcggcgcgcg gaccctcgtc ctgtgcgaca cgaatggcgg gacgctgacg ggcgagctcc
     1201 tggccatcct cgacgcgacg cgggccgcga tcggcgccga tgccgtgacc tggggcattc
     1261 acacccacaa cgacgcggag ctggccgtcg cgaactccct cgccgccgtc caggcgggcg
     1321 tgcgccacgt ccaggccacg atcaacggct acggcgagcg caccggcaac gcgaacatgg
     1381 tcagcgttct cgccggcctc gcgctcaaga ccgaccttcc gctcgccccg gtcggcggcg
     1441 gcgatctctc cgcgctgacc gagctgtcgc gggccgtcgc ggagatcgcc aaccaggccc
     1501 cgaacgacta ccagccgtac gtcgggcgct cggcgttcgc gcacaagggc ggggtccacg
     1561 gcgccgcgtt tgcgaaggtc gaacgcagct accagcacat cgatccgacc gcggtcggca
     1621 acgaggggcg cctcgtcgtc tcggagctcg gcgggcgcgc caacacctcg atccgggcgc
     1681 ggcagctcgg ccaccagctg gacggggtga tcgaaccggc ggtcctctcg acgctcatca
     1741 agaagctcga gagcgagggc ctgtcgttcg aaggcgcgga ggcgtccttc gagctgctca
     1801 tccgacgcca ccaggcggac tacacgccgc ccttccgcgt cgtcgactac acgtgcctcg
     1861 tcgagcagcg ctccggcgcc gagcttcggg ccgaggcgac ggtcaaggtc gaggtcgacg
     1921 gtgagctgct gcacaccgca gccgagggca acggtccggt gaacgccctc gatgcggcgc
     1981 tgcgcaaggc gctgcgcgcg ttctacccgg tcctcgacac ggtccacctg tacgactaca
     2041 aggtccggat catcgactcc gggtcggcga cgggcgcgcg cacgcgggtc acgatcgaat
     2101 cgaccgacgg ttcggccgaa tggtcgacca tgggctcgga cacgaacatc atcgccgcct
     2161 cggcgaccgc cctcgcggac tcgctcgaat acgcaatctg gaagcgcggc gccgagctcc
     2221 ggcgccggga cgaacgacac ttcaccacgg tgatcgccgg ggaaggagcc tcatgagcgc
     2281 agcggacgca gccaccacgg cgagccaggc cctcgagctc acgcgctgga ccgtgacctc
     2341 gggcagcaac gtgcagagcc gcggcgccgt ggtcatcgac gccggcaacc accactggcg
     2401 cgcctcggcc gagggcaacg gcgcggtcga cgcgctcttc aacgcggtcg acgcggcact
     2461 tgccgaggtc ctcgacggcc ggccgcgcct cgttggctac gacgtgcacg cgctgggcca
     2521 gggacacgag gccgaagggc tggtcatggt cgcggtcgag ccgccggtcg ggctcgaggg
     2581 cggccgctcc ggtggcctgt tccagggcac ggctcgcagc acgaacatcg tcgcgtcgtc
     2641 gatcgaggcc tacatcgagg cgctcaactc gatgctcgcc gagcccgcct gggcgggcgc
     2701 cgccgaatct gccgccaagg accgccggaa aggccccgag acgcacggcc gccgcgggga
     2761 gatcgaccgc tcagcgaagg acgacaccgc gccgtggttt gatcgctaac tcgcgagcgg
     2821 tgaagagcat ctcggtcgcg tattcggggg agcccggggc attcgccgag gacgccgtcc
     2881 tggcctggtt cgcagcgccg gagccggtgc ccgtccaatc cttccgggcg gtgttcgagg
     2941 ccgttcggga cgggtcggcg cccgccggcg ttattcccgt cgagagctcg ctgctcggga
     3001 cgattcgcga gacgctcgac ctgctgtacg agttcgacct ggccatcgtc ggtgaggtca
     3061 gcgtgccggt tcgcctcgcc ctgctcgccc tcccgggtga gcggctggac accatccgca
     3121 cggtccagtc gatcgccgcg gcgctcgccc aggctgacga attcctgcgg tccggcagct
     3181 ggacggtcga gacgacgtgg aataccgcag gcggggcgcg cgcgatcgcg gaccgccggc
     3241 agcgcggcgc agccgcggtc gcctcgccac gggcggccgc cctctacggc ctcgaggtga
     3301 tcgccgacga catccagacc ggctcggaca accggacgcg gttcgcggtc atctgtcggc
     3361 acgcggcggg cctcgacgtc caggccgcgg ccgcggccgc agccatcgtg ggcgcgccga
     3421 agaccacgct ggtgttcggg gtcggcaacg tgcccggttc gctgcatcgg tcgctcggcg
     3481 cctttgccgg gcgcgggatc aacctgtcgc gcctcgagtc gcggccgtgg accggacgag
     3541 gatcccggtg ggagtacctg ttctgggcgg acctcgacgc ggacccgacc gcacctgcct
     3601 gcgcggcggc gctggccgag ctccgcgccg aggccgagat ggtgcgagtg ctcggcacgt
     3661 atccccgggc cgccgaggac tgaaagcgca gtccgcggcg caaagcacgg atcgccgccg
     3721 cagccgatga tggtgggcca catcgacgac cagtgaggtg atcgtggacg aggccttccg
     3781 caccgagatg gccgacggct acaagctcga cgagcctgcg ctcaccctcg gctcgccgct
     3841 cctcggcgac gaggtcctgc ccgacgtccg cgtgcagatc gcgctctccc ggatcaaccg
     3901 ccacggcctc gtcgcgggcg cgacggggac gggcaagacg aagaccctcc agatcctcgc
     3961 cgggcagctg tcggcgctcg gcgttcccgt gttcgccgtc gacgtcaagg gcgacgtctc
     4021 ggggatcggc gcgcccggcg acccggccga tcccaaggtc acggcgcggt gcgcgtcgat
     4081 cggctggaac ttcgtcgccg gtgggcatcc gctcgaggtc ctgtcactct cgggtaacaa
     4141 gggcgcgcac gttcgggcga gcgtctcgtc gttcggaccg ctgctcctgg gaaaggtcct
     4201 cgacctcaac gacacccaga cctcgatcct gtcgctcgtc ttccgctact gcgacgacca
     4261 gcacctgccg ctgctggatc tcgcggacct ccgcacgacg ctcaagttcc tcgccggcga
     4321 cgacggcaag gcggtcctgg aggacctggg cgggatcagc tcggcttcgc tcggggtcat
     4381 cctgcgtgcg atcgtgacga tcgagcagga gggcgctgac atcttcttcg gcgagcccga
     4441 gttcgacgtc aacgacctcc tccgaaccac cctcgacggg cgcgggatgg tcacgctcct
     4501 cgaggtcgcg gacgtgatgg accggccccg gctctactcg accttcgtcc tgtggatgct
     4561 ggcgcagctg tacgagacgc tgcccgagat cggcgacgtg cccaagccga agctcgcgtt
     4621 cttcttcgac gaggcgcacc tgctgttcaa ggacgcgtcc gaggcgctgc tcgagcagat
     4681 cgagcgaacc gcgcgcctga tccggtcgaa gggtgtcggc gtgtacttcg tcacccaggc
     4741 cccgaccgac gtgccttcat ccgtgctctc gcagctcggc aaccggatcc agcacgcatt
     4801 gcgcgccttc acccccgatg acgcggacgc gctccgcaag gcgtccagga ccttccccgt
     4861 cacgacccac tacgacgtcg agcgtacgat cacgtcgctc gggatcggcg aggcgctggt
     4921 gaccgtgctg tcgccgaagg gcgtgccgac gcccctggcc gcgacccgcc tcatcccgcc
     4981 cgactcgcgc atggccccgc tgaccgacca ggagctgtcg cagctcgtcg gcgcatcaca
     5041 gctccaggcc cggtacggga cgacggtcga tcgggagagc gcccacgaga tcatcacggc
     5101 gcgactcgcc gcggcccacc aggccgccgc cgcagcggcc ggggtgcccg tggcgccgcc
     5161 gggatcggac ccgggcgctc cgtacggcgg catgacgccg gcgcaatacc ggcgcgagat
     5221 cgagcgccag gcgcgcgagc aggtcaaaca gcaggaacgg gagcgcaagg ccgccgagcg
     5281 cgagcggaac gcgcaggaac gtgaacgcaa ggcagccgag cgcgagcgcc agcggacgat
     5341 caacaccggg gtgcggacgg cagggcgagt gatcacatcg cgcgtcgggc agtcactcat
     5401 ccggggcgtg ttcgacacga tcttcggcgg caagcgctag ccgttccttg aactgcccga
     5461 tgacgggtag tctgaaagcg ttgcgcgggg gcaccaacgg aagaatctgt gggttctcgg
     5521 tgtgcgggtt cggcatacta acgtcgacgc tcaggcgaca ccgaccctgg cggtggacgc
     5581 ggaggaggag gcgacaggga tgcccgcggt ccggtcaagc acgcgtacgg cctcgacctt
     5641 cgtcgtcgcc gccgtcctcg cgtggctcac gtttcccccg ctggtcccga gtacacgggc
     5701 cgctgacatc gcgccttcgg ccgatgccgc attggccagt ttggcggacc tcccgtcgaa
     5761 tttcgtcgac gagctcgtct tctcgggggt caccaacccg accaatatcg agttcgccac
     5821 ggacgggcgc gtattcgtag ccgaaaagag cggtcgtgtc ctcgtgttcg acaatctctc
     5881 cgacacgacg gcaaccgtct tcgccgacct ccggacgcag acccacaact actgggaccg
     5941 gggcctcctg ggactggcgc tccaccccaa cttccctgcg acgccctggg tctacgtctc
     6001 ctatacgtac gacgccgcca tcggtggcac cgcccctcgc tggggcactg caggacagac
     6061 tggggatccg tgcccgaacc cgccgggcgc gacgaccgac ggctgcgtgg tgtccagccg
     6121 ggtctcgcgt ctcacggcga acggcaatgc gtccatcggc gagaccgtcc tcgtcgagga
     6181 ttggtgtcag cagttcccga gccattcgat cggcgatctc gagttcgggc ctgacggtgc
     6241 cctgtacgtc agcggtggcg acggcgccga cttcaacgtg gtcgactttg gccagaaggg
     6301 gagtccgctg aacccctgcg gggacccacc ctcgggtgtc ggtggtgtgc agacgtcacc
     6361 gagtgccgag ggaggggcgc tccgctcgca ggatctgcgc acgagcgggg accccgtggg
     6421 gttggccggc tcgatcctcc ggatcgaccc cgacacgggc gctggcttgc ccggcaaccc
     6481 cctgttcggc aacgcggatc cgaacgcccg gcgaattatt gccgagggcc tgcggaaccc
     6541 gttccggttc accttccgcc cggggacgga cgagctctgg ctcggcgacg tcggctggaa
     6601 tacgtgggag gaggtcgacc gcatcaccga tacgaccccg aacgatccgg tgaacttcgg
     6661 ctggccgtgc tacgagggga acgggcgcca gcccggctat gacgcggcga acctgacggt
     6721 ttgcgagacg ctctacggcg catcgggcgc cgtccagtcg ccgttcttca cctacagcca
     6781 ctcgctcgat atcgccccgc ccgacgactg cccgccggcc gcgccgccgg ctccggtcag
     6841 ctcgtcagtc actgggctgg ccttctacac agggacggct tttccggcca tttatagcgg
     6901 agcgttgttc ggggccgact actcgcggaa ctgcatctgg gtcatgcggg ccgacgggaa
     6961 cggtgttccc gacccgtcga cgatccaggg tttccagccc gcggcccagg ggcccgtcgt
     7021 gctcaagatg ggtcctgacg gatcgctcta ttacgcgaac ttcaacggcg ggatccggcg
     7081 aattcggtac accgtcaacc tgccgccgac cgccaacatg acggcctcgc ccctgggcgg
     7141 acctgcgccc ctcgacgtct cgttcgacgg gacgacctcg accgaccccg agggccacgc
     7201 gctcagctac gcgtgggacc tggacgacga cggcaccttc aacgacggca cgggttctac
     7261 cgccgcgttc acctacacca ccaccgggca gcatcgagcc cggctgcgcg tgaccgaccc
     7321 aggcggggaa gtcgataccg agcaggtctt catccaggtg gacaactcgc tgcctgcgat
     7381 ctcgatcacg gcgccatcag cctcgctcca atggtcggtc ggcgaagaca tcgcatttgc
     7441 cggaactgct accgatgcgc aagacggtga catctcgaac cagatcgact gggatctcac
     7501 gctcttccac tgccaggggg tgctctgcca cacccacgca atcacgagct ggcaggacac
     7561 ggcaggcgcc acgttcagcg cgccagatca cgagtacccg tcgtacctcg agctcaaggc
     7621 caccgtcacg gactcgagcg gcgcgactgt gtctgtgaca agacgcatcg acccgaagac
     7681 cgtcgcgctc acgttcctga cgtcaccggc gggcctgacg gtgactgtcg gtggaaccag
     7741 cccacaggct gctccgttca cgaggacgat gatcgtcggg tcggacaata cggtcgcggt
     7801 ggagtcgccg cagctgaccg ggggtgggac ctcttacacc tttgcgtcct ggtccgatgg
     7861 cggggggctg tcccactcga tcacggctcc cgcgaccgcg acctcgtaca ccgcgaactt
     7921 cacgcccacc gcccccagca gctgtcccga cacgctgccc gtggtgaccg ctgcgggctg
     7981 gcagcgcaac ggctcggcga cggtggcggg cacggcggtc cagctgaccc cggcctcggc
     8041 gcaagcggcc ggcagcgtcg tctacacctc
//
LOCUS       DATBSW010000038         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_955372, whole genome shotgun sequence.
ACCESSION   DATBSW010000038 DATBSW010000000
VERSION     DATBSW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955372"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VIF63_01065"
     CDS             complement(<1..599)
                     /locus_tag="VIF63_01065"
                     /inference="COORDINATES: protein motif:HMM:NF027966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8237997.1"
                     /translation="MNDDLPPLPRFLHGVASGDPTPRKVVIWTRVSGASAAESVPVRW
                     RILEADGERPAGHGRSLARPDSDWTVRIDIGGLRPDTHYLYDFEVDGERSPQGRTRTL
                     PEGDPASIRFGQVSCAKYNAGNFNAYARLAERDDVAFVLHLGDWIYEASQTPPASQTP
                     SKDIGRPFEPLEECKTLGDYRMRYAQYRRDPDVQAVSAA"
     gene            complement(626..2029)
                     /locus_tag="VIF63_01070"
     CDS             complement(626..2029)
                     /locus_tag="VIF63_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEY8237998.1"
                     /translation="MNGLERREQPAKRVIIIGAGMAGLVAGYELARAGHDPLILEAQN
                     RVGGRVYTLRKFAPGLYAEAGAMRIPRVHDLTLGYIEHFGLELRPFMMGNPKGLVHVG
                     GKRMTAEQADLTPDELGFAVADHERGRSAFALWEEATKDLKAMLEADAVTGWEYIVRE
                     FDGYTLREFLEARGFSEGAIEMYGVMNFVEAGINNAVTEEFREDFGNAYEDMQEIIGG
                     TDRLPTAFYGELADRIRFGAEVHAIDQDTDSVTVHFKTEAGRYSVSGDYAIVTLPFSV
                     LRQVEVLTPFSREKQRAIRQLNYSASTKVLFQVRERIWETDDGIYGGATVTDLPIRRM
                     NYPTPDPTTSRGMLLASYTWDQDAARWGAMDEETRLEEALDDVAKIHPRIREVYEVGA
                     SHAWYDDRFANGAFALFEAGQQTQLQADIVKPEGRIHFAGEHCSLYHAWIQGALESGL
                     RAARAIHEAPAIEAASA"
     gene            complement(2088..>2438)
                     /locus_tag="VIF63_01075"
     CDS             complement(2088..>2438)
                     /locus_tag="VIF63_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8237999.1"
                     /translation="SAGPHDLFNAVIAEPNLDSFIMQLAWSGAVDDENFTELRTAEWL
                     VTFQFGDLRKNVRLSEFGLADSQIVTAGLYHGPQRVTFTGTYHDNEQDLPVNGVVACE
                     GDLRQQTQSQPPSS"
BASE COUNT          351 a          831 c          849 g          407 t
ORIGIN      
        1 tgcgcggcac tgaccgcctg gacgtcgggg tcgcgccgat attgtgcgta gcgcatccgg
       61 tagtcgccga gggtcttgca ttcctcgagc ggctcgaacg gccgcccgat gtccttgctc
      121 ggtgtctggc tcgccggcgg cgtctgcgag gcctcgtaga tccagtcccc gaggtggagc
      181 acgaacgcca cgtcgtcgcg ctcggcgagc cgggcatagg cgttgaagtt gccggcgttg
      241 tacttggcgc acgacacctg cccgaaccgg atcgaggccg ggtcgccctc tggcagcgtg
      301 cgcgtccggc cctggggcga ccgttcgccg tcgacctcga agtcgtacag gtagtgcgtg
      361 tcgggccgca ggccgccgat gtcgatgcgc acggtccagt cggaatcggg ccgcgccagg
      421 gacctgccgt ggcccgccgg ccgctcgccg tcggcctcga ggattcgcca gcgcacgggc
      481 acggattccg cggccgacgc gccgctgacg cgcgtccaga tgacgacctt gcgcggtgtt
      541 gggtcgccgc tggcgacgcc gtgcaggaag cgtgggagcg gcggcaggtc gtcgttcacg
      601 acgggctcgc cacgaaccgc gccgatcagg ccgatgctgc ttcgatcgcg ggcgcctcgt
      661 ggatcgccct ggcagcgcgc agcccggact ccaacgctcc ctggatccac gcgtggtaca
      721 gcgagcagtg ctcgccggcg aagtggatcc ggccctcggg cttcacgatg tccgcctgga
      781 gctgggtctg ctggcccgcc tcgaacaggg cgaaggcgcc gttggcgaac cggtcgtcgt
      841 accacgcgtg ggacgccccg acctcgtaca cctcgcggat gcgcgggtga atcttggcga
      901 cgtcgtcgag cgcctcctcg agccgcgtct cctcgtccat cgcgccccac cgagccgcgt
      961 cctggtccca cgtgtagctc gcgaggagca tgccccggga ggtggtcggg tccggtgtcg
     1021 ggtagttcat ccggcggatc ggcaggtcgg tgacggtcgc accgccgtaa atgccgtcat
     1081 cggtctccca gatccgttcc cggacctgga acagcacctt cgtggacgcc gagtagttga
     1141 gctggcggat cgcgcgctgc ttctcgcgcg agaacggcgt cagcacctcg acctgccgga
     1201 ggaccgagaa cgggagcgtg acgatcgcgt agtcgccgct gaccgaatac cgcccggcct
     1261 cggtcttgaa gtggacggtg accgagtccg tgtcctggtc gatcgcgtgg acctcggcgc
     1321 cgaaccggat ccgatcggca agctcgccgt agaacgcggt cggcaggcga tccgtcccgc
     1381 cgatgatctc ctgcatgtcc tcgtaggcgt tgccgaaatc ctcccggaac tcctcggtga
     1441 cggcgttgtt gatgccggcc tcgacgaagt tcatgacgcc gtacatctcg atggcgccct
     1501 cggagaagcc cctggcctcg aggaactccc ggagcgtgta cccgtcgaac tcgcggacga
     1561 tgtactccca gccggtcacc gcatcggcct cgagcatggc cttgaggtcc ttcgtcgcct
     1621 cctcccagag cgcgaacgcc gatcgcccgc gctcgtggtc ggcgacggca aagcccagct
     1681 catcgggcgt gaggtcggcc tgctcggcgg tcatccgctt gccgccgacg tgcacgaggc
     1741 ccttggggtt gcccatcatg aacgggcgca gctccagccc gaagtgctcg atgtagccga
     1801 gggtcaggtc gtggacgcgc gggatgcgca tcgcgcccgc ctcggcatac aggcccggcg
     1861 cgaacttgcg gagggtgtag acgcggccgc ccacgcggtt ctgtgcctcg aggatcagcg
     1921 ggtcgtggcc ggcccgggcg agctcatagc cggcgacgag gccggccatc ccggcaccga
     1981 tgatgatcac ccgcttggcg ggctgctcgc gacgctcaag tccgttcatc ggtcgaatct
     2041 agcggcgcag gcgcgacgcg atggcgtcgc cgcgcacgcg ccgatgctca gctgctcggt
     2101 ggctgcgact gcgtctgctg gcggagatcg ccttcgcaag cgaccacgcc gttgacgggg
     2161 aggtcctgtt cgttgtcgtg atacgtgccg gtgaaggtca cccgctgcgg cccgtggtac
     2221 agacccgcgg tcacgatctg cgaatcggcc agaccgaact ccgagaggcg cacgttcttt
     2281 cgcagatcgc cgaactggaa ggtgaccagc cactccgccg tgcgcagctc cgtgaagttc
     2341 tcgtcgtcca cggcgcccga ccaggccaat tgcatgatga acgagtcgag gttgggctcg
     2401 gcaatgaccg cgttgaacaa gtcgtgggga cctgcgga
//
LOCUS       DATBSW010000039         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_73694, whole genome shotgun sequence.
ACCESSION   DATBSW010000039 DATBSW010000000
VERSION     DATBSW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73694"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /gene="mraY"
                     /locus_tag="VIF63_01080"
     CDS             <1..779
                     /gene="mraY"
                     /locus_tag="VIF63_01080"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8238000.1"
                     /translation="DFKLPPGGIIAPLATLLLVGGLGAFDDYLNAKTGEGISARQKML
                     WLVVVAVAAAYQIQDTYAIDRIAVPFIGFVMIDPWLYIAFAAFAIIAAANGVNLTDGL
                     DGLAGGTLVFAFVAFVLVALLNQPTQPNLAVLCGLIVGGLLGFLWFNVHPAQVFMGDS
                     GSLALGATLAVTALITSQILILPVIGLVFVIETLSVIIQVGYFKLTKGKRIFRMSPLH
                     NHYELLGWDEEKITLRFWIVGIIAGLIGVTLFLASINQLQ"
     gene            785..>1506
                     /locus_tag="VIF63_01085"
     CDS             785..>1506
                     /locus_tag="VIF63_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY8238001.1"
                     /translation="MTAGAVIDPAELAPGALADGVFRGRAVSVLGFARSGIALARFLA
                     DAGAEVTVYDARPADDLGKAIAGLEGRSVRLLLGPDVDPAEAVAGQALVTTSPSINPD
                     YPTTEPRLRAVLTDLVARRAAGDLAAPGIVSEVDLFLRLCPAPTIGITGTKGKTTTSA
                     LTHALLKAHLGDRAVLGGNIGIPLIEQLPRLTPGHVVVVELSELQLPTLSRGTTVAVY
                     TNVTSDHLDRHGSLEAYRRVKQR"
BASE COUNT          209 a          550 c          492 g          255 t
ORIGIN      
        1 acgacttcaa gctccccccg ggcggcatca tcgccccgct cgcgacgctg ctcctggtcg
       61 gtgggctggg cgccttcgac gactacctca acgccaagac cggcgagggc atcagcgccc
      121 gccagaagat gctgtggctc gtggtcgtcg cggtcgccgc ggcgtaccag atccaggaca
      181 cgtacgcgat cgaccggatc gccgtgccgt tcatcggctt cgtgatgatc gacccttggc
      241 tgtacatcgc cttcgccgca ttcgcgatca tcgcggcagc gaacggcgtg aacctcaccg
      301 acggcctcga cggcctcgcc gggggcacgc tggtgttcgc cttcgtggcg ttcgtcctcg
      361 tggccctcct caaccagccg acccagccga acctcgcggt cctgtgtggc ctgatcgtcg
      421 gcggcttgct tgggttcctg tggttcaacg tccacccggc ccaggtgttc atgggcgact
      481 cgggatcgct ggccctcggc gcgacgctcg cggtcacggc cctgatcacg agccagatcc
      541 tgatcctgcc ggtgatcggc ctcgtgttcg tgatcgagac cctgtcggtg atcatccagg
      601 tcggctactt caagctcacg aagggcaagc gcatcttccg gatgagcccg ctccacaacc
      661 actacgagct cctcggctgg gacgaggaga agatcacgct ccgcttctgg atcgtgggga
      721 tcatcgccgg actcatcggc gtgaccctgt tcctcgcctc gatcaaccag ctgcagtgag
      781 cgtcatgacc gccggcgccg tcatcgatcc cgcggagctc gcgccgggcg cgctggcgga
      841 cggcgtcttc cgcgggcgcg cggtcagcgt gctcgggttc gcgcggtccg ggatcgccct
      901 tgctcgtttc ctcgccgatg ccggggcgga ggtcaccgtc tacgacgctc gaccggccga
      961 cgacctcggc aaggcgatcg caggactcga gggccgatcc gtccgactgc tcctcgggcc
     1021 ggacgtggat ccggcggagg ccgtcgccgg ccaggcgctc gtgacgacgt cgccgtcgat
     1081 caatcccgac tacccgacga cggagccgcg gctccgcgcc gtgctgaccg acctcgtcgc
     1141 tcgccgcgcc gccggcgacc tcgccgcacc ggggatcgtg tccgaggtcg acctgttcct
     1201 ccggctgtgc ccggcgccga ccatcgggat caccggcacg aagggcaaga cgacgacctc
     1261 ggcgctcacc catgcgctgc tcaaggccca cctcggtgac cgcgcggtcc tcggcggcaa
     1321 tatcgggatc ccgctgatcg agcagctgcc gcggttgacg cccggccacg tcgtggtggt
     1381 cgagctgtcc gagctgcagc tgccgacgct gtcgcgtggc acgaccgtcg cggtctacac
     1441 gaacgtcacg tccgaccacc tcgatcggca cgggtcgctc gaagcctacc ggcgggtgaa
     1501 gcagcg
//
LOCUS       DATBSW010000040         4832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_391180, whole genome shotgun sequence.
ACCESSION   DATBSW010000040 DATBSW010000000
VERSION     DATBSW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391180"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VIF63_01090"
     CDS             complement(<1..996)
                     /locus_tag="VIF63_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8238002.1"
                     /translation="MSPAADGDRQERKKLLVAPHRRTGNRNFRPAEKAAIWGYRAGTW
                     LMSRLPVPVARGIVSFALQVSFVLWPKKRRYVNDNFAHVLGKPPGSLEVKRKALAAYR
                     SYARYVVELMRLPRMTNEQAAALVDTSTLLPLEAEWKASGKGLIITSAHIGNLEGVAR
                     GIARHGWPIASLGDDTTFPELFDMLRRQRRDWGINLIPWRNLRDMFGVLRRNEILALI
                     IDWGYRPEDIPVRLFGAWTTLPAGPAALAAKTGAPIVHCAVRRSPDGRTFQLTLSEPI
                     IVTSSDPVELQRATQAIADALGETIAAAPEQWYSFKPIWPATAEEQAVLEARAASG"
     gene            complement(993..2270)
                     /locus_tag="VIF63_01095"
     CDS             complement(993..2270)
                     /locus_tag="VIF63_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY8238003.1"
                     /translation="MASNGKNGKSKNGAHPENTWAGAGRRGDGKVRVAIVGVGNCASS
                     LVQGRYYYENAKEDDFVPGLMHVNLGGYHVRDIDFVAAFDIDKNKVGKDLSEAIYTKP
                     NNTFVFQKVPHTGVTVERGMTHDGLGKYLSQVIEKAPGPTADIVSILKEREVDVMVSY
                     LPVGSEEATKWYVEQALRAGVAFVNAIPVFIGREPYWQRRFAEEGLPVIGDDIKSQVG
                     ATIAHRVLTRLFMDRGVRLDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMLD
                     YELEDGDVHVGPSDHVPWLLDRKWCYIRMEGTTFGDVPLNAELKLEVWDSPNSAGVIT
                     DAIRCLKLGLDRGLKGTLVAPSSYFMKSPPQQIHDDIAFNRVEAFIKGEDNETLVGTE
                     APVKRKLRKMAASGPGAAQLAASGARAKAAAGA"
     gene            complement(2412..3131)
                     /locus_tag="VIF63_01100"
     CDS             complement(2412..3131)
                     /locus_tag="VIF63_01100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY8238004.1"
                     /translation="MTADRPGAASASLVSPALRDRVRGLAAPIARGLGRLGLSPNALT
                     LIGFVGTCLGALAAANGRWLIAGLLILGFGIFDLFDGTLARQTGRATKLGAFLDSTFD
                     RAGEGIVYVGIAAGSLGGGFSPGAILAASAMAAAFMVSYTRAKSESLGFASGTGMASV
                     GLAPREVRIAILSIGLIWLGLIPTGLACDLVDPGTPTSCFAGGSLGETVLLGTLSLIT
                     VLATITTIQRILHVRAQSREG"
     gene            complement(3128..3484)
                     /locus_tag="VIF63_01105"
     CDS             complement(3128..3484)
                     /locus_tag="VIF63_01105"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8238005.1"
                     /translation="MAPSPAHIRSTIAAVVDQPDELRALRIVHKALADVNRLRIVQRL
                     AQGEATVSELIDRIGLSQPLVSWHLGRLRVAGLIQTRRVGRETVSRLVPEAFHAFVEY
                     ERRILGIRGEQEQARP"
     gene            complement(3605..4279)
                     /locus_tag="VIF63_01110"
     CDS             complement(3605..4279)
                     /locus_tag="VIF63_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238006.1"
                     /translation="MTLRARRRRRWGIGLIAFGAIGLVLIIAAGVLVLGSFAVLDDAA
                     SGFERQRVQVVALLEPAATALDHAATSAANAGASLGATRDAAARAATLTARLAESFEG
                     LAALSSFEVLGARPFGTLAGQFAAVGSEARLVSTDLATAADTMGTNVSDSQAVASDLR
                     ALATRLQTLRAGLGVSSGTDTSSGTGSLQIDTARLVLLGLLAWLAVPALVSLWFGARL
                     LRVRPG"
     gene            complement(4276..>4832)
                     /locus_tag="VIF63_01115"
     CDS             complement(4276..>4832)
                     /locus_tag="VIF63_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238007.1"
                     /translation="QHMAVILIALSIVRERNSGAIDRFRSSPMRATEVVIGKVLAFAL
                     LGGAISAISVWLLVTLLGVPMVGSAAGVALVLGLLLFASLGLGLLISVVSDSERQAVQ
                     LALLTLLASMFFSGFVLRIEEFQPAVQVLAYLLPVTHGIALLQETMLTGSIGATWQLV
                     ALGGIGAVLVSLSWLLLRRELRPE"
BASE COUNT          750 a         1602 c         1767 g          713 t
ORIGIN      
        1 gccgctcgcc gcccgcgcct cgagcacggc ctgctcctcg gcggtcgccg gccagatcgg
       61 cttgaagctg taccactgct cgggcgccgc ggcgatcgtc tcgcccagcg cgtcggcaat
      121 ggcctgcgtt gctcgctgga gctccaccgg atcggacgag gtgacgatga tcggctcgct
      181 gagggtgagc tggaacgtcc ggccatcggg cgagcgacgg acggcgcagt gcacgatcgg
      241 cgcgccggtc ttcgcggcaa gcgccgccgg tcccgcaggc agcgtggtcc aggcgccgaa
      301 cagccgcacc gggatgtctt ccggccggta gccccagtcg atgatcaggg ccaggatctc
      361 gttccggcgg agcacgccga acatgtcccg caggttgcgc cacgggatca ggttgatgcc
      421 ccagtcgcgg cgctggcgcc ggagcatgtc gaacagctcg gggaacgtcg tgtcgtcacc
      481 gagcgacgcg atcggccagc cgtggcgggc gatgccccgc gcgacgccct cgaggttgcc
      541 gatgtgcgcg ctcgtgatga tcaggccctt gccgctcgcc ttccactccg cttccaaggg
      601 gagcagcgtg gacgtgtcga caagcgccgc cgcctgctcg tttgtcatcc gcggcaggcg
      661 catcagctcg acgacgtacc gcgcatacga gcggtacgcc gcgagggcct tgcgcttgac
      721 ctcgaggctg cccgggggct tgccgaggac gtgcgcgaag ttgtcgttca cgtagcggcg
      781 cttcttcggc cacagcacga acgaaacctg gagggcgaag ctgacgatcc cgcgggcgac
      841 cggcacgggc aggcggctca tcagccacgt gcccgcgcgg tagccccaga tcgcggcctt
      901 ctcggcgggg cggaagttgc gattgccggt gcgccggtgg ggcgcgacga gcagcttctt
      961 ccgctcctgc cgatcgccgt cggcagccgg gctcacgcgc cggccgccgc ctttgcgcga
     1021 gcgcccgacg ctgcgagctg cgcggcaccc gggccgctcg cggccatctt gcggagcttg
     1081 cgcttgaccg gcgcctcggt cccgacgagc gtctcgttgt cctcgccctt gatgaaggct
     1141 tcgacccggt tgaacgcgat gtcgtcgtgg atctgctgcg gcgggctctt catgaagtag
     1201 ctggagggcg cgacgagcgt gcccttcagg cctcgatcca ggccgagctt caggcagcgg
     1261 atcgcgtcgg tgatgacgcc ggcgctgttc gggctgtccc agacctcgag cttgagctcg
     1321 gcgttgagcg ggacgtcgcc gaaggtcgtg ccctccatcc ggatgtagca ccacttgcgg
     1381 tcgagcagcc acggcacgtg gtcgctcggg ccgacgtgga cgtcgccgtc ctcgagttcg
     1441 tagtcgagca tcgacgtgac cgcgttcgtc ttcgagatct tcttggactc gaggcgctcg
     1501 cgctcgagca tgttcaggaa gtcggtgttg ccgccgaagt tgagctggta cgtgcgatcg
     1561 aggcggacgc cgcgatccat gaacaggcgg gtcagcacgc ggtgggcgat cgtggccccg
     1621 acctggctct tgatgtcgtc gccgatgacc gggaggcctt cctcggcgaa ccggcgctgc
     1681 cagtacggct cacgtccgat gaacaccggg atggcgttca cgaacgcgac gccggcgcgg
     1741 agcgcctgct cgacgtacca cttggtcgcc tcctccgagc cgaccggcag gtacgagacc
     1801 atgacgtcga cttcgcgctc cttgaggatg ctgacgatgt cggcggtcgg gccgggggcc
     1861 ttctcgatga cctggctgag gtacttgccg aggccgtcgt gggtcatgcc ccgctcgacg
     1921 gtgacgcccg tgtgcgggac cttctggaag acgaacgtgt tgttcggctt ggtgtagatc
     1981 gcctcggaca ggtccttgcc gaccttgttc ttgtcgatgt cgaaggcggc cacgaagtcg
     2041 atgtcgcgga cgtggtagcc gccgaggttg acgtgcatca gcccggggac gaagtcgtcc
     2101 tccttggcgt tctcgtagta gtagcggccc tggacgaggc tgctcgcgca gttgcccacg
     2161 ccgacgatcg cgacgcggac cttgccgtca ccgcggcgcc cggcgccggc ccacgtgttc
     2221 tcggggtggg cgccgttctt gctcttgccg ttcttgccgt tgctggccat gtggcgtgct
     2281 ccttgctgtt ggttcgtatg cgcggattgg gttatcaggt tgccgttgcc cttggcgatg
     2341 ccctaaccgg catacatacg aaggcgacgg ggtggaagtg gccctccggc gagggcgtgg
     2401 gcggcgccgg ctcagccctc cctcgactgg gcgcgaacat ggaggatgcg ctggatggtg
     2461 gtgatggtcg cgaggaccgt gatcagggaa agggttccga ggaggaccgt ctcgccgagc
     2521 gagcccccgg cgaagcacga cgtcggagtg cctggatcga cgaggtcgca ggcgaggccg
     2581 gtcgggatca ggccaagcca gatcaggccg atcgacagga tcgcgatccg aacctctcgt
     2641 ggcgcgagcc cgacgctcgc catgcccgtc ccggaggcga agccgaggct ttcggacttc
     2701 gcgcgggtgt agctgaccat gaaggcggca gccatcgccg acgccgcgag aatcgcgccc
     2761 gggctgaagc cgcccccgag agaaccggcc gcgatgccca cgtacacgat tccctcgccg
     2821 gcccgatcga acgtggagtc caggaacgcg ccgagcttcg tggcacgacc cgtttggcgg
     2881 gccagcgtgc cgtcgaagag gtcgaagatg ccgaacccga gaatcaggag cccggcgatc
     2941 agccagcggc cattcgcggc ggcgagcgcg cccaggcacg tgccgacgaa gccgatcagg
     3001 gtcagcgcgt tcgggctgag gccgaggcgg ccgaggccgc gggcgatcgg cgcggcgagg
     3061 ccgcggacgc ggtcgcgcag cgcgggcgag acgagcgagg cgctggcggc gccggggcgg
     3121 tccgcggtca cgggcgggcc tgctcctgct ccccgcggat gccgaggatg cggcgctcgt
     3181 actcgacgaa ggcgtggaac gcctcgggta cgaggcgaga gaccgtctcg cgacccacgc
     3241 gccgggtctg gatgaggccc gcgacgcgga gccggcccag atgccacgag acgagcggct
     3301 ggctcagccc gatgcggtcg atgagctcgg acacggtggc ctcgccctgg gcgaggcgct
     3361 ggacgatccg cagccggttc acgtcggcga gggccttgtg gacgatgcgc agcgcgcgca
     3421 gctcgtcggg ctggtcgacg acggccgcga tggtcgagcg gatgtgtgca ggggacgggg
     3481 ccaagactgg tgctccgatc gggggacggg tgagaaaggc gccggctggc ggccgacgga
     3541 tcaacggtgg tttatatcaa tatgcattga tccggcgggc gtgtcaaccg ggaatatcgc
     3601 cctgtcagcc ggggcgcact cgcagcaggc gggcgccgaa ccagaggctc acgagggccg
     3661 gcacggcaag ccaggcgagc agcccgagca gcacgaggcg ggcggtgtcg atctgcaggc
     3721 tgccggtccc agacgaggta tctgtcccgg acgagacgcc gagcccggca cgaagcgttt
     3781 ggaggcgggt ggcgagcgcg cgcaggtccg acgcgacggc ctggctgtcg ctcacgttgg
     3841 tgcccatcgt gtcggccgcg gttgccaggt cggtcgagac gagtcgcgcc tcgctcccga
     3901 ccgcggcgaa ctggccggcg agcgtaccga acgggcgcgc gccgaggact tcgaatgacg
     3961 acagcgcagc gagaccttcg aacgactccg ccaatcgcgc cgtcagggtg gcggcccgtg
     4021 ctgccgcgtc cctggtcgca ccgaggctcg caccggcatt ggcggcgctc gtggctgcgt
     4081 ggtcgagcgc cgtcgcggcc ggctcgagga gcgccacgac ctgcacccgc tgacgctcga
     4141 agccggaggc cgcatcgtcg aggacggcga acgatccaag gacgagcacg ccggcggcga
     4201 tgatcagcac gaggccgatg gccccgaacg cgatcaggcc gatcccccag cgccgccgcc
     4261 gacgagcgcg cagtgtcact cgggccggag ctcccggcgg agcagcagcc agctcagcga
     4321 tacgaggaca gcgccgattc cgccgagcgc gacgagctgc catgtcgcgc cgatcgaccc
     4381 ggtcagcatc gtttcctgga gcagggcgat tccgtgcgtc acggggagaa ggtacgcgag
     4441 cacctgcacg gccggttgga actcctcgat gcgcaacacg aagccgctga agaacatcga
     4501 ggcgaggagc gtcaacagcg ccagctgcac cgcctgccgc tccgaatccg agacgaccga
     4561 gatcaacagg ccgagcccga gcgaggcgaa gagcagcaag ccgagcacga gcgcgacacc
     4621 ggcggcagaa ccgaccatcg gcacgccgag cagcgtcacg agcagccaca cggagatcgc
     4681 ggagatggcc ccaccgagca gcgcaaacgc gaggaccttg ccgatcacga cctcggtcgc
     4741 gcgcatcggc gacgaccgga atcgatcgat cgcgccgctg tttcgctcgc gcacgatgga
     4801 cagcgcgatc aggatgaccg ccatgtgctg aa
//
LOCUS       DATBSW010000041         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_285419, whole genome shotgun sequence.
ACCESSION   DATBSW010000041 DATBSW010000000
VERSION     DATBSW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285419"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VIF63_01120"
     CDS             complement(<1..297)
                     /locus_tag="VIF63_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8238008.1"
                     /translation="MRIAVLSDIHANLVALDAVLVDAGKIDAIWHLGDVVGYGPDPDG
                     VVARLREAGAIGVRGNHDAAAVGGSEIDWFNTDARRAMEWTRTAIGPEALEWLAA"
     gene            complement(652..972)
                     /locus_tag="VIF63_01125"
     CDS             complement(652..972)
                     /locus_tag="VIF63_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY8238009.1"
                     /translation="MDSADHFRHSYTRDKSQLVRRLARIEGQVRGITRMIEREEYCVD
                     ILQQTSALRAAVDSLAVLVLEDHVTGCVRTAAANGDADRYIEEVVDVVRRTLGRPVRA
                     APRS"
     gene            1060..1383
                     /locus_tag="VIF63_01130"
     CDS             1060..1383
                     /locus_tag="VIF63_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238010.1"
                     /translation="MDPLDHLLALPDGPRCTVCDERVPLDRVKLLAWRDDLCFLQLDC
                     GACLSTTLGFVLGGRGDEPTPASTAAPFTTDDVLDMHQLLAGWSGDLTGLLAHAERGR
                     AEPSR"
     gene            1380..2093
                     /locus_tag="VIF63_01135"
     CDS             1380..2093
                     /locus_tag="VIF63_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238011.1"
                     /translation="MSGGTREATGTPPSGFGPGIADAIAAEPVSADVPAAEATSPETS
                     AAERSAVDVTRFAHASEAEMARILDFYAVRWDYEPTTFPILWNLEGEVVESFAPDFYL
                     PDLEMFLEMTTLKQRLVRKKNRKLRRLRELYPDIRIKLFYARDFRAMLLKYGRLALAE
                     ALSGTPGQVVPERARVVVEPDAAIEVLAEVEEPEAADAPTVVIEAPIPRPRPRARPGR
                     RRRRSSSSKVAGAEREAGA"
     gene            2090..>2620
                     /gene="hpt"
                     /locus_tag="VIF63_01140"
     CDS             2090..>2620
                     /gene="hpt"
                     /locus_tag="VIF63_01140"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8238012.1"
                     /translation="MTTEVDQRADIGEVLLTEDQIQAKVAELGAQISSDLAGRQLTLV
                     SVLKGSLPFMADLMRAISLPLRIDLMEVSSYGGATTESSGLVRILKDLSSSIEGEDVL
                     IVEDIIDTGLTLNYLVRYLRGKNPASLRICTLLDKPARRLVEIEVDYTGFTIPDQFVV
                     GYGLDYGELYRNLRFVG"
BASE COUNT          385 a          945 c          905 g          385 t
ORIGIN      
        1 ggcagcgagc cattcgagcg cctcgggccc gatcgcggtt cgggtccatt ccatcgcgcg
       61 gcgggcatcg gtgttgaacc agtcgatctc ggagccgccg accgcggcag cgtcgtgatt
      121 gccgcggacc ccgatcgcgc cggcctcgcg caggcgggcg accacgccgt cagggtccgg
      181 gccgtacccg acgacgtcgc cgaggtgcca gatcgcgtcg atcttgcccg cgtcgacgag
      241 gaccgcgtcc aatgccacga gattggcgtg gatgtccgac aggacggcga tccgcatggg
      301 caggtcagcc tagcaagggg cccagacgcg cgtcggaggc ggcgctcggg cctccgccca
      361 ggctctcgtg tggagagacc acgggcaccc ggcgtaccgc ctccggcgtc gtcgttcatc
      421 gctgcctggg cgtcggtctg acgcgatccg ttcggttcat ggtcgggttt ccccggaccc
      481 ctcacgaagc gcgttccccg gagaatcccc ggcgcggatg caggatgttg cccgggccgg
      541 gtggtcgggt caacccggtt atccaccgat cgcaccggcc aacgaccgat ggtggataag
      601 tcccggcgtt gcccaccagg ggcggtgcct gggccttggc ccggcggagc gctagctccg
      661 cggcgcggcc cggaccggcc gaccgagcgt cctccggacg acgtcgacga cctcttcgat
      721 gtaccgatcg gcgtcgccgt tggccgcggc ggtccggacg cagccggtga cgtggtcctc
      781 gaggacgagc accgccagcg aatcaaccgc ggcgcgcagc gcgctggtct gctggaggat
      841 gtccacgcag tactcctcgc gctcgatcat gcgggtgatg ccgcgcacct ggccctcgat
      901 gcgggcgagc cggcgcacca gctgcgactt gtcgcgggtg tagctgtgac ggaagtgatc
      961 tgcgctgtcc atgcgttcga tgataccccc cgggggtatc gcggacaagg gatcggcgcc
     1021 gagggcgatc cggcggctca ccggctacac tcacctcgga tggatcccct ggaccacctc
     1081 ctcgcactcc cggacgggcc tcgctgcacc gtctgcgacg agcgcgtccc cctcgaccga
     1141 gtgaagctcc tcgcctggcg cgacgacctc tgcttcctcc agctcgattg cggcgcctgc
     1201 ctgagcacca ccctcggctt cgtgctcggc ggccgcggcg acgaaccgac gccggcctcg
     1261 acggccgcgc cgttcaccac ggacgacgtc ctcgacatgc accagctcct cgcgggctgg
     1321 tcgggcgatc tcaccgggct cctggcgcac gccgagcgcg gccgcgccga accgagccgg
     1381 tgagcggcgg gacgcgagag gcgacaggaa cgcctccatc ggggttcggg cccgggatcg
     1441 ccgacgcgat cgccgcggag ccggtgtccg ctgacgtgcc cgccgcggag gcgaccagcc
     1501 cagagacgtc cgcggccgag cggagcgccg tcgacgtcac ccgcttcgcg cacgccagcg
     1561 aagccgagat ggcgcggatc ctcgacttct acgccgtccg ctgggactac gagccgacga
     1621 ccttcccgat cctgtggaac ctcgagggcg aggtcgtgga gagcttcgcc ccggacttct
     1681 acctgcccga cctcgagatg ttcctcgaga tgacgaccct caagcagcgc ctcgtgcgca
     1741 agaagaatcg caagctgcga cggctgcgcg agctgtaccc ggacatccgg atcaagctgt
     1801 tctatgcccg ggacttccgg gcgatgctgc tcaaatacgg ccgcctcgcg ctcgccgagg
     1861 cgctgtcggg cacgcccggc caggtcgtcc cggagcgcgc ccgcgtggtc gtcgagccgg
     1921 acgccgcgat cgaggtgctc gccgaagtcg aggagcccga agcggccgac gcgccgacgg
     1981 tcgtcatcga agcgccgatc ccgcgcccgc gaccccgcgc gcgcccgggt cgccggcgca
     2041 ggcgttcgag cagcagcaag gtggccgggg ctgagcggga ggcgggtgcg tgacgaccga
     2101 agtcgaccag cgggcggaca tcggcgaggt gctcctcacc gaggaccaga tccaggccaa
     2161 ggtcgccgag ctgggcgcgc agatctcgag cgacctcgca ggccgccagc tgaccctcgt
     2221 cagcgtgctc aagggctcgc tgccgttcat ggccgacctc atgcgcgcca tcagcctgcc
     2281 gctgcggatc gacctgatgg aggtctcgtc gtacggcggc gccacgaccg aatcctcggg
     2341 ccttgtccgg atcctgaagg acctctcgtc gagcatcgag ggcgaggatg tcctgatcgt
     2401 cgaggacatc atcgacacgg ggctgacgct gaactacctc gtccgttatc tccgcggcaa
     2461 gaacccggcg tcgctgcgca tctgcacgct cctcgacaag cccgcgcgcc ggctcgtcga
     2521 gatcgaggtc gactacaccg gcttcaccat cccggaccag ttcgtcgtgg gctacgggct
     2581 cgactacggc gagctgtatc gaaacctgcg cttcgtgggc
//
LOCUS       DATBSW010000042         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1167174, whole genome shotgun sequence.
ACCESSION   DATBSW010000042 DATBSW010000000
VERSION     DATBSW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167174"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VIF63_01145"
     CDS             <1..702
                     /locus_tag="VIF63_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238013.1"
                     /translation="VRALNLKQFILPSPVAIATAWSANLPVLVSAAAYTLTEIVAGLL
                     IGSIAGILVGMVSARFAPIRGTLLPFAIVANSVPIIAFAPIFNNWFGTDTQLSKALIA
                     AVLVFFPVMINTMRGLLNVDPAALELMRSYAAPQWTVFRRLRVPNSLPFIFTALRVAT
                     TLATIGAIVSEYFAAPRGSLGQYIATQSAFLAFERSWAAIIFAAAIGIGLYLAVVALE
                     RLVSPWAQAQPVSAE"
     gene            788..1882
                     /locus_tag="VIF63_01150"
     CDS             788..1882
                     /locus_tag="VIF63_01150"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8238014.1"
                     /translation="MTIRKALALVVVGAFVFIGCNATPAATPAAKTPIKLQLQWFPQA
                     QFAGYFAALDKGFYAEEGLDVTILPGAVDIVPATVVAGGNAEFGISWVPRMLAPRESG
                     ADVQVIGQVFQRSATTQVSFKSKNITSVADLKGKKVGSWGFGNEFELLAGMRKAGIDP
                     DKDVTIVPQQFDMNAFVAGDIDAAQAMTYNEYAQVLEKLNPATNKLYTADDLNVINWE
                     TEGTSMLQDAIFTSEAWLAKTGNDAIAVKFLKASFRGWIFCRDNAAECVDIVLKHDAK
                     LPKGHQTWQLNEVNALIWPSPAGIGIMDQAAFDRTVQISIDSKVLKAAPTGTAFRNDL
                     AQQALAALGSADTKGAAWQKATVTLTQDGK"
     gene            complement(1960..>3194)
                     /gene="chrA"
                     /locus_tag="VIF63_01155"
     CDS             complement(1960..>3194)
                     /gene="chrA"
                     /locus_tag="VIF63_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY8238015.1"
                     /translation="PAPASGMPPADASPARIFGRFLRFGVLAFGGPVAQIAMLRRELV
                     DEERWIYTERFNRLLAVYQVLPGPEAHELAVHLGFLRGGRLGGLLAGLGFMLPGFLLM
                     FGLSWLYFQVDLTQAAIGSALLGAQIAVIALVGRAVVRLSEHILVDRGLVVIAVAGGI
                     ATLAGLSFWIVLPVAGLAHVLVPRAPRGSVHLIVLGAAAAALVAAVASTGAFALPGPV
                     VGPPSPSAFGDPSLAEIFGSGLKAGLLTFGGAYTAIPFLRGDAVGSGWLTDAQFLDGI
                     ALGGILPAPLIIFSTFVGYVAGGPLGAIVMTVGVFLPAFSFTLLLGDRLDAVVDNPAL
                     HRLLEGIAAGVVGLIAVTAFELALAVAARVPSALPALVILGVALAILFTTRARAAIPV
                     VVGGAALAGWLLFGVIGR"
BASE COUNT          574 a         1102 c         1092 g          426 t
ORIGIN      
        1 gtccgcgcct tgaacctgaa gcaattcatc ctgcccagcc cggtggcgat cgcgaccgcc
       61 tggagcgcca acctccccgt cctcgtgtcg gccgcggcgt acaccttgac cgagatcgtc
      121 gcgggtctgc tcatcggctc gattgcgggg atcctcgtcg gcatggtgtc ggcccggttc
      181 gcgccgatcc ggggcacgct tctgccgttc gcgatcgtcg cgaactccgt gccgatcatc
      241 gccttcgccc cgatcttcaa caactggttc gggaccgata cccagctgtc gaaggcgctg
      301 atcgcggcgg tgctggtgtt cttccccgtg atgatcaaca cgatgcgcgg gctgctcaac
      361 gtggatccgg cggcgctcga gctgatgcgc tcgtatgccg cgccgcagtg gacggtgttc
      421 cgccggctcc gggtgccgaa ctcgctgccc ttcatcttca ccgcgctccg ggtggcgacg
      481 acgctcgcca ccatcggcgc gatcgtgagc gagtacttcg ccgcgccgcg cgggagcctc
      541 ggccagtaca tcgccaccca gtccgcgttc ctggcgttcg agcggtcctg ggccgcgatc
      601 atcttcgccg ccgcaatcgg tatcgggttg tacctcgccg tggtcgccct ggagcggctc
      661 gtcagcccat gggcgcaggc gcagcccgtt tccgcagagt gacgccgcgg accaccgctg
      721 gctagactcg cggctggagc gcggcagtct cgcatcggtc tgaggcgccg gccatgagga
      781 ggagagcatg acgatcagaa aggccctggc gctcgtcgtg gtcggggcat tcgtgttcat
      841 cggctgcaac gcgacgccgg ctgccacgcc cgccgcgaag acgccgatca agctgcagct
      901 gcagtggttc ccgcaggccc agttcgcggg ctacttcgcc gcgctggaca aggggttcta
      961 cgccgaggaa gggctcgacg tcaccatcct gccgggcgcg gtcgacatcg tgcctgcgac
     1021 ggtcgttgcc ggcggcaacg ccgagttcgg catctcctgg gtgccgcgca tgctcgcgcc
     1081 gcgcgagagc ggtgccgacg tgcaggtcat cggccaggtc ttccagcgct cggcgaccac
     1141 ccaggtctcc ttcaagtcca agaacatcac cagcgtggct gacctcaagg gcaagaaggt
     1201 cggcagctgg ggcttcggca acgagttcga gctcctcgcc ggcatgcgca aggccggcat
     1261 cgacccggac aaggacgtca ccatcgtgcc gcagcagttc gacatgaacg cgttcgtggc
     1321 cggcgacatc gatgccgcgc aggcgatgac ctacaacgag tacgcccagg tcctcgagaa
     1381 gctcaatccc gcgaccaaca agttgtacac ggccgatgat ctcaacgtca tcaactggga
     1441 gaccgagggc acgtcgatgc tccaggacgc gatcttcacg tccgaggcat ggctcgccaa
     1501 gaccggcaac gacgcgatcg cggtcaagtt cctgaaggcc tcgttccggg gctggatctt
     1561 ctgccgcgac aacgccgccg agtgcgtgga tatcgtcctc aagcacgacg cgaagctgcc
     1621 gaagggccac cagacctggc agctcaacga ggtcaacgcg ctgatctggc cgtccccggc
     1681 gggcatcggg atcatggacc aggcggcatt cgaccgaacc gtccagatct cgatcgattc
     1741 caaggtcctc aaggccgcgc cgaccggcac ggccttccga aacgacctcg cccagcaggc
     1801 gttggcagcc ctcgggtctg ccgacacgaa gggcgctgcc tggcagaagg cgaccgtcac
     1861 gctcacgcag gacggcaagt agccgctccc ttcggcgacg tccgtcgtca aacccgctcc
     1921 gaacgttcga ggccggccca ccgggccggc ctcgacgatt caccggccga tgaccccgaa
     1981 gaggagccac cccgcgaggg cggcgccgcc gacgaccacc gggatcgcgg cccgggcccg
     2041 ggtcgtgaac aggatcgcga gcgcgacgcc gaggatgacg agcgcgggaa gggccgacgg
     2101 gacccgtgcc gcaaccgcca gggcgagctc gaaggcggtg accgcgatga gcccgacgac
     2161 gcccgcggcg atgccctcga gcaggcggtg aagcgccggg ttgtcgacca cggcgtcaag
     2221 gcggtcgccc agcagcaggg tgaacgagaa cgcgggcagg aagacgccga ccgtcatgac
     2281 gatggcaccg agcggaccgc cggcaacgta gccgacgaac gtcgagaaga tgatcaaggg
     2341 cgccggcagg atgccgccga gcgcgatccc gtcgaggaac tgggcatccg tgagccagcc
     2401 gctgccgacc gcatcaccac gaaggaacgg aatcgcggtg taggcgccgc cgaatgtgag
     2461 cagcccggcc ttgaggccgg acccgaagat ctcggcgagg gacgggtcac caaatgccga
     2521 cggcgaaggt ggcccgacca ccggacctgg tagcgcgaag gcgccggtcg acgcgacggc
     2581 cgcgaccagg gcagccgcgg cggcaccgag gacgatcaga tggacggagc ctcgcggcgc
     2641 ccgtggcacg agcacgtggg ccaggccggc gaccgggagg acgatccaga aggacaggcc
     2701 ggcgagggtc gcaatgccgc ccgcaaccgc gatgaccacc agcccacggt caaccaggat
     2761 gtgctcggag agccggacca cggctcgacc gacgagcgcg atgacggcga tctgcgctcc
     2821 gagcagcgcc gaaccgatcg ccgcctgggt caggtcgacc tggaagtaca gccaggacag
     2881 gccgaacatg agcaagaagc ccgggagcat gaagccaaga cctgcgagca ggccgccgag
     2941 ccgtccgccg cggaggaacc cgaggtgcac cgccagctcg tgggcttccg gcccgggcag
     3001 cacctggtac accgcgagga ggcgattgaa ccgctctgtg tagatccagc gctcctcatc
     3061 cacgagctcc cgccggagca tcgcgatctg agcgacaggg ccaccgaagg cgaggacgcc
     3121 gaagcgcagg aaccgaccga agattcgcgc cggtgaagcg tcggcgggcg gcatcccgga
     3181 cgccggagcg ggaa
//
LOCUS       DATBSW010000043         6033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_426708, whole genome shotgun sequence.
ACCESSION   DATBSW010000043 DATBSW010000000
VERSION     DATBSW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426708"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VIF63_01160"
     CDS             complement(<1..1062)
                     /locus_tag="VIF63_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY8238016.1"
                     /translation="MDASTLQLIILPAGIIAVLFAVYLARDVLARDTGTQAMQDVAGT
                     IFEGAVAFIRRQYTTIGILAVVGAVVIGLVITIFETADVADTKTFGLDLGWRTGLAFI
                     VGAACSMASGIIGMYISVKSNVRTAAAARRSLVEAVQVAMRGGAVSGFLVVALSLLGV
                     YGIFTLFGGLSGGTATHDAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGSDLVGKV
                     EKGIPEDDPRNAGVVADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGVFTIAQAA
                     GWPHPEAWIFFPLVVRGFGLLSTIVAIFFVRGKETEDPMNMLNRGYWVTTILSVGALL
                     LTTNVMLQTNGNAPSGVPVW"
     gene            1279..1908
                     /gene="lepB"
                     /locus_tag="VIF63_01165"
     CDS             1279..1908
                     /gene="lepB"
                     /locus_tag="VIF63_01165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8238017.1"
                     /translation="MTQRPALGCLFEVVETLVLTVLIFLGIQTFVAQPYKVQQGSMET
                     TLMPDQYVLVDKLSPRWAPYARGDIVVFDPPETWSSGGGVPFIKRIIGLPNDLVELRD
                     GKVLVNGVELDEPYIFEENGVPQTTDAQAGGASQWLVPVDELLVMGDHRQDSADSRSF
                     GPIEIRHVIGRAWLRYWPFDTFGVLQRPGHPELDGVAVPTLGPASSLAP"
     gene            1905..2618
                     /locus_tag="VIF63_01170"
     CDS             1905..2618
                     /locus_tag="VIF63_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238018.1"
                     /translation="MSIALVAIVLAIGAGAVVAVSTREPAAAAIGLAVALVASALLTD
                     PLPSAAILGVRVVAALLAAALIRWAAHGGPRQFSLLGWPAEALLATAAGVAGLGIAAS
                     LAAAATSSGIPLGEPGGPGIGSTDAGVLNAMALATAAGTSLLALGAAPLVHGDPGLQR
                     AIGRVLVTQAVLLLRIGLAGPATELEEIARAALLVAAAASGSALVRATAVAHVAGRDD
                     EDPEAAVELAASRRPTFSR"
     gene            2629..4089
                     /locus_tag="VIF63_01175"
     CDS             2629..4089
                     /locus_tag="VIF63_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8238019.1"
                     /translation="MTLLLLTGVALGAGLAAILVAESRRRLALAIGLAGSLVVLAIAL
                     SMPAEESVVVGGVALVSTPLVRAMAVAWSASLAVLGLLELAVGGRPTVLGPSLIGLAV
                     GGIAIATHDPASSIAALAAGGVAAVVVPGLNGWIDSPSASSRLTTTSRGTLAILGSGL
                     LGLSVIAWAASAAGPLGGAGIVGPDTDGRLVLGLALIAIVAAVAIRCGLIPLHVWAAR
                     FMEGVSPLAIPAAFAWGGAAFVLVALDWSQVALGPAAAGGVERLIIVTFALVSVILGG
                     LAAMFHDDIEHVLGYSILQDFGIALLAFASLEPEAAAAARNWLVASATVKTAFAGWAA
                     VVRATYGGHRLADLGGWARHSPALGVAFGVILVAAIGLPTMALFDARSSLISGSMPGF
                     GAFVAIVIAAMPAIYLGRIAMAGVAPISVEVESGPSGRPRWSGGRTKGWSEGPLTQAV
                     RAVPAELRANRAPLMAIGVLLLSAMALATSIGGAGG"
     gene            complement(4123..4584)
                     /gene="ndk"
                     /locus_tag="VIF63_01180"
     CDS             complement(4123..4584)
                     /gene="ndk"
                     /locus_tag="VIF63_01180"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY8238020.1"
                     /translation="MTERTLVLVKPDGVQRLLVGRILGRFEERGLKLVGLKLMHVDRA
                     LAERHYAVHREKPFFEGLLAFITSAPLVALALEGPNAIAMVRSMVGATRPHEAAPGSI
                     RGDLAVETAQNLVHASDGAETAAAELALWFEPGELFDYRREVDRWVLAPEE"
     gene            complement(4581..5498)
                     /locus_tag="VIF63_01185"
     CDS             complement(4581..5498)
                     /locus_tag="VIF63_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238021.1"
                     /translation="MTQAGDTAVATPPGQADDIRVARVHLRLGQLTLARAELEDLLRR
                     ELLDTASLAVLAEARWRTGDVAAAAEAAEAHIEGGGSDDVALCIAAEAAAADGRPTDA
                     RALMDRMPESDAATLDAIFAGMPRRAFWPAGPVDRSEIDELRREAEARTVAARRVSAL
                     PRPGAEDDPTTIYRQAPEATDVPERRAIPGPREVLDAVGGADGAPARIAARGGRPGER
                     RMKAQLDPAAELARARTELETRPERAFLRLALVLRHDPTLAPVVLDVLHLRREAAAAL
                     LRGDAQRLLGRHLEAEAAFDAAADSLEVS"
     gene            complement(5495..>6033)
                     /locus_tag="VIF63_01190"
     CDS             complement(5495..>6033)
                     /locus_tag="VIF63_01190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY8238022.1"
                     /translation="PALPRDHPDGWALSVLNAILGDGMSSRLFLSLVDRDALAYDVSS
                     GVVEYADAGALQVAAGVDPARVGRTLDAILRELALLRDEVVGDDELAKAKAYLSGGLE
                     LRMEETRHLASWIGGQEALHDRVLTLDEALEAVARVTSADLTRLARQLFHTELLRLAV
                     VGPARSLRGLDRHLTLPA"
BASE COUNT          764 a         2153 c         2223 g          893 t
ORIGIN      
        1 ccacaccggc acaccggacg gcgcgttgcc attggtctgg agcatcacgt tcgtggtgag
       61 cagcagcgca ccgacggaca ggatcgtggt gacccagtag ccgcggttga gcatgttcat
      121 cggatcctcg gtctccttcc cgcggacgaa gaagatcgcg acgatcgtgg aaagcaggcc
      181 gaagccgcgg acgacgaggg gaaagaagat ccaggcctcg gggtgcggcc agccggcggc
      241 ctgggcgatc gtgaagacgc cgacgccgag gatcatcgcg ccgatgttct cggccgccgt
      301 ggattcgaac aggtccgcac cacggccggc gcagtcgccg acgttgtcgc cgacgaggtc
      361 cgcgacgacg cccgcgttgc gcggatcgtc ttcggggatg cccttctcga ccttgccgac
      421 gaggtcggag ccgacgtcgg cggccttcgt gtagatgccg ccgccgagct gggcgaacag
      481 ggccacgaat gacgccccga agccgaagcc gacgatcagg aacggcgcgt cgtgggtggc
      541 cgtgccaccc gacagcccgc cgaacagggt gaagatgccg tagacgccga gcagggacag
      601 cgcgacgacg aggaagccgg agacggcccc gccgcgcatc gcgacctgga ccgcctcgac
      661 gaggctgcgt cgcgcggcgg ccgccgtgcg gacgttcgac ttgacgctga tgtacatgcc
      721 gatgatcccc gacgccatcg agcaggcggc gccgacgatg aacgcgaggc cggtccgcca
      781 gccgaggtcg agcccgaagg tcttcgtgtc ggcgacatcg gccgtctcga agatggtgat
      841 gaccaggccg atcacgaccg ccccgacgac ggcgaggatg ccgatcgtcg tgtactggcg
      901 gcggatgaag gcgaccgcac cctcgaagat ggtgccggca acgtcctgca tggcctgggt
      961 gccggtgtcg cgggccagca cgtcgcgggc caggtacacg gcaaacagca cggcgatgat
     1021 gccggccggc aggatgatca gttggagcgt gctcgcatcc atcgggcggg gtggtcctcc
     1081 aggctcatgg gttgctgcac gggcgcccgt agggcagggc gtcgctcgtg cgtactgctc
     1141 aggattgggc gggtggcccc tcttcggctt cgtccagcgg gctcagcccg cgcgtcaacg
     1201 agcccctccg ggccgggccg gagtctagca cgggccccgc ccctatactc cccgtcctgt
     1261 gagccagcga cccccaacat gacccaacgc ccggcccttg gctgcctgtt cgaggtcgtc
     1321 gaaaccctcg tcctcacggt cctgatcttc ctcggaatcc agacgttcgt ggcccagccg
     1381 tacaaggtcc agcaggggtc gatggagacc acgctgatgc ccgatcagta cgtcctcgtc
     1441 gacaagctgt ccccgcgctg ggcgccctat gcgcggggcg acatcgtcgt cttcgaccca
     1501 cccgagacct ggtcctcggg cggtggcgtg ccgttcatca agcggatcat cggcctgccc
     1561 aatgatctcg tggagctccg ggacggcaag gtgctcgtga acggggtcga gctcgacgag
     1621 ccgtacatct tcgaggagaa cggcgtcccc cagacgaccg acgcgcaggc gggcggcgcg
     1681 agccagtggc tcgtgcccgt ggacgagctg ctggtcatgg gcgaccaccg ccaggactcg
     1741 gccgactcac gaagcttcgg cccgatcgag atccggcacg tgatcgggcg ggcgtggctg
     1801 cgctactggc cgttcgatac gttcggcgtg ctccagcggc ccgggcaccc ggagctcgac
     1861 ggcgtggccg tcccgaccct cgggcctgcc tcctcgctcg ccccgtgagc atcgccctgg
     1921 tcgcgatcgt gctcgcgatc ggggccggcg ccgtcgtcgc ggtgtcgact cgcgagcccg
     1981 ccgcggcggc aatcgggctt gccgtcgcac tcgtcgcttc ggcgctcctg accgacccac
     2041 tcccgtccgc ggccatcctc ggcgtgcgcg tcgtggccgc gctccttgcc gcagcgttga
     2101 tccgatgggc cgcgcatggt ggcccgcgcc agttctcgct gctgggctgg cccgccgagg
     2161 cattgctggc aactgctgcg ggcgtggccg ggctgggcat cgcggcgagc cttgcggcgg
     2221 cggcgacctc gagcggcatc ccgctgggcg agccgggcgg accgggcatc ggatcgacag
     2281 atgccggcgt cctgaacgcg atggccctgg ccactgcggc cggcacgagc ctgctcgccc
     2341 tgggtgcggc gccattggtc catggcgacc ccggcttgca gcgtgcgatt gggcgggtgc
     2401 tcgtcacgca ggccgtgctg ttgctgcgca tcggcctcgc cggtcctgcg acggagctcg
     2461 aggagatcgc gcgcgcggca ctgctcgtgg ccgcggcggc aagcggttcc gcgctggttc
     2521 gggcaaccgc ggttgcacac gtcgccgggc gggacgacga ggacccggaa gccgcggtcg
     2581 agctcgccgc gtcgcgccgc ccgaccttca gccgatgacg gcgccagcgt gaccctgctc
     2641 ctgcttaccg gcgtggcgct cggcgccggt ctcgcggcga tcctcgtcgc cgagtcgagg
     2701 cgccgtctcg cgctggcgat cgggcttgcg ggcagcctcg tcgtgctcgc gatcgcgctg
     2761 tcgatgccgg ccgaggaatc ggtcgtcgtc ggtggcgtcg cgctggtcag cacgcccctg
     2821 gtccgggcga tggccgtcgc gtggtcggcg agtctcgccg tgctcggcct cctcgagctg
     2881 gcggtcggcg ggcgcccgac ggtgctcggc ccgtcgctca tcggcctcgc ggtcggcggc
     2941 atcgcaatcg ccacgcacga tcctgcgagc tccatcgccg cgctcgccgc aggtggcgtc
     3001 gccgcggtgg tcgtcccggg cctgaacggc tggatcgaca gcccgtcggc gtcgtcgcgg
     3061 ctcacgacca ccagtcgggg cacgctcgcg atcctcggtt cgggcctgct cgggctcagc
     3121 gtgatcgctt gggccgcttc ggcggccggg ccactcggcg gcgccgggat cgtcgggccg
     3181 gacaccgacg gccggctcgt gctcgggctc gccctgatcg cgatcgtcgc ggccgtggcc
     3241 attcgctgcg gcctcatccc gctgcacgtc tgggcggccc ggttcatgga gggggtcagc
     3301 ccgctcgcta tcccggctgc gttcgcgtgg ggtggcgccg cgttcgtcct tgtcgcgctc
     3361 gactggagcc aggtggcgct cggtccggcg gctgcaggcg gcgtcgagcg cctgatcatc
     3421 gtgaccttcg cgctcgtgag cgtgatcctg ggtgggctgg cggcgatgtt ccacgacgac
     3481 atcgagcacg tgctcggcta ttcgatcctg caggacttcg ggatcgcgct gctcgccttc
     3541 gcctcgctcg agccggaggc cgcagctgcg gcacgcaact ggctcgttgc cagtgcaacc
     3601 gtgaagaccg cgttcgcggg ctgggccgcg gtcgtgcgag ccacgtatgg cggccaccgc
     3661 ctcgccgacc tcggcggctg ggcgcgtcac tcgccggcgc tcggcgtcgc gttcggcgtg
     3721 atcctcgtcg ccgcgatcgg cctgccgacg atggccctgt tcgacgcccg ctcgagcctg
     3781 atctcgggct cgatgcctgg gttcggcgcg ttcgtcgcga tcgtcatcgc ggcgatgccc
     3841 gcgatctacc tcggccggat cgccatggcc ggggtggcgc cgatcagcgt ggaggtggag
     3901 tcaggcccgt cgggccggcc gcgctggagc ggcggtcgca ccaagggctg gtcggaggga
     3961 ccgttgacgc aggcggttcg cgccgtgccc gccgagctgc gcgcgaatcg ggcgccgctc
     4021 atggccatcg gcgtgctgct cctgtcggcg atggccctcg cgacgtcgat cggcggtgcg
     4081 gggggctagc ttcgcgggcg gctgggttcg cggttcgccc ggctattcct ccggcgcgag
     4141 cacccagcga tcgacctcgc ggcggtagtc gaacagctca cccggctcga accacagcgc
     4201 gagctcggcg gcggccgtct cggcgccgtc ggacgcgtgg accaggttct gcgccgtctc
     4261 gaccgccagg tcgccccgga tcgagcccgg cgccgcctcg tgcggccggg tcgcgccgac
     4321 catcgaacgg accatcgcaa tcgcgttcgg gccctcgagg gcgagggcga cgagcggcgc
     4381 ggaggtgatg aacgccagca ggccctcgaa gaacggcttc tcgcggtgca ccgcgtagtg
     4441 ccgctccgcg agcgcgcgat cgacgtgcat gagcttcagc ccgaccagct tgagtccgcg
     4501 ctcctcgaag cggccgagga tgcggccgac gagcaggcgc tgcacgccgt cgggcttgac
     4561 caggacgagt gttcgttccg tcacgagacc tccaggctat ctgctgccgc atcgaacgcg
     4621 gcctcggctt ccagatgtcg acccagcagc cgttgggcat cgccgcgcag gagcgcggcc
     4681 gcggcctcac gtcgcaggtg caggacgtcg aggaccacgg gcgcgagggt cgggtcgtgc
     4741 cggagcacga gggcgagccg caggaacgcg cgctcgggcc gcgtctccag ctccgtccgc
     4801 gcccgagcga gctcggcggc aggatcgagc tgcgccttca tgcgtcgctc gcccggacgt
     4861 ccgccccggg ccgcgatccg ggccggtgcg ccgtcggcgc cgccgacggc gtcgagcacc
     4921 tcgcgcgggc cggggatcgc gcggcgttcc ggcacgtcgg tcgcctcggg cgcctgccgg
     4981 tagatcgtcg tcgggtcgtc ctccgcgccc ggcctcggca aggcactcac gcgccgtgcg
     5041 gcgacggtgc gcgcctcggc ctcgcgtcgg agctcatcga tctcgcttcg atcgaccgga
     5101 cccgcgggcc agaacgcccg ccggggcatg cccgcgaaga tggcgtcgag cgtcgccgcg
     5161 tcggactcgg gcatgcggtc catcagggcg cgcgcgtcgg tgggccggcc atcggccgcg
     5221 gccgcttcgg ccgcgatgca cagggcgacg tcgtcggagc ccccgccttc gatgtgcgcc
     5281 tcggcagcct ccgcggcggc ggcgacatcg cccgtccgcc agcgcgcctc ggcaaggacc
     5341 gcgaggctcg cggtgtcgag cagctcgcgc cggagcaggt cttcgagctc ggcgcgggcc
     5401 agggtgagct ggccgaggcg gaggtgaacg cgcgcaaccc ggatgtcgtc cgcctgaccc
     5461 gggggcgtgg ccacggcggt gtcgcctgcc tgcgtcatgc gggcagtgtg aggtgtcggt
     5521 cgagaccgcg aagcgaacgc gccgggccga cgacggccag gcggagcagc tccgtgtgga
     5581 acagctgccg ggcgagccgt gtgagatccg cggatgtgac ccgcgcgacg gcctcgagcg
     5641 cctcgtccag cgtcagcacg cgatcgtgca gcgcttcctg gccgccgatc cacgaggcga
     5701 ggtggcgcgt ctcctccatc cgcagctcga gcccgcccga caggtacgcc ttggccttgg
     5761 ccagctcgtc gtcgccgacg acctcgtcgc gcaggagcgc gagctcgcgc aggatcgcgt
     5821 ccagcgtccg cccgacccgg gccggatcca cgccggcggc cacctggagc gcaccggcat
     5881 ccgcgtactc gacgacgccc gacgacacgt cgtacgcgag cgcatcgcga tcgacgagcg
     5941 agaggaatag gcggctgctc atgccgtcac cgaggatcgc gttgagcacg gacagcgccc
     6001 agccgtcggg atggtcgcgc ggcagcgcgg gca
//
LOCUS       DATBSW010000044         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_743922, whole genome shotgun sequence.
ACCESSION   DATBSW010000044 DATBSW010000000
VERSION     DATBSW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743922"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VIF63_01195"
     CDS             <1..642
                     /locus_tag="VIF63_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8238023.1"
                     /translation="VDLEHGNATEATLLAHLTAIEGTGAAALVRPQSGERLRIGRALD
                     MGAEGVVVPRLDTIEQVVEAVTFLRYPPAGQRGVALLTRGARLGTVNHAGVAALNDDI
                     VGIFQIESPAALEAADAFAATDGVDVLFVGPADLSHSLGIPGQFEHPTYQAALRRVVE
                     ACRAHGKAPGILLYDPASFKPHLDLGYTFVGIGADVSFVNNGIKAALAAARGG"
     gene            721..>1554
                     /locus_tag="VIF63_01200"
     CDS             721..>1554
                     /locus_tag="VIF63_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238024.1"
                     /translation="MRPWAAVVQVPTADGLAWFKACQPQQAFEPRLTAALFARWPDRV
                     VRVLGHDAERAWLLTADAGRSIGSLGNAPELWERVLPRYAELQRGERDHVGDHLAHDV
                     LDLRVTALPARYATMLSRDLPLDPEEVEALRRFEPRFAELCAEVAAESPGDTIQHDDL
                     HVNGVFMLGDELRVMDWGDASIGHPFASLVVTFRFLELINGLAPGDPWFARLRDAYLE
                     PWGQDLVGAFDRAMRVGRFAHAIAWLRHRDPLAGQEKADFDVEYAVVLRRALALIPEP
                     AA"
BASE COUNT          204 a          572 c          540 g          238 t
ORIGIN      
        1 gtcgacctcg agcacggcaa tgcgaccgaa gccacgctgc tcgcgcacct caccgcgatc
       61 gagggcaccg gggcggccgc gctcgtgcgt ccgcaatccg gcgagcggct ccgcatcggc
      121 cgcgcgctcg acatgggcgc cgaaggtgtc gtcgtcccgc gcctcgacac catcgagcag
      181 gtcgtcgagg ccgtcacgtt cctgcgctac ccgccggccg gccagcgcgg cgtcgccctg
      241 ctgacgcgcg gcgcccgact gggcacggtg aatcacgccg gcgtcgccgc gctcaacgac
      301 gacatcgtgg gcatcttcca gatcgagtcg cccgctgcgc tcgaggcggc cgacgcattc
      361 gccgccaccg acggcgtcga tgtcctgttc gtcggtcccg cggacctgtc gcattcgctc
      421 gggatcccgg gccagttcga gcaccccacg taccaggcgg cgctgcgccg tgtcgtcgaa
      481 gcgtgtcgcg cacacggcaa ggcgccgggg atcctcctgt acgaccccgc gtcgttcaag
      541 ccgcatctcg accttggcta cacgttcgtc ggcatcggcg ccgacgtctc gttcgtcaac
      601 aacggcatca aggcggcact tgcggccgca cggggcggct agatcgacct cgacctcgag
      661 gccgccgagg cctggatccg gagcgtcgtc cgcccgaccg gaccgatcga cctggttcgg
      721 gtgcgcccgt gggccgccgt cgtgcaggtg ccgacggccg acggcctggc ctggttcaag
      781 gcgtgccagc cccagcaggc tttcgagcca cgcctgaccg cggcgttgtt cgcgcgctgg
      841 ccggatcggg tcgtccgggt gctcggccac gacgccgagc gcgcatggct cctcacggcg
      901 gacgcgggcc ggtcgatcgg cagcctgggt aacgcaccgg agctctggga gcgggtgctg
      961 ccgcgctacg cggagctgca gcgcggcgag cgggatcatg tcggcgacca cctcgcgcac
     1021 gacgtgctcg acctgcgcgt cacggcgctg cccgcgcgct acgcgacgat gctgtcgcgc
     1081 gacctgccgc tcgatcccga ggaggtcgag gcgcttcgcc gattcgagcc tcggttcgcc
     1141 gagctgtgcg ccgaagtcgc agcggagagc ccgggcgaca cgatccagca tgacgacctg
     1201 cacgtgaacg gcgtgttcat gctcggcgac gagctccggg tcatggactg gggcgatgcg
     1261 tcgatcggcc acccgttcgc gtcgctcgtc gtgacgttcc gcttcctcga gctgatcaac
     1321 gggctcgcgc ccggcgatcc atggttcgcg cggctccgcg acgcctacct ggagccatgg
     1381 ggacaagatc tcgttggcgc gttcgatcgc gccatgcgcg tcggcaggtt tgcccatgcg
     1441 atcgcgtggc tccggcatcg cgatccgctg gccggccagg agaaggccga cttcgacgtc
     1501 gaatacgcgg tcgtgctccg tcgcgcgctc gcgctgatcc ccgaaccggc cgcc
//
LOCUS       DATBSW010000045         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_708582, whole genome shotgun sequence.
ACCESSION   DATBSW010000045 DATBSW010000000
VERSION     DATBSW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708582"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..915
                     /locus_tag="VIF63_01205"
     CDS             103..915
                     /locus_tag="VIF63_01205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase, TIGR04325 family"
                     /protein_id="HEY8238025.1"
                     /translation="MGIIDLGKRVSRNVWRLPVVGQVLERDYARSFAKRPPNQFRGVY
                     PTFAEAEASIPAGNRVGYDHAELAGLYRHRMEKANQSDYAVLFWLKDILDERSFVFDF
                     GGHVGVSYHGWRNYLRYRPGMRWLVHDVPAIVKVGAELARERPSEGLAFTSDVADARG
                     CSIFLAAGALQYVDESLPALLARIGGPLPRHLIVNKMPLYDGETFVTVQSTGRAFHPY
                     RIYNRAAFVAEITALGYRAVDDWVNREQHCEIPFTEGRDIDAYSGYYFVREA"
     gene            1019..2887
                     /locus_tag="VIF63_01210"
     CDS             1019..2887
                     /locus_tag="VIF63_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8238026.1"
                     /translation="MPRLAPGIRLSVIACCLLPAVAAAQPAGNLPIDTFRPAIDSRGY
                     LTINASQPLGHGELSFGLGSLAWGRGLLSFENSGNSYSVDNVVTATLGAAVGLHLGPL
                     ELEFGASLPLSILSGDRGPDDMGAPDNPNDDKDYKLDGQGVGNLGLHLKTRLLRSSRA
                     PYVGVGLIASVYLPTANPVDRFLGDRKAVPQLVGIVDRELAGGRLRLALNAGVRLRPA
                     TSFTDAGDMGAPPTNRTIAAAAELPVGFGAAWALSPGRFDLVGEVFGAVPLAGSSNYQ
                     PLEALGGVKLYLARNSFLSLGAGRGLLPAKGANPDVRAFIGIVFEPNIGDRDGDGIKD
                     DVDRCPDEPEDRDRFEDDDGCPDPDNDHDGILDEDDRCRNIPEDRDGVQDEDGCPEGN
                     PGDRDSDGILDHLDRCPDDAEDFDSFEDGDGCPDLDNDRDKILDLDDLCPLVPEDRDG
                     FDDEDGCPDTDNDRDRILDQSDRCPNEPETYNNVDDRDGCPDKDDPRALIVEQKGRID
                     ILKPINFEYDRDVIKRDSYRILDAIVAALQGSSGIELLEIAGHTDEQGNDAYNLDLSI
                     RRAAAVLRYLTEKGVAPNRLESQGYGETVPVDTRSNPVAWAKNRRVEFLILKHASK"
BASE COUNT          479 a         1066 c         1055 g          429 t
ORIGIN      
        1 cgcgacgaac ctggcggtgg tgggcttcgt ctggatcctc gtccagtacc gcgtgctgtt
       61 cgggatcatc ccccgttagc gaccccgtga tacgaagggg cgatgggcat catcgatctg
      121 ggcaagcgcg tctcacgcaa cgtctggcga ctcccggtgg tgggccaggt gctcgagcga
      181 gactatgcgc gctcgttcgc caagcggccg cccaaccagt tccggggggt ctacccgacg
      241 ttcgccgagg ccgaggcgtc gatcccggcc ggcaaccgcg tcggctacga ccacgccgag
      301 ctggcggggc tataccgcca ccggatggag aaggcgaacc agagcgacta cgccgtgctg
      361 ttctggctca aggacatcct cgacgagcgc tcgttcgtgt tcgacttcgg cggccacgtc
      421 ggcgtctcgt accacggctg gcggaattac ctgcgctacc gcccggggat gcgctggctc
      481 gtccacgacg tgccggcgat cgtgaaggtg ggcgcggagc tggcgcgcga gcgcccgagc
      541 gagggcctcg cgttcaccag cgacgtcgcc gacgcgcgcg gctgctcgat cttcctcgcc
      601 gcgggcgcgc tccagtacgt cgacgagtcg ctgccggcgc tcctcgcgcg gatcggaggc
      661 ccgctgccgc gccacctgat cgtgaacaag atgccgctgt acgacggcga gacgttcgtg
      721 accgtgcagt cgacggggcg cgcgttccac ccctaccgga tctacaaccg cgccgcgttc
      781 gtggcagaga tcacggcgct cggctaccgc gccgtcgacg actgggtcaa ccgcgagcaa
      841 cactgcgaga tcccgttcac cgagggccgc gatatcgacg catacagtgg gtattacttc
      901 gtgcgcgagg cgtagggcgc cgatgtaacc gccgcgcggg tgcggtgcac agtgatccca
      961 gaacctgcga ttgccggccg cgccgagttc cggcgcgacc gatcgcgaga ggaccgctat
     1021 gccacgcctg gctcctggga tccgtctgtc cgtcatcgcc tgctgccttc tgcccgctgt
     1081 cgcggcggcg cagccagcgg ggaacctgcc gatcgatacg ttccggcccg ccatcgattc
     1141 gcgcgggtac ctcacgatca acgcgtcgca gccgctcggg cacggcgagc tgtcgttcgg
     1201 gctgggctcg ctggcgtggg ggcgcgggct gctctcgttc gagaacagcg gcaacagcta
     1261 ctcggtcgac aacgtggtca cggcgacgct cggcgcggcg gtcgggctcc acctgggccc
     1321 gctcgagctc gagttcggcg cgtcgctgcc gctgtcgatc ctgtcgggcg atcgcggccc
     1381 cgacgacatg ggcgcgcccg acaacccgaa cgacgacaag gactacaagc tcgacgggca
     1441 gggcgtgggc aacctcgggc tgcacctgaa gacgcggctc ctgcgatcga gccgggcgcc
     1501 gtacgtcggc gtggggttga tcgcgagcgt gtacctgccg acggcgaacc cggtcgatcg
     1561 cttcctgggc gaccgcaagg cggtcccgca gctcgtgggc atcgtcgatc gggagctggc
     1621 gggcgggcgg ctccggctcg cgctcaacgc cggcgtgcgg ctgcgcccgg cgacgtcgtt
     1681 caccgacgcc ggcgacatgg gcgcgccgcc gacgaaccgg acgatcgcgg cggcggcgga
     1741 gctgccggtc gggttcggcg cggcgtgggc gctgtcgccg ggccggttcg atctggtcgg
     1801 cgaggtgttc ggcgcggtgc cgctcgctgg cagcagcaac taccagccgc tcgaggcgct
     1861 cggcggcgtg aagctctacc tcgcgcgcaa ctcgttcctc tcgctcggcg cgggccgggg
     1921 cctcctgccc gcgaagggcg cgaaccccga cgtgcgcgcg ttcatcggca tcgtgttcga
     1981 gccgaacatc ggcgaccgcg acggcgacgg catcaaggac gacgtcgacc ggtgcccgga
     2041 cgagccggag gaccgggacc gcttcgagga cgacgacggc tgccccgatc ccgacaacga
     2101 ccacgacggg atcctcgacg aggacgaccg ctgccgcaac atccccgagg atcgcgacgg
     2161 ggtccaggac gaggacggct gccccgaggg caacccgggc gatcgcgaca gcgacggcat
     2221 cctcgatcac ctcgaccggt gccccgacga cgccgaggac ttcgattcgt tcgaggacgg
     2281 cgacgggtgc ccggatctcg acaacgatcg cgacaagatc ctcgacctcg acgacctgtg
     2341 cccgctcgtc cccgaggatc gcgacggctt cgacgacgag gacggctgcc cggacacgga
     2401 caacgatcgc gatcggatcc tcgaccagag cgaccgctgc ccgaacgagc cggagaccta
     2461 caacaacgtg gacgaccgcg acggctgtcc ggacaaggac gacccccgcg cgctcatcgt
     2521 cgagcagaaa ggccgcatcg atatcctgaa gccgatcaac ttcgagtacg accgggacgt
     2581 gatcaagcgg gactcgtatc gcatcctcga cgcgatcgtg gcggcgctcc agggcagctc
     2641 cgggatcgag ctgctggaga tcgccggcca caccgacgag cagggcaacg acgcctacaa
     2701 cctcgatctc tcgatccggc gcgccgccgc ggtgctgcgc tacctgaccg agaaaggcgt
     2761 cgctccgaat cggctcgagt cgcagggcta cggcgagacg gtgccggtcg acacgcgcag
     2821 taacccggtg gcgtgggcga agaaccgccg cgtcgagttc ctcatcctga agcacgcgag
     2881 caagtgagcc gcgcagacgc cggccgcgcc cagggagccg cctcgcgagg acgcccggct
     2941 cagactgccc aggtccacat caccggatcg gcgtccatcg gatcggtgac cgtgcgcccg
     3001 atcgcgtcga tcgccgccag atcctcatc
//
LOCUS       DATBSW010000046         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_779335, whole genome shotgun sequence.
ACCESSION   DATBSW010000046 DATBSW010000000
VERSION     DATBSW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779335"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2460)
                     /locus_tag="VIF63_01215"
     CDS             complement(<1..2460)
                     /locus_tag="VIF63_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY8238027.1"
                     /translation="MIGAFSAAAVGVALFLIAAAVVLWVVARRPDRITMLVSLAVLSL
                     AFFILPTRVHERYLFPFAAIAAILAAVSLRWRLAYVLSAAATFANLYVVLVNYYDNPK
                     IDDWIGAGDLFASPWGVAVAAIAQTLVFAWAFFQLREEAVEGVEEGVALSGRERAPGL
                     PGWFGRAAPDADIDIDDVPDDRLDRPLAPAPLGAPPLAPPPRALPGWASSLTRPAPSV
                     AFAGVAAGMAPAAAQRVVADPVVLPAWDLDRDAGTLGPIAWLKARLGDTPARADRSRV
                     LNSERGGRLDRLDVWMLAVLAVSLLTVRMWRLDEPYQMHFDEIYHPRTATEFLQDWRY
                     GISHDIYEWTHPHLAKYAMALGLVAFGEDKVGATGRLGVAVVDAVIETRRDDRLDNTE
                     IEGDRLWVATGSEVRAYDLGTRELAGALSVPDAVALAYDDGSRALYVGTRAGEIRVVD
                     VGLLDATRRGPPVEVESRAFMSIDGPISKLFLTRDGDRLVATLQPGFGTDDPQSSTLV
                     VIDTQAALELGRPVLPGVTQLATGYSDDRIAVATATGVAFIEKVAGGVTQTLDLGGPV
                     GGIVGINDIEDDPIIATVQTADGPKVGLIIAKTGEDARLERTYPLPGRRAGRAYFDLA
                     ARMVHVEGSVPETAGGGFGLSGPPGADTIYVIEPHGSTVGKPDMIYADAPLPFVPAVI
                     VMDDNQKYPSSDRQQLLAFDAGGSVASVEIGRHAYAWRVPGVIAGVLMAMFLYVLARL
                     LFKRRDVAVLLGVIVLLDGMLFAQSRIGMNDSYVGLGIVGAYTIFAALWLKPGNSRRH
                     WIAFALGVPLIGAFLGFALASK"
BASE COUNT          400 a          914 c          926 g          335 t
ORIGIN      
        1 tttcgacgcg agggcaaagc cgaggaacgc gccgatcagg ggcacgccga gggcaaatgc
       61 gatccagtgc cgccggctgt tgccgggctt gagccagagc gcggcgaaga tcgtgtacgc
      121 gcccacgatc ccgagcccga catacgagtc gttcatgccg atccggctct gggcgaacag
      181 catgccgtcg aggagcacga tgaccccgag cagcacggcg acgtcgcggc gcttgaacag
      241 cagccgcgcg agcacgtaca ggaacatcgc catgagcacg ccggcgatca cgcccggcac
      301 gcgccaggcg taggcgtgcc gcccgatctc gaccgaggcc acgctgcccc cggcgtcgaa
      361 cgccaggagc tgctgccgat cggatgacgg gtatttctgg ttgtcgtcca tgacgatcac
      421 cgccggcacg aacggcagtg gcgcatcggc gtagatcatg tccggcttgc cgaccgtgct
      481 gccgtggggc tcgatgacat agatcgtgtc cgcgccgggt gggccggaca ggccgaaccc
      541 accgccggcc gtttcgggca ccgaaccttc gacgtggacc atgcgcgcgg cgaggtcgaa
      601 atacgcgcgg cccgcccgcc tgccgggcag ggggtacgtc cgctccagcc gcgcgtcctc
      661 gcccgtcttc gcgatgatga gcccgacttt cggaccgtcg gcggtctgga cggtcgcgat
      721 gatcgggtcg tcctcgatgt cgttgatccc cacgatccca ccgacggggc cgcccaggtc
      781 gagcgtctgg gtcacgccgc ccgcgacctt ctcgatgaac gccacgccgg ttgccgtcgc
      841 caccgcgatg cgatcgtcgc tgtagccggt cgccagctgg gtgacgccgg gcaggaccgg
      901 ccggccgagc tcgagcgccg cctgcgtgtc gatcacgacg agcgtgctgc tctgtgggtc
      961 gtcggtcccg aagccgggct ggagcgtcgc gaccagccgg tcgccgtcac gcgtcaggaa
     1021 cagcttcgag atcgggccgt cgatcgacat gaaggcgcgc gattcgacct cgaccggcgg
     1081 cccgcgccgg gtcgcgtcga gcagtccgac gtcgacgacg cggatctcgc cggcacgggt
     1141 gccgacgtac agggcacggc tgccgtcgtc gtaggcgagc gcgaccgcat cgggcacgct
     1201 gagggcgccg gcgagctccc gcgtgcccag gtcgtaggcg cggacctcgc tgccggtggc
     1261 gacccacagc cggtcgccct cgatctcggt gttgtccagt cggtcatcgc gccgggtctc
     1321 gatcaccgcg tccacgaccg cgaccccgag gcgccccgtg gcgccgacct tgtcctcgcc
     1381 gaacgcgacg aggccgagcg ccatcgcgta cttggccagg tggggatgcg tccattcgta
     1441 gatgtcgtgc gagatgccgt agcgccagtc ctggaggaac tcggtcgcgg tccgcgggtg
     1501 gtagatctcg tcgaagtgca tctggtacgg ctcatcgagc cgccacatcc ggaccgtgag
     1561 caggctcacg gcgagcacgg cgagcatcca gacgtcgagt cggtcgaggc gcccgccccg
     1621 ctccgagttg aggacgcggc tgcgatccgc ccgagcgggc gtgtcgccga ggcgagcctt
     1681 cagccatgcg atcggcccga gcgtgccggc gtcgcgatcg aggtcccagg ccgggaggac
     1741 gacagggtcc gcgacgacgc gttgggctgc ggccggggcc atgcccgccg cgacgccggc
     1801 gaaggccacg cttggcgccg gccgggtgag cgacgaggcc cagccgggca gcgcgcgcgg
     1861 tggcggcgca agcggcggcg ccccgagcgg cgccggcgca agcggccggt cgagtctgtc
     1921 gtcgggcacg tcgtcgatgt cgatgtccgc atccggggcg gcccgaccga accagcccgg
     1981 caggcccggc gcacgttcgc gtccggacag ggcgacgccc tcctcgacgc cttcgacggc
     2041 ctcttcgcgc agctggaaga acgcccaggc gaacacgagc gtctgcgcga tcgccgccac
     2101 ggcgacaccc cacggcgagg cgaacaggtc gcccgccccg atccagtcgt cgatcttggg
     2161 gttgtcgtag tagttcacca gcacgacgta caggttcgcg aaggtcgccg ccgcggagag
     2221 cacgtaagcg agccgccagc ggagcgagac cgcggcgagg atcgcggcga tggccgcgaa
     2281 cgggaacagg taccgctcat ggacgcgggt cgggaggatg aagaacgcga gcgagaggac
     2341 cgcgagcgag accagcatcg tgatccggtc gggccgcctg gcgaccaccc acaggaccac
     2401 cgcggccgcg atcaggaaca gcgcgacccc gacggcggcc gccgaaaacg ccccgatcat
     2461 caccccggga tcgcacgttt gcgtgctcgc cggccagctc cacacctgcc agtccccgat
     2521 gcggagctcg ccgccaggcg agggcgcgat cgtcgaatcg cacgcccagc tccgg
//
LOCUS       DATBSW010000047         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_391546, whole genome shotgun sequence.
ACCESSION   DATBSW010000047 DATBSW010000000
VERSION     DATBSW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391546"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VIF63_01220"
     CDS             complement(<1..551)
                     /locus_tag="VIF63_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238028.1"
                     /translation="METTWRDRLRYRIDEFFGRGTIALILGLFAVSVLIIVIIATLVT
                     ASGVGGDKPFLQLVWMGLLRTLDPGTMGADEGSPEFLGAMLAVTLGGIFVISALIGIV
                     NTGIQGRLAELRKGKSRVIESGHTVILGWNQQVFTILSELVVANANQRGRSVVILADR
                     DKVEMDDEIRARILDTKGTKIVCR"
     gene            complement(599..1354)
                     /locus_tag="VIF63_01225"
     CDS             complement(599..1354)
                     /locus_tag="VIF63_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8238029.1"
                     /translation="MFNVAAEAYDRFMGRFSSLLSPQMADLAGVHAGQRALDVGCGPG
                     ALTAVLVERLGADAVAAVDPSAPFVEATRMRHPGADIRQATAEAIPFPDGTFDVTMAQ
                     LVVHFMADPVAGLREMARVTRPGGVIAASVWDHAGGKGPLGLFWAVAHELDPDVADES
                     NLAGAREGHLAELFSAAGIGDIQSDVLSADLPLRGFDDWWEPFEGGVGPAGAYVSSLE
                     PGARAALRDRCRQRLPDGPFVMPARAWVAHGTA"
     gene            complement(1354..1839)
                     /locus_tag="VIF63_01230"
     CDS             complement(1354..1839)
                     /locus_tag="VIF63_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8238030.1"
                     /translation="MPVTVPPAGTRGSRFPKFPGPVARFMSRMQTSMFHRQRGGRTQG
                     GVPALLLETIGARSGEVRRAMLGYLEDGPGAWLITASLAGTARNPGWLYNLGRNPIAT
                     IEFGDGRRIRVEATTLAGAEREAAWQRIATEAPEYAKYPTVTDRQIAVLRLVEQPAAP
                     A"
     gene            1914..>2854
                     /locus_tag="VIF63_01235"
     CDS             1914..>2854
                     /locus_tag="VIF63_01235"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEY8238031.1"
                     /translation="MRRLVILTEGQFGAHHAKTAMGVIRYATDKTVAVLDSSIAGRNV
                     SEWMPGHDIPAVATLDEALGLPLPPDTLLIGIAPTGGRLPADWRAKILEAIAAGLEVH
                     SGLHTFLGDDPEFAAAAAARGTRIVDYRRPPERMETAVGRPHSPGKRVILTVGTDCAI
                     GKMSVALELRKAALAQGDKAVFVPTGQTGMMIDGWGVAVDRVISDFVQGTVEWMLEDG
                     ESRGDWLIVEGQGSLDHPAYSSVTLGLIHGATPHAMVMVHKPGLVEHDFDHRSDASYP
                     IAKLPEFIRLHETVAGLVAPSKVVAIALNTMLYPDDD"
BASE COUNT          442 a          991 c         1003 g          418 t
ORIGIN      
        1 cggcacacga tcttggtgcc cttcgtgtcg aggatccggg cccggatctc gtcgtccatc
       61 tcgaccttgt cccggtccgc gaggatgacg acgcttcggc cgcgctggtt tgcgttggcg
      121 acgaccagct cgctcaggat cgtgaagacc tgctggttcc agccgaggat caccgtgtgg
      181 ccggactcga tcacgcgcga cttgcccttg cgcagctccg cgagacggcc ctggatgccg
      241 gtgttgacga tgccgatcag ggcgctgatg acgaagatgc cgccgagagt gaccgcgagc
      301 atcgcgccga ggaattccgg tgagccctcg tcggcgccca tcgtcccggg atcgagcgtg
      361 cgcaggaggc ccatccagac cagctggagg aacggcttgt caccgcccac gcccgatgcg
      421 gtgacgaggg tcgcgatgat cacgatgatc aggacagaga ccgcgaagag accgaggatc
      481 agcgcgatcg tgccgcggcc gaagaactcg tcgatccggt agcgcagccg atcgcgccac
      541 gtcgtttcca tgccgtaggg cctccgcgat aacggtcgcc cgtcagggta gcggcgagtc
      601 aggcggtacc gtgggcgacc caggcacgcg cgggcatcac gaacgggcca tcaggcagcc
      661 gctgccggca gcgatccctg agcgcggctc gtgcgccggg ctcgaggctg ctgacgtacg
      721 cgcccgcggg cccgacgccg ccctcgaacg gctcccacca gtcgtcgaac cctcgcaggg
      781 gaaggtcggc ggacagaacg tcggattgga tgtcgccgat gccggccgcc gagaacagct
      841 ccgcgaggtg cccttcgcgc gcgccggcca ggttggattc gtcggcaacg tccgggtcga
      901 gctcgtgcgc gaccgcccag aacaggccga gcgggccctt gccgcccgcg tgatcccaga
      961 cggacgcagc gatcacgcct ccggggcgcg tcacgcgcgc catctcgcgc aggccggcaa
     1021 ccgggtcggc catgaagtgc acgaccagtt gggccatcgt cacgtcgaac gtgccgtcgg
     1081 ggaacgggat cgcctccgcg gtcgcctggc gaatgtcggc gcccgggtgg cgcatgcgag
     1141 ttgcttccac gaacggtgcc gacggatcga cggccgcgac cgcatcggcg ccgagccgtt
     1201 cgacaaggac ggcggtcaac gcgcccgggc cgcaaccgac gtcgagcgct cgctggccgg
     1261 cgtgcactcc cgccaggtcg gccatctgcg gcgagagcag gctcgagaac cgacccatga
     1321 accggtcata ggcctccgcc gcgacgttga acatcaagca ggcgccgccg gctgttcgac
     1381 gaggcgaagg acggcgatct gccggtcggt aacggttggg tacttcgcgt actcgggtgc
     1441 ctcggtcgca atcctctgcc acgcagcctc gcgctccgcg cccgcgagtg ttgtcgcttc
     1501 gacgcggatg cgtcggccat cgccgaactc gatcgttgcg atcgggttcc ggccgaggtt
     1561 gtacagccac ccgggattgc gagccgtgcc cgccagcgag gccgtgatga gccacgcgcc
     1621 aggtccatcc tccaggtagc cgagcatggc gcgcctgacc tcgccgctcc gcgcaccgat
     1681 ggtctcgagc agcagcgcgg ggacgccgcc ctgcgttcgc ccgccgcgct gccggtggaa
     1741 catcgaggtc tgcatccggc tcatgaagcg ggcgaccgga ccggggaatt tcgggaagcg
     1801 gctgccgcgg gtgcccgcgg gcgggacggt gactggcatc tcaacgggag cgtaaggccg
     1861 tgacgccggg agcgccaagc ccgaacgccc cggccctcgt atcctccaca gccatgcgac
     1921 ggctcgtgat cctgaccgag ggccagttcg gggcccacca tgcgaagacg gcgatgggcg
     1981 tgattcggta cgcgaccgac aagaccgtcg cggtgctgga ttcgtcgatt gccggtcgca
     2041 acgtgtccga gtggatgccc ggccatgaca tcccggccgt ggcgacgctc gacgaggcgc
     2101 ttggcctgcc cttgccgccc gacacactgc tgatcggcat cgccccgacc ggcggacgcc
     2161 tcccggccga ttggcgggcg aagatcctcg aggcgatcgc tgcggggctc gaggtccact
     2221 ccggcctgca caccttcctc ggcgacgacc ccgagttcgc cgccgcggcg gccgcgcgcg
     2281 gtacccggat cgtcgactac cggcgcccgc cggaacggat ggagaccgcg gtcggccggc
     2341 cgcactcgcc gggcaagcgg gtgattctca cggtcgggac cgattgcgcg atcggcaaga
     2401 tgtcggtcgc gctcgagctt cgaaaggccg cgctcgccca gggcgacaag gccgtcttcg
     2461 tgccgacggg ccagaccggg atgatgatcg atggctgggg cgtggcggtc gatcgcgtga
     2521 tcagcgactt cgtccagggc acggtcgagt ggatgctcga ggacggcgag tctcgcggcg
     2581 actggctcat cgtcgagggc cagggttcgc tcgaccaccc ggcgtattcg tcggtgacgc
     2641 tcgggctcat ccacggcgcg acgccgcacg cgatggtgat ggtccacaag ccgggcctcg
     2701 tcgagcacga cttcgaccat cggtccgacg cgtcctaccc gatcgccaag ctgcccgagt
     2761 tcatccgact gcacgagacg gtggcggggc tcgtcgctcc ctcgaaggtc gtcgcgatcg
     2821 cgctgaacac gatgctctac ccggacgacg acga
//
LOCUS       DATBSW010000048         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_814717, whole genome shotgun sequence.
ACCESSION   DATBSW010000048 DATBSW010000000
VERSION     DATBSW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814717"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VIF63_01240"
     CDS             complement(<1..973)
                     /locus_tag="VIF63_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8238032.1"
                     /translation="MRANVAIGPGPWKGTDRSPLTDPRAQRQLRRAIGLFGVIVMPVG
                     LIWIALGLALQDTPLIAIGIVAFAFATYMLLEYRTSGARPASTLATRAAFATNIAIIA
                     TVTAEPVLGIAMAMAATIPAVLVLAHAGRRVVVRFMFLGITTGTYAALAPSYLPWGSH
                     FPHPIDILLPTSTLVVAFAIFNIFLWVTSSQQTATAMELSAAMELSREVAQTLDPKLV
                     ARIIARHIAVAAEVADCTLSTWDRLNDRVVTFAYHPPDRGALIEPTYDLAEFPATLRV
                     LMEGEPCFVDIADPESDPREVGYLKAVGKRSMVILPLVVRGESIGILE"
     gene            complement(1043..>2138)
                     /locus_tag="VIF63_01245"
     CDS             complement(1043..>2138)
                     /locus_tag="VIF63_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8238033.1"
                     /translation="FADGTQIVAAAGETTTAEVTVPAAGISFKCSVPGHADAGMTGEV
                     MVEGATESAKPDDHGGPAPSSDVAADPDAAPYTLYPAEAPALLTGTTHDVDLVIEEKD
                     MTVAEGFVQHVWTFNGTVPGPVIRVKVGDTVRIHLINPASSQVSHSIDFHSSQVAWND
                     EMTSIAPGEEKLYEWKAEYAGVWMYHCGTSPALHHIANGMFGMVIVEPKEGLPPVDAE
                     FSIVQNEWYLGPQGEPASLSKAAAAAPAPDFVVFNGIANQYLDNPLAVATGARVRVFV
                     LDAGPSIDSSFHVVGTIFSSVTKEGIHLLVGNDGNWGSQAMDLSPAQGGIVEFVMPED
                     GLYPMVTHAFNFVGRGALGLFQAGDGVPGT"
BASE COUNT          352 a          685 c          755 g          346 t
ORIGIN      
        1 gctcgaggat cccgatcgac tcgccgcgga cgaccagcgg caggatgacc atgctccgct
       61 tgccgaccgc cttgagatag ccaacctccc gcggatcgct ctccgggtcg gcgatgtcca
      121 cgaagcaggg ctcgccttcc atcaggaccc ggagcgtggc cgggaactcg gcgaggtcgt
      181 aggtcggttc gatcagggcg cctcggtcgg gtgggtggta ggcgaacgtg accacgcgat
      241 cgttcagccg atcccaggtg ctcagggtgc aatcggcgac ctcggccgcg accgcgatgt
      301 ggcgggcgat gatccgggcg accagcttcg ggtcgagtgt ctgcgcgacc tcgcgcgaga
      361 gctccattgc tgcgctgagc tccatcgccg tggcggtctg ctggctgctc gtgacccaca
      421 ggaagatgtt gaagatcgcg aaggcgacga cgagcgtcga tgtcggcagg aggatgtcga
      481 tcgggtgcgg gaaatgcgac ccccacggca ggtacgaggg cgccagcgcc gcgtaggtgc
      541 ccgtcgtgat cccgaggaac atgaatcgca cgaccacccg ccgtccggca tgggcaagca
      601 cgaggacggc tgggatcgtc gccgccatcg ccatcgcgat cccgaggacc ggctcggcgg
      661 tgaccgtcgc gatgatggcg atgttcgttg cgaatgccgc acgagtcgcg agcgtactcg
      721 cgggccgggc tccggacgtt ctgtactcga gcagcatgta cgtcgcgaag gcgaacgcga
      781 cgatgccgat ggcgatcaac ggggtgtcct gcagggcgag cccgagcgcg atccagatca
      841 agccgacggg catgacgatg acgccgaaca gcccgatcgc acgccgcaac tggcgctgcg
      901 cacgagggtc cgtcagcggg cttcgatcgg tgcctttcca ggggccgggg ccaatggcca
      961 cgttggcacg cacatctggc agggtggcga ttggtggaga ttgcggtaat cccccaatcg
     1021 gctgggctgc tgcggtactc gcttacgtgc ccggtacgcc gtcgcccgcc tggaacagcc
     1081 caagcgcgcc ccgtccgacg aagttaaagg cgtgggtgac catggggtac agaccgtcct
     1141 cgggcatcac gaactcaacg attccgccct gcgccggcga caggtccatg gcctgtgagc
     1201 cccagttccc gtcattgccg acgaggaggt ggatgccttc cttcgtgacc gaagagaaga
     1261 tcgtgccgac gacgtggaac gaggagtcga tggaaggccc ggcgtcgagg acgaagaccc
     1321 ggacccgcgc gccggttgcg accgcgagcg gattgtccag gtactggttg gcgatgccgt
     1381 tgaacacgac gaagtcgggc gccggcgcgg ccgcggcggc cttcgacagc gaggcgggct
     1441 cgccctgcgg gccgagatac cactcgttct gcacgatgga gaactcggcg tcgacgggcg
     1501 ggagcccttc cttcggctcg acgatgacca tcccgaacat gccattggcg atgtggtgga
     1561 gcgccggcga ggtgccgcag tgatacatcc acacgccggc gtattcggcc ttccactcgt
     1621 acagcttctc ctcgcccggg gcgatcgacg tcatctcgtc gttccaggca acctggctcg
     1681 aatggaagtc gatcgagtgc gagacctggc tcgaggccgg gttgatcagg tggatccgga
     1741 ccgtgtcgcc gaccttcacc cggatgaccg ggccgggcac ggtgccgttg aacgtccaca
     1801 cgtgctggac gaagccctcg gcaacggtca tgtccttctc ttcgatcacg aggtcgacgt
     1861 catgcgttgt gccggtcagg agcgccggtg cttcggcggg atacagggtg tatggcgcgg
     1921 catccggatc cgcggcgacg tctgacgacg gcgcggggcc gccgtggtcg tcgggctttg
     1981 ccgactcggt cgcgccctcg accatcactt cgccggtcat gcccgcatcc gcgtgaccgg
     2041 ggaccgagca cttgaacgag atgcccgcag cagggacggt gacctcggcc gtcgtggtct
     2101 cgcccgcggc ggcgacgatc tgcgtgccgt ccgcgaag
//
LOCUS       DATBSW010000049         5958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_497428, whole genome shotgun sequence.
ACCESSION   DATBSW010000049 DATBSW010000000
VERSION     DATBSW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497428"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..768)
                     /gene="trmD"
                     /locus_tag="VIF63_01250"
     CDS             complement(94..768)
                     /gene="trmD"
                     /locus_tag="VIF63_01250"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8238034.1"
                     /translation="MTLEIDVVCLFPAMVEGPLSESIPGRTQDRGLAAIRVHDLRQWG
                     IGRHRSVDDAPYGGGAGMVLRPEPVADAIDALRRPDSTVILLDAGGDVFRQGVAASLA
                     ARTHLVLLCPRYEGIDDRIRDLVDLELSIGDYVLTGGELAALIVIDAVTRLLPGAIDD
                     ASTIEESFSAGLLEYPHYTRPAEFRGRGVPDVLVGGNHEAVRRWRHDAAVARTRARRP
                     DLLPPE"
     gene            complement(765..1466)
                     /gene="rimM"
                     /locus_tag="VIF63_01255"
     CDS             complement(765..1466)
                     /gene="rimM"
                     /locus_tag="VIF63_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8238035.1"
                     /translation="MAAPTRLVVAQVRGLHGLNGAVRVEVLTDRPEQRFTTGAVLYLE
                     GDARPLTIIAAQPVEDGPGWRLRFNEAQDRGAAERLRDSYLEIEVDRSADLDAGAAYW
                     HEVIGTTVRDSTGAALGTVADVYRAGENEVYVVRGGPVGEFDMPAVRDVITTFAPERG
                     EIVVDETVLDLGGLPVDGPAPKPSMPKPRRKRWSRHGKGAKPAERPDTTGGAADSTAS
                     PAESDLPGLSETDPR"
     gene            complement(1481..1714)
                     /locus_tag="VIF63_01260"
     CDS             complement(1481..1714)
                     /locus_tag="VIF63_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY8238036.1"
                     /translation="MSAKDLVEYVAKSLVDDPEAVKVTEVEDEEGTVIELHVAEDDMG
                     KVIGRNGSVAKALRTLLKVTAAREGSSVQLEII"
     gene            complement(1711..1974)
                     /gene="rpsP"
                     /locus_tag="VIF63_01265"
     CDS             complement(1711..1974)
                     /gene="rpsP"
                     /locus_tag="VIF63_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8238037.1"
                     /translation="MAVRIRLTRVGATKQPTYRVVVADSRSARDSRSIETLGHYNPRT
                     DPVTFVVDADKAKAWMAKGAQPSDTVARLFRAAGVMPAEKVAK"
     gene            complement(2000..3757)
                     /locus_tag="VIF63_01270"
     CDS             complement(2000..3757)
                     /locus_tag="VIF63_01270"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY8238038.1"
                     /translation="MTDDQTRPMETPVPGGEPAPDAGFDAAPAAPVAPAPAAKGGNGL
                     RWAIALGVAAIAVAIAIGAVALLGAPSSPEALRYIPGDAAMVAELRMDLPGDQMQNLG
                     TLLAHFPGFQDQASLPLKIDEAMSRLFQMSGEGTDYVTDIKPYLSGPTFAALRTLDGA
                     QSGNLSGGLLVATTNGSVACATAFEGQTLTHETYRELDISKGPNGDVACAVDGRFFLV
                     GELASVKLGLDAHAAGTGIDKSAKYAAARTKLGLDRLATFYIEGKALLDAVSAAQPNN
                     PALGLGTAFPEWMAAGVRAEADALVFDAVVAPAPSATLGPDMLSFPPAHPIALAQLAP
                     ADTLFFMELHGAGISFHNAFSQLKSMPELAEPLAALDQVGGLDGLIGWIEDIGVIVTP
                     DGDLASGAVLLVAKDAADATKKATAVTAVLQLGGLGGDVDVDVATVEGVEVTTVHIPD
                     ATALLGQVTGIPGSGVTPTPIPLDFSIAAKDRIIYFGVGQGIMPKILGVKAGASLVDS
                     AAYTHAIARGLADAQQVMYVAAGAGIDLLEKAIPAAELTKWTNDVKPYVDPLEAIIWT
                     VTGDGTQGSVRMALTVAKP"
     gene            complement(3766..5100)
                     /gene="ffh"
                     /locus_tag="VIF63_01275"
     CDS             complement(3766..5100)
                     /gene="ffh"
                     /locus_tag="VIF63_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8238039.1"
                     /translation="MFDSLSDRLRKTLANLTGRGRISEADVDTATREIRLALLEADVN
                     FKVVKDVVGRIREKAVGAEVLQGLGAGQQIVKIVHDELVDLLSAGDRTFHLSGNPAVI
                     SLVGLQGSGKTTTSAKLARHIVKLGRRPLLVAADPYRPAAADQLETLGRALDIPVHRA
                     PAGTKTVDIAKGALETAQRLTRDTIILDTAGRLSLDEALMAEIAAVNKAMHPVETLLV
                     VDAMTGQEAVGVAQAFAAAVPITGLVLTKVDGDARGGAALSIATVTGIGVKFLGTGEK
                     TDALEVFHPDRLAGRILGMGDVLTLVERAQEHVDQAQAAKLEEKLRKAEFTLEDFLDQ
                     LQQVQKMGPIGQLMGMIPGMGDMAKEAEAAVDRGDLRRTEAIIRSMTTRERRDPAILT
                     GSRRRRIATGSGTSLTDVNRLVKQFTEMQRMMKQLSGGRGRGLLGGMSIGRR"
     gene            complement(5205..>5958)
                     /locus_tag="VIF63_01280"
     CDS             complement(5205..>5958)
                     /locus_tag="VIF63_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238040.1"
                     /translation="GQRGTAWYEGLVIHVDSVIAVLDARGGPVELHLRFDNAQEDPGE
                     LDGAIRLHIGETFVDPTRESTVPLVPAKGSAGAVLTYDLQNIASIDEAVIEIGAAPLH
                     IARIPLTPAAGAPALFEPIETVIKGVNTAGSTKITLKRAVLRWDLPDWSQELDAGLAA
                     LTITYDVNYAGDFSGGLAFTGESVALRLPDGTVVEHRADGHSQSVELIGPRKTKKGLF
                     SRFEIPADATGEFALLVRSGSTTKAILFTIDG"
BASE COUNT          825 a         2020 c         2171 g          942 t
ORIGIN      
        1 ggtcgaggcg cgcgtgagcg ccgctggaat tcgattggag atcggctgtc ggccgacggt
       61 cgcggagcat accacgaggc cgcagcgacc cggctactcc ggcgggagca ggtcgggccg
      121 gcgggcgcgg gtgcgggcga ccgcggcatc gtggcgccag cggcgcaccg cctcgtgatt
      181 gccgcccacg aggacgtcgg gcacgccccg gccgcggaac tcggcgggtc gcgtgtagtg
      241 cgggtactcg aggagcccgg cgctgaacga ttcctcgatc gtcgacgcgt cgtcgatcgc
      301 cccgggcagg agccgcgtga cggcatcgat cacgatcagt gccgcgagct cgccgccggt
      361 cagcacgtag tcgccgatgg atagctcgag gtcgaccaga tcgcgaatcc ggtcgtcgat
      421 gccctcgtag cgcgggcaga ggaggaccag gtgcgtacgc gccgccagcg atgccgcgac
      481 cccctggcga aacacgtcgc caccggcatc gaggaggatc acggtcgagt ccggccggcg
      541 gagggcgtcg atcgcgtcgg cgacgggctc gggccgcagg accatccccg ctcccccgcc
      601 gtacggcgcg tcgtcgacgc tgcggtggcg cccgatgccc cactggcgca ggtcgtgcac
      661 gcggatcgcc gccaggccgc gatcctgggt gcggccggga atgctctcgg acagggggcc
      721 ctcgaccatc gccggaaaga ggcagacgac gtcgatctcg agggtcatct ggggtcggtt
      781 tcggaaaggc cggggaggtc ggattcagca gggcttgcgg tcgagtcggc tgcgcctccc
      841 gttgtgtccg gacgttcggc cggctttgcc cctttgccat gcctcgacca tcgcttccgg
      901 cgcggcttcg gcatcgacgg cttcggcgcc gggccatcga ccggcagccc gccgaggtca
      961 aggaccgtct cgtcgacgac gatctcgcca cgctccgggg caaaggtcgt gatcacgtcg
     1021 cggacggcgg gcatgtcgaa ctccccgacc gggccgccgc ggaccacata gacctcgttc
     1081 tcgccggcgc gatagacgtc agcgacggtg ccgagcgcgg cgcccgtcga gtcgcgcacg
     1141 gtcgtgccga tgacctcatg ccaataggcg gcccccgcgt caaggtcagc cgagcggtcg
     1201 acctcgatct cgaggtagct gtcgcgcaat cgctcggcgg cgccgcggtc ctgcgcctcg
     1261 ttgaagcgca ggcgccagcc ggggccgtcc tcgaccggtt gcgccgcgat gatcgtcagc
     1321 gggcgcgcgt caccctcgag gtacaggacg gcgcccgtgg taaatcgctg ttcggggcga
     1381 tccgtgagga cctcgacgcg cacggcgccg ttgaggccgt gcaggcctcg cacctgcgcg
     1441 acgaccaggc gcgtcggcgc cgccacgagg tgctccgcgc tcagatgatc tcgagctgga
     1501 ccgagctgcc ctcgcgggcg gcggtcacct tgagcagggt ccggagcgcc ttggcgacgc
     1561 tgccgttgcg cccgatgacc ttgcccatgt cgtcctcggc cacgtgcagc tcgatgacgg
     1621 tgccctcttc gtcctcgacc tcggtgacct tcacggcctc ggggtcatcg acgagcgact
     1681 tcgccacgta ctcgaccagg tccttggcgc tcactttgcg accttttctg ccggcatgac
     1741 gccggcggcg cggaacaggc gggcgacggt gtccgacggc tgggcgccct tcgccatcca
     1801 ggccttcgcc ttgtcggcgt cgaccacgaa cgtgacgggg tccgtgcgcg ggttgtagtg
     1861 cccgagggtc tcgatggagc ggctgtcgcg ggcgctccgg gaatcggcca cgacgacgcg
     1921 ataggtcggc tgcttcgtcg cgccgacgcg ggtcagtcga atgcgaactg ccatgaatct
     1981 cctccagtac ggtggttggc tagggtttgg cgaccgtcag ggccatgcgc acgctgcctt
     2041 gcgtgccgtc gccggtcacg gtccagatga ttgcctcgag cgggtcgacg tacggcttga
     2101 cgtcgttcgt ccacttggtg agctcggccg cggggattgc cttctccagc aggtcgatcc
     2161 cggcacccgc cgcgacatac atgacctgct gcgcatcggc gaggccgcgc gcaatcgcgt
     2221 gggtgtaggc ggcgctgtcg accaggctcg cgccggcctt gacgccgagg atcttgggca
     2281 tgatcccctg gccgacgccg aagtagatga tccggtcttt cgccgcgatc gagaaatcga
     2341 gcggaatcgg agtcggtgtg acgccgctac ccgggattcc cgtgacctga ccgagcaacg
     2401 cagtcgcgtc ggggatgtga acggtggtga cctccacgcc ctcgacggtc gcgacatcga
     2461 cgtcgacgtc accgccgagg ccgccgagct ggagcaccgc cgtgacggcc gtggccttct
     2521 tggtggcgtc cgccgcgtcc ttcgcaacga gcaggaccgc gcccgaggcc aggtcgccgt
     2581 cgggcgtgac gatgacgccg atgtcctcga tccagccgat gagaccgtcc aacccgccga
     2641 cctggtcgag cgcggcgagc ggctcggcga gctcgggcat ggatttcagc tggctgaagg
     2701 cgttgtggaa cgagatcccg gcgccgtgca gctccatgaa gaagagggtg tcggccgggg
     2761 cgagctgggc cagggcgatg ggatgcgccg gcgggaacga cagcatgtcc gggccgaggg
     2821 ttgcgctcgg cgccggcgcg acgacggcat cgaacaccag ggcatccgct tccgcgcgga
     2881 caccggccgc catccactcg ggaaatgccg tgccaagccc aagggccgga ttgtttggct
     2941 gcgctgcgga gacggcgtcc aggagcgcct tgccctcgat gtagaacgtg gccagtcggt
     3001 cgagaccgag cttggtccgt gcggcggcgt atttcgcgct cttgtcgatg ccggtcccgg
     3061 ccgcgtgcgc gtcgaggccg agcttgaccg aggcgagctc gccgacgagg aagaaccggc
     3121 catcgacggc gcaggcgacg tcgccgttcg ggcccttcga gatgtcgagc tcgcggtagg
     3181 tctcgtgggt cagtgtctgg ccctcgaacg cggtcgcgca ggccaccgac ccgttggtcg
     3241 tcgccacgag gagcccgccg gacaggttgc cggattgcgc accatcgagg gtgcggaggg
     3301 cggcaaacgt cggaccgctg aggtagggct tgatatcggt gacgtaatcg gtgccctcgc
     3361 cgctcatctg gaacaggcgt gacatcgctt cgtcgatctt caggggcagg ctcgcctggt
     3421 cctggaagcc cgggaagtgg gcgagcagcg tgccgaggtt ctgcatctgg tcgcccggca
     3481 ggtccatccg gagctcggca accatggcgg catcgcccgg gatgtagcgc agcgcctccg
     3541 ggctgctcgg tgcgccgagg agcgcgaccg cgccgatggc gatggcaacg gcgatggccg
     3601 ccacgcccag cgcgatggcc cagcgcaagc cgttcccgcc cttcgcggcc ggagccggcg
     3661 ccaccggcgc agcgggggcg gcgtcgaacc cggcatcagg ggcgggctcg ccacccggca
     3721 ccggcgtctc catgggtcgc gtctggtcgt ccgtcatgtt gctccttacc gtcgcccgat
     3781 cgacattcca cccaggaggc cgcggccgcg cccccccgag agctgcttca tcatgcgttg
     3841 catctcggtg aactgcttga cgagccgatt gacgtccgtc agcgacgtcc cggaaccggt
     3901 cgcgatgcgc cgccgccggg agccggtgag gatcgccgga tcgcgccgct cgcgggtcgt
     3961 catcgaccgg atgatcgcct cggtccggcg caggtcgccg cggtcgaccg ccgcctcggc
     4021 ttccttggcc atgtcgccca tgcccgggat catgcccatc agctgcccga tcgggcccat
     4081 cttctggacc tgctggagct ggtcgaggaa gtcctcgagc gtgaactcgg ccttgcgcag
     4141 cttctcctcg agcttggctg cctgcgcctg gtcgacgtgc tcctgcgccc gctcgacgag
     4201 ggtcaggacg tcgcccatgc cgaggatccg gccggccagc ctgtcggggt ggaatacctc
     4261 gagggcgtcg gtcttctcgc ccgtgccgag gaacttcacg ccgatgccgg tcaccgtcgc
     4321 gatcgacagg gcggcgccac cgcgggcgtc gccgtcgacc ttggtcagga cgaggccggt
     4381 gatcggcacc gcggccgcga acgcctgggc gacgcccacc gcctcctgcc ccgtcatcgc
     4441 gtcgacgacg agcagcgtct ccacggggtg catggccttg ttcacggcgg cgatctcggc
     4501 catgagcgcc tcgtcgaggc tcagccggcc cgcagtgtcg aggatgatcg tgtcgcgggt
     4561 gaggcgttgc gcggtctcga gcgcgccctt ggcgatgtcg acggtcttcg tgccggccgg
     4621 cgcccgatgg acggggatgt ccagcgcacg gcccagcgtc tcgagctggt ccgcggccgc
     4681 gggtcggtag gggtccgcgg cgacgaggag tggacgccgg ccgagcttga cgatgtggcg
     4741 cgcgagcttc gccgaggtcg tcgtcttgcc cgagccctgg aggccgacga gcgagatcac
     4801 ggccgggttg cccgacaggt ggaacgtccg atcccccgcc gacagcaggt cgacgagctc
     4861 gtcgtggacg atcttcacga tctgctggcc ggcgccgagt ccctggagaa cctccgcccc
     4921 gacggccttc tcgcggatgc ggccgacgac gtccttcacg accttgaagt tgacgtccgc
     4981 ctcgagcagg gcgagccgga tctcgcgcgt ggcggtgtcg acgtcggcct cgctgatccg
     5041 gccgcggccg gtcaggttgg cgagcgtctt gcgcagtcgg tcgctgaggc tgtcaaacac
     5101 gtgggcgagc ggactccagg cgagacccga tggggacggg agcggcgacg tgccgcggga
     5161 cgagcacgcg ggttctggcg tgcaggcgat cgggagtgta gcccctagcc atcgatggtg
     5221 aacaggatgg ccttggtggt gctgccgctc cggacgagga gggcgaactc gcccgtcgcg
     5281 tcggccggga tctcgaacct cgagaacagc cccttcttcg tcttgcgtgg accgatgagc
     5341 tcgaccgact ggctgtgccc gtcggctcga tgctcgacca ccgtcccgtc gggcaggcgc
     5401 aacgcgacgc tctcgccggt gaaggcgagg ccacccgaga agtcgccggc gtagttgacg
     5461 tcgtaggtga tggtgagggc agccaggccg gcgtcgagct cctgtgacca gtccgggagg
     5521 tcccagcgga gcactgcacg cttgagcgtg atcttcgtcg agccggccgt gttgacgccc
     5581 ttgatcaccg tttcgatcgg ctcgaagagg gccggcgcgc cggcggcggg cgtgagcgga
     5641 atccgcgcga tgtgcaacgg cgcagccccg atctcgatga cggcttcgtc gatcgacgcg
     5701 atgttctgga ggtcatacgt cagcacggcg cccgcgctgc ccttcgcggg cacgagcggg
     5761 accgtggatt cgcgagtcgg gtcgacgaag gtctcgccga tgtgcaggcg gatcgcgccg
     5821 tcgagctcgc ccgggtcctc ctgggcgttg tcgaagcgca ggtggagctc gaccgggccg
     5881 ccgcgcgcgt cgagcacggc gatgacgctg tcgacgtgga tgacgaggcc ctcgtaccaa
     5941 gcggtcccgc gctgtccg
//
LOCUS       DATBSW010000050         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1061707, whole genome shotgun sequence.
ACCESSION   DATBSW010000050 DATBSW010000000
VERSION     DATBSW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061707"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VIF63_01285"
     CDS             <1..407
                     /locus_tag="VIF63_01285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HEY8238041.1"
                     /translation="ARLITEDFLSGDYGRIDLVYSRFISTLSQRPVMVQLLPIQPTPD
                     TEGIPGNQFIFEPSPAAVLEQLLPRYVATRLFHAVLEAKASEESSRMVAMKNATENAG
                     ELIDDLTLAYNKVRQSNITREMIEIATGARAR"
     gene            435..1847
                     /gene="atpD"
                     /locus_tag="VIF63_01290"
     CDS             435..1847
                     /gene="atpD"
                     /locus_tag="VIF63_01290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY8238042.1"
                     /translation="MASAPPITEGRVIQITGPVVDIEFPAGQLPQILNAVEIKRDGQE
                     DLVCEVAQHLGNNWVRTVAMTTTDGLARGTRVVDTGAPITVPVGEATLGRVFDVLGKP
                     IDMKGPVNAEVMLPIHRKPPSFDDQVTEVEVFETGLKIIDLICPFKKGGKIAIFGGAG
                     VGKTVVIQELIRNVAQEHAGVSVFAGVGERSREGNDLIHEMTESGVIAKTAFVFGQMN
                     EPPGARQRVGLTGLTMAEYFRDEEGRDVLLFIDNVFRFTQAGAEVSALLGRMPSAVGY
                     QPNLATEMAALQERITSTKKGSITSLQAVFVPADDYTDPAPATTFAHLDSTIRLERNI
                     AELGIYPAVDPLTSTSRVLDPLVVGQEHYDVARETQRVLQRYRDLQDIIAILGIDELS
                     EEDKATVARARRLQRFASQPFFVAEVFTGRPGIYVPIKDTVTSFKDILEGKGDDLPEQ
                     AFFLAGTLDDVRANAAKLSA"
     gene            1850..2278
                     /locus_tag="VIF63_01295"
     CDS             1850..2278
                     /locus_tag="VIF63_01295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY8238043.1"
                     /translation="MPILLEIVTPERLAYSDNVDSVVLPGSEGEMGVLPHHAPLLATL
                     GVGELRIRKGGEEEFFAIAGGFVQVRPDKVVVMAETADMASEIDLEKAQEARREAEKA
                     LESGFDEGADLAVARAQLQTALLRIRLAERRHHEGARRRL"
     gene            2347..3690
                     /gene="murA"
                     /locus_tag="VIF63_01300"
     CDS             2347..3690
                     /gene="murA"
                     /locus_tag="VIF63_01300"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8238044.1"
                     /translation="MADARPFHWEYEPPAVVRERFLVTGGAALEGTVRVSGAKNAALK
                     MLAAATLTGERCRFSNVPEIEDVRAMAETLADLGVVVDHPEPNVYEVSAGDVDWLFVP
                     LEAAAKMRASFMLLGPLLARFGEVVISNPGGDRIGRRPVDLHVDAMRAMGATIDYRYG
                     YYYAKAPGRLRAAEIRFPMVSVMGTENAMLAATLADGHTVIRPAAQEPEVDDLIAFLG
                     KMGADVQRTYPDTIEIEGKRRLRGAEHSVMPDRIEAGTFAIAAGVTGGHVILEGADCD
                     TMDALVEVMGKAGLAVSCSPGRMEIDARGLGKGGMKAVDVETGAYPALATDIQPPTTV
                     LLTQAKGDSKVHESIFEDRLEWTGELKKMGARIDVKSPQRAVVHGPSKLHAATVEISD
                     LRAGASLILAALAADGTSSIGGAHHVRRGYENIERKFLDLGARIERVQEGGSTPA"
     gene            3687..>4154
                     /locus_tag="VIF63_01305"
     CDS             3687..>4154
                     /locus_tag="VIF63_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8238045.1"
                     /translation="MNIGIDLGTANVLVHVEGKGIVIQEPSVVAVDDDGRIRAVGEDA
                     REMIGRTPGNIHAIRPMKDGVIADYVITEAMLRFFINKAMSGRMRMRLSGPAVMISVP
                     AGVTSVEKRAVKDAALKAGAREAHLIEEPLAAAIGANVPISGPSGNMVIDIGGG"
BASE COUNT          694 a         1503 c         1377 g          580 t
ORIGIN      
        1 tggcccgcct gatcaccgag gacttcctgt ccggcgacta cggccggatc gaccttgtct
       61 acagccggtt catctcgacc ctctcacagc gcccggtgat ggtccagctg ctccccatcc
      121 agccgacgcc cgacaccgag ggcatccccg gcaaccagtt catcttcgag cccagccccg
      181 cggccgtcct cgagcagctc ctgccccgct acgtcgccac ccgcctgttc cacgcggtcc
      241 tcgaggcgaa ggcgagcgag gaatcgagcc ggatggtcgc gatgaagaac gccaccgaga
      301 acgcgggcga gctcatcgac gacctgaccc ttgcctacaa caaggtccgc cagtcgaaca
      361 tcacccgcga aatgatcgag atcgccaccg gcgcccgagc gcgctgaccc gagccttcag
      421 gaggacgaga caccatggcc agcgcaccgc ccatcaccga gggacgggtc atccagatca
      481 cgggcccggt cgtcgacatc gagttcccgg caggccagct gccccagatc ctcaacgccg
      541 tcgagatcaa gcgtgacggc caggaggacc tcgtctgcga ggtcgcccag cacctgggca
      601 acaactgggt gcggaccgtc gcgatgacga cgaccgacgg gctggcccgc ggcacgcgcg
      661 tcgtcgacac cggcgcgccg atcacggtgc ccgtcggcga ggcgaccctg ggccgcgtgt
      721 tcgacgtgct cggcaagccg atcgacatga agggcccggt caacgccgag gtcatgctgc
      781 cgatccaccg caagcccccg tccttcgacg accaggtgac cgaggtcgag gtcttcgaga
      841 ccggcctcaa gatcatcgac ctgatctgcc cgttcaagaa gggcggcaag atcgcgatct
      901 tcggcggcgc cggcgtcggc aagacggtcg tgatccagga gctcatccgc aacgtcgccc
      961 aggagcacgc gggcgtgtcg gtgttcgccg gcgtcggcga gcgcagccgc gagggcaacg
     1021 acctcatcca cgagatgacc gagtcgggcg tcatcgccaa gaccgcgttc gtgttcggcc
     1081 agatgaacga gccgccgggc gcgcgccagc gggtcggcct gaccggcttg acgatggccg
     1141 agtacttccg cgacgaggag ggccgcgacg tcctcctgtt catcgacaac gtcttccggt
     1201 tcacccaggc cggcgccgag gtctcggcgc tcctcgggcg catgccgtcg gccgtcggct
     1261 accagcccaa cctggccacc gagatggccg cgctccagga gcgcatcacc tcgacgaaga
     1321 agggctcgat cacctcgctc caggcggtgt tcgtccccgc cgacgactac accgacccgg
     1381 cgccggccac gacgttcgcc cacctcgact cgacgattcg cctcgagcgc aacatcgccg
     1441 agctcggcat ctaccccgcg gtcgatccgc tgacctcgac gtcgcgcgtc ctcgacccgc
     1501 tcgtcgtggg ccaggagcac tacgacgttg cacgtgaaac acagcgcgtg ctccagcgct
     1561 accgtgacct ccaggacatc atcgcgatcc tgggcatcga cgagctgtcc gaggaggaca
     1621 aggccacggt tgcccgcgcg cggcgcctcc agcgcttcgc ctcgcagccg ttcttcgtcg
     1681 ccgaggtgtt cacgggccgg cccggcatct acgtgccgat caaggacacc gtgacctcgt
     1741 tcaaggacat cctcgagggc aagggcgacg acctgccgga gcaggctttc ttcctcgccg
     1801 gcacgctcga cgacgtccgc gccaacgcgg cgaagctgag cgcgtagcga tgccgatcct
     1861 cctcgagatc gtcacgcccg agcggctcgc gtacagcgac aacgtcgatt cggtcgtgct
     1921 gcccggctcc gagggcgaga tgggcgtcct gccccatcac gccccgctgc tggccacgct
     1981 cggcgtcggc gagctgcgca tccgcaaggg tggcgaggag gagttcttcg cgatcgccgg
     2041 tgggttcgtc caggtccggc ccgacaaggt cgtggtcatg gccgagacgg ccgacatggc
     2101 gtccgagatc gacctcgaga aggcccagga ggcccgccgc gaggccgaga aggcgctcga
     2161 gtccggcttc gacgagggcg ccgatctcgc ggttgcccgc gcccagctcc agacggcgct
     2221 gctgcggatc cgcctcgccg agcgccgtca tcacgagggc gcgcgccggc ggctctagct
     2281 cgggtcgccg cacgagcgca gaattcggga tcacacatga ggcacttcga ccgttgactc
     2341 cactccatgg ctgacgcacg accgttccac tgggaatacg agccgcccgc ggtcgtccgc
     2401 gagcgcttcc tcgtgaccgg tggcgcggcc ctcgagggca ccgtgcgcgt gagtggcgcc
     2461 aagaacgccg cgctcaagat gcttgccgcg gcgaccctga ccggcgagcg ctgccggttc
     2521 agcaacgtcc ccgagatcga ggacgtgcgt gccatggccg agaccctcgc cgacctcggc
     2581 gtcgtcgtcg accaccccga gccgaacgtc tacgaggtct ccgcgggcga cgtcgactgg
     2641 ctgttcgtgc ccctcgaggc ggcggccaag atgcgcgcca gcttcatgct cctcgggccg
     2701 ctcctggctc gcttcggcga ggtcgtcatc agcaaccccg gcggcgatcg gatcggccgg
     2761 cggccggtcg acctccacgt cgacgcgatg cgggcgatgg gcgccacgat cgactaccgc
     2821 tacggctact actacgccaa ggcgccgggt cgtctccgcg ccgcggagat ccgcttcccc
     2881 atggtgtcgg tcatgggcac ggagaacgcg atgctcgccg cgaccctggc ggacggccac
     2941 acggtgatcc ggcccgcggc gcaggagccc gaggtcgacg acctcatcgc gttcctgggc
     3001 aagatgggcg ccgacgtcca gcgaacctac ccggacacca tcgagatcga gggcaagcgc
     3061 cgcctgcgcg gcgccgaaca ctcggtgatg ccggatcgca tcgaagccgg cacattcgcc
     3121 atcgccgccg gcgtgaccgg tggccacgtc atcctcgagg gcgccgactg cgacacgatg
     3181 gacgccctcg tcgaggtcat gggcaaagca ggcctcgcgg tgagctgcag cccgggccgc
     3241 atggagatcg atgcccgcgg cctcggcaag ggcggcatga aggcggtcga tgtcgagacc
     3301 ggcgcgtatc ccgcactcgc gacggacatc cagcccccga cgacagtgct gctgacccag
     3361 gcgaagggcg attccaaggt ccacgagtcg atcttcgagg accgcctcga gtggaccggc
     3421 gagctcaaga agatgggcgc caggatcgac gtgaagagcc cgcagcgggc cgtcgtccac
     3481 ggcccctcga agctccacgc cgcgaccgtc gagatctccg acctgcgcgc cggcgcctcg
     3541 ttgatcctcg ccgcgctcgc cgcggacggc acctcgtcga tcggcggtgc gcaccacgtc
     3601 cggcgcgggt atgagaacat cgaacggaaa ttcctggacc tcggcgccag gatcgagcgc
     3661 gtccaggaag ggggcagcac gcccgcatga acatcggcat cgatctcggc acggccaacg
     3721 tcctggtcca cgtcgaaggc aagggcatcg tgatccagga gccgtctgtc gtggcggtcg
     3781 acgacgacgg gcggatccgc gccgtgggcg aggacgcgcg cgagatgatc gggcgtaccc
     3841 cgggcaacat ccacgccatc cggccgatga aggacggcgt catcgccgac tacgtcatca
     3901 ccgaggcgat gctccgcttc ttcatcaaca aggccatgag tgggcgcatg cgcatgcggc
     3961 tgagcggccc ggccgtgatg atctcggtgc ccgcgggcgt gacctcggtc gagaagcgcg
     4021 ccgtcaagga cgcagcgctc aaggccgggg cgcgcgaggc gcacctgatc gaggagcccc
     4081 tcgctgccgc catcggcgcc aacgtgccga tctcgggacc gtccgggaac atggtcatcg
     4141 acatcggcgg cggc
//
LOCUS       DATBSW010000051         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1238260, whole genome shotgun sequence.
ACCESSION   DATBSW010000051 DATBSW010000000
VERSION     DATBSW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238260"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..801
                     /gene="rplE"
                     /locus_tag="VIF63_01310"
     CDS             271..801
                     /gene="rplE"
                     /locus_tag="VIF63_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY8238046.1"
                     /translation="MRIRYKTEVVPALVKQFEYANPNQVPKLEKIVVNIGLGEALSNA
                     KAVDAASSDLTMITGQKPIVTKARRSIAQFKIRTGNPIGLKVTLRGPRMWDFLERLIL
                     LTLPRFRDFRGTPSRSFDGRGNYSLGMREQIAFPEIDYDKVDRLRGLEVSIVTTAKSD
                     EESKRLLELLGMPFAS"
     gene            819..1004
                     /locus_tag="VIF63_01315"
     CDS             819..1004
                     /locus_tag="VIF63_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY8238047.1"
                     /translation="MAKKSMIAKAKRTPKFGVRGYNRCVVCGRPRAFMRRFNLCRICF
                     RERALVGELPGVTKSSW"
     gene            1010..1408
                     /gene="rpsH"
                     /locus_tag="VIF63_01320"
     CDS             1010..1408
                     /gene="rpsH"
                     /locus_tag="VIF63_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY8238048.1"
                     /translation="MNISDPIADMLTRVRNASRARHTEVLVPASRTKREIAKILQSEG
                     FIAGFEEEQAGPGHVLRLTLRYVDGKVPVVSGLKRVSKPGLRVYAAKTDIPRVLGGLG
                     IVIVSTSQGIMTGAQARKAQLGGEVLAYVW"
     gene            1414..1965
                     /gene="rplF"
                     /locus_tag="VIF63_01325"
     CDS             1414..1965
                     /gene="rplF"
                     /locus_tag="VIF63_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY8238049.1"
                     /translation="MSRIGRLPIAVPAGVDVSIDGRNVTVKGPKGSLSRELHAEIGVA
                     RDGENIVVTRPSEAKTHKQLHGLTRTLVNNMVVGVTTGYRKPLEITGVGYRAALVGRK
                     LQLNLGYSHPIEIDPPQGISFEVENPTKLAVIGIDKELVGEIAARVRATRKPEPYKGK
                     GVRYAGEVIRRKAGKAGKIGGKK"
     gene            1997..2359
                     /gene="rplR"
                     /locus_tag="VIF63_01330"
     CDS             1997..2359
                     /gene="rplR"
                     /locus_tag="VIF63_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY8238050.1"
                     /translation="MTASSSRGAARKKRHVRLRLRVAGDAARPRLAVFRSLNHIYAQV
                     IDDSSGRTLAAASSLEPDMKSAKGTKSAEASAVGRIVAERARAAGVGQVVFDRAGFRY
                     HGRVKSLADAAREAGLDF"
     gene            2370..2903
                     /gene="rpsE"
                     /locus_tag="VIF63_01335"
     CDS             2370..2903
                     /gene="rpsE"
                     /locus_tag="VIF63_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY8238051.1"
                     /translation="MPRIDANKLTLEERVVQINRVAKVVKGGRRFSFSAVIVVGDGAG
                     HVGVGLGKAGEVPEAIRKGVEDAKKNIIKIPMVGTTIPHEVRTVFAASNVLLKPASQG
                     TGVIAGGSVRAVVEAAGIRDILSKVYGSTNPVNVTRATLEGLRSLKSAAELSARRGVP
                     VRLYIPGQPLAEAADAR"
BASE COUNT          523 a         1089 c         1020 g          399 t
ORIGIN      
        1 gctgccgagg cgcccgcgac tgccgccgac gaggcgccgg ttgctgtcga ggaggcgacc
       61 gctgcttccg ccgagccgac cgcagccgtc gacgagcccg ttgcggcctc ggacgagccg
      121 acagccgacg cccagaaggc gtcgaagtcg aaggcgcctg cgaaggcccc gaaggccgcg
      181 gccgaggagc gcaaggccgc cgcggccgcc gcgcccgaag cccccgctcc ggcgcgggcc
      241 gcgaagccgg tcctggaagg gccgcgcccg ctgcgcatcc ggtacaagac cgaggtcgtg
      301 cccgcgctcg tcaagcagtt cgagtacgcc aacccgaacc aggtgccgaa gctcgagaag
      361 atcgtggtca acatcggcct tggcgaggca ctgtcgaatg cgaaggccgt cgacgccgcc
      421 tcgagcgacc tgacgatgat caccggccag aagccgatcg tgaccaaggc ccggcgctcg
      481 atcgcccagt tcaagatccg caccggcaac ccgatcgggc tcaaggtcac gctccggggc
      541 ccgcgcatgt gggacttcct ggagcgcctg atcctgctca ccctgccccg gttccgtgat
      601 ttccggggca cgcccagccg ctcgttcgac ggccgcggca actactcgct ggggatgcgc
      661 gagcagatcg cgttccccga gatcgactac gacaaggtcg accgtctccg cgggctggag
      721 gtcagcatcg tgacgacggc gaagtccgac gaggagtcga agcgtctgct cgagctcctc
      781 ggcatgccct tcgcctcgta ggcgcgggag gagaccaaat ggccaagaaa tcgatgatcg
      841 caaaggcaaa gcggacgccg aagttcggcg tgcgcggcta caaccgctgc gtcgtgtgcg
      901 gccggccgcg cgcgttcatg cgtcgcttca acctctgccg catctgcttc cgggagcggg
      961 ccctcgtggg tgagctgccg ggcgtcacca agtcgagctg gtaggaccca tgaacatctc
     1021 tgatccgatc gccgacatgc tcacccgcgt ccgcaacgcg tcgcgcgccc gccacaccga
     1081 ggtgctggtc ccggcgtcgc gcacgaagcg cgagatcgcc aagatcctcc agagcgaagg
     1141 cttcatcgcc gggttcgagg aggagcaggc cggtccgggc cacgtgctgc ggctgacgct
     1201 gcgctacgtc gacggcaagg tgccggtcgt gtcgggcctc aagcgcgtga gcaagccggg
     1261 cctccgggtg tatgcggcca agaccgacat cccgcgcgtc ctgggcggac tcggcatcgt
     1321 catcgtcagc accagccagg ggatcatgac cggcgcgcag gcccgcaagg cccagctcgg
     1381 cggcgaagtc ctcgcgtacg tctggtagcc gagatgtcac gaatcggacg cctgcccatc
     1441 gccgttcccg ccggggtcga cgtgtcgatc gacggccgga acgtcacggt caaggggccc
     1501 aagggcagcc tgtcccgtga gctccacgcc gagatcggcg tcgcccgcga cggcgagaac
     1561 atcgtcgtca cgcgcccgag cgaggcgaag acgcacaagc agctccacgg cctgacccgc
     1621 accctcgtga acaacatggt ggtcggcgtg accaccggct accgcaagcc gctcgagatc
     1681 accggagtcg ggtatcgtgc agcgctcgtc gggcgcaagc tgcagctgaa cctcggctac
     1741 agccacccca tcgagatcga cccgccgcag ggcatcagct tcgaggtcga gaacccgacg
     1801 aagctggccg tgatcggcat cgacaaggag ctcgtcggcg agatcgccgc acgggttcgc
     1861 gccacgcgca agccggagcc ctacaagggc aagggcgtgc gctacgccgg cgaagtgatc
     1921 cgccgcaagg ccggcaaggc cggcaagatc ggcggcaaga agtaacccgc cgagcgaaag
     1981 accgaggaac catcgaatga ccgcatcgtc cagccgcggg gccgcccgca agaagcgcca
     2041 cgtacgcctc cgcctccggg tggcgggtga cgcggcacgc ccgcgcctcg cggtcttccg
     2101 cagcctgaac cacatctacg cccaggtcat cgacgactcg tccggccgga cgctcgccgc
     2161 ggcctcgtcg ctcgagcccg acatgaagtc ggccaagggc acgaagtccg ccgaagcctc
     2221 cgccgtcggg cgcatcgtcg ccgagcgggc tcgtgccgcc ggtgtcggcc aggtcgtctt
     2281 cgaccgtgcc gggttccggt accacggtcg ggtcaagtca ctcgccgatg cggctcgcga
     2341 agccggcctg gatttctgag gagcctgcat tgccacgaat tgacgccaac aagctgacgc
     2401 tcgaagagcg cgtcgtccag atcaaccgtg tcgcgaaggt cgtcaagggc ggccggcgct
     2461 tcagcttcag cgcggtcatc gtcgtcggcg acggcgcggg ccacgtcggg gttgggctcg
     2521 gcaaggccgg cgaagtgccc gaggcgatcc gcaagggcgt cgaggacgcg aagaagaaca
     2581 tcatcaagat cccgatggtc ggaacgacga tcccgcacga ggtccggacc gtcttcgcgg
     2641 cgagcaacgt gctcctcaag cccgccagcc agggcaccgg cgtcatcgcc ggcggctccg
     2701 tgcgcgcggt cgtcgaggct gccggcatcc gcgacatcct ctcgaaggtg tacggctcga
     2761 ccaacccggt caacgtgacc cgcgccaccc tcgagggcct gcgcagcctc aagtccgccg
     2821 ccgagctcag cgcccgccgc ggcgtgcccg ttcgcctgta catccccggc cagccgctcg
     2881 ccgaggccgc cgatgcccgc taagtccgct ggggcgaccc tcaaggtcac ccagatcaag
     2941 agctcgatca gccatatcgc ccgcaaccgg gccacgctgc gggcgctcgg cctgcacggc
     3001 atcggctcgt cggtcgaagt gcccgccaac g
//
LOCUS       DATBSW010000052         4485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_321543, whole genome shotgun sequence.
ACCESSION   DATBSW010000052 DATBSW010000000
VERSION     DATBSW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321543"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VIF63_01340"
     CDS             complement(<1..427)
                     /locus_tag="VIF63_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238052.1"
                     /translation="MPIALAAAPATGMLAATRKLLRSPNGFAGLAILAFFTVLAIAPD
                     FFVGPLQTAVTAPGTRLEPPSGTFPFGTDELGRSILNLTVHATRISMVIGLLATVITV
                     AFGAVIGILSGYVGGRFDAFMMRIADFFLVLPTFVLCIIL"
     gene            complement(456..1433)
                     /locus_tag="VIF63_01345"
     CDS             complement(456..1433)
                     /locus_tag="VIF63_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238053.1"
                     /translation="MGVRYIARRLVTAAVTIFFIICLNFVLFRMMPGSPERILLGRIP
                     GISQQVLETTKARWGLDKPLFPDQFVAYVSSTLRGDLGFSFVSRGRPVTEVLGSRLGP
                     TLLLFGLGEILAIVLGLALGAYSGWRRGGIVDYIGNGISLILYSTPYFLLGMGLLLLF
                     ATGLGWFPTYGMTDVGRVYDGPLDELSDIAWHLFLPLATVSLGLVGQYAILMRSSVVE
                     TLSEDYITTAKAKGIGDGRVLRAHAIPNALLPTVTLVAINLGYVVAGAITVEVVFNWP
                     GLGTLTVDALSGRDYPVLQGVFLLLSVTVVLANLIADVVYGYLDPRVRT"
     gene            complement(1502..3400)
                     /locus_tag="VIF63_01350"
     CDS             complement(1502..3400)
                     /locus_tag="VIF63_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8238054.1"
                     /translation="MKHARAFLASLMLLAPTLLSAGGAQAGTEELILRIGTDQKLESI
                     DPFQAITYADYEVFQIQYELLVSFGPNLEAVPGFADEWSSSADKKTHTFHIRDGMKWS
                     DGEPATCADVQYTFKLFLDAAETETGYLGSGYLDPYLTNTGLTSAKCDANNNLIAITE
                     FPTTLITQTYIPIVPKHVWSKYSLEEIGNLEADGYFGNKDLPVVGSGPYVVSEFEPGK
                     FVRFDRNPNYWGTNKGVPDAIIMEQFSTNDAMVQALKNKQLDYVRGTGANQFDALATE
                     PNVARSEGYANGYTYLSFNTGGTKEGYHGSTSALEDQKFRDALGYAIDRQELVDKVLN
                     GHGVAGSTHVPPYHVKWHVEPTTPRTFDIPKANSLLDAAGYAKGADGIRVDKEGKPIN
                     LRLTWPDSEDHSADAQFIQGWFAQIGIGVDASVTEEGALIKALYGPDADGPANWDFYM
                     WGWGGDPDPTSLLGTFTTDNIPSGYNDCFYSDARYDELFNEQLHALDETVRKTAIAEM
                     QNLFYDAACYHVLYYDSELHAYRTDKFGGWVNQPPDTGTPFFGFGYSGYMALTDASAV
                     PTPGPTVAATPVPPGVTAGPAATPTPTPTPTASDSTPLLIGGVIVILVIVGGLLFMRR
                     RGAAKEEE"
     gene            complement(3563..>4485)
                     /locus_tag="VIF63_01355"
     CDS             complement(3563..>4485)
                     /locus_tag="VIF63_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8238055.1"
                     /translation="TMTGGLDLCPPNAAESIDDYATAMTEVGVPFEWLDATDVARRWP
                     QWRLDPNTRAIFQADAGIADPEPGNAAHQRLARAAGATLLERQRVTAIHDQGNEIEVV
                     TEGGETFSTGAVVVATDAWTNDLLAPLGHELPLSITREQVTWFEPKDAAAFEPARFPI
                     WIWLDQPSFYGFPTYRSAGPKVGEDVGGRQTTATGRSFDIDVDCLGRVERFLAKRMPG
                     ALGPAVLTKTCLYTLTPDRDFVVDRVPGHPGVIAALGAAHAYKFAALLGTRLAELALD
                     PQRAAPRADLALFSLERDALRAATNLAPAS"
BASE COUNT          726 a         1417 c         1618 g          724 t
ORIGIN      
        1 tcaggatgat gcacaggacg aaggtcggca ggacaaggaa gaagtccgcg attcgcatca
       61 tgaacgcgtc gaaccggccg ccgacgtagc cgctcaggat cccgatcacg gccccgaatg
      121 cgaccgtgat cacggtcgcc agcagcccga tgaccatcga gatgcgggtc gcatggaccg
      181 tcaggttcag gatcgaccgg cccaactcgt cggttccgaa cgggaacgtc cccgatggcg
      241 gctcgaggcg ggtcccgggg gcggtcaccg ccgtctgcag cggccccacg aagaagtccg
      301 gcgcgatcgc cagcacggtg aagaaggcaa ggatggccag gccggcgaaa ccattcgggc
      361 tgcgcaggag cttgcgggtc gccgcgagca tgccggtggc cggcgcggca gccaaggcga
      421 tcggcatcgc gggcgctgtc atcgtcgttc gcccgtcacg tccgcacccg cggatcgagg
      481 tagccgtaga cgacgtcggc gatgagattg gcgagcacga cggtcacgct gagcaggagg
      541 aacacgcctt gcaggaccgg gtagtcccgg cccgaaagcg catcgacggt gagcgtgccg
      601 aggccaggcc agttgaagac gacctcgact gtgatcgcgc cggcaaccac gtacccgagg
      661 ttgatcgcca ccagggtgac cgtcggcagg agcgcgttcg ggatcgcatg ggcgcgaagc
      721 acacggccgt ccccgatccc cttcgccttc gcggtggtga tgtagtcctc gctcagggtc
      781 tcgaccaccg acgagcgcat gaggatcgcg tactgcccga cgagcccgag cgacaccgtc
      841 gccagcggaa ggaagaggtg ccaggcgatg tccgacagct catcgagcgg gccgtcgtac
      901 accctgccga catcggtcat cccgtatgtc gggaaccacc cgaggccggt cgcgaagagc
      961 agcagcagcc ccatgcctaa caggaagtac ggcgtcgaat acaggatcag cgagatgccg
     1021 ttgccgatgt agtcgacgat gccgccccgt cgccagcccg agtacgcgcc cagcgcaagg
     1081 ccgaggacga tggcgaggat ctcgcccagc ccgaacagca acagggttgg tccgagccgg
     1141 gacccgagca cctcggtcac cggccgcccg cgcgacacga aggagaagcc gaggtcgccg
     1201 cgcaacgtgg agctcacgta ggcgacgaac tggtccggga agagcggctt gtcgaggccc
     1261 cagcgggcct tggtcgtctc gagcacctgc tgcgagatac ccgggatgcg gccgagcagg
     1321 atccgctcgg gcgagcccgg catcatccgg aacaggacga agttgaggca gatgatgaag
     1381 aagatcgtca ccgccgccgt cacgagacgg cgagcgatat agcggacgcc caccgatcag
     1441 cctgccccgg gtcggtcggc ggtgaacgtg tggcctgccc gagctacggg caggccacag
     1501 ctcattcctc ttccttggct gcgccgcgac ggcgcatgaa caagagcccg cccacgatga
     1561 cgaggatcac gatgaccccg ccgatcagga gcggcgtcga gtcgctggcg gtcggcgtcg
     1621 gtgtcggcgt gggcgtcgcc gctggcccgg cggtgacacc gggcggcacc ggcgttgccg
     1681 cgaccgttgg gccgggcgtc gggacggcgc tcgcgtcggt cagggccatg tagccggagt
     1741 agccgaaccc gaagaacggc gtacccgtgt ccggtggctg gttcacccaa cccccgaact
     1801 tgtcggtgcg gtatgcgtgc agctcgctgt cgtagtacag gacgtggtag caagccgcgt
     1861 cgtagaacag gttctgcatc tcggcgatcg ccgtcttgcg gacggtctca tcgagggcgt
     1921 gcagctgttc gttgaacagc tcgtcgtagc gcgcatcgga gtagaagcag tcgttgtagc
     1981 ccgatgggat gttgtccgtg gtgaaggtgc ccaggagtga cgtcgggtcg ggatcgccgc
     2041 cccagcccca catgtagaag tcccagttcg ccggaccgtc ggcatcgggc ccgtagagcg
     2101 ccttgatgag cgcgccctcc tcggtcaccg aggcgtccac gccgatgccg atctgcgcga
     2161 accagccctg gatgaactgg gcatcggcgg agtggtcttc ggagtccggc caggtcaggc
     2221 gcaggttgat cggcttgcct tccttgtcga cccggatgcc gtcagcgccc ttggcgtagc
     2281 cggcggcgtc caggaggctg ttcgctttcg ggatgtcgaa ggtgcgcggc gtcgtcggct
     2341 cgacgtgcca cttgacgtgg tacggcggga cgtgggtcga cccggcgacg ccgtggccat
     2401 tcaggacctt gtcgacgagc tcctggcgat cgatcgcgta gccgagcgcg tcgcggaact
     2461 tctggtcctc cagcgcggat gtcgagccgt ggtagccctc cttcgtgccg ccggtgttga
     2521 acgacaggta ggtgtagccg ttggcatagc cctcggagcg ggcgacgttc ggctcggtcg
     2581 cgagggcgtc gaactggttc gcgcccgtgc cgcgcacgta gtccagctgc ttgttcttga
     2641 gcgcttggac catcgcgtcg ttggtcgaga actgctccat gatgatcgcg tcggggacgc
     2701 ccttgttggt gccccagtag ttggggttgc ggtcgaaccg gacgaacttg cccggctcaa
     2761 actccgacac gacgtacggc ccggagccca cgaccggcag gtccttgttg ccgaagtagc
     2821 cgtcggcctc gaggttgccg atttcctcga gcgagtactt cgaccaaacg tgcttcggga
     2881 cgatcgggat gtaggtctgg gtgatcagcg tcgtcgggaa ctcggtgatg gcgatgaggt
     2941 tgttgttcgc gtcgcacttg gcactcgtga ggccggtgtt cgtcaggtac gggtcgagat
     3001 agccggagcc caggtagccg gtctccgttt ccgccgcgtc gaggaagagc ttgaaggtgt
     3061 attggacgtc cgcgcaggtc gcgggctcgc cgtcggacca cttcatgccg tcgcggatgt
     3121 ggaaggtgtg ggtcttcttg tcggcagacg agctccactc gtcggcgaag cccgggacgg
     3181 cctccaggtt cgggccgaag ctgacgagca gctcgtactg gatctggaag acctcgtagt
     3241 cggcgtaggt gatcgcctgg aacgggtcga tcgattcgag cttttggtcc gtgccgatcc
     3301 ggaggatcag ctcctcggtg ccggcctgtg cgccgcccgc ggacagcagg gtcggggcaa
     3361 ggagcatcag ggacgcgagg aaggctcggg catgtttcac gcgccgggac cgtgggccag
     3421 cgtcgctcga cgatcgcatg tagtcctcct caaccacgac gccgggtgcc gggcccatag
     3481 gctccgatcg gggtcccgtt cgggtggtgc tcggctaggt tcgcacgctg gggcggcccg
     3541 cgcaacgcga tccgtcgacg attcagctcg cgggcgcgag attcgtcgcg gcgcgaaggg
     3601 cgtcacgctc gagggagaac agcgcgaggt cggctcgcgg cgcggcccgt tgcgggtcga
     3661 gggcgagctc cgcgaggcgt gttccgagca gcgcggcgaa tttgtacgcg tgcgccgcgc
     3721 ccaaggcggc gatcacaccc gggtgtcccg ggacgcgatc gacgacgaaa tcgcggtccg
     3781 gcgtgagcgt gtacaggcag gtcttcgtga gcaccgcggg gccgagcgca cccggcatgc
     3841 gcttggccag gaagcgctcg acccgaccga ggcagtcgac gtcgatgtcg aacgagcggc
     3901 cggtggcggt cgtctggcgg ccaccgacgt cctcgccgac cttcggcccg gcggagcggt
     3961 acgtcgggaa gccgtagaag ctcggctggt cgagccagat ccagatcggg aagcgggcgg
     4021 gctcgaaggc ggcggcatcc tttggttcga accaggtgac ctgctcgcgg gtgatgctga
     4081 gcggcagctc gtggccaagg ggcgcgagga ggtcgttcgt ccaggcgtcg gtcgcgacga
     4141 ccacggcgcc cgtgctgaac gtctcgcctc cctcggtgac gacctcgatc tcattgccct
     4201 gatcgtggat ggccgtcacg cgctggcgct ccaggagcgt cgccccggcg gcccgggcaa
     4261 ggcgctgatg cgcggcgttg cctggttccg gatctgcaat cccggcatcg gcctggaaga
     4321 tcgcacgtgt attggggtcg agccgccatt gcggccagcg cctggcgacg tctgtggcgt
     4381 cgagccactc gaacgggacg ccgacctcgg tcatcgccgt cgcgtagtcg tcgatcgact
     4441 cggccgcgtt cggcgggcac aggtcgagcc cgccggtcat cgtca
//
LOCUS       DATBSW010000053         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1167898, whole genome shotgun sequence.
ACCESSION   DATBSW010000053 DATBSW010000000
VERSION     DATBSW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167898"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VIF63_01360"
     CDS             complement(<1..1497)
                     /locus_tag="VIF63_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8238056.1"
                     /translation="MQGRTDSGRRLIVLLIVFVVASSALVVRLGYWQLAKHDELVDSA
                     KAQIFYRAEVPSRRGQIYDRSGTVVLAASVMRDRLIVSAEHLDDAEQAEMVAFLTMQL
                     SLDPAGVATLQERLATGKPYLVIARDLAPEQSEVIETAAHAAGIDGISFESDSIRSYP
                     QDGGGPKSTLAAHLIGFVNREGDGQYGVEQFYQALLAGQPKVVEADRDASGKPMSETE
                     RTVEPGVPGVDIRLTIDAGLQLALEQEVMAARIANGAKSVSAVVMDPWTGEIYAEATY
                     PSYDANEYASVAGEDTSRFIDPVVSEVYEPGSVFKMLTVLAGFEQGTTTLQTVYRDTG
                     RMKLDKGRASISDSDGKAMGKMKLEDAIAYSRNVVAAKVALGLAPTTDKASTILHEVW
                     TRLGFGSRTGIDVAGETQGLVNDPGIEKWRQIDLANGSFGQGVAVSQIQLASAFAALV
                     NGGMRVQPHVVAGIGTSTVEAPTEGPLIDPGLSPRLSYLMKHVLESPWY"
     gene            complement(1510..1956)
                     /locus_tag="VIF63_01365"
     CDS             complement(1510..1956)
                     /locus_tag="VIF63_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238057.1"
                     /translation="MAVYQGARRRAPLPLGLDALAGKRDRDRERAIDGVSAGREKGRQ
                     SRAAGARRSATAGRRRDRPSSIGVALVAIMIVFSVAFLSLSQSVRVAATGYDIVRLVS
                     ERDRLEAIRADLLSDVQRLRSEPAIRKQALDAGLGQLGAPLLIPAR"
     gene            complement(1956..2144)
                     /locus_tag="VIF63_01370"
     CDS             complement(1956..2144)
                     /locus_tag="VIF63_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238058.1"
                     /translation="MSKRHSTNRRRTYGRRQHELHERIDRRTERRADRARAGNEPGLD
                     PVGYLDARSGEPPFGFAD"
     gene            complement(2195..>3027)
                     /gene="rsmH"
                     /locus_tag="VIF63_01375"
     CDS             complement(2195..>3027)
                     /gene="rsmH"
                     /locus_tag="VIF63_01375"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8238059.1"
                     /translation="LEASDPDGRLLGLDADGAAIARVRARLAPRFGPRLDLRQANFRE
                     LAVVAPEAGFDAIDGCLFDLGLSSFQLADAERGFGFRTGGPLDMRFDPDRGVPASDLI
                     ANLDAAELTALFRQYGEEPFAGRIARAIVETRRTTPIQTAEELAALIARVAPSRAPGR
                     RRVHPATRAFQALRIAVNEELDALQAGLAAAVDLLRPGGRLVVLSYHSLEDRIVKRFL
                     QAERRGCTCPTEVPVCVCGRKPRLRLLSPKGLVPTAAEISANPRARSARLRAAERMAA
                     "
BASE COUNT          416 a         1015 c         1158 g          438 t
ORIGIN      
        1 gtaccacggg ctctcgagga cgtgcttcat gaggtacgac aggcgggggc tgaggccggg
       61 atcgatgagc gggccctcgg tcggcgcctc gacggtgctg gtgccgatcc cggcgacgac
      121 gtgcggctgc acgcgcatgc cgccgttcac gagggcagca aacgccgacg cgagctggat
      181 ctgggagacc gcgacgccct gcccgaacga accgttggcg aggtcgatct ggcgccactt
      241 ctcgatgccg ggatcgttga ccaggccctg cgtctcgccg gcgacgtcga tgccggtccg
      301 gctcccgaag ccgagccggg tccagacctc gtgcaggatc gtcgacgcct tgtcggtggt
      361 cggcgcaagg ccgagtgcca ccttggcggc gaccacgttg cgcgagtacg cgatcgcgtc
      421 ctcgagcttc atcttgccca tggccttgcc gtcggagtcg gagatgctcg cgcggccctt
      481 gtccagcttc atccggccgg tgtcgcggta gacggtctgg agcgtggtcg tgccctgctc
      541 gaagccggca aggacggtca gcatcttgaa cacggagccc ggctcgtaca cctcggagac
      601 gaccgggtcg atgaaccgcg aggtgtcctc accggcgacg ctcgcgtact cgttcgcgtc
      661 gtacgacgga taggtcgcct cggcgtagat ctcgccggtc cagggatcca tcaccactgc
      721 agagacggac ttcgcgccgt tggcgatacg cgcggccatc acttcctgct cgagcgcgag
      781 ctggagcccg gcgtcgatgg tgaggcggat gtcgacgccg ggcacccccg gctcgaccgt
      841 gcgctcggtc tcggacatcg gcttgccgga cgcgtcgcgg tcggcctcga ccaccttcgg
      901 ctggccggcc aggagggcct gatagaactg ctcgacgccg tactggccgt cgccctcgcg
      961 gttcacgaac ccgatgaggt gcgcggcgag cgtgctcttc gggccgccgc cgtcctgcgg
     1021 gtagctccgg atcgaatcgg actcgaagct gatcccgtcg attcctgcgg cgtgcgccgc
     1081 ggtctcgatg acctccgatt gctcgggcgc gaggtcgcgc gcgatgacga ggtacggctt
     1141 gcccgtcgcg aggcgttcct ggagggtcgc gacgcccgcc gggtcgaggc tgagctgcat
     1201 cgtgaggaag gcgaccatct cggcctgctc ggcgtcgtcg aggtgctcgg cggacacgat
     1261 gagccggtcg cgcatcacgc tcgcggcgag gacgacggtg ccgcttcggt cgtagatctg
     1321 gccccgccgg ctcggcacct cggcgcggta gaagatctgc gccttggcgc tgtcgacgag
     1381 ctcgtcgtgc ttcgccagct gccagtagcc cagccggacg acgagcgcgc tcgacgcgac
     1441 cacgaacacg atcaggagca cgatgaggcg gcgccccgaa tcggttcgac cctgcatggc
     1501 tgtcgctcgc tagcgggcgg ggatgaggag gggcgcgccg agctggccga ggccggcatc
     1561 gagcgcctgc ttgcggatgg cgggctcact gcgcaatcgc tggacgtcgg acaagaggtc
     1621 ggcccggatg gcctcgaggc ggtcgcgctc ggagacgaga cgaacgatgt cgtagcccgt
     1681 cgcggcgacg cggacgctct ggctcagcga caggaacgcg acgctgaaca cgatcatgat
     1741 cgcgacgagg gcgacgccga tcgatgacgg ccggtcgcgg cggcgacccg cggtcgcgga
     1801 ccggcgcgcg ccggcggcgc ggctctggcg tcccttctcg cggcctgcgg acacgccgtc
     1861 gatggcccgt tcgcgatcgc ggtcacgctt cccggccagc gcgtcgaggc cgagcggcaa
     1921 cggggcgcgg cggcgggcgc cctggtagac cgccatcagt cggcgaagcc gaagggcggc
     1981 tcgcccgagc gcgcatcgag gtagccaacg ggatcgagcc ccggctcgtt gccggcgcgg
     2041 gcgcggtcgg cgcggcgctc ggtgcggcgg tcgatccgct cgtgcagctc gtgctgccgg
     2101 cggccatacg tgcgacgacg gttcgtggag tggcgcttgc tcattggggg ctcctccttc
     2161 ctctcattcc gactcggact gcgctccagg gcgactaggc ggccatgcgt tctgcggcac
     2221 gcaggcgcgc gctgcgggcg cgcgggttgg cgctgatctc ggcggcggtg gggacgaggc
     2281 ccttcggtga caggagtcgg aggcggggct tgcggccgca tacgcacacc ggcacctcgg
     2341 tcgggcaggt gcagccccgc cgctcggcct ggaggaaccg cttgacgatc cggtcctcca
     2401 gggagtggta gctcaggacc acgagccggc cgccgggccg gagcaggtcg acggcggcgg
     2461 cgaggccggc ctggagcgcg tcgagctcct cgttgaccgc gattcgcagc gcctggaaag
     2521 cgcgggtcgc ggggtggacc cggcgacgac cgggggcgcg cgacggcgcg acacgggcga
     2581 tcagcgcggc gagctcctcg gcggtctgga tcggggtcgt gcggcgggtc tcgacgatgg
     2641 cgcgggcgat gcggccggcg aacggctcct cgccgtactg ccggaagagc gccgtcagct
     2701 cggcggcatc gaggttggcg atgaggtcgg aggcgggcac gccgcgatcg gggtcgaagc
     2761 gcatgtccaa ggggccgccg gtccggaacc cgaagccgcg ctcggcgtcg gcgagctgga
     2821 agctcgacag cccgaggtcg aacaggcagc cgtcgatcgc gtcgaagccc gcctcgggcg
     2881 cgacaacggc cagctcgcgg aagttcgcct ggcggaggtc caggcgaggc ccgaaccggg
     2941 gcgcgagccg ggctcgcact ctggcgatgg ccgccccatc ggcatccagg ccgaggaggc
     3001 ggccatcagg gtcggaagct tcgagga
//
LOCUS       DATBSW010000054         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_568562, whole genome shotgun sequence.
ACCESSION   DATBSW010000054 DATBSW010000000
VERSION     DATBSW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568562"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..990)
                     /locus_tag="VIF63_01380"
     CDS             complement(175..990)
                     /locus_tag="VIF63_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238060.1"
                     /translation="MRIFVAGLRKLVGRPASFVSVGLLVGLLGLIILASATVGQNGDD
                     PGDGRAASLLLTFPGAYQFVLSFMFGLGGLVAVCFAAAVAGSEWSWGTLKSAVARGES
                     RIRYTLGTFAAIALMIGVGLLVTFVLGVALVVLGATIAGISTAGITDADTLARLPSMA
                     ARGWFAVVEEAALGFAIATLARSQLAGIGAGIAFYFGELFATVFLPDIVKYMPFAVAN
                     ASIDTGSGGGGFGGGGGGVPSLEPDTALALVAAWLVGSLIVAVLFTERAEITG"
     gene            complement(987..1985)
                     /locus_tag="VIF63_01385"
     CDS             complement(987..1985)
                     /locus_tag="VIF63_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8238061.1"
                     /translation="MTSPGTLALATRGLRKSYGSRLALDGLDLSVPQGVVYGFLGPNG
                     AGKTTTMRLLTGLLHPDAGTIELLGSPFRRGDRKRLFQVGALVESPSFYPYLDGRQNL
                     KALAASGAAVPAGRIEELLELVGLRDRAKDKVAGYSLGMKQRLGIAGALLSDPRLLLL
                     DEPANGLDPAGIVAMRETLRRLAASGKTVFVSSHLLAEVQVLADVVGIIARGKLVREG
                     SLESMLGDQGVVRVRISKAAVATARAALEPIAPVEARTNDEAGEQWLAVHVEPSRAAE
                     VNQRLAEAGVYASGLESGTDLELLFLELTGGDAATSHEGTFGSIGTPGGAGTGAAA"
     gene            2003..2467
                     /locus_tag="VIF63_01390"
     CDS             2003..2467
                     /locus_tag="VIF63_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238062.1"
                     /translation="MDLPAPRASGFWRRALPVMYALIKALDVVIRPSWRASGVGLRNV
                     VELRVAGRRTGRYRSVLLTLLTDGDQWFLGHPNGHVAWTLNLEAAGTADVAFRGLPPV
                     AVSARVLAPGELRDRAIRATVQQPFPANVVYRLARAHIRAVGVYFAMDPISA"
     gene            2486..>2895
                     /locus_tag="VIF63_01395"
     CDS             2486..>2895
                     /locus_tag="VIF63_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY8238063.1"
                     /translation="MTERRSRFQLPSRRTATPSTQPAQPAPEVSMEARPEDITAPVEQ
                     SVVDAAIYVDGARVDSPETVAGTVQRLRERPGAMAWIGMFRPTEAQLRPVAEEFGLHE
                     VAVEDAVVAHQRPKSERYGETLFVVLRAATYLDAL"
BASE COUNT          427 a         1045 c         1032 g          391 t
ORIGIN      
        1 gttgggtcag ggtagtcggt gcagccgacg tgatagatgc ccttggcgcg tggcagaagc
       61 gtccgtttcg gcggatacct tcaccgggac agcatcggag cggctccttc gggagccgct
      121 ccgaactaat tccggccgga attcgcgtgg ttcgcgcggc tcgcgcggag ccagctagcc
      181 cgtgatctcc gcccgttccg tgaacagcac cgccacgatc agcgagccga cgagccaggc
      241 ggccacgaga gcgagcgcgg tgtcgggctc gagcgatggc acgccaccgc cgccgccccc
      301 gaagcccccg ccgccgctcc cagtgtcgat cgacgcgttt gcgaccgcga acggcatgta
      361 cttgacgatg tccggcagga agaccgtggc gaacagctcg ccgaagtaga acgcgatgcc
      421 ggcgccgatc ccggccagct ggcttcgggc aagggtcgcg atggcaaagc cgagcgccgc
      481 ctcctccacg accgcgaacc agccgcgcgc cgccatggac ggcagccgcg cgagggtgtc
      541 ggcgtcggtg atgccggccg tcgagatgcc cgcgatcgtt gcgccgagaa ccacgagggc
      601 cacgccgagg acgaaggtga cgagcagccc gacgccgatc atcagcgcga tcgcggcaaa
      661 ggtgccgagc gtgtagcgga ttcggctctc gccccgcgcc acggcgctct tgagcgtgcc
      721 ccagctccac tcggagccgg cgaccgcggc ggcgaaacag accgcgacga gcccgccaag
      781 cccgaacatg aacgacagga cgaactggta ggcgccgggg aacgtgagga gcaggctcgc
      841 ggcgcggccg tcgcccgggt cgtcaccgtt ctggccgacc gtggccgacg cgaggatgat
      901 caggccgagc agccccacga gcaggccgac cgacacgaac gacgccggcc ggccgacgag
      961 cttgcgcagt ccggcgacga agatcctcat gcggccgctc cggtgccggc gccgccgggc
     1021 gtcccgatcg agccgaacgt gccctcgtgc gatgtcgccg catcgccgcc ggtcagctcc
     1081 aggaacagca gctcgagatc cgtgccggac tcgaggccgg aggcgtagac gccggcctcg
     1141 gcaaggcgct ggttgacctc ggcggcgcgg ctcggctcga catgcacggc gagccactgt
     1201 tcgccggcct cgtcgttcgt gcgggcctcg accggcgcga tcggttcgag cgccgcccgc
     1261 gcggtcgcca cggcggcctt cgagattcgc acccgaacga cgccctggtc gccgagcatc
     1321 gactcgaggc tgccttcgcg gacgagcttg ccccgggcga tgatcccgac gacgtccgcc
     1381 agcacctgca cctcggcgag caggtggctc gacacgaaca ccgtcttgcc gctcgccgca
     1441 agccgccgca gcgtctcgcg catggcgacg atgcccgccg ggtcgaggcc attggccggc
     1501 tcgtcgagga ggagcaggcg cgggtcgctg aggagcgcgc cggcgatccc gagccgctgc
     1561 ttcatgccca gcgagtagcc ggcgaccttg tccttggccc gatcgcgcag cccgacgagc
     1621 tcgagcagct cctcgatccg ccctgccggc acggccgcgc cggatgcggc caatgccttc
     1681 aggttctggc gcccgtcgag gtacgggtag aacgacggcg attccaccaa cgccccgacc
     1741 tggaacaggc gcttccgatc gccgcgccgg aacggcgacc cgagcagctc gatcgtaccg
     1801 gcgtcggggt ggagcagccc ggtgagcagg cgcatcgtgg tcgtcttacc ggcgccgttc
     1861 gggccgagga agccgtagac cacgccctgc ggcacggaca ggtcgagccc gtcgagggcg
     1921 agccgcgagc cgtagctctt gcgcaggccg cgtgttgcca gggccagggt tcccggggaa
     1981 gtcatgggag tatcttgcca gcgtggacct tcccgcgccg cgggcctcgg gtttctggcg
     2041 ccgcgccctg cccgtcatgt acgcgctgat caaggcgctg gacgtggtga tccggccttc
     2101 gtggcgggcc tcgggcgttg ggcttcgaaa cgtcgtcgag cttcgggtcg cgggacggcg
     2161 aacaggccgc taccggagcg tcctgctgac cctgctcacc gacggtgacc agtggttcct
     2221 cggccacccg aacggccatg tcgcgtggac gctcaacctg gaggctgcgg gcacggccga
     2281 cgtcgccttc cgcggcctgc cgccggtcgc ggtgtctgcg cgggtcctcg cgccgggcga
     2341 gctgcgcgac cgggcgatcc gcgcgacggt ccagcagccg tttccggcga atgtcgtcta
     2401 ccggctggcg cgcgcacaca tccgcgccgt cggcgtctac ttcgccatgg acccgatctc
     2461 cgcctgagcg cgtagccttg ccgggatgac tgagcgccgg tcccgtttcc agctgccgag
     2521 ccggcgtacc gccacaccct cgacacagcc tgcccagccg gcgcccgagg tgtcgatgga
     2581 ggcgcggccc gaggacatca ccgcgccggt cgagcagagc gtggtggacg cggcgatcta
     2641 cgtggacggg gcccgcgtcg actcgcccga gactgtcgcc gggaccgtcc agcgccttcg
     2701 agagcgaccc ggcgcgatgg cctggatcgg gatgttccgg ccgaccgagg cgcagctccg
     2761 gcccgtcgcc gaggagttcg ggctgcacga ggtcgcagtc gaggacgcgg tcgtcgccca
     2821 ccagcggccc aagagcgaac gctacgggga gaccctgttc gtcgtgctcc gggcggcgac
     2881 ctacctcgac gccct
//
LOCUS       DATBSW010000055         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1097738, whole genome shotgun sequence.
ACCESSION   DATBSW010000055 DATBSW010000000
VERSION     DATBSW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097738"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1461)
                     /gene="glyA"
                     /locus_tag="VIF63_01400"
     CDS             complement(124..1461)
                     /gene="glyA"
                     /locus_tag="VIF63_01400"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8238064.1"
                     /translation="MTITTESRLGWAPLAEVDPELWHAMVQERNRQRDKIELIASENY
                     TFAAVMEAQGSWLTNKYAEGLPGKRYYGGCEYVDIAETLAQERALALFPGAEHVNVQP
                     HSGAQANMSAYFSVLVPGDRILGMNLAHGGHLTHGSPVNFSGRLYEVHAYGVDQGTER
                     IDYDELAAKAREVRPKVIVAGASAYPRIIDFPRMAEIAHGVGALLFVDMAHIAGLVAA
                     GVHPSPFPHADIVTTTTHKTLRGGRGGLIFSRTDLPAEVDKADFPMVKTTLAAQIDKA
                     VFPGVQGGPLMHVVAGKAVSLKLAAGEPFKRDQRRTIENAAVLADALAERGARIVSGG
                     TDNHLMLVDVTPLGVTGKEAEALLDDVGITVNKNAIPFDPLPPNTASGIRLGTPATTT
                     RGFGPDEMRTIARIFTEAIAQRDDPAASARLRAEAGEIASRFPVPGLFAEDAG"
     gene            1578..1967
                     /locus_tag="VIF63_01405"
     CDS             1578..1967
                     /locus_tag="VIF63_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238065.1"
                     /translation="MPALPGILGGNKPFRIKGAKDMTIASMRSAVAGGARIVIFPYAE
                     SWLVVSFKRTSEPTLVPAGASRLSVALVPILHSLLFGWWGFPWGPIFTVQTIWQSARG
                     GIDVTDAVMPALEQDYADRPLDLLPAS"
     gene            complement(1928..2596)
                     /locus_tag="VIF63_01410"
     CDS             complement(1928..2596)
                     /locus_tag="VIF63_01410"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8238066.1"
                     /translation="MERAQDGEAGRAAAIRVLRAGGVIAIPTDTVYGIAVALETPGGI
                     ERLFAAKSRPPDKAIALLLADAEQAAEIGVITAAAATLANAFWPGGLTLVVPRRTDRE
                     LPAALTGGALAPGAIPTVGLRVPDHEAPRALARALGPLPTTSANRSGEPELSDAAEIE
                     ATLGDALDLILDGGPSGGGPASTVVDCTGEAPLIVREGAIPATAIERVVAQEAGSRSS
                     GRSA"
     gene            complement(2614..3516)
                     /locus_tag="VIF63_01415"
     CDS             complement(2614..3516)
                     /locus_tag="VIF63_01415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEY8238067.1"
                     /translation="MSTVRELLDVGIARLRDSGSESARLDAELLLGFALDTDRTAVIA
                     HTDAPVGDGAAARFEAALDRRAAGEPVAYIRGVKEFHGLAFGVDPRALIPRPETELLV
                     DLAELEIVRRLVSAPRARGAAQIQVIDVGVGSGAVVVSVAVLMRRRKMLDEVAFTATD
                     VSEDALQLARENAVAHAVGDRITFEAVDLLPGTQVQTWDVVLANLPYVRTDAIPGLPI
                     AASFEPALALDGGPDGLRVIDRLLAQLPGALARDGVALLEIGGDQAVEMRALAAERLP
                     GWSCEIELDLGRLPRVAVLCRGAS"
     gene            complement(3539..>4120)
                     /locus_tag="VIF63_01420"
     CDS             complement(3539..>4120)
                     /locus_tag="VIF63_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY8238068.1"
                     /translation="GAYSRLKFESGTHRVQRVPATESSGRIHTSTATVVVMPEAEETE
                     IEIDEERDLRIDVKRASGPGGQSVNTTDSAVRVTHLPTGLVVEIQDEKSQHKNKAKAI
                     AVLRARLYDLERQRQRESDSATRKSMIGAGDRSDKIRTYNYPQDRVTDHRVNEDLSNL
                     PRVLGGELDKLIDTLITTDQAERLSALVESTPD"
BASE COUNT          553 a         1441 c         1497 g          629 t
ORIGIN      
        1 cgatgatgcg caggttgcgc acgattcgaa ggatcacggg ggtcgcgagg aaggcgacga
       61 gcgccgcggc gatgaacgcg ccggcgatga tgggcagggc cgccaggttg gcggccggga
      121 agctcacccg gcgtcctcgg caaagaggcc gggcaccggg aagcggctcg cgatctcgcc
      181 cgcctcggcg cggaggcgcg cgctggctgc cggatcgtcg cgctgggcga tggcctcggt
      241 gaagatccgg gcaatcgtgc gcatctcgtc gggaccgaag ccgcgcgtcg tcgtcgccgg
      301 cgtcccgagg cggatccccg aggccgtgtt cggcggcagg ggatcgaacg ggatcgcgtt
      361 cttgttcacc gtgatgccga cgtcgtcgag gagcgcctcg gcctccttgc cggtcacccc
      421 gagcggcgtc acgtcgacga gcatcaggtg gttgtccgtg ccgccggaca cgatccgcgc
      481 cccgcgctcg gcgagggcat ccgcgagcac cgccgcgttt tcgatcgtgc ggcgctgatc
      541 gcgcttgaac ggctcgcccg cggcgagctt gaggctgacg gccttgcccg cgacgacgtg
      601 catcagcggg ccgccctgga cgcccgggaa cacggccttg tcgatctggg cggcgagcgt
      661 cgtcttgacc atcgggaagt cggccttgtc gacttcggcg ggcaggtcgg tccggctgaa
      721 gatcaggccg ccgcgaccgc cgcgcagcgt cttgtgggtc gtcgtcgtga cgatgtcggc
      781 gtgcgggaac gggctcggat ggacgcctgc ggccacgagg ccggcgatgt gggccatgtc
      841 cacgaacagg agcgccccca caccgtgggc gatctcggcc atccgcggga aatcgatgat
      901 gcgcgggtag gcggacgcgc cggccacgat caccttcggc cgaacttccc tggcctttgc
      961 ggcgagctcg tcgtagtcga tgcgctcggt cccctggtcg acgccgtacg cgtggacctc
     1021 gtacaaccgt cccgagaagt tcaccggcga gccgtgggtg aggtggccgc cgtgggcgag
     1081 gttcatgccc aggatccggt cgcctggcac gaggacgctg aagtacgcgg acatgttggc
     1141 ctgggcgccc gagtggggct gcacgttcac gtgctcggcg ccggggaaga gggccagcgc
     1201 ccgctcctgg gcgagggtct cggcgatgtc cacgtactcg cagccaccgt agtagcgctt
     1261 gccgggcagg ccctcggcgt acttgttcgt gagccacgag ccctgggctt ccatgaccgc
     1321 ggcgaacgtg tagttctcgc tggcgatcag ctcgatcttg tcgcgctgcc ggttccgctc
     1381 ctggaccatg gcgtgccaca gctcgggatc gacctcggcc aggggagccc agccgaggcg
     1441 agattcggtc gtgatcgtca tctgacggtg ttcgcccctg gctgggcgga tgcccgctgc
     1501 caacgcatgg tgacagcctg attcgccgca cggtatcgtc ccacatcgac cgggccatcg
     1561 gcgacttgga ggcgggcatg ccggctttgc cagggatcct cggcggcaac aagccgttcc
     1621 ggatcaaggg cgcgaaggac atgacgatcg cgagcatgcg ttccgcggtc gccggcggcg
     1681 cccggatcgt catcttcccc tatgccgaat cgtggctcgt cgtgtcgttc aagcgcacct
     1741 ccgagccgac tctcgtgccg gccggggcgt cgcgcctgtc ggtcgccctc gtgccgatcc
     1801 tgcactcgct gctcttcggc tggtggggct tcccgtgggg cccgatcttc accgtccaga
     1861 ccatctggca gtccgctcgc ggcggcatcg acgtcacgga cgcggtgatg cccgccctcg
     1921 agcaggacta cgccgatcgc ccgctggacc tgctgccggc ctcctgagcg accacgcgct
     1981 cgatcgccgt ggccgggatc gccccctcgc gcacgatcaa gggtgcctcg ccggtgcagt
     2041 cgacgacggt cgacgccggg ccgccgcctg acggcccgcc atcgaggatc aggtcgagcg
     2101 cgtcgccgag cgtcgcctcg atctccgccg cgtcgctcag ctcgggctcg ccggaacgat
     2161 tcgcggaggt ggtcgggagc ggcccgagcg cgcgggccag ggcgcgcggc gcctcgtgat
     2221 ccgggacgcg aaggccgacc gtcgggatcg cgccgggggc aagggcgccg ccggtcaatg
     2281 ccgccggtag ctcgcgatcc gttcggcggg gcacgacgag ggtcagccct cccggccaga
     2341 acgcgttcgc gagggtcgcc gcggccgccg tgatcacccc gatctcggcc gcctgctcgg
     2401 cgtccgccag gaggagcgcg atcgccttgt ccggcggtcg cgactttgcc gcgaacagac
     2461 gctcgatgcc gccgggggtc tcgagggcca ccgcgatgcc gtagacggtg tcggtcggaa
     2521 tggcaatgac gccgcccgct cggagcaccc ggatcgcggc ggctcgcccg gcttcgccgt
     2581 cctgggcccg ctccaccacg tctcgcgccg ggctcagctc gcgccgcggc acaggacggc
     2641 gacgcgaggc agccggccga ggtccagctc gatctcgcac gaccagccgg gcagccgctc
     2701 ggctgcgagg gcgcgcatct cgaccgcctg gtcgccgccg atctcgagga gcgcgacacc
     2761 gtcacgcgcg agcgcgccgg gcagctgcgc gagcagccgg tcgatgaccc ggagcccatc
     2821 cggcccacca tcgagggcca gtgccggctc gaacgacgcg gcgatcggca gcccggggat
     2881 cgcgtcggtg cggacgtacg gcaggttggc gagcacgaca tcccacgtct ggacctgcgt
     2941 gccgggaagc agatccacgg cctcgaacgt gatccggtcg ccgaccgcgt gcgccaccgc
     3001 gttctcgcgc gcgagctgca acgcatcctc ggacacgtcc gtcgcggtga aggcgacctc
     3061 gtcgagcatc ttccgtcggc gcatgaggac ggcaacgctg acgacgacgg cgccgctgcc
     3121 gacaccgacg tcgatgacct ggatctgggc ggccccgcgc gcccgtggcg cggagaccag
     3181 ccggcgcacg atctcgagct ccgcgaggtc gacgagcagc tcggtctcgg gtcgcgggat
     3241 caacgcccgc gggtcgacgc cgaaggccag cccgtggaac tccttgacac cgcggatgta
     3301 cgcgaccggc tcaccggcgg cgcggcggtc gagggccgcc tcgaagcgcg ccgccgcacc
     3361 gtcgccgacc ggcgcatccg tatgcgcgat gacggcggtt cgatcggtgt cgagggcgaa
     3421 gccgagcagc agctccgcgt cgagtcgggc gctttcggag cccgaatcac gaagtcgggc
     3481 gattccgacg tccagcagct cacggacggt cgacatggat ccccgctccc ccgctcgatc
     3541 agtccggcgt cgactcgacg agcgccgaca gtcgctcagc ctgatccgtc gtgatgagcg
     3601 tgtcgatcag cttgtcgagc tcgccgccga ggacgcgggg caggttcgag aggtcctcgt
     3661 tgacccggtg gtcggtgacc cggtcctgcg ggtagttata ggtccggatc ttgtccgagc
     3721 gatcgcccgc gccgatcatc gacttgcgcg tcgccgaatc ggactcgcgc tggcgctgcc
     3781 gctccaggtc gtacaggcgg gcccgcagga cggcgatcgc cttggccttg ttcttgtgct
     3841 ggctcttctc gtcctggatc tcgacgacga ggcccgtcgg caggtgggtg acgcggaccg
     3901 cggagtccgt cgtgttgacc gactggccgc ccgggccgga cgcgcgcttg acgtcgatcc
     3961 ggaggtcgcg ctcctcgtcg atctcgatct cggtctcctc ggcctcgggc atgaccacga
     4021 cggtcgcggt cgaggtgtgg atccggccgg atgactcggt ggcgggcacg cgctggaccc
     4081 gatgggtgcc gctctcgaac ttgaggcgcg agtaggcccc
//
LOCUS       DATBSW010000056         4594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_145527, whole genome shotgun sequence.
ACCESSION   DATBSW010000056 DATBSW010000000
VERSION     DATBSW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145527"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..634
                     /locus_tag="VIF63_01425"
     CDS             197..634
                     /locus_tag="VIF63_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238069.1"
                     /translation="MHRRPVGWSRRLAAIGALVALAGCFLPWWTFGGRPGEVSALSGN
                     AFEGFGILVFAACIATLALVTLPYASERPVSADRWTSYALIAAAGWIGFGYRLVELLG
                     SPAFTFAEPAEIVLRMPGLWLVAIGLIILARATYEMLQEPRLR"
     gene            complement(643..1317)
                     /locus_tag="VIF63_01430"
     CDS             complement(643..1317)
                     /locus_tag="VIF63_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238070.1"
                     /translation="MVDEGPREVHVRIEDPGSRRAVRIAWIGAALIFVAMAKPWPQPA
                     PPEHIANQIARATPQVSAEPGLAVIEPCAGSWWSVEVDEAAESVARVWVLTDAVEATG
                     PLDARIRFVTVAAQQVRGLGFCPPFHDVPTRGSVTFFRLDPAPTPFRTTPVQMPQLRE
                     VAANTLYRPASTDGQGASGPGPPWAAGRYVMRIDGSDGYERWLGLDLRLVTFPARPLP
                     SAGPAV"
     gene            complement(1317..2162)
                     /locus_tag="VIF63_01435"
     CDS             complement(1317..2162)
                     /locus_tag="VIF63_01435"
                     /inference="COORDINATES: protein motif:HMM:NF020927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HEY8238071.1"
                     /translation="MNGKEARARLHRSVAAGQLLVGAGAGTGLSAKCAEAGGADLIVI
                     YNSGRFRMAGRGSLAGMLPYGDANAIVLDMARDVLPVVRDTPVLAGVCGTDPFRQMSV
                     FLAEVVRLGFAGVQNFPTVGLIDGVFRQGLEETGMGFGLEVDMIAEARRLDLLTAAYV
                     FDADQATQMTDAGADIVVAHMGLTTKGSIGAQTSLTLDESVTRVQQIHDATRQASKDT
                     LVFCHGGPIAEPDDVRYILDRTDGIAGFFGASSIERLPTELAITETVRQFKGIATGAG
                     SPPGT"
     gene            complement(2159..3466)
                     /locus_tag="VIF63_01440"
     CDS             complement(2159..3466)
                     /locus_tag="VIF63_01440"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEY8238072.1"
                     /translation="MTSVVILGTLDTKGAEIAFLADRIRERGGSVRVIDGGVMQSSAT
                     AWTFPSDVLVSDSDAVAKLGGSSRAALAAAGDRSAAMAVMRAGAVKEVDFLFGHHLLD
                     AVIAIGGSGGASFAAAAMQSLPVGIPKLIVSTMAAGDTRPYVGESDIAMLYPVVDIAG
                     LNRLSRQVLTNAAAAIAAMAEVAAATRPHGELQAALRRVSEDKPLIGATMFGVTTPCV
                     TAARETLEELGYEVLVFHATGTGGRALEGLVRSGYFAGVLDVTTTELADELVGGVLSA
                     GPDRLTAAGRLGIPQVVSLGALDMVNFGPRDTVPAQFRDRNLYEHNASVTLMRTSVEE
                     CRELGRILARKLNAATGPTALFIPRGGISMIDVPGGPFHDATADSALIDALLADLDPR
                     IEVVDRPVAINDESFGPAMARRLHELLQANPDPRMAALRAQSQ"
     gene            complement(3463..>4594)
                     /locus_tag="VIF63_01445"
     CDS             complement(3463..>4594)
                     /locus_tag="VIF63_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8238073.1"
                     /translation="TRRSTRPDRGPRPTVQRFIHVGTGGWGRMWSRTFLPHVIRLGLA
                     EPVAAVDMSEANLAIAREGYGIPEDRCYTDAARAFSERGAEADFAVVVVPPAHHEAII
                     DLALAHGLDILCEKPVADTMAASARIHAKVTRAGRKMAITMSHRFDRDKQTLERLVRS
                     GAYGPVSYIVGRLALNNRKHGDWGDFRHRIKDPLLIEAAVHQFGIIRGLAGANAKSIY
                     ANGWNPEWGEYAGDSTALAVIEMENGVRAAYEGSKTNASTMSPWDEEYWRVECRDGTL
                     ELDRRSLRLLVGGPNDLPTSTPIELDGDADDAWMNSLLAEQFVRWLDGGPAPETRLED
                     SIHDTALTMAAIESAHTGQVVDVAAYTAEHLAAATEALGAPR"
BASE COUNT          665 a         1618 c         1610 g          701 t
ORIGIN      
        1 atctgcacgc tcctcgacaa gcccgcgcgc cggctcgtcg agatcgaggt cgactacacc
       61 ggcttcacca tcccggacca gttcgtcgtg ggctacgggc tcgactacgg cgagctgtat
      121 cgaaacctgc gcttcgtggg cgtcctccga cccgaggtct acagcgaagg gtgaccgcgg
      181 agaatcgggc gtgaccatgc atcgccgtcc cgtcggatgg tcgcgccggc tggcagcgat
      241 cggtgcgctc gtcgcattgg ctgggtgctt cctgccgtgg tggacgttcg gcggccgccc
      301 gggcgaggtg agcgcgctca gcggcaatgc gttcgagggg ttcgggatcc tcgtcttcgc
      361 ggcgtgcatc gcgacgctcg ccctcgtcac cctgccgtac gcatccgagc gccccgtgtc
      421 cgccgatcgc tggacgagct acgcgctgat cgctgctgcg ggctggatcg gcttcggcta
      481 ccgcctggtg gaactgctcg gctcgcccgc cttcaccttc gccgagccgg cagagatcgt
      541 gctgcgcatg cccggcctgt ggctcgtcgc gatcgggctg atcatcctcg ctcgcgccac
      601 ctacgagatg ctccaggagc cgcggctccg ctgatccgag ggtcagaccg ccgggccggc
      661 ggacggcagc ggccgagccg ggaatgtcac gagtcgaagg tcaaggccca gccagcgctc
      721 gtagccgtcc gacccgtcga tccgcatgac atacctgccg gcggcccatg gcggccctgg
      781 tcccgacgcg ccttgcccgt cggtgctcgc cggccggtac agcgtgttgg ccgcgacctc
      841 cctgagctgg ggcatctgga ccggcgtcgt cctgaacggc gtcggcgcgg gatcgaggcg
      901 aaagaacgtc acggagccgc gcgtcggcac atcgtggaat ggcgggcaga accccaggcc
      961 gcggacctgc tgggcggcga cggtcacgaa tcggatccgc gcatcgagcg gcccggtcgc
     1021 ctcgacggcg tcggtcagca cccagacccg ggcaacagac tcggccgcct catcgacctc
     1081 gaccgaccac cagctgcccg cacagggttc gatcaccgcg agtccgggtt cggcgctgac
     1141 ctgtggcgtc gcgcgggcga tctggttcgc gatgtgctcc gggggagcag gctgcggcca
     1201 cggcttggcc atagcgacga agatcaacgc cgcaccgatc cacgcgatcc gtaccgcccg
     1261 gcggctcccg gggtcctcga tgcgaacgtg gacctcgcgc ggcccctcgt cgaccatcag
     1321 gtgcccggcg gggatcccgc gccggtggcg atgcccttga actggcggac cgtctcggtg
     1381 atcgcgagct cggtcggcag gcgctcgatg ctcgaggcgc cgaagaagcc ggcgatgcca
     1441 tcggttcgat cgaggatgta gcgcacgtcg tcgggctcgg cgatcgggcc gccgtggcag
     1501 aagaccaggg tgtctttcga cgcttggcgt gtcgcgtcgt ggatctgctg gacgcgggtg
     1561 acgctctcgt cgagggtcag cgacgtctgc gccccgatcg aacccttcgt ggtcaggccc
     1621 atgtgggcca caacgatgtc cgcgccggcg tcggtcatct gtgtcgcctg gtcggcgtcg
     1681 aagacatagg cggcggtcag gaggtcgagg cggcgggcct cagcaatcat gtcgacctcg
     1741 aggccgaagc ccatcccggt ctcctcgagg ccctggcgga acacgccgtc gatcagcccg
     1801 accgtcggga agttctggac gccggcgaag ccgagccgaa cgacctccgc gaggaacacg
     1861 gacatctggc ggaacggatc ggtgccgcac acgcccgcga gcacgggcgt gtcgcgcacg
     1921 acgggcagca cgtcgcgtgc catgtcgagg acgatcgcgt tggcgtcgcc gtacggcagc
     1981 atgcccgcga gcgagccccg gccggccatc cggaaccgcc ccgagttgta gatcacgatc
     2041 agatccgcgc cgccggcctc ggcgcatttc gccgagagcc ctgtcccggc gccggcgccc
     2101 accagcagct ggccagccgc caccgagcgg tgcaggcggg ctcgcgcctc cttgccgttc
     2161 attgcgattg cgctcggagg gcggccatgc gcgggtcggg gttcgcctgg aggagctcgt
     2221 ggagccgcct cgccatcgcc ggcccgaagg attcgtcatt gatggcgacc gggcgatcga
     2281 cgacctcgat gcgcgggtcc aggtccgcga ggagggcgtc gatcagcgcg ctgtccgcgg
     2341 tcgcgtcgtg gaacggaccg ccgggcacgt cgatcatcga gatgccaccg cgcgggatga
     2401 acagcgcggt cggtcccgtt gctgcattca gcttgcgcgc gaggattcgg ccgagctccc
     2461 ggcactcctc gaccgacgtc cgcatcagcg tcaccgacgc attgtgctcg tacaggtttc
     2521 gatcgcgaaa ctgggccggg accgtgtcgc gcggcccgaa gttgaccatg tcgagcgcgc
     2581 ccagcgacac gacctgggga atgccgagcc gccccgcagc ggtcagccga tccggacccg
     2641 cggacaggac gcccccaacg agctcgtccg caagctcggt cgtggtgacg tcgaggaccc
     2701 cggcgaaata gccactgcga accaagccct caagtgcccg accgccggtc cccgtcgcat
     2761 ggaagaccag aacctcgtac ccgagctcct cgagcgtctc gcgcgccgca gtgacgcaag
     2821 gtgtggtgac accgaacatg gtggctccga tgagcggctt gtcttcgctc acccgccgca
     2881 gcgcagcttg cagctccccg tgcggtcgcg tggccgcagc aacctcggcc atggccgcga
     2941 tggccgccgc ggcgttcgtg agaacctggc gcgacaagcg attgaggccg gcaatgtcga
     3001 ccacgggata gagcatcgcg atgtcgcttt cgcccacgta gggccgggtg tcaccggccg
     3061 ccatcgtcga gacgatgagc ttcgggatgc cgaccggaag cgactgcatt gccgcggcgg
     3121 cgaaagaggc tccgccactg cctccgatcg cgatcacggc gtccagaagg tggtgtccga
     3181 acaggaagtc gacctccttg acggccccgg cccgcatcac ggccatggcc gcggaacggt
     3241 cgcccgccgc tgcgagcgca gcgcggctgc tgccgccgag cttcgcaacg gcgtccgagt
     3301 cggagacgag cacgtcactc gggaatgtcc aggccgtggc cgaggattgc atgacgccgc
     3361 catcgatgac gcggaccgag ccgccgcgct cacgaatgcg atccgcgagg aacgcgatct
     3421 ccgcgccctt cgtgtcgagc gtccccagga tcacgaccga ggtcaccgcg gcgctccgag
     3481 cgcttcggtg gccgcggcga ggtgctcggc ggtgtaggcg gcgacgtcga caacctggcc
     3541 ggtgtgggcg ctctcgatgg cggccatcgt cagtgccgtg tcgtggatcg agtcctcgag
     3601 gcgggtctcc ggcgccgggc cgccatcgag ccagcgcacg aactgctcgg cgagcagcga
     3661 gttcatccag gcgtcgtccg cgtcgccgtc cagctcgatc ggggtcgagg tcgggagatc
     3721 gttcgggccg ccgacgagga gccgcagcga gcgccggtcc agctcgagcg tcccgtcgcg
     3781 gcactcgacc cgccagtact cctcgtccca cgggctcatc gtcgaggcgt tcgtcttcga
     3841 gccctcgtac gcggcgcgca cgccgttctc catctcgatc accgccagcg cggtgctgtc
     3901 gccggcgtac tcgccccact cggggttcca gccgttggcg tagatcgact tggcgttcgc
     3961 gccggcgagg ccgcggatga tcccgaactg gtggaccgcc gcctcgatca gcagcgggtc
     4021 cttgatccgg tggcggaagt cgccccagtc gccgtgcttc cggttgttga gcgcgagccg
     4081 gccgacgatg tagctcaccg ggccgtatgc gccggaccgc accagccgct cgagcgtctg
     4141 cttgtcccgg tcgaatcgat gactcatggt gatggccatc tttcggccgg cgcgggtgac
     4201 cttcgcgtgg atccgcgccg aggcggccat cgtgtccgcg accggcttct cgcacaggat
     4261 gtccaggccg tgggccagcg cgaggtcgat gatcgcctcg tgatgggccg gcggcacgac
     4321 cacgaccgcg aagtcggcct ccgcgccgcg ctccgagaag gcgcgagccg cgtccgtgta
     4381 gcaacggtcc tcggggatcc cgtagccctc gcgcgcgatc gcgaggttcg cctcgctcat
     4441 atcaaccgcg gcgacgggct cggcgagtcc cagccggatc acgtgcggca ggaacgtccg
     4501 cgaccacatg cggccccagc caccggtgcc gacgtggatg aaccgctgca cggtcggcct
     4561 cggtcctcgg tcaggccggg tcgagcggcg ggtg
//
LOCUS       DATBSW010000057         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_639432, whole genome shotgun sequence.
ACCESSION   DATBSW010000057 DATBSW010000000
VERSION     DATBSW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639432"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..649
                     /gene="mscL"
                     /locus_tag="VIF63_01450"
     CDS             257..649
                     /gene="mscL"
                     /locus_tag="VIF63_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY8238074.1"
                     /translation="MVSEFREFLLKTNALALAIGVIIGTALGTVVNSLVNDIIMPPIG
                     LVLGGVDFSALQITLKDAAGEVPAVAIRYGAFLNSIIAFVVIGFVIWRLSKMIIKEEV
                     AGPSKTCPFCKEANAVDATKCRACASTI"
     gene            797..1894
                     /gene="ugpC"
                     /locus_tag="VIF63_01455"
     CDS             797..1894
                     /gene="ugpC"
                     /locus_tag="VIF63_01455"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8238075.1"
                     /translation="MATVTFDHVWKRFGDFAAVKDLNLGIQDGEFMVLVGPSGCGKTT
                     SLRMIAGLEEVTEGDLKIGDRVVNDVAPKDRDIAMVFQSYALYPHMSIYDNMAFGLKL
                     RKVPKAQIDARVKDAARILNLENLQKKPRELSGGQRQRVALGRAIVREPAVFLMDEPL
                     SNLDAKLRVQTRAEIARLHQRLGTTTVYVTHDQVEAMTMGTRIAVMDKGELQQVGTPQ
                     DLYENPKKLFVAGFIGSPSMNFLDVTRDADGVKGDGVTLPIPTRYRGGASNATSLVAG
                     FRPEHLELGDIPNSATIRAKADVVEFLGDEELLHVTLPGHTGDIVAVVSSENRVKPGD
                     ILDLKLPLEKLHLFDKESGDSLAYAGAAAAA"
     gene            complement(1924..2727)
                     /locus_tag="VIF63_01460"
     CDS             complement(1924..2727)
                     /locus_tag="VIF63_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8238076.1"
                     /translation="MKIQEVGDRVFTLHFDFYDQQIGVVLGGSDVMVIDTRSTPVQAR
                     EIVAGIRELTRDPVSIVVDTHWHYDHTFGNSVFRPASIWGHVRAAERLRRDGLATIAE
                     IAAELPALAADVVDVQIDPPEHTFEDSATVAVGDRVVELSYHGRAHTDGDIAVVVPDA
                     NVLFGGDLIEEGSPPSFGDSFPIDWPGTVERLLPLATGAVVPGHGKVGDRAFLEAQIT
                     AFRGLAALARDVYTGNRTIDEAIAESPFGPRTPADAFERALRQLRGEQD"
BASE COUNT          464 a          941 c          966 g          445 t
ORIGIN      
        1 cagtcctcgt tgaatcggga tgacggtttg tcatgacggc atatcggcaa tcttgcagat
       61 tgcagaccgg atcgatctcc catcctcccg ggcatcgatc ggggcgggcc cgctgggcag
      121 ttgggcccgc ccatttacac ggggggcccc ggcggaaacg ccggggcttt cttctcgccc
      181 gattaacgga ctaggatggg cgcggtcgcc ccgcgcagtg atgcgcctcg gggcctgcga
      241 ttggaggagg cccaggatgg tcagcgaatt ccgcgagttc ctgctgaaga cgaatgcgct
      301 ggcgctggcg atcggcgtga tcatcggcac ggcgctcggc accgttgtca actcgctggt
      361 aaacgacatc atcatgccgc cgatcgggct cgtcctcggt ggggtcgact tctcggccct
      421 gcagatcacg ctgaaggatg ccgccggtga agtccccgcc gtcgcgatcc gctacggcgc
      481 gtttctcaac tcgatcatcg ccttcgtcgt gatcgggttc gtcatctggc ggttgtcgaa
      541 gatgatcatc aaggaagagg tcgcgggacc gtcgaagaca tgccccttct gcaaggaagc
      601 gaacgcggtc gacgcgacca agtgccgggc ctgcgccagc acgatctgaa atcgggctac
      661 gcgacggcgg ccatctggat ttacaatgcc cgcgtcccgg aatcggtgac acgtacgggg
      721 cccgtcgacg gcgcgcttca ggcgcgtcga gatgcaaggg cccgcgatgt catcaggatc
      781 gggcgggaga cgtgtcatgg ccaccgtgac gttcgaccac gtctggaagc gcttcggcga
      841 cttcgccgcc gtcaaggacc tcaacctcgg gatccaggac ggtgagttca tggtcctcgt
      901 gggcccgtcg ggctgcggca agacgaccag cctgcgcatg atcgccggcc tcgaggaggt
      961 caccgagggc gatctcaaga tcggcgaccg cgtcgtcaac gacgtcgcgc cgaaggatcg
     1021 cgacatcgcg atggtcttcc agagctacgc cctgtacccg cacatgtcga tctacgacaa
     1081 catggcgttc gggctcaagc tgcgcaaggt gcccaaggcc cagatcgacg cgcgggtcaa
     1141 ggacgccgcc cggatcctca acctcgagaa cctccagaag aagccgcgcg agctgtccgg
     1201 cggccagcgc cagcgcgttg cgctcggtcg ggcgatcgtc cgcgagccgg cggtattcct
     1261 catggacgag ccgctctcca acctcgacgc aaagctgcgc gtccagacgc gagccgagat
     1321 cgcgcgcctt caccagcgcc tcggcacgac cacggtctac gtcacccacg accaggtcga
     1381 ggccatgacg atgggcacgc ggatcgccgt catggacaag ggcgagctcc agcaggtcgg
     1441 cacgccgcag gacctgtacg agaacccgaa gaagctcttc gtggccggct tcatcggcag
     1501 cccgtcgatg aacttcctcg acgtgacccg cgatgcggac ggcgtcaagg gcgacggcgt
     1561 caccctgccg atcccgacgc gctatcgggg cggtgcgagc aacgccacct cgctcgtggc
     1621 cggcttccgg cccgagcacc tcgagctcgg cgacatcccg aactcggcga cgattcgggc
     1681 caaggccgat gtcgtggagt tcctcgggga tgaggagctg ctgcacgtga cgctcccggg
     1741 ccacacgggc gacatcgtcg cggtcgtgag ctccgagaac cgcgtgaagc cgggcgacat
     1801 tctcgacctc aagctgccgc tcgagaagct ccacctcttc gacaaggagt ccggcgactc
     1861 gctcgcgtac gcgggcgccg ccgcggccgc ttagctcgag gtgcctcggg gcactccgag
     1921 gcactagtcc tgctcgcctc gtagctggcg gagcgcacgc tcgaacgcgt cggccggagt
     1981 tcgcggaccg aacggtgatt cggcgatcgc ttcgtcgatc gtccggttgc ccgtgtagac
     2041 gtcgcgggcg agggcggcga gtccgcggaa cgccgtgatc tgcgcttcga ggaacgcccg
     2101 gtcgccgacc ttgccgtgac cgggcacgac cgcgccggtc gcgagcggga gcagccgctc
     2161 caccgtgccc ggccaatcga tcgggaagct gtcgccgaag ctcggtggcg agccctcctc
     2221 gatgaggtcg ccgccgaaga ggacgttcgc atcgggcacg acgaccgcga tgtcgccgtc
     2281 ggtgtgcgcg cggccgtggt agctcagctc gacgacgcgg tcgccgaccg cgaccgtcgc
     2341 cgagtcctcg aacgtgtgct ccggcgggtc gatctggacg tcgacgacgt cggcggcgag
     2401 cgccggcagc tccgccgcga tctccgcgat cgtggcaagg ccgtcgcggc gcagccgctc
     2461 ggccgcgcgc acgtggcccc agatcgaggc ggggcggaag acgctgttgc cgaaggtgtg
     2521 gtcgtagtgc cagtgcgtgt cgacgacgat cgagaccggg tcgcgggtca gctcacggat
     2581 cccggcgacg atctcgcgcg cctggacggg cgtcgaccgg gtgtcgatca ccatgacgtc
     2641 gctgccgccc agtacgaccc cgatctgctg gtcgtagaag tcgaaatgga gggtgaagac
     2701 gcggtcgccg acttcctgga tcttcatgac cgcagcgtac cggcgtcgcg cgctggcgta
     2761 ggctctcggc gatgagcgtg acggcccccg ccgaagcatc ggaagcgcga gtcgac
//
LOCUS       DATBSW010000058         7380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_357398, whole genome shotgun sequence.
ACCESSION   DATBSW010000058 DATBSW010000000
VERSION     DATBSW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357398"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VIF63_01465"
     CDS             complement(<1..523)
                     /locus_tag="VIF63_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8238077.1"
                     /translation="MDRALARRLEARFGGVLHGPARQRHDQTEIRIGAGDVQDVLRSL
                     HDEPDLAFVMLADLAGVDTGTEMQVVYHLWSATTDDWLRVIAEGLSRDDPRVPSVTFL
                     WQGAEWMERETYDMFGITFEGNRDLRRIYMPPDYTSFPLRKDFYLPDDAARSPGAGVR
                     HMERTKSPVDAAPG"
     gene            complement(528..1280)
                     /locus_tag="VIF63_01470"
     CDS             complement(528..1280)
                     /locus_tag="VIF63_01470"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEY8238078.1"
                     /translation="MTDKPALEPAEEGAVATMSSDQTGNQPSALIVPGSTAVAEPIVL
                     ANSLWSAANPAAYDGGRAPETTHLAEFGATMEGRRDDARWSGILEIIPTKADYVLDLL
                     RANSLWPLLSGLACCAIEMMSFATSKNDIDRFGAFPFRASPRQADVLIVAGTLTTKMA
                     GPLLRLWEQMPEPKWCVAMGDCTCSGGRYKRSYSTIEGIDRIMPVDVYVPGCPPRPEG
                     LIFGMMKLQQLIKDRRGHWPERAIGPTVPAGI"
     gene            complement(1277..1690)
                     /locus_tag="VIF63_01475"
     CDS             complement(1277..1690)
                     /locus_tag="VIF63_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8238079.1"
                     /translation="MTGIWYVIGFAVAAGGFAFLTFGAAWLVSHRNRGDVHKGLPYES
                     GIDTYGDTHGRFGLSFYIYALLFVAFDIEVVFIYLWAVIFQATVAAKRANQASLADPA
                     ALFASMVVFVGILLFGLAYAWRKGVLSWRGPGTDR"
     gene            complement(2054..3394)
                     /locus_tag="VIF63_01480"
     CDS             complement(2054..3394)
                     /locus_tag="VIF63_01480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8238080.1"
                     /translation="MKPADEHVSPAIEVGVRRIPELDPGAPLPPSDPLLVSRITAEIE
                     ASGPMTFARFMEIALYDPDAGYYVRDAAAADGAGVNTETPAGPGRTADFLTAPEGHPI
                     FGWVIARHLESVWDALDRPSQFVIREHGAGTGALAAGILDGLRRSGSELLAAVRYQAI
                     DLSPARLAALVDRLATLNGLDRYLLPRSDSTPAPGVILANELLDALPVHRVEGGSDGQ
                     LLERFVTVAGGTGPRGDNVQGGAHSQRAAAPQAPGSPTAESHFTTVLDAPSTPALAAR
                     LAAEGIALAPGQAAEICLAIDDWIAAATGPLERGELLLIDYGYPADQLYRPERGSTLR
                     AYHRHRVHADPLVAIGRQDLTAHVDVTAVERAAVAAGLEPIGRTTQAQFLADLGAGEL
                     LVALQSEPNATLESYLEARSALVRMLDPRATGAFAVLRFGRGIKRGELFGSSPG"
     gene            complement(3391..4560)
                     /locus_tag="VIF63_01485"
     CDS             complement(3391..4560)
                     /locus_tag="VIF63_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238081.1"
                     /translation="MPHAAVRILHLEPEEGAGELTRLLARARSIAAEDLARRFESAGA
                     DDVRLEACPRDALSFGARLRAVAGSLETGAGLVVLGSGSIALASDDDLAGLVAVAVSG
                     ERRALTNNRYSSDVVAVGDAAILAAAPDLPADNALPRWLTTRGVPVEELPDRDRLALD
                     LDSPLDLELVRRHPRCPPALADLARSMAHRLGRVAEAFDELAALARKPRMELLVAGRI
                     SASTLRSLEVDTACRIRALVEERGLRASAPFTADGGTDSGDEGSDGLDEGEPQAQRPP
                     ASVLGLVLDLEGPAEIGMVVDRLADGAIIDSRVLLAHRLGADESSWPSPEDRYASDLL
                     LADRVRDPWLQPLTLHAWSHKVPIALGAHSLVGPGLRLALGLDPPTGRLAQDAST"
     gene            4593..5732
                     /gene="folK"
                     /locus_tag="VIF63_01490"
     CDS             4593..5732
                     /gene="folK"
                     /locus_tag="VIF63_01490"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY8238082.1"
                     /translation="MRRVRAYIGLGANVGDARRTLTAAVAALAALPDADLRGVSRLYR
                     TKPVGVVDQPDFLNAVAALDVPAGPDPATGAMALLIALKHIERAFGRQVRGRWGPREL
                     DLDLLLFGDAHLAIDRPPEGMPQSAAIDPGAAARLLEVPHPSIAERLFVLAPLADLAP
                     GLVPPGWTQAIGTTQRKQVAAEGPHAVVPIGEWSPDEGTWIGPSGGPIDIRRAVPKDA
                     EAAARAHTASAEAAYRGIAPFEANGLARRTGVWREILADPSNPAFVAEDQGRIVGVLN
                     IGPWRGTTSGAVRILYVVPAWWGAGAGQALLDRAHAELAEDFDDAMLTVLAENARARR
                     FYERNGWQLQELLVEPHFGGHPTEVARYGRQLRRPPASKGQGRGA"
     gene            complement(5729..6436)
                     /locus_tag="VIF63_01495"
     CDS             complement(5729..6436)
                     /locus_tag="VIF63_01495"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEY8238083.1"
                     /translation="MAALPRILAMGGGSVMPNSTDGPLHRFMLDMTGKDRPRVCFIGT
                     ASGDDPSYRAGFYATFAQHSEATHLALFDRTVEDIVSFLLDQDVIYVGGGNTANMLAI
                     WRLHGVDKALKAAWEDGVVLAGPSAGANCWFQSSVTDSFGTNLAALKDGTGFLPGSFC
                     PHYDSESLRRPRYEELVGSGALPDGFAADDGCALVFNGRELGEAVASLPGQHAYRVER
                     KRGSSVEETQIRARLLR"
     gene            complement(6436..>7380)
                     /gene="glpX"
                     /locus_tag="VIF63_01500"
     CDS             complement(6436..>7380)
                     /gene="glpX"
                     /locus_tag="VIF63_01500"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY8238084.1"
                     /translation="TEEAAIASARAMGRGDRDGADRLATEAMRSTMDEIDFAGRIVIG
                     EGERDEAPMLYIGEEVGRRGARADLPEVDIAVDPLEGTNLVAHGQAGAITVLAASEAG
                     GLTHAPDTYLEKLCVGPGAAGKVDIRLPPAENVHLIAQALGRRPQDITVIILDRPRHE
                     DLIAEVRGTGARIKLITDGDLSAAISCAVQGTGVHAVMGIGGAPEGVITAAAMRCLGG
                     EIQARFKYRNDEERKRGDRMGHGDESRIYKTEDLASGEHLVFAATGVTPGDLLEGVRF
                     FGGGARTHSMVMAYQTKTVRFVDTVHMFDRDSPPRVRL"
BASE COUNT         1078 a         2587 c         2688 g         1027 t
ORIGIN      
        1 tgcccggagc cgcgtcgacc gggctcttcg tccgctccat gtggcggacg ccggcgcccg
       61 gcgaccgggc ggcatcatcg ggcaggtaga agtccttgcg cagcgggaag ctcgtgtagt
      121 cgggcggcat gtagatccgc cgcaggtcgc ggttgccctc gaacgtgatc ccgaacatgt
      181 cgtacgtctc gcgctccatc cactcggcgc cctgccacag gaaggtcacc gacggaaccc
      241 gcggatcgtc gcgcgacagg ccctcggcga tgacccggag ccagtcgtcc gtcgtggcgc
      301 tccagaggtg gtagacgacc tgcatctccg tccccgtgtc gacgcccgca aggtcggcca
      361 gcatgacgaa cgccagatcc ggctcgtcgt ggagggacct gaggacgtcc tggacgtcgc
      421 ccgccccgat cctgatctcg gtctggtcgt ggcgctgccg tgccggcccg tgcagcacac
      481 cgccgaatcg cgcctccaac cggcgcgcga gcgctcgatc catcgactca gatcccggcc
      541 gggaccgtcg ggccgatcgc gcgctccggc cagtggccgc ggcgatcctt gatgagctgc
      601 tgcagcttca tcatcccgaa gatcaggccc tcggggcgcg gcgggcagcc gggcacgtac
      661 acgtcgaccg gcatgatccg gtcgatgccc tcgatcgtgg agtagctgcg cttgtaacgg
      721 ccgcccgagc acgtgcaatc gcccatcgcg acgcaccatt tcggctccgg catctgctcc
      781 cacagccgca ggagcgggcc ggccatcttc gtggtgagcg tgccggcgac gatgagcaca
      841 tccgcctgcc gcgggctcgc ccggaagggg aaggcaccga accggtcgat gtcgttcttc
      901 gaggtggcga agctcatcat ctcgatcgcg cagcaagcca ggcccgagag cagcggccac
      961 aggctgttcg cccggagcag gtcgaggacg tagtccgcct tggtcgggat gatctcgagg
     1021 atgccggacc agcgggcgtc gtctcgccgg ccttccatcg tcgcgccgaa ctcggcgagg
     1081 tgggtcgtct cgggcgcgcg gccgccgtcg tacgccgcgg gattcgcggc ggaccagagc
     1141 gagttcgcca ggacaatggg ctcggcgacc gcggtcgaac cgggcacgat cagcgccgac
     1201 ggctggttgc cggtctggtc ggacgacatg gtcgcaacgg cgccctcttc ggcgggctcg
     1261 agcgcgggct tgtcggtcat cggtcggtgc ccgggccgcg ccagctgagc acgcccttgc
     1321 gccacgcata ggcgaggccg aacaggagga tgcccacgaa cacgaccatg ctggcgaaga
     1381 gcgccgccgg atccgcgagg gaggcctggt tcgctcgctt cgcggccacc gtggcctgga
     1441 agatcaccgc ccacaggtag atgaacacca cctcgatgtc gaacgccacg aacagcaggg
     1501 cgtagatgta gaacgagagc ccgaaccggc cgtgggtgtc gccgtacgtg tcgatgcccg
     1561 attcgtaggg caggcccttg tgcacgtcgc cccggttccg gtggctgacg agccaggccg
     1621 cgccgaacgt caggaacgca aaccccccgg cggcgacggc gaacccgatg acgtaccaga
     1681 tcccggtcaa ggccatcctc gctgagcgcc gggcttcgct tggcgctcga tcgttgggtt
     1741 gctggtgtga gaggcattcg cgaccaaccg tccaagaacg gccgtcgccc gaggccattc
     1801 taccgatccc gctctcaggt cgcctcgtgc tcggggggca cgcggcacgc ctgcgattcc
     1861 attggccgcc ggatgtccgc gtggaggacg agccggacga aatcccgctt cgccgtgcca
     1921 tcggcagccc gatctcgaca tggatggcct cgacacggac aaatcgaccc attcatgcgt
     1981 ggatctggcc aaattcacgg cgaatcgggc acctcgggcg agttccgagt ccgagttgtc
     2041 ctccacgccg acatcaaccg ggcgacgacc cgaatagttc gcctcgcttg atgccgcggc
     2101 cgaagcggag gaccgcgaag gcgccggtcg cgcgggggtc gagcatgcgg acgagcgcgg
     2161 agcgggcctc gaggtaggac tccagggtgg cgttcggttc cgactggagc gcgacgagca
     2221 gctcgcctgc gccgaggtcg gcgaggaact gcgcctgggt ggtgcggccg atcggttcga
     2281 ggccggcggc gacggctgcc cgctcgacgg cggtcacgtc cacgtgcgcg gtgagatcct
     2341 ggcgcccgat cgcgaccagc gggtcggcgt ggacgcggtg gcggtggtag gcccggagcg
     2401 tggaaccgcg ttcgggtcgg tagagctggt cagccgggta cccgtagtcg atgagcagca
     2461 gctcgccgcg ctcgagcggg ccggtggccg cggcgatcca gtcgtcgatc gcgaggcaga
     2521 tctcggcggc ctgcccgggc gcgagggcga tgccctcggc cgcgaggcgg gcggcgaggg
     2581 cgggcgtcga tggcgcgtcg agcaccgtgg tgaaatggga ctcggctgtt ggtgagccgg
     2641 gtgcttgggg agcggcggcg cgctggctat gcgcgccgcc ttgcacattg tcgccgcgcg
     2701 ggcccgtgcc gcccgcgacc gtgacgaacc gctcgaggag ctggccgtcg gagccgcctt
     2761 cgactcggtg gacaggcagg gcgtcgagca gctcgttggc gagaatcacg ccgggagctg
     2821 gggtcgaatc ggaccgcggg agcaggtatc ggtcgaggcc gttcagggtg gcgagtcggt
     2881 cgacgagggc ggcaaggcgc gcgggcgaga gatcgatggc ctggtagcga acggccgcga
     2941 gcagctcgga gccggatcgg cggaggccat cgaggatgcc cgcggcgagc gccccagtgc
     3001 cggcgccgtg ctcgcggatc acgaactgcg agggccgatc gagggcgtcc cagaccgatt
     3061 cgaggtggcg ggcgatcacc cagccgaaga tcgggtggcc ttccggggcc gtgaggaaat
     3121 cagcggtgcg acccggaccg gccggcgtct ccgtgttgac gccggcgcca tccgcagcgg
     3181 cggcgtcgcg cacgtagtag cccgcatccg ggtcgtagag cgcgatctcc atgaagcgag
     3241 cgaacgtcat cggcccggac gcctcgatct cggcggtgat ccgcgagacg agcaacggat
     3301 cgcttggcgg cagcggcgcg cccggatcga gctcgggaat gcgtcggacg ccgacctcga
     3361 tggcggggct cacgtgctcg tcggccggct tcatgttgag gcgtcctgcg ccagccgccc
     3421 cgtcggtggg tcaagcccaa gcgcgaggcg aagaccgggt ccgacgaggc tatgcgcccc
     3481 gagcgcgatc gggaccttgt gcgaccaggc gtgcagcgtc agcggctgga gccacggatc
     3541 acgcactcgg tcggcgagga gcagatcgga ggcgtagcgg tcctccgggc tgggccaact
     3601 cgattcgtcg gcgccgaggc ggtgggcgag gagcacgcgg ctgtcgatga tcgcgccgtc
     3661 cgcgagccga tcgacaacca tcccgatctc agccggacct tcgaggtcga gcacgagccc
     3721 gagcacggag gcaggcggac gctgcgcctg cggctcgccc tcgtcgaggc cgtcgcttcc
     3781 ttcatccccg ctgtcggtgc caccgtcggc ggtgaacggg gccgacgcgc gcagcccgcg
     3841 ctcctcgacc agcgcgcgaa ttcggcacgc cgtgtccacc tcgagcgagc gcagcgtcga
     3901 cgccgagatc cggccggcga ccagcagctc cattcgaggt tttcgtgcga gcgcggcgag
     3961 ctcgtcgaag gcttccgcga cacgccccag gcggtgcgcc atcgagcgcg caaggtccgc
     4021 gagggccggc gggcaccggg gatggcgccg gaccagctcg aggtcgagcg gggagtcgag
     4081 gtcgagggcg agacggtccc ggtccggcag ctcctcgacc ggcacgccgc gcgtagtcag
     4141 ccagcgcggc agggcgttgt ccgccggcag gtccggcgcg gcggcgagaa tcgccgcgtc
     4201 cccgaccgcc acgacatcgg acgagtagcg gttgttcgtc agcgcacgcc gctcgccact
     4261 gaccgcgacc gcgacgagtc ccgcgagatc gtcgtccgag gcgagcgcga tcgaccccga
     4321 acccaggacg acgagcccgg cgccggtctc gagcgatccg gcgacggcgc gcagccgcgc
     4381 cccgaacgac aaggcgtcgc gcggacacgc ttcgagtcga acgtcgtcag ccccggccga
     4441 ttcgaagcgt cgagcgaggt cttccgcggc gatcgaccgt gcccgagcga gcaggcgcgt
     4501 cagctcgccg gcaccctcct cgggctcgag gtgcaggatt cgaacggcgg catgcggcac
     4561 ggggccactc tagccgccac tgggaggtcg gcttgcgacg ggttcgggcg tacatcgggc
     4621 tcggggcgaa cgtcggcgat gcgcgccgga cgttgaccgc cgccgtggcg gcgctggctg
     4681 ccctgcccga cgccgacctc cgcggcgtat cccgcctcta tcgaaccaaa cccgtcggcg
     4741 tggtggacca gcccgacttc ctgaacgcgg tcgccgccct cgacgtccct gccggtccgg
     4801 atccggcaac gggcgccatg gcgctcctga tcgcgctgaa gcacatcgag cgcgcgttcg
     4861 ggcgccaggt ccgcggtcgc tggggcccgc gcgagcttga cctcgacctg ctcctcttcg
     4921 gcgacgcgca cctcgcgatc gatcgcccac ccgagggcat gccccaatcc gcggcgatcg
     4981 accccggggc cgcggcgcgc ctcctcgagg tgccgcaccc gtcgatcgcc gagcgcctct
     5041 tcgtcctcgc acccctcgca gatctcgcgc ccggcctcgt tccgccgggc tggacccagg
     5101 cgatcgggac aacccagcgc aagcaggttg ctgcggaggg accccacgcg gtcgtgccca
     5161 tcggcgagtg gagcccggat gagggcacgt ggatcggccc gagcggcggg ccgatcgaca
     5221 tccgccgcgc cgtgcccaag gacgcggaag ccgcggcgcg cgcccacacg gcgtcggcgg
     5281 aggccgcgta ccgaggcatc gcacccttcg aagcgaacgg gctcgcccgt cggacgggcg
     5341 tgtggcgcga gatcctcgcc gacccgtcga acccggcatt cgtcgccgag gaccagggcc
     5401 gcatcgtcgg cgtcctcaac atcggtccgt ggcgcggcac gaccagcggc gccgtgcgga
     5461 tcctgtacgt cgtgcccgcg tggtggggcg ccggcgcggg gcaggcgctg ctcgatcgtg
     5521 cgcacgcgga gctcgccgag gacttcgacg acgcgatgct caccgtcctc gcggaaaacg
     5581 cgcgggccag gcggttctac gaacggaatg gctggcagct ccaggagctc ctcgtcgagc
     5641 cccacttcgg cggccacccg accgaggtcg ctcggtacgg ccgacagctg cgtcggccgc
     5701 cggcctccaa gggccagggc agaggcgcct agcgcaggag gcgcgcccgg atctgcgtct
     5761 cctcgaccga gctgccccgc ttgcgctcga cgcgataggc atgctggccg ggcagcgacg
     5821 cgaccgcctc gccgagctca cgcccgttga acacgagggc gcagccgtcg tccgctgcga
     5881 acccgtcggg cagcgcgccg gagcccacga gctcctcgta ccgaggccgg cggagtgact
     5941 cgctgtcgta gtgggggcag aagctcccgg gcaggaatcc cgtgccgtcc ttgagcgcag
     6001 ccaggttcgt gccgaaggaa tccgtgacgg agctctggaa ccagcagttc gcacccgccg
     6061 acggcccggc aaggacgacg ccgtcctccc aggcagcctt gagcgccttg tcgacgccgt
     6121 gcaggcgcca gatcgcgagc atgttcgcgg tgttcccgcc gccgacgtag atcacgtcct
     6181 ggtcgagcag gaaggacacg atgtcctcga ccgtccgatc gaacagtgcg aggtgggtcg
     6241 cctcggagtg ctgggcgaag gtcgcataga acccagcccg atagctcggg tcgtcgccgg
     6301 acgcggtgcc gatgaaacag acccgcggcc ggtccttgcc ggtcatgtcg agcatgaatc
     6361 gatggagcgg gccgtcggtc gaattgggca tgaccgaacc gccacccatc gccaggatcc
     6421 gcggtagcgc cgccatcaga gccggacgcg cggcggcgaa tctcgatcga acatgtggac
     6481 cgtgtcgacg aagcggacgg tcttcgtctg gtaggccatc accatcgagt gggtccgcgc
     6541 gccgccgccg aagaagcgca cgccctcgag caggtcacct ggcgtgacgc cggtcgcggc
     6601 gaagacgagg tgctcgcccg aggcgaggtc ttcggtcttg tagatgcggc tctcgtcgcc
     6661 atggcccatc cggtcgccgc gcttgcgctc ctcgtcgttg cggtacttga accgcgcctg
     6721 gatctcgccg ccgaggcagc gcatcgccgc ggcggtgatc acgccctcgg gcgcgccgcc
     6781 gatgcccatg accgcgtgga cgccggtgcc ctggaccgcg cacgagatcg cggccgagag
     6841 gtcgccgtcg gtgatcagct tgatccgcgc cccggtgccg cgcacctcgg cgatgaggtc
     6901 ctcgtggcgc ggtcggtcga ggatgatcac ggtgatgtcc tgcgggcggc gcccgagggc
     6961 ctgggcaatg aggtggacgt tctcggccgg cggaaggcga atgtcgacct tgcccgcggc
     7021 gcccggcccg acgcacagct tctcgaggta cgtgtccggg gcgtgggtga ggccgccggc
     7081 ctccgacgcc gcgaggaccg tgatcgcacc ggcctggccg tgcgccacca ggttggtgcc
     7141 ttcgagcggg tcgaccgcga tgtcgacctc cggcaggtcg gcgcgcgcgc cccgccggcc
     7201 gacctcctcg ccgatgtaca gcatcggggc ctcgtcgcgc tcgccctcgc cgatgacgat
     7261 ccgcccggcg aagtcgatct cgtccatggt cgagcgcatg gcctcggtcg ccagccggtc
     7321 cgcgccgtcc cggtcgccgc ggcccatcgc acgcgcggag gcgatcgccg cctcctccgt
//
LOCUS       DATBSW010000059        19963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_745174, whole genome shotgun sequence.
ACCESSION   DATBSW010000059 DATBSW010000000
VERSION     DATBSW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19963
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745174"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1197)
                     /locus_tag="VIF63_01505"
     CDS             complement(97..1197)
                     /locus_tag="VIF63_01505"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8238085.1"
                     /translation="MTNSTPLTVAVVGATGVVGRTMVQVLGERSFPIGELRLLASGRS
                     AGRTVEVDGRTVEIREAVGDEFEGVDIALFSAGADISRDLAPQAAERGATVIDNSSAW
                     RMDPKVPLVVSQVNPDDLEAHEGIIANPNCSTMQLAPVLMALRDSVGIERVVVDTYQS
                     VSGTGADAIAELEGQIRAHVAGEPKQAGVYPHPIAFNALPEIDTFLDNGYTKEEWKIV
                     TESRKILHLPELRISCTAVRIPVFVSHSEAVHVETVDPLTPERARRLFEAVAGVVVQD
                     DPQTHAYPLATEAAGRDEIFVGRVRQDDSIPRGRGLAFWVVSDNLRKGAATNAVEIAE
                     ALVERDWVQARSRRRPHAATEPIGTTTVETRA"
     gene            complement(1375..3963)
                     /gene="pnp"
                     /locus_tag="VIF63_01510"
     CDS             complement(1375..3963)
                     /gene="pnp"
                     /locus_tag="VIF63_01510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8238086.1"
                     /translation="MPQVFETQLGGRTLTIETGRLARLAGGAVTVRYGDTMVLGTANR
                     SEPRPGLDFFPLTVDFEERMYAAGKIPGGFIKRESRPSENAILAARQTDRPMRPLFPE
                     GYKDDIQLVITVLSTDQENNPDVLGTVAASTALTMSEIPFNGPVGAVRIGRIGGEFVV
                     NPTYTELQESDLDLIVAGTRDAIMMVEAGANLLPEDVMAEAILFGHRSLQPLVDIQDE
                     IRKALGKPKRVPYIEPSTGSVLEFATATDAKRELVVIDVETTGTDPKMSDLLEVAAVK
                     IKGTKVVDRWSTLVNPGRSIIGNQVHGITDKDVKGAPTPKAAAEQLLKFVGDATVVGH
                     NVGFDLGFIEEAKGDGFRFAPGSYLDTLVIAREGYPGESSYKLPDLARFFGIELGQSH
                     RALPDAEATASLLLWFANDLPGRITKLKGAIADAVRANRTGGDTNALLEAARRDARVS
                     KGLFSLIQKKTVRRVVIDEGIRIDGRGLTDIRPITVEVGLVPRAHGSGLFTRGETQAL
                     TVATLGASSDVQRLDNISPETEKRYIHHYNFPPYSTGENKPMRGPSRRDIGHGHLAER
                     ALVPVLPSHEEFPYVIRLVSECVTSNGSTSMASTCGSTLALMDAGVPIKAPVAGAAMG
                     LITEPDGTFVVLTDILGKEDAIGDMDFKVTGTRDGVTALQMDIKVQGISEAIIREGLK
                     QALAARLTILDKMAEVLPEAREVMSDFAPRITTIKINPEKIREIIGKGGSMIRKIQEE
                     TQTEINVEDDGSVEIAAVSGENARKAIAWIESLTKEVEVGALYLGKVTRLMGFGAFVE
                     ILPGKEGLVRIGELADYHVPTVEDVVSVGDEVMVVVIEVDRQGRINLSRKAAMQRHLA
                     KEPVEA"
     gene            complement(4073..4342)
                     /gene="rpsO"
                     /locus_tag="VIF63_01515"
     CDS             complement(4073..4342)
                     /gene="rpsO"
                     /locus_tag="VIF63_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8238087.1"
                     /translation="MPLAPEGKTETIIEYAVSDGDTGSPEVQIALLTERIKGLTDHLR
                     SFPKDNHSRRGLLKLVGQRRRLLAYLLKKDNARYRAVIGRLGLRR"
     gene            complement(4442..5257)
                     /locus_tag="VIF63_01520"
     CDS             complement(4442..5257)
                     /locus_tag="VIF63_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HEY8238088.1"
                     /translation="MPAQPSPRMAVVEGLEGLDASLGRLFLVIGVFDGLHRGHAYLLD
                     ELRRAAQAHDARPAVLTFDHHPDEILTGAAPPLLLDPAERLERLEAAGVAVTVVQHFD
                     RALRETPYDAFVRRIADRVELRGFLMTPDSAFGYERAGTPETVAALGRELGYEVEVVP
                     QFTLEGEPVSSSKVRAAIAAGDLGAAERLLGRPYAVVGRGSRISRDPVLTFETPVALP
                     PPGWYTVQIDDLDEGTTHSPDGVRVLDAATVRLTGIWLGGPRWRVQFEPAATG"
     gene            complement(5257..6192)
                     /gene="truB"
                     /locus_tag="VIF63_01525"
     CDS             complement(5257..6192)
                     /gene="truB"
                     /locus_tag="VIF63_01525"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8238089.1"
                     /translation="MARQPDAPGMHGVLIVAKPAGPTSHDVVALVRRLASTRRIGHGG
                     TLDPFASGVLPLFLGGATRLVEYHLSDDKAYRATICFGATSTTDDLDGELTPIEAPAP
                     SRDEVEGALRGFLGGQLQQPPVYSAVQVGGRRAYAMARAGETVDLPSRSVEIKGLDLL
                     EWDGTDPDRPIAIVDVRCSAGTYVRALARDLGARLGCGAYLGALVRLASGPFTLEGAR
                     SLDEIRSAAAESGPDGVKALLLPSDTGLDALPSIALTDAEIADALQGRFVRPAAGLRD
                     APEGEPLRLLDGAGLIVGMGRRDGTRVAPTKMLPR"
     gene            complement(6201..7235)
                     /locus_tag="VIF63_01530"
     CDS             complement(6201..7235)
                     /locus_tag="VIF63_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY8238090.1"
                     /translation="MSIDLRPWLDAVPDAAAAAIAGARRALVATHENPDADTLGAALG
                     IAAIVEHHGGRATLVSTDPVPPLYDFLEGMDRFVQDPAADGEYDLLVVCDCGSLDRLG
                     AVRERHPELFERLPRVLVDHHASNTDGRAGDWIDPAAAATCEMVALLAAKLDVPLDAA
                     NGLMAASLMAGIVMDTSTFAHPNATPRTLAVSAALVAAGAPLAEISRRLYRSKPDAQL
                     RLFGRVLDRLVVTDGGRVIHSSLDDADLAATGTLASHSEGLIDLLAQSETAEVAILFK
                     EAGPASTRLSVRTKPGGVDATVLTGAFGGGGHARAAGATVDAPLAEARRRVLAEAERL
                     VAAVSRSSAS"
     gene            complement(7232..7711)
                     /gene="rbfA"
                     /locus_tag="VIF63_01535"
     CDS             complement(7232..7711)
                     /gene="rbfA"
                     /locus_tag="VIF63_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8238091.1"
                     /translation="MTARTEKIDQLLRQEIGAILTRDVQDPRIGFVTVTDVETAPDLS
                     VARVWVSVIGQPAEREVTRRALEHAMPFVRRELGARVRLRRIPELQLRLDDTVQRGTR
                     VLQLLAELEAGGEVADAPPPEESLPTPVGQVPPMPEARAPRRRRDPRRAPRDRGARR"
     gene            complement(7745..9649)
                     /gene="infB"
                     /locus_tag="VIF63_01540"
     CDS             complement(7745..9649)
                     /gene="infB"
                     /locus_tag="VIF63_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8238092.1"
                     /translation="MARSRSGTRGRRGPRKPPRRDGAFANTAVQVLDPKDRGAVELPA
                     TITVKELADVLGVNPADVIRELIKSGIFATINQLIDRDTASLVASELGYDVAEPQAPG
                     VPVAGEDGVDAPQAATKEELFTEDDTADLVDRSPIVTVMGHVDHGKTSLLDAIRSTTV
                     AAGERGGITQHIGASEVHRGDRRVVFLDTPGHEAFTAMRARGARVTDVAVVVVAADDG
                     VMPQTLEAISHAKAAKVPIIIALNKIDKPDANPDRVKTELTEAGIVIEEYGGDVPLVP
                     VSAKSRVGLEDLVDMVLLVADLQELKANPKRTAIGTIVEARMDKARGAVGTVLVQTGT
                     LNVGDIIVVGETFGRVRALEDEHGKRIKKAGPATPAVILGLSQVPEAGDILRVVADEK
                     AARAAVEARTAEIAAKGGEGSGRATLEDLYRQIQAGQAKELRIVLKADVSGSLGAITH
                     ALGQLKTDEVKLNVLYEGAGEISDNDILLASASNAIVVGFNTAISDTARRAAESEKVD
                     VRMYDIIYQLTDDIEKALSGLLEPEAVEVIEGRAEVRKLIKVGRQVIAGSYVTDGRIV
                     RSGSARLWRGGKVIVTDKIDSLRRFKDDVREVQTGFECGIGLVSTTDILEGDVIECFS
                     TQMVSRSALS"
     gene            complement(9938..11380)
                     /gene="nusA"
                     /locus_tag="VIF63_01545"
     CDS             complement(9938..11380)
                     /gene="nusA"
                     /locus_tag="VIF63_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8238093.1"
                     /translation="MSRDFVGALLQLNAEKQVSREQLIRTVEDAIAAAYRRVANEEDI
                     HVQIDADSGRIRVYRARIASVDPIEEEQQLTHFTLDEAKRHKPDAIPGELVEIEELDG
                     DTFGRIGAQTAKQVVLQRLREAERDAVFDQFADREGELVTGQVNRVEPRAMILDMGKG
                     VEGILATTEQSPLEHYRIGQNVKSYVLEVRRTPRGPALFVSRTHKGFLKRLFELEVPE
                     IHNGTVEIRAIAREAGSRSKVAVASRQEGLDPVGATVGQRGARVQAVVAELGGEKIDV
                     IPWNDDPSAFVANALSPAQVISVEIDTEHRIANVTVPERMLSLAIGREGQNARLAARL
                     TGWRIDIRSDVSVAEAKAAAEKAPEAEAAPAAEPVVADAAEKPAKAAAKAAKANKAAA
                     TVAEAPAKAAKAPKTTKAAKAETPAAAATDAVIEVKAPKKPRATKAKVATATADAAPE
                     PDATPVESADAEAPKPKARKTKVAAAEVTS"
     gene            complement(11897..13141)
                     /gene="ispG"
                     /locus_tag="VIF63_01550"
                     /gene_synonym="gcpE"
     CDS             complement(11897..13141)
                     /gene="ispG"
                     /locus_tag="VIF63_01550"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY8238094.1"
                     /translation="MSAPVRRQTVTVDVGGVMVGSDHPVVVQSMTNTDTADADATAIQ
                     VARLAHAGSELVRVTVNNDAAAAAVPDMLRKLRDLGVGVPVIGDFHYNGHQLLVEYPE
                     MARGLAKYRINPGNVGAKRHDENFQTIVRVAVENDVPVRIGVNWGSLDQALLTLLMDE
                     NAKLAEPRESRDVIIEAMIESAMRSAELAEETGLGHDRIILSAKVSNVRDVVDVYRIL
                     ARRSDYPLHVGLTEAGLGMKGVVASTAALAVLLEEGIGDTIRVSLTPAPGGEREEEVR
                     VAQQVLQSMGLRSFLPQVSACPGCGRTTSTFFQEMAERIQGYLRDQMPGWQARYPGVE
                     SMTVAVMGCVVNGPGESKHADIGISLPGTFEEPVAPVFIDGKLDRTLRGEGLVDEFIG
                     ILEAYVDQRYGARSGAGVVAPA"
     gene            complement(13155..14240)
                     /locus_tag="VIF63_01555"
     CDS             complement(13155..14240)
                     /locus_tag="VIF63_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY8238095.1"
                     /translation="MDGLLYGPLAVLLFIVVLGGIVLVHELGHFITARRLGVRVLEFG
                     IGFPPRAKVLRNKGETLWTLNWLPIGGFVRMDGEDGDEVLDPRSFGAKSLKVRLAILV
                     AGVVMNIVLAFAIFFLIALLATPVLGARIVSVETGSPAAAAGLQPGDEIYHVAGQEFE
                     LFSGDSATQALRRHGGETITIGIRHPDGSEVDVSVALRPKSELSPTRGALGVSTEQTN
                     AYTSHDLGASVGIASSEVARWGGLIFRGLGDLVNGFIKDPTAEPPAAGPIGIAVSLAD
                     ILLNAGPIWLLYVAGILSVNLAVVNILPFPPLDGGRMAVLVVKRVFGARVSLRAERLT
                     YAVGFLFLMAFVLWISGFDIARLGSAP"
     gene            complement(14240..15481)
                     /gene="dxr"
                     /locus_tag="VIF63_01560"
     CDS             complement(14240..15481)
                     /gene="dxr"
                     /locus_tag="VIF63_01560"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8238096.1"
                     /translation="MSEPRDNRPRGIALLGSTGSIGRQALDVLAGDPAFRVVALAAGR
                     NQALLAEQVERFRPVAVALAEDAPDHPAFEHGLPNGTTVMRGDDALIELAIRDDVDLV
                     VVATGGVVSLRPVLAALEAGKIVATANKETLVAGGHLVMPLARAHAARRAAIDPADPL
                     ASPLGWLRPIDSEHSAIWQSLMGEDLARVERLILTASGGPFLDLPAADFAAITPASAL
                     RHPTWTMGAKITIDSATLANKGLEVIEARWLYDVAYEAIDVVIHPQSVVHSAVQFVDG
                     SLKAQLGTPDMRLPIQYALTWPDRRLSPAAAPDLIAIARLDFRAPDETRFPALRIARE
                     AGALGPRATAALIAADDVAVARFLDGTLDFPGIPRLLEDAVARFGTAAGADPNVEELV
                     ALDLEVRRSLAGAGAGTRVTA"
     gene            complement(15489..16319)
                     /locus_tag="VIF63_01565"
     CDS             complement(15489..16319)
                     /locus_tag="VIF63_01565"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8238097.1"
                     /translation="MLRDRARSAAILVPPLLVAMWLGGWAIVLVVAAVVVVAGYEAFK
                     LLTAAGFHSMPLFGIVLAVVVALGDGVKQVPGGSGLLLAGLGIILIGTAALTRLDPRE
                     GLAIFATTTFGALYVGLLGFIARLASTDSAVDPRSILGWLGPERAWIVVLVLVVWAFD
                     TLAYFVGKRFGRRPFMAHISPSKTVEGVIGGMAAAAVVGAVVVTLLGRPWLAGLVLGV
                     AIAAAAQAGDLAESMLKRAAGAKESGRLIPGHGGLLDRVDSFLFAAPVAYFYVVSILH
                     "
     gene            complement(16324..17124)
                     /gene="uppS"
                     /locus_tag="VIF63_01570"
     CDS             complement(16324..17124)
                     /gene="uppS"
                     /locus_tag="VIF63_01570"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY8238098.1"
                     /translation="MARAPLIRPTDAPPRAHEVEAAGLPIERPEVVAGKRGDRAEAPT
                     HVAIIMDGNRRWARARSVSEIEGHAAGVEAIRGLLRHAVRVGIPVLTLYAFSRENWAR
                     TDEEVVGLFGLLESAIRSETAELQAQGVRVRLLGRLEELPTATRTSIESALAATAGGD
                     RLVLNIAFNYAGRTELVDAVRDIVRAGLPPEAIDEATVSASLYTAGLPDPDLIIRTGG
                     EERLSNFLIWQSAYAELMTSDTLWPDFGPADLDLALAEYASRTRRFGR"
     gene            complement(17124..17681)
                     /gene="frr"
                     /locus_tag="VIF63_01575"
     CDS             complement(17124..17681)
                     /gene="frr"
                     /locus_tag="VIF63_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8238099.1"
                     /translation="MSNEIITTADHKMARAVEVMDRDLNSIRTGRASTGLVERIHIDY
                     YGTQTPLNQLAGISVPEPHQIVIQPWDRSALGAIERAILKSDIGLTPNVDGTVVRLNI
                     PLLTEDRRHDLVKQVHKRMEEARVEIRNLRRDAADHLKKLQKEGEVGVDEEHKLLESL
                     QKTTDRHVAEVDRVGAAKEQEVLEV"
     gene            complement(17678..18424)
                     /gene="pyrH"
                     /locus_tag="VIF63_01580"
     CDS             complement(17678..18424)
                     /gene="pyrH"
                     /locus_tag="VIF63_01580"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8238100.1"
                     /translation="MSDASGAPRYHRILLKLSGEALLGSRQYGVDPEVCAFIARQVAA
                     VRATGVEVGIVVGGGNIFRGLSAAAQGMDRATGDYIGMLATVMNGLALQDAIERTETP
                     TRVMSAIAMNEIAEPYIRRRAVRHLEKGRVVIFVAGTGNPYFTTDTAAALRAVEIGAE
                     VLMKATKVDGVYDADPMTVPGAHRYERLEYADLLRDQLKVLDGAAVSLCMENDLPIVV
                     FDLNGTDNIARVVAGETIGTLIAGPVGVRA"
     gene            complement(18421..19026)
                     /gene="tsf"
                     /locus_tag="VIF63_01585"
     CDS             complement(18421..19026)
                     /gene="tsf"
                     /locus_tag="VIF63_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8238101.1"
                     /translation="MANITAEAVRNLRERTGAGMMDCKRALDETDGDVDKAIAILRER
                     GLAAAAKKAGREAREGLVSSYIHTGGRVGVLIEVNCETDFVARTEQFQALVRNLAVQV
                     AATYPRFVDITDIPADVLAAKKTELLDATDVAAKPAEVREKIVDGRLKKWYAEPGGVL
                     LEQPYRDTEGTVRALVTEAVATIGENIQVRRFERYALGEDK"
     gene            complement(19121..>19963)
                     /gene="rpsB"
                     /locus_tag="VIF63_01590"
     CDS             complement(19121..>19963)
                     /gene="rpsB"
                     /locus_tag="VIF63_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8238102.1"
                     /translation="GIHIIDLAQTAKRLETALDFVRETVARGEVVLFVGTKKQAQEPL
                     AAEAIRAGMPFINKRWLGGMLTNFVTIKKRIGLMDQLEARQQAGDLDRLPKKEAAKLL
                     EELARLQLILGGIRKMKRLPGAIFIVDPHRERIAVTEANRLEIPVVGTGDTNVDPDEL
                     DYIIPANDDAIRAIRLLCSLVADAAIEGAQDRASRAIEPEVEIVDEPTYEDTEAATDE
                     LVAALAGGAALSFAPDDDDDLLPGAPVIPAVPEDAATPEEIAAELAREAAEAATREGD
                     PTPA"
BASE COUNT         2819 a         6759 c         7251 g         3134 t
ORIGIN      
        1 ccgcggcaat cgcctcgagg gcggcgcgtc gtgcttcggt cgacacgagg cttccgctcg
       61 acggtgcgcc ctcgctcgca gcgagccccg ccgcgatcac gcgcgcgtct cgaccgtggt
      121 cgttccgatg ggctccgtcg cagcgtgcgg gcggcgtcga gagcgcgcct ggacccagtc
      181 ccgctccacc agcgcctccg cgatctcgac ggcgttcgtg gccgcgccct tgcgcaggtt
      241 gtcggacacg acccagaagg ccagaccgcg tccgcgcgga atggagtcgt cctgccggac
      301 acgcccgacg aagatctcgt cccgaccggc cgcctcggtc gcgagcgggt acgcgtgcgt
      361 ctgtgggtcg tcctggacca cgacgcccgc cacggcctcg aacaggcgcc gtgcgcgctc
      421 tggcgtcagc gggtcgaccg tctcgacgtg gaccgcctcg gagtggctca cgaacaccgg
      481 gatgcgaacg gccgtgcacg agatgcgcag ctcgggcagg tgcaggatct tgcggctctc
      541 ggtgacgatc ttccactctt ccttcgtgta gccgttgtcg aggaaggtgt cgatctccgg
      601 tagcgcgttg aacgcgatcg ggtgcgggta cacgccggcc tgcttgggct cgccggccac
      661 gtgcgcgcga atctggcctt cgagctcggc aatcgcatcg gcgccggtcc cggagacgga
      721 ctggtaggtg tcgacaacga cgcgttcgat cccgacggaa tcgcgcaggg ccatgagcac
      781 cggcgccagc tgcatcgtcg agcagttggg gttggcgatg atgccctcgt gcgcctcgag
      841 gtcgtcgggg ttcacctggc tcaccacgag cggcaccttc gggtccatcc gccacgcgga
      901 cgagttgtcg atcacggtcg cgccgcgctc cgccgcctgc ggcgcgagat cgcgcgagat
      961 gtctgcgccg gcgctgaaca gggcgatgtc gacgccctcg aactcgtcgc cgacggcttc
     1021 ccggatctcg accgtccgac catcgacctc gaccgtgcgc ccggcggatc gacccgatgc
     1081 caggagccgc agctcgccga tcgggaagct gcgttcgccc aggacctgga ccatcgtccg
     1141 accgacgacg ccggtggcgc cgacgacggc aacggtaagc ggggtcgagt tggtcatccg
     1201 gggagggctc cgcgcatggc tccgcagggt gaaatggtca cgcgggcacg atcgggagta
     1261 tagccgaggc ccctccggac ctaccgtcgc ccggtcgcca ggcgcgcggc atttcccggg
     1321 aatcgaaaga ccccggcggg gccggggtct tcgttggctg tggcgccgcg agggctacgc
     1381 ctcgacgggc tccttcgcga ggtggcgctg catggccgcc ttgcgcgaga ggttgatgcg
     1441 gccctggcga tcgacctcga tcacgacgac catcacctcg tcgccgacgg acacgacgtc
     1501 ctcgacggtc ggcacgtggt agtcggcgag ctcgccgatg cggacgaggc cttccttgcc
     1561 cggcaggatc tcgacgaatg cgccgaagcc catcagtcgg gtgaccttgc cgaggtacag
     1621 cgccccgacc tcgacttcct tggtcaggct ctcgatccag gcgatggcct tgcgcgcgtt
     1681 ctcgccgctc accgcggcga tctcgacgga gccgtcgtcc tcgacgttga tctcggtctg
     1741 cgtctcctcc tggatcttgc ggatcatgga tccacccttg ccgatgatct cgcggatctt
     1801 ctcggggttg atcttgatcg tggtgatccg gggggcgaag tcgctcatga cctcgcgggc
     1861 ctcgggcagg acctcggcca tcttgtcgag gatcgtcagg cgggcggcga gcgcctgctt
     1921 gagcccctcg cggatgatcg cctcgctgat gccctggacc ttgatgtcca tctggagcgc
     1981 cgtcacgccg tcgcgggtgc cggtgacctt gaagtccatg tcaccgatcg cgtcttcctt
     2041 gccgaggatg tcggtcagga cgacgaaggt gccatcgggc tccgtgatca ggcccatcgc
     2101 cgcgccggca accggcgcct tgatcggcac gcccgcgtcc atcagggcga gggtcgagcc
     2161 gcaggtggag gccatcgagg tcgagccgtt ggaggtcacg cactccgaca cgagtcggat
     2221 gacgtacggg aactcctcgt ggctcggcag gacgggcacg agggcccgct cggcgaggtg
     2281 gccgtgcccg atgtcgcgcc ggctggggcc gcgcatcggc ttgttctcgc cggtgctgta
     2341 cggcgggaag ttgtagtggt ggatgtagcg cttctctgtc tcggggctga tgttgtcgag
     2401 ccgctggacg tccgaggacg caccgagcgt ggcaacggtc agcgcctggg tctcaccgcg
     2461 ggtgaacagg cctgagccgt gggctcgtgg caccaggccg acctcgaccg tgatcggtcg
     2521 gatgtcggtg aggccgcggc cgtcgatccg gatgccctcg tcgatgacga cgcggcggac
     2581 cgtcttcttc tggatgaggc tgaacaggcc cttgctgacg cgggcgtcgc gtcgagcggc
     2641 ctcgagcagc gcgttcgtgt cgccgcccgt gcggttggcc cgcacggcgt cggcgatcgc
     2701 gcccttgagc ttcgtgatcc gacccggcag gtcgttcgcg aaccacagca ggagacttgc
     2761 ggttgcctcg gcgtccggga gcgcccgatg gctctggcca agctcgatgc cgaagaagcg
     2821 agccaggtcg gggagcttgt aggaactctc gccggggtaa ccctcacgag cgatcacgag
     2881 ggtgtcgagg tacgagcccg gagcgaaccg gaacccgtcg cccttggcct cctcgatgaa
     2941 gccgaggtcg aagcccacgt tgtggcccac gaccgtggcg tcgccgacga acttgagcag
     3001 ctgctctgcc gccgccttgg gggtcggggc gcccttgacg tccttgtcgg tgatgccgtg
     3061 gacctggttg ccgatgatcg agcggccggg gttgacgagc gtcgaccagc ggtcgacgac
     3121 cttcgtgccc ttgatcttga ccgcggcgac ttcaagcagg tcggacatct tggggtccgt
     3181 cccggtcgtc tcgacgtcga tcacgaccag ctcgcgcttg gcgtcggtcg cggtggcgaa
     3241 ctcgagcacc gagccggtgg agggctcgat gtacgggacg cgcttgggct tgccaagcgc
     3301 cttgcggatc tcgtcctgga tgtcgacgag gggctggagc gagcgatgcc cgaacaggat
     3361 ggcctcggcc atcacgtcct cggggagcag gttcgcgccg gcctcgacca tcatgatcgc
     3421 gtcgcgggtg ccggcgacga tcaggtcgag gtccgactcc tggagctccg tgtacgtcgg
     3481 gttgacgacg aactcgccgc cgatccggcc gatgcgcacg gcgccgacag gcccgttgaa
     3541 cgggatctcg ctcatcgtca gcgccgtgga ggcggcgacg gtgccgagga cgtccgggtt
     3601 gttctcctga tcggtcgaga ggaccgtgat cacgagctgg atgtcgtcct tgtagccctc
     3661 gggaaagagg ggccgcatcg ggcggtcggt ctggcgggct gccaggatcg cgttctccga
     3721 cgggcgcgac tcgcgcttga tgaagccgcc cgggatcttg cccgcggcgt acatccgctc
     3781 ctcgaagtcg accgtgagcg ggaagaagtc cagccccgga cggggctcgg accggtttgc
     3841 ggtgccgagc accatggtgt cgccgtagcg gacggtgacg gcgccgccgg cgaggcgtgc
     3901 aagccggccg gtttcgatgg tcagcgttcg accaccgagc tgggtctcga agacctgagg
     3961 cactggttgt gaatcctcct gccgtggagg agtgactgac ctgcggcggg tcgacccgac
     4021 gcgctatccc tgcgctcccc tgcccgttcc gggccagggc ggcgatctaa ggctagcggc
     4081 ggagtccgag ccgtccgatg acggcgcggt agcgcgcgtt gtccttcttc agcagatacg
     4141 cgagaaggcg acggcgctgg ccgacgagct tgaggaggcc gcgacgcgaa tgattgtcct
     4201 tcgggaagct gcggagatgg tccgtcaggc ccttgatccg ctccgtcagg agcgcgatct
     4261 ggacctcggg cgagccggtg tcgccgtcac tgacggcgta ctcgatgatg gtctccgttt
     4321 tcccttcggg cgcgagcggc actcggtcct ccgacacgaa caacactatt gggttctctt
     4381 ccacatctat gaccggcgga tggtagcagc caatccccga tcacgcacct ttggcccctg
     4441 gtcagccggt ggcggccggc tcgaattgga cgcgccagcg cgggccgccg agccagatgc
     4501 ccgtgaggcg gacggtcgcc gcatccagga cgcgcacccc gtcggggcta tgggtcgtgc
     4561 cctcgtcgag gtcgtcgatc tggacggtgt accagcccgg cggtggcagg gcgacgggtg
     4621 tctcgaacgt gagcaccggg tcacggctga tgcgcgatcc gcggccgacg acggcatagg
     4681 gccggccgag caatcgctct gcggcgccaa ggtcgccggc ggcaatggcg gcacggacct
     4741 ttgagctgct cacgggctcg ccctcgaggg tgaactgagg cacgacctcc acctcgtagc
     4801 cgagctcgcg tccgagcgcc gcgacggtct cgggcgtccc ggcgcgttcg tagccgaagg
     4861 ccgagtccgg ggtcatcagg aagccacgga gctcgactcg atccgcgatc cggcgcacga
     4921 aggcgtcgta gggggtctcg cggagggcgc gatcgaagtg ctggacgacg gtgacggcga
     4981 ctccggccgc ctcgagccgc tccaaccgct cggcggggtc cagcaggagc ggcggcgccg
     5041 cgccggtcag gatctcgtcg ggatggtgat cgaaggtgag caccgccggg cgggcgtcgt
     5101 gggcctgggc ggcgcggcgc agctcgtcga ggaggtaggc gtgaccccgg tgcaggccgt
     5161 cgaacacgcc gatgacgagg aacaggcgcc cgagggaggc atcaagcccc tcgagccctt
     5221 cgaccaccgc catccgtggc gacggctgcg ccggcatcag cgcggcagca tcttcgtggg
     5281 cgcgacgcgg gtgccatccc gccggcccat gccgacgatc agcccggcgc cgtcgaggag
     5341 tcggagcggc tcgccctcgg gcgcgtcacg gagtccggcg gcaggccgga cgaaccggcc
     5401 ctggagggcg tcggcgatct cggcatcggt cagggcgatc gacggcagcg cgtccaggcc
     5461 cgtgtcggat ggcagcagca gcgctttgac gccgtccggg cctgattcgg cggccgccga
     5521 tcggatctcg tcaagcgaac gagcgccctc gagggtgaac gggccgctcg cgagccgcac
     5581 gagggcgccg aggtaggcgc cgcagccgag gcgcgcgccc aggtcgcgag cgagcgcccg
     5641 cacgtacgtg cccgcggaac agcggacgtc gacgatggcg atcgggcgat cgggatccgt
     5701 gccgtcccac tcgagcaggt cgaggccctt gatctcgacg ctgcgcgagg gcaggtcgac
     5761 cgtctcgccg gcgcgcgcca tcgcgtaggc acgacggccg ccgacctgta ccgctgagta
     5821 cacaggtggc tgctggagct ggccgccgag gaagccccgg agcgcaccct cgacctcgtc
     5881 gcgcgaaggc gcaggagctt cgatcggcgt cagctcgcca tcgaggtcat ccgtagtcga
     5941 ggtcgccccg aagcagatcg tggcccggta ggccttgtcg tcggacaggt ggtactcgac
     6001 gaggcgggtc gcgccgccga ggaacagcgg cagcacgccg gacgcgaatg gatccagcgt
     6061 cccgccatgg ccgatccttc tcgtcgaggc gagtcgacgg accagggcga cgacgtcgtg
     6121 cgatgtcggt ccggccggct tggccacgat cagcacgccg tgcatgcccg gcgcgtcggg
     6181 ttgtcgggcc atggcgggat ctaggacgcg gacgagcgcg agacggcggc gacgaggcgc
     6241 tcggcctccg ccaggacgcg gcgtcgtgcc tctgcaagcg gcgcatcgac cgtcgccccc
     6301 gccgcccggg catggccgcc gccaccgaat gcgccggtga ggacggttgc atccacgcca
     6361 ccaggcttcg tgcggacgga cagccgggtg gaggcaggcc ccgcttcctt gaacaggatc
     6421 gcaacctcgg ccgtctccga ctgcgcgagg aggtcgatca ggccctccga gtgggaggcg
     6481 agcgtccccg tcgcggccag gtcggcatcg tcgagcgagc tgtggatgac ccggccgccg
     6541 tcggtgacca cgagccgatc gagcacacgg ccgaagagtc gcagctgggc atccggcttt
     6601 gagcggtaca ggcggcgcga gatctcggcc aacggcgcac ccgctgccac gagtgctgcg
     6661 gacacggcga gcgtgcgcgg cgtcgcgttc ggatgggcga acgtcgaggt gtccatcacg
     6721 atcccggcca tcagcgaggc cgccatcagc ccgttcgcgg cgtcgagggg aacgtcgagt
     6781 ttggcggcga ggagtgcgac catctcgcac gtcgcggcgg cggcggggtc gatccagtcc
     6841 ccggccctgc cgtcggtgtt cgaggcgtgg tggtcgacca ggacgcgcgg caggcgctcg
     6901 aacagctcgg gatgtcgctc gcgcacggcg cccagccgat ccagcgagcc gcagtcgcac
     6961 acgacgagca ggtcgtactc gccgtcggcg gcgggatcct gcacgaagcg gtccatgccc
     7021 tcgaggaagt cgtacagcgg cggcaccggg tccgtggaca ccagcgtggc gcggccgccg
     7081 tggtgctcga cgatcgctgc gatgcccagc gcggctccga gggtgtccgc gtcggggttc
     7141 tcgtgggtcg cgaccagcgc tcgccgggcc ccggcgatgg cggcggcggc cgcgtcgggc
     7201 acggcgtcga gccacggtcg gaggtcgatg ctcatcgtcg cgcaccccgg tcgcgcggtg
     7261 ctcgccgcgg atccctgcga cgacggggtg ctcgggcttc gggcatcggc ggcacctgcc
     7321 cgacgggcgt cggcagcgat tcctcggggg gtggcgcgtc cgcgacctcg ccgccggcct
     7381 cgagctccgc cagcagctgg agcacgcgcg tccctcgctg gaccgtgtcg tcgagccgca
     7441 gctggagctc ggggatgcgg cgcaggcgga cccgcgcgcc gagctcgcgg cggacgaagg
     7501 gcatcgcatg ttcgagcgcg cgccgcgtga cctcccgctc ggccggctgg ccgatcacac
     7561 tgacccagac ccgcgcaacg ctgaggtcgg gcgcggtctc gacgtcggtg acggtcacga
     7621 acccgatgcg cggatcctgc acatccctgg tcaggatggc gccgatctcc tggcgcagca
     7681 actgatcgat cttctctgtt ctcgccgtca tgccgtcact tcgagcctgt ctcgcgcccc
     7741 gaccctagga gagcgcggat cgcgagacca tctgcgtact gaagcactcg atgacgtcgc
     7801 cttcgaggat gtccgtcgtc gaaacaaggc cgatgccgca ctcgaagccg gtctggacct
     7861 cgcggacgtc gtccttgaac cggcgcagcg agtcaatctt gtcggtcacg atgaccttgc
     7921 cgccacgcca cagccgggcg ctgcccgagc ggacgatgcg gccgtcggtg acgtacgagc
     7981 cggcgatgac ctggcgcccg accttgatga gcttgcgcac ctcggcgcgg ccctcgatga
     8041 cctcgaccgc ctccggctcg aggaggccgc tgagcgcctt ctcgatgtcg tcggtgagct
     8101 ggtagatgat gtcgtacatc cgcacgtcga ccttctcgga ctcggccgcg cgccgagccg
     8161 tgtcgctgat cgcggtgttg aagccgacga cgatcgcgtt cgacgccgag gcgagcagga
     8221 tgtcgttgtc gctgatctcg ccggcgccct cgtacaggac gttgagcttg acctcgtccg
     8281 tcttgagctg gccgagggcg tgggtgatcg cgccgagcga gcccgagacg tccgccttga
     8341 ggacgatccg gagctccttg gcctgaccgg cctggatctg gcgatacagg tcctcgagcg
     8401 tcgcccggcc gctgccctcg ccgcccttcg cggcgatctc ggcggtgcga gcttcgacgg
     8461 ccgcgcgggc ggccttctcg tcggcgacga cacgcaggat gtcgcctgcc tccggcacct
     8521 gggacaggcc caggatgacc gccggcgtag cggggcccgc cttcttgatt cgcttgccgt
     8581 gctcgtcctc gagcgcccga acgcggccga acgtctcgcc cacgacgatg atgtcgccga
     8641 cgttgagcgt gccggtctgg acgaggacgg tgccgaccgc gccgcgggcc ttgtccatcc
     8701 gcgcctcgac gatcgtgccg atcgcggtgc gcttcgggtt ggccttgagc tcctggaggt
     8761 cggcgacgag caggaccatg tccacgaggt cctcgaggcc gacccggctc ttggccgaga
     8821 cgggcacgag cggcacgtcg ccgccgtact cctcgatgac gatgccggcc tcggtcagct
     8881 cggtcttgac gcgatctgga ttcgcgtccg gcttgtcgat cttgttgagg gcgatgatga
     8941 tcggcacctt cgccgccttg gcgtggctga tcgcctcgag cgtctggggc atcacgccgt
     9001 catccgccgc gacgacgacg accgcgacat cggtgacgcg cgcgccgcgg gcgcgcatcg
     9061 ccgtgaacgc ctcgtggccc ggggtatcga ggaacacgac gcggcgatcg ccacggtgga
     9121 cctcgctcgc gccgatgtgc tgggtgatcc cgccgcgctc gccggccgcg accgtggtcg
     9181 agcggatggc gtcgagcagg ctcgtcttgc cgtggtcgac gtggcccatg accgtgacga
     9241 tcggcgagcg atcgacgaga tccgcggtgt cgtcctcggt gaacagctct tccttcgtcg
     9301 cggcctgcgg cgcatcgacg ccgtcctcgc cggcgaccgg cacgccgggc gcctggggct
     9361 ccgcgacgtc gtagccgagc tcgctcgcca ccagcgacgc ggtgtcgcgg tcgatgagct
     9421 ggttgatcgt ggcgaagatg ccgctcttga tcagctcgcg gatgacatcc gccgggttca
     9481 cgccgaggac gtcggcgagc tccttgacgg tgatcgtcgc cggcagctcg accgcgcctc
     9541 ggtccttcgg atcgaggacc tggaccgcgg tattcgcaaa tgcgccgtcg cgacgcgggg
     9601 gcttgcgcgg accacggcgg ccgcgggtgc cgctcctact tcgagccatg agtgtctccc
     9661 ccgcgcacgg cggtcatcaa tgctgcagtg agttcggccg gcaggctcgc ttcgagggcg
     9721 cgaccgaggg cgccccggcc cgtcgctccc gtcacgcacg cctcgtcgcg gcacacgtag
     9781 gcgccgcggc cgttggcgcg gccgttcgga tcaaaggtga tggtgccgtc gggcgcccgc
     9841 acgacgcgca gcaggtcacg cttctcgcga ctggtgcggc agaagacgca ggatcgggtc
     9901 gggaccgctc gacgttcgag ggtcgccggt gccgcgccta cgacgtcacc tcggcggcgg
     9961 cgacctttgt cttgcgtgcc ttcggcttcg gagcctcggc gtcggccgac tcgacgggtg
    10021 tggcatcggg ctcgggagcg gcatcggcgg ttgctgtcgc gaccttcgcc ttcgtcgccc
    10081 gcggcttctt cggcgccttg acctcgatca cggcatcagt ggcagccgcg gctggcgttt
    10141 cggcctttgc agccttggtt gtcttgggcg ccttggcggc ctttgccggc gcctcggcca
    10201 ccgtcgctgc agccttgttg gccttcgcgg cctttgcggc ggcctttgcc ggcttctcgg
    10261 cggcatcggc aacgaccggc tctgccgcgg gcgcggcctc cgcctccggc gccttctcgg
    10321 cggcggcctt cgcctcggcg accgagacgt cgctgcggat gtcgatgcgc cagccggtca
    10381 gtcgggcggc gagccgggcg ttctggccct cgcggccgat ggccagcgaa agcatccgct
    10441 cgggcacggt gacgttggcg atgcggtgct ccgtgtcgat ctcgacggag atcacctggg
    10501 cgggcgacag cgcgttggcg acgaacgccg acgggtcgtc gttccagggg atgacgtcga
    10561 tcttctcgcc accgagctcg gcgacgaccg cctggacccg cgcaccgcgc tggccgacgg
    10621 tggcgccgac cggatcgagg ccctcctggc gtgacgcgac ggcgaccttc gagcggctgc
    10681 cggcctcacg ggcgatggcc cggatctcga ccgtgccgtt gtggatctcg gggacctcga
    10741 gctcgaacag gcgcttgagg aagcccttgt gggtgcggct cacgaacagc gcggggccgc
    10801 gcggggtgcg ccggacctcc aggacgtacg acttgacgtt ctggccgatt cggtaatgct
    10861 cgagcggcga ctgctcggtg gtggcgagga tgccctcgac gcccttgccc atgtccagga
    10921 tcatcgcccg cggctcgacc cgattgacct ggccggtgac gagctcgccc tcgcggtctg
    10981 cgaactgatc gaacacggcg tcgcgctcgg cctcgcggag gcgctggagg acgacctgct
    11041 tcgcggtctg ggcgccgatt cggccgaagg tatccccgtc cagctcctcg atctcgacga
    11101 gctcgcccgg gatcgcgtca ggcttgtggc gcttggcctc gtcgagggtg aagtgggtca
    11161 gctgctgctc ttcctcgatc gggtcgaccg aggcgatgcg cgctcgatac acgcggatcc
    11221 gaccgctgtc ggcgtcgatc tggacgtgga tgtcctcttc gttcgcgaca cgtcggtatg
    11281 cagcggcgat cgcatcctcg accgtgcgaa tgagctgctc gcgcgagacc tgcttctcgg
    11341 cgttgagctg gaggagggcg ccgacgaaat cgcgactcat ggcgttcgtc ctcctctcga
    11401 tgtcggccgc agccggacgt attccgccga ataaaaaacg tgggggtcac ccacgcttcg
    11461 aggcggccaa tttctgttcc gcggagtata cacgcgaccc cgtggaccgt cgatcagtgc
    11521 cggtcgcaag gccccgggtt ccatatcgca aggtggctcg ctttgcgccc gatggccctg
    11581 tcgtcaccca tctccttcgg acttcggcat tcgattggat cgagacaggg acggacgaag
    11641 cagctcgagg caacggtggg aggacagtcg aggctcagga tgtgggcgtc aggcgtccga
    11701 tatgcgatct gatggcgcct tgggagcgcg ggcgtatccc ttcacggtcc cccgacggct
    11761 tcgatcgtcg ttgctgagat tcgatcgcaa cttgaccaac gggcacgcgg tgagcggacg
    11821 ccagagggcg gacggctcgc ggaggaactg ccgaacgtcg cgctcgccta tgtccgaaat
    11881 gcaatcgtcg ccaggctcag gcgggagcga cgaccccagc tcctgagcgg gcgccgtagc
    11941 gctggtcgac gtaggcctcg aggatgccga tgaactcgtc cacgaggccc tcgccgcgga
    12001 gcgtgcggtc gagcttgccg tcgatgaaga cgggcgcaac cggctcctcg aacgtgcccg
    12061 gcagcgagat gccgatgtcg gcgtgcttcg actcgcccgg gccgttcacg acgcagccca
    12121 tgacggccac cgtcatcgac tcgacgcccg gatagcgcgc ctgccagccc ggcatctggt
    12181 cgcggaggta gccctggatc cgctcggcca tctcctggaa gaacgtcgag gtcgtgcggc
    12241 cgcagcccgg gcaggcactg acctggggca ggaaggagcg caggcccatc gactggagca
    12301 cctgctgggc gacccggacc tcctcctcgc gctcgccgcc cggagcgggc gtcagcgaga
    12361 cgcggatcgt gtccccgatc ccctcctcga ggagcacggc gagcgcggcg gtggatgcga
    12421 cgacaccctt catgcccagg cccgcctcgg tgaggccgac gtggagcgga tagtccgagc
    12481 gccgggcgag aatgcggtac acgtcgacga cgtcgcgcac gttcgagacc ttggccgaca
    12541 ggatgatcct gtcgtgcccg aggcccgtct cctcggccag ctcggccgag cgcatcgccg
    12601 actcgatcat ggcctcgatg atcacgtcgc ggctctcgcg cggctcggcg agcttcgcgt
    12661 tctcgtccat gagcaaggtc agcagggcct gatcgagcga gccccagttc acgccgatcc
    12721 gcaccgggac gtcgttctcg accgccacgc ggacgatcgt ctggaagttc tcgtcatgcc
    12781 gcttcgcgcc gacgttgccg ggattgatcc ggtatttcgc caggccgcgg gccatctccg
    12841 ggtactcgac caggagctgg tgcccgttgt agtggaagtc gccgatgacc ggcacgccga
    12901 cgccgaggtc gcgcagcttg cggagcatgt cggggacggc ggcggcggcc gcgtcgttgt
    12961 tcaccgtgac gcggaccagc tcgctgcccg cgtgagccag gcgagcgacc tggatcgccg
    13021 tcgcgtccgc gtcggcggtg tccgtgttgg tcatcgactg gacgacgacc ggatggtcgc
    13081 tgccgaccat cactccgccg acgtcgacgg tgacggtctg gcggcgaacc ggggcgctca
    13141 tgggcaggcc gcggctacgg cgccgagccg aggcgcgcga tgtcgaagcc cgatatccac
    13201 agcacgaagg ccatgaggaa caggaatcca accgcgtagg tcaaccgctc ggcgcgcagg
    13261 ctcacccgcg ccccgaacac gcgcttgacg acgagcacgg ccatgcggcc gccgtccagt
    13321 ggcgggaacg gcaggatgtt caccacggcc aggttcaccg acaggatgcc cgcgacatac
    13381 aggagccaga tcgggccggc gttcagcagg atgtcggcga gcgagacggc gatgccgatc
    13441 ggaccggccg ccggcggctc cgcggtcggg tccttgatga agccgttgac gaggtcgccg
    13501 agccctcgga agatcagccc gccccagcgg gcgacctcgc tcgaggcgat gcccaccgac
    13561 gccccgaggt cgtggctggt ataggcgttc gtctgttccg tgctcacgcc gagcgcgccg
    13621 cgcgtcgggc tcagctcgga tttcggtcgg agcgcgaccg acacgtcgac ctcggacccg
    13681 tccgggtggc gaatgccgat cgtgatcgtt tcgccgccgt gccggcggag cgcctgggtc
    13741 gcgctgtcgc cgctgaacag ctcgaactcc tggccggcca catggtagat ctcgtcgcct
    13801 ggctggaggc cggcagcggc cgcgggcgag ccggtttcga ccgagacgat gcgggcgccg
    13861 agcaccggcg tcgcgagcag ggcgatcagg aagaagatcg cgaaggccag gacgatgttc
    13921 atcacgacgc cggcgacgag gatcgcgagc cggaccttga gcgacttggc gccgaacgag
    13981 cgcgggtcga ggacctcgtc gccgtcctcg ccgtccatcc gcacgaagcc gccgatcggc
    14041 agccagttca gcgtccagag ggtctcgccc ttgttgcgca gcaccttggc gcgcggcggg
    14101 aagccgatcc cgaactcgag gacgcggacg ccgaggcggc gggcggtgat gaagtggccg
    14161 agctcgtgca cgaggacgat gcccccgagc acgacgatga acagcaggac cgcgagcggc
    14221 ccgtagagca ggccatccat caggcggtca cccgtgtacc ggcgccggcc cctgcgagcg
    14281 atcggcggac ctcgaggtcg agggcgacga gctcctcgac gttcgggtct gcgcccgcgg
    14341 cagtgccgaa tctggccact gcatcctcga gcaggcgcgg gatgcccggg aagtcgagcg
    14401 tgccgtcgag gaagcgagcg accgccacgt catccgcggc gatgagcgca gccgtggcgc
    14461 gcgggccgag cgcccccgcc tcacgcgcga tccggagcgc cgggaagcga gtctcgtccg
    14521 gggcgcggaa gtcgaggcgt gcgatcgcga tgaggtccgg cgccgcggcc ggcgagagcc
    14581 ggcgatcggg ccaggtgagc gcatattgga tcgggagtcg catgtcgggg gtgcccagct
    14641 gtgccttcag ggagccgtcg acgaactgga cggcagagtg cacgaccgac tgcggatgga
    14701 tcacgacgtc gatggcctcg taggcgacat cgtaaagcca gcgggcctcg atgacctcga
    14761 gacccttgtt cgcgagcgtc gcggagtcga tcgtgatctt cgcgcccatg gtccaggtcg
    14821 ggtggcggag cgcgctcgcc ggggtaatgg ccgcgaagtc ggcggcgggc aggtcgagga
    14881 acggcccgcc cgaggcggtg aggatcaacc gctcgacacg ggcgaggtcc tcgcccatca
    14941 gcgattgcca gatcgcggag tgctcggagt cgatcggtcg cagccagcca agcgggcttg
    15001 ccaatgggtc ggcaggatcg atggcggctc gtcgggcggc gtgtgcccgg gcgaggggca
    15061 tcacgaggtg gccgccggcg acgagcgtct ccttgttggc cgtggccacg atcttgccgg
    15121 cctcgagggc ggcgagcacg ggtcgcaggc tgaccacgcc gccggtcgcg acgacgacca
    15181 ggtcgacgtc gtcgcgaatc gccagctcga tcagggcatc gtcgccgcgc atgaccgtgg
    15241 tgccattcgg caggccgtgc tcgaacgccg ggtggtccgg ggcgtcctcg gccagggcga
    15301 cagccaccgg ccggaagcgt tcgacctgct cggccagcag ggcctggttg cgcccggcgg
    15361 caagggcgac gacccggaat gccgggtcgc cggcgaggac gtcgagcgcc tggcgcccga
    15421 tcgagccggt cgagccgagc agggcgatgc cgcgcggtcg gttgtcacgc ggctcgctca
    15481 cggcaggatc agtggagaat gctgacgaca tagaaatacg ccacgggcgc ggcgaacagg
    15541 aacgaatcga cccggtcgag cagaccaccg tggcccggga tgaggcggcc cgactccttc
    15601 gcgcccgccg cgcgcttgag catcgattcg gcgaggtcgc cggcctgcgc ggctgccgcg
    15661 atcgcgacgc cgagcacgag cccggcgagc catggccggc cgagcagggt gacgaccacc
    15721 gcgccgacga ccgccgccgc cgccatgccc ccgatcacgc cctcgacggt cttcgacggc
    15781 gagatgtggg ccatgaacgg ccgccggccg aagcgcttgc cgacgaagta ggccaacgtg
    15841 tcgaacgccc agacgacgag cacgaggacg acgatccatg cccgctccgg gccgagccag
    15901 ccgaggatcg agcgtgggtc gacggccgag tccgtggacg cgaggcgcgc gatgaagccc
    15961 agcagcccga catacagggc gccgaaggtg gtcgtggcga agatcgcgag gccctcgcgc
    16021 gggtccagcc gggtcagcgc cgcggtcccg atcaggatga tcccgagtcc ggcgagcagc
    16081 aggccggagc cgccggggac ctgcttgacc ccgtcaccaa gggcgacgac gacggcaagc
    16141 acgatgccga agagcggcat cgagtgaaac ccggcagcgg tcaggagctt gaacgcctcg
    16201 tagcccgcga ccacgacgac cgcggcgacg accaggacga tggcccagcc accgagccac
    16261 atggcgacca gcagtggtgg gaccaggatc gccgcgctgc gggcgcggtc gcgcagcatc
    16321 cggtcagcgc ccgaagcgac gcgtacggct ggcgtactcg gccagggcca ggtcgagatc
    16381 ggccggcccg aaatcgggcc agagcgtgtc gctggtcatc agctccgcgt aggcggattg
    16441 ccagatgagg aagttcgaga gacgctcctc gccaccggtt cgaatgatga gatccggatc
    16501 gggcaggccg gcggtgtaga gcgaggccga cacggtggct tcgtcgatcg cctcgggcgg
    16561 cagcccggcc cggacgatgt cccgcaccgc gtcgacgagc tccgtgcggc ccgcgtagtt
    16621 gaacgcgatg ttcaggacga gccgatcgcc gccggcggtc gcggccagcg ccgactcgat
    16681 cgaggtgcgg gtcgcggttg ggagctcctc gagacgaccc agcagtcgca cgcggacgcc
    16741 ctgcgcctgg agctcggcgg tctcgctgcg gatcgcgctc tcgagcaggc cgaacaggcc
    16801 gacgacctcc tcgtccgtgc gcgcccagtt ctcccggctg aacgcgtaca gcgtcagcac
    16861 cgggatgccg acgcgaacag cgtggcgcag gagcccgcgg atcgcctcga cgccggccgc
    16921 atggccctcg atctcggaga cggaacgggc gcgggcccac cggcggttgc cgtccatgat
    16981 gatcgcgacg tgggtcggcg cctcggcgcg gtcgccgcgc ttgccggcga cgacctccgg
    17041 gcgctcgatg ggaagccctg ccgcctcgac ttcgtgcgcc cgtggcggcg cgtcggtcgg
    17101 gcgaatcagc ggcgcacgcg ccactagacc tcgaggacct cctgctcctt cgccgcgccg
    17161 acccggtcga cctcggcaac gtggcgatcg gtggtcttct ggagtgactc gaggagcttg
    17221 tgctcctcgt cgacgccgac ctcgccctcc ttctggagct tcttgaggtg atcggcggcg
    17281 tcacggcgga ggttgcggat ctcgacccgc gcctcctcca tgcgcttgtg gacctgcttg
    17341 accaggtcgt ggcgccggtc ctcggtgagg agcgggatgt tgagccggac cacggtcccg
    17401 tcgacgttcg gcgtcaggcc gatgtcgctc ttcaggatcg cccgctcgat ggcgccgagc
    17461 gcgctccggt cccacggctg gatcacgatc tggtgcggct ccgggacgct gatcccggcg
    17521 agctggttca gcggcgtctg ggtgccgtag taatcgatgt ggatgcgctc gacgagcccg
    17581 gtcgacgcgc gaccagtccg gatcgagttc aggtcgcggt ccatgacctc gacggcgcgg
    17641 gccatcttgt ggtcggccgt cgtgatgatc tcgttgctca tgcgcgaact ccgacggggc
    17701 ccgcgatcag cgtgccgatc gtctcgccgg cgacgacgcg ggcgatgttg tccgtgccgt
    17761 tgaggtcgaa gaccacgatc ggcaggtcgt tctccatgca cagcgagacc gccgcgccgt
    17821 ccagcacctt gagctggtcg cgcaacaggt cggcgtactc gagccgttcg tagcggtggg
    17881 cgccgggcac ggtcatcgga tcggcatcgt agaccccgtc caccttggtg gccttcatga
    17941 ggacctcggc gccgatctcg acggcgcgca gcgcggcggc cgtatcggtc gtgaagtacg
    18001 gattgccggt accggccacg aagatcacga cgcggccctt ctcgaggtgg cgcaccgcgc
    18061 gccgccggat gtagggctcg gcgatctcgt tcattgcgat ggcgctcatc acgcgggtcg
    18121 gtgtctccgt gcgctcgatg gcgtcctgga gggccaggcc gttcatgacg gtcgcgagca
    18181 tgccgatgta gtcaccggtc gcgcggtcca tgccctgcgc ggccgcggac aggccgcgga
    18241 agatgttgcc gccaccgacg acgatgccga cctcgacacc cgtggcgcgg acggccgcga
    18301 cctgccgggc gatgaaggcg cagacctcgg ggtccacgcc gtactggcgc gagcccagca
    18361 gggcctcgcc ggacagcttc agcaggatcc ggtggtagcg cggcgcgccc gacgcgtcgc
    18421 tcacttgtcc tccccgaggg cgtagcgctc gaagcgccga acctggatgt tctcgccgat
    18481 cgtcgctacg gcctcggtga cgagggcgcg gacggtgccc tcggtgtcgc gatacggctg
    18541 ctcgagcagg acgccgcccg gctccgcgta ccacttcttc agccggccat cgacgatctt
    18601 ctcgcggact tcggcgggct tggccgcgac atcggtcgcg tccagcagct cggtcttctt
    18661 cgcggcgagg acgtcggcgg ggatatcggt gatgtcaacg aagcgcgggt aggtcgcagc
    18721 aacctggacc gccaggttgc ggacgagcgc ctggaactgc tccgtgcggg ccacgaagtc
    18781 cgtctcgcag ttgacctcga tgagcacgcc gacgcggccg ccggtgtgga tgtagctgga
    18841 gacgaggcct tcgcgggcct cgcggccggc cttcttggcg gctgcggcaa gcccgcgctc
    18901 gcgaaggatc gcgatggcct tgtcgacgtc gccgtcggtc tcatcgaggg cgcgcttgca
    18961 gtccatcatt ccggcgcccg tgcgctcgcg aaggttcctg accgcctctg cggtgatgtt
    19021 ggccatgtgg gtgctcgtgc tcctgttcgt gctcggcggg ctcaacgggc tcgcggtgtg
    19081 gtggtcggtt ggttggcgtg gtccttggga ccggccgggg tcaggcgggg gtcgggtcgc
    19141 cctcgcgggt cgcggcctcc gcggcctcgc gcgccagctc ggcggcgatc tcctcgggcg
    19201 tcgctgcgtc ctccgggaca gccgggatca ccggcgcgcc ggggaggagg tcgtcgtcat
    19261 catctggcgc gaagctgagc gcggcgccgc cggcgagggc tgcaacgagc tcgtcggtcg
    19321 ccgcctcggt gtcctcgtag gtcggctcat cgacgatctc gacctcgggc tcgatcgccc
    19381 ggctcgcccg gtcctgggcg ccctcgatcg ccgcgtcggc gacgagcgag cacagcaggc
    19441 ggatcgcgcg gatcgcgtcg tcgttggccg ggatgatgta gtcgagctcg tcggggtcga
    19501 cgttcgtgtc gcccgtgccg acgaccggga tctcgaggcg gttggcctcg gtcacggcga
    19561 tccgctcgcg gtgcgggtcg acgatgaaga tcgcaccggg caggcgcttc atcttgcgga
    19621 tgccgccgag gatcagctgg agtcgggcga gctcctcgag gagcttggcc gcttccttct
    19681 tgggcagtcg atcgaggtcg ccggcctgct gccgcgcctc gagctggtcc atcaggccga
    19741 tgcgcttctt gatggtcacg aagttggtca gcatcccacc gagccagcgc ttgttgatga
    19801 agggcatgcc ggcgcggatg gcctcggccg cgagcggctc ctgggcctgc ttcttggtgc
    19861 cgacgaagag gacgacctcg ccgcgggcga cggtctcgcg cacgaagtcg agcgcggtct
    19921 ccagacgctt cgcggtctgg gcaaggtcga tgatgtggat ccc
//
LOCUS       DATBSW010000060         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1204010, whole genome shotgun sequence.
ACCESSION   DATBSW010000060 DATBSW010000000
VERSION     DATBSW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204010"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VIF63_01595"
     CDS             complement(<1..447)
                     /locus_tag="VIF63_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238103.1"
                     /translation="MANRVGRDGGIDDPNIREGGASNPGNAADELMIHDVDNRPVDEG
                     GADRPPTPGSDSGIVINWKPFAVGAIVVGVVVVSAFTFLKPGAAPGSSVAPGSSPAAG
                     ATSGVVVPGASGFNGPARAYVTVNGTLHYLTGGFCTRQATDFGPDSA"
     gene            complement(552..1361)
                     /locus_tag="VIF63_01600"
     CDS             complement(552..1361)
                     /locus_tag="VIF63_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238104.1"
                     /translation="MTLQGRSLDDLPLAAYTTGMQPDPRLEDAEAASSSSPEPATHLA
                     AAVKAPGDHLDLSTEPAGAGAATAGRLGRGMPALPAMPALPALPAWLAHPRQHLREPR
                     LLLTGVVTVGVVLLAVSLLGGGAGPLGAAAASPTPGAPVVAATLAPPTGDASVEVTGK
                     TTGSYTLVGRTGTGPAVASHIDATWGDVSGTALRLAGIATPGTRLTDGTFSLTWTIMV
                     DGAPVIFTSEAGECTVGMAVQPKSVTGSFVCKQLKSADGKLVVDAKGTYKT"
     gene            complement(1446..2645)
                     /locus_tag="VIF63_01605"
     CDS             complement(1446..2645)
                     /locus_tag="VIF63_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016220512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8238105.1"
                     /translation="MTTEATRPPLAERVLAERVHQVPPSGIRKFFDIVATMSDVISLG
                     VGEPDFDTPRSIVEAGVESLREGRTHYTANAGTLELRKALADHLERLYGVRYDPKTEL
                     MITVGASEGLDLALRATCEDGDEVILHEPSYVAYVPAIVFAGGRTVHVATRFEDDFAL
                     DPADLEAAITPRTKAIFLGYPCNPTGAVLSDANQEAIATIAERHDLLVYSDEIYDRLA
                     YGSYRHRAFSALPGMKERTILMGGFSKAYAMTGWRIGYIAAPPQILEGMLKIHQYTIM
                     TAPTIAQDAALVAILEGEPDVERMRLEYDRRRRLVVDGLNAMGLETFEPRGAFYAFPR
                     ITSTGLDSETFARRLLEEERVAVVPGGAFGPSGAEHVRACYATSYEQLQEALVRIGRF
                     VERVRSD"
     gene            complement(2655..3920)
                     /gene="glyA"
                     /locus_tag="VIF63_01610"
     CDS             complement(2655..3920)
                     /gene="glyA"
                     /locus_tag="VIF63_01610"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8238106.1"
                     /translation="MTPPGDRTLARTDPEIARLVEAERVRRGRTLDLIASESEPSPLV
                     LEALGSVFAAKTAEGYPGRRYHRGTEFTDQVEQLAIDRAKAVFGAEHANVQPSSGVNA
                     NLAVYRALLKPGDAVLALQLAHGGHLSHGDPASITGAVYQFAHYGVRIDTETIDLDEV
                     RDLARRHEPKLIVTGGSSYPRAIDYAAFAAIAREVGARLLVDMAHVAGIVAAGLLPSP
                     VPHADAVTFTTYKTMLGPHGGVILTTAELARKIDRAIFPGTQGAPDFGHVAAKAVCLG
                     YAATDAFRATQAAILANAAALAAELARLGDRIVSGGTDNHMVLVDLRGRGLTGDVAEV
                     ALEAVGILANRNVIPFDPGTPDRPSGLRLGATSSAQRGIGPPEMLEIAALVDATLRTA
                     TDTTNPASDELKTRVEDLAARFPSPAAAR"
     gene            complement(3917..>5062)
                     /locus_tag="VIF63_01615"
     CDS             complement(3917..>5062)
                     /locus_tag="VIF63_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY8238107.1"
                     /translation="GPVSNPWDLDRVPGGSSGGSAAAVAAYHAPFGIGTDTGGSIRQP
                     AALSGIVGLKPTYGRVSRYGIVAFASSLDQIGPLARDARDAAVLLHAVAGRDARDSTS
                     APVPVPDDLLRLPAGDDEAASWLKGKRLGLPAEYFVKGMEPGVEARIREAVDQLEAAG
                     AEVREVSLPHTDYGLATYYIVVPAEASANLARYDGVRYGHSVRLPGADFIADYLATRG
                     EGFGPEVKRRIMLGTYALSAGYYDAFYLKAQKVRTLIKRDFDDVFESGIDALVAPTSP
                     SVAFTFGSKTADPVAMYLSDACTLPANMAGIAGVSLPCGLSEGLPVGLQFIGPPWSEL
                     ELLRFGRAYEAITADADWRRLEPAQLALTDDQSGPTPTQRAAALAAA"
BASE COUNT          721 a         1726 c         1867 g          748 t
ORIGIN      
        1 tgcggaatcg gggccaaagt cggtggcctg cctcgtgcag aaacctccag tgaggtagtg
       61 gagcgtcccg ttgacggtga cataagctcg tgccgggccg ttgaacccgc ttgcgcccgg
      121 cacgaccacg ccactcgtcg cgccggcggc cggactggac ccgggagcca cgctggaccc
      181 aggagcggcg cccggcttca agaaggtaaa ggcagacacg accaccacgc ccacgacgat
      241 tgcgccaacc gcgaagggct tccaattgat gacgatccca gagtcgcttc ccggcgtcgg
      301 cggccgatca gcgccgccct cgtcgacggg ccggttgtcg acgtcgtgaa tcatcagctc
      361 gtcggcggca ttgccaggat tgctggcgcc gccctcgcgg atgttggggt cgtcgatgcc
      421 gccatcgcgg cccactcgat tcgccatccc gcgtcctcct cggggtcccg gcttccccgc
      481 aaggtacagg atgttggggg tcgtgttcga cccgtgaagt gttcaggcgc cgacgccgag
      541 tccgacggct cttaggtctt gtatgtcccc ttggcgtcga cgacgagctt gccgtcggcg
      601 ctcttcagct gcttgcatac gaagctgccg gtgaccgact tgggctggac ggccatgccc
      661 accgtgcact cgcctgcctc ggacgtgaag atgaccggtg cgccgtcgac catgatcgtc
      721 caggtcagcg agaacgtacc gtccgtcaac cgcgtccccg gcgtcgcgat gccggcgagg
      781 cggagcgccg tgcccgagac gtcgccccag gtcgcgtcga tgtgcgaggc gaccgcgggg
      841 ccagtgccgg tgcgcccgac gagggtgtac gagccggttg tcttaccggt gacttcgacg
      901 ctcgcatcgc cggtcggcgg cgcgagcgtg gctgcgacga ccggcgctcc tggcgtcggc
      961 gacgcggccg cggcaccgag cggcccggcg ccgccgccga gcaggctcac ggcgaggagg
     1021 acaacgccga cggtaacgac gccggtcaac aggagtcgcg gctcacgaag gtgctggcgc
     1081 ggatgggcga gccatgccgg gagggcaggc agcgccggca tcgcgggcag cgcgggcatg
     1141 ccgcggccga gccggcctgc ggtcgccgcc ccggcgcccg ccggttcggt ggacaggtcg
     1201 aggtggtcgc ccggcgcctt cacggcggcg gcgagatgtg tggccggctc tggcgagctc
     1261 gacgaggcag cctccgcatc ctcgaggcga gggtcgggct gcatcccggt cgtgtaggcg
     1321 gccaacggca gatcgtcgag cgatcggccc tggagcgtca tgcgcaaatc ccctttcggc
     1381 acgcccaagg cgcaccacga gggatcgtct cgatcgacgc cccgtgcgtc aatgcaccaa
     1441 gcgtcctagt cggatcggac cctttcgacg aaccggccga tgcgaacgag ggcttcctgg
     1501 agctgttcgt acgaggtcgc gtagcaggct cggacgtgct cggcgcccga tggcccgaag
     1561 gcgccgccgg gcacgaccgc gacccgctcc tcctcgagga ggcggcgtgc gaacgtctcc
     1621 gagtcgaggc ccgtcgacgt gatgcgcggg aaggcataga aggcgccacg gggctcgaat
     1681 gtctcgaggc ccatcgcgtt gaggccatcg accacgagtc gacggcggcg gtcgtactcg
     1741 agccgcatcc gctcgacgtc gggctcacct tccaggatcg ccacgagcgc ggcgtcctgg
     1801 gcgatcgttg gcgcggtcat gatcgtgtac tggtggatct tcagcatccc ttcgaggatc
     1861 tggggcggag ccgcgatgta gccgatccgc cagccggtca tggcataggc cttcgagaag
     1921 ccgcccatga ggatcgtccg ctccttcatc cccggcaggg ccgagaaggc acggtggcgg
     1981 tacgatccgt aggcgagccg gtcgtagatc tcgtcgctgt acacgaggag gtcgtggcgc
     2041 tcggcgatcg tcgcgatcgc ttcctggttg gcgtccgaga gcacggcgcc ggtcgggttg
     2101 cacgggtagc cgaggaagat cgccttcgtg cgcggcgtga tcgccgcctc gaggtctgcc
     2161 ggatcgaggg cgaagtcatc ctcgaagcgg gtcgcgacgt ggaccgtcct gccgcccgcg
     2221 aacacgatcg cgggcacata ggcgacgtac gacggctcgt gcaggatcac ctcgtcgccg
     2281 tcctcgcacg ttgcccggag tgcgaggtcg agcccctcgg acgccccgac cgtgatcatc
     2341 agctcggtct tcgggtcgta cctgacgccg tacagccgct cgagatggtc ggccagcgcc
     2401 ttccggagct cgagcgttcc cgcattggcc gtgtagtgcg tccgtccttc ccgcaacgac
     2461 tcgacgccgg cctcgacgat cgagcggggc gtatcgaagt ccggctcccc caccccgagg
     2521 ctgatgacat cggacatcgt cgcgacgatg tcgaagaact tgcggatgcc cgatggcgga
     2581 acctggtgca cgcgctcggc gaggacgcgc tcggcgagcg gcgggcgggt cgcctcggtg
     2641 gtcatggcgg cagcctagcg cgccgctgcc ggcgacggga accgggcggc gaggtcctcg
     2701 acccgcgtct tgagctcgtc cgaggcgggg ttcgtcgtat ctgtggcggt gcgcagcgtt
     2761 gcatcgacca gggcggcgat ctcgagcatc tctggcgggc ctatgccgcg ctgggccgag
     2821 ctggtggcgc cgagacggag gccgctgggc cggtccggcg tgccggggtc gaacgggatg
     2881 acgttgcggt ttgcgaggat cccgaccgcc tcgagggcca cctcggcgac atcgccggtc
     2941 aggccgcggc cgcgcaggtc gacgaggacc atgtggttgt ccgtgccacc gctgacgatc
     3001 cggtcgccaa ggcgcgccag ctcggcggcg agggcggccg cattcgccag gatcgcggcc
     3061 tgggtcgcgc ggaacgcgtc cgtcgccgcg tacccgaggc agaccgcctt ggccgcgacg
     3121 tggccgaagt cgggcgcgcc ctgcgtcccc gggaagatcg cccggtcgat cttgcgtgcc
     3181 agctccgcgg tcgtgaggat cacaccgccg tgcggcccga gcatcgtctt gtacgtcgtg
     3241 aaggtcactg catccgcgtg cggcacgggg ctcggcagga ggcccgccgc cacgatgccg
     3301 gccacgtggg ccatgtcgac caggagccgc gcgcccacct cccgggcgat ggccgcgaac
     3361 gcggcgtagt cgatcgcccg tgggtaggag ctgccgccgg taacgatgag cttgggctcg
     3421 tggcgccggg ccagatcgcg aacctcgtcg aggtcgatcg tctcggtgtc gatgcggacg
     3481 ccgtagtggg caaattggta cacggcaccg gtgatcgacg ccgggtcgcc gtgggagaga
     3541 tgcccgccgt gcgcgagctg cagggcgagg acggcgtcgc cgggcttgag cagcgcgcgg
     3601 tacacggcga gattcgcgtt gacgcccgag ctcggctgga cgttcgcgtg ctccgcgccg
     3661 aacacggcct tcgccctgtc gatcgccagc tgctcgacct ggtcggtgaa ctccgtgccg
     3721 cggtggtagc gccggccggg atacccctcc gcggttttcg cggcgaagac ggacccgagc
     3781 gcctcgagga ccagtggcga gggctcgctc tcggaggcga tgaggtcgag ggtccgtccg
     3841 cgacgcaccc gctccgcctc gaccagtcgc gcgatctccg ggtcggtccg ggcgagcgtg
     3901 cggtcgcccg ggggcgtcac gcggcagcga gcgccgccgc tcgctgcgtc ggcgtcgggc
     3961 cggattggtc gtcggtgagg gcgagctggg cgggttcgag gcggcgccag tccgcgtccg
     4021 ccgtgattgc ctcgtacgcc cggccgaagc gcaagagctc gagctccgac cagggcgggc
     4081 cgatgaactg gagcccgacc ggcaggccct cggacaggcc gcacgggagc gagacgccgg
     4141 cgatcccggc catgttcgcc ggcagcgtgc aggcgtcgga caggtacatc gcaacgggat
     4201 cggcggtctt cgagccgaac gtgaacgcga ccgagggcga ggtcggcgcg accagggcat
     4261 cgatgcccga ttcgaacacg tcgtcgaagt cacgcttgat cagcgttcgg accttctgtg
     4321 ccttgaggta gaacgcgtcg tagtagcccg cggacagcgc gtacgtgccg agcatgatcc
     4381 ggcgcttgac ctcgggcccg aagccctcgc ctcgcgtcgc gaggtagtcg gcgatgaagt
     4441 cggcacccgg caggcggacc gagtggccgt agcgcacgcc gtcgtagcgg gcgaggttcg
     4501 ccgaggcctc cgcgggcacg acgatgtagt acgtcgcgag gccgtagtcg gtgtgcggca
     4561 gcgagacttc cctgacctcg gcgccggccg cctcgagctg gtcgaccgcc tcgcggatcc
     4621 gagcctcgac gccgggctcc atgcccttca cgaagtactc ggccggcagg cccaggcgct
     4681 tgcccttgag ccagctcgcg gcctcgtcgt cacccgcggg caggcggagg aggtcgtcgg
     4741 gcacgggcac gggggcagac gtggagtcac gtgcatcgcg cccggcgacg gcgtgcagca
     4801 gcaccgcggc gtcgcgcgca tcacgcgcga gcggcccgat ctggtcgagc gacgaggcga
     4861 acgcgacgat cccgtaccgg ctcacgcgcc cataggtcgg cttgagcccg acgatgcccg
     4921 agagcgcggc gggctggcgg atcgagccgc cggtgtccgt gccgatcccg aacggagcgt
     4981 ggtacgcggc caccgcggcg gccgagccac cgctgctgcc gcccggcacc cgatcgaggt
     5041 cccacggatt gctgaccggc cc
//
LOCUS       DATBSW010000061         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_851305, whole genome shotgun sequence.
ACCESSION   DATBSW010000061 DATBSW010000000
VERSION     DATBSW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851305"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VIF63_01620"
     CDS             <1..870
                     /locus_tag="VIF63_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238108.1"
                     /translation="VQAFEGSLEDWLGSFGGLVIGVSHEGGTWATNRALATARASGAT
                     LALLTVSDRSPGAAIADIVVATEELDQSWCHTVGYLSPMVAAIAVAGHLVAAPVAPAA
                     ARALLDAALTPAAVGATEAMAANLAGVQRLIVVATGVDRPAARELVLKVEEGAHLPAA
                     MRDVETLLHGHLAGMDAQTGLVLILTESAQAEARASRATGVLRAAAELGIPAGAILSE
                     RHAEAVPARLTPAGRLVVPAAAGLTPAAASLLGSAVPLQLLTERLARARGVDPDPIRR
                     DDPAYLRAADAAG"
     gene            complement(882..1880)
                     /locus_tag="VIF63_01625"
     CDS             complement(882..1880)
                     /locus_tag="VIF63_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8238109.1"
                     /translation="MRFALMIEAQMGLSYDDQLAIARRAEAAGFEAMFRSDHYAAFPG
                     DPGRGSTDTWAVLAGLVRETSKIRLGALVSPVTFRHPGNFVKLVTTVDHMSGGRIEVG
                     VGAGWNQEDHDPLGIPYPEMGARADWMEDQLELLLGLWTEPDGWDYDGHHVKVRGGAL
                     HPRPVDAPDRPHGRPGGSGEGPARPRIITGGQGTPRGFRLAAAYADEFNLSSSSPATA
                     KAKMAELDAACRAAGRDPSTLTRSSMCGVLLGADEAGVRRNADAIRVEFADSSGDTDA
                     WIKGRRGRWIVGTPDEARATIRGYAEAGIERIMLQDFLPRDLDYIDLLARELIGRV"
     gene            1976..2494
                     /locus_tag="VIF63_01630"
     CDS             1976..2494
                     /locus_tag="VIF63_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8238110.1"
                     /translation="MRSIVQPGTAVPAGDSADVETILTEVSGWVRELRCASMGRMVQG
                     KVSMSNMHVLWLLQHQGEMPMSRLADLLDVSLSNATGIVDRMEERGLVERVRVADDRR
                     IVLVRPAAAGMLALSETETTKRERMRAVVRRLPATERPVVLAALRSLRRALSAEVESA
                     PTHEHHFADAAN"
     gene            2531..>4049
                     /locus_tag="VIF63_01635"
     CDS             2531..>4049
                     /locus_tag="VIF63_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY8238111.1"
                     /translation="MESFTPEGPETPSIAEDPALGLSRRTKFEILGAVLLGMFLSALD
                     QTIVGPVLPKIVGELNGSDYYTWAVTIYLLTSTVTVPIYGKLSDLYGRKPVLIAGITL
                     FLAGSALSGLSQEMWQLVLFRGVQGLGAGALFPIALAVIGDLFTPAERGKYQGLFGLV
                     FGVAFLLGPAIGGTLTDTFSWHAIFYVNLPIGAIALFVIWRLLPNIKHTERAGKIDYL
                     GVVTLTLGLVPLLIGFTVAETTGFGDPFFWLWAGAGLVFLAIFVMVERRSPEPVIPLH
                     LFRNRTFSSSMLSIFFATFGFGAVIIFLPLYFLIVEGATATESGYRLLPFMFGLILAS
                     IASGQIVSRTGRYKPVVLVGLVTLIVGMALMTQLRAGTSDLVLAAWMFVAGLGVGPTF
                     AVFTIIVQNAVPFHELGTATSDLTLFRQIGTTIGIAVAFTIFRLNFTWDLIKEQLGAA
                     GVPAAFIPAAPPAGFDPGALTNPSGGGAAFDFMKLVPEEVRPLILDGIHRALTISI"
BASE COUNT          558 a         1518 c         1328 g          645 t
ORIGIN      
        1 gtgcaggcat tcgagggctc gctcgaggac tggctcggca gcttcggcgg cctcgtcatc
       61 ggcgtcagcc acgaaggcgg gacatgggcg acgaaccgtg cgctcgccac cgcccgcgcc
      121 tccggcgcga cgcttgccct cctgaccgtc tcggaccgct cgcccggcgc ggccatcgcc
      181 gacatcgtgg tcgctaccga ggagctggac cagagctggt gccacacggt cgggtacctg
      241 tcgccgatgg tcgccgcgat cgccgttgcg ggccacctgg ttgccgcacc ggtggccccg
      301 gccgccgcgc gggcgctcct cgacgcggca ctgacgcccg cggccgtcgg cgcgaccgaa
      361 gccatggccg cgaacctggc aggggtccag cggctcatcg tcgtcgcgac cggtgtcgac
      421 cggccggcgg cgcgcgagct cgtgctgaag gtcgaggaag gcgcccacct gccggcggcg
      481 atgcgcgacg tcgagacgct gctccacggc cacctcgccg ggatggacgc ccagaccgga
      541 ctggtgctca tcctgaccga gtccgcccag gccgaggcgc gcgcctcgcg ggcgaccggc
      601 gtgctccgcg cggccgccga gctgggcatc ccggccggcg caatcctgag cgaacggcac
      661 gccgaagccg tgcccgcccg cctcaccccg gcaggtcgcc tcgtcgtgcc tgccgcggcg
      721 ggcctgacgc cggcggccgc gtcgctcctg ggcagtgcgg tgccgctgca gctcttgacc
      781 gaacgtctcg ctcgggcgcg cggcgtggac cccgacccga tccgccgcga cgacccggcc
      841 tacctccgcg cggccgacgc tgcgggctga cccccggcga gctagacgcg cccgatcagc
      901 tcccgggcca gcaggtcgat gtaatcgagg tcgcggggca ggaagtcctg cagcatgatc
      961 cgctcgatgc cggcctcggc gtagcctcgg atcgtcgcgc gtgcctcgtc cggcgtgccg
     1021 acgatccagc ggccgcgacg ccccttgatc caggcatcgg tgtcgcccga cgaatcggcg
     1081 aactcgacgc ggatcgcgtc cgcgttcctg cgcacgccgg cctcgtcggc gcccagcagc
     1141 accccgcaca tcgacgagcg cgtgagcgtc gacggatctc gtcccgccgc gcgacatgcc
     1201 gcatcgagct cggccatctt cgccttcgcc gttgccgggg acgatgagct caggttgaac
     1261 tcgtccgcgt aggcggcggc gagccggaag ccacgcggtg tcccctgccc gcccgtgatg
     1321 atcctcggtc gtgccggccc ctcgcccgag ccaccgggac gcccgtgcgg tcggtcgggg
     1381 gcgtccacgg gccgcggatg cagcgcgccg ccgcggacct tgacgtggtg gccgtcgtag
     1441 tcccagccat cgggctcggt ccacaggccg aggagcagct cgagctgatc ctccatccag
     1501 tccgcccgcg cgcccatctc gggatacggg atgcccagcg gatcgtggtc ctcctggttc
     1561 cagccggcac ccacgccgac ctcgatgcgg cccccgctca tgtggtcgac ggtcgtcacg
     1621 agcttcacga agttgcccgg atggcggaac gtgaccgggc tgacgagcgc cccgagccgg
     1681 atcttcgacg tctcgcgaac gaggccggcc aggaccgccc aggtgtcggt ggagccccgg
     1741 ccggggtcac ccgggaaggc cgcgtagtgg tcgctgcgga acatcgcctc gaagcccgcc
     1801 gcctccgcgc gccgcgcaat ggccagctgg tcgtcgtacg agaggcccat ctgggcctcg
     1861 atcatcagcg cgaagcgcat cgggctactc ctcgtcgagc gccatcgcgg gcaaaccaac
     1921 ggcgacgaca cttgatgttg acgaaatcat tcatgtggat agaatgcttc acgtcatgcg
     1981 aagtatcgtc cagccaggaa ccgcggtccc cgcaggcgac agcgccgacg tcgaaaccat
     2041 cctgacagaa gtcagcgggt gggtccgcga gctgcgctgc gcgtcgatgg gccggatggt
     2101 ccagggcaag gtgagcatga gcaacatgca cgtcctgtgg ctgctccagc accagggcga
     2161 gatgccgatg agccggctcg ccgacctcct cgacgtgtcg ctctcgaatg ccacgggcat
     2221 cgtcgatcgc atggaggagc gtggcctcgt cgagcgagtc cgcgtcgccg atgaccggcg
     2281 catcgtcctc gtccgcccgg cagcggccgg gatgctcgcc ctctccgaaa cggaaacgac
     2341 caagcgcgaa cgcatgcgcg ccgtcgtccg ccggctcccc gccaccgagc ggcccgtggt
     2401 cctggctgcc ctccgcagcc tgcgccgcgc gctctcggcc gaggtcgaat cggcgccaac
     2461 acacgagcac cacttcgccg acgcggcgaa ctgacccgcc cccaataccc agcgaaggga
     2521 agtcgaccac atggaatcgt tcacgcccga aggccccgag acgccgtcga tcgccgagga
     2581 tccggcgctc ggactgagcc gccgcaccaa gttcgagatc ctcggcgcgg tgctgctcgg
     2641 catgttcctc tcggccctcg accagaccat cgtcggcccg gtcctgccga aaatcgttgg
     2701 cgagctcaac ggcagcgatt actacacctg ggccgtcacg atctacctgc tcacctcgac
     2761 cgtcaccgtg ccgatctacg gcaagctctc cgacctgtac ggccgcaagc cggtcctgat
     2821 cgccggaatc acgttgttcc tcgccggctc ggccttgtcc ggcctgagcc aggagatgtg
     2881 gcagctcgtg ctgttccggg gcgtccaggg cctcggcgcc ggcgcgctct tcccgatcgc
     2941 cctggcggtc atcggcgacc tcttcacccc cgccgagcgc ggcaagtacc agggcctgtt
     3001 cggcctcgtg ttcggtgtgg cgttcctgct tgggccggcc atcggcggca cgctgaccga
     3061 caccttcagc tggcacgcca tcttctacgt gaacctgccg atcggcgcca tcgcgctgtt
     3121 cgtgatctgg cggctcctgc cgaacatcaa gcacacggag cgcgccggca agatcgacta
     3181 cctcggtgtg gtcaccctga cgctcgggct cgttcccctg ctcatcggct tcaccgtcgc
     3241 cgagacgacg ggcttcggcg atccgttctt ctggctgtgg gccggcgcgg gcctcgtctt
     3301 cctggcgatc ttcgtcatgg tcgagcggcg cagccccgag ccggtgatcc cgcttcacct
     3361 gttccggaac cgcacgttct cgtcgtcgat gctgtcgatc ttcttcgcga cgttcggctt
     3421 cggcgccgtg atcatcttcc tgccgctgta cttcctcatc gtcgagggcg cgaccgcgac
     3481 cgagtcgggc taccggctcc tgccgttcat gttcggcctc atcctggcct cgatcgcgtc
     3541 cggccagatc gtgagccgca ccggccgcta caagccggtc gtcctcgtcg ggctcgtgac
     3601 cttgatcgtc ggcatggcgc tcatgaccca gctccgggcc ggcacctcgg acctggtgct
     3661 cgccgcctgg atgttcgtcg ccggcctcgg cgtcggaccc accttcgccg tgttcacgat
     3721 catcgtccaa aacgcggtcc cgttccatga gctgggcacg gcgacgagcg acctcacgct
     3781 cttccgccag atcggcacga ccattggcat cgccgtcgcg ttcacgatct tccggctcaa
     3841 cttcacgtgg gacctgatca aggagcagct tggtgccgcg ggcgtccctg cggcgttcat
     3901 ccctgccgcg ccgccagccg gattcgatcc cggcgcgctg accaacccga gcggtggcgg
     3961 cgccgccttc gacttcatga agctggtccc cgaagaggtg cggccgctca tcctcgacgg
     4021 gatccaccgg gcgctgacga tctcgatcg
//
LOCUS       DATBSW010000062        12174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_216611, whole genome shotgun sequence.
ACCESSION   DATBSW010000062 DATBSW010000000
VERSION     DATBSW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216611"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..668
                     /locus_tag="VIF63_01640"
     CDS             168..668
                     /locus_tag="VIF63_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8238112.1"
                     /translation="MTDLSERAVASAVPGVIDEKLKSLSRREPLTVAPGTSLQACLEL
                     IRETGTADSVFVTDPKGRLLGVLTERDIFGRLVTPDADLSQPVERLMVDHPNTLHLDE
                     PIRRAIELMQTGKFRNVPLVDDDSVLVGVVRPVDVLKALAEAFPEELLNLPPRPHQLM
                     DATEGA"
     gene            665..2542
                     /locus_tag="VIF63_01645"
     CDS             665..2542
                     /locus_tag="VIF63_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY8238113.1"
                     /translation="MSTEAPPTIPPTTQKQLERVTIRFAGDSGDGMQLTGSQFTRTAA
                     AFGNDISTYPDFPAEIRAPAGSLPGVSGFQLSFSSTEIHTPGDEPDVLVAMNPAALKA
                     NLGELPAGGALIVNEDAFTAQNLTKVGYASNPLSDGSLKQWNVFNVPVSTLNSRALEG
                     LGLTNKQVDLTKNFFALGLMFWLYERDMAGTIKWIDDKFSKRPEIAEGNKRALKAGYA
                     FGETTEIFHERYIVPPAKLSPGHYRNITGNEATALGFVAASQLAGRDLFYGSYPITPA
                     SDILHQLAGYKNFGVKTFQAEDEIAAIGAAIGASYGGAMGMTASSGPGIALKQEALGL
                     AVMVELPLVVVDVQRAGPSTGMPTKNEQADLLQVMFGRNSDSPVAVVAPATPAECFTF
                     SIEAWRIALKYMTPVVYMSDAFLASGAEPWRIPDVADMPSIAVPNRTERQGFYPYLRD
                     ETTLARPWAVPGTPGLEHRIGGLEKADILGNVSYDPDNHHRMQLLRQAKIAGIAADIP
                     PLEVHGPAEGDLLVLGWGSTYGAIRSAVERLQGRGASVSHAHIRYLNPFPANTGDVVG
                     AFKRVLIPEVNLGQLSMLIRAKYLVDAIGYNRVRGKPFRIAELEAEALRVLATIGVTV
                     Q"
     gene            2548..3627
                     /locus_tag="VIF63_01650"
     CDS             2548..3627
                     /locus_tag="VIF63_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY8238114.1"
                     /translation="MKTNGAGNGAGAGTGAGAAAATAATELPVLTRKDFVSDQEVRWC
                     PGCGDYSILAQTQKVMPDFGYPKENIVFISGIGCSGRLPYYMNTYGFHTLHGRAPAIA
                     TGLKAARPDLMVWVITGDGDALSIGGNHLIHSLRRNVDVRIIMFNNRIYGLTKGQASP
                     TSEFGKVTKSSPYGTIDFPVSPLAIALAAEASFIARSVDTHTEHLQKTLEAAGTHKGS
                     TFVEVLQNCNIFNDGAFRDFTDREVREDRMLMLEHGQPMIFGKDRNQGIRLIGHQPTV
                     VTIGEDGVTEADLLVHDETDPLIAFMLAHLWWPDFPVPVGIVRNISRPTHDQMIGDQI
                     AQATAARGTGDLKKLLNTGETWTVE"
     gene            3760..4260
                     /locus_tag="VIF63_01655"
     CDS             3760..4260
                     /locus_tag="VIF63_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8238115.1"
                     /translation="MICPVCRFGNFPGDDTCANCGADLTSVDTPQGITTFRGRLLGLH
                     LDELGAPEPHLIDVAAPIDEAIGRMREEAVDCLLVTDGGKLVGIFTDRDAILKVAGQP
                     AGDRTIGDVMTHDPVILRSGDPVAVAIHKMAVGGFRHVPIVKGGVPVGVVSARDVFRH
                     LAAGLD"
     gene            4253..4642
                     /locus_tag="VIF63_01660"
     CDS             4253..4642
                     /locus_tag="VIF63_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238116.1"
                     /translation="MTEAADPPRADAPAQPPRRVLVLADDLIWATRLADAITAAGGSP
                     QRIRRLEDLDRDPAEFAIVDLTARAYDGLEAIRIARGTGARVVAVGQHDDVPLRKAAL
                     AAGAERVFTYRALFEDGPRVIAAWLAR"
     gene            4639..5781
                     /locus_tag="VIF63_01665"
     CDS             4639..5781
                     /locus_tag="VIF63_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY8238117.1"
                     /translation="MSESETAGQHRLDRAQAGLRAIGADALLIGIGADLQYLTGYSTH
                     ALERLTMLVLPAAGRPALIAPRLEAMAAATSPAAAAGLLDVEAWDETDDPYRLVADRL
                     AAPAEGTRLMVDAGLPARHLLAIQGVLPDALFGLATDVTRELRMVKGPQEVDALRRAA
                     HAADRVVAQIAAGRLVGRTEADVSREVRDRLVAEGHETASFAIVASGPNGASPHHAAS
                     ERVIQAGDPIVLDIGGTLDGYGSDITRMLWVTGGDPDSGPTPAFLEAFELVRAAQAAA
                     TAAVRPGVGCGELDEIARRIIHDGGHGNDFIHRLGHGIGLEAHEEPYLVAGNAERIAP
                     GHAFSIEPGIYLSGRFGVRIEDIVVCAADGPDVLNLAPHDLLVVDG"
     gene            6369..6734
                     /locus_tag="VIF63_01670"
     CDS             6369..6734
                     /locus_tag="VIF63_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238118.1"
                     /translation="MARPGPNGERPIQNGDATHATEGDDGSAERGATAPQLRRFIKSR
                     AYVPMHELRRRFAINGADDEVTPVDLERRRIFVGLPEREGRLLGELLRGGDIGFELSM
                     DPISPIVVGVYPMRPVTRS"
     gene            6904..7836
                     /locus_tag="VIF63_01675"
     CDS             6904..7836
                     /locus_tag="VIF63_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238119.1"
                     /translation="MIRKHLGRIATALTTGALLGAMVGAAPASAKTPGWQFLNVQQLP
                     ATVADGGVAGFSFRIFNNGKSNISALYLTDSVVAAPVYLTNSRGTVCQTTPDLRCSFG
                     ALRSKAFIDVTIAYRVGTTDFTNTFQLDTTGDPPGGNNSHGDSLKVTKTTVVNSSQEF
                     DGGFVVDSTALQTGGELGNTNKQTTKLVPPSSLIPATIEDGITTGIPCAIGACAHQVG
                     EWSKLNVNNGATYGAAFKVTLMVWGGSVPGEVGAADIFLLHTLDNGTTYVINTVCNSE
                     NAPTNAECIKVTKIGCNFRIVAWLFSNGGIRGGF"
     gene            complement(7833..11714)
                     /locus_tag="VIF63_01680"
     CDS             complement(7833..11714)
                     /locus_tag="VIF63_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8238120.1"
                     /translation="MDGQVCQTCGEENPVDARLCGMCGSSLIEDEAAGEVRREVTLVT
                     SDLKGSTALGERLDPEALREVLNRYFDVMRAVFESHGGTIEKIIGDAIVAVFGLPFRH
                     DDDPLRALEAAAESQRALATLNDELDSGYGVRLVVRTGIATGEVTFGAAEGGQHVLLG
                     PAVDTSTVMEQNAPPFEVLVHESTQSRVGDAAEFEAAPPASPKGSDELLTAYRLVSVH
                     ERAGEVEAVVPESLPGMRICPSCGEQSPDRMRYCNTCGASLNQAIARESRRTVTIVFA
                     MPKVHSLNGEAPGPETMRDVMSTYFEGMRAALERHGGTVEKFIGDAVMAVFGLPVRHE
                     DDAVRAIRAAADMQTALESLNPGFRTESGLELSNHIGVNSGEVIAGDASTAQRMVTGD
                     AVNTAARLEQAAGSGEVVLGDLTYRLARSAIEVEFMPPLVLKGKAEPVPAYRLMRMAT
                     PAAAEASHGTPFVGREAEMGRLSGALAESIDTRGARFVTVVGDAGVGKSRLIREFASA
                     ASEQARLVRGRCLPYGDGITFWPLAEVIREAAGVSVEDTPRVATRRIDRLLERAAVED
                     REAITERVAAAINLSAAQFPVAELMWGGRRFLETLAAERPLVMIVDDLHWAEATFLDF
                     LDQLLETVTGASVLVLGSARHEIAERHAEWSAAHDSMLIRLEPLNESDAGRIVEELLG
                     SLDDKVRARIATAAEGNPLYVEQIVSMLVETGAIERGIDGWVARAGSDQLQIPPTVQA
                     LVASRLDALRSEERAVVDPASVIGLSFPVDAVMELVDETVRATLDSDLQALETKQLMR
                     RLEAEDAIYRFGHQIIRDTAYGSLLKRARAALHERFVTWAERVNRERGRELEFEEILG
                     YHLEQAYQYRTSLGVVDDTARGVGEAAATKLSSSGRRALARGDLPAATSLLRRSTAVL
                     PETSIFRIELLVDLADGLLQQGAFEDCRTVLDEAGRISAELDDERLSERVRLLEASHA
                     MFIGGVGVANRSLEAVAGAVPVLEKAGDDAGLARAARLEMYAYVMLGKFKEGTGSAAR
                     IVEHARKSGEERLVTRSIGPITYILVHGPMAVPAALGQSREMLDGITGDRKTEAVVHG
                     ALAQLLAMDNQFEEARGHYRRGQEILGELGAGIDAHSTSIDSGQVELLAGDPTAAASE
                     LQRDYDALESLGETWLRSTVAAMLGVAKWELGDLNEADRYAGIARSIADDDDVLSQVL
                     WRATAARIQAVMGDITAAIEIAEAAVKIAAGTEEVGLRADALAALAFVQSRATWDKEA
                     RASLDEALALLNAKGDRARARLLAPALNVRG"
     gene            complement(11756..>12174)
                     /locus_tag="VIF63_01685"
     CDS             complement(11756..>12174)
                     /locus_tag="VIF63_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8238121.1"
                     /translation="TVARAAPGEAIVKVGDPGEAAFFVMSGKAVAGIPEQSGYRALSS
                     MGPGDFFGEIAALTGSARTANVIADDTTDLLEVPKASLQELLELPELNSLINGRMQER
                     LGRTSTTDLPRLARPDQASLRDLRRKRPSGAAGSKA"
BASE COUNT         1855 a         4470 c         4092 g         1757 t
ORIGIN      
        1 atgcgcgtcg gcaggtttgc ccatgcgatc gcgtggctcc ggcatcgcga tccgctggcc
       61 ggccaggaga aggccgactt cgacgtcgaa tacgcggtcg tgctccgtcg cgcgctcgcg
      121 ctgatccccg aaccggccgc ctgatccgaa cgcactacac ttccctcgtg accgatctca
      181 gtgagcgagc cgtcgcctcg gccgttcccg gcgtcatcga cgagaagctc aagagcctca
      241 gccggcgcga gccgctgacg gttgcacccg gcacgtcgct ccaggcgtgc ctcgagctga
      301 tccgcgagac gggcacggcc gattcggtgt tcgtcacgga ccccaagggc cgcctcctgg
      361 gcgtgctcac cgagcgagac atcttcggcc gcctcgtgac cccagacgcc gacctgtcgc
      421 aaccggtcga gcgcctgatg gtcgaccacc ccaacacgct gcacctcgac gagccgatcc
      481 ggcgcgcgat cgagctcatg cagaccggca agttccggaa cgtgccgctc gtcgacgatg
      541 acagcgtcct cgtcggcgtc gttcgcccgg tcgacgtgct caaggcgctg gccgaggcgt
      601 tcccggaaga gctgctgaat ctgccgcccc gcccacacca gctgatggac gcgaccgagg
      661 gcgcatgagc accgaagcac cgccgaccat cccgcccacg acccagaagc agctcgagcg
      721 cgtcacgatc cggttcgccg gcgattccgg cgacggcatg cagctcaccg gctcgcagtt
      781 cacgcgaacg gcggctgcgt tcggcaacga catcagcacg tacccggact tcccggccga
      841 gatccgggcg cccgccggga gcctgcccgg cgtctccggc ttccagctga gcttctcgtc
      901 caccgagatc cacaccccgg gcgacgagcc cgacgtcctc gtcgcgatga acccggcggc
      961 gctcaaggcg aacctcggcg agctgcccgc gggcggcgcc ctgatcgtga acgaagacgc
     1021 gttcacggcc cagaacctga ccaaggtcgg ctacgcgagc aaccccctct cggacggctc
     1081 gctgaagcag tggaacgtgt tcaacgtccc ggtctcgacg ctcaacagcc gggcgctcga
     1141 agggctgggc ctgacgaaca agcaggtcga cctgacgaag aacttcttcg ccctcgggct
     1201 catgttctgg ctgtacgaac gcgatatggc cgggacgatc aagtggatcg atgacaagtt
     1261 ctcgaagcga cccgagatcg ccgagggcaa caagcgcgcg ctgaaagcgg gctacgcgtt
     1321 cggcgagacg accgagatct tccacgagcg gtacatcgtc ccgccggcga agctctcgcc
     1381 cgggcactac cgcaacatca cgggcaacga ggccaccgcg ctcggcttcg tcgccgcgtc
     1441 ccagctggcc gggcgcgacc tgttctacgg ctcatacccg atcacgccgg cctcggacat
     1501 cctgcatcag ctcgcgggct acaagaactt cggggtcaag acgttccagg ccgaggacga
     1561 gatcgcggcc atcggcgccg cgatcggggc gagctacggc ggcgcgatgg gcatgaccgc
     1621 atcgtccggc ccgggcatcg cgctcaagca ggaggccctc gggctcgcgg tcatggtcga
     1681 gctgccgctc gtcgtcgtcg acgtccagcg ggccggcccg tccaccggca tgccgaccaa
     1741 gaacgagcag gccgacctcc tccaggtcat gttcgggcgc aactcggact cgcccgtcgc
     1801 ggtcgtcgcg cccgcgaccc ccgccgagtg cttcaccttc tcgatcgagg cgtggcggat
     1861 tgcgctcaag tacatgacgc cggtcgtgta catgtcggac gcgttcctcg cgagcggcgc
     1921 cgagccgtgg cgcatccccg acgtcgcgga catgccgtcg atcgcggtcc cgaaccgcac
     1981 cgagcgccag ggcttctacc cgtacctgcg cgacgagacg acgctcgctc gcccgtgggc
     2041 cgtgccgggc acgcccggtc tcgagcaccg catcggcggc ctcgagaagg ccgacatcct
     2101 cggcaacgtc agctacgacc cggacaacca ccaccgcatg cagctcctgc gccaggccaa
     2161 gatcgccggc atcgccgcgg acatcccgcc gctcgaggtc cacggcccgg ccgagggcga
     2221 cctcctcgtg ctcggctggg gctcgacgta cggggcaatc cgcagcgcgg tcgagcgcct
     2281 ccagggtcgc ggcgcgtccg tgtcgcacgc ccatatccgc tacctgaacc cgttcccggc
     2341 gaacaccggc gacgtggtcg gcgccttcaa gcgcgtgctc attcccgagg tgaacctggg
     2401 ccagctgtcg atgctcatcc gggcgaagta cctggtcgac gccatcggct acaaccgggt
     2461 ccgcggcaag ccgttccgga tcgccgagct cgaggccgaa gcgctgcggg tcctcgccac
     2521 catcggggtg accgtccaat gaccgacgtg aagacgaacg gcgctgggaa cggcgcgggc
     2581 gcgggcaccg gtgcgggcgc ggctgccgcg actgccgcga ccgagctgcc ggtgctgacc
     2641 cggaaggact tcgtgtccga ccaggaggtc cgctggtgcc cgggctgcgg cgactactcg
     2701 atcctcgccc agacccagaa ggtgatgccc gacttcggct acccgaagga gaacatcgtc
     2761 ttcatctccg gcatcggctg ctccggccgg ctgccgtact acatgaacac ctacggcttc
     2821 cacaccctcc acggccgcgc gcccgcgatc gcgacgggcc tcaaggccgc gcggccggac
     2881 ctcatggtct gggtgatcac gggcgacggc gacgcgctgt cgatcggtgg caaccacctg
     2941 atccactcgc tgcggcgcaa cgtcgacgtc cgaatcatca tgttcaacaa tcggatctac
     3001 ggcctgacca agggccaggc gagcccgacc tccgagttcg gcaaggtcac caagtcctcg
     3061 ccgtacggca cgatcgactt cccggtgagc ccgctcgcga tcgccctcgc ggccgaggcg
     3121 agcttcatcg cccgctccgt cgatacccac accgagcacc tccagaagac tctcgaggcg
     3181 gccgggaccc acaagggctc gacgttcgtc gaggtcctcc agaactgcaa catcttcaac
     3241 gacggcgcat tccgcgactt caccgaccgc gaggtccggg aggaccggat gctcatgctc
     3301 gagcacggcc agccgatgat cttcggcaag gatcggaacc aggggatccg gctgatcggc
     3361 catcagccga cagtcgtcac gatcggcgag gacggtgtca ccgaggcaga cctgctcgtc
     3421 cacgacgaga ccgacccgct gatcgcgttc atgctcgccc acctgtggtg gcccgatttc
     3481 ccggtgccgg tggggatcgt gcgcaacatc tcgcgcccga cccacgacca gatgatcggc
     3541 gaccagatcg cccaggcgac cgccgcgcgc ggtaccggtg atctcaagaa gctgctgaac
     3601 accggcgaaa cctggacggt ggagtgacgc ggtgatcggg ccccatcgcc gcgttggctg
     3661 ctgcgcgtcc tcgctcacgc acatccacag tgcgctcgcg aggcctgctc gcagccgcct
     3721 tgcgctggga cccgctgacg gcgccactcg ggacctggcg tgatctgccc cgtttgccgc
     3781 ttcgggaact ttcctggcga tgacacctgc gcgaactgcg gggccgacct tacgagcgtc
     3841 gacacgcccc agggcatcac gaccttccgc ggccgcctgc tcggcctgca cctcgacgag
     3901 ctcggcgcgc ccgagccgca cctgatcgac gtggcggcgc ccatcgatga agcgatcggc
     3961 cggatgcgag aggaggccgt tgactgcctg ctcgtgacag acggcggcaa gctcgtgggc
     4021 atcttcaccg accgtgacgc gatcctgaag gtcgcggggc agccggccgg cgatcgaacc
     4081 atcggcgacg tgatgacgca tgacccggtg atcctgcgca gcggcgaccc tgtcgcggtg
     4141 gcgatccaca agatggccgt cggcggcttc cgacacgtgc cgatcgtcaa gggtggcgtt
     4201 cccgtcgggg tggtctcggc gcgagacgtg ttccggcacc tcgccgccgg gcttgactga
     4261 ggcggccgat ccgcctcgcg ccgacgcgcc ggcacagccg ccgcggcgcg tcctcgtcct
     4321 cgccgacgac ctgatctggg ccactcgcct cgccgatgcg atcacggccg ccggtggcag
     4381 cccccagcga atccgtcggc tggaggatct tgaccgcgac cctgccgagt tcgcgatcgt
     4441 cgacctgacc gcccgggcgt acgacggcct cgaggccatc cggatcgcgc gcggcaccgg
     4501 agcgagggtc gtcgcggtcg ggcagcacga cgacgttcct ctgcgaaagg cagcgctcgc
     4561 ggcgggcgcc gagcgcgtgt tcacctaccg agcgctgttc gaggacggcc cgcgggtcat
     4621 cgcggcgtgg ctggcgcgat gagcgagtca gaaaccgccg gccagcatcg actcgaccgg
     4681 gcgcaggcgg gcctgcgcgc gatcggcgcc gacgccctgc tgatcggcat cggcgccgat
     4741 ctgcagtacc tcaccggcta ctcgacccac gccctcgaac ggctcacgat gctggtcctc
     4801 ccggcggccg ggcgacccgc cctcatcgcg ccgcgcctcg aggccatggc ggctgcgacc
     4861 agcccggccg ccgcggccgg cctgctcgac gtcgaagcat gggacgagac ggacgacccg
     4921 taccgcctcg tcgccgatcg actggcggcc cccgccgagg gcacccgcct catggtcgac
     4981 gcgggcctgc ccgcacgcca cctcctggcg atccagggcg tgttgccgga cgcgttgttc
     5041 ggcctcgcca ccgacgtcac ccgcgagctg cggatggtca agggcccaca ggaggtggat
     5101 gccctgcgac gcgccgccca cgccgccgat cgcgtcgtgg cgcagatcgc cgccggcagg
     5161 ctcgtcggca ggaccgaggc cgatgtcagt cgcgaggttc gcgaccggct cgttgccgag
     5221 ggccacgaaa cggcgtcatt cgcgatcgtc gcgtcgggtc ccaacggcgc gtcgccgcat
     5281 cacgcggcaa gcgaacgagt catccaggcc ggcgacccga tcgtcctcga tatcggcggg
     5341 acgctcgacg ggtacggctc ggacatcacc cgaatgctgt gggtcaccgg cggcgatccc
     5401 gattcagggc ctacgccggc cttcctcgag gccttcgagc tggttcgggc agcgcaggcc
     5461 gcggcgaccg cggcggtccg gccaggcgtc ggctgcggcg agctcgacga aatcgcccgc
     5521 aggatcattc atgacggcgg tcacggcaac gacttcatcc accgcctcgg ccatggcatc
     5581 gggctcgagg cgcacgagga gccgtacctc gtcgcgggca atgccgagcg aatcgcgccc
     5641 ggtcacgcct tcagcatcga gccgggcatc tacctgtccg gccggttcgg cgtccggatc
     5701 gaggacatcg tggtctgcgc tgcggacggc ccggatgtcc tcaaccttgc gccgcacgac
     5761 ctcctggtcg tcgacggcta gctccgcgac ccggggtatc atcccggcga cgggcatccg
     5821 cccccgcgac cctgcacagg cccggatatg cgttcgacct accagcggcg cctcggcgct
     5881 gcgcttctcg gccgatgagc cgacgctgcc atcacggctc gcctcgacac gccccactcg
     5941 ggcgcgtccc gtaccgtccc gcgccgctca cctcggagcg ccggccggcg ccgatcgagg
     6001 gcgaacgacc acgttcgacc acgaccccgc cgcctgcccg acccgcagcg cccgccggcg
     6061 gtgaacaaac cgcggtccac gcggtggcgc ccgccgagcg cttgacggac gcgccgccct
     6121 tcgcgcgcac gccgcccttc gcgcacgcgg agccgctggc cgacgtcgag cttgcggtgg
     6181 atcccgtcga gaccccagac gcgcccgccg aacgaacagc cgcggcaacg gcaccaatcg
     6241 cgacgccgcc acgagcacca acgccggatc caaaggcggc ggctcgcgcg accgtgccca
     6301 cgccgcccgg cccgcgaccg gcgccggtgg gccgcggccc gcacgcccca gccgcctacc
     6361 agggagcctt ggcgagaccg ggcccaaacg gcgagcgacc gatccagaac ggcgacgcga
     6421 cgcacgcgac cgagggtgac gatgggtcgg ccgagcgtgg cgccaccgcg ccgcaacttc
     6481 gccggttcat caagagtcgc gcctacgtgc cgatgcacga gctccgtcgc cggttcgcga
     6541 tcaatggcgc cgacgacgag gtgaccccgg tcgacctgga gcgccggcgc atcttcgtcg
     6601 gccttccgga gcgggagggc cgcctgcttg gcgagctgct gcgcggcggc gacatcggtt
     6661 tcgagctgtc gatggacccg atcagcccga tcgtcgtcgg cgtgtatccg atgcggccgg
     6721 tgactcgcag ctagcggtgc attccggcaa cggaggcgtc atggagcggg tcgcgaacct
     6781 tgaccgctga ctgtcaagtc tgcgacgctc cgggttcgga ctttcgtcca tactcactag
     6841 cacattcgta ctaccgcaag cagcagtgaa tcgtcgggac cagcccgacc ggaggagcct
     6901 cgagtgatcc gcaagcatct tggtcgaatc gcgaccgctc tcaccaccgg agccctcctc
     6961 ggggccatgg tcggcgccgc acctgcgagc gcgaagacgc cgggctggca gttcctcaac
     7021 gtccagcagc tgcccgcgac cgttgctgat ggcggcgtcg ccggcttctc gttccgcatt
     7081 ttcaacaacg gcaagtcgaa catctcggcg ctctacctga ccgactcggt cgtcgcggcg
     7141 ccggtgtacc tcacgaacag ccgcggcacg gtctgccaga cgacgcccga cctgcgctgc
     7201 tcgttcggcg ccctgcggtc gaaggccttc atcgacgtga ccatcgcgta tcgcgtcggc
     7261 acgacggact tcacgaacac cttccagctc gacacgacgg gtgacccccc gggtggcaac
     7321 aacagccacg gcgacagcct gaaggtcacg aagaccacgg tcgtcaactc gagtcaggaa
     7381 ttcgacggtg gcttcgtcgt cgactcgacg gccctccaga ccggcgggga actcggcaac
     7441 accaacaagc agacgaccaa gctcgtcccg ccctcgtcgc tcatccccgc gacgatcgag
     7501 gacggcatca cgaccgggat tccgtgcgcg atcggcgcgt gcgcccacca ggtcggggag
     7561 tggtccaagc tcaacgtcaa caacggggcg acctacggcg ccgcattcaa ggtcaccctc
     7621 atggtctggg gcggatcggt gcccggcgag gtcggtgccg cggacatctt cctcctccac
     7681 accctcgaca acggcacgac ctacgtcatc aacacggtgt gcaactcgga gaacgcgccg
     7741 accaacgccg agtgcatcaa ggtcaccaag atcggttgca acttccgcat cgtcgcctgg
     7801 ctcttcagca acggcgggat ccgcggcggg ttctagcccc gtacgttgag tgcgggcgcg
     7861 agcaatcgcg cccgggctcg atctcccttc gcgttcagga gcgcgagcgc ctcgtcaaga
     7921 gacgctcgcg cttccttgtc ccaggtcgcg cgtgactgca cgaatgccag cgccgcgagg
     7981 gcgtcggccc ggagcccgac ttcctcggtt cccgcggcga tcttgacggc cgcctcggcg
     8041 atctcgatcg cggcggtgat atcgcccatc accgcctgga tcctcgccgc ggtcgcccgc
     8101 cacagcacct gcgagaggac atcgtcgtcg tcggcgatgc tcctggcgat cccggcgtag
     8161 cggtcggcct cgttgaggtc gccgagctcc catttcgcca cgccgagcat cgccgcgacc
     8221 gtcgagcgca gccacgtctc cccgagcgat tcgagcgcgt cgtagtcgcg ctgcagctcg
     8281 gacgccgcgg ccgtgggatc gcccgccagg agctcgacct ggccggaatc gatcgatgtg
     8341 gaatgggcgt cgatgcccgc gcccagctcg ccgagaatct cctggccgcg ccggtagtgc
     8401 ccgcgcgcct cctcgaactg gttgtccatc gcgagcagct gggcgagggc gccgtggacg
     8461 acggcctcgg tcttgcggtc gccggtgatg ccatcgagca tctcgcggct ctggccgagc
     8521 gctgctggga ccgccatcgg accgtggacg aggatgtacg tgatcgggcc gatgctgcga
     8581 gtgacgaggc gctcctcgcc tgacttccgg gcgtgctcga cgattcgcgc cgcggatccc
     8641 gtgccctcct tgaacttgcc gagcatcacg tacgcgtaca tctcgaggcg cgcggcgcgg
     8701 gccaggccgg cgtcgtcgcc ggccttctcg aggaccggca cggcaccggc gacggcttcg
     8761 agcgagcggt tcgccacgcc gacgccgccg atgaacatgg cgtggctcgc ctcgagcagc
     8821 cggacccgct cactcagtcg ctcgtcgtcc agctcggccg agatgcggcc ggcctcgtcg
     8881 agcacggttc ggcagtcctc gaacgcgccc tgctgcagga ggccgtcggc gaggtcgacg
     8941 agcagctcga tccggaagat ggaggtctcg ggcaggaccg ccgtcgagcg gcggaggagg
     9001 ctcgtcgcag cgggcaggtc gccccgggcg agcgcgcgcc ggccggagga ggagagcttc
     9061 gtcgcggccg cttcgccgac cccgcgcgcc gtgtcatcca cgacgccgag gctcgtgcgg
     9121 tactggtagg cctgctcgag gtggtagccg aggatctcct cgaactccag ctcgcggccg
     9181 cgctcacggt tgacccgctc ggcccaggtc acgaatcgct cgtgcagggc ggcgcgggcg
     9241 cgcttgagga ggctgccgta tgccgtgtcg cgaatgatct ggtgaccgaa ccggtagatc
     9301 gcatcttcgg cttccaggcg gcgcatcagc tgcttcgtct cgagggcctg gaggtcgctg
     9361 tcgagcgtcg cccgcacggt ctcgtcgacc agctccatca cggcgtcgac cgggaacgac
     9421 aggccgatga ccgaggccgg gtcgacgacc gcacgctcct ccgacctgag cgcatcgagg
     9481 cgtgaggcga ccagggcttg caccgtcggc gggatctgga gctggtccga accggcacgg
     9541 gcgacccagc cgtcgatgcc gcgctcgatc gcgccggtct ccacgagcat cgacacgatc
     9601 tgctcgacgt acagcgggtt gccctccgcg gccgtcgcga ttcgcgctcg gaccttgtcg
     9661 tcgagggagc ccaggagctc ctcgacgatg cgcccggcgt ccgactcgtt cagcggctcg
     9721 agccggatca gcatcgaatc gtgggccgcg gaccactcgg catggcgttc ggcgatctcg
     9781 tggcgcgccg acccgagcac gagcaccgat gcgccagtca ccgtctcgag cagctggtcc
     9841 aggaagtcga ggaacgtcgc ctccgcccag tgcaggtcgt cgacgatcat gaccagcggg
     9901 cgctccgcgg cgagggtctc gaggaaccgc cggccgcccc acatgagctc ggcgaccggg
     9961 aactgcgcgg cactcaggtt gatcgcggcc gcgacgcgct cggtgatggc ctcacggtct
    10021 tccaccgcag ctcgctcgag caggcgatcg atgcgccggg tcgcgacgcg cggggtgtcc
    10081 tcgaccgaga cgccggcggc ttcgcgaatc acctcggcga gaggccagaa cgtgatgccg
    10141 tcgccgtacg gcaggcagcg tccgcgaacc aggcgcgcct gctcggacgc cgccgaggcg
    10201 aactcgcgga tcaggcgcga cttgcccacg ccggcgtcgc cgacgaccgt cacgaagcgg
    10261 gcgccgcggg tgtcgatgga ttcggccagg gcgccggaaa ggcggcccat ctcggcttcg
    10321 cggccgacga acggcgtgcc gtggcttgcc tcggccgcgg ccggcgtggc catgcgcatc
    10381 agccggtagg cgggcaccgg ctcggccttg cccttgagca cgagcggggg catgaactcg
    10441 acctcgatcg cggagcgagc cagccggtag gtcaggtcgc cgaggacgac ctcgccactg
    10501 cccgccgcct gctcgagtcg cgccgccgtg ttcaccgcgt cgccggtgac catgcgctgg
    10561 gcggtcgacg catcgccggc gatcacctcg ccgctgttga cgccgatgtg gttgctgagc
    10621 tcgaggccgg actcggtgcg gaaccccggg ttcaggctct cgagcgcggt ctgcatgtcg
    10681 gcggcggcgc gaatcgcgcg gaccgcgtcg tcctcgtgcc gcaccggcag gccgaacacc
    10741 gccatcaccg cgtcgccgat gaacttctcg acggtgccgc cgtggcgctc gagggcggcg
    10801 cgcatgccct cgaagtacgt cgacatcacg tcgcgcatcg tctccggccc gggcgcctcg
    10861 ccgttcagcg agtggacctt cggcatcgcg aacacgatcg tcaccgtgcg ccggctctcg
    10921 cgcgcgatcg cctggttgag cgacgcgccg cacgtgttgc agtagcgcat ccggtcgggc
    10981 gactgctcgc cgcagctcgg acagatgcgc atcccgggca gggattcggg gacgaccgcc
    11041 tcgacctcgc cggcccgttc gtgcacggac acgaggcggt acgcggtgag cagctcgtcg
    11101 gagcccttcg ggctggcggg cggcgcagcc tcgaactccg ccgcgtcacc gacgcgggac
    11161 tgcgtcgact cgtgaacaag gacctcgaac ggcggtgcgt tctgctccat gaccgtcgag
    11221 gtgtccaccg ccggcccgag aagcacgtgc tgcccgccct cggccgcccc gaacgtgacc
    11281 tcgcccgtcg caatcccggt gcgcacgacg agccggacgc cgtagcccga atcgagctcg
    11341 tcgttgagcg tggcaagggc gcgctgcgac tcggccgccg cctcgagcgc gcgcagggga
    11401 tcgtcgtcgt gccggaacgg cagcccgaag accgccacga tcgcgtcgcc gatgatcttc
    11461 tcgatcgtcc cgccgtgcga ttcgaacacg gcgcgcatga cgtcgaagta gcggttcagg
    11521 acctcgcgca gcgcctcggg atcgagccgc tcgcccaggg cggtcgagcc cttgaggtcg
    11581 gaggtgacga gggtgacctc gcgccggacc tcgcccgcgg cctcgtcctc gatgaggctg
    11641 ctgccgcaca tcccgcacag gcgggcatcg accgggttct cctcgccgca cgtctggcag
    11701 acctgcccat ccaccaaggc agtctagcgg cggcccccgc cggctcagcc aaaggtcagg
    11761 ccttgctccc ggcggcgcca ctcgggcgct ttcggcgcag gtcgcgcagc gaggcctggt
    11821 cgggtcgggc aaggcgcggg aggtcggtcg tcgacgttcg cccgagccgc tcctgcatcc
    11881 ggccgttgat gagcgagttg agctcgggga gctcgagcag ctcctgcagc gatgccttgg
    11941 gcacctcgag gagatcggtc gtgtcgtcgg cgatgacgtt ggccgtgcgc gcgctgccgg
    12001 tcagcgccgc gatctcgccg aagaagtcgc ccggacccat cgacgacagg gcgcggtagc
    12061 cgctctgctc cgggatgccc gcgaccgcct ttccggacat gacgaagaac gcggcctcgc
    12121 cgggatcgcc gaccttcacg atcgcctcgc cgggcgccgc gcgggcgacg gtga
//
LOCUS       DATBSW010000063         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_745349, whole genome shotgun sequence.
ACCESSION   DATBSW010000063 DATBSW010000000
VERSION     DATBSW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745349"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1881
                     /gene="pckA"
                     /locus_tag="VIF63_01690"
     CDS             229..1881
                     /gene="pckA"
                     /locus_tag="VIF63_01690"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY8238122.1"
                     /translation="MTATTPTHPPSTRAPHGATRVRAGKIVANLSTAALYEAAVREGE
                     GLIAAEGPLVVSTGKHTGRSPKDKFIVREPSSEANIWWGDVNHEISEAHYDRLRARLM
                     TYLADRRVYSQDMFIGAHPAHRRSLRVYTETAWASIFARNLFRRPAPADMAGFAPNFT
                     IIDVPSFQADPATEGVRSGTAILVHLQRMEIIIVGTMYAGEIKKSAFTVMNYLMPDEG
                     VLPMHSSINVGVDGDSAVFFGLSGTGKTTLSADPLRSLIGDDEHGWGSDYVFNFEGGC
                     YAKTIRLSPAYEPDIFAATRRFGTILENVDIDPATRELDLDSEKFTENTRGAYPLNFI
                     GNADPTGVAGTPRSVVFLTADAFGVLPPISRLTREQAAYHFISGYTAKLAGTEVGVKE
                     PTATFSAGFGAPFLPRHPGVYAELLMDRLDRFNVPVWLVNTGWTGGPYGIGERMNITH
                     TRNMVRAALGGQLRDVPMVIDPVFRVAVPTEVPGVPSEVLQPRSTWPDGAAYDVAARK
                     IAHMFHENFEAYAAGVSKAVREAGPIRADGVDDVPVTGPGEG"
     gene            complement(1888..>2079)
                     /locus_tag="VIF63_01695"
     CDS             complement(1888..>2079)
                     /locus_tag="VIF63_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238123.1"
                     /translation="AQIQCAGGKNNMNLNIPVVALSIVGVLIAAAGIFIDQSIFLIAA
                     GVGTILFAWVLQEMSKRRA"
BASE COUNT          357 a          743 c          669 g          310 t
ORIGIN      
        1 ctcgaccgcg tgcggcccct gtgggcgacc ttcgagcgag cgctcgccgg ctgagcaggg
       61 aggaccttcg acccatcccg ccacagcgag ccggtggtag catgccgctc aactacgact
      121 gcggaagccg actcccatcg agcttgctcg atgtcacgcg cggccggtcg ggatcgaagc
      181 acggcgcccg gactccccgg gactgcccga gcaggagacc gacccggaat gaccgcaaca
      241 accccgaccc acccgccgag tacacgcgcg ccgcatggcg caacccgtgt ccgggccggc
      301 aagatcgtcg ccaacctctc cacggccgcc ctgtacgagg ccgccgttcg cgaaggcgaa
      361 ggcctgatcg ccgccgaagg cccgctcgtg gtgagcacgg gcaagcacac cggccgctcg
      421 ccgaaggaca agttcattgt tcgcgagccg tcgagcgagg cgaacatctg gtggggcgac
      481 gtcaaccacg agatctccga ggcgcactac gaccgactgc gcgcgcggct gatgacgtat
      541 ctcgccgatc ggcgcgtgta cagccaggac atgttcatcg gcgcgcatcc ggcgcaccgt
      601 cgatcgctcc gcgtctacac cgagaccgct tgggcgagca tctttgcccg caacctcttc
      661 cgccggcccg cgccggcgga catggccggc ttcgcgccga acttcacgat catcgacgtg
      721 ccgtcgttcc aggcggaccc cgcgactgag ggcgtcaggt cgggcacggc gatcctcgtc
      781 cacctccagc ggatggagat catcatcgtc ggcaccatgt acgccggcga gatcaagaag
      841 tccgcgttca cggtcatgaa ctacctcatg cccgacgaag gcgtcctgcc gatgcactcg
      901 tccatcaacg tcggggtcga cggcgactcg gcggtgttct ttggcctgtc ggggacgggc
      961 aagacgaccc tgtcggccga tcccctgcgg agcctcatcg gcgatgacga gcatggctgg
     1021 ggcagcgact acgtcttcaa cttcgagggc ggttgctacg ccaagacgat ccggctctcg
     1081 cccgcctatg agcccgacat cttcgccgcg acgcgccggt tcgggacgat cctcgagaac
     1141 gtcgacatcg accccgcgac gcgcgagctg gacctcgact ccgagaagtt caccgagaac
     1201 acgcgcgggg catacccgct caacttcatc ggcaacgcgg atccgaccgg ggtcgcgggc
     1261 acgccccgat cggtcgtgtt cctgaccgcc gacgcgttcg gcgtgctgcc gccgatctcg
     1321 cgcctcaccc gcgaacaagc ggcgtaccac ttcatcagcg gctacacggc caagctcgcc
     1381 ggcaccgagg tcggggtgaa ggagccgacg gcgacgttct ccgcgggctt cggggcgccg
     1441 ttcctgccgc gccatcccgg cgtctatgcc gagctgctga tggatcgcct cgatcgattc
     1501 aatgtccccg tctggctcgt caacacgggc tggacaggcg ggccctacgg aatcggcgag
     1561 cggatgaaca tcacccatac ccggaacatg gtccgggccg ccctcggcgg ccagctgcgc
     1621 gacgtgccga tggtcatcga cccggtgttc cgcgtcgccg tcccgaccga ggtgcccggc
     1681 gtcccttcgg aggtcctcca gccacgctcg acgtggcccg acggcgccgc gtacgacgtc
     1741 gccgcgcgca agatcgcgca catgttccac gagaacttcg aggcgtacgc cgccggcgtg
     1801 agcaaggccg tacgcgaggc cggaccgatc cgcgccgacg gggtcgacga cgtgccggtg
     1861 accgggcccg gcgaaggctg atccgcgtca ggcccggcgc ttggacatct cctgaaggac
     1921 ccaagcgaac agaatcgtcc caaccccggc ggcgatgagg aagatgctct gatcaatgaa
     1981 gatgccggcg gcggcgatca ggactccgac gatcgacaga gcgaccacag gaatgttcag
     2041 gttcatgttg ttcttccctc cggcgcactg gatttgagc
//
LOCUS       DATBSW010000064         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_428562, whole genome shotgun sequence.
ACCESSION   DATBSW010000064 DATBSW010000000
VERSION     DATBSW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428562"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..399
                     /locus_tag="VIF63_01700"
     CDS             13..399
                     /locus_tag="VIF63_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEY8238124.1"
                     /translation="MPPIDELIRIEPIYLDRLQRQGIFTTGLLLEVTETPTRRQTLAD
                     QVDASTNDVLEWRDEAQMLNLAGFGPAEHRLLSQAGFEGLKDILLVDLDAFGKRIARA
                     ADELKMEPPTDLTIEGWWDQAHTLAE"
     gene            399..1112
                     /locus_tag="VIF63_01705"
     CDS             399..1112
                     /locus_tag="VIF63_01705"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HEY8238125.1"
                     /translation="MGTRLGGWLIAAAAALVILGASVAPFLTPPVVRFEQDRTGAAAL
                     TGYSPVQLDIVTGALLGDLVFGLGDFDVEEFESDGSIYPRILDDSERAHMRDVRTVFT
                     GFWLLVLGSIAVLAVAFRRGKDHRMRSAAWRAVSNGARALAIVIAILGAFALVAFDTA
                     FELFHRLFFSAGSYTFDPATSRLVQLFPDQFWSEITIAVGVVAITVALLTAWIAGRRA
                     ARGAAARETPILTATRAGA"
     gene            1112..2269
                     /locus_tag="VIF63_01710"
     CDS             1112..2269
                     /locus_tag="VIF63_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8238126.1"
                     /translation="MGIPIARIFGIEIRVTYGWVVVLAFVSVIAFGQLTSVDPALEEL
                     VAWGLGGLVALGFFVSSVSHDLAHAVVARRRGVEVRSIGISFFGGATPNDPASTNARD
                     DAVIAASGPIVSLALGGLLLGLMAAATSAGSGFTLAAGMFATLGFLNLVLGFVNLIPA
                     YPLDGGRLVRDIAWRWTGSERTGWRVAAQAGRLTGLVVIGIGVTYLLGSLNTTGLDAT
                     GAMIAITGWFLVLSGNAVRDRVRLDDMIGGHVVSDAMDADPTTVTPTLTVDTFAAQLL
                     DGETPVYAVPVVEGTEVVGLLGVNQVRRIRRTEWANTRVVDVMARPPKLVFLSPTEPL
                     KQGLERVYKAGLDGLPVMDDGRMVGILTKRGVGRFIGEKTLRLKGATPPVA"
BASE COUNT          308 a          789 c          801 g          389 t
ORIGIN      
        1 tatcatcgcc gcgtgccacc gatcgacgag ctgatccgca tcgagccgat ctacctcgac
       61 cgcctgcagc gccagggcat cttcacgacg ggcctcctcc tcgaggtcac ggagacgccg
      121 acgcggcgcc agaccctcgc cgaccaggtc gatgcgtcga cgaacgacgt cctcgagtgg
      181 cgcgacgagg cccagatgct caacctcgcg ggcttcgggc cggccgagca tcgactgctc
      241 agccaggccg gctttgaggg cctcaaggac atcctcctcg tcgacctcga cgcgttcggg
      301 aagcggatcg cgcgcgccgc cgacgagctc aagatggagc caccgaccga tctcacgatc
      361 gagggctggt gggaccaggc ccacaccctg gccgagtagt gggtactcgg ctcggtgggt
      421 ggctcattgc cgccgccgcg gcgctggtca tcctcggcgc ctcggtcgcc ccgttcctca
      481 cgccgcccgt cgtgcgattc gagcaggatc ggaccggcgc tgccgcgctg acgggctact
      541 cgcctgtgca gctggacatc gtcacgggag cgctcctcgg cgacctcgtg ttcgggctag
      601 gcgactttga tgttgaggag ttcgagagcg acggaagcat ttacccccga attctggacg
      661 actccgagcg ggcccatatg cgcgacgtgc gtaccgtctt cacggggttc tggctgctgg
      721 tcctcgggag cattgcagtg ctcgcggtcg cgtttcggcg ggggaaggac catcgcatga
      781 ggtccgcggc gtggcgggcg gtttcgaacg gggcgcgcgc gctggcaatt gtgatcgcca
      841 tcctcggtgc gttcgcactc gttgccttcg acacggcgtt cgagctgttc caccggctct
      901 tcttcagcgc cggcagctac acgttcgatc ccgcgacgag ccgcctcgtg cagctgtttc
      961 cggaccagtt ctggtcggag atcacgatcg cggtcggtgt tgtcgcgatc accgttgcgc
     1021 tcctcacggc gtggatcgcc ggccgacgcg cggcacgtgg cgccgctgcg cgcgagacgc
     1081 cgatcctcac cgcgactcgg gcaggcgcct gatgggcatc ccgatcgcgc gcatcttcgg
     1141 gatcgaaatc cgggtcacgt acggctgggt cgtcgtgctg gcgttcgttt cggtgatcgc
     1201 gttcggccag ctgacctcgg tcgacccggc gctcgaggag ctcgtggcgt gggggctcgg
     1261 cggcctcgtg gcgctcggct tcttcgtgag ctccgtttcc cacgacctcg cccacgcggt
     1321 cgtcgctcgc cggcgcgggg tcgaggttcg atcgatcggc atctcgttct tcggcggcgc
     1381 gaccccgaac gatccggcct ccacgaacgc gcgggatgac gcggtcatcg ccgcgtcggg
     1441 cccgatcgtg agcctcgcgc tcggtggcct gctcctcggg ctcatggcgg ccgcaaccag
     1501 cgcgggcagc ggattcacgc tggccgcagg gatgttcgcg acgctcgggt tcctcaacct
     1561 ggtgctcggc ttcgtcaacc tcatcccggc gtacccgctc gatggcgggc gcctcgtgcg
     1621 ggacatcgcg tggcgctgga ccggctcgga gcgcaccggc tggcgggtcg cggcgcaggc
     1681 cgggcggctc accggtctcg tcgtcatcgg catcggcgtg acgtacctcc tgggaagcct
     1741 caacacgacg gggctcgacg cgaccggcgc gatgatcgcg atcaccggct ggttcctcgt
     1801 gctgtcgggc aacgcggtcc gcgatcgggt gcggctcgac gacatgatcg gcggccacgt
     1861 cgtcagcgat gcgatggacg ccgatccgac gaccgtcacc ccgacgctca cggtcgacac
     1921 gttcgcagcc cagctgctcg acggcgagac gccggtctac gcggtgcccg tcgtcgaggg
     1981 caccgaggtc gttgggctgc tcggggtcaa ccaggtgcgg cgcatccgtc ggacggaatg
     2041 ggcgaacacc cgggtcgtcg acgtcatggc gaggccgccg aagctcgtct tcctcagccc
     2101 gaccgagccg ctcaagcaag gcctcgagcg cgtgtacaag gcggggctcg acgggctgcc
     2161 ggtgatggac gacgggcgaa tggtcgggat cctcaccaag cgcggcgtcg gccggttcat
     2221 cggcgagaag acgctgcggc tcaagggcgc gaccccgccc gtcgcgtgaa cgggctgatc
     2281 gccgcgt
//
LOCUS       DATBSW010000065         4638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_75656, whole genome shotgun sequence.
ACCESSION   DATBSW010000065 DATBSW010000000
VERSION     DATBSW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75656"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VIF63_01715"
     CDS             <1..792
                     /locus_tag="VIF63_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HEY8238127.1"
                     /translation="RGTVRPEVLGSYNVVLRISTDGGATWAYADRGGIVAQPDGTWGY
                     RADQAVTLAVTPNADAAAPPAAGNLRITTAGDDVVTLAWDAVAAPDLFRYEISRSSAA
                     GGPYELVGSSTDPTFTDASIRAGDSYWYVVATVDTAFNRSAPSTEVAAAAVSREVQVT
                     FTVTLPATTPAGDTIYIAGDFQGWNPAATPMTKVDDTTWTITVPFTEGIDPQYKYTRG
                     SWEAVEKDDGCAEIPNRTFSVAFGDTGAQPVADTVAKWRDVDQCG"
     gene            820..1422
                     /locus_tag="VIF63_01720"
     CDS             820..1422
                     /locus_tag="VIF63_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238128.1"
                     /translation="MSDLPVSLPSELAPAFVGAFDRAGKIVAALGTLGPVAGRDVLVV
                     DATGGPIVDGLGAAGARLTHLGIERPFRLEAPDGSADVVLGLWSAFRGVDWHERAEVD
                     RVLRPGGRHLAVHDYGRDDVSRLYADRPEYGEWSQRTGPFLTGGFRVRVVHCFWDFDD
                     AALAATFLSSAFGEAGATVAATLKRPRLSYNVAVYHRSRS"
     gene            1428..1787
                     /locus_tag="VIF63_01725"
     CDS             1428..1787
                     /locus_tag="VIF63_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238129.1"
                     /translation="MTTPASAPSPRRRGTWIGPVHLTFPLAVVLIVMTGSLIFIGWDV
                     RSNRDDQIPLLAVGFAALGASFAAIAVGALFNLWRAAARLRSRRAFGLALVGGLFGLA
                     AIGSFTVTALSMLVWNT"
     gene            1953..2029
                     /locus_tag="VIF63_01730"
     tRNA            1953..2029
                     /locus_tag="VIF63_01730"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1987..1989,aa:Glu,seq:ttc)
     gene            2692..3063
                     /locus_tag="VIF63_01735"
     CDS             2692..3063
                     /locus_tag="VIF63_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238130.1"
                     /translation="MSPLDYLGVHIPTIIEDNVSIRCDGCLEIIDGTPWRMNILDTVA
                     AETPVSWTGRPPINPGPFEFHGDGAHVRMWMAAKGYLFCRKGEVREIMRPLPIPGAEG
                     APLRYGLCDGLHRDDHELVPA"
     gene            3107..4183
                     /locus_tag="VIF63_01740"
     CDS             3107..4183
                     /locus_tag="VIF63_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238131.1"
                     /translation="MFTLLPALTAALALVMAIALIDQWRERHQPFQLVWAIGMTFYGI
                     AAGCEAIAAAAGWNEPLYRTWYLTGAVLTAGWLGLGTALLLAKTRFGYAIAVSIFLAG
                     LFALLTQRRFNYEGAGSTPILYFIGAGLLALAIGVETYFQNERWPMFMALGVGVASVL
                     ALVLAATVPLNQPFSIDPSTGQPSADLFDLSGAVAGTNLPGSLRLLTPFLNITGGFSL
                     AFGALFSAYVFMPKRRVLPYTLDPNQKGDHFLFNLLIAPVALTVNFFASLPGAVRDLA
                     LGRLHSRVPATLLIAAGGFVASAGDSLNRFGITGPFAVAKLVAAVLLLGGFLVSIEAF
                     REIRIPFTRIRLAGLRREAGPEPT"
     gene            4229..>4638
                     /locus_tag="VIF63_01745"
     CDS             4229..>4638
                     /locus_tag="VIF63_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8238132.1"
                     /translation="MPTPRAGWLGALLVIVAATLFGSLGVASRFAYDSGVQPFAFVAW
                     RAGVGALGLILVIAALRGPRAAWANIASASRSARLALALAVLLAAGLNLSMFLAFDRT
                     TVALALLGFYTYPALVAGASGFLGREPLDATRVVA"
BASE COUNT          582 a         1725 c         1531 g          800 t
ORIGIN      
        1 cgcggcaccg tgcggccgga ggtgctcggc tcgtacaacg tcgtcctgcg gatctcgacc
       61 gacggcggtg ccacgtgggc gtacgccgac cgcggcggga tcgtcgccca accggacggc
      121 acatggggct atcgagcaga ccaggccgtc acgctggccg tgaccccgaa cgcagacgcc
      181 gcggccccgc cggcggcggg caacctgcgg atcacgacgg ccggcgacga cgtggtgacg
      241 ctcgcctggg acgccgtggc cgcgcccgac ctgttccgct acgagatctc gcgctcgagc
      301 gcggcgggcg gcccgtacga gctcgtcggc agctccaccg acccgacctt caccgacgcc
      361 tcgatccggg ccggtgactc ctactggtac gtcgtcgcca cggtcgacac cgcgttcaac
      421 cgctccgcgc catcgacgga ggtcgcggca gcggccgttt cgcgcgaggt ccaggtcacg
      481 ttcacggtca ccttgccggc aaccaccccc gccggcgaca cgatctacat cgccggcgac
      541 ttccagggct ggaatccggc cgccacgccg atgaccaagg tcgacgacac cacgtggacg
      601 atcacggtgc cgttcaccga gggcatcgac ccgcagtaca agtacacccg cggctcgtgg
      661 gaggcggtcg agaaggacga cggctgcgcc gagatcccga accgaacgtt cagcgtggcg
      721 ttcggcgata ccggcgcgca gcccgtcgcc gacaccgttg cgaagtggcg cgacgtcgac
      781 cagtgcgggt gacctgacac tcgaacttat actcccgcga tgtccgatct cccggtgtcg
      841 ctgccgagtg agctcgcgcc ggccttcgtc ggcgccttcg accgtgccgg caagatcgtc
      901 gccgcgctcg ggacgttagg cccggtcgcc ggccgcgacg tgctcgtcgt cgacgccacc
      961 ggcgggccga tcgtcgatgg ccttggtgcc gcaggcgcgc ggctgaccca cctcgggatc
     1021 gagcgcccgt tccgcctcga ggcgcccgat ggctcggccg acgtggtcct cgggctgtgg
     1081 tcggcgttcc ggggcgttga ctggcatgag cgagccgagg tcgatcgcgt cctccggccc
     1141 ggcggccggc acctcgccgt ccacgattac gggcgcgatg acgtgtcgcg cctctacgcc
     1201 gaccggcccg agtacggcga gtggagccag cgcaccggcc cgttcctcac cggcgggttc
     1261 cgggtccggg tcgtgcactg cttctgggac ttcgacgacg cggcactggc tgcgacattc
     1321 ctttcgtcgg cgttcggcga ggccggggcc acggtcgccg caacattgaa acgccccagg
     1381 ctctcgtaca acgttgccgt ctaccatcga tcgcgatcgt gagtaacgtg accacgcctg
     1441 cgtctgcccc gtcaccccgg cgccggggta cctggatcgg cccggtccac ctgaccttcc
     1501 cgctcgctgt cgtgctgatc gtgatgaccg gctcgctgat cttcatcggc tgggacgtcc
     1561 gcagcaatcg tgacgaccag atcccgctgc tcgcggtcgg gtttgcggcc ctcggggcat
     1621 cgttcgcggc gatcgcggtc ggcgccctgt tcaacctgtg gcgcgcggcc gcccgactgc
     1681 gcagccgccg ggccttcggc ctggcgctcg tcggtggcct tttcggcctc gccgcgatcg
     1741 gctcgttcac cgtgaccgcg ctgtcaatgc tggtctggaa cacctgaagc tcgggctgat
     1801 ccggccccat cgctgcgttg tcggctgcgc ccgtcgttca cgcactcgag agtgcgctcg
     1861 ctccggtact cgcctccgcc ttgcgctgcg gccggctgcg cccgagctgg cgaggtccgc
     1921 ggggtcgctt gttacactcg cccggctctc gggcccccat cgtctagagg cctaggacgc
     1981 caccctttca aggtggagat cgccggttcg aatccggctg ggggtaccac cgcgctgggt
     2041 tgatccgggc ccatcgctgc gttgtcggct ccgcccgtcg ctcacgcact cgagtgtgcg
     2101 ctcgctctcg tgctcacctc cgccttgcgc tgcgcccggc tgaacccagc gcgaatacct
     2161 accaccgcgc tgggctgatc cgggcccatc gctgcgttgt cggctccgcc cgtcgctcac
     2221 gcactcgaga gtgcgctcgc tccgttgctc gcctccgcct tgccctggcc gcagcttggc
     2281 tcggcgctgg ctattcgcgg cccggctgac cgccgtgctc cgcgggcgct gatccggagc
     2341 ggatgccggc cgaccggctt cgatccaatc tcgacaacga cggccgaaag cggcctacgg
     2401 ccaccttcat gctccaagac gtccatcaga tcgcaccccg gacggctggg gcgaggaatc
     2461 cgcgtgcgca gcgcccatcc gatcgcccca aggcagccgt tcgaccgcgc ttgttgcgtt
     2521 ccaatcgcag gaggacggcg gaatttggcc ctgcgtgcgc gcggcactat cagggtgagc
     2581 ctggggcact cctccagccg gtcggcatgg ctcgcatcgt cctcgatgca cgcccgcctg
     2641 gcgtcgcgga ccggctcggc cgtttcgacg cgcactggct accatcgcgg cgtgagtccg
     2701 ctcgactatc tcggcgtgca cattccgacc atcatcgagg acaacgtctc gatccgatgc
     2761 gatggttgcc tcgagatcat cgatggcacg ccttggcgga tgaacatcct ggacaccgtt
     2821 gcggcggaga cgccggtctc gtggacgggg cggccgccga tcaaccccgg gccgttcgag
     2881 ttccatggcg acggcgccca cgtgcggatg tggatggccg cgaagggcta cctgttctgc
     2941 cggaagggcg aggttcgcga gatcatgcgc ccgttgccga tcccgggcgc cgagggggcg
     3001 ccccttcgct acggcctttg cgatgggctc caccgcgacg atcacgagct cgtccccgcc
     3061 tgacctcgcg caccggggcc ggcccagcac cgaaggatca cgatcgatgt tcaccctgct
     3121 gccagccctg accgccgctc tcgccctggt gatggcgatc gccctcatcg accaatggcg
     3181 cgaacggcac cagccattcc agctcgtctg ggcgatcggc atgacgttct acggcattgc
     3241 cgccggctgt gaggccatcg cggccgctgc cggctggaac gagccgctgt accgcacctg
     3301 gtacctgacc ggggcggtcc tcactgcggg ctggctcggc ctcgggacgg cgctgctgct
     3361 ggccaagacg cggttcggat acgcgatcgc ggtgagcatc ttcctcgccg gcctgttcgc
     3421 cctgctgacc cagcgccggt tcaactacga gggtgcgggc agcacgccga tcctgtactt
     3481 catcggggcc ggtttgctgg ccctcgccat cggcgtcgag acgtacttcc agaacgagcg
     3541 ctggccgatg ttcatggccc tcggcgtggg cgtcgcaagc gtgctcgccc tcgtgctcgc
     3601 ggcgaccgtc ccgctcaacc agccgttctc gatcgatccc tcgaccggcc agccgagcgc
     3661 ggacctgttc gacctctccg gagctgtcgc agggacgaac ctgcccggat cgctgcgcct
     3721 gctcacgccg ttcctcaaca tcacgggcgg cttctcgctc gcgttcggcg cgttgttctc
     3781 ggcgtacgtg ttcatgccca agcggcgcgt gctgccgtac acgctcgacc cgaaccagaa
     3841 gggcgaccac ttcctgttca acctgctcat cgccccggtc gcgctcaccg tcaacttctt
     3901 cgcctcgctg ccgggggcgg tgcgcgatct ggccctcggc cggctccaca gtcgcgtgcc
     3961 cgcgacgctc ctgatcgccg ccggcggctt cgtcgcgtcc gcgggcgact cgctgaaccg
     4021 cttcgggatc accggcccgt tcgccgtcgc caagctcgtg gccgccgtgc ttctcctggg
     4081 cggcttcctc gtctccatcg aggcgttccg cgagatccga atcccgttca cgcggatccg
     4141 attggccggg ctccgacgcg aggcaggccc cgagccgacc tgagccgacc tgagccaacc
     4201 tgaggcccgc gtgcgaggat tgccggccat gccaactccc cgcgccggct ggctcggcgc
     4261 cctcctcgtc atcgtcgctg cgaccctgtt cggctcgctc ggtgtcgcgt cgcggttcgc
     4321 gtacgactcg ggtgtgcagc cattcgcgtt cgtggcctgg cgcgcaggcg tgggcgcgct
     4381 cggcttgatc ctcgtgatcg ctgccctgcg cggcccgcgc gccgcgtggg cgaacatcgc
     4441 ctcggcgagt cgcagcgcgc ggctggcgct cgcgctagcg gtcctcctgg cggcaggcct
     4501 caacctctcg atgttcctcg cgttcgaccg aacgacggtc gcgttggccc tgctcggctt
     4561 ttacacctac ccggcgctgg tcgccggcgc gagcgggttc ctcggacggg agccgctcga
     4621 tgcgacacgg gtcgttgc
//
LOCUS       DATBSW010000066         8145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1028087, whole genome shotgun sequence.
ACCESSION   DATBSW010000066 DATBSW010000000
VERSION     DATBSW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028087"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VIF63_01750"
     CDS             <1..1060
                     /locus_tag="VIF63_01750"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY8238133.1"
                     /translation="PPASQTPSKDIGRPFEPLEECKTLGDYRMRYAQYRRDPDVQAVS
                     AAHPIISTVDDHEFADGAWRDGATEHKPERDGPWSERKAAAFRAREEWLPVRRPDPAN
                     AERVYRSVALGSPADLFMLDTRTMRDEPVPPPALSREGRTNLGADQKAWLLGALAASK
                     ARWRLLGNPSVMARTWNDALPEPARKALVKVKLIDTDGTGPDYDQWDGYPQERRELLR
                     AIGELDPPNVVVLSGDVHVGLAAELAEDPHMPPVAVEFVNTSLTSQNLDDKMGWAPRT
                     DSVVIEAALLAGMPHLHYVDLDSHGYSVVDLDRDRLRFEWWTVEQLVGRAPGQTLAAS
                     LEVRHGVAQLVGDAAVQP"
     gene            1088..2560
                     /gene="gatB"
                     /locus_tag="VIF63_01755"
     CDS             1088..2560
                     /gene="gatB"
                     /locus_tag="VIF63_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY8238134.1"
                     /translation="MHTSTATDRYEAVIGIEVHCQLRTASKMFCACSTAYDGARPNSH
                     VCPVCLGLPGALPVINRRAVELVLATGAAIGASTPDATRWDRKNYFYPDLPKGYQISQ
                     YDLPLAAAGTLTFETSDGPFTTRITRAHLEEDTAKLVHGEGEAGERTSLVDFNRSGMP
                     LMEIVTEPDLRTAEQARRYAEELQLLLRTIGASDADMERGQLRVEANVSLRPRGTEAF
                     GTRVEVKNMNSFRSVERAVEFEIERQAAALDAGEALRMETRGWSDDRGETYHMRNKET
                     SDDYRYFPEPDLPPLRVDAGWLAQIRGELPELPAAQRARYRQVLGLSVYDAALLTADP
                     DAGRLFEATLVAGGDVGAKPVANWVTGDYLRMRNAASAPITVDPAALADLIRRVVNGS
                     LNRSSAREVLDAHVATGETIEAIVARLGLAQISDAGALGSIVDDVLAANPAAVADIRA
                     GKPQAIGFLVGQVMKATRGQANSQVASAAIRARLGIDEPG"
     gene            2560..2823
                     /locus_tag="VIF63_01760"
     CDS             2560..2823
                     /locus_tag="VIF63_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238135.1"
                     /translation="MGLVLILAGIAATAAGYARFRVPWAKYQALKLQEANIARYESWR
                     GGLRDTGPTGASVAMEMLRKQARDSAVIMAAGIAVVILGFFVS"
     gene            complement(2816..3700)
                     /locus_tag="VIF63_01765"
     CDS             complement(2816..3700)
                     /locus_tag="VIF63_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238136.1"
                     /translation="MSGSVAGPSGGGPAAGLDVAVEGLSYRYRDGTQALTNVSLTVGV
                     GEAVALIGQNGSGKSTLVRHLNGLLRPTAGRVLVGGRDVADRRVAELARLVGLAFQNP
                     DRQIFAQTARAEVGFGARNIGRRGEALDAAVARALDAVGMAGLEEAHPYDLGYSKRKL
                     LTIASVLAMETPVLILDEPTTGQDAGGVLRIQRLVAELIADGRTVIAISHDIRFAAET
                     FGRVVVMGRGTVLLDGTPQEVFAERAWPTLASTSLTPPLPARIGARLGLGSTPTEAAL
                     VAALVARGNSDPIDAAVS"
     gene            complement(3697..4446)
                     /locus_tag="VIF63_01770"
     CDS             complement(3697..4446)
                     /locus_tag="VIF63_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238137.1"
                     /translation="MPALRAVDLSLAPGEILGVIGPNDSGKSTLGLVAAGLAPQAIGG
                     RLAGSVEVGGTPTRDLRPHELAQRVGILFQNPSTQLSSTARTVWEEVAFGPRNLGLPL
                     DEVVDWVASAIALLGIEGLAPRDPNRLSGGEGQLVALASVLALRPRVLVLDEPTSQLD
                     PAGTVLVGAALGRLRESGTAVVLVEHKADVLQSLAGRVAVLDEGRIVAIGSTRDLLAD
                     PLLEGLGVEPPSDVRIARALQSAGLPAGLVA"
     gene            complement(4521..5345)
                     /locus_tag="VIF63_01775"
     CDS             complement(4521..5345)
                     /locus_tag="VIF63_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEY8238138.1"
                     /translation="MNALGRERAAAAGSSLVPEFVRRRPPGGYAGLNPATKLVLAFAE
                     AAVAFGVGGWTGPVAALVTVLATAAVAGVLRRMAPFVVATLPLVVSIVLVNTFIYPGA
                     TDVFAEIGPFRGTWTGLTVALQAMLRVVAFALSVAVFALTTETDDLLTDLERRGLGRR
                     GVFVIGSAIATVPRMIERAGEIVDAQRARGLDTEGSVVRRLRGVIPLAGPMITGALSE
                     VEERTMALEARAFSAPGRRAVLRALPDSTGERIVRWSVAVLVVLAIGAGLAGVALP"
     gene            complement(5342..6172)
                     /locus_tag="VIF63_01780"
     CDS             complement(5342..6172)
                     /locus_tag="VIF63_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238139.1"
                     /translation="MSWGRFDTRTIVLIPIAIAINIVLGYTVQTVLKLPIYLDSIGTI
                     LVGVLAGPIPGAVTGALANLIWQYAPGIGGGTIGPFAITAAVIGLMAGIWGQLGVFRS
                     RPATGGQLALFALVAAAIVVLLAIPIIGNPAYTDPNIGGYPDWVYTAAIVVAAVAAIA
                     VAAFIYLRRDLAGFWVAIAGVVTGIVAAFVSAPISAIVFGGVTGNGTDLLVAALRQGG
                     ADVFNAALGQGLFSDPIDKTITSFVVFVILTSLSRRLIARFPLGERTLGPEPVEGTGA
                     "
     gene            complement(6231..6788)
                     /locus_tag="VIF63_01785"
     CDS             complement(6231..6788)
                     /locus_tag="VIF63_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8238140.1"
                     /translation="MSDPRRRPVTWSYSRDALERVRESIDLAEGRLGVAVLDDSGIAN
                     VLRTSRRIAVIGASSKPHRASNDILRYLIAAGYDVVPVHPNERQVEGLTCYRTLAEAV
                     LATGPVDIVDVFRRAEFCPGHAREAVAAGARCLWLQLGIVSAEAARIAASGGLSVVMD
                     RCIKVDHRRLVPPDPQVNGMPGAVP"
     gene            6969..8102
                     /locus_tag="VIF63_01790"
     CDS             6969..8102
                     /locus_tag="VIF63_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8238141.1"
                     /translation="MLHSRKAALLASAALLFVACGPGATAQPSAAPPSTSPTLAVTPV
                     PATVAPPTAAAVDYKACMVSDTGGIDDHAFNENAWKGMTDAQTQFGVEVKFLESAAET
                     DFEKNINQFLSEGCDMIVTVGFLLGDATKAAATANPDTRFAIVDFAYDPAIPNVEGLV
                     YSTNEAAMLAGYAAAAWSKTHVLGTFGGINIGGPVTDFMDGLVAGAHYFEKQKGTKTT
                     ILGWDAVAKTGSFTGNFSSIEDGKTIALGFLDENADVCLGVGGPIGQGCFAAIQERSA
                     AAVGLGVDVDWYLSVPQYKTLVLTSILKKIDVSVTAAVEHALNKVPAAPVFVSTLANG
                     GVDLGVFHDYDSQITAETKAEIDALKAAIIAGTVKVADYFPAP"
BASE COUNT         1295 a         2931 c         2864 g         1055 t
ORIGIN      
        1 gccgccggcg agccagacac cgagcaagga catcgggcgg ccgttcgagc cgctcgagga
       61 atgcaagacc ctcggcgact accggatgcg ctacgcacaa tatcggcgcg accccgacgt
      121 ccaggcggtc agtgccgcgc atccgatcat ctcgaccgtc gacgaccacg agttcgccga
      181 cggcgcctgg cgcgatggcg ccacggagca caagccggag cgcgatgggc cgtggtccga
      241 gcgaaaggcc gccgcgttcc gggcccggga ggagtggctg ccggtacgcc gccccgatcc
      301 cgccaacgcg gagcgggtct atcgctcggt cgcacttggc tcgccggccg acctgttcat
      361 gctcgacacg cggaccatgc gcgacgagcc cgtgccgccg ccggccctct cccgggaggg
      421 caggacgaac ctcggcgcgg accagaaggc gtggctcctg ggcgcgctcg ccgcgtcgaa
      481 ggcgcgctgg cggctgctcg gcaacccgtc ggtgatggcc cgaacctgga acgatgcgct
      541 gcccgagccg gcccgaaagg ccctggtcaa ggtcaagctc atcgacaccg acggtacggg
      601 ccctgactac gaccagtggg acggctaccc gcaggagcgc cgcgagctgc tgcgcgcgat
      661 cggcgagctc gatccaccga acgtggtcgt gttgagcgga gacgtccacg tgggcctcgc
      721 cgcggagctc gccgaggatc cgcacatgcc cccggtcgcg gtcgagttcg tgaacacgag
      781 cctgacctcg cagaacctcg acgacaagat gggctgggcg ccacgcacgg actccgtggt
      841 gatcgaggcc gcgctcctcg ccggcatgcc tcacctgcac tacgtcgacc tcgacagcca
      901 tggctactcc gtggtggacc tcgaccggga ccggctcagg ttcgagtggt ggacggtcga
      961 gcagctcgtg ggccgcgcgc cgggccagac gctcgccgcg tcactcgaag tccgccacgg
     1021 cgtggcccag ctggtcgggg acgccgccgt tcagccctga gcggccccgt ctggcagact
     1081 ggccgccatg cacacgagca cggcgaccga tcggtacgag gccgtcatcg gcatcgaggt
     1141 ccactgccag ctgcgcacgg cctcgaagat gttctgcgcc tgctcgacgg cgtacgacgg
     1201 cgcccggccg aacagccacg tctgcccggt ctgcctcggc ctgccgggcg ccctgccggt
     1261 catcaaccgg cgcgcggtcg agctggtcct cgcgaccggc gctgcgatcg gcgcctcgac
     1321 gcccgacgcg actcggtggg accgcaagaa ctacttctat ccggacttgc cgaagggcta
     1381 ccagatcagc cagtacgacc tgccgctcgc ggccgccggc accttgacgt tcgagacgag
     1441 cgatggcccg ttcacgaccc ggatcacgcg ggcccacctc gaggaggaca cggccaagct
     1501 cgtccacggc gagggcgagg cgggggagcg aaccagcctg gtcgacttca accgctcggg
     1561 catgccactc atggagatcg tcaccgagcc cgacctccgg acggccgagc aggcacgccg
     1621 ctacgccgag gagctccagc tcctgctgcg gaccatcggc gcgtccgacg ccgacatgga
     1681 gcgaggccag ctgcgcgtgg aggcaaacgt ctcgctgcga ccgaggggca cggaagcctt
     1741 cgggacccgc gtcgaggtca agaacatgaa ctccttccgt tcggtggagc gggcggtcga
     1801 gttcgagatc gagcgccagg cggcggcgct tgacgcgggc gaggcgctgc gaatggagac
     1861 ccgcggctgg tcggacgacc gcggcgagac ctaccacatg cgcaacaagg agacctccga
     1921 cgactaccgc tacttcccgg agccggacct gccgccgctc agggtggacg ccggctggct
     1981 ggcgcagatc cgcggcgagc tgccggagct gcccgccgcc cagcgggcgc gctaccggca
     2041 ggtcctcggg ctgtccgtgt atgacgcggc gctgctgacg gccgacccgg atgccggtcg
     2101 cctgttcgag gcgacgctgg tggccggcgg ggacgtcggg gccaagcccg tcgcgaactg
     2161 ggtgaccggc gactacctcc gaatgcgcaa tgcggcttcc gcgcccatca ccgtcgaccc
     2221 ggcagccctc gcggacctga tccgccgggt cgtcaacggc tcgctcaacc ggtcctccgc
     2281 ccgcgaggtg ctcgatgcac atgtggcgac tggcgagacg atcgaggcca tcgtcgcccg
     2341 gctcggcctc gcccagatct cggacgccgg tgcgctcggt tcgatcgtcg acgacgtgct
     2401 cgcggcgaac cccgccgccg ttgccgacat ccgcgcgggc aagccgcagg cgatcgggtt
     2461 cctcgtcggc caggtgatga aggcaacacg aggccaggcc aacagccagg tggcgagcgc
     2521 cgcgatccgg gcccgccttg gcatcgacga gcccggctga tgggcctggt cctcatcctc
     2581 gcgggcatcg ccgccactgc cgccggctac gcccggtttc gcgtcccctg ggcgaagtac
     2641 caggcactga agctgcagga ggcgaacatc gcccgttacg aatcgtggcg cggcggcctc
     2701 cgcgataccg ggccgaccgg cgcctccgtc gcgatggaga tgctccggaa gcaggcccgt
     2761 gacagcgccg tgatcatggc cgcaggtatc gcggtggtca tcctcgggtt cttcgtcagc
     2821 tgaccgcggc gtcgatggga tcgctgttgc cgcgcgcgac gagcgccgca acgagcgcag
     2881 cctctgtcgg ggtcgagccg aggccgagcc gcgccccgat ccgggcgggc agcggcgggg
     2941 tcagggaggt cgaggccagc gtcggccagg cgcgctcggc aaagacttcc tggggcgtgc
     3001 cgtcgaggag caccgtgccc cggcccatca ccaccacgcg accgaacgtc tcggctgcga
     3061 accggatgtc gtggctgatg gcaatgaccg tgcggccgtc cgcgatgagc tcggccacga
     3121 gccgctggat ccgcagcacg ccgcccgcgt cctggcccgt cgtcggctcg tcgaggatca
     3181 ggaccggcgt ctccatcgcg agtaccgagg cgatcgtcag cagcttgcgc ttcgagtagc
     3241 ccagatcgta cgggtgcgcc tcctcgaggc ccgccatgcc gacggcgtcg agggcgcgcg
     3301 cgaccgccgc atcaagggcc tcgccgcggc ggccgatgtt gcgggccccg aagccaacct
     3361 cggcgcgggc ggtctgggcg aagatctgcc gatcggggtt ctggaaggcg agcccgacga
     3421 gcctcgccag ctccgcgacc cgcctgtctg cgacgtcacg gccaccgacg aggacccggc
     3481 ccgccgttgg ccgcaggagg ccgttgaggt gtcgcaccag cgtcgacttc ccgctgccgt
     3541 tctgcccgat gagggcaacc gcctcaccga caccgaccgt caggctcacg ttcgtcagtg
     3601 cctgcgtgcc gtcgcgatac cgatagctga gcccctcgac cgcgacgtcg agaccggcgg
     3661 cgggacctcc gccggacggc ccggcgaccg agccgctcac gcgaccaacc cggcaggcag
     3721 cccagccgac tggagtgccc gtgcgatccg cacatccgac ggtggctcga cgccgaggcc
     3781 ctcgagcagg gggtcggcga gcaggtcgcg cgtcgacccg atcgcgacga tccttccctc
     3841 gtcgagcacc gcgacgcggc cggcgaggga ctgcaggacg tcggccttgt gctcgacgag
     3901 gacgacggcc gtccccgact cgcgcaggcg tccgagggcc gcgccgacga ggacggtgcc
     3961 ggccgggtcg agctggctgg tgggctcgtc gaggacgagc actcgcggcc ggagcgccag
     4021 caccgacgcc agggcgacca gctggccctc gccacccgac agccggttcg gatcgcgcgg
     4081 cgcgagtccc tcgatcccga gcagggcgat ggcggacgcg acccaatcca caacctcatc
     4141 gagcggcagg cccaggttcc gcgggccgaa ggcgacctcc tcccagaccg tccgggccgt
     4201 gctcgagagc tgcgtcgacg ggttctggaa gaggatcccg acgcgctgcg cgagctcgtg
     4261 cggccggaga tcgcgggtcg gggtcccgcc gacctcgacg ctacccgcga ggcggccgcc
     4321 gatcgcctgg ggagcgagcc ccgctgcgac caggccgagc gtcgacttgc ccgagtcgtt
     4381 cggcccgatg acgccaagga tctcgcccgg cgcaagcgac aggtcgaccg cgcggagggc
     4441 cggcatcgtc gatcctgcgt acgcatacgt gacgccccgg agcgccagcg cggctgcccc
     4501 ggggagtggc gtgtctggcg tcacgggagc gcgacgccgg cgaggccagc cccgatcgcc
     4561 aggaccacca gcacggcgac ggaccagcgc acgatgcgct cgccggtgga gtcggggaga
     4621 gcgcgcagga ccgcgcgccg tccgggggcg ctgaaggccc gcgcctcgag ggccatcgtg
     4681 cgctcctcga cttcgctcag cgcccccgtg atcatcggtc cggcgagcgg gatgacgccg
     4741 cggagccggc ggaccaccga tccctccgtg tcgagaccgc gcgcccgctg tgcgtcgacg
     4801 atctcgccgg cccgctcgat catgcgcggg accgtcgcaa tcgccgagcc gatcacgaac
     4861 acgcctcggc gcccgaggcc gcgccgctcg aggtcggtga gcaggtcgtc cgtctcggtg
     4921 gtgagggcga agaccgcgac ggaaagcgcg aacgcgacga cccggagcat cgcctgcagc
     4981 gcgaccgtca gcccggtcca ggtgccccgg aacggcccga tctccgcgaa cacgtccgtc
     5041 gcgccggggt agatgaacgt gttcacgagg acgatcgaga ccacgagcgg gagcgtcgcg
     5101 acgacgaacg gcgccatccg ccggagcaca cccgcgaccg cggccgtggc gagcacggtc
     5161 acgagcgccg cgaccggccc ggtccagcca ccgacgccga aggcgacggc cgcctcggcg
     5221 aacgccagca ccagcttcgt cgccgggttc aggcccgcgt acccgcccgg cggccggcgc
     5281 ctgacgaatt ccgggaccag cgaggagccg gccgcggccg cccgttcccg gccgagagcg
     5341 ttcaggcgcc ggtgccctcc acgggttcag gcccgagcgt ccgctcgccg agcgggaacc
     5401 gcgcgatcag ccggcgcgag aggctcgtga ggatgacgaa cacgacgaag ctggtgatcg
     5461 tcttgtcgat cgggtcgctg aacaggccct gcccgagcgc ggcgttgaac acgtcggcgc
     5521 cgccctgccg cagcgccgcg acgagcaggt ccgtgccgtt gccggtgacg ccgccgaaca
     5581 cgatcgcgga gatcggcgcg gagacgaacg cggcaacgat gccggtgacg acgcctgcga
     5641 tcgccaccca gaatccggcg agatcgcggc gaaggtagat gaacgcggcc acggcgatcg
     5701 cggccacggc ggcgaccacg atggctgcgg tgtagaccca gtcgggatag ccgccgatgt
     5761 tcgggtcggt gtaggccggg ttgccgatga tcgggatggc caggaggacg acgatcgccg
     5821 cggccacgag ggcgaatagc gcgagctggc cgcccgttgc cggccggctc cgaaagaccc
     5881 cgagctggcc ccagatcccg gccatcaaac cgatgaccgc cgcggtgatt gcgaacgggc
     5941 cgatcgtacc gcccccgatg ccgggcgcgt actgccagat gaggttggcg agcgcgccgg
     6001 tcacagcgcc ggggatcgga ccggcgagca ccccgacgag gatggtgccg atcgaatcga
     6061 ggtagatcgg cagcttgagg accgtctgga cggtgtaacc gaggacgatg ttgatggcga
     6121 tcgcgatcgg gatgagcacg atcgtccggg tatcgaagcg gccccagctc atgacactcc
     6181 ctcctccaga acgcgccctc gctcgggcgt cggctcccgc gcgagcgacc tcaaggtacc
     6241 gcgccgggca tcccgttcac ttgcggatcg ggcgggacga ggcgccggtg atccaccttg
     6301 atgcaccggt ccatgacgac ggaaagcccc ccactcgccg cgattcgcgc ggcctcggcg
     6361 ctgacgatgc cgagctgcag ccagaggcat cgggccccgg cggcgacagc ctcgcgggcg
     6421 tggcctgggc agaactccgc gcgccggaac acgtcgacga tgtcgacggg accggtggcc
     6481 aggacggctt ccgcgagcgt tcgatagcac gtcaggccct ccacctggcg ctcgttcgga
     6541 tggacgggca cgacgtcgta cccggccgcg atgaggtagc ggaggatgtc gttggacgcc
     6601 cggtggggct tcgacgacgc gccgatgacg gcgatgcgcc ggctcgttcg gaggacgttc
     6661 gcgatccccg agtcgtccag cacggcgacc ccgagccgac cctcggccag gtcgatcgac
     6721 tcgcgaacgc gctcgagagc gtcccgcgaa tacgaccagg tcacgggtcg ccgccgcgga
     6781 tccgacatac cgcggagcat tgcacacgcc attccccgcc gtcatactgc gccctgagcc
     6841 gaccgggtcc gcgaagggac ggaatgtccg cccgccccgg atcccgccga cagacgcact
     6901 ggatgtgcgc aggcagcccg ccctgagacc ttggctcgct cgggctgcgc agttgaggag
     6961 gagacacgtt gctgcactcg cgaaaggccg cgctcctggc gtcggccgca ctcctgttcg
     7021 tcgcctgcgg gccgggcgcc accgcccaac cgtccgcggc gccgccgtcg accagcccga
     7081 cgctggccgt gaccccggtg cccgccacgg tcgccccgcc gaccgccgcc gccgtcgact
     7141 acaaggcgtg catggtcagc gacacgggcg gcatcgacga ccacgcgttc aacgagaacg
     7201 cctggaaggg catgacggat gcccagaccc agttcggcgt cgaagtgaag ttcctcgagt
     7261 cggccgccga gaccgacttc gagaagaaca tcaaccagtt cctgagcgaa ggctgcgaca
     7321 tgatcgtcac cgtcggcttc ctgctcggtg acgccacgaa ggccgcagcg accgcgaacc
     7381 ccgatacccg cttcgccatc gtcgacttcg cctatgaccc ggccatcccg aatgtcgagg
     7441 gcctcgtcta ctcgacgaac gaagcggcca tgctcgcggg atatgccgcc gccgcctggt
     7501 cgaagaccca tgtcctgggc accttcggcg ggatcaacat cggcggaccg gtcacggact
     7561 tcatggatgg cctcgtcgcc ggcgcccact acttcgagaa gcagaagggc acgaagacga
     7621 ccattctcgg ctgggacgcc gtcgcgaaga ccggctcctt caccggcaac ttctcgagca
     7681 tcgaggacgg gaagacgatc gcccttgggt tcctcgacga gaacgcggac gtctgcctcg
     7741 gtgtcggtgg accgatcggt cagggctgct tcgctgccat ccaggagcga agtgccgcag
     7801 ccgtcgggct cggcgtcgac gtcgactggt acctgtcagt gccgcaatac aagaccctgg
     7861 tcctgacctc gatcctcaag aagatcgatg tcagcgtgac tgcggctgtc gagcacgcgc
     7921 tgaacaaggt cccggctgcg ccggtattcg tgagcacgct ggccaatggc ggcgtggacc
     7981 tcggtgtctt ccacgactac gactcgcaga tcacggccga gacgaaggcc gagatcgacg
     8041 ccctcaaggc agcgatcatc gcgggcaccg tgaaggtcgc ggactacttc ccggcgccgt
     8101 agtcagagcg tcgcgttcca acgcgataac cggcggtggg ggggg
//
LOCUS       DATBSW010000067        10527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_921961, whole genome shotgun sequence.
ACCESSION   DATBSW010000067 DATBSW010000000
VERSION     DATBSW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921961"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VIF63_01795"
     CDS             <1..424
                     /locus_tag="VIF63_01795"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8238142.1"
                     /translation="GVVVSADRVRPSLVIGMRRRGRDSVTWTLPKGTPDPGETTEETA
                     LREVREETGLEVRILEPLPSIEYAFAQDGTRIRKTVHYFLMEPTGGDLSRHDHEFERV
                     RWVPFDEAPMLLSFATERELVATAQARLGEKQGASAPS"
     gene            421..1554
                     /gene="gcvT"
                     /locus_tag="VIF63_01800"
     CDS             421..1554
                     /gene="gcvT"
                     /locus_tag="VIF63_01800"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY8238143.1"
                     /translation="MTDAEPAAPAAAPELHETALIDIHRALGARLIEFGGWLMPVQYG
                     GILEEHRAVRERAGLFDLSHMGELFVEGDDAGDALAAALVTDPRTLAVGRAQYSMICA
                     PDGGIIDDLIVYRLGPERFLVVANASNAPVVSEELAARLVRWRAILDDQSLVTSLVAI
                     QGPRAGGILAPLTDIDLTALRYYAIAEGQVAGVPALVARTGYTGEDGFEVFVDWKRGP
                     EIWEALAKAGAKDGVMACGLGARDTLRLEAGMPLYGNELDRQTNPFDAGLGRVVKLEK
                     QGDFVGREALEKVAADGPKKRLVGLTITGRGIARHGYPVVAGDRPTGVVTSGTQSPTL
                     GVPIAMAYVAPTDAEPGTILAVQVREQPVSAEVVPLPFYKRAK"
     gene            1612..1992
                     /gene="gcvH"
                     /locus_tag="VIF63_01805"
     CDS             1612..1992
                     /gene="gcvH"
                     /locus_tag="VIF63_01805"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY8238144.1"
                     /translation="MVPADLRYTKDHEWVRVEGDTATIGVTEFAAHELGDVVFVDLPA
                     VGKSVDQSATFGVVESVKAVSDLYAPVTGEVVEVNGDLAGAPELVNSDPFGAGWMIKV
                     RIADDAQVSGLLDAAAYEQLTAEA"
     gene            2167..3096
                     /locus_tag="VIF63_01810"
     CDS             2167..3096
                     /locus_tag="VIF63_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238145.1"
                     /translation="MVGRSIREIVWARLRRDKVAMVCLVVLAIYTILAVIGPNLLSAL
                     GWDPYQLNRDAVDSAKGGLPTLPFGGISAAHPLGVESGTGRDIMAQLVNGLRISLIIA
                     ASSTLLTVIIGTFVGIIAGYFGGLTDTLTGRFMDLILAFPFLLLVLALSNPLTQRLTD
                     LGVPAGNESRVLYLVLAFSIFGWPYLARIVRGQVLSIREREFVEAAVAMGAGRRRILF
                     GEILPNLWAPILVYATLTLPAYISFEAAISFLGVSVQAPDVTFGGMLANSVRFFNVVP
                     TYLLIPGTALVILVVAFNLLGDALRDALDPKSH"
     gene            3217..5049
                     /locus_tag="VIF63_01815"
     CDS             3217..5049
                     /locus_tag="VIF63_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8238146.1"
                     /translation="MVRSKAWLLRALTFGMTAALVVAACGGTPAPSTVTGTPGPVATG
                     PAATGPTATEDTGPQKGGTIYLLTNADNWVDVDPQRVYTGEDAAFFGGTIYRGLVSYV
                     YSPDEVEGTKITGDLATDTGTTTDGGKSWSFTLRDGVSWQDGSALTCDDVAYGISRQY
                     ATDIMGGGPTYGIQFLDIPLNDDGTSQYPGPYTATADQQALFDKAAACNGNTITFTLN
                     NPRGDFAYSTLWGSFPVPDPKGHPEMPDIGEGYGITTPPWASGPYKVESYTPGKGGSM
                     ILVRNDGWKQESDPVRPALPDKWEIDFGLEPQLIDQRLMNPSGNDEFAIQYGGLQPTN
                     LPTVFKDNETPQDAFAGRAISGFDPYVRYWWVDINKIPNEKIRAALGIALDREAVRTV
                     LGGDFYGDYGNGVLKPNIGADYADTGYYTDMFGEAIPTTGNPELAKKLIAESGEPAPT
                     LTWNFADTPVGQQYFAVVQSSLAKAGITVNPGAIPPGDYYKVVFDPDNELTGEFGNTG
                     WGPDWPNAYTVLAPLFTEIGGWDLSKVKDAAYEAELKTIVETLDRGEQAAKWQALNKK
                     VVQSGWIIPTFFGRSYTLAGTKVGPIYRWPGAGSWPYGIMGVTK"
     gene            5161..6168
                     /locus_tag="VIF63_01820"
     CDS             5161..6168
                     /locus_tag="VIF63_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238147.1"
                     /translation="MLGFLIRRVALMVTLLFMLTIVVFVLFSLLPGEPARLYCGKSCT
                     PTAIDAINHRLELDIPIVDQYTKYVSGIFFGRTFSPTSPQPIECPAPCLGYSYDKHVP
                     VLTLITRAAPVTFWLSIGAFIIWIVLGVSLGIFAALKRGRWPDRLVVGISLVGYSMPS
                     FFIGLLLYVFVIISLKLLPLPSYPQGGWTAPFTSPVQFLQTMILPWIVLATLYAAFYM
                     RLTRNQVLEVLGDDYVRTARAKGLPERTVVVRHALRAGLTPIVTAAGLDLAGLLGGAI
                     ISESIFSLPGMGSLAIGAITKSDLPLIAGITLLSSAIIIFANFIVDLLYAVIDPRVRI
                     T"
     gene            6195..7310
                     /locus_tag="VIF63_01825"
     CDS             6195..7310
                     /locus_tag="VIF63_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238148.1"
                     /translation="MSSVPEPYLTVNDLHVQFPTEDGLVTAVDSVSVSVARGKTLAIV
                     GESGSGKSVTALAIMGLLNRKQAKVSGQVWLDGEELLDKSDEGVRRLRGLKMAMIFQD
                     PMSSLHPFYRVGGQLTEAMQVHRKLTKKQARNEAIEMLNRVGIPAPKRRVDDYPHQLS
                     GGMRQRVMIAMALVNSPALLIADEPTTALDVTVQAQILDLIAQLQKEFNTAVVLITHD
                     LGIVADVADEVAVMYAARVVEQAPTSRLYANPEMPYTLGLLSSIPRMDRVRTDRLDPI
                     PGNPPSPLRHPSGCVFHTRCKYTDRVPGERCFNDRPELLESEPTHSVRCHIPSEERRQ
                     IAESVLSMGKAPIATAGGGAPLAGAGTVVESVGGAPA"
     gene            7307..8440
                     /locus_tag="VIF63_01830"
     CDS             7307..8440
                     /locus_tag="VIF63_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238149.1"
                     /translation="MTGTMDSATTPGVAPKEILRVTDMRKHFPVKARFAGVLSRTVGQ
                     VKAVDGISLSLNAGETLGLVGESGCGKSTAGRTILRLLAPTGGTITFDGQDITKLSGN
                     ALMPLRRKAQIIFQDPYTALNPRHTVGTIISAPFNIQGVRPPGGVKAAVNELMERVGL
                     NPEHYNRYPNEFSGGQRQRIGIARAVALRPKFIVCDEPVSALDVSIQAQILNLLKDLQ
                     DDFGVAYLFVAHDLAVVRQISHRVAVMYLGKVMESAPREVIYAEPLHPYTHSLLSAVP
                     IPDPKRQRNRGRILLQGDLPSPIDPPSGCVFRTRCFQAQEKCAVEVPPLIELAPGHHV
                     ACHFPIAAANLPKRIEVAAAEAEETGPAVPAPAVDVPVQGAGV"
     gene            complement(8437..8967)
                     /locus_tag="VIF63_01835"
     CDS             complement(8437..8967)
                     /locus_tag="VIF63_01835"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8238150.1"
                     /translation="MELVYETHAITTDNEAGIATGWLPGVLSAAGRRTAAELGDRRRR
                     DGIVAVYASDLARAVETARIAFEGTAIPIRLDPRLRECNYGRLNGMPRDRLDLERTRH
                     LDEPWPDGESYADVVARTGDLLVDLVPQWDGARILVIAHSANQWALDHLLLGADMAER
                     IAAGMTWQPGWEYVLG"
     gene            8996..10021
                     /locus_tag="VIF63_01840"
     CDS             8996..10021
                     /locus_tag="VIF63_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8238151.1"
                     /translation="MQPLRIALAQLAPRLGQLEGNLERHHALLREARSGGAGLVVFPE
                     LGLTGYLLSDLAAEVALRLDDPRLAELAAETADGLSAVVSFVEESGDHRLFIAAALME
                     DGEIRHVHRKVYLPTYGQFDERRFFAAGDTIRATPSRLGVGVGIAVCEDFWHLQTPQL
                     LALDGAQLLINVSSSPGRDLAARNEVGLGTATSWRTLMRTYAQLTGSFVVFVNRVGVD
                     ESMTFWGGSEVIAPSGDPIFSAPFFDDELYLVDVNLDDIRRERIALPLLRDERLELQA
                     RELGRIIAERAGLAADTTAESGAGPGLDVAPERKRARAVAQEAESDARAPEAGPPEPI
                     RFRSRRR"
     gene            10018..>10527
                     /gene="nadE"
                     /locus_tag="VIF63_01845"
     CDS             10018..>10527
                     /gene="nadE"
                     /locus_tag="VIF63_01845"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY8238152.1"
                     /translation="MSTVGALPTDDAGKPLFDLPDELAIDTDVARRVIAGFIRGQLEQ
                     AGFERCVLGLSGGIDSALVAYLVAEAIGADKLLCVLMPYRTSSPASRGDAETIVADIG
                     CASELVEITAMVDGYFGSDAAVGAGGEGARDASALRRGNLMARMRMSVLYDRSVAFGG
                     LVVGTGNKTE"
BASE COUNT         1646 a         3718 c         3502 g         1661 t
ORIGIN      
        1 cggggtcgtc gtcagcgcgg atcgagttcg accatcgctc gtcatcggca tgcgccggcg
       61 cggtcgtgat tcggtgacgt ggacgctgcc caagggcacg cccgatcccg gcgagaccac
      121 cgaggagacg gcgctgcgcg aggtccgcga ggagaccggg ctcgaggtcc gcatcctcga
      181 gccgctgcca tcgatcgagt acgccttcgc ccaggacggc acgcgtatcc gcaagaccgt
      241 gcactacttc ctcatggagc cgacgggcgg cgacctctcg cgccacgacc acgagttcga
      301 gcgcgtgcgc tgggtgccgt tcgacgaggc gccgatgctg ctgagcttcg ccaccgagcg
      361 cgagctcgtc gcgacggccc aggcacggct cggtgagaag cagggcgcga gcgccccatc
      421 gtgaccgacg ccgaaccggc ggccccggcc gcagcaccgg agctccacga gaccgccctc
      481 atcgacatcc accgagccct gggtgcccgc ctgatcgagt tcggcggctg gctcatgccg
      541 gtccagtacg gcggcatcct cgaggagcac cgagcggttc gcgagcgcgc cggcctgttc
      601 gacctgtcgc atatgggcga gctgttcgtc gagggcgacg acgccggcga cgcacttgca
      661 gcggcgctcg tcaccgatcc gcgcacgctc gcggtcgggc gggcgcagta ctcgatgatc
      721 tgtgcgccag acggcgggat catcgacgac ctcatcgtct accggctcgg gccggagcgg
      781 ttcctcgtcg tcgccaacgc gagcaacgca ccggtcgtgt cggaggagct tgccgcccgg
      841 ctcgtgcgct ggcgagccat cctcgacgac cagtcgctgg tgacctcgct cgtcgcgatc
      901 caaggtccgc gcgccggcgg catcctcgcg ccgctgaccg acatcgactt gaccgcgctc
      961 cgctactacg ccatcgccga aggccaggtc gcgggcgttc ccgcgctcgt cgcccggacc
     1021 ggctacacgg gcgaggatgg attcgaggtc ttcgtcgact ggaagcgcgg gccggagatc
     1081 tgggaggcac tcgcgaaggc cggcgccaag gatggggtga tggcctgcgg gctgggcgcg
     1141 cgcgacacgc tgcgcctcga agcggggatg ccgctgtacg gcaacgagct cgatcgccag
     1201 acgaatccct tcgacgcggg gctcggtcgc gtcgtgaagc tcgagaagca gggcgacttc
     1261 gtgggccggg aggccctcga gaaggtggcc gcggacggcc cgaagaagcg gctggtcggc
     1321 ctgaccatca ccggccgcgg gatcgcgcgc cacggctacc ccgtcgtcgc cggcgatcga
     1381 cccacggggg tcgtcaccag cggcacccaa tccccgacac tgggcgtacc catcgcgatg
     1441 gcgtacgtcg cgccgaccga tgccgagccg ggtacgatac tggccgtcca ggtgcgcgag
     1501 cagccggtct ccgccgaggt ggtgccgctg ccgttctaca aacgagcgaa gtagccccgc
     1561 gccagcatcg agcgctcgcc gcggctcgcg agccgtcgat cggaggaatg gatggtccct
     1621 gccgacctgc gctacacgaa ggaccacgag tgggtccgcg tcgagggcga caccgccacg
     1681 atcggcgtga ccgagttcgc ggcgcacgag ctcggcgatg tcgtcttcgt cgacctgccg
     1741 gcggtgggga agtccgtcga tcagtccgcc acgttcggcg tcgtcgagtc ggtgaaggcc
     1801 gtgagcgacc tgtacgcacc cgtcacgggt gaggtcgtcg aggtcaatgg cgacctggcg
     1861 ggcgcgccgg agctcgtgaa cagcgacccg ttcggcgccg gctggatgat caaggtccgg
     1921 atcgccgacg acgcacaggt ctcgggcctg ctcgacgccg cggcgtacga gcagctgacc
     1981 gcggaggcct gaccgaccct ggcgcacggc tcgcccgcgc ccgctgggtc ggcgacgtcc
     2041 ataaagaaat cgcaagcaat ccgcgcgctc gatcggcacc ccgttcggcg cctgtccggc
     2101 cccgcagacc ggttgggagc cccagcccgc gtttgacagg ggtctcgcgt cggctattct
     2161 ccgcctgtgg tcggccgatc catccgcgag atcgtctggg cgaggcttcg ccgcgacaag
     2221 gtcgcaatgg tctgtctcgt cgtcctcgcg atctacacca tcctggccgt catcgggccg
     2281 aatctcctca gcgcgctcgg atgggatccc taccagctca atcgcgacgc ggtcgactcg
     2341 gccaagggcg gtctgcccac gttgccgttc ggcgggatca gcgcggcgca tcccctgggc
     2401 gtcgaatcgg ggacgggccg cgacatcatg gcccagctcg tcaacggcct ccgcatctcg
     2461 ctcatcatcg ccgccagctc gacgttgctg acggtcatca tcgggacgtt cgtcggcatc
     2521 atcgccggct acttcggggg cctgaccgac acgctcaccg gccggttcat ggacctgatc
     2581 ctcgcgttcc cgttcctgct cctcgtcctg gccctctcga accccctgac ccagcgcctg
     2641 accgacctgg gcgtgccggc cggcaacgag tcccgggtgc tgtacctggt gctcgccttc
     2701 agcatcttcg gctggccgta cctcgcccgc atcgtgcggg gccaggtcct cagcatccgc
     2761 gagcgggagt tcgtggaggc cgcggtcgcg atgggtgccg ggcgccggcg aatcctgttc
     2821 ggcgagatcc tgcccaacct ctgggcgccg atcctcgtct acgcgacgct gacgctgccc
     2881 gcgtacatca gcttcgaggc cgcgatctca ttcctcggcg tgagcgtaca ggctcctgac
     2941 gtgacattcg gcggcatgct cgcgaactcg gtgcgattct tcaatgtcgt cccgacatac
     3001 ctgctcatcc cgggcaccgc gctcgtgatc ctcgttgtcg cattcaatct tctgggcgac
     3061 gcgcttcggg acgcgctcga ccccaagtcc cactgaggtc gggccccacc ccgacctccc
     3121 cgatcactgg aggaggccta ctcgcaatag ttgctcggct gtgtatgatc cgtcatcgcg
     3181 tgctccggga cgatcccggc ccgttggagg aagacaatgg ttagatccaa agcctggttg
     3241 ctccgcgccc tgacattcgg catgaccgcc gccctcgtcg tcgccgcgtg cggtggcacg
     3301 ccggctccga gcacggtcac cggcacccca gggccagtcg cgacagggcc agcggccaca
     3361 gggccgacgg cgacagagga caccggcccc cagaagggcg gcacgatcta tctgctgacc
     3421 aatgcagaca actgggtcga cgttgacccg cagcgcgtct acaccggcga ggacgctgcg
     3481 ttcttcggcg gaacgatcta ccgcggcctc gtgtcgtacg tctactcgcc cgacgaggtc
     3541 gaggggacga agatcacggg cgacctcgcg actgataccg gaaccaccac tgacggtggc
     3601 aagtcgtgga gcttcacgct tcgcgacggc gtcagctggc aggacggcag tgcgctcacc
     3661 tgcgatgacg ttgcatacgg catctcacgc cagtacgcga ccgacatcat gggcggcggt
     3721 ccgacctacg gcatccagtt cctggacatc ccgctcaacg acgacgggac cagccagtac
     3781 cccggtccct acacggcgac cgcggaccag caggccctct tcgacaaggc ggcggcctgt
     3841 aacggcaaca cgatcacctt caccctgaac aacccgcgtg gcgacttcgc ctactcgacg
     3901 ctgtggggct cgttccccgt cccggatccg aagggtcacc cggagatgcc ggacatcggc
     3961 gagggctacg ggatcaccac cccgccgtgg gcgagcggcc cgtacaaggt cgagagctac
     4021 acgcccggca agggcggctc gatgatcctt gttcgcaacg atggctggaa gcaggaaagc
     4081 gaccccgtcc ggccggcact tcccgacaag tgggagatcg atttcggcct ggagccacag
     4141 ctgattgacc agcggctcat gaacccctcg gggaacgatg agtttgccat tcagtacggc
     4201 ggcctccagc ccacgaacct tccgaccgtc ttcaaggaca acgagacccc ccaggatgca
     4261 tttgcggggc gggccatcag cggctttgac ccgtacgtcc ggtactggtg ggtcgacatc
     4321 aacaagatcc ccaacgagaa gatccgcgcg gcgctcggca tcgccctcga ccgcgaggcg
     4381 gtccggacgg tgctcggcgg cgacttctac ggtgactacg gcaacggcgt gctcaagccg
     4441 aacatcggcg ctgactatgc cgataccggg tactacaccg acatgttcgg tgaggccatc
     4501 cccaccaccg gcaatcccga gttggccaag aagctgatcg ccgagtccgg cgagccggct
     4561 ccgacgctga cctggaactt cgctgacacc ccggtcggcc agcagtactt tgcggtcgtc
     4621 cagtcctcgc tcgccaaggc gggcatcacg gtcaaccccg gcgccatccc gcccggtgac
     4681 tactacaagg tggtcttcga tccggacaat gagctgaccg gtgagttcgg caacacgggc
     4741 tggggtcccg actggccgaa cgcctacacg gttctcgcgc cgctcttcac ggagatcggt
     4801 ggctgggacc tctcgaaggt caaggacgca gcctacgagg ctgagctcaa gaccattgtc
     4861 gagacccttg atcggggcga gcaggctgcc aagtggcagg ccctcaacaa gaaggtggtc
     4921 cagagtggct ggatcatccc gacgttcttc ggccgcagct acacgctggc gggcacgaag
     4981 gtcggtccga tctaccgttg gccgggcgca ggctcgtggc cgtacggcat catgggcgtc
     5041 accaagtagc cctggctccg ccagagcgca gccaggaccg caggtcctga catccggggg
     5101 gagcgggtca ccgctccccc cggagcacac cacccggaaa tctgaacgag ggcaggcagc
     5161 gtgctcggat tcctgatccg tcgggtcgct ctcatggtca ctctcctgtt catgttgacc
     5221 atcgtggtct tcgtcctgtt cagcctgctg ccgggtgagc cggcgcgcct ctactgtggc
     5281 aagtcctgca cgccgaccgc gatcgacgcg atcaatcacc ggctcgagct ggatatcccg
     5341 atcgtcgacc agtacacgaa gtacgtctcg ggaatcttct tcgggcgcac gttctcaccg
     5401 acctccccgc aaccgatcga atgtcccgca ccgtgcctcg gctactcata cgacaagcac
     5461 gtcccggtgc tgaccctgat cacgcgcgcc gcgccagtga ccttctggct ctcgatcggg
     5521 gcattcatca tctggatcgt gctcggcgtt tcgcttggga tctttgcggc cctcaagcgc
     5581 ggccgctggc cggacaggct ggtggtgggg atatcgctcg tcggctattc gatgccgtcg
     5641 ttcttcatcg gcctcctgct gtacgtgttc gtgatcatct ccctgaagct gctgccactc
     5701 ccgtcatacc cgcagggagg ctggacggcg ccgttcacga gcccggtcca gttcctgcag
     5761 acgatgatcc tgccgtggat cgtcctggcg acgctgtacg cggcgttcta catgcggctg
     5821 acccgcaacc aggtcctcga ggtcctcggc gatgactacg tgcggaccgc acgcgccaag
     5881 ggcctgcccg agcggacggt cgtggtcagg cacgcgctcc gcgccgggct gaccccgatc
     5941 gtcacggcgg ccgggctcga tctcgcgggt ctgctgggcg gcgcgatcat ctcggagagc
     6001 atcttcagcc tgcccggcat gggatcgctg gcgatcgggg cgatcaccaa gtccgacctg
     6061 ccgctgatcg cgggcatcac gctgctgagc tccgcgatca tcatctttgc caacttcatc
     6121 gtcgacctgc tgtacgcggt gattgaccca cgcgtgcgca tcacatgagg cagccggaca
     6181 accaacgcgc cgacatgtcg tctgtccccg agccctatct caccgtcaac gatctccacg
     6241 tccagttccc aaccgaggac ggcctcgtca cggcggtcga ttccgtctcg gtgtcggtcg
     6301 cccgcggcaa gaccctcgcc atcgtcggcg agtcgggctc gggcaagagc gtcaccgccc
     6361 tggcgatcat gggcctgctc aaccgcaagc aggcgaaggt cagcggccag gtgtggctcg
     6421 acggcgaaga gctgctggac aagtccgacg agggcgtccg gcggctgcgt ggcctgaaga
     6481 tggcgatgat cttccaggac ccgatgtcca gcctccaccc gttctaccgg gtcggtgggc
     6541 agctgacgga ggcgatgcag gtccatcgca agctcacgaa gaagcaggcc cgcaacgagg
     6601 cgatcgagat gctcaaccgg gtgggcatcc ctgcgccgaa gcgtcgcgtc gacgactacc
     6661 cgcatcagct gtccggcggc atgcgccagc gcgtgatgat tgccatggcg ctcgtcaact
     6721 ccccggcgct gctgatcgcg gacgagccaa cgaccgcact cgatgtgacg gtccaggcgc
     6781 agatcctcga cctgattgcc cagctccaga aggaattcaa taccgccgtc gtgctcatca
     6841 cccacgacct cggcatcgtc gcggacgtcg ccgacgaggt tgccgtcatg tacgccgccc
     6901 gcgtcgtcga gcaggccccg accagccgcc tgtacgcgaa cccggagatg ccatacacgt
     6961 tgggcctgct ctcgtcgatt ccgagaatgg accgcgtgcg gaccgatcgg ctcgatccga
     7021 tcccgggcaa cccgccgtca ccccttcggc atccgtccgg ctgcgtcttc cacacgcgct
     7081 gcaagtacac cgaccgggtg ccgggcgaac ggtgcttcaa cgaccgaccg gagctcctgg
     7141 agagcgagcc cacacattcc gtgcggtgtc acatcccaag cgaggagcgc cgccagattg
     7201 ccgagagcgt cctgagcatg gggaaggcgc cgatcgccac cgccggcggc ggggcacccc
     7261 tcgccggcgc cggtaccgtg gtcgagtccg tgggcggagc gcccgcatga cggggacgat
     7321 ggactcggcg accacccccg gcgtcgcgcc caaggagatc ctccgggtca ccgacatgcg
     7381 caagcacttc ccggtgaagg cacgcttcgc cggcgtgctg tcccggaccg tcggccaggt
     7441 gaaggccgtt gacggcatca gcctctccct caacgccggc gagaccctgg gcctcgtggg
     7501 cgagagcggc tgcggcaagt cgacggccgg tcggacgatc cttcggctgc tcgcgccgac
     7561 gggcggcacc atcacgttcg acggccagga catcacgaag ctgtcgggga acgcgctcat
     7621 gccgctgcgt cgcaaggcgc agatcatctt ccaggacccg tacaccgcct tgaacccgcg
     7681 gcacacggtg ggcacgatca tcagcgcgcc cttcaacatc cagggcgtca ggccgccggg
     7741 tggcgtcaag gccgccgtca acgagctgat ggagcgggtc ggcctcaatc cggagcacta
     7801 caaccgctat ccgaatgagt tctccggcgg ccagcggcag cgcatcggca tcgcgcgcgc
     7861 cgtcgccctg cggccgaagt tcatcgtctg cgacgagccc gtgtcggcgc tcgacgtctc
     7921 gatccaggcc cagatcctga acctgctcaa ggacctccag gacgacttcg gcgtggccta
     7981 cctgttcgtg gcccacgacc tcgccgtcgt ccggcagatc tcgcaccgcg tcgcggtgat
     8041 gtacctcggc aaggtcatgg agagcgcgcc gcgcgaggtg atctacgcgg agccgctcca
     8101 tccgtacacg cactccctgc tctcggccgt gccgatcccg gacccgaagc ggcaacggaa
     8161 ccgcggtcga atcctgctcc agggcgacct gccgagcccg atcgacccgc cgagcggctg
     8221 cgtcttccga acgcgctgct tccaggcgca ggagaagtgc gcggtcgagg tgccgccgct
     8281 catcgagctc gccccgggcc accacgttgc ctgccatttc ccgattgccg ccgccaacct
     8341 gccgaagcgc atcgaagtgg ccgcggccga ggccgaggag accgggccgg cggtgccggc
     8401 acccgccgtc gacgtgccgg tccagggcgc aggcgtctaa ccgagaacgt attcccaacc
     8461 gggctgccag gtcatcccgg ccgcgatccg ctcggccatg tcggcgccga gcagcaggtg
     8521 atcgagcgcc cactggttcg cggagtgggc gatgacgagg atgcgggcac cgtcccactg
     8581 cggaacgagg tcgacgagca gatctccagt gcgcgccacg acgtcggcgt agctctcgcc
     8641 gtccggccag ggttcgtcga ggtggcgggt ccgctcgaga tcgaggcggt cgcggggcat
     8701 gccattgagc cggccgtagt tgcactcgcg gagcctcggg tcgaggcgga tcgggatggc
     8761 cgttccctcg aacgcgattc gggcggtttc gacggcccgc gccaggtccg aggcgtagac
     8821 cgccacgatc ccgtcccggc gccgacggtc gcccagctcg gccgcggttc gccggccggc
     8881 cgccgacagc acgcccggca gccagccggt cgcgatgccc gcctcgttgt cggtcgtgat
     8941 cgcgtgggtc tcgtagacca gctccatcgc cacggcggaa ggataggatc agcacgtgca
     9001 gccactccgg atcgccctcg cccagcttgc gccgcgcctc gggcagctcg agggcaacct
     9061 cgagcgccac cacgcgctcc tgcgcgaagc acggagcggc ggcgccggac tcgtggtgtt
     9121 cccggagctg ggactgaccg gctatctcct ctcggacctc gccgccgagg tcgcgctgcg
     9181 cctcgacgac ccgcgcctcg ccgagctcgc cgcggagacc gccgatgggc tttcggcggt
     9241 tgtgtccttc gtcgaggagt ccggcgacca ccggctgttc attgccgccg ccctcatgga
     9301 ggacggcgag attcggcacg tccaccgaaa ggtctacctg ccgacctacg gccagttcga
     9361 cgagcgccgg ttcttcgcgg ccggcgacac gatccgggcg acgccgtcgc ggctcggcgt
     9421 gggcgtgggc atcgcggtgt gcgaggactt ctggcacctg cagacgccgc agctgctcgc
     9481 cctcgacggc gcccagctcc tgatcaatgt ctcgtcctcg ccgggccgcg accttgcggc
     9541 gcgcaacgag gtgggcctgg gcaccgccac gtcgtggcgg acgctgatgc gtacctacgc
     9601 gcagctcacc ggatcgttcg tcgtgttcgt caatcgggtt ggggtggacg agtcgatgac
     9661 gttctggggc ggatccgagg tcatcgcgcc gtcgggcgat ccgatcttct ccgcgccgtt
     9721 cttcgacgac gagctgtacc tcgtcgacgt gaacctcgac gacatccgcc gcgagcggat
     9781 cgcgctgcca ctcctgcgcg acgagcggct cgagctccag gcgcgcgagc tcggccggat
     9841 catcgccgag cgggccggcc ttgccgccga caccacggcc gagtcgggtg ccgggccggg
     9901 cctcgacgtc gcgccggaac ggaagcgagc gcgagcggtg gcgcaggagg ccgaatcgga
     9961 tgcaagggcg cccgaggcag gtccgcccga gccgatccgc ttcaggtccc ggcgccgatg
    10021 agcaccgtcg gcgcgctgcc caccgacgac gccggcaagc cgctcttcga cctgcccgac
    10081 gagctggcga tcgacaccga cgtggcgcga cgcgtgatcg ccgggttcat ccgaggccag
    10141 ctcgagcagg ccggcttcga gcgttgcgtg ctcggcctgt cgggcgggat cgactcggcg
    10201 ctcgtggcgt acctcgtcgc cgaggcgatc ggcgcggaca agctgctgtg cgtgctgatg
    10261 ccatatcgga cgtcgtcgcc cgcgtcgcgc ggcgacgccg agacgatcgt ggctgacatc
    10321 ggctgcgcca gcgagctcgt cgagatcacg gcgatggtcg acggctactt cgggagcgac
    10381 gcggccgttg gcgccggcgg cgagggcgcg cgcgacgcgt cggcgctgcg acggggcaac
    10441 ctcatggccc ggatgcggat gtccgtgctg tacgaccggt cggtggcgtt cggcggcctg
    10501 gtggtcggga ccggaaacaa gaccgag
//
LOCUS       DATBSW010000068         5634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_146223, whole genome shotgun sequence.
ACCESSION   DATBSW010000068 DATBSW010000000
VERSION     DATBSW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146223"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIF63_01850"
     CDS             <1..462
                     /locus_tag="VIF63_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8238153.1"
                     /translation="LELAGIDAVTRRARVAELLETVGLEAAAAQVPSQLSGGMRQRAA
                     LARTLALEPQVLLLDEPFSALDELTRERLNLELLAITARTPTTILLVTHSVQEAIFLA
                     DRVVVLSERPGRVAADVPVDLARPRALADLDAAVVTETARLVRAHLGEAAA"
     gene            479..1261
                     /locus_tag="VIF63_01855"
     CDS             479..1261
                     /locus_tag="VIF63_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238154.1"
                     /translation="MTTLAATRPTTTRIPGAITAALALAAFLGLWKGIVLVSGFPPFI
                     LPPPEQVAERFVGAWADGTIGPHFVRTLGEVLVGFGLGAWSALFVGYALARLPLAERL
                     LSPYLVAAQAIPILVLAPLLALWFGTGLTAKLVICALIVFFPVAITTMVGVRGVDARL
                     LELARSLRATRWQVIRTVEIPAALPQIFGGLRVGVTLAVVGAIVGEWTGADHGLGLLM
                     NLARGSLFDTPLLFATLVTVALLGVVLYGLVVLIERRLVGPR"
     gene            1288..2349
                     /locus_tag="VIF63_01860"
     CDS             1288..2349
                     /locus_tag="VIF63_01860"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8238155.1"
                     /translation="MSRRALSLLAAATLLVVACGPRAEPSTCSDALPTAGGCGFQPTP
                     DVTGGPPVALKVGLGYIPSVQFAPFYYAQQAGYYTEAGLEVTFENKIDPDLVTLVGQG
                     AIDIGVADGTSVIPAVSQGIPVRYIATIYGTYPSIVFAKASSGITKAADLEGKKIGIP
                     GKYGSSWIMLQALLGSADLKPDDAAIVEYPDFGQGVAVQAGAVDAATGFANNEPVQLE
                     LTGTKAVILHVDDVVALPGPGLISGNDALGEEKAEAIATFVSVTLRAMTEIAANPALG
                     LDAAIAAVPELATSRETQAAILDATIASWSPAGGGADFGTIDRAGWEASLAYLTTLGL
                     VPNPVVVDDLIRDDLLPSG"
     gene            2386..2931
                     /locus_tag="VIF63_01865"
     CDS             2386..2931
                     /locus_tag="VIF63_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY8238156.1"
                     /translation="MAMRKFLAGASDRPFPTDAHDTETVRRIAGQLDALPVDRARFLA
                     AFAYILTRAAAADLEISAVESEAIERLVAEHGGLPDAQAVLVAQIARNQALLYGGTED
                     YLVTRQFRDLASAEDRLALLRCCYLVGAADDTITAEESNLLQQIARELDIDRVAVNAI
                     RTEFAPKLAAMQRMLHLREAE"
     gene            complement(3246..3509)
                     /locus_tag="VIF63_01870"
     CDS             complement(3246..3509)
                     /locus_tag="VIF63_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEY8238157.1"
                     /translation="MIDYPDILVLGRDTCEDTIRARDRLRELGIPYVYRNVELDPVAD
                     AWNRSFNDGRRVTPVILVGESDNPTIVLREPSNEDLEAAIAAG"
     gene            complement(3506..4366)
                     /locus_tag="VIF63_01875"
     CDS             complement(3506..4366)
                     /locus_tag="VIF63_01875"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY8238158.1"
                     /translation="MSLYKLRRGLPDLREPAMVVALDGWVDAGSAATNAATTLAQGAR
                     VIATFDSDAIFDYRARRPTLQIVHGKPRSLEWPELTIRATKVADTDVLIFAGAEPDYR
                     WHELATDVVELAGRLGVAQWISLGAIPAAVPHTRPVPILGTESKTGLLKGDIQPGPEG
                     ILRVPAAAISVFDISVTKAGIPAVGYFAQIPHYVSGPYPQASIELLRTLGKHLDLELD
                     VGRLPEEARLMRVRLDAAAAREETTRTYVERLETLVDEARLPSGDDLISEIERFLREG
                     GNQGGSGRPN"
     gene            complement(4363..4740)
                     /locus_tag="VIF63_01880"
     CDS             complement(4363..4740)
                     /locus_tag="VIF63_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8238159.1"
                     /translation="MSSFANLADVQLLKVWGETVKARRVQGERITLAIVELAPNAVVP
                     EHRHANEQLGMVIRGQMHFTVDGETRDLGPGGTWRILGDRPHDVVAGPEGAVVIDVFS
                     PIRSDWDEKVVVDPQPQPRWPEA"
     gene            complement(4737..5243)
                     /locus_tag="VIF63_01885"
     CDS             complement(4737..5243)
                     /locus_tag="VIF63_01885"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8238160.1"
                     /translation="MQTIDIRNLFDYLYWVRDRVTAKAAELDNQAFLDAPTLGRRDLR
                     GTLAHELDVEHSWRPKLQGEPHDNWGPLAEIRAEEFPTLEALMNRWRFDEAEVRDWLA
                     GLSDEDLAAPVTVNGLEGHSLETYLLHVVEHGITEFTMASAILGETGHETGELGYLKY
                     LDDRQEAR"
     gene            5406..>5634
                     /locus_tag="VIF63_01890"
     CDS             5406..>5634
                     /locus_tag="VIF63_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238161.1"
                     /translation="MSTSAPQPKARPKPSPRRLVGAAVLDMVLPGLGHLSIGRRRTAL
                     LFFIPVAVLAAGLIWLYLTGGFTAIVAFAVTP"
BASE COUNT          780 a         2080 c         1884 g          890 t
ORIGIN      
        1 ctcgagctcg cgggtatcga cgccgtgacg cgccgtgctc gggttgccga gctcctcgaa
       61 acggtcggcc tggaggccgc cgcggcgcag gtcccgtcgc agctgtcggg cggcatgcgc
      121 cagcgggcgg ccctggcgcg cacgctcgcc ctggagccgc aggtgctcct cctcgacgag
      181 ccgttcagcg cgctcgacga gctgacccgc gagcgcctca acctggagct gctcgcgatc
      241 accgcccgca cgccaacgac catcctgctc gtgacccact cggtgcagga ggcgatcttc
      301 ctcgccgatc gcgtcgtcgt cctgtccgag cggcccggcc gcgtcgctgc cgacgtgccc
      361 gtcgacctgg cgcgtccacg cgccctcgcc gatctcgacg ccgcggtcgt gaccgagacg
      421 gcgcgcctcg tccgcgcgca cctcggcgag gccgccgcat gacgcccgga tccgccccgt
      481 gaccacgctt gccgcgaccc ggccaaccac gacccggatc ccgggcgcca tcacggcggc
      541 ccttgcgctg gccgccttcc tcggcctctg gaaggggatc gtgctcgtca gcgggttccc
      601 gccgttcatc ctgccgccgc cggagcaggt cgccgagaga ttcgtcggcg cctgggctga
      661 cggcacgatc ggaccgcact tcgtccgaac gctcggcgag gtgctcgtgg gcttcggcct
      721 cggggcgtgg tcggcactct tcgtcggcta cgccctcgcc cggctgccgc tcgccgagcg
      781 cctcctgtcg ccatacctcg tggccgccca ggccatcccg atcctcgtcc tcgcgccgct
      841 cctggccctg tggttcggga ccgggctcac ggccaagctg gtgatctgcg cgctgatcgt
      901 cttcttcccg gtcgcgatca cgacgatggt tggcgttcgc ggggtcgacg cccgactgct
      961 cgagctcgcc cgcagcctcc gcgcgacccg ctggcaggtc atcaggaccg tcgagatccc
     1021 agcggccctg ccacagatct tcggcggcct gcgcgtcggc gtgaccctcg ccgtcgtcgg
     1081 ggcgatcgtc ggggagtgga ccggcgccga tcacggcctc ggcctcctga tgaacctcgc
     1141 ccgcgggtcg ctcttcgata ccccgttgct gttcgcgacc ctcgtgaccg tcgccctgct
     1201 cggggtcgtg ctgtacggcc tcgttgtcct catcgaacgc cggctggtcg gacccagatg
     1261 aaccaagccc gaaaggtgga gaaccgcatg tcccgtcgcg ccctctcgct tctcgccgcc
     1321 gccacgctcc tcgttgtcgc ctgcggcccg agggccgagc cctccacgtg cagcgacgcg
     1381 ctgccaaccg ccggcggctg cggattccag ccaaccccgg acgtcacggg cggcccgcct
     1441 gtcgccctga aggtcgggct cggctacatc ccgagcgtgc agttcgcgcc gttctactac
     1501 gcccagcagg ccggctacta caccgaagca ggcctcgagg tcacgttcga gaacaagatc
     1561 gaccccgacc tcgtcaccct ggtcggccag ggcgcgatcg acatcggcgt cgccgatggc
     1621 acgagcgtga tcccggcggt cagccagggc atcccggtgc ggtacatcgc gacgatctat
     1681 ggcacctacc cctcgatcgt ctttgcgaag gcctcgtccg ggattacgaa ggcggcggac
     1741 ctcgagggca agaagatcgg catcccgggc aagtacggct cgtcctggat catgctccag
     1801 gcgctcctcg gctcggccga cctgaagccg gacgacgccg cgatcgtcga ataccccgac
     1861 ttcggccagg gcgtcgcggt ccaggcgggc gccgtggacg cggcgaccgg cttcgccaac
     1921 aacgagcccg tccagctcga gctcaccggg acgaaggccg tgatcctgca cgtcgacgac
     1981 gttgtggccc tgcccggccc cgggctgatc tcgggcaacg atgcgctcgg cgaggagaag
     2041 gccgaagcga ttgccacgtt cgtctcggtc acgctccggg cgatgaccga gatcgcggcc
     2101 aacccggcgc tggggctcga tgcggcgatc gccgcggtgc ccgagcttgc gacgagtcgg
     2161 gagacccagg cggcgatcct ggacgccacg atcgcgagct ggtcgccggc cggcgggggt
     2221 gccgacttcg gcacgatcga tcgcgccggc tgggaggcct cgctcgccta cctgacgacg
     2281 ctcggtctgg tgcccaaccc ggttgtcgtc gacgacctga tccgcgacga cctcctgccg
     2341 tcgggctgac cgcggctcga cccttcgaag ggacatactc cgagcatggc catgcgcaag
     2401 ttcctcgccg gcgcgtcgga ccggccgttc ccgaccgacg cccacgacac cgagaccgtt
     2461 cgccgcatcg ccggccagct cgacgcgctg ccggtcgatc gcgcgcggtt cctcgcggcc
     2521 ttcgcgtaca tcctgacccg cgcggccgcg gcggatctcg agatcagcgc ggtcgagtcg
     2581 gaggcgatcg agcggctcgt cgccgagcac ggcggactgc ccgacgcgca ggccgtgctc
     2641 gtcgcccaga tcgcgcgcaa ccaggcgctc ctgtacggcg gcaccgagga ctacctcgtg
     2701 acccgccagt tccgcgatct cgcgtcggcc gaggaccggc tcgcgctcct gcgctgctgc
     2761 tacctcgtcg gcgcggccga cgacacgatc acggccgagg agtcgaacct gctccagcag
     2821 atcgccaggg agctcgacat cgaccgcgtg gcggtcaacg ccatccgcac cgagttcgcg
     2881 ccgaagctcg ccgcgatgca gcggatgctc cacctgcgcg aggcggagta ggcgctcacg
     2941 cctccgactc ctggcaggcg ccccaatgat cagaggaaga tcatggggcg atcatgtttg
     3001 gcggtttccg acccatttcg gcccgacggg atgcgcggac gcggctccgc ttgtcgatgg
     3061 tgagcgcggc cccgatcagt gaacgacgat ttcgtgcctc ggacccacac cggcgggccg
     3121 gcggtggggc gaaatgcctc tgcaaacccc gagaaagatc accgtgcgaa catcgatagg
     3181 cagttcgcgg ccaacggcca cgatccacag ctggacccgc gaccgatgga ctcagccgtc
     3241 ggcgatcagc ccgccgcgat ggccgcttcg aggtcctcgt tcgagggttc gcggagcacg
     3301 atggtcgggt tgtccgattc gccgacgagg atgaccggcg tcacgcgccg gccgtcgttg
     3361 aacgagcggt tccaggcgtc ggccacgggg tccagctcga cgttgcggta gacgtacgga
     3421 ataccgagct cgcgcagccg atcgcgggcg cggatcgtgt cctcgcaggt gtcccggccg
     3481 agcaccagga tgtccgggta gtcgatcaat tcggcctccc gctgccaccc tggttgccgc
     3541 cctcgcgcag gaagcgctcg atctcgctga tgaggtcgtc gcccgacggc agccgcgcct
     3601 cgtcgaccag cgtctccagc cgttcgacgt aggtccgcgt cgtctcctcg cgcgccgcag
     3661 ccgcgtcgag gcggacccgc atgagccgtg cttcctcggg cagccgcccg acgtcgagct
     3721 cgaggtcgag gtgcttgccg agcgtgcgca ggagctcgat cgacgcctgc gggtacgggc
     3781 ccgagacgta gtgcgggatt tgcgcgaaat agcccacggc ggggatgccc gccttggtga
     3841 ccgagatgtc gaacaccgag atcgccgccg cggggacgcg caggatgccc tccgggcccg
     3901 gctggatgtc gcccttgagc aggccggtct tcgattccgt gccgaggatg ggcacgggcc
     3961 gggtgtgggg cacggccgcc gggatcgcgc cgaggctgat ccactgggcg acgcccagcc
     4021 gcccggcgag ctcgaccaca tcggtcgcca gctcgtgcca gcggtagtcc ggttctgcgc
     4081 cggcgaagat gaggacgtcg gtgtcggcga ccttcgtggc ccggatcgtc agctccggcc
     4141 attcgaggct gcgcggcttc ccgtggacga tctggagcgt cgggcgcctc gcgcgatagt
     4201 cgaagatcgc gtccgagtcg aaggtcgcga taacgcgggc gccctgggcg agcgtcgtcg
     4261 ccgcgttcgt cgcggcgctg cctgcgtcga cccagccgtc gagcgcgacg accatcgccg
     4321 gctccctgag gtccggcagg ccgcgccgca gcttgtacag gctcacgcct ccggccacct
     4381 cggctgcggc tgcggatcga ccacgacctt ttcgtcccag tcggagcgga tcgggctgaa
     4441 gacgtcgatc acgaccgcgc cctcgggccc cgcgacgacg tcgtgcggtc ggtcgccgag
     4501 gatgcgccac gtcccgccgg ggccgaggtc gcgcgtttcg ccgtcgaccg taaagtgcat
     4561 ctggccgcga atgaccatgc ccagctgctc gttcgcgtgg cggtgctcgg gcacgacggc
     4621 gttcggcgcg agctcgacga tcgcgagcgt gatgcgctcg ccctggaccc gtcgcgcctt
     4681 gacggtctcg ccccagacct tgaggagctg cacgtctgcg aggttggcga agctgctcat
     4741 cgggcctcct gcctgtcgtc gagatacttg aggtagccga gctcgccggt ctcgtggccg
     4801 gtctcgccga ggatcgccga ggccatcgtg aactcggtga tgccgtgctc cacgacgtgc
     4861 aggaggtatg tctcgagcga gtggccctcg agcccgttca ccgtcacggg cgccgcgaga
     4921 tcctcatccg agaggccggc gagccagtcg cggacctccg cctcgtcgaa gcgccaccga
     4981 ttcatcagcg cttcgagggt cgggaactcc tcggcccgaa tctcggcgag cggaccccag
     5041 ttgtcatgcg gctcgccctg gagcttcggc cgccacgagt gctcgacgtc gagctcatgg
     5101 gcgagcgttc cgcgcaggtc gcgccgacca agcgttggcg catcgaggaa cgcctggttg
     5161 tcgagctccg ccgccttcgc ggtcacgcgg tcgcggaccc agtagaggta gtcgaacagg
     5221 ttgcggatgt cgatcgtctg catccgccaa tggtgccctg accggccgcg gcacgcagcc
     5281 gacctcacca gaccgcacga aaccgcccag aaccgccctc aaccgaccct ctgtcaacac
     5341 tcccatacca aaccgttact tccgtaccac taacgagtcg tcaaatccgt tgcatagttg
     5401 ccgccttgtc cacatccgcg ccccaaccca aagcccgacc gaagccgtcg ccacgccggc
     5461 tcgtgggcgc cgccgtcctt gacatggtct tgcccggcct gggccacctg tcgatcggcc
     5521 gtcgccgcac ggcattgctc ttcttcatcc ccgtcgccgt cctcgccgcg gggctgatct
     5581 ggctgtacct gaccggcggc ttcaccgcga tcgtcgcctt cgcggtgacg cccg
//
LOCUS       DATBSW010000069         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_745551, whole genome shotgun sequence.
ACCESSION   DATBSW010000069 DATBSW010000000
VERSION     DATBSW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745551"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VIF63_01895"
     CDS             <1..407
                     /locus_tag="VIF63_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY8238162.1"
                     /translation="STTIVSLLSPNSPLYIVMYFLLTVGFTYFYTAFTFKPDETADQL
                     RKNGGFIPGIRPGRPTQEYLGRVVSRITLAGSLFLGTVAVAPVLAGVIDPKLGGLAIG
                     GTGLLIVVSVVVETMKQIEAQLMMRNYEGFIR"
     gene            547..1215
                     /locus_tag="VIF63_01900"
     CDS             547..1215
                     /locus_tag="VIF63_01900"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY8238163.1"
                     /translation="MTVAILLGAPGAGKGTQAPLLAEKLGVPVLASGDLLRAEVAGGS
                     DIGREVDGIMRSGELVPDDVMARLFLARLGRPDAADGAILDGFPRTRRQAEALDEALN
                     ASGRPVDRALLIEVPIEDLIGRFAGRVICKAGGHVYNEWFKPPRVWGVCDIDGSELVH
                     RADDAEATVRARMEQQLGPLHDVADYYRTRGILTPVDGLQPIEDVAAALLAALGWQTA
                     PQAQ"
     gene            1212..1988
                     /gene="map"
                     /locus_tag="VIF63_01905"
     CDS             1212..1988
                     /gene="map"
                     /locus_tag="VIF63_01905"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8238164.1"
                     /translation="MITRKSAREIEKMRDAGRIVAEVLALMESELKPGVTTRHLDTLA
                     ERHIRSSGAVPSFVGYLGGGRYGKGPKAFPASTCISIDNEVVHGIPGERTIKAGSLVK
                     VDVGAILDGWHGDGARTFAVGDVAPEARALMDATRLALMAGIRAAQPGARIGDISAAI
                     EDVGVAGGYGILRPFVGHGIGTDMHQDPQVPNYRTGSRGVELHPGICLAIEPMFTLGS
                     GDVYVEADGWTVSTADDTLAAHFEHTIAITPNGPEILTQA"
     gene            2243..3211
                     /locus_tag="VIF63_01910"
     CDS             2243..3211
                     /locus_tag="VIF63_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8238165.1"
                     /translation="MVYVPDRESVVGRTLLESRVVHIEDVLADRELTTGTVLDTVRQA
                     GGIRTDLGVPIRSDGQLIGVIAAARNDVRPFSERELDLVQQFADQAAVAINLARLFET
                     IERQRTELARYAPQAAELLSTAEGEQLLAGHRREITALFADIRGFTAFAEKADPEEVL
                     GVLRQYHTTVGELAIANRGTVEHFAGDGLMVFFNDPTPIDDHQLAAVTTACAMRDQFA
                     TLSAAWRKRGYALGLGIGIATGYATVGRIGFEGRYDYGAIGTSVILASRLSSAAKAGQ
                     ILISQRVMAAVEDSVETDEVPDLELKGFSSQITAFAVRTCFGRSAA"
     gene            3325..3543
                     /gene="infA"
                     /locus_tag="VIF63_01915"
     CDS             3325..3543
                     /gene="infA"
                     /locus_tag="VIF63_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY8238166.1"
                     /translation="MAKRDAIEVEGTVVEPLPNTMFAVELENGHRVLAHISGKLRMNF
                     IRILPGDRVRVELSPYGLTRGRITYRLK"
BASE COUNT          558 a         1228 c         1189 g          590 t
ORIGIN      
        1 tctcgacgac catcgtcagc ctgctcagcc ccaacagccc gctctatatc gtcatgtact
       61 tcctgctcac cgtcggcttc acgtacttct acacggcctt cacgttcaag cccgacgaga
      121 cagccgacca gctgcgcaag aacggcggct tcatcccggg catccgcccg ggccggccga
      181 cgcaggaata ccttggtcgg gtcgtcagcc gaatcaccct cgcgggctcg ctcttcctcg
      241 gcaccgtcgc ggtcgcgccg gtcctcgccg gcgtgatcga cccgaagctc ggtggcctcg
      301 cgatcggcgg cacgggcctg ctgatcgtcg tttcggtcgt cgtcgagacg atgaagcaga
      361 tcgaggccca attgatgatg cgcaactacg aaggtttcat ccgctaatgg tcacgccgtc
      421 ggtgacggcc cgtgccggcg ttgcctcctc cgctcgcgca cgcacgcaca ttccaagtgc
      481 gctcgtgcac gtgctcgtcg gcgccttggc ctgggccacc cccaacggca tgaccggcac
      541 gtcgacatga cggtagccat tcttcttggc gcacccggtg ccggcaaggg cacccaggcg
      601 ccactgctcg cggagaagct cggggtgccc gtgctcgctt cgggcgacct gctccgggcg
      661 gaggtcgcgg gcggcagcga catcgggcgt gaggtcgatg ggatcatgcg ctcgggcgag
      721 ctcgtgcccg acgacgtgat ggcccgcctg ttcctcgccc gtctcggccg cccggacgcc
      781 gccgacggtg cgatcctcga cgggttcccg cgcacccgcc gccaggccga ggcactggac
      841 gaggcgctca acgcatccgg gcgcccggtc gatcgtgcgc tgctgatcga ggtccccatc
      901 gaggacctga tcggccgctt tgccggccgc gtcatctgca aggccggcgg gcacgtctac
      961 aacgagtggt tcaagccgcc tcgcgtgtgg ggcgtgtgtg acatcgacgg ctccgagctc
     1021 gtgcatcgcg ccgacgacgc cgaggcgacc gtgcgcgccc gaatggaaca gcagctcggc
     1081 ccgctccacg atgtcgcgga ctactaccgg acgcggggca tcctgacgcc cgtcgacggc
     1141 ctccaaccga tcgaggacgt cgcggctgcc ctgctcgcag cgctcggctg gcagaccgcc
     1201 ccgcaggcgc aatgatcacg aggaaatccg cgcgcgagat cgagaagatg cgagacgccg
     1261 ggcggatcgt cgccgaagtg cttgcgttga tggagtccga gctcaagccg ggcgtcacga
     1321 cgcgccacct cgataccctc gccgagcgcc acatccgcag ctccggggcc gttccctcat
     1381 tcgtcggcta tctcggcggc ggacgctacg gcaaggggcc caaggcgttc cccgccagca
     1441 cctgcatctc gatcgacaac gaggtcgtcc acggcatccc cggcgagcgc acgatcaagg
     1501 cgggctcgct ggtcaaggtc gatgtcgggg cgatcctcga cggctggcac ggcgacggcg
     1561 cccgcacatt cgcggtcggt gacgtcgcgc ccgaggcgcg ggccctcatg gatgccaccc
     1621 ggctggcgtt gatggccggt atccgcgctg cccagccggg tgcccgtatc ggcgacatct
     1681 cggcggcgat cgaggacgtc ggcgtggccg gcggctacgg catcctgcgg ccgttcgtcg
     1741 ggcacggcat cggcaccgac atgcaccagg atccgcaggt cccgaactac cgcaccggct
     1801 cccgcggcgt cgagctccac cccggcatct gcctcgcgat cgagccgatg ttcacgctcg
     1861 gctcgggcga cgtctacgtg gaagccgacg gctggaccgt gtcgacggcc gacgacaccc
     1921 tggccgccca cttcgagcac acgatcgcga tcaccccgaa cggcccagag atcctgaccc
     1981 aggcttgatc cgtacgttgc gtgaccgttc gatcccgtcg ggaggacagg ccgaagcagc
     2041 cgctctggcg gccctcggtc gcacgatggc ttcgatcgcg cgatcacgat tcgacctgga
     2101 tgaggtcctt caggcggtcg tcgaagaggc cgctcaactg tgcgacgccg actccgcaaa
     2161 cattgcggtg cgcgacggtg atgccttccg gatgcgtgcg ttcactggct tctcgcccga
     2221 gttcgaggag ctcgtgcgga gcatggtgta cgtaccggat cgcgagtcgg tcgttggacg
     2281 gacgctcctc gaatcccggg tcgtccacat cgaggacgtt ctcgccgacc gggagctcac
     2341 gaccggcacg gttctggaca ccgttcgaca ggcgggcggc atcagaaccg atctcggcgt
     2401 gcccatccgt tccgacggcc agctgatcgg cgtgatcgct gccgcacgga atgatgtccg
     2461 cccattctcg gaacgggagc tcgacctcgt ccagcaattc gccgaccagg cagccgtcgc
     2521 gatcaacctg gcccggctgt tcgagacgat cgagcgccag cggacggagc tcgcgcgata
     2581 cgcgcctcag gcggccgagc tgttgtcgac tgccgagggt gagcagctgc tggccgggca
     2641 ccgacgggag atcacggcgc tgtttgccga catccgtggc tttacggcct ttgccgagaa
     2701 ggccgatccg gaggaggtgc tgggcgtgct gcggcagtac cacacgaccg tcggtgagct
     2761 tgccattgcc aaccgcggca ccgtcgagca cttcgccggc gacggcttga tggtgttctt
     2821 caatgatccg acaccgatcg atgaccatca gcttgcggcg gtcacgaccg cgtgcgccat
     2881 gcgggatcag tttgcaacgc tgtccgcagc gtggcggaag cgcggctatg cgctgggcct
     2941 gggaatcggg atcgccacgg gctacgcgac ggtcgggcgg atcggctttg agggccgcta
     3001 cgactacggg gcgatcggaa cgtcggtgat cctcgcctcg cgtctgtcgt cggcggcgaa
     3061 ggccggtcag atcctgatca gccaacgagt gatggccgcg gtcgaggact ccgtcgaaac
     3121 cgacgaggtg cccgacctgg agctgaaggg cttcagcagc cagatcacgg cctttgccgt
     3181 caggacctgc ttcggccgct cggcggcgtg agcacgcgct tgcccgccgt tgccacggat
     3241 ggtgacttgc tgatatgctg cgctttcgtg tgtcggcctc cgttgggccg gcattccgtg
     3301 tgagaaaggg acccagcacc gcccgtggcg aaacgagatg cgatcgaggt cgaaggcacg
     3361 gtcgtcgaac cattgccgaa caccatgttc gccgtcgagc tggagaacgg gcacagggtg
     3421 ctggcgcata tctccggaaa gcttcggatg aacttcatcc ggatcctgcc cggcgaccgc
     3481 gtgcgagtag agctttcgcc ctacggcctg acgcgcggcc ggatcaccta ccgcctgaag
     3541 taattgccgt ccgacgcgga cggtc
//
LOCUS       DATBSW010000070         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_887049, whole genome shotgun sequence.
ACCESSION   DATBSW010000070 DATBSW010000000
VERSION     DATBSW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887049"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VIF63_01920"
     CDS             complement(<1..738)
                     /locus_tag="VIF63_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8238167.1"
                     /translation="MTLDVRQEFLVQPVDASWLQCNIECQEACPVGTNCRGYLNLAAE
                     GKFEEGYILSREPNPVAAMCSYVCSAPCERACRRGDIDRPLAIRAMKRFLVEWHEASG
                     IPDVMPHIEARPEKVAVVGAGPAGIAVARELAMKGFGVDVYDSLPFGGGTMLIGVPAF
                     RLPREAIEMDIRLVERLGVTFHYNTNIGVDIAFDDLQAKFDAVAITAGAMNAVALDVP
                     GGDLDGVQYGVDFMKKANLGEPIEVGKD"
     gene            970..2034
                     /locus_tag="VIF63_01925"
     CDS             970..2034
                     /locus_tag="VIF63_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238168.1"
                     /translation="MSPSELAFLALGLLLGAVLVAASLVAFGNRLGRREIRVTVTRDA
                     VPRRSGTLSQDAFLTSAAEPAPGGPADRRGPGRADAGQRPVATPWQPEVRPAPAPERA
                     PMFQPNRTIVPSAPGAVGVAVGVAIMPEVDRELDDMRHESTPDDLYRQPALGTPLERM
                     LRGEHLAMVEVVDAVAGEDSHQRRTWEVLLGTLTAAMADIAVAESVIDFPMGTAFWDS
                     FTVEQCRRVVAALGSMGYHYDGRSGWAGSRVPAYRDLTVALADIGVEPRRVRAWPSQA
                     EIGVLFAGARPAPEELLPAAGPDYTTADMQALLGARADELADLWLAWDKVRPLLLDDQ
                     PAAITADSATEDMSEAPTEA"
     gene            complement(2522..3319)
                     /locus_tag="VIF63_01930"
     CDS             complement(2522..3319)
                     /locus_tag="VIF63_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8238169.1"
                     /translation="MTEQDGAPAHEAHAWRPERFMVIAAHPDDADFGPAATAASWIDQ
                     GSVGWLVCCTSGDAGAEDPDTDPLELAALREREQRAAAEVVGYAGVTFLHMPDGALAN
                     DLILREHLVREIRTFRPDAVLATDPENAFPRDGGVNHTDHRAAGWAAADAVYPAARNP
                     MAFPALARGGLAKHNVRRLYFFWTHRANAWVDITSTVDRKLGALRCHASQIKDFAEVE
                     KWIGEWSGIQGREIGVANADGFYCVVIDDDEDAAPEPAEAAEAGTTA"
     gene            complement(3324..>3587)
                     /locus_tag="VIF63_01935"
     CDS             complement(3324..>3587)
                     /locus_tag="VIF63_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY8238170.1"
                     /translation="AASHARFREKFDLPFTLLTDPNREVIERYGAWGEKTALGRTYMG
                     IVRSTFLIDPDGRIARAWPKVKADGHPAEVLAALGEERAARAS"
BASE COUNT          500 a         1303 c         1243 g          541 t
ORIGIN      
        1 gtccttgccg acttcgatcg gttcgccgag gttcgccttc ttcatgaagt cgacgccgta
       61 ctggacgccg tcgaggtcgc cgccgggcac gtcgagcgcg accgcgttca tcgcgccggc
      121 agtgatcgcc acggcatcga acttggcctg gaggtcgtcg aacgcgatgt cgacgccgat
      181 gttcgtgttg tagtggaacg tgaccccgag ccgctccacg aggcggatgt ccatctcgat
      241 ggcctcgcgc gggaggcgga acgcgggcac gccgatgagc atcgtcccgc cgccgaacgg
      301 caggctgtcg tagacgtcga cgccgaagcc cttcatggcc agctcgcgcg cgacggcgat
      361 cccggccggg cccgcgccga cgaccgcgac cttctcgggc cgcgcctcga tgtggggcat
      421 gacgtcgggg atgccggacg cctcgtgcca ctcgacgagg aagcgcttca tggcccggat
      481 cgcgagcggg cgatcgatgt cgccacgccg gcaggcgcgc tcgcacgggg cggagcagac
      541 gtaggagcac atcgccgcga ccgggttcgg ctcgcgcgag aggatgtagc cctcttcgaa
      601 cttgccctcg gccgcgaggt tcagatagcc gcggcagttc gtcccgaccg ggcaggcttc
      661 ctggcactcg atgttgcact gcagccagga cgcgtcgacc ggctggacga ggaactcctg
      721 gcggacgtcg agggtcaagg cgcggctgga cctcaggtgc gacgacgggg gccggcgaat
      781 cccggtgcgg ccggtgccgt caatctacca tccgccccct tcccacgctc gcccgccgtt
      841 cgcggtctgc ttgccggcgc gctcggtcga ccgtggccca tccaagttga cgcggccatg
      901 gggcgcagct accctccgga ccagcgacgg cgccccgaga ggcgttggac ccgaggttcc
      961 tgaccccgcg tgagcccaag cgagctggcc ttcctggcgc tcggcctcct cctcggagcc
     1021 gtcctcgttg ccgcatccct cgtggccttt ggcaaccgcc tcggccgccg cgagatccgg
     1081 gtcacggtga cccgagatgc cgttccccgc cgttcaggaa ccctgtccca ggacgccttc
     1141 ctgaccagcg cagccgagcc cgcgccgggc ggtccggcgg accgccgagg cccgggtcgg
     1201 gccgacgccg ggcaacgacc cgtggccacg ccgtggcagc ccgaggtccg gcccgcgccg
     1261 gcacccgagc gggcgcccat gttccagccc aatcgaacga tcgttccatc tgcgcccggc
     1321 gcggtcggcg tcgcggtcgg cgtcgcgatc atgcccgagg tcgatcgcga gctcgacgac
     1381 atgcgccacg agtcgacgcc cgacgatctg taccgccagc cggcgctcgg caccccgctc
     1441 gagcgaatgc tccgcggcga gcacctcgcg atggtcgagg tcgtcgacgc cgtggccggc
     1501 gaggacagcc atcagcgccg cacctgggag gtgctgctcg gcacgctgac cgccgcgatg
     1561 gccgacatcg ccgtcgccga gtcggtgatc gacttcccga tgggcaccgc gttctgggac
     1621 agcttcaccg tcgagcagtg ccggcgcgtg gtcgccgccc tcggctcgat gggttaccac
     1681 tacgacggcc gctccggctg ggccggcagc cgcgtgccgg cctaccgcga cctgaccgtc
     1741 gccctcgccg acatcggcgt cgagccgcgg cgagtccgcg cctggccgag ccaggccgag
     1801 atcggggttc tgttcgccgg agcccgaccc gcccccgagg agctgctccc cgctgcgggg
     1861 cctgactaca ccaccgccga catgcaggcg ctgctcgggg ctcgtgccga cgagctcgcg
     1921 gacctctggc ttgcctggga caaggtccgc ccgctgctcc tcgacgacca gccggccgcg
     1981 atcactgccg actccgcaac cgaggacatg tccgaggcac ccaccgaagc ctgagcctcg
     2041 agtgggtcct gagcctccac cgagtcctcc ctccacctag tcctcagcct caaccgaggc
     2101 ccgagcctcg agctcgccag ccgcggggcc gagaattcaa gccccccgca cctggcgccg
     2161 gccgccagtc cgctgatctg cttccggatg ttcatgtggt gatcagattc gggcgttcgc
     2221 aggtcaattt gcggctaacg cgcgccgatc ggcggcactg ccgaccggag aacgccgcga
     2281 ttcgtgctca atcgagctcg aggtcaagcc gtgaccgggg ttcccggctc cctcgaaggg
     2341 catcgtcgag ggcaaatgcc cttccaagtg ccagaaatga tcaccacgtg aatacggcga
     2401 ggcacttgct cctcgctcgg ctcatgcgcg caggaccttg agcacgctcg gctcctggct
     2461 tgcacggtcg agcccagttc ggccggttcg gcggcttcgg ccgggtcggg cggctcctgc
     2521 gctaggcggt ggtgcccgct tcggctgcct cggcgggctc cggggccgca tcctcgtcgt
     2581 cgtcgatgac gacgcagtag aagccatccg cgttcgcaac cccgatttcg cgtccctgga
     2641 ttcccgacca ctcgccgatc catttctcga cctcggcgaa gtccttgatc tggctcgcgt
     2701 ggcagcgcag cgcgccgagc ttgcgatcga cggtcgacgt gatgtcgacc caggcgttcg
     2761 cacggtgggt ccagaagaag tacaggcggc ggacgttgtg cttcgcgagc ccgccgcggg
     2821 caagcgccgg aaaggccatc gggttgcggg ccgccgggta gacggcatcc gcggccgccc
     2881 agccggcggc acggtggtcg gtgtgattca cgccgccgtc gcgcgggaag gcgttttcgg
     2941 gatcggtcgc gaggacggcg tccgggcgga acgtgcgaat ctcgcgcacg aggtgctcgc
     3001 gcaggatcag gtcgttggcg agcgcgccgt cgggcatgtg caggaaggtc acgcccgcgt
     3061 agccgacgac ctcggcagcg gcgcgctgct cgcgctcgcg aagggcggcg agctccaggg
     3121 gatcagtgtc aggatcctcg gcgcccgcgt cgccgctcgt gcagcacacg agccagccga
     3181 ccgagccctg gtcgatccac gaagcggcgg tcgcagccgg cccgaagtcg gcgtcgtcgg
     3241 ggtgggcggc aatgaccatg aaccgctccg gacgccaggc atgggcctcg tgcgcagggg
     3301 ccccgtcctg ttccgtcatc gcatcacgag gcgcgagcgg cgcgctcctc gcccagcgcg
     3361 gcgagcacct cggccgggtg gccgtccgcc ttgaccttcg gccacgcgcg ggcgatgcgc
     3421 ccgtcgggat cgatgaggaa tgtcgagcgg acgatgccca tgtacgtccg acccagagcg
     3481 gtcttctcgc cccacgcccc gtagcgctcg atgacctcgc gattcgggtc ggtcagcagc
     3541 gtgaacggca ggtcgaactt ctcgcggaag cgggcgtggc ttgccgc
//
LOCUS       DATBSW010000071         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_111114, whole genome shotgun sequence.
ACCESSION   DATBSW010000071 DATBSW010000000
VERSION     DATBSW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111114"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /gene="glmU"
                     /locus_tag="VIF63_01940"
     CDS             <1..1153
                     /gene="glmU"
                     /locus_tag="VIF63_01940"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY8238171.1"
                     /translation="DAVRAALAELPADVAEVLVLSGDVPLVQADQLTAVLEQRRLDDA
                     AITLASVFAADPGELGRVVRSEFGSVERIVEARDATADELETNEINAGVYAFDAAWLR
                     RRIETLVPSKSNGELYLTDLVQLAREDGRIVSAVGFDDDGSLDGINDRSQLAQAEWAL
                     RVRLNEGHMRAGVTMRDPSTVYVDWGVQLAKDVTLEPGVVLRGATSIGEGSVIGPDTT
                     IVDSTIGRDCSVSRSVVEGSTVDDGATIGPYSHLRKGSHVGAGAEIGNFAEIKNSRLG
                     ARVKQHHMSYLGDAEIGAGTNVGAGTITANWDGRIKNRTQIGENVFLGVDTMLVAPVE
                     VGDGAKTGAGAVVTKNVPAGKLAVGVPARIREIRVKPDAPGNPAASDGS"
     gene            1150..2595
                     /locus_tag="VIF63_01945"
     CDS             1150..2595
                     /locus_tag="VIF63_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8238172.1"
                     /translation="MNFPLGEILFLVFLTFLEGFFVAAEIALVSVRRSRIDQLVEEGN
                     QGARRVRKLIDDPGRFLAVAQLGLTLIGFFASAFAAVSLVEGLQDLLLGFGVPQATAE
                     TVALIIVTFILAMFTIVFAELIPKSIALAAPERFALLLSRPIDVLARLLGPVVTVLNG
                     ITRWVARSIGVDMNQEAQISADELRLIVERGGEQGVLEAEEEQMINAVIELGERRVHE
                     VMIPRVAIAALASDATLEQAIDLVVEVGHSRIPVYHDSIDEIVGILYAKDLLPYLKAD
                     AGPRPALRKLLRPPVLVPESMTVDDLLHEFQRRKVHIAIVLDEYGGTAGLVTIEDLLE
                     EIVGEIQDEYDVEEPMVVRLSDHEARVDGRADVDELLELFDLDLELEDAEEYDTVGGL
                     MYHRVGGVPAPGDSIDVDGLRLTVESTDGRRVGKVLVTRRVEKAADDTDSDGTESEPT
                     IATPRRRAADRTPSGRSSSGRGSKRSSTSRG"
BASE COUNT          491 a         1077 c         1074 g          473 t
ORIGIN      
        1 cgacgcggtt cgagccgcgc tcgccgagct gcccgccgac gtcgccgagg tgctcgtgct
       61 ctcgggcgac gtgcccctcg tccaggccga ccagctgacc gcggtcctcg agcagcgccg
      121 gctcgacgat gccgcgatca cgctcgcctc cgtgttcgcc gccgatcctg gcgagctggg
      181 gcgcgtggtc cgcagcgagt tcggttcggt cgagcggatc gtggaggcgc gcgacgcgac
      241 cgccgacgag ctcgagacca acgagatcaa cgccggggtg tacgcgttcg atgcggcatg
      301 gctgcggcgg cgaatcgaga cgctggtgcc ctcgaagtcg aatggcgagc tctacctcac
      361 cgacctcgtc cagctcgccc gcgaggacgg ccggatcgtg agcgcggtgg gcttcgacga
      421 cgacggctcg ctcgacggga tcaacgatcg cagccagctc gcccaggccg agtgggcgct
      481 gcgggtccgg ctgaacgagg gccacatgcg cgccggcgtg accatgcgcg acccatcgac
      541 ggtctatgtc gactggggcg tccagcttgc gaaggacgtg acgctcgagc ccggcgtcgt
      601 cctgcgcggc gcgacctcga tcggagaggg cagcgtcatc ggcccggaca cgacgatcgt
      661 cgactcgacg atcgggcgcg actgcagcgt cagccggagc gtcgtggagg gctcgacggt
      721 cgacgacggc gcgacgatcg ggccgtacag ccacctgcgg aagggcagcc acgtcggtgc
      781 gggtgcggag atcggcaact tcgcggagat caagaactcg cgcctcggtg cgcgggtcaa
      841 gcagcaccac atgagctacc tcggcgacgc cgagatcggg gccggcacga acgtgggcgc
      901 gggcacgatc accgcgaact gggacggcag gatcaagaat cggacccaga tcggggagaa
      961 cgtcttcctc ggcgtcgaca cgatgctcgt tgccccggtc gaggtgggtg atggggcgaa
     1021 gaccggcgcc ggcgccgtcg tcacgaagaa cgtgccggcg ggcaagctcg ccgtcggcgt
     1081 gcccgcgcgg atccgcgaga tccgcgtgaa gcccgacgcg ccgggcaacc ccgcggcatc
     1141 ggacggctcg tgaacttccc gctcggcgag atcctgttcc tcgtcttcct gacgttcctg
     1201 gagggcttct tcgtcgccgc cgagatcgcg ctcgtgtccg tgcgccgcag ccgcatcgac
     1261 cagctggtcg aggagggcaa ccagggggcc aggcgcgtcc ggaagcttat cgacgacccc
     1321 ggccggttcc tcgccgtcgc gcagctcggc ctgacgctca tcggcttctt cgcctcggcc
     1381 ttcgccgcgg tcagcctcgt cgagggcctc caggacctcc tcctcggctt cggcgtcccc
     1441 caggcgaccg ccgagaccgt cgccctgatc atcgtcacgt tcatcctggc aatgttcacg
     1501 atcgtcttcg ccgagctgat cccgaagtcg atcgccctcg ccgcgccgga gcggttcgcg
     1561 ctgctgctct cccgcccgat cgacgtcctc gcccgcctgc tcgggccggt cgtgaccgtg
     1621 ctcaacggca tcacccgatg ggtggctcgg tcgatcggcg tcgacatgaa ccaagaggcg
     1681 cagatcagcg ccgacgagct gcgcctcatc gtggagcgcg gcggtgagca aggcgtgctc
     1741 gaggccgagg aagagcagat gatcaacgcg gtcatcgagc tcggcgagcg ccgcgtccac
     1801 gaggtcatga tcccgcgcgt tgcgatcgcc gccctcgcat cggacgcgac cctcgagcag
     1861 gcaatcgacc tggtcgtcga ggtcgggcac tcgcgcatcc ccgtctacca cgactcgatc
     1921 gacgagatcg tcggcatcct ctacgccaag gacctgctgc cgtatctcaa ggccgacgcc
     1981 ggtccgcggc ctgccctgcg caagctgctg cgcccgccgg tcctcgtgcc cgagtcgatg
     2041 acggtcgacg acctgctcca cgagttccag cggcgcaagg tccacatcgc gatcgtgctc
     2101 gacgagtacg ggggcacggc cggccttgtc acgatcgagg acctgctcga ggagatcgtg
     2161 ggcgagatcc aggacgagta cgacgtcgag gagccgatgg tcgtgcgcct gtcggatcac
     2221 gaggcccggg tcgacggtcg agccgacgtc gacgagctgc tggagctgtt cgacctcgac
     2281 ctcgagctcg aggatgcgga ggaatacgac acggtcggcg ggctgatgta ccaccgcgtc
     2341 ggcggcgtgc cggcgcccgg cgattcgatc gacgtcgacg ggctccgcct gacggtcgaa
     2401 tcgactgatg gccggcgcgt cggcaaggtc ctcgtgacgc gccgcgtgga gaaggccgcg
     2461 gatgacaccg attccgacgg gaccgaatcg gaaccgacca tcgcgacgcc gcgccggcgg
     2521 gcggccgatc gcaccccgag cggccgcagc tcgagtggcc gcggctcgaa gcggtcctcg
     2581 acgagccgag gttgagccag gcgccggtca atcgagcgtc ggtcgcgccc ctcgcgcgcc
     2641 cttgcaagcg ctggaatcgc ctctttccga tgtagtcccg cgccggcgct ctcttcacga
     2701 ggccaagtcc cacgccgtga tcaccggacg atcaaatctt gggtttcgcg tggcatttcg
     2761 gcgcttcggc tgtgatgaac accggtttga cgcgttcggg cgtgctcgaa cgcccatcac
     2821 ccacgctcaa tctggcgctg accgaggtgg gtgggagcct gcgcccgggc aaatgagcgt
     2881 gcaaccccag aacatgatca tgtggcgaac atccgttgtc agatgggcgt ccggagcttc
     2941 tgagaaaccc gctgacctgc ggctaggcca cacccgcgtt cacacggtga acagaatctg
     3001 cccaagatcg ggcgcaaggc gacccacccc gccgcctcgg ggtccaaggc ccgtcgacgg
     3061 cgtgtggttt tcgtgctcgt tgcacagcga acaaggccca ggcaaccggc gatca
//
LOCUS       DATBSW010000072         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_992696, whole genome shotgun sequence.
ACCESSION   DATBSW010000072 DATBSW010000000
VERSION     DATBSW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992696"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..>2605
                     /locus_tag="VIF63_01950"
     CDS             115..>2605
                     /locus_tag="VIF63_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8238173.1"
                     /translation="MSDENRSSNGVPNLRVIGPTGADALRRLASELSGADALQPVLEE
                     VLDNSERLFHADRAGLWLWHETKEHPLELVAKRRFPEAIGRRVRAATHDSKLAGFEAL
                     RRESVIVYRDATDDTITNEMRDLYAASGIASLCFVPAVFRGAPVALLVLYHNEPYDWT
                     PEEILLAGSFGDTIATAIGNAQLMASVEDLAARLRAIQDLSARLSSIQDVKGIGDTIV
                     AEARQLIAYDTLRVYRVDHDTGWCEPIAFQGVFMGRTDPEPELLRVKVGEGLTGWVAE
                     HAETLVLGNAHVDERSIVVGENSGPESMLLVPMKYEGRVRGIIVVSRLGLDRFGDNDE
                     TTLSIFAGAAAQALVNAERLEQLRTQQLELEHQLVGQRRLMAVNEQLLSTLDPKGVLE
                     MIADSLKTVVTYDSLTIYRVDRERGVRRPVIARDRFAELILGYDAPLGTGLTGWAVDQ
                     REALLANDAHIDPRSIQIPGTPFEAESMVIVPLMGDGEVLGTLNIGRMGGDEAHYTQN
                     EFELTKLFAAQAAIALRNADAHDEVKVQAERDALTGLRNHGSFQRELEAFLAGGPTRQ
                     VAVLMMDLDRFKGFNDRNGHPAGDELLSAVSRSIESCVRQGDRAYRYGGDEFAVILPD
                     VGRPAAEEVARRIRSAIEAIPDETGGAHVTISIGIACHPDDATDKESLVETADQALFL
                     AKGAPFRSSRDQFVAAFDETAMGLLDGLGTEELLDSILGRAARLLGVRTGYVYLGEPD
                     DDHITMRAGIGADAEYVGFRQPIGEGMAGAVYRTGRPLVVDDYDSFEGRSATFAGTVG
                     AGVSVPLTIGGRVVGVLGLASGTKDRTFRPHE"
BASE COUNT          404 a          948 c          889 g          364 t
ORIGIN      
        1 gacctcgagc gactgctgac cgaccgagcg ctacgcgccc cctcgatcgc actgccgcgc
       61 tcgaacgggc gcgagggtcc caacgccacg gtgcggagcc gcggcgggag cgacgtgagc
      121 gacgagaacc gctcctcgaa cggcgtcccg aacctccgtg tgatcggccc gaccggcgcg
      181 gacgccctgc gccgcctcgc ctcggagctg tccggggccg acgccctcca gcccgtcctc
      241 gaggaggtcc tcgacaactc cgagcggctc ttccacgcgg accgcgccgg cctgtggctc
      301 tggcacgaga cgaaagagca tccgctcgag ctcgtcgcga agcgccggtt ccccgaggcg
      361 atcgggcggc gcgtccgcgc cgccacccac gattcgaagc tcgcaggctt cgaggccctc
      421 cgccgcgagt ccgtgatcgt ctatcgcgat gccaccgacg acacgatcac caacgaaatg
      481 cgggacctgt acgcggcgag cggcatcgcc tcgctgtgct tcgtgcccgc ggtgttccgc
      541 ggcgcgccgg ttgcgctcct cgtgctctat cacaacgagc cctacgactg gacgccggag
      601 gagatcctgc tcgccggcag cttcggcgac acgatcgcga cggccatcgg caacgcccag
      661 ctcatggcct cggtcgagga tctcgcggcg cgactccggg cgatccagga cctctccgcc
      721 cgcctgagca gcatccagga cgtcaagggc atcggcgaca cgatcgtcgc cgaagcacga
      781 cagctgatcg catacgacac gctgcgcgtc taccgggtcg atcacgacac gggctggtgc
      841 gagccgatcg cgttccaggg cgtgttcatg ggccgcaccg atcccgagcc cgagctcctc
      901 cgggtcaagg tcggcgaagg cctcaccggc tgggtggcgg agcacgccga gaccctggtc
      961 ctcgggaacg cgcatgtcga cgaacgcagc atcgtcgtgg gcgagaactc gggccccgaa
     1021 tcgatgctcc tcgtgccgat gaagtacgag ggccgcgtgc gcggcatcat cgtcgtctcg
     1081 cgcctcggcc tcgaccgctt cggcgacaac gacgagacca ccctgagcat cttcgccggc
     1141 gccgcagcgc aggcactggt caacgccgag cgcctcgagc agctgcggac ccagcagctc
     1201 gagctcgagc accagctcgt cggccagcgc cggctcatgg ccgtcaacga gcagctcctg
     1261 tcgaccctcg acccgaaggg cgtcctcgag atgatcgcgg actcgctgaa gacggtggtg
     1321 acctacgact cgttgacgat ctaccgggtc gatcgcgagc gcggcgtgcg ccgcccggtc
     1381 atcgcccgcg accggttcgc cgagctgatc ctcggctacg acgcgccact gggcacaggg
     1441 ctcaccggct gggcagtcga ccagcgcgag gcgctcctcg ccaacgacgc ccacatcgac
     1501 ccgcgttcga tccagattcc ggggacgccg ttcgaggccg agtcgatggt catcgttccg
     1561 ctgatgggcg acggcgaggt gctgggcacg ctgaacatcg gtcggatggg cggcgacgag
     1621 gcgcactaca cccagaacga gttcgagctg accaagctct tcgcggcgca ggccgccatc
     1681 gcgctccgca acgccgacgc gcacgacgag gtgaaggtcc aggccgagcg cgacgcgctg
     1741 accgggctgc gcaaccacgg ctcgttccag cgcgagctcg aggcgttcct cgccggcggc
     1801 ccgacgcgcc aggtcgcggt gctgatgatg gatctcgacc ggttcaaggg cttcaacgac
     1861 cgcaacggcc atccggccgg cgacgagctg ctctccgccg tgagccgctc catcgagtcc
     1921 tgcgtccgcc agggcgaccg ggcgtatcgc tacggcggcg acgagttcgc cgtgatcctg
     1981 ccggatgtgg gccggccggc ggccgaggag gtcgcgcgcc gaatccggtc cgcgatcgag
     2041 gcgatccccg acgagaccgg cggcgcgcac gtcacgatca gcatcggcat cgcctgccat
     2101 cccgacgacg ccaccgacaa ggagagcctc gtcgagacgg cggaccaggc gctgttcctg
     2161 gccaagggcg cgccgttccg cagctcccgc gaccagttcg tcgcggcatt cgacgagacc
     2221 gcgatgggcc tgctcgacgg cctcggcacc gaggagctcc tcgactcaat cctcggtcgc
     2281 gccgcccgcc tgctcggtgt gcgcacgggg tacgtctacc tcggtgagcc ggacgacgac
     2341 cacatcacga tgcgcgccgg gatcggcgcc gacgcggagt acgtggggtt tcggcagccg
     2401 atcggcgagg gcatggccgg cgccgtctac cggaccggtc ggccgctcgt cgtcgacgac
     2461 tacgactcgt tcgagggtcg gtcggcgacg ttcgcaggca cggtcggtgc cggggtcagc
     2521 gtgccgctca ccatcggtgg gcgggtcgtc ggcgtgctcg gccttgcgtc gggaacgaag
     2581 gatcggacct tccgtccgca cgaag
//
LOCUS       DATBSW010000073         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1063521, whole genome shotgun sequence.
ACCESSION   DATBSW010000073 DATBSW010000000
VERSION     DATBSW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063521"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VIF63_01955"
     CDS             complement(<1..477)
                     /locus_tag="VIF63_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238174.1"
                     /translation="MTMDDRLPSAPDPWASSEMPARRDGPPYHMTDMIEAEPALAARL
                     LTRLADPAGGAARLAAAVRASAEAAQPILVTGCGTSEHGALATVEILREQMRTAKLPW
                     RVGEAGTVAAVQAFEGSLEDWLGSFGGLVIGVSHEGGTWATNRALATARASGATLAL"
     gene            complement(474..911)
                     /locus_tag="VIF63_01960"
     CDS             complement(474..911)
                     /locus_tag="VIF63_01960"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEY8238175.1"
                     /translation="MSDRARPWRVAVTEASMLPAIEPGDWLVVNPRMAAWPARGAVVV
                     FRDPISDELAIKRVAARPGERVRFAEGYLTLADDEAWLIADASTVVTAAAGYGAPIDS
                     TRFGPVTLDHLVGRVLFRYAPLRRFGRIGPAPARVAAPIVDAS"
     gene            complement(940..1380)
                     /gene="sodN"
                     /locus_tag="VIF63_01965"
     CDS             complement(940..1380)
                     /gene="sodN"
                     /locus_tag="VIF63_01965"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY8238176.1"
                     /translation="MSIERAIHRFLEPRTLVRAHCDLPCGIYDPEQARIEAESCLRIV
                     EKYAANDDAAYRTRAIGIKEQRADLVKHHLDVLWHDYFKPEHLEKVPNLHDLFWQANK
                     QVSKVKGSTDPADAKKLLELIDEVDAAWKATGGLEKTRVSGRPS"
     gene            1535..2623
                     /locus_tag="VIF63_01970"
     CDS             1535..2623
                     /locus_tag="VIF63_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238177.1"
                     /translation="MLTDPVRLALVIAGALLLVPSAPSSGHIAFLIGVGVVLVIATWR
                     GARRATLVLLVVGGICVRLAIIDKVGSDVLTVTEAAIRRMLAAENPYGIGYNESSPPG
                     APFPYGPIALLWYVPAGSFAEILEVGAGIAVTVFLALRGRLIGLAVYAASTLLINSSI
                     DGSNDTTLGLLILAAFVAAERWPLAGAGILGLAVGFKLSALAFVPMFVAWGGVRVGAF
                     VVAASLIAWAPVIANWGIASFLRSVQLAEATHRVPTWSLGRIVTDFTGSKVPFLDNLR
                     YALGGAVVLASLAIPRRTLDTVILTGVAVYVVTLFAGNWASYAYFAGIAPVICWRLDG
                     WLGITSTPVLRWPPRAPQDAPEVEAAQV"
     gene            2633..>3233
                     /locus_tag="VIF63_01975"
     CDS             2633..>3233
                     /locus_tag="VIF63_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238178.1"
                     /translation="MTERGLPIADGAPACPFVAFEDDRDERATSPDHRHRCYAEATPA
                     PRALAHQEAYCLSSAFPVCPTFQDWARREAARARGVAERAEAAALEGSGGADQPQRNP
                     PRNWAAPPPWLGGGRDPSGGLWDDEDADEDGFDDAEPVASDVPRRGRGLSGSYADRVA
                     SDPVEAMPGGLPDESELEPPAPAAASEPAGNLPPRIRHRE"
BASE COUNT          416 a         1142 c         1166 g          509 t
ORIGIN      
        1 gagggcaagc gtcgcgccgg aggcgcgggc ggtggcgagc gcacggttcg tcgcccatgt
       61 cccgccttcg tggctgacgc cgatgacgag gccgccgaag ctgccgagcc agtcctcgag
      121 cgagccctcg aatgcctgca cggcggccac cgtgccagcc tcgccgaccc gccacggcag
      181 cttcgcggtt cgcatctgtt cccggaggat ctcgacggtg gcgagcgcgc cgtgctcgct
      241 ggtgccgcag ccggtgacga ggatgggctg ggctgcctcg gcacttgcgc ggacggcggc
      301 ggcgaggcgc gcagccccgc cggccgggtc ggcgaggcgc gtgaggaggc gtgccgcaag
      361 cgcaggctcc gcctcgatca tgtcggtcat gtggtacggc ggcccgtccc gccgagccgg
      421 catctcgctg ctggcccacg gatccggcgc cgacggcagg cggtcgtcca tcgtcatgac
      481 gcgtccacga taggtgccgc aacgcgtgca ggcgcgggcc cgatgcggcc gaaccggcgc
      541 agcggcgcat agcggaacag gacgcgcccg accaggtggt cgagcgtcac cggcccgaag
      601 cgggtcgagt cgatcggcgc gccatacccg gcagccgcgg tgaccaccgt cgaggcatcc
      661 gcgatgagcc acgcctcgtc gtcggccagg gtgaggtagc cctcggcgaa gcgcactcgc
      721 tcgcccggcc gggccgcgac ccgcttgatc gccagctcgt cggagatcgg gtcacggaac
      781 acgacgaccg cgccccgggc gggccacgcg gccattcgcg ggttcacaac gagccagtcg
      841 cccggctcga tagccggcag catcgacgct tccgtgaccg cgacccgcca cggccgcgcc
      901 cggtcgctca tgctcgcccg agcgtccggc cagcgagtgt cagcttgggc gcccgctaac
      961 ccgcgtcttt tcgaggccgc cggtcgcctt ccacgccgca tcgacctcgt cgatgagctc
     1021 gaggagcttc ttggcgtctg cgggatcggt cgagcccttg accttggaga cctgcttgtt
     1081 ggcctgccag aagaggtcgt gcaggttcgg gaccttctcg aggtgctcgg gcttgaagta
     1141 gtcgtgccag agcacgtcga ggtgatgctt cacgaggtcg gcgcgctgtt ccttgatgcc
     1201 gatggcgcgc gtccggtagg cggcgtcgtc attcgcggcg tatttctcga cgatgcggag
     1261 gcaggactcc gcctcgatcc gagcctgctc cggatcgtag atgccgcacg ggagatcgca
     1321 gtgcgcgcgg accagggtcc gcggctccag gaatcggtgg atcgcgcgtt cgatggacat
     1381 gggagctcct tcggaacgac cgggattgtt gcttcggagt gtcgagacgc tgctccggac
     1441 tgtcaaacgg cccacagggt agcctcggct gagggctgat cgggccgagg gtcggccgat
     1501 atgatcggtc gcgtcgtccc aggagtcggt ccacttgctc accgatcccg tccggctcgc
     1561 gctcgtcatt gccggcgcgc tcctgctggt cccgagcgca ccgagctcgg gccacatcgc
     1621 attcctgatc ggcgttggcg tcgtgctggt gatcgcgacg tggcgcggcg cccgcagggc
     1681 gacgctcgtg ctgctcgtgg tcgggggcat ctgtgtccgc ctcgcgatca tcgacaaggt
     1741 tggctcggac gtgctcaccg tcaccgaggc ggcgatccgg cggatgctgg cggcagaaaa
     1801 cccgtacggc atcggctaca acgagtcgtc gccgcctggc gcgccgttcc cgtacgggcc
     1861 catcgcgctg ctctggtacg tcccggcggg ttctttcgcc gagatcctcg aggtcggggc
     1921 cgggatcgcc gtcacggtgt tcctcgcact ccgcggcagg ctcatcgggc tggcggtgta
     1981 cgccgcctcg accctgctca tcaacagctc gatcgatggc tcgaacgaca cgacgctcgg
     2041 cctgctcatc ctcgcggcgt tcgtcgccgc cgagcgatgg ccccttgccg gggccggcat
     2101 cctcggcctc gcggtcgggt tcaagctgtc cgcgctggcg ttcgtgccga tgttcgttgc
     2161 ctggggcggt gtgcgggtcg gcgccttcgt cgtcgccgcg agcctgatcg cgtgggcgcc
     2221 ggtgatcgcg aattggggca tcgcgagctt cctgcgcagc gtccagctcg cggaggcgac
     2281 acatcgcgtg ccgacgtggt cgctcggccg gatcgttacc gacttcaccg ggtcgaaggt
     2341 ccccttcctg gacaacctgc gctacgccct cggtggcgcg gtcgtcctgg cctcgctggc
     2401 aataccccgg cgcacgctgg acacggtcat cctcacgggc gtcgcggtgt acgtcgtgac
     2461 gctgttcgcc ggtaactggg cgtcctacgc ctacttcgcc gggatcgcgc cggtgatctg
     2521 ctggcgcctg gacggatggc tcggcatcac gtcgactccg gtgctgcgct ggccgccgag
     2581 ggcgccgcag gatgcgcccg aggtcgaagc cgcgcaggta tgatcggcgg agatgaccga
     2641 gcgaggcctg cccatcgccg acggtgctcc tgcctgtccg ttcgtcgcct tcgaggacga
     2701 ccgggacgag cgcgcgacga gccccgatca tcgccatcgg tgctacgcgg aggcgacgcc
     2761 cgcgccgcgc gccctcgccc accaggaggc gtactgcctc tcctcggcgt tcccggtctg
     2821 cccgaccttc caggactggg cgcgacgcga ggcagcccga gccaggggcg tcgcggagcg
     2881 cgctgaggcc gccgccctcg aaggttccgg tggcgccgac cagccccaac gcaatccgcc
     2941 gcggaactgg gccgcaccac cgccgtggct cggcgggggc cgggaccctt cgggcgggct
     3001 ctgggacgac gaagatgcgg acgaggacgg gttcgacgac gccgagccgg tggcgagcga
     3061 cgtcccgcgc cgaggtcgag gcctgtccgg cagctacgcg gaccgcgttg cgtccgatcc
     3121 ggtcgaggcc atgcccgggg gcctgcccga cgaatcggag ctggagccac ccgcgcccgc
     3181 cgcggcgtcc gagccggccg gtaacctgcc gccgcgaatt cgacaccggg agc
//
LOCUS       DATBSW010000074         6013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_852053, whole genome shotgun sequence.
ACCESSION   DATBSW010000074 DATBSW010000000
VERSION     DATBSW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852053"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VIF63_01980"
     CDS             <1..303
                     /locus_tag="VIF63_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238179.1"
                     /translation="VVTDCDETRPVEDPYEPHWSIRGCAIAAATTLNITTANVSVAYT
                     APPNTTLTCDPTLHVGCIAAITVTYQYATTTPFVSWIIQPFTMSQTSSMPIERVFP"
     gene            300..1655
                     /locus_tag="VIF63_01985"
     CDS             300..1655
                     /locus_tag="VIF63_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY8238180.1"
                     /translation="MTDRSGAVRGAGVESHPNQRGQAIVVMVGAILLALALVALIVDG
                     GNVLAQQRVAQNGVDATSEAGAVLLAERLAGATAPSGGWDVNIAGRLAQTAAANNVTV
                     EAAYYTDICGIPLRADGTGAIDDDTRVEDLSVALQVGNASHILPGGSATAPDCPNRLV
                     GPVAGVLVIGRKDVAAYVAGAIGFSQFRINTRATAVAGYLQGYCDSSEGHHCAVLPVA
                     IPVNAVTCDGSGDVAGGTEHWAWNQIVTVPLCKSNPGNVGWLDWTPPGGGAGELVCSI
                     VNTNNPSIILPSWMNVPSTGNVNGGGSCADAVTGISYSGVEGALRKYNGQVILVPQFD
                     LTCKAKNNDPDPISSLPAVVTAPNYGCPNAPGGGTGQNIWYRMPSFAFFQMCEPSMAG
                     CAGRQGAYIQGNNSAECDTGNGGTSCLVGKFVDIMSTGTVGAGVGSGTGSKAVGVQLI
                     K"
     gene            complement(1667..2056)
                     /locus_tag="VIF63_01990"
     CDS             complement(1667..2056)
                     /locus_tag="VIF63_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238181.1"
                     /translation="MLLALAFAAAFAACSGAAASPTPAPTARPTPTPIVAPASTADEA
                     AALVIATNPMFTGAMPLTPDVIGASKYWVAAPLPGGGYSIDITIGWGDCPAGCINKHV
                     WTFEVTAAGGVKLISDKGDEVPTDLPA"
     gene            2141..2701
                     /locus_tag="VIF63_01995"
     CDS             2141..2701
                     /locus_tag="VIF63_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8238182.1"
                     /translation="MPTRADAWDLLCEWTKTDALRRHGLAVEAAVAWYGEHKFGIAGD
                     ELEIWRSAGLLHDFDYERYPETHPMRGADELRAHGYREDVVEAILGHGDHTGIARTSL
                     LAKTVYACDEMTGFVIAVTYVRPSRSLDEVEVRSVVKKMKDKGFARQVPRDQLEKGAT
                     ELGVPFEEHVENVIAGLKTIRGELGL"
     gene            complement(2698..3321)
                     /locus_tag="VIF63_02000"
     CDS             complement(2698..3321)
                     /locus_tag="VIF63_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY8238183.1"
                     /translation="MADVLMLGALATSVFGGFRSGFVRRLAGLVFLGVSFVAGAYLRE
                     PAGALLNEFFPKIPPQYADMVGYSVVFSGLLIVFNLFSKTILSRVAVGGVSKATDRVL
                     GAAFGAAESILLFSALIVILHTYTDPNNALSALTDLGVLHDIRLAVDESTIGQLLEKT
                     TVPIVLTVLGPLLPTDIKSIVPMAIPGGIPGFPIPGGVPGLPIPGAP"
     gene            complement(3364..4428)
                     /locus_tag="VIF63_02005"
     CDS             complement(3364..4428)
                     /locus_tag="VIF63_02005"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEY8238184.1"
                     /translation="MDSLGIRFGSPLAGLAVLRNATSRRASSWDRTGGNDDRIHLQPG
                     QEAVLAEMEGAGAVRHIWMTLGSDEPDFLRKVVLRAWWDGEPEPSVNVPIGDFFGVGH
                     GLTTNFASMPLQMSPQDGRGFACWFPMPFAAGARITAQSECETAEVKLYFYVDYELYD
                     ELPSDMARFHAQWRRLNPTDGVDAAAFTNHQLLEEGENASGEGNYVILDARGRGHYVG
                     CNLNVHNLRETAEWNWYGEGDDMIFVDGEPFPPSLHGTGTEDYFGAAWCPTDAFAGPY
                     QGITLPGGPNWSGKASLYRFHVEDPVHFRESIRVTIEHGHANRRSDDVSSTAYWYQLE
                     PHAPFGLAPVAERIPRPNGG"
     gene            4500..4574
                     /locus_tag="VIF63_02010"
     tRNA            4500..4574
                     /locus_tag="VIF63_02010"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Thr,seq:cgt)
     gene            complement(4624..5082)
                     /locus_tag="VIF63_02015"
     CDS             complement(4624..5082)
                     /locus_tag="VIF63_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238185.1"
                     /translation="MKSRRSLIALLVLAVVLVGGLAAYINLSTPQGVAMPWNLTGMGG
                     MNSSVGEHHPFDQAQAQTMTSLEVHVEQWPANEADANAWLSQTIDYGFNTVTITLRLR
                     PPYVPKPGGLPILLGYDTGGWVTVALREPLRSRSLIDGATGKVIPYPRAP"
     gene            complement(5215..5604)
                     /locus_tag="VIF63_02020"
     CDS             complement(5215..5604)
                     /locus_tag="VIF63_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8238186.1"
                     /translation="MKIVVTSVLVDDQEKALRFYTDVLGFEKKDDIPLGGDARWLTVV
                     SPQDRDGTELLLEPDGHPAAKPFKAALAEDGIPYTSFGVDDVQAEFDRMQALGVQFTQ
                     EPLAMGPVTTAVFDDTCGNLIQIAQRH"
     gene            complement(5618..5890)
                     /locus_tag="VIF63_02025"
     CDS             complement(5618..5890)
                     /locus_tag="VIF63_02025"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8238187.1"
                     /translation="MGDVFKALADPTRRAILDELSDRNGQTLFEICVRLSSRYGLGST
                     RQAVSQHLDVLEEAGLIRTRRAGRYKFHDLDTSPLREITTRWPTAE"
BASE COUNT          984 a         2016 c         2079 g          934 t
ORIGIN      
        1 gtcgtcaccg actgcgacga aacgcggccg gtcgaagacc cgtacgagcc gcactggtcg
       61 attcgcggct gcgcgatcgc cgcagcgacg acgttgaaca tcaccaccgc gaacgtctcg
      121 gtggcctata ccgcaccgcc caacacgacg ctcacctgcg acccgacgct ccacgtcggc
      181 tgcatcgccg cgatcaccgt cacctaccag tacgccacga cgacgccctt cgtgagctgg
      241 atcatccagc cgttcacgat gagccagacc tcgtcgatgc ccatcgaaag ggtgttccca
      301 tgaccgaccg atcaggcgcc gtgcgaggtg ccggcgtcga gtcgcacccg aaccagcgag
      361 gccaggcgat cgtcgtcatg gtcggcgcga tcctgctcgc cctcgccctc gtcgccctca
      421 tcgtcgatgg cggcaacgtc ctcgcccagc agcgcgtcgc ccagaacggc gtcgacgcga
      481 catcagaggc tggcgccgtg ctcctcgccg agcgactggc gggcgcgacc gcgccgtcgg
      541 gcggctggga cgtcaacatt gcgggccgac tcgcgcagac tgcggcggcc aacaacgtca
      601 ccgtcgaggc cgcctactac accgacatct gcggcatccc gctcagggct gacgggaccg
      661 gcgccatcga cgacgacacc cgcgtcgagg acctctcggt ggcgctccag gtcggcaatg
      721 cctcgcacat cctgcccggc ggcagcgcca ccgcacccga ctgcccgaat cgattggtcg
      781 ggcccgtcgc cggcgtgctc gtgatcgggc gcaaggacgt cgcggcatac gtcgccggcg
      841 cgatcggctt cagccagttc cgaatcaata cccgtgcgac cgcggtcgcc ggctacctcc
      901 agggctactg cgactcgagc gagggccatc actgcgcggt cttgcccgtg gcgatccctg
      961 tgaacgcggt gacgtgcgat gggagtggcg atgtggcggg cgggaccgag cactgggcgt
     1021 ggaaccagat cgtcaccgtt ccgctgtgca agtcgaaccc gggcaacgtt ggctggctcg
     1081 actggacgcc gccgggcggt ggggcgggcg agctcgtgtg ctcgatcgtg aacaccaaca
     1141 atccgtcgat catcctgccg tcgtggatga acgtgccctc gaccggcaat gtcaacggcg
     1201 gaggctcctg cgcggacgcc gtgaccggca tcagctacag cggcgttgag ggagcactcc
     1261 gaaagtacaa cggccaggtg atcctcgtgc cgcagttcga tctcacgtgc aaggccaaga
     1321 acaacgatcc ggacccgatc agctccctgc ccgcggtcgt gacggcgccg aactacggct
     1381 gcccgaacgc ccccggcggc ggcaccggcc agaacatctg gtaccggatg ccgagcttcg
     1441 cgttcttcca gatgtgcgag ccgtcgatgg ccggctgcgc cggccgtcag ggcgcctaca
     1501 tccagggcaa caactcggcc gagtgcgaca ccgggaacgg cggcacctcg tgccttgtcg
     1561 gcaagttcgt cgacatcatg tcgacgggga ccgtcggcgc gggcgtcggc tcgggtactg
     1621 gcagcaaggc cgtcggagtc cagctcatca aataggcccc cgcggcctac gcgggcaggt
     1681 cggtcggaac ctcgtcgccc ttgtcggaga tgagcttcac gccgccggcg gcggtgactt
     1741 cgaacgtcca gacgtgcttg ttgatgcagc ccgcggggca gtcgccccag ccgatcgtga
     1801 tgtcgatcga gtagccgcca ccggggagcg gcgcggcgac ccaatacttc gaggcgccga
     1861 tgacatcggg cgtcaggggc atcgcgcccg tgaacatcgg attggtcgcg atgacgaggg
     1921 ccgcggcttc gtcggccgtg gatgcgggcg cgacgatcgg ggtgggcgtg ggtcgcgcgg
     1981 tcggcgcggg agtgggcgac gcagcggcgc cggaacaggc ggcaaatgcg gccgcgaagg
     2041 cgagggcgag gagcactcga ttcagcatgg gccgagggac ggtcgacgtc gccacgcggt
     2101 tccccaccgg ctccggtcca gagacgggta ccctcgcggc atgccgaccc gcgccgacgc
     2161 ctgggacctc ctctgcgagt ggacgaagac cgacgcgctg cgccgccacg gcctggcggt
     2221 cgaggccgcc gtcgcgtggt acggcgagca caagttcggc atcgccggcg acgagctcga
     2281 gatctggcgc tcggccggcc tgttgcacga cttcgactac gagcgctacc ccgagaccca
     2341 cccgatgcgc ggcgcggacg agctgcgggc gcatggctac cgcgaggacg tcgtcgaggc
     2401 gatcctcggc cacggcgacc acaccggcat cgcgcgcacc agcctgctcg cgaagaccgt
     2461 gtatgcctgc gacgagatga cgggcttcgt gatcgcggtg acgtacgtcc gcccgagccg
     2521 gagcctcgac gaggtcgagg tgcgctcggt cgtcaagaag atgaaggaca agggcttcgc
     2581 ccggcaggtg ccgcgcgacc agctcgagaa gggcgcgacc gagctcggtg tcccgttcga
     2641 ggagcacgtc gagaacgtca tcgccggcct caagacgatc cggggcgagc tcggcctcta
     2701 gggcgcgccc ggaattggca gcccaggaac gccgcccggg atcgggaacc cggggatccc
     2761 gccggggatg gccatcggca cgatggactt gatgtccgtc ggcagcaacg ggccgagcac
     2821 cgtcaggaca atcggcacgg tcgtcttctc gagcagctgg ccgatcgtcg actcgtcgac
     2881 cgccaggcgg atgtcgtgga gcacgccgag gtccgtgagc gccgacagcg cgttgttcgg
     2941 gtcggtgtac gtgtgcagga tcacgatcag cgccgagaac agcaggatcg actcggctgc
     3001 gccgaaggcg gcgcccagga cgcggtccgt cgccttcgac acgccgccga ccgcgacgcg
     3061 cgacaggatc gtcttcgaga acaggttgaa gacgatgagc aggccggaga acaccaccga
     3121 atagccgacc atgtcggcgt actggggcgg aatcttgggg aagaactcgt tcagcagggc
     3181 accggccggc tcgcgcaggt atgccccggc gacgaacgag acgcccagga acacgaggcc
     3241 cgccagccgg cggacgaagc cgctgcggaa cccgccgaac acgctggtcg ccagcgcgcc
     3301 gagcatcagg acatcggcca tcgatccctc cccgcctccc tggtcgccgc cacgatacgc
     3361 agttcagccg ccattcgggc gcgggattcg ctcggcgacc ggtgcgagcc cgaatggcgc
     3421 atgcggctcg agctggtacc agtaggccgt gctcgacacg tcatcgctgc gccggtttgc
     3481 atggccgtgc tcgatcgtca cccggatcga ctcgcggaag tggacggggt cctcgacgtg
     3541 gaagcggtac agcgaggcct tgcccgacca gttcgggccg ccgggcagcg tgatgccctg
     3601 gtacgggccg gcgaacgcgt ccgtcgggca ccaggcggcg ccgaagtagt cctcggtccc
     3661 cgtgccatgc aggctcggcg ggaacggctc gccgtcgacg aagatcatgt cgtcgccttc
     3721 gccgtaccag ttccactcgg ccgtttcgcg caggttgtgg acgttcaggt tgcagccgac
     3781 gtagtggcca cggccgcggg cgtcgaggat cacgtagttg ccctcgcccg acgcgttctc
     3841 gccctcctcg agcagctggt ggttcgtgaa ggctgctgca tcgacgccgt cggtggggtt
     3901 caagcgtcgc cactgcgcgt ggaaccgggc catgtccgat gggagctcgt cgtacagctc
     3961 gtagtcgacg tagaagtaga gcttgacctc ggcggtctcg cactcgctct gggcggtgat
     4021 ccgcgcaccc gcggcgaacg gcatcgggaa ccagcacgcg aagccgcggc cgtcctgcgg
     4081 gctcatctgg agcggcatcg acgcgaagtt cgtcgtcagc ccgtgcccga cgccgaagaa
     4141 gtccccgatc gggacgttca cgctcggctc gggctcgccg tcccaccagg cgcgcaggac
     4201 caccttgcgc aggaagtcgg gctcgtcgct gccgagggtc atccagatgt ggcggaccgc
     4261 gccggcgcct tccatctccg cgaggacggc ctcctggccg ggctgcagat ggatccgatc
     4321 gtcgttgccg ccggtccgat cccagctcga tgcgcgtcgc gacgtggcgt ttcggagcac
     4381 cgcgagaccc gcgaggggac tgccgaaccg gatgccgagc gaatccatcc gccgaagcgt
     4441 accggccggc cgtgctccga atggctcgga tcgccgggcc ttctatactc ccgccccggg
     4501 ccgacatagc tcagcggtag agcagctgtt tcgtaaacag caggtcctcg gttcaaatcc
     4561 gagtgtcggc tccactccat cgcacggcag tcaccgccca gacttcgctg cgtgccgcgc
     4621 cgcctaggga gcccgtggat acgggatgac tttccccgtg gcgccgtcga tgagacttcg
     4681 ggaccgcaag ggctcacgca gggcgaccgt gacccacccg cccgtgtcat agccaagaag
     4741 gatcggcagg ccacccggtt tgggcacgta gggcgggcga agacgcaggg tgatggtgac
     4801 cgtgttgaat ccgtagtcga tcgtctgtga cagccaggcg ttcgcgtcag cctcattcgc
     4861 gggccactgc tcaacatgga cctcgaggct cgtcatggtc tgcgcctgcg cttggtcgaa
     4921 gggatggtgc tcgcccacgc tgctgttcat gccgcccatc ccggtcaggt tccagggcat
     4981 ggccacgccc tgtggcgtgc tgagattgat gtaggcggcg agaccgccca cgaggacgac
     5041 ggcgaggacg agaagggcga tgagcgagcg gcgtgatttc atgaatcaat agatcacacg
     5101 agcgtcgccc tggcgcaagg gaccaaaggt cgcgcctcgt ttggagagtg ggcaggtcct
     5161 cggctcaaat ccgagtgtcg gctccattcc cgtcgatcgt gccatcggcc gtgctcaatg
     5221 gcgctgtgcg atctggatga ggttgccgca ggtgtcgtcg aagacggccg tggtgaccgg
     5281 gcccatcgcg agcggttcct gggtgaattg cacgccgagc gcctgcatgc gatcgaactc
     5341 ggcctggacg tcgtcgacgc cgaacgacgt gtacgggatg ccgtcctcgg cgagggcggc
     5401 cttgaacggc ttcgcggcgg gatgcccatc gggctcgagc aggagctcgg tcccgtcgcg
     5461 gtcctgtggc gagacgacgg tgagccatcg cgcgtcgccg ccgagcggga tgtcgtcctt
     5521 cttctcgaat ccgagcacgt cggtgtagaa gcgcagcgcc ttctcctggt cgtcgacgag
     5581 gacgctggtg accacgatct tcattcggaa ctccttgcta ttcggccgtc ggccaacggg
     5641 tggtgatctc gcggagtggc gatgtgtcga ggtcgtggaa cttgtaccgg ccggcacgtc
     5701 tcgtccggat cagtccggcc tcctcgagca cgtcgaggtg ctgtgacacg gcctgcctcg
     5761 tcgatccgag cccgtacctc gagctcagcc ggacgcagat ctcgaacagg gtctggccgt
     5821 tgcggtccga cagctcatcg aggatggccc gacgagtggg gtccgccagc gccttgaaca
     5881 cgtcgcccac gaccagatga taggcaagtg gctacttgcg tgtcaatcca gggtgaagca
     5941 tgcgctgacg atctcctgct ggcgtcgaag ccaggtgtcg cggccggtca ggatgtgcgt
     6001 gttcgtcagg agc
//
LOCUS       DATBSW010000075         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_146753, whole genome shotgun sequence.
ACCESSION   DATBSW010000075 DATBSW010000000
VERSION     DATBSW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146753"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..>2122
                     /locus_tag="VIF63_02030"
     CDS             153..>2122
                     /locus_tag="VIF63_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8238188.1"
                     /translation="MTDLIERPPSELVPDEPVKDEATSQDRPSSDVVRTKGGLEVLKP
                     GRDIERARRELQGTPFAPPEASAPNAQERRDVPRRLDHRSESRPDTPIKVGTDLPLED
                     THAERILTTQSPQRPQATSRIRIEVDGRLVEGLEGQTILEVCRDNGIEIPTLCYEPKL
                     PGFGACRMCVVDVEGEEHPPISCSRTCEDGMKVQTQTEEIRRLRRTNLELIFSDHNAY
                     CLPPCQNKCPSHIDIPGFLKQNAEGNFRESTRIFKRTIPFPSVLGRVCPAPCEEHCRR
                     DEVDEAIAIRDSHRYAGDMVIKSMLDEDLDPPIPFDHQPTTGRRAAVIGSGPAGMAAA
                     YYLLLSGHDVTVFERDPAPGGMLRYGIPQYRLPKVEVLEAEYESVTRLGGKIVCNQGL
                     GRDFTLDDLQYQGFDAVVIAIGCYDTNKLGIPGEDAPEVLDGLEYLRTATLGLPYPEH
                     AGKRVVVIGGGFTSMDCSRTSIRQGAKEVTLVYRRDMKDMPASNEVHEAIEEGVTAIF
                     QAGPVRVITDKENNVTGVEFIRMAPGAPDASGRRRPEPAPGTEFTIPCDRVLLAIGQG
                     PDLTWIGPGSDGVEATKQNRLKADAVTFETGRPGVFGSGDVRVGAATVVQAIAEGRRA
                     AYAVDAFLRGNDLSTIRTRQTLAEPEPIFLSIV"
BASE COUNT          353 a          797 c          693 g          279 t
ORIGIN      
        1 gttgtcggat cgggcctgtg cgaccacgag cgactcgcaa cactgccgat gatgagcagc
       61 ctccgctatt tccgcgacga gatcgacgcg cacctcctgc gcggcgagtg cccggccggc
      121 gtctgccgcc cgatccgcct cggagccacg gcatgaccga cctcatcgag cgcccgccca
      181 gcgagctcgt gccggacgag cctgtgaagg acgaggcgac gtcgcaggac cgcccgtcga
      241 gcgacgtcgt ccggaccaag ggcggcctcg aggtcctgaa gccgggccgc gacatcgagc
      301 gcgcccgccg cgagctccag ggcacgccgt tcgccccacc cgaggcctcc gccccgaacg
      361 cccaggaacg cagggacgtc ccgcgccgcc tcgaccatcg ctccgagagc cgccccgaca
      421 ccccgatcaa ggtcggcacg gacctcccgc tcgaggacac gcacgccgag cgcatcctca
      481 cgacccagtc gccgcagcgg ccgcaggcca cgtcgcggat ccggatcgag gtcgacggac
      541 gcctcgtcga aggcctcgag ggccagacga tcctcgaggt ctgccgcgac aacggcatcg
      601 agatcccgac cctgtgctac gagcccaagc tgccgggctt cggcgcctgc cgaatgtgcg
      661 tcgtcgatgt cgagggcgag gagcacccgc cgatctcgtg ctcgcggacc tgcgaagacg
      721 gcatgaaggt ccagacccag accgaggaga tccgccggct gcggcggacg aacctcgagc
      781 tgatcttcag cgaccacaac gcctactgcc tgccgccgtg ccagaacaaa tgcccgagcc
      841 acatcgacat cccgggcttc ctcaagcaga acgccgaggg caacttccgg gagtcgaccc
      901 gcatcttcaa gcggaccatt ccgttcccga gcgtgctcgg ccgcgtctgc ccggcaccgt
      961 gcgaggagca ttgccggcgc gatgaggtcg acgaggcgat cgcgatccgc gattcgcatc
     1021 ggtacgccgg cgacatggtc atcaagtcga tgctcgacga ggacctggat ccgccgatcc
     1081 cgttcgacca ccagcccacc accggtcgcc gcgccgccgt gatcggctcg ggtccggcgg
     1141 gcatggccgc ggcctactac ctgctcctgt ccggccacga cgtcaccgtc ttcgagcgcg
     1201 acccggcgcc gggcgggatg ctccgctacg gcatcccgca gtaccgcctg cccaaggtcg
     1261 aggtgctcga ggccgagtac gagagcgtga cccgcctcgg cggcaagatc gtctgcaacc
     1321 agggcctggg ccgcgacttc accctcgacg acctgcagta ccagggcttc gacgccgtcg
     1381 tcatcgcgat cggctgctac gacacgaaca agctcggcat cccgggcgag gacgcgcccg
     1441 aggtcctcga cggcctcgag tacctgcgca ccgcgaccct cggcctgccc tatcccgagc
     1501 acgcaggcaa gcgcgtcgtc gtcatcggcg gcggcttcac ctcgatggac tgttcgcgga
     1561 cctcgatccg ccagggcgcg aaggaagtga cgctcgtcta tcgccgcgac atgaaggaca
     1621 tgccggcgtc gaacgaggtc cacgaggcga tcgaggaagg cgtcaccgcg atcttccagg
     1681 ccggcccggt tcgggtcatc accgacaagg aaaacaacgt caccggcgtc gagttcatcc
     1741 ggatggcgcc gggcgcgccg gatgcctcgg gcaggcgacg cccggaaccg gcgcccggca
     1801 ccgaattcac gatcccctgc gaccgcgtgc tgctggccat cggccagggt cccgacctga
     1861 cctggatcgg gcctgggtcc gacggcgtcg aggcgaccaa gcagaaccgg ctcaaggccg
     1921 acgcggtgac cttcgagacc ggccggcccg gcgtgttcgg cagcggtgac gtccgggtcg
     1981 gcgccgcgac ggtcgtccag gcgatcgccg agggccgccg cgccgcctac gcggtcgacg
     2041 cgttcctgcg gggcaacgac ctgtcgacca tccgcacccg tcagacgctc gccgagcccg
     2101 agccgatctt cctgtcgatc gt
//
LOCUS       DATBSW010000076         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_182399, whole genome shotgun sequence.
ACCESSION   DATBSW010000076 DATBSW010000000
VERSION     DATBSW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182399"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VIF63_02035"
     CDS             <1..487
                     /locus_tag="VIF63_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238189.1"
                     /translation="RDVLGTGSKAIFGIRLSLLVIVVVIGITSWSSTARIIRSQTLSL
                     KERAFVDRARVIGASGGHIMRRHILPNVVNLIVANAVLTFAGAILTETTLSFVGLGDP
                     FQPSWGQLLDASLTVGAPGLGKWWYFVPAGTAVVLVVFAFTLLGAALDDILNPKLATR
                     R"
     gene            484..1614
                     /locus_tag="VIF63_02040"
     CDS             484..1614
                     /locus_tag="VIF63_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238190.1"
                     /translation="MTLRSGIVSRRQWPLPKLADPNAPLLEVKDLQTHFKLERGWLRA
                     VDGVSFRLDDGEALGLAGESGCGKTTTALSLVRLLPSNGQIVGGSVELFGIDLVPKSE
                     RALQRYRWREISIVFQGAMNAFNPVRKVRDQIAEPLRYRLDQSEKQSRARAVELLDLV
                     GIPRKRADAYPHELSGGMRQRAMIAMALACDPAIVIGDEPTTALDVMVQAQILQLLEQ
                     LRTDLGLSLILITHDLSVIAETCDKVLIMYAGRVAEEGPVSRVFTAPRHPYTKKLLGA
                     FPSIHADRRTLDVIPGQPPDLVSPPGGCRFAPRCGFVMPICTEVVPPEIRFTDGVRVA
                     CHLYPGSDAASVANVEEAPAMAPIPPSSGPADPPSEISAPPA"
     gene            1611..>2003
                     /locus_tag="VIF63_02045"
     CDS             1611..>2003
                     /locus_tag="VIF63_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8238191.1"
                     /translation="MTELLRLSGLQVHFPIRHGVGDSLLGRKPQVVRAVDGIDLTIEQ
                     GEVLALVGESGSGKTTTGRVIVKLTRQTGGSMHFDGQDVSKLWGTGKLRSFRRRVQLI
                     FQDPYETLNPKQSIHDFVAEPLLVNKIGS"
BASE COUNT          287 a          724 c          672 g          320 t
ORIGIN      
        1 ccgggacgtg ctcggtaccg gctccaaggc gatcttcggg attcggctga gcctgctcgt
       61 gatcgtcgtc gtcatcggca tcacgagctg gtcgtccacc gcccgaatca tccgttcgca
      121 gacgctgtcg ctgaaggagc gcgcgttcgt cgatcgagcg cgcgtgatcg gggcctcggg
      181 tgggcacatc atgcggcggc acatcctgcc gaacgtcgtg aacctgatcg tggcgaacgc
      241 tgtgttgacg tttgccggcg cgatcctgac cgagacgacg ctgtcgttcg tcggcctcgg
      301 cgacccgttc cagccgtcgt ggggccagct gctcgacgca tcgctcaccg taggtgcgcc
      361 cggcctcggg aagtggtggt acttcgtccc cgcgggcacg gcggtcgtgc tcgtcgtctt
      421 cgcgttcacg ctgctcgggg ccgccctcga cgacatcctc aacccgaagc tggcgacgcg
      481 ccgatgacgc ttcgaagcgg catcgtgagc cggcgtcagt ggccgctgcc gaagctggcc
      541 gaccccaacg cgcccctgct cgaagtcaag gacctccaga cccacttcaa gctcgagcgc
      601 ggttggctgc gggcggtcga tggcgtgtcg ttccggctcg atgacggcga ggcactcggc
      661 ctcgcgggtg agtcgggctg cggcaagacg acgaccgcgc tctcgctcgt gcggctcctg
      721 ccgtcgaacg gccagatcgt cggtggcagc gtggagctgt tcggcatcga cctcgtgccg
      781 aagtccgagc gcgcgttgca gcgctaccgc tggcgcgaga tcagcatcgt gttccagggc
      841 gcgatgaacg cgttcaaccc ggtccggaag gtgcgcgacc agatcgccga gccgctccgc
      901 taccgcctcg accagtccga gaagcagtcg cgcgcccgcg ccgtcgagct cctggacctc
      961 gtcggcatcc cgcgcaagcg cgcagacgcg tacccgcacg agctgtcggg cggcatgcgc
     1021 cagcgggcga tgatcgcgat ggccctcgcc tgcgacccgg cgatcgtgat cggcgacgag
     1081 ccgacgaccg ccctcgacgt catggtccag gcccagatcc tccagctcct cgagcagctc
     1141 cggaccgacc tcgggctgtc gctgatcctg atcacccacg acctgtcggt catcgccgag
     1201 acctgcgaca aggtcctgat catgtacgcc ggccgggtcg ccgaggaggg cccggtgagc
     1261 cgcgtcttca ccgcgccgcg gcacccgtat acgaagaagc tgctcggggc gttcccgagc
     1321 atccacgccg accgtcggac gcttgacgtc atccccggcc agccgccgga cctcgtgtcg
     1381 ccgccgggcg gctgccggtt cgcgccgcgc tgcgggttcg tcatgccgat ctgcacggag
     1441 gtcgtgcccc cggagatccg cttcaccgac ggcgtgcgcg tcgcctgcca cctgtacccg
     1501 ggttcggacg cggcgtcggt cgcgaacgtc gaggaagccc cggcgatggc gccgatcccg
     1561 ccgagctccg gcccggccga cccaccgagc gaaatctcgg ccccgcccgc atgaccgagc
     1621 tgctgcggct ctcgggcctc caggtgcatt tcccgattcg gcacggggtc ggcgactcgc
     1681 tgctcgggcg gaagccgcag gtcgtgcgcg ccgtcgacgg catcgacctc acgatcgagc
     1741 agggcgaggt cctcgctctc gtcggcgaat ccgggtcggg caagacgacg accggccggg
     1801 tgatcgtcaa gctcacccgc cagaccggcg gctcgatgca cttcgacggc caggacgtct
     1861 cgaagctgtg gggcacgggc aagctgcgca gcttccgccg ccgcgtgcag ctcatcttcc
     1921 aggacccgta cgagacgctg aatcccaagc agtccatcca cgatttcgtg gccgagccgc
     1981 tcctcgtcaa caagatcggc tcg
//
LOCUS       DATBSW010000077         6254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_393733, whole genome shotgun sequence.
ACCESSION   DATBSW010000077 DATBSW010000000
VERSION     DATBSW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393733"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VIF63_02050"
     CDS             complement(<1..289)
                     /locus_tag="VIF63_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238192.1"
                     /translation="MSRRRLGAIGLIAIVGFVVACDPSPSQPPLATSQALASASPEQS
                     APSSSAAATFDPAVPSVPIQTIAFDGLYASKDVSVSGDTTVAVVRSGMTCPA"
     gene            complement(286..2319)
                     /locus_tag="VIF63_02055"
     CDS             complement(286..2319)
                     /locus_tag="VIF63_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238193.1"
                     /translation="MTRMPGRRRIAVAVCLAVILPFGGTVGLAVGQRGGTGATDATGR
                     VYSFADLPADPAKAGIALVDTILGSDAEAANAATIELFRRSGMPVVSAKGPVVALPDD
                     YVLVDAAIYDEFVPWLTTSVRRGDSLSPDQFAGYMNDLGAFPGELPPSVLLGGLGTWG
                     KDKDSGPEERTAAAAIRALSAQRGEVLFMEADAENTHLDMLQLELVLGTLSFVGRIEP
                     ANPKGLDSAFAPGGAPPTALEQLLGVRSAEAASPCEQLEKALKGPSELGYNIAWNQSK
                     KGAKKAAGSLFTQWRKSNPTGAVQRMLDKADALKEGSKASDVWSHGSEILNILLIYLG
                     ATLDVNAKPPGLHTMPEGGVPQVEVEAKANFESTLAQGQLPCYAMVGIEVPPNKPLSG
                     YKVHWSITQPVVGAGSGQLLQAVEGEYFGGGGNQAQITGGAGEAKVHFKVATERSPGA
                     GQLKRKTVVVRATLDKDDFPFKLGDLLGAGKPGWALQKSWDLVVSAAKRKALPSTPEN
                     INIAYHDAESYSIKGTRTILAFYYVMPITLDLYSCDGIEGPWHGTVKFHGDIAFMGDP
                     ANQVFGNKFPPTVDQSWTVQFPMHMGPPPTIAGDIQEGRAELVGGLGGIVEMDGGWRL
                     LDRRDGSVVGRIILTGDGGETLDTLAIWDGFDASYPIKVGAPSHCPPSGLYFP"
     gene            complement(2392..3450)
                     /locus_tag="VIF63_02060"
     CDS             complement(2392..3450)
                     /locus_tag="VIF63_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY8238194.1"
                     /translation="MLADRFKVWKTAYNTANLPVGMTTPPDSISKWLVITRAAVFSMT
                     VTSGLIGGLLAVGAAQLAQVGAGPIGGPYEVNYGLLLLAIVGLVVAHAANNMINDYFD
                     LEGGIDTDDYVRAQYAPHPILSGWVTKRQLGTAILLMNAIDLAILVFLMTQRGPAVAF
                     FALGGLFVSVFYVAPPIRLKHIGLGEPGVFVVWGPLMVVGTFFVATGQIPGWVWIASL
                     PYAILVTSVLFGKHIDKIDADQMKGVRTMPVLLGERRARLVAQGLMIAFYPIVLGAVV
                     VGWIGPWVALVVLALPRLVQVLKVFGQPRPDVEPHSYVGWPLWFVGQAFIHTRRAGAL
                     LILGLLLNAFLPVKLPWL"
     gene            3584..4270
                     /locus_tag="VIF63_02065"
     CDS             3584..4270
                     /locus_tag="VIF63_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238195.1"
                     /translation="MATSIRVPAIGLLAAFMVAACAGAAVPPGTAQPSSEGPAGPSSG
                     ASSLAEGTWTGTLTRHYTETTTTTSESDSTLVVVTYDATVLISSTLVDIGGWKLTGPA
                     TIRTTQTSDYKAHFTSTLGDCDKHYHDDDLAEGTSIVDGGLGISDDATYEFTVTVPGF
                     DGGTETSLRDETGCQGSSNTEVNPWPVGPVNLEGSGEITDPAFLTGSTSKPIANGGQD
                     TTTWSLRLSK"
     gene            4307..5515
                     /locus_tag="VIF63_02070"
     CDS             4307..5515
                     /locus_tag="VIF63_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY8238196.1"
                     /translation="MRPEELDPSLTIETLAVHAGQEPDAQTGAVAPPIYQTSTYRQPK
                     VGQPIDDYDYARSKNPTRDRFERAVAALEGGGYGIAFASGSATTAAIAELAGPDTEVV
                     VGDDVYGGTFRYLDKVRGPNRVTTRYADLAADPEAMWEALSEKTRLVWFETPTNPLLK
                     VVDIRASADVVRERFGDGPRPLVVVDNTFASPILQRPLDLGADIVFHSATKYLAGHSD
                     TVLGVAVTRDKALADRLHFLQNAMGGVPGPFDCFLALRGLRTLPLRVGRHAANASEVA
                     RFLAERDDIEWLSYPGLASGPHAHPQAACASRQMRAGGGMVSFVPAARGGRTARERAI
                     AICESTRIFTLAESLGGVESLIELPAVMTHLSVAGSHLEVPEALVRLSVGIEGAADLI
                     ADLAQALDRS"
     gene            5585..>6254
                     /locus_tag="VIF63_02075"
     CDS             5585..>6254
                     /locus_tag="VIF63_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238197.1"
                     /translation="MRTHAQLRRLAAGLAAGLAALGILASIQLTPLVAVAADTPGGLA
                     VAAVDPAGTSLVGTCFELYTDAGGGTLGKLVVDGKRCDKARRADGKAGPEDGLLDGVT
                     AWSNLAPGAFVLHEVLGPALAGTAATYEVGPDTAVSISAGAVTSLRLVHRPQPGLTLH
                     KVDATGRALPGACFSLSALDRAAVAACDGPRPQGSVPAGDGLADGLLVFTALAPTGKP
                     TRYTL"
BASE COUNT          979 a         2215 c         2105 g          955 t
ORIGIN      
        1 gggcgggaca ggtcatcccg ctgcgcacga cggcgaccgt cgtgtcgccg ctcacgctca
       61 catccttgct ggcatagagc ccgtcgaagg cgatcgtctg tattggcacc gacggcacgg
      121 caggatcgaa cgtcgcagcc gcggaagacg atggcgcgct ctgttccggg ctcgccgacg
      181 caagcgcctg gctcgtggcc agcggtggct gggagggcga cggatcgcac gctacgacga
      241 acccgacgat cgcgatcagc ccgatcgccc cgaggcgtcg tcggctcatg ggaagtacag
      301 cccgctcggc ggacagtggc tcggtgcacc aaccttgatg gggtaggacg catcgaagcc
      361 atcccagatc gccagcgtgt cgagcgtttc gccgccgtct ccagtgagga tgattcgacc
      421 gacgacggac ccatcgcggc gatcgaggag tcgccagccg ccgtccatct cgacgattcc
      481 gcccaggcca ccgacgagct ctgcgcgtcc ctcctggatg tcccctgcga tggtcggcgg
      541 cggacccata tgcatcggga attgcaccgt ccaactctgg tcgaccgtcg gcgggaactt
      601 gttgccgaac acctggttcg ctgggtcgcc catgaaggca atgtcaccgt ggaacttgac
      661 ggttccgtgc cacggaccct cgatgccgtc gcagctgtac aggtcgaggg tgatgggcat
      721 cacgtaatag aaagcgagga tcgttcgcgt gcccttgatc gagtacgact cggcgtcgtg
      781 gtaggcgatg ttgatgttct ccggggtcga tggcagcgcc ttgcgcttgg ccgcggacac
      841 caccaggtcc cacgacttct gcagcgccca gcccggcttg ccggcaccca ggaggtcgcc
      901 gagcttgaac gggaagtcgt ccttgtcgag cgtggcccga acgacgacgg tcttgcgctt
      961 gagctgaccc gcgccggggc tgcgctcggt agcgaccttg aagtggacct tggcctcgcc
     1021 ggcgcccccc gtgatctgcg cctgattgcc gccgcccccg aaatactcgc cctcgaccgc
     1081 ctgcaggagc tggccactgc ctgccccgac caccggctgc gtgatggacc agtggacctt
     1141 gtagccggag agtggcttgt tcgggggaac ctcgatgccc accattgcgt agcacggcaa
     1201 ctgtccctgc gcgagcgtcg actcgaaatt cgccttggct tcgacctcga cctggggcac
     1261 gccgccttcg ggcatggtgt gcaggcccgg cggctttgcg tttacgtcga gcgtcgcgcc
     1321 cagatagatg agcaggatgt tcaggatctc ggacccgtgg gaccagacgt ccgaggcctt
     1381 cgagccttcc ttcagcgcgt cggccttgtc cagcatccgc tgcacggcac cggttggatt
     1441 gctcttccgc cactgggtga agagactgcc ggcggccttc ttggcgccct tcttcgattg
     1501 gttccaggcg atgttgtaac caagctcgct cgggcccttc agggccttct ccagctgctc
     1561 gcaggggctg gcggcctcgg cggagcggac gcccaggagt tgctcgagcg ccgtcggcgg
     1621 cgcgcccccc ggcgcgaatg ccgagtcgag cccctttgga tttgccggct cgatcctgcc
     1681 cacgaagctg agcgtcccga gcacgagctc gagctggagc atgtcgagat gcgtgttctc
     1741 ggcgtcggcc tccatgaaca ggacctcgcc gcgttgcgcg ctcagcgcgc gaatcgccgc
     1801 ggccgccgtg cgttcctcag gcccgctgtc cttgtccttg ccccaggttc ccaggccgcc
     1861 caggagcacg ctcggtggca gctcaccggg gaaggccccc aggtcgttca tgtagcccgc
     1921 gaactggtcg ggcgacagcg aatcgccgcg ccggacggag gtggtcagcc acgggacgaa
     1981 ctcgtcgtag atcgcggcat cgaccagcac gtagtcgtcc ggcagcgcca cgaccggacc
     2041 cttcgccgag acgaccggca tccccgagcg ccggaacagc tcgatcgttg ccgcatttgc
     2101 ggcttccgcg tcgctaccga ggatcgtatc gaccagcgcg atcccggctt tcgccggatc
     2161 cgccggcaga tcggcaaacg agtagacgcg accggtcgcg tcggtcgcac cggttccgcc
     2221 gcgctgacct acggccaggc cgacggtccc tccgaacggc aggatcaccg ccaggcagac
     2281 agccaccgcg attcggcggc ggccaggcat gcgcgtcatg ccgtcaatcc tgagaggtca
     2341 cgcgctccgt ttcatcgttc gattgcgcca actgtggcga ccagccggac cctagagcca
     2401 gggcagcttg accggcagga acgcgttcag caggaggccg aggatcagga gcgcgccggc
     2461 gcggcgggtg tggatgaacg cctggccgac gaaccacagc ggccagccga cgtagctgtg
     2521 gggctcgaca tccggcctcg gctggccgaa caccttcagc acctggacga gccgcggcag
     2581 ggcgagcacc acgagtgcga cccagggccc gatccagccg acgacgaccg caccgagcac
     2641 gatcgggtag aacgcgatca tcaggccctg cgcgaccagg cgagcgcggc gctcgccgag
     2701 cagcacgggc atggtgcgga cgcccttcat ctggtccgcg tcgatcttgt cgatgtgctt
     2761 gccgaagagg acgctggtca cgaggatcgc atagggcaac gaggcgatcc agacccagcc
     2821 cgggatctgg ccggtcgcga cgaagaacgt gccgacgacc atcagcgggc cccacacgac
     2881 gaacacgccc ggctcgccga gcccgatgtg cttcagccgg atgggcggcg cgacgtagaa
     2941 cacggacacg aacaggccgc cgagcgcgaa gaaggcgacg gccggcccgc gctgggtcat
     3001 gaggaacacg aggatcgcga ggtcgatcgc gttcatgagg aggatcgccg tgccgagctg
     3061 gcgcttcgtg acccagcccg acaggatcgg gtggggcgcg tactgcgccc ggacgtagtc
     3121 gtccgtgtcg atgccgccct cgaggtcgaa gtagtcgttg atcatgttgt tcgccgcgtg
     3181 agcgacgacc agcccgacga tggcgagcag cagcaggccg tagttgacct cgtacgggcc
     3241 gccgatcggc ccggccccga cctgggcaag ctgcgccgcg ccgacggcca gcagtccgcc
     3301 gatcagccct gacgtcacgg tcatcgagaa gaccgccgct cgggtgatga cgagccactt
     3361 cgagatcgag tccggcgggg tcgtcatccc caccggcagg ttggccgtgt tgtaggcggt
     3421 cttccagacc ttgaaacggt cggcgagcac ggtggtgggc cctcgaagcg ctcggaagat
     3481 cggtcttccg aaacttgcgc gtcgatagta gcactattcc ggaagcgtat ggttccgcgt
     3541 gtcgagcaac ccatcgctcg cgtccgccac gtgaggatcc gccttggcaa cctcgatcag
     3601 ggtgcccgcg atcgggttgc ttgccgcctt catggtcgcc gcctgtgcgg gcgccgccgt
     3661 tcctccgggc accgcacagc cctcgtcgga ggggccggcc ggtccatcgt cgggggcgtc
     3721 gagcctggcc gaggggacgt ggaccggtac gctgacgcgc cactacaccg agaccaccac
     3781 gacaacgagc gagagcgact cgaccctggt cgtcgtcaca tacgacgcga cggtgctgat
     3841 ctcttcgacg ctggtcgata tcggcgggtg gaagctgacc ggaccggcga cgatcaggac
     3901 gacgcaaacg agcgactaca aggcccactt cacctcaaca ttgggcgact gcgacaagca
     3961 ctaccacgat gatgacctcg ccgagggcac gagcatcgtc gatggcgggc tcggcatctc
     4021 cgacgacgcg acgtacgagt tcaccgtgac cgtcccgggg ttcgacggcg gcacggagac
     4081 ctcgttgcgt gacgagaccg gctgtcaagg ctcgagcaac accgaggtga atccctggcc
     4141 ggtcggcccg gtcaacctcg aagggagcgg agagatcacg gatccagcct tcctgacggg
     4201 cagcacgtcc aaaccgatcg ccaacggtgg gcaggacacc accacctgga gcttgcggct
     4261 cagcaagtga ccatgaaccg ttcccgtgat gcgatcatct gcacagatgc gacctgagga
     4321 gctcgacccg agcctgacga tcgagaccct ggcggtccac gccggccagg agccggacgc
     4381 ccagaccggc gccgtcgcgc cgccgatcta ccagacctcg acgtaccggc agccgaaggt
     4441 cgggcagccg atcgacgact acgactacgc gcgctcgaag aacccgacgc gggaccggtt
     4501 cgagcgtgcc gttgcggccc tcgagggcgg cggctatggc atcgcgttcg cttccggctc
     4561 ggccaccacg gccgcgatcg ccgagctggc cggccccgac acggaggtcg tcgtcggtga
     4621 cgacgtgtac ggcggcacgt tccgctacct cgacaaggtc cgcgggccga accgggtcac
     4681 cacccgctac gccgacctcg ccgccgatcc cgaagccatg tgggaggcgc tctcggagaa
     4741 gacgcggctc gtctggttcg agacgccgac caacccgctg ctcaaggtcg tcgacatccg
     4801 ggcctcggcc gatgtcgttc gcgagcggtt cggcgacggg ccccgaccgt tggtcgtcgt
     4861 cgacaacacc ttcgcctcgc cgatcctcca gcggccgctc gacctcggcg cggacatcgt
     4921 cttccactcc gccaccaagt acctcgcggg tcacagcgac acggtgctgg gcgtggcggt
     4981 gacgcgcgac aaggcgctgg ccgaccgcct gcacttcctg cagaacgcga tgggcggcgt
     5041 gcccggcccg ttcgactgct tcctggcgct gcggggtctg cggaccttgc cgctgcgcgt
     5101 cggccggcac gcggcgaacg cgtcggaggt tgcccgcttc ctcgcggagc gcgacgacat
     5161 cgagtggctg tcatatcccg ggctcgcgtc cggcccgcat gcgcacccgc aggcggcgtg
     5221 cgcgtcgcgc cagatgcgcg cgggtggggg catggtctcg ttcgtgcccg cggcgcgcgg
     5281 cggcagaacc gcgcgggagc gcgccatcgc gatctgcgag tcgacgcgca tcttcaccct
     5341 cgccgagtcg ctcggtggcg tggaatcgct catcgagctc ccggccgtga tgacccacct
     5401 gtccgtcgcc ggctcgcacc tcgaagtgcc cgaggcgctc gtgcgcctgt ccgtcggcat
     5461 cgaaggcgcc gccgacctca tcgcggacct ggcgcaggcg ctcgaccgct cctaggccac
     5521 gtggccgcgc gatttggcgc aaccgaacga tgacggacgc gccgcgtgca gcgaccgtgc
     5581 gggcatgcga acgcatgcgc agctccgacg cctcgccgcc ggcctcgccg ccggcctcgc
     5641 cgctctcggc atccttgcct cgatccagct gacgccgctc gtcgccgtcg ccgccgacac
     5701 gccgggtggc ctcgccgttg ctgccgtcga tcccgccggc acgagcctgg tgggcacctg
     5761 cttcgagctg tacaccgatg ccggcggcgg aaccctgggc aagctggtcg tggacggcaa
     5821 gcgctgcgac aaggcccgcc gcgccgacgg caaggccggc cccgaggacg gcctcctcga
     5881 cggcgtcacc gcgtggtcga atctcgcgcc gggtgccttc gtcctgcacg aggtcctggg
     5941 cccggccctc gccggcacgg ccgcgaccta cgaggtcggg ccggacacgg cggtctcgat
     6001 cagcgccggg gcggtcacca gcctgcgcct cgtccaccgg ccccagccgg gcctgaccct
     6061 gcacaaggtc gatgccacgg gccgggccct gcccggtgcc tgcttcagcc tgtccgcgct
     6121 cgaccgagcc gctgtcgccg cctgcgacgg accccgcccc cagggctcgg tcccggccgg
     6181 tgatggcctt gccgatggcc tgctcgtgtt caccgccctg gcgcccaccg gcaagcccac
     6241 caggtacacc ctca
//
LOCUS       DATBSW010000078         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_675848, whole genome shotgun sequence.
ACCESSION   DATBSW010000078 DATBSW010000000
VERSION     DATBSW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675848"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..>3134)
                     /locus_tag="VIF63_02080"
     CDS             complement(121..>3134)
                     /locus_tag="VIF63_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238198.1"
                     /translation="MVIESGGRLFPKLLDFGISKILGAAGGSDEEAHEDGGPDRAAIT
                     TARLRLAPPDRTPTWSGKGKGTIRLTPSGALLGSRPYMSPEQWRDSHGVGPATDIYAL
                     GCLAHEALTGRTPFVADNASDYHDCHLHAEAPSLGEGFPPGADQAIRRALSKSPEARQ
                     ETALKLAAEFRAALRAEPREQLRSAAQRWEDRARSTAVLWTSKDLSEVARAVPSKAMS
                     ELECSFVAASQRRARRRAWAQRVLVAVVAGSALLAFVGRAAMQARAARELAEAKISQS
                     ELEQGRAALLHGEPEAQRHLAEAYKREPGAATAFMLARAMQPRLSELARLQGTYGRMW
                     WATFSPDGSQVATADDRAAQIWDGQTHRLLFTLPHGTEVYQAIYAPDGAWLVTVTGTT
                     IKIWDAKTGMLIRDLAAPPDEPTRPNFQRAAISSTGKLVAAMYVDGSVTRVWEAESGK
                     LVAELRDPHPDAIVRLAFSRNDYLATTGGNDAHVFDVRTWRRVLTVSGPVRSLAFDAH
                     GRLATGSATGDVSLFEIPSGQRLRQLRQSGESVESLAFSSDGELVAAGGRDGTMQVWW
                     TGSGALRSQLNPRRSGILAVEFDPTSASLLAAHADGTAVVADTAQGLPIAILDGPRSA
                     LRVARFGPRGQVVGAFRDGTARIWDATAPYRRWGSAPMSDDCGVPGVQPDGRFVAVGC
                     GARPTRVWDTAHARLLAELPGVTPIESGDFTSAFPAVSAEGDRAAIARGNAVELYALP
                     GGRLLRRIEHAAPVSAVAFAPMGRDVVSGAVDGSILVTRDDGASRALQAAAGIDVAEL
                     LPDGSVVAGDAGRRLRVFSPSGAVLADLEIPVRVMSLRREGARLVALPSYLAAASPPL
                     VVDIAPPRIIARLEGHTGQVVSARWIAQGRIMTAGADGTARIWNGTTGTLLQTYQGWP
                     GLLADATLTPDGLVIGGDADGSLRFWDAASGAKLWTLPAHKSAVIGVHLEGADIVTRG
                     FAGEISRWTLPQPGAVMDACAHHPPCAMIAR"
BASE COUNT          461 a         1126 c         1099 g          448 t
ORIGIN      
        1 ccgattgcgg gtaggcatcg acggtatccg tttcctgtcc gccgatgacg tgcgtaagtg
       61 ctttttcgga gagctcccgg acggtctcct tgcgcaacac gagcttgcga gtcgttttct
      121 tcatcgcgca atcatagcgc aggggggatg atgggcgcag gcgtccatga ccgccccggg
      181 ttgcggcagg gtccagcgcg agatttcccc ggcgaatccg cgcgtcacga tgtcggctcc
      241 ttcgaggtgc acgccgatca cggcggactt gtgcgccggc aacgtccaga gcttggcgcc
      301 gctcgcggcg tcccagaatc gcagcgagcc gtcggcgtcg cccccgatca ccaggccgtc
      361 cggcgtgagg gtcgcgtcgg caagcaagcc gggccatccc tgatacgtct ggagcagcgt
      421 tcccgtcgtc ccgttccaga tgcgggccgt cccatcagcg cccgccgtca tgatgcgccc
      481 ctgagcaatc cagcgcgccg agaccacctg gccggtgtgg ccctcgagcc gcgcgatgat
      541 gcgaggcggg gcgatatcga cgacgagcgg tgggctcgcg gccgccaggt agctcggaag
      601 ggcgacgagg cgggcgcctt cgcggcggag cgacatcaca cgtacgggta tctcgaggtc
      661 ggcgaggacc gcgccgctcg gactgaacac gcggaggcgt cgtccagcgt ctcccgcgac
      721 gacgctgcca tccggcagca actccgcgac atcgatcccg gccgcggcct ggagcgcgcg
      781 gctggctccg tcgtcacgcg tgacgaggat cgagccgtcg acagccccgc tcaccacgtc
      841 ccggcccatg ggcgcgaagg ccacagcgct cacgggtgcg gcgtgctcga ttcggcgcaa
      901 caggcgaccg cctgggagcg cgtacagttc gacggcgttg ccacgagcga tggcggcacg
      961 gtcgccctcg gccgagaccg ccgggaacgc cgacgtgaag tcgccgctct cgatcggggt
     1021 gacgcccggc agctcagcga gcaagcgagc gtgggcggtg tcccacacgc gcgtgggccg
     1081 cgccccgcaa ccgacggcga cgaaccgacc gtctggctgc acgccgggga cgccgcagtc
     1141 atcgctcatc ggcgccgaac cccaccgccg atacggggcg gtcgcgtccc agatccgggc
     1201 ggtgccgtcg cggaacgcgc cgacgacctg cccacgcgga ccgaacctgg cgacgcgcaa
     1261 ggcgctccgc ggcccatcga ggatcgcgat cggcaagcct tgcgcggtgt ccgcgaccac
     1321 ggcggtgccg tcggcgtgcg cggcgaggag cgacgccgag gtcgggtcga attcgaccgc
     1381 aaggatcccg ctccgacgcg gattgagctg gctccgcagc gcgcccgagc ccgtccacca
     1441 cacctgcatt gtgccgtcgc gaccaccggc ggccacgagc tcaccgtcgg acgagaacgc
     1501 cagcgactcg acgctttcac cggactggcg cagttggcgg aggcgctgtc cactcgggat
     1561 ctcaaagagc gacacgtcac cggtggccga gcccgtcgcc aggcgaccgt gggcgtcgaa
     1621 cgccaagctg cggacggggc cggagacggt gagcacgcgc ctccacgtcc ggacgtcgaa
     1681 gacgtgcgcg tcgttccccc cggtcgtcgc gagatagtcg ttccgactga aggccaggcg
     1741 cacgatggcg tcgggatgag gatcccgcag ctccgcgacg agcttgccgc tctcggcctc
     1801 ccagacccgc gtcaccgagc cgtcgacgta catcgcggca acgagcttcc cggtcgacga
     1861 gatcgccgcg cgctggaagt tcggccgcgt cggctcgtca ggcggagcgg cgaggtcgcg
     1921 gatcaacatt cccgtcttcg cgtcccagat cttgatcgtc gtccccgtga ccgtgacgag
     1981 ccaggcgccg tcgggggcgt agatggcctg atagacctcg gtgccgtggg gcagcgtgaa
     2041 cagcaggcga tgcgtctggc catcccagat ctgagcggcc cgatcgtccg ccgtcgcgac
     2101 ctggctgcca tcgggcgaga acgtggccca ccacatgcgg ccgtacgtcc cctggaggcg
     2161 cgcgagctcg ctcaggcgcg gctgcatcgc gcgggcgagc atgaacgccg tcgcggctcc
     2221 gggctcgcgc ttgtacgcct cggccaggtg gcgctgcgcc tcaggctcgc cgtgcagcag
     2281 cgccgcccga ccctgctcaa gttcggactg gctgatcttc gcctctgcca gctcccgcgc
     2341 ggcccgcgcc tgcatcgccg cgcggcccac aaaggcgagg agcgcgctgc cggcgacgac
     2401 cgcgacgagc acccgctgcg cccatgcgcg acgccgggcc cgccgctggc tcgccgccac
     2461 gaacgagcat tcgagctcgc tcatcgcctt cgaggggacc gcgcgcgcga cctcgctcag
     2521 gtccttggag gtccacagga cggccgtcga ccgagcgcgg tcctcccacc gctgcgccgc
     2581 cgaccggagt tgctcgcgcg gctctgcgcg cagcgccgcc cggaactcgg ccgctagctt
     2641 gagggcggtc tcctggcgcg cctccggaga cttggacaac gcacgacgga tggcctggtc
     2701 ggcgcccggc gggaaccctt caccgaggga cggcgcctcg gcatggaggt ggcaatcgtg
     2761 gtagtcgctg gcgttgtcag ccacgaatgg cgtgcgtccg gtgagagcct cgtgcgcaag
     2821 gcagccgagg gcatagatgt cggtcgccgg accgactcca tgcgaatcgc gccactgctc
     2881 cggggacatg tacggcctcg agcccagcag cgcgccgctg ggcgtgaggc ggatcgttcc
     2941 tttaccttta ccggaccaag tgggcgttcg gtccggcggt gcgagtcgga gcctcgcggt
     3001 cgtgattgcg gcgcggtcgg gccctccatc ttcgtgagcc tcctcgtcgg acccacccgc
     3061 ggcgccgagg atcttcgaga tcccgaagtc gagcagcttg ggaaacagcc ggcctccgct
     3121 ttcgatcacc atga
//
LOCUS       DATBSW010000079         7963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1169704, whole genome shotgun sequence.
ACCESSION   DATBSW010000079 DATBSW010000000
VERSION     DATBSW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169704"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VIF63_02085"
     CDS             complement(<1..181)
                     /locus_tag="VIF63_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238199.1"
                     /translation="MDALIANKSLRRKGVAYVVLVVASLILLTISANPLVRDLQHGIA
                     FAFRPVQVAVTGFAGD"
     gene            complement(195..1247)
                     /locus_tag="VIF63_02090"
     CDS             complement(195..1247)
                     /locus_tag="VIF63_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8238200.1"
                     /translation="MLDRILGLFSRDIGIDLGTANTLVHVRDRGIVISEPSVVAIEAK
                     SKRVLAIGAEAKRMVGRTPANIIAIRPLRDGVISDFDVTEQMIRYFVDKVHDRIGLLP
                     RPRMIIGIPSGVTEVEKRAVLDAALNAGARWARLIEEPMAAAIGAGLPVSEPSGSLIV
                     DIGGGTTEVAVISLGGIVVSRSIRIGGDEMDVDIVNFARREYNLYLGERTAEDIKIAI
                     GSAYPGEWDSQRVNLRGRDLLTGLPRSVEVGADQIREAIEPSVQQIVDTIRDTIEETP
                     PELVADIMDQGIVLAGGGALLAGLDRRVAEATQMPVHVADDPLTCVVRGTGEVLQDLD
                     RLSRVLVTETYSRPLR"
     gene            1430..2467
                     /locus_tag="VIF63_02095"
     CDS             1430..2467
                     /locus_tag="VIF63_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238201.1"
                     /translation="MLLVRSLLVGVIAIAVIGCGRGTSAPSAAPTPSLAAPTAAALAP
                     LSQLKLQATIPESMGASEILATDDALWALIHEDALLIRIDPATNEITGKVQLEPGYAY
                     RLGLAGGRLWTFHQLAGKVIGVDPVTMKVVASVSVGEQGDLFWVGDGAAWLIHGGDLN
                     RVDGKTAKLSSTPLPDCGIYDAAAGGGSIWFATGGGGLCQVDEQTGAIVKEATGAWHG
                     TGLAVVDGAVWLPGADNGLAIVDPTTLKITTAVPPPASGTVDGATYSIGTPGGDNTVV
                     FADSAGQTGWLRYTGATIGRVDLGADPAIVLYAGLLPGLYASNVTEAFGSLWVTNLHA
                     GEIQRFEVPAP"
     gene            complement(2479..2853)
                     /locus_tag="VIF63_02100"
     CDS             complement(2479..2853)
                     /locus_tag="VIF63_02100"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8238202.1"
                     /translation="MPPTRSDTLDTERLRQFKAEFFKALAHPLRIRILELLRGGSLSV
                     TQIQAATGAPGSSVSQQLAVLRNRNVVATERHGTTIIYRVADAELFELLDTARRIFNA
                     HLTDSVGLLRLVEEEQAVEARR"
     gene            2955..4955
                     /locus_tag="VIF63_02105"
     CDS             2955..4955
                     /locus_tag="VIF63_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8238203.1"
                     /translation="MSVGSALLATVAVLLALSVLSALAPHSRWNPRSLALILAAVASV
                     AIVALGLIVIAAEPISAAAGNVIGFAVIHVRYDSLSGLFLATLGIVGIASSIYGIGYE
                     AREGHRTADRTRAAYPVFLASLAVVFGADEALSFLLAWELMALTSAILVVGARPSPEV
                     ARTGYLYLALTHVATAALVVGFAVLADTAGSTDFAGFGAAAAALPPGARSIVFLLLLV
                     GFGTKAGAIPLHVWLPRAHPVAPSHVSALMSGVMIKAGIYGVVRFGIDILGPGPEWWG
                     GLVLAVGVVSAVLGVLYALMEHDLKRLLAFHSIENIGIILIGVGVGMVASSAGVPTLA
                     QLALTAALFHTLNHGVFKAVLFLAAGSIETAARTRDLNVLGGLARSMPLTAIAFGIGA
                     AAISGLPPLNGFASEWLTFQSLISAGGTEALSLPARAAVLVGVAGLALTTALAVACFV
                     KATGMTFLARPRGQGAAEAREAGPTMATTMAGLAALCIVLGLAAGPVAGHIGGIAATA
                     LGLPERAVEVAGPVAGMAAVYAAAPIALLLAAVAGVTWFVGLRGRRVRLVPTWTCGIA
                     PEAAFQYTATSYAKLIRLYFGPILRPAREVRVELHPGTPFPRTVRYEGNVRHVIDDRL
                     YVPLHAAGVAAAQLIRRLQSGSLQMYLAYTVAAVVVLLVVAR"
     gene            4955..5923
                     /locus_tag="VIF63_02110"
     CDS             4955..5923
                     /locus_tag="VIF63_02110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY8238204.1"
                     /translation="MPDQPFGEVAAIALGGIQAATTLLLAPLLFGITKRVKARLQYRR
                     GPALLQPYRDLAKWWAKESLESDVASPLSGAAPAIVLAATVVAALLVPLLGVRPPLDG
                     WGDLLVIVGLLALARFILALAALDAGGAFGGMGSSREVAITTLVEPALLLALAGAALA
                     AGSTDLGSIAAAGHAAGLAWFTPALILSAAAFAIVAVAETGHEPVDNPDTHLELTMIH
                     EGMLLESSGPRLAILMYSAELKVVVVVGVFSAAFFPIGLAATITPVTIVVAAGACLLK
                     LVAAAVILGILDATLAKIRILALPGLLLTATIIGAVGLAARLWLPV"
     gene            5924..6586
                     /locus_tag="VIF63_02115"
     CDS             5924..6586
                     /locus_tag="VIF63_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238205.1"
                     /translation="MFDLTTTLAVVDACAAGIVVLGVGMAATRSIGRSIWLVAGQSVL
                     TGVAALAVGVGTSTGHLVVGGFLAIAIKGFVIPLVLGSVLRRSPIRRERHLLIDRRAS
                     LVAAVAIVFIASNVVSGAVPGASLSASRALPAAIAEVLTGLLIVMTRRKALSLVVGLL
                     VFENGIALTAFSLTYGMPLVVEMGVLFDVLIVVVVAWVYAGRMLEVLGSTSTDRLRNL
                     RG"
     gene            6583..>7963
                     /locus_tag="VIF63_02120"
     CDS             6583..>7963
                     /locus_tag="VIF63_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8238206.1"
                     /translation="MTELLVVITIGGPVLAGALAWFAEPRRAAVAAVLGLGAAIAAGV
                     GLAVLVFSGGPARAFDGLVVVDALGAYVLVLVLAVAALSGAGSPAHLAHERAAGALRP
                     HDPGRYYALLLWFAAGLAAVPLLDNLGLVWVAIEATTIVSALLVGFSRTSAAIEAAWK
                     YLILGSIGIGFALLGTLLLYASSVGVLGETSDALAWTRIRGIAPALDPSLVRLAFLFA
                     LAGYGTKAGLVPFHTWLPDAHSEAPSPVSALLSGAALAVALYALARFQLVTSAAIGPA
                     FASTLLVAFGLVSLGVALPFIVAQGDLKRLLAYSSIEHIGLATLALGFGGHLALLGFT
                     LHLVSHGLAKATAFMAAGQLVAERGSRRIGRLAGSLARAPAHGRALLVATLILAGLPP
                     SAIFIAEIAILVGGVQSGWGIAAGIAAALLALAVAGLLFHVMRVAVGGASAADAEMEP
                     VSQANPRRAA"
BASE COUNT          985 a         2818 c         2771 g         1389 t
ORIGIN      
        1 ggtctccggc aaagccggtg acggcgacct ggacgggcct gaacgcgaac gcgatgccgt
       61 gctggagatc gcgcacgagc gggttcgcgg agatcgtcag caggatcagc gaggcgacga
      121 cgagcaccac gtacgcgacg cccttgcgcc ggagggattt gttggcgatg agggcgtcca
      181 tccgccgggt gtccctagcg cagcgggcgc gagtaggtct cggtcaccag gacgcggctg
      241 agtcgatcca ggtcctggag gacctcgccg gtgccgcgga cgacgcaggt gagcggatcg
      301 tcggcgacat ggaccggcat ctgggtcgcc tcggcgacac gccggtcgag gcctgcgagc
      361 agcgcgccgc caccggccag gacgatgccc tggtccatga tgtcggcgac gagctcgggc
      421 ggcgtctcct cgatcgtgtc gcggatcgtg tcgacgatct gctggacgga cggttcgatg
      481 gcctcgcgga tctggtccgc cccgacctcg acgctgcgcg gcaggccggt cagcaggtcg
      541 cggccgcgca ggttcacgcg ctgcgagtcc cactcgccag ggtacgcaga accgatcgcg
      601 atcttgatgt cctcggccgt gcgctcgccg aggtacaggt tgtactcgcg ccgggcgaag
      661 ttgacgatgt cgacgtccat ctcgtcgccg ccgatgcgga tgctccggct gacgacaatg
      721 ccgccgaggc tgatcaccgc gacctcggtc gtgccgccgc cgatgtcgac gatgaggctg
      781 cccgaaggct cgctcaccgg caggcccgcc ccgatcgcgg cggccatggg ctcctcgatc
      841 aggcgcgccc agcgcgcacc ggcgttgagc gcggcgtcga gcacggcgcg cttctcgacc
      901 tcggtcacgc ccgacgggat gccgatgatc atccgtggcc ggggcagcag cccgatccgg
      961 tcgtggacct tgtcgacgaa gtaccggatc atctgctcgg tcacgtcgaa gtcgctgatg
     1021 acgccgtcgc gaagcggccg gatggcgatg atgttcgccg gcgtgcgccc gaccatccgc
     1081 ttcgcctcgg cgccgattgc cagcacccgc ttcgacttcg cctcgatcgc cacgaccgac
     1141 ggctcgctga tcacgatccc gcggtcgcga acatggacga gggtgttcgc ggtgccgagg
     1201 tcgatcccga tgtcgcggga gaacaggccg agaattcggt cgagcattcg gtggaaggtc
     1261 cccagtcggg cgcgtccgcg cgggaattga gcgcggggaa cggcagtata gccgcggtct
     1321 cccgatctcc tgccgcagcc tgtccattgt gcgcagccgg cgcatactcg ccgcccgaca
     1381 ccgaggcgtg cccagccctc atcttgccgg taaggctatg aggcgcgacg tgttgctggt
     1441 tcgatcattg ctcgtcggtg tcatcgcgat cgccgtcatc ggctgcggcc gggggacgag
     1501 cgccccatcg gcggcgccga ctccttctct ggcagcgccc accgcggcag ccctggcgcc
     1561 gctgagccag ctgaagctcc aggcgacgat cccggagtcg atgggcgcct cggagatcct
     1621 cgcaaccgat gacgccctgt gggcgctcat tcacgaggat gccctgctga tccgcatcga
     1681 cccggcgacg aacgagatca ccgggaaggt ccagctcgag ccgggctacg cgtaccggct
     1741 gggcctcgcc ggaggccggc tgtggacctt tcaccagctc gcggggaagg tgatcggcgt
     1801 cgatcccgtg acgatgaagg tcgtcgcgag tgtgagcgtc ggggagcagg gcgacctctt
     1861 ctgggtcggc gacggtgccg cgtggctgat ccacgggggc gacctcaacc gggtcgacgg
     1921 caagaccgcc aagctcagct cgacgccgct gcccgactgc ggcatctacg acgccgcggc
     1981 cgggggcggc tccatctggt tcgcgaccgg gggcggcggc ctgtgccagg tcgacgagca
     2041 aaccggtgcg atcgtcaagg aggccaccgg cgcgtggcac ggcaccggtc tcgcggtcgt
     2101 cgatggcgcc gtctggctcc ccggagccga caatggcctc gccatcgtgg accccacgac
     2161 gctaaagatc accacggccg tgccgccgcc ggcaagcggg acggtcgatg gcgcgaccta
     2221 ttccatcggc actccgggag gcgacaacac ggtcgtcttc gccgacagtg ccggtcagac
     2281 cggctggctc cgctacacgg gggcgacgat cggtcgcgtg gacctcggcg ccgacccggc
     2341 aatcgtcctg tatgcaggcc tcctgccggg cctctacgcc agcaacgtca ccgaggcctt
     2401 cggttcgctg tgggtcacga acctccatgc cggggagatc cagcgcttcg aggtgccggc
     2461 gccgtagcgg cggccggcct atcggcgggc ctccacggcc tgttcttcct cgacgaggcg
     2521 gaggaggccg accgaatcgg tgagatgggc gttgaagatg cgccgtgccg tgtcgagcag
     2581 ctcgaacagc tcggcgtcgg cgaccctgta gatgatcgtg gtcccgtggc gctcggtcgc
     2641 gacgacgttc cggttgcgca gaaccgcgag ctgctgggac acggacgaac caggggcacc
     2701 cgtcgccgcc tggatctgcg tcacgctgag cgagccgcct cggagcagct caaggatgcg
     2761 gattcgcagc gggtgcgcaa gcgccttgaa gaactcggcc ttgaactggc gcaggcgttc
     2821 ggtgtcgagg gtgtcgctcc gcgtcggtgg catcccgttc cggtacctcg ctggtgggtg
     2881 gtcgggtcgt gcgacaagcg tagtgctgcc acagtattct gcaatcttcc gggattacgg
     2941 aggatgacag cggcgtgtcg gtcggcagcg cactcctcgc gaccgtcgcg gtcctcctcg
     3001 cgttgtccgt tctctcggcc ctggccccgc actcgcgttg gaacccccgc tccctcgcgc
     3061 tgatcctcgc ggcggtcgca tcggtggcga tcgtggcgct cggcctgatc gtgatcgccg
     3121 cggagccaat ctcggcggcg gccggcaacg tgatcggctt cgccgtgatt cacgtccgct
     3181 acgacagcct gtccgggctg ttcctcgcga cgctgggcat cgtcggcatc gcgtcgtcga
     3241 tctacggcat cggctacgag gcacgcgagg gtcacagaac agccgatcgc actcgggcgg
     3301 cctatcccgt cttcctcgcg agtctcgcgg tcgtgttcgg cgccgacgag gcgctctcgt
     3361 tcctcctggc gtgggagctc atggcgctga cctcggcgat cctggtcgtc ggcgcccggc
     3421 caagccccga ggtcgcccgt accggctacc tctatctcgc gctcacgcac gtcgccaccg
     3481 cggcgctcgt cgtcggcttc gcggtcctcg ccgacacggc gggctcgacc gactttgccg
     3541 gcttcggcgc ggcagcagcc gcgctcccac ccggcgctcg gagcatcgtg ttcctgctgc
     3601 tgctcgttgg tttcggaacg aaggccggag ccatcccgct ccacgtctgg ctgccgcggg
     3661 cgcatccggt ggcgccgtcc catgtgtccg cgctgatgtc gggcgtgatg atcaaggccg
     3721 gcatctatgg ggtcgtgcgg ttcggcatcg acatcctcgg gccgggcccg gaatggtggg
     3781 gcggactcgt gctggccgtc ggtgtcgtct cggcggtgct cggggtgctg tacgccctga
     3841 tggagcacga cctcaagcgg ctcctcgcgt tccacagcat cgagaacatc gggatcatcc
     3901 tcatcggggt cggcgtcggg atggttgcct cttcggccgg cgtgccgacc ctcgcccagc
     3961 ttgcactcac ggcggcgctt ttccacacgc tgaaccacgg cgtgttcaag gccgtcctct
     4021 tcctcgccgc gggctcgatc gagacggccg cacggacccg cgacctgaac gtcctcggcg
     4081 gcctcgcccg gtcgatgccg ctgaccgcga tcgccttcgg catcggcgcc gcggcgatca
     4141 gcggcctgcc gccgctcaat ggcttcgcaa gtgagtggct caccttccag agcctcatca
     4201 gtgccggcgg cacggaggct ctgtcgctgc ccgcccgcgc tgcggtcctg gtcggagtcg
     4261 ctggcctggc gctcacgacc gcgcttgccg tcgcctgctt cgtcaaggca acgggcatga
     4321 ccttcctcgc ccggccccgc gggcagggcg ccgccgaggc ccgggaggca ggccccacca
     4381 tggccacgac gatggccggc ctggcggccc tgtgcatcgt gctcggactt gctgcgggcc
     4441 cggtcgccgg ccacatcggt ggcatcgcgg cgaccgccct cggcctgcct gaacgcgcgg
     4501 tcgaggtggc cgggccagtc gccggcatgg ccgccgtcta cgctgccgcg ccgattgcgc
     4561 tcctgctggc agccgtcgcc ggcgtgacct ggttcgtcgg attacgtggt cgccgggtgc
     4621 ggctggtgcc gacctggacg tgcgggatcg ctcccgaggc ggcattccag tacacggcaa
     4681 cgagctacgc gaagctgatc cgcctgtact tcgggccgat cctgcgaccg gctcgggaag
     4741 tccgggtcga gctccaccct ggcacaccgt tcccgaggac ggttcgctac gaaggcaatg
     4801 tgcgccacgt gatcgatgat cggctctatg tgccgctcca cgcggccggg gtcgccgccg
     4861 cccagctgat ccgccggctc cagtcgggga gcctgcagat gtatctcgcc tataccgtcg
     4921 cggcggtcgt tgtcctcctg gtggtcgcgc gctgatgccc gatcagccct tcggcgaggt
     4981 cgcggccatc gcgctggggg gcatccaggc ggcgacgacg ctgctcctcg cccccctctt
     5041 gttcgggatc acgaaacgcg tcaaggcccg cctgcagtac cggcgcggcc ctgcccttct
     5101 ccaaccgtat cgggaccttg cgaagtggtg ggcaaaggaa tcgctcgaga gcgatgtcgc
     5161 gagcccgctg agcggcgcgg cgccggccat cgtgctggcg gcgaccgtcg tcgcggccct
     5221 gctcgtgccg ctcctggggg tgcgcccccc actcgacggc tggggcgacc tgctggtgat
     5281 cgtcggcttg ctggcgctgg cgcgcttcat ccttgcgctg gccgcgctcg acgccggggg
     5341 tgccttcggc ggcatgggca gcagtcgaga ggttgcgatc acgacgctcg tcgagccggc
     5401 cctcctgctc gccctcgccg gtgccgcgct ggcggcaggt tcaaccgacc tcgggtccat
     5461 tgccgccgcc gggcacgccg caggccttgc gtggttcacc cctgcgctga tcctctcggc
     5521 cgccgcgttc gccatcgttg ccgtggccga aaccgggcac gagccggtcg ataacccgga
     5581 cacccacctc gagctcacga tgatccacga gggcatgctg ctcgagtcct cgggcccgag
     5641 gctggcgatc ctcatgtatt cggcggagct caaggtcgtc gtcgtcgtcg gggtgttctc
     5701 ggccgcgttc ttccccatcg gccttgccgc gaccatcacc ccggtgacga tcgtcgtggc
     5761 cgctggcgcc tgcctgctga agctcgtcgc ggccgcggtc atcctcggga ttctcgatgc
     5821 aaccctcgcc aagatccgaa tcctggcact gcccgggctg ctcctcaccg ccacgatcat
     5881 cggcgcggtc gggctcgccg cccgactctg gctgcccgtc tgaatgttcg accttacgac
     5941 gacccttgcc gttgtcgatg cgtgcgcggc cggcatcgtc gtccttggtg tcggtatggc
     6001 cgcgacacgc tcgatcgggc gatcgatctg gctggtcgcc ggccagtcgg tgctgaccgg
     6061 ggtcgctgcc ctggcggtcg gtgtcgggac gagcacgggg cacctggtcg tcggtggctt
     6121 cctggccatc gcgatcaagg gcttcgtcat cccgctcgtg ctcgggtcgg tcctgcgacg
     6181 ctcgccgatc cggcgagagc ggcacctcct gatcgatcgc cgggcatcgc tcgtcgccgc
     6241 cgtggcgatc gtgttcatcg cgagcaacgt ggtcagcggc gccgtgcccg gggcgagcct
     6301 cagcgcgagt cgcgcgttgc ccgccgcaat cgccgaggtc ctgacggggc tgctcatcgt
     6361 gatgacccgc cgcaaggcgc tgtcgctcgt cgtcggcctg ctcgtcttcg agaacggcat
     6421 cgccctgact gcgttctcgt tgacgtacgg gatgccgctc gtcgtggaga tgggcgtgct
     6481 gttcgacgtc ctcatcgtgg tcgtcgtcgc gtgggtctac gcgggccgca tgctcgaggt
     6541 gcttgggagc acgtctaccg accggctgcg gaacctccgc ggatgaccga gctccttgtc
     6601 gtcatcacga tcggcgggcc cgtgctggcg ggcgcgctgg cctggttcgc agagccgcga
     6661 cgagccgctg tcgcggccgt cttgggcctt ggggccgcca tcgctgccgg cgtgggcctc
     6721 gccgtcctcg tcttcagcgg cgggccggcg cgcgcgtttg atgggctggt cgtcgtcgat
     6781 gcccttggcg cctacgtgct ggtgctcgtc cttgccgtcg ccgcgctctc gggcgccggc
     6841 tcgccggcgc atctggccca cgagcgcgcg gctggcgctc tgcgtccgca cgatcccggg
     6901 cgctactacg cgctcctgct gtggttcgcc gccggactcg cggcggtgcc gctcctcgac
     6961 aatctcggcc tggtctgggt ggcgatcgag gcgaccacga tcgtgtccgc attgctcgtg
     7021 ggcttctcaa ggacgtcggc ggcgatcgag gcggcctgga aatacctgat cctgggctcg
     7081 atcggcatcg gcttcgcgct gctcggaacg ctgctcctgt acgcgtcgag cgtcggtgtc
     7141 ctgggcgaga cgagcgatgc cttggcttgg acccggatca ggggcatcgc gccggccctc
     7201 gacccgagcc tcgtccgcct ggcgttcctg ttcgccctcg ccggctacgg aacgaaggcg
     7261 gggctcgtcc cattccacac atggcttccc gacgcccaca gcgaggcacc gagccccgtc
     7321 tcggcgctcc tgtccggggc agcactcgcc gtcgcgctct acgccctcgc gaggttccag
     7381 ctcgtcacgt cggcagcgat tggtccggca ttcgcgtcga cactcctcgt tgcattcggc
     7441 ctggtgagcc tgggtgtcgc actgccgttc atcgtcgccc agggcgacct gaaacgactg
     7501 ctcgcctact cgtcgatcga gcacatcggg ctggcgacgc tggcgctcgg tttcggcggc
     7561 catctggcgc tcctcggctt caccctccat ctcgtgagcc acggcctggc gaaggcgacc
     7621 gccttcatgg ccgcgggcca gctggtggcc gagcgcggca gccgccggat cggccgtctt
     7681 gccggctcgc tcgcccgggc gccggcgcac ggccgggccc tcctcgtcgc gaccctgatc
     7741 cttgcggggc tcccgccgtc ggcgatcttc atcgccgaga tcgcgatcct ggttggcggc
     7801 gtccagtcgg gctggggcat cgctgcgggc atcgcggccg cgctcctcgc cctggctgtc
     7861 gccgggctgc tgttccacgt gatgcgcgtc gccgtcggcg gggcaagtgc agccgacgcg
     7921 gagatggagc ctgtgtccca agccaaccct cgacgtgcgg ctg
//
LOCUS       DATBSW010000080         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_217526, whole genome shotgun sequence.
ACCESSION   DATBSW010000080 DATBSW010000000
VERSION     DATBSW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217526"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..>2533
                     /locus_tag="VIF63_02125"
     CDS             296..>2533
                     /locus_tag="VIF63_02125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8238207.1"
                     /translation="MRTYAPAGTEAVLERLLEEPSLAAAVKHHAHLPAREAITAPFPE
                     WLDPRIVAGLENRGITSLYSHQAEAVEAVHAGRDIVVVTPTASGKTLCYALPVLQALA
                     EDPSARALFLFPTKALGQDQVAEFAGLAKASSLAVAAATYDGDTPAPIRSAIRTAGQV
                     VVTNPDMLHSAILPHHTKWFQLFEQVRVIVIDELHTYRGVYGSHVANVLRRLLRLCAH
                     YGSNPIIVCSSATIGNPAELAAQLTGRTPLLIDRNGAPAGARHILLVDPPLLDAATGA
                     RGSALTHAERWALPFIRAGRQTIVFGRARVAVEIILTRLREALREHLGPRSRIRGYRG
                     GYLPTERRAIERGLRDGEILGVVSTNALELGVDIGALDVSILAGYPGSVAGTWQQLGR
                     AGRRQGVSVGVLIASGSAVDQYVINHPEFLLEGTPEEARIDPDNLHVLLAHLRCATFE
                     RPFEPGEVFGPGPVDDLLAFIGEEGHVRQAGDGRWYWSSENFPASEVSLRTAAPENVV
                     IIDTTPDRPRVIGETDLFSAQVLVHTQAIYLHDSTQYHVDKLDWGERKAYVRKVDVDY
                     YTYANRAVTLKPLDVFASEPAQGGRRIHGEVMVASLVTLFKKLKFGTDENVGWGPIDL
                     PELELQTTAYWLTAEDASVGWRKDDLDVALLGGGRAIQAVASVLLMVDPRDLGLVTQV
                     RSPHSEAPTIYLYEAVPGGIGLSERLFARHDELVVGAKALIEACGCDMGCPACTGPRL
                     DPAVNG"
BASE COUNT          366 a          898 c          882 g          387 t
ORIGIN      
        1 cggcgtccgc gccttgggat tccttctttg agccgccggc ggcgtgcgaa cggggctcgc
       61 ggaggaaggc cgggacagcg gacaggtcct tgccgcctcc ccctcggcgc gcggcggctc
      121 ggtcccgatc gggggctggc atcgtcgatc gagggtagct acgcgctcac gagggtgtca
      181 acccaactcc cgactggtgc gtgatcgggt tgtgacagcg gaggtggcac atcgtgcctt
      241 gacgctgtgg ctagactccg gtcaatcgaa catccgttct attggaggtc ctgccgtgcg
      301 cacttacgcc cccgccggca cggaggccgt gctggagcgg ctgctggagg agccgtcgct
      361 cgccgcggcg gtgaagcatc acgcccacct gcccgcccgc gaagcgatca cggcgccgtt
      421 ccctgaatgg ctggacccac gcatcgtcgc gggcctcgag aaccgcggca tcacctcgct
      481 gtacagccac caggcggagg cggtcgaggc ggtccatgcg ggccgggaca tcgtggtcgt
      541 cacgccgacc gcctcgggca agacgctgtg ctacgcgctg cccgtcctcc aggcgctggc
      601 cgaggacccg tcagcgcgcg ccttgttcct cttcccgacg aaggccctcg gccaggacca
      661 ggtcgccgag ttcgctggcc tcgccaaggc gagctcgctc gctgtcgcgg cggcgacgta
      721 cgacggcgac acgccagcgc cgatccgctc ggcgatccgc acggccggcc aggtcgtcgt
      781 gacgaacccg gacatgctcc actccgcgat cctcccgcat cacacgaagt ggttccagct
      841 cttcgagcag gtccgggtga tcgtgatcga cgagctgcac acctaccgcg gcgtgtacgg
      901 cagccacgtg gcgaacgtgc tgcgacgcct cctccggttg tgcgcgcact acggctcgaa
      961 cccgatcatc gtctgctcgt cggcgaccat cggcaatccg gccgagctcg ccgcgcagct
     1021 caccggccgc acgccgctgc tcatcgaccg gaacggtgcg ccggccggcg ccagacacat
     1081 cctgctcgtc gacccgccgc tgctggacgc ggccacgggt gcgcgtggct cggcgctgac
     1141 ccacgcggag cgttgggcgc tgccattcat ccgggccggc aggcagacca tcgtgttcgg
     1201 gcgcgcgcgt gtcgctgtcg agatcatcct gacgcggctg cgcgaggcgc tgcgcgagca
     1261 cctcggcccg cggtcgcgga tccgcggcta tcgaggcggc tacctgccga cggagcggcg
     1321 ggcgatcgaa cgcggcctcc gcgacggcga gatcctcggc gtggtctcga cgaatgccct
     1381 ggagctcggc gtcgacatcg gcgcgctcga cgtgtcgatc ctcgccggct acccggggtc
     1441 cgttgcgggg acgtggcagc agctcggccg cgcgggacgg cgccagggcg tcagcgtcgg
     1501 cgtgctcatc gcgtcgggct cggcggtcga ccagtacgtg atcaaccatc ccgagttcct
     1561 gctcgagggc acgcccgagg aggcccggat cgacccggac aacctgcatg tgctgctcgc
     1621 ccacctccgc tgcgcgacct tcgagcgccc gttcgagccg ggcgaagtat tcgggccggg
     1681 accggtcgac gacctgctcg cgttcatcgg cgaggagggc cacgtccgac aggcgggtga
     1741 cggacggtgg tactggagct ccgagaactt cccggcatcc gaggtcagcc tgcggaccgc
     1801 tgccccggag aacgtcgtga tcatcgacac cacgccggat cggccgcggg tcatcggtga
     1861 gacggacctg ttctcggcgc aggtgctggt ccatacccag gcgatctacc tgcacgacag
     1921 cacccagtac cacgtcgaca agctcgactg gggcgagcgc aaggcgtatg tccggaaggt
     1981 cgacgtcgac tactacacgt atgcgaaccg cgcggtgacg ctgaagccgc tcgacgtctt
     2041 cgcgagcgag cccgcgcagg gcggccggcg catccacggc gaggtcatgg tcgcgagcct
     2101 cgtcacgctg ttcaagaagc tcaagttcgg gaccgacgag aacgtcggct ggggcccaat
     2161 cgaccttccc gagctggagc tccagacgac ggcgtactgg ctgaccgccg aggacgcctc
     2221 ggtgggctgg cgcaaggacg acctcgacgt cgccctcctc ggcggtggcc gcgctatcca
     2281 ggcggtcgcg tcggtgctgc tcatggtcga cccgcgcgac ctgggtctcg tgacccaggt
     2341 tcggtcgcca cactccgagg cgccgaccat ctatctgtac gaggcggtgc ccggtggaat
     2401 cggcctgtcg gagcggctgt tcgcgcgcca cgacgagctg gtcgtcgggg caaaggcgct
     2461 catcgaggca tgcggttgcg acatgggctg ccccgcctgc accggcccac gccttgatcc
     2521 cgcggtgaac ggc
//
LOCUS       DATBSW010000081         9006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1169766, whole genome shotgun sequence.
ACCESSION   DATBSW010000081 DATBSW010000000
VERSION     DATBSW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169766"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VIF63_02130"
     CDS             complement(<1..860)
                     /locus_tag="VIF63_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238208.1"
                     /translation="MSLASAVVIGLGLLVPAALAAEPVTHSGRVVISTQGDITIPAGE
                     QADVVIVVQGNADIEGIVNTLVLVDGTANLTAARLETVVAVRSTVEVADGTVILGELQ
                     RLDSTVHQSGNVQIEGGITDISSWFVEAGAVLAPALILLWIGFGLSTVLAGLLLAAIA
                     SRQVREAEQLISRDPLMTFVVGFIGVIAIPVAAVLLLVTVVGAPFGLAVLFGALPILG
                     YAGYLVAAIWVGEWIVRSFGSGSRPDRPYLAAVIGVVTLGLVGLVPFLGLIVTVASLL
                     GFGALIRLGFR"
     gene            complement(869..1048)
                     /locus_tag="VIF63_02135"
     CDS             complement(869..1048)
                     /locus_tag="VIF63_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238209.1"
                     /translation="MKRFLAFALPILGTFLVLGLGVFLVRTQPEAIRTRFGALRETAT
                     SVAEEVAHEVKEVLE"
     gene            1279..1530
                     /locus_tag="VIF63_02140"
     CDS             1279..1530
                     /locus_tag="VIF63_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8238210.1"
                     /translation="MLETIPDTGGPLDIERDRCKGCELCVSVCPKHVLELDPGLVNAL
                     GHHPVRLTDAGACTSCALCVRVCPDSVFTVYARPKAVPA"
     gene            1527..2597
                     /gene="vorB"
                     /locus_tag="VIF63_02145"
     CDS             1527..2597
                     /gene="vorB"
                     /locus_tag="VIF63_02145"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEY8238211.1"
                     /translation="MIASPVLMKGNEAMADAAIEAGCDAYFGYPITPQAELLERMARR
                     MPELGRVFVQAESEIAAINMGLGAAATGRKVLVSTSSPGMSLMAETMSYLAGSELPIV
                     VINVMRGGPGLGSIGPSQSDYFQATRGHGHGDYRVPVLAPASIPEAVALVADAFELAC
                     RYRTPVIILADGVMGQAMEPVSPTFRKAAWDASAWELTGAAGRRPRVIRSLHLLPEDL
                     ERHNTHLQAKFTEIAAHEVRYEAVDLDDAEIGVVAYGTAARVARTAIDRARDVGLRAG
                     LFRPISLWPFPERELREAARHLRALVVVELSAGQMIEDVRLAVEGTKPVFFVGRTGGM
                     VPSPREVLEKVRMSWALTAAVA"
     gene            2594..3403
                     /locus_tag="VIF63_02150"
     CDS             2594..3403
                     /locus_tag="VIF63_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8238212.1"
                     /translation="MNAPLAMVEAPPRSSEARKVIYRRPDVLVDRSSHYCPGCGHGIV
                     TRLVAELLDELDLADRTIGVASVGCSVFAYDYLDVDFVESPHGRAPAVATGVRRARPD
                     QFVFTYQGDGDLAAIGTAEIIHAAARGERFSVIFVNNGIYGMTGGQLAPTSLLGQRTT
                     SSPLGRDARLAGFPLPITEMLALVPGVSYAARGSIADPGQIGRTKAMLRRAFEAQLTG
                     DGLSIVEVLSTCPVGWGMTAPEAMRHLSDDVVQTYPLGVLADRTGRGTPAP"
     gene            3400..3969
                     /locus_tag="VIF63_02155"
     CDS             3400..3969
                     /locus_tag="VIF63_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY8238213.1"
                     /translation="MILSTILSGFGGQGLLFAGQVLAQAALDEGNEVTWLPSYGPEMR
                     GGAASCTVIVSDRPIGSPIADAADVLIALNPPSLARHQALVAPGGLIVLNSTLVETVA
                     RADVEVVRVPCSALARDAGDDRAVSIVALGAGIARRPVVAPDSIRGALRKLAAKAGPE
                     TVERNLRAFDAGFAGALEAAPPELAGATA"
     gene            complement(3992..5695)
                     /gene="maeA"
                     /locus_tag="VIF63_02160"
     CDS             complement(3992..5695)
                     /gene="maeA"
                     /locus_tag="VIF63_02160"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HEY8238214.1"
                     /translation="MTSRGEPSADVATGVGRIVTERGRALIGQSLLNKDTAFTREERD
                     QLGLHGLLPARVSTIDEQVALELEHVRRKADDVERYIGLAALQDRNETLFYRVLSENL
                     EEFLPIVYTPTVGRACQDFSHMFRRPRGVWITPDDVDRMDEVLAGAGGASTRLIVVTD
                     NERILGLGDQGAGGMAIPVGKLALYSAAAGIYPALTLPVSLDVGTDRRELLDDPLYLG
                     YRKPRLRGDAYDRVVEAFVDSIGRVFPGAIVQWEDFKQHNALRILDRYRHRLPSFNDD
                     IQGTGAVVLGGLLAARRGAEALRRDRFLILGAGAAGIGIARTIERRLGPTPLGSPPRL
                     VLMDSHGLVHIERPDVQAHQLPFAVDPRWFATAGLAEGGLADPVAVARALGATVLIGT
                     TGTAGTFTEPLVREVARHARVPIVLPLSNPSDRAEARPVDIVAWTGGRALIATGSPAS
                     VPGGVTIGQANNVFIFPGVGLGALVAEAREVTDEAFVTAAEELAASSSRARLSTGAIY
                     PPIADLRPIARRIAIAVVRRLRDTGYGRQFRDEEIEPAVDRAMWTPAYVPYAPGPARR
                     V"
     gene            5943..6257
                     /locus_tag="VIF63_02165"
     CDS             5943..6257
                     /locus_tag="VIF63_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238215.1"
                     /translation="MEIAFSIGFGGWIVVIAGALIFGFVAQSVGEARTGYEWLVDGVA
                     AFAGAVIVSEFIISLRTFEPVYDGLALVPALIGGLALGLVIEVATRYVTGGKYTARPM
                     AV"
     gene            6738..7685
                     /locus_tag="VIF63_02170"
     CDS             6738..7685
                     /locus_tag="VIF63_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8238216.1"
                     /translation="MTPRPGLTRIPQTLRWGIVLAVATAVISGFSIFVNAFAVKQLPD
                     PAVYTTLKNGVAALLLLALAAMSVKPESVRAVPKRGWGWLAAIGVIGGSVPFLLFFTG
                     LAQASAPSAAFIHKTLFVWVALLAVPFLGERLGKAQIGALLVLLASQLLIVPPAGITW
                     GTGETLIAAATLLWALETIVARRVLRTVPSGVVGAARLGVGLVVLVGYLAVTGKLATI
                     ATLTSGQLGWALLTGAILSAYVGTWFAALQRAPASLVTAILVLGAPITAGLQALQGGA
                     IPATPALVGHGLILVAGAALVMPALRRWQTAAIPAGVTA"
     gene            7682..8500
                     /locus_tag="VIF63_02175"
     CDS             7682..8500
                     /locus_tag="VIF63_02175"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEY8238217.1"
                     /translation="MTSAAAPGLLAVRGAPHVASAWARPIAPAPGPVRFARYAFGPNR
                     LGYCGPDEAGELFQQATVGRELPKLRELAAQFEGAYPYLCLIAESNGIADPLNAAVVE
                     AYWLGGGLLAKVRAKDFGASLEARFRSRLHGDGWRWLGSKPEVGATPNHAFHVLDVFP
                     RLGFMRSGEMDRVIQVMDSCRIRWGRVLERDGDSLVVSAVPLEMVDGKLQLGSPRVEH
                     VRGWVEGAGFVDDVVAGDVVSIHWDWACERLDAERLAGLQQSTSAELRLANLTI"
     gene            8600..8950
                     /locus_tag="VIF63_02180"
     CDS             8600..8950
                     /locus_tag="VIF63_02180"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8238218.1"
                     /translation="MDEITRLQADVLKVLASPLRLEILHLLADGPLEVSGIAAAIGAT
                     QPHASQQLSVLRTAGLVEADRVGREIRYGLADRDVIVACDVMRGVLQRRLARLARLAT
                     DQPIRPTIQLGRSM"
BASE COUNT         1333 a         3133 c         3163 g         1377 t
ORIGIN      
        1 cggaagccca ggcggatcag cgcaccgaag ccgagcaggc tcgccacggt cacgatcaag
       61 ccgaggaacg ggacgagccc gacgagaccg agggtgacga cgccgatgac ggctgcgaga
      121 tacggccgat cgggccgcga ccccgagcca aacgaacgga cgatccactc gcccacccag
      181 atggccgcga cgaggtagcc cgcatagccg aggatcggca gcgcgccaaa gaggacggcc
      241 agcccgaacg gggcgccaac gacggtcacg agcaggagca cagcggccac cgggatcgca
      301 atcacgccga tgaagccgac gacgaatgtc atcaacgggt cgcgcgagat gagctgctcg
      361 gcctcgcgca cctggcgaga ggcaatcgcg gcgagcagca acccggcgag gaccgtggac
      421 agtccgaagc cgatccacag caggatgagc gccggagcga gcacggcacc cgcctcgacg
      481 aaccagctcg agatgtcggt gatgccgccc tcgatctgga cgttcccgct ctggtgaacg
      541 gtcgagtcga gtcgctggag ctcaccgagg atcacggtgc cgtcggcaac ctcgaccgtg
      601 ctgcgcacgg ccacgaccgt ctccagccgt gccgcggtca ggttcgccgt cccgtccacg
      661 aggacgaggg tgttgacgat gccctcgatg tccgcgttgc cctggacgac gatcacgacg
      721 tcggcctgct cgccggcggg gatggtgatg tccccttgcg tcgagatcac gacgcggccg
      781 gagtgcgtca ccggttccgc cgcgagggct gccggcacca ggaggccgag gccgatgacg
      841 acggcgctgg cgagggacat gagtcgcctc attcgagcac ctccttgact tcgtgggcga
      901 cttcttcggc gacgctcgtc gcggtctcgc gaagggcgcc gaaccgagtc ctgatcgctt
      961 ccggctgggt ccggacgagg aagacaccga gcccgaggac gaggaacgtc ccgaggatcg
     1021 gcagcgcgaa ggccaggaaa cgcttcatgg aactacctcc gatgctgtgc gttgaaccac
     1081 cctctatcga agcgcacgcg cttcgtcgga gatagagcca tcggcacccg ggacacgggc
     1141 cgtcgggcac cgcggtgcga tcgagcgccg acggcaaggg cggcccccgc tgttcggccc
     1201 gaacggcccg gggatggcgg atcgttcggc acgtgcgatg cccgttggct gcgcctacgt
     1261 tcatggggag gtggtcgagt gctggaaacc attccggaca ccgggggacc gctcgacatc
     1321 gagcgcgacc ggtgcaaggg ctgcgagctc tgcgtgagcg tctgcccgaa gcacgtcctc
     1381 gagctcgacc cggggctggt caacgcgctc ggacatcatc cggtccggct gacggatgcc
     1441 ggcgcctgca cgagctgcgc gctctgcgtc cgcgtctgcc cggactcggt gttcacggtc
     1501 tacgcgcgcc cgaaggcggt cccggcgtga tcgcatcgcc ggtgctcatg aagggcaacg
     1561 aggcgatggc ggatgccgcc atcgaagcag gctgcgacgc ttacttcggc tacccgatca
     1621 caccgcaggc cgagctcctc gagcgcatgg cccgccggat gccggagctc ggccgcgtct
     1681 tcgtccaggc cgagagcgag atcgcggcca tcaacatggg gctcggcgca gccgcgacgg
     1741 gccggaaggt cctggtgagc acgtcgagcc cgggcatgag cctgatggcg gagacgatga
     1801 gctacctggc tggatccgag ctcccgatcg tcgtcatcaa cgtcatgcgc ggcgggcccg
     1861 gcctcggcag catcgggcca tcccagtccg actacttcca ggcgacccgc ggacatggcc
     1921 acggcgacta tcgcgtgccg gttctcgcgc cggcctctat tccggaggcg gtcgccctcg
     1981 tggctgacgc cttcgagctg gcctgccgat atcgaacgcc ggtgatcatc cttgccgacg
     2041 gcgtgatggg ccaggcgatg gagcccgtct ccccgacctt ccgcaaagcg gcctgggatg
     2101 cctccgcctg ggagctgacc ggggcggcag gacgccggcc gcgcgtgatc cgatcgctgc
     2161 atctcctgcc ggaggatctc gagcgccaca acacccacct ccaggcgaag ttcaccgaga
     2221 tcgccgcgca cgaggtccga tacgaagccg tcgacctcga cgatgccgag atcggggtcg
     2281 ttgcctacgg caccgccgcc cgggtcgcca gaaccgcgat cgaccgtgcc cgggacgtgg
     2341 gcctgcgcgc gggcctcttc cgcccaatct cgctctggcc gtttcccgag cgcgagctcc
     2401 gggaggccgc tcgccacctg cgcgcgctcg tcgtggtcga gctgtcggcc ggccagatga
     2461 tcgaggacgt ccgcctcgcg gtcgagggca cgaagccggt gttcttcgtg ggccgaaccg
     2521 gcggcatggt gccctcgccg cgcgaggtcc tcgagaaggt gcggatgagc tgggcgctca
     2581 ccgcggcggt cgcatgaacg cgccgttggc aatggtcgag gcaccgccgc gcagctcgga
     2641 ggcgcggaag gtcatctatc ggcgcccgga cgtgctggtc gatcgatcga gccattactg
     2701 ccccggctgc ggccacggca tcgtcacccg cctggtcgcc gagctcctcg acgagctcga
     2761 ccttgccgac cggacgatcg gcgtggcatc ggtcgggtgc agcgtgttcg cctacgacta
     2821 cctcgacgtc gacttcgtcg agtcgcccca cggacgggct cctgcggtgg cgaccggggt
     2881 gaggcgcgcc cggccagatc agttcgtctt cacctaccag ggcgacggcg acctggcggc
     2941 aatcggcacg gccgagatca ttcacgccgc cgcgcgtggt gagcggttca gcgtgatctt
     3001 cgtgaacaac ggcatctacg gcatgaccgg tgggcagctg gcgcccacat ccctcctcgg
     3061 ccagcggacg acatcgagcc cgctcggccg cgacgccagg ctggcgggct tcccgctgcc
     3121 gatcacggag atgctcgccc tcgtgccggg cgtctcgtat gccgcgcggg gctcgatcgc
     3181 cgacccaggg cagatcgggc gaacgaaggc catgctccgg cgtgccttcg aggcacagct
     3241 gaccggtgac ggcctctcga tcgtggaggt cctgtcgacg tgcccggtcg gctgggggat
     3301 gaccgcgccc gaggcgatgc gccacctgtc cgatgacgtc gtgcagacgt atcccctggg
     3361 cgtgctcgcc gaccgtaccg gccggggaac cccggcccca tgatcctgtc gaccatcctc
     3421 agcggctttg gcggacaggg cctcctgttc gccgggcagg tccttgccca ggcagcgctc
     3481 gacgagggca acgaggtgac ctggctgccg tcctacgggc cggagatgcg aggcggggct
     3541 gcctcgtgca cggtcatcgt gagcgaccga ccgatcggct cgccgatcgc cgacgccgcg
     3601 gacgtgctca tcgcgctgaa cccgccgtca ctcgcacgcc atcaggccct cgtcgccccc
     3661 ggcgggctca tcgtcctgaa cagcacgctc gtcgagacgg tggcgcgggc cgacgtcgag
     3721 gtggtacggg ttccgtgctc cgcgctggcc agggatgccg gcgacgatcg ggccgtgagc
     3781 atcgtcgcgc tcggcgccgg gattgcccgg cgtcccgtcg tcgcgccgga ctcgatccgt
     3841 ggagcgctgc ggaagctggc tgcgaaggcg ggtcccgaga cggtcgagcg caacctccga
     3901 gcgttcgatg ccggcttcgc gggggcgttg gaggcagcgc cgccggagct ggccggggcg
     3961 acggcctgag cctcggacag gtgtccgcgg gtcagacccg gcgggcagga ccgggcgcgt
     4021 aggggacgta cgcaggggtc cacatggcgc ggtccacggc gggctcgatc tcctcatccc
     4081 ggaactggcg gccgtagccg gtgtcgcgca gccgccgcac gaccgcgatc gcgatccgcc
     4141 gcgcaatcgg gcgcaggtcg gcgatgggcg gatagatcgc gcccgtcgag agacgcgctc
     4201 gtgacgagga ggcagcgagc tcctcagccg ccgtgacgaa tgcctcgtcg gtcacctcac
     4261 gcgcctcggc gacgagcgca ccaagcccga cccccgggaa gatgaagacg ttgttcgcct
     4321 gtccgatcgt cacgcctccc ggcaccgagg ccgggctgcc ggtcgcaatg agcgcccgac
     4381 cacccgtcca ggcgacgatg tcgaccggtc gcgcctccgc acgatcgctc ggattggaca
     4441 ggggcagcac gatgggcacc cgagcatgcc tcgcgacctc gcgcaccagt ggttcggtga
     4501 acgtcccggc agtcccggtc gtgccgatca ggaccgttgc gccgagtgct cgggcgaccg
     4561 cgaccgggtc ggcgagcccg ccttcggcca gcccggccgt ggcgaaccat cgagggtcga
     4621 cggcaaaggg cagctggtgc gcctggacgt ccggccgctc gatatgcacc agcccgtggc
     4681 tatccatgag cacgaggcgg ggcggggacc ccaatggcgt ggggccaaga cgtcgttcga
     4741 tcgtccgggc gatgccgatg ccggcagcgc cggcgccgag gatcaggaac cgatcccggc
     4801 ggagcgcctc ggcgcctcgc cgagcagcca gcaggccgcc cagcacgacg gcgccggtgc
     4861 cctggatgtc gtcgttgaag ctcggcagcc gatggcggta ccgatcgagg atccgcagcg
     4921 cgttgtgctg cttgaagtcc tcccactgga cgatggcccc gggaaagacg cggccaatgg
     4981 agtcgacgaa ggcctcgacg acccggtcgt aagcatcgcc gcgcaggcgt ggcttccgat
     5041 agccgagata cagcggatcg tcgagcagct cgcggcggtc ggtacccacg tcgagggaca
     5101 ccggcagcgt gagagccggg tagatgcccg cagctgcgct gtacagcgcc agcttgccga
     5161 ccgggatggc catgcctccg gcgccctggt cgccgagacc gagaatccgt tcgttgtcgg
     5221 tcacgacgat gagccgcgtc gatgccccgc cggcgcctgc gaggacctcg tccatccggt
     5281 ccacgtcgtc cggtgtgatc cacacgccgc gcggccggcg gaacatgtgg ctgaagtcct
     5341 ggcacgctcg cccgaccgtc ggggtgtaga caatcggcag gaactcctcg aggttctcgc
     5401 tcaggactcg atagaacaac gtctcatttc ggtcctggag cgctgccagg ccgatgtatc
     5461 gctcgacgtc atcggccttt cgacgaacgt gctcgagctc cagtgcgacc tgctcgtcga
     5521 tcgtgctcac ccgcgccggg aggagcccat gcaggccgag ctgatcgcgc tcttcgcgcg
     5581 tgaacgcggt gtccttgttg agcagcgact ggccgatcaa cgcccgcccg cgttcggtca
     5641 cgatgcgtcc gacgccggtg gcaacgtcgg ccgacggctc gccgcgcgat gtcatcgacc
     5701 acctccgaga cgtacggcag atcgccgcgg agctcagcct cagcctgcgt ggatcgagtg
     5761 gacagggccg tcaggcccgt gcccggcagg tcagccggcc tgaagccggg ccagcaagtg
     5821 tccggtccgc ggggatagtc ggtctggctg tccacttcgg gagcctcggc tctaccggca
     5881 agtcgcctgg tcagggagga ttgtcgcgag gctctgatca agcgccaaag gagggtgcac
     5941 caatggagat tgccttcagc attggtttcg gtgggtggat cgtggtcatc gcgggcgccc
     6001 tgatcttcgg cttcgtcgcg cagtccgtgg gcgaggcgcg gaccggctac gagtggctgg
     6061 tcgacggcgt cgccgcgttc gcgggcgccg tgatcgtgag tgagttcatc atctcgctcc
     6121 gcacgttcga gcccgtctac gacggcctcg cgcttgtgcc ggccctcatc ggcggcctcg
     6181 cgctcggcct cgtgatcgag gtggcgacgc ggtacgtgac gggtgggaag tacacggcgc
     6241 ggccgatggc cgtctgaagt ccgtccccat ccgtgggcca ggtgacccgc cggcgcaagt
     6301 cggcgggtca ccctttgccc ttcgtagggc cggatggctg ggccgggata ggcactccgg
     6361 caggacctgc gtgacgctcg gccctccctc gtgaggggcg tccgggtcga gagtgacgcc
     6421 atgagccact cgattcacag ccgcctgcgc gacgaccggg tctgaccgat gtgcatcgcg
     6481 tatccagccc gtatcaccgc cctcgacagc gacgctgtcg tggtcgacat ggccggccgc
     6541 acccgccggg cgtccctgct catgatgccg gaggtccagg ttggggcgtg ggttctcgtg
     6601 gctgccggga gcgtcgtccg ggagatcgac gccgccgaag ccgcggacct cggagcccga
     6661 ctcgcggcgg cgatggccgc caccacgcaa gcgcctcacg acgacgccat ccagcccaca
     6721 cgcccaggag gtatgacatg acgccccggc ccggtctcac ccgaatcccg cagacgctcc
     6781 gctggggcat cgtgcttgcg gtcgcgacag ccgtgatcag cggattctcg atcttcgtga
     6841 acgcgttcgc ggtgaagcag ctccccgacc ctgccgtcta cacgaccctg aagaacggtg
     6901 tggcggcgct cctcctgctt gccctggcgg caatgagcgt caagccggaa tccgtgcgcg
     6961 ccgttcccaa gcgtggctgg ggctggctcg ccgccatcgg cgtgatcggc ggcagcgtgc
     7021 cgttcctgct cttcttcacc gggctcgccc aggcaagcgc gccgagcgca gcgttcatcc
     7081 acaagaccct cttcgtctgg gttgccctcc tcgccgttcc cttcctcggt gaacgccttg
     7141 gcaaggcaca gatcggcgcg ctgctcgtcc tgttggccag ccagctcctg atcgtgccgc
     7201 ctgcgggcat cacctggggc accggagaga ccctgatcgc ggcggcgaca ctgctctggg
     7261 cgctcgagac catcgtggcc cggcgcgtcc tgcgaaccgt cccgtcgggc gtcgtgggcg
     7321 cggcgcgcct cggcgtcggt ctcgtcgtcc tcgtcggcta tctcgccgta accggcaagc
     7381 tcgccaccat tgccacgctg acctccgggc aattgggttg ggcgctgctg acgggtgcaa
     7441 tcctgtccgc atacgttgga acctggttcg ccgcgctcca gcgcgcgccg gcaagcctcg
     7501 tgaccgcgat cctggtcctc ggggcgccca tcacggccgg tctgcaggcg ctccagggcg
     7561 gcgcaattcc ggcgacgccc gcgctcgtcg gccatggcct gatcctcgtc gcgggtgctg
     7621 cactcgtgat gccggcgctc cgacgctggc agacagccgc gatcccagcg ggcgtcacgg
     7681 cgtgacatcc gcggccgcac ccgggctgct cgcggtgcgc ggggcaccgc acgtcgcgag
     7741 cgcctgggca cggccaatcg ctccagcgcc tgggccggtc cggttcgcgc gctatgcctt
     7801 cggcccgaac cgcctgggct actgcgggcc ggacgaggcc ggtgagctgt tccagcaggc
     7861 cacggtcggg cgggagctgc cgaagctgcg cgagctcgcc gcccagttcg agggcgcgta
     7921 tccgtacctg tgccttatcg ccgagagcaa cggcatcgcc gacccgctca acgccgccgt
     7981 cgtcgaggcg tattggttgg gtggcgggct gctggcgaag gtccgcgcga aggacttcgg
     8041 ggcgtcgctc gaggcgcgtt tccgtagccg gctgcacggt gacggatggc gctggctcgg
     8101 ctcgaagccc gaggtcggcg cgaccccgaa tcacgccttc cacgtcctcg atgtattccc
     8161 ccgcctgggg ttcatgcggt cgggggagat ggaccgggtc atccaggtca tggattcctg
     8221 ccggatccgc tggggacgtg tcctcgagcg cgacggcgac tcgctcgtcg tctcggcggt
     8281 gccgctcgag atggtcgacg gcaagctgca gctcggcagc ccgcgcgtgg aacacgttcg
     8341 tggatgggtc gagggcgccg gattcgtcga cgatgtcgtc gccggcgacg tcgtgtcgat
     8401 ccattgggat tgggcgtgcg agcggctcga cgccgagcgc ctcgcgggac tccagcagtc
     8461 gacgtcagcg gagcttcggc tcgcgaacct gaccatctga cgggtcgggc cggacggccc
     8521 gcgatcgggc gcgaatggcc cgacggattg ggcccgctcg gcactcatgc gcaaacgtgc
     8581 atgcgtccat gctcgggtca tggatgagat caccaggctc caggcagacg tcctgaaggt
     8641 gctggcgagc ccactccggc tcgagatcct ccatctcctc gccgacgggc cgctcgaggt
     8701 gagcggaatt gccgccgcga tcggcgcgac ccagccgcac gcatcgcaac agctgtcggt
     8761 ccttcgaacc gcgggcctcg tggaggccga ccgggtcggg cgtgagatcc gatacggact
     8821 cgccgatcgg gacgtgatcg tggcgtgcga tgtcatgcgc ggcgtccttc agcgccgcct
     8881 cgcccgactt gcgcgcctcg ccacagacca gccgattcga ccgacgatcc agctcggtcg
     8941 ctcgatgtga ccgcctcgaa ggagcaccac gatggatgaa ccgctcaacc tggtcctctt
     9001 ctcggg
//
LOCUS       DATBSW010000082         9394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_147034, whole genome shotgun sequence.
ACCESSION   DATBSW010000082 DATBSW010000000
VERSION     DATBSW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147034"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VIF63_02185"
     CDS             complement(<1..1121)
                     /locus_tag="VIF63_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8238219.1"
                     /translation="MSGTHSPSADGQAAGLAPRTFQGRLTLAFVAVVAVTLGLVGLLV
                     VNRLGAYFDQQQEDDLASRARSVAQYVIVIAEGTSNVRAGRPVVSADGLVDPEIVTEL
                     QRSGQQRIIADQLAQADVRVTLGLANGTFQPAANGTFFAEELASPKPNQVQERMSSKP
                     VLEKVTSASLPYGVEVVLQDPFTYRAAAIQNVSGLLAVTGLIALAVAVVVAAMLTRRI
                     TTPLRRLTEATRDLAEGDFARRVPSEAIEDGPIELSELGLQFNTMAERLQESIGIIRS
                     DRDRGREFLADVSHELRTPIAALRTFNELLTEGAADDASTRQEFLETSRAQLERLDWL
                     AQNLLELSKLDSGLVLLDLRREDLRTSVEQAVDQAAATA"
     gene            complement(1093..1791)
                     /locus_tag="VIF63_02190"
     CDS             complement(1093..1791)
                     /locus_tag="VIF63_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8238220.1"
                     /translation="MAATILVVEDEHAVARGLQYALEQEGYEVTLARSGEEGLELATN
                     SAPDLVLLDVRLPGMDGFEVLRRLRAAGTKSPVLMLTARDEEVDKVIGLELGADDYLT
                     KPFGLRELLSRVKALLRRAYGDLADAVGGRVIRHRDLLIDLDRRRVQRGDRRISLTPT
                     EFEILRHLASKPGRVYTRAELLDLLRDYEALDQDEKTINVHVSHLRDKLEDDPTNPAF
                     VQTIRGVGYAFAER"
     gene            1849..2529
                     /locus_tag="VIF63_02195"
     CDS             1849..2529
                     /locus_tag="VIF63_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY8238221.1"
                     /translation="MSAAPARPKLAAASRTLTGKKVSQMRAAGRLPAVVFGRGIDSDS
                     VSVDTHEFEQLRRHAGSNTLIDLSVDGAAPAPVLVYGVQTHRVTRRPLHVDLYVVRMT
                     EELTVDVPLVSEGLSDAVENAAGTLLHVIDHVRVRALPDHLPESIHYSIESLRTFDDQ
                     IHVRDLEIPADATLITDGDEVVAKVLPPRVEEEPVVAEAVEGAEGEEGAEAAEGAEGA
                     ESSEGDEG"
     gene            complement(2553..3374)
                     /locus_tag="VIF63_02200"
     CDS             complement(2553..3374)
                     /locus_tag="VIF63_02200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8238222.1"
                     /translation="MSYDEIAEGYAQHWGPVIRPAAERVLDLATPVIAAGLTAGREPH
                     VLDVGAGTGTLAIRALELWPALQVTAIDPSGGMLELARRHAAERLPMAVRRRLHTEVA
                     PADEMPFEDGAVDVAVSSFVLQLVASRAAAMREVRRVLRPGGLFAWVAWQHTNRVYEP
                     DRVANDVLDDAGFDPPEGEGPSGDIASPAAAAAGMRKAGFRDVTAVTDEMAHAWTPAD
                     YLAFFTQFDEESLFADLEPDERVEIEGKISQGLARLSREQMTLRLPVIYVTGRAP"
     gene            3413..4156
                     /locus_tag="VIF63_02205"
     CDS             3413..4156
                     /locus_tag="VIF63_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEY8238223.1"
                     /translation="MILDVLSGTILNAAAVAIGTTIGLLAATRVSTRLQESLTSGLGM
                     FTLVLAMSMGLQVFTDPQARTQDSLAVLGGLLLGVAIGEWLRLHDRLEDLGAWFQRRL
                     SRGDRPSRVSDAFVTTSIVFCVGPLTILGSLDNGLRGDITLLATKSLLDGVASVAFAA
                     AMGAGVYLSILTVIVVQGGIALGAFLLAGFFDPVTILVLTSLAGITLLGVGLRLLELK
                     QVRLANFLPGLILAPLLVRLFDAVRAAFP"
     gene            4179..5264
                     /locus_tag="VIF63_02210"
     CDS             4179..5264
                     /locus_tag="VIF63_02210"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8238224.1"
                     /translation="MNLPFDPPIEPMLAKPADALPQGEGWLFEPKWDGFRALVFKDGD
                     EVYIQSRDLKPLDRYFPELAAPLRASLPERCVLDGEVVIAGDGGLQFEALLLRIHPAA
                     SRVKMLAEQSPASFVAWDLLALGDEDLRAVPQGERRARLEAAIGASAKPPIHLTPATT
                     DRALAVDWFDRFEGAGLDGVIAKRLDGTYQPGKRAMIKVKHQRTADCAVAGFRWHKNG
                     PNTHVGSLLLGLFDDAGTLHSVGVTSSFTWEKREALTKELAPLREKALEGHPWGEWAE
                     WAQAAEASGQRLPGATSRWNRGKDLSWEPLRVERVAEVAYDHLQGDRFRHGTTFLRWR
                     PDKQPADCRYDQLEETAAYELEKIFRG"
     gene            5361..5717
                     /locus_tag="VIF63_02215"
     CDS             5361..5717
                     /locus_tag="VIF63_02215"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HEY8238225.1"
                     /translation="MDEQTSAPGTTPPPAPGSNWAAPPPPPRRPGFNWADFFAFRYMI
                     TPQIIRVVYVIGVVLVTLAALASVNSFGFGGGGILSALFLLVVGNLYLRVIMELLVVF
                     FGMHESLRSIERQDRR"
     gene            complement(5718..7199)
                     /locus_tag="VIF63_02220"
     CDS             complement(5718..7199)
                     /locus_tag="VIF63_02220"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEY8238226.1"
                     /translation="MPKEQEVLRVGDREVTVTNPGKVFFEAIGATKLDLVRYYLAVAP
                     GALAGAGGRPMALKRFVNGAAGEAFFQKRAPDNRPEWLRTVTLSFPSGRTADEIVVDD
                     AAGLAWIVNLGCIDLNPHPVRADDLDHPDELRVDLDPVPGVGWAQIRDVAMVTKESLE
                     AVGLVGWPKTSGSRGIHINVRIERKWTYPEVRRAALAIARDVERRAPSIATSKWWKEE
                     RHGVFLDYNQNAKDRTVASAYSVRPLPDARVSTPLSWDEVPTAEAEAFTIATVPARFA
                     SIGDPGAGIDGAVGSLEAVLELSQRDEAEGLGDAPWPPNYRKQEGEPPRVQPSKQRRA
                     TADYNTPEAEAEREKNRAAMEKRFTAELERRAAAKAAGEEAPATRPTPTGRRKTNVPV
                     IEISRAKSKAEALEGLERWKVRHPDAAASLDVADVLVDGKRGRSSLWYRVRVNLTHVP
                     EADRPPQEALDPDYDPWAEYAFPDRSGQLERAPRSRKGEVAPD"
     gene            7252..7638
                     /locus_tag="VIF63_02225"
     CDS             7252..7638
                     /locus_tag="VIF63_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8238227.1"
                     /translation="MTEPPPEHAMRYLILIYEQNSANPPAEPPPADVVEATSKAYNAY
                     TQMLKDRGAFLGGDALQPVTTATTVRIRDGQTMTTDGPFAETKEALGGFYMIEAADLD
                     DALALAAACPAAEYGSVEVRPIWEYA"
     gene            7651..8940
                     /locus_tag="VIF63_02230"
     CDS             7651..8940
                     /locus_tag="VIF63_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8238228.1"
                     /translation="MADSTRAHEVVDRLFREESGRAVATLIRVLGDFDLAEEAVQDAF
                     AKALEVWPERGVPDNPAAWITTTARNAAIDRIRRRRRLVDKTVELQREAEVEMSDPGG
                     WTRGGEEAPATDGIDDRLRLIFTCCHPALAPDARVALTLRTLGGLSTPEIAHAFLVPE
                     VTLAQRLVRAKKKIRDAGIPYRVPPAAVLPERLDAVLRVLYLIFNEGYSASAGEALVR
                     RELSGEAIRLGRLLVELMPDEPESLGLLALMLLHDARREARTNSAGELVLLEDQDRDQ
                     WNRAEIDEGQAVLDRALRAGRPGPYQVQAAIAALHDAATTPGATDWAQIAALYGRLAN
                     LTPSPVVELNRAVAIAMAEGPAAGLELLDAIADDERLATYQYFHSARADLLRRLGRRP
                     AAAEAYRRAIELTSNEIERAFLTRRLAEVEGEAARPN"
     gene            9061..>9394
                     /locus_tag="VIF63_02235"
     CDS             9061..>9394
                     /locus_tag="VIF63_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8238229.1"
                     /translation="MRSESLSWAARGAGLAVGVGTVLVLAGLLSAARDVVLLVFIAVL
                     LGAALEPLVSVIRSRTGIGRGLSILVVYAAFLSAVVGIAVFIVPAALVQLGSALGHLP
                     AFLDQIRAW"
BASE COUNT         1304 a         3401 c         3276 g         1413 t
ORIGIN      
        1 ttggcggtcg cggccgcctg atcgaccgcc tgttccaccg aggtccgcag gtcctctcgc
       61 cggaggtcca gcaggacgag gccggaatcg agcttggaca gctccagcag gttctgggcg
      121 agccagtcga gacgctccag ctgggcgcgg ctcgtctcga ggaactcctg gcgcgtcgag
      181 gcgtcgtcgg ccgcgccctc ggtgagcagc tcgttgaacg tgcgcagcgc ggcgatcggc
      241 gtgcgcagct cgtgcgagac gtcggcgagg aactcgcgcc cgcggtcgcg gtcgctgcgg
      301 atgatcccga tgctctcctg gaggcgctcg gccatcgtgt tgaactggag cccgagctcg
      361 gacagctcga tcggcccgtc ctcgatcgcc tcggacggca cgcgccgggc gaagtcgccc
      421 tcggcgaggt cacgagtcgc ctcggtcagg cgccgcaggg gcgtggtgat gcgccgcgtg
      481 agcatcgccg ccaccaccac tgcgaccgcc agcgcgatga ggccggtgac cgcgagcagg
      541 ccggagacgt tctggatcgc cgcggcgcgg taggtgaagg ggtcctgcaa aacgacctcg
      601 acgccgtagg gcaatgacgc cgaggtgacc ttctcgagga ccggcttgct ggacatgcgc
      661 tcctggacct ggttcggctt cggtgacgcg agctcctcgg cgaagaacgt gccgttcgcg
      721 gccggctgga acgtgccgtt cgcgaggccg agggtcaccc gcacgtccgc ctgggcgagc
      781 tggtcggcaa tgatccgttg ctgcccgctc cgctggagct ccgtcacgat ttccggatcg
      841 acgagcccgt ctgcggagac gaccggccgg cccgcccgga cgttgctcgt gccttcggcg
      901 atgacgatca cgtactgggc gacgcttcgc gcccgcgagg cgaggtcgtc ctcctgctgc
      961 tggtcgaaat acgcgccgag ccggttgacc accagcagcc cgaccagccc gagggtcacc
     1021 gcgacgacgg cgacgaacgc cagcgtcagg cggccctgga acgtcctcgg agcgagcccg
     1081 gcggcctggc cgtcagcgct cggcgaatgc gtacccgaca ccgcggatgg tctggacgaa
     1141 ggccgggttc gtggggtcgt cctcgagctt gtcgcgcagg tggctcacgt ggacgttgat
     1201 cgtcttctcg tcctggtcga gcgcctcgta gtcgcgcagc aggtcgagca gctctgcccg
     1261 ggtgtagacg cggccgggct tgctggcgag gtggcgcagg atctcgaact cggtcggggt
     1321 gaggctgatc cgccggtcgc cccgctggac ccggcggcga tcgaggtcga tgagcaggtc
     1381 cctgtgccgg atcacccgtc cgccgacggc gtcggcgagg tcgccgtagg cacggcgcag
     1441 gagcgccttc acgcgactca gcagctcccg gagcccgaac ggcttggtca ggtagtcgtc
     1501 cgcgccgagc tcgaggccga tgaccttgtc gacctcctca tcgcgggcgg tcagcatcag
     1561 gaccggcgac ttcgtcccgg cggcgcggag gcggcggagg acctcgaagc cgtccatgcc
     1621 cggcaggcgc acgtcgagca ggaccaggtc cggcgccgag ttcgtcgcga gctcgaggcc
     1681 ctcctcgccg gagcgggcga gcgtgacctc atagccttcc tgctccaggg cgtactggag
     1741 cccacgcgca acggcgtgct catcctcgac gaccaggatc gtggctgcca ttggaccttc
     1801 atggtagggt taccagcccg atcgaccctt ccggaggctc acttccgcat gtccgccgct
     1861 cctgcccgcc cgaagctcgc cgccgccagc cgtacgctga ccggcaagaa ggtctcccag
     1921 atgcgcgccg ccggccgcct gcccgcggtc gtcttcggcc gcggcatcga ctccgatagc
     1981 gtgtcggtcg acacccatga gttcgagcag cttcggcgcc acgcgggctc caacaccctg
     2041 atcgacctgt cggtcgacgg cgcggccccc gcgccggtgc tggtctacgg cgtccagacg
     2101 catcgggtca cccgccggcc gctgcacgtc gatctgtacg tcgtgcgcat gaccgaggag
     2161 ctgacggtcg acgtgccgct cgtgtccgag ggcctgtccg acgcggtcga gaacgccgcc
     2221 ggcaccctgc tccacgtgat cgaccacgtg cgcgtccgcg cgctgcccga ccacctgccc
     2281 gagtcgatcc actactcgat cgagtcgctg cgcaccttcg acgaccagat ccacgtccgc
     2341 gatctcgaga tcccggccga cgcgacgctc atcaccgacg gcgacgaagt ggtcgccaag
     2401 gtcctgccgc cgcgcgtcga ggaggagccg gtcgtcgccg aggccgtcga gggcgccgag
     2461 ggcgaggaag gtgccgaggc tgccgagggc gccgagggtg ccgagtcgag cgagggcgac
     2521 gagggctgat cccactgcgg cgcttgccgc agctacggag cgcgacccgt cacgtagatc
     2581 accggcaggc gcagcgtcat ctgctcgcgg ctgagccgcg ccaggccctg gctgatcttg
     2641 ccctcgatct cgacccgctc atccggctcg aggtccgcga agagcgattc ctcgtcgaat
     2701 tgcgtgaaga acgcgaggta gtcggccggc gtccaggcgt gggccatctc gtcggtcacg
     2761 gcggtgacgt cgcggaagcc ggccttgcgc atgccggcgg cggccgcggc cggcgacgcg
     2821 atgtctcccg acggcccctc gccttcgggc gggtcgaagc cggcatcgtc gaggacgtca
     2881 ttcgccacgc ggtccggctc gtagacccga ttcgtgtgct gccaggcgac ccacgcgaag
     2941 aggccgccgg ggcggagcac gcggcgaacc tcgcgcatcg ccgcggcgcg gctcgcgacc
     3001 agctggagca cgaacgacga gaccgcgacg tccacggcgc cgtcctcgaa cggcatctcg
     3061 tctgccggcg cgacctcggt gtggaggcgg cggcgaacgg ccatcgggag gcgctcggcg
     3121 gcatgccgac gcgcgagctc gagcatcccg ccggacggat cgatggcggt gacctggagc
     3181 gcgggccaca gctcgagggc gcggatggcg agcgtcccgg tccccgcacc gacatcgagc
     3241 acgtgcggct cgcgcccggc cgtcagaccc gccgcgatca ccggcgtcgc gagatcgagc
     3301 acgcgctcgg ccgcgggccg gatgaccgga ccccaatgct gggcgtagcc ctcggcaatc
     3361 tcgtcgtagc tcacctaccg atcgtagcgg cgcccgacct accattcggg cggtgatcct
     3421 cgacgtcctg tccggcacga tcctcaacgc ggcagcggtc gcgatcggga cgaccatcgg
     3481 gctcctcgct gcgacgcgcg tctccacgag gctccaggag agcctgacca gcggcctggg
     3541 catgttcacg ctcgtcctgg ccatgtcgat gggcctccag gtcttcaccg acccacaggc
     3601 ccggacccag gattcgctcg ccgtcctcgg tggcctgctg ctcggggtgg cgatcggcga
     3661 gtggctgcgc ctgcacgatc ggctggagga cctcggcgcg tggttccagc gacggctcag
     3721 ccggggcgac cgtccgtcgc gggtctcgga cgcgttcgtc acgacgagca tcgtgttctg
     3781 cgtcggcccg ctcacgatcc tcggcagcct cgacaacggc ctccgcggcg acatcaccct
     3841 gctggccacg aaatccctgc tcgatggcgt cgcgagcgtc gcgttcgcgg cggccatggg
     3901 cgcgggcgtc tacctctcga tcctgaccgt catcgtcgtc cagggcggga tcgcgcttgg
     3961 cgccttcctg cttgccgggt tcttcgaccc cgtgacgatc ctggtgctga cctcgctcgc
     4021 cgggatcacc ctgctcggcg tcgggctgcg actgctcgag ctgaagcagg tccggctcgc
     4081 caacttcctg ccggggttga tcttggcacc gctgctcgtc cgcctgttcg acgccgtgcg
     4141 cgcggcgttc ccgtagcagc gcgggtaccg ttgacggcgt gaacctcccc ttcgacccac
     4201 cgatcgagcc gatgctcgcg aagcccgcgg acgccctgcc ccagggcgaa ggctggctgt
     4261 tcgagccgaa gtgggacggc tttcgggcac tcgtcttcaa ggacggcgac gaggtctaca
     4321 tccagtcgcg tgacctgaag ccgctcgatc gctacttccc ggagctggcg gcgccgctgc
     4381 gagcctcgct cccggagcgc tgcgtgctcg acggcgaggt cgtcatcgcg ggcgacggag
     4441 gcctccagtt cgaagccctg ctgctgcgca tccaccctgc cgcctcgcgg gtgaagatgc
     4501 tggccgagca gtcgccggcg tcgttcgtgg cgtgggacct gcttgcgctt ggcgacgagg
     4561 acctccgggc cgtgccgcag ggcgagcgac gcgcccgcct cgaagccgca atcggcgcca
     4621 gcgccaagcc gccgatccac ctcaccccgg ccacgaccga tcgcgcactg gcggtggact
     4681 ggttcgacag gttcgagggc gcgggcctgg acggcgtgat cgccaagcgc ctcgacggca
     4741 cgtaccagcc cggcaagcgg gcgatgatca aggtcaagca ccagcgcacc gccgactgcg
     4801 cggtcgccgg cttccgctgg cacaagaacg ggccgaacac gcacgtcgga tcgctgctgc
     4861 tcgggctgtt cgacgatgcc ggcacgctgc acagcgtcgg cgtgacctcc tcattcacgt
     4921 gggagaagcg ggaagccctg acgaaggagc tcgcgccgct gcgcgagaag gcgctcgagg
     4981 gccatccctg gggcgagtgg gccgagtggg cccaggccgc cgaggcgtcg ggccagcgcc
     5041 tgcccggcgc aacctcacgc tggaaccggg ggaaggacct gtcctgggag ccgcttcgcg
     5101 tcgagcgggt cgccgaggtc gcctacgacc acctccaggg cgatcgcttc cgccacggca
     5161 cgacgttcct gcgctggcgc ccggacaagc agccggccga ctgccgctac gaccagctcg
     5221 aggagaccgc ggcctacgag ctcgagaaga tcttccgcgg ctgacgcgtc agcaggcggc
     5281 acgcaggcgc tgccccgggt ctagggttag ccggacaggc gtccgcgccc cgcggtcgcc
     5341 atcgatcgcg gagggacgac atggacgagc agacctcggc accaggcacg acaccgccgc
     5401 cggctcccgg ctcgaactgg gccgcgccgc caccgccgcc aaggcggccg ggcttcaact
     5461 gggcggactt cttcgccttc cgctacatga tcacgccaca gatcatccgg gtcgtgtacg
     5521 tgatcggcgt cgtcctggtc acattggcgg cacttgccag cgtcaacagc ttcgggttcg
     5581 gcggcggggg catcctcagc gccctcttcc tgctcgtcgt cggcaacctg tacctgcgcg
     5641 tgatcatgga gctgctcgtc gtgttcttcg ggatgcacga atcgctgcgc tcgatcgaac
     5701 gccaggaccg ccgctagtca gtcgggcgcg acctcgcctt tccgggagcg gggcgcacgt
     5761 tccagctggc cggaacggtc cgggaaggcg tattcggccc acgggtcgta gtcggggtcg
     5821 agcgcttcct ggggtgggcg gtcggcttcc ggcacgtggg tgaggttcac gcggacccga
     5881 taccagagcg atgagcggcc gcgcttgccg tcgacgagga cgtcggcgac atcgaggctc
     5941 gccgcggcgt cgggatggcg aaccttccag cgctccaggc cctcgagcgc ctcggccttc
     6001 gacttggccc gcgagatctc gatgaccggc acgttcgtct tgcggcggcc ggtgggcgtc
     6061 gggcgggtgg ccggcgcctc ctcgccggcg gccttcgcgg cggcgcggcg ctccagctcc
     6121 gccgtgaagc gcttctccat cgccgcgcgg ttcttctccc gctccgcctc ggcctcgggg
     6181 gtgttgtagt ccgccgtcgc ccggcgctgc ttcgacggct ggacgcgcgg cggctcgccc
     6241 tcctgcttgc ggtagttcgg cggccacggc gcgtcgccca agccctcggc ttcgtcgcgc
     6301 tgcgacagct cgaggaccgc ttcgagcgag ccaacggcgc cgtcgatgcc cgcgcccgga
     6361 tcgccgatcg acgcgaaccg cgccggcacg gtggcgatcg tgaacgcctc ggcctcggca
     6421 gtcgggacct cgtcccacga caagggcgtc gatacccgcg cgtccggcag cggccgcacc
     6481 gagtacgccg aggcgaccgt gcggtccttc gcgttctggt tgtagtcgag gaagacgccg
     6541 tggcgctctt ccttccacca cttcgacgtg gcaatcgatg gcgcccgccg ctcgacatcg
     6601 cgcgcaatcg ccagcgccgc ccgccgtact tcggggtagg tccatttccg ttcgatccgc
     6661 acgttgatgt gaatccctcg tgagcccgac gtcttcggcc agcccacgag cccgaccgcc
     6721 tcgagcgatt ccttcgtgac catcgcgacg tcccggatct gcgcccagcc gacacccggg
     6781 accgggtcga ggtcgacacg cagctcgtcc gggtggtcga ggtcgtccgc ccgaaccggg
     6841 tgcgggttca ggtcgatgca gccgaggttg acgatccagg caaggcccgc cgcgtcgtcg
     6901 acgacgatct cgtcggcggt ccggcccgac gggaacgaca acgtaacggt tcgcagccac
     6961 tccggccggt tgtccggcgc gcgcttctgg aagaaggcct cgccggcggc gccgttcacg
     7021 aaccgcttga gggccatcgg ccggccgccg gcgccggcga gggcgcccgg agccacggcg
     7081 aggtagtagc ggacgaggtc aagcttcgtt gccccgatcg cctcgaagaa gaccttgccc
     7141 gggttcgtga cggtgacctc acggtcgccg acccggagga cctcctgctc cttcggcatg
     7201 cggtcgatgc tagcgtgctg tcgatttccg acggccccgt tcgtcgatcc gatgaccgaa
     7261 cccccaccgg agcacgccat gcgctacctg atcctgatct acgagcagaa ttcggccaac
     7321 ccgcccgccg agccgccgcc cgcggacgtc gtcgaggcga cgagcaaggc ctacaacgcc
     7381 tacacgcaga tgctcaagga ccgcggcgcg ttcctcggtg gcgacgccct ccagccggtc
     7441 acgaccgcga cgaccgtgcg catccgtgac ggccagacaa tgaccaccga tgggccgttc
     7501 gccgagacca aggaagccct cggcggcttc tacatgatcg aggcggccga tctcgacgat
     7561 gcgttggcgc tcgcggcggc atgcccggct gcggagtacg gctccgtcga ggtccgcccg
     7621 atctgggaat acgcgtaggc acaggcgcga ttggccgata gcacccgcgc ccacgaggtc
     7681 gtcgaccgcc tgttccgcga ggagtcaggt cgggccgtcg cgaccctcat ccgagtgctc
     7741 ggtgacttcg acctcgccga ggaggcggtc caggatgcgt tcgcgaaggc actcgaggtg
     7801 tggccggagc gcggggtgcc cgacaaccct gcagcgtgga tcaccacgac cgcgcgcaac
     7861 gccgccatcg accgaatccg gcggcggcgg cgcctcgtcg acaagaccgt cgagctccag
     7921 cgcgaggccg aggtcgagat gagcgatccg ggcggctgga cccgcggcgg cgaggaggcc
     7981 cccgcgacgg acggcatcga cgaccgcctg cggctgatct tcacgtgctg ccacccggcg
     8041 ctcgcccctg atgcgcgggt cgcgctgacc ctgcggaccc tcggcggcct gagcacgccg
     8101 gagatcgccc acgccttcct cgtgcccgag gtgacgctcg cgcagcgcct tgttcgcgcc
     8161 aagaagaaga tccgcgacgc cggcattccc taccgcgtcc cgccggccgc ggtgctgccg
     8221 gaacgactcg atgcggtcct gcgcgtgctc tacctcatct tcaacgaggg ctacagcgcg
     8281 agcgccggcg aggcgctggt acgccgtgag ctgtccgggg aggcgatccg cctcggccgc
     8341 ctcctcgtcg agctcatgcc cgacgagccc gagagcctcg gactcctggc gctcatgctc
     8401 ctgcacgacg ctcggcgcga ggcgcgcacg aactcggccg gcgaactcgt cctgctcgag
     8461 gaccaggatc gcgaccagtg gaaccgcgcc gagatcgacg agggccaggc cgttctcgac
     8521 cgggcgctgc gcgcgggccg gcccgggccg taccaggtcc aggcagcgat cgcggcactc
     8581 cacgatgccg ccaccacacc cggcgcgacc gactgggcac agatcgcggc gctgtacggg
     8641 cgccttgcga atctcacccc ctcgcccgtc gtcgagctga atcgcgccgt cgccatcgcg
     8701 atggctgagg gtcccgccgc cggcctcgag ctgctcgacg ccattgccga cgatgagcgc
     8761 ctcgccacct accagtactt ccactcggcg cgcgccgacc tgctccgccg gcttggacgg
     8821 cgccccgcag cagccgaggc gtatcgccgc gcgatcgagc tgacgtcgaa cgagatcgag
     8881 cgcgcgttcc tcactcgacg gctggccgag gtcgaaggcg aggcggcgcg gccgaactga
     8941 cgatcgcgcc gccacgcgaa gcgcgaggcg cgaggcaatt gcaaggcgcg cggccaccca
     9001 cggcgttcgg cgtggcccac cggtccctca cgatccccac cgcgaggcag ggagggatgg
     9061 atgcgctcgg aatcactcag ctgggcggca cggggagcgg ggctcgccgt cggggtcggg
     9121 accgtgctcg tgctcgccgg gctcctgtct gcggcgcgcg atgtcgtcct gctggtcttc
     9181 atcgccgtgc tgctcggggc ggcgctcgag ccgctcgtgt ccgtgatccg gtctcggacc
     9241 gggatcgggc gtggcctgtc gatcctcgtc gtctacgcag cgttcctctc tgcggtcgtc
     9301 ggtatcgcgg tattcatcgt ccccgccgcg ctcgtgcagc tcgggtccgc gctcggccac
     9361 ctgcccgcct tcctcgacca gatccgggcg tgga
//
LOCUS       DATBSW010000083         4968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_817266, whole genome shotgun sequence.
ACCESSION   DATBSW010000083 DATBSW010000000
VERSION     DATBSW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817266"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VIF63_02240"
     CDS             <1..595
                     /locus_tag="VIF63_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238230.1"
                     /translation="WMDLADERLAGKNIRCIVSPANDDIFEIDPIIAAAKLIELGESN
                     LIDLDGISLVSTGWANPTPWNTFRELPEPELRERIDGLVKDVPDRRRAIFNFHAPPFG
                     SNLDNAPKIGADMRYVSGGQALIPVGSHAIRDAILEYGPVLSLHGHIHEGRGAVKLGK
                     TLSVNPGSTYEDGVLQAAIVDLDVKKAEVKRYLLING"
     gene            642..2423
                     /locus_tag="VIF63_02245"
     CDS             642..2423
                     /locus_tag="VIF63_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8238231.1"
                     /translation="MAKAPALFLRNATGLVKGWSGFDAFAYAFMSVNLVALGMFYSLA
                     IFGYVPDGSAVVSILITAVGMTFMAIAYACLIAAMPRAGGDYVWQTRILDGIPGAVVG
                     GAFGAVALYLVGTALALDGTVTLGATALGVIAGATLGIKRGGIGFVLSATGWWFILAL
                     WAPIYGFILKIEFIQPMAALLGWKEGVDFFGTADGTFVVSILTIVVTSALVALGMAGY
                     ARIQRWCLYIGLAALAVIFVLMIVSSTDQFIAAFNREGVALFGVENAYQATIDQGTAQ
                     GVNGGDINNFSFGAGTFALVPFMLFFLLYPNWGSTLYGEVRGAGDFRKVLNGMLGGLW
                     ATVGLAIVFVFLAAKTFGWEFFNASNGTYVNYAYGYTPDDPPVIAIWGFPGLLASYLI
                     DSHIIQAIIVFAFGAWWLGWTGTLFLSSTRMIFAAAFDRVLPEWAGRVSEGRGVPWAA
                     LVLIMVPSVVLSYLYAYQAATFYPLTLDATLVIAVTFFGTSIAATILPYWKPDIYNSS
                     AVARYKIVGLPLITIAGALSTLFLGWVLYTWLSDPLYAIGVSNTDSIIFLGVLYGAAA
                     LLYVVARMWRRMQGVDLDAIHAQIPAE"
     gene            2628..4121
                     /locus_tag="VIF63_02250"
     CDS             2628..4121
                     /locus_tag="VIF63_02250"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8238232.1"
                     /translation="MPSKPAVHPTATPTAVAAAPTLFIDGRWQAAASGATFEATSPAT
                     GQVIGQISEGGRADATLAIEGAHRAAPAWGRATAFERAAALERIAASIEARKDALARA
                     LTEDQGKPLQAEAYGEVEELILYFRMAAADATRIEGLIPPSVDPMKRVLVYRVPKGVV
                     GVITPWNWPYTMPAELIAPALAAGNAVVWNPARLTSHCSVALADAIVEANLPPGVFNL
                     VTGPGDEVGDEIAGDPRVAAIGFVGSTKTGQKIAHRAAGKELLLELGGNGPLVILDDA
                     DLEAAVRATITACFLNAGQSCTAGERVLVDRAIHDQYLELLVETVGREVRLGDPLDGA
                     TTMGPLNNEKTAAKAEHQVAEAIERGATLHAGGRRASHLGSNLFFQPTILDRVTESME
                     IAREETFGPVVPITAIDGEAEALEIIGSSPYGLLTAVFTRDLARGLRFAESARAGWVN
                     INEGTNYWESHLPFGGRAGSQSGLGRVGGRFAIERLTELKTIVVNLA"
     gene            4148..>4968
                     /locus_tag="VIF63_02255"
     CDS             4148..>4968
                     /locus_tag="VIF63_02255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8238233.1"
                     /translation="MAGLPAKAKVVVIGAGIVGNSMAWHLARLGWRDIVLLEKGTLPS
                     PGGSTGHASNFIFLTDHSKEMTAFTLDSVQQYEELGVFTMTGGIEVARTPERMQELTR
                     RITSSKSWGIEPAELISTKRVKELVPFINEDIVLGGVYWPKVGVVDSLEAGTLMRKKA
                     QELGALTIGAGVEVTGLETKDGRITRVRTDHGDIETDTVVIACGVWSPRIAAMAGASI
                     PLTPAVHQMIDIGPVAIFENVPVAVGYPIIRDVDTNMYERQHGNEFEVGSYAHRT"
BASE COUNT          769 a         1716 c         1656 g          827 t
ORIGIN      
        1 ctggatggac ctcgctgacg agcgcctcgc cggcaagaac atccggtgca tcgtctcgcc
       61 ggcgaacgac gacatcttcg agatcgatcc gatcatcgcc gcggcgaagc tgatcgagct
      121 cggcgagagc aatctcatcg acctcgatgg catctcgctc gtctcgaccg gctgggccaa
      181 cccgacgccg tggaacacgt tccgcgagct tccggagccc gagctccgcg agcggatcga
      241 cggcctggtg aaggacgtcc cggatcggcg ccgggcgatc ttcaacttcc acgcgccgcc
      301 gttcggttcg aatctggaca acgccccgaa aatcggcgct gacatgcgct acgtgtccgg
      361 cgggcaggcc ctgatcccgg tcgggtcgca cgcgattcgg gacgccatcc tcgaatacgg
      421 tccggtcctg tcgttgcacg gccacatcca cgaaggacgc ggcgccgtga agctcggcaa
      481 gaccctttct gtgaaccccg gctcgaccta cgaggacggt gtcctccagg ccgcgatcgt
      541 cgatctcgac gtcaagaagg cggaggtgaa gcgctatttg ctgatcaacg gctgaccagg
      601 tcacgagtag ggagggaagg agctcgatga ctacaggaga catggccaaa gccccggcgc
      661 tgttcctgcg gaacgcgacc ggtctcgtca agggctggtc cggcttcgac gcgtttgcct
      721 acgcgttcat gtccgtgaac ctcgtggccc tcggcatgtt ctatagcctc gcgatcttcg
      781 gctatgtgcc cgacggatcg gcagtcgtgt cgatcctgat caccgcggtc ggcatgacct
      841 tcatggccat cgcctatgcg tgcctcatcg ccgcgatgcc ccgcgccggc ggcgactacg
      901 tctggcagac gcgaatcctt gacggcatcc ccggcgcggt tgtcggcggt gcgttcggcg
      961 cggtggcgct gtacctcgtc ggaacggcgt tggcacttga cgggaccgtg acgctcggcg
     1021 cgacggcact cggtgtcatc gccggcgcca cgctcggaat caagcgcggt ggcatcggct
     1081 tcgtcctctc ggcgacgggc tggtggttca tcctcgccct ctgggcgccg atctacgggt
     1141 tcatcctgaa gatcgagttc atccagccga tggcggccct gctcggctgg aaggaaggcg
     1201 tcgacttctt cgggacggcg gacggcacat tcgtcgtctc gatcctgacg atcgtcgtga
     1261 cgtcggccct cgtcgccctc ggcatggccg ggtacgcccg aatccagcgc tggtgcctgt
     1321 acatcggcct cgccgcgctc gcagtgatct tcgtcctgat gatcgtgtcc agcacggacc
     1381 agttcatcgc cgccttcaac cgcgaagggg tggcgctctt cggtgtcgag aacgcctacc
     1441 aggcgaccat cgaccagggc accgcccagg gcgtgaacgg cggggacatc aacaacttct
     1501 cgttcggggc gggcaccttc gcgctcgtgc cgttcatgct gttcttcctg ctgtacccga
     1561 actggggctc cacgctgtac ggcgaggtcc gcggtgcggg tgacttccgc aaggtcctga
     1621 acggcatgct cggcgggctg tgggccacgg tcggcctcgc gatcgtgttc gtgttccttg
     1681 ccgcgaagac gttcggctgg gagttcttca acgcgtcgaa cgggacatac gtgaactatg
     1741 cgtacggcta cacgccggat gaccccccgg tcatcgcgat ctggggcttc ccgggcctgc
     1801 ttgcctcgta cctgatcgac agccacatca tccaggcgat catcgtgttc gccttcggtg
     1861 catggtggct cggctggacg ggcacgctgt tcctgtcgtc gactcgaatg atcttcgcgg
     1921 cggccttcga tcgcgtcctg cccgagtggg ccggccgtgt ctccgagggc cgcggggtgc
     1981 cctgggcagc cctcgtgctg atcatggtcc cgtcggtcgt cctgagctac ctgtacgcgt
     2041 accaggcggc gaccttctat ccgctgacgc tcgatgcgac gctcgtcatc gcggtgacgt
     2101 tcttcggcac ctcgatcgcg gcgacgatcc tgccctactg gaaacccgac atctacaact
     2161 cgtcggcggt cgcgcgatac aagatcgtcg gcctgccgct gatcacgatc gccggtgcgc
     2221 tctcgacgtt gttcctgggc tgggtgctgt acacgtggct cagcgatccg ctctacgcga
     2281 tcggcgtgag caacaccgac tcgatcatct tcctcggcgt gctctacggc gcggccgcgc
     2341 tcctgtacgt cgtcgcccgc atgtggcgcc ggatgcaggg cgtcgacctc gacgcgatcc
     2401 acgcgcagat ccccgcggag taacaacccg cactccgtgc cggcctttgc cggcaggtca
     2461 atcggttgca atcgaagggc gcccaccggg cgcccttcgc gattccaggg cgtgcgagct
     2521 ccatgagttg cgtcgcgatg ggtcggcagg gcagactgtc cgcgcggcgg atacgtccgg
     2581 cccgcctggg gtgcagcaag cccacgtagt tccgtccagg agctgttgtg ccctccaagc
     2641 ctgcggtcca cccgaccgcc acacccaccg ccgtggcggc tgcgcccacc ctgttcatcg
     2701 atggccgctg gcaagccgcc gcgagcggcg cgaccttcga agcgacgagc ccggcgacgg
     2761 gccaggtgat cggccagatc tccgaaggcg ggcgggccga cgccaccctc gctatcgaag
     2821 gggcacaccg cgccgcgccc gcctggggtc gcgcgaccgc gttcgagcgg gcggcggcgc
     2881 tggagcgcat cgccgcgtcc atcgaagcgc gcaaggacgc gctcgcccgc gccctcaccg
     2941 aggaccaggg caagccgctc caggccgagg cctacggcga ggtcgaggag ctgatcctct
     3001 acttccggat ggccgccgcc gacgcgaccc ggatcgaagg cctgatcccg ccctcggtcg
     3061 atccgatgaa gcgcgtgctc gtctaccgcg tcccgaaggg cgtcgtcggc gtgatcacgc
     3121 cgtggaactg gccgtacacg atgcccgccg agctcatcgc accggccctc gctgccggca
     3181 acgcggtcgt ctggaacccg gcccggctga cctcgcactg ctctgtcgcg ctcgcggatg
     3241 ccatcgtcga agcgaacctc ccgccgggcg tgttcaacct cgtcacaggg ccgggcgacg
     3301 aggtcggcga cgagatcgcc ggcgacccgc gggtggccgc gatcggcttc gtcggctcga
     3361 cgaagacggg ccagaagatt gcccatcgcg cggccggcaa ggagctgctc ctggagctcg
     3421 gcgggaacgg gccgctggtg atcctcgacg atgccgacct cgaggccgcg gtccgcgcga
     3481 cgatcaccgc ctgcttcctc aacgcgggcc agagctgcac tgccggcgag cgcgtcctcg
     3541 tcgaccgcgc gatccacgac cagtacctcg agctgctcgt ggaaacggtc gggcgcgagg
     3601 tgcggctcgg cgatcccctc gatggcgcga cgacgatggg cccgctcaac aacgagaaga
     3661 cggccgcgaa ggccgagcac caggtcgccg aggcgatcga gcgcggcgcg acgctccacg
     3721 ccggcggccg ccgcgcgtcc cacctcggca gcaacctgtt cttccagccg accatcctcg
     3781 accgcgtgac cgagtcgatg gagatcgcgc gcgaggagac cttcgggccg gtcgtgccga
     3841 tcaccgccat cgacggcgag gccgaggcgc tcgagatcat cggcagctcg ccgtacgggc
     3901 tcctgacggc cgtgttcacg cgcgacctcg cccgcggcct gcggttcgcc gagtcggctc
     3961 gggccggatg ggtcaacatc aacgaaggca ccaactactg ggagtcgcac ctgcccttcg
     4021 ggggccgggc cggctcgcag agcggtctcg ggcgcgtggg tggccggttc gccatcgagc
     4081 gcctgacgga gctcaagacg atcgtcgtga atctcgcctg actggcgaac gcttctcgga
     4141 ggaaaacatg gccgggctgc cagcaaaggc gaaggtcgtc gtcatcggtg ccgggatcgt
     4201 cgggaacagc atggcctggc acctcgcgcg cctgggctgg cgcgacatcg tgctgctcga
     4261 gaaaggcacc ctgccgtcgc ccggcggctc gaccggccac gcctccaact tcatcttcct
     4321 caccgaccac tcgaaggaga tgaccgcgtt caccctcgat tccgtgcagc aatacgagga
     4381 gcttggcgtc ttcacgatga ccggcggcat cgaggtcgcg cgcacgccgg agcggatgca
     4441 ggagctgacg cggcgcatca cctcgtcgaa gtcgtggggc atcgagccgg ccgagctgat
     4501 ctcgacgaag cgcgtgaagg agctcgtgcc gttcatcaac gaggacatcg tcctcggcgg
     4561 cgtgtactgg cccaaggtcg gcgtcgtcga ctcgctcgag gccggcacgc tcatgcgcaa
     4621 gaaggcccag gagctgggcg cgctgacgat cggtgcgggg gtcgaggtca ccgggctcga
     4681 aacgaaggat ggccggatca cgcgcgtacg gacggaccac ggcgacatcg agaccgacac
     4741 ggtggtcatt gcctgcggtg tctggagccc gcgcatcgca gcgatggccg gcgcctcgat
     4801 cccgctcacg ccggcggtcc accagatgat cgacatcggg ccggtggcga tcttcgagaa
     4861 tgtcccggtc gccgtcggct atccgatcat ccgcgacgtc gacacgaaca tgtacgagcg
     4921 gcagcacggc aacgagttcg aggtcggctc gtacgcccac cggacgat
//
LOCUS       DATBSW010000084         8473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_641031, whole genome shotgun sequence.
ACCESSION   DATBSW010000084 DATBSW010000000
VERSION     DATBSW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641031"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VIF63_02260"
     CDS             <1..915
                     /locus_tag="VIF63_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8238234.1"
                     /translation="GYNGLKAILGNLYGLDIKYYVEVDFEGFRKVVDALGGVTVNVQV
                     PVLDNNFPQPNSHRLRLFVPAGVQHMTGSQALAYARSRKSTSDFERGARQQRLIVSLR
                     QQLDIGQVLRNIDPLSAAVGKSLKTDIPRDLVPQLLGLADRIDTRSIRSVIFTPPFYQ
                     QETLSGPRGYIIQPRLARIRAAIAEAFSIDPGFAEARDALLEEGAELWVLNGSGRNGE
                     AARLSEYLSYLGLAASAPNQKPDTTGLSKTTIRVYNGAEERFPLTLAALQKVFGVTVT
                     PVTDPAVRVDFIVITADSTPELTAPPAP"
     gene            complement(1034..2020)
                     /locus_tag="VIF63_02265"
     CDS             complement(1034..2020)
                     /locus_tag="VIF63_02265"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY8238235.1"
                     /translation="MLTEKSKSETSARPAAAHGPASTDGLIAYFEGNYVPLRDANVNI
                     MTHAFMYGTAVFEGIRAYWNEEQEVLYGLKLREHMERIRRNAGMLLMTDLPPVDEFVR
                     IVVETIRRNEFREDAYIRPCLYKSGKSVGVRLHNLPHDLVVLAVPFGNYIDVDKGVRV
                     MTSTWRRNADDALPARGKIVGGYVNMAFQKSEAELNGFDEALVLTPDGHASEASAANL
                     FVVRDGILLTPPVNDDILEGVTRRAVLELADALGIPHESRSIDRSEIYVADETFLCGT
                     GVQLSPVIELDHRPIGGGAIGPITKQLHEAYFAAVRGRDQRFMHWLTAIPSR"
     gene            complement(2181..3230)
                     /locus_tag="VIF63_02270"
     CDS             complement(2181..3230)
                     /locus_tag="VIF63_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238236.1"
                     /translation="MDLLILAASFVIILAGAELFTNGIEWFGRKLGLAEGAVGSVLAA
                     VGTALPETMIPIVAILFSAEAHSDEIGVGAILGAPFMLATLAMFVTGVAVIWQARHRE
                     TGDVLQVDTKVLSTDMRTFVIAYGLAIAAAIFLPSEPTWPKIVVAIALLLVYAWYVKA
                     HFEADPTVDVEDLAPLRFHRFDSGRTTGSDNAPRLRAVNFQVIVALGLIIVGAIAFVD
                     AVGHVATALGVSQVLLALVVAPIATELPEKFNSIIWIRQGKDTLAMGNITGAMVFQSA
                     IPVSVALIFAPEKWVAAEGSYLAFASAGIAFLASAFIFLPMYRAGRLRGRGLLVGGLF
                     YIVYLGIVVVGISQG"
     gene            complement(3236..3616)
                     /locus_tag="VIF63_02275"
     CDS             complement(3236..3616)
                     /locus_tag="VIF63_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEY8238237.1"
                     /translation="MSGRAEDAVGTGPGSLEVALAHVLQLGTYVSIGLVGLGTLLLLA
                     GGTSPTAGGPPLSLPTLLADLAALRPAGFLWIGIVGVLSTPALRVLRALIGFWRRGER
                     TMALVSLAILLVVAVGVFFGLLTG"
     gene            complement(3613..4443)
                     /locus_tag="VIF63_02280"
     CDS             complement(3613..4443)
                     /locus_tag="VIF63_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8238238.1"
                     /translation="MLVGGVAAGVFGSLLGLGGGLLIVPLLTIGFGLPLREAVGVSLV
                     CVIVTSSASAGVYLQRRQANLRLGMVLELFTGIGAIIGGAIAFLVDERLLAGLFAILL
                     VYVATTILRSARRPATPITADEGAPAAGDVPDEDAATVDPDAATLGLAPAALGRMPFA
                     AVASLGAGVASALLGIGGGLIKVPLMHLGLGVPLRIATATSNLMVGITASTSAFVYLL
                     RGGIDAYATGPTAIGVFVGASLGSRLGHRLDVRVLRLLFVVVLLFTAFEMGRRALVVE
                     "
     gene            complement(4537..4812)
                     /gene="rpsR"
                     /locus_tag="VIF63_02285"
     CDS             complement(4537..4812)
                     /gene="rpsR"
                     /locus_tag="VIF63_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY8238239.1"
                     /translation="MPPAQARSRTKRDDFYRDRRRRKVCAFCADKTVQIDYKEVNRLR
                     RYLSERAKIEPRRKTGTCAGHQRELSVALKRARHVALLPYSPEHLRP"
     gene            complement(4852..5319)
                     /gene="ssb"
                     /locus_tag="VIF63_02290"
     CDS             complement(4852..5319)
                     /gene="ssb"
                     /locus_tag="VIF63_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8238240.1"
                     /translation="MALSKIQIIGNLGRDPELRYTPNGRPVASFTVAVNQSTKNQQSG
                     EWIESTDWFRVSIWGDRGERAAENLRKGSRVFVDGRFRTREYEANDGQKRMSLDITAE
                     TVLGLDKREAGPEGTFAGAPAGAGAGAPAGAGAPAGGGRSGGYDDTEIDELPF"
     gene            complement(5332..5760)
                     /gene="rpsF"
                     /locus_tag="VIF63_02295"
     CDS             complement(5332..5760)
                     /gene="rpsF"
                     /locus_tag="VIF63_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8238241.1"
                     /translation="MRRYELMLVFRPDAPDDRISAIIDRTTRQITTDGGQIVKVAPWG
                     RRRLAYAIDRHREGAYHIVVFEAPTTVIAEVERALLITEELLRHLIVRQDRPLRASRD
                     SGLDDADETLLPSAEDEEEEDDGRERIDESESEAAPAAID"
     gene            5930..7141
                     /locus_tag="VIF63_02300"
     CDS             5930..7141
                     /locus_tag="VIF63_02300"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY8238242.1"
                     /translation="MADTRILLLGNAASDPLVKTLGRPGRALTRIHDPEQLAAVGTEH
                     DVIVLDVVPAPRTLADICREIRAIEGLAEAPILAISSTDEVEDRIRLLEAGADDVMVR
                     PVDERELDARVEALDLRHRRSRELRPSTLVAATKRPGRRLIVVYSPKGGVGTTTVAVN
                     VALAIAARDPDGVALVDFTLMGGHVATHLDLRPKLTIADLMRDGQGMISPEILRSTYL
                     TRHERGVLVLAGSVTPSITPLLTGEEVTRILEGVMTAVPIVVVDLGSHFDERVVTSLE
                     MADDVVMVVTPDFPALQAMHSFFEYLTESSSKAAEPTVVVNEVYALQTLTPGDIENAI
                     GRRVAIRIPYDPLLYLRAANQGTPVFAGAPTSQPARRYDQLAAVLLGEDAPAAEQAPR
                     RRGLAAIFGRS"
     gene            complement(7138..7479)
                     /locus_tag="VIF63_02305"
     CDS             complement(7138..7479)
                     /locus_tag="VIF63_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8238243.1"
                     /translation="MIVRVLTARVRAGKVASFNALFRKQVELLREQPGLEYLRFARRF
                     VADGEEEAVLFEEWRDSASLYGWVGPNLAEPRLVPGVRELVDELQVAHYEVLATGAED
                     DEAGRQIPESA"
     gene            complement(7599..7684)
                     /locus_tag="VIF63_02310"
     tRNA            complement(7599..7684)
                     /locus_tag="VIF63_02310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7649..7651),aa:Leu,seq:taa)
     gene            complement(7730..>8473)
                     /locus_tag="VIF63_02315"
     CDS             complement(7730..>8473)
                     /locus_tag="VIF63_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8238244.1"
                     /translation="YWGGEAVSIEAARDSNDSMAAAERAYPGRIKWFTSLPWQYPARA
                     VDELARSCDQGAAGVMVLANVAGRSLTVPAFEPIWAEIDRRALPVLVHPAEPPGSDLM
                     DMGSYDLSWSVGFMFDTTLAITRMVFDGFLDRFPNLRLIVAHGGAALPYLVGRFAKGD
                     EVELASRRRMTAHPLEYLRRMYYDCITYDLGALRYLVSVVGADRVLFGTDWPHQVHDV
                     AGSLANTAALPPDQRDAIRGSNALALFGL"
BASE COUNT         1364 a         2980 c         2906 g         1223 t
ORIGIN      
        1 ggctacaacg gcctcaaggc gatcctcggc aacctgtacg gcctcgacat caagtactac
       61 gtcgaggtcg acttcgaggg cttccgcaag gtggtcgatg ccctcggcgg cgtgaccgtc
      121 aacgtccagg tgccagtgct cgacaacaac ttcccgcaac ccaacagcca tcgcctccgc
      181 ctgttcgtcc ctgccggcgt gcagcacatg accgggtcgc aggcgctcgc ctacgcccgc
      241 tcgcggaaga gcacctcgga cttcgaacgc ggggcacgcc agcagcggct catcgtgtcg
      301 cttcgccagc agctcgacat cggccaggtc ctgcgcaaca tcgacccgct gtcggcggcc
      361 gtcgggaagt cgctgaagac cgacatcccg cgcgacctcg tgccacagct cctcggcctc
      421 gcggatcgca tcgacacgcg ctcgatccga tcggtgatct tcacgccgcc gttctaccag
      481 caggagaccc tgtcaggccc gcggggctac atcattcagc cccgtctcgc caggatccgt
      541 gccgcgatcg ccgaggcgtt cagcatcgat ccgggcttcg ccgaggcacg cgacgcgctg
      601 ctcgaggagg gcgccgagct gtgggtcctg aacggctccg ggcgcaacgg cgaggcggcg
      661 cgcctctcgg agtacctgag ctacctcggg ctcgctgcgt cggcgcccaa ccagaagccc
      721 gacacgacgg gcctcagcaa gaccacgatc cgcgtctaca acggcgccga ggaacgcttc
      781 ccgctgacac tcgctgccct gcagaaggtc ttcggggtca cggtcacgcc ggtcaccgac
      841 ccggccgtcc gcgtcgactt catcgtgatc accgccgatt cgacccccga gctgacggcg
      901 ccaccggcac cataacggaa cgtccgtccc gctcaggcgg atgcgggcca aggcgacggg
      961 gagcacccga gcgagcgcac ttcaacgtgc gtgagcgagg gcgcggaccc ggcaacgccg
     1021 gaccgcgccc gcatcagcgg gacggaattg ctgtgagcca gtgcatgaag cgctggtctc
     1081 ggccgcgcac tgccgcgaag taggcctcgt ggagctgctt ggtgatcggg ccgatggcgc
     1141 caccgccgat ggggcgatgg tcgagctcga tgaccggtga gagctggacg ccggtgccgc
     1201 agaggaacgt ctcgtcggcg acgtagatct ccgagcggtc gatcgagcgt gactcgtgcg
     1261 ggatgccgag cgcatcggcg agctcgagca cggcgcgacg cgtcacgccc tcgaggatgt
     1321 cgtcgttgac cggcggcgtc agcaggatgc catcgcgcac gacgaacagg ttcgcagccg
     1381 aagcctcgga cgcgtggccg tcgggggtaa ggacaagcgc ctcgtcgaag ccgttgagct
     1441 ccgcctcgga cttctggaac gccatgttca cgtagccgcc gacgatcttg ccgcgcgccg
     1501 gcagggcgtc gtcggcgttc cggcgccagg tcgaggtcat gacccggacg cccttgtcga
     1561 cgtcgatgta gttgccgaac gggacggcga gcacgaccag gtcgtggggc aggttgtgca
     1621 ggcgcacacc cacggacttg ccggacttgt acaggcacgg tcggatgtac gcgtcctcgc
     1681 ggaactcgtt gcggcggatc gtctcgacga cgatgcgcac gaactcgtcg accggcggca
     1741 ggtcggtcat gagcagcatc ccggcattgc gccggatgcg ctccatgtgc tcgcgcagct
     1801 tcagcccgta gagcacttcc tgctcttcgt tccagtacgc ccggatgccc tcgaagaccg
     1861 cggtcccgta catgaaggcg tgggtcatga tgttgacgtt cgcgtcccgc agcgggacgt
     1921 agttgccctc gaagtaggcg atgagcccgt cggtcgaggc gggtccgtgg gcggctgcag
     1981 gccgcgccga tgtctccgac ttggacttct cggtgagcat ctggacgggc tcctcggtgg
     2041 gtgcggcgca cgcgccgcag gtcggtcgat tcggtgcgca gtcggcgccg gtcgagtata
     2101 ccgcgagggc ctcgcggccc agcccatccg aagcggtttc cgggtgtggc tatccggttg
     2161 cgacccgtac ggcggtcgtc ctagccctgg ctgatcccga cgaccacgat cccgagatag
     2221 acgatataga acagcccgcc gacgagcagg ccgcgacctc gcagccggcc cgcgcggtac
     2281 atcggcagga agatgaaggc actcgccagg aaggcgatgc cggccgaggc gaacgcgagg
     2341 tagctgccct ccgcggccac ccatttctcc ggggcgaaga tgagcgccac cgacaccggg
     2401 atcgccgact ggaagaccat cgcaccggtg atgttgccca tcgcgagggt gtccttgccc
     2461 tggcggatcc agatgatcga gttgaacttc tccggcagct cggtcgcgat cggggcgacg
     2521 acgagggcga ggagcacctg gctgacgccg agcgccgtgg cgacatgccc gaccgcgtcg
     2581 acgaacgcaa tggcgcccac gatgatcagc ccgagggcga cgatcacctg gaagttcacg
     2641 gcccggagcc gcggcgcgtt gtcggagccg gtggtgcgac cggagtcgaa ccggtggaag
     2701 cgcaacggcg cgaggtcctc gacgtcgacc gtcgggtcgg cctcgaagtg ggccttcacg
     2761 taccaggcgt agacgagcag cagcgcgatc gcgacgacga tcttcggcca ggtcggctcc
     2821 gacggcagga agatggccgc ggcgatggcg aggccgtagg cgatcacgaa ggtccgcatg
     2881 tcggtgctga gcaccttcgt gtcgacctgc aggacgtcgc cggtctcccg gtgccgggcc
     2941 tgccagatca cggcgacgcc ggtcacgaac atcgcgagcg tcgccagcat gaacggggcg
     3001 ccaagaatgg cgccgacgcc gatctcgtcc gagtgggcct cggccgagaa caggatcgcc
     3061 acgatcggga tcatcgtctc gggcagcgcc gtcccgacgg cggccagcac ggagccgacc
     3121 gcgccctcgg cgaggccgag ctttcgcccg aaccattcga tgccgttcgt gaacagctcc
     3181 gcgcccgcga ggatgatcac gaacgaggcg gccaggatca ggaggtccat gtcccctagc
     3241 ctgtcaggag tccgaagaag acgcccacgg cgacgacgag caggatcgcc agcgacacga
     3301 gggccatcgt ccgctcgcca cggcgccaga agccgatgag cgcacgcagc acccgcaacg
     3361 cgggcgtcga caggacaccc acgatgccga tccacaggaa gcccgccgga cgcagtgcgg
     3421 cgaggtcggc gaggagcgtc ggcagggaga gcggcgggcc gcccgccgtc ggcgatgtcc
     3481 cgccggcgag cagcaggagc gtcccgagcc caaccaggcc gatggacacg taggtgccca
     3541 gctgcaggac atgggccagc gcaacctcca ggctgcccgg ccccgtcccc acggcgtcct
     3601 ccgctcgccc gctcattcga cgaccagcgc gcggcggccc atttcgaagg ccgtgaacaa
     3661 cagcaccacg acgaacagca ggcgcagcac ccggacatcg aggcgatggc cgaggcggga
     3721 cccgaggctg gccccgacga agacgccgat cgccgtcggg cccgtggcgt aggcgtcgat
     3781 cccgccgcgc aacaggtaga cgaacgcgct cgttgacgcg gtgatcccga ccatgaggtt
     3841 gctggtcgcg gtcgcgatac gcagcgggac accgagcccg agatgcatca acggcacctt
     3901 gatgagtccg ccaccgatgc ccaggagcgc cgaggcaacg ccagctccga ggctcgcaac
     3961 cgccgcgaac ggcatccgcc cgagcgccgc cggggcaagg ccgagcgtgg ccgcatccgg
     4021 gtcgaccgtc gcggcatcct catcggggac gtccccggct gcgggggcgc cttcgtccgc
     4081 cgtgatcggg gtggccggcc gccgcgcgct gcgcaggatc gtcgtcgcga catagacgag
     4141 caggatcgcg aacaggcccg cgagcagccg ctcgtcgacg aggaatgcaa tcgcgccacc
     4201 gatgatcgcc ccgatcccgg tgaacagctc gaggaccatc ccgaggcgga ggttggcctg
     4261 gcggcgctgc agatagacgc cggcggacgc gctgctcgtc acgatcacgc agacgagcga
     4321 gacgccgacc gcctcacgca acgggaggcc gaagccgatc gtgagcagag gcacgatgag
     4381 cagcccacca ccgagaccga gcagcgagcc gaacacgccg gcggcgaccc cgccgacgag
     4441 catcaggaga cctacctcca cgtcgtcagc ccccgtcgcc gctcaggcgg tcatcgcccg
     4501 ggggccgcat cagcggcgac taccggttca gctggcctag ggccggagat gttctggaga
     4561 gtaaggaaga agcgcgacgt ggcgcgccct cttgagggcc accgacagct cccgctggtg
     4621 gccggcgcag gtgccggtct tgcgtcgggg ctcgatcttc gcccgctccg agaggtagcg
     4681 gcggaggcgg ttgacctcct tgtaatcgat ctggacggtc ttgtcggcgc agaacgcgca
     4741 gaccttccgc cgccgtcgat cgcggtagaa gtcgtcgcgc ttggtgcggg agcgggcttg
     4801 ggctggaggc atctggtcag gttctcctgg aagtcgtggg gacgacggcg cctagaacgg
     4861 cagctcgtcg atctcggtgt cgtcgtagcc gccggaacgg ccgccgcctg cgggagcgcc
     4921 cgcgcccgcg ggagctccgg cgccagcgcc ggctggagca cctgcgaagg tgccctcggg
     4981 accggcctcg cgcttgtcga ggccgaggac cgtctcggcg gtgatgtcga gggacatgcg
     5041 cttctggccg tcgttggcct cgtactcccg cgtgcggaag cggccgtcga cgaagacgcg
     5101 actgcccttg cgcaggttct cggcggcacg ctcgccgcga tcaccccaga tgctgacccg
     5161 gaaccagtcc gtggactcga tccactcgcc ggactgctgg ttcttggtcg actggttgac
     5221 cgcgacggtg aagctcgcca cgggcctgcc gttgggcgta tatcgcagct cggggtcgcg
     5281 cccgaggttg ccgatgatct ggatcttgct cagcgccatc tgtcgaccct ctcagtcgat
     5341 cgcggccgga gccgcctcgc tctccgactc gtcgatccgc tcgcgcccat catcctcttc
     5401 ttcctcgtcc tccgcggacg gaagcagcgt ctcgtcggca tcgtccaggc cggaatcgcg
     5461 cgaggcgcgc agcggtcgat cctggcggac gatcaggtgt cgaagcagct cctcggtgat
     5521 gaggagggct cgctccacct cggcgatgac ggttgtcggc gcctcgaaca cgacgatgtg
     5581 gtaggcgccc tcacggtgcc ggtcgatggc ataggccagg cggcggcggc cccacggggc
     5641 gaccttcacg atctggccgc cgtcggtggt gatctggcgg gtggtgcgat cgatgatcgc
     5701 ggagatgcgg tcgtccggcg cgtccggccg gaataccagc atcagttcgt agcgacgcac
     5761 gggcgtcagg ctcctctctg tggggttgcc ccgggtcgac gacgtcgcac ggagcaggga
     5821 ggtcaggcgg cggagtctac catccccgac ccgttgaccc ccagaggctc gaagagcccg
     5881 ggctgtatcc tctggcggcg ccaacaccat gaacgaccca gggagacccg tggccgatac
     5941 tcgaatcctg ctcctcggca acgcggccag cgacccgctc gtcaagacgc tcggtcgccc
     6001 cggccgggcc ctgacccgga tccacgaccc cgagcagctc gctgcggtgg gcacggagca
     6061 cgacgtgatc gtgctcgacg tcgtgcctgc cccgcggacc ctggccgaca tctgccgcga
     6121 gatccgcgcg atcgagggcc tcgccgaggc gccgatcctc gccatcagct cgaccgacga
     6181 ggtcgaggat cggatccgcc tgctcgaggc cggcgcggac gacgtgatgg tccggccggt
     6241 cgacgagcgc gagctcgacg cccgggtcga ggcgctcgac ctgcggcacc gacgctccag
     6301 ggagctgcgc ccgagcaccc tcgtcgcggc gacgaagcgc ccgggccggc gcctgatcgt
     6361 cgtctactcg ccgaagggcg gcgtcggcac gaccacggtc gcggtcaacg tcgcgctcgc
     6421 gatcgctgcc cgagacccgg atggcgtcgc cctcgtcgac ttcacgctga tgggtggcca
     6481 cgtggcgacc cacctcgatc tccgccccaa gctgaccatc gccgacctca tgcgcgacgg
     6541 ccaggggatg atcagcccgg agatcctgcg gagcacgtac ctgacccgcc acgagcgcgg
     6601 cgtcctggtg ctcgccggct cggtgacccc gtcgatcacg cccttgctga ccggggagga
     6661 agtcacgcgc attctcgaag gcgtcatgac cgcggtgccg atcgtcgtcg tcgacctcgg
     6721 gtcgcacttc gacgagcgcg tcgtgacctc gctcgagatg gccgacgacg tggtcatggt
     6781 ggtgaccccg gacttcccgg cgctccaggc gatgcactcg ttcttcgagt acctcaccga
     6841 gtcgagctcg aaggccgccg agccgacggt cgtcgtcaac gaggtctacg ccctgcagac
     6901 gctcacccct ggcgacatcg agaacgccat cggtcggcga gtcgcgatcc ggattccgta
     6961 cgacccactg ctctacctgc gtgcggcgaa ccagggcacg ccggtgttcg ccggcgcccc
     7021 gacctcacag ccggcgcggc ggtacgacca gctcgccgcc gtgctgctgg gcgaggatgc
     7081 gcccgctgcc gagcaggctc cgcgccggcg cggcctcgcg gcgatcttcg gccgcagcta
     7141 ggcgctctcc gggatctggc gtcccgcttc gtcgtcctcc gcgcctgtcg cgagcacctc
     7201 gtagtgggcg acctgcagct cgtcgacaag ctcgcggacg ccgggcacaa gccttggctc
     7261 ggccaggttc gggccgaccc agccgtatag cgacgcggag tcccgccatt cctcgaacag
     7321 gactgcctcc tcctcgccgt cggccacgaa tcgccgcgca aagcggaggt actcgaggcc
     7381 cggctgctcg cggagcagct cgacctgctt cctgaagagc gcgttgaacg aggcgacctt
     7441 gcccgcacga acccgtgccg tcagaacccg gacgatcatc gcaagatgcc tctctcggac
     7501 cagcccacag tgcacgccag tctgccgaac cgtggggttg ccgccaagag gctgacattg
     7561 cggctgtgtg gcaaacgcgc ctccgcctgg cgcgagcctg gtgccgaagg ggagattcga
     7621 actcccacga ggtttccccc agccgatttt aagtcggccg cgtctgccct tccgccactc
     7681 cggcggcgtg agcgtaccgc ggctccccgg gcacgagccc ggcgcgcggt tacagcccga
     7741 agagcgcgag agcgttggat ccgcggattg cgtcgcgctg gtccggcggc agcgctgccg
     7801 tgtttgccag cgaccccgcc acgtcgtgga cctggtgcgg ccagtcggtg ccgaacagca
     7861 cgcggtccgc gccgaccacc gagaccaggt accggagcgc gccgaggtcg taggtgatgc
     7921 agtcgtagta catgcgccgc aggtactcga gcgggtgggc ggtcatccgg cgccggctcg
     7981 ccagctcgac ctcgtcgccc ttcgcgaaac gcccgacgag atacggcagt gctgcgcccc
     8041 cgtgcgccac gatcagccgc aggttcggga atcggtcgag gaacccgtcg aagaccatcc
     8101 gcgtgatcgc gagcgtggta tcgaacatga acccgaccga ccagctgagg tcgtagctgc
     8161 ccatatccat caggtccgag ccgggcggtt cagcgggatg gaccagcacc ggcagcgcgc
     8221 gtcgatcgat ctcggcccag atcggctcga acgctggcac tgtcaggctc cgacccgcca
     8281 cgttggcgag caccatcacg ccggcggcgc cctggtcgca gctgcgggca agctcgtcca
     8341 cggcccgagc cgggtactgc cacggcaggg aggtgaacca tttgatcctg cccggatacg
     8401 cgcgctccgc cgcggccatc gagtcgttgg aatcccgcgc cgcttcgatg ctcacggcct
     8461 cgccgcccca gta
//
LOCUS       DATBSW010000085         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_782358, whole genome shotgun sequence.
ACCESSION   DATBSW010000085 DATBSW010000000
VERSION     DATBSW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782358"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VIF63_02320"
     CDS             <1..577
                     /locus_tag="VIF63_02320"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEY8238245.1"
                     /translation="STKLAELGYELEGEALDAIYRQAIELADEKKEVTDDDLIALVEQ
                     RVAEVPQAVRLEGWNVTSSHGGKATGMVALSVGDEDHAKAAIGNGPVDALFEAVDAAL
                     EPVFGWHPVLETYEIKAVSGGEDAQGRVLVRCRRSSDEGPGALIVSGHGLSTNIIEAS
                     IEAYLVAVNKLHGAQSQASSAFVGARTTELP"
     gene            582..1721
                     /gene="leuB"
                     /locus_tag="VIF63_02325"
     CDS             582..1721
                     /gene="leuB"
                     /locus_tag="VIF63_02325"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY8238246.1"
                     /translation="MSTYRIASIGGDGVGPEVVAAGRRVLDAVATRDGFTVEWLDIVA
                     GGAAIDAYGVALRTEDLDAVAACDAVLLGAVGGPRWDDPDARVRPEQALFALRGGLEL
                     FANLRPVKVDPTLIDSSPVRPELLDSVDMLIVRELTSGLYFGRPSEQRDTPEGRVGID
                     TLWYTEGEIRRVVRLAFELAGGRRRRLTSVDKANVLATSRLWRKVVDETRPEFPDVEV
                     NHQLVDSCAMLLIRKPTQFDVLVTENLFGDILSDEAAVLAGSLGMLPSASVGERRTAH
                     GRFGLYEPIHGSAPDIAGKDLANPLGTVLSAAMLLRWSLGLESAAADVEAAVGAAIAD
                     GYRTADLVPPDGDRSGLRVVGTNDMTAAILERMPARVGATTGAAT"
     gene            1718..>2067
                     /locus_tag="VIF63_02330"
     CDS             1718..>2067
                     /locus_tag="VIF63_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238247.1"
                     /translation="MSETPVVLYDTTLRDGTQGENITLSLADKLRVARLLDEYGMPYV
                     EGGWPGSNPKDIEFFKAAKDMRWATAKLAAFGSTRHRSNTAQSDANLTELLRAETPAV
                     TIFGKSWELHVVEVL"
BASE COUNT          311 a          732 c          735 g          289 t
ORIGIN      
        1 ctcgaccaag ctcgcggagc tcggctacga gctcgagggc gaggccctcg acgcgatcta
       61 ccgccaggcg atcgagctcg ccgacgagaa gaaggaggtc accgacgacg acctcatcgc
      121 cctcgtcgag cagcgcgtcg ccgaggtccc ccaggccgtc cggctcgagg gctggaacgt
      181 gacgtcgtcc cacggcggca aggcgaccgg catggtcgcg ctgtcggtcg gcgacgagga
      241 ccacgccaag gccgcgatcg gcaacgggcc ggtcgacgcc ctgttcgagg ccgtcgatgc
      301 ggcgctcgag ccggtgttcg gctggcatcc cgtgctcgag acctacgaga tcaaggcggt
      361 gtccggcggc gaggacgccc agggccgcgt gctcgtgcgc tgtcgccgct ccagcgacga
      421 gggcccgggc gcgctgatcg tgtccggcca tgggttaagc acgaacatca tcgaggcctc
      481 gatcgaggcg tacctcgtcg cggtcaacaa gctccacggc gcgcagagcc aggcgtcctc
      541 cgcgttcgtc ggcgcccgaa cgaccgagct gccgtagccg cgtgagcacg taccgaatcg
      601 catcgatcgg gggagacgga gtcggcccgg aggtggtcgc ggctgggcgt cgcgtgctcg
      661 acgcggtcgc gacgcgcgac gggttcacgg tcgaatggct cgacatcgtg gccggcgggg
      721 cggcgatcga tgcctacggc gtggcgctcc gcaccgagga tctcgacgcg gtcgcagcgt
      781 gcgacgcggt cctcctcgga gcggtcggcg gacctcgctg ggacgacccg gacgcgcgcg
      841 tccggcccga gcaggcgttg ttcgcgctgc gcggcggcct cgagctcttc gcgaacctgc
      901 ggccggtcaa ggtcgaccca accctcatcg actcgtcgcc cgtccgaccg gagctgctcg
      961 acagcgtgga catgctgatc gttcgggagc tgacctcagg gctgtacttc ggccggccgt
     1021 ccgagcagcg ggatacgccc gagggtcgcg tcggcatcga cacgctctgg tacacggaag
     1081 gggagatccg gcgcgtcgtc cggctcgcgt tcgagctcgc cggaggccgc cgccgccgcc
     1141 tcacgagcgt cgacaaggcc aacgtcctcg cgacgtcccg gctgtggcgc aaggtcgtcg
     1201 acgagacccg accggagttc ccggatgtcg aggtcaacca ccagctcgtc gactcgtgcg
     1261 ccatgctcct gatccggaag cccacccagt tcgacgtcct cgtgaccgag aacctcttcg
     1321 gcgacatcct gtcggacgag gccgccgtgc ttgcgggcag cctcggcatg ctcccgtcgg
     1381 cgtcggtggg ggagcggcgg accgcgcacg ggcggttcgg cctgtatgag cccatccacg
     1441 gttccgcgcc ggacatcgcc ggcaaggacc tggcgaatcc actcggaacg gtcctgtcgg
     1501 ccgcgatgct cctgcgctgg tcgctcgggc tggaatcggc ggccgcggac gtcgaggcgg
     1561 cggtcggcgc ggcgatcgcg gacggctatc gaacggccga cctcgtgccg cccgacggcg
     1621 accgctccgg cctccgagtc gtcggcacaa acgacatgac cgcggcgatc ctcgagcgaa
     1681 tgccggcgcg cgtgggcgcg acgacgggag cggccacgtg agcgagacgc cggttgtcct
     1741 ctacgacacg acactccgcg acggtacgca gggcgagaac atcacgctct cgctcgccga
     1801 caagctgcgc gtcgcgcgcc tcctcgacga gtacggcatg ccctacgtcg agggcggctg
     1861 gcccggctcg aacccgaagg acatcgagtt cttcaaggcc gcgaaggaca tgcgctgggc
     1921 gacggcgaag ctcgcggcgt tcggctcgac ccgccatcgc tcgaacaccg cgcagtccga
     1981 cgcgaatctc accgagctcc tccgcgcgga gacgcccgcg gtcaccatct tcggcaagag
     2041 ctgggagctc cacgtcgtcg aggtcct
//
LOCUS       DATBSW010000086         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_430008, whole genome shotgun sequence.
ACCESSION   DATBSW010000086 DATBSW010000000
VERSION     DATBSW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430008"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..1963
                     /gene="dnaA"
                     /locus_tag="VIF63_02335"
     CDS             482..1963
                     /gene="dnaA"
                     /locus_tag="VIF63_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY8238248.1"
                     /translation="MDAKQVWRAALGELQVTLSPANFETWLRDTALVEVDDTRFRISV
                     PNGFAKDWLESRYRSLISQTLGRIVGYGVQVDFFVGAAETPEGETADTAAGEGETEAI
                     RMDEPAKAAARVAVGPGMLPRSGDAPERRQAPSSPQVRVEPGRVGGGEGGTSNINTRY
                     TFSNFIVGSANRLAHAASLSVAERPGHAYNPLFLYGGVGLGKTHLMHAIGNQVAAKFP
                     RKRVVYATSEKFTNEFITSIQQGKIDEFRARYRRIDLLLIDDIQFIADKERTQEEFFH
                     TFNAIHEDGKQIVLSSDRPPKAILTLEERLRSRFEWGLIADLTAPDLETRIAILRAKA
                     EEGAVPISSEVIEFIARKVVSNIRELEGALNRIVAYASMGAMPISIELAQAVLSNVLY
                     NPKKRQITPERIVKAVSEYYGVGLDALKGQKRDKAIVVPRQIAMFLMREETDVSLLRI
                     GAELGNRDHSTVLHACDKITRESGGNDELRREIAAVRELIYAD"
BASE COUNT          331 a          724 c          663 g          308 t
ORIGIN      
        1 cattcgggcg cggaagccgt gttccttggc gcggtggcgc ttcttgggct ggtaggtctg
       61 cttcgtcatc gtgcctgcgc gggatcctct ccatccggcc tcggggtagt ccgttatcga
      121 ccccagaagg cacgcaaacg cgccaaacac ccgaagcacg ggtggggcgc gtcaacgtcc
      181 ggcggatgat aggtcgcgcg gatcgggacc gtcaaacgtg acacgctcgc gggagccctt
      241 cgagcgccgt tcgccggcgc ctccgcgacc cttgcaccgg tccgggcagc caggatgctg
      301 atactccccg ggggtatcgc ggattcgcct tgcggggtcg cggcccgcgg tgctatgttt
      361 cgccacccga agccccggcg atcggccacg ttgccccgcg ccgagcctcg gtcgcgtgcc
      421 ctttttgccc ccgcgggcgt caatttccca ggccccggaa cacgagagtc ctatcgatcc
      481 tatggatgcc aagcaggtct ggcgcgcggc cctcggcgag ctccaggtca ccctctcccc
      541 cgcgaacttc gagacgtggc tgcgcgacac cgcgctcgtc gaggtcgacg acacgcgctt
      601 ccggatctcg gtgccgaacg gcttcgccaa ggactggctc gagagccgct accgctccct
      661 gatctcccag accctcggtc ggatcgtggg ctatggcgtc caggtcgact tcttcgtcgg
      721 ggcggccgaa acgcccgagg gcgaaaccgc ggacaccgcc gccggcgagg gcgagaccga
      781 agccatccgc atggacgagc cggcgaaggc cgctgcgcga gtcgcggtcg ggcccgggat
      841 gctcccccgc agcggcgacg ccccggagcg gcgccaggcg ccgtcgtcac cccaggtccg
      901 ggtcgagccc ggccgcgtcg gtggcggcga gggcggcacg agcaacatca acacccgcta
      961 cacgttctcg aacttcatcg tcgggtcggc caaccgcctc gcccacgcgg cgagcctgtc
     1021 ggtggcggag cgccccgggc acgcgtacaa cccgctcttc ctgtacggcg gcgtgggcct
     1081 cggcaagacc cacctgatgc atgcgatcgg caaccaggtc gcggcgaagt tcccgcgcaa
     1141 gcgcgtcgtc tacgcgacgt ccgagaagtt caccaacgag ttcatcacct cgatccagca
     1201 gggcaagatc gacgagttcc gcgcccgcta ccgccgaatc gacctgctcc tcatcgacga
     1261 catccagttc atcgccgaca aggaacggac ccaggaggag ttcttccaca cgttcaatgc
     1321 catccacgag gacggcaaac agatcgtgct gagctccgac cggccgccca aggcgatcct
     1381 gaccctcgag gaacggctcc ggtcccgctt cgagtggggc ctcatcgccg acctcaccgc
     1441 gccggacctc gaaacgcgca tcgccatcct ccgggccaag gcggaggagg gcgcggtccc
     1501 gatctcgtcc gaggtcatcg agttcatcgc ccgcaaggtc gtgtcgaaca tccgcgagct
     1561 cgagggcgcg ctgaaccgga tcgtcgcgta cgcctcgatg ggcgccatgc cgatctcgat
     1621 cgagctggcc caggccgtgc tctcgaacgt cctgtacaac ccgaagaagc gccagatcac
     1681 gcccgagcgc atcgtcaagg cggtctccga gtactacggc gtcgggctcg atgcgctcaa
     1741 gggccagaag cgggacaagg cgatcgtcgt gccccgccag atcgcgatgt tcctgatgcg
     1801 cgaggagacg gatgtctcgc tcctgcgcat cggcgccgag ctcggcaacc gcgaccactc
     1861 gaccgtgctc catgcgtgcg acaagatcac ccgcgagtcg ggcgggaacg acgagctccg
     1921 gcgcgagatc gccgcggtcc gcgagctgat ctacgccgac tgatcgctcg gggcgttttc
     1981 cacggaacgg cccgggtatg tggagatccg gtggggtcgc aaggtc
//
LOCUS       DATBSW010000087         5693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_782650, whole genome shotgun sequence.
ACCESSION   DATBSW010000087 DATBSW010000000
VERSION     DATBSW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782650"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VIF63_02340"
     CDS             <1..235
                     /locus_tag="VIF63_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8238249.1"
                     /translation="PADQLGAEARSVAARLAAGAPRAIALTKRALNAAWDRDLDAALE
                     YEAHLQDMAGRSKDHAEGLAAFMEKRSPRFTGE"
     gene            complement(248..1564)
                     /locus_tag="VIF63_02345"
     CDS             complement(248..1564)
                     /locus_tag="VIF63_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238250.1"
                     /translation="MRPDDRRADLARPVGDEPLDAPASPLDASLAAVDAELSAAGAQA
                     RRALHGRTQPTRYFDGELRERLLGGYGAPLAAGLGADAALPRDSWRGSRVRPEIDRTG
                     ETWAPTPLTPRISRRTPTVMPRARWALLAAAALSGVLVVGALGARPDWLLPVPTAAPS
                     APPASSSPETPPPSAAPTEAVVAPVESPATSAPPVSEPTKPPATSKPEPTANPTPKPT
                     HKADPTPKPEPTTPPVGPMGLAAKACPGGVVLDWTKPSTAATHYHVLRSLEGDVPATW
                     PTDGGDDVESATSWSAGITDGFDAGLDGGTSVTYRAFAFDDEDHFLASSPSRTVTTVA
                     ALSLGEPGIVEDGPGSITVSWSGADLAEGCFSYGKLVASEEDPDPSYMKGSPYLVVVE
                     NPDTTSVSLDGLPSGKTVWLRYELIRVTGTGKFIAGGTDVAQVTFP"
     gene            complement(1554..2159)
                     /locus_tag="VIF63_02350"
     CDS             complement(1554..2159)
                     /locus_tag="VIF63_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8238251.1"
                     /translation="MDPAREQALVARARADPAAFGELYDFYLPRIYGYVARRVEDRSV
                     AEDLTATTFERALGAVRGEDFRNASFGGFLYRVASNAVVDHVRRARRTIPLGMRASDL
                     DDVDSQRGRSDRRFDAAAISDESAARAFSAAVDRDTLRRALQRVPDVHRRVILLKYFD
                     GLELDELCAALGCSRGTFAVKLHRALRALRAAVEQEATDAA"
     gene            complement(2298..3488)
                     /locus_tag="VIF63_02355"
     CDS             complement(2298..3488)
                     /locus_tag="VIF63_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238252.1"
                     /translation="MIAAPIVPAIEPAVELLGDRSVPIEGTHRGLPLGRSLSGAVVGT
                     LLVVGLALGAALGPGGALGPASDRDGSTSGDTAAAGHPGDGDGSYGNGTNSDAGSGEG
                     TSDDEDEWHGGPDATDKPDAGEGGNEEHPDATDKPDAADGDEGGDAEHPDATDKPDVE
                     PTEKPDPTPKPTDKPDATKVPTEPIALGLAIKEAHPVIEWGSCGDLDFDVYKVVRSTN
                     SSVSWPMGEGDELVAAIERGGARRAYDESAPHGDKVWYRVFCVRKGEAGYKVVNASVT
                     KGIEVPEEPAPPTPPDPITLGLEVSSADGGKILLDWSACEVDGFAFYKVLRSTTTEDP
                     SYLPWHEGTDVIGVIESMDNTQLAAWAPDGDQTALYRVQCIGYSGDTKVLLGESAVVA
                     VTVP"
     gene            complement(3670..4050)
                     /locus_tag="VIF63_02360"
     CDS             complement(3670..4050)
                     /locus_tag="VIF63_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8238253.1"
                     /translation="MRYFLDTTFAIDYLRGLAVAEQRLARMFESGDEPYVNEVVLCEL
                     AVGLRDAGDPAFIAFTQAISFVQPSAEIAVQAGVWRRDAARRGRTLDLADALIAAAAD
                     GLDAPVLTRNLRDFALTPVKVEGY"
     gene            complement(4054..4308)
                     /locus_tag="VIF63_02365"
     CDS             complement(4054..4308)
                     /locus_tag="VIF63_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238254.1"
                     /translation="MSVRTNLLLPVDVVEGLDRVAGPRGRSRYVAEAVRERIRRDERM
                     AAIRAAAGIWRDHPEFPTDDSVVDWVRRLRREETDSGPEP"
     gene            4411..5004
                     /locus_tag="VIF63_02370"
     CDS             4411..5004
                     /locus_tag="VIF63_02370"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8238255.1"
                     /translation="MVELTRRQAIEDVASDLFREHGYAGTSIRDIARALSVQGASLYA
                     HVTSKEDVLWAIVDRAASRFEAAADAAETDAEARRPTDPIEALAALVRAHVRVLTRDV
                     DEASVFVHEWRSLGAERRAVILARRDAYQARFRTKIEAGIAIGAFALTDPSIAASTIL
                     SALNGIAAWYDPDGRLPADRIADHLVDLSMHMLEASR"
     gene            5001..>5693
                     /gene="paaA"
                     /locus_tag="VIF63_02375"
     CDS             5001..>5693
                     /gene="paaA"
                     /locus_tag="VIF63_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY8238256.1"
                     /translation="MTDPLALDPVLPVRQRSERPWNEDLPHDERYAHFEAHVTAGGKI
                     ESTDWMPDEYRTAVLRFVEMHANSELMGVLPEREWIMRAPTLRRKLALTAKVQDEVGH
                     AQLLYRVAEDLGKPRESMLEDLIAGKTKFHNVFHYPTKSWGDIGIIAWLVDAAAIVAQ
                     QALRDSSYAPYARTMQKICWEESVHIMHGRDVVVTLMTGTDSQRALVQEALDRWWGPL
                     MQMHGPRGDRAKD"
BASE COUNT          823 a         2018 c         2013 g          839 t
ORIGIN      
        1 gcccgccgac cagctgggcg cggaggcgag atccgtcgct gcccgtctcg cggccggcgc
       61 gccgcgcgcc atcgcgctga cgaagcgcgc cctcaacgcc gcgtgggacc gcgacctcga
      121 cgccgcgctc gagtacgagg cgcacctcca ggacatggcc ggccggtcga aggaccacgc
      181 cgagggactc gcggcgttca tggagaagcg gtcaccccgg ttcaccggcg agtagttcgc
      241 cgccggtcta ggggaacgtg acctgcgcga cgtcggtgcc accggcgatg aacttgccgg
      301 tgccggtcac ccggatcagc tcgtagcgca gccagaccgt cttgcccgac ggcagtccat
      361 cgaggctgac gctggtcgtg tccgggtttt cgacaaccac caggtatggc gagcccttca
      421 tgtagctcgg gtcggggtcc tcttccgacg ccacgagctt gccgtagctg aagcacccct
      481 cggcgaggtc ggcgccagac caggagacgg tgatcgagcc cgggccgtcc tcgacgatgc
      541 ccggctcgcc gagcgagagc gccgcgaccg tggtcacggt gcgggacggg ctcgacgcga
      601 ggaagtggtc ttcatcgtcg aaggcaaacg cccgatacgt cacgctggtc ccgccatcga
      661 gcccggcgtc gaagccgtcg gtgatgccgg cgctccagct cgtcgccgac tcgacgtcgt
      721 cgccgccatc ggtcggccac gtggcgggca cgtcgccttc gaggctgcgg agcacgtggt
      781 agtgcgtcgc ggcggtcgag ggcttcgtcc agtcgaggac gacaccaccc gggcaggcct
      841 tcgcggcgag gcccatcggg cccaccggcg gcgtcgtcgg ctcgggcttc ggggtcgggt
      901 cggccttgtg ggtcggtttc ggcgttgggt tggcggttgg ctccggcttc gaggtggcgg
      961 gaggcttcgt gggctcgctc accggaggcg cggaggtcgc cggcgattcg acgggcgcca
     1021 cgacggcctc ggtcggagcg gccgagggcg ggggcgtttc cgggctcgag gatgcgggcg
     1081 gcgcgctcgg ggcggcagtg ggcaccggca gcagccagtc gggacgcgcg ccgagggcgc
     1141 cgacgacgag caccccggac agcgcggccg cggcgaggag ggcccaccgt gcgcggggca
     1201 tgaccgtcgg cgtacgccgc gagatgcgcg gcgtcagcgg cgttggcgcc caggtctcgc
     1261 cggttcgatc gatctcaggg cgcacgcgcg agcctcgcca cgaatctcgc gggagtgccg
     1321 catccgcgcc gaggccagcc gccaggggcg cgccgtaccc gccgaggagg cgctcgcgca
     1381 gctcaccgtc gaagtagcgc gttggctgcg tccggccgtg gagcgcgcgt cgcgcctgcg
     1441 cgccggccgc gctgagctcg gcgtcgactg ccgcgaggct tgcgtcgagc gggctcgccg
     1501 gggcatcgag cggctcgtcg cccacggggc gagcgaggtc ggcgcggcgg tcgtcaggcc
     1561 gcatcggtcg cctcctgctc gactgcggcg cgcagggcgc gcagggcacg gtgcagcttg
     1621 accgcgaacg tgccgcgcga gcagccgagt gcggcgcaca gctcgtcgag ctcgagtccg
     1681 tcgaagtact tgagcaggat cacccggcgg tgcacatccg gcacccgctg gagcgctcgg
     1741 cggagcgtgt cccggtcgac ggccgccgag aatgcgcggg cagcggactc gtcgctgatc
     1801 gccgcggcat cgaagcgacg gtcggatcgc ccacgctgcg aatcgacgtc gtccaggtcg
     1861 ctcgcccgca tcccaagggg aatcgtccgg cgcgcccgac gcacgtggtc gacgaccgcg
     1921 ttcgacgcga cccggtacag gaatccgccg aacgacgcgt tgcggaagtc ctcgccgcgc
     1981 accgcgccga gcgcccgctc gaacgtcgtc gcggtcaggt cttcggccac ggaccggtcc
     2041 tcgactcgac gcgccacgta gccgtagatg cggggcaggt agaagtcgta cagctcgccg
     2101 aaggcagccg gatccgcccg ggcgcgggcc acgagggcct gctcccgagc gggatccatg
     2161 cgcgttccat gtccatcaat tgccatcggc ggcccgaacg tacgggtgga ccgtccatcc
     2221 ggccatcgcc cagcggtact cccgccgatg cgccaaccag gacgttcggt ccgcccattc
     2281 gtcgacctgg accgggccta cggcaccgtc acggcgacga cggcactctc accgaggagc
     2341 accttcgtgt cgccgctgta gccgatgcac tggacccggt acaaggccgt ctggtcgcca
     2401 tccggcgccc acgccgcgag ctgggtgttg tccatcgact cgatgacgcc gatcacgtcg
     2461 gtgccttcgt gccagggcag gtacgagggg tcctcggtcg tcgtcgagcg caggaccttg
     2521 tagaaggcga acccgtcgac ctcgcaggcg ctccagtcga gcaggatctt gccgccgtcg
     2581 gccgacgaga cctcgaggcc gagggtgatc ggatcgggcg gggtcggcgg cgccggctcc
     2641 tcgggcacct cgatgccctt ggtcacgctg gcgttcacga ccttgtagcc agcctcgccc
     2701 ttgcggacgc agaagacccg ataccagacc ttgtcgccgt ggggcgcgga ctcgtcgtag
     2761 gccctgcgcg cgccaccgcg ctcgatggct gcgacgagct cgtcgccttc gcccatcggc
     2821 cagctgaccg acgagttcgt cgagcggacg accttgtaga cgtcgaagtc gaggtcgccg
     2881 cacgagcccc actcgatgac cgggtgggct tccttgatcg ccagtccgag cgcgatcggc
     2941 tcggtcggca ccttggtggc gtccggcttg tcggtcggct tcggcgtcgg atctggcttc
     3001 tcggtcggct cgacgtcggg cttgtccgtg gcgtccggat gctcggcgtc gccgccttcg
     3061 tcgccgtcgg ccgcgtcagg cttgtccgtg gcgtccggat gctcctcgtt gccaccctca
     3121 cccgcatcgg gcttgtccgt ggcgtccggg ccgccatgcc attcgtcctc gtcgtcactg
     3181 gtgccttcgc cgctgccggc gtccgaattg gtcccgttgc cataggaccc gtcgccgtcg
     3241 ccgggatgac ccgcggcagc cgtgtcgccg ctcgtcgagc catcacgatc gctcgccggg
     3301 cccagggcgc cgcccgggcc gagcgcggcc ccgagtgcga gcccgacgac caggagcgtc
     3361 ccgacgaccg cgcccgagag cgatcgtccg agcggcaggc ctcggtgcgt gccctcgatc
     3421 gggaccgacc gatccccgag cagctcgacg gccggctcga tggccggcac gatcggcgcc
     3481 gcgatcacct gtgtatcgac cgcctgtgtc ggccgggccc gcgctgggct ccgcttgcga
     3541 ccagttccgc tcattccccg aacctccccg tgcaggccgc gatgtgcggc cgatgagacc
     3601 gatgtcctct aaacggggcg ggtgcctcgg gattaccgaa gcgtcggaag aagatcgtcc
     3661 tctctcggcc tagtacccct cgaccttcac gggcgtgagc gcgaaatcgc gcagatttcg
     3721 ggtgagcacg ggagcatcca ggccgtccgc agcagctgcg atgagggcat ccgcgaggtc
     3781 gagggttctg cctcgtcttg cggcgtcgcg ccgccaaacg ccggcctgaa cggcgatctc
     3841 ggccgacggc tgcacgaacg agattgcctg cgtgaacgca atgaaggcag ggtcgcccgc
     3901 gtcacggagc ccgacggcca gctcacagag gacgacctcg ttcacgtacg gctcgtcgcc
     3961 gctctcgaac atgcgcgcca ggcgttgctc cgcaacggcg agcccccgga ggtagtcgat
     4021 ggcgaaggtc gtatcgagga agtagcgcat cgcctacggc tcgggccccg aatcggtttc
     4081 ctcgcgtcgg aggcggcgca cccagtccac gaccgaatcg tccgtgggaa attccgggtg
     4141 atcacgccag attcccgcgg cggcccggat ggcggccatc cgctcgtcgc gacggatgcg
     4201 ctcgcggacc gcttcggcga cgtagcggct gcgacctcga ggtccggcca cgcggtcgag
     4261 cccttcgacg acatcaaccg gcagcaggag gttcgtgcgc acactcatgc gcacagtatt
     4321 gcgcatgcgt ggattgcgga caagggttgt tgacgatccg gcgcaggcga acctaaccta
     4381 acgatcgtta gttaggagac gccccgaacc gtggtcgagc tcacccggcg ccaggcaatc
     4441 gaagacgtcg ccagcgacct gtttcgcgag cacggctacg ccggcacgag catccgcgac
     4501 atcgctcggg cgctgtccgt gcagggcgcg agcctgtacg cgcacgtgac ctcgaaggaa
     4561 gacgtcctgt gggcgatcgt cgatcgcgcg gcgtcccgtt tcgaggctgc cgccgacgcc
     4621 gccgaaacgg acgccgaggc gcgccggccc acagacccga tcgaagccct cgctgccctc
     4681 gttcgagccc acgtccgtgt gctcacgcgc gacgtcgacg aggcgagcgt cttcgtccac
     4741 gagtggcgct ccctcggcgc cgagcggcgc gcagtcatcc tcgcccggcg cgacgcctac
     4801 caggcccgct tccgaacgaa gatcgaggcc ggcatcgcca tcggcgcctt cgcgctgacc
     4861 gacccctcga tcgcggcgag cacgatcctg agcgccctga acgggatcgc cgcctggtac
     4921 gaccccgatg gccgcctccc ggcggaccga attgccgacc acctggtcga cctgtcgatg
     4981 cacatgctgg aggcgtcccg atgaccgatc cgctcgccct cgatcccgtc ctgcccgtgc
     5041 gccagcggag cgagcgaccg tggaacgagg acctgcccca cgacgagcgt tacgcgcact
     5101 tcgaggcgca cgtcaccgcc ggcggcaaga tcgagtcgac cgactggatg cccgacgaat
     5161 atcggacggc cgtcctgcgc ttcgttgaga tgcacgccaa ctcggagctc atgggcgtgc
     5221 tcccggagcg cgagtggatc atgcgcgcgc cgacgctccg tcggaagctc gcgctcacgg
     5281 cgaaggtcca ggacgaggtc gggcacgcgc agctcctgta tcgcgtcgcc gaggacctgg
     5341 gcaagccgcg cgagtcgatg ctcgaggacc tgatcgcggg caagacgaag ttccacaacg
     5401 tcttccatta cccgacgaag agctggggcg acatcggcat catcgcctgg ctcgtcgacg
     5461 cggcggcgat cgtcgcccag caggcgctgc gcgactcgag ctacgcgccg tacgcgcgga
     5521 cgatgcagaa gatctgctgg gaggagtcgg tccacatcat gcacggccgc gacgtcgtcg
     5581 tgacgttgat gaccggcacg gactcgcagc gcgcgctcgt ccaggaggcg ctcgaccggt
     5641 ggtggggccc gctcatgcag atgcacggtc cgcgcggcga tcgggcaaag gac
//
LOCUS       DATBSW010000088        10047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1241200, whole genome shotgun sequence.
ACCESSION   DATBSW010000088 DATBSW010000000
VERSION     DATBSW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241200"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VIF63_02380"
     CDS             complement(<1..857)
                     /locus_tag="VIF63_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238257.1"
                     /translation="MTEILTESFCERCGTRYTFETVSSRRKPLSRIGTLGRGIRHFVS
                     DPGSSLDEAFAVARSETEQRSTATALEAFHRTFQFCLSCRQYTCGDCWNVVEGRCLSC
                     APTPDAPPPETRVDTAVSLQPMDLARAPAIIVAEPIIDHAAVAEAEAQAAAEAQAAAD
                     AESAAFAEAEAAAVAASEAAAAAQAAADAAAAAQAEAEAAAVAQAEAEAAAQTEADEA
                     ARIAAETEAREIAEAEARAAAEAEAAARRAEAEAAAIASIQEADARAAAEEAARERAA
                     AEARAIAEA"
     gene            984..1748
                     /locus_tag="VIF63_02385"
     CDS             984..1748
                     /locus_tag="VIF63_02385"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEY8238258.1"
                     /translation="MRAIARVERFIERLVERPSARLFRTRLQPVQVLRHIERVMEAGR
                     GAEGTRGLVPDQFVVKLHPEDLAAFLPADELASELASGALRFARAHGYALRDRPRVTL
                     QPNPDLRPAEVDVEAEVSPLAGQADGGERVDGGTRVFEVPVVRAPQVVIEVREPNRKP
                     RRVPMTGAPVHIGRAPECQLVLKDSRVSRRHARLHARDGVLVLTDLGSTNGTRVNGHR
                     VTELVLGAGDRIQIGETSLVVEAAPSEAPVGTIARP"
     gene            1761..2198
                     /locus_tag="VIF63_02390"
     CDS             1761..2198
                     /locus_tag="VIF63_02390"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8238259.1"
                     /translation="MDAAVLAIWVLRLLFLGLLYFFLWSVVRALLRDLRAAAREPTRE
                     LGRLVVVASPGGEPEAGAVFALDAITSLGRDVNNSIVLDDDFVSAAHAALTYRGRAWY
                     VEDLGSTNGTFVNGSQVDGLSPIAFGDEIQIGQVRMRLERARA"
     gene            2195..3676
                     /locus_tag="VIF63_02395"
     CDS             2195..3676
                     /locus_tag="VIF63_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8238260.1"
                     /translation="MTSAGVTSAGVTTARRLRTVGRGGRRFRITPRLRGRELGLLLLC
                     GVALFVGSWSLGATQAFRAAQLDGDTGSLNLSEAAEPGLLTIYLLALFGVHAALVLAG
                     RRTDQVLLPAVGMLGGIGLLLMIRLPQDLAGRLGGLAQTQLVWLVIGLVIIAGLGVAV
                     RNDVWLRRYKYTWAAVGVGLLLLVFLFGRDINGARLSLTIGPFSGQPSELLKVILVVF
                     LAGYLSENRGLLVEESTRIGPLRLPPLPYLAPMVAMWAIALGIVIVQRDLGAALLFFA
                     VFLLLLYVSTARVSFVVLGLAAFVVGAGALALVFDHVRTRIDIWLDPFADPSGAGYQV
                     VQALHAFARGGLIGTGLGGGLPTIAGRPPIPALHTDFPFAALGEELGLVGILAILGLY
                     LVLVERGLRIASAAQDEFRAILAAGLSLVIGVQAFIIAGGNLKLIPLTGITLPFVSYG
                     GSSLLANSVIVGLLIALSERTREPLPPPRRAGRLRRMLDRGAA"
     gene            3676..5271
                     /locus_tag="VIF63_02400"
     CDS             3676..5271
                     /locus_tag="VIF63_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8238261.1"
                     /translation="MPGEPQPERAPAPRERAPASRKPVHAYPISGSAPSWTGRRVGIG
                     AALNRVALALAVAFGGLALGAGYWQVIESSNLSTAGDDAAVIAAARHVKRGEIFDRDG
                     VRLAWNKVDKNGEPYRVYASNTLSGVIGYSSRQYGTAGLESAWNAELSGAVSADPLRE
                     LTRKFRADPSDPQDIKTTLVLELQKAAVAALGSKKGAVVLLDPRTGEVLALVSTPTFD
                     ASDVANPATSTRAFDALRTDDRLPLLPRATSGLYVPGSVFKIVTAVAALGSGAVTPDT
                     RYEDQPGSEKKGWLVDGFRVQDGHHTATGSKALNFAEAVEVSCNIWFAETGVRTGGES
                     LAVWAQRLGFGAPIPFDLPTKASQVTNGGGSFGGGFADRIELANAAYGQGETLVTPLQ
                     MALVAATIANDGVLMRPHLMLEATGKSGTTTVNPSTLERVIAPGVAEEIGAAMRLAVS
                     GQLGRVYTSGASVRGLAVAGKSGTAELDPGRAPHSWFIGFAPYDDPQVAIAVLVEGSG
                     SGGVRASPLAGQILRAWRDWANP"
     gene            5331..5498
                     /locus_tag="VIF63_02405"
     CDS             5331..5498
                     /locus_tag="VIF63_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238262.1"
                     /translation="MIERLGLALIALLLAGMFGALGYVSLTGGEVFLGIMGVIGALMT
                     VWAAASSLRRG"
     gene            5583..6587
                     /locus_tag="VIF63_02410"
     CDS             5583..6587
                     /locus_tag="VIF63_02410"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8238263.1"
                     /translation="MSRIQETGDRVLANVERVIVGKHNEVRFALVALLCKGHLLIEDV
                     PGTGKTVLAKAIAKSLGSSFRRIQFTPDLLPSDVTGLSIYNQKTTEFEFRPGPIMAQV
                     VLADEINRATPKTQSALLECMEERQATVDGITHMMPDPFLVIATQNPIEYEGTFALPE
                     AQLDRFMLRIRLGYPKPVEEVVILDEQKREHPIDDLEVVCSVEELQEMQRAVREIYVD
                     PTVSEYIVRLVNGTRDHGDIYLGASPRGSIALYRAGQALAGLLGRDYVIPDDVKALAE
                     AALAHRMIIKTSSSIHDVQPAQVIRELLESTSVGAVKEPAVAGLSEAKAGVRTRSDQG
                     "
     gene            6633..7973
                     /locus_tag="VIF63_02415"
     CDS             6633..7973
                     /locus_tag="VIF63_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8238264.1"
                     /translation="MFRRLQLLILAGILVIAAFSSGKDFLFYLVYLGVLVIGGSYVLT
                     RLGLADLEAGYAVSQLSGHVGDRIQVTYTLRNTSRVPKPWLEVHNPTSLPAGLPGRGL
                     SLSGRTERSWQIRSLLTRRGHFRIEPLQIRTGDPFGFFESSASVGSGINVTVYPRLEP
                     LPLWRLPASNLDGSHAMRERTLQATPLATTVRPWAPGDAFNRIHWKSTARHGDIQVKE
                     FDLEQTADAWIVLDLERAAQRGTGDESTVEVAVRVAAAVADKALVENRAVGMTVSAHR
                     LGQLQPDRGGRQHLKVMQLLAAVEGDGAIPLTEALVASVQRIRRGMTAVIITASLDRD
                     WVKPLAALRSRGVACVVVALDVAAFERHARETAAREQQEPVGFVSVLVRPEDEAPTRA
                     APAAATSSATSAEVPESEIASAKHARALRHALAEYEIAVYQVGPTSPLAKALAS"
     gene            7970..>10047
                     /locus_tag="VIF63_02420"
     CDS             7970..>10047
                     /locus_tag="VIF63_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY8238265.1"
                     /translation="MSAATVTAPDRRFAAFRNLPFAPVEGWLTLLVTGAMVVVLGWSL
                     QEASWTQGPTEDGGFLPWVGFVGLAFGVGGAKVGWGRWRTHLVGAAFAGLVLPLIVGG
                     IVLHTSAWDPNTLAHRYVASLDVIRRVWNDLAVLGLSFTNESAHYHLVFGAIVWAAGM
                     LAGFDVFGHRRPLDAIVVLGLLLLANLGLTSHQQLPFLVAFSFAALLLLIRTHVFEEE
                     VVWTRRKVGDPSSVRQLYLIAGAQFVIVAVLGSVLLTWTASSAPLQGLWRDLPQQLAS
                     ITQWLQRYAPGGGDFAGPGLVSFLPNAPTIGQWQPSNVISFRAQLSPTEEQLFKWRAG
                     TYAEYQGNGWQWGPTRDEPTEPGATLLGRDGSGDHPVETGRRQISARVMPEGFIGRTI
                     IGPNTLLSVDRPSTAKVTGANGWYTTIATDNDPGTYNITALIPILEGDGALTEAELRL
                     AGTNYPSEIREIYLALPKDAVGPFSTALLKDIRAAVVAPGYADPANAYDLARTMETYL
                     RDDRHFTYDEDVRDEKFAQCGNASSVECFAIIRRGYCDYYASTMAVLLRSAGVPARIA
                     YGFLPTPRNADGLEIVPGSSAHYWVEVYFPDIGWVEFDPTGGSIGSPVVLPSGSPPLV
                     TPRPSGLPTSTPFDITAPPTNGPSAPPVTPPGTGIGPFIAIAFILMIVVIGAVYAAYR
                     RTPTGPMHPD"
BASE COUNT         1349 a         3705 c         3442 g         1551 t
ORIGIN      
        1 tcggcttcgg caatggctcg cgcctcggcc gctgcacgct cgcgcgctgc ttcctcggcc
       61 gccgcccggg cgtcggcctc ctggatcgaa gcgatcgcgg ccgcttctgc ctcggcacgc
      121 ctcgcggccg cttcggcttc cgcggcggcg cgcgcctccg cctctgcgat ctcacgggcc
      181 tcggtttcgg cggcgatccg ggcagcttcg tcggcctcgg tctgcgcggc cgcctcggcc
      241 tcggcctgcg caactgcagc tgcttccgcc tcggcctggg cggccgctgc cgcatccgcc
      301 gcagcctgcg ccgccgcggc cgcctccgac gccgccacgg cagctgcttc ggcctcggca
      361 aacgcggccg attcggcgtc cgcggcggcc tgggcctccg ccgcggcctg ggcctcggcc
      421 tcagcgacgg cggcgtgatc gatgatcggc tcggcgacga tgatggcggg cgcccgggcg
      481 aggtccatcg gctggagcga gacggccgtg tcgacgcggg tctcgggcgg cggggcatcc
      541 ggcgtcgggg cgcagctcag gcagcgaccc tcgacgacgt tccagcaatc gccgcaggtg
      601 tattgccggc acgacaggca gaactggaac gtgcgatgga aggcctcgag tgcggtcgcg
      661 gtggagcgct gctccgtctc ggagcgagcg acggcaaagg cctcatcgag cgatgagccg
      721 ggatcgctca cgaaatggcg gatgccgcga ccgagcgtgc cgatgcgcga caggggcttg
      781 cgcctgctgc tcaccgtttc gaacgtgtag cgcgtgccac accgctcgca gaaggattcg
      841 gtcaggatct cggtcattgg ctcccgtgat gataccggcc gcccgcattc gctccgtgat
      901 gacgctggta cccccgtcgt gggacagggg taccgttggt tacggaggag ggctgaacgg
      961 aggcctctgg taggctctcg cccgtgcgag ccatagcccg cgtggagcgc ttcatcgagc
     1021 gcctcgtcga gcgcccgagc gcccgcctct tccgaacgag gctccagccg gtccaggtcc
     1081 tgcgccacat cgagcgcgtg atggaagcgg gacgcggcgc cgagggcacg cgcggcctcg
     1141 tccccgacca gttcgtcgtg aagctccacc ccgaggacct cgccgcattc ctgcccgccg
     1201 acgagctcgc cagcgagctc gcctcggggg cgttgcgctt cgcccgcgcc catggctacg
     1261 cgctccgcga tcgcccgcga gtgaccctcc agccgaaccc cgacctccga ccggccgagg
     1321 tcgatgtcga ggccgaggtc tcacccctgg ccggacaggc cgatggcggc gagcgggtcg
     1381 acggcgggac ccgggtcttc gaggtgcccg tggtgcgtgc gccgcaggtg gtcatcgagg
     1441 tgcgcgagcc gaaccggaag ccccgacgcg ttccgatgac cggcgcgccg gtccacatcg
     1501 gccgcgcgcc ggagtgccag ctcgtgctca aggactcgcg cgtgtcccgg cggcatgccc
     1561 gcttgcacgc tcgcgacggc gtcctcgtcc tgaccgacct cggctcgacg aacggcacac
     1621 gtgtcaacgg ccatcgcgtc accgagctcg tgctcggcgc cggcgatcgc atccagatcg
     1681 gcgagacgag cctcgtcgtc gaagccgcgc cgtccgaagc gcctgtcggg acgatcgccc
     1741 ggccatagcc gggcgctgcc atggacgccg ccgtcctcgc gatctgggtc ctgcgcctcc
     1801 tgtttctcgg cctgctctat ttcttcctgt ggtcggtcgt gcgcgcgctc ctgcgtgacc
     1861 tgcgggcggc ggcccgcgag ccgacgcgcg agctcggccg cctcgtcgtg gtcgcgtcgc
     1921 ccggcggcga gcccgaggcg ggcgccgtct tcgccctcga tgcgatcacg tcgctcggtc
     1981 gggacgtcaa taactcgatc gtgctcgacg acgacttcgt ctccgccgcc catgccgcgc
     2041 tgacctatcg gggcagggcc tggtacgtcg aggacctcgg ctcgacgaat ggcaccttcg
     2101 tcaacggcag ccaggtcgac ggcctgtcgc cgatcgcgtt cggggatgag atccagatcg
     2161 gccaggtccg catgcggctc gagcgcgctc gggcgtgacc agcgcgggcg tgaccagcgc
     2221 gggcgtgacc acggcgcggc gcttgcgcac cgtcgggcgc gggggccggc ggttccgcat
     2281 cacgccccgg ctgcgcggtc gcgagctcgg gttgctcctc ctgtgcgggg ttgcgctgtt
     2341 cgtcggcagc tggtcgctcg gcgccacgca ggccttccgt gccgcccagc tcgacggcga
     2401 caccgggtcc ctgaacctat cggaagccgc agagcccggc ttgctgacca tctatctgct
     2461 ggccctgttc ggggtccacg ccgcgttggt cctcgccggg cggcgcacgg accaggtcct
     2521 gctcccggcc gtgggcatgc tcggcgggat cgggctgctg ctcatgatcc ggctgcccca
     2581 ggacctcgcg ggcaggctgg gcggactcgc ccagacgcag ctggtctggc tcgtcatcgg
     2641 gctcgtgatc atcgcgggcc tgggcgtcgc ggtccggaac gatgtctggc tccggcgcta
     2701 caagtacacg tgggcggcgg tcggggtcgg cctgctgctg ctcgtgttcc tgttcgggcg
     2761 ggacatcaac ggcgcacgcc tgtcgctcac gatcgggccg ttcagcggcc agccctcgga
     2821 gctgctcaag gtcatcctcg tcgtgttcct ggccggctat ctctcggaga atcgcggcct
     2881 cctcgtcgag gagtccacgc ggatcgggcc gctccggctg ccaccgctgc cgtacctcgc
     2941 gccgatggtc gcaatgtggg cgatcgccct gggcatcgtg atcgtccagc gcgacctggg
     3001 cgcggcgctc ctgttcttcg cggtcttcct gctgctcctg tacgtctcga ccgcgcgcgt
     3061 gagcttcgtc gtgctgggcc tcgcggcgtt cgtcgtgggc gcgggcgcgc tcgcgctcgt
     3121 cttcgaccac gtgcgcacgc gcatcgacat ctggctcgac ccgttcgcag atccatcggg
     3181 cgccggctac caggtcgtgc aggcgctgca cgcatttgcg cgcggtgggc tgatcggcac
     3241 gggcctcggc ggcgggttgc ccacgatcgc cggccggccg ccgattccgg ccctccacac
     3301 ggacttcccg ttcgccgcgc tcggcgagga gctgggcctc gtcgggatcc tcgcgatcct
     3361 cgggctgtac ctcgtgctgg tcgagcgcgg cctgcggatc gcctccgcgg cgcaggacga
     3421 gttccgcgcg atcctcgccg ccggcctctc gctcgtcatc ggcgtccagg cgttcatcat
     3481 cgccggcggg aacctcaagc tgatcccgct gaccggcatc acgctgccgt tcgtgagcta
     3541 cggcggctcg tcgctgctcg cgaactcggt gatcgtgggc ctgctgatcg ccctctcgga
     3601 acggacgcgc gagcccctcc cgccaccacg ccgggccggc aggctccggc ggatgctcga
     3661 tcgaggcgcc gcctgatgcc aggcgagccg cagcccgagc gggcgccggc accgcgcgag
     3721 cgcgcccctg cctcgcgcaa gcccgtccac gcctacccga tctcgggctc cgcgccgtcg
     3781 tggacgggcc gccgcgtcgg catcggcgcc gcgctcaacc gggtggccct cgccctcgcc
     3841 gtcgcgttcg gcgggctcgc cctcggcgcc ggctactggc aggtcatcga gtcgtccaac
     3901 ctcagcacgg caggcgacga tgccgccgtt atcgcggcgg cacgccacgt caagcgcggc
     3961 gagatcttcg atcgagacgg cgttcgcctc gcctggaaca aggtcgacaa gaacggcgag
     4021 ccgtatcggg tgtatgcctc gaacacgctg tccggcgtca tcggctactc gtcgcgccag
     4081 tacggcaccg cgggcctcga gagcgcctgg aacgccgagc tcagcggggc ggtctccgcc
     4141 gatccgctgc gcgagctgac ccgcaagttc cgtgccgacc catccgatcc ccaggacatc
     4201 aagaccacgc tcgtgctcga gctccagaag gcggcggtgg cggcgctggg ctcgaagaag
     4261 ggcgccgtcg tgctcctcga cccgcgcacc ggcgaggtgc tggcgctggt ctccaccccc
     4321 accttcgacg catcggacgt cgccaacccg gccacgtcga cgagggcctt cgacgcgctc
     4381 cggacggacg accgattgcc cctgctgccc cgggccacga gcggcctgta cgtgcccggc
     4441 tcggtgttca agatcgtgac cgcggtcgcg gccctcggca gtggggccgt gacgccggac
     4501 acgagatacg aagaccagcc cggctcggag aagaagggct ggctggtcga tggcttccgc
     4561 gtgcaggacg gccaccacac cgcgaccggc agcaaggcgc tcaacttcgc cgaggccgtc
     4621 gaggtgtcgt gcaacatctg gttcgccgag accggcgttc gcacgggcgg cgaatcgctc
     4681 gcggtctggg cccagcgcct cggcttcggc gccccgatcc cgttcgacct gccgacgaag
     4741 gccagccagg tcaccaacgg cggcggctcg ttcggcggcg gcttcgccga tcgaatcgag
     4801 ctcgccaacg ccgcgtacgg tcagggcgag acgctggtga ccccgctgca gatggccctc
     4861 gtcgcggcga cgatcgccaa cgacggcgtg ctcatgcgcc cgcacctgat gctcgaggcg
     4921 accggcaagt ccgggacgac caccgtcaac ccgagcacgc tcgagcgcgt catcgcgccg
     4981 ggcgtggccg aggagatcgg ggcggcgatg cgcctcgcgg tgagcggtca gctcgggcgg
     5041 gtgtacacct cgggcgcctc ggtgcgcgga ctcgcggttg ccggcaagtc gggcaccgcg
     5101 gagctcgacc cgggccgcgc gccccattcg tggttcatcg gcttcgcccc gtacgacgat
     5161 ccccaggttg cgatcgccgt cctcgtcgag ggctcgggca gcggcggggt ccgggcgtca
     5221 ccgctggcgg gccagatcct ccgagcctgg cgcgactggg cgaacccgtg agcaatgaga
     5281 cggcgccgca gccggagtcc aagccgcggc tggaggcccc gcctcggccg atgatcgagc
     5341 ggctcggcct cgcgctgatc gcgctgctgc tcgctggaat gttcggcgcg ctcggctacg
     5401 tgtcactgac cggcggtgag gtcttcctcg ggatcatggg cgtgatcggg gcgctcatga
     5461 cggtgtgggc ggccgccagc tcgctacggc gcggctgatc gggcaaggaa cgtgccgaga
     5521 acccaacggc caatcgcgtc gtaccatccc gtgacactcg gccgggcgta atggggagcc
     5581 acatgagcag gatccaggag accggggacc gcgtcctcgc caacgtcgaa cgcgtgatcg
     5641 tcggcaagca caacgaggtg cgcttcgccc tcgtcgcgtt gctgtgcaag ggccacctgc
     5701 tgatcgagga cgtcccgggc accggcaaga ccgtcctcgc caaggcgatc gcgaagagcc
     5761 tcggctcgtc gttccggcgg atccagttca cgcccgacct gctcccgtcc gacgtgaccg
     5821 gcctgtcgat ctacaaccag aagaccacgg agttcgagtt ccggcccggc ccgatcatgg
     5881 cccaggtggt gctcgccgac gagatcaacc gcgccacccc gaagacgcaa tccgccctgc
     5941 tcgaatgcat ggaggagcgc caggcgacgg tcgacgggat cacccacatg atgcccgacc
     6001 cgttcctcgt catcgccacc cagaacccga tcgagtacga gggcacgttc gcactgcccg
     6061 aggcgcagct cgacaggttc atgctccgga tccgcctcgg ctacccgaag ccggtcgagg
     6121 aggtcgtgat cctcgacgag cagaagcgcg agcacccgat cgatgacctc gaggtcgtgt
     6181 gcagcgtcga ggagctccag gagatgcagc gcgcggtgcg cgagatctac gtcgacccga
     6241 ccgtttccga gtacatcgtc cggctggtca acggcacgcg cgaccacggc gacatctacc
     6301 tcggcgcgtc gccccgcggc tcaatcgccc tgtatcgcgc cggccaggcg ctcgccggcc
     6361 tgctcggccg cgactacgtg atccccgatg acgtcaaggc cctcgccgaa gccgcgctcg
     6421 cgcaccggat gatcatcaag acgagctcgt cgatccacga cgtccagccc gcccaggtga
     6481 tccgcgagct cctcgaatcg acctcagtcg gggccgtgaa ggagccggcg gtggccggcc
     6541 tcagtgaggc caaggccggc gtgcgcacgc gctcggacca gggctagcaa gggtcgcagg
     6601 gaaaggctac cgagccggac gagcaggcac cgatgttccg tcgactccag ctcctgatcc
     6661 tcgccgggat cctcgtgatc gcggcgttct cgagcggcaa ggacttcctc ttctacctcg
     6721 tctatctcgg cgtgctcgtc atcggcggct cgtacgtcct gacgcggctc ggcttggccg
     6781 acctcgaggc gggctacgcg gtcagccagc tgtcgggcca cgtcggcgac cgcatccagg
     6841 tcacgtacac gctgcgcaac acgagccgcg tcccgaagcc ctggctggag gtccacaacc
     6901 cgacgtcgct gccggccggc ctgcccggcc gcggcctgtc gctcagcggt cgcaccgagc
     6961 gctcgtggca gatccgcagc ctcctcaccc ggcgcggcca cttccggatc gagccgctcc
     7021 agatccggac gggcgacccg ttcgggttct tcgagtcgtc cgcgtccgtt ggctcgggca
     7081 tcaacgtcac cgtctatccg cggctcgagc cgctgccgct ctggaggctg cccgcctcga
     7141 acctcgatgg cagccacgcg atgcgcgagc gaacgctcca ggcgacgccg ctcgcgacga
     7201 ccgttcgacc gtgggcgccg ggcgacgcgt tcaaccgaat ccattggaag tcgactgcac
     7261 gccatggcga catccaggtg aaggagttcg acctcgagca gaccgccgac gcctggatcg
     7321 tgctcgacct cgagcgcgcc gcgcagcgcg ggaccggtga cgaatccacg gtcgaggtcg
     7381 cggtccgggt cgccgctgcg gtcgccgaca aggcgctggt cgagaaccgc gccgtgggca
     7441 tgaccgtgag cgcccaccgg ctcggccagc tccagcccga tcgcggtggt cgacagcatc
     7501 tcaaggtcat gcagctgctc gccgccgtcg agggcgacgg cgcgatcccc ctgaccgagg
     7561 cgctcgtggc cagcgtccag cgcatccgcc gtgggatgac cgcggtgatc atcaccgcct
     7621 cgctcgaccg cgactgggtc aagcccctcg ccgcgctgcg ctcgcgaggt gtcgcctgcg
     7681 tcgtggtcgc gctcgacgtc gcggcgttcg agcggcacgc tcgcgagacg gcggcacgcg
     7741 agcagcagga acccgtgggc ttcgtctccg tcctcgtccg gcccgaggac gaggcgccga
     7801 cgcgtgcggc gcccgccgcc gcaacgtcga gcgccacctc cgccgaggtc cccgaatccg
     7861 agatcgccag cgcgaagcac gccagagccc tgcgccacgc cctcgccgaa tacgagatcg
     7921 ccgtctacca ggtcggcccg acgtcgccgc tcgccaaggc cctcgcgtcg tgagcgccgc
     7981 caccgtgacc gcgccggacc gccggttcgc ggccttccgg aacctgccat tcgcgccggt
     8041 cgagggctgg ctgaccttgc tcgtgacggg cgcgatggtc gtcgtgctcg gctggtcgct
     8101 ccaggaggcc agctggacac aggggccgac agaggatggc ggtttcctgc cgtgggtcgg
     8161 gttcgtcggg ctggccttcg gcgtcggtgg cgccaaggtt ggctggggtc gctggcgcac
     8221 gcatctcgtc ggcgccgcct tcgccgggct cgtgctgcca ctcatcgtcg gtgggatcgt
     8281 gctgcacacg tcggcgtggg acccgaacac gctcgcgcac cggtacgtgg cgtcgctcga
     8341 cgtcattcgt cgggtctgga acgaccttgc ggtgctcggc ctctcgttca ccaacgagtc
     8401 ggcgcactac cacctcgtct ttggggccat cgtgtgggcg gcgggcatgc tcgccggctt
     8461 cgacgtcttc gggcaccgcc ggccgctcga tgccatcgtc gtcctcgggc tcctgctgct
     8521 tgccaacctg ggcctcacga gccaccagca gctgcccttc ctggtcgcct tcagcttcgc
     8581 ggcgctgttg ctgctcattc gaacccacgt gttcgaggag gaggtggtct ggacgcgacg
     8641 gaaggtcggt gacccgtcgt cggtgcgcca gctgtacctc atcgccggcg cacagttcgt
     8701 catcgtggcc gtgctcggct cggtcctgct gacgtggacg gcctcgtccg cgccgctcca
     8761 gggcctgtgg cgcgacctgc cccagcagct ggcatcgatc acgcaatggc tgcagcgcta
     8821 cgcgccgggc ggcggcgact tcgccgggcc gggcctcgta tcgttcctgc cgaacgcgcc
     8881 gaccatcggg cagtggcagc ccagcaacgt gatttctttc cgcgcgcagc tctcgccgac
     8941 cgaggagcag ctgttcaagt ggcgggcagg cacgtacgcc gagtaccagg gcaacgggtg
     9001 gcagtggggc cccacccgcg acgagccgac cgagcccggg gccaccctgc tcggccggga
     9061 cggttcgggc gaccatcccg tcgagaccgg ccggcgccag atctcggccc gcgtcatgcc
     9121 cgaggggttc atcggcagga ccatcatcgg cccgaacacc ctgctcagcg tcgaccgtcc
     9181 gtcgacggcg aaggtcaccg gtgccaacgg ctggtacacg acgatcgcca cggacaatga
     9241 cccgggcacg tacaacatca ccgcgctcat cccgatcctc gaaggcgatg gcgcgctcac
     9301 ggaggccgag ctccggctcg cgggcaccaa ctacccctcc gagatcaggg agatctacct
     9361 cgccctgccc aaggacgcag tgggcccctt ctcgacggca ctcctcaagg acattcgcgc
     9421 cgcggtcgtc gcgccgggct acgccgaccc ggccaacgcg tatgacctcg cccgaaccat
     9481 ggagacctac ctccgggacg accggcattt cacgtacgac gaggacgtcc gcgacgagaa
     9541 gttcgcgcaa tgtggcaatg catcgagcgt cgagtgcttc gcgatcatcc gccgtggcta
     9601 ctgcgactac tacgcgagca ccatggccgt gctgctgcgc agcgctggcg tccccgcgcg
     9661 catcgcctac ggcttcctgc cgaccccgcg caacgccgac ggtctcgaga tcgtgcccgg
     9721 cagctccgcc cactattggg tcgaggtgta cttccccgac atcggctggg tcgagttcga
     9781 tcccacgggc ggcagcatcg gcagtccggt ggtgctccca tccgggtcgc cgcccctggt
     9841 gactccgcgg ccgagcggcc tgcccacctc gacgccgttc gacatcacgg cgccgccgac
     9901 caacgggccc agcgcgccgc cggttacgcc gcccggtacc ggcatcgggc cgttcatcgc
     9961 gatcgcgttc atcctcatga tcgtggtgat cggcgccgtg tacgcggcgt accgccgaac
    10021 cccgaccggg ccgatgcacc cggacca
//
LOCUS       DATBSW010000089         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_676819, whole genome shotgun sequence.
ACCESSION   DATBSW010000089 DATBSW010000000
VERSION     DATBSW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676819"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VIF63_02425"
     CDS             complement(<1..744)
                     /locus_tag="VIF63_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238266.1"
                     /translation="MTFTLEPRVSASRWLSPAATIGAVLIALVAGGVMIAFAGGDPIR
                     SYVHILNAAFGSVGVISDTLVKATPLILTGLACAIAFRMRLWNIGAEGQFLMGAWGAS
                     AVVLAPLLPAGTPGIVLIPAMLLAGAVAGALWALVPGILRARLGVNEIITSLMLNYVA
                     LSWVQFWVFGPWSEGGFQQTRTFPAEAWLPRLADLAVDFPALSGLTVHVGLIFGLVAA
                     AVLWLLLERGKWGYEISLVGDSPPAARYAG"
     gene            complement(741..2276)
                     /locus_tag="VIF63_02430"
     CDS             complement(741..2276)
                     /locus_tag="VIF63_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238267.1"
                     /translation="MTAAPDPQPQAAIRASGIVKRFPGVLANDHVDFDLRAGEVHALL
                     GENGAGKSTLMNILAGLYSPDEGEIEVHGQPVSFRSPRDAIASGIGMVHQHFALVPSM
                     TVTENVILGLDRPRFRLGLARFDDEIRKIAESVGLHVDPRARIWQLSVGEQQRVEILK
                     LLYRGARILILDEPTAVLAPQEIDELFATLRSMTKAGRSVVFISHKLGEVLAIADRIT
                     VMRRGKVTAADVPAAGATKEELARLMVGRAVLERIRRIEREPGEVVLEVTNVHADNDR
                     LLPALRGASLTIRSGEIVGLAAVAGNGQAELAEVVTGLRACRGSIRMNGEEIANRSPG
                     HAIRRGIGHVPEDRTAVGSAPNLSLTDNAMMKRYRDVPIARGWLLHRPAARQLAEQLT
                     TEYSISVPSVESEARLLSGGNLQRLILAREIERKPGLLVAVQPTRGLDVGAIETVHQL
                     LLDRRAEGAAILLISEELDELLALADRIDVMYEGRIVGSFPPDAELIHDIGLRMTGGA
                     AAA"
     gene            complement(2349..3596)
                     /locus_tag="VIF63_02435"
     CDS             complement(2349..3596)
                     /locus_tag="VIF63_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8238268.1"
                     /translation="MSRRLRFLSTLAVGLLVVTACQATPGGSAAPSGAASGCTAAAGT
                     GVQIPEIEAGKFNVAMVLIGPHDDAGWSQAHFEGLQYVCEHVANAHVAYIENVLDTAD
                     SEQVFRSLSRKGFDFIIGTSFGYMDPMDAVAKEFPAITYLHLTGFKSNGTNFGNFFGA
                     MEDFKYLAGMLAGSRAKKDGVTKIGYMATFPIPEELRLGNAIMLGVKKTCPECTMDIR
                     FINTWHDPVKEKDGAASLFDAGAQVVFTGADTGAVADVAQEKGKWAVTYDHQASCKVD
                     ACLTAPYWVWGPEYVRIAEQVKAKTYKAGYEYFDANTKAMGLMGFMEGQSPRKGVADL
                     PEADLKLVRDTLAQMLAGTFGRFDVFAGPIKDNTGKEIVAAGAKLEQADLDQFPPGAG
                     DGCKTCMYWWADGITSELPSLTP"
     gene            complement(3748..4182)
                     /locus_tag="VIF63_02440"
     CDS             complement(3748..4182)
                     /locus_tag="VIF63_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238269.1"
                     /translation="MPSQPPAKFFVNQGVVTSREDMLNLLRPLLKQTVSYRYLRGREV
                     AAHGTGWVERIVVDQPEVSTYFTPIAITLNVDSFEHLDFQTRPDQLLVYTLVQGDESV
                     VVEFTPGSDGSGNPFEPHQLAFDTSGYVQMELQGLDSAPAEE"
     gene            4286..5098
                     /locus_tag="VIF63_02445"
     CDS             4286..5098
                     /locus_tag="VIF63_02445"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8238270.1"
                     /translation="MLLAVDVGNSNVTIGSFRNGSLVAVRRAATPRGGTADELELLVE
                     GLLRLDDAAFADVSAVVAASVVPAVSAALESVTARRDRPLLLAGAGTVPLPIRVDRPG
                     EVGADRLVNAFAASRLYGTPAIVIDLGTATTFDCVARDGAFVGGAIAPGIELGLEGLA
                     SRTAKLPMVEIRTPDRAIGRDTVSAIQSGAVLGYQELVSGLLGRIRRELADASDVAPA
                     DVHVILTGGHSQAPWAGALEGVEAIDVDLTLKGLAILHAEVAGGQALELPLT"
     gene            5095..>5491
                     /locus_tag="VIF63_02450"
     CDS             5095..>5491
                     /locus_tag="VIF63_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY8238271.1"
                     /translation="MTAATAKRLDGRLIALGVTGSIAAYKAVELLRLLTAEGADVVVL
                     LSPSATRFVGPLSFAALSRHPVESDVLDLLPDGRIGHIVIADSADALVVAPATAHWLA
                     AMANGLAGDVVTAAALASSAPVVVAPAMDG"
BASE COUNT          809 a         1897 c         1938 g          847 t
ORIGIN      
        1 ccccgcgtac cgggcggcgg gcgggctgtc gccgacgagg ctgatctcgt agccccactt
       61 gccgcgctcc agcaggagcc acaggaccgc cgccgcgacc aatccgaaga tgaggccaac
      121 gtggacggtg aggcccgaca acgccgggaa gtcgacggcg aggtcggcga ggcgaggcag
      181 ccaggcttcc gccgggaacg ttcgcgtctg ctggaagccg ccctcgctcc acggcccgaa
      241 gacccagaac tggacccagg acagcgcgac gtagttgagc atcagcgagg tgatgatctc
      301 gttcaccccg agccgagccc ggaggatgcc cggcacgagg gcccacaggg cgcccgccac
      361 ggcaccggcg agcagcatcg ccgggatgag cacgatcccg ggcgtgccgg ccggcaggag
      421 cggggcgagc acgacggcac tcgcgcccca ggcgcccatg aggaactgcc cctcggcgcc
      481 gatgttccag aggcgcatcc ggaacgcgat cgcgcacgcc agccccgtca ggatcagcgg
      541 cgtcgccttc accagcgtgt ccgagatgac cccgacgctg ccgaacgccg cgttgaggat
      601 gtggacgtag gaccgaatcg gatcgccgcc cgcgaacgcg atcatcacgc cgccggcgac
      661 cagggcgatc aggactgcgc cgatcgtggc ggccggcgac agccagcgag atgccgagac
      721 gcgaggctcc agcgtgaagg tcatgcggcc gcggcgccgc cggtcatccg caggccgatg
      781 tcgtggatca gctcggcgtc cggcgggaac gagccgacga tcctgccttc atacatgacg
      841 tcgatccggt cggccagggc gaggagctcg tcgagctcct ccgagatgag caggatcgcc
      901 gctccctccg cgcggcggtc gaggagcagc tgatggaccg tctcgatcgc cccgacgtcg
      961 aggccgcggg tgggctgcac ggcgacgagg agccccggct tgcgctcgat ctcgcgcgcg
     1021 aggatcaatc gctggaggtt gccgcccgag agcaggcgcg cctccgactc gaccgacggg
     1081 accgagatcg agtactcggt cgtcagctgc tccgccagtt ggcgcgcggc ggggcgatgg
     1141 agcagccagc cccgcgcgat cgggacgtcg cggtagcgct tcatcatcgc gttgtcggtc
     1201 agcgagagat tcggggcgga gccgaccgcc gtcctgtctt cggggacgtg accgatgcca
     1261 cggcggatgg cgtgtcccgg cgagcggttg gcgatctcct cgccgttcat tcgaatgctc
     1321 ccgcggcacg cgcgcagacc ggtcacgacc tcggcgagct ccgcctgccc gttcccggcg
     1381 acggcggcga gcccgacgat ctcgccggac cggatcgtga gcgaggcgcc gcgcagcgcg
     1441 ggcaggaggc ggtcgttgtc ggcgtggaca ttggtcacct cgaggacgac ctcgcccggc
     1501 tcgcgctcga tccgccgaat gcgctcgagg accgcccggc cgaccatcag tcgcgccagc
     1561 tcctccttgg tcgcgcccgc agcgggcacg tccgcagccg tgacctttcc gcgccgcatg
     1621 accgtgatcc ggtcggcgat cgcgaggacc tcgccgagct tgtggctgat gaacacgacg
     1681 ctgcgacccg ctttcgtcat cgatcggagc gtcgcgaaga gctcgtcgat ctcctgcggg
     1741 gcgagcacgg cggtcggctc gtccaggatg aggatccggg ccccgcgata cagcagtttc
     1801 aggatctcga cccgctgctg ctccccgacg gacagctgcc agatgcgggc gcgcggatcg
     1861 acatgcagcc cgaccgactc ggcgatcttg cggatctcgt catcgaagcg cgccaggccg
     1921 aggcggaagc gtggccgatc gagcccgagg atgacgttct cggtgacggt catcgacggc
     1981 accagcgcga agtgctggtg gaccatcccg atccccgacg cgatcgcgtc acgtggcgag
     2041 cggaagctga ccggttggcc gtggacctcg atctcgccct cgtccgggct gtacagcccc
     2101 gcgaggatgt tcatcagggt gctcttgccg gcaccgttct cgccgagcag cgcgtggacc
     2161 tcgccggcgc gcaggtcgaa atcgacgtga tcattcgcga ggacgcccgg gaatcgcttg
     2221 acgatccccg aggcgcggat ggccgcctgc ggttgcgggt caggagcggc ggtcatccgg
     2281 cctccggaaa cgaatcgagg ccgcggacag gatgcccgcg gcctcgagac atcgctagct
     2341 agctgggact acggcgtgag cgaggggagc tcgctcgtga tgccgtccgc ccaccagtac
     2401 atgcaggtct tgcagccgtc gcccgctccc ggagggaact ggtcgaggtc tgcctgctcg
     2461 agcttcgcgc ctgcggcgac gatctccttg ccggtgttgt ccttgatcgg cccggcgaag
     2521 acgtcgaacc gtccgaacgt gccggcgagc atctgggcca gggtgtcccg gaccagcttc
     2581 aggtcggctt cgggcaggtc ggcgacgccc ttgcgcggcg actggccttc catgaagccc
     2641 atgaggccca tggccttcgt gttggcgtcg aagtattcgt agccggcctt gtaggtcttg
     2701 gccttgacct gctccgcgat ccgtacgtac tccggacccc agacccaata cggcgccgtc
     2761 aggcaggcat ccaccttgca cgaagcctgg tggtcgtacg tgaccgccca cttgcccttc
     2821 tcctgcgcaa cgtccgccac cgcgccggta tcggcgccgg tgaagacgac ctgggcgccg
     2881 gcgtcgaaga gggaggccgc gccgtccttc tccttcaccg ggtcatgcca ggtgttgatg
     2941 aaccggatgt ccatcgtgca ctccgggcac gtcttcttga cgccgagcat gatcgcgttg
     3001 ccgagccgga gctcctccgg gatcgggaac gtggccatgt agccgatctt ggtgaccccg
     3061 tccttcttcg cccgcgagcc cgcgagcatg ccggcgaggt acttgaagtc ctccatggcg
     3121 ccgaagaagt tgccgaagtt cgtgccgttc gatttgaagc cggtcaggtg gaggtaggtg
     3181 atggccggga attccttggc cacggcatcc atggggtcca tgtagccgaa ggacgtcccg
     3241 atgatgaagt cgaagccctt gcgcgaaagg ctccggaaca cctgctcgga atcggcggtg
     3301 tcgaggacgt tctcgatgta cgcaacgtgg gcgttggcga cgtgctcgca gacgtactgg
     3361 aggccctcga agtgggcctg gctccagccc gcgtcgtcat gtggcccgat gaggaccatc
     3421 gcgacgttga acttgccggc ctcgatctcc gggatctgga cgccggtgcc cgcagctgcc
     3481 gtacagccac tcgcggcgcc gctcggcgcg gctgacccgc ccggtgtcgc ctggcacgcc
     3541 gtgaccacga gcaatcccac cgcgagggtg ctcaggaacc gtaaccgccg actcaccatc
     3601 gatctgctcc tcctcgacgc ctggacctgg ggactgtgcg cgctgctgcc gtgatgcagg
     3661 tggcccgacg ggccggcgcg accgttgact ccgaaccggg acaggataga tgcgccgggg
     3721 cggcccgggc gccccaaact cgccgcgcta ctcctccgcg ggcgccgaat cgaggccctg
     3781 cagctccatc tggacgtagc ccgaggtatc gaatgcgagc tggtgcggct cgaacgggtt
     3841 gcccgagccg tcgctgcccg gcgtgaactc gacgacgacc gattcgtcgc cctggacgag
     3901 ggtgtagacg agcagctggt cggggcgcgt ctggaagtcg agatgctcga acgaatcgac
     3961 gttgagcgtg atggcgatcg gggtgaagta cgtcgagacc tcgggctggt cgacgacgat
     4021 ccgctcgacc cagccggtgc cgtgcgccgc gacctcgcgc ccgcgcaggt agcggtagga
     4081 cacggtctgc ttgagcagcg gccgcaggag gttgagcatg tcctcgcggc tcgtgaccac
     4141 gccctggttc acgaagaact ttgccggcgg ctggctgggc acgagctcct cctccggggc
     4201 acgtcgggtc gggagggaac gcggccgatg gtagcgcggc acgctgccca ccgggacccc
     4261 gaaatcggcc gcgtatgatc cggccatgct ccttgcggtc gatgtcggca actcgaatgt
     4321 cacgatcggc tcgttccgga atggatcgct ggtcgcggtc cgccgggcag cgacgccgcg
     4381 cggtggcacg gccgatgagc tcgagctgct ggtcgagggc ctgctccgcc tcgacgacgc
     4441 cgcgttcgcg gatgtctcgg cggtcgtcgc cgcgtccgtc gtgcctgcgg tcagcgcagc
     4501 gctggagtcc gtcaccgcgc gtcgcgaccg acccctgctc ctcgccggcg ccggcacggt
     4561 gcccctgccg attcgcgtgg accggccggg cgaggtgggc gcggatcggc tcgtgaacgc
     4621 cttcgcggca tcgcgcctct acggcacgcc ggccatcgtg atcgatctcg gcacggcaac
     4681 gaccttcgac tgtgtcgccc gcgacggggc gttcgtcggt ggtgccatcg cgccgggcat
     4741 cgagctcggg ctcgaagggc tggcctcgcg caccgcgaag ctgccgatgg tcgagatccg
     4801 gacgccggac cgagcgatcg ggcgcgacac ggtctcggcg atccagagcg gggccgtgct
     4861 cggctaccag gaactcgtct cggggctgct cggccggatc cggcgagagc tcgccgacgc
     4921 cagcgacgtc gcacccgccg acgtccacgt gatcttgacc ggcggccact cgcaggcgcc
     4981 gtgggccggc gcgctcgagg gcgtcgaggc gatcgacgtc gacctgaccc tgaaggggct
     5041 cgcgatcctg cacgccgagg tcgcgggagg ccaggcgctc gagctgccgc tcacgtgact
     5101 gccgccaccg ccaagcgact ggatgggcgg ctgatcgcgc ttggtgtcac gggatcaatc
     5161 gcggcgtaca aggccgtcga gctgttacgg ctcctcaccg ccgagggcgc cgatgtcgtc
     5221 gtgctcctgt cgccgagcgc gacgcggttt gtcgggccgc tgtcgttcgc tgccctgtcg
     5281 cggcaccccg tggagtcgga cgtcctcgac ctcctgcccg acgggcgcat cggtcacatc
     5341 gtcatcgcgg attccgctga tgccctggtt gtcgcgcccg cgaccgcgca ctggctcgcg
     5401 gcgatggcca acggcctcgc cggcgatgtc gtgaccgcgg ctgcgctcgc ctccagcgcc
     5461 ccggtcgtgg tcgcgcccgc gatggacggc g
//
LOCUS       DATBSW010000090        12013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_289074, whole genome shotgun sequence.
ACCESSION   DATBSW010000090 DATBSW010000000
VERSION     DATBSW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289074"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1269)
                     /locus_tag="VIF63_02455"
     CDS             complement(133..1269)
                     /locus_tag="VIF63_02455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8238272.1"
                     /translation="MRIAILGTRGVPASYGGFETFAEELGARLVQRGHEVTVYGRSHV
                     VPKGITTHRGIRIRRLPTIRHKYLDTVLHGAISVLDALPRRYDVVLICNNANAPFALV
                     PRLGGAKVVLNTDGLEWERGKWNSLGRWYAQACAWLAPKLPIVLIADAHVIGDWYRRR
                     YDRATVYIPYGCDGRTLPAGETLARFGLRPEEYLLYVSRLEWENNADVVIRAYRAAGG
                     LETLGVPLVVVGDAPYANAYKRRLETLAGITPGVMLTGYVFGDGYVELQSNALAYVQA
                     TQVGGTHPVLVEAMGRGAFVIANDVPEHREVLAEAGLYYARNDPRSLAQALQTARGDA
                     GLRKRLGAAAAERARALYSWEHVTDEYERLFLDLAGPAEARASA"
     gene            1317..2432
                     /locus_tag="VIF63_02460"
     CDS             1317..2432
                     /locus_tag="VIF63_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY8238273.1"
                     /translation="MPAHHDRMRVGLDAHVVGHQRTGNETYVLGLAHALVGHPEIDLT
                     AYVDRGVDWPIDGHTPAPRLRRLIARRPEPRIALELPLRAVRDRLDVLHVQYVAPLIS
                     SVPIATIVHDLSFFDVPDDLSAARRWRLRILVALAARGSKVVITPSEFSRERLLHHVN
                     VPPERVMVAPPAVLPPADPPDAAGILAGLHVPARFILAVGDVHPRKNHARLIEATAQA
                     RRQGLDISLVIAGQRGFQAGSLDVAVAANDAAAWVHLLGYVDRAALGALYRAAMVVAY
                     VSTYEGFGLPVVEAMAAGTPVVASGVTSVPEVAGDGAVLVDPTSVDDICRGLLAAATD
                     EALRERLIVAGRLRAEAYGPDAVVGATLAAYRHALGS"
     gene            2483..3388
                     /locus_tag="VIF63_02465"
     CDS             2483..3388
                     /locus_tag="VIF63_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238274.1"
                     /translation="MTETANRPPISVTISTVQGWPDVRDAILEAAASVRRAGGELIVT
                     DGSGLPAPGPDELGEGVRWLSFAGQSVFQLRERGYEAATGSVVAVTEDHVHVPEEWAA
                     LMVAAHAQHPEATAIGGSVVNAATGGLMDWGSFLIVQAPVAAPIRSGPARRLSGAVNV
                     AYKRAAIEHIDDFGGLGAMDGLHQRNLMKSGAVLINDDSIRVRHDQSLGFRGTTAIHY
                     NAGRTISGFKRAHLGLVDVIRIAGAPFVPLARYVRTVLLLAPRGYGPLLIRTTPAILW
                     LLYSQGVGQLLGYLLGPGDSPGKVQ"
     gene            3468..5015
                     /locus_tag="VIF63_02470"
     CDS             3468..5015
                     /locus_tag="VIF63_02470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8238275.1"
                     /translation="MNATPPPPPSRQGPGDPSIRRTWWLLAGSFLVLAVTNGLRLLID
                     QQPIDWLTVGLVFVAAIAAVAHRNAVGGLESRGRGEAESFARILRGLSRSVSAEAIVS
                     AIVDDLIAATGADHVVLVRRRRDGAALEATLVTRRAGVPTTTTVLPSSLLELPLDGDP
                     KPLVAVPIGPVEVEMPAFEAAEVAVGATADATDAPDAADATAATAAADAADGTSAGLG
                     GATPLPRVARRASDVGLGRTFVRRLAAEGLALLRDIGLPTPDIFGARGTAKPAEVLAR
                     GDEAIVAERIADRVKSAFALSHTLTVPLRTDAGVLGAIVLSRRDRESWPHSARRLMRV
                     AATETSAALDRADSFRAAETSASTDPLTGLPNRRYFEEFSSLLSERRRAGDALAVLMI
                     DIDKFKGLNDTYGHPAGDEVLRSVAGAITAAVRDQDVPARVGGEEFAVLLRNPGPDVA
                     LEVGERVRRAVRDLDLGDLGVPGVSVSVGVANATGPEEPIHALVDRADRALLRAKRAG
                     RDRVIAG"
     gene            5305..7290
                     /gene="polX"
                     /locus_tag="VIF63_02475"
     CDS             5305..7290
                     /gene="polX"
                     /locus_tag="VIF63_02475"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEY8238276.1"
                     /translation="MLEGSDARRDDAAFADEEGAAEEAIVGGAEAGAGAGRVGDGKDS
                     AGAAAEPGEVPLLTNGQLAQVFHDIGDLLEVKGELVFKTVAYHRAADTIGRQPIEVSK
                     AYREGKPPDIPGVGKSMAGQLAELATTGRSEYREKLLTEFPTSLLEMLRLPGVGPKTV
                     RLIYNELGVKTVDELRVAVEQKRVRTLRGLSERTEQLILEGIAKLEKRDRRMLLNQAK
                     ALIDELSGPLAQVLGVGRIVPAGSYRRRRESIGDLDLLAETDRPEELVEAFVKLPSIE
                     AVIGKGAHKAAVRLGGRGPQVDLMLMRPEQAGTHLIHFTGSKEHNVRLRERARDTGWS
                     LSEYGFQKIGEDGEALTGADAELRTFATETEAYAFLGLPFIEPELREDRGEIEAALAG
                     TLPDLIAESDLRGDLHSHSDWSDGSQPVEVMAEFARRRGHAYQVMTDHTVSLAIARGL
                     TPERVAQERALIRSLNKRFEAEERAGTAPAETSPEGFRLLHGCELEIRADGQLDYPDK
                     LLATFDLVVASLHVGRRQPRAELTQRVLNAIENPNVDVIAHPAGRMIDTRDDLDLDWD
                     TVFRAAARTGTVLEMNGSPHRLDLSVERARRALELGCYLSIDSDAHRTSEFEHLAWGI
                     SQARRAWVTQADVLNTRSRADLLAWLASPKPRVPSRP"
     gene            7307..8122
                     /locus_tag="VIF63_02480"
     CDS             7307..8122
                     /locus_tag="VIF63_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238277.1"
                     /translation="MKGESGARDLAIGVAVVIALSRFAPDAFAWPIAVVLLAGVILGA
                     LQVVAEADPAASAKGVAIESLILPGAAALAALGVIRLVPIGIVLAPAFALAGGIVGLA
                     LVTELRLARASGPPSSADRSAVLIQVLLVGFLGFAGVASLVPGGLPVPGAPAEVPGSN
                     PLAVVAQALGDAVIAFLLAYRVAALRSSNLRDVSWFGLTGAAVVAIAAVALRSIEIPR
                     ILGPALLVLVFFLWDAIHSGGAVRRRDPWRAWETAALAILAIVVIGWSAGIRA"
     gene            8307..9161
                     /locus_tag="VIF63_02485"
     CDS             8307..9161
                     /locus_tag="VIF63_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8238278.1"
                     /translation="MTFSETEANSSPAAATRMKRQLAEQAIASAAAGKWAEAADINRR
                     LLEMGAEAESENRLAKALWEQGELGSAREHYQAALALDPTNRIAERNIDRLRTLLVAA
                     GDKTVPAQEGSKAPVRIFVEETGKTGFAHLLELPEAKKLAQVNPGDSVELTPEGNRLI
                     AFSNGMRIGVVEPRVAARLLKLIAEGNKYLAGVTSLGAQDVRIIIREIYQDPRNYGKV
                     SFPTAAKSTDLRPYTKGTLLREDEELEEDLEDDVEDEEITDLDRVLPPEDTTDEAFIE
                     ETDENDEP"
     gene            9693..>12013
                     /locus_tag="VIF63_02490"
     CDS             9693..>12013
                     /locus_tag="VIF63_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238279.1"
                     /translation="MAGRLLLSRAESGFTLPAFERLAPPPGPELVGARLEANSAPGDI
                     VADLFGRGGWIARAAIDRQRRAFSLESSPLTRLLAEIVLRPPDLRHLDAAFSSLSASS
                     YAESSLRLAIADMFGTKCRTCGRNVPIDEAEWRDAAPDAEPTAEPTAAATSDIDGEPR
                     ATRRKSPRAKAGPQLAKLHYRCALCRDQQARSEHQAVEPLDADNERAARDVGAADVRR
                     HLRERFPVPDGGDHLIDAILDLHTDRQLVGLAAILARVEGDLRAAPVESALRLAFLHA
                     VLPASRLGLGGSRIPTLRLASGTVRSTTSGTWRERNPWLAFEDGFRQVRSFVQRLESG
                     TFIPLEARFGMDLRSLVESSSNTVIRVMTPGALDTLAREPGPKIRLVIGQPPARFSQE
                     RLGAAYHGTCWALGREAASLLPLEPLLGSAVRAPWSWQAAALRRSLEAVVPHLAGDAR
                     VILLLEHGGPEALIAAVLGGVGAGYRVLEARLTEPDDEVGGLVELIPPGARLPPGSLT
                     RSNRRLEHGPGGRGDPDVVPGPGLFAPPEHIEARPFSAKEAAATVLETAIDVLQARGE
                     PARHERLLGEILVELDRSGQLRRLMATDSTAAPDLPDRRADPDPLPEDLGPAFDATPG
                     TLGAAAKASAGAGGSALAGTRTRIFSQPPEPRDPVERVLALVGNELNRTDQRRVVEIE
                     PGRWWLGERRDREAAAIPLADRVEWAVYSLLSTAGPIAETAFFERVAALFSGHDLPDE
                     TLVRACLASYRSPASTPSRLVTSEDLRKRTDEHT"
BASE COUNT         1716 a         4400 c         4160 g         1737 t
ORIGIN      
        1 acgagcctgg aactcctcat gtccggaccc tacgggtcag atcagggcgc gtcatcggcc
       61 cagtaggtca aaaggggctg gtactagcgt gcggtgccgg gcgacaacgg gccgcaccgc
      121 atccagggaa cgttacgcgg aagcgcgggc ctcggccggc ccggccaggt ccaggaagag
      181 gcgctcgtac tcgtcggtca cgtgctccca cgagtagagc gcgcgcgccc gctcggcagc
      241 cgccgccccc agccgctttc gaaggccggc gtcaccacgc gcggtctgca gggcctgggc
      301 gagggacctc ggatcgttcc gggcgtagta cagcccggcc tcggccagca cctcgcgatg
      361 ctcggggacg tcgttcgcga tcacgaacgc gccgcggccc atcgcctcca cgaggacggg
      421 atgcgtcccg ccgacctgcg tcgcctgcac gtacgcgaga gcgttcgatt ggagctcgac
      481 gtacccgtcg ccgaagacgt agccggtcag catcacgccc ggcgtgatgc cggcaagcgt
      541 ctcgagccgg cgcttgtagg cgttcgcata tggcgcatcg ccgacgacga cgagcggcac
      601 gccgagggtc tcgaggcccc cggccgcgcg gtacgcccgg atcacgacat ccgcgttgtt
      661 ctcccactcc aggcggctga catacagcag gtattcctcg ggccgcagtc cgaaccgggc
      721 gagggtctcg ccggcgggca gggtccgtcc gtcgcagccg tatgggatgt acacggtcgc
      781 ccggtcgtag cgtcgtcgat accaatcgcc gatcacgtgc gcgtccgcga tgagcacgat
      841 cggcagcttc ggcgccagcc acgcgcacgc ctgtgcatac cagcgcccga gcgagttcca
      901 cttcccccgc tcccactcga ggccatccgt gttgagcacg accttcgccc cacccaggcg
      961 cggcacgagc gcgaacggcg cattggcgtt gttgcagatg aggacgacgt cgtaccgccg
     1021 ggggagggca tcgaggaccg agatggcacc gtggagcacg gtgtcgaggt acttgtggcg
     1081 aatcgtcggc aggcgccgga tgcggatgcc gcggtgggtc gtgatgccct tcgggacgac
     1141 gtgggaccgc ccgtacacgg tgacttcgtg gccgcgctgg accagtcgcg ctccgagctc
     1201 ctcggcgaac gtctcgaaac cgccatacga cgccgggacg ccgcgggttc cgaggatggc
     1261 gatgcgcatc gccggatcgt aggggagttg tgcggtcgct atgccatcat gcccgggtgc
     1321 cggcccacca cgatcgaatg cgggtcgggc tggacgccca cgtcgttggc caccagcgga
     1381 ccggcaacga gacctacgtc ctcggccttg cccacgcgct cgtgggccac ccggagatcg
     1441 acctcaccgc gtacgtggac cgcggcgtcg actggccgat cgacgggcac accccagccc
     1501 cacgcctgcg caggctcatc gcgcgccgcc ccgagccacg gatcgcgctg gagctgccgc
     1561 tgcgcgcggt acgcgatcga ttggacgtcc tgcacgtcca atacgtcgcc ccgctgatct
     1621 cgtcggtgcc gatcgcgacg atcgtccacg acctctcgtt cttcgacgta cccgacgacc
     1681 tgtccgcggc ccgacgctgg cgactgcgaa tcctggtggc cctcgcggcc cgcggatcga
     1741 aggtcgtcat cacgccgtcg gagttctcgc gggagcgact cctccatcac gtcaacgtgc
     1801 cccccgagcg cgtcatggtg gcgccacccg ctgtccttcc tccggccgat ccgcccgacg
     1861 ccgcgggcat cctcgcgggc ctccacgtgc ctgcgcgatt catccttgcc gtcggcgacg
     1921 tccacccgcg caagaaccac gcccgactga tcgaggcgac cgcgcaggca cgccggcaag
     1981 gcctcgacat ctcgctggtg atcgccggcc agcgaggctt tcaagccggg tcgctggacg
     2041 tggcggtggc ggcgaacgac gcggccgctt gggtgcatct cctcggctac gtcgatcgtg
     2101 cggcgctcgg tgcgctctac cgcgcggcga tggtcgtcgc ctacgtctcg acgtacgagg
     2161 gcttcgggct gccggtcgtc gaggcgatgg ccgccggcac cccggtcgtc gccagtggtg
     2221 tcacgtcggt gccggaggtc gccggcgacg gggcggtcct ggtcgacccg acgagcgttg
     2281 acgacatctg tcgcgggctg ctcgccgcgg caacggacga ggcgctgcgc gagcgattga
     2341 tcgtcgccgg ccggctacga gccgaggcgt acggcccgga tgccgtggtc ggcgcgaccc
     2401 tggcggcata ccgccacgcc ctgggctcct gaccacgtac gattcggagc gatccaccaa
     2461 ctccatgcga ggcgatccgc ggatgaccga aacggcaaac cgcccgccca tcagcgtcac
     2521 gatcagcacc gtccagggct ggcccgacgt gcgggatgcg atcctcgaag ccgccgcgag
     2581 cgtgcgccgc gctggtggcg agctcatcgt caccgatggc agcgggctgc ccgcgccggg
     2641 accagacgag ctgggcgagg gcgtgcgctg gctcagcttt gccgggcagt ccgtcttcca
     2701 gctccgtgag cgcgggtacg aggccgcgac cgggtcggtc gtcgccgtca ccgaggatca
     2761 cgtgcacgtc ccggaggaat gggcagcgct catggttgcg gcccatgcgc agcacccgga
     2821 ggcgaccgcg atcggtgggt cggtcgtgaa cgcggcgacc ggtggcctca tggactgggg
     2881 cagcttcctc atcgtccagg cgccggtggc ggcgccgatc cggagcggcc cggcgcggcg
     2941 cctgtcaggc gcggtcaacg tcgcgtacaa gcgcgcggcg atcgagcaca tcgacgactt
     3001 cggcgggctc ggcgccatgg acggcctgca ccagcgcaac ctgatgaagt cgggcgccgt
     3061 gctcatcaac gacgattcga tccgcgtgcg ccatgaccag tcgctgggat tccggggcac
     3121 gacggcgatc cactacaacg ccggccggac gatctcgggg ttcaagcggg cgcacctcgg
     3181 actcgtggac gtcatccgga tcgctggcgc gcccttcgtc ccgctggcgc ggtacgtccg
     3241 gaccgtgctc ctgctggccc cgcgaggcta cgggcccctc ctgattcgca ccacgccggc
     3301 gatcctgtgg ctgctctatt cccagggcgt cggccagctg ctgggctacc tcctcgggcc
     3361 aggcgacagc ccgggcaagg tccaataggc ggcggtgccg ccgggcaccc ctcgcagctc
     3421 cagcgctgga ctgttgccgg tcggacggct catccttcgg gcatatcgtg aatgccaccc
     3481 cacctcctcc cccaagccgg caaggacccg gggatccgtc gattcgtcga acgtggtggc
     3541 tgctggcggg ctccttcctt gtcctcgccg tcacgaatgg gcttcggctt ctcatcgacc
     3601 agcaaccgat cgactggctg acggtcgggc tcgtcttcgt cgcggcgatc gcggcggttg
     3661 cccatcggaa cgcggttggc ggcctggaat cacggggccg cggcgaggcc gagagcttcg
     3721 cccggatcct gcgcggcctg tcgcgctccg tctcggccga ggcaatcgtg tcggcgatcg
     3781 tcgacgacct gatcgcggcg accggcgcgg accacgtggt gctggttcga cgacgccgcg
     3841 acggtgcggc gctcgaggcg acgctcgtca cgcgccgcgc cggcgtgccg acgacgacaa
     3901 ccgtcctgcc gtcctccctc ctcgagctgc cgctcgacgg cgacccgaaa ccgctcgtgg
     3961 cggtgccgat cggacccgtt gaagttgaga tgccggcgtt cgaggcggcc gaggtcgccg
     4021 tcggcgccac cgcggacgca acggacgcgc cggacgccgc tgacgccacc gccgcaaccg
     4081 ccgccgccga cgctgccgac gggacgagcg cagggctcgg tggcgcgaca ccgctgccac
     4141 gcgttgcccg ccgcgcatcc gacgtcgggc ttggtcggac gttcgtgcgg cggttggcgg
     4201 cagagggcct ggcgctcctg cgtgacatcg ggctgccgac gccggacatc ttcggcgcac
     4261 gcgggaccgc caagccggcc gaggtgctcg cgcgcggcga cgaggcgatc gtggccgaac
     4321 ggatcgcgga tcgagtcaag tccgcattcg ccctctcgca cacgctgacg gtgccactgc
     4381 ggaccgatgc cggtgtcctc ggtgcgatcg tcctctcgcg ccgcgatcgc gaatcgtggc
     4441 cgcacagcgc gcggcggctc atgcgcgtgg ccgcgacgga aacgtcggcc gcgctcgacc
     4501 gcgcggattc cttccgagcc gccgagacgt cggcctcgac cgacccgctc accggcctgc
     4561 cgaaccgccg ctacttcgag gagttctcgt cactgctctc ggagcgccgc cgggcgggtg
     4621 acgcactggc cgtgctgatg atcgacatcg acaagttcaa gggcctcaac gacacctacg
     4681 gccacccggc gggcgacgag gtcctgcgct ccgttgccgg cgcgatcacc gccgcggtcc
     4741 gcgaccagga cgtgccggca cgcgtcggcg gcgaggagtt cgcggtcctc ctccgcaatc
     4801 ccggcccgga cgtggcgctc gaggtggggg agcgcgttcg acgtgcagtc cgcgacctcg
     4861 acctgggcga cctcggcgtg cccggcgtca gcgtgtccgt cggcgtcgcc aacgcgacgg
     4921 gccccgaaga accgatccac gccctcgtcg accgcgccga ccgggcgttg ctccgagcca
     4981 agcgcgccgg ccgcgaccgg gtgatcgccg gctagctcgt actcgaggaa ggtgctggcc
     5041 cgcggcggtc gcaattgcgg gcggttgcgc catacgtttc cgtccggcac gttcggtcgc
     5101 tgccgcaccc tcaaacggcc catttcgggc atcgccatcc cgcccgaacg cttggttgag
     5161 cacgattcga gcgtgcgtgg tcgcctggag tcagtgcgag ctcggctttc tgcgctctaa
     5221 cgtgcccaag gaatgcatat ggcgcggatg cgccgaagcg gccctgtgaa cgggtccgcc
     5281 aagccggctc gataccatcg acgcgtgctc gaaggatcgg acgcgcgcag agacgacgcc
     5341 gcgttcgcgg atgaggaagg cgccgccgag gaggccatcg tcggtggcgc ggaggcgggc
     5401 gccggcgccg gccgggtcgg cgacgggaag gacagcgcgg gcgcagccgc cgagccgggc
     5461 gaggtgccgc tcctgacaaa cggccagttg gcgcaggtgt tccacgacat cggtgacctg
     5521 ctcgaggtca agggcgagct cgtgttcaag accgtcgcgt accaccgcgc ggcggacacg
     5581 atcgggcgac agccgatcga ggtgtcgaag gcgtatcgcg agggcaagcc gcccgacatc
     5641 ccgggcgtcg gcaagtcgat ggccggccag ttggccgagc tggcgacgac cggccggagc
     5701 gagtaccgcg agaagctcct caccgagttc ccgacgagcc tgctcgagat gctgcgcctg
     5761 cccggcgtcg ggccgaagac cgttcgactg atctacaacg agctcggcgt caagaccgtc
     5821 gacgagctgc gcgtcgcggt cgagcagaag cgggtccgga ccctgcgcgg cctgtcggag
     5881 cggaccgagc agctgatcct cgagggcatc gccaagctcg agaagcgcga tcggcggatg
     5941 ctcctgaacc aggcgaaggc gctgatcgac gagctgtccg ggccgttggc gcaggtcctt
     6001 ggtgtcgggc ggatcgtgcc ggccgggtcg taccgacgac gacgggagtc gatcggcgac
     6061 ctggacctgc tcgcggagac ggatcgcccc gaggagctgg tcgaggcgtt cgtgaagctg
     6121 ccgtcgatcg aggccgtcat cggcaagggc gcgcacaagg cggcggttcg cctcggcggg
     6181 cgcggcccgc aggtcgacct gatgctgatg cggccagagc aggcgggcac gcacctcatc
     6241 cacttcacgg gctcgaagga gcacaacgtc cggctccgcg agcgtgcgcg agacactggc
     6301 tggagcctgt ccgagtacgg gttccagaag atcggcgagg acggcgaggc gctgacgggg
     6361 gccgacgcgg agctcaggac gttcgcgacc gaaaccgagg cctatgcgtt cctcgggctg
     6421 ccgttcatcg agccggagct gcgcgaggat cgcggcgaga tcgaggcagc gttggccggg
     6481 accttgcccg atctcatcgc cgaatcggac ctccgcggcg acctccactc ccattcggac
     6541 tggtccgacg gcagccagcc cgtcgaggtc atggccgagt ttgcgcgccg tcgcggccac
     6601 gcctaccagg tgatgaccga ccacaccgtg tcgctggcga tcgcccgcgg cctgaccccc
     6661 gagcgggtcg cacaggagcg agccttgatc cgctccctca acaaacgctt cgaggcagag
     6721 gaaagggccg gcacggcgcc cgccgagacg tcgcccgagg ggtttcgcct cctgcacggc
     6781 tgcgagctcg agatccgcgc cgacggccag ctcgactacc cggacaagct gctcgcgacg
     6841 ttcgacctcg tcgtcgcctc gctgcatgtc ggtcggcgac agccgcgagc ggagctcacg
     6901 cagcgggtcc ttaacgccat cgagaacccg aacgtcgatg tcatcgccca tccggctggc
     6961 cggatgatcg acacccgcga cgacctcgac ctcgattggg acacggtctt ccgagccgcc
     7021 gcgcgaaccg gcacggtgct cgagatgaac ggctcgccgc atcgactcga cctgtccgtc
     7081 gagcgggcgc gccgggcatt ggagctcggc tgctacctct cgatcgactc ggacgcgcat
     7141 cgaacgtcgg agttcgagca cctggcgtgg ggcatctcgc aggcccgtcg tgcatgggtg
     7201 acgcaggcgg acgtgctgaa cacgcgctcc cgtgccgacc tcctcgcctg gctcgcatcg
     7261 ccgaagcccc gcgtaccatc ccggccgtga acccgagcgg cacgcgatga agggcgaatc
     7321 cggcgcgcgc gacctcgcga tcggcgtcgc cgtcgtcatc gcgctgtcgc ggttcgcgcc
     7381 cgatgccttc gcctggccga tcgcggtcgt gctgctcgca ggcgtgattc tcggcgccct
     7441 ccaggtcgtg gccgaggccg atcccgccgc atcggccaag ggcgttgcca tcgaatccct
     7501 gatcctgccg ggtgccgccg cgctggcggc cctcggcgtg atccgcctcg tcccgatcgg
     7561 gatcgtgctg gcgcccgcat tcgcgctcgc cggcggcatc gtcgggctgg cgctcgtcac
     7621 cgagctgcga ctcgcccgcg cgagcgggcc accgtcgagc gccgatcgat ccgcggtcct
     7681 catccaggtc ctgctcgtcg ggttcctcgg cttcgccgga gtggcgtcgc tggtgccggg
     7741 cggcctgccg gtccccggtg cacccgcgga ggtgcccggt tcgaacccgc tcgccgtcgt
     7801 ggcgcaagcc ctcggcgacg ccgtcatcgc cttcctgctc gcgtatcgcg tcgccgcgct
     7861 ccggtcgtcg aacctgcggg acgtctcctg gttcgggctg accggcgctg cggtcgtggc
     7921 gatcgccgcg gtcgccctcc gttccatcga gattccgcgg atcctcggcc ccgcgctcct
     7981 cgtgctcgtc ttcttcctgt gggacgccat ccacagtggg ggcgcggttc gacggcgtga
     8041 tccgtggcgc gcctgggaga cggccgccct cgcgattctc gccatcgtcg tgatcggctg
     8101 gagcgccggc atccgcgcct gaacggtcag aattcgggcc gattcgggct cctcggcgac
     8161 cgattcggcg cccttgcgcg acctccgcgg cgcgaggccc catacttgcg caccgcgtcg
     8221 cgctcgctat cgttgcgcgc cgcacacctc gagggccgcg tccgcgcgac cccgacgccc
     8281 agatcgacat cgatgaggac ccatccgtga ccttctccga gaccgaagcg aactcaagcc
     8341 ccgcagccgc gacgcgcatg aagcgccagc tcgccgagca ggcgatcgcc agcgccgccg
     8401 ccggcaagtg ggccgaggcc gccgacatca accgccgcct gctcgagatg ggcgccgagg
     8461 ccgagtccga gaaccggctt gcgaaggcgc tctgggagca gggcgagctc gggtccgccc
     8521 gcgagcatta ccaggccgcg ctcgccctcg acccgacgaa ccgcatcgcc gagcgcaaca
     8581 tcgatcggct gcgcaccctc ctcgtcgcgg ccggcgacaa gaccgtgccg gcgcaggagg
     8641 gctcgaaggc cccggtccgc atcttcgtcg aggagaccgg gaagaccgga tttgcccacc
     8701 ttctcgagct ccccgaggcc aagaagctgg cccaggtcaa cccgggcgac tcggtcgagc
     8761 tcacgcccga gggcaaccgg ctgatcgcat tcagcaacgg catgcgcatc ggcgtcgtcg
     8821 agccacgcgt ggcggcccgc ctgctcaagc tgatcgccga aggcaacaag tacctcgccg
     8881 gcgtgacctc gctcggcgcg caggacgtcc ggatcatcat ccgcgagatc taccaggacc
     8941 cgcgtaacta cggcaaggtc tcgttcccga ccgcagcgaa gtccaccgac ctccggccct
     9001 acaccaaggg caccctcctg cgcgaggacg aggagctcga ggaagacctc gaggacgacg
     9061 tcgaggacga ggagatcacc gacctcgacc gtgtcctccc gcccgaggac acgactgacg
     9121 aggccttcat cgaggagacc gacgagaacg acgagccgta accgcctcgc ggatgcggcc
     9181 gtttcgcagc gttgtcgctt gcgcgcgctc gctcacgcac cctgaggtgc gctcgctctc
     9241 gcgctcggct ccgccttgcg ctccgaccgc ctccgcgagg cggtagctcg tcctcgcgtc
     9301 ctcaaacctc cagcggccgc tccgttcgcc gctgccactc ctcgctccca gccattcgcg
     9361 ggccgatgtt cactacatga acagaatcgg cacttgcgac gctatttcgc ctcgcgcggg
     9421 tgcgcgcagg cccgagtcgg cgccaattcg agcacgagtc gaggcgggct gagcgcgaat
     9481 tcgtcgctcg ccggcggggt ttggcccgcg cgcggcgcca aaagcctccc caagcgccag
     9541 aaatgatcat gccgtgaaca tcgccggtca cttggtcgga tcggagcggc tcgacgcgaa
     9601 gccgggcaag cgcgcacctc gctggcctcc gacgtctcgt gccacggcgc ccgtcgatca
     9661 gcgctgaatc gacctgacac aatccacgct tgatggcggg gcgactgctc ctctcgcgcg
     9721 cggagtccgg gttcacgctg cccgcgttcg agcggctcgc gccgccgcct gggccggagc
     9781 tggtcggtgc gcggctcgag gcaaattcgg cgccgggcga catcgtcgcc gacctgttcg
     9841 ggcgcggcgg ctggatcgcc cgggcggcga tcgatcgaca gcggcgtgcg ttcagcctcg
     9901 agtcgagccc gctgacacga ctgctcgcgg agatcgtgct ccggccgcct gacttgcgtc
     9961 acctcgacgc cgcgttctcg tcgctgtccg catcgtcgta cgccgagtcg agcctgcggc
    10021 tcgcgatcgc agacatgttc gggacgaagt gccggacgtg cgggcgcaac gtgccgatcg
    10081 acgaagcgga gtggcgcgac gcggcgcccg atgcagagcc cactgcggag cccactgcgg
    10141 cagcaacctc ggacatcgac ggcgagcctc gcgcgaccag gcggaagtcg ccccgcgcga
    10201 aggcgggccc gcagctcgcg aagctccact accgctgcgc gttgtgccgc gaccaacagg
    10261 cgcgatcgga acaccaggct gtcgagcccc tcgacgccga caacgagcgc gcggcccgcg
    10321 atgtgggagc ggcggacgtc aggcggcacc tccgcgagcg cttccccgtg cccgacggcg
    10381 gcgaccacct gatcgatgcc atcctcgacc tgcacaccga tcgtcagctc gtcgggctcg
    10441 cggcgatcct cgcccgcgtc gaaggcgatc tccgggccgc gccggtcgaa tcggcgctca
    10501 ggctcgcgtt cctccacgcc gtgctccccg ccagccgact cggcctcggc ggctcgcgaa
    10561 ttccgacgct ccgcctcgcc agcggcaccg ttcggagcac gacgtccggt acgtggcgcg
    10621 aacgcaatcc atggctcgcg ttcgaggatg gcttccgcca ggtccgaagc ttcgtgcagc
    10681 gtctcgagag cgggaccttc atcccgctgg aggcgagatt cgggatggac ctgcggagcc
    10741 tcgtcgagag ctcgtcgaac accgtgatcc gcgtgatgac gccgggcgcg ctcgacaccc
    10801 tggcccgcga gcccggcccg aagatccgac tggtcatcgg ccagccgccc gcccggttca
    10861 gccaggagcg gctcggggcg gcgtaccacg gcacgtgctg ggcgctcggg cgcgaggcgg
    10921 cctcgctcct gccgctggag ccgttgctgg gatcggcggt tcgggcgccg tggtcgtggc
    10981 aggcggccgc gctgcggcgg tcactcgagg cggtcgtgcc gcatctcgcg ggcgacgcgc
    11041 gcgtgatcct cctgctcgag cacggcggcc ccgaggcatt gattgccgcg gtcctcggcg
    11101 gcgtcggcgc gggctaccgc gtcctggagg cgcgcctgac cgagcccgac gacgaggtcg
    11161 ggggcctggt cgagctcatc ccacccggcg cgagactccc gccggggtcg ctcacgcgca
    11221 gcaaccggcg gctcgagcac ggacccggtg gccgcggcga ccccgatgtc gtccctggcc
    11281 ccggcctgtt cgcgccgccg gagcacatcg aagcgcgccc gttctccgcg aaggaggccg
    11341 ccgcaactgt cctcgaaacc gcgatcgacg tcctccaggc gcggggcgag cccgcacgcc
    11401 acgagcggct gctcggcgag atcctcgtcg agctcgatcg atccggccag ctccgccgtc
    11461 tcatggcgac cgattcgacc gccgcccccg acctgcccga tcgccgcgcg gatcccgacc
    11521 ccctccccga ggacctcggc cccgcgttcg atgccacgcc gggcaccctc ggcgcggccg
    11581 ccaaggcgag cgccggtgcg ggcgggagcg cgctcgccgg cacgaggacg aggatcttca
    11641 gccagcctcc ggagccccgc gatcccgtcg aacgcgtcct cgcccttgtc ggcaacgagc
    11701 tcaaccgaac cgaccagcgt cgggtcgtcg agatcgagcc gggtcgctgg tggctcggcg
    11761 aacggcgcga ccgcgaggcg gcggccatcc cgctggccga ccgcgtcgaa tgggccgtct
    11821 acagcctcct gtcgaccgcc ggcccgatcg ccgagaccgc gttcttcgag cgcgtggccg
    11881 cgctgttctc gggccacgac ctgccggacg agaccctcgt gcgggcgtgc ctcgccagct
    11941 accgcagccc ggcgtcaacg ccgagccgcc tcgtgacctc cgaggatctc cgcaagcgaa
    12001 ccgacgagca cac
//
LOCUS       DATBSW010000091         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_676858, whole genome shotgun sequence.
ACCESSION   DATBSW010000091 DATBSW010000000
VERSION     DATBSW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676858"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /gene="purS"
                     /locus_tag="VIF63_02495"
     CDS             complement(<1..245)
                     /gene="purS"
                     /locus_tag="VIF63_02495"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY8238280.1"
                     /translation="MSTFRFAVNVLPKPGILDPQGRAVEGSLSHLEVTGVSAVRVGRR
                     VELTVDDASGDAARATVERLASELLSNPLIEAYAIEAL"
     gene            complement(242..1123)
                     /locus_tag="VIF63_02500"
     CDS             complement(242..1123)
                     /locus_tag="VIF63_02500"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8238281.1"
                     /translation="MLTADRFLRSGKVRDLYEQPDGRLLLVASDRISAFDVILPTEIP
                     DKGRVLTGLSRFWFAETAALVPNHLLSTATDDPTLRGRVMVCRPTTVLPIEAVVRGYL
                     AGSGWKAYRASGAICGVTLPPGLRESDRLPAPIFTPATKAELGEHDENIDFERMAAIV
                     GDELAGRVRDLSIALYQHAAAVTERAGILLADTKFEFGVDAATGQLLLIDEALTPDSS
                     RFWDTATYAPGRPQASFDKQFVRDWLETQPWDKTAPGLALPPDVVEGTRARYVDAFER
                     ITGASFERYLQEDVIAP"
     gene            complement(1110..2441)
                     /gene="purB"
                     /locus_tag="VIF63_02505"
     CDS             complement(1110..2441)
                     /gene="purB"
                     /locus_tag="VIF63_02505"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8238282.1"
                     /translation="MIRRYTLAEMGAIWSDTARFEAMLRVELAVARAQAARGQVPAEA
                     LAALEDRARVDVERIAEIERTTEHDVIAFVSQVAETVGPEGRYLHLGLTSSDVVDTGL
                     ALQLQAAGRRLLEGTDRLLAVLVARARAEAETVMMGRTHSVHAEPTTFGLKLAGWAFE
                     VDRGRTRLAAAVDEIGTGKISGPVGTYSHLRPDVEAEVLAALGLHADPVSTQIVQRDR
                     HAALLTAIAVLGGSLERFATEIRNLQHTEIGEVQEPFAAGQKGSSAMPHKRNPIVSER
                     IAGLARLLRGYAHTALEDQPLWHERDISHSSAERVILPDATTVLDYMLVKMAALVEGL
                     VVRSERMRENIARGLGLHASSRVLTALVESDGLSREEAYAIVQRAALRAADERSPLRD
                     LLALDPTVAQKLSLAQLDACFDDRGFLGHVPAIIARLDRLVPTTEAAHAHG"
     gene            complement(2438..3769)
                     /gene="purD"
                     /locus_tag="VIF63_02510"
     CDS             complement(2438..3769)
                     /gene="purD"
                     /locus_tag="VIF63_02510"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8238283.1"
                     /translation="MPTRILIVGAGGREHALAWKLSAEPGVNEVIVAPGSGAIAEEPR
                     VRCIAVDPLDGAAVVALARREAVELVVVGPEAPLAAGVADALSGAGIAVFGPSQAAAR
                     IESSKAFCHEIATAAGVRMARAEAFTDVRAALAFAATLADAGRGVVIKADGLAAGKGV
                     TVCDDLETAAQAIVATLEDAGGVVVVEERLYGTEASLIALCDGSVALALPLARDHKRL
                     GDGDTGPNTGGMGAYSPLPDLSDEAIPGLIDAFHRPILAELARRGTPFRGALYAGLML
                     TADGPVLLECNARFGDPETQALLPRLAVALGPLLLAAARGQLGGAAAALKIDDSRLPA
                     IPGATVAVVLAAAGYPGTPRKGDLIEGLARARGLGTLVFHAGSFVVADGTARTVGGRV
                     LAVVGRGPDLATARDAAEDAADGITFDGLQRRHDIGLTAVTAQPELSGAAR"
     gene            complement(3787..5094)
                     /locus_tag="VIF63_02515"
     CDS             complement(3787..5094)
                     /locus_tag="VIF63_02515"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY8238284.1"
                     /translation="MPATVIVGLQWGDEGKGKTTDFLAEQVAMVVRYQGGDNAGHTVV
                     SGDEVLKLRLTPSGVLYPHITSVIGNGVVVNPVTLITELDMLASRGTDVSRVRVSRSA
                     HVIMPYHIALDRANEARLDGAKVGTTGRGIGPTYADRAWRLGLRMEDLLDRAVLRERI
                     GRVLLDKNLLLRSMGADPFEVDALVEEAAGWGERLRSHLDDTTWLVQAALGRGEHVLL
                     EGAQGTLLDLDHGSYPFVTSSNPVAGGACTGGGIGPLQVDEVIGVMKAYSTRVGSGPY
                     PTELHDDVGAGIAERGHEFGTVTGRPRRVGWFDAVPLRYAVAVNSVSSIMLNKLDILS
                     GVDPIKLCVAYEIDGRRVEVWPSSGAALARATPIYEDFPGWAEPIFDVRSLADLPENA
                     RRYVSAIEAHAGVPIVLVSVGPERTQTIERAWRPMRNRPGVPA"
BASE COUNT          712 a         1862 c         1896 g          715 t
ORIGIN      
        1 agcgcttcga tggcgtacgc ctcgatcagc gggttcgaga gcagctcgga tgcgagccgc
       61 tcgacggtcg cgcgcgccgc gtcccccgac gcgtcgtcga cggtcagttc gacccgccgc
      121 ccgacgcgca cggcgctgac gccggtgacc tcgaggtggc tgaggctgcc ttccacggcc
      181 cggccctggg ggtcgaggat gccgggcttg ggcaggacgt tgacggcgaa ccggaacgtg
      241 ctcatggggc gatgacgtcc tcctggaggt agcgctcgaa gctggcgccg gtgatgcgtt
      301 cgaaggcgtc gacgtagcgg gctctcgtgc cctcgacgac gtcgggcggc agcgccagac
      361 ccggcgcggt cttgtcccag ggctgggtct cgagccagtc ccgcacgaac tgcttgtcga
      421 agctcgcctg ggggcgaccc ggggcgtagg tcgcggtatc ccagaagcgc gacgagtcgg
      481 gcgtgagcgc ttcgtcgatc aggagcagct gaccggtcgc ggcgtctacg ccgaactcga
      541 acttggtgtc ggccaggagg atcccggcgc gctccgtgac ggcggccgcg tgctggtaca
      601 gcgcgatcga caggtcgcgg acgcgtcctg ccagctcgtc cccgacgatc gcggccatgc
      661 gctcgaagtc gatgttctcg tcgtgttcgc cgagctccgc tttcgtggcc ggcgtgaaga
      721 tcggcgccgg gagccggtcg ctctcgcgca ggccgggcgg cagcgtgacg ccgcagatcg
      781 cgccgctcgc ccggtacgcc ttccagcccg aaccggcgag atagccgcgg accacggcct
      841 cgatgggcag cacggtcgtc gggcggcaga ccatcacccg gccgcgcagg gtcgggtcgt
      901 ccgtcgccgt cgacagcagg tggttcggca cgagcgccgc ggtctcggcg aaccagaacc
      961 gcgacaggcc ggtcaggacg cggcccttgt cgggaatctc cgtgggcagg atcacgtcga
     1021 acgcgctgat ccgatcgctc gcgacgagca gcagccggcc atccggctgc tcgtagaggt
     1081 cgcggacctt gcccgagcgg aggaagcggt cagccgtgag catgggcggc ctccgtcgtc
     1141 gggaccaggc gatcgaggcg ggcgatgatc gccgggacgt ggcccaagaa gccgcggtcg
     1201 tcgaagcacg cgtcgagctg ggccagcgac agcttctgcg cgacggtcgg atcgagcgcc
     1261 agcaggtcgc gcagcggcga gcgctcatcc gcggcccgga gcgccgcgcg ctggacgatg
     1321 gcgtaggcct cctcgcgcga gaggccgtcg ctctcgacga gtgccgtgag gacccggctg
     1381 ctggcgtgca gcccaaggcc gcgcgcgatg ttctcgcgca tgcgctccga acggacgacg
     1441 agcccctcga ccagggcggc catcttgacc agcatgtagt cgaggacggt cgtcgcgtcc
     1501 gggaggatga cccgctcggc gctcgaatgg ctgatgtcgc gctcgtgcca gagcggctga
     1561 tcttcaaggg cggtgtgcgc atagccgcgc agcaggcggg cgagccccgc gatccgctcg
     1621 gacacgatgg ggttgcgctt gtggggcatc gcactggagc ccttctggcc cgcggcgaac
     1681 ggttcctgga cctcgccgat ctccgtgtgc tggaggttgc ggatctcggt ggcgaaccgt
     1741 tccagcgacc cacccaggac cgcgatggcg gtcagcagcg cggcgtgccg gtcgcgctgg
     1801 acgatctggg tgctgaccgg gtcggcgtgc aggccgaggg cggccaggac ttcggcctcg
     1861 acatcggggc gcaggtggct gtacgtcccg accggacccg agatcttgcc ggtgccgatc
     1921 tcgtcgacgg ccgcggcgag ccgcgttcgc ccacgatcga cctcgaacgc ccagccggcg
     1981 agcttgagtc cgaacgtcgt cggttcggcg tggacggagt gggttcgccc catcatcacc
     2041 gtctcggcct ccgcccgtgc ccgcgcgacg aggaccgcca ggagccgatc ggtgccctcc
     2101 agcagccgac ggccggcggc ctggagctgg agcgccagcc cggtgtcgac gacgtcgctg
     2161 ctggtcaggc cgaggtgcag gtagcgcccc tccgggccga ccgtctcggc gacctggctg
     2221 acgaaggcga tgacgtcgtg ctcggtcgtc cgctcgatct cggcgatgcg ctcgacatcg
     2281 acgcgcgcgc gatcctcgag ggcggcgagc gcctcggccg gcacctggcc gcgagcggcc
     2341 tgcgcgcggg ccaccgcgag ctcgacccgg agcatggcct cgaaccgggc ggtatccgac
     2401 cagatggccc ccatctcggc gagcgtgtag cgccggatca ccgggccgcc ccgctcagct
     2461 cgggctgcgc ggtcacggcg gtgagcccga tgtcgtggcg gcgctggagg ccgtcgaagg
     2521 tgatgccgtc ggccgcatcc tccgcggcgt cgcgggccgt cgccaggtcc ggcccgcggc
     2581 cgacgacggc gagcacgcgt ccacccaccg ttcgggccgt gccatcggcg acaacgaagc
     2641 tcccggcatg gaacaccagc gtgccaaggc cgcgtgcgcg ggccaggccc tcgatgaggt
     2701 cgcccttgcg tggcgtgccc ggatatccgg cagcggcgag gaccaccgcg accgtggcgc
     2761 ccgggatggc cgggagccgt gaatcgtcga tcttgagcgc ggcggcggct ccaccaagct
     2821 gcccacgggc cgcggcgagc agcagcggcc ccagggcgac cgccaggcgc ggcaggaggg
     2881 cctgcgtctc gggatcgccg aaccgcgcgt tgcactcgag caggaccggg ccgtcggcgg
     2941 tgagcatcag accggcgtac agggcgccac ggaacggggt cccgcgcctg gccagctcgg
     3001 cgaggatcgg acggtggaag gcatcgatca gccccgggat tgcctcgtcg gagaggtccg
     3061 ggagcgggct gtaggcgccc atgccgcccg tattcggccc ggtgtcgccg tcgcccaggc
     3121 gcttgtggtc acgtgccagg gggagggcca gcgcgacgct gccgtcgcac agggcgatga
     3181 ggctcgcctc cgtgccgtac agccgctcct cgacgaccac gacaccgccc gcatcctcga
     3241 gcgtcgcgac gatcgcctgc gccgccgtct cgaggtcgtc gcacaccgtc acgcctttgc
     3301 cggcggccag tccgtccgcc ttgatcacga cgccccgccc ggcgtccgcc agggtcgcgg
     3361 cgaacgccag cgccgcgcgg acgtcggtga acgcctcggc acgggccatc cggacgcccg
     3421 ccgcggtcgc gatctcgtgg cagaacgcct tgctgctctc gatgcgcgcc gccgcctggc
     3481 tcggcccgaa gaccgcgatg ccggccccgc tgagcgcatc ggccacgccg gccgcgagcg
     3541 gggcctcggg ccccacgacc acgagttcga ccgcctcccg acgggcgagt gccacgaccg
     3601 cggctccgtc gagcggatcc accgcgatgc agcgcacgcg cggttcctcg gcgatggctc
     3661 cgctcccggg cgccacgatg acctcgttga cccccggttc ggcgctcagc ttccaggcca
     3721 gcgcatgctc ccggccaccc gccccgacga tgaggatccg tgtgggcatc aggaggctgg
     3781 ccgcgctcac gccgggaccc ccggccggtt gcgcatcggg cgccaggccc gttcgatggt
     3841 ctgggtccgc tccggcccga ccgacacgag gacgatcggg accccggcat gggcctcgat
     3901 ggcgctgacg tagcgtcgcg cgttctctgg caggtcggcc agcgagcgga cgtcgaagat
     3961 cggctccgcc caccccggga agtcctcgta gatcggcgtc gcgcgagcca gcgcggcccc
     4021 ggaagacggc cagacctcga cccggcggcc gtcgatctcg taggccacgc acagcttgat
     4081 ggggtccacg ccggacagga tgtcgagctt gttgagcatg atcgagctga cgctgttgac
     4141 ggccacggcg tagcgcagcg ggacggcgtc gaaccagccc acccgtcgcg gccggcccgt
     4201 gacggtcccg aattcgtggc cgcgctcggc gatgccggcg ccgacgtcgt cgtggagctc
     4261 ggtcggatac gggcccgacc cgacgcgggt cgagtacgcc ttcatgaccc cgatcacttc
     4321 gtcgacctgg agcggcccga tgccgccccc ggtgcacgcc ccaccggcga ccgggttcga
     4381 cgacgtcacg aacggatagc tgccgtggtc gagatcgagc agcgtcccct gcgcgccttc
     4441 gaggagcacg tgctccccgc ggcccagcgc cgcctgcacc agccacgtcg tgtcgtcgag
     4501 gtgcgagcgg agccgttcgc cccagccggc ggcctcctcg accagggcgt cgacctcgaa
     4561 gggatcggcg cccatcgagc gcaggagcag gttcttgtcg agcaggacgc gaccgatccg
     4621 ctcgcgcagc accgcacgat cgagcaggtc ctccatgcgc agcccgagtc gccacgcacg
     4681 gtcggcgtag gtcggcccga tccctcgtcc ggtcgtgccc accttcgcgc cgtccaggcg
     4741 cgcctcgttg gcccggtcga gggcgatgtg atacggcatg atcacgtgcg cgctgcggct
     4801 cacacggacc cggctcacat cggtgccccg cgaggcgagc atgtcgagct cggtgatgag
     4861 cgtgaccggg ttgacgacga caccgtttcc gatgaccgag gtgatgtgcg gatacagcac
     4921 gcccgacggt gtcaggcgga gcttgaggac ctcgtcgccg ctgaccaccg tgtggccggc
     4981 gttgtcgcca ccctgatagc ggacgaccat ggcgacctgc tcggcgagga agtcggtcgt
     5041 cttgcccttg ccctcgtcgc cccactggag cccgacgatc acggtggccg gcatcagccg
     5101 cgcaccttcg atcggtcggc gaaggcgagc ccggccatcg cggtcagggc ggaggcgaac
     5161 cgagaatcgg cgcgctgggc ggccg
//
LOCUS       DATBSW010000092         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_959344, whole genome shotgun sequence.
ACCESSION   DATBSW010000092 DATBSW010000000
VERSION     DATBSW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959344"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VIF63_02520"
     CDS             complement(<1..1183)
                     /locus_tag="VIF63_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8238285.1"
                     /translation="MSVYLDGRPVPAPSRLRFLAFGLAVVVAAGALSVRLFAIQVGGT
                     TPYTALAGTTRTVVEALPSSRGLIFDREGTPLVSNIASYSVKIRPSDLPESRRADVVQ
                     TLATLIGADATDINIAIDSNPGSRYDLVRVAQDIKPDVAAFIAESGTQLPGVEVVVET
                     RRNYEMGPLFAHVLGYTGPISGDELGDLRDSGYLPDDLLGRAGVESTYEDLLRGQYGL
                     QTVERDAAGREVQVLRTDRAPVAGDSLRLTLDIQEQKWAEQAMKWGMKAAGLKRGVVI
                     VMNPQNGEILALVSLPSYDDNQFSAGISNTQYQKLLNDPDKPLVNHAISDQYPPGSTY
                     KLIAGTGVLADGIITASTKIRTAGYLRLGGFIFRDWNDRGFGMCDIYCGFGHSSDTFF
                     YQ"
     gene            complement(1180..1680)
                     /gene="mreD"
                     /locus_tag="VIF63_02525"
     CDS             complement(1180..1680)
                     /gene="mreD"
                     /locus_tag="VIF63_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY8238286.1"
                     /translation="MSLLLAAIGATVIALVEATLAPYINSGNAGPHFVLIGGVIWTIA
                     AGIDRGITWAFVGGIVLDSLIGRPLGTSAFAMLVAVGGAALINQPFPRLRIVAPIVAV
                     PLLSLVYSFVLLTLISAYEPELNVPDPSSLFVPGAIYDAIIGIFLGPLVITLHDRRTV
                     TERMDW"
     gene            2067..>2520
                     /locus_tag="VIF63_02530"
     CDS             2067..>2520
                     /locus_tag="VIF63_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238287.1"
                     /translation="MGETIACANQKGGVGKTTTVVNLASYLALAGDRVLVVDLDPQGN
                     ATSAFGIDRTRDRSVYDAVIDQVQLEELAIDTAVDGVTLIPSSIALAGAEVELTPLEQ
                     RERRLATLLDPMRAKFDYLLIDCPPSLGLLTINALTAADSTLIPIQCEY"
BASE COUNT          449 a          793 c          870 g          408 t
ORIGIN      
        1 cctggtagaa gaacgtgtcg ctcgagtgcc cgaagccgca gtagatgtcg cacatgccga
       61 agccgcgatc gttccagtcg cggaagatga agccgccgag gcgcaggtag cccgcggtcc
      121 ggatcttggt tgaggcagtg atgatgccgt cggcgagcac gccggtgccg gcgatgagct
      181 tgtaggtgga gccgggcggg tactgatcgc tgatcgcgtg gttgaccagg ggcttgtccg
      241 gatcgttgag cagcttctgg tactgggtgt tgctgatgcc cgcgctgaac tggttgtcgt
      301 cgtagctcgg caggctgacc aacgcgagga tctcgccgtt ctgcgggttc atgacgatga
      361 ccacgccgcg cttgaggccg gccgccttca tgccccactt catcgcctgc tcggcccact
      421 tctgctcctg gatatcgagc gtgagccgaa gcgaatcgcc tgccaccggc gcccggtccg
      481 tccgcaggac ctggacctcg cgacctgctg cgtcccgctc gaccgtctgc aggccgtatt
      541 ggccgcggag gaggtcctcg tacgtcgact caacaccggc acgaccgagg aggtcgtcgg
      601 gcaggtagcc ggaatcgcgc aggtcgccga gctcgtcgcc gctgatcggg cccgtgtagc
      661 ccaggacgtg ggcaaacagc gggcccatct cgtagttgcg ccgcgtctcg acgacgacct
      721 cgacgcccgg cagctgtgtc cccgactcgg cgatgaacgc cgccacgtcg ggcttgatgt
      781 cctgtgcgac gcggacgagg tcgtagcgcg accctggatt cgagtcgatg gcgatgttga
      841 tgtccgtcgc gtccgccccg atgagggtgg ccagcgtctg caccacgtcg gcgcgccggg
      901 actcgggcaa gtccgagggc cggatcttga cgctgtacga cgcgatgttc gacacgagcg
      961 gcgtgccctc ccgatcgaag atcaggccgc gcgacgatgg gagtgcttcc acgacggttc
     1021 gcgtcgttcc ggcaagtgcc gtgtacggcg tcgtgccgcc gacctggatc gcaaacaggc
     1081 gcacggacag ggcgcccgca gccacgacga cggcgagccc gaaggcaagg aatcgcagtc
     1141 gcgaaggagc ggggaccggc cggccgtcga ggtacacgct caccagtcca tccgctcggt
     1201 cacggtccgt cggtcgtgga gcgtgatcac cagcggcccg aggaagatgc cgatgatcgc
     1261 gtcgtagatc gcgccgggca cgaacaggct cgatggatca gggacgttga gctctggctc
     1321 gtaggcggaa atgagggtca gcagcacgaa ggagtacacc aagctgagca ggggaacggc
     1381 gacgatgggg gcaacgatgc gcagccgcgg aaacggttga ttgatgaggg ctgcgccacc
     1441 gaccgcgacg agcatcgcaa atgccgacgt cccgagtggg cgaccgatga gcgagtcaag
     1501 aacgattccc ccaacgaagg cccacgtgat cccccggtcg atcccagcag caatggtcca
     1561 gatcaccccg ccgataagga cgaagtgcgg gccggcgttt ccactattga tgtacggcgc
     1621 gagcgtggct tcgacgagcg cgatgacggt tgccccgatc gcggcgagga ggaggctcat
     1681 gagacggcga tcggtcctcg gcgaacggag ggttcgcgcg cgaaattgag ccctcgcggc
     1741 gacgagcggt cggcacagcc gcgtcgttga ggaagtatcc cacggtgtcg cgaagagcgg
     1801 caatggtccg gatggaacgt gcggccacga cgttggtcgg gggcgatgtt gtgcgtctcg
     1861 ccactgtttc acgtgaaaca tcgcccacct ctcgccactg tttcacgtga aacctggccg
     1921 ggcgttggtg atccgggcgt aagcccgcgt aaaggggtgt gcgcgagact ccgacggagc
     1981 gtctcgccga cccgggaatc aaggagggaa aggcttcccg atgctagaat cgaccacagt
     2041 gaatgaacgc cggatgacca cctagagtgg gcgagacgat cgcctgtgcc aaccagaagg
     2101 gcggcgtcgg aaagaccacg acggttgtca atctggccag ctacctggcc ctcgcgggcg
     2161 accgcgtcct cgtggtcgac ctcgaccccc agggcaacgc cacgagcgcg tttggcatcg
     2221 accggacccg tgatcgatcc gtgtacgacg ccgtcatcga ccaagtccag ctcgaggagc
     2281 tggcgatcga cacggccgtt gacggcgtca cgctgattcc gtcatcgatc gcgctcgccg
     2341 gcgccgaggt tgagctgacg cccctcgagc agcgcgagcg ccgcctggcg acgctgctgg
     2401 atccgatgcg ggcgaagttc gactacctgc tgatcgactg cccgccatcg ctcgggttgc
     2461 tcaccatcaa cgcgttgacg gctgcggact cgaccctgat cccgatccag tgcgagtact
//
LOCUS       DATBSW010000093         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1241441, whole genome shotgun sequence.
ACCESSION   DATBSW010000093 DATBSW010000000
VERSION     DATBSW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241441"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1183)
                     /locus_tag="VIF63_02535"
     CDS             complement(146..1183)
                     /locus_tag="VIF63_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8238288.1"
                     /translation="MRNNSKRATGLVAAALFAISACSSPGATTGPTTAGATTVPATTG
                     PVALTGKITIDGSSTVYPITQAVGEEFNAANSGVEVAVGFAGTGGGFKKFCAGETDMN
                     DASRPIKTEDTPDAKSEGTLCKDAGIEYVELGIATDALSVVVSKDNTWATCMTTAQLK
                     TLWDVRPPEVTAGPDGSPPKTLWSDLDPTWPAEPVNLFGPGPDSGTFDYFTEVINGKA
                     KQSRSDYSPSEDDNALVTGVAGDKNALGYFGFSYLEENLDKIKAVAIDSGKGCVEPSS
                     ETVLGGTYTPLARPLFIYPSKAALARPEFVAFLNYYLENVNTFVDELGFIEATPETIT
                     KSKADLAAALP"
     gene            1335..2324
                     /locus_tag="VIF63_02540"
     CDS             1335..2324
                     /locus_tag="VIF63_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238289.1"
                     /translation="MHAAVMGGVPDWVRGERMSRRRVQAAIDIGSYSVHLLVAEVRGR
                     AVRALHDESAFLGLGREIDETGDLQAARPRLVETMSNFAMRAWSLGASSITVVATDPF
                     RRAPDAEAARADITAATGVDVNVLSHEEEALVALIGVQAGRAILRNTVVVDVGGGSTE
                     VLICGPAQEPVAAGLPLGAARLTGLLVKHDPPTQRELVALLGHSLTAMAAAPDVEQAE
                     LIAVGGTARSLLRVGPSLPNRTLTHRRVRHALDLMAAAPAEASAERFGIRLSRAHVLA
                     AGAAILLGALDRYRLDRLRVAAGGLREGMILSASRAGSEWRAELRSLARGWEA"
     gene            complement(2321..3565)
                     /locus_tag="VIF63_02545"
     CDS             complement(2321..3565)
                     /locus_tag="VIF63_02545"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY8238290.1"
                     /translation="MGLKPEADAQPEVDAQPEASKPSAATEPAPATKPAPRTEAAPTT
                     GEAALESEEPTARPLPSLGRAPGSIRPTDSFARAGRKAMWLHVSRMFAREEALRDPAE
                     TDALKRYRVATRRLRAALRIFRDAYPPRESKPLRNALALLADSLGVVRDLDVRIEALD
                     QWAAEQGNGAPKDIAPLREAWAAQRRAAAGALIRQLETKRHARLLDDLAAFVTADDSL
                     LSSPTEGPPRTIADRAASSVWAAYEQVRAYATVVRWADLEALHSLRIEAKRLRYVLEF
                     LGDVLGPERDAVIERLVALQDQLGALNDATIAVGAVRTFLGERHSTLRPAERSIVERY
                     LVEREREAGRLRRGLGRVWRPVVGVTFARRLGRAVVIRTVPRTATAVARAKVAARVRV
                     AARANVGTEVEAPAPRGDAAAT"
     gene            complement(3556..>4700)
                     /gene="ppk1"
                     /locus_tag="VIF63_02550"
     CDS             complement(3556..>4700)
                     /gene="ppk1"
                     /locus_tag="VIF63_02550"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY8238291.1"
                     /translation="PADVFAAMQAGDILVHHPYESFAASVERLVEQAADDPDVLTIKQ
                     TLYRTSLDSEVVNDLIRAAEQGKQVVVLVEVKARFDEQANIGWARKLERAGAHVVYGL
                     VGLKTHCKTLLIVRREGSMLRRYVHIGTGNYNTKTARSYVDLGLFTCREDIGSDVTDL
                     FNILTGLSRQRSFRRLLVAPTGLRDRFLELVEREIEHAGAGRPSGIVLKMNSLLDRAC
                     VEALYRASAAGVEVDLIIRGACTLVPGVAGLSERIRVRSIIGEFLEHSRIWRFNNGDL
                     PEWYIGSADLMDRNLDRRVEAFAPIDDPEAQAEIDEILRTTMADDRRSWILSDDGRWQ
                     RTERMTGVPGTIDSQQIARARAARRSHEAAAPHRAHAGLGSLEPWA"
BASE COUNT          639 a         1591 c         1718 g          752 t
ORIGIN      
        1 gacgcgctcg gacaatccgc gacgcagcac gagcggccgg ctggtggcgc tcactggctc
       61 ctcacttcac tcgaacaatg ggaagacgca ccgacccgat cgtcgaccgg gccggtgcgt
      121 gcgacaggac gttgtcggtt cgaccctacg gcagggcagc cgcgaggtcc gccttggact
      181 tcgtgatcgt ctcgggcgtg gcctcgatga agccgagctc gtcgacgaac gtgttgacgt
      241 tctcgaggta gtagttgagg aaggccacga actccggtcg agccagggcc gcctttgacg
      301 ggtagatgaa cagtggccgt gccagcggtg tgtaggtgcc gccgaggacc gtctccgagg
      361 agggctcgac gcaacccttg ccgctgtcga tcgcgaccgc cttgatcttg tcgaggttct
      421 cctcaaggta cgagaagccg aagtagccga gcgcgttctt gtcgccggcg acgccggtca
      481 cgagcgcgtt gtcgtcctcg gagggcgagt agtccgagcg ggactgcttt gccttgccgt
      541 tgatgacctc ggtgaagtag tcgaaggtgc cggagtccgg gccgggaccg aacaggttga
      601 caggctcggc cggccaggtc ggatcgaggt ccgaccacag ggtcttcggc gggctgccgt
      661 ccggaccggc ggtgacttcg ggcgggcgga cgtcccacag cgtcttgagc tgcgcggtcg
      721 tcatgcacgt tgcccaggtg ttgtccttgc tgacgacgac ggagagggcg tcggtggcga
      781 tgccgagctc gacgtactcg atgccggcgt ccttgcacag ggtgccttcg gacttcgcgt
      841 cgggcgtgtc ctccgtcttg atcggtcgag acgcgtcgtt catgtccgtc tcgccggcgc
      901 agaacttctt gaacccgccg ccggttccgg caaacccaac cgcgacctcg acgcccgaat
      961 tcgcggcgtt gaactcctcg ccgacggcct gggtgatcgg gtagaccgtg ctggagccgt
     1021 cgatggtgat cttgcccgtc agggcgaccg gcccggtcgt cgccgggacg gtggtcgcgc
     1081 cggcagtcgt cggcccggtc gttgcgccag ggctgctaca agcgctgatc gcgaacaagg
     1141 ccgccgcgac cagcccggtt gcacgctttg aattgttcct cacgcgatgc gttcctccaa
     1201 ttggaaggtg cctcgctcgg ggcgcaggca ctgtcgacgg atggttcccg tccgacggca
     1261 gtctcccgag cgagcgtcaa ccgttggtcg ctcgctcgtt aacattcgat gaacgagcgc
     1321 cgaacgagcg ccgcatgcat gcggccgtga tgggtggggt gccagactgg gttcgtggtg
     1381 agcgcatgag ccgccggcgt gtccaggcgg cgatcgacat cggctcctac tccgtccacc
     1441 tgctcgtggc ggaggtgcgc ggccgtgccg ttcgagccct acacgatgag tccgcgttcc
     1501 tcggcctggg ccgggagatc gatgagaccg gcgacctgca ggccgcgcga cctcgcctcg
     1561 tcgagacgat gtcgaacttc gccatgcggg cgtggtcgct cggcgcctcg tcgatcacgg
     1621 tcgtcgccac ggatccattc cggcgcgcac cggacgccga ggcggcccgg gccgacatca
     1681 ccgctgcgac cggcgtcgac gtgaatgtcc tcagccacga ggaggaggcg ctcgtcgcgc
     1741 tgatcggggt ccaggccggg cgggcgatcc tgcgcaacac cgtcgtcgtc gacgtgggcg
     1801 ggggcagcac ggaggtgctc atctgcggac cggcccagga gcccgtggcc gcgggactgc
     1861 cgctgggcgc tgcacgtctc acgggcctgc tggtcaagca cgatccgccg acacagcgcg
     1921 agctggtcgc gctcctcggg cattcgctca cggcgatggc ggccgcgccc gatgtcgagc
     1981 aggcggagct gatcgcggtt ggcgggacgg cgcgcagcct gctccgggtc ggcccgagct
     2041 tgccgaaccg gacgctcacc caccggcgag tccggcatgc gctcgacctg atggccgccg
     2101 cgcctgccga ggcgagcgcg gagcggttcg gcatccggct gtcgcgcgcc cacgtgctcg
     2161 cggctggcgc ggcgatcctg ctcggggcgt tggaccgata tcgcctggat cgtttgcggg
     2221 tggcggctgg cgggctgcgc gaaggcatga tcctgtcggc gtcgcgcgcc gggtcggaat
     2281 ggcgcgccga gctgcggtcg ctcgcgaggg gctgggaggc ctaggtcgcg gctgcgtcgc
     2341 cccgtggcgc cggcgcttcg acttctgtgc cgacgttggc tcgggccgcg accctgactc
     2401 gtgcggcgac cttggctcgg gcaaccgcgg tggccgtccg gggcaccgtc cggatgacga
     2461 cggcgcggcc gaggcggcgg gcgaacgtga ccccgacaac gggccgccag acgcggccga
     2521 ggcctcgccg caatcggccg gcctcccgtt cgcgctcgac gaggtaccgc tcgacgatcg
     2581 atcgctcggc gggccgcagc gtcgagtggc gctcgccgag gaacgtccga accgcgccga
     2641 cggcgatcgt cgcgtcgttc agcgccccga gctggtcctg gagggccacg agccgctcga
     2701 tgacggcatc ccgctccggc ccgagcacgt cgccgaggaa ctcgaggacg tagcgcaggc
     2761 gcttcgcctc gatccggagc gagtggagcg cttcgagatc tgcccaccga acgaccgtcg
     2821 cgtacgcccg gacctgctcg tacgcggccc agacgctcga tgcggcgcga tccgcgatgg
     2881 tccgcggcgg cccttccgtt ggcgagctga gcagcgagtc gtcggccgtg acgaatgcgg
     2941 cgaggtcgtc gagcaggcgc gcgtggcgct tggtctcgag ctggcgaatg agtgccccgg
     3001 ccgcggcacg gcgctgggcc gcccaggcct cgcgcaacgg cgcaatgtcc ttcggcgccc
     3061 cgttgccctg ctcggccgcc cactggtcca gcgcctcgat gcgcacatcg agatcccgga
     3121 ccacgccgag gctgtcggca aggagggcga gggcgttgcg cagcggcttc gactcacgcg
     3181 gcgggtaggc atcgcggaag attcggagcg ccgcgcgcag ccggcgagtc gcgacccggt
     3241 agcgcttcag ggcgtcggtc tcggcggggt cccggagcgc ttcctcgcgg gcgaacatcc
     3301 ggctgacgtg cagccacatc gcctttcggc cggccctggc aaacgagtca gtcggacgga
     3361 tcgaaccggg cgcccgaccg agcgacggca gcggtcgcgc ggtcggctct tcgctctcga
     3421 gcgccgcttc gccagtcgtt ggcgcggcct cggttcgggg tgccggcttg gtcgcgggcg
     3481 cgggctccgt tgcggccgac ggcttcgacg cctcgggttg cgcgtcgacc tcgggttgcg
     3541 cgtccgcctc cggtttcagg cccatggttc gagcgacccg agcccggcgt gggctcggtg
     3601 cggcgcggcc gcctcatgtg agcgccgggc agcgcgggct cgcgcgatct gctgcgagtc
     3661 gatcgtcccg ggcacgccgg tcatccgctc ggtgcgctgc cagcgtccgt cgtcggacag
     3721 gatccacgag cgccgatcgt cggccatcgt cgtgcgcagg atctcgtcga tctcggcctg
     3781 cgcctccggg tcgtcgatgg gcgcgaacgc ctcgacgcga cgatcgaggt tgcggtccat
     3841 gaggtcggcc gagccgatgt accactcggg cagatcgccg ttgttgaacc gccagatgcg
     3901 cgagtgctcg aggaactcgc cgatgatcga gcgcacgcgg atccgctccg acaggccggc
     3961 aacgccgggc acgagcgtgc acgccccgcg aatgatcagg tcgacctcga cgccggccgc
     4021 ggacgcgcga tacagcgcct cgacgcatgc ccggtcgagc agcgagttca tcttcagcac
     4081 gatccccgac ggccggcctg cgccggcgtg ctcgatctca cgctcgacga gctccaggaa
     4141 tcggtcgcgc agtccggtcg gtgcgacgag cagccggcgg aaggaccgct ggcgcgagag
     4201 cccggtcagg atgttgaaca ggtccgtgac atccgagccg atgtcctcgc ggcaagtgaa
     4261 cagcccgagg tcgacgtagg agcgcgcggt cttcgtgttg tagttgcccg tcccgatgtg
     4321 gacgtagcgc cggagcatcg agccctcgcg gcggacgatg agcagcgtct tgcagtgcgt
     4381 cttgaggccg acgagcccgt acacgacgtg cgcgccggcg cgttccagct tgcgcgccca
     4441 gccgatgttg gcctgctcgt cgaagcgggc cttgacctcg accaggacga ccacctgttt
     4501 gccctgctcg gcggcgcgga tgaggtcgtt gacgacctcg gagtcgagcg aggtgcggta
     4561 cagcgtctgc ttgatcgtca gcacgtccgg atcgtcggca gcctgctcga ccaggcgctc
     4621 gacgctcgcg gcgaagctct catacgggtg atggacgagg atgtcgcccg cctgcatcgc
     4681 ggcgaagacg tcagccggct
//
LOCUS       DATBSW010000094         8599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_677172, whole genome shotgun sequence.
ACCESSION   DATBSW010000094 DATBSW010000000
VERSION     DATBSW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677172"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VIF63_02555"
     CDS             complement(<1..957)
                     /locus_tag="VIF63_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY8238292.1"
                     /translation="MSDSKARPAHAALDWHPEPELISVRDAATSRDALRDAGAAIWET
                     ALDYLYDHALERAMGEHVEYDALRAAFFGPSGGPGPAPADPTTLQSVLDEFSARVAPH
                     TVSAYHPRSFGYFTPPPLLASVAGEVLAQVTQQGIDIWHAGPIGAFVEEEVVRWLCDV
                     VGYGDGSFGILTSGGVMANFIAMTLARDVHMPRLLGTDAPPRGRDLENVRVYTSDQTH
                     FSIGRALDELGFPPETLVVIPADASFRLRGAPVAEAITRDRARGLLPIAIAAVAGSTN
                     TGSVDAVGELADIAEREDLWLHVDAAYGAGARLSPRDASRVAD"
     gene            990..1358
                     /locus_tag="VIF63_02560"
     CDS             990..1358
                     /locus_tag="VIF63_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238293.1"
                     /translation="MPDTTPSNDDAPRPTVKPGVDYKGAPLDAEKGPGLGCFWLQVIV
                     LAIVVVLTPLSAAWEWPIPVTIVLLALTLVLLLFVGQTIIFLLRLVAADRRAEGRRRP
                     LSSETKTVGEIEDETPSVRE"
     gene            1412..1738
                     /locus_tag="VIF63_02565"
     CDS             1412..1738
                     /locus_tag="VIF63_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238294.1"
                     /translation="MPVLATYFTVRRGRDPFFKFYMKTLFPRQVREYGEKYGIKLIGW
                     YNVAHGWDFDNVILFDLPSYGVLDAMEQDEGIQAIAHRGAEWIMERHHTMFLRERMGP
                     ELKAHL"
     gene            1751..2662
                     /locus_tag="VIF63_02570"
     CDS             1751..2662
                     /locus_tag="VIF63_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238295.1"
                     /translation="MEARNALLAEMARDAELERTDFLARAGEQLDRFMAAHQHRIQQL
                     GGLVLIDDDPEYLAVAPDGTFRSRSRVFDETRGEWIAETEIIETAAELVEIYNPADIL
                     QSFAEAEAEDIGLGEGEAESLEGDVVEGDAGVVVDPYAEAADQWAAGQPEVREAHDES
                     GAALALYELALDYQDRSQRTEAGLVSSFEAAATGVLGYVGTLVIVDDDDEHLAIGAGG
                     FKGRVIAEGDTDWQDISSPDQIVRFYDPTDIFGDLAEALVDAYPAVATSSDAAEADDE
                     AEGDDDDDEADDDGDDDADAEPDNGKA"
     gene            2724..3584
                     /locus_tag="VIF63_02575"
     CDS             2724..3584
                     /locus_tag="VIF63_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY8238296.1"
                     /translation="MRLAGAELLDSREILPDQWLQTYHAPDLVAGARAGQFVHVRTGD
                     YSGLVLRRPFSFNTIDPATGNLTIHFRTIGRGTEWFTRLRPGDRVDMLGPLGRPFEVD
                     FRTQHLLLIAGGLGIAGVRSLADEAIRDGRQVTLLFGGASARDVYPSSLLPDEVEYVV
                     ATDDGSVGHHGFVTELVANYDGWADQAFACGPLPMLKAVARLAASRRERLGVAKLGRK
                     RGGGKVVPSGSPQARRKSFLQVSMEQNMGCAVGACLGCVVMSTSGTPQRVCREGPVFA
                     SDELDWEGGW"
     gene            3581..4804
                     /locus_tag="VIF63_02580"
     CDS             3581..4804
                     /locus_tag="VIF63_02580"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY8238297.1"
                     /translation="MKAKRRVVTSGKGGGALGKGSASTRGGAAGRGTPKRRQTVRKIK
                     PAILPVTRRPALPALADTVPTSPRSLGSANVTVQATNGHLPPDAAVDLSVDLGRGLVL
                     ENPILVASGTFGYGTEYSDVVEIQRLGAICCKGTTLRPRVGNPTPRVTETPGGMLNSI
                     GLQNPGVDAVIAKYAGTWANWRVPVIVNVAGESIADYVEVARRLEGVPGIAGIELNIS
                     CPNVGAGGIQFAIDREAAAAVTSAVRKATDLPLLVKLSPNVADVRPIARAIADAGADA
                     LTAINTLSGIAVAPTRGRPLLGNIYGGLSGPAIKPVALRIVYEVSQVVDIPVVAIGGV
                     TELADVLDFLAVGAAAVQVGTAIFADPTLPVRLVDELAAECRRRGFSSFAPLIGTALP
                     KKASAPSSKGVEYRP"
     gene            4889..5839
                     /locus_tag="VIF63_02585"
     CDS             4889..5839
                     /locus_tag="VIF63_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY8238298.1"
                     /translation="MHSTPTGERSWFVPAAIVAGVLGSFVVLVMLGSQVSGILSTVGS
                     SVGGDSIGSDPDAGSGGDTSGGSGGDSTAPGVVTASARDGALIIKTGAMSLQVAAIDA
                     AVTAATQRIDALGGYASGSQRAGTGDEAEATITFRVPAERWDDAMNGVRALADQVLSE
                     GSTTEDVTTQVVDLGARIRNLQATEQALQAIMDRAAVIEDVLSVQAELTTVRGTIEQL
                     TAQKAHLEAQAALSTLTVTFAVRPIPVLVEQRAQFDPAGEVDAASATFVSILQRLVKA
                     GIWFAIVWLPSLLGLAIGAVIAFLVIRRLRKLLPIPAREG"
     gene            6035..6916
                     /locus_tag="VIF63_02590"
     CDS             6035..6916
                     /locus_tag="VIF63_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY8238299.1"
                     /translation="MIAAACGGAGAALAPVPGAPQAGGEQSGGGQPLSGNGDGDGTDR
                     GVLYAAGQPNLLIIKNGSLVLQVTGIDAALTSATQQISALNGYVSGSERSGDGEFDQA
                     TLTFRIPAQSWDDALVRLRALGTKILDERSTTQDVTSQVVDLGARLKNLQATELALQG
                     TMAKAVEIKDILAVQAELTTVRGQIEQIAAQKTSLEQQAAYSTLAVTFSLKPNPVLTS
                     TTQFDPNSEVEQASASLVSFLQSLATAGIWFAIVWVPILIVLAIIVGIGAFVYRRYRR
                     SDPVGAAPVAPTGDSAS"
     gene            6981..7655
                     /gene="pyrE"
                     /locus_tag="VIF63_02595"
     CDS             6981..7655
                     /gene="pyrE"
                     /locus_tag="VIF63_02595"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY8238300.1"
                     /translation="MTTIKHGVELAARAAIARRTEQLFRSSGALRDGHFHLKSGRHSE
                     AYLEKFAVLSDPAATSELCGFWAGQHRGPDDRPTVDLVAGPTTGGVILAFETGRQLGT
                     RAIFAEEIKGEVPPRREFRRGFRIEPGERVLLVDDILTTGGSLLAMIPAVEAAGGEIV
                     ACAVMVDRSGGMQSLESPTTGRRYPLSALWILDLPTYEAGPATCPACAAGTPIYAPGS
                     TGAATA"
     gene            7652..8071
                     /locus_tag="VIF63_02600"
     CDS             7652..8071
                     /locus_tag="VIF63_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238301.1"
                     /translation="MSDRQPDELPKKDHGGLRRARFLPVLGILVAVGVAAVLLGILAP
                     RVACACSPTVPPLPPSPMVGVVIAVESAGVGQVQGFTLRLPDAVSYTFTLGTLENATE
                     FSPSHLAEHQITSAPIRAFYRVENGVLVAYRLEDAPN"
     gene            complement(8081..>8599)
                     /locus_tag="VIF63_02605"
     CDS             complement(8081..>8599)
                     /locus_tag="VIF63_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238302.1"
                     /translation="GAYSTVGSTWTSTSFSTTVTVGHTYTFRVRARDWAGNVGAWKVA
                     AIVKPAVTQQTSSHVTYSGTWSTGSTSKYTGGTVKYATAAGASATYSFTGRSIAWVTT
                     KATRSGSARIYIDGVLASTMSLYASSYTYKYVAFEMSWTTSGAHTIKVEVVGTSGHPR
                     IDIDAFLVLVNG"
BASE COUNT         1292 a         3048 c         2994 g         1265 t
ORIGIN      
        1 gtccgcgacc cggctcgcgt cacgcggcga gaggcgtgcc ccggcgccgt acgccgcgtc
       61 cacgtgcagc cacaggtcct cgcgctcggc gatgtcggcg agctcgccga ccgcgtcgac
      121 ggagccggta ttcgtcgatc ccgcgaccgc ggcgatcgcg atcggcagca ggcctcgtgc
      181 ccggtcgcgc gtgatcgcct cggcgaccgg cgcgccccgc aagcggaacg acgcatccgc
      241 cgggatgacg acgagcgtct ccggcgggaa gccgagctcg tcgagcgcgc ggccgatcga
      301 aaaatgggtc tggtcggacg tgtagacgcg gacgttctcg aggtcgcgac cccgtggcgg
      361 ggcgtccgtc ccgagcaggc ggggcatgtg gacgtcgcgg gcgagggtca tcgcgatgaa
      421 gttggccatc acgccgcccg aggtcaggat cccgaagctg ccgtcgccat agccgacgac
      481 gtcgcacagc cagcgaacga cctcttcctc gacgaacgcg ccgatcgggc cggcgtgcca
      541 gatgtcgatg ccctgctggg tcacctgggc gagcacttcg ccggccaccg acgcaagcag
      601 cggcggcggc gtgaagtagc cgaacgagcg cgggtgataa gcgctcacgg tgtgcggcgc
      661 gacgcgggcc gagaactcgt cgaggaccga ctggagggtc gtcgggtcgg ccggcgccgg
      721 cccggggccg cccgacggcc cgaagaacgc ggcgcgcagc gcgtcgtact cgacgtgctc
      781 gcccatcgcc cgctcgagcg cgtggtcgta gaggtagtcg agcgcggtct cccagatggc
      841 cgcgccggcg tcccggaggg cgtcgcgcga cgtcgccgcg tcgcgcaccg agatcagctc
      901 gggctcggga tgccagtcga gtgcggcgtg agcggggcgc gccttcgagt cggacaccca
      961 aagatggtac cggccgcgat aattgccccg tgccagacac cacgccgtcg aacgacgacg
     1021 ccccgcgacc gaccgtcaag cccggcgtcg actacaaggg cgccccgctc gatgccgaga
     1081 aggggccggg ccttggctgc ttctggctcc aggtcatcgt gctcgcgatc gtggtcgtcc
     1141 tgaccccgct gagcgccgcc tgggaatggc cgatcccggt aacgatcgtc ctgctcgcgc
     1201 tgacgctcgt gctcctgctg ttcgtcggcc agacgatcat cttcctgctc cgcctggtcg
     1261 ccgccgatcg gcgggccgag ggtcgccgtc gcccgctcag ctccgagacc aagaccgtcg
     1321 gcgagatcga ggacgagacg ccaagcgtgc gagaataggc cgccccgaac cggagaccgg
     1381 ttcaaccagc gcctggagga ccctcgcgac cgtgcccgtg ctcgccacgt acttcaccgt
     1441 ccgccgcggt cgcgacccgt tcttcaagtt ctacatgaag acgctcttcc cgcgacaggt
     1501 tcgcgagtac ggcgagaagt acgggatcaa gctcatcggc tggtacaacg tggcccacgg
     1561 ctgggacttc gacaacgtga tcctgttcga cctgccgagc tacggtgtcc tcgacgcgat
     1621 ggagcaggac gaagggatcc aggcgattgc ccaccgcggc gccgaatgga tcatggagcg
     1681 ccaccacacg atgttcctcc gtgagcggat gggacccgag ctcaaagccc acctgtagga
     1741 ggaatcggac atggaagcgc gaaacgccct cctggccgag atggctcggg acgcagagct
     1801 cgagcggacg gacttcctcg cccgcgcggg cgagcagctc gaccggttca tggcggccca
     1861 ccagcaccgg atccagcagc tcggcggcct cgtcctgatc gacgacgacc ccgagtacct
     1921 cgccgtcgcg cccgacggca cgttccgcag ccgcagccgc gtcttcgacg agacgcgggg
     1981 cgagtggatc gccgagacgg agatcatcga gacggccgcg gagctggtcg agatctacaa
     2041 cccggcggac atcctccagt cgttcgccga ggccgaggcc gaggacatcg gcctgggcga
     2101 gggcgaggcc gagagcctcg agggcgacgt tgtcgagggc gacgccggcg tcgtcgtgga
     2161 tccctacgcc gaggccgccg accagtgggc tgccggccag cccgaggtcc gcgaggccca
     2221 cgacgagagt ggcgccgccc tcgccctgta cgagctcgcg ctcgactacc aggaccgcag
     2281 ccagcgcacc gaggccggcc tcgtcagctc gttcgaggct gccgccaccg gtgtcctcgg
     2341 ctacgtcggg acgctcgtga tcgtcgatga cgacgacgag cacctcgcga tcggcgcggg
     2401 cgggttcaag ggccgcgtga tcgccgaggg cgacaccgac tggcaggaca tctccagccc
     2461 ggaccagatc gtccgcttct acgacccgac cgacatcttc ggcgacctcg ccgaggccct
     2521 cgtcgacgca tacccggccg tcgccacctc gagtgacgct gccgaggcgg acgacgaagc
     2581 cgaaggcgat gacgacgacg acgaggccga tgacgacggc gacgatgacg ccgacgccga
     2641 accggacaac ggcaaggcct agggcccgaa ccctggccac ccgcgctcga gacccgagcc
     2701 cagcgcgccc ggcagtcgcg tccatgcgac tggccggtgc ggagctgctc gacagtcgtg
     2761 agatcctgcc cgaccaatgg ctccagacct accacgcgcc tgacctcgtc gcgggtgctc
     2821 gggcgggcca gttcgtccac gttcggacgg gcgactactc ggggctcgtg ctgcgccggc
     2881 cgttctcgtt caacacgatc gacccggcca cgggcaacct cacgatccat ttccgaacga
     2941 tcgggcgcgg gaccgagtgg ttcacccggc ttcgaccggg tgatcgcgtc gacatgcttg
     3001 gtcctctggg ccggccgttc gaagtcgatt tccggaccca gcaccttctg ctgatcgccg
     3061 gcggcctcgg cattgccggg gtccgatcgc tcgccgacga ggcgatccgc gacggccgcc
     3121 aggtcacgct gctgttcggt ggcgcgagcg cgcgggacgt ctatccgtcg tcgctcctgc
     3181 ccgacgaggt ggagtacgtc gtcgccaccg atgacggctc ggtggggcac cacggcttcg
     3241 tcaccgagct cgtcgccaac tacgacggct gggccgacca ggccttcgcc tgcggcccgc
     3301 tgccgatgct gaaggccgtg gcccggctgg ctgcgtcgcg ccgggagcgg ctcggggtcg
     3361 cgaagctggg tcggaagcgc ggcggcggga aggtcgtgcc gtcgggctcg ccgcaggcgc
     3421 gacggaagtc gttcctccag gtgtcgatgg agcagaacat gggctgcgcg gtcggggcat
     3481 gcctgggctg cgtcgtcatg tcgacgtcgg gcacgccgca gcgcgtctgc cgcgaaggcc
     3541 cggtcttcgc ctcggacgag ctcgattggg agggcggctg gtgaaggcca agcgccgcgt
     3601 cgtgacctcg ggcaagggcg gcggagctct cggtaagggc agcgcctcga caagaggcgg
     3661 cgctgcgggc aggggcacgc ccaagcgtcg ccagacggtg cgcaagatca agccagcgat
     3721 cctgccggtg acccgccgcc cggcgctgcc ggcgctcgcc gacaccgtgc cgacgagccc
     3781 tcgctcgctc ggcagcgcga acgtcaccgt ccaggcgacg aacgggcacc tgccacccga
     3841 tgccgccgtc gacctgtccg tcgatctcgg gcggggtctc gtcctcgaaa acccgatcct
     3901 cgtcgcgtcg gggacgttcg gctacggcac cgagtacagc gatgtcgtcg agatccagcg
     3961 gctcggtgcg atctgctgca agggcacgac gctgcggcca cgcgtcggca acccgacgcc
     4021 gcgcgtgacc gagacgcccg gcgggatgct caactcgatc gggctccaga accccggtgt
     4081 cgacgccgtg atcgcgaagt acgccgggac gtgggcgaac tggcgcgtgc cggtgatcgt
     4141 caacgtcgcg ggtgagtcga tcgcggacta cgtcgaggtc gcccgccggc tcgagggcgt
     4201 gccgggcatc gccgggatcg agctgaacat cagctgcccg aacgtcggcg ccggagggat
     4261 ccagttcgcc atcgatcgcg aggcggcggc tgccgtgacc tccgcggtgc gcaaggcgac
     4321 cgacctgccc ctgctcgtga agctgtcgcc gaatgtcgcc gacgtccggc ccatcgcccg
     4381 cgccatcgcg gacgccggcg ccgacgcgct gacggcgatc aacacgctct ccgggattgc
     4441 cgtcgcgccg actcgcggtc ggccgctgct gggcaacatc tacggtggcc tttcgggtcc
     4501 cgcgatcaag cccgtcgcgc tccgcatcgt ctacgaggtc agccaggtcg tggacatccc
     4561 ggtcgtcgcg atcgggggcg tgacggagct cgccgacgtg ctcgatttcc tcgctgtcgg
     4621 cgcggcggcg gtccaggtcg gcacggcgat cttcgccgac ccgaccctgc cggtccgcct
     4681 cgtcgacgag ctcgccgccg aatgccgccg ccgcggcttc tcgagcttcg cgccgctcat
     4741 cggcacggcg ctgccgaaga aggcgtccgc cccatcctcg aagggtgtcg agtaccgccc
     4801 gtagcgctgg gattgcggag gatcccgcag tccgctcgag ggtccgcacc gatagtccga
     4861 ctgttcgacg gctgccagcg ttgcgggcat gcattcgacg ccgaccggtg agcgctcctg
     4921 gttcgtgccc gcggccatcg ttgccggggt gctggggagc ttcgtggtcc tggtcatgct
     4981 cggctcgcag gtgagcggca ttctctcgac ggtcggctcg tccgtcggcg gcgactccat
     5041 cggatccgac cccgacgcgg gttcaggggg cgacacgtcc ggcgggtcag gtggcgactc
     5101 caccgcaccg ggcgtcgtga cggcgagcgc ccgcgatggc gccttgatca tcaagaccgg
     5161 ggcgatgtcc ctccaggtcg ccgcgatcga cgccgcagtg acggccgcaa cgcagcgaat
     5221 cgacgcgctt ggcggctacg ccagcgggtc gcagcgagcc gggaccggcg atgaagcgga
     5281 ggcgacgatc acgttccggg tgccggcgga gcggtgggac gacgcgatga acggtgttcg
     5341 cgccctcgcc gaccaggtgc tctcggaggg atcgaccacg gaggatgtga cgacgcaggt
     5401 ggtcgatctc ggcgcccgca tccgcaacct ccaggccacc gagcaggctc tccaggcgat
     5461 catggatcgc gccgctgtga tcgaggacgt cctgtccgtg caagccgagc tcacgacggt
     5521 gcgcggcacc atcgagcagc tcacggccca gaaggcgcac ctcgaggcgc aagcggcact
     5581 ctcgacgctg acggtcacat tcgccgttcg gccgataccc gtgctcgtcg agcagcgagc
     5641 ccagttcgat cctgcaggcg aggttgatgc cgcctcggca acgttcgtga gcatcctcca
     5701 gcgactcgtg aaggcaggca tctggttcgc gatcgtctgg ctgccgagcc tgctcgggct
     5761 cgcgatcggc gccgtcatcg ccttcctggt catccgccgt ctgcggaagc tcctgcccat
     5821 tcctgctcgc gagggctgaa tcccggtagc gcggtcccag gccctgaatg aaatcttcac
     5881 ctccggcgcg gaaccattcg ggtagcgtga ccgtcgtcag gcagcccgat cgcctgcgtg
     5941 ctgatcgagc tttgagagga gcagcgtgat gctgtccatg gcccgaacgg tcggccgacg
     6001 gggttcgacc cccctcgtga ttctcctggt cgcgatgatc gccgcggcat gcggcggcgc
     6061 cggggccgcc ctggcgccgg tcccgggagc gccccaggcg ggtggcgagc aatctggagg
     6121 tgggcagccg ctgagcggca acggcgacgg cgacggcacc gaccgcggcg tcctgtatgc
     6181 cgccggccag ccgaacctgc tgatcatcaa gaacggttcg ctcgtcctgc aggtgaccgg
     6241 catcgacgcc gccctgacct cggcaacgca gcagatctcg gcgctcaacg gctacgtcag
     6301 tggctcggag cgctccggcg atggcgagtt cgaccaggcg accctcacct tccgcattcc
     6361 ggcgcagagc tgggacgacg ccctggtccg cctccgggcc ctcgggacga agatcctcga
     6421 cgagcggtcg acgacccagg acgtcacctc gcaggtcgtc gacctcgggg cgcgcctcaa
     6481 gaacctgcag gcgaccgagc tggcgctcca ggggaccatg gcgaaggccg tcgagatcaa
     6541 ggacatcctc gcggtccagg ccgagctgac cacggtccgc ggccagatcg agcagatcgc
     6601 cgcgcagaag accagtctcg agcagcaggc cgcgtactcg acgctcgccg tcacgttcag
     6661 cctcaagccg aacccggtgc tgacgtccac cacgcagttc gacccgaact ccgaggtcga
     6721 gcaggcgtcg gccagcctcg tcagcttcct ccagtcgctc gcgaccgcgg gcatctggtt
     6781 cgcgatcgtg tgggtgccga tcctgatcgt gctcgcgatc atcgtcggca tcggagcctt
     6841 cgtgtaccgc cggtaccgcc ggtccgaccc ggtcggtgcc gctcccgttg ctccgaccgg
     6901 ggacagcgcc tcgtaggcag gttcgacagg gagatcgggc ggacgggtat ctccgccgcc
     6961 cgacctacac tccggggcgc atgaccacga tcaagcacgg cgtcgagctg gccgcccggg
     7021 ccgcgatcgc gcggcgcacg gagcagctct tccgcagctc cggcgcgctc cgcgacggcc
     7081 acttccactt gaagagcggg cgccattcgg aggcgtacct cgagaagttc gccgtcctgt
     7141 cggacccggc cgcgaccagc gagctgtgcg gcttctgggc cggccagcat cgcgggccgg
     7201 atgatcgacc gacggtcgac ctcgtcgccg ggccgaccac cggcggcgtc atcctggcgt
     7261 tcgagacggg ccggcagctc gggacgcggg cgatctttgc cgaggagatc aagggcgagg
     7321 tcccgccccg gcgcgagttc cgccgcggct tccggatcga gcccggtgag cgagtcctgc
     7381 tcgtcgacga catcctcacc accggtggct cgctgctcgc gatgatcccg gcggtcgagg
     7441 cggcgggcgg ggagatcgtc gcgtgcgcgg tgatggtcga ccggtcgggc ggcatgcagt
     7501 cgctcgaatc gcccacgacc gggcgccgat atccgttgag cgccctctgg atcctcgacc
     7561 tgcccacgta cgaggcgggt ccggcaacct gcccggcgtg cgccgccggc acgcccatct
     7621 acgcgcccgg cagcacggga gccgccacgg cgtgagcgac cgccaacccg acgagcttcc
     7681 gaagaaggat cacggcggtc tgcgccgggc acggttcctg ccggtgctgg gcatcctcgt
     7741 agccgtgggc gtggctgcgg tgctcctcgg aatcctggcg ccccgcgtcg cgtgcgcctg
     7801 cagccccacc gtgccgccgc tcccgccgtc gccgatggtc ggcgtcgtca tcgcggtcga
     7861 gtcggcgggc gtcggccagg tccagggttt caccctccgg ttgcccgatg ccgtcagcta
     7921 cacgttcacg ctcggcaccc tcgagaacgc caccgagttc tcgccgagcc acctcgccga
     7981 gcaccagatc acgagcgcgc caattcgagc cttctatcga gtcgagaacg gcgtcctggt
     8041 cgcctaccgc ctcgaggacg cgcccaactg acggcgacgg ctacccgttc acgaggacca
     8101 ggaacgcgtc gatgtcgatc cgggggtggc ctgacgtgcc gacaacctcg accttgatcg
     8161 tgtgggcgcc ggatgtcgtc caggacatct cgaacgcgac gtacttgtac gtgtaggaag
     8221 acgcgtagag gctcatggtc gaggcgagga ccccgtcgat gtagatccgc gccgacccgc
     8281 ttcgggtcgc cttggtcgtc acccacgcga tcgaacgacc ggtgaaggaa tacgtggcgc
     8341 tggcgcccgc cgcggtcgcg tacttcaccg ttccgccggt gtacttgctc gtcgatccgg
     8401 tcgaccaggt gccggaatac gtcacatgtg acgaggtctg ctgcgtgacc gcgggcttga
     8461 cgatcgccgc gaccttccac gctccgacgt tgccggccca gtcgcgagcc cggacccgga
     8521 acgtgtacgt gtgcccgacg gtgaccgttg tgctgaacga cgtcgatgtc cacgtcgacc
     8581 caacggtcga atacgcgcc
//
LOCUS       DATBSW010000095         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_289441, whole genome shotgun sequence.
ACCESSION   DATBSW010000095 DATBSW010000000
VERSION     DATBSW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289441"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VIF63_02610"
     CDS             <1..284
                     /locus_tag="VIF63_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HEY8238303.1"
                     /translation="LVIEVVDRLGDRDGPAAGRFRLAASRGSATCARTDAQPGLTLDA
                     KALGAIYLGGTRLVDVTRAGGATEHRAGVLAEVDRLFRTADEPWCSTWF"
     gene            complement(332..745)
                     /gene="msrB"
                     /locus_tag="VIF63_02615"
     CDS             complement(332..745)
                     /gene="msrB"
                     /locus_tag="VIF63_02615"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY8238304.1"
                     /translation="MDTTDLEARLGKLSPIQYAVTQQGQTERAFTGVYWDHHGDGAYR
                     CVVCETPLFDSGTKFESGTGWPSFFDEIEPGRVKTIEDRSFGMRRIEAVCANCGAHLG
                     HVFDDGPRPTGLRYCMNSASLEFDSHGGAAPEGQS"
     gene            complement(805..1407)
                     /locus_tag="VIF63_02620"
     CDS             complement(805..1407)
                     /locus_tag="VIF63_02620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8238305.1"
                     /translation="MTGGSWDDSYLHNRTPWDIGRPQPAIARLADVRELIEPVLDVGC
                     GSGEHALLAAIGGLEVTGVDISQLAVERARAKARQRGLSLEFLVGDVLALEKIERLHP
                     PFRTVLDVGCFHTFANADRPLYASSLADVVESGGVLHLLCFSELTPGTDGPRRVTQAE
                     IAQTFSRDWYVDRIEPDRFAVSTLWSGPEPHAWLARIVRR"
     gene            complement(1474..1836)
                     /locus_tag="VIF63_02625"
     CDS             complement(1474..1836)
                     /locus_tag="VIF63_02625"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8238306.1"
                     /translation="MQTDPRVDAYLAGLPAEQRIVLQALRERVAALVPEAEDTISYSM
                     PAFKLGNRFLLSYAGWKQHCSVYPFGDELAARYADRLEGYGRTKGSLHFSAAQPLPDG
                     LLDELVRDRVATVEADGR"
     gene            complement(1908..2462)
                     /locus_tag="VIF63_02630"
     CDS             complement(1908..2462)
                     /locus_tag="VIF63_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238307.1"
                     /translation="MRRHICAVLLVALLVAACGSSTASAPPRPTAPAGWTVVVSDSQD
                     VRLTLPPWMVAWDTRSGAIFANEAPLGPGADFLELMATGPQADDQPGPTERLEDWLEA
                     KLAPAGRGVGNAQSIVLPAGRGIVIDRVVEVGTPNIWRFAAYAIRTPAGVGYLLLDGP
                     PNLWAVHEADMNLIPLLMELGPGR"
     gene            complement(2498..2572)
                     /locus_tag="VIF63_02635"
     tRNA            complement(2498..2572)
                     /locus_tag="VIF63_02635"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2538..2540),aa:Asn,seq:gtt)
     gene            2720..3340
                     /locus_tag="VIF63_02640"
     CDS             2720..3340
                     /locus_tag="VIF63_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238308.1"
                     /translation="MDARQRVGVVLVVAGVALALVGAGGAVGVFGGPAAATGSPTPAP
                     NPTAAAIATAPAMTVAAPTVVPATVAPPTAIPTEDTLAIVTAFFAELEHAVQIGAQET
                     MTDNLGQATIDRYGRDECSTNLAIKEPVPEQAFEILALSPPAAWDYVTDELTTTVPDA
                     ITVQARVTGPDATGGITTAERELHIQVLDGIVYWFTDCGTPLVPTP"
     gene            complement(3344..4120)
                     /locus_tag="VIF63_02645"
     CDS             complement(3344..4120)
                     /locus_tag="VIF63_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY8238309.1"
                     /translation="MTYRTRLAIGLCLLTLVATLGACSAAAGPQLADRVFLSVAVTDG
                     GAPRALVPGTRIRLTFQANNLGASAGCNMMGGTYRIDGGVLVTDALAMTEMGCDDERH
                     AQDDWLSALLGSRPTIRLNGDELTLESGSVVVRLMDRKVVEPDLALVGPTWTVDSIIS
                     GDAISSVPAGAVATIVFAADGTVTVKPGCNEGQGTWKSVGTGIEISGIGLTKKACQGP
                     AGQLETAVMAVLGAGQLASTIDANLLTLQSGANGLQLRGS"
BASE COUNT          640 a         1471 c         1597 g          631 t
ORIGIN      
        1 acctggtgat cgaggtcgtc gaccgcctgg gtgatcggga cggcccggcg gccggccgat
       61 tccgcctggc ggcgagccgc ggctctgcga cgtgcgcccg aaccgacgcc cagcccggcc
      121 tcaccctcga cgcaaaggcc ctcggcgcga tctacctcgg cggcactcgc ctggtcgatg
      181 tcacgcgggc gggcggggcg accgagcacc gggcgggtgt gctggccgag gtcgatcggc
      241 tgttccgaac cgccgacgaa ccgtggtgct cgacctggtt ctgagctcgt gctgagctcg
      301 ttctgagctc gtcgcaggct cgttctgtgt tttagctctg gccctcgggc gcggccccgc
      361 cgtgcgagtc gaactccagc gaggccgagt tcatgcagta gcgcaggccg gtcggccgcg
      421 ggccgtcgtc gaagacgtgg cccaggtgcg cgccgcagtt cgcgcagacg gcctcgatcc
      481 gacgcatgcc gaaggaccgg tcctcgatcg tcttcacccg gcccggctcg atctcgtcga
      541 agaagctcgg ccagcccgtg ccggactcga acttcgtgcc ggagtcgaag agcggggtct
      601 cgcacacgac gcagcggtag gcgccgtcgc cgtggtggtc ccagtagacg ccggtgaacg
      661 cacgctcggt ctggccctgc tgggtgaccg cgtactggat ggggctgagc ttgccgaggc
      721 gagcctcgag atcggtggtg tccatgggcc gaatcctacg caggatcgat gtcgcgacgg
      781 tgacggaggc cttgaccgca ccgctcagcg gcggacgatc ctcgccagcc acgcgtgcgg
      841 ctcggggccc gaccagaggg tcgacacggc gaacctgtcg ggctcgatgc ggtcgacgta
      901 ccagtcgcgc gagaacgtct gcgcgatctc ggcctgggtg acgcggcgcg ggccgtccgt
      961 gccgggcgtc agctcggaga agcacagcag gtggaggacg ccgcccgact cgaccacgtc
     1021 ggcgaggctc gatgcgtaga gcgggcggtc ggcgttggcg aaggtgtgga agcagccgac
     1081 gtcgagcacg gtgcggaacg gcggatggag gcgctcgatc ttctcgaggg cgagcacatc
     1141 gccgacgagg aattcgagcg acagcccgcg ctggcgggcc ttcgcccgcg cccgctccac
     1201 ggcgagctgc gagatgtcga caccggtgac ctccaggcca ccgatggccg cgaggagggc
     1261 gtgctcaccg gagccgcacc cgacgtcgag caccggctcg atcagctcgc ggacatccgc
     1321 caggcgcgcg atcgccggct gtgggcgacc gatgtcccac ggcgtccggt tgtgcaggta
     1381 gctgtcgtcc cacgaccccc cggtcatggc cgaaggatac gggtcgaggg aatgccggga
     1441 cggttcatgg acgaatggct ggtgcggccg tgcctaccga ccgtcggctt cgacggtcgc
     1501 gacgcggtca cggacgagct cgtcgagcag cccgtcgggc agcggctggg ccgcgctgaa
     1561 atgcaggctc cccttcgtcc ggccgtagcc ctccagtcgg tcggcgtacc gagcggccag
     1621 ctcgtcgccg aacgggtaca cgctgcagtg ctgcttccag cctgcgtacg acaggaggaa
     1681 ccgattcccg agcttgaagg ccggcatcga gtagctgatg gtgtcctcgg cctccggcac
     1741 cagggcggca acgcgctcgc ggagtgcctg gaggacgatc cgttgctcgg cggggaggcc
     1801 ggcgaggtag gcatcgacgc gtgggtcggt ctgcatgtcc cacggattct gtaccgtctg
     1861 ccggctcgcg tcgaatgcgt tcacggaatc ggtcgcgggg aatggcgtca gcggccgggg
     1921 ccgagctcca tgagcagcgg gatcaggttc atgtcggcct cgtggacggc ccacaggttg
     1981 ggcgggccgt cgagcaggag gtagccgacg ccggcgggcg ttcggatggc gtaggcggcg
     2041 aagcgccaga tgttcggggt gccgacctcc accacgcggt cgatgacgat gccacggccg
     2101 gctgggagca cgatgctctg cgcgttgccg acgccccggc cagccggcgc aagcttcgcc
     2161 tcgagccagt cctcgagccg ctccgtgggg cctggctggt cgtcggcctg gggaccggtc
     2221 gccatgagct cgaggaagtc ggcccccggt ccgagcggcg cctcgttcgc gaagatcgcg
     2281 ccgctacggg tatcccaggc gaccatccag ggtggcaacg tcagacgcac gtcctgtgaa
     2341 tcgctgacga cgaccgtcca gcctgcgggc gcggtcggtc gaggcggcgc tgaggcagtc
     2401 gacgagccgc aggcagccac gaggagtgcg accagcagca cggcgcagat atgtcgtctc
     2461 acggtatagc ggaacaggat acatggtcgg gaaaagctgg ctccggcggt aggattcgaa
     2521 cctacgacat tgcggttaac agccgcctgc tctaccactg agctacgccg gagcggggcc
     2581 gccgtgagcg acctgcgggc acgccgagcc gatccgtcgg atcgggggtg ccgcgccgca
     2641 cgggaggata ccagaggcct cgcgcgcggc gatctccgag gctatgcgag cgtcgagact
     2701 gacgctatcg tcggcgctga tggacgcgcg ccagcgggtc ggggtggtgc tcgtggtcgc
     2761 gggtgtcgcg cttgcgctcg tcggagccgg cggcgcggtc ggggtcttcg gcgggccggc
     2821 cgccgcgacc ggctccccca ctccggcgcc gaatcccacg gccgcggcga tcgcgaccgc
     2881 gccggcaatg accgtcgccg cgcccaccgt cgtcccggcc acggtcgctc caccgacggc
     2941 gatcccaacc gaggacaccc tcgcgatcgt gaccgcgttc ttcgcggagc tcgagcacgc
     3001 cgtccagatc ggcgcgcagg agacgatgac cgacaacctc ggccaggcga cgatcgaccg
     3061 ctacggccgc gatgagtgct ccacgaacct ggcgatcaag gagcccgtgc ccgagcaggc
     3121 gttcgagatc ctggccctca gcccgcccgc tgcgtgggac tacgtgaccg acgagctcac
     3181 gacgaccgtc cccgacgcga tcacggtcca ggcgcgagtg acgggcccgg atgcgaccgg
     3241 tggcatcacg accgcggagc gcgagctcca catccaggtg ctcgacggga tcgtgtactg
     3301 gttcaccgac tgcgggacgc ccctcgtccc gacgccgtaa ggcctacgac ccgcgcagct
     3361 ggagcccgtt ggcacctgac tgcagcgtca ggagattggc gtcgatcgtg gacgccagct
     3421 ggcccgcccc gagcacggcc atcactgccg tttcgagctg cccggccggg ccctggcacg
     3481 ccttcttcgt gaggccgatg ccggagatct cgatgccggt gccgaccgac ttccacgtgc
     3541 cctggccttc gttgcagccc ggcttgaccg tgacggtgcc gtcggcggcg aagacgatcg
     3601 tcgcaaccgc gccggccggg acgctcgata tcgcgtcgcc gctgatgatc gagtcgaccg
     3661 tccaggtcgg cccgacgagg gccaggtccg gctcgacgac cttgcgatcc atcagccgga
     3721 cgacgacgga gcccgattcg agcgtcagct cgtcgccgtt gaggcggatc gttggcctcg
     3781 aaccgaggag cgcggagagc cagtcgtcct gggcgtggcg ctcgtcgtcg cagcccatct
     3841 cggtcatcgc gagcgcgtcg gtgacgagga cgccaccgtc gatccgatag gtcccgccca
     3901 tcatgttgca gccggcggat gcgccgaggt tgtttgcctg gaaggtgagg cggatgcggg
     3961 tgcccgggac gagggcgcga ggcgccccgc cgtccgtcac ggcgacggag aggaagacgc
     4021 ggtcggcgag ctgtgggccg gcggctgcgg agcaggcgcc gagggtcgcg acgagggtga
     4081 gcaggcagag gccgatggca agacgggttc ggtaggtcat cacggccgcg agacgcgggc
     4141 tcggcgtatc gagttccgcc gtgccccggc gatgcactcc atcggcgggc acgatagcct
     4201 cgacgcatgg atgacagcgg ctcgcccctg atctcggtcg atcgcctcgc cgactggctg
     4261 gcgagccccg agccgccggt cgtcgtcgac ttgcgctggt atctcggcaa gcccggcgcc
     4321 ggacgcgccg cgtacgagg
//
LOCUS       DATBSW010000096        12932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_465783, whole genome shotgun sequence.
ACCESSION   DATBSW010000096 DATBSW010000000
VERSION     DATBSW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12932
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465783"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..899
                     /locus_tag="VIF63_02650"
     CDS             138..899
                     /locus_tag="VIF63_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238310.1"
                     /translation="MSRPAPARRAVAALALQEVRLTARRGENVLVTLIIPVAVLLFFA
                     GTALVPGLAGRPIDTILPGAIALAIIATGLVNLGIATAYERSYGVLKRLGGAPLPRWG
                     LIAAKVTAVLALELVQVVLLVVVGSAVFVWAPGPGWSPLLLLGAITLGTVTFTGLGLL
                     LAGTLRAEATLALANGLFLAFLMLGGIVLPVDQLPAFLQPVAAVLPATALADLLRIAL
                     GAPAAAATSADAPALILGAWAVAATGLATAKFRWD"
     gene            complement(904..1326)
                     /locus_tag="VIF63_02655"
     CDS             complement(904..1326)
                     /locus_tag="VIF63_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238311.1"
                     /translation="MADPTGTAPKPPQIPDTAPQALKDFIDCRADSYAFADGPLEFLE
                     MQPKPKVAFTPGSTPDTATLAITVMGFTLKLPAAIRAGQLSIDTSGQWLMPGKVASDV
                     KRFTDDLNAWFASNGKQLSSPAFGPDGMTLTKVPLARA"
     gene            complement(1397..1471)
                     /locus_tag="VIF63_02660"
     tRNA            complement(1397..1471)
                     /locus_tag="VIF63_02660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1437..1439),aa:Met,seq:cat)
     gene            1668..2210
                     /locus_tag="VIF63_02665"
     CDS             1668..2210
                     /locus_tag="VIF63_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238312.1"
                     /translation="MHIPTWRVALTGGAITILAIAGIGFAAAANGPATPPAQPAAAEA
                     TAATEATAAPKASGRPDRPFAGERPAKGDGLRAARLLRLGRHLVHAEATITGRDGELI
                     VLQLDHGTVASIGGGSLTIAEAGGGSETVSTDDKTIVYVGRKDGELSDVTAGAEVFVQ
                     SRIEGSNVLAKRILVIPANT"
     gene            2272..3519
                     /locus_tag="VIF63_02670"
     CDS             2272..3519
                     /locus_tag="VIF63_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8238313.1"
                     /translation="MRPYSADSPWDFEPGLTFLTHGTFGACPRPIVERRMELIRELEA
                     NPIRFYTRDYEERFDAARREVAAFLGADPAGTVIVPNATTGVATVLESLRLRPGDELL
                     TNDHEYNATLNALETVAARAKARVVRVSIPLPIRHPEEVVEAILRGVTPHTRLALISH
                     VTSPSGLVFPIEAIVRELARLGVETLVDAAHAPGMVPVDVDALGAAYWTGNGHKWLCG
                     PKSVGVLVVREDRRTGVLPLVTGHGRNDLRLDRPALWREFDWPGTADTTAFFALPEAL
                     RLIGGLQPGGWPAHMAANHALAIAGRQLLNDRLGLEPLAPESMIGAMASIRMPTVLDE
                     DGANAIAHSLAAEERIEVPIAVFPVRAARAPGAPPSENLLRISAQRYNELADYELLAD
                     ALVRRGLGRAHAVARGASAIVAG"
     gene            3516..4799
                     /locus_tag="VIF63_02675"
     CDS             3516..4799
                     /locus_tag="VIF63_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8238314.1"
                     /translation="MTETVERATDATQAAAEEQLDQLKFLHRVARMATTASTWDELLE
                     TVVDETRDALRASVSSLYLLDRDAENLTLAATNGLDRYQIGKARVPFGDGVTGRVAAT
                     REPLVIPDVSHDDRFLWVRGIDQKRFIASMLSVPLVWNDRTVGVLNVQTEQRRDFTPS
                     DVAHLRAIADLLGGIVEKGRMQREAEARATELKAIDEARAELIAMVTHELRTPLAVVR
                     AYTELLAEEPPLEGRESRDTERRDRRAGWYRSTMDQVARLDRLVDSILASVRVDAEPG
                     ADLQPTDVGQVVGEATAELAPLLARHRLERREGIRLFALTDPSRLRQILEHLVENAVK
                     YAPPDTTIRLDWRMLDGSVTIGVEDEGPGIPEEWRERIFEPYARRETHTARGSGIGLF
                     AARRLAESVGARLWCEPAKGHGARFVIALPAAAAM"
     gene            4815..5525
                     /locus_tag="VIF63_02680"
     CDS             4815..5525
                     /locus_tag="VIF63_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8238315.1"
                     /translation="MARPLRILVVDDDPAMVGAITALVGTEGHQVITAYDGLTAVRRF
                     REERPDIVLLDLAMPGPDGFTVAGRIRAEGDAPIVVVSGESSEDAKVRALGIGADDYL
                     VKPFGRAELLARIAAVMRRAGRMSSGGSSAGDGDGSATTLRAGNLELDLGRHEAHVAD
                     QSLDLTPIEFRLAEALVRANGDLVPHLRLVRAGWPAEADPDLLWLKPHIARLRGKVET
                     AGGPPIIAVRGVGYRIGL"
     gene            complement(5788..7791)
                     /locus_tag="VIF63_02685"
     CDS             complement(5788..7791)
                     /locus_tag="VIF63_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238316.1"
                     /translation="MTQQRGPGGPGAGETRPSGPPGPPMRRPGGGPGMFGMSMPPAKP
                     KDFKGSFRRLLDRLGPERPLITIVIVLAVISVAFAVIGPKILGNATNDIFAGVLSQQI
                     PAGVTQEQMIEGLRARGENQLADMLASMKLTPGQGVDFNDLAGVLALLIGVYLISSLF
                     GWIQNWIMAGVTQRTILRLREEVDQKLGRLPLKYFDNHPRGDVLSRVTNDIDNIGQTL
                     QQSLTQLITSILTIVGVLILMFVISPILAVISILAVPASIVVTVLIATRSQKQFAAQW
                     ASTGTLNGHVEEMHTGHAIVKVFGRQEEAIDRFDQENAKLYESSYRAQFISGIIQPAL
                     NFVSNLNYVAIAVIGGVRVATGQMSLGDVIAFIQYSRQFTFPIVQAASIANVLQSAVA
                     SAERVFELLDEPEEIADPVVPVRLRSTRGAVAFEDVSFRYLPDVPLIDNLDLVADAGH
                     TIAIVGPTGAGKTTLVNLLMRFYELDGGRISIDDVDTREMTRDDLRRTFGMVLQDTWL
                     FHGTIRENIAYGRDGATDAEIASAADAAHVDHFVRTLPDGFDTVIDDDATNLSAGEKQ
                     LLTIARAFLADPAILILDEATSSVDTRTEVLIQRAMARLMKDRTAFVIAHRLSTIRDA
                     DTILVMDKGHIVEQGNHAELIAARGFYADLYESQFAEALAEAV"
     gene            complement(7788..9557)
                     /locus_tag="VIF63_02690"
     CDS             complement(7788..9557)
                     /locus_tag="VIF63_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238317.1"
                     /translation="MIRLLRRYLQPYKGRIVLVLALLLVQAIGNLYLPTLNGDIINEG
                     VAKGDTDYILNVGGLMLVVTLALAITAVLGVYWGAQVAMGFGRDVRSAIFRTVQTFSQ
                     VEVNRFGAPSLITRNTNDVQQVQTVVFLALTLIISAPMLIVGGIIMALRQDVPLSGLL
                     VVVLPLMVAFIGLVMSRAIPLFQATQVKLDRINQVMRETLAGVRVIRAFVRTRHEEAR
                     FQEASVDLFETSLKVNRLFAITLPVMTLILNLSGVAVMWFGAMRVDSGEMPIGNLTAF
                     LQYLMQILFTVLMAVFIFVFVPRAAVSAGRIQEVLEAEPSVRDPKAPRKLLVSSGNGT
                     GPVGGPVGGRIEFRNVEFGYPGAQDAVIKSASFVAEPGETLAIVGSTGSGKSTLINLI
                     PRFYDATSGAVLVDGIDVRELDRQDLWARLGVIPQKAFLFSGTVASNLRFGDASATDE
                     QLWHALEVAQAADFVREMEGGLEAPITQGGTNVSGGQRQRLAIARALVKRPAILIFDD
                     AFSALDVGTDSRLRAALDRDMGDATRIVVAQRVGTIMKADRILVMDEGRIVGTGTHAE
                     LLESNETYREIVYSQLSAEEAAA"
     gene            complement(9554..10054)
                     /locus_tag="VIF63_02695"
     CDS             complement(9554..10054)
                     /locus_tag="VIF63_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8238318.1"
                     /translation="MTLNIDPTLKTARATKARRGPLVAEVVDELTAWNPREMIAAFQR
                     WHQGSISLIQLNVLAFLEASGPLSMSRLADALDISVASITGVVDRMEARGLVERRRDS
                     DDRRVILVHPAEGARKLFADIDNRRRAGLSKLLTALSEEELRGLLAGHRALRAARAEY
                     IKQAPR"
     gene            10122..12218
                     /locus_tag="VIF63_02700"
     CDS             10122..12218
                     /locus_tag="VIF63_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8238319.1"
                     /translation="MAARTDPASVIRAQVVLDEHDMSADGRFAVVVRRFVVADRYRSH
                     LWLVPLAGRGQPIRLTSGPVRDTGPRLAPDGSAVAFRRSAAPTPGRRHRGTSTDPQDE
                     IARLRVLPLSSDGAAAGAPWAVRGPKARSIGEVAWSPDASRLAVTIEVDPPRFLVGPE
                     PKGDDAPTARRITRIDWRWDEQGAIDRWSHLHVVDARRGAQPRQVTSGDWGVSKVAWS
                     PDNRTVAFASDQRTDADLRPKESIWAVDVDADDAAPREVLALGSTVDKPAFSPDGRWI
                     SAIGYVAEDALDDTSPELVVGPGDGSSPPWPLAPQLDRPLGAWNDTDLHGWMANSRTT
                     PVWVDDSTIVATVTVRGRAAIWRYSIDPTTGHPASPPVSLGNGSIDAYSLAGTANANV
                     PRDRRVTCLSCIGTGPMDLITVSLDPARTPARKTTIGGRWSRSFDWPEMREIDIPGPG
                     GPIHTWIASPAGAGDAALPTIIDVHGGPLGGWAPSPSIEVILLCARGYRVILPNIRGS
                     TGYGRDWIRPHLGNWGGPDAADVHAALDHVIAVGLADPDQLGVLGLSYGGFMVNWLVG
                     TTDRFKAGVSIAGVTNQVSAWANGDAAVEFNRMSLLGTPLDPDGVARLWRQSPLSNVA
                     NVRTPLLLLQGEADLRCGPEDNVQLFMALRVLERTVEYVLYPEESHVYFASGRPDRRI
                     DHMTRMLDWFDRYLRA"
     gene            complement(12438..>12932)
                     /locus_tag="VIF63_02705"
     CDS             complement(12438..>12932)
                     /locus_tag="VIF63_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238320.1"
                     /translation="PGENRLWLHRGPGAPSRLELPLAPAGAEAVKPPVFGTAAPVLAE
                     VGSETSEPTRWEVVDDVTAGEVAIHTHEASVSVLPDGVSTLFVGETLTMAASEREPGT
                     GRFENDCEYRLESEGHRIVITADGGIQTSATAFDMTAHVKVELDGAPFFERTWREEIA
                     RDLL"
BASE COUNT         1925 a         4492 c         4560 g         1955 t
ORIGIN      
        1 tccgccgatg tcgtcgccga gatcgcgggc tgggcgctgc gcgaaggggt gctcatcgtc
       61 gagctgcggg ccggcgccgg ctccctcgag gagcgctacc tcgagctgac cggcgatcgc
      121 gccgtcgagg acgtcgaatg agcaggcccg caccggctcg gcgtgcggtc gccgcgctcg
      181 cgctccagga ggtgcggctg acggcgcgcc gcggcgagaa cgtgctcgtg accctgatca
      241 tcccggtcgc ggtcctgctg ttctttgccg ggactgcgct cgtgcccggc ctcgcgggcc
      301 gcccgatcga caccatcctg ccgggtgcga tcgccctggc gatcatcgcg accgggctcg
      361 tcaacctcgg catcgcgacg gcctacgagc gctcgtatgg ggtcctgaag cggctcggcg
      421 gcgcgccgct cccgcggtgg ggactcattg cggcgaaggt gaccgcggtt ctcgcactgg
      481 agctcgtgca ggtcgtcctg ctcgtggtcg tcgggtccgc cgtcttcgtg tgggcaccgg
      541 gtcccggctg gtcgccgctg ctgctcctcg gcgcgatcac cctcggcacg gtgacattca
      601 ctggcctggg cctgctgctc gcgggcacgc tccgcgccga ggcgacgctc gccctcgcga
      661 acggcctgtt cctcgcgttc ctgatgctcg gcgggattgt cctgccggtc gatcagctgc
      721 cggcattcct ccagccggtt gccgccgtcc tgcccgcgac cgccctcgca gacctgctcc
      781 ggatcgcgct cggtgccccc gccgcggcag caacctcggc ggacgcgccg gcgctcatcc
      841 tcggcgcctg ggccgtcgcc gcgaccgggc tcgcgacggc gaagtttcgc tgggactgag
      901 ccctcacgcc ctcgcgagcg ggaccttggt cagggtcatg ccgtccggac cgaatgccgg
      961 cgacgacaac tgcttgccat tcgaggcgaa ccaggcgttg aggtcgtcgg tgaagcgctt
     1021 gacgtcactg gcgaccttcc cgggcatgag ccattggccc gacgtgtcga tggacagctg
     1081 tccagccctg atcgcggcgg gcagcttgag ggtgaagccc atgaccgtga tcgcgagcgt
     1141 cgcggtgtcc ggggtcgaac ccggcgtgaa cgcgaccttc ggcttcggct gcatctcgag
     1201 gaactcgagc ggaccgtcgg cgaaggcgta gctgtcggcc cggcagtcga tgaagtcctt
     1261 cagcgcctgc ggcgccgtgt cggggatctg tggcggcttc ggtgcggtgc ccgtcgggtc
     1321 agccatcgat cgctccttca ccgaaaaaga agaaccccgg gtggtcggga cccggggttc
     1381 tgagatgact tcgaattggt tgcgggggaa ggattcgaac ctccgacctt cgggttatga
     1441 gcccgacgag ctgccgctgc tccaccccgc gagggctatc tactctagcc gaaaacgagc
     1501 ctttcgtcaa agcccagcta cccgcaaacc tcctcgctga gcatgaaacg gccgcttacg
     1561 cggcgctgac atggcgcggt catcatgctc acccgtaccg ttgaaggtcc gcgaacggcc
     1621 ggctctcccg gccaccaact atcgtcgaac gacacggagc acacccgatg cacatcccga
     1681 cctggcgcgt cgccctcacc ggcggcgcca tcacgatcct cgccatcgcc ggcatcggct
     1741 tcgccgcggc ggcaaacgga cccgcaactc ctcccgcgca gcccgcggct gcggaagcaa
     1801 cggcggccac ggaggcgacg gcggccccga aggcgagcgg ccggccggat cggccgttcg
     1861 ccggcgagcg tcccgccaag ggcgatggcc tgcgggctgc gcgcctcctg cggctcggcc
     1921 gccacctcgt ccacgccgag gccaccatca cgggccgcga cggcgagctg atcgtcctcc
     1981 agctggacca cggcactgtc gcctcgatcg gtggcggcag cctcacgatc gcggaggccg
     2041 gcgggggcag cgagaccgtc tcgaccgacg acaagacgat cgtctacgtc gggcgcaagg
     2101 acggcgagct ctccgatgtc acggcgggcg ccgaggtgtt cgtccagagc cggatcgagg
     2161 gctcgaacgt cctggcgaag cgaatcctgg tgatccccgc aaacacctaa gcgaaaccgc
     2221 gaccgacgac gggcgggtgc tggggacccg cccgtcgcta tgctcggggc catgcgcccg
     2281 tactccgctg acagcccgtg ggacttcgag ccggggctca cgttcctgac ccacggcacg
     2341 ttcggcgcgt gtccgcgccc gatcgtcgag cggcggatgg agctgatccg tgagctcgag
     2401 gcgaacccga tccggttcta cacccgggac tacgaggagc gcttcgatgc ggcccggcgc
     2461 gaggtcgcgg cattcctcgg ggcagacccg gccggcacgg tcatcgtccc gaacgcgacg
     2521 accggggtgg ccacggtcct cgagtcgttg cgccttcgac ccggcgacga gctgctcacg
     2581 aatgaccacg agtacaacgc aaccctgaat gcgctcgaaa cggtcgccgc cagggcgaag
     2641 gcgcgggtcg tccgggtgtc gatcccgctg ccgatccgcc atcccgagga ggtcgtcgag
     2701 gcgatcctcc gcggggtcac gccgcatacc cgcctggcgc tgatcagcca tgtgacaagc
     2761 ccgagcggcc tcgtcttccc gatcgaggcg atcgtccgcg agctggcccg cctcggcgtc
     2821 gagacgctgg tcgatgcggc gcatgcgccg ggcatggtcc ctgtcgacgt cgacgcgctc
     2881 ggtgccgcgt actggaccgg caacgggcac aagtggctgt gcgggccgaa gagcgtcggg
     2941 gtcctcgtcg tgcgcgagga tcgacgaacc ggcgtcctgc ccctcgtgac cggccatggc
     3001 cgaaacgacc tgcggctcga ccgacccgcc ctgtggcgcg agttcgactg gccgggtacc
     3061 gccgacacga ccgcgttctt cgccctgccc gaggcgctcc ggctcatcgg cggcctccag
     3121 ccgggcggct ggccggcgca catggcggcg aaccacgcgc tggcgattgc gggccggcag
     3181 ctgctcaacg accggctcgg gctcgagccg ctcgccccgg aatcgatgat cggcgcgatg
     3241 gcgtcgatcc ggatgcccac ggtcctggac gaggatggcg ccaacgcgat cgcgcactcg
     3301 ctcgccgccg aggagcggat cgaggtgccg atcgcggtct tcccggttcg tgcggcgcgg
     3361 gccccgggtg cgccgccgag cgagaacctg ctgcggatct ccgcacagcg ctacaacgaa
     3421 ctcgccgact acgagctcct tgccgacgcg ctggtgcggc gagggctcgg gcgagcgcac
     3481 gcggtggcgc gaggcgcctc ggctatcgtg gcggggtgac cgagaccgtc gaacgcgcaa
     3541 ccgacgcaac gcaggctgcc gccgaggagc agctcgacca gctcaagttc ctgcaccgcg
     3601 tcgcgcgcat ggccacgacc gcgagcacgt gggacgagct cctcgagacg gtcgtcgacg
     3661 agacacgcga tgcgctccgg gcgagcgtgt cgtcgctgta cctgctcgat cgtgacgccg
     3721 agaacctgac cctcgcagcg acgaacggcc tcgaccgcta ccagatcggc aaggcgcggg
     3781 tgccgttcgg cgatggcgta acgggccgcg tcgccgcgac gcgcgagccg ctcgtgatcc
     3841 cggacgtcag ccacgacgac cgcttcctgt gggtccgcgg catcgaccag aagcggttca
     3901 tcgcctcgat gctctccgtg ccgctcgtct ggaacgatcg gacggttggc gtgctcaacg
     3961 tccagacgga gcagcgccgc gacttcacgc catcggatgt cgcgcacctg cgtgcgatcg
     4021 ccgacctgct cggcggcatc gtcgagaagg gccgcatgca gcgcgaagcc gaggcccggg
     4081 cgaccgagct gaaggcgatc gacgaagccc gcgccgagct catcgcgatg gtcacgcacg
     4141 agctgcgcac gccgctcgcg gtcgtccgcg cgtacacgga gctgctcgcc gaggagccac
     4201 cgctcgaggg ccgcgaatcg agggacacgg aacgtcgcga ccgtcgggcc ggctggtacc
     4261 gctcgacgat ggaccaggtc gcgcgcctcg atcgcctggt cgactcgatt ctcgcctccg
     4321 tgcgtgtcga cgcggagccc ggcgccgacc tccagccgac cgacgtcggg caggtcgtgg
     4381 gcgaggcgac cgcggagctc gcgcccctgc tcgcccgcca tcggctcgag cgccgcgagg
     4441 gcatccgcct gttcgccctg accgacccga gccggctgcg ccagatcctc gagcatctcg
     4501 tcgagaacgc ggtcaagtac gccccgccgg acaccacgat ccggctcgac tggcggatgc
     4561 tcgacggcag cgtgacgatc ggcgtcgagg acgaggggcc gggcatcccc gaggaatggc
     4621 gcgagcggat cttcgagccg tacgcccgac gcgagacgca cacggcgcgc gggtcgggga
     4681 tcgggctgtt cgcagcgcgg cggctcgcgg aatccgttgg cgcccgactc tggtgcgagc
     4741 ccgcgaaggg ccacggcgcg agattcgtca tcgccctgcc cgccgcggcg gcgatgtagg
     4801 ccgcgatcac aacgatggca cgcccgctcc gcatcctcgt cgtcgacgac gacccggcca
     4861 tggtcggggc gatcaccgcg ctcgtcggca ccgagggcca ccaggtcatc accgcctacg
     4921 acggactgac cgcggttcgc cgcttccgcg aggagcgccc ggacatcgtg ctgctggatt
     4981 tggcgatgcc cggcccggat gggttcacgg tcgccggccg gatccgcgcc gagggcgacg
     5041 ccccgatcgt ggtcgtgtcg ggcgagagct ccgaggacgc caaagtgcgc gcactcggca
     5101 tcggcgcgga cgattacctc gtcaagccgt tcgggcgcgc ggagctgctg gcgcgaatcg
     5161 ccgcggtcat gcgtcgtgcg ggccggatgt cgagcggcgg ctcgtcggcg ggtgacggcg
     5221 atggcagcgc gacgacattg cgcgccggca acctcgagct cgacctgggc cggcacgaag
     5281 cgcacgtcgc cgatcaatcg ctggacctca cgccgatcga attccgcctc gccgaggccc
     5341 tggtccgcgc aaacggcgac ctcgtccccc acctccgcct ggtccgcgcc ggttggcccg
     5401 ccgaggccga cccggacctg ctctggctga agccccacat cgcccgcctc cgcggcaagg
     5461 tcgagaccgc cggcggcccc ccgatcatcg ccgtgcgcgg cgtcggctac cgcatcgggc
     5521 tctgagggca aaggctcacg gtcgatcgtt catcaaatgt tcgatgcggc ggctgaagac
     5581 gctgtcgagg cgtggaaaga cgccgtcgcc cgtcgggtcg aacatctgat gttcggcgcg
     5641 accgccgagc ggccgattcg ccgtggttcg aggcatccca gccgtcggat cgttcatcca
     5701 atgaaggtct accggggcgg ggcgggccgg ggcggctcgg ctcgactcgg accgggcgcg
     5761 gcgcattcga taggaggcgc cagatcgtca gaccgcctcg gcgagggcct cagcgaactg
     5821 cgattcgtag aggtcggcgt agaagccgcg ggcggcgatg agctcggcgt ggttgccctg
     5881 ctcgacgatg tggcccttgt ccatcacgag gatcgtgtcc gcgtcgcgga tcgtggagag
     5941 gcgatgggcg atcacgaacg cggtgcggtc cttcatcagc cgcgccatcg cgcgctggat
     6001 gagcacctcg gtgcgggtgt cgaccgatga cgtcgcctcg tcgaggatca ggatcgccgg
     6061 gtcggcgagg aacgcccgcg cgatcgtgag cagctgcttc tcgcctgcgg acaggttcgt
     6121 ggcgtcgtcg tcgatgaccg tgtcgaagcc gtcgggcagc gtgcgcacga agtgatcgac
     6181 gtgcgccgcg tcggccgcgg aggcgatctc ggcgtcggtc gcgccgtcgc ggccgtaggc
     6241 gatgttctcg cggatcgtgc cgtggaagag ccacgtgtcc tggaggacca tgccgaacgt
     6301 ccggcgcagg tcgtcgcggg tcatctcgcg cgtgtcgacg tcgtcgatcg agatccgccc
     6361 accgtcgagc tcgtagaaac gcatcagcag gttgacgagc gtggtcttgc cggcgcccgt
     6421 cggaccgacg atcgcgatcg tgtggccggc gtcggccacg agatcgaggt tgtcgatgag
     6481 cggcacgtcc ggcaggtacc ggaatgagac gtcctcgaac gcgaccgcgc cgcgggtcga
     6541 gcgcaggcgc accggcacga cgggatccgc gatctcctcc ggctcgtcga ggagctcgaa
     6601 gacgcgctcg gcagacgcga cggccgactg gaggacgttc gcgatcgacg ccgcctggac
     6661 gatcgggaag gtgaactggc gcgagtactg gatgaacgcg atgacgtcgc cgaggctcat
     6721 ctggccggtc gcgacgcgca cgccgccgat gacggcgatc gcgacgtagt tgaggttcga
     6781 cacgaagttc agcgccggct ggatgatccc ggagatgaac tgcgcccggt agctcgactc
     6841 gtagagcttc gcgttctcct ggtcgaaccg gtcgatcgcc tcctcctgcc gcccgaagac
     6901 cttcacgatc gcgtggccgg tgtgcatctc ctcgacgtgg ccgttgagcg tgcccgtcga
     6961 tgcccactgc gcggcgaact gcttctggga gcgggtcgcg atgagcacgg tgacgacgat
     7021 cgaggcgggc acggcgagga tcgagatcac ggccaggatc gggctgatca cgaacatcag
     7081 gatcagcacg ccgacgatcg tcagaatcga ggtgatgagc tgggtgaggc tctgctggag
     7141 cgtctggccg atgttgtcga tgtcgttggt gacacggctc agcacgtcgc cccggggatg
     7201 attgtcgaag tacttgagcg gcagccggcc gagcttctgg tcgacctcct cgcggaggcg
     7261 aaggatcgtc cgctgcgtga cgccggccat gatccagttc tggatccagc cgaacagcga
     7321 gctgatcagg tacacgccga tcagcagcgc gagcacaccg gcaaggtcgt tgaaatcgac
     7381 accctggccg ggggtgagct tcatcgaggc gagcatgtcc gcgagctggt tctcgccgcg
     7441 ggcgcggagg ccctcgatca tctgctcctg cgtgacgccg gccgggatct gctggctcag
     7501 gacgccggcg aagatgtcgt tcgtcgcatt gccgaggatc ttcgggccga tgacggcgaa
     7561 ggcgacgctg atgaccgcga gcacgatcac gatcgtgatc agcggacgct cgggcccgag
     7621 ccggtcgagc agccgccgga acgatccctt gaagtccttg ggcttggccg ggggcatgct
     7681 catgccgaac atgccggggc cgccacccgg acgccgcatc ggtggaccgg gaggacccga
     7741 cggccgggtc tcgccggcgc ccgggccgcc ggggccgcgt tgctgcgtca tgccgcggcc
     7801 tcctcggcgg agagctgtga gtacacgatc tcgcggtacg tctcgttcga ctcgaggagc
     7861 tcggcatggg tgccagtgcc cacgatccgg ccctcgtcca tgaccaggat ccggtcggcc
     7921 ttcatgatcg tgccgactcg ctgcgcgacg acgatccggg ttgcgtcgcc catgtcccgg
     7981 tcgagcgcgg cacggaggcg cgaatccgtg cccacgtcga gcgcagagaa ggcgtcgtcg
     8041 aagatcagga ttgccggtcg ctttacgagc gcccgcgcga tcgcgaggcg ctgccgctgc
     8101 ccgcccgaga cgttcgtgcc gccctgggtg atcggggctt cgaggccgcc ctccatctcg
     8161 cgcacgaagt cggccgcctg ggcgacctcg agggcgtgcc acagctgctc gtcggtcgcg
     8221 ctcgcatcgc cgaagcgcag gttgcttgcg acggtcccgc tgaacaggaa tgccttctgg
     8281 ggaatgacgc cgaggcgcgc ccacaggtcc tgccggtcga gctcgcgaac gtcgatgccg
     8341 tcgacgagga ccgcacctga cgtcgcgtcg tagaaccgcg ggatcaggtt gatcagcgtg
     8401 gacttgccac tgccggtcga gccgacgatc gcgagcgtct caccgggctc ggccacgaat
     8461 gacgcgctct tgatcacggc gtcctgtgcg cccgggtacc cgaactccac gttccggaac
     8521 tcgatccggc cgccaaccgg gcccccaacg ggccccgtgc cattcccact gctgacaagg
     8581 agcttccggg gcgccttcgg gtcgcgcacg gacggctcgg cctcgaggac ctcctggatc
     8641 cggcccgcgg acaccgcggc gcggggcacg aacacgaaga tgaagacggc catcaggacc
     8701 gtgaacagga tctgcatcag gtactggagg aacgcggtca ggttgccgat gggcatctcg
     8761 ccgctgtcga cgcgcatcgc accgaaccac atgaccgcga cgcccgagag gttgaggatc
     8821 agggtcatca ccgggagggt gatcgcgaac aggcggttga ccttcaggga cgtttcgaag
     8881 aggtcgacgc tcgcttcctg gaagcgtgcc tcctcgtggc gcgtccgcac gaatgcccgg
     8941 atgacccgga cgccggcgag cgtctcgcgc atgacctggt tgatccggtc gagcttgacc
     9001 tgcgtcgcct ggaacagcgg gatcgcccgg gacatgacga gcccgatgaa cgcgaccatc
     9061 aacggcagca cgacgacgag caggcccgag agcggcacgt cctggcgcag cgccatgatg
     9121 atcccgccca cgatcagcat cggcgccgag atgatcagcg tcagggcgag gaagacgacc
     9181 gtctgcacct gctggacgtc gttcgtgttg cgggtgatca gcgacggcgc gccgaacctg
     9241 ttgacctcga cctggctgaa cgtctggacg gtccggaaga tcgcgctccg gacgtcgcgc
     9301 ccgaagccca tggcgacctg cgcgccccag tacacgccga ggacggccgt gatcgcgagc
     9361 gccagcgtga cgacgagcat cagcccgccg acgttgagga tgtagtccgt gtcgcccttg
     9421 gcgacgccct cgttgatgat gtcgccgttg agggtcggca ggtacaggtt gccgatcgcc
     9481 tggacgagga gcagcgcgag gacgagcacg atccggccct tgtagggctg gaggtagcga
     9541 cgcagcaggc ggatcatcgg ggcgcctgct tgatgtattc ggcgcgggcg gcgcgcagcg
     9601 cgcgatgccc ggcgaggagg ccgcggagct cctcttcgct cagcgcggtg agcagcttcg
     9661 acagccctgc ccggcggcgg ttgtcgatgt cggcgaagag cttccgagcg ccctctgcgg
     9721 gatggaccag gatgacccgg cgatcgtcgc tgtcgcggcg ccgttcgacg aggccgcggg
     9781 cttccatgcg gtcgaccacg cccgtgatgc tggcgacgga gatgtcgagg gcatcggcga
     9841 gccggctcat cgacagcggg ccggacgcct cgaggaatgc gagcacgttg agctggatca
     9901 ggctgatcga gccctggtgc caacgctgga acgcggcgat catctcgcgc gggttccagg
     9961 ccgtcagctc gtccacgacc tcggcgacga gcgggccccg gcgcgccttg gtggcgcggg
    10021 cggtcttcag cgtcgggtcg atattcaatg tcacgctgac aatctaccct gcatcgagca
    10081 atcgcgcaac ctcgggaccc cgcggcgggt acgctctgga cgtggccgca cgaacggatc
    10141 ccgccagcgt cattcgcgcg caggtcgtgc tcgacgagca cgacatgtcg gccgatgggc
    10201 ggtttgcggt cgtcgtccgg cggttcgtcg tcgccgaccg ataccgatcg cacctgtggc
    10261 tcgtcccact cgcaggacgc gggcagccga tccggctcac gagtggcccg gttcgcgaca
    10321 cgggtccccg cctcgcgccc gacggatcgg ccgtcgcgtt ccgtcgttcc gccgcaccga
    10381 cgcccggtcg ccggcacagg ggcacgagca ccgacccgca ggacgagatc gcgcggctcc
    10441 gcgtcctccc gctgtcgagc gacggcgccg ccgccggcgc gccatgggcg gtccgcggtc
    10501 cgaaggcgcg gtcgatcggc gaggtcgcct ggtcgcccga tgcctcgcgc ctcgccgtca
    10561 cgatcgaggt ggacccgcca cgattcctgg ttggcccgga gccgaagggc gacgacgcgc
    10621 ccaccgcccg gcgcatcacc cggatcgact ggcgatggga cgagcagggc gcgatcgatc
    10681 ggtggtcgca tctccatgtc gtcgatgccc gtcgcggcgc acagccgcgc caggtgacat
    10741 cgggcgattg gggcgtctcg aaggttgcct ggtcgcccga caaccggacc gtcgccttcg
    10801 cctcggacca gcgcaccgac gccgacctcc gcccgaagga atcgatctgg gcggtcgacg
    10861 tcgacgccga cgacgccgcg ccgcgcgagg tgctggccct cggctcgacg gtcgacaagc
    10921 ccgcgttctc gccggatggt cggtggatct ccgcaatcgg ctatgtggcc gaggacgcgc
    10981 tggacgacac gagccccgag ctggtcgtcg gacctggcga cggctcgtca ccgccgtggc
    11041 cgctcgcgcc ccagctcgac cgtcccctcg gcgcctggaa cgacacggac ctgcacggct
    11101 ggatggcgaa ctcgcgcacc accccggtct gggtcgacga ctcgaccatc gtggcaacgg
    11161 tcacggtccg cggccgcgcc gcgatctggc gctactcgat cgatccgacg acgggccatc
    11221 ccgcgagccc gcccgtgtcg ctcgggaacg ggtcgatcga tgcatacagc cttgccggca
    11281 ccgcaaacgc gaacgtcccc cgcgatcggc gggtcacctg cctgtcctgc atcggaaccg
    11341 gcccgatgga cctgatcact gtttcgctgg atccggcgcg gactccggcc cggaagacga
    11401 ccatcggtgg gcgctggtcg cgaagcttcg actggcccga gatgcgcgag atcgatattc
    11461 cgggtccggg cggcccgatc cacacctgga tcgcatcacc ggccggcgcg ggcgacgccg
    11521 cgctgcccac gatcatcgac gtgcatggcg gaccactcgg gggctgggca ccgtcgccat
    11581 cgatcgaggt catcctgctc tgtgcgcgcg gctaccgggt gatcctgccg aacatccggg
    11641 gatccaccgg ctacgggcgc gactggatcc ggccgcacct cggcaactgg ggtgggccgg
    11701 atgcggcgga cgtccacgcc gccctcgacc atgtgatcgc cgtgggcctc gccgaccccg
    11761 atcagctcgg ggtgctcggg ctgtcgtacg gcgggttcat ggtcaactgg ctcgtcggga
    11821 cgacggatcg cttcaaggcc ggcgtgagca tcgccggcgt cacgaaccag gtgtcggcct
    11881 gggccaacgg cgacgcggcg gtcgagttca atcggatgtc gctgctgggc acgccgctgg
    11941 atccggacgg ggtcgcgagg ctctggcggc agagcccgct ctcgaacgtg gccaacgttc
    12001 ggacgcccct gctcctgctg cagggcgagg cggacctgcg ctgcgggccg gaggacaacg
    12061 tccagctctt catggcgctg cgggtgctgg aacggacggt ggagtacgtg ctctacccgg
    12121 aggaatcgca cgtgtacttc gcatcaggac gacccgaccg caggatcgat cacatgaccc
    12181 gcatgctcga ctggttcgac cgctacctgc gcgcataggg tcgcggctgc gagttcagtt
    12241 tgtgtcaggt gttcctcacc cgggcgctcg tcagggcaag gacgccaaga gcatcgctcg
    12301 cacgctcagc cagcggcgct ggctcggaca gatgctgccc gacgggcgca tttggccgga
    12361 tcctcgtgac gagcgcccgc atcagtaaca gatgacacat ccggggcctc acggggtcgg
    12421 tttcggcgag tcgaccccta gagcaggtcc cgggcgatct cctcgcgcca cgttcgctcg
    12481 aagaagggcg cgccgtcgag ttcgaccttc acgtgggccg tcatgtcgaa tgccgtcgcg
    12541 gaggtctgga tcccgccgtc ggcggtgatg acgatccggt ggccctcact ctccaggcga
    12601 tactcgcagt cgttctcgaa gcgaccggtg ccgggctcac gctcggaggc ggccatcgtg
    12661 agcgtctcgc cgacgaacag ggtgctgacg ccgtccggga gcacgctgac cgatgcctcg
    12721 tgcgtgtgga tcgcgacttc gccggccgtc acatcgtcga cgacctccca gcgcgtcggc
    12781 tcgctcgtct cgctgccgac ctccgcgagc acgggcgccg ctgtgccgaa caccggcggc
    12841 ttgacggcct cggcaccggc tggcgcgagt ggcagctcca gccgggaggg ggcaccaggt
    12901 cctcgatgca gccagagccg gttctcgccc gg
//
LOCUS       DATBSW010000097         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_465886, whole genome shotgun sequence.
ACCESSION   DATBSW010000097 DATBSW010000000
VERSION     DATBSW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465886"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..799)
                     /gene="pth"
                     /locus_tag="VIF63_02710"
     CDS             complement(56..799)
                     /gene="pth"
                     /locus_tag="VIF63_02710"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY8238321.1"
                     /translation="MFGSEVRQINKIVVGLGNPGDQYRQTRHNVGWMVLDRLADRAGW
                     DGKGRSKDASAVVGGRFRGLDLLLVKPQTYMNDSGIAVRKVLARERAPLTEMLVVVDD
                     FALPFGKLRFREGGGPGGHNGLRSIIGELESEAFSRLRVGIGAPNDRFIDHVLTKFEP
                     DERQRLDELLDAAADAVEGWARDGTNKAATRFNAFELRPADTELAAPAGEIGGPPGPD
                     GIRRTTTGWRKLRTKITGSDEPDGDGDRP"
     gene            complement(861..1385)
                     /gene="ybeY"
                     /locus_tag="VIF63_02715"
     CDS             complement(861..1385)
                     /gene="ybeY"
                     /locus_tag="VIF63_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8238322.1"
                     /translation="MTIRQLPERTRVVYRPPWRIDVVQRAGIWSPISASAIAAAATAA
                     LEAAGAPAPGSVTIVLTDDPELAELNLEHMGLEGATDVLSFPMLQPTEFPSHAEAGGR
                     RTHIGDIAISVERAIEQAAEGRGGQTGDVAWSAEDELRLLVTHGTLHLCGHDHAEPGE
                     ELAMRTLERQLLEA"
     gene            complement(1411..3600)
                     /locus_tag="VIF63_02720"
     CDS             complement(1411..3600)
                     /locus_tag="VIF63_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HEY8238323.1"
                     /translation="MSGNGSTPAGGGADERRWPNRRELIRLFIAAGSLILIMTVTLGL
                     DLSPGLDVKVGELAPSDIRAPRALTFTNQLLTDEARAQARLGVPPQYDYTSERAITIA
                     AEQLNDFTRRVAALDTAFAPETSAEDRQALLDAVLPGLSDDARTVLQTMEPERWASIK
                     TEAARVLDVTERSELRDTEVAQARQRLSAQMAGDLSDSERVLAAELIGPLLVPNSSFS
                     SLRFQQEQDRRAAAVQPVIENIVQGQVIVREGAKLTEVDLARIQALALDVQRLDVAAL
                     GGWLLISSLLVSMLIAWLRLFRPEYWHRNNVVLLIWLLVALATFALQITEGRAALPFV
                     LPVAAIGLLVTVLLDAETAIIATAAVAIVAGAVNGPSLELGAYAMLGGVAGILAIRRG
                     DRLQTFIQAGAAIFVVQAVVVTAFSLLGERDLTGVVTLIGAAGVSAGIAAVVAVGSFA
                     VLGSVFGLLTVFQLLELANPSQPLLRRLLVETPGTYHHSLMVGNLAERAAETIGADPL
                     ITRVAAYYHDVGKLANPLAFIENQSGGENVHDELEPGESAQILKQHVADGIDVAYRSR
                     LPKSLIAFIPQHHGTAVMSYFLAKAREEAAAPFGGLATEEGKKAADAVDARRYRHAGP
                     KPQSREAALLMLADGVEASVRSLSSRDEPAIRSMVARIISERMDDGQFDECDLTLRDI
                     NRAQEAFVSQLLGMYHQRVAYPQNKVVEIESRRAAGGSGGGGGGTSA"
     gene            complement(3620..4081)
                     /locus_tag="VIF63_02725"
     CDS             complement(3620..4081)
                     /locus_tag="VIF63_02725"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8238324.1"
                     /translation="MSLRERILSDITAAMRSGAALRRDTLRMAESAIYNAEKRDRRTY
                     SDDDVAGILAREVKTRRESVEAFRKGGREDLAEKEDLEIAILSEFLPDQLSGEEIAAL
                     IAEAVAATGATTPKDMGKVMGWLAPRTRGRADGRVVSQAVTAALSGAGAGS"
BASE COUNT          580 a         1509 c         1521 g          659 t
ORIGIN      
        1 cgctctgcga gacgccgctc ccagccacgc ttgccgcgca tcggcgggct gctgctcacg
       61 ggcggtcacc gtcaccgtcg ggctcgtcgc tgccggtgat cttcgtgcgg agcttccgcc
      121 agcccgtcgt cgtccgccgg atgccatcag gtccgggcgg cccgccgatc tcgccggccg
      181 gcgcggccag ctccgtgtcc gcgggccgga gctcgaacgc gttgaaccgc gtcgcggcct
      241 tgttcgtgcc gtcacgcgcc cagccctcga ccgcgtcggc ggcagcgtcg agcagctcgt
      301 cgagccgctg gcgctcgtcg ggctcgaact tcgtcaggac gtggtcgatg aagcggtcgt
      361 tcggcgcccc gatgccgacc cgcagccggc tgaacgcctc cgactcgagc tcgccgatga
      421 tcgagcgcag gccgttgtgc ccaccgggcc cgccgccctc gcggaagcgc agcttgccga
      481 acgggagcgc gaagtcgtcg accacgacga gcatctcggt cagcggcgcg cgctcacgtg
      541 cgagcacctt gcggaccgcg atgcccgagt cgttcatgta cgtctggggc ttcacgagca
      601 gcaggtcgag gccgcggaat cgcccgccga cgaccgccga cgcgtccttg ctccgaccct
      661 tgccgtccca gccggcccgg tccgccaggc gatcgagcac catccagccg acgttgtgcc
      721 gggtctgacg gtactggtcg ccggggttcc cgagcccgac cacgatcttg ttgatctgtc
      781 gcacctcgct gccgaacacg ccgaactgcc ccagaacgtt gttccggggc tcgcgccaat
      841 ggtacccggc gtgcggtcgg ctaggcctcg agcagctgtc gttccagcgt gcgcatcgcc
      901 aactcctcgc cgggctccgc gtggtcgtgg ccgcagaggt ggagcgtgcc gtgcgtcacc
      961 aggagtcgga gctcgtcctc ggccgaccag gcgacgtcgc cggtctgccc gccacgcccc
     1021 tcggccgcct gctcgatcgc acgctccacc gagatcgcga tgtcgccgat gtgcgtgcgg
     1081 cgcccgccgg cctcagcgtg gctcgggaac tcggtgggct gcagcatcgg gaacgacagc
     1141 acgtccgtcg ctccctcgag gcccatgtgc tcgaggttga gctcggccag ctcggggtcg
     1201 tcggtcagca cgatcgtgac ggagcccggc gccggcgcgc cggcagcttc gagggcggcg
     1261 gtcgcggcgg cggcgatcgc tgacgccgag atgggcgacc agatgcccgc tcgctgcacg
     1321 acgtcgatgc gccacggcgg ccgatagacc acccgggttc gttccggcag ctgtcgaatg
     1381 gtcaccggac gcgcacgacc gcccgtcggc tcacgcggat gtgccgcctc cgccgccgcc
     1441 gctcccgccg gccgcgcgcc gcgactcgat ctcgacgacc ttgttctgcg gatacgcgac
     1501 gcgctggtgg tacatcccga gcagctggct gacgaaggcc tcctgtgccc ggttgatgtc
     1561 gcggagggtg aggtcgcact cgtcgaactg gccgtcgtcc atgcgctcgc tgatgatgcg
     1621 ggcgaccatc gatcggatcg ccggctcgtc acgcgacgac agcgatcgca ccgatgcctc
     1681 cacgccgtcg gcgagcatga gcagggccgc ctctcgtgat tgcggcttcg ggccggcgtg
     1741 gcgatagcgg cgcgcgtcga cggcgtcggc cgccttcttg ccttcttcgg tggcgagccc
     1801 gccaaatggc gccgccgcct cctcgcgggc cttggcgagg aagtagctca tgaccgcggt
     1861 gccgtggtgc tgcgggatga aggcgatgag cgacttgggc aggcgcgagc ggtaggcgac
     1921 atcgatgccg tccgcgacgt gttgcttcag gatctgcgcg gactcgccgg gctcgagctc
     1981 gtcgtgcacg ttctcgccac cggactggtt ctcgatgaag gcgagcgggt tcgcgagctt
     2041 gccgacgtcg tggtagtacg ccgcgacgcg ggtgatcagc gggtcggcgc cgatcgtctc
     2101 ggcggctcgc tccgccaggt tcccgaccat gagcgagtgg tggtacgtgc ccggcgtctc
     2161 cacgaggagc cggcgaagca gcggctgcga cgggttcgcg agctcgagga gctggaagac
     2221 cgtcagcagg ccgaagacgc tgccgaggac ggcgaagctg ccgaccgcga ccacggcggc
     2281 gatgcccgcc gacacgcccg cggcgccgat cagcgtcacg acgccggtca ggtcccgctc
     2341 tccgagcagc gagaacgcgg taacgacgac cgcctggacc acgaagatcg cggcgccggc
     2401 ctggatgaac gtctggagcc ggtcgccgcg ccggatcgcg aggatgccgg cgacgccgcc
     2461 cagcatcgcg tacgccccga gctcgaggga cggcccgttc acggcgccgg ccacgatcgc
     2521 cacggccgcc gtcgcgatga tcgcggtctc ggcgtccagg agcacggtga cgagcaggcc
     2581 gatcgctgct accggcaaga cgaacggcag cgcggcgcgg ccctcggtga tctggagggc
     2641 aaaggtggca agggcgacca gcagccagat cagcagcacg acattgttcc ggtgccagta
     2701 ctcgggccgg aacaggcgca gccaggcgat gagcatcgag acgagcagcg acgagatgag
     2761 gagccagccg ccgagcgcgg cgacatcgag ccgctgcacg tcgagcgcga gggcctggat
     2821 ccgcgcaagg tcgacctcgg tcagcttcgc gccttcgcgg acgatgacct ggccctggac
     2881 gatgttctcg atcaccggct ggacggccgc cgcgcgtcga tcctgctcct gctggaatcg
     2941 gagcgagctg aacgagctgt tcgggacgag cagcggcccg atcagctccg cggcaaggac
     3001 gcgttcgctg tcggagaggt caccggccat ctgggcggag aggcgctggc gcgcctgggc
     3061 gacctcggtg tcacgaagct cgcttcgctc ggtgacatcg aggacgcgcg cggcctccgt
     3121 cttgatcgat gcccagcgct cgggctccat cgtctggagg accgtgcggg cgtcgtcgct
     3181 gaggcccggc aggaccgcgt ccagcagcgc ctggcgatcc tccgccgacg tctccggagc
     3241 gaacgcggta tcgagcgcgg ccacgcgccg ggtgaagtcg ttgagctgct ccgccgcgat
     3301 cgtgatcgcg cgctcggagg tgtagtcgta ctggggcggc acaccgaggc gggcctgcgc
     3361 gcgcgcctca tcggtcagga gctggttcgt gaacgtgagg gcgcgcgggg cacggatgtc
     3421 gctcggggcg agctcgccga ccttgacgtc gaggcccggc gaaaggtcca ggccgagcgt
     3481 cacggtcatg atcaggatca acgagccggc cgcgataaag agccggatca gctcgcgccg
     3541 attcggccag cgacgctcgt ccgcgccgcc gcctgcgggc gtcgacccgt tcccgctcac
     3601 gtgccggcgc tctggatgct cacgagccgg cgcccgcgcc ggaaagcgcc gccgtcaccg
     3661 cctggctgac gactcggccg tcggctcggc ccctcgttcg gggcgcaagc cagcccatga
     3721 ccttgcccat gtccttcggg gtggtcgcgc ccgtggctgc gaccgcctcg gcgatgagcg
     3781 ccgcgatctc ttccccggag agttgatcgg gcaggaactc ggacaggatc gcgatctcga
     3841 ggtcttcctt ctcggcgagg tcctcgcggc cgcccttccg gaacgcctcg accgactcgc
     3901 gccgcgtctt cacctcgcgt gcgaggatgc ccgcgacatc gtcatcggaa tacgtgcggc
     3961 ggtcgcgttt ctcggcgttg tagatcgcgc tttcggccat gcgcagggtg tcgcggcgca
     4021 gcgccgcacc cgaccgcatc gcggccgtga tgtcggagag gattcgctct cgaagggaca
     4081 ccggtatcgg cacctcgggc ggagatatgc ccgggccgtc gattggaggg cggaacgtcg
     4141 atcgcgatgc tgcgcgtcgg acggtcccgg ccgtccggtc gatcagccct gtggcgttcg
     4201 cgcgttcttc ttgcgcttcg cttccttgcg cttccgcttc acgctcggct tctcgtagtg
     4261 ctcgcgtcg
//
LOCUS       DATBSW010000098         5363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_289692, whole genome shotgun sequence.
ACCESSION   DATBSW010000098 DATBSW010000000
VERSION     DATBSW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289692"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3850
                     /locus_tag="VIF63_02730"
     CDS             <1..3850
                     /locus_tag="VIF63_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8238325.1"
                     /translation="LVRARTRIAAVLAALTLLTTSLVIPASAAKPPAPGALATKALFY
                     AADGMRPDKVDAYAAQGVMPNMKALAAAGVKGVNGMQPGFPPNTGVGWYTMMTGTWPS
                     EHGSTNNTYHRTGEGNFNNRTSFSALQGAAFDQPTIQADTLAAAAERAGKKVAQIDWV
                     GGVQSRIAGPTVDFTSFFSTRGVLAAPLNATEQAGAAAFFISYQVATFANAAGWSNAP
                     AGDDNVAPPQQTVLTVATSFAAQNPTRLYDIYVYDAIDNDIPAYDTVLLVRSAAAKDA
                     NQASTHLQVGDFNEIKLSGPDGLIGDRAGQTAGFYTKLIALAPDLSSFKIYFTSVQRI
                     IATCSTAACNALPAGGLGENKLEKYLAEHFPTYIAGDFAPLEARIIDEDTYVEQGNDL
                     HDAYADAVTSYILGTLQPNTDVAFVGFPTTDEVQHQFLALTVPTDIDGDPNPYYDDLE
                     GNGTPDGLLTKRRGYLRDAYADADRALGQARALLGGNPTTFVGSDHGFAPQWYAVNAR
                     RVLFDASVDGISLQASGGNTASNCGATNIVLNPAPPAPAPNTYVSGDLTKACFAGGTI
                     QIYVNPTLPTGTTYAEIRAAVVAAFEGLTDPANPGEQVVEDVMLKEELRNVQGVDALH
                     PSRSGDVVVVLRPPYQSDAATPGVRIAFSQFFGQHGYLPDLVDIPHDINMHSTFIAGG
                     PGIRKQGPVAGVRAIDLAPTLAFLLNIPGPMNARGRIMYNLTKGAGSFKEITILDISD
                     YHGQLVPLFETADDLAAPAVNPQYTIGGSAFLDSWFDWFRAEAPNGSLTMAAGDSVGA
                     TPPISSFFGDTPTMELMNLMGIDLDGLGNHNFDAGSAYFRNTLVPLADFPFLSANVVD
                     NANKTPAEWKKSIVLNTFGGVKVGFVGFTNDDAPTLVAPGAFDPFHVSNSTTAVQAEV
                     NALKTKKVDVIVAFGHLGATAGTLTDPTGPLVDLADALTGVDAVVGDHTNQQAVDYRN
                     GVLVTENLSKGARFMRIRMVWDSSAKKVVYKTADFHKPWTIGVTPDPAIQTEIDALNT
                     QLQPLLGVVIGESTVDITRADACGRSDGRLCESLVGDVVTDAMRTAYTSIGVEFAITN
                     SGGLRDRLTCTGLGAGTGFCPTFTPPPYKITRGSVLAVLPFGNQVVTLPVNGAELKSI
                     LEVGVSTATNPSAPFGLAANGRFPEVSGLCFTYNIEAASGSRVTGAVRQAANGSCTGA
                     AIDLTAGSSYKIAWNDFNVAGGDGYPNFKARSSSQAILAQVVDDYISAAGSISPVIQG
                     RIHCFDPNPATGAACTTGSP"
     gene            complement(3954..>5363)
                     /locus_tag="VIF63_02735"
     CDS             complement(3954..>5363)
                     /locus_tag="VIF63_02735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY8238326.1"
                     /translation="RTFHALGLEMLRQAGEPVEPLLDRDEILRAVRPDASIAERRRFD
                     TVLSRFKLDLDIDPADIERDPDAGPVARTFAAYEAEIRRRGGLDFDDLVAGALRLLAE
                     RPEVLDAWRERCRHLLVDEAQDLDRAQLRMALLLAAPANRIFLVGDDDQSIYGWRLAD
                     VRRLLSLADAALPGLTRVDLETNYRCPAPVLERAVRLVEHNVERFAKVIRAGPKAEGR
                     LILAPSAADDVDRAAAVLASWPADGGTRAVLARTNRELLPVVAAAMRLSLPFRAAELV
                     LPIESPIVDELLNETRAVAEPGYAAPTDAALLPILALGRLPDRRTTAPVQASADEDEA
                     ASSKERREIAAALLAWAAATRTLDELAAAIEAARRQLDALRRDDAPLLLATAHGTKGL
                     EFDHVAVIGLDEGRFPSARSVAEAVEPERALEEERRLAYVAWTRARRSLTLVYDPGAP
                     SPFLLEAFSPAELAVSAPR"
BASE COUNT          885 a         1956 c         1747 g          775 t
ORIGIN      
        1 actcgtacgc gctcgcaccc gcatcgcggc cgtcctcgcc gcgctcacgc tgctcacgac
       61 gtcgctggtc atacccgcca gcgctgcgaa accgccggcg cccggagcac ttgccacgaa
      121 ggccctcttc tacgcggccg acggcatgcg cccggacaag gtcgacgcgt atgccgccca
      181 gggcgtcatg cccaacatga aggcgctggc ggcagcgggc gtcaagggcg tcaacggcat
      241 gcagccgggc ttcccgccga acaccggcgt gggctggtac acgatgatga ccggtacctg
      301 gccgagcgag catggctcga cgaacaacac ctaccaccga accggtgaag gcaacttcaa
      361 caaccgcaca agcttcagcg cgctccaggg cgcagcgttc gatcagccca cgatccaggc
      421 cgacaccctt gccgcggccg ccgagcgggc tggcaagaag gtcgcccaga tcgactgggt
      481 cggcggcgtc cagtccagga ttgccggccc gacggtcgac ttcacgagct tcttctcgac
      541 acgcggcgtc ctcgccgcgc ccctgaacgc aacggagcag gcaggcgctg cggccttctt
      601 catcagctac caggtcgcca cgttcgcgaa cgccgccggt tggagcaatg cccccgcagg
      661 cgacgacaac gtcgcgccgc cacaacagac cgtcctgacc gtcgcgacat cgttcgcggc
      721 ccagaacccg acccggctct acgacatcta tgtgtatgac gccatcgaca acgacatccc
      781 ggcatacgac accgtgctgc tcgtgcgctc ggcggcagcc aaggatgcga accaggcttc
      841 gacccacctc caggtcggcg acttcaacga gatcaagctc agcggccctg acggcctgat
      901 cggcgatcgt gccgggcaga ccgccggctt ctacacgaag ctgatcgccc tcgcgcccga
      961 cctcagcagc ttcaagatct acttcacgtc cgtgcagcgc atcatcgcga cgtgctcgac
     1021 cgcggcgtgc aacgcgctgc cggccggcgg cctcggcgag aacaagctgg agaagtacct
     1081 cgccgagcac ttcccgacgt acatcgccgg tgacttcgct cccctcgagg cccggatcat
     1141 cgacgaggac acctacgtcg agcagggcaa cgacctgcac gacgcctatg ccgacgccgt
     1201 cacgagctac atcctcggga cgctccagcc gaacaccgat gttgcgttcg tcggcttccc
     1261 gacgaccgac gaggtgcagc accagttcct ggcgctcacg gtgccgaccg acatcgacgg
     1321 cgatcccaac ccgtactacg acgacctgga aggcaacggc acgcccgatg ggctgctgac
     1381 gaagcgccgg ggctaccttc gcgacgccta cgccgacgcc gatcgcgcgc tcggccaggc
     1441 acgcgccctg ctgggcggta acccgacgac gttcgtcggc tccgaccacg gcttcgcccc
     1501 gcagtggtac gccgtgaacg cgcgcagggt cctgttcgac gcctcggttg acgggatctc
     1561 cctgcaggcg agcggcggca atacggcatc gaactgcggc gccacgaaca tcgtcctcaa
     1621 cccagcgccg cctgcacccg cgcccaacac ctacgtgtcc ggcgacctca ccaaggcgtg
     1681 cttcgccggc ggcacgatcc agatctatgt gaatccgaca ctcccgaccg ggaccacgta
     1741 cgccgagatc cgcgcggcgg tcgtcgccgc gttcgagggc ctcacggatc cggcgaaccc
     1801 cggcgagcag gtcgtcgagg acgtcatgct caaggaggag ctgcgcaacg tccagggcgt
     1861 cgatgcgctc cacccgagcc gcagcggcga cgtcgtcgtc gtcctgcgac cgccctacca
     1921 gtcagacgcg gcgacgccgg gcgtccggat cgcattcagc cagttcttcg gccagcacgg
     1981 ctacctgccc gacctggtcg acatccccca cgacatcaac atgcattcga cgttcatcgc
     2041 cggcgggccc ggcatccgca agcagggtcc ggtcgccggc gtgcgagcga tcgacctcgc
     2101 accgacgctc gcgttcctgc tgaacatccc cggcccgatg aacgcgcgcg gccggatcat
     2161 gtacaacctc accaagggcg cgggatcgtt caaggagatc acgatcctcg acatcagcga
     2221 ctaccacggt cagctggtgc ccctcttcga gacggccgac gatctcgcgg ccccggccgt
     2281 gaacccgcag tacacgatcg gtggctcggc gttcctcgac agctggttcg actggttccg
     2341 ggccgaggcg ccgaatggct ccctgaccat ggccgccggc gactcggttg gtgccacacc
     2401 gccgatctcg tcgttcttcg gtgacacgcc gacgatggag ctcatgaacc tcatgggcat
     2461 cgacctcgac ggcctcggca accacaactt cgatgccggc tctgcgtact tccggaacac
     2521 gctcgtgccg ctggccgact tcccgttcct gtcggcgaac gtcgtcgaca acgccaacaa
     2581 gacgccggcg gagtggaaga agtcgatcgt cctcaacacc ttcgggggcg tcaaggtcgg
     2641 cttcgtcggg ttcaccaacg acgacgcacc gaccctcgtt gcccccggtg ccttcgatcc
     2701 tttccacgtc tcgaactcga ccacggccgt ccaggccgag gtcaatgcgc tgaagaccaa
     2761 gaaggtcgac gtcatcgtcg cgttcggcca cctcggcgcg accgccggga cattgaccga
     2821 cccgacgggt ccgctcgtcg acctcgccga cgcgctgacc ggcgtcgacg ccgtcgtcgg
     2881 cgatcacacg aaccagcagg ctgtcgacta ccggaatggc gtcctggtga ccgagaacct
     2941 gagcaagggt gcgcgcttca tgcgcatccg gatggtctgg gactccagtg ccaagaaggt
     3001 cgtctacaag acggccgatt tccacaagcc gtggacgatc ggcgtcacgc ccgacccggc
     3061 catccagacc gagatcgacg ccctcaacac gcagctccag ccgctcctcg gcgtcgtgat
     3121 cggcgagtcc acggtcgaca tcacccgagc cgatgcctgc ggccgctccg atggccggct
     3181 gtgcgagtcg ctcgtcggtg acgtggtcac ggacgcgatg cgcacggcgt acacgtcgat
     3241 cggcgtcgag ttcgcgatca ccaactcggg cggcctccgt gaccgcctga cctgcaccgg
     3301 cctgggcgct ggaacgggct tctgcccgac ctttacgccg ccgccgtaca agatcacccg
     3361 gggctctgtg ctcgccgtgc tgcccttcgg caaccaggtg gtcaccctgc cggtgaacgg
     3421 cgcggagctg aagtcgatcc tcgaggtcgg tgtctccacc gccacgaatc cttcggcgcc
     3481 attcggcctc gccgccaacg gccgcttccc ggaggtctcc ggcctgtgct tcacgtacaa
     3541 catcgaggct gcctccggca gccgcgtcac cggcgcggtg cgccaggccg ccaacggctc
     3601 gtgcaccgga gcggcgatcg acctcacggc tggttcgagc tacaagatcg cgtggaatga
     3661 cttcaacgtc gccggcggcg acggctaccc gaacttcaag gcccggagct ccagccaggc
     3721 catcctggcc caggtcgtgg acgactacat ctccgccgct gggtccatca gcccggtgat
     3781 ccagggccgc atccattgct tcgatccgaa ccccgccacc ggcgccgcct gcacgacagg
     3841 ctcgccgtag ccgacacgat ccgccggacc ccggcggtgg agcgaacagc gcgcgagggc
     3901 gggccaccgg ctcgccctcg ttgattccct gcgggccttc gcgacgatcg atgtcagcgc
     3961 ggagcggaga ccgcgagctc cgcgggcgag aacgcctcga gcaggaacgg cgacggtgcg
     4021 ccggggtcgt acacgagcgt gagcgagcgc cgcgcccggg tccacgccac gtaggcgagg
     4081 cgccgctcct cctcgagggc gcgctccggc tccacggcct cggcgaccga cctggcgctg
     4141 gggaagcgac cttcgtcgag gccgatcacg gcgacgtgat cgaactcgag gcccttcgtg
     4201 ccgtgggcgg tcgcgaggag gaggggagcg tcgtcgcggc gaagcgcatc gagctggcgg
     4261 cgcgctgcct cgattgccgc ggcgagctcg tcgagcgtcc gcgtggccgc tgcccaggcg
     4321 aggagtgctg ctgcgatctc gcgccgctct ttcgagctcg ctgcctcgtc ctcgtcggcc
     4381 gaggcctgga ccggggcggt cgttcgccga tccgggagac ggccaagggc aaggatcggg
     4441 aggagggcgg cgtcggtcgg agctgcataa ccgggctccg cgaccgcgcg tgtctcattg
     4501 aggagctcat cgacgattgg cgattcgatc gggagcacca gctcggcggc ccggaacggc
     4561 agggacagcc gcatcgccgc ggcaacgact ggcagcagct cgcggttcgt ccgagcgagg
     4621 acggcacgcg tcccaccatc ggccggccag gaggcgagga ccgcggcggc ccggtcgaca
     4681 tcgtccgccg ccgacggcgc aaggatcagc cggccctcgg ctttcggtcc cgcccggatg
     4741 accttggcga agcgttcgac gttgtgctcg accaggcgta cggcgcgctc gagcaccggc
     4801 gccgggcagc ggtagttcgt ctccaggtcg acccgggtga gacccggcag ggccgcatcg
     4861 gcgaggctga ggagccgccg cacatccgcg agccgccagc cgtagatcga ctggtcgtcg
     4921 tcgccgacga ggaagatccg gttcgcgggc gcagcgagca ggagcgccat ccgcagctgc
     4981 gcccggtcga ggtcctgcgc ctcgtcgacg aggaggtgcc ggcagcgctc ccgccacgca
     5041 tcgagcacct cgggtcgctc ggcgaggagc cgaagggccc cggccacgag gtcgtcgaag
     5101 tcgaggccgc ctcgacgtcg gatctccgct tcgtacgcgg cgaacgtgcg ggcaaccggc
     5161 ccggcatcgg ggtcgcgctc gatgtcggct ggatcgatgt caaggtccag cttgaagcgc
     5221 gagagcacgg tgtcgaagcg gcgccgctcg gcgatgctcg cgtccggcct gaccgcgcgc
     5281 aggatctcgt cgcgatcgag gagcggctcg acgggctcac cggcctgtcg gagcatctcg
     5341 aggccgaggg cgtggaacgt ccg
//
LOCUS       DATBSW010000099         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1135722, whole genome shotgun sequence.
ACCESSION   DATBSW010000099 DATBSW010000000
VERSION     DATBSW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135722"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="cdaA"
                     /locus_tag="VIF63_02740"
     CDS             complement(<1..851)
                     /gene="cdaA"
                     /locus_tag="VIF63_02740"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY8238327.1"
                     /translation="MPQLLASILDQVRPETFLDIGITALLIYWVFSLIRGTSAVRLVI
                     GVSLVFAVYFAARTLNLLLLTQILETGAVVGLFALVVVFQPELRRALDRIGRVGSLGW
                     LVSAEDRAAEMIATEVARAAAVLSAESHGALIVIERETGLEQIAETGVMIHGDLSADL
                     LRTIFSPKSPLHDGAVIVRGSKVLAAGALLPLGETTIHHERFGTRHRAALGTTEQSDA
                     IVVVVSEENGHVSLVQRARIVRNLNENQLAQALRRLLEPSPERRRLWLPAGMAGAGGP
                     RLSDIGRA"
     gene            complement(925..2229)
                     /locus_tag="VIF63_02745"
     CDS             complement(925..2229)
                     /locus_tag="VIF63_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238328.1"
                     /translation="MTDSLFEQYKAALRRGHLAALAGDLDGALEAYRTAARLVPERSL
                     PVASQGTVLHRLDRWPEAAEAFDLALRMSPDDEAALRARATARAERGLRSGAAADFER
                     LAFVLDVASRSAEAAEAARRAAELDPSTSRELLADRLRIAAAHVADMLPPAAGAASTP
                     DSTDASDAAMARMRDAVGGADTAPAAQDVDAGAAEAASFEAAADLEAISARLHRFVDT
                     DAFATEPASESPTEPIEPEPVDAEPIDAEPMDAELVDDGPIGEQAAALAARLAELGTQ
                     PAWVDKPGRSVGAHAADGGTGVWPLIDLPSPPPPPIEGPPPDPETLLAEAADCIAAGD
                     LAGARDRMLVAAIVHRSAGRLDAALEICLQLLTTAPGDPHVHLAIANLQLDRGWTDLA
                     TEKIALLRRLTSLTGDTQAEADVHGLASERLRDDAASSAHVR"
     gene            complement(2288..2686)
                     /gene="rpsI"
                     /locus_tag="VIF63_02750"
     CDS             complement(2288..2686)
                     /gene="rpsI"
                     /locus_tag="VIF63_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8238329.1"
                     /translation="MATTPIFNSGTGRRKTAVARVRLLPGEGEMVINGRSFVEHFGAA
                     IDEAEVRMPFRVTNTDGRFNAMIKVEGGGVNGQAGAVRHGIARALLEFDESHRLLLRQ
                     AGFLTRDPRMKERKKYGLKRARKAPQYTKR"
     gene            complement(2700..3131)
                     /gene="rplM"
                     /locus_tag="VIF63_02755"
     CDS             complement(2700..3131)
                     /gene="rplM"
                     /locus_tag="VIF63_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY8238330.1"
                     /translation="MNDKTYTARGGDIERQWFVVDATDQTLGRLATRVARVLGGKHKP
                     TWSPNLDGGDHVIVLNAKKVSVSSAKLETKFYQRHSGHPQGFKEESLGKLLERRPEEV
                     IRRAVKGMMPHTRLGAQQLRKLKIYAGSDHPHQAQQPEPLT"
     gene            complement(3131..3937)
                     /gene="truA"
                     /locus_tag="VIF63_02760"
     CDS             complement(3131..3937)
                     /gene="truA"
                     /locus_tag="VIF63_02760"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8238331.1"
                     /translation="MPERARRGRTARRFRATVEYDGTEFFGFQAQPDARTVQGELEAA
                     LARLSGGIRQPVMGAGRTDAGVHATGQVIAFTYPGSLSVEALHEALNGTLPPDVAISG
                     LRRAPAGFNPRYAARYREYRYTIWNGPRSPLRERTSLWVRSGLDVTAMAVAATAFEGR
                     HDFSAFGGADPQPVRTVHRLRVRRQGSTVTIDVRADAFLRGMVRRIVAALLEVGRGQL
                     EASAVAGLLTAGKPALAGAAAPARGLCLRRVVLGRRQATTTSDMEPGHED"
     gene            complement(3940..>4076)
                     /locus_tag="VIF63_02765"
     CDS             complement(3940..>4076)
                     /locus_tag="VIF63_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HEY8238332.1"
                     /translation="PLVVTKLFDEIGPKYADRTSGFTRIVKLGPRAGDAAEIVQIELV
                     "
BASE COUNT          556 a         1414 c         1493 g          613 t
ORIGIN      
        1 gcgcgcccga tgtcggagag ccgtggcccg cccgcgccgg ccatcccggc cggcagccac
       61 aggcggcgcc gttccgggct cggttcgagc agccttcgaa gcgcctgggc gagctggttc
      121 tcgttgaggt tgcgcacgat ccgcgcccgc tggacgaggc tgacgtggcc gttctcctcg
      181 gacacgacga ccacgatcgc gtcgctctgc tcggtcgtgc cgagggccgc ccggtggcgc
      241 gtgccgaacc gctcgtggtg gatcgtcgtc tcgcccagcg ggaggagggc gccggcggcg
      301 agcaccttcg aaccgcgcac gatcaccgcg ccgtcgtgga gcggtgactt gggcgagaag
      361 atcgtccgga gcaggtcggc ggagaggtcg ccgtggatca tcaccccggt ctcggcgatc
      421 tgctcgagtc cggtctcgcg ctcgatgacg atcagcgcgc cgtggctctc cgccgagagg
      481 acggccgcgg ctcgggcgac ctcggtggcg atcatctcgg ctgcgcgatc ctccgcggag
      541 acgagccagc ccagcgaccc gacccgcccg atgcggtcga gggcccgccg gagctccggc
      601 tggaacacga ccacgagcgc gaacaggccg acgacggcgc ccgtctcgag gatctgggtg
      661 agcagcagca ggttgagcgt gcgggcggcg aagtacaccg cgaacacgag gctgacgccg
      721 atgacgaggc gcacggcgct cgttccgcga atgaggctga agacccagta gatgagaagc
      781 gccgtgatcc cgatgtccag gaacgtctct ggacgaacct gatccaggat cgacgcaagg
      841 agctgcggca tgctcggcga gtgtaccgcg cgagttccgc cggacggcct cgacgcctcg
      901 ggtcagcgcc cggccgacgg cccgctagcg aacgtgggcg gagctcgccg cgtcgtcccg
      961 gagccgctcg gaggcgagcc cgtggacgtc ggcctcggcc tgcgtgtcgc cggtcagcga
     1021 cgtcaggcgg cggagcagcg cgatcttctc ggtggcgagg tccgtccagc cgcgatccag
     1081 ctggagattg gcgatcgcga ggtgcacgtg cgggtcaccg ggcgcggtcg tgaggagctg
     1141 gaggcagatt tcgagcgccg cgtcgagccg gcctgcggac cggtggacga tggccgcgac
     1201 gagcatccga tcgcgcgcgc cggcgaggtc gcccgccgcg atgcagtccg ccgcctcggc
     1261 gagcagggtc tccgggtcgg gcggtggccc ctcgatcggt ggcggcggcg gggacggcag
     1321 gtcgatcagc ggccacacgc cggtgccgcc atcggcggcg tgcgctccga ccgagcggcc
     1381 cggcttgtcg acccacgcgg gctgcgtccc gagctccgca aggcgagcgg cgagcgccgc
     1441 ggcctgctca ccgatcggcc catcgtcgac cagctcggcg tccatcggct cggcatcgat
     1501 cggctcggcg tccaccggtt cgggctcgat cggctcggtc ggggattccg aggcgggttc
     1561 ggtcgcaaac gcgtcggtgt cgacaaaccg atggagacgc gcgctgatcg cctccaggtc
     1621 tgcagcagct tcgaatgacg ccgcttcggc tgcgcccgcg tcgacatcct gggccgccgg
     1681 cgccgtgtcg gctccaccaa ccgcatcgcg catgcgcgcc attgcggcat cggaggcgtc
     1741 ggtcgaatcc ggtgtgctcg ccgcgcccgc ggcgggcggg agcatgtccg cgacatgcgc
     1801 cgcggcgatg cgcaggcgat cggccagcag ctcgcggctc gtcgaaggat cgagctcggc
     1861 ggcgcgacgg gcagcctccg cggcctccgc ggatcggctc gcgacgtcga gtacgaaggc
     1921 aaggcgctcg aagtcggccg ccgcacccga gcggagccca cgttcggcgc gcgcggtcgc
     1981 gcgagcgcga agcgctgcct cgtcgtcggg tgacatgcgc agcgcgaggt cgaacgcctc
     2041 ggcggcttcg ggccagcggt cgagccggtg caggaccgtg ccctggctcg ccaccggaag
     2101 cgaccgctcg ggcacgagtc gggccgcggt tcgatacgcc tcgagggccc cgtcgaggtc
     2161 gccggcaagt gccgcgagat gcccgcgtcg aagcgccgcc ttgtactgct cgaagaggga
     2221 atcggtcacg tcggcgagcc tatcgcgccg cgggttcggg cggacccagc ccgaggtact
     2281 gcgccggtca gcgcttggtg tactggggcg ccttgcgtgc tcgcttgagg ccgtacttct
     2341 tgcgctcctt catccgcgga tcgcgggtca ggaagccggc ctggcggagc aggagtcggt
     2401 ggctctcgtc gaactcgagc agcgcccggg cgatgccgtg gcgcacggcg ccggcctggc
     2461 cgttcacgcc gccgccctcg accttgatca tcgcgttgaa gcggccatcg gtgttcgtga
     2521 cgcggaacgg catccggacc tcggcctcgt cgatcgcggc gccgaagtgc tcgacgaacg
     2581 agcgcccgtt gatgaccatc tcgccttcgc ccggcaggag ccggacacga gcgacggccg
     2641 tcttgcgacg accggtgccg gagttgaaga tcggggtcgt ggccatgcag gctcctccgt
     2701 caggtcagcg gttcaggctg ctgggcctga tggggatggt cggatccggc gtagatcttg
     2761 agcttgcgga gctgctgggc gccgagtcgg gtgtggggca tcatgccctt caccgcgcgc
     2821 cggatgacct cctcgggacg ccgctcgagg agcttgccca gcgactcttc cttgaacccc
     2881 tggggatggc cgctgtggcg ctggtagaac ttcgtctcga gcttcgccga agagacggag
     2941 accttcttcg cgttcagcac gatcacgtgg tcgccaccat cgaggttcgg cgaccaggtc
     3001 ggcttgtgct tgccgccgag gacacgagcg acacgcgtgg cgagacggcc gagcgtctgg
     3061 tccgtcgcat ccacgacgaa ccactggcgc tcgatgtcgc cccctctcgc ggtgtacgtc
     3121 ttgtcgttca tcagtcctcg tgtcctggtt ccatatcgct tgtcgtcgtc gcttgtcgtc
     3181 gtccgagaac gacgcgccgg aggcagagcc ccctggccgg tgccgcggcc ccggccagcg
     3241 ccggcttccc ggctgtgagc aggcctgcca cggcggaggc ctccagctgt cccctcccca
     3301 cctcgagcag cgctgcgacg atccgtcgca ccatgccccg caggaaggcg tcagcccgca
     3361 cgtcgatcgt gaccgtcgat ccctgccgtc ggacccggag ccgatggacg gtccggaccg
     3421 gctgcgggtc ggctcccccg aaggcgctga agtcgtggcg gccctcgaag gccgtcgcgg
     3481 ctactgccat cgcggtgacg tccagccccg accgcaccca gagcgaggtt cgctctcgaa
     3541 gcgggctgcg cggtccgttc cagatggtgt accggtactc ccggtagcgg gccgcgtagc
     3601 gggggttgaa cccggcgggc gcccgtcgga ggccgctgat cgcgacatcc ggcggaagcg
     3661 tcccgttgag cgcctcgtga agggcctcga ccgagaggct gcccgggtag gtgaatgcga
     3721 tcacctggcc cgtggcatgc accccggcgt cggtccgtcc ggcgcccatg accggctggc
     3781 ggatcccacc tgacaggcgg gccagcgcgg cctcgagctc tccctggacg gtccgggcgt
     3841 caggctgggc ctgaaacccg aagaactccg tgccgtcata ttcaaccgtg gccctgaacc
     3901 ggcgagcggt ccgcccacgt cgtgccctct cgggcatcgc taaacgagtt cgatctggac
     3961 gatctccgcc gcgtcgccgg cccgagggcc gagcttgacg atgcgggtga agccagaggt
     4021 tcggtcggcg tacttggggc cgatctcgtc gaagagcttg gtgacgacga gcggct
//
LOCUS       DATBSW010000100        17363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_431133, whole genome shotgun sequence.
ACCESSION   DATBSW010000100 DATBSW010000000
VERSION     DATBSW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431133"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VIF63_02770"
     CDS             complement(<1..990)
                     /locus_tag="VIF63_02770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY8238333.1"
                     /translation="MTTKGSGETRRETDSMGAVEVPADHYWAAQTQRSLHHFAIGRDV
                     MPERIIRAFGILKLAAADVNAALGLISSDEHDGIVAAAREVADGRLPTEFPLRVWQTG
                     SGTQTNMNVNEVIAGRANEVATGARGGKAPIHPNDHVNRSQSSNDTFPTALHMAVAMA
                     LVEDLVPSVRALRDALDAKRAEFDGIVKIGRTHLQDAVPLTLGQEFSGYVAQLDGGLA
                     RIDWALPGLYELALGGTAVGTGINSHPEFAERVARRIAELTGLPFVTAPNKFAALAGQ
                     EATVFASGTLRALATSLMKIANDIRWLGSGPRAGLGELRLPENEPGSSIMPGKV"
     gene            1074..1997
                     /locus_tag="VIF63_02775"
     CDS             1074..1997
                     /locus_tag="VIF63_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY8238334.1"
                     /translation="MRRVLLWMARNRWLRDHLPRLWFAKRAVRRFMPGEKPESALDAA
                     VTFRVDGISSMFTRLGENITKLEEGDEVAEHYLGLMGDIRARDLDGEVSVKLTQLGYD
                     LDVDRTLEHCKRLADKAAETHKALWIDMEGSAYAAGTIEFYERLKATHPNTGLCLQSY
                     LRRTAADIQHLLPLDPKIRLVKGAYSEPVELAYQSRKEVDANYVALAVALLEGGTQPG
                     RKVRLGLGTHDVVLIEQIAEKAAAMGLPKSSFEVQMLYGIRMDQQRRLAGEGYLVRDL
                     IAYGEAWYPWYMRRLAERPANVIFALRQIIG"
     gene            2112..2441
                     /locus_tag="VIF63_02780"
     CDS             2112..2441
                     /locus_tag="VIF63_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238335.1"
                     /translation="MVRVRDAKPEELAPKPRKPKAMSPRQLAIKRREQAIEKVLNELG
                     AGPASWIKKIELEDNEKLVTIRAAVARQIASSGSTVNLGVRNGAIFLSRGPIPGGRGG
                     GRRKKNA"
     gene            complement(2493..4160)
                     /gene="betA"
                     /locus_tag="VIF63_02785"
     CDS             complement(2493..4160)
                     /gene="betA"
                     /locus_tag="VIF63_02785"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY8238336.1"
                     /translation="MADERFDVVIVGGGSAGCVLANRLSEDPGTRVLVLEAGRPDWRF
                     DVFLHMPAALAFPIGSSFYDWGYRSEPEPFLHGRRVYHARGKVLGGSSSINGMIFQRG
                     NALDYEHWATVPGMADWDYAHCLPYFKRMESCLAAAPDDPYRGHTGPLVLERGDASSP
                     LFGAFFEAVQEAGYTLTDDVNGFRQEGFGRFDRNIHRGRRLSAATAYLKPALKRRNLE
                     VRTSALVTEIMFDGLRATGVEYRAGAGHHRVHAGEVILAGGAINTPQLLQLSGVGDPE
                     LLRPLGIARRAELPGVGANLQDHLEVYVQHTSREPVSMQPHATELWRRPFIGAQWLFL
                     RGGPGATNHFEAGGFVRGDEDVAYPNLMFHFLPLAIRYDGTAPAGKHGYQVHVGPMYS
                     DARGSVRIRSTDPAEHPALVFNYLSTAQDRREWVEAITVARDILGQKAFERYDSGEVS
                     PGPAVQTDAQILDWVGHDAETALHPSCTARMGSDIDAGAVVDPATMRVHGTEGLRVVD
                     ASVFPFVTNANIYAPVMMVAEKAADLIRGDTALPPEPLPFYRHQPTP"
     gene            complement(4185..4748)
                     /locus_tag="VIF63_02790"
     CDS             complement(4185..4748)
                     /locus_tag="VIF63_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8238337.1"
                     /translation="MDVTIHAFPSRDREFVEFAQAAWAGLAAPQTTDALQHALRVRYP
                     AAVVTEQAELARHGEGPTVWYAFRTAAVGAPGPEPRPEKTGAWAILDNERRFLEVSPD
                     LARIAELPARQMVGNRVEDFSNPADPSIVDDIARLWDEFRSSGALASTLRFNYADGRP
                     RELGYRLVANVDGEGRHRLTVRVLPEA"
     gene            complement(4817..5584)
                     /locus_tag="VIF63_02795"
     CDS             complement(4817..5584)
                     /locus_tag="VIF63_02795"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8238338.1"
                     /translation="MTATRRSPLTLAVIAVVVLGTLGAAGGLAYLFLRAAPPPAVGIA
                     TGSPGSGGPAASPGAGASLPVGTIGPEGIDGTWTLDPSIGSYSDFTSSFVGYRVNETL
                     ADVGANTAVGRTPIVTGSLVLDGSTVTSVDITADLSHLRSDDDRRDGRLREQAIETGQ
                     FPLSTFKLTTPIALGSLPVDGQTIDATATGELTLHGVTKTVSVPIRATLSGDVVTVIG
                     SIEIQFDDYGIEQPTSFLVLSIEDHGTMELQLHFRRG"
     gene            complement(5581..6966)
                     /locus_tag="VIF63_02800"
     CDS             complement(5581..6966)
                     /locus_tag="VIF63_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8238339.1"
                     /translation="MAEDIAVAPADEAASETDARLVRGVRRRLVAWSGGSTLVVLLAL
                     GIALYAAVANTLAGASVGQLEARTGPWVAALQGQPGSGQPPPGFGFQPGRGNTFLFAF
                     DSEGKPVQLGREPVVVLSGMPEPGGLDAARGAADGRNVWAGQLDIQSATIPVRILTQR
                     VTYREDGQTYFLQALQDRSTEVDTLNALVTVLLVGGLVVVLVAVGFGAIYARRALVPI
                     RESLAGQRASLQRQREFAADASHELRTPLTVIRASVEHLRRNAERPVAEVGDALVDID
                     AEVEHLTGLVDDLLLLARSDSGAAALERVPLHLDDVAADAAASLAQPAAAAGVRVEVD
                     PEPTAAVGDPARLRQLVTILVDNAIRHSPHGSAVRVIVRPDGSDATVAVEDAGPGVAP
                     EDRPRVFDRFWRAPGAPAGGTGLGLAIAKWIAEHHDGSISVDRSAAGGARFEVRIPGP
                     PVPIPAPEAAS"
     gene            complement(6959..7636)
                     /locus_tag="VIF63_02805"
     CDS             complement(6959..7636)
                     /locus_tag="VIF63_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8238340.1"
                     /translation="MHVLLVEDDPRLLRVLRRLLEDDRHVVEVAPDGETALDLVDATD
                     GIEVVVLDVGLPRMNGIEVARRLRRDGRELAILMLTARDTVNDRVSGLDAGADDYLVK
                     PFAYEELSARLRALARRTETGARRPDPVLAAGPIALDERSRRVTVRGKPVDLSPREFS
                     LLECFLRHPGQSLTRDQLLDQAWPFSVAVTPNAVDAYVHYLRTKLGEAGRWIETVRGV
                     GYRLADG"
     gene            complement(7656..8234)
                     /locus_tag="VIF63_02810"
     CDS             complement(7656..8234)
                     /locus_tag="VIF63_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238341.1"
                     /translation="MDRNDMAETNPSNLPAGGPEPAVPTQPVPAARPKARPNGGGRLL
                     NYALAGAVVLAIAGVAFAAGRVTAPAAVTSGNFPAGNGGQFPFPSGGFPGGNRPGNGN
                     GGPGGLVSSGGLTLEGTVESISGTTLTLKTADGQTIQIALDETTAYHAQTDANAADVT
                     TGTKVQVRLDLRGQGGAGNGAGASANDVTVVP"
     gene            complement(8246..9436)
                     /locus_tag="VIF63_02815"
     CDS             complement(8246..9436)
                     /locus_tag="VIF63_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238342.1"
                     /translation="MSLIELIRLALSRLRRGRVRAALTMLGVIIGVASVVGLVSVGRG
                     TTARITDQLSSLGTNLLTVNPSGRFGGDTTLTVDDADAIAAIDGIAGVAPEIQASGVV
                     ATSATNTTTTSIVGTSAAYATVRAFDVWQGTFLTDVQVDQSLRSVVLGATVASDLGLG
                     ADAIGTTVSIEGIPFEIVGILQPKGGSGFNDPDSQVLVPLGTVSKYFVGGSGVRSIGL
                     SVADGAAMDGVRNEVAELLRVRHGIGADDSDDFQIFDQTQLLEAASSISGTLTLLLGG
                     IASIALIVGGIGIMNIMLVSVRERTREIGIRKALGAKGSDILSQFLIEALTLSLLGGF
                     IGVLAGLVVSAVIGRIAGWGFIFDPNTIAMATLFSLLVGVVFGVWPARQAARLDPVIA
                     LRYE"
     gene            complement(9433..10110)
                     /locus_tag="VIF63_02820"
     CDS             complement(9433..10110)
                     /locus_tag="VIF63_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238343.1"
                     /translation="MSLLRLDQVRRTYDMGAVQVEALRGVSMSIERGEMVAIVGPSGS
                     GKTTMMSILGCLDRPTSGTYELDGTDVGSLDDGELARIRSRTIGFVFQSYNLLPGTSA
                     LDNVATPLLYQGVPRKERLARAAAALERLGLGERLQHEPTELSGGEQQRVALARALVT
                     EPALLLADEPTGNLPQHQGEVVLQLFSDLHAAGHTVLLITHNPDVAKVAGRQIHLRDG
                     VIVEAAA"
     gene            complement(10107..11279)
                     /locus_tag="VIF63_02825"
     CDS             complement(10107..11279)
                     /locus_tag="VIF63_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8238344.1"
                     /translation="MRLKLLAIVVLLVVGGGALYAAIGGFAPTPTAATSLLTAVATVT
                     DVTDEIAATGSVAAATRYQLWFGTDAVASDGSAADDGSSSQGDPTASSVTWPVTEVKV
                     AAGDHVTKGDVLATADDVDLEARIADANRSAKSAAIQLTIAEENLDGADTTDTRRQAR
                     LALYNAQTSDANARADLAALKALRDATTLVAPADGIVTAVAIASGTDAPSGAAITLLS
                     SDVRIVTSVVESDVASIEVGQAATVSVSAIDASLRGTVSSIDPVGSGSGQNGVVAYAV
                     NVALDAPPAALRPGMSADITIVADSASGVLAVPSRALSGSAGNYTVRVVAADGTVSVK
                     PVQVGLVTSSLAEIKAGLQAGEQVVTGSSSAQNQATNGNGGPGAFPGGGTIIRGNP"
     gene            complement(11389..12150)
                     /locus_tag="VIF63_02830"
     CDS             complement(11389..12150)
                     /locus_tag="VIF63_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8238345.1"
                     /translation="MSILSAVVASTGTTAALLGAFGSGAAISSPAANGQTVNNVNGDD
                     ITDVVARARESVVTITADGVRSNGPFQVPTTGVGSGVILTTDGYILTNRHVVDESRSL
                     TVAFADGTELPARIVQISSTTDLALIKVDTTGLRAMPIGDSSTLSVGQTAIAIGSPLG
                     TYTETVTRGIVSGLDREITVTNQATRAETTLRGLIQTDAAINPGNSGGPLLDGAGNVI
                     GINTAVATSAEGLGFAIPIGAAADLIGLAAGGAAA"
     gene            complement(12660..12821)
                     /locus_tag="VIF63_02835"
     CDS             complement(12660..12821)
                     /locus_tag="VIF63_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238346.1"
                     /translation="MSPAPTKVTGLGGGFVGGGLVGGGEVGFAVGLAVGFGVGFAVGL
                     GVGVGAAVG"
     gene            12796..13587
                     /locus_tag="VIF63_02840"
     CDS             12796..13587
                     /locus_tag="VIF63_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8238347.1"
                     /translation="MTFVGAGDIADCNSTGDSQTATLLDDIAGTVFTLGDNAYEDGTL
                     AEFKDCYGPTWGRHKSRTHPAVGNHEYQTPNAAGYFTYFGAKAGDPDEGWYAYNLGAW
                     RIYALNSNCAEIGGCGDSSRQVAWLKSDLAGHPKACVLAYWHHPRYSSGMHGNNPNTD
                     GLWDALYDAGAELVLVGHDHDYERFAPLNRTGIPDAARGIVEFVVGTGGRVHYAFNAI
                     KSTSRAHNNTAWGVLRLRLSAGSWTSRFVPVAGRTFSDTSSGTCH"
     gene            complement(13622..14050)
                     /locus_tag="VIF63_02845"
     CDS             complement(13622..14050)
                     /locus_tag="VIF63_02845"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8238348.1"
                     /translation="MPEAERTITINQPPSAVFPVIADGTKAPLWRSGVLDVALVSGSG
                     VGAVYRQVVRGPGGRRIDADYEVTAFEPDQRLAFRAIAGPVRPTGEFELEGIGGATAL
                     TFRLRAELSGWKRLLMSRAVQSTMDAEMAALDRLRDLLEA"
     gene            complement(14077..14811)
                     /locus_tag="VIF63_02850"
     CDS             complement(14077..14811)
                     /locus_tag="VIF63_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8238349.1"
                     /translation="MHVEERTWSSPALGQDMTLKVYGHEGRPILAFPSQDGRYGDFEA
                     WGMIDACAGFIDAGRVRFIAVDSIDGQSWTNQSLSPADRGLRHDAYDRYLADEVAPFM
                     RDLTGRERAWTTGASMGGYHAANVLFRHPDLFDGCVAISGLYQLGLFIGDDGSEPVYM
                     NSPLRYLPNLDDPWYLDRIRAAKLAFVVGQGAWEDDMLADTRALRAVLAAKGIPAIVD
                     EWGHDVNHDWPWWRKMLPHYLERLGV"
     gene            complement(14822..16006)
                     /locus_tag="VIF63_02855"
     CDS             complement(14822..16006)
                     /locus_tag="VIF63_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY8238350.1"
                     /translation="MNIVFLSPHFPPPMWLYVQRLREAGATVLGIADVAYEALRPELR
                     DALTEYYRVDDLDSIDSLIRAVGHFTHRHGKIDRLESLNEYWLETDARLRSEFNIPGL
                     RVETIDRVKRKSVMKRIFERAGVRVARGRVLRPGGAPAARQFVQDVGYPIIAKPDIGV
                     GAARTYRIENDTDLDAFLGDRAAIDYILEEFLEGQLLSYDGLVDRNGEIVWASSLVYG
                     LTVLDSVRGADMYYWIDREIAPDLDELGRRIVGAFGVRERPFHFEFFRLPDGELAALE
                     VNMRQPGGLTVDMWNWSGDIDFYRAWAEVVVTGTTRLPAEKPFYVMWAGRKVGRTYQI
                     SHEEVLRRFRKYLIHHERVDDVFATALGSYGYILREPRLDPLLAMAGEILAKAPAEQA
                     KG"
     gene            complement(16045..>17363)
                     /locus_tag="VIF63_02860"
     CDS             complement(16045..>17363)
                     /locus_tag="VIF63_02860"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEY8238351.1"
                     /translation="DPTVVGLDLWLVLGATAAWSFAVSGVAVTHRTATSVAGLGARAV
                     EAAFVGLGWLLSRAGADGFLADATRNLGRFAPVARGALIAVPIIMLFTLLLTSADAVF
                     GHALDEALRFPFDLEDIVGRGVFSILAAALVAGPVALAAGAGASLPGFAPAAPARSPE
                     GTEATDGAEPPSADPRAQPRSGATETLVVLGAVDLLFALFAIVQVVFLFGGTDTLAAI
                     GMSYSDYARQGYFQLAGVVALAGLLLLGAHEVVGRSRAFLYAGSTLMVLTAVILASAA
                     LRLALYQGAYGWTELRFYVAASIGWLALCVALALGLLLRDRMRWLPHGLAMSAVAVTL
                     AVSVIGPQAYVIHENLARVLDPRLVPPDGHAGLDIDYGATLGDDAIPDLVAALAVVDN
                     FDRANLRALLEQRALELSMEAEAAGPLSWNLARERAREALSHLPGS"
BASE COUNT         2619 a         5958 c         6348 g         2438 t
ORIGIN      
        1 caccttgccg ggcatgatcg aggagccggg ctcgttctcc gggagccgca gctcacccag
       61 gccggcgcgc gggccggagc ccagccagcg gatgtcgttg gcgatcttca tgaggctcgt
      121 ggcgagggcg cgcagcgtgc ccgaggcgaa gacggtcgcc tcctggccgg cgagcgcggc
      181 gaacttgttg ggcgcggtga cgaacggcag gccggtgagc tcggcgatgc gccgtgcgac
      241 gcgctcggcg aactccgggt ggctgttgat gcccgtcccg acggcggttc cgccgagggc
      301 cagctcgtac agtccgggta gcgcccagtc gatgcgcgcc agcccgccgt cgagctgcgc
      361 gacatagccg ctgaactcct ggccaagcgt gagcggcacc gcgtcctgga ggtgcgtccg
      421 gccgatcttc acgatgccat cgaactcggc gcgcttggcg tcgagggcgt cgcgcagggc
      481 acgcaccgac ggcacgaggt cctcgacgag ggccatcgcg acggccatgt gcagggccgt
      541 cgggaacgtg tcattgctcg actggctgcg gttgacgtgg tcgttcgggt ggatcggcgc
      601 cttgccgccc cgcgctcccg tggcgacctc gttggcacga cccgcgatga cctcgttgac
      661 attcatgttc gtctgggtgc cggacccggt ctgccagacg cggagcggga actccgtggg
      721 caggcgaccg tcggccacct cgcgtgccgc ggcgacgatc ccgtcgtgct cgtcgctgct
      781 gatgaggccg agcgcggcgt tcacgtcggc ggcggcgagc ttgaggatac cgaaggcgcg
      841 gatgatccgc tcgggcatca cgtcgcggcc gatggcgaag tggtgcagtg agcgctgggt
      901 ctgcgccgcc cagtagtggt cggccgggac ctcgacggca cccatcgaat cggtctcgcg
      961 gcgggtctcg ccagagccct tcgtcgtcac gacctcagta tggccgagcg gcacggcggt
     1021 tgcggtcaaa tggcccccgc cgccctgcgg ccggcgcggt aggctgcgtg gtgatgcgac
     1081 gcgtgctgct ctggatggcg cggaaccgct ggctgcgcga tcacctgccc aggctgtggt
     1141 tcgcgaaacg tgccgtccgg cggttcatgc cgggcgagaa gcccgagagc gcgctcgacg
     1201 ccgcggtcac gttccgcgtc gacggcatct cgagcatgtt cacccgcctc ggcgagaaca
     1261 tcacgaagct cgaggagggc gacgaggtcg ccgagcacta cctcggcctg atgggcgaca
     1321 tccgcgcccg cgacctcgat ggcgaggtaa gcgtcaagct gacccagctc ggctacgacc
     1381 tcgacgtcga ccggaccctc gagcattgca agcgcctggc cgacaaggca gccgagaccc
     1441 acaaggcgct atggatcgac atggagggca gcgcctacgc cgccgggaca atcgagttct
     1501 atgagcggtt gaaggcgacc cacccgaaca ccggcctctg cctgcagtcg tatctccggc
     1561 gcaccgccgc cgatatccag cacctgctgc cgcttgatcc caagatccgg ctcgtcaagg
     1621 gcgcctactc ggagccggtc gagctcgcct accagtcccg caaggaggtc gacgcgaact
     1681 acgtggccct ggccgtggcg ctcctcgagg gcggaacgca gcccggccgc aaggttcgcc
     1741 tcgggctggg cacgcatgac gtcgtcctga tcgagcagat cgccgagaag gccgcggcga
     1801 tgggcctgcc caagtcgagc ttcgaggtcc agatgctgta cggaatccgc atggaccagc
     1861 aacgacggct ggccggcgag ggctacctcg tccgtgatct gatcgcctat ggcgaggcgt
     1921 ggtatccgtg gtacatgcga cggctcgccg agcgaccggc aaacgtcatc ttcgcgctgc
     1981 gccagatcat cggttagggc ggattgatac tgctcattgc cgagttttga tttcggggcg
     2041 tggatcgtta cgataacgcc tgcattcgtc acgatcggcc gctgatcgcg acatcaccca
     2101 ggaggccccc aatggtccgc gtccgtgatg ccaagcccga agagcttgca ccaaagcccc
     2161 gcaagcccaa ggcgatgagc ccgcgccagc tcgcaatcaa gcgacgcgag caggcaatcg
     2221 agaaagtgct caacgagctc ggggccggac ccgcctcgtg gatcaagaag atcgagctcg
     2281 aggacaacga gaagctcgtc acgattcgcg ccgccgtggc tcggcagatt gcttccagcg
     2341 gcagcaccgt caatctcggc gtgcgcaacg gcgcgatctt cctcagtcgc ggcccgatcc
     2401 cgggcggacg aggcggcggg cggcgcaaga agaacgcctg aggcaccgcg agcgcgcctg
     2461 atcctccacc cgccggtgac cctcgatggg ggtcagggcg tcggctggtg gcggtagaag
     2521 ggcagcggct cgggcggcag cgcggtatct ccgcggatca ggtcggccgc cttctcggcg
     2581 accatcatca ccggcgcata gatattcgcg ttggtgacga acgggaagac ggaggcgtcg
     2641 acgacgcgca ggccctccgt gccatggaca cgcatcgtgg ccgggtcgac caccgccccc
     2701 gcgtcgatgt cggagcccat gcgggcggtg cacgacgggt gaagcgcggt ctctgcgtcg
     2761 tgaccgaccc agtcgaggat ctgcgcgtcg gtctggaccg ccggaccggg tgagacctcg
     2821 cccgagtcgt agcgctcgaa ggccttctgg ccgaggatgt cccgcgcgac ggtgatcgct
     2881 tccacccatt cgcgccggtc ctgcgcggtc gacaggtagt tgaagacgag cgccggatgc
     2941 tcggccggat cagtcgagcg gatgcggacc gagccccggg catcggagta catcgggccg
     3001 acgtggacct ggtagccgtg cttgcccgcg ggcgccgtgc cgtcgtagcg gatcgcgagc
     3061 ggcaggaagt ggaacatcag gttgggatac gcgacgtcct cgtcgccgcg cacgaagccg
     3121 cctgcctcga agtggttggt cgcgcctggc ccgccgcgca ggaacagcca ctgcgccccg
     3181 atgaacggcc ggcgccagag ctccgtggcg tggggctgca tcgagaccgg ctcgcggctc
     3241 gtgtgctgga cgtacacctc caggtggtcc tgcaggttcg cgccgacgcc aggcagctct
     3301 gcgcgcctcg cgatccccag cggtcgcagg agctccgggt cccccacccc cgacagctgg
     3361 agcagctgcg gcgtattgat cgcgccgcca gcgaggatga cctcgcccgc gtgaacgcgg
     3421 tggtgcccgg ccccggcccg gtattcgacg cccgtcgcgc gcaggccgtc gaacatgatc
     3481 tcggtgacaa gcgccgaggt gcgcacctcg agattgcggc gtttgagcgc gggcttgagg
     3541 tacgcggtcg ccgcggacag gcgccggccc cggtggatgt tccggtcgaa ccggccgaag
     3601 ccctcctggc ggaagccgtt gacgtcgtcg gtcagcgtgt agcccgcctc ctggaccgcc
     3661 tcgaagaagg cgccgaacag cgggctcgag gcatcgccgc gctcgagcac caggggcccc
     3721 gtgtgccccc gatatggatc gtcgggcgcc gcggccaggc acgattccat gcgcttgaag
     3781 tacggcaggc agtgggcgta gtcccagtcg gccatgcccg gcaccgtggc ccagtgctcg
     3841 tagtcgaggg cattgccgcg ctggaagatc atcccgttga tcgatgacga gccgccgagc
     3901 accttgcccc gggcgtggta gacacgccgg ccgtggagga acggctccgg ctccgagcga
     3961 tagccccagt cgtagaagct gctgccgatc gggaacgcga gcgcggcagg catgtgcagg
     4021 aacacgtcga agcgccagtc cggccggccc gcctcgagga cgagcacgcg tgtaccggga
     4081 tcctcggaca gccggttcgc gagcacgcag ccggccgacc cgccgcccac gatcaccacg
     4141 tcgaaccgct cgtcagccat cgctggcgga ctgtaacgcg cccctcaggc ctccgggagg
     4201 acgcgcacgg tgagccggtg acggccctcg ccgtcgacgt tggcgacgag gcggtagccg
     4261 agctcccggg gccgcccgtc ggcatagttg aaccggagcg tggaggcgag cgcgccgctc
     4321 gaccggaact catcccacag gcgcgcgatg tcgtcgacga tcgatgggtc ggctgggttg
     4381 ctgaagtcct cgacccggtt gccgaccatc tgccgggcag gcagctcggc gatgcgcgcg
     4441 aggtccgggc tcacctcgag gaaccggcgc tcgttatcga ggatcgccca ggcgccggtc
     4501 ttctcgggcc tcggctccgg gccgggcgcg cccaccgccg cggttcggaa tgcgtaccag
     4561 acggtcgggc cctcgccgtg tcgcgcgagc tccgcctgct cggtcacgac cgcggccggg
     4621 taccggaccc ggagtgcgtg ctggagggcg tccgtcgtct ggggcgccgc caggccggcc
     4681 cacgccgcct gggcgaattc cacgaactcg cgatcccgcg acgggaacgc gtggatcgtc
     4741 acgtccacga cacgagcata ggcgcgacca gcgacgccct tgcgccctga gccggccgca
     4801 ggagcccggc gcgagctcag ccgcggcgga agtggagctg gagctccatg gtcccgtgat
     4861 cctcgatcga gaggacgagg aacgaggtcg gctgctcgat gccgtaatcg tcgaactgga
     4921 tctcgatcga gccgatgacg gtgacgacgt caccggagag cgtcgcccgg atcgggacag
     4981 acaccgtctt cgtgaccccg tgcagcgtga gctcgccggt tgccgtggcg tcgatcgtct
     5041 ggccgtcgac cgggagcgag ccaagcgcga tcggggtggt cagcttgaag gtcgagagcg
     5101 ggaactgccc ggtctcgatc gcctgctcgc gcaggcggcc gtcgcgccgg tcgtcgtcgc
     5161 tgcgcagatg cgacaggtcg gcggtgatgt cgacgctggt gacggtcgac ccgtccagga
     5221 cgagcgagcc ggtcacgatg ggcgtccggc ccacggcggt gttggcgccg acgtcggcga
     5281 gggtctcgtt gacccggtac ccgacgaatg acgaggtgaa gtcggagtac gaaccgatcg
     5341 agggatcgag cgtccacgtc ccatcgatgc cctccggccc gatcgtgccc accggcagcg
     5401 aggcgccggc gcccgggctt gccgccgggc cgcctgatcc cggcgatcct gtcgcgatgc
     5461 cgacggccgg cggcggggcc gcgcgcagga agagatacgc caggccaccc gccgcgccga
     5521 gggtgccgag cacgacgacg gcgatcacgg cgagggtgag gggcgagcgg cgggtcgcgg
     5581 tcacgaggcg gcctccggtg ctggaatcgg cacaggtggt ccgggaatcc ggacttcgaa
     5641 gcgggcgccg ccagcggcgg atcggtcgac ggagatggag ccgtcgtggt gctcggcgat
     5701 ccatttggca atcgcgaggc caaggccggt gccgccggcc ggggcgccgg gtgcgcgcca
     5761 gaaccggtcg aacacgcgcg gccggtcttc cggggccacg ccgggcccgg cgtcctcgac
     5821 cgcgaccgtg gcgtcactgc catccggccg cacgatcacc cggactgcgc tgccgtgggg
     5881 gctgtgacgg atcgcgttgt cgacgaggat cgtgacgagc tggcgcaggc gcgccgggtc
     5941 gccgaccgcg gcggtcggct ccgggtcgac ctccacgcgg acgcccgcgg ccgcggccgg
     6001 ctgggccagc gacgcggcag cgtcagcagc gacgtcatcg agatggagcg gcacgcgctc
     6061 gagcgcggcg gcgccggaat ccgagcgggc gaggagcagc aggtcgtcga cgaggccggt
     6121 gaggtgctcg acctcggcgt cgatgtcgac gagcgcgtca ccgacctcgg ccacgggccg
     6181 ctcggcattc cggcggaggt gctcgacgga ggcgcggatc acggtgagcg gcgtccgcag
     6241 ctcatggctg gcgtctgcgg cgaactcgcg ctggcgctgc agcgacgcgc gctggccggc
     6301 gagcgactcg cggatgggga cgagcgcccg gcgcgcgtag atcgcgccga atccaaccgc
     6361 caccagcacc accacgaggc ccccgacgag gaggaccgtc accagggcgt tgagcgtgtc
     6421 gacttcggtc gagcggtcct gcagcgcctg caggaaatag gtctggccgt cttcccggta
     6481 tgtgacgcgc tgggtcagga tcctgacggg aatcgtcgcg gactggatgt cgagctggcc
     6541 tgcccagacg ttcctgccgt ccgcggcgcc gcgcgctgca tcgaggccgc ccggttccgg
     6601 catgccgctg agcacgacga caggttcgcg gccgagctgg accggcttgc cctcgctatc
     6661 gaatgcaaac aggaacgtgt tgccgcggcc gggctggaac ccgaagccgg ggggtggctg
     6721 cccagacccg ggctggccct ggagagccgc gacccagggg ccggtccgcg cctcgagctg
     6781 gccgacgctc gcgccggcga gcgtgtttgc caccgcggcg tacagcgcga tgccgagcgc
     6841 gagcaggacg acgagcgtcg agccgccact ccaggcgacg agacgccgcc gaacgccccg
     6901 gacgagccgg gcgtcggtct ccgatgcggc ctcgtccgcc ggcgccacgg cgatgtcctc
     6961 agccatcggc cagccggtag ccgacgccgc ggaccgtctc gatccaacgg ccggcctcgc
     7021 cgagcttcgt gcgaaggtag tggacatagg cgtcgaccgc gttcggcgtg accgcgacgg
     7081 agaacggcca ggcctggtcg agcagctggt cgcgggtgag cgactgcccg gggtgccgta
     7141 ggaagcactc gagcagcgag aactcgcgcg ggctcaggtc gactggcttg cccctcaccg
     7201 tgacgcggcg gctccgctcg tcgagcgcga tcgggcccgc ggccaggacc gggtcgggcc
     7261 ggcgggcgcc ggtctcggtt cgccgtgcga gcgcccgcag ccgagccgag agctcctcgt
     7321 aggcaaatgg cttgacgagg tagtcgtcgg cgccggcatc gagcccgctg acgcgatcgt
     7381 tgacggtgtc ccgtgcggtc agcatcagga tcgccagctc gcggccgtca cgccgcaggc
     7441 gtcgggccac ttcgatgccg ttcatcctcg gcaggccgac gtcgaggacg accacctcga
     7501 tgccatcggt ggcatcgacc aggtcgagcg ccgtttcgcc gtcaggggcg acctccacga
     7561 cgtggcggtc gtcctcgagc agccggcgca ggacgcgcag gagccgcggg tcgtcctcga
     7621 cgagcaggac gtgcatggct gggatcgacg ctcccctacg ggacgacggt cacgtcgttc
     7681 gccgacgcgc ccgcaccgtt gccggcgccg ccctgtccgc gcagatcgag gcgaacctgc
     7741 accttggtgc cggtcgtgac gtccgcggcg ttcgcatcgg tctgggcatg gtaggcggtg
     7801 gtctcgtcga gggcgatctg gatcgtctgg ccgtcggcgg tcttcagcgt cagggtcgtg
     7861 ccgctgatcg actcgaccgt cccctcgagc gtgagcccgc ccgagctgac gaggccgccc
     7921 ggtccgccgt tgccgttgcc ggggcggttg ccgccgggaa agccgccgct cggaaacggg
     7981 aactggccgc cgttgcccgc ggggaagttg ccggaggtca cggccgctgg cgccgtcacc
     8041 cggccggcgg cgaacgccac accggcgatc gccaggacga cggctcccgc aagcgcgtag
     8101 ttgaggaggc gaccgccgcc gttggggcgc gccttcgggc gcgcggccgg aaccggctgc
     8161 gtcggaactg cgggctccgg gccgcccgcc ggcaggttgc tcgggttggt ctcggccatg
     8221 tcatttcggt ccacggttga ctccttcact cgtaacggag ggcgatgacg ggatcgaggc
     8281 gggcggcctg ccgggcaggc cagacgccga agacgacgcc gacgagcagg ctgaagagcg
     8341 tcgccatcgc gatggtgttg gggtcgaaga tgaagcccca gcccgcgatc cggccgatga
     8401 cggccgacac cacgaggccg gcgagcaccc cgatgaagcc gccgagcagt gacagcgtca
     8461 gggcctcgat gaggaactgg ctgagaatgt cgctgccctt ggcgccgagg gctttgcgaa
     8521 tgccgatctc gcgggtccgc tcccggaccg agaccagcat gatgttcatg atcccgatgc
     8581 caccgacgat cagggcgatc gaggcgatgc ctccgagcag gagggtgagg gtcccgctga
     8641 tggaggacgc cgcctcgagc agctgggtct ggtcgaagat ctggaaatcg tcgctgtcgt
     8701 cggcgccgat cccgtggcga acgcgcagga gctcggcgac ctcgttacgg acgccatcca
     8761 ttgccgcacc atcggcaacg ctcaggccga tcgatcggac cccgctgccg ccgacgaagt
     8821 acttcgagac ggtccccagc ggaacaagca cctggctgtc ggggtcgttg aaccccgagc
     8881 cacccttggg ttggaggatc ccgacgatct cgaacggaat gccttcgatc gagacggtcg
     8941 tcccgatcgc atcggcgcca aggccgaggt cgctggcaac cgtcgcgccg agcacgaccg
     9001 accggagcga ctggtcgacc tggacgtcgg tcaggaacgt gccctgccag acgtcgaagg
     9061 cacggaccgt ggcgtaggcc gcggaggtgc cgacgatcga ggtcgtcgtg gtgttggtag
     9121 cgctcgtggc aacgacgccg gacgcctgaa tctccggtgc gactccggcg atcccgtcga
     9181 tcgcggcgat cgcgtcagcg tcgtcgaccg tcaacgtggt atcgccaccg aaccggcccg
     9241 acgggttgac cgtcagcagg ttcgtgccga ggctgctgag ctggtcggtg atgcgcgcgg
     9301 tcgtgcccct gccgacgctc accaggccga ccacggacgc cacgccgatg atgacgccga
     9361 gcatcgtgag cgccgcgcgg acccggccgc gacgcaggcg tgagagtgcg agacggatca
     9421 gctcgatgag gctcatgccg ccgcctcgac gatcacgccg tcgcgcaggt ggatctgccg
     9481 gccggccacc ttcgcgacat ccgggttatg ggtgatgagc aggaccgtgt ggcctgcggc
     9541 gtggagatcg ctgaacaact gcaggacgac ttcgccctgg tgctggggaa ggttgccggt
     9601 gggctcatca gcgaggagga gcgcgggctc cgtcacgagc gcgcgcgcga gcgccactcg
     9661 ttgctgttcg ccacccgaca gctcggtcgg ctcatgctgg agccgctcgc cgaggccgag
     9721 tcgctccagc gccgctgccg cgcgggcgag ccgttccttc cgcggaacgc cctggtacag
     9781 caacggcgtc gcgacgttgt cgagggcgct ggtgcccgga agcaggttgt agctctggaa
     9841 gacgaacccg atggtccggc tgcggatccg ggcgagctcg ccgtcgtcga gcgagccgac
     9901 gtcggtgccg tcgagctcgt aggtgccgga ggtcggccgg tcgaggcagc cgaggatgct
     9961 catcatcgtc gtcttgcccg agccggacgg cccgacgatc gcgaccatct cgccgcgctc
    10021 gatggacatc gacacgccgc gaagggcctc gacctggacc gcgcccatgt cgtaggttcg
    10081 gcggacctga tcgagcctga ggaggctcac gggttgcccc ggatgatcgt gccgccgccc
    10141 gggaaggcgc cggggccgcc gttgccattc gtggcctggt tctgtgcgga gctcgagccg
    10201 gttacgacct gctcgcccgc ctggaggccg gccttgatct cggccaggga gctcgtcacg
    10261 agcccaacct ggacgggctt gaccgagacg gtgccgtcag cggcgacgac ccggaccgtg
    10321 tagttcccgg ccgagcccga aagcgcccgc gagggcacgg cgaggacgcc gctcgcgctg
    10381 tcggcgacga tggtgatgtc ggccgacatg cctggccgca gcgcggccgg cggcgcatcg
    10441 agcgcgacgt tgaccgcgta ggcgacgacg ccgttctggc cggagccaga gccgaccggg
    10501 tcgatcgagc tgacggtccc ccggagcgag gcgtcgatgg ccgagacgct cacggtcgcg
    10561 gcctgtccga cctcgatcga ggccacatcg ctctcgacga cgctggtgac gatccggacg
    10621 tccgaggaca gcagcgtgat cgccgcgccg ctcggggcgt cggtgccgct cgcgatggcg
    10681 acggcggtga cgatgccgtc cgcgggcgcg acgagggtgg tcgcgtcacg cagggccttc
    10741 agcgcagcga ggtcggccct ggcgttcgcg tcggaggtct gggcgttgta cagggcgagc
    10801 cgagcctggc gacgcgtgtc cgttgtgtcg gcgccgtcga ggttctcctc ggcgattgtc
    10861 agctggatgg cggccgactt ggcgctccgg ttcgcgtcgg cgatcctggc ctcgaggtcc
    10921 acgtcgtctg ccgtggcgag gacgtcgccc ttggtgacgt ggtcgccggc cgcgaccttg
    10981 acctcggtca cgggccaggt gactgacgac gccgtggggt cgccctggga gctgctgccg
    11041 tcatcggccg cggagccgtc gctggcgacg gcgtccgtgc cgaaccagag ctggtagcgc
    11101 gtggcggctg cgaccgatcc cgtcgcggcg atctcgtcgg tgacatcggt gaccgtggcc
    11161 acagcggtga gcaggctcgt cgctgcggtc ggcgtcggcg caaagccacc gatcgccgca
    11221 tacagtgcac cgccgccgac gaccaggagc accacgatcg cgaggagctt gaggcgcatg
    11281 aggtcccctc ccatgaagct ggcgtagcgc cggaccggcg ccgggttggc gaaggcgcgg
    11341 tcaccgtcag gcggccgcgc cctcgagggc ggtgttggtc gatgggcgct aggcggcagc
    11401 gccaccggcc gccaggccga tgagatccgc ggccgcgccg atcgggatcg cgaagccgag
    11461 gccctcggcg ctcgtcgcga cggcggtgtt gatgccgatc acgttgccgg ctccgtcgag
    11521 gagcgggcca ccgctgttgc cggggttgat cgcggcgtcg gtctggatca gcccacggag
    11581 cgtcgtttcg gcccgggtgg cctggttcgt gaccgtgatc tcgcgatcga gccccgacac
    11641 gatgccgcgg gtcaccgtct cggtgtacgt cccgagcggg ctgccgatcg cgatcgcggt
    11701 ctggccgacg gacagcgtgc tcgagtcacc gataggcatc gcccggagcc ccgtcgtatc
    11761 gaccttgatc agggcgaggt cggtcgtgct cgagatctgg acgatgcgcg ccggcagctc
    11821 ggtgccgtcg gcgaaggcga cggtgaggct gcggctttcg tcgacgacat ggcggttggt
    11881 caggatgtag ccgtcggtcg tgaggatcac gcccgagccg acgccggtcg tcggcacctg
    11941 gaatgggccg ttcgaccgca cgccgtcggc ggtgatggtc acgaccgatt cgcgcgcccg
    12001 agccaccacg tcggtgatgt cgtcgccgtt gacgttgttg acggtctggc cgttcgcagc
    12061 ggggctgctg atcgccgcgc ccgagccgaa tgcgccgagg agcgcggctg tcgtgccggt
    12121 cgacgcgacg accgcggaca ggatgctcac cgcaagaaga ccggcgaggc cccggccgcc
    12181 cgctcgggac gaccggggag ccgcgtcggc gaccgaagct tcgaaccggg gaggaagagc
    12241 cggttcgtcg tcggcctgaa ggattggggt gtcggaggag ggggtcccgg gtgcggtctc
    12301 gtcgttcatg gggccatcct cggccgggcc cctgagattc tcctgagatg gccccctggg
    12361 tcgaaagcaa agggagcgct acgctcgcac cgtggcaatg agagtggtcg cctgacgcga
    12421 cttccggcct cgccggccgt tcggttcgcg gcgctcgcgg tcggcctgct cctcgtcgtc
    12481 gtgatcgcga tcgccgcggg cgactggctc cttgcggaac caggcgcatc gaacggcccc
    12541 ggcgcgacga catcggcgct ggcgagtccc agcccggcgc ccggctcggc ctcgcccgca
    12601 gtcacgacga tcgggccgac ggccggacca tccccgagct ccgctgccac ggcagcggtt
    12661 cagccgacgg cggcaccgac accgacgccc aggccgaccg cgaagccaac cccgaagccg
    12721 actgccagac cgacagcgaa gcccacctcg ccgccgccga ccagtccccc gcccacgaac
    12781 ccgccgccca gccccgtgac cttcgtcggc gcgggcgaca tcgccgattg caactcgacc
    12841 ggcgactcgc aaaccgccac cctcctcgac gacatcgcgg gcaccgtgtt cacgctgggc
    12901 gacaacgcgt atgaagacgg aacgctcgcc gagttcaagg actgctacgg cccgacgtgg
    12961 ggccggcaca agagccggac gcacccggcg gtgggcaatc acgaatacca aaccccaaac
    13021 gccgcgggct acttcacgta cttcggggcg aaggccggcg atcccgacga gggttggtac
    13081 gcgtacaacc tcggcgcgtg gcggatctac gccctcaaca gcaactgcgc cgagatcggc
    13141 ggctgcggcg acagctcgag gcaggtcgcc tggctcaagt cagacctcgc cggccacccg
    13201 aaggcgtgcg tcctcgcgta ctggcaccac ccccggtaca gctccgggat gcatggcaac
    13261 aacccgaaca ccgacggcct gtgggacgcg ctgtacgacg cgggcgccga gctcgtcctc
    13321 gtcggccacg accacgacta cgagcgcttc gcgccgctca accgcactgg gatccccgac
    13381 gcggcacgcg gcatcgtcga gttcgtcgtc ggcacgggcg gccgggtgca ctacgcgttc
    13441 aatgcgatca agtcgacgag ccgcgcccac aacaacacgg cgtggggcgt cctcaggctc
    13501 aggctgtcgg ctggttcgtg gacctcgcgc ttcgtgccgg tcgcgggcag gacgttctcg
    13561 gacacgtcca gcggcacctg ccactgacgg ccggccgaat ccggccgcga tcaggcctcg
    13621 atcaggcctc gagcaggtcg cgcagcctgt cgagcgcggc catctccgcg tccatcgtcg
    13681 actggacggc gcggctcatg agcagtcgct tccagcccga cagctcggcc cgcaggcgga
    13741 acgtcagcgc ggtcgcgccg ccgatgccct cgagctcgaa ctcgccggtc ggccgaaccg
    13801 gaccggcgat cgcccggaac gcgaggcgtt gatccggctc gaacgccgtg acctcgtagt
    13861 ccgcgtcgat ccggcggccg cccggcccgc gcacgacctg gcgatacacc gcgccaacgc
    13921 cggagccgga gacgagcgcg acgtcgagca cgccggaccg ccacagcggc gccttcgtcc
    13981 cgtcggcgat gacggggaag acggccgacg gcggctggtt gatcgtgatc gtccgctcgg
    14041 cctcgggcat gacgcctcct cgtgcttcgg gcgagctcag accccgagcc gctccaggta
    14101 gtgcggcagc atcttgcgcc accacggcca gtcgtgattc acgtcgtggc cccactcgtc
    14161 gacgatcgcc gggatgccct tcgccgcgag cactgcgcgc agggctcgcg tgtccgcgag
    14221 catgtcgtct tcccacgcgc cctggccgac gacgaacgcg agcttcgctg cgcggatccg
    14281 gtcgaggtac cacgggtcgt cgaggttcgg caggtagcgc agcgggctgt tcatgtacac
    14341 cggctcgctg ccgtcgtcgc cgatgaacag cccgagctgg tacagccccg agatcgcgac
    14401 gcagccgtcg aacaggtccg gatgccggaa gagcacgttc gcggcgtggt agccgcccat
    14461 gctcgcaccg gtggtccagg cgcgttcccg gccggtcagg tcccgcatga acggtgcgac
    14521 ctcgtcggcg agataccggt cgtaggcatc gtggcgcagc ccgcggtcgg cgggcgagag
    14581 cgactggttc gtccatgact ggccgtcgat cgaatccacg gcgatgaacc gcacccggcc
    14641 cgcgtcgatg aagccggcac aggcatcgat catgccccac gcctcgaagt cgccgtagcg
    14701 gccatcctgc gacgggaacg cgaggatcgg ccggccttcg tggccgtaca ccttgagcgt
    14761 catgtcctgg cccagcgccg ggctcgacca ggttcgttcc tcgacgtgca cccgcggcgc
    14821 cctacccctt cgcctgctcg gcgggcgcct tggcgaggat ctcaccggcc atcgccagga
    14881 gcggatcgag gcgcggctcg cggaggatgt agccgtagct gccgagggcg gtcgcgaaca
    14941 cgtcgtcgac ccgctcgtgg tggatgaggt acttgcggaa ccggcgcagg acctcctcgt
    15001 gcgagatctg gtacgtccgg ccgaccttgc ggccggccca catcacgtag aacggcttct
    15061 cggccggcag gcgcgtcgtg ccggtgacga caacctcggc ccaggcccgg tagaagtcga
    15121 tgtcgcccga ccagttccac atgtcgaccg tcaggccgcc gggctggcgc atgttgacct
    15181 cgagcgccgc gagctcgccg tcggggaggc ggaagaactc gaagtggaac ggccgctcgc
    15241 gcacgccgaa cgcgccgacg atgcgccggc ccagctcgtc gaggtccggc gcgatctccc
    15301 gatcgatcca gtagtacatg tccgcgccgc gcaccgagtc gagcacggtc agcccgtaca
    15361 cgagcgacga cgcccacacg atctcgccgt tgcgatcgac gagcccgtcg tagctgagga
    15421 gctggccctc gaggaactcc tcgaggatgt agtcgatggc cgcgcggtcg ccgaggaagg
    15481 cgtcgaggtc ggtgtcgttc tcgatccggt aggtgcgggc ggcgccgacg ccgatgtcgg
    15541 gcttggcgat gatcgggtag ccgacgtcct gcacgaactg ccgcgccgcg ggtgcgccgc
    15601 cgggccggag gacgcggccg cgcgccaccc gcacgccggc ccgttcgaag atccgcttca
    15661 tgaccgactt gcgcttgacc cggtcgatcg tctcgacgcg cagccccggg atgttgaact
    15721 cgctgcgcag gcgggcgtcg gtctccagcc agtactcgtt gagcgattcg agccggtcga
    15781 tcttgccgtg gcggtgggtg aaatggccga ccgcacggat gagcgaatcg atcgagtcga
    15841 ggtcatcgac gcggtagtac tcggtgaggg cgtcgcggag ctcggggcga agcgcctcgt
    15901 atgccacgtc cgcgatgccg agcacggtcg cgcccgcctc ccgtagccgc tggacgtaca
    15961 gccacatcgg cggcgggaag tgcggcgaga ggaagacgat gttcacgcgc gcggcacgaa
    16021 ggcgatcacg cgcgagagtg tgccctagga ccccggcagg tgcgacagcg cctcgcgggc
    16081 gcgctcacgg gcgaggttcc aggacagggg gccggcagcc tccgcctcca tcgacagctc
    16141 cagcgcgcgc tgttcgagca acgcccgcag gttggcgcgg tcgaagttgt ccacgacggc
    16201 gagcgccgcc acgaggtccg ggatcgcgtc gtcgccgagg gtcgcgccgt agtcgatgtc
    16261 gaggccggcg tggccgtcgg gcggaacgag gcgtgggtcg agcacgcggg cgagattctc
    16321 gtggatcacg tacgcctgcg gcccgatgac cgagacggcc agcgtgacag ccacggcgct
    16381 catggcgagg ccatggggca gccagcgcat gcggtcgcgg agcaggaggc cgagggccag
    16441 ggcaacgcac agcgccagcc aaccgatcga cgcggccacg tagaagcgca gctcggtcca
    16501 gccgtaggca ccctggtaca gcgcaagtcg gagcgcggcc gaggccagga tcaccgcggt
    16561 caggaccatg agcgtgctcc cggcatagag gaatgcgcgg gaccggccga cgacctcgtg
    16621 ggcgccgagg agcagcaatc ccgccagcgc gacgacgccg gcgagctgga agtagccctg
    16681 gcgtgcgtag tcgctgtagg acatcccgat ggccgcgagg gtgtccgtgc cgccgaagag
    16741 gaagacgacc tggacgatcg cgaagagcgc gaagaggagg tcgaccgcac cgaggacgac
    16801 gagtgtctcg gtcgcgcccg aacgaggctg cgcgcggggg tcagcggacg gcggctcggc
    16861 gccgtcggtt gcctcggtcc cctcgggcga cctggctggc gcggcgggcg cgaagcccgg
    16921 cagggacgcg ccggcgcctg cggcgagcgc gaccgggccg gcgacgagcg cggccgcgag
    16981 gatcgagaag acgccgcgac caacgatgtc ctcgaggtcg aacgggaacc gcagcgcctc
    17041 gtcgagggcg tggccgaaca ccgcgtcggc agacgtgagc aggagcgtga acagcatgat
    17101 gatcgggacg gcgatgagcg cgccccgggc gaccggcgca aaccggccga ggttccgcgt
    17161 cgcatcggcg aggaagccgt cggcgccggc gcgcgaaagc agccacccga gcccgacgaa
    17221 cgcggcctcg acggcgcgcg cgcccaggcc ggcgaccgac gtggcggttc tgtgggtgac
    17281 cgcgaccccg ctgactgcga agctccacgc ggccgtcgcg ccgaggacga gccagaggtc
    17341 gagcccgacg accgttgggt ccg
//
LOCUS       DATBSW010000101         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_113857, whole genome shotgun sequence.
ACCESSION   DATBSW010000101 DATBSW010000000
VERSION     DATBSW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113857"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VIF63_02865"
     CDS             complement(<1..1208)
                     /locus_tag="VIF63_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238352.1"
                     /translation="MPSRPSAARKSSAVVDTPRDPSLSFAPLDRAIEREPRVDPDVEP
                     PKPDRASDPRSRRRRPRLIVEPGRPAIVGSIASTGGHLGLSEYPVQISKVQAPPLREE
                     TLARDRLLEWLSVKIHRRAVLLIAEAGYGKTTLLADFTRRTRIRVLWFRLDRGDRDWV
                     GFLAYLVAAVRIHVPDFGQATSALLRETATAAPPLETVLDAFLRELGGLTNDPTALVF
                     DDFHLVDDSPDVRHILRELLTRGPERMSFVFASRREPPVRLARLRALGEVAELSTNDL
                     RFDAGETERLFSETYEMPLEPAVLAELSRRTEGWAASLQLVRAALHDRNPGQVRAFIS
                     SLSGAEGHLYEYLAEEVVGDLPVDLQQFLMRTSVLDTIDLTLGPVAAEVSLDETRRFI
                     EDGERHGLFG"
     gene            complement(1311..1514)
                     /locus_tag="VIF63_02870"
     CDS             complement(1311..1514)
                     /locus_tag="VIF63_02870"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY8238353.1"
                     /translation="MGVIIVREMVGTSPKSWSDAARQAVATASRTVRNIKTVEVVHSS
                     ANVEDGEIVEYRVDIKIGFEYEG"
     gene            complement(1558..1869)
                     /locus_tag="VIF63_02875"
     CDS             complement(1558..1869)
                     /locus_tag="VIF63_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY8238354.1"
                     /translation="MYEDRTLTCRDCGDGFIFSGGEQRFFAEKGLQNVPQRCPGCRAN
                     AKRVRMGGPREYHAAVCNACGDQAMVPFAPRTDRPVYCSTCFDKVRAGVISPAAAPVS
                     S"
     gene            2082..2996
                     /locus_tag="VIF63_02880"
     CDS             2082..2996
                     /locus_tag="VIF63_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238355.1"
                     /translation="MTLRSARPVAVVARAAAAPILLAALIAACSTAAAPSPTPGATGS
                     PSAPPTAAPTSTPEIDGIQHPTGATDVVLRMEEGGGFVPIDFLATSAPQFTLYGDGTV
                     VFRDPTATLPEPVGGVTRSAPFLIVNIGEGGIQALLTEAIGPGGLGAAVGPYMGQGAD
                     MSTTTFTINADGDTKQVSVSGFLPDLHPQDAVIVGALGRLAERLSGFADAIAGEAKYV
                     PAAYRGILMPVDQPFGPVIAWPWTDITPAEFVPGDNEFFMTRTMTLAEVAVLEIPGAE
                     GGFLGLTLKHEGKLFSFSLRPLLPDETK"
     gene            complement(3031..>3935)
                     /gene="dnaB"
                     /locus_tag="VIF63_02885"
     CDS             complement(3031..>3935)
                     /gene="dnaB"
                     /locus_tag="VIF63_02885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY8238356.1"
                     /translation="ITTGFSHLKDLLHSAYDRLDYLHAHRGELSGISSGFLDLDQLTT
                     GFQKSDLVVLAARPSVGKTSFALNVAEHAAVKERKTVALFSLEMSKEQLVLRLLSSVA
                     NIDSKRLRSGFLEELDFARIAPAMNDLSEAALYIDDTPNITSMELRTKARRLQAESGL
                     DLVIVDYLQLMQATTQNRDSNRVQEVSEISRGLKALARELSVPVMALSQLSRQPEMRE
                     SKEPRLSDLRESGAIEQDADLVLFLYREKEKVGEEADLDGEVINLKLAKHRNGPTGDI
                     QLWFRKSQTRFQSYAGQRYEGAGV"
BASE COUNT          604 a         1338 c         1366 g          627 t
ORIGIN      
        1 ttgccgaaga ggccgtggcg ttctccgtcc tcgatgaagc ggcgcgtctc gtccaggctg
       61 acctcggccg cgacgggacc caaggtcaga tcgatcgtgt ccagcacgga cgtgcgcatc
      121 aggaactgct gcagatcgac cggcaggtcg ccgacgacct cctcggcgag gtactcgtag
      181 agatgccctt cggcgccgct cagggacgag atgaacgcgc gcacctggcc gggatttcga
      241 tcgtggagcg ctgcgcggac cagctgcagg gaagcggccc agccctcggt gcggcgactg
      301 agctcggcga ggactgccgg ctcgaggggc atctcgtagg tctcgctgaa gagtcgctcg
      361 gtctcgccgg catcgaaacg caggtcgttg gtgctgagct ccgcgacttc gccgagggct
      421 cggagacgag cgagtcggac cggcggctcg cggcggctcg cgaacacgaa ggacatccgc
      481 tcgggcccgc gggtcaggag ctcacgcagg atgtgccgca cgtcgggtga atcgtcgacg
      541 aggtggaaat cgtcgaagac gagggctgtc gggtcgttcg tcagcccgcc gagctcgcgg
      601 aggaacgcat cgaggacggt ctcgagcggc ggcgcggcgg tcgcggtctc gcggagcaag
      661 gcgctcgtcg cctggccgaa gtcgggcacg tggatccgaa ccgcggccac gagatacgcg
      721 aggaagccga cccagtcgcg gtcgccccgg tcgagtcgaa accagagaac ccgaatgcgc
      781 gtgcgccggg tgaagtcggc gaggagggtc gtcttgccgt agcccgcctc ggcgatcagg
      841 aggacagcgc ggcggtggat cttgaccgac agccactcga gcagccggtc gcgcgccagc
      901 gtctcctcgc ggagcggcgg cgcctggacc ttgctgatct ggaccgggta ctcggacaaa
      961 ccgaggtgcc ctccggtgga cgcgatgctg ccgacgatgg cgggacgccc cggctcgacg
     1021 atgagtcgcg gtcgccgtcg ccgagatcgc ggatcggagg cccggtcggg cttgggcggc
     1081 tcgacatcgg gatcgacgcg cggctcacgc tcgatggccc gatcgagcgg cgcgaacgat
     1141 agggatgggt cgcgcggcgt gtcaacgacc gccgacgact tgcgggcggc cgacggtcgg
     1201 ctgggcatga ccggaatctt ggccgagccc gatcaccgct cgattacggc ctcgtggatt
     1261 gtctgtggac aagccgggtc tggcccggcg cgctcatcga tgcctgagcg tcagccctcg
     1321 tattcgaagc cgatcttgat gtcgactcgg tactcgacga tctcgccgtc ctcgacgttg
     1381 gcgctcgagt gcacgacctc gacggtcttg atgttgcgca ccgtgcgcga ggcggtcgcc
     1441 acggcctggc gggccgcgtc gctccatgac ttcgggctcg tcccgaccat ctcccgaacg
     1501 atgatcacgc ccatctcgat gtcctccggc tggcctgtcc ctggacggcc agcatagtca
     1561 ggaggagacc ggggccgccg cgggtgagat cacgccggcg cggaccttgt cgaagcaggt
     1621 gctgcagtag accggtcggt cggtgcgcgg cgcgaacggc accatcgcct ggtcgccaca
     1681 ggcgttgcac acggcggcgt ggtactcgcg cggcccaccc attcgcaccc gtttcgcgtt
     1741 cgcacggcag cccgggcagc gttgggggac gttctggagg cccttctcgg cgaagaaccg
     1801 ctgctccccg cccgagaaga tgaacccatc gccgcagtcg cgacaggtga gcgtccggtc
     1861 ttcgtacacc gtgatcgacc tccggtgagg gtccgtacga tcccgttccg ctccccactt
     1921 cggaacgctg gcggcgagaa tacggctcgc gtcaagccgg cccgcagcga tctcgcgagc
     1981 gttcgtgctg cgcggtgcgc gggggcaccg gcaacgagcg ctgcgagccg agttgggaac
     2041 catccggcgc tcgccgccgt cccgccgcca tgaccgacac attgaccctt cgttccgcgc
     2101 gcccggtggc cgtcgtcgcc cgcgccgccg cggcgcccat ccttctcgcg gccctgatcg
     2161 ccgcgtgctc gaccgccgca gctccatcgc cgacaccagg cgccacgggc agtccgtcag
     2221 ccccaccaac cgccgcccca acctcgaccc cagagatcga cggcatccag catccgaccg
     2281 gcgccaccga cgtggtcctg cgcatggaag agggtggcgg cttcgtccca atcgacttcc
     2341 tggccacttc cgccccgcag ttcaccctct acggcgacgg gaccgtcgtc ttccgcgacc
     2401 cgaccgcgac gctgcccgag cccgtcggcg gggtgactcg ctccgcaccg ttcctgatcg
     2461 tgaacatcgg tgagggaggc atccaggcgc tcctgaccga ggcgatcgga cccggaggtc
     2521 tcggggccgc cgtcggtccg tacatgggcc agggcgccga catgtcgacg acgaccttca
     2581 cgatcaatgc cgacggcgat accaagcagg tcagcgtttc cggcttcctg cccgacctgc
     2641 acccgcagga cgcggtcatc gtcggcgcgc tcggccggct cgccgagcga ctgtcgggct
     2701 tcgccgacgc gatcgcgggc gaggccaagt acgtccccgc cgcctaccgc ggcatcctga
     2761 tgccggtgga ccagccgttc ggaccggtca tcgcctggcc ctggacggac atcacacccg
     2821 ccgagttcgt cccgggcgac aacgagttct tcatgacccg cacgatgacc ctcgccgagg
     2881 tcgcggtgct cgagatcccg ggcgccgagg gcggcttcct gggcctcacg ctcaagcacg
     2941 agggcaagct gttcagcttc tcgctccgac ctctcctccc cgacgagacc aagtagccga
     3001 accttggggc gccttggatc aggcgcccga tcagacgccg gcgccctcgt aacgctggcc
     3061 ggcgtacgac tggaatcgag tctggctctt gcggaaccag agctggatgt cgcccgtggg
     3121 gccgttgcgg tgcttcgcga gcttgaggtt gatgacctcg ccgtcgaggt cggcctcctc
     3181 gccgaccttt tccttctcgc ggtagaggaa gagcacgagg tcggcgtcct gctcgatcgc
     3241 gccagactcg cggaggtcag aaagcctcgg ctccttcgac tcgcgcatct ccggctggcg
     3301 cgagagctgg gagagggcca tgaccgggac ggaaagctcg cgagcgaggg ccttgagccc
     3361 gcgcgagatc tcgctgacct cctggacgcg gttcgagtcg cggttctgcg tcgtcgcctg
     3421 catcagctgc aggtagtcga cgatgaccag atcgaggccg gactcggcct ggagccggcg
     3481 cgccttcgtg cgcagctcca tcgaagtgat gttcggcgtg tcgtcgatgt acagcgcggc
     3541 ctcggaaagg tcgttcatcg ccggtgcgat ccgggcgaag tccagctcct cgaggaagcc
     3601 ggagcggagt cgcttcgagt caatgtttgc gacgctcgag agcagacgaa gcacgagctg
     3661 ctccttgctc atctcgaggc tgaacagcgc gacggtcttg cgctccttca ctgcggcgtg
     3721 ctcggcgacg ttgagcgcga agcttgtctt gccgaccgaa ggccgggccg cgaggacgac
     3781 gaggtcgctc ttctggaagc ccgtggtcag ctggtcgagg tcgaggaagc ccgacgagat
     3841 gccactgagc tcgccgcggt gggcgtggag gtagtcgagc cggtcgtagg cggagtggag
     3901 aagatccttc aaatggctga agccggtcgt gatcc
//
LOCUS       DATBSW010000102        11044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_995438, whole genome shotgun sequence.
ACCESSION   DATBSW010000102 DATBSW010000000
VERSION     DATBSW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995438"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VIF63_02890"
     CDS             <1..657
                     /locus_tag="VIF63_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8238357.1"
                     /translation="DRRGRGASGDSDAYAIEREFEDLAAVAEALAAATHQAAIDVCGH
                     SYGGRCGLGASLLTPAIRRVVVYEGAPAPPGVSYRPPGLVEAVRGALERGDKEAALTT
                     FLAGIVGMSDAALDAYRADPVWPARVTAAHTILREIEAEAGPAASIEALGRATAPVLL
                     ILGSISRSPFRLGTDALAARLADALVVTIEGAAHAAHHTHVPEFVAAVTAFLDAASHR
                     "
     gene            694..1287
                     /locus_tag="VIF63_02895"
     CDS             694..1287
                     /locus_tag="VIF63_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238358.1"
                     /translation="MTDWDTGIPDPAQPDGTATPATSPTCPWCNASVKPEQTNCPACG
                     AALAQRESIAGVKIPGLTSVDPALEDFDKRPLHLRGPSPSQGVAPGLIIAAAAGGPIG
                     MLAIGGVAAVAAAELMGTKRDGPAGTSFENVGQPSELLLQAIEHADAGGDGQADAPEA
                     TAVEAEPDHAADDGGRSVWRDLPPSDDASDSRDEDPR"
     gene            1324..2418
                     /locus_tag="VIF63_02900"
     CDS             1324..2418
                     /locus_tag="VIF63_02900"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY8238359.1"
                     /translation="MTDERPYSPGLEGVVAAETALGYVDGERGRLLYRGYRIGDLVEH
                     GTYPAIANLLWTGEWAPDHHLPTTPVPDAVMVALRALPADAKPMDALRTAVSVWGATQ
                     VLDWPPTPEQARALTAFSPSALAAFARLRKGQEPIEPDPSLDLVPGFLYQLNGERADP
                     ATARALDAYFIVGAEHGLNASTFTARVITSTRSDLASAVVGAIGALKGPLHGGAPSEV
                     VEQIHNAGSPEHAEQWVRETIAKGERLMGFGHRVYRAYDPRAAALRKVAESMEHKPEW
                     LDLAIAIEDVALRVLAELHPERALKTNVEYYAAPVLMGVGLTPDLFPATFALARHAGW
                     TAHVIEQAGANRIIRPDARYTGPGERDLPA"
     gene            2524..3378
                     /locus_tag="VIF63_02905"
     CDS             2524..3378
                     /locus_tag="VIF63_02905"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8238360.1"
                     /translation="MDAQGVWTKPLGDLLHRFFFWFFGLMPPVRDLLVGRWLGHPIHA
                     VLTDVPIGILFLVIVFDVVAMPDAAFWALVFGILAMLGAAVAGYADYADTDGKARERA
                     TLHSTLMLLALVFYLVSLWLRSGKGPQDAVDGPAALLSIAGFLILSAGAYVGGDVVYV
                     LGNMVNRHAFRGAGTKWIALEPAEVDADGAIPEGKPVKAKLGINQLVLVRQGEKILAL
                     HDTCAHAGGPLSEGKVVDGTIECPWHYSRYKLDSGYVVRGPSVYDQPAYEVRARDGGG
                     WEARRKVG"
     gene            3396..3932
                     /locus_tag="VIF63_02910"
     CDS             3396..3932
                     /locus_tag="VIF63_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238361.1"
                     /translation="MPRPSVLRLPDPCLVVLIGAAGAGKSTLAARLFPPESILSSDAF
                     RAVVSGDAADQRATRTAFSILHRQLERRMAGRHTTVIDATSVTSYARRSLVRRAAAQG
                     IPVVAIVLDLDIDVVLAQNAGRSGRIVPEAVVRRHVADLARSLRPGALDREGFAVIHR
                     IRTPAGLDGLTIGWFGRG"
     gene            complement(3972..4841)
                     /locus_tag="VIF63_02915"
     CDS             complement(3972..4841)
                     /locus_tag="VIF63_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8238362.1"
                     /translation="MPDALTPPSLAVVALGLLASLGWGISDFGGGLASRRAPVLGVLL
                     WSQVASLALGVPLLLLTPEPPMQPQDFLIAALGGTLGASGLALLYRGLSIGRMGVVAP
                     VAAVLTAAMPVLFGIVTQGPPPVLAIVGIGCAVAGVVLVSRSPSAPHGRPSGLWYGIG
                     AGMFFGSFTLSAAFLGDGLIVTPVVIIRFASILTVAMWILLRRQSWRVSRGIIPALLV
                     IGVVDMSATASYLLAIAVGPLAIAAILASLYPVFTTVLAAAVLRERVTAMHAVGIVAA
                     LAAVVLIAGASAA"
     gene            4864..6525
                     /locus_tag="VIF63_02920"
     CDS             4864..6525
                     /locus_tag="VIF63_02920"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY8238363.1"
                     /translation="MRRVAAAFAALALAGLLVGPAIAADFVLPPSEEGRAVYDLAGIW
                     TSDTEAEAESIALAIRNRTLAQLAIVSWPSVDGDVSTETAREDALTIMNTWGVGREGV
                     DDGLVVLFDMDNGSRNHGQIYLYAGKGFIEQYMDPDEAAEIVNGKMLPPAIDGDIDGA
                     LLAGLHAVEHVAQEGGNPDRAGRNTFYAILVAIVLGMGLLVFGWFLRTWWERGRDAEV
                     PLIDDSVLLPVPPPGLTPALATVLRNDGVDKDSFTSALVDLGHRGLVTFQQPDGDKKH
                     VDLAIPPSPLIDPNSLEARRRPLGAAEGELADSIARKSVGGILSWTQLKAGEGAKLYA
                     SFKKDLGRSAKASGFFREDPNKLPARWTAIAIVLIVLVVAFGVVFAFDLNESSSELFK
                     PGRGFLLLPMLASIAMAILTMVFSGRLVARTRDGAQALGMALAYRNTLRYELKKAHTV
                     DEAVEQTKTRLPWITTPDLLTVWAVAFGLKDEIDDLIKETFETAQSTGAAVWAPIWYS
                     GSDGISSVGNLASSIGSISTSATSSSGSGYGGGGGGGGGGAGGGF"
     gene            complement(6522..8888)
                     /locus_tag="VIF63_02925"
     CDS             complement(6522..8888)
                     /locus_tag="VIF63_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238364.1"
                     /translation="MRITRLQLRNVRRHADLDLALAPGLTVVRGPNESGKSTIQRAIE
                     LALTRRVTSGSGDLDGMRSWNSSDEDRPWVRLEFEQEDLDGTRAGTLEKAFRGARGTV
                     KLELDTETITDPARADEVLADLTGIPSEPFFRSTASVRHHELDGLARDEAALRDRLQA
                     SISGADRGTSRARRKLEKALYDLNTKGDKNPGRIKAAEANLATATAAQRNGEAALTEL
                     EKDRDALAQAREERAVAEAELTEQRSLLEKARLAERLIGERDQARERFERYSTAVDVS
                     KQIDVLESSHPSSNGLPALREALSKVRAADLRIHEIRAALAGEVEVSFVVNEPTPRSW
                     RPTAIAAMVVIVLAVGVVAGDFLSLLPRELRTLSIGAGGSTITVPGAPVLAVLLVLFG
                     IGLALVGRRQRIRATEMGKTKDLRSQEIERRLRGRSMLEQELQMEEVTLTNLLSVLEQ
                     PDLAAVEALVTAEETHMSSIMRLRAQLEGLVGREPTETLPDLRDKAALDVEQKTGALE
                     QLGPIAKEPRARERLEVAVADAERGVELARDAESQARARVEQNAVDAEEVAGHAERAA
                     MWAEQLAALQRRARVYGATLGALDTAERATIRTATRYLEKRMVVDLERVTAGRYRRVK
                     VDDETLGLRVYAPERGDWVDVTTLSQGTLDTVYLAARISLVRLVTGDRRPPLVLDDPF
                     VTLDDARATRALDLLHDISADFQVIYLTTSDRYDSIADKVCQLEPATALTPDLDPNEI
                     EVEPEPAPAAPATPEAEPVEASTNGASAPASAEVAPVEPTPPEAAPVG"
     gene            complement(8885..10276)
                     /locus_tag="VIF63_02930"
     CDS             complement(8885..10276)
                     /locus_tag="VIF63_02930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEY8238365.1"
                     /translation="MPPKDTPDLRMRPSADRSSCQRTCHNDRRPFVQASGEQLGAPRS
                     PGHQPGTGAHGEIATGMLRLLHTADVHLGARHADLGDRAAMQRERQFAAFTATIDLAL
                     EEKVDLVLIAGDLFDSNVQPRRSVERAAAQLKRLVEARIRTVIAPGTHDVYDRASIYR
                     AYDLAAMSGAVGSDLVTVLDPEHVEVHLKPLDLIVHGRCFATKRAPTSPLLDLDVAKD
                     DRAAWHVGLLHAALAIEGRTDGDDVVITSAEIAASHLDYLALGHWHSTTRGKAGKTAY
                     AYSGAPEPVALDQDRAGNVLLVQLDAHDGKKQVSVDERKVGKTHFERIQLDAAKVGSQ
                     PGLVSKLAEKADPDLVLDVELIGVRPDELDVHVEEVEAELAERFLKVRVRDRSVAPLP
                     EGMIASPDTVLGAFIRDLEARIAEVEAADGTAPAGTDLGDGGVAAADTEAMELRDALR
                     LGRLLLAGTEVTL"
     gene            10413..10745
                     /locus_tag="VIF63_02935"
     CDS             10413..10745
                     /locus_tag="VIF63_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY8238366.1"
                     /translation="MTYADRTLACVDCGSEFIHSADDQKYYTEKGFSSDPKRCVSCRA
                     SRRAARDTGYTATDLGGPRGYERGGTTREYFAVLCSECGNQAQVPFRPRMDRPVYCSD
                     CFRKANPD"
BASE COUNT         1571 a         3968 c         3878 g         1627 t
ORIGIN      
        1 gaccggcgcg gtcgcggcgc atccggcgac agcgacgcgt acgcgatcga gcgggagttc
       61 gaggacctcg ccgccgtggc ggaggcgctc gccgccgcca cgcaccaggc cgccatcgac
      121 gtctgtggcc actcctacgg cgggcgctgc gggctcgggg ccagcctcct gacgcctgcg
      181 atccgccgcg tcgtcgtgta cgaaggcgcg ccggctccgc cgggcgtgtc gtatcggccg
      241 ccgggcctgg tcgaagcggt gcgcggcgcc ctcgaacgcg gcgacaagga ggcagcgctg
      301 accacgttcc tcgcgggcat cgtcggcatg tccgacgccg ccctcgatgc gtatcgcgcc
      361 gatccggtat ggccggcgcg cgtgaccgct gcgcacacga tcctccggga gatcgaggcc
      421 gaggccgggc ctgccgcctc gatcgaagcg ctcggccgcg cgacggcgcc ggtcctgctc
      481 atcctcggct cgatcagccg ctcgccgttc cgcctcggca cggacgccct cgccgcccgt
      541 ctcgccgacg cgctggtcgt gacgatcgag ggcgcggccc acgcggcgca tcacacccac
      601 gtcccggagt tcgtggccgc ggtcaccgcg tttctcgatg cggcgtcgca ccgctgaggg
      661 cgaatcgccg gaatcaggcg gtatcctccg cgcatgaccg actgggacac gggcataccg
      721 gatccggcgc agcccgacgg gaccgcgacg ccggcaacat cgccgacctg tccgtggtgc
      781 aacgcatcgg tcaaacccga gcagaccaac tgcccggcgt gcggcgcggc gctggcccag
      841 cgcgagtcga tcgccggcgt gaagatcccg ggcctgacct cggtcgatcc agcgctcgag
      901 gacttcgaca agcgcccgct ccacctccgc ggcccgagcc cgagccaggg cgtcgcgccg
      961 ggcctgatca ttgcggcggc cgcgggcggc ccgatcggca tgctcgcgat cggcggcgtc
     1021 gccgccgtgg cggcggccga gctgatgggc acgaagcgcg acggcccggc aggcacctcg
     1081 ttcgaaaacg tcgggcaacc gagcgagctg ctcctccagg cgatcgagca cgccgacgcc
     1141 ggcggcgacg gccaagcgga tgcgcccgaa gcgaccgcag tggaggcgga gcccgaccac
     1201 gcggccgacg acggcggccg ctcggtctgg cgtgaccttc cgccatcgga cgacgcgtcg
     1261 gattcgcgag acgaggaccc gcgctagcgg ccccgaatcg gacccgcggc taccatcgag
     1321 ccgatgactg acgagcgacc ctactcaccc ggcctcgaag gggtcgtcgc tgccgagacc
     1381 gcgctcggct acgtcgatgg cgagcgtggc cgcctcctgt accgcggcta tcgcatcggc
     1441 gacctcgtcg agcacggtac ctacccggcc atcgcaaacc tcctctggac aggcgagtgg
     1501 gcgccggacc atcacctgcc gacgacaccg gtgcccgacg ccgtgatggt cgcgctgcgg
     1561 gcgctgccgg ccgacgccaa gcccatggat gcgctgcgca ccgcggtgtc cgtctggggc
     1621 gccacgcagg tgctcgactg gccgccgacc cccgagcagg cgcgcgcgct cacggccttt
     1681 tcgccgtcgg cgctggccgc gttcgcacgg ttgcgcaagg gccaggagcc gatcgagccc
     1741 gacccgagcc tcgacctcgt acccggcttc ctgtaccagc tcaacggcga gcgcgcggac
     1801 cctgccaccg cgcgcgccct cgacgcgtac ttcatcgtcg gcgccgagca cgggctcaat
     1861 gcctcgacgt tcaccgcccg cgtcatcacc tcgacccgca gcgacctcgc ctcggcagtc
     1921 gtcggcgcca tcggcgcgct gaaggggccg ctccacggtg gcgcgccgtc cgaggtcgtc
     1981 gagcagatcc acaacgccgg ctcgcccgag cacgccgagc aatgggttcg cgagacgatc
     2041 gcgaagggcg agcgcctgat gggcttcggg catcgggtct accgcgcgta cgacccgcgc
     2101 gccgcggcgc tgcgcaaggt cgccgagtcg atggagcaca aaccggagtg gctcgacctc
     2161 gcgatcgcca tcgaggacgt tgccttgcgc gtcctcgccg agttgcaccc cgagcgcgcg
     2221 ctcaagacga acgtcgagta ctacgcggcg cccgtgctca tgggcgtcgg cctcaccccg
     2281 gacctcttcc ccgccacgtt cgcgctggcc cgccacgccg gctggactgc gcacgtgatc
     2341 gagcaggcgg gcgcgaaccg gatcatccgg ccggatgctc gctataccgg ccccggggag
     2401 cgcgacctcc ccgcctgacc ggcccgtgaa attcgatccg gaccgcgacg ccggtcgtag
     2461 actcagccga ctgctccgcg cccaggaggt tcggtcgtgc tgcctcgttg gctgactcga
     2521 gtgatggacg cccagggcgt ctggaccaag ccgctcggcg acctgctcca tcgcttcttc
     2581 ttctggttct tcgggctgat gccgccggtg cgcgacctgc tcgtcgggcg ctggctcggc
     2641 cacccgatcc acgccgtcct gaccgacgtc ccgatcggga tcctgttcct cgtcatcgtg
     2701 ttcgacgtcg tcgcgatgcc cgacgccgca ttctgggcac tggtgttcgg catcctggcg
     2761 atgctcggtg ctgccgtcgc cggctacgcc gactacgcgg acaccgatgg caaggcgcgc
     2821 gagcgagcga cgctccattc gacgctcatg cttctcgcgc tcgtcttcta cctcgtctca
     2881 ctgtggctcc ggtccggcaa gggcccgcag gacgcggtcg atgggcccgc ggcgttgctc
     2941 tcgatcgccg gcttcctgat cctgtccgcc ggcgcctatg tcggtggcga tgtcgtgtac
     3001 gtgctcggca acatggtcaa ccggcacgcg ttccgcggcg ccggcacgaa gtggatcgcg
     3061 ttggagccgg ccgaagtcga cgcggacggc gccatccccg agggcaagcc cgtcaaggcg
     3121 aagctcggga tcaatcagct cgtcctggtc cgccagggcg agaagatcct cgcgctgcat
     3181 gacacatgcg cccacgcagg cggaccgctc agcgagggca aggtcgtcga cggcacgatc
     3241 gagtgcccgt ggcactactc ccgctacaag ctcgacagcg ggtacgtcgt ccgcggcccg
     3301 tcggtatacg accagcccgc gtacgaggtt cgggcgcgcg atggcggcgg ctgggaggca
     3361 cgtcgcaagg tcggctgatc cggcacgatg gggccgtgcc acggccctcg gtgctccggc
     3421 tgcccgatcc ttgtctcgtc gtcctgatcg gcgcggcggg cgccggcaag tccaccctcg
     3481 cggcgcggct gttcccgccc gagtcgatcc tgtcatcgga cgcgttccgc gccgtggtct
     3541 cgggcgacgc cgctgaccag cgggcgaccc ggacggcgtt ctcgatcctc catcgacagc
     3601 tcgagcggcg gatggccgga cggcacacga cggtgatcga tgccacgagc gtcacgtcgt
     3661 acgcgcggcg ctcgctcgtc cggcgggcgg cagcacaggg cattccggtc gtggcgatcg
     3721 tcctcgacct cgacatcgat gtggtgctgg cccagaacgc cggacggagc gggcggatcg
     3781 tgcccgaggc ggtcgttcgc cggcacgtcg cagacctcgc gcggtcgctt cggcccggcg
     3841 cgctcgatcg cgaggggttc gcggtgatcc accggatccg gactcccgcc gggctggacg
     3901 gactcacgat cgggtggttc gggcgtggct gacgcgccgg gctgacgcgc ggcctcgact
     3961 cgaggagggg ctcaggcggc ggacgcaccc gcgatcagga ccaccgcggc aagggctgcg
     4021 acgatgccca ccgcatgcat cgcggtgact cgttcgcgca ggacggcggc ggcgaggacc
     4081 gtcgtgaata ccgggtagag cgaggccagg atcgccgcga tcgccagcgg cccgaccgcg
     4141 atggcgagca ggtaggaggc agtcgccgac atgtcgacca cgccgatcac gagcagtgcc
     4201 gggatgatgc cgcgcgagac ccgccacgac tggcggcgca agaggatcca catcgccacg
     4261 gtcaggatcg acgcgaagcg aatgatcacg accggcgtga cgatgaggcc gtcaccaagg
     4321 aacgccgcgg agagcgtgaa cgagccgaag aacatgccgg cgccgattcc gtaccagaga
     4381 ccgctgggcc gcccatgcgg cgccgaaggg ctgcgcgaca cgagcacgac gccggccacg
     4441 gcgcacccga tgccgacgat cgccaggacc ggcggcggac cctgcgtgac gatgccgaac
     4501 aggaccggca tcgcggcggt gagcacagcc gccacaggcg cgacgacgcc catccgtccg
     4561 atgctcaggc cgcgatacaa cagggccagg cccgacgcgc cgagcgtccc gccgagcgcg
     4621 gcgatcagga aatcctgtgg ctgcatcggt ggctccgggg tcagcagcag gagcggcacc
     4681 ccgagcgcga ggctggcgac ctggctccac agcagcacgc cgaggacggg cgcgcggcgg
     4741 ctggcgagcc cgccgccgaa atcggagatg ccccagccca gggacgcgag caggccgagg
     4801 gcgaccacgg cgagcgatgg cggggtgagg gcgtcgggca tccgcatatc atcgcgactg
     4861 cccatgcgtc gcgtcgccgc tgcctttgcc gctctcgccc tcgccggctt gctggtgggt
     4921 cccgccattg cggcggactt cgtcctgccc ccgagcgagg agggccgcgc cgtctacgac
     4981 ctcgcgggca tctggacgtc cgacaccgag gcagaagccg agtcgatcgc cctcgcgatc
     5041 aggaaccgga ccctggccca gctcgcgatc gtctcgtggc cgagcgtgga cggcgacgtc
     5101 tcgaccgaga ccgcccgcga ggacgcgctc acgatcatga acacgtgggg cgtcggccgg
     5161 gagggcgtcg atgacgggct cgtcgtcctc ttcgacatgg acaacggcag ccggaaccac
     5221 ggccagatct acctctatgc cggcaagggc ttcatcgagc agtacatgga tccggacgag
     5281 gcagccgaga tcgtcaacgg caagatgctg ccgccggcga tcgacggcga catcgacggt
     5341 gcgctcctcg ccggcctgca cgccgtcgaa cacgtcgcgc aggagggcgg caacccggac
     5401 cgcgccggca ggaacacgtt ctacgcgatc ctcgtcgcga tcgtgctggg catgggcctg
     5461 cttgtcttcg gctggttcct gcgcacgtgg tgggagcgcg gccgcgacgc cgaggtgccg
     5521 ctgatcgacg attcggtgct cctgccggtc ccgccgcccg ggctgacccc ggccctcgcc
     5581 accgtgctcc gcaacgacgg tgtcgacaag gacagcttca cctcggcgct ggtcgacctg
     5641 ggacatcgtg ggctcgtgac gttccagcag cccgacggcg acaagaagca cgtcgacctc
     5701 gcgatcccgc cgtccccgct catcgacccg aactcactcg aggcgcggcg ccggccgctc
     5761 ggcgccgcgg agggggagct tgccgattcg atcgcgcgca agtccgtcgg cggcatcctg
     5821 agctggacgc agctgaaagc cggcgagggc gcgaagctct acgcctcctt caagaaggat
     5881 ctcggccggt cggccaaagc gtcgggcttc ttccgcgagg acccgaacaa gctgcccgcc
     5941 cgttggaccg cgatcgcgat cgtcctgatc gtgctcgtgg tggcgttcgg cgtcgtcttt
     6001 gccttcgatc tcaacgagag ctcgagcgag ctgttcaagc cggggcgcgg cttcctgctc
     6061 ctgccgatgc tcgcctcgat cgccatggcc atcctgacga tggtcttcag cggccggctc
     6121 gtggcccgca cgcgcgacgg cgcgcaggcg ctgggcatgg ccctcgccta caggaacacg
     6181 ctgcgctacg agctcaagaa ggcgcacacg gttgacgagg ccgtcgagca gaccaagacc
     6241 cggctgccct ggatcacgac gccggacctg ctcacggtgt gggcagtcgc attcgggctc
     6301 aaggacgaga tcgacgacct catcaaggag acgttcgaga cggcgcagtc caccggagcg
     6361 gccgtgtggg cgcccatctg gtactccggc agcgacggga tcagctccgt cggcaacctg
     6421 gcgagctcga tcggctcgat ctcgacctcg gccacgtcat cgtcgggctc cggctacggc
     6481 ggcggcggtg gcgggggtgg cgggggcgcc gggggcggct tctagccgac cggcgcggcc
     6541 tcgggcggcg tcggttcgac aggcgcgacc tcggccgagg ccggcgcgga cgcgccattc
     6601 gtcgaggcct cgactggttc cgcttcgggc gtcgctggcg ccgcgggcgc gggttcgggc
     6661 tcgacctcga tctcgttggg gtcgaggtcg ggggtgagcg cggtggccgg ctcgagctgg
     6721 cagaccttgt cggcgatcga gtcgtatcga tcggaggtcg tcaggtagat gacctggaag
     6781 tccgcgctga tgtcgtggag caggtcgagg gcgcgcgtcg cacgcgcgtc gtcgagggtc
     6841 acgaacgggt cgtcgagcac gaggggcggc cgccggtcgc cggtcacgag gcgcacgagg
     6901 ctgatccggg cggcaaggta gacggtgtcg agcgtgccct ggctgagggt cgtgacgtcg
     6961 acccagtcgc ctcgctcggg ggcgtagaca cggaggccga gcgtctcgtc gtcgaccttc
     7021 acccggcggt accggccggc cgtcacgcgc tcgaggtcca ccaccatgcg cttctcgagg
     7081 tagcgggttg ccgttcgaat cgtcgcccgc tccgcggtat cgagcgcgcc gagggtggcc
     7141 ccgtacacgc gagcgcggcg ctggagcgcg gccagctgtt ctgcccacat cgcggcgcgc
     7201 tcagcgtgcc cggcaacttc ttctgcgtcg acggcgttct gctccacccg ggcccgcgcc
     7261 tgtgattcgg cgtcgcgcgc gagctcgacg ccgcgctccg cgtcagcgac ggcgacctcg
     7321 agtcgctccc gagcgcgcgg ctccttcgca atcggcccga gctgttcgag cgcgccggtc
     7381 ttctgctcga catcgagcgc cgccttgtcg cggaggtcgg gcagcgtctc ggtcggttcg
     7441 cgcccgacga ggccttcgag ctgggcacgg agccgcatga tgctgctcat gtgcgtctcc
     7501 tcggccgtca cgagcgcctc cacggcggcg agatccggct gttccagcac ggagaggagg
     7561 ttcgtcagcg tgacctcctc catctggagc tcctgctcga gcatcgaacg gccgcgcagt
     7621 cgccgttcga tctcctggct gcgcagatcc ttggtcttgc ccatctccgt cgcgcggatg
     7681 cgctggcgac gaccgacgag cgcgagcccg atcccgaaca ggaccagcag cacggcgagg
     7741 accggcgcac cgggcaccgt gatcgtgctg ccacccgccc cgatgctgag cgtcctcagc
     7801 tcacggggca gcaggctcag gaagtcgccg gcgaccacgc cgacggcaag cacgatcacc
     7861 accatcgccg cgatcgcggt cggacgccac gagcgcggcg tcggctcgtt gacgacgaag
     7921 gagacctcga cctcgccggc gagggcagcc cggatctcgt ggatccggag gtccgccgca
     7981 cggaccttcg agagggcctc gcgcagggcg ggcaggccgt tcgaggacgg gtggctcgac
     8041 tcgagcacgt cgatctgctt gctcacgtcg accgcggtgc tgtagcgctc gaaccgctcg
     8101 cgcgcctggt cgcgttctcc gatgaggcgc tcggcgaggc gggccttctc gaggagcgag
     8161 cgctgctcgg tcagctccgc ctcggcgacg gcgcgctcct cgcgtgcctg ggccagcgca
     8221 tcgcggtcct tctcgagctc ggtgagcgcc gcctcgccgt tgcgctgggc ggccgtcgcg
     8281 gtcgcgagat tcgcctctgc cgccttgatc ctgccggggt tcttgtcgcc cttggtgttg
     8341 aggtcataga gcgccttctc gagcttgcgc cgcgcccggc tcgtgccccg atcggcgccc
     8401 gagatcgagg cttgcaggcg gtcgcgcagg gcggcctcgt cgcgggcaag gccgtccagc
     8461 tcgtgatgcc tgacggacgc ggtcgatcgg aagaagggct cgctcgggat cccggtcaga
     8521 tcggcgagca cctcgtcggc gcgggccgga tcggtgatcg tctctgtatc gagctcgagc
     8581 ttgaccgtgc cacgcgcgcc gcggaacgcc ttctcgagcg tgcccgcccg cgtcccgtcg
     8641 aggtcctcct gctcgaactc gagccggacc cacggccgat cctcgtcgct gctgttccac
     8701 gagcgcatgc cgtcgaggtc ccccgagccc gacgtcacgc gacgtgtcag cgcgagctcg
     8761 atcgcccgct ggatcgtgga cttgcccgac tcattcgggc cgcgaacgac cgtcaggccc
     8821 ggtgcgagcg cgaggtcaag atcggcgtgg cgccggacgt tgcggagctg gagccgggtg
     8881 atcctcacag ggtgacctcg gtcccggcga gcagcagccg gccgaggcgg agcgcgtcgc
     8941 gcagctccat cgcctctgtg tcggccgcgg ccacgccgcc gtcgcccaga tccgtgccgg
     9001 ccggggcggt gccgtcggcc gcctcgacct cggcgatccg ggcctcgaga tcacggatga
     9061 acgcaccgag caccgtgtcg ggcgaggcga tcatgccctc gggcagcggc gcgaccgatc
     9121 gatcacggac gcgcaccttc aggaaccgct cggcgagctc cgcctcgacc tcctcgacgt
     9181 ggacgtcgag ctcgtccggt cgcacgccga tcagctcgac gtcgaggacg agatccgggt
     9241 ccgccttctc cgccagcttc gacaccagcc cgggctgcga gccgaccttg gccgcatcga
     9301 gctggattcg ctcgaagtgc gtcttgccga ccttgcgctc atcgaccgag acctgcttct
     9361 tgccgtcatg cgcatcgagc tggaccagga gcacgttccc ggcgcggtcc tggtcgagcg
     9421 cgaccggctc gggcgcgccg gagtaggcgt atgccgtctt gccggccttg cccctcgtcg
     9481 tcgagtgcca gtgcccgagc gcgaggtagt cgaggtggct cgcggcgatc tccgccgacg
     9541 tgatcacgac gtcgtcgccg tcggtcctgc cctcgatcgc gagcgcggcg tggagcagcc
     9601 cgacgtgcca cgcggcgcgg tcgtccttcg cgacgtcgag gtccagcagc ggcgacgtgg
     9661 gcgcgcgctt cgtggcgaag catcgaccgt ggacgatgag gtcgagcggc ttgaggtgaa
     9721 cctcgacgtg ctcgggatcg agcacggtca caaggtccga gcccaccgca cccgacatcg
     9781 ccgcgaggtc gtaggcgcgg tagatcgacg ctcggtcgta gacgtcgtgg gtgcccggcg
     9841 cgatcaccgt gcggatgcgc gcttcgacga ggcgcttgag ctgcgccgcc gctcgctcga
     9901 cggagcgccg cggctggacg ttcgagtcga acaggtcgcc cgcgatcagg acgaggtcga
     9961 ccttctcctc gagcgcgagg tcgatcgtgg ccgtgaacgc tgcgaactgc cgctctcgct
    10021 gcatggcggc gcgatccccg agatccgcgt gacgagctcc gagatgcacg tccgccgtgt
    10081 ggagcagtcg cagcatgcct gtggcgatct ccccgtgcgc cccggtgccc ggctgatggc
    10141 ctggcgagcg gggcgccccc aattgctccc cgctcgcctg cacgaacggt cgccgatcat
    10201 tgtgacaggt acgttggcat gaacttcgat cggctgacgg acgcattcgg agatccgggg
    10261 tatccttcgg cggcattcac ccggtgcgcc ccgaccaggg actccagacg gcaccaaggg
    10321 tcgcgtggca ccggctccgg ccgcttccgc gttgccgacg ggccccatct ccggacgcac
    10381 caccggcctt tcaacgcgct ccggaggctc ccgtgaccta cgccgatcga accctcgcct
    10441 gcgtcgactg tggctctgag ttcatccatt ccgcggacga tcagaagtac tacaccgaga
    10501 aggggttctc ctcggacccg aagcgctgcg tgagctgccg tgccagccgc cgcgccgccc
    10561 gtgacaccgg ctacacggcg acggacctcg gcggcccgcg cggctacgag cgcggtggca
    10621 cgacccgcga gtacttcgcg gtcctgtgct ccgagtgcgg caaccaggcg caggtcccgt
    10681 tccgcccgcg catggaccgc ccggtctact gctccgactg cttccgcaag gcgaatcccg
    10741 actgatcgca cgcccggcgc gcagctctgc ggatcggtga cgcgcgccca acgttcttgc
    10801 caaatcgaca cctggtgacg cttaggcgaa gtcgtgccgg ggtgggcgca ctccgtggca
    10861 ccgctcgcca cgagattcgc actccgcagg acgtcctcga acgaagttcg cagtccacgc
    10921 tcgggtcggg cgtccgtggt cctgcataag cgtcgtgtgg taacgatctg acaagaagcg
    10981 gcggcggatt tggaaacgga gagctgttcg acgagcgccc gataaactcg cgacgatgac
    11041 gacg
//
LOCUS       DATBSW010000103         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_78733, whole genome shotgun sequence.
ACCESSION   DATBSW010000103 DATBSW010000000
VERSION     DATBSW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78733"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VIF63_02940"
     CDS             complement(<1..327)
                     /locus_tag="VIF63_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY8238367.1"
                     /translation="MDELGEQLTGWGTVLKLETRGRVSGRSIDVAIGYVEEPDGSMLV
                     AATSAEADWARNLDVDPRCRVTVGDRSWPAEAEPISGAEAHRAVRELILRYGTPAERL
                     GSGPVFR"
     gene            complement(348..1535)
                     /locus_tag="VIF63_02945"
     CDS             complement(348..1535)
                     /locus_tag="VIF63_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY8238368.1"
                     /translation="MIEQEASPAQQRALPAFDSKVDRLRPTYGFEDVSLAPGIETIEP
                     NDVELGQSFCGIDLAIPVLAAAMDAVVDPGFAGALARLGGLAVLNLEGVQTRYEQPAE
                     VLDRIAAASDDEVQDVLSEAYAAPVRDDLVAKRIEELHAAGSKAAVAATPGAARRWGP
                     FCAEHGADLFLVQSQVSSARHLATGYDPLSLAEFTRFMPIPVAVGNTTNAEAAYLLME
                     QGAAAVFVGVGPGAACTTREVLGIGVPQVTAIADVAAARDAYHRETGRYVPVVADGGM
                     RRGGELAKAIACGADALMLGSPLARATEAPGHGVNWGMAAPSPVLPRGTRIKVGTVGS
                     LEKILFGPAGVSDGSQNLMGALRQSMAALGARTLHDLQQVEVVYAPSVQTEGKSWQTR
                     RPG"
     gene            complement(1563..2042)
                     /locus_tag="VIF63_02950"
     CDS             complement(1563..2042)
                     /locus_tag="VIF63_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8238369.1"
                     /translation="MSPLRPDLVACWLYRVTGDGQLEILLIHRAPGRMYPGLWQCVTG
                     RLEAGETIVAGALREVAEETGMGPAAIEALFETDIVNWFHEVAVDGLMSEAVFAARLR
                     LDAAITISDEHDDLRWLTPAEAHELVTWPSYHRAIEQLEWLVANPDRAAVYRLPDPT"
     gene            2097..2408
                     /locus_tag="VIF63_02955"
     CDS             2097..2408
                     /locus_tag="VIF63_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY8238370.1"
                     /translation="MTWHRAAPLDELRAARPWLPLTIEGVDLVVAVVDGVPYAVEDRC
                     AHAGCAFSEDADLVGDDIVCNCHGSEFDLRTGALKRGPAERPVRSIPVRIEGEHLEVA
                     L"
BASE COUNT          333 a          879 c          866 g          338 t
ORIGIN      
        1 tcggaagacc ggtcccgaac cgagccgctc ggccggcgtc ccgtagcgca ggatcagctc
       61 gcggacggcg cgatgggctt cggcgccgga gatcggctcg gcctccgcgg gccatgaccg
      121 gtcgccgacc gtgacccgac atcggggatc gacgtcgagg ttgcgcgccc agtcggcctc
      181 ggccgacgtt gcggcgacga gcatcgagcc atcgggctcc tcgacatagc cgatggccac
      241 gtcgatcgat cggccggaca cgcggccgcg cgtctcgagc ttgagaaccg tcccccagcc
      301 ggtgagctgt tcgccaagct cgtccatcgg cccggctcag ccgcggatca gccgggccgt
      361 cgcgtttgcc aggacttgcc ctcggtctgg accgagggcg cgtagaccac ctcgacctgc
      421 tggaggtcgt ggagcgtcct cgcgccgagg gcggccatcg actggcgcag ggcgcccatc
      481 aggttctgcg agccgtcgct caccccggcc ggcccgaaga ggatcttctc gagggagccg
      541 accgtgccga ccttgatccg cgtcccgcgc ggcagcaccg gcgacggcgc ggccatgccc
      601 cagttcacgc cgtggcccgg cgcctcggtc gcgcgcgcca gcggcgagcc cagcatcagc
      661 gcatcggcac cgcacgcgat ggccttcgcc agctcgccac cgcggcgcat cccgccgtcg
      721 gcaacgaccg gcacgtagcg gccggtctcg cggtggtagg cgtcgcgtgc cgccgcaacg
      781 tccgcgatcg cggtcacctg gggcacgccg attccgagca cctcgcgcgt ggtgcacgcg
      841 gcacccggcc cgacgcccac gaagaccgcc gcggcgccct gctccatcag caggtatgcg
      901 gcttccgcgt tggtcgtgtt gccgaccgcg acggggatcg gcatgaagcg cgtgaactcg
      961 gcgagggaga gcgggtcgta gccggtcgcg aggtggcgcg ccgagctcac ctgcgactgg
     1021 accaggaaca ggtccgcgcc gtgctcggcg cagaacggac cccagcgacg cgccgcaccg
     1081 ggcgtggcag ccacggctgc cttcgagccc gccgcgtgga gctcttcgat gcgtttcgcg
     1141 accaggtcgt cgcggaccgg cgccgcgtac gcctccgaga gaacgtcctg gacctcgtcg
     1201 tcggacgcgg cggcgatccg atcgagcacc tcggccggct gctcgtagcg cgtctggacg
     1261 ccttcgaggt tgagcaccgc cagaccgccg aggcgcgcca gcgcgccggc aaagccgggg
     1321 tcgacgaccg cgtccatcgc cgcggcgagg accgggatgg cgaggtcgat gccgcagaac
     1381 gactgcccga gctcgacgtc gttgggctcg atcgtctcga tgccgggtgc gagcgacacg
     1441 tcctcgaagc catacgtcgg ccgcagccgg tcgaccttcg agtcgaacgc agggagcgca
     1501 cgctgctgcg ccggactcgc ctcctgctcg atcaccgggc ggattctagc agcgaggcac
     1561 cctcaggtcg ggtcgggcag ccggtagacg gcggctctgt ccgggttggc gacgagccac
     1621 tcgagctgct cgatcgcgcg gtggtagctc ggccacgtga cgagctcgtg ggcttcggcc
     1681 ggcgtgagcc agcgcaggtc gtcgtgctcg tcggagatcg tgatcgcagc gtccagtcgc
     1741 agccgcgccg cgaagaccgc ctcgctcatc aggccgtcga ccgccacctc gtggaaccag
     1801 ttgacgatgt ccgtctcgaa cagggcctcg atcgcggccg gccccatgcc ggtctcctcg
     1861 gcgacctcgc gcagggcgcc cgcgacaatc gtctcgccgg cctcgagccg gcctgtgacg
     1921 cactgccaca gccccgggta catgcggccc ggcgcgcggt ggatcagcag gatctcgagc
     1981 tgaccgtcgc cggtgacgcg atagagccag caggcgacga ggtccggtcg gagggggctc
     2041 atggcccgag cctatcgccg gggccgatcg agggcccggc tagactcccg cgcatcgtga
     2101 cctggcatcg tgccgccccc ctcgacgagc tccgcgccgc gcgtccatgg ctgcccctga
     2161 cgatcgaggg ggtcgacctc gtggtcgccg tcgtcgatgg cgtgccgtat gccgtggagg
     2221 atcggtgcgc gcacgcgggc tgtgcgttca gcgaggatgc ggacctggtc ggcgacgaca
     2281 tcgtctgcaa ctgccacggc tccgagttcg acctgcgaac aggcgcgctg aagcgcgggc
     2341 cggccgagcg tccggtccgg tcgatccccg tgcgcatcga gggcgagcac ctcgaggtcg
     2401 cgctgtgatc gacctt
//
LOCUS       DATBSW010000104         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_325895, whole genome shotgun sequence.
ACCESSION   DATBSW010000104 DATBSW010000000
VERSION     DATBSW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325895"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2027
                     /locus_tag="VIF63_02960"
     CDS             <1..2027
                     /locus_tag="VIF63_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8238371.1"
                     /translation="LLGAGGVTDLRIVGIVPGFGPLPGPGRTVIIPIDVARSTFGLEG
                     ATRVDLDLADGALATVKADLEAKMVEPYVLASPADLALSLRASSAAFQGTAALVAAIV
                     LFAGAFLIINTLSMTVGERAREVGLLRAAGATRLQLGRFVFSGALLLGVIGAAFGALI
                     GAGLAVAMAGAVSQATGLVPDVAELDVAGVLTAALVGIGITILAAVEPALAAARISPV
                     EALRARLDLPTLQRGRLSWIAFVFLLVAALAMLAWPPVIAASGAERAFAVYGVLLAVT
                     LASPFILRPLARLLGMPVALILRIEERLARGSLARDRSRTTLTLGSLVIGLAMIVALG
                     WSAQAARASAFAWLEDVVPGDELVSSIYPIGDDNPARDALADVPGVARVTPIASFDLA
                     FRGLRVDAAAVVGADFLADGRLTVLQGDRTTALTKLDAGGYAILPAGLARSLELGVGN
                     TMSIPIDSEHQLELTVAAIVERSMPGTSGEAVLVGWKDATESLGVLGADAFAVRFEPG
                     AEEASAPLLAETARTFALEANPISSIQGAIAEALARVFTVFDALAVVAVIVAALGIVN
                     TLTMGVVERIREIGVLRAIGMTRRQVMRMVVVEATILGFVGVILGSLAGIGAGAMLLQ
                     LGGGLGTPGGIPWASIGIAAVLGLVLPALAAVYPARLASRIAIVDSLRYD"
BASE COUNT          243 a          878 c          750 g          373 t
ORIGIN      
        1 cgctcctcgg cgcgggcggc gtcacggacc tccggatcgt cggcatcgtc cccggcttcg
       61 ggccgctccc gggccccggc cggaccgtga tcatcccgat cgacgtcgcc cgctcgacgt
      121 tcggcctcga gggcgcgact cgcgtcgacc tcgacctcgc cgacggcgcg ctcgcaaccg
      181 tgaaggccga cctcgaggcg aagatggtcg agccgtacgt gctggcctcg cccgccgatc
      241 tcgccctcag cctgcgcgct tcgtccgccg cgttccaggg gaccgctgcg ctcgttgccg
      301 cgatcgtcct gttcgcgggc gcgttcctga tcatcaacac cctgtcgatg accgtcggtg
      361 agcgggcccg cgaggtcggg ctgctccgcg ccgccggcgc gacgcgcctc cagctgggtc
      421 gcttcgtctt cagcggcgca ctgctcctcg gcgtcatcgg cgccgcgttc ggtgcgctca
      481 tcggcgccgg gctcgccgtg gccatggccg gtgccgtcag ccaggcaacg ggcctcgtcc
      541 ccgacgtggc cgagctcgat gtcgcgggcg tcctcaccgc ggcactcgtc gggatcggga
      601 tcacgatcct cgccgcagtc gaaccggcac tcgccgccgc ccgaatctcg ccggtggagg
      661 cactccgagc ccgcctcgac ctgccgacgc tccagcgcgg ccgcctgtcg tggattgcct
      721 tcgtcttcct gctcgtcgcc gcgctcgcga tgctcgcctg gccgccggtc atcgccgcgt
      781 cgggcgccga gcgcgcgttc gccgtctacg gagtcctgct ggccgtgacg ctcgcctcgc
      841 cgttcatcct ccggccgctg gcgcgcctgc tcggcatgcc ggtggcgctc atcctgcgca
      901 tcgaggagcg gctcgcccgc ggctcgctgg cccgcgaccg gagccgcacg acgctcaccc
      961 tcggctcgct cgtgatcggc ctggcgatga tcgtcgccct cggctggtcc gcccaggccg
     1021 cgcgtgcctc cgccttcgcc tggctcgagg atgtggtccc cggcgacgag ctcgtcagct
     1081 cgatctaccc gatcggcgac gataacccgg cgcgtgacgc gctcgccgac gtgcccggcg
     1141 tcgcccgggt gaccccgatc gcatccttcg acctcgcatt ccgaggcctg cgcgtcgatg
     1201 ccgcggccgt cgtcggcgcc gacttcctgg ccgatggccg gctcaccgtc ctccagggcg
     1261 atcggacgac tgcgctcacg aagctcgacg cgggtggcta cgcgatcctg cccgccggcc
     1321 ttgcccgcag cctcgagctg ggcgtcggca acaccatgtc catcccgatc gactccgagc
     1381 atcagctcga gctcaccgtc gcggccatcg tcgaacgctc gatgcccggg acgagcggcg
     1441 aggcggtcct cgtcggctgg aaggacgcca ccgaatcgct cggcgtcctg ggcgccgatg
     1501 ccttcgccgt ccgcttcgag cccggtgccg aggaggcttc cgcaccgctc ctcgccgaga
     1561 cagcccggac gttcgccctc gaggccaacc cgatctcgag catccagggc gcgatcgccg
     1621 aagccctcgc tcgcgtcttc accgtcttcg acgcccttgc cgtggtcgcg gtcatcgtgg
     1681 ccgcgctggg catcgtgaac acgctcacga tgggcgtggt cgagcgcatc cgcgagatcg
     1741 gcgtcctgcg ggccatcggc atgacccggc gccaggtgat gcgcatggtc gtggtcgagg
     1801 cgacgatcct cggtttcgtt ggcgtgatcc tcggctccct cgccggcatc ggcgcgggcg
     1861 cgatgctcct ccagctcggt ggcggcctcg gcacgcccgg gggcatcccg tgggcctcga
     1921 tcggcatcgc ggccgtgctc ggcctggtcc tgccggcgct tgccgcggtt tatccggctc
     1981 gcctcgcatc gcggatcgcg atcgtcgact cgctccgcta cgactgatcc ttcgccccgc
     2041 tagggcccgg accgggcacg gaagcgagaa tcttgtctgg tagactcggc cctcgcgagc
     2101 cgcccccatc gatcggcgct tgcgcctcgg gtcgccgagg gcgaggagga catccgcgaa
     2161 tgacgatcgg catgccgcgc atggatcgcc tgcccgccca ggagcgcggc ctgacgatcg
     2221 gtgacttcct cgtcccgatc cgcg
//
LOCUS       DATBSW010000105         4876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_643245, whole genome shotgun sequence.
ACCESSION   DATBSW010000105 DATBSW010000000
VERSION     DATBSW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643245"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VIF63_02965"
     CDS             <1..243
                     /locus_tag="VIF63_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY8238372.1"
                     /translation="SPTHILVVPREHIPSAADLTEDDATTLGRIFATAADIARAEGIA
                     EGGYRIVTNVGTWGGQTVDHLHFHLMGGRAFSWPPG"
     gene            240..785
                     /locus_tag="VIF63_02970"
     CDS             240..785
                     /locus_tag="VIF63_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238373.1"
                     /translation="MSVHRAIRALRSLLVPAVLVLSASACVTTLNTFPPLGSTPKPAG
                     EGSAAVVTQVVGALDARGLQAAESIRPYRPVEGPLVAAAPRTIVQVTLPEDPDHGFIV
                     IYALPSPDAAQDAAEDQAGYVAAGIGGGILNPPGTEYALQVVGSSVVFFSWLPATSPD
                     PRTKEIAEVLATLGVAVPIAG"
     gene            complement(847..2529)
                     /locus_tag="VIF63_02975"
     CDS             complement(847..2529)
                     /locus_tag="VIF63_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8238374.1"
                     /translation="MLSPTSSEHRSPETLLDLVDDAVTRFADQPALAIRREDGTTEGW
                     TYRELDRRSRAAAFRLRALGLNPGDRLLTWSPSTPELPATYLGAMRAGIVLVPLDLRM
                     SKEAIDGIVASSGATHLVIGTGRDAPNPAEFGLDGVPTSTVDVIAAAPDRDGGLPAEW
                     ETQVAAWPRPKPADLVELIFTSGTTGTPKGVMLAHDNLLAGNRTFHRIIPPMEHRIVS
                     LLPLSHLLEQAVGLYYALSVGASVLYVRSRNPRVIFDALREHRVTSMVVVPQVLDLFW
                     SAIEREVEKSGRAALFGRLRSIARRLPMAVRRLIFRRVHAQLGGSFRLFCSAGAFLPP
                     ALQQAWEDMGVTVLQGYGATETGTGCCTSLDDHGLGTVGRPPDGVEMRLADDGEVQFH
                     GATLFKGYWNAPDKTAAAFTADGWYMTGDVGHFDRAGRLILSGRKRDLIVLPNGFNVY
                     PEDLENALRIAGLRDTVVLESKPGRIEAIVLAPSASPVRTAGETLDFDPANPPPELRK
                     TLDAAVKAANASLGPNQHIAGWRLWPESDFPRTHTLKVKRDAVRTWVGVNAI"
     gene            2661..3464
                     /locus_tag="VIF63_02980"
     CDS             2661..3464
                     /locus_tag="VIF63_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238375.1"
                     /translation="MRLDHVYGTLAGFTDEELRAFIRADRVRRSRLERAADQVFMGWL
                     VRAFDRSQLSEARAIASAAIREAGLPEPHPIRAVAAGLLPPLATFVAVFAMAITSGSL
                     FIRGLPIPAADRAMLAEGWASLGLGTAALLAALVTHGALRAGPRDPADVFGERIFGAL
                     RTLYLVVAGLALLPVTFITAVMAVPDLAELTTIILSVVAVVLLVAGIRAGLQTRKLNG
                     VWRPFEDAALAAVAHGHISAFEERLLTQPLETAMQVTAVPAGELRATAT"
     gene            3554..4477
                     /locus_tag="VIF63_02985"
     CDS             3554..4477
                     /locus_tag="VIF63_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8238376.1"
                     /translation="MAGQRVAVTGSFGFTGRALTERLLAAGHDVVTLSRRSGAGDPLA
                     TRITVRPLEATKPDELTASLAGVETLFNTYWLRFPRRGQTFEAAVARSAVLLAAAREA
                     GVRRVVHVSVVNATADAETPYVRAKAALESVVRSAGMEWVIVRPTLTYGPGDILINNL
                     AWALRRLPVYGMPGFGRYTVQPVHVDDVARICQEAAGGEPGRVLDAAGPETLEYRQLV
                     ALIREAVGSRSLVLPMPGPLVLAAARILGLVVRDVVLTADEIRELTSSLLTSTQPPLG
                     EIRLSDWVRANAQTLGRRWSSELDRNYRLAG"
     gene            4637..4822
                     /gene="rpsU"
                     /locus_tag="VIF63_02990"
     CDS             4637..4822
                     /gene="rpsU"
                     /locus_tag="VIF63_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY8238377.1"
                     /translation="MAEVRVGENETFESALRRFNKKIQQSGILAEARRREHYEKPSVK
                     RKRKEAKRKKNARTPQG"
BASE COUNT          694 a         1723 c         1724 g          735 t
ORIGIN      
        1 tcgccgaccc acatcctcgt cgtgccccgc gagcacatcc catccgcggc ggacctgacc
       61 gaggacgacg cgacgaccct cgggcggatc ttcgccaccg ctgccgacat cgcccgagcc
      121 gagggcatcg ccgagggcgg ctaccgcatc gtgaccaacg tcggtacgtg gggcggccag
      181 acggtcgacc acctccactt ccacctcatg gggggccgcg cgttcagctg gccgcccggg
      241 tgagcgtcca ccgggccatc cgggcgcttc ggtcgttgct cgttcctgcc gtgctcgtcc
      301 tttccgcctc ggcctgcgtc accacgctga acacgttccc gccgctcggc tcgacgccga
      361 agccggccgg cgaaggctct gcggcggttg tcacgcaggt cgtcggcgcg ctcgacgcgc
      421 gcggcctcca ggccgccgaa tcgatccgtc cgtatcgacc cgtcgaaggc cccctcgtcg
      481 ccgcggcgcc gcgaaccatc gtccaggtca ccctgcccga ggatccggat cacggcttca
      541 tcgtgatcta cgcgctgccg tcgcccgacg ccgcacagga cgcggccgag gatcaggcgg
      601 ggtacgtcgc ggccgggatc ggcgggggca tcctgaaccc tcccggaacg gagtacgcgc
      661 tccaggtcgt cggctcgtcg gtggtcttct tcagctggct gccggcgacg tcgcccgacc
      721 cgcggacgaa ggagatcgcc gaggtgctcg cgacgctcgg cgtcgcggtg ccgatcgcgg
      781 gctaggcgat ccgggcgggc cccgggaccg cgggcctcga gatcgcgggc ccctagatcg
      841 cgggccctag atcgcgttca cgccgaccca ggtgcggacc gcgtcgcgct tcaccttgag
      901 cgtgtgcgtc cgcgggaaat ccgactcggg ccagaggcgc cagcccgcga tgtgctggtt
      961 cgggccgagg ctggcattcg cggccttgac cgccgcgtcg agcgtcttcc ggagctccgg
     1021 gggcggattg gcggggtcga agtcgagcgt ctcgcctgcc gtgcgcacgg ggctcgcgct
     1081 cggcgcaagg acgatcgcct cgatgcgacc gggcttcgac tcgagcacga ccgtgtcgcg
     1141 caggcccgcg atcctgagcg cgttctcgag gtcctccggg tacacgttga agccgttggg
     1201 gagcacgatc aggtcgcgct tgcgacccga caggatgagc cggccggcac ggtcgaaatg
     1261 cccgacgtcg ccggtcatgt accagccatc ggcggtgaac gcggcggcgg tcttgtctgg
     1321 cgcgttccag taacccttga acagggtcgc gccgtggaac tggacctcgc cgtcgtcggc
     1381 gagccgcatc tcgacgccgt cgggcggacg accgacggtg cccagcccgt ggtcgtcgag
     1441 tgaggtgcag caacccgtgc ccgtctcggt cgcgccgtag ccctggagga ccgtgacgcc
     1501 catgtcctcc cacgcctgct ggagcgccgg cgggaggaac gcaccggccg agcagaacag
     1561 gcggaacgac ccgccgagct gcgcgtggac cctgcggaag atcaggcgcc ggacggccat
     1621 cggcagccgg cgggcaatcg atcgaagccg gccgaacagg gctgcccggc ccgacttctc
     1681 gacctcgcgc tcgatcgcgc tccagaacag gtcgagcacc tgcggcacga cgaccatcga
     1741 ggtgacgcgg tgctcgcgca gggcatcgaa gatcacccgc ggattccggc tcctgacgta
     1801 caggaccgac gcgccgaccg agagcgcgta gtacagcccg accgcctgct cgagcaggtg
     1861 cgagagcggg agcagcgaca cgatccgatg ctccatcggc gggatgatcc ggtggaacgt
     1921 tcggttcccg gcgagcaggt tgtcgtgggc gagcatcacg cccttcggcg tgccggtcgt
     1981 gccggacgtg aagatcagct cgacgaggtc ggccggcttc gggcgcggcc acgcagcgac
     2041 ctgggtctcc cattcggcgg gcagcccgcc gtcgcggtcc ggcgcggcgg cgatgacatc
     2101 gacggtcgac gtaggaacgc cgtcgaggcc gaactcggcc ggattgggcg cgtcgcggcc
     2161 ggttccgatg acgaggtgcg tcgctccgga gctcgcgacg atgccgtcga tcgcctcctt
     2221 cgacatgcgc aggtcgagcg gcaccaggac gatgcccgca cgcatcgcgc cgaggtaggt
     2281 cgccgggagc tcgggggtcg agggcgacca ggtcagcagt cgatcgcccg ggttgaggcc
     2341 gagcgcgcgc agtcgaaacg cggcggcgcg cgatcgccga tcgagctcgc gataggtcca
     2401 gccctcggtg gtgccgtcct cgcgtcggat ggcgagggcg ggttggtcgg cgaatctcgt
     2461 caccgcgtcg tcgacgaggt ccagcagcgt ttccggcgac cgatgctcgg acgaagtggg
     2521 actcagcatc gcgacgatca ttgcatctct gctcacgcga gaggatcgcg agtcgcgcgt
     2581 agtcggttcc ggtgcggtgg cgaggggttt ccgatgcctg ggtgcaccag agtgcccttg
     2641 tgaggggtaa tactcccgtc atgcgactcg accacgtcta cgggaccctc gccggcttca
     2701 cggacgagga gctgcgcgcc ttcatccgtg ccgatcgcgt tcgtcggagc cgcctcgagc
     2761 gcgcggccga ccaggtcttc atgggctggc tcgtgcgcgc cttcgatcgg agccagctca
     2821 gcgaggcgcg agccattgcc tccgcggcga tccgcgaggc tggtctcccg gagccgcatc
     2881 ccatccgcgc agtcgcggcg ggcctgttgc caccactcgc gaccttcgtg gcggtcttcg
     2941 cgatggcgat cacgtccggc tcgttgttca ttcgcgggct gccgatcccg gcggccgatc
     3001 gggcgatgct ggccgagggg tgggcgtcgc tcgggctcgg caccgccgcg ctgctcgccg
     3061 cgctcgtaac ccacggcgcg cttcgcgccg gcccgcggga tccggccgac gtgttcggcg
     3121 agcgaatctt cggtgcgctg cggacgctgt acctggtcgt cgccggcctc gcgctgctgc
     3181 ccgtgacgtt catcacggcc gtcatggccg tgccggatct cgccgagctg accacgatca
     3241 tcctgtccgt cgttgccgtc gtgctcctcg tcgcggggat ccgagccggg ctgcagacgc
     3301 ggaagctgaa cggggtctgg cgacccttcg aggacgccgc cctcgctgcc gtcgcccacg
     3361 gccacatcag cgcgttcgag gagcgcctgc tcacgcagcc actcgaaacc gcgatgcagg
     3421 tcaccgcggt cccggccggg gagctccggg caaccgcgac ctagcggcgg cactcgaccg
     3481 ggtcgcgttc cgggctgatg cccttcgtgc tactgtgcgg tagcacatta agcccgccac
     3541 ccaggagcag ccgatggctg gacagcgcgt cgccgtgacc ggctcgttcg gatttaccgg
     3601 gcgcgcactc accgaacgac tgctcgcggc cggacacgac gtcgtgaccc tctctcgacg
     3661 ctcgggcgcc ggtgacccgc tcgcgactcg gatcaccgtc cgaccgctcg aggcgacgaa
     3721 gccggacgag ctcacggcga gcctcgccgg cgtcgaaacg ctgttcaaca cgtactggct
     3781 gcgcttcccg cgacggggtc agactttcga ggccgcggtc gcacgttccg cggtgctgct
     3841 cgcagcggcg cgcgaggctg gcgtccggcg ggtcgtccac gtgagcgtcg tcaacgcgac
     3901 ggccgatgcc gagacgccgt acgtccgcgc caaggccgcg ctcgagtcgg tcgttcgatc
     3961 ggcgggcatg gagtgggtga tcgtccgccc gacgctgacc tacgggccgg gcgacatcct
     4021 gatcaacaac ctcgcctggg cgctgcgccg gctgccggtc tacggcatgc cggggttcgg
     4081 gcggtacacg gtccagcccg tgcatgtcga cgacgtcgcg cggatctgcc aggaagcagc
     4141 tggaggcgag ccgggccgcg tcctggatgc ggcaggcccg gagacgctcg agtatcggca
     4201 gctcgttgcg ctcattcgtg aggctgtcgg cagccgctcg ctcgtcctgc cgatgcccgg
     4261 tccgctcgtc ctcgccgcgg cgcggatcct gggcctcgtc gtccgcgacg tcgtcctgac
     4321 cgcggacgag atccgcgagc tgacgtcctc gctgctgacc tcgacgcagc cgcccctcgg
     4381 cgagattcgc ctctccgact gggtccgggc caacgcgcaa accctcggcc gccgatggtc
     4441 cagcgagctg gatcggaact accgactcgc cggataggcg ccccggaatc gtgctcgatc
     4501 acgatcgcgc cgacgtttga ccccttttgg ggcctgtgcc acactccggc ggcccttttg
     4561 ggggaccgcg tcgacgccgt gggcgacgag cacgatgtgg ggccgaagac aagcgcccga
     4621 ggaggtgaat cccggattgg cagaagttcg agtgggtgag aacgagacgt tcgagagcgc
     4681 cctccgccgc ttcaacaaga agatccagca gtccggcatc ctcgccgagg ctcgccgacg
     4741 cgagcactac gagaagccga gcgtgaagcg gaagcgcaag gaagcgaagc gcaagaagaa
     4801 cgcgcgaacg ccacagggct gatcgaccgg acggccggga ccgtccgacg cgcagcatcg
     4861 cgatcgacgt tccgcc
//
LOCUS       DATBSW010000106         8449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_8567, whole genome shotgun sequence.
ACCESSION   DATBSW010000106 DATBSW010000000
VERSION     DATBSW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8567"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VIF63_02995"
     CDS             complement(<1..662)
                     /locus_tag="VIF63_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8238378.1"
                     /translation="MSEPPDLPARPLAGRVALVTGAGSGIGRATAIRLALDGAAVGCL
                     DRNAAGADETVAAIVASGGQAAALYADVTNSGEVAAAATRIGDELGPASILVNNAGLA
                     GAGRFVDVSLEDWQRVVDSHVLGAFLVTRAVLPAMVAGGWGRIVLIASEAVWLGHTSV
                     QYATAKAALVGFTRSLARQVAAQGVNVNAVAPGPVETPMFLDNTPDEIEAERRSVLIG
                     RVL"
     gene            complement(1172..4471)
                     /locus_tag="VIF63_03000"
     CDS             complement(1172..4471)
                     /locus_tag="VIF63_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8238379.1"
                     /translation="MPAKFSSERFVGRERELSHLAVALEAAADGRSPRLLVSGRGGVG
                     VSRLVTEAVRRVTRLSRPFQVVRCSAVSARGRAAFGPIIEGFAPWLEALDDAELRRAV
                     GSGAEPIARLIPALAPRLVGEVHHARRDSISPERRGAWIGEAIQGLLERAGERQPILL
                     MLEDLHHADAGTRNLATFLARVARPARVCVLLTYGRDRLTRGHPLQAQLGAITSAADP
                     PTQLELGPLDRFDLAQLVTEIEGERPTAAALLLVAERSGGDPLIAEEVLAARRELPGL
                     SLGSTLDELVLARLARRAAEARRVLRLLAPAGRPVDRGELAAIALTFEDLVEGLPPRS
                     TTRPRRGDGVLDADLRAGVTEALDHGFLVERTDERLELRHELVAQAIEADLLPVQRRR
                     HHLALAAALSADPASSLTHWLAGYEPARARSAALAAASTAEKLDSAADALAARELALE
                     LGAASGARAADGKLLYETAQVALAAGRPDRALAYLESAAGRFGEREDAAMAASIYEML
                     GRASRTLGNHDRALAEHRRAAAIAPDDDAPLRASVLASLAQTLMLLGHFAEATRVGSE
                     AIAVARAVGGDALATEAHALCTVGVANAWGARGSEGITQLQEALQLARDLDDPDVAFR
                     ASLNLSTALALFGRRDEAIDVTRDAIDRARTDGLEVAYGNSLRGNIAEALFNAGRWTE
                     ARETIRTALEWSPDPVAFADASVTAAMLEVETSVDERAASLLGWRPLEIDQAPDPQLE
                     VPATRAAASFALWRGDVADARRAVERGWALVRRAEDWALAARMAATYLEVQAAIATDA
                     RERRALSEISGARQRGRRVLVEAETVLRSSGIAQGSPSRLEADANLATARAYAGRLDG
                     SDDPVLWDAAAQGWERAREPYQVARARWRQAEAALPGGHDARVARAVARGPLLEAARI
                     ARELGARPLLRELTNLASRALITLPDAEPAETTVVTTTGRAGIPVIRGTAAARAAAAA
                     ATHGEPIASRVPADGAGNGATDGAAAAVTDGPTGITAAFAPEDVANRPKEAFGLSRRE
                     REVLALIAEGRTNREIGERLFISQKTVGVHVGNILSKLGASGRVEAAMVAIRLELVPT
                     PLAGRPVLAAT"
     gene            4537..5895
                     /locus_tag="VIF63_03005"
     CDS             4537..5895
                     /locus_tag="VIF63_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238380.1"
                     /translation="MIEPKSPLPDDTARGAEPDDDAPASPLWQNRSFLAVWTASTVSI
                     FGSLITRTALPFAAILFLDAGPVEISWIRSAELVAGLVFGLAAGAWVDRLRRRPILIW
                     ADLGRALILASIPIAYLANALSLLQLVLVAFATAVLSTFFDVADHAYLPTVVSRKRLV
                     DANSALTATSSVAEFSAFGLGGFLIQAFTAPIAIAIDAVSFVVSAILLATIRKPERPP
                     TPHADREPVLREIADGIRILARSPLLRALALSHGGTHILWGIFGTSYLIFANKELGLD
                     PATIGVIAAIGGIGSLAGSAMAPIMVRRFGIGNSILLGIVGFIAGNALIPLAPAAGAV
                     VGIPLGALFLIGQQLLGDSLATVFEVTETSLMQASVSDRVLGRVNASFGTFTTLLTLL
                     GAVAGGYIAETWGLRTAFWVGLAGAALTLIVVWFSPVRHVREAPLVPMIDMPGDTLPI
                     TE"
     gene            5899..6831
                     /locus_tag="VIF63_03010"
     CDS             5899..6831
                     /locus_tag="VIF63_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY8238381.1"
                     /translation="MPPHPRNTLNELSGEEWLYFTKSVWTTAYPSELGHALRKQHGAN
                     KPPRLIARLIEFFTKSAELVLDPFAGVGGTLLGAAIARGPRRALGIELSPRWAEVYAE
                     VVAAALAERDGSGPVLADLGTSDPGGPRTFDPAGLELRVGDALQLLPSLDPESVDFVA
                     TDPPYNVQLPMTMSGGALSKDFTNRRTDYAMVTNDPADLANADDYPAFLDGMTAVLAE
                     LQRILRPSRYAVLIVRDAYQDGRYLFTGADLAARAASVGLVPKGDLIWYQAGTRLRPY
                     GYPTGFVPNIAHQHLLVFRREPARAKAARRPTNR"
     gene            complement(6930..7634)
                     /locus_tag="VIF63_03015"
     CDS             complement(6930..7634)
                     /locus_tag="VIF63_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238382.1"
                     /translation="MQGIIRAIARLLAMGLVMAAVLAGAVFMGFGAVTAIAMGIVVAG
                     VVGLVGVARSSAGPNQSDHDQIAAMMKPHVPAPTPAARPGIGTEVEAMAPRVEAPLNP
                     EANMPRWRRPSLLEARHSDPTLTAPVYRLPMRFGDTDLSNLDLRVVRYAVVPVLDRPD
                     EVLGLQVSDLSSGDEVVVVNSSGAFIEVMCPNGDQGWVHRTTLGKIGAVGMDNRHVPQ
                     EADDALTALLTARGLI"
     gene            7887..>8449
                     /locus_tag="VIF63_03020"
     CDS             7887..>8449
                     /locus_tag="VIF63_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238383.1"
                     /translation="MQVLASNRSRAGRPTRLGVANALFMGTILVSTSATLLYLVFGEN
                     FVDRFMPSGRPSTYELVAGALAWTFALTAPAGFGLVGVARLVTAYDRWRARRPRVTPA
                     LRLRRAIGDDHVVATGVRLPDGSRVLPELVFGPFGAAILEELPPVGAVMSRGVRSWEV
                     RVGNGQIRTIENPLLLAAHDAERVRSWL"
BASE COUNT         1129 a         3074 c         3019 g         1227 t
ORIGIN      
        1 aggactcgtc cgatgagcac cgatcttcgt tccgcctcga tctcgtcggg cgtgttgtcg
       61 aggaacatcg gcgtctcgac ggggcccggg gcgaccgcgt tgacgttcac gccctgggcg
      121 gcgacctggc gagcgagcga gcgggtgaag ccgacgagcg ccgccttggc ggtggcgtac
      181 tgcacgctcg tgtggcccag ccagacggcc tccgatgcga tcagcacgat ccggccccag
      241 ccgcccgcga ccatcgccgg caggaccgct cgtgtcacca ggaacgcacc caggacatgg
      301 ctgtccacga cgcgctgcca gtcctcgagg ctgacgtcga cgaaccgtcc ggcgcccgca
      361 aggccggcgt tgttcacgag gatgctcgct ggcccgagct cgtcgccgat gcgggtcgcg
      421 gcagcggcga cctcgccgga gttcgtgaca tcggcgtaca gcgccgcggc ctgaccgccg
      481 gacgcaacga tcgcggcaac ggtctcgtcg gccccggccg cgttccggtc gagacagccc
      541 accgcagcgc catcgagagc gagccggatg gcggtcgcgc gaccgatccc ggacccggcg
      601 ccggtgacca gggcgacgcg cccggccagc ggtcgagcgg gcaggtcagg gggctcggac
      661 atgcccgcga tggtgccacg gcaccgaaag tccggtgcgt ttccacaccg aaataagtgc
      721 cagataagcg gtcatcgata ccgtcctgcc cccagccagg tgcggctcgg tggagtcagg
      781 aatcgaatgg tgcggttggg gcctcggggt cggcaccgat ggtcctagtc ggacccctga
      841 ctttggtcgc atcgactcga tcggggcctg ttcgtacgtt tgccgccctc ggtcgacgag
      901 accactgccc aggaatactg cccaacgttt cgaggagaaa cacatcatgg ccgatcgact
      961 tcgtcgtgcg tggaccttcg tcctggccgt cgcccccatc gcggcgattg ccctggtcga
     1021 agccgccatg cggcgctggt aggaacccac accacagaca tccccggctg accggcccct
     1081 tcctcccggt cacgccagtc gcatcgaaca ggacccggat cctcctccgg gtcttgttcc
     1141 ttttccggcc gaaattgcgc cgcgtggagc gctaggtcgc agcgaggacc ggccgcccgg
     1201 cgaggggcgt gggcacgagc tcgaggcgga tcgccaccat cgcggcctcg acccggccgg
     1261 acgcgccgag cttcgacagg atgttcccga cgtggacgcc gacggtcttc tggctgatga
     1321 acagtcgctc gccgatctcg cggttggtgc gaccttcggc gatcagcgcg aggacctcgc
     1381 gctcgcggcg tgacagcccg aaggcctcct tcggccggtt cgcgacgtcc tccggggcga
     1441 acgcggccgt gatgcccgtc gggccgtccg tgacggccgc ggcggcgccg tccgtcgcac
     1501 cattccctgc tccatctgcg ggcacgcgcg aggcgatcgg ctcgccgtgc gtcgcggcag
     1561 ccgcggccgc acgcgcagcg gctgtgccgc ggatgacggg gatgccggct cggccggtcg
     1621 tcgtcacgac ggtggtctcc gccggctccg catcaggaag cgtgatcagc gcgcgactcg
     1681 cgaggttggt cagctcgcgc aggagtggcc gcgcgccgag ctcccgtgca attcgcgccg
     1741 cctccaggag cggtccgcgg gcgaccgctc gggcgacccg ggcgtcgtgg ccgccgggga
     1801 gggcggcctc ggcctggcgc cagcgagcgc gggcgacctg gtacggctcg cgggcgcgct
     1861 cccagccctg cgccgccgcg tcccatagga ccgggtcgtc gctgccgtcg aggcggcctg
     1921 cgtaggcgcg ggcggtggcg aggttcgcgt ctgcctccag gcggctgggc gagccctggg
     1981 cgatgccgct cgagcgcagc acggtttcgg cctcgaccag gacgcggcgc ccacgctggc
     2041 gcgcgccgct gatctcggac agggcgcgtc gctcgcgggc atcggtcgcg atcgcggcct
     2101 ggacctcgag gtaggtggcg gccatgcgtg ccgcgagggc ccaatcctcg gcgcgccgga
     2161 ccagtgccca gccgcgctcc acggcccgcc gggcgtcggc cacgtcgccg cgccagaggg
     2221 cgaacgaggc ggcggcgcgg gtcgcgggca cctcgagctg ggggtcgggc gcctggtcga
     2281 tctcgagcgg ccgccagccg agcaggctcg cggcgcgctc gtcgacgctc gtctcgacct
     2341 cgagcatcgc ggcggtcacg gacgcgtcgg cgaaggcgac cgggtccggg ctccactcga
     2401 gggcggtgcg gatcgtctcg cgcgcctcgg tccagcggcc ggcgttgaac agggcctcgg
     2461 cgatgttgcc gcgcagcgag ttgccgtagg cgacctcgag gccgtccgtg cgggctcgat
     2521 cgatcgcatc gcgggtgacg tcgatcgcct cgtcccgccg cccgaacagg gcgagcgcgg
     2581 tcgagaggtt gagggaggcc cggaacgcga cgtcgggatc gtcgaggtcg cgcgcgagct
     2641 ggagcgcctc ctgcagctgg gtgatgcctt cgctgccccg cgcgccccat gcgttggcca
     2701 cgccgacggt gcacagggcg tgcgcctcgg tcgccagcgc gtcgccaccg acagcgcggg
     2761 cgacggcgat cgcctcgctc ccgacccgcg tcgcctccgc gaagtgaccg agcagcatca
     2821 gcgtctgggc gagcgaggcg agcacggacg cgcggagcgg cgcatcgtcg tccggcgcga
     2881 ttgcggcggc tcgtcggtgc tcggcgaggg cgcggtcgtg gttgccgagg gtgcgcgagg
     2941 cccggccgag catctcgtag atcgaggcgg ccatcgccgc atcctcacgc tcgccgaagc
     3001 gacctgcggc gctttccagg taggcgaggg cgcggtccgg ccggcctgcc gcgagggcca
     3061 cctgggccgt ctcgtacagg agcttgccgt cggcggcccg ggcgccgctg gcggcgccga
     3121 gctcgagggc cagctcgcgg gcggcgaggg catcggcggc ggaatcgagc ttctccgcgg
     3181 tcgaggcggc ggcaagggcg gcggagcggg cgcgggccgg ttcatagccc gcgagccagt
     3241 gcgtgagcga cgatgccggg tcggcactca gggcggcggc caacgcgagg tggtgccggc
     3301 ggcgctgcac cgggagcagg tccgcctcga tggcctgggc gacgagctcg tgccgcagct
     3361 cgagccgctc gtcggtccgc tcgaccagga agccgtggtc gagcgcctcg gtgacgcccg
     3421 cgcgcaggtc tgcgtcgagg acgccgtcgc cgcgtcgcgg gcgcgtcgtc gaacgtggcg
     3481 gcaggccctc gaccaggtcc tcgaaggtca gtgcaatggc ggcaagctca ccgcggtcca
     3541 ccggacgacc ggccggggcg aggagccgaa gcacgcggcg ggcctcggcg gcacgccgag
     3601 cgaggcgggc cagcacgagc tcatccagcg tggagccgag ggacaggccg ggcagctcac
     3661 gacgggccgc gagcacctcc tcggcaatca gcgggtcgcc gccggagcgc tccgcgacga
     3721 gcagcagcgc cgcggccgtc gggcgctcgc cttcgatctc ggtcacgagc tgggcaaggt
     3781 cgaaccgatc gagcgggccg agctcgagct gagtgggcgg atcggcagcg ctcgtgatcg
     3841 cgccgagctg cgcctggagc gggtggccgc gcgtgagccg gtcgcgcccg tacgtgagca
     3901 ggacgcagac gcgggcgggt cgcgcaacgc gggcgaggaa ggtcgcgagg ttgcgcgtgc
     3961 ccgcatccgc atggtgcagg tcctcgagca tcagcaggat cggctggcgc tcccctgccc
     4021 gttcgagcag cccctggatc gcttcgccga tccacgcgcc acgacgctcc ggggagatcg
     4081 agtcacggcg ggcgtggtgg acctcgccca cgaggcgcgg tgcgagcgcc ggaatcagcc
     4141 gggcgatcgg ctccgcgccg gacccgacgg cacgccggag ctcggcatcg tcgagcgctt
     4201 ccagccacgg cgcgaagccc tcgatgatcg ggccgaaggc agcgcggccg cgggcggaca
     4261 cggcgctgca ccgaacgacc tggaacggcc ggctcaggcg cgtgacccgg cgcacggcct
     4321 cggtcacgag gcggctcacc ccgacgccgc cgcgcccgga cacgagcagg cgcgggctgc
     4381 gaccgtcggc ggccgcttcg agcgcgacgg cgaggtgcga gagctcccgc tcgcgcccga
     4441 cgaatcgttc gctgctgaac ttcgcgggca tggacccatc gtgccacacg gctcccactc
     4501 cacgatcccc gtctgcgcgg ctacccttcc gccgcgatga tcgaacccaa atccccgttg
     4561 cccgacgaca ccgcgcgcgg cgccgagccg gatgacgacg cccccgcgtc accgctctgg
     4621 cagaaccgca gcttcctcgc ggtctggacc gcgagcacgg tttcgatctt tggctcgctg
     4681 atcacgcgga ctgcgctccc gttcgcagcc atcctcttcc tcgatgccgg gcccgtcgag
     4741 atctcctgga tccgatccgc ggagctcgtc gccggcctcg tgttcggcct cgccgccggg
     4801 gcctgggtcg atcgtctccg gcgccgcccg atcctgatct gggccgacct cggtcgagcg
     4861 ctcatcctcg cttcgatccc gatcgcctac ctcgccaacg ccctgagcct gttgcagctg
     4921 gtgctcgtgg cgttcgcgac ggccgtgttg tcgaccttct tcgacgtcgc cgaccatgcc
     4981 tatctgccga ccgtggtctc gcgcaagcgg ctggtcgacg cgaacagcgc cctgacggcg
     5041 acctcctcgg tcgcggagtt cagcgcgttc ggcctcggcg gcttcctcat ccaggccttc
     5101 actgcaccga tcgcgatcgc tatcgatgcc gtctcgttcg tggtctcggc gatcctgctc
     5161 gcgacgatcc gcaagccgga gcggccgccg acgccccatg ccgatcgcga acccgtgctc
     5221 cgcgagatcg ctgacgggat ccggatcctc gcccgatcgc cactgttgcg ggccctcgcc
     5281 ctttcgcacg gcggcacgca catcctgtgg ggcatcttcg ggacgtcgta tctgatcttc
     5341 gcgaacaagg agctcggcct cgatcccgcg acgatcggcg tgatcgccgc gatcggcggg
     5401 atcggatcgc tggcggggtc ggccatggcc ccgatcatgg ttcgccgctt cgggatcggc
     5461 aactcgatcc tcctcggcat cgtcggcttc atcgcgggca acgcgctcat cccgctcgcg
     5521 cctgcggcgg gcgccgtggt cggcatcccg ctcggcgcgc tgttcctcat cgggcagcag
     5581 ctcctcggcg actcgcttgc cacggtcttc gaggtcaccg agacatcgct catgcaggcg
     5641 tcggtgagcg atcgcgtgct cgggcgcgtg aacgcctcgt tcggcacgtt cacgacgctg
     5701 ctcacgctcc tcggcgcggt cgccggcggc tacatcgccg agacgtgggg cctccggacc
     5761 gccttctggg tcggcctggc cggggcggcg ctcaccctca tcgtggtctg gttctcgccc
     5821 gtccgccacg tgcgagaggc gccactcgtg ccgatgatcg acatgcccgg cgacaccctg
     5881 ccgatcacgg aatagcgcgt gccgccccac ccacggaaca cgctgaacga gctctccggc
     5941 gaggagtggc tgtacttcac gaaatcggtc tggacgactg cctacccgtc cgagctgggc
     6001 cacgcgctgc gcaagcagca cggcgccaac aagccgccgc gcctgatagc ccggctgatc
     6061 gagttcttca cgaagtccgc cgagctggtc ctggatccgt tcgcgggcgt gggcgggacg
     6121 ctcctcggcg ccgcgatcgc gcgcgggccg cggcgcgcgc tgggcatcga gctctcgccc
     6181 cgctgggccg aggtctacgc cgaggtcgtc gccgccgcgc tcgcggaacg cgacggctcg
     6241 gggccggtgc tcgccgatct cggcacgagc gatccgggcg ggccgcgcac gttcgatccg
     6301 gcgggcctcg agctgcgcgt cggcgacgcc ctccagctcc tgccgagcct cgatccagag
     6361 tccgtggact tcgtcgcgac cgatccgccg tacaacgtgc agctgcccat gacgatgtcc
     6421 ggcggcgcgc tctccaagga cttcacgaac cgacgcacgg actacgcgat ggtcaccaac
     6481 gacccggccg acctcgcgaa cgcggacgat tacccggcct tcctcgacgg catgacggca
     6541 gtcctcgccg agctgcagcg aatcctccgc cccagtcggt acgccgtgct gatcgttcgc
     6601 gacgcgtacc aggacgggcg ctacctgttc accggtgccg acctggccgc gcgcgccgcg
     6661 agcgtcgggc tggtgcccaa gggcgacctc atctggtatc aggccggaac gcggctgcgg
     6721 ccgtacggct acccgaccgg gttcgtaccg aacatcgccc accagcacct gctcgtgttc
     6781 cggcgagagc cggcccgcgc gaaggcagcg cggcgaccga ccaaccggta gaaatggcgc
     6841 ggcgggccgc ccgggcaacc tgccccgacg acccgccgac ttgaaacgcg ggtgggaccg
     6901 gctcccctgg agccggcaga cggcctgccc tagatcaggc cacgagctgt gagcagtgca
     6961 gtcagggcgt cgtccgcttc ctggggcacg tggcggttgt ccatgccgac cgcgccgatc
     7021 ttgccgaggg tcgtgcggtg gacccagccc tggtcgccgt tcgggcacat gacctcgatg
     7081 aacgcgcccg aggaattcac gaccacgacc tcgtcgcccg acgacaggtc gctgacctgg
     7141 aggccgagca cctcgtccgg gcggtcgagg acgggcacga ccgcgtagcg cacgacgcgg
     7201 agatcgaggt tggagaggtc ggtgtcgccg aagcgcatcg gcaggcggta caccggcgcg
     7261 gtgagggtcg ggtcggagtg gcgcgcctcg aggagcgacg gccggcgcca gcggggcatg
     7321 ttcgcctcgg gattcagcgg cgcttcgacg cgcggtgcca tcgcctcgac ctcggtgccg
     7381 atgcccgggc gggcggcggg agtcggcgca ggcacgtggg gcttcatcat cgccgcgatc
     7441 tggtcgtgat cggactgatt cggcccggct gacgagcgag cgacgccgac aaggccgacg
     7501 acgccggcga cgacgatgcc catcgcgatc gcggtcacgg cgccgaagcc catgaacacg
     7561 gcgcccgcga ggacggctgc catgacgaga cccatggcca gcaatcgggc gatagcccgg
     7621 atgattccct gcatgtggtt ccagtctccc aagcgggtga gatcgccccc gcgatgaatg
     7681 actggagcat cgggtcaggg tgaaggggtc gcaatggtac ggcggtcatg ttggtcctga
     7741 gcgcatcgct gcggtcttgt caggtctggt gcggagcggt tcggtccgga cccctcatcg
     7801 acccgccgga tggctccaca tggcagtacc ttctttgccc cgttcgggtc attcccaccg
     7861 cgcgtcgcgc gccccacgat ccgtgcatgc aagtgctcgc ctcgaatcga tccagggccg
     7921 gccgacccac gcgccttggc gtcgccaacg cgctgttcat gggcacaatc ctcgtgagca
     7981 catcggcgac cctcctgtac ctcgtgttcg gcgagaactt cgtcgatcga ttcatgccga
     8041 gcggtcgccc gtcgacctac gagctcgtcg ccggcgccct cgcctggacc tttgccctca
     8101 ccgcaccggc cggcttcggc ctcgtcggtg tcgctcgtct cgtcaccgcc tacgaccgct
     8161 ggcgcgcacg gcgcccgcgc gtgacgcctg ccctccgcct gcgccgagcc atcggcgacg
     8221 accacgtcgt cgcaaccggc gttcgactgc ccgacggcag ccgggtcctg cccgagctgg
     8281 tcttcggtcc gtttggcgcg gcgatcctcg aagagctccc gcccgtcggc gcggtcatgt
     8341 cccgcggcgt ccgcagctgg gaggtccgag tcggcaacgg ccagatccgc acgatcgaga
     8401 atccgctgct cctcgcggcc cacgacgccg agcgcgtccg cagctggct
//
LOCUS       DATBSW010000107         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_290990, whole genome shotgun sequence.
ACCESSION   DATBSW010000107 DATBSW010000000
VERSION     DATBSW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290990"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VIF63_03025"
     CDS             <1..920
                     /locus_tag="VIF63_03025"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8238384.1"
                     /translation="AVSDDNRIVAVGEEAREMIGRTPGNIHAIRPMKDGVIADYVITE
                     AMLRFFINKAMSGRMRMRLSGPAVMISVPAGVTSVEKRAVKDAALKAGAREAHLIEEP
                     LAAAIGANVPISGPSGNMVIDIGGGTSEVAVIALGGIVVSTSLRVGGNKFDESITSYI
                     RKKYNLMIGERTAEEVKITIGTALPLERELSMEVRGRDLIAGLPRTIPITSSEVMEAI
                     ELPLQQLVAAVRHVLEQTPPELSSDIIDKGMVMSGGGALLRNIDKLLTQVTGVPCHVA
                     ENALNCVALGTGEALKHYDFFKKSLVQTV"
     gene            935..1690
                     /locus_tag="VIF63_03030"
     CDS             935..1690
                     /locus_tag="VIF63_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8238385.1"
                     /translation="MAASAGTDPNAARAFVDLHCHTSASFDSLASPAAVVRAAAARGL
                     THLAITDHDRIDGALEAKAAAAVEAPGLTVLVGQEIRTTAGDLIGLFLHEPIRPGLAP
                     AEAVAAVRAQGGLIGMAHPFDRFRGSVGRGAAVELDELARTVDWIEAWNARLIVGDGN
                     ARAAQLATRAGIAGVAVSDAHTTMEVGIAATILSGDPGTPEGMQAALTGPLELVMGRA
                     SAYVRLFGPAAKLVQRARGRGRVRVAADAASRR"
     gene            1687..2787
                     /locus_tag="VIF63_03035"
     CDS             1687..2787
                     /locus_tag="VIF63_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY8238386.1"
                     /translation="MTEPTQPAPPAAPAPPAAPAPPAEEAEVRAEQLSLASRLRQPRT
                     IVSLVLPIVLLALFVKSLPGFKLEELPDKILGANPLLMLGAFVIFYIGFPLRGLRWAM
                     LVRRTGFPLTVKDATEIIFISWLVNCLVPAKLGDVYRAYLLKINSTVSLSRTFGTVFI
                     ERVLDLFAIVVLGLAAGFVSFRSGLPADVQIVFLIGVVFVLLLAGGLLTMRNFGRRII
                     TALPLPHRVIEFYDRFEEGVFASIGLRALPRLVIVTGLIWATEATRLFLVVQALGLPG
                     VHLGISGAFFVALSASLLTAVPLTPAGIGFVEGGIVGLLTLVYGVAQTDALAITLVDR
                     AISVLSIIVLGSIVYAVSPKRRGMGVQSAAPA"
     gene            2963..3499
                     /locus_tag="VIF63_03040"
     CDS             2963..3499
                     /locus_tag="VIF63_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8238387.1"
                     /translation="MTRTNGRAFNEWLRAQLKAKKMSQRQLAQQSGVDHSTISRLVRG
                     DRMPSLGTATKLARGLRELGENDDGPQYLGLMGGGVQNPTARVEYSLRADESLSEAQV
                     RQVMEYYLAVRMRRIGGAFSNDNSHLQRVEPRDNNTRAFAPPTNSPIVPRAALVSGNG
                     RVAMQSRPQMTPRLRPVR"
     gene            4328..>4509
                     /locus_tag="VIF63_03045"
     CDS             4328..>4509
                     /locus_tag="VIF63_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238388.1"
                     /translation="MREAGQDQWMGRRGMAKPIDKLGHEVRQLLPIGRVEDTADGHRV
                     AAILASFVVIEVVRDRG"
BASE COUNT          688 a         1598 c         1514 g          709 t
ORIGIN      
        1 tcgccgtctc cgacgacaac cggatcgtgg ccgtcggcga ggaggcccgc gagatgatcg
       61 ggcgcacccc cggcaacatc cacgccatcc ggccgatgaa ggacggcgtg atcgccgact
      121 acgtcatcac cgaggcgatg ctccggttct tcatcaacaa ggccatgagc gggcgcatgc
      181 ggatgcgcct cagcggcccg gccgtgatga tctcggtgcc cgcgggcgtg acctcggtcg
      241 agaagcgcgc cgtcaaggac gcggcactca aggccggggc gcgcgaggcg cacctgatcg
      301 aggagcccct cgctgccgcc atcggcgcca acgtgccgat ctcgggaccg tccgggaaca
      361 tggtcatcga catcggcggc ggcacgagcg aggtcgcggt catcgccctc ggcgggatcg
      421 tcgtctcgac gtcgctgcgc gtcggcggca acaagttcga cgagtcgatc acctcgtaca
      481 tccgcaagaa gtacaacctg atgatcggtg agcgcaccgc cgaggaagtc aagatcacga
      541 tcggcacggc gctcccgctc gagcgcgagc tgtcgatgga ggttcgcggt cgcgacctga
      601 tcgccggcct gccgcgcacc atcccgatca cctcgtccga ggtcatggag gccatcgagc
      661 tgccgctcca gcagctcgtc gcggcggtcc gacacgtcct cgaacagacc ccgcccgagc
      721 tgtcgagcga catcatcgac aagggcatgg tcatgtccgg cggcggcgcg ctgctgcgca
      781 acatcgacaa gctcctcacc caggtcacgg gcgtgccctg ccacgtcgcg gagaacgccc
      841 tcaactgcgt cgccctcggc accggcgagg cgctgaagca ctacgacttc ttcaagaagt
      901 cgctcgtcca gaccgtctag acgagttggc gcgcttggca gcgagcgccg ggacggatcc
      961 gaacgccgcc cgcgcgttcg tcgacctgca ctgccacacc tcggccagct tcgactccct
     1021 cgcgtcgccg gctgccgtcg tgcgcgcggc tgcggcccgc ggcctgaccc acctcgcgat
     1081 caccgaccat gaccggatcg acggcgcgct cgaggcgaag gccgctgccg cggtcgaggc
     1141 gcccgggctc accgtgcttg tcggccagga gatccgcacc acggccggcg acctgatcgg
     1201 gctgttcctg cacgagccga tccggcccgg gctggcgccg gccgaggccg tagccgcggt
     1261 acgggcgcag ggtggcctca tcgggatggc ccatccgttc gaccggtttc ggggctccgt
     1321 cgggcgcggt gcggccgtgg agctcgacga gctggcgcgc acggtcgact ggatcgaggc
     1381 gtggaacgcc cgcctgatcg tcggcgacgg caacgcgcgc gcggcccagc tcgcgacgcg
     1441 cgcgggcatc gccggtgtgg ccgtgtccga tgcacacacg accatggagg tcgggatcgc
     1501 ggcaaccatc ctctccggcg atcctgggac gcctgagggc atgcaggcgg ccctcacggg
     1561 gccgctcgag ctcgtcatgg gccgcgcatc cgcctatgtc cggctgttcg gacccgcggc
     1621 gaagctcgtg cagcgcgcac gaggccgtgg ccgcgttcgc gtcgcggccg acgcagcgtc
     1681 acgccgatga ccgaaccgac gcagccggct ccgccggcag cgcccgctcc gccggcagcg
     1741 ccggccccgc ccgccgagga ggccgaggtc cgggccgagc agctctcgct cgcgagccgg
     1801 ctgcgccagc cgcgcacgat cgtctcgctc gtcctgccga tcgtgctgct cgccctgttc
     1861 gtgaagagcc tgcccgggtt caagctcgag gagctgccgg acaagatcct cggcgcgaac
     1921 ccgttgctga tgctgggcgc cttcgtgatc ttctacatcg gcttcccgct gcgcggcctg
     1981 cgctgggcga tgctcgtgcg ccggaccggg ttcccgctca cggtcaagga cgcgaccgag
     2041 atcatcttca tctcgtggct cgtcaactgc ctcgtgcctg ccaagctcgg cgacgtctac
     2101 cgggcgtacc tcctcaagat caacagcacg gtttcgctct cgcgcacgtt cggcacggtg
     2161 ttcatcgagc gcgtgctcga cctcttcgcg atcgtcgtgc tcggccttgc cgccggcttc
     2221 gtgtcgttcc gcagcggcct gccggccgat gtccagatcg tgttcctgat cggggtggtc
     2281 ttcgtgctgc tgctcgccgg cggcctgctg accatgcgca acttcggccg ccggatcatc
     2341 accgccctgc cgctgcccca ccgggtcatc gagttctacg accgattcga agagggcgtg
     2401 ttcgcctcga tcgggcttcg ggcgctgccc cgcctcgtga tcgtgaccgg gctgatctgg
     2461 gcgaccgagg cgacgcggct cttcctcgtc gtccaggcgc tcgggctgcc gggcgtgcac
     2521 ctgggcatca gcggcgcgtt cttcgtggcg ttgtcggcgt cgctgctgac cgcggtgccg
     2581 ctcacgccgg cggggatcgg cttcgtcgag ggcgggatcg tcgggctgct cacgctcgtg
     2641 tacggggtcg cccagacgga cgcgctcgcc atcaccctcg tcgaccgggc gatcagcgtc
     2701 ctctcgatca tcgtcctcgg ctcgatcgtg tacgccgtgt caccgaagcg ccggggcatg
     2761 ggggtccagt cggccgcacc cgcctgacct gggtcggggg tcctatgacc tccgcggtac
     2821 ccgcgacatc cccggtcccc acctctcggg ccgtcacgac acgggaccgc acagcgtatt
     2881 tgtgcgtaca tcgccatctg cccgggccgt catgaacgaa taggggatat ggcctgaatc
     2941 ggtcgcggcc gaagatccgg gcatgacccg taccaacggc cgagctttca acgagtggct
     3001 ccgcgcccag ctcaaggcca agaagatgtc gcagcgccag ctggcgcagc agtcgggcgt
     3061 cgaccattcg acgatctccc gcctggtgcg cggcgaccgc atgccatcgc tcggcaccgc
     3121 aaccaagctc gctcgcgggc tgcgggagct cggcgagaac gacgacggcc cgcagtacct
     3181 cgggctcatg ggtggcggcg tccagaaccc gaccgcccgt gtcgagtatt cactgcgcgc
     3241 cgacgagtcc ctgagcgagg cacaggtccg ccaggtcatg gagtactacc tcgcggtgcg
     3301 catgcgccgc atcggcggtg ccttcagcaa cgacaacagc catctgcagc gcgtcgagcc
     3361 tcgggacaac aacacccggg cgttcgcgcc gccaaccaac agcccgatcg tgccgcgcgc
     3421 tgcgctcgtc tccggcaacg gccgagtcgc aatgcagtcg cgcccgcaga tgactccgcg
     3481 cctccggccc gttcgctgac ggacggctcc tgacgaactc cactaccgac tgggagtgac
     3541 ctccacgctc tcggtcaacg gggcaagacg aacgccggcg ctcggggcgc cggcgttcgt
     3601 tgtttgtcgg cggccgtcgc cccgcccttg actagcgtcc gggatcggag ccgctcggag
     3661 atcgttcaga cggcggcgga gtatcggttg acgtttcatg acgagaggcc gtccacgctt
     3721 ggggagtcat agcgtcggca acgattgcct cgctcgcggc agaacccagg actcgctcca
     3781 taaggagggg agttcgcaga tcgagctgca tcagtcgggg cgaaggtcca tatctgcgac
     3841 ctcgtcgctc cccgattgga acgagccagc cagaagcagc catcgccgaa agcatggcgc
     3901 gggcccgacc cgacgagagt ccgaggacct ccgcgattcg cgtggttcgg acaggcgcca
     3961 tacgagcccc caagaggatc gctggccact cggggctcgc gtggccctcc cggctcaact
     4021 ccacgaacag cgagccgatg tcgtgcggga gcgcggccaa gacgcggttg cgggcatcca
     4081 agcgatttac acagatgctg gcgaagtact cgagccaggg ggtcgccgag tgctcatctg
     4141 gcgcataggt cggaccgtgc gtctcctgga ggacggcaaa gtacctttcc cgacttgcac
     4201 gaatctccga ctccacgttc agcagctccg gggcgccgat cccacaacgc atgagcacca
     4261 gcgaacccgc gacccgcgct gctcgcccgt tgccattgag ccatggatga atcgagacca
     4321 cgttgaggtg cgtgaggccg gccaggatca gtggatgggt cgccgtggga tggcgaagcc
     4381 aatcgacaag ctgggacatg aggtccggca gctgctgcca atcgggcggg tggaagacac
     4441 cgccgacggt cacagggtcg cggcgatact cgcctcgttc gtcgtgatcg aggtcgtgcg
     4501 tgatcgcgg
//
LOCUS       DATBSW010000108         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1066724, whole genome shotgun sequence.
ACCESSION   DATBSW010000108 DATBSW010000000
VERSION     DATBSW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066724"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VIF63_03050"
     CDS             <1..226
                     /locus_tag="VIF63_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238389.1"
                     /translation="AVPTEVPGVPSEVLQPRSTWPDGAAYDVAARKIAHMFHENFEAY
                     AAGVSKAVRNAGPIRADGVDDVPVTGPGEG"
     gene            complement(233..391)
                     /locus_tag="VIF63_03055"
     CDS             complement(233..391)
                     /locus_tag="VIF63_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238390.1"
                     /translation="MNLNIPVVALSIVGVLIATAGIFIDQSIFLIAAGVGTILFAFVL
                     QEMSKRRA"
     gene            464..1390
                     /gene="mdh"
                     /locus_tag="VIF63_03060"
     CDS             464..1390
                     /gene="mdh"
                     /locus_tag="VIF63_03060"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY8238391.1"
                     /translation="MARNKVTVIGAGNVGATTAQRIAEAGLADVVLVDIVEGLPQGKG
                     LDLAEAAPVVRHDARITGTNDYADTAGSDVIVVTSGLARQPGMSRDDLLAKNAGIVKA
                     VVEAAAAQSPDAVLIVVTNPLDAMCHVAMRASGFPRERVLGMAGVLDSARFRTFIAEE
                     LGVSVADTHAFVLGGHGDTMVPLSRYSTVAGIPITELLSPERVKALEERTANGGAEVV
                     ALLKTGSAFYAPAASTFEMVDAILRDRRRVLPCAVYLQGEYGVDGLFVGVPVVLGAGG
                     MQRVIEITLTDAESAAFKKSAAAVQELVDKLS"
     gene            1438..>1911
                     /locus_tag="VIF63_03065"
     CDS             1438..>1911
                     /locus_tag="VIF63_03065"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY8238392.1"
                     /translation="MPDDFDFAFDPVLRLTDTATVRYETIGLAIVLFVGLLLLAWRAS
                     RMSGLRLDDLVFIVVGAVPGAIFGGRLGYVLDHLDFYRVNPNAVLDAGQGGLSLTLAV
                     PIGVLAGAVIARLLGAPVGRWLQASSPALLFVLAAGKLVGVLGGSGQGLPSDLEWA"
BASE COUNT          277 a          675 c          644 g          315 t
ORIGIN      
        1 cgccgtcccg accgaggtgc ccggcgtccc ttcggaggtc ctccagccac gctcgacgtg
       61 gcccgacggc gccgcgtacg acgtcgccgc gcgcaagatc gcgcacatgt tccacgagaa
      121 cttcgaggcg tacgctgccg gcgtgagcaa ggccgttcgg aatgccggcc cgatccgcgc
      181 cgacggggtc gacgacgtgc cggtgaccgg gcccggcgaa ggctgatcgg cgtcaggccc
      241 ggcgcttgga catctcctga aggacgaaag cgaacagaat cgtcccaacc ccggcggcga
      301 tgaggaaaat gctctgatca atgaagatgc cggcggtggc gatcaggact ccgacgatcg
      361 acagagcgac cacaggaatg ttcaggttca tgttgttctt ccctccggcg cactggattt
      421 gagcgccctc caacgacagg atagataccg aggagagccc tgaatggcgc gaaacaaggt
      481 caccgtcatc ggcgccggga acgtcggcgc caccaccgcc cagcggatcg ccgaggcggg
      541 tctcgccgac gtcgtgctcg tcgatatcgt cgaggggctg ccacagggca agggcctcga
      601 cctggccgag gcagccccgg tcgtacgcca cgacgcacgc atcaccggca cgaacgacta
      661 cgccgatacg gccggctccg acgtgatcgt ggtcacctcg ggcctcgccc gccagccggg
      721 catgagtcgc gacgacctgc tggcgaagaa cgcgggcatc gtgaaggcgg tcgtcgaggc
      781 cgcagccgcg cagtcgcccg atgccgtgct gatcgtcgtc accaacccgc tcgacgcaat
      841 gtgccacgtc gcgatgcgtg cctcgggttt cccgcgggag cgggtgctgg gcatggccgg
      901 cgtgctcgat tcggcgcgct tccgcacgtt catcgccgag gagctcggcg tgtccgtcgc
      961 cgacacccac gcgttcgtgc tcggcgggca cggcgacacg atggtgccgc tgtcgcgcta
     1021 ttccacggtc gcgggcatcc cgatcaccga gctgctctcg ccagagcgcg tcaaggcgct
     1081 cgaggagcgc acggcgaacg gtggcgccga ggtcgtcgcg ctcctgaaga cgggctccgc
     1141 gttctacgcg cccgccgcct cgacgttcga gatggtcgac gcaatcctgc gcgaccgacg
     1201 ccgagtcctg ccctgtgctg tgtacctcca gggcgagtac ggcgtcgacg gcctgttcgt
     1261 gggcgttccg gtcgtcctcg gcgccggcgg gatgcagcgc gtcatcgaga tcaccctgac
     1321 cgacgccgag tcagcagcgt tcaagaagtc cgcggccgcc gtgcaggagc tcgtcgacaa
     1381 gttgtcttga tcggcccccg cggcgtttcc gggattggcg gccactagac tctggccgtg
     1441 cccgacgact tcgatttcgc gttcgacccc gtcctccggc tcaccgacac cgcgaccgtg
     1501 cgctacgaaa cgatcgggct tgcgatcgtc ctcttcgtgg ggctcctgct cctcgcctgg
     1561 cgggcgtcgc ggatgtccgg cctccggctc gatgacctcg tgttcatcgt cgtcggcgcc
     1621 gtcccaggcg ctatcttcgg tggccggctc ggctacgtcc tggaccatct cgatttctat
     1681 cgcgtcaatc cgaacgcggt cctcgacgct ggacagggcg ggctcagcct caccctcgcc
     1741 gtcccgatcg gggtcctggc gggtgcggtc atcgcgcgcc tcctcggcgc gccggtgggc
     1801 cgctggctgc aggcgagctc gcctgccctg ctcttcgtcc tcgccgccgg gaagctcgtg
     1861 ggcgtcctcg gcggatccgg ccagggcctg ccatcggatc tcgaatgggc g
//
LOCUS       DATBSW010000109         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_784644, whole genome shotgun sequence.
ACCESSION   DATBSW010000109 DATBSW010000000
VERSION     DATBSW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784644"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VIF63_03070"
     CDS             <1..213
                     /locus_tag="VIF63_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238393.1"
                     /translation="RAAWLAVPALWPNAQEYYAVIALPIAIEVPLVTLAMATPLTPGI
                     IAVGLFAQGVWNRMQPRKDEPGIGVS"
     gene            236..>1975
                     /locus_tag="VIF63_03075"
     CDS             236..>1975
                     /locus_tag="VIF63_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238394.1"
                     /translation="MSRREAALSAGAVFVVALVIRAWAATQITFPRPEDVAYYVGVAR
                     NLVEGRGLTTDAIWSFGTPPLSFPRDAFEVWLPLPSILAAIPMALFGTTFAAAQVSSI
                     LVGSMVCVLAWRLAADVAASRGMPVGRARTLAIGTGLTAAAYLPLILASVQPDSTMPF
                     AALAIVACLLMTRLSRRLSIEGVREEGIDHFVDRTVRDIEVRSRLRVALGVVLALAAL
                     TRNEAIWLALAFAIVEWSNVRGVYSRLSDRVGAWGINVIPVALVAIITFAPWAFRDWL
                     AFGSPFPGQALTNALSLDGRDIFAWADPPTLARYLAAGPARLLELRWIGIVHNLTQVL
                     LLLGIPLSVVGFIGLPAIWRRRPATSLALAADPLRPLLIFSIITFLVAALVFPVSTTW
                     GTFLHASGAIHVLLVISALLLLDSVIGWIGTRRGWTNPVAWLGPAFAVAGCLLFSAAI
                     LPGDGAGARGTADRYAAIGIVFGATRSDTGAPDPANVAVDLATEPGPVISDFPIWFAE
                     ATRHHTLALPNEGPESILDLARSFDPPARLLVVDADNSGIWPAAILSGAPGSECFVPI
                     ELPNLAAYPGALDD"
BASE COUNT          233 a          773 c          632 g          337 t
ORIGIN      
        1 cgcgccgcct ggctcgcggt ccctgcccta tggccgaatg cgcaggagta ctacgcggtg
       61 atcgcgctgc ccatcgcaat cgaggtacca ctggtgacct tggcgatggc gacaccgctg
      121 acgcccggga tcatcgccgt cgggctgttc gcacaggggg tctggaatcg gatgcagcca
      181 cgcaaggacg agccgggtat cggcgtctcg tgaaccgccg cgaggcgatc ctctcgtgag
      241 ccgccgcgag gctgccctct cggccggcgc cgtgttcgtc gtcgccctcg tcatccgtgc
      301 ctgggccgcg acgcagatca ccttcccgcg gcccgaggac gtcgcgtact acgtcggcgt
      361 ggcgcgcaac ctcgtcgaag ggcgcggcct caccaccgac gcaatctgga gcttcgggac
      421 gccgccgctc tcgttcccgc gcgacgcgtt cgaggtttgg ctgcccctgc cgtcgatcct
      481 cgcggcgatc ccgatggcgc tgttcgggac gacgttcgcg gcggcgcagg tgtcgtcgat
      541 cctcgtcggg tcgatggtct gcgtcctcgc ctggcgcctc gccgctgacg tggctgcttc
      601 acggggcatg ccggtcggtc gggcgcggac gctcgccatc gggacgggcc tgaccgccgc
      661 cgcgtacctg cccctgatcc tggcctcggt ccaaccagac tcgaccatgc cgttcgccgc
      721 cctcgcgatc gtcgcgtgcc tgctgatgac ccgcctgagc cggcggctga gcatcgaggg
      781 cgtccgcgag gaaggcatcg accacttcgt cgaccggacc gttcgcgaca tcgaagtcag
      841 gtcgcggctg cgcgtcgcgc tcggcgttgt gttggcgctt gcggccctga cccgaaacga
      901 agcgatctgg ctcgccctcg cgttcgccat cgtcgagtgg tcgaacgtcc gtggggtcta
      961 ctcgcgcctg tccgaccggg tcggcgcctg gggcatcaac gtgatcccgg tcgcgctcgt
     1021 cgcgatcatc accttcgccc cgtgggcgtt ccgcgactgg ctggcattcg gcagcccgtt
     1081 cccgggccag gcgctcacga acgcgctgag cctcgacggc cgcgacatct tcgcctgggc
     1141 cgatccgccg accctcgccc gctacctggc cgccggcccc gcccggctgc tcgagctgcg
     1201 ctggatcggg atcgtccaca acctgaccca ggtgctgctg ctcctcggca tcccgctgtc
     1261 ggtcgtcggc ttcatcggtt tgcccgcgat ctggcgccgc cgacccgcca cctccctcgc
     1321 gctcgccgcc gacccgctcc gaccgctgct gatcttctcg atcatcacct tcctcgtcgc
     1381 ggcgctcgtc ttccccgtct cgacaacgtg gggcaccttc ctccacgcct cgggcgcgat
     1441 ccacgtgctg ctggtgatct cggcgctcct gctcctcgac tcggtcatcg gctggatcgg
     1501 cacgcgccgc ggctggacga acccggtggc ctggctcggt cccgcgttcg ccgtcgccgg
     1561 ctgcctgctg ttcagcgccg cgatcctgcc cggtgacggc gccggcgccc gcgggacggc
     1621 cgatcgatat gcagcaattg gcatagtttt cggcgccacg cgaagcgaca ccggcgcgcc
     1681 cgacccagcc aacgtcgccg tcgacctggc caccgagccg ggccccgtca tcagcgactt
     1741 cccgatctgg ttcgccgagg cgacccgcca ccacacgctc gcgctgccca acgaaggacc
     1801 cgagagcatc ctcgacctgg cccgcagctt cgacccgccg gcgcgcctgc tggtcgtcga
     1861 cgccgacaac tccggcatct ggcccgccgc gatcctgagc ggtgcccccg gcagcgaatg
     1921 cttcgtcccg atcgagctgc ccaacctcgc cgcctacccc ggcgccctcg acgac
//
LOCUS       DATBSW010000110         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_44241, whole genome shotgun sequence.
ACCESSION   DATBSW010000110 DATBSW010000000
VERSION     DATBSW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44241"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VIF63_03080"
     CDS             complement(<1..1133)
                     /locus_tag="VIF63_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8238395.1"
                     /translation="MNPAGHSIHPVIGHEYVRLVSGKGVFVTDDTGKSYLDAVAGIGV
                     VALGYGRDDLVDAYAEQARRLPFAQSMRFENLAAERLAERLAAFAPPGLTWSFFCNGG
                     SEALDSAVKLVRQYWLDRGQSSKWKVIGRRPGFHGNTLMALSVGYHAGRRARYLPYIT
                     DLPHLRAPWIYRCEHGDDMAGPYCDDCSGRALAALIEAEGPETVAAFIAEPIVGAAAP
                     AVTPPAGYYQTIREICDRYDVLFISDEVMTGIGRTGRNFGIEHWDVVPDLMLFAKGIS
                     AGYAPLAGVLAHERVIDVLRSGGGRYENTFTWGGNPMACAVGAATLEAMVREEIVPGV
                     ADVAPAFFGQLEQLRRHPIVGDIRGKGLMAGIEFVQPGTRTPFP"
     gene            1181..1657
                     /locus_tag="VIF63_03085"
     CDS             1181..1657
                     /locus_tag="VIF63_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8238396.1"
                     /translation="MYLNALEFLEEERDAWRPYEALEGLTDEQLDQPIDAAHDWSGRD
                     LIGHLVAWQMNALDVARELAVNEKSATKEQSDRDWDARGDDINTEIQAEWRALPMAEV
                     RRRLREVPGELRGYLTVVPETRWVKHSDHLRFFTEETIEHYADHTADLEAILDSAR"
     gene            1671..1976
                     /locus_tag="VIF63_03090"
     CDS             1671..1976
                     /locus_tag="VIF63_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238397.1"
                     /translation="MIPSLPVLVAEVVVDLPKARSLSVDGVITWSVGDQPFAALGAAG
                     IEIRLDPPIAAAATRTPDTGPSPRGPEWVRFNPHEMDGHAVDRVRAWLELAYRRAGG"
     gene            2149..>2831
                     /locus_tag="VIF63_03095"
     CDS             2149..>2831
                     /locus_tag="VIF63_03095"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238398.1"
                     /translation="MALVGLFGTGPVGPKPAQAGIVINPCLVVECATLTIVMHGDGTA
                     VLNSMKATNKSQIDGIIECHRAGGTTYGSCSHDYVIANPPTHVYLRVHAFPGSGACDS
                     AGQNCAAERTYDVTMDNDFAQHANIVMVDPRTLTVTTSGAGKGAVTSAPVGIDCGSGG
                     SACSMHVAHGATVKLTAATEPGSTFKGWKGACSGSLATCSVKMADDRSVEAVFAKVAT
                     PPPATPKPTA"
BASE COUNT          470 a          984 c          980 g          397 t
ORIGIN      
        1 gggaacggcg tccgggtgcc cggctggacg aactcgatcc cggccatgag gcccttgccc
       61 cggatgtcgc cgacgatcgg atggcggcgg agctgctcga gctggccgaa gaaggccggc
      121 gccacgtctg cgacgccggg cacgatctcc tcgcgaacca tggcctcgag cgttgccgcg
      181 ccgacggcgc aggccatcgg gttcccgccc caggtgaacg tgttctcgta gcggccgccg
      241 ccggaacgaa ggacgtcgat gacccgttcg tgggccagaa cgccggcgag cggcgcgtac
      301 ccggccgaga tgcccttcgc gaacagcatg agatccggga ccacgtccca gtgctcgatc
      361 ccgaagttgc ggccggtccg accgatcccg gtcatcacct cgtcgctgat gaacagcacg
      421 tcgtatcgat cgcagatctc gcggatggtc tggtagtacc cggcgggcgg cgtcaccgcg
      481 ggcgcggcgg cgccgacgat gggctccgcg atgaacgcag cgacggtctc agggccctcc
      541 gcctcgatca gcgccgccaa cgcccggccg gagcagtcgt cgcagtacgg cccggccatg
      601 tcgtcgccgt gctcgcagcg gtagatccac ggcgcgcgga ggtggggcag gtccgtgatg
      661 tacggcaggt atcgagcgcg ccggccggcg tgatacccga ccgacagggc catcagcgtg
      721 ttgccatgga agccgggtcg ccggccgatg accttccact tcgacgattg gccgcgatcg
      781 agccagtact ggcggacgag cttgaccgcc gagtcgaggg cctccgagcc gccgttgcag
      841 aaaaacgacc aggtcaggcc gggcggggcg aacgccgcga gccgttcggc aaggcgttcc
      901 gcggcaaggt tctcgaagcg catcgactgg gcgaacggga ggcgccgcgc ctgctcggca
      961 taggcgtcga cgaggtcgtc gcgcccgtag ccgagggcca ccacgccgat ccccgcaaca
     1021 gcgtcgaggt acgacttgcc cgtgtcgtcc gtgacgaaga cgcccttgcc ggagacgaga
     1081 cgaacgtact cgtggccgat gaccgggtgg atcgagtggc cggccgggtt catggcgggc
     1141 acggtagcgg ccgggcacgt gccccgtcta cgatcacggc atgtacctca acgccctgga
     1201 gttcctcgag gaagagcgcg acgcgtggcg cccgtacgaa gcgctcgagg gcttgaccga
     1261 tgagcagctc gaccagccga tcgacgccgc ccacgactgg tccgggcgcg acctcatcgg
     1321 ccatctcgtc gcctggcaga tgaacgccct cgacgttgcc cgcgagctcg ccgtcaacga
     1381 gaagagcgcg accaaggagc aatccgatcg cgactgggac gctcgcggcg acgacatcaa
     1441 caccgagatc caggccgagt ggcgggcgct gccgatggcc gaggttcgcc gacgcctgcg
     1501 cgaggttccg ggcgagctgc gcggctacct gacggtcgtg cccgagaccc gctgggtcaa
     1561 gcacagcgac caccttcgct tcttcaccga agagaccatc gagcactacg ccgaccacac
     1621 cgccgacctc gaggcgatcc tcgactcggc tcgctgagac acacgccggc gtgatcccgt
     1681 cgctgcccgt cctcgtggcg gaggtcgtcg tcgacctgcc gaaggcgcgc agcctctcgg
     1741 tcgacggcgt catcacgtgg tcggtcggtg atcagccgtt cgcggcgctg ggcgccgccg
     1801 gcatcgagat ccgcctcgat ccgccgatcg cggccgcggc gacacgcacg cccgacacgg
     1861 gaccctcgcc gcgcggcccg gagtgggtgc gcttcaaccc gcacgagatg gacggccacg
     1921 ccgtcgaccg cgtacgcgcc tggctcgagc tggcctaccg ccgcgcgggc ggctgaaccg
     1981 aacacgagcg cgcggcgcgg caccggaaag tcttggggag cgttgatgtc acgatgttcc
     2041 gagcgcatga tccctgcgcg ccccgggccg cgatgggcgc aggggggctc catgcggaag
     2101 gtttcgggcc gaccaagcga gttgatgctc gccatcgtcg ccatggcgat ggccctcgtc
     2161 gggctattcg gaaccggccc ggtcgggccg aagccagcgc aggctggcat cgtcatcaac
     2221 ccttgcctcg tcgtggaatg cgccacgctg acgatcgtca tgcacgggga cggcacggcc
     2281 gtcctcaact cgatgaaggc aaccaacaag tcccagatcg acggcatcat cgagtgccat
     2341 cgcgcgggcg gcaccacgta cgggtcctgc tcacatgact atgtgatcgc caaccccccg
     2401 acccacgtct acctccgggt ccacgcgttt cctgggagcg gggcgtgcga tagcgccgga
     2461 cagaattgcg ctgcggagag gacctacgac gtgaccatgg acaacgactt cgcgcaacac
     2521 gcgaatatcg tcatggtcga tccgcgaacc ttgacggtca cgacctcggg ggcgggcaag
     2581 ggtgccgtga cgagcgctcc cgtcggcatc gactgcgggt ctggcgggtc tgcctgcagc
     2641 atgcacgttg cccacggcgc gaccgtcaag ctgacggctg ccaccgagcc cgggtcgacc
     2701 ttcaaaggct ggaaaggcgc atgcagcggg tcgctggcga cctgctcggt caagatggcc
     2761 gacgaccgct cggtcgaggc ggtcttcgcg aaggttgcga cgccaccgcc ggccacgccg
     2821 aaacccacgg c
//
LOCUS       DATBSW010000111         4794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_397028, whole genome shotgun sequence.
ACCESSION   DATBSW010000111 DATBSW010000000
VERSION     DATBSW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397028"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VIF63_03100"
     CDS             complement(<1..346)
                     /locus_tag="VIF63_03100"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEY8238399.1"
                     /translation="MSRVAILFTGGTISMVADAAAGGKVPTLDGAAILARAPGIGDIA
                     DLVTVDLGRTPASHFTFPKLFEIGSEIRRWQADPSIDGVVLVQGTDVIEETAFLWDLV
                     LDGDAPVIVTGAM"
     gene            complement(343..1926)
                     /locus_tag="VIF63_03105"
     CDS             complement(343..1926)
                     /locus_tag="VIF63_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238400.1"
                     /translation="MTGPGGTTGPSGADGAEVALVPPPESAAAVLRNRPFLLLWLAQV
                     ATQIGINMVLYALEVVVLSATDLSSAVSLLFLTFLVPSVLFSALAGVYVDRIDRRIVL
                     VVTNALRAAILVAIFAAGNDIGLVLLLNTILSTVTVFFAPAEAAMIPVLVPREQLVAA
                     NGIFTLTLNGAFAVGFAFLGPLIVTILGSPEPLLIVVAMLFAVAAVFCITLPADPPVT
                     AEGRSGSLSATAIGATAIGAVSEAGHAVTGVFGQLREGVAYIRANPAIAWSLAYLGIS
                     ASLIGVVAVMGPSFARDTLGLSPEDLIVVVLPLGVGIVMGVLLLNNYGKYIARRRAIE
                     FGLVLLGFLLLILTVAGPLSRILTGVGDQLPLDVSALTSLVAVVVALAFVAGICYGIV
                     AISAQVQLQEDIPADVRGRVFGVLNMLISVASILPIIVVGPISDLIGTTPVILLVAAF
                     VLISGLFSILTRGPLRADEGPDLIPEMVSGTLIDPTGGNVLRDRREPHGHSHSEAHRH
                     GEAHRDRAEAAGSEEPPKP"
     gene            complement(1923..2171)
                     /locus_tag="VIF63_03110"
     CDS             complement(1923..2171)
                     /locus_tag="VIF63_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEY8238401.1"
                     /translation="MSARPVLELFLGDVVRLRRAHPCGGSDWRIDRLGADIGLRCLGC
                     DRHVLLDRRTLEVRLAGFVERGNPPMSAAVTPRPAPEA"
     gene            complement(2225..2905)
                     /locus_tag="VIF63_03115"
     CDS             complement(2225..2905)
                     /locus_tag="VIF63_03115"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY8238402.1"
                     /translation="MTAGRVSSRLSVAVVAALLGILAIGQLRGQAGVPGLSALSATEL
                     TQLIANLSAGNDRLRLEVAELGRQETHLTETRDRGETTVDELTADLDRIRAWAGLTAV
                     TGPGVAITVSGAIGGDGIEELLNELRNAGAEAIVVDGVRVVAGTVVAGPAGGLSIENA
                     AIGDAFEIRAIGSPQILTGTLTRTGGVIAQVGATYPDVRLTVTPVEAMTLPPTERDLG
                     PEHAEPSL"
     gene            complement(2902..3636)
                     /locus_tag="VIF63_03120"
     CDS             complement(2902..3636)
                     /locus_tag="VIF63_03120"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY8238403.1"
                     /translation="MSSLIGRLRGLPSVQVTLAAALLVLGFLIAAQIAAEGPRIRYST
                     EERSPLIETALSLQARQESLKAEILALRSRIGDLEAQDPSAADSLRRLYAALEQARLE
                     AGLVAVSGRGLAFRIEDGSGSLDALVTAHDVRVLVEELWLAGAEAIEVNGERVVGTTA
                     VLDIGGSILVNSAYLAPPYTIRAIGPDDLYDRLRASVAFVEFVQGRIEPSGLHLSVAE
                     LDKVDIEAFAGTVSLRNARPVEGATP"
     gene            complement(3633..>4794)
                     /locus_tag="VIF63_03125"
     CDS             complement(3633..>4794)
                     /locus_tag="VIF63_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEY8238404.1"
                     /translation="TNPTPNGALFPNTRVELGFRVTLADGRSFDGPTTTVLYEDDRFT
                     WRSVEGEVVRVHWYEGDDAFGRRALEIGEKAIENATKLLGVVEEDPIDFYIYTQTSDF
                     RDVIGPGLQENVGGLALAPIRTLFANIAPSSVFDPWVSIVVPHELTHIVFDTATANRY
                     HEPTHWLNEGLADYLAIGYDSAARNNVERAVRSNDLMPLRALVAQFPSTPTRFSLAYD
                     ESVSAIDFLVRTYGKEKLVALIRSYTEGVSDDAAFSAALGVDTDGFEAAWLADLGMEA
                     PVPFGPLPAPAGSLPPGWAQGAVQTPKPGVTSPATTPRPSDPGDTGDVVGPVLLGILV
                     AFGLVIVAGLFVTVRRLNRGEPFMPPLVQAPPSADEPAADDAAPAPDEKDRP"
BASE COUNT          744 a         1654 c         1754 g          642 t
ORIGIN      
        1 gcatcgcgcc ggtgacgatg accggcgcgt cgccgtcgag cacgaggtcc cacaggaatg
       61 ccgtctcctc gatcacatcg gtgccctgga cgaggaccac gccgtcgatc gatgggtcgg
      121 cctgccagcg ccgaatctcg ctcccgatct cgaacagctt cggaaacgtg aagtggctcg
      181 cgggcgttcg cccgaggtcg accgtgacga ggtcggcgat gtcgccgatc cccggcgcgc
      241 gggcgagaat cgcggcgccg tcgagtgtcg ggacctttcc cccggccgcc gcgtccgcca
      301 ccatgctgat cgtcccgccg gtgaacagga tcgcgacgcg gctcacggct tgggcggctc
      361 ctcggacccc gcggcctccg cccggtcccg gtgcgcctcg ccgtgccggt gcgcctcgct
      421 gtggctgtgg ccgtggggct cacgccgatc gcgcagcacg ttcccgccgg tcggatcgat
      481 cagcgtgccc gagaccatct cgggaatgag gtcggggccc tcgtctgccc gcagcgggcc
      541 acgagtcagg atcgagaaca gtccggagat gagcacgaac gccgcgacca ggaggatgac
      601 cggcgtcgtg ccgatcaggt ccgagatcgg cccgaccacg atgatcggca ggatgctcgc
      661 gaccgagatg agcatgttga gaaccccgaa cacccggccc cggacgtcgg ccgggatgtc
      721 ctcctgcagc tggacctgtg ccgagatggc cacgatcccg tagcagatgc ccgcgacgaa
      781 ggcgagggcg acgacgacgg caacgagcga ggtcagggcg gacacgtcga ggggcagctg
      841 gtcgccgacg ccggtcagga tccggctgag tggcccagca accgtcagga tcagcagcag
      901 gaagccgagc agcacgagcc cgaactcgat cgcccgccgc cgggcgatgt acttgccgta
      961 gttgttgagg agcaggacgc ccatcacgat gccgacgccg agcggcagca cgacgacgat
     1021 gaggtcctcc gggctcagcc cgagcgtgtc ccgagcgaac gacgggccca tgaccgcgac
     1081 cacgccgatg agcgacgcgg agatcccgag gtacgcgagc gaccaggcga tcgcgggatt
     1141 ggcccggatg taggcgacgc cctcgcggag ctggccgaac acgcccgtga ccgcgtggcc
     1201 tgcctcgctc accgcgccga tcgccgtggc gccgatggcg gtcgcgctga gcgagccgga
     1261 ccggccctcg gccgtgacgg gtgggtccgc cggcagcgtg atgcagaaca ccgccgcgac
     1321 ggcgaacagc atggcgacga cgatgagcag cggctcgggc gagccgagga tcgtcacgat
     1381 gagcggcccg aggaacgcga acccgaccgc gaacgcgccg ttcagggtca gcgtgaagat
     1441 gccgttcgcg gcgacgagct gctcgcgcgg cacgaggacg ggaatcatcg ccgcctcggc
     1501 cggcgcgaag aacacggtca ccgtcgacag gatcgtgttc agcagcagca cgagcccgat
     1561 gtcgttcccg gcagcgaaga tcgcgacgag gatcgccgct cgcagcgcgt tcgtgaccac
     1621 gagcacgatc cgccggtcga tccggtcgac gtacaccccg gcgagcgccg agaacagcac
     1681 ggacggcacg aggaacgtga ggaacagcaa gctgaccgcg gagctcaggt cggttgcgct
     1741 gaggacgacg acctcgagcg cgtacaggac catgttgatc ccgatctggg tcgccacctg
     1801 ggcgagccac aggagcagga acggtcggtt ccggaggacc gcggctgctg attcgggtgg
     1861 cgggacgagc gcgacctcgg cgccgtccgc gccgctcggg ccggtggtgc cgccggggcc
     1921 ggtcacgcct ccggggccgg ccgaggcgtg accgcggcgc tcatcggtgg attgccgcgc
     1981 tccacgaagc ccgcgaggcg gacctcgagc gtgcgtcgat ccagcaggac gtgccggtcg
     2041 caaccgaggc agcggagccc gatatcggcg ccgaggcggt cgattcgcca gtcgctgccg
     2101 ccacacggat gggcgcggcg gagccggacg acatcgccca ggaacagctc gaggacgggc
     2161 cgggcggaca tcggcgggta ccttaccaga ggcagccgga ctggccgggc gaccgcgcgc
     2221 gagatcaaag cgacggttcg gcgtgctccg gcccgaggtc gcgttcggtg ggcgggaggg
     2281 tcatcgcctc gaccggggtg acggtcaggc gcacgtccgg ataggtcgcc ccgacctggg
     2341 cgatcacgcc gccggtccgg gtcagcgtgc cggtcaggat ctgggggctg ccgatcgccc
     2401 gaatctcgaa ggcgtcgccg atggcggcgt tctcgatcga caggccgccg gccggacccg
     2461 cgacgaccgt tccggcgacc acccgcacgc catcgaccac gatcgcctcg gcgcccgcat
     2521 tgcggagctc gttgagcagc tcctcgatgc cgtcgccgcc gattgcgccg ctgaccgtga
     2581 tcgcgacgcc cggcccggtg accgcggtga ggccggccca ggcccggatt cggtccaggt
     2641 ccgcggtcag ctcgtcgacg gtcgtctcgc cgcggtcgcg ggtctcggtc aggtgcgtct
     2701 cctggcggcc gagctcggcg acctcgaggc gcagccggtc attgcccgca gacaggttgg
     2761 cgatgagctg cgtcagctcg gtcgcggaca gggccgacag gcccggcacg cccgcctgcc
     2821 cccgcagctg cccgatggca aggatgccga gcagcgccgc cacgacggca acggacaggc
     2881 ggctgctcac gcggcctgcg gtcatggcgt ggcgccctcg actggtcggg cattgcgcag
     2941 gctcaccgtg ccggcgaacg cctcgatgtc caccttgtcc agctcggcga ccgacaggtg
     3001 gagccccgac ggctcgatgc gcccctggac gaactcgacg aacgcgacgg aggcgcgcag
     3061 gcggtcgtac aggtcgtcgg gcccgatggc cctgatcgtg tacggcgggg cgagatacgc
     3121 cgagttgacg aggatcgagc cgccgatgtc gaggacggcg gtcgtgccga cgacgcgctc
     3181 gccgttcacc tcgatggcct cggcacccgc aagccacagc tcctcgacga ggacccgcac
     3241 gtcatgcgcg gtcacgaggg cgtcgaggct gccgctcccg tcctcgatcc ggaaggcgag
     3301 accgcggccc gacacggcga cgaggcccgc ctcgagccgc gcctgctcga gggcggcata
     3361 caggcggcgc agcgaatcgg ccgcgctcgg gtcctgcgcc tcgaggtcgc cgatgcggct
     3421 gcgcagcgcc aggatctcgg ccttcaggga ctcctgccgg gcctggagcg agagtgcggt
     3481 ctcgatcagc ggcgagcgct cctcggtcga gtagcggatt cgcgggcctt ccgccgcgat
     3541 ctgggcggcg atcaggaatc cgaggacgag gagcgcggcg gcgagcgtga cctgcacgct
     3601 gggcaacccg cgcaggcgac cgatgagcga gctcacggcc ggtctttctc gtcaggtgcc
     3661 ggcgcggcat cgtcggctgc cggctcgtcg gcggatggcg gtgcctggac gagtggcggc
     3721 atgaacggct cgccccggtt gagccgccgg accgttacga acagcccggc cacgatcacg
     3781 aggccgaacg cgaccaggat gccgagcagg actggcccga cgacatcgcc ggtatcaccc
     3841 ggatcggacg gtcggggcgt cgtcgctggg ctggtgacgc ccggcttcgg ggtctgcacg
     3901 gcgccctggg cccagcccgg tggcagcgag cccgccggcg ccggcagcgg cccgaacggt
     3961 accggcgcct ccatccccag gtccgcgagc cacgccgcct cgaacccgtc ggtgtcgacc
     4021 ccgagggccg cgctgaacgc agcgtcgtcg ctcacgcctt cggtatagct ccggatcagc
     4081 gcgacgagct tctccttgcc gtacgtccgc acgaggaagt cgatcgccga gacactctcg
     4141 tcgtaggcga ggctgaatcg cgtgggggtc gaggggaact gggcaaccag cgcccgcagg
     4201 ggcatcaggt cgttcgagcg aacggctcgc tcgacgttgt tgcgggccgc gctgtcgtag
     4261 ccgatcgcga ggtagtcggc gaggccctcg ttcagccagt gcgtcggctc gtggtagcga
     4321 ttggcggtgg cggtgtcgaa cacgatgtgc gtcagctcgt ggggcacgac gatgctgacc
     4381 caggggtcga agaccgacga cggagcgatg ttcgcgaaca gcgtccgaat cggtgcgagc
     4441 gcgaggccgc cgacgttctc ctggaggccc ggtccgatga cgtcccggaa gtcgctcgtc
     4501 tgcgtataga tgtagaagtc gatcgggtcc tcttccacga cgccgagcag cttcgtcgcg
     4561 ttctcgatcg ccttctcgcc gatctcgagc gcccggcgtc cgaaggcgtc gtcgccctca
     4621 taccagtgca cgcgaacgac ctcgccctcg acggaccgcc aggtgaagcg atcgtcctcg
     4681 tacagcacgg ttgtcgtcgg gccgtcgaac gatcggccgt cggcgagggt gacccggaag
     4741 ccgagctcga cccgtgtgtt cgggaacagc gcgccgttcg gcgtcgggtt cgtg
//
LOCUS       DATBSW010000112        13820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_432523, whole genome shotgun sequence.
ACCESSION   DATBSW010000112 DATBSW010000000
VERSION     DATBSW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13820
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432523"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..746)
                     /locus_tag="VIF63_03130"
     CDS             complement(132..746)
                     /locus_tag="VIF63_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238405.1"
                     /translation="MVRLWSEIRGRRAKELLADVATLAWLALWGAIAWNIYQAVAGFA
                     DAGRTIRGAGETMIQGGIDLGAALAGVPLIGTGLRDVAEHAFAGAGTPISTFGTDLQG
                     LILLIAALLGLLILLVPTIPWLNRYVPWRWARLRSMRAGHRAIRIAPVADSVAVREVL
                     ALRAVTRLDYRDLLAFTPDPIGDWVAGRHDRLAQAELASVGLRA"
     gene            1409..3316
                     /locus_tag="VIF63_03135"
     CDS             1409..3316
                     /locus_tag="VIF63_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8238406.1"
                     /translation="MGAVSKAAEIGLSPRTSTGVSSWADPSGIRGVIRHTLGRQAAMI
                     LLGASLVGAISGVGNPLVFIALAAGALGQFVVPPALRRLGRPLTPETDFRIAVAGWPI
                     ALVVLSSVGWAPDKDLGEVIAVAGFMMAALVALAESTIVSVGWTVLAAAGVTVGASIG
                     GHLTVETLIAAASIAGGTFLGARLRSAIEGFLGARRRLMRDVARVPVERDPFLTADFL
                     LSALARWTPLRTPSIIWFTPDGRSTFLAISGDNLPPDLAAGRDLPASRNGLLRSLSQN
                     GPWISGWTVQSDDGGYSRRIAATGVQAAAYVPLVFEGQVIGVVAAGLTDRGDDRSAMA
                     EFVPTLVEFADAAGVALGPSLAEKVQVSTARQAIESILERGAFTPVFQPVVRLGDGRI
                     VGYEALTRFVSELNTGEIFARARVAGQLRELEVATMAAAAVAAATLPPDRWLSLNCSP
                     DLLIDTEVLAGLLAPIRQPVVLELSEQDRITDYGPIAAALERIGPKVRLAVDDAGAGF
                     ASLRHILEVRPQFVKLDIGLVQGVATDLTRTALVAGFVRFAADAAFELIAEGIETDAD
                     RQALRRLGVTFGQGFLLGRPGPAAEVSGPAVRRARSTRSPRLRFRRDDNPDREPALPT
                     PRTGGSAIPLR"
     gene            3243..4592
                     /locus_tag="VIF63_03140"
     CDS             3243..4592
                     /locus_tag="VIF63_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEY8238407.1"
                     /translation="MTTTPIGSPRSQRRAPADLPFRFADKQLAEILAEERGDPDWLSA
                     DRRSAWEAFEALPTETNQLYTPYIDLRSASLDDVRPYVLDGPSGLGDDLRALRAASPV
                     PGVIVETFSEWLARDPDGYRAALEGGATLPAGDKLAMFARGFWSHGHHVDVAKGARVA
                     APIVISWPSAAPGRALVSRTIITVGEGASASIVEELVAAGPDIDCAEGETVPQGFFAG
                     TTEVVLGAGATLSIASIQDLPADQVVFLHRHAAIGEGASLHWALAQLGGRLVRSRVDN
                     RLEGDRSSVEQVEIVFGNEQQLFDLTSYTRHIGRDTTGNLLSKGALLDGARSYMKGLI
                     TIEKSAVGTDSFLGEFGMNLSKGARAVAIPSLEIDQPDCRRAMHSSSVGPIDPNQLFY
                     LESRGISPDEARKFIVLGFLEPVVARVPLEATRDRLRELLEDKWAAGLGAGAAGAAA"
     gene            4589..4960
                     /locus_tag="VIF63_03145"
     CDS             4589..4960
                     /locus_tag="VIF63_03145"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8238408.1"
                     /translation="MTRRVDLLALDEVPEGTMKMAWVDGTDQVIVVHTGGEIRAFQGI
                     CTHEYFELDKGFLTGGGSVGANGVPAGTLTCALHLSRFDLADGEALDPPAEVPLAAYP
                     VAIEDGRILIEIPDGSLPVNE"
     gene            complement(4961..7612)
                     /locus_tag="VIF63_03150"
     CDS             complement(4961..7612)
                     /locus_tag="VIF63_03150"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY8238409.1"
                     /translation="MIRWTFLLVTLALIGVMVFGAILTQRGWPQTTGSIEIAGLHDTV
                     NVVRDRSGLIQITAGDPHDLFLAQGYVHAQERMWQMEISRRIGAGRLSELFGKTSIDR
                     DKYIRTLGWRVAAERDLAAMSPEAKAVLQAYSDGVNAWISEHDGKLSTSFVVAGLLSG
                     TGGIGGIKLEPWTPLDVATWQKVQAWSLGGNANTEIFRLLADARLGDPAKTDELFPAY
                     RPDAPVITPTGLEGSGGAGAAGAAASAGSGATAAGPSVDLTAEHASALVDLARLGTQI
                     STLAGLDGGDGLVGSHGVGSNNWVVSGDKTLSGKPILANDPHLGFGMPSVWIMNGLHC
                     RTVGDACPWDVVGVSFPGTPAVILGHNARIAWGATNVGPDTEDLFLETADPADATGHY
                     IYKGTSQPYQVRHETIKVAGGADVEIDVRSTIHGVVLSDVDKRLANGPVLALRWTTTA
                     EVDLALESFLKIDTATNFEEFKAAFDTYGSPSQNFVYADVDGHIGYVLPGLIPIRANG
                     CPDPCVGTPVIGGDRVRDGASGNLDWIGYIPREDLPWQLDPAGGQIVSANNAAVDGDY
                     PHWIGRDWDPGYRAARISQLLAEAPAKLTAEDMRSIQMDTYVLRADRVMSGLEALGPT
                     IGTEDGRVVWDRLLKWDRRCDIDSVGCAAYMSVEYALQRAIFDDELGPVAREYVGSVF
                     SWEALIALLDERDSAWWKDVTPNSQRDGNAAAVAGAAIDAAGALLRAAYGDPATWTWG
                     RLHTVTFKEQTLGSSGLAPLELYFNPTARPVAGANGAVDNNYYRISLAYPDPTDLSYK
                     PVGFESLFAVTNGPSYRFTVDMNDLDGAQIIITTGQSGNPFDVHYGDLIPLWANGESI
                     PLPFSPGNVAGSAAQTLDLVKPKPHPP"
     gene            complement(7664..8668)
                     /locus_tag="VIF63_03155"
     CDS             complement(7664..8668)
                     /locus_tag="VIF63_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238410.1"
                     /translation="MALPPLRWFSAADVLAAMPPLEERIALAERTMVALATPGASELP
                     PKIAIHPRPADAFVHAMPAHLRGADASNDLVGMKWVAGYASNIALGMASISAVVILND
                     PDTGVPVAMLDGGPITAERTAAVSGLAIRRWGPAALAGGGRTYIALIGAGVQGRSHLP
                     LLGAVLPGATLHVFDRSPERAEALAAEAHATAGIGETIVHETARAAVESADVIVTAAS
                     FGPKEERQSMTNAWLRPDATVVPIDYATYCAAEVARNAALFIVDQREQFLANRDAGNF
                     DGYPDPGTTIGEALLAGTPRPAGRVVISHLGVGLTDLVFADAIVRAAVAKGLGTTLPR
                     "
     gene            8730..9905
                     /locus_tag="VIF63_03160"
     CDS             8730..9905
                     /locus_tag="VIF63_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY8238411.1"
                     /translation="MPPIDVAQSLENLKLERDAIALYDALARIEKDRRRAEAFRSIAG
                     NERRHAEVWAKKLRALGATVPEPDGPRLRVRTIVLLARLFGTHAVRDLVQALEGDEED
                     AYTAQSTPEVEAIAADEREHAEIWKRLSGTEGASPAFVRPDGLGPGTGHNFTGPRDIA
                     AREGWHRAGRSGTLRAVIFGVSDGLVSNLALVMGIAGASQGEGSFVLLAGIAGLLAGA
                     FSMAAGEYVSMQSQRELFERQIALERAELEAMPEEEQAELASVYRSKGFTREEADTIA
                     KRIFADPKHALDTLVREELGLDPDELGSAWGAAGGSFVAFAIGALIPVVPYLLGAGST
                     AFIASIALSIAALFAVGAGVSLLTGRGLLFSGSRQVFIGAAAAIVTYLVGHVIGVAV"
     gene            10255..11361
                     /gene="lgt"
                     /locus_tag="VIF63_03165"
     CDS             10255..11361
                     /gene="lgt"
                     /locus_tag="VIF63_03165"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8238412.1"
                     /translation="MIDWTPNPVLFQIGGFPLNWYGIAYAVGLGVAYWVMVRQARRLG
                     EDPSIIGNSLVVIAVAALIGGRLYHVIDQWQLYADCPIKIVIPIQFDQGCSGSFHFAG
                     FAGLGVFGGFITGALAFIFLATRWYKVSAWRWADIVAPGLFAMQAIGRWGNFFNQELY
                     GPPTTLPWGIRIDCAHRVVDPAANIDYSCTALPLETTHFHPLFLYESVSALIGLAVIL
                     WLTRRPPSWLRRGDLTPIAVIWIGAARFLIEFLRIGNWRLGEIPTAQIIGAGFVVTGV
                     ALLWLRRRQDAPAFLDGVVPIEDEDREGDGFDDDDDEDLEDDGSDDDDTDLDDDIDGD
                     IDGDEDEPPATADAESPAPPAADAEPLPPSAAPG"
     gene            11424..12233
                     /locus_tag="VIF63_03170"
     CDS             11424..12233
                     /locus_tag="VIF63_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8238413.1"
                     /translation="MAAARGGAREGLDWLGRAPEAKASLLYRAVRLVTRFVLFGLLRF
                     RIETSGQEHLPRGGYLLIAGAHRGWIDPFVAMHAVPAEPRCWFLGSGPSTFTSRWRES
                     LIHRLGGLLPVWRGGLGIDGHVRSARAVVEAGAVFVQMPEGTINGPPGTLGPFRPGAA
                     LIALRTGATIVPLAMAGTEELYLGRRMASRILPSTSVADLLGSSWDGQRPEEGSRAEM
                     DLGRRASDALAARIKPVVEALQPGTVDPPDHPRRLRRRLTWLLLGPGPVEH"
     gene            12230..12754
                     /locus_tag="VIF63_03175"
     CDS             12230..12754
                     /locus_tag="VIF63_03175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY8238414.1"
                     /translation="MNVTIRAEGPGEADAVRAINDAAFGKPSDGAIVDAIRGTDRWIV
                     GGSLVAEDPDGALVGHLLASEGDLDMPDGKIRRIWMVGPVAVIPARQRTGVGSALVQA
                     VIELATERGQPLLCLLGHPSYYPRFGFEPARALGIDPARPWPDEAWMVRRLPAWSPSL
                     RGVARFPDAFPDSQ"
     gene            complement(12765..13154)
                     /locus_tag="VIF63_03180"
     CDS             complement(12765..13154)
                     /locus_tag="VIF63_03180"
                     /inference="COORDINATES: protein motif:HMM:NF018672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEY8238415.1"
                     /translation="MSLIAVEAAALAALIHVWFFVLESVRFMRPNVYARFGIESEEDA
                     KVVRSFAYNQGFYNLFLAAGVAIGIALLATGDVAGGRAIILFACGSMVAAGVVLYLHK
                     PSLLRAAVIQVVPALIAIVVGLVLRPV"
     gene            13214..>13820
                     /locus_tag="VIF63_03185"
     CDS             13214..>13820
                     /locus_tag="VIF63_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8238416.1"
                     /translation="MKYAESILDLVGGTPLVRLTHIPRDLGPLDSQPLILAKLETMNP
                     GGSVKDRIGLPMIEAAERAGLLKPGGTIVEPTSGNTGHGLAIAAALKGYRCIFVMADK
                     QSPEKQALLRAYGAQVVLCPTNVEPESPESYYSVAARLARDIPGAFKPDQYWNQENPT
                     AHEATTGPELWEQTEGRITHFVASVGTGGTITGAARYLRTQK"
BASE COUNT         2018 a         4872 c         4810 g         2120 t
ORIGIN      
        1 cgtgttgcct cgaatccccc gccgccgatc tggtccgatc ggcgtgaccc accagtttcg
       61 ggcgactcga ccgccccgtc atgaaagtcc gtcattggcc cgtaggccgc ccggtcgatg
      121 gaggtcagac ctcaggctcg gagcccgacg ctcgccagct ccgcctgcgc cagtcggtcg
      181 tggcgacccg cgacccagtc gccgatcggg tcgggagtga aggccaggag atcccgatag
      241 tcgaggcgcg tgaccgcgcg cagcgccagg acctcccgga ccgcgaccga atccgccacg
      301 ggcgcgatac ggatcgcccg gtggcccgcc cgcatcgacc ggagcctggc ccagcgccac
      361 ggcacgtacc ggttcagcca cgggatcgtg ggcacgagga ggatcagcag gccgagcagg
      421 gcggcgatca ggaggatcag gccctggaga tccgttccga acgtggaaat gggtgttccc
      481 gcgcccgcga acgcatgctc ggccacgtcg cggagcccag tgccgatcag cggcacgccg
      541 gcgagcgcgg cgccgaggtc gatgccgccc tggatcatcg tctcgccggc gccgcggatc
      601 gtccggccgg catccgcgaa ccccgcgacg gcttggtaga tgttccacgc aatggcgccc
      661 cacagcgcca gccaggcgag cgtggcgacg tccgcgagca gttccttcgc ccggcgtcct
      721 cgaatctcgc tccagagccg aaccatcgcg ttcctccagt cgcccagggg aaccggatcg
      781 gcatcgcgga ttccgcccgg aatcaaaacg cccgagagaa cccgatcact agatgctcct
      841 cgcccataca agtacaggtc cggatcacta gatgttctgg ccctctcgag caaagcgaac
      901 tatagcgggt tggcggccgc ttgtcccaac gattccgcaa cggatttcgc ccgccgcatg
      961 cattcggcat tgcggcttca acgccggagg cgtggtcctc gacctcaccg gcaccttgac
     1021 cctccacggc caggacgacc caagctcgat ctgcccggcg gccaacggcc catgacggac
     1081 tgtcacaacg gggcggtcga gccgcccgaa actggttggt cacgcgatcg gaccagatcg
     1141 gcggcggggg attcgaggca gcacgacatg atcaagacac ggcttcgtgg gcgcgggttg
     1201 cccctgccag ctaccaccct cgccctcgtc ggcctgctcg gatccccgtc gcacatcgag
     1261 cgtcgaggac acgggccggg acctccggta cttccgacgc atggtgcgtg atcgcgccac
     1321 cacatacggt cagtcaacgc ccccatcgga gttcaatcgt ccatgcccgc gcagttctgg
     1381 acacgcacgg ctacccccgg attggacctt gggcgccgtc agtaaggcgg ccgagatcgg
     1441 actctctccg cggacctcca cgggcgtgag ctcatgggcg gatccgtcgg gcattcgcgg
     1501 cgtgatccgc cacaccctcg gtcggcaggc cgcgatgatc ctgttgggcg cctcgctcgt
     1561 cggcgcgatt tccggggtcg gcaatccgct ggtattcatc gccctcgccg cgggcgccct
     1621 ggggcagttc gtcgtcccac cggccctgcg ccgcctcggt cgcccgctca cccccgagac
     1681 cgactttcgc atcgcggtcg ccggctggcc catcgcgctc gtcgtcctca gctctgttgg
     1741 ctgggcgccc gacaaggacc tcggcgaggt catcgcggtc gccggcttca tgatggcggc
     1801 cctcgtggcg ctcgccgaat cgacaatcgt ctccgttggc tggaccgttc tggcggcggc
     1861 gggggtgacc gtcggggcga gcatcggtgg gcatctcacc gtcgaaacgc tcatcgccgc
     1921 ggcatcaatc gcgggcggga ccttcctcgg cgcccgccta cggagcgcaa tcgagggctt
     1981 ccttggcgcc cgccggcgac tgatgcgcga cgtcgcgcgc gtgcccgtgg agcgcgaccc
     2041 gttcctcacc gccgacttcc tgctctcggc cctggcgcgc tggacccccc tgcgtacgcc
     2101 atcgatcatc tggttcacgc ccgatggccg gtcgaccttc ctggcaatct cgggcgacaa
     2161 cctgccgccg gaccttgccg cgggtcgtga cctgccggcg agccgcaacg gcctccttcg
     2221 ttcgctctcg cagaacggac cctggatcag cggctggacc gttcagagcg atgacggggg
     2281 ctacagccgc aggatcgccg caaccggggt gcaggcagcg gcctacgtgc cgttggtgtt
     2341 cgaggggcag gtcatcgggg tcgttgctgc agggttgacc gaccgcggtg acgatcgctc
     2401 ggcgatggcc gagttcgtgc cgaccttggt cgagttcgcg gacgccgccg gggtcgcact
     2461 gggtccgtct cttgcggaaa aggtccaggt ctcgacagct cggcaggcaa tcgagtccat
     2521 cctggaacgc ggtgcgttca cgccggtctt ccaacccgtg gtccggctcg gcgacggacg
     2581 gatcgtgggc tacgaagccc tgacgaggtt cgtgtcggag ctgaacacgg gcgagatctt
     2641 cgcccgggcc agggtcgcgg gccagctgcg cgagctcgaa gtcgccacga tggctgccgc
     2701 ggccgtggcg gccgcgacct tgccgcccga tcgttggctg agcctgaact gctccccgga
     2761 cctgctgatc gacaccgagg ttctggcggg gctcctcgcg ccgatccgcc agccggtcgt
     2821 ccttgagctg tccgagcagg acaggatcac ggactacggg cctatcgctg ccgcgctcga
     2881 gcggatcgga cccaaggtgc gccttgccgt cgacgatgcc ggggcgggct tcgcgagcct
     2941 gcgacacatt ctcgaggtcc ggccgcagtt cgtgaagctg gatattggcc tggtccaggg
     3001 cgtcgcgacc gacctgaccc ggaccgcgct cgtcgccggg ttcgttcgct tcgcggccga
     3061 cgcagccttc gagctgattg ccgaggggat tgaaaccgac gcggatcgac aggcacttcg
     3121 gcgcctcggc gtcacgttcg gtcagggctt cctgctcggc cggcccggcc cggcggccga
     3181 ggtcagcggc ccagccgttc gccgcgcgcg ctctacccga agccctcggc tacgctttcg
     3241 gcgtgacgac aaccccgatc gggagcccgc gctcccaacg ccgcgcaccg gcggatctgc
     3301 cattccgctt cgctgacaaa cagctggctg agatcctcgc cgaagagcgg ggcgatccgg
     3361 actggctcag cgccgatcgg cgctcggcct gggaggcatt cgaggcgctg ccgaccgaga
     3421 cgaaccagct ctacacgccc tacatcgacc ttcgctcggc gtcgctcgac gacgtgcggc
     3481 cgtacgtcct cgacggcccg agcgggctcg gcgacgacct tcgagcgctc cgcgccgcgt
     3541 cgcccgtgcc gggcgtcatc gtcgagacgt tctcggagtg gctggcgcgc gaccccgacg
     3601 gctatcgagc ggcgctcgag ggcggcgcga ccctgccggc cggcgacaag ctcgcgatgt
     3661 tcgcgcgcgg cttctggagc cacggccacc acgtcgacgt cgcaaagggc gcgcgtgtgg
     3721 ccgcgccgat cgtcatcagc tggccatcgg cggcgcccgg tcgcgcgctg gtcagccgca
     3781 cgatcatcac ggttggcgaa ggcgcgtccg cctcgatcgt cgaggagctc gtcgccgccg
     3841 gcccggacat cgactgcgcc gagggcgaga ccgtgcccca gggcttcttc gccggcacga
     3901 ccgaggtcgt gcttggcgcc ggcgcgacgc tgtcgatcgc gtcgatccag gacctgcccg
     3961 ccgaccaggt cgtgttcctg catcgccacg ccgccatcgg tgagggcgct tcgctccatt
     4021 gggcactcgc ccagctgggc ggccggctcg tccgttcgcg ggtcgacaac cggctcgagg
     4081 gcgatcgcag ctcggtcgag caggtcgaga tcgtgttcgg caacgagcag cagctcttcg
     4141 atctgacctc gtacacccgg cacatcgggc gggacaccac cggcaacctg ctctcgaagg
     4201 gcgcgctgct cgacggcgcc cggagctaca tgaagggcct gatcacgatc gagaaatcgg
     4261 cggtcgggac ggactccttc ctcggcgagt tcgggatgaa cctgtcgaag ggcgcgcgcg
     4321 ccgtcgcgat cccgtcgctc gagatcgacc agccggactg ccgtcgcgcg atgcactcgt
     4381 cgtcggtcgg acccattgac ccgaaccagc tcttctacct cgaatcgcgg ggcatctcgc
     4441 ctgacgaggc gcgcaagttc atcgtcctcg ggttcctcga gccggtcgtc gcgcgcgtcc
     4501 cgctcgaggc gacgcgggac cgcctgcgcg agctgctcga ggacaagtgg gcggccggcc
     4561 tgggcgcagg cgcggccggc gcggcggcgt gacccggaga gtcgacctgc ttgccctcga
     4621 cgaggtgccc gagggcacga tgaagatggc ctgggtcgat ggcacggacc aggtcatcgt
     4681 ggttcatacc gggggcgaga tccgcgcctt ccagggcatc tgcacccacg agtacttcga
     4741 gctcgacaag gggttcctca cgggcggcgg ctctgtcggg gcgaacggcg tgcccgccgg
     4801 caccctgacc tgcgcgctgc acctgtcgcg cttcgacctc gccgacggcg aggcgctcga
     4861 cccgcccgcc gaggtgccgc tggccgccta tccggtggcc atcgaggacg gccgcatcct
     4921 catcgagatc cccgacgggt cgctccccgt caacgagtga tcagggcggg tgcggcttcg
     4981 gcttcaccag gtccagcgtc tgggcggcgc tccccgcgac gttgccagga gagaatggca
     5041 aggggatcga ttcgccgttc gcccagagcg ggatcaggtc gccgtagtgc acgtcgaagg
     5101 gattgccgct ctggccggtc gtgatgatga tctgggcgcc gtcgaggtcg ttcatgtcga
     5161 ccgtgaaccg gtatgacggc ccgttcgtga ccgcgaacag cgactcgaag ccaaccggct
     5221 tgtagctcag atcggtggga tccgggtacg cgagcgagat tcggtagtag ttgttgtcga
     5281 cggcgccatt cgcgcccgcg acgggccggg cggtcgggtt gaagtacagc tcgagcggcg
     5341 cgaggccgga cgagccgagc gtctgctcct tgaacgtgac cgtgtgcaac cggccccacg
     5401 tccaggtggc cggatcgcca tacgccgccc gaagcagcgc gcccgccgcg tcgatcgccg
     5461 cgccggcgac ggcggccgcg ttgccgtcgc gctggctgtt cggcgtgacg tccttccacc
     5521 acgcgctgtc tcgctcgtcg aggagcgcga tgagcgcctc ccacgagaac accgacccga
     5581 cgtactcgcg cgcaaccggc ccgagctcgt cgtcgaagat cgcgcgctgg agcgcgtact
     5641 cgacgctcat gtatgccgcg caaccgacgg aatcgatgtc gcaccgccgg tcccacttga
     5701 gaaggcgatc ccacacgacg cgcccgtcct cggtcccgat ggtgggtccg agcgcctcga
     5761 gaccgctcat cacgcggtcg gcccggagca cgtaggtatc catctggatc gagcgcatgt
     5821 cctcggcggt gagcttcgcg ggtgcctcgg ccaggagctg gctgatgcgc gccgcacggt
     5881 agccggggtc ccagtcgcgt ccgatccagt gcgggtagtc cccgtcgacc gcggcgttgt
     5941 tcgcggagac gatctgcccg cccgccggat cgagctgcca gggaaggtcc tcgcgcggga
     6001 tgtagccaat ccagtcgagg ttgcccgaag cgccatcgcg cacgcggtcg ccgccgatca
     6061 ccggcgtgcc cacgcacggg tccggacagc cgttggcgcg aatgggaatg aggccgggca
     6121 ggacgtagcc gatgtggccg tcgacgtcgg cgtagacgaa gttctggctc ggcgagccgt
     6181 aggtgtcgaa cgccgccttg aactcctcga agttcgtcgc ggtgtcgatc ttcaggaacg
     6241 actcgagcgc gagatcgacc tcggcagtgg tggtccagcg cagggcgagc accggcccgt
     6301 tggcgagccg cttgtcgacg tcgctcagga cgaccccgtg gatcgtcgac cgcacgtcga
     6361 tctcgacatc ggcgccgccg gcgaccttga tcgtctcgtg gcggacctgg tacggctgcg
     6421 atgtcccctt gtagatgtag tgacccgtcg cgtcggctgg gtcggcggtc tcgaggaaca
     6481 ggtcctccgt gtccggcccg acattcgttg cgccccacgc gatccgcgcg ttgtggccga
     6541 ggatcaccgc cggcgtccca gggaagctca caccaacgac gtcccacgga caggcatcgc
     6601 cgacggtgcg gcaatggagc ccgttcatga tccagaccga aggcatgccg aagccgagat
     6661 gcgggtcgtt ggcgaggatc ggcttgccgc tgagcgtctt gtcgcccgag acgacccagt
     6721 tgttcgagcc gacgccgtgg ctgccgacga gcccgtcgcc accgtcgaga ccggcaaggg
     6781 tcgagatctg tgtgcccagc cgcgcgaggt cgacgagcgc cgaggcgtgc tcggcggtca
     6841 ggtcaaccga cgggccggcg gccgtggcgc cgctgccggc cgaggccgcc gcgccagccg
     6901 cacctgcccc gcccgatcct tcgaggccag tcggggtgat caccggcgca tcgggccggt
     6961 aggccgggaa cagctcgtcc gtcttcgccg ggtcgccaag gcgcgcgtcg gcgaggagcc
     7021 ggaagatctc ggtgttcgcg ttgccgccga gcgaccacgc ctggaccttc tgccatgtcg
     7081 cgacgtcgag cggcgtccac ggctcgagct tgatgccgcc gatgccgccg gtgccggaga
     7141 ggagcccggc cacgacaaag gatgtcgaga gcttgccgtc gtgctcgctg atccaggcgt
     7201 tcacgccatc ggaatacgcc tgcaggacgg ccttcgcctc gggcgacatc gcggcgaggt
     7261 cgcgttccgc ggcgacacgc cagccgagtg ttcggatgta tttgtcgcga tcgatcgagg
     7321 tcttgccgaa cagctcggac agccggcccg cgccgatccg gcgcgagatc tccatctgcc
     7381 acatccgctc ctgggcatgg acgtagccct gggcgaggaa caggtcgtgg gggtcgccgg
     7441 cggtgatctg gatgagccct gatcgatctc gaacgacatt gacagtgtcg tgcaggcctg
     7501 cgatctcgat gctgccggtg gtctgcggcc agccgcgctg cgtgaggatc gcgccgaaga
     7561 ccatcacccc gatcagggcg agcgtgacga gcaggaaggt ccagcgaatc acagccgcga
     7621 tcagccgcac aacgaaccgt gcccctcgac ccatgtagcc ccctcagcgc ggcagtgtag
     7681 tgcccagacc cttggcgacc gcggctcgaa cgatggcgtc ggcgaagacg aggtccgtga
     7741 gcccgacgcc caggtggctg atcacgacac gtccggcggg gcggggcgtg ccggcgagga
     7801 gcgcctcgcc gatcgtcgtg ccggggtccg ggtagccatc gaagttgccg gcgtcgcggt
     7861 tggcgaggaa ctgctcgcgc tggtcgacga tgaacagcgc ggcatttctc gcgacctccg
     7921 cggcgcagta cgtcgcgtag tcgatgggca cgacggtggc atccgggcgg agccacgcat
     7981 tcgtcatcga ctggcgctcc tccttcggcc cgaaggacgc cgcggtcacg atcacgtccg
     8041 cgctctcgac ggccgctcgg gcggtctcgt gaacgatcgt ctcgccgatc cccgcggttg
     8101 cgtgcgcctc ggcggcgagc gcctcggctc gctcgggcga ccggtcgaac acgtggagcg
     8161 tggcgccggg caggacggcg ccgagcaggg gcaggtgcga acggccctgg acgccggcgc
     8221 cgatcagcgc gatgtaggtg cgtccgccgc cggccaaggc ggccgggccc caccgacgga
     8281 tggcgaggcc ggacacggcc gccgtccgtt ccgcggtgat cggtccgccg tcgagcatcg
     8341 cgaccggcac gcccgtgtcc gggtcgttca ggatgaccac ggcgctgatc gaggccatgc
     8401 cgagcgcgat gttcgacgcg tagccggcga cccacttcat gccgacaagg tcgttgctcg
     8461 cgtcggcgcc acgcaggtga gcgggcatgg cgtgcacgaa ggcatccgcc ggccgagggt
     8521 ggatcgcgat ctttggcggc agctcgctgg caccgggcgt cgcgagcgcg accatcgttc
     8581 gctcggcgag cgcgatgcgt tcctcgagcg gcggcatcgc cgcgaggacg tccgcagcgc
     8641 tgaaccatcg gagcgggggc agcgccatcg gggccgattg taggcagccg ccggtgcgct
     8701 tcggtcacgg atcggtacgc ttgccggtga tgccaccgat cgatgtcgcc cagagcctcg
     8761 agaacctcaa gctcgagcgc gacgcgatcg cgctgtacga cgcgctggcg aggatcgaga
     8821 aggacaggcg ccgggcggag gcgttccggt cgattgccgg caacgagcgc cggcacgcgg
     8881 aggtgtgggc gaaaaagctg cgcgcgctgg gggccacggt cccggagccg gacgggccgc
     8941 gccttcgcgt caggacgatc gtgctcctcg cgcgcctgtt cggcacccac gcagtccgcg
     9001 acctcgtcca ggccctcgag ggcgacgagg aggacgcgta caccgcgcag tcgacgcccg
     9061 aggtcgaggc gatcgccgcc gatgaacgcg agcacgccga gatctggaag cgcctcagcg
     9121 ggaccgaggg cgcgagcccg gcgttcgtcc ggcccgacgg attgggcccg ggcacgggcc
     9181 acaacttcac gggaccacgg gacatcgccg cccgcgaggg ctggcatcgg gccgggcggt
     9241 cggggacgct gcgggcggtc atcttcgggg tctcggacgg gctggtttcg aatctcgcgc
     9301 tcgtgatggg catcgccggc gcatcccagg gcgagggttc gttcgtgctc ctcgcgggca
     9361 tcgccggcct gctcgcgggc gcgttctcga tggccgccgg cgagtacgtc tcgatgcagt
     9421 cgcagcgcga gctgttcgag cgccagatcg cgctcgagcg ggccgagctc gaagcgatgc
     9481 ccgaggagga gcaggcggag ctcgcctcgg tctatcggtc gaagggcttc acccgcgaag
     9541 aggccgacac catcgcgaag cgcatcttcg ccgacccgaa gcacgccctc gatacgctgg
     9601 ttcgcgagga gctggggctc gaccccgacg agctcggctc ggcgtggggt gctgcgggcg
     9661 gctccttcgt cgcattcgcc attggggcac tgatcccggt ggtcccgtac ctgctcggcg
     9721 cgggcagcac cgcattcatc gcctcgatcg cgctatcgat cgccgccctg ttcgcggtcg
     9781 gcgccggcgt gagcctcctc accggccgcg ggctgctgtt ctccggttcg cgccaggtct
     9841 tcatcggcgc tgccgccgcg atcgtcacgt acctcgtcgg gcatgtgatc ggggtcgccg
     9901 tctgaggcgt cggcctcggc ctggatcggg acgccgtcga agtcgacatc gaccccatcg
     9961 acgatcaggc gatcgggtgg cggcagggtc aggtactcgc gatggatccg cagcacggcc
    10021 gcgacggtcc gggcatcgat atcgggctcg cgccgtccca gccgatcggc gatccatgtc
    10081 gcctcggcac gtcgctcgag ctcgccgagg tcctcctcgc gccggcgacc gtccatcgag
    10141 acgatcgcca cgaggcctcc gcccgcgacg aggccaacga gcaacagcca ccactggaat
    10201 tccgccatca ggccggaagg atagtgcccg gacgcccgca tacactcgcc agcgatgatc
    10261 gactggacgc cgaacccggt cctgttccag atcgggggct tccccctgaa ctggtacggc
    10321 attgcctatg ccgtcggcct cggggtcgcg tactgggtga tggttcgcca ggcgcggcgc
    10381 ctcggcgagg atccatcgat catcggcaac agcctcgtcg tgatcgccgt cgcggcgctc
    10441 atcggcgggc gcctgtacca cgtgatcgac cagtggcagc tctatgcgga ctgcccgatc
    10501 aagatcgtga tccccatcca gttcgaccag ggctgcagtg gctcgttcca cttcgccggc
    10561 ttcgccggtc tcggcgtgtt cggcggcttc atcaccggcg ccctggcgtt catcttcctc
    10621 gccacgcgct ggtacaaggt ctccgcgtgg cgctgggcgg acatcgtcgc gcccggcctg
    10681 ttcgcgatgc aggccatcgg gcgctggggc aacttcttca accaggagct gtacgggccg
    10741 ccgacgacgc tgccatgggg catcaggatc gactgcgccc accgcgtcgt cgatcccgcc
    10801 gccaacatcg actactcctg caccgcgttg ccgctggaaa ccacccactt ccacccgctg
    10861 ttcctgtacg aatccgtctc ggcgctgatc ggcctcgccg tgatcctttg gctcacgcgg
    10921 cgcccgccat cgtggctgcg gcgcggcgac ctcacgccga tcgcggtcat ctggatcggc
    10981 gccgcgcgct tcctgatcga gttcctgcgc atcggcaact ggcggctcgg cgagatcccg
    11041 acggcccaga tcatcggtgc ggggttcgtc gtgaccggcg tggccctgct gtggctgcgg
    11101 cgacgacagg acgcgccggc gtttctcgac ggtgtcgtgc cgatcgagga cgaggaccgc
    11161 gagggcgacg gcttcgacga cgatgacgat gaggatctcg aggacgacgg ctccgacgac
    11221 gatgacaccg acctcgacga cgacatcgac ggcgacatcg acggcgacga ggacgaacct
    11281 ccggccacgg ctgacgccga gtccccggcc ccgcccgcgg ctgacgccga gcccttgccg
    11341 ccgtcggcgg cccctggctg aatcccggcg tagtggcggc cgacccgcgc cggcgtcccc
    11401 gtgcccggct ccggccggag gccttggcgg cggcgcgcgg cggcgcacgt gagggcctcg
    11461 attggctggg ccgcgcgccc gaggcgaagg catccctgct gtaccgcgcc gtccgcctcg
    11521 ttacccgatt cgtgctgttc gggctcctcc gattccggat tgagacatcg ggccaggagc
    11581 acctgccgcg cggcggctac ctgttgatcg ccggcgccca ccgcggctgg atcgacccgt
    11641 tcgtcgcgat gcacgccgtg cccgccgagc cgcgctgctg gttcctcggc agcggcccct
    11701 cgacgttcac gtcacggtgg cgagaatcgc tcatccaccg cctcggcggc ctgctgccgg
    11761 tgtggcgcgg cggcctcggg atcgatggcc acgtccggtc ggcacgggcc gtggtcgagg
    11821 ctggggcagt cttcgtccag atgcccgagg ggaccatcaa cgggcctccg ggaacgctcg
    11881 ggccgttccg cccgggcgcc gcgctgattg cactccgaac cggcgcaacc atcgtgccgt
    11941 tggcgatggc cggcaccgag gagctgtacc tcggccgccg gatggcatct cgcatcctcc
    12001 cgtcgacgag cgtcgccgac ctgctcgggt cgagctggga cgggcagcga cctgaggagg
    12061 gctcgcgcgc ggagatggac ctggggcgcc gcgccagcga cgcgctggcc gcgcggatca
    12121 agcccgtcgt cgaggcgctc cagccgggca ccgtagaccc tcccgatcac ccacgtcggc
    12181 tgcggcgccg gctaacgtgg ctcctgctcg gccccgggcc ggtcgagcat tgaacgtcac
    12241 gattcgcgcg gaagggccag gcgaggcgga cgcggttcgc gccatcaacg acgccgcctt
    12301 cgggaagccg agtgatggag cgatcgtcga cgccatccgt gggaccgacc ggtggatcgt
    12361 gggtggctcg ctggtggccg aggatccgga cggagccctc gttggtcacc tgctcgcaag
    12421 tgaaggtgat ctggacatgc cggacgggaa gattcgtcgg atctggatgg tcggtcccgt
    12481 ggccgtcatc cccgcacggc aacgaaccgg tgtcggcagc gccctggtcc aagccgtgat
    12541 cgagctcgca acggagcgcg gccaaccgct gctgtgcctg ctggggcacc cgtcgtacta
    12601 cccccgattc ggcttcgagc cggcgcgtgc cctcgggatc gacccggccc ggccctggcc
    12661 cgacgaagcc tggatggtgc ggcggctacc cgcctggtcg ccgtcgctgc gcggggttgc
    12721 ccgtttcccg gacgcctttc cggactcaca gtaaccggct gcggctagac cggtcggagg
    12781 acgagcccga ccacgatggc gatgagcgcg ggcaccacct ggatcacggc ggcgcgcagg
    12841 aggcttggct tgtggaggta cagcacgacc ccggcggcaa ccatcgagcc gcaggcaaag
    12901 aggatgatcg cccggccacc cgccacgtcg ccggtcgcca gtaacgcgat gccgatggca
    12961 acgcccgcgg cgaggaacag gttgtagaag ccctggttgt acgcaaatga gcggacgacc
    13021 ttcgcgtcct cctcggactc gatgccgaac cgggcgtaca cgttcgggcg catgaaccgc
    13081 acgctctcga ggacgaagaa ccagacgtgg atcagcgcgg cgagcgccgc ggcttcgacg
    13141 gcgatgaggg acatggccgc attgtgcgcc ccgcgcggac gccgccgtga tgcggccgga
    13201 tatcctcgcg gcagtgaagt acgccgaatc catcctcgat ctcgtcggcg gcacgccgct
    13261 ggtgcggctg acccacatcc cccgtgacct cgggccgctc gacagccagc ccctgattct
    13321 cgccaagctc gagacgatga acccaggcgg ctcggtcaag gaccggatcg gcttgccgat
    13381 gatcgaagcc gccgaacggg ccggcttgct caagcccggc ggcacgatcg tcgagccgac
    13441 gtcgggcaat acgggccatg gcctcgcgat tgccgcggcg ctcaagggct accgctgcat
    13501 cttcgtgatg gccgacaagc agtcgcccga gaagcaggcc ctgctgcgcg cgtacggcgc
    13561 gcaggtcgtc ctctgcccga cgaacgtcga gcccgagtca ccggagagct actactccgt
    13621 cgccgctcgc ctggcgcgcg acatccccgg cgcgttcaag ccggaccagt actggaacca
    13681 ggagaacccg accgcccacg aagccacgac cggtcccgag ctctgggagc agaccgaggg
    13741 ccggatcacg catttcgtgg ccagcgtcgg caccggcggc acgatcacgg gcgccgcgcg
    13801 ctacctgcgc acgcagaagg
//
LOCUS       DATBSW010000113        11890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_185714, whole genome shotgun sequence.
ACCESSION   DATBSW010000113 DATBSW010000000
VERSION     DATBSW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185714"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1306)
                     /locus_tag="VIF63_03190"
     CDS             complement(71..1306)
                     /locus_tag="VIF63_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8238417.1"
                     /translation="MIDLRPTEENRLVQQSVREFTEAEILPHIREWDEKGEVHREVFG
                     RMADLGLLGAPIHAQWGGAGMDYISFAMICEELERADTAFRVVQSVHVGLNSLTLMQW
                     GTEEQKQRWLVPQAKGEKLATFGLTEPGVGTDAGNLASTARRDGDSYVLNGQKIWISL
                     ADLADHFLVFASVDRSKGHKGVTAFMLERGMQGLSTGTLHGKLGIRAGNTGLINMDDV
                     RVPADQRIGEDGEGFLIAMSAIDQGRYTVAAGNVGLAQACLDASLKYAHERRTFGDEI
                     GKHQLVKQMLAKMVAGIEAGRLLTWRAGFLKNHGVRNTRETSLAKWHATDHAVASALD
                     AIQIHGANGYSNEFPVERYLRNSKAAVIYEGTSQLHTLIQADFALGYREDRPLRCEPL
                     PAEGFERLPASVAAAAGRA"
     gene            complement(1303..2775)
                     /locus_tag="VIF63_03195"
     CDS             complement(1303..2775)
                     /locus_tag="VIF63_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8238418.1"
                     /translation="MLDRSSNEALHRQLVAGLRQAIASGRLRPSANLPGSRTLAHDLG
                     VARITVTTAYDQLVAEGYLDTDLRRGTRVSRDLPDRAFDVGGPGPTTPRGAFPTRNSW
                     APLAPVTMEDPLPDDEIDLGPESFSLGAVDRRAWERRLVAAWRELADEPAGAAVSYFG
                     GLGDPVLRHALADHLSVNRAVRARPDNVVITAGATAALAAVALVWLGPGRRCVVEEPG
                     GAQLRRSLASAGAEIVPVPVDTEGIDPAGLPEHADVVFVTPSWQYPSGGRLSVARRLG
                     LIEWARRAGALIVEDDCEGELRYAGDPLPTLAGLAEDGRVLYVNTFSKVLFPGLRTGY
                     VVVPDMVRGPLLAAIEAGARPPGALEQRALGRFVESGSYMRHLRRVRVRYGALRTTFE
                     RELRRHAGARLRVRSGEAGGHLLVEIGPGGPSATDLVGAARSVGVRFETLRANRLDRS
                     RGADDALVVYLTRASAAELATAAARLASCLDKLAPPMIRP"
     gene            3162..3968
                     /locus_tag="VIF63_03200"
     CDS             3162..3968
                     /locus_tag="VIF63_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238419.1"
                     /translation="MAADGTSVWVTSNATSELDRIDPATNTVVERITIDRGACGIAAG
                     PDGRIWVAVLGVGSVVAVDPTTSKVTARLDDVGPQLWDLKAGFDSIWVVDRSAKVLLR
                     IDPVTEKIRATIPVGPQPSGLAVMPDGIWVSDDTDGMVRRIDPATDKVVATADIDGTP
                     SWFADDGASNLVIAERGAGKVVALDPTTGTQGSPVAGWNEPLDGTVLGGAAWIPEGSG
                     RRLGVLDLAAEGDVIRYTLPGAINPFVAEPGFGDVWVLDFGGTTIWRIRP"
     gene            complement(4054..4239)
                     /locus_tag="VIF63_03205"
     CDS             complement(4054..4239)
                     /locus_tag="VIF63_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8238420.1"
                     /translation="MALNELGKMLTAAEVAEMLHLHVNTVKRLGDRGELKYYRVCKRG
                     DRRFLLEDVRRFLDNNR"
     gene            4444..5697
                     /locus_tag="VIF63_03210"
     CDS             4444..5697
                     /locus_tag="VIF63_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY8238421.1"
                     /translation="MLTETPGATLEAIRIVVALVAVAAVTALVVRRLRIPYSVSLVVL
                     GLVAGALLPAGSIRVTPELVLLVLVPGLVFEAALRIELAELRPTLGWMVLLAAPGVLI
                     SAAIVALVLNLATGLPFELGMVVGAMVAATDPVAVIATFRNLGTPRRLATLVEGESLF
                     NDGTALVLFIVTVGSITGSVSIGETAGAIVVAVASSILIGLAAGWLASRLIKLVDDRA
                     IELTISLAAAYGTYLLTDGLGQSGIIATVVAGVVIGTYGRAVGLSPRALEALDIVWDF
                     IAFLLTAFVFLLVGLAISFNDLLAAAPSIVAGLIAILVGRAVVVYGLLGGAARLVRGR
                     GRFSAVPIGWLHVLFWAGLRGAVAVAMALSLPEDFPQRSLLQEITFGIVLFTLFVQGT
                     TAERVVARVGAVELRERGPEGGAAS"
     gene            complement(5725..6372)
                     /locus_tag="VIF63_03215"
     CDS             complement(5725..6372)
                     /locus_tag="VIF63_03215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8238422.1"
                     /translation="MTTAALGRRTTPTALAVLAGGLPAIGLLGGLVVIVGGRWWALMN
                     GLDPLAVGAIFGLALAGLAVIGFRRIATQRAVGLGRRTWIALAIGVGAGLVLVGVTLA
                     GATIAGATLVPGLGRPAAPFVPWAAITIIVATGEEALLRGRLFNAVRHAGGLPAAVLL
                     TTFAFALMHVPLYGWHVVPLDFAVGLSFAGLRLATRGIVAPAAAHAVADLVTWWL"
     gene            complement(6369..7757)
                     /locus_tag="VIF63_03220"
     CDS             complement(6369..7757)
                     /locus_tag="VIF63_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8238423.1"
                     /translation="MTRTPRRPRSIVAIVALILALSALGSCTPAEYSVGAIRIGAVFP
                     ISSNAADLAGPELAGVQIAADLVNADGGIQGRRIELEVRDLRSRAEADGVMEDLRDQG
                     IEIVIGSYSSELSIAASEAADRAGLVYWEAGAVADRLTGRGLPRVFRVGASGANLGAN
                     SSSFAASELAARLGKAPSELRIALAVADDDYANSVADAAESTAVARGLPVVLRRGYNL
                     AVPDWPRVMDDLAAAKPDVIILASHIPDGIAFRQAMVASGLRVGALIGSTMAECDPDF
                     AGELGADAVGIFASDRPTAGFQPSALGPEAAAAYARFAPAWAKVASEAGGSGDSTGGR
                     GEYGDYGESEYTIHGPDEDATAEESEEALAGFSAAWALFQHVLPAAAGSDGTASLTAE
                     TVAATARSLDLPDGSLPNGAGVRFSSDPARLGQNERAAAVIWQWQAVRTYEFVWPPTY
                     ATGTIDFVPLDR"
     gene            complement(7754..8569)
                     /locus_tag="VIF63_03225"
     CDS             complement(7754..8569)
                     /locus_tag="VIF63_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238424.1"
                     /translation="MTDVASVRERRLGWLVVVAGGLLALAVQAAAPVGVPLFDGAPVV
                     EPYRFLHPVGTQAGEPTSFAAMPAVTGSKSPLLVAATTESPPQAQLIANEGAFELTPG
                     VTALKVEITPIEPPPAPVGAAIAGNAYRFSVTDQSGNPLKIASCDGCISLVLRTPDGI
                     VGEASIKRFANGGWQDVETLHAGVVAMYQTNPTVLGDMAVITGPTVPSGLDPLLLGGG
                     AVLLVVVGIIAFVLFRIQPASRPPTRGASGQIPSRVPSKRRSPKRPPPGRPKP"
     gene            complement(8566..8991)
                     /locus_tag="VIF63_03230"
     CDS             complement(8566..8991)
                     /locus_tag="VIF63_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238425.1"
                     /translation="MTPIRRSLVLSVIVAVALAACGEGVPTASPAPSLAPRPSSPAAV
                     AFVQPAQGSTVSGATIHVVLELTGATIVPETTTTLRPDEGHVHLYVDNQLVSMNYGLE
                     QDVPVTVGSHALKAEFVAADHAPFNPRVWSDEIVFTVTE"
     gene            complement(8988..10994)
                     /locus_tag="VIF63_03235"
     CDS             complement(8988..10994)
                     /locus_tag="VIF63_03235"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY8238426.1"
                     /translation="MRIARWRASAARPAALLSLLATVALLAAAVSPAAVSAHARLRSA
                     TPASGATLGSPPETVTLTFSETPDIRLTSIKVLDKGGVDRVSGAVEAVTDPPSSARVP
                     LQDLPDGVYTVSWRTVSAVDAHISAGTYVFGVGQAPPSTPPDTANAGTSQSGSPPAIV
                     ARWILYLGLMALMGAAWVAVAVARRPAPDLLAMAAAGWLLTAVGTVGVVAVQWAETGA
                     PIETLPSTSIGLSALVRVVSLGLIALALALLAVVSRFVGTRGWIVVGLTSAAALVVDV
                     GTGHAAAGDGWIPQVAAQALHGLSAGSWVGGLAALLVVLRTTPADERLATARRYSTWT
                     GISLAVLVVSGTVRAIAEIGTLDALFGTDFGRVVVAKSILLIGLAALGAFNRFFALRT
                     AARVATLLRRIGTAELTLAVVILGCSALLVNLSPPASAAGPIAPVAQPIVATGHDFGT
                     SMRARLVATPGAAGANDFDLAVSDYDSGEPVDASAVELRFELISTAGVAPSTLDLERT
                     AAGRFRASGSNLSIDGIWRVTATVTVRGSAADIPLVVATRIPPQDDAQLVTQGLPTLH
                     TIQLGAAGSVQVYIDPGTPGPNELHVTFFDPTGQEPLVVESVWIAAFPASGESSLPTP
                     RLLEPGHYVTSLNAVTGTLDVDALAPVPDGSGNVHLHVTLEVQP"
     gene            complement(11041..11778)
                     /locus_tag="VIF63_03240"
     CDS             complement(11041..11778)
                     /locus_tag="VIF63_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238427.1"
                     /translation="MHLRLRTRLFAGLSTTAFLLSLALAPSAAAHEGIQVGEYLLEIG
                     WRVEPAFVGQPNSVQVTIVDHHDESPITDLAVDALTVVVSTAGVDSPAIPLTPAFDAA
                     DGDGPLGEYGAALVPTSPGDYTFHITGAIHDTAVDLTLTSGEETFDPVEAPGELEFPV
                     KQPTMLEVATRLDRIDGRIEELQGSVPNGQLIGDLRTAASDARAAAESAMGLATIGIA
                     VGVMGLAFGAVGVWLALRAGRKGTASA"
BASE COUNT         1744 a         4204 c         4255 g         1687 t
ORIGIN      
        1 gagtccgcag ccggaacgaa ggtcgaggcg agaccgcgga gcgtcgccag ctcggcgggg
       61 ctgaacgtgc tcaggcgcgc ccggcggcag ccgcgaccga ggccggcagg cgctcgaagc
      121 cctcggccgg cagcggctcg cagcgcagcg gccggtcctc gcggtatccg agcgcgaagt
      181 cggcctggat cagggtgtgg agctggctgg tgccttcgta gatcacggcg gccttcgagt
      241 tgcgcaggta gcgctcgacc ggaaactcgt tcgaatagcc attggcgccg tggatctgga
      301 tcgcgtcgag cgcgctcgcc accgcgtggt ccgttgcgtg ccacttggcc agcgacgtct
      361 cgcgcgtgtt ccgcacgccg tggttcttga ggaagcccgc gcgccaggtc aggagccgcc
      421 cggcctcgat gcccgcgacc atcttcgcga gcatctgctt caccagctgg tgcttgccga
      481 tctcgtcgcc gaacgtgcgg cgctcgtggg cgtacttgag cgaggcatcg aggcacgcct
      541 gggcgagccc gacgttgccc gctgcgaccg tgtaccggcc ctggtcgatg gcgctcatcg
      601 cgatcaggaa gccctcgcca tcctcaccga tccgctggtc ggccggcacc cggacgtcgt
      661 ccatgttgat caggccggtg ttgccggcac gaataccgag cttgccgtgg agcgtgccgg
      721 tcgacagccc ctgcatgccc cgctcgagca tgaacgcggt cacgcccttg tgacccttcg
      781 accgatcgac cgacgcgaac acgaggaagt ggtccgcgag atcggcgagc gagatccaga
      841 tcttctggcc gttgaggacg taggaatcgc cgtcgcgccg cgccgtggac gccagattcc
      901 cggcatccgt gcccacgccc ggctcggtca gcccgaacgt tgcgagcttc tcacccttcg
      961 cctggggcac cagccagcgc tgcttctgct cctcggtccc ccactgcatg agcgtgagcg
     1021 agttgagccc gacgtggacg ctctgcacga cccgaaacgc cgtatcggcg cgctcgagct
     1081 cctcgcagat catggcgaag ctgatgtagt ccatcccggc gccgccccac tgggcgtgga
     1141 tcggggcgcc gagcaggccc aggtcggcca tgcgcccgaa gacttcacga tggacctcgc
     1201 ccttctcgtc ccactcgcgg atgtggggca ggatctcggc ctcggtgaac tcgcgcaccg
     1261 actgctggac gagccggttc tcctcggtcg ggcgaaggtc gatcacggcc ggatcatagg
     1321 cggcgcaagt ttgtcgaggc agctcgcgag gcgcgctgcg gcggtggcga gctcggctgc
     1381 cgaggcgcgc gtcaggtaca cgaccagcgc gtcgtcggcg ccacgcgacc gatcgaggcg
     1441 gttcgcccgc aaggtctcga agcgcacgcc aaccgacctc gcagcaccga cgagatcggt
     1501 cgccgacggc ccaccggggc cgatctcgac gagcaggtga ccaccggcct cgccggatcg
     1561 gaccctgagc ctggcgcccg cgtgccgccg cagctctcgc tcgaacgttg tgcgcagggc
     1621 gccataccga acgcggaccc gccgcaggtg acgcatgtac gaacctgatt cgacgaaacg
     1681 gccgagcgct cgctgctcga gggcgccggg tggccgcgca ccggcctcga tcgccgccag
     1741 gaggggcccg cgaaccatgt ccggcaccac gacatagccg gttcggaggc cgggaaacag
     1801 caccttcgag aacgtgttga cgtacagcac gcggccgtct tcggcgaggc cggcgagggt
     1861 cggcagcgga tctccggcgt agcgcagctc gccctcgcag tcatcctcga cgatgagcgc
     1921 gccggcgcgc cgagcccatt cgatcagccc aagccggcgc gcgaccgaga gtcgcccgcc
     1981 gctcgggtat tgccacgagg gcgtcacgaa cacgacatcg gcgtgctccg gaagcccggc
     2041 cggatcgatg ccctcggtgt cgacgggcac gggcacgatc tccgcccccg cactcgcgag
     2101 actccggcgg agctgcgcgc cgcccggctc ctcgacgacg caccggcggc ccggtccgag
     2161 ccagacgagc gccacggcgg cgagcgcggc cgtcgcgccg gcggtgatga ccacgttgtc
     2221 cggacgcgcg cgcaccgccc gattcacgga caggtggtcg gccagggcgt gtcgcagcac
     2281 gggatcgccg aggccaccga agtaggaaac ggcagcgccg gccggctcgt ccgcgagctc
     2341 tcgccaggct gccacgaggc gccgctccca cgcccggcgg tcgacggccc cgagcgagaa
     2401 ggactcaggc cccaggtcga tctcgtcgtc gggcagcggg tcttccatcg tcaccggcgc
     2461 gagcggcgcc cacgaattgc gcgtcgggaa ggcgcctctc ggcgtggttg gcccgggccc
     2521 tccgacgtcg aacgcccgat ccggcaggtc gcggctcacc cgcgtcccgc gtcgcaggtc
     2581 ggtgtcgagg tacccctcgg ccacgagctg gtcgtatgcc gtcgtcaccg tgatccgggc
     2641 gacaccgaga tcgtgcgcca gggtccggct gccgggcaaa ttggcgctcg ggcgcaggcg
     2701 gccgctggcg atggcctggc gcagccctgc gacgagctgg cggtggagcg cctcgtttga
     2761 cgaccgatca agcaccagcg gcaggcctgc atggctcgac tggccggtac gacgccgcct
     2821 ggccctatcg atctcgcgtg aactggcact tgcgatcggt ccagccatgg cgtggagtat
     2881 ccaccatgtc cagcgccttg tgcgtccggc gtggccggcg tcccgcgccc gcccggcagg
     2941 cattgtcagc cctcgtcctt gccgctctac tcggcgcatg cggcggtgcg cagccgacgc
     3001 ttgcgccatc ggcgcctcca accgcaagct cgacgcccgc tcctgtcgtc acaacgtcac
     3061 caacgggctc gcgggcacca acccccatcc cggtcccgac gggcacgccc ctcgacgacc
     3121 ggctcgtcgc aacgatcacc gtcgcctccg cgccatgcgc gatggccgcc gacggcacct
     3181 cggtctgggt gacgagcaat gccacgagcg agctggaccg catcgatccg gcgacgaaca
     3241 cggtcgtcga gcgaatcacg atcgatcgcg gcgcctgcgg catcgccgcc ggcccggatg
     3301 gccgaatctg ggtcgccgtg ctgggcgttg ggtccgtggt cgccgtcgat ccgacgacgt
     3361 ccaaggtcac cgcgcggctc gacgacgtcg gcccgcagct gtgggatctg aaggccggct
     3421 tcgactcgat ctgggtcgtg gacaggagcg cgaaggtgct gctccggatc gacccggtga
     3481 ctgagaagat ccgcgccacg atccccgtcg ggccgcagcc gtccgggctc gcggtcatgc
     3541 ccgatggaat ctgggtcagc gacgacaccg acggcatggt tcgccggatc gacccggcga
     3601 ccgacaaggt cgtcgcaacc gccgatatcg atggcacgcc gtcctggttc gcggacgatg
     3661 gcgcatcgaa cctggtcatc gcggaacgcg gcgcgggtaa ggtggtcgcg ctggatccga
     3721 caacgggaac ccagggcagt ccggtagctg gttggaacga gccgcttgat ggaaccgtgc
     3781 tcggcggtgc cgcctggatc ccggagggca gcgggcgacg gctgggcgtg cttgacctcg
     3841 ccgccgaggg cgatgtgatc cgctacaccc tgcctggagc gatcaatccg ttcgtggccg
     3901 agccgggctt cggcgatgtc tgggtgctcg atttcggcgg gacgacgatc tggcgcattc
     3961 gcccctgagg acgccagaaa gcgccggtgg tgtcaccagc gctttcgatt tcgcagggcg
     4021 gcgtgcagcg ccgccgaact atcggttgtc gagctagcgg ttgttgtcga ggaagcgccg
     4081 gacgtcctcg aggaggaacc ggcggtcgcc gcgcttgcac acgcggtagt acttcagctc
     4141 gccgcgatca cccagccgct tcaccgtatt gacgtggaga tgaagcatct ccgcgacttc
     4201 ggcggcggtg agcatcttgc ccagctcgtt cagtgccatc gagcccatcc gtgcattgcc
     4261 tttcgagacg acgatcgaac attcctgcgg cgcagtagct accccgcgac gaatggctgc
     4321 gaccatgact cgtcgtcgat ctggacctta cgaacgggcg ggtgtgcttc caagccgtac
     4381 ttcggtccgg tgcggggcag taccctccgg cgagcaccag tctggtgcag tccgcaggag
     4441 gcgatgctga cagagacgcc aggcgccacc ctcgaggcga tccggatcgt cgtcgcgctc
     4501 gtggcggtcg ccgcagtgac cgcactggtt gtccgacgcc tccggatccc ctacagcgtt
     4561 tcgctggtgg tgctgggcct ggtcgcaggc gccctcttgc cggcgggttc gatccgcgtc
     4621 acgccggagc tcgtgctcct cgtcctcgtg cccgggctgg tgttcgaagc tgccctccgc
     4681 atcgagctcg ccgagctgcg cccgaccctc ggctggatgg tgctgctcgc cgcgcccggc
     4741 gtcctcatct cggccgcgat cgtggcgctc gtgctgaatc tcgccacagg gctgccgttc
     4801 gagctgggca tggtggtcgg cgcgatggtg gccgcgacgg acccggtcgc ggtcatcgcg
     4861 accttccgca acctcggcac gccacgccgg ctggcgacgc tggtcgaagg cgagagcctg
     4921 ttcaacgatg gcacggcgct cgtgctgttc atcgtcacgg tcgggtcgat cacgggcagc
     4981 gtctcgatcg gtgagacggc gggagcgatt gtcgtcgcgg ttgcgagcag cattctcatc
     5041 gggctcgcgg cgggctggct cgcgtcgcga ctgatcaagc tcgtcgacga ccgcgcgatc
     5101 gagctgacga tctcgctggc agcggcatac ggcacctacc tgctcaccga cggcctcggg
     5161 cagtcgggga tcatcgcgac cgtcgtcgcc ggcgtggtga tcgggacgta cgggcgcgcg
     5221 gtcggcctca gtccccgtgc gctcgaagcg ctcgacatcg tctgggactt catcgcgttc
     5281 ctcttgaccg cattcgtgtt cctcctcgtc ggcctggcga tctcgttcaa cgacctgctc
     5341 gcggctgcac cctcgatcgt ggcggggctc atcgcgatcc tcgtcggccg tgcggtcgtg
     5401 gtctacgggc tgctcggcgg tgcggcgcga ctcgtccgcg ggcgcggccg gttcagcgcc
     5461 gtgccgatcg gctggctcca cgtgctgttc tgggccggcc tccgcggcgc cgtggcggtg
     5521 gcgatggcgc tgtccctgcc cgaggacttc ccgcagcggt cgctgctcca ggagatcacc
     5581 ttcggtatcg tgctcttcac gctgttcgtg cagggcacga cggccgagcg cgtggtcgcc
     5641 cgggttggcg cggtcgagct gcgggagcgt ggcccggagg gcggcgcggc gagctgaccc
     5701 gccgcccttc tgcggctcag gccgctacag ccaccacgtc acgaggtcgg cgacggcgtg
     5761 cgccgcggcc ggcgcgacga tgccacgcgt cgcgagccgc aggccggcga agctcagccc
     5821 gaccgcgaag tcgagcggaa cgacgtgcca gccgtacagc gggacgtgca tcaacgcgaa
     5881 ggcgaacgtg gtgaggagca cggcagccgg caggccgccg gcgtggcgaa ccgcattgaa
     5941 cagccggccg cggagcagcg cctcctcgcc cgttgcgacg atgatggtga tcgccgccca
     6001 cggaacgaac ggcgctgcag gccgcccgag gcccggcacc agggtggcgc ccgcgatcgt
     6061 ggcgcccgcg agggtcacgc ccaccagcac caggccggcg cccacgccga ttgcgagcgc
     6121 gatccacgtc cgtcgaccga gtcccactgc gcgctgcgtg gcgattcgcc tgaaaccgat
     6181 gacggccagc cccgccagcg ccagcccgaa tatcgcgccg acagcgagtg gatcgaggcc
     6241 gttcatcaga gcccaccagc ggccgccgac gatcacgacc aagccgccga gcaggccgat
     6301 cgccggcagc ccgccggcga ggaccgccag cgcggtgggc gtggtgcggc gacccagggc
     6361 ggcggtcgtc atcggtcgag gggcacgaag tcgatcgtgc ccgtggcata cgtcggcggc
     6421 cagacgaact cgtaggtccg cacggcctgc cactgccaga tgactgcagc ggcccgctcg
     6481 ttctggccga ggcgcgccgg atcggaggag aatcggacac ccgcgccgtt cggcagcgag
     6541 ccgtccggca ggtccagcga gcgagccgtg gcagcgaccg tctccgcggt gaggctggcc
     6601 gtcccgtccg aacctgcggc cgcgggcagg acgtgctgga acagcgccca cgcggccgag
     6661 aagccggcga gcgcttcctc ggactcctcc gccgtcgcgt cctcgtccgg cccgtggatc
     6721 gtgtactcgg actcgccgta gtcgccgtac tcgccccgac caccggtcga gtccccggag
     6781 ccacccgcct cgcttgcgac cttggcccac gccggggcga atcgcgcata cgcggcggcc
     6841 gcctccggac cgagcgcaga cggctggaat cccgccgtcg gccgatccga ggcgaagatc
     6901 ccgaccgcgt ccgccccgag ctcgccggcg aagtcgggat cgcactcggc catcgtcgag
     6961 ccgatgagcg ccccgacccg aagcccgctc gcgaccatcg cctgccggaa tgcgatgccg
     7021 tcggggatgt gcgaggcgag gatgatcacg tcgggcttcg cggcggcgag gtcatccatg
     7081 acccgcggcc agtccgggac ggcgaggttg tagccccggc ggagcaccac gggcagcccg
     7141 cgagcaaccg ctgtcgattc cgcggcatcg gcgacgctgt tcgcgtagtc gtcgtcggcc
     7201 acggcaagcg cgattcgcag ctcggacggc gccttgccga gccgcgcggc cagctcggag
     7261 gccgcgaacg aggacgagtt cgcgccgagg ttggccccgc tcgcacccac ccggaaaaca
     7321 cgcggcagcc cgcggcccgt cagccggtcg gcgaccgcgc cggcctccca atagacgagc
     7381 cctgcgcgat cggccgcctc gctggcggca atcgagagct cggacgaata cgacccgatc
     7441 acgatctcga tcccctggtc gcgcaggtcc tccatgacgc cgtctgcctc ggcgcgcgag
     7501 cgcaggtcgc ggacctccag ctcgatgcgc cggccttgga ttccgccgtc ggcattcacg
     7561 aggtcggcgg cgatctggac gccggcgagc tccggcccgg ccaggtcggc ggcgttgctg
     7621 gagatcggga acacggcgcc gatgcggatg gccccgacgc tgtattcggc gggcgtgcag
     7681 ctccccagcg ctgacagcgc aaggatgagc gcaacgatgg cgacgatcga acgggggcgc
     7741 ctcggcgtcc tggtcacggc ttcggcctcc cgggtggcgg ccgcttcggc gaccggcgtt
     7801 tcgatggcac acggcttggg atctgccccg acgccccgcg cgtcggcggg cgcgaagccg
     7861 gttggattcg gaacaggacg aaggcaatga tcccgacgac aacgagcagc acggcgccac
     7921 cgccgagcag cagcggatcc agcccggatg gaaccgtggg cccggtaatc actgccatgt
     7981 cgcccagcac cgtcgggttc gtctggtaca tcgcgacaac cccggcatgg agcgtctcga
     8041 cgtcctgcca ccccccgttg gcgaagcgtt tgatcgatgc ctcgcccacg atcccgtcgg
     8101 gcgtccggag cacgagcgag atgcagccgt cgcaggacgc gatcttcagc gggttcccgg
     8161 actggtcggt gacgctgaac cggtaagcat tgcccgcaat cgccgcgccg acgggcgcgg
     8221 gcggcggctc gatcggcgtg atctcgacct tcagcgccgt cacgcccggt gtcagctcga
     8281 acgcgccttc gttggcgatc agctgcgcct gcggcggact ctccgtcgtg gcggcgacga
     8341 gcaggggcga cttgctgccc gtgaccgcgg gcatcgcggc aaagctcgtg ggctcgcccg
     8401 cctgcgtgcc gaccgggtgg aggaaccgat acggctccac gaccggcgcg ccgtcgaaca
     8461 gcggcacgcc gacgggcgcc gcagcctgga cggcgagcgc gagcagtccg ccggcaacga
     8521 cgaccagcca gccgaggcga cgctcgcgga cggacgcgac gtcagtcatt cggtcaccgt
     8581 gaacacgatc tcgtccgacc atacccgcgg attgaacggc gcgtggtcgg cggcgacgaa
     8641 ctccgccttc agggcgtgcg agccgaccgt gaccgggacg tcctgctcga gcccgtagtt
     8701 catcgacacc agctgattgt ccacgtacag gtggacgtgt ccctcgtccg ggcggagcgt
     8761 ggtcgtcgtc tccggcacga tcgtcgcgcc ggtcagctcg aggacgacgt ggatggttgc
     8821 gccgctgacg gtcgagccct gcgcaggctg gacgaaggcg acggctgccg gcgacgacgg
     8881 ccgcggggcc agcgacggcg ccggcgacgc ggtgggtacg ccttcaccgc atgcggcgag
     8941 ggcgactgcg acgatcaccg agaggacgag cgagcggcgg atcggtgtca tggctggacc
     9001 tcgagggtga cgtggaggtg gacgttgccc gagccatccg gcacgggcgc aagggcgtcg
     9061 acatcgagag tcccggtgac ggcgttgagg gacgtgacgt agtgcccggg ctcgagcagg
     9121 cgcggcgttg gcaggctgct ctcgccgctc gccgggaaag cggcgatcca gaccgactcg
     9181 accaccagcg gctcctggcc tgtcggatcg aagaaggtga cgtggagctc gttcgggccg
     9241 ggcgttccgg ggtcgatgta gacctggacc gagccggcgg cgccgagctg gatcgtgtgg
     9301 agcgtgggca gcccctgcgt gaccagctgc gcatcgtcct gtggcgggat ccgcgtcgcg
     9361 accacgagtg ggatatcggc cgcgctgccg cgcaccgtga cggtcgcggt cacccgccag
     9421 atcccgtcga tggagaggtt cgagcccgac gcccggaatc gaccggcggc cgtgcgctcg
     9481 aggtcgagcg tcgacggcgc gacgccggcc gtcgagatga gctcgaaccg cagctcgacg
     9541 gccgatgcgt cgaccggctc gccgctgtcg tagtcgctca ccgcgaggtc gaagtcgttg
     9601 gcgcccgcgg cgcccggcgt cgcgacgagt cgcgcccgca tgctcgtgcc gaagtcgtgc
     9661 cccgtcgcga cgatcggttg cgcgactggc gcgatcggtc ccgcggcgga ggccggcggt
     9721 gagaggttca cgaggagcgc gctgcagccg aggatcacca cggcgagggt cagctcggcg
     9781 gtcccgatgc ggcgcaggag ggtcgcgacc cgcgcggcgg tccgcagcgc gaagaaccga
     9841 ttgaatgccc cgagcgcggc gaggccgatg agcaggatgc tcttcgcgac gacgacccgg
     9901 ccgaagtcgg tgccgaagag ggcgtcgagc gtgccgatct ccgcgatcgc gcgcacggtc
     9961 ccggagacga cgagcacggc cagggagatc ccggtccaag tcgaatagcg tcgcgcggtc
    10021 gccagccgct cgtcggctgg tgtcgtgcgc aggacgacga gcagggccgc gaggccaccg
    10081 acccacgacc cggcgctgag cccgtgcagc gcctgcgccg ccacctgcgg gatccagcca
    10141 tcacccgccg ccgcgtgtcc ggtgccgacg tcgacgacga gcgcggccgc tgatgtcagc
    10201 cccacgacga tccagccgcg ggtgcccacg aaacgcgaaa cgacggcaag caacgcaagg
    10261 gcgagcgcga tgagcccgag cgacacgacg cggacgagcg cggacaggcc gatcgatgtc
    10321 gacgggagcg tctcgatcgg ggcgcccgtc tcggcccact ggaccgcaac gacgccgacg
    10381 gtgccaacgg cagtcaggag ccagccggcc gccgccatcg cgagcaggtc cggcgccggg
    10441 cggcgggcga ccgcgacggc gacccacgcc gcccccatga gcgccatcag tcccaggtac
    10501 aggatccagc gtgcgacgat cgcgggcggc gatccgctct ggctcgtgcc ggcatttgcc
    10561 gtatccggcg gcgtcgatgg cggcgcctgg cccacgccga agacgtacgt cccggccgag
    10621 atgtgcgcat cgaccgccga gaccgtccgc cacgacaccg tatagacgcc gtcggggagg
    10681 tcttggagcg gcacccgagc ggacgacggt ggatcggtga ccgcctccac cgctccgctg
    10741 acgcggtcca cgccgccctt gtcgagcacc ttgatcgacg tcaggcggat gtccggcgtc
    10801 tcgctgaagg tcagcgtcac cgtctccggc gggctgccga gggtcgcgcc cgacgcgggc
    10861 gtcgcgcttc ggagccgggc atgcgccgac accgcagccg ggctcaccgc ggcggcaagg
    10921 agcgcgaccg tggccagcag cgacaggagt gcagccgggc gggccgcgct cgctcgccac
    10981 cgcgcgatcc tcatcgctgg ctcgatccca cgctgatcgg gagctcgttc gtctcgaccc
    11041 tcaggccgag gcggttccct tgcgaccggc gcgcagcgca agccacacgc cgaccgcgcc
    11101 gaacgcgagg cccatgaccc caaccgcgat cccgatcgtc gcgaggccca tcgccgattc
    11161 agcagccgct cgcgcgtcgg atgccgccgt ccgcaggtcg ccgatcagct ggccgttcgg
    11221 gaccgagccc tggagctcct cgatgcgccc gtcgatgcga tcgagacgcg ttgcgacctc
    11281 gagcatcgtc ggctgcttga ccgggaactc gagctcgccg ggcgcctcga cgggatcgaa
    11341 ggtctcctca ccggaggtca gcgtcaggtc gaccgccgtg tcgtggatcg cgcccgtgat
    11401 gtggaacgtg tagtcgccgg gcgatgtcgg gacgagcgcg gcgccgtact cgccgagcgg
    11461 accgtcccca tctgcggcat cgaacgccgg tgtcaggggg atggccgggc tgtcgacccc
    11521 cgccgtcgag acgaccacgg tgagggcatc gacggcgagg tcggtgatcg gtgactcgtc
    11581 gtgatggtcg acgatcgtca cctggacgct gttgggctgg ccgacgaagg cgggctcgac
    11641 ccgccagccg atctcgagga ggtactcgcc gacctggatg ccctcgtggg cagcagccga
    11701 tggcgcgagg gcgagcgaga gcaggaacgc ggtggtcgag aggccggcaa acaaccgggt
    11761 ccgaaggcga aggtgcattt catacctccc aggcgggtgg ttgagcacga ggtgcccgac
    11821 cggcgcgtgg gggttggtgc tgctccggtt cgggctagac ggcgaccacc gcgggtggtg
    11881 cgcgcagcgc
//
LOCUS       DATBSW010000114         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_890657, whole genome shotgun sequence.
ACCESSION   DATBSW010000114 DATBSW010000000
VERSION     DATBSW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890657"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VIF63_03245"
     CDS             complement(<1..715)
                     /locus_tag="VIF63_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238428.1"
                     /translation="MNIRSRTLAVALAAAFPIAAQATDGYFSHGYGMQAKGMGGASTA
                     VAESAFGAATNPATMAFAGNRWEIGLDMFSPWRKASRSGGAQYGLDGSADSDSNYFFI
                     PEFAYNKMISPSMSLGLTLYGNGGMNTDYPGGQLPSPGACGPATGPGTGFNPQPGPYN
                     LFCGNGSLGVDLSQLIVAPSAAFKLGADHSIGVAPLFAYQRFEMNGIQPFNGFSTSPG
                     NVSNNGYDSSTGWGVRVGYY"
     gene            complement(730..1353)
                     /locus_tag="VIF63_03250"
     CDS             complement(730..1353)
                     /locus_tag="VIF63_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY8238429.1"
                     /translation="MDDTVRPDPELIERMVRERVDAILDERLEAKLAALRESKPASMT
                     IIATKGTLDWAYPPFILASTAAALGWNVAIFFTFYGLELLKKDLSGLQVSPLGNPAMP
                     MKMPFGPDWFRSIRWNIPNAMQAIVPGFEAFATTMMKKTIGNKGVASISELREACIEA
                     DVQLIGCQMTVDLFGYRHEDFVPQVKEYVGAATFLPMAKDADVTLFI"
BASE COUNT          247 a          484 c          543 g          281 t
ORIGIN      
        1 cgtagtagcc cacgcgcacg ccccacccgg tcgagctgtc gtagccgttg ttgctcacgt
       61 tgccggggct ggtcgagaag ccgttgaaag gctggatgcc gttcatctcg aagcgctggt
      121 aggcgaagag gggcgccacg ccgatggagt ggtcggcccc gagcttgaag gccgcgctgg
      181 gcgccacgat gagctgcgag aggtccacgc cgaggctgcc gttgccgcag aacaggttgt
      241 acggcccggg ctgcgggttg aagcccgttc cggggccggt ggcggggccg catgcgccgg
      301 gcgacggaag ctgcccgccg gggtagtcgg tgttcatgcc gccgttgccg tagagcgtga
      361 gcccgagcga catcgatggc gagatcatct tgttgtaggc gaactcggga atgaagaagt
      421 agttgctgtc gctgtcggcc gagccgtcca ggccgtactg ggcgccgccg ctgcgcgagg
      481 ccttgcgcca cgggctgaac atgtcgaggc cgatctccca gcggttgccc gcgaaggcca
      541 tcgtggcggg gttggtcgcc gcgccgaacg cggactcggc gacggccgtg gacgcgccgc
      601 ccatgccctt ggcctgcatg ccgtagccgt gcgagaagta gccgtcggtc gcctgcgcgg
      661 caatcgggaa ggcggccgcg agcgcgaccg cgagtgtcct ggaccggatg ttcatcgtga
      721 ttgtcctgtc tagatgaaaa gggtgacgtc ggcgtccttc gccatcggca ggaaggtcgc
      781 ggcgcccacg tactccttga cttgcggcac gaagtcctcg tgccggtaac cgaagaggtc
      841 caccgtcatc tggcagccga tcagctgcac gtcggcctcg atgcacgctt cgcgcagctc
      901 cgagatggag gccacgccct tgttgccgat ggtcttcttc atcatcgtcg tggcgaaggc
      961 ctcgaagccc ggcacgatcg cctgcatcgc gttgggaatg ttccagcgga tcgacctgaa
     1021 ccagtcgggg ccgaagggca tcttcatcgg catggccggg ttgcccagcg ggctcacctg
     1081 aaggcccgag aggtccttct tgaggagctc gaggccgtag aacgtgaaga agatcgcgac
     1141 gttccagccc agcgccgccg cggtcgaggc gaggatgaac ggcggatacg cccagtcgag
     1201 ggtgcccttg gtggcgatga tggtcatcga ggcgggcttc gattcgcgca gcgccgcgag
     1261 cttcgcctcg agccgctcgt cgaggatggc gtcgacgcgc tcgcgaacca tgcgctcgat
     1321 caattccgga tcgggtctga cggtgtcgtc catggaaggc cctccctgtg cgctcaacga
     1381 tccatcgtgt accgcggcgg ccatgggcgg gttgatgccg ggcaagccct cccttcgcgg
     1441 atgcccgatc ctagcggggt cgcgccatat atgcaacttt cataatatta tcgacttcta
     1501 atatgacggc cggcctcgag gatccgcatg tcctcgatcc ctatttgctg gagcc
//
LOCUS       DATBSW010000115         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_820462, whole genome shotgun sequence.
ACCESSION   DATBSW010000115 DATBSW010000000
VERSION     DATBSW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820462"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VIF63_03255"
     CDS             <1..501
                     /locus_tag="VIF63_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8238430.1"
                     /translation="VLARTRADRLFAIAQRILRDVDRAEDAMQQALVIAWRELPRLRD
                     PDRFDAWLNRLLVNACYAEARQRRDWSANIRLLPVDGPQVEDGTRQVADRDELERGFR
                     RLPPEQRAILVLHHYLGLNPIEVAETLDIPTGTARSRLHYAHRAMRAALEAVERSSVI
                     AGKGIS"
     gene            498..1337
                     /locus_tag="VIF63_03260"
     CDS             498..1337
                     /locus_tag="VIF63_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238431.1"
                     /translation="MSGRTEIERALDGFLAEGPETVKDQALMRALDAIDRTNQRRGPF
                     ASWRFPHMNTYSRIAVATVVAVIAIGGALYLIGPRNGVGSPTAVPSPTQPAPAPTASV
                     AVAATNPPSAAKSTNLDTTTWTAFTSTRYGYSAAYPGPWTAAVATENWIFETRQQFWS
                     SNGDGADRFISSGFPQFHLTAFSSTMPAQTTPEGWIDSYINMGAACPSALNLPVVQID
                     GHQGRISANCAMAKADPQPSPVVEVFVAVGQTMFVFGIDDYDDVLLRAFLKTVTLPAP
                     AAS"
     gene            1445..1939
                     /locus_tag="VIF63_03265"
     CDS             1445..1939
                     /locus_tag="VIF63_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238432.1"
                     /translation="MSSTSPSRRMRVTRSASLAMLMLAVVVGTAFATGASGFRGTPLA
                     RGTTAGPMHFNVGDIKFQTKAPVDFAHATVTIDPLGSSGWHSHPGVVLVTVSAGTVTF
                     YDADCSFNTYPVGSSFVESNGTTGLARNESASVTAVVYVTYIVPAGAPALRIDQPNPG
                     CAQS"
BASE COUNT          359 a          780 c          638 g          332 t
ORIGIN      
        1 gtcctggctc gaacccgtgc cgaccggctc tttgcgatcg cacaacggat cctccgcgac
       61 gtcgatcggg ccgaggatgc gatgcagcag gcactggtga tcgcctggcg cgagctgccc
      121 cgcctccgcg atccggaccg attcgacgcg tggctcaacc gcctcctcgt caacgcctgc
      181 tacgccgagg cccgccagcg gcgggactgg agcgccaaca tccgcctcct gccggtcgac
      241 ggaccccagg tcgaggatgg gacgcgccag gtcgccgatc gcgacgagct cgaacgtggc
      301 ttccggcgac tgccgccgga acagcgggcc atcctcgtcc tgcaccacta cctcgggctc
      361 aatccaatcg aggttgccga gacgctcgac atcccgaccg gcacggcccg atctcgactc
      421 cattacgccc accgagcgat gcgtgccgcc ctcgaggcgg tcgaacgctc ctcggtcatc
      481 gcgggaaagg gcatctcatg agcggccgaa ccgagatcga acgcgccctc gacggcttcc
      541 tcgccgaagg cccggaaacg gtcaaagacc aggcactcat gcgtgcgctc gacgcgatcg
      601 accgaaccaa ccagcgacgc ggcccgttcg cgtcgtggag gttcccgcac atgaacacct
      661 attcccgcat cgcggtggcg accgtcgtgg ccgtcatcgc catcggcggc gcgctgtatc
      721 tcatcggtcc acggaacggc gttggcagcc cgaccgccgt gccgagccca acacagccag
      781 cgccggctcc aaccgcgtcc gtggccgtcg cagccacgaa tccgccgtcc gcggccaagt
      841 ccaccaacct ggataccacg acatggacgg cattcacgtc cacgagatac ggctattccg
      901 cggcctatcc ggggccatgg accgcggccg tggcgaccga aaactggatc ttcgagaccc
      961 ggcagcagtt ctggtcatcg aacggcgacg gagccgaccg gttcatcagc agcggctttc
     1021 ctcagtttca tctgacggcg ttttcgtcga ccatgccggc gcagacgacg ccggaaggct
     1081 ggatcgactc gtacatcaac atgggagcgg cgtgcccaag cgccctcaat ctgccggtcg
     1141 ttcagatcga tggccatcaa gggcggatct cggcgaactg cgccatggcg aaggccgatc
     1201 ctcagccttc gcctgtcgtc gaggtattcg tcgccgtcgg gcagacgatg ttcgtcttcg
     1261 gcatcgacga ctacgacgac gtcctgctcc gagccttcct gaagacggtc acgctgccgg
     1321 ctcccgccgc cagctgaccg ctcgccacga atagtgaatg ccggagcccg ctccggcccc
     1381 atagatgccg gatccaaggt gaatcgccgg catcgctgac caagcttcac gaaggagcca
     1441 ctccatgtcc tcgaccagtc ccagccgccg catgcgcgtc acgcgcagcg cgtcgctcgc
     1501 catgctcatg ctcgccgtcg tcgtcggcac cgcgttcgcc accggcgcga gcgggttccg
     1561 aggcaccccc ttggcccgcg gcaccaccgc cggacccatg cacttcaacg tcggcgacat
     1621 caagttccag accaaggcgc cggtcgactt cgctcatgcc acggtcacca tcgacccgct
     1681 cggctcctcg ggctggcatt cccacccggg cgtcgtgctc gttaccgtct ccgcggggac
     1741 ggtcaccttt tacgacgcgg attgctcgtt caacacgtac ccggtcggct cgtcgtttgt
     1801 cgagtcgaac ggcacgacgg gtctcgcgcg gaacgagagc gccagcgtca ccgccgtggt
     1861 ctacgtgacc tacatcgttc cggccggggc gcccgccctc cggatcgacc agcccaatcc
     1921 gggctgcgcg cagtcctaga cgaggccact cgcccccagt gccgttcaag gcgaggccgt
     1981 cggagcaatc cggcggcctc gctggattcc caccagacgg aaatcgaacc attcgacggc
     2041 gacgggtctc atctacggta ggcgtccagg tcggacgcat cgccgaaggg agcccgggtg
     2101 cacgttgcc
//
LOCUS       DATBSW010000116         8502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_79618, whole genome shotgun sequence.
ACCESSION   DATBSW010000116 DATBSW010000000
VERSION     DATBSW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79618"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2967)
                     /locus_tag="VIF63_03270"
     CDS             complement(<1..2967)
                     /locus_tag="VIF63_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8238433.1"
                     /translation="MSDAPQLAGSQSPSGPRARSRRPILVGGALLVVLIVVAGVLSRG
                     WLPGPVASPGPSGAVVVSDKAPLSPDGKIEIADLTHDSRSDMYRAPFGAVPAGTEVIV
                     RLRAAAGDLTEATIRVWDAVDELQALLPMTLVATDPTRGDHGYDYWQITLHTSAKPTV
                     LYYRFIVRDGTETAYLEDDPPGDGGAVAEASDGGTGRVYGASPDASWQIDVYDPAFTT
                     PDWAKGAVAYQIFPDRFFDGDPTNNPTPDAVQGTDGADVFRYGDVYGNKVLPKAWTDL
                     PEGYCRAYQGVTCNEGPLGRDFFGGDLAGVTAKLDDLAALGVTVIYLNPIFAAPSNHR
                     YDTSSYDFIDPDLGTRADFDALVQGAEARGMRVLLDGVFNHVASDSPWFDRARRFADV
                     GACEAADSPFRAWFTFRPPAANEPSPCAASTPGGDDTYYVGWFGFDTIPEVIEQSGVY
                     DIFTGPDGVVPSWIRAGTGGWRLDVMDNLSHKFMRLIRESAKAADPDALVLGEKWDDA
                     SIFLLGDQADSTMNYRFRRAVIGLVNGDTNDLDGAIAGLTPSQFRERMLGVMEDYPAP
                     AWETLLNLVDSHDTTRILWTLAPGRDDPAEKESVEGLAAAKAKVRLVSAIQLTWPGIA
                     SIYYGTEAGLTGHDDPDDRRPYPWDSIDTELRDWYRTLGQLRADHEALRTGDLQFLAA
                     DDDTGTLAYLRGTASEAAITILNLGDAAQAGVAIDVAGRIPDGTVLTDGISGASGTVA
                     AGAITVDLAAHGAAVFITPASTDLAGPDAPSDLILSSVGGAPVDIRWTAGNGAASYQV
                     WRSILSNGGYELVGTTTSTTFTDTGARSGTRQFYVIVGLDAAGNPSARSAEANILPQV
                     TLADARLAGPADVGQPLSAIDPGAPIDALVKAGPLVAGKVESASAGPTIGIRAELGVG
                     PARGGDPATDYAWTPMTWVADEADADRLRGTVRPEVLGSYNVVLRISTDGGATWAYAD
                     RGGIVAQPDGTWGYRAD"
     gene            complement(2969..4534)
                     /locus_tag="VIF63_03275"
     CDS             complement(2969..4534)
                     /locus_tag="VIF63_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEY8238434.1"
                     /translation="MTIETPDWVRDAVFYQIFPDRFAKSERVHKPGPLESWDSPPTVH
                     GFKGGDLLGVVEHLDYLADLGVTALYFNPIFQSASNHRYHTYDYMAVDPLLGGNEAFV
                     ELLDAAHRRGMRVVLDGVFNHASRGFWPFNHVMETGIASPYREWFYFDEAALEAGTPV
                     RAFPGTPPLLDTSDVPDDQRAGAKSLGIYGYRAWWDLPALPKLRIENPEVREYIFGVA
                     EHWIRLGADGWRLDVALEISDEDFWREFRRRVKAVNPEAYIVAEVWHEEPRYLQGDQF
                     DAYMNYPLTTAITSFAGAEHLDRRVLRQQFMLDEGIHAIDAPEFARRVEHVLGLYDPA
                     VVAVQLNHLGTHDTPRFLAMVGGDKAALRLAVLLQMTLPGAPSIYYGDEIGMTGEQDP
                     GSRAAFPWSDRGAWDLGILEYTTRAVAVRRAHPVLRRGGYRTIAASDASMAFIRTPAA
                     GIEGPAIVVAINAGNTEATLELDLAGLVGRLTSIPLEGPAVPPSSEVAIWDGRAAVNV
                     SPRTGLVLRVEAS"
     gene            complement(4540..5454)
                     /locus_tag="VIF63_03280"
     CDS             complement(4540..5454)
                     /locus_tag="VIF63_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8238435.1"
                     /translation="MSAPVVPAVAPVAAATRKAKATSHGLGGRTDSRGKRIVIHLLLI
                     LASIIAIFPIVRVVGVALRPGNRLLDSEFALIPPGATLQSFQHVLFETDLPLWLFNSL
                     VITVGASFVGLLLAATSAYAFARFKFPGRSVGLTLLLATQLIPAAMLLVPLYILAVQL
                     DLKNSFVGMVIAMSVTSVPFSIWILRGYFETVPIELEEAARIDGCSKLEAYWRILLPL
                     STPALAIVFLFNFLAAWNDFFLPRILISSENLLTWTLGLQRFQAQFQTQWNDLAAASI
                     FISIPVVALFLYSSKWLISGVTAGGVKG"
     gene            complement(5451..6476)
                     /locus_tag="VIF63_03285"
     CDS             complement(5451..6476)
                     /locus_tag="VIF63_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8238436.1"
                     /translation="MSWDAVRTAIEQVGLTLVGITFLVAVIEGLLYILLVRLLKWRHA
                     IPIMLVAPAAIGLAVLIVYPLVWELNVSFTKMSLRNFGDPGFLGIEKGWFVWFDNYKE
                     VFTLPVLKLTGFWQLLGQTVLWTASNVSLHVLGGLGLALLLHRKMRGKGIYRALIILP
                     WAIPQVIALLVWRTEYNFEFGAVNQLLGMVGVEPIRWLSDPFWNFVAMVITNVWLGIP
                     FMMVIILGGLQSIPDDFYEAADVDGATGRQRFRYITLPLLRPVLTPAIILGTVWTFNN
                     INVPFFINQNELETSDILVTAIFREAFQYNRYGDAAAFAFIVFILLLIFTVFYIRWTG
                     SLKGAYE"
     gene            complement(6567..7904)
                     /locus_tag="VIF63_03290"
     CDS             complement(6567..7904)
                     /locus_tag="VIF63_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8238437.1"
                     /translation="MKNPIKLAGLIGVLAIVASACAPAASTAPTTAPVTQGPAATTAP
                     PTAAAYPAGTFALTFWTKEGEGDGSLQYVKSLADAYTALHPNVTFEVVNKDVEALRED
                     FQNTGLGGDAPELLWTVGDHVGPFTAANLILPLDDLVDKASFVPAAADVVTVDGKLWG
                     APVSFGNQLMLYWNKSLAGDTAPADSAAWVAAAKTLTAGGKFGIVFNQTESFWLVPFL
                     GGFGGSVFAADGVTPTLNTPAMTGALQFLYDLKYTDKVTPAEADYNVADGLFKDGKAA
                     FIINGDWTLGAYAAPAVPTASGQAAGLGDNLGVGPLPMITGHEDPHPYIAGSFLMASK
                     AVGDDPDLKTVVSDFMKFATSKEQQIKIVQTLKRLPGNAEAIADSIVTGDPLLAGAAA
                     AAQKGVPQPTNLEMRCVFDSMTAGVRDMFTDNSDVAGIAATMQTSAETCISQL"
     gene            complement(8049..>8502)
                     /locus_tag="VIF63_03295"
     CDS             complement(8049..>8502)
                     /locus_tag="VIF63_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8238438.1"
                     /translation="LRGYRAALEAAGVNLSSERTVAGRSSIDGGSSAFQRAWAMGARP
                     TAILAMSDAMAIGAMRAARELGMRIPDDLSVVGFDDIDLAGHVDPPLTTVHQPIRQKG
                     ADAVRLLLAEVEQREANRPEHIRLETRLIVRGSTGPVPLARQEVPRAE"
BASE COUNT         1343 a         2758 c         3106 g         1295 t
ORIGIN      
        1 gtctgctcga tagccccatg tgccgtccgg ttgggcgacg atcccgccgc ggtcggcgta
       61 cgcccacgtg gcaccgccgt cggtcgagat ccgcaggacg acgttgtacg agccgagcac
      121 ctccggccgc acggtgccgc gaagccggtc ggcatcagcc tcgtcggcga cccatgtcat
      181 cggcgtccag gcgtagtccg tggctgggtc gccgccacgg gcagggccaa cgcccagctc
      241 ggccctgatg ccgatcgtcg gcccggccga cgccgactcg accttgcctg caacgagtgg
      301 gccagccttg acgagcgcgt cgatcggcgc gcccgggtcg atcgccgaga gcggctggcc
      361 gacgtccgcc ggcccagcca ggcgcgcatc ggcgagcgtg acctggggca ggatgttcgc
      421 ctccgccgac cgggcgctcg gattgcctgc ggcatcgagc ccgacgatga cgtagaactg
      481 gcgcgtgccg ctccgggcgc ccgtgtcggt gaaggtcgtg gacgtcgtcg tgccgacgag
      541 ctcgtagccg ccgttgctga ggatcgagcg ccagacctgg taggaggccg cgccgttccc
      601 ggccgtccac cggatgtcga ccggcgctcc gccgaccgac gacaggatca gatcgctcgg
      661 tgcgtccggc ccggccaggt cggtgctcgc tggggtgatg aagaccgcag cgccgtgcgc
      721 ggcgaggtcg acggtgatcg caccagcggc gacggtcccg ctggcgccgc tgatgccgtc
      781 ggtgaggacg gtcccgtcgg gaatacggcc ggccacgtcg atggcgacgc ccgcctgcgc
      841 ggcgtcaccg aggttgagga tcgtgatcgc ggcctcgctc gcggtcccgc ggaggtacgc
      901 gagcgtgccc gtgtcgtcat cggcagcgag gaactgcagg tcgcccgtcc gcagtgcctc
      961 gtggtccgct cggagctggc cgagcgtccg gtaccagtcc cggagctcgg tatcgatcga
     1021 gtcccaggga tatggccggc ggtcgtcggg atcgtcgtgg ccggtgagtc cggcctcggt
     1081 gccgtagtag atcgaggcga tgccgggcca cgtcagctgg atcgcgctca cgaggcggac
     1141 cttggccttc gcggccgcca ggccctccac agactccttc tccgcggggt cgtcgcgacc
     1201 gggcgcgagc gtccacagga tgcgcgtcgt gtcgtggctg tcgaccaggt tcagcagggt
     1261 ctcccacgcc ggcgccgggt agtcctccat gacgccaagc atccgctccc ggaactggct
     1321 cggggtgagg ccggcaatcg cgccgtcgag atcgttggtg tcgccgttga cgaggccgat
     1381 gaccgcccgc cggaagcggt agttcatcgt cgagtcggcc tggtcaccga ggaggaagat
     1441 cgacgcgtcg tcccacttct cgccgagcac cagggcgtcg gggtcggccg ccttcgccga
     1501 ctcgcggatg aggcgcatga acttgtgcga gaggttgtcc atgacgtcga ggcgccagcc
     1561 cccggtccct gcccggatcc agctcgggac gacgccatcg ggaccggtga agatgtcgta
     1621 cacgccgctc tgctcgatga cctcggggat cgtgtcgaag ccgaaccagc cgacgtaata
     1681 ggtgtcgtcg ccaccgggcg tcgaggccgc acatggcgac ggctcattcg cggcgggtgg
     1741 acggaacgtg aaccacgccc ggaaggggga gtcagccgct tcgcacgccc cgacatcggc
     1801 gaaccgccgg gctcggtcga accacggcga atccgacgcc acgtggttga agacgccgtc
     1861 gaggaggacg cgcatgccgc gcgcctctgc gccctggacg agggcgtcga agtccgcccg
     1921 cgtgccgagg tcgggatcga tgaagtcgta gctgctcgtg tcgtagcgat ggttggatgg
     1981 cgccgcgaag atcgggttga ggtagatgac cgtgacgccg agcgcggcga ggtcgtcgag
     2041 cttggcggtc acgccggcga ggtcaccgcc gaagaagtcg cgcccgagcg gcccttcgtt
     2101 gcaggtgacg ccctggtagg cgcggcaata gccctcgggc aggtcggtcc acgccttcgg
     2161 gaggaccttg ttgccgtaca cgtcgccgta tcggaagacg tcggccccgt cggtgccctg
     2221 cacggcgtcc ggcgtcggat tgttcgtcgg atcgccgtcg aagaaccggt cggggaagat
     2281 ctggtaggcg accgcaccct tcgcccagtc gggcgtcgtg aaggccgggt cgtacacgtc
     2341 gatctgccag ctggcgtccg ggctcgcgcc gtagacccgc ccagtgcccc catcgctggc
     2401 ctcggccacc gctccgccgt cgcccggcgg gtcgtcctcg aggtaggcgg tctcggtccc
     2461 gtcgcgaacg atgaaccggt agtacaggac cgtcggcttg gccgaggtgt gcagcgtgat
     2521 ctgccagtag tcgtagccgt gatcgccccg cgtcggatcc gtggcgacga gcgtcatcgg
     2581 cagcagcgcc tggagctcgt cgacggcgtc ccagacgcgg atcgtcgcct cggtcaggtc
     2641 gccggccgcg gcgcggaggc ggacgatcac ctcggtgccc gcagggacgg ccccgaacgg
     2701 ggcccgatac atgtccgagc ggctgtcatg cgtgaggtcg gcgatctcga tcttgccgtc
     2761 cgggctgagg ggagccttgt cgctcaccac gacggcgccc gacggccccg gcgacgcgac
     2821 gggtcccggc agccagccgc gagacaggac gccggcgacg acgatcagga cgaccagcag
     2881 cgcgccgccg accaggatcg ggcgccgcga ccgcgcacgt gggccactcg gcgactggct
     2941 gcccgcgagc tgcggcgcgt cgctcatctc agctcgcttc gacccggagg acgagcccgg
     3001 ttcgtggcga gacgttcacg gcggcccggc catcccagat cgcgacctcg ctgctgggcg
     3061 gcacggcggg gccctcgagc gggatcgagg tgaggcgacc cacgaggccc gcgagatcca
     3121 gctcgagcgt cgcttcggtg ttgccggcgt tgatcgcaac cacgatcgcc gggccttcga
     3181 tgcccgcggc cggcgtccgg atgaacgcca tcgaggcatc cgaggcggcg atcgtccggt
     3241 acccgccgcg gcgcaggacc ggatgggccc gacggaccgc gaccgcgcgc gtcgtgtact
     3301 cgagaatccc gaggtcccac gcgccgcgat cactccatgg gaaggcggcg cggctgccgg
     3361 ggtcctgctc gccggtcatg ccgatctcgt cgccgtaata gatcgagggt gcccccggga
     3421 gggtcatctg gagcaggacg gcgagccgga gggctgcctt gtcgccgccc accatcgcca
     3481 ggaagcgcgg cgtgtcgtgc gtgccgagat ggttcagctg gaccgccacg accgccgggt
     3541 cgtacagccc gaggacgtgc tccacgcggc gcgcgaactc gggcgcgtcg atcgcgtgga
     3601 tcccttcgtc gagcatgaac tgctggcgga ggacccgacg gtcgagatgc tcggcaccgg
     3661 cgaacgacgt gatcgcggtg gtgagcgggt agttcatgta cgcgtcgaac tggtcgccct
     3721 ggaggtagcg gggctcttca tgccagacct cggcgacgat gtacgcctcc gggttcacgg
     3781 ccttcacccg gcgccggaac tcccgccaga agtcctcgtc gctgatctcc agcgccacgt
     3841 ccaggcgcca gccatcggcg cccaggcgga tccagtgctc ggcgacgccg aagatgtact
     3901 cgcggacctc ggggttctcg atcctgagct tgggtagggc gggcaggtcc caccacgcgc
     3961 ggtagccgta gatgccgagc gacttcgcac cggcgcgctg gtcatcgggc acgtcggagg
     4021 tgtcgagcag cgggggcgtg cccggaaagg cgcgcacggg cgtgccggcc tcgagcgccg
     4081 cctcgtcgaa gtagaaccac tcgcgatacg gcgacgcgat gcccgtctcc atcacgtggt
     4141 tgaagggcca gaaaccgcgg ctcgcgtggt tgaacacgcc atcgaggacg acgcgcatcc
     4201 cacggcggtg cgccgcgtcg aggagctcga cgaaggcctc gttgccgccg agcagcgggt
     4261 cgaccgccat gtagtcgtac gtgtggtagc gatggttcga ggcggactgg aagatcgggt
     4321 tgaagtagag cgcggtgacc ccgaggtcgg cgaggtaatc gaggtgctcg acgaccccga
     4381 gcaggtcgcc acccttgaag ccatggacgg tcggtgggct gtcccacgac tcgagcggcc
     4441 ccggcttgtg cacgcgctcg ctcttcgcga accggtcggg aaagatctgg tagaagaccg
     4501 cgtctcggac ccagtcgggg gtttcgatcg tcacctcgcc tagcccttca cgccgcccgc
     4561 ggtgacgccc gagatcagcc acttcgacga gtacaggaac agggcgacga ccgggatgga
     4621 gatgaagatc gaggccgcgg cgaggtcgtt ccattgcgtc tggaactggg cctggaagcg
     4681 ctggaggccg agcgtccagg tcaggagatt ctcgctggag atcaggatcc ggggcaggaa
     4741 gaagtcgttc cacgcggcga ggaagttgaa caggaagacg atcgccagtg cgggcgtcga
     4801 gagcggcagg aggatgcgcc agtacgcctc gagcttgctg cagccgtcga tccgggcggc
     4861 ctcttcgagc tcgatcggta ccgtctcgaa gtagccccgc aggatccaga tgctgaacgg
     4921 cacggacgtg acggacatgg cgatgaccat gccgacgaac gagttcttga ggtcgagctg
     4981 gacggccagg atgtacagcg gcacgagcag catcgctgcc gggatgagct gggtcgccag
     5041 cagcagggtc aggcccacgc ttcgacccgg gaacttgaag cgcgcgaagg cgtaggcgga
     5101 ggttgcggcg agcaggaggc ccacgaacga ggcgccgacc gtgatcacga ggctgttgaa
     5161 cagccacagc gggaggtcgg tctcgaacag cacgtgctgg aacgactgga gggtggcgcc
     5221 gggcgggatc agcgcaaact cgctgtcgag gagccggttg cccggtcgta gcgcgacgcc
     5281 gacgacccgg acgatcggga agatggcgat gatcgatgcg aggatgagga gcaggtggat
     5341 gacgatccgc ttgcctcgcg agtcggtccg cccaccgagg ccgtgggacg tggccttggc
     5401 cttcctggtc gcggcggcga cgggggcaac ggcagggacg accggagcgc tcattcgtag
     5461 gcccccttca ggctgcccgt ccagcgaatg tagaagacgg taaagatgag caacaggatg
     5521 aacacgatga acgcgaaggc cgccgcatca ccgtagcggt tgtactggaa ggcctcgcgg
     5581 aagatcgcgg tcacgaggat gtcgctcgtc tccagctcgt tctggttgat gaagaagggc
     5641 acgttgatgt tgttgaaggt ccacaccgta cccaggatga tcgccggggt cagcaccggt
     5701 cgcaggagcg gcagcgtgat gtaccggaag cgctgccgcc cggtcgcgcc gtccacgtcc
     5761 gcggcctcgt agaagtcgtc ggggatgctc tgtagcccgc ccaggatgat caccatcatg
     5821 aacgggatcc cgagccagac gttcgtgatg accattgcca cgaagttcca gaacgggtcg
     5881 gacagccagc ggatgggctc cacgccgacc atcccgagga gctggttcac cgcgccgaat
     5941 tcgaagttgt attccgtccg ccagacgagc agggcgatga cctgcggaat ggcccagggc
     6001 aggatgatga gggcccggta gatgcccttg ccgcgcatct tgcgatgcag caggagggcg
     6061 agtccgagac cgccgaggac gtgcagcgag acgttgctcg ccgtccagag gacggtctgg
     6121 ccaaggagct gccaaaagcc cgtcagcttc aggaccggca gcgtgaagac ttccttgtag
     6181 ttgtcgaacc acacgaacca gcccttctcg ataccgagga acccgggatc gccgaagttc
     6241 cggaggctca tcttggtgaa cgagacgttc agctcccaga cgagcgggta gacgatgagg
     6301 acggccaggc cgattgccgc cggagcgacg agcatgatcg ggatggcgtg ccgccatttg
     6361 aggaggcgga cgaggaggat gtagagcagc ccctcgatga cggccaccag gaaggtgatc
     6421 ccgacgagcg tcagcccgac ctgttcgatc gcggtgcgga ccgcgtccca gctcacgagc
     6481 tcccctccat cacatccgga ctgtcgtggg cgtgtgtgcc ggagcagcgg ccccggggca
     6541 cgtggtgccc cggggccgcg agatcactag agctgcgaga tgcacgtctc cgcggacgtc
     6601 tgcatggtcg cggcgatgcc ggcgacgtcg ctgttgtcgg tgaacatgtc acggacgcca
     6661 gcggtcatcg agtcgaagac gcagcgcatc tccaggttgg tcggctgcgg gacgcccttc
     6721 tgggcggcgg ccgcggcgcc ggcgagcaga ggatcgccgg tgacgatgga atcggcgatg
     6781 gcctcggcgt tgccgggcag tcgcttgagg gtctggacga tcttgatctg ctgttccttg
     6841 gacgtcgcga acttcatgaa gtcgctcacg acggtcttga ggtccggatc gtcgccaacg
     6901 gccttgctgg ccatcaggaa cgagccggcg atgtacgggt gcgggtcctc gtggcccgtg
     6961 atcatcggca gcgggccgac gccgaggttg tcgcccaggc cggcagcctg accgctcgcc
     7021 gtcgggactg cgggcgccgc atacgcgccg agcgtccagt cgccgttgat gatgaaggcg
     7081 gccttgccgt ccttgaacag gccatcggcg acgttgtagt cggcctcggc cggggtcacc
     7141 ttgtcggtgt acttgaggtc atacaggaac tggagggcgc ccgtcatggc gggcgtgttc
     7201 agggtcggcg tgacgccgtc cgcagcgaac acgctgccac cgaagccacc gaggaacggc
     7261 acgagccaga aggactcggt ctggttgaag acgatgccga acttgccgcc ggcggtcagg
     7321 gtcttggcgg cagcaaccca cgcggcgctg tcggccgggg ccgtgtcgcc cgcgagggac
     7381 ttgttccagt agagcatcag ctggttgccg aaggacaccg gggcgcccca cagcttgccg
     7441 tcgacggtga cgacatcagc ggccgccgga acgaagctcg ccttgtcgac gaggtcgtcc
     7501 agggggagga tcaggttggc cgcggtgaac gggccgacgt gatcgccgac cgtccacagg
     7561 agctcggggg cgtcgccgcc gaggcccgtg ttctggaagt cctcgcggag ggcctcgacg
     7621 tccttgttga cgacctcgaa cgtgacgttc gggtggagtg cggtgtacgc gtcggccagc
     7681 gacttgacgt actgcaggct gccgtcgccc tcgccttcct tggtccagaa ggtgagcgcg
     7741 aaggtgccgg cggggtacgc cgccgctgtc ggcggcgcgg tcgttgcggc gggaccctgg
     7801 gtgaccggcg ccgtcgtcgg cgcagtgctg gcagcaggcg cgcaggcgct ggccacgatg
     7861 gccaggacgc cgatcaggcc cgcaagcttg attgggttct tcattggttc agctctctcc
     7921 tctgttgcga agggtcccaa cagtgggccc atcttcatca ttcaggccgt gggagccggc
     7981 accgtgccgg tcccggtgag cgaccacagc ggcgaggcgc acctcgccgg cagctcgcac
     8041 catcgacctc actctgcccg aggcacctcc tgccgcgcga gcggcacggg cccggtcgaa
     8101 ccgcggacga tgagccgcgt ctcgagccgg atgtgctcgg gccgattcgc ctctcgctgc
     8161 tccacctcgg cgaggaggag gcgaaccgcg tcggcgccct tctgacggat cggctggtgg
     8221 accgtcgtca ggggcgggtc gacgtggccg gcgaggtcga tgtcgtcgaa gccgacgacg
     8281 ctgaggtcgt cggggatgcg catgcccagc tcacgtgcgg cacgcatggc gccgattgcc
     8341 atcgcgtcgc tcatggccag gatggcggtc gggcgggcgc ccatcgccca tgcgcgctgg
     8401 aaggcggacg agccgccatc gatcgaggac cgaccggcga cggtgcgctc gctggagagg
     8461 ttcacgccgg cggcctccag ggccgcgcgg tagccgcgca gg
//
LOCUS       DATBSW010000117         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_714398, whole genome shotgun sequence.
ACCESSION   DATBSW010000117 DATBSW010000000
VERSION     DATBSW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714398"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..2832
                     /locus_tag="VIF63_03300"
     CDS             535..2832
                     /locus_tag="VIF63_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238439.1"
                     /translation="MDKCPAGRSVAYRERVAAADRVRPPARTDPRATAATGFGGGLRE
                     GIAIGPGHRRAGGVLLAALLAIALGLSPAAAQPALAAGTLRLEADTTYTLDPDAGRVH
                     VEIDVKATSLKPNSASFIYFYREIGFPLQPEASKIRASDGSGGITITTKKHESFIEAV
                     VRLRSPIYYQESDRFTIRYDLVGGEPRSESPTRVGKAFATFGVWSWGDVGRSTVTVKV
                     PKGFETEVAGEPLAIRTTADGETLTATPEEPDTFFSIVSAENRDAYADTRLSFEGGVE
                     IVVLAWPEDDAWQRTVGSTMREGIPELRRLIGLDWPVAHDLNVRERYTPALEGFAGIF
                     FTDDQRIDVSEDLDRFVIVHEASHAWLNESLFGERWIYEGLADEYAWQVLTAVGEDPE
                     IVPERPDVEDPGHVTLNAWRFPQVIRDQKTDDRERYGYNASFWVMHQVVAAAGLDHMR
                     AAFEAASAKTTAYIGAGAPEKVTGENDWKRFLDLVEPIDEPEPAAVEAAIRDLAVSKV
                     EARPLEGRAAARARYRDLIKAGDGWLPPWAVRKLLSGWSFIGANTYMDAAFATLQLRD
                     QVAAAAAALGLEPDGALRTAYEGAQASLAAASELATEQLSALASVADAKAKVEATPDF
                     LTQLGLIGETPNVPYEAARTAFEAGDLDGAVTSATGATALITGAAAIGQQRLIIGVVV
                     AVGVLVLLIVLVMLLRRRGQRRRLGLATANSAVAPVEGSIESLAVLDARLAPPAESTT
                     LAADPASEPPPPSASPPDDHGGPQA"
     gene            2832..>3461
                     /locus_tag="VIF63_03305"
     CDS             2832..>3461
                     /locus_tag="VIF63_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238440.1"
                     /translation="MSVRRSRPSREILSGESADVYFARADRILEREGLDPVVTMEVFT
                     RREAILCGIDEVKNLLAHVLATSDPNEVTVEALEDGDAIAPKEIVLRIAARYRAFGLY
                     ETALLGMLAQSTGWATAARECVTAAAPLPVISFGARHVHPDITDVLDYAAIVGGCVGA
                     STPAGARLAGLSPTGTMPHSLVLIFGDTVKAAEAFDRDLGPEVPRIVLVD"
BASE COUNT          504 a         1249 c         1211 g          497 t
ORIGIN      
        1 ggccaaagcg gacgtcgatc tcggtgacct gtggcggacc tgggtggccc gcccggagga
       61 cgggcagatg ctcgccgatc gcgcgatcgc tcgaggcttc tatgcccgat ccgtggcgct
      121 ggcgggggag tggcagctcc cgcctgcgat tcgccaggca atgcgcgcgt ggcgcttcga
      181 cgtcgcgcag gagctgatgc tcgccgcgga tgccgtccag gtccagcggc gtcggctcga
      241 gaccgcggcg gccgcggccg acgcgaccct gcccgaccgg ttgcgcaccg ccttcgaggg
      301 cacgggcggt gtcgccgcgg ccgccgcgga agcgtcggca gagcagacgg tcgtggaggc
      361 gattgccgcc gccgaggccg cacggccgac ggagcagggg gccggcgagc aggtgatcat
      421 cgccatcggg ctcctgttcg aagatcccgg gcttgacctc ggcgccgcga agaccggcct
      481 cgcggcgggc gatctggagg gtgcctacgg gtcggcggtc gacgcgaaga ccgcatggac
      541 aagtgccccg caggccggtc ggtcgcgtat cgtgagcgtg tcgctgctgc tgatcgcgtt
      601 cgtcctcctg ctcggactga tccgcgagcg acggcggcga ctggcttcgg cggcgggctg
      661 agggagggca tcgcgatcgg tccgggccat cgccgtgcgg gcggcgtcct gctcgccgcg
      721 ctcctcgcca tcgcccttgg cctctcgccg gcggcggccc agccggccct cgccgcgggc
      781 acgctcaggc tcgaagccga cacgacctac accctcgacc ctgacgccgg tcgggtccac
      841 gtcgagatcg acgtcaaggc gaccagcctc aagccgaact ccgcctcgtt catctacttc
      901 tatcgtgaga tcggcttccc gctgcaaccg gaggcgtcga agatccgggc gtcggacggc
      961 agcggcggaa tcacgatcac gacgaagaag cacgagtcct tcatcgaggc cgtcgtccgc
     1021 ctgcgctccc cgatctacta ccaggaaagc gatcgattca cgatccgcta cgacctggtc
     1081 ggtggcgagc cgcgctccga atcgccgacc cgcgtcggca aggcgttcgc cacgttcggc
     1141 gtgtggtcgt ggggcgacgt cggtcgcagc accgtcacgg tcaaggtgcc gaaggggttc
     1201 gagaccgagg tcgcaggcga accgctcgcg atcaggacga cggccgatgg cgagacgctc
     1261 accgcgacgc cggaggagcc ggacacgttc ttctcgatcg tcagcgcgga gaaccgagac
     1321 gcctatgcgg acacgcggct ttcgttcgaa ggcggggtcg agatcgtcgt ccttgcctgg
     1381 ccggaggacg acgcctggca gcgcacggtc gggtcgacga tgcgcgaggg catccccgag
     1441 ctccggcgcc tgatcggcct cgactggccg gttgcccacg acctcaacgt ccgcgagcgc
     1501 tacacgccgg ccctcgaggg cttcgccggg atcttcttca ccgacgacca gcggatcgac
     1561 gtcagcgagg acctcgatcg gttcgtgatc gttcacgagg cgtcgcatgc ctggctcaac
     1621 gagtcgctgt tcggcgagcg ctggatctac gagggcctcg ccgacgagta cgcgtggcag
     1681 gtgctcacgg ccgtgggcga ggaccccgag atcgtgccag agcggccgga cgtcgaggac
     1741 ccgggccacg tgaccctcaa cgcgtggcgg ttcccgcagg tcatccggga ccagaagacc
     1801 gacgatcgcg agcgctacgg ctacaacgca tcgttctggg tcatgcacca ggtcgtcgcg
     1861 gccgcgggcc tcgaccatat gcgggcggca ttcgaggcgg cctctgcgaa gacgacggcg
     1921 tacatcggcg ccggcgcgcc cgaaaaggtc acgggcgaga acgactggaa gcgattcctc
     1981 gacctcgtcg agccgatcga cgagcccgaa cccgccgccg tcgaggcggc gatccgcgac
     2041 ctcgcggtgt cgaaggtcga ggcccgaccg ctcgagggtc gagccgcggc gcgtgctcgg
     2101 tatcgcgacc tcatcaaggc gggcgacggc tggctcccgc cgtgggcggt ccgcaagctg
     2161 ctcagcggct ggtcgttcat cggcgccaac acgtacatgg atgcggcgtt cgccacgctc
     2221 cagctccgtg accaggtcgc ggccgccgcc gccgccctcg gcctcgagcc cgacggcgcg
     2281 ctgcgcaccg cctacgaggg cgcccaggcc agcctcgccg cggcgtccga gctcgcgacc
     2341 gagcagctct cggccctcgc ttccgtggcc gatgccaagg cgaaggtcga ggcgacgccg
     2401 gacttcctga cgcagctcgg gctcatcggc gagacgccga acgtgccgta cgaggcggca
     2461 cggaccgcct tcgaggcggg cgacctcgac ggcgccgtca cctcggcgac cggagcgacg
     2521 gcgctcatca ccggagcagc ggccatcggc cagcagcgcc tgatcatcgg agtggttgtc
     2581 gcagtcggcg tgctcgtgct gctgatcgtg ctggtgatgc tcctccggcg acgtgggcaa
     2641 cggcgccggc tcggactcgc caccgcaaac tccgccgtgg cgcccgtcga aggctcgatc
     2701 gagtcgctcg ccgtcctcga cgcacgactc gccccacctg cggagtcgac tacactcgcc
     2761 gccgatccgg cctcggagcc gccgccaccg agcgcaagcc cgccggacga ccatggaggc
     2821 ccacaggcct gatgtcggtc cgccggagcc gcccctctcg cgagattctc tcgggcgagt
     2881 cggccgacgt ctatttcgcg cgagccgatc gaatcctcga gcgcgagggc ctcgaccccg
     2941 tcgtgaccat ggaagtgttc acccgccgcg aggccatcct gtgcggcatc gacgaggtca
     3001 agaacctcct cgcccacgtg cttgcgacga gcgatccgaa cgaggtgacc gtcgaggcgc
     3061 tcgaggacgg tgacgcgatc gcgccgaagg agatcgttct tcgaatcgcc gcccgctacc
     3121 gagcgttcgg cctctacgag accgcgctcc tgggcatgct cgcccagtcg acgggctggg
     3181 ccaccgccgc gcgggagtgc gtgaccgccg ccgcgccgct gcccgtgatc tcgttcggcg
     3241 cccgccacgt ccacccggac atcaccgacg tgctcgacta cgcggcgatc gtcggtggct
     3301 gcgtcggcgc ctcgacccca gccggcgcgc gcctcgccgg cctgtccccg accggcacga
     3361 tgccccactc gctcgtcctg atcttcggcg atacggtgaa ggcggccgag gcgttcgacc
     3421 gcgatctcgg gcccgaggtg ccccggatcg tcctcgtcga c
//
LOCUS       DATBSW010000118         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1243789, whole genome shotgun sequence.
ACCESSION   DATBSW010000118 DATBSW010000000
VERSION     DATBSW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243789"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VIF63_03310"
     CDS             complement(<1..881)
                     /locus_tag="VIF63_03310"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY8238441.1"
                     /translation="MPARPENEPAATGRMRGRNPRRTSGGVPRLPWRRIVNPFTPLEI
                     ISADQVETIHLASLAILRDIGVEVLGARALDLFEKAGVKVDREDGARGPAGRVRFDPD
                     QLEALVALAPSRFELHARNPERGLVFGDRHVVFGAVGGPAFVTDLDRGRRAGNAADMD
                     AYVRVIGSLDIIHQEGGGPLEPTDLPVETRHLDMYRTFATLLDKPWQCLAFGSTVVDD
                     AIEVACITRGIDRSAIVDQPSLMTIVNSNSPLRLDGPMSDGLIEMATWGQPVVATPFT
                     LAGAMTPATLAGALAQK"
     gene            959..1600
                     /locus_tag="VIF63_03315"
     CDS             959..1600
                     /locus_tag="VIF63_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY8238442.1"
                     /translation="MPEPMPRTISVSGTGRVSVSPDIADLRLGVSITEPTVEKARTAN
                     ATAMTRVIDALKASGIAAADIQTTNLTLNPVYDYSTNTNPPRLTGYSLSNSVAATIRD
                     LGAVGRAIDAALEAGATSLDSVAFRVRDPSAAEREAREHAVFDARAKADVLAAAAGVS
                     ITGVAAISESGAPIPYPMLRGEMAKMAMSDAATPIETGMNEIAITVGVTYLID"
     gene            complement(1752..2810)
                     /locus_tag="VIF63_03320"
     CDS             complement(1752..2810)
                     /locus_tag="VIF63_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238443.1"
                     /translation="MRRPSSRRAVVAVLAASLAIAAPVVVVAKQATGCTTGSALVFFP
                     NPLVSSGNPNLTDQKDADYAALNDERVEVTLTGLDGSGFLSGTWATVKSSTGTPAYGG
                     ATCTFKYTRHDDRFEQVMAYYWVTRSQDYLHSLGFDGAQWPLINGDQQRVRINQYGAD
                     NSFATDHPVDEMRFGKGGVDDAEDAEVILHELGHQIHFSQSATFFATTEAGAISEGFG
                     DYWAATVSEWALDQAGMAGESDPACIMDWDSTSYTPPGPVHCLRRLDEDKRYPDDLDG
                     EVHSDGEIWSHALWNVRTALGPTKADTAILLAQFDWTGTDMPALADRIVDAVNDQYGA
                     GDALLAHQAFEERGILQP"
BASE COUNT          481 a         1045 c         1077 g          470 t
ORIGIN      
        1 ttcttctggg cgagcgcgcc ggcgagggtc gccggggtca tggctcccgc gagggtgaac
       61 ggcgtcgcga ccacgggttg tccccacgtc gccatctcga tgaggccgtc ggacatcggg
      121 ccgtcgaggc gcagcggcga gttcgagttc acgatcgtca tgaggctggg ttgatcgacg
      181 atcgccgaac ggtcgatgcc gcgggtgatg caggcgacct cgattgcgtc gtcgacgacg
      241 gtcgagccga aagcgaggca ctgccacggc ttgtcgagca gcgtggcgaa ggttcgatac
      301 atgtcgagat ggcgggtctc gaccggcagg tccgtcggct cgaggggtcc gccgccttcc
      361 tggtggatga tgtcgaggct cccgatcacg cggacgtagg cgtccatgtc ggcggcattg
      421 ccggcccgcc ggccgcgatc gagatcggtg acgaacgccg gtccgccgac cgcgccgaac
      481 acgacgtgcc gatcgccgaa cacgagcccg cgctccgggt tccgcgcgtg cagctcgaag
      541 cgcgacgggg cgagcgcgac gagcgcctcc agctggtccg ggtcgaagcg gacgcggccc
      601 gccggcccgc gggcgccatc ctcacgatcg accttgaccc cggccttctc gaacaggtcg
      661 agggcgcgcg cgccgaggac ctcgaccccg atgtcccgca ggatcgcgag ggacgccaga
      721 tggatcgttt cgacctggtc ggcagagatg atctcgagcg gcgtgaacgg gttgacgatc
      781 cttcgccacg ggagtcgggg aacgccaccg gaggtccgcc gggggttgcg cccgcgcatg
      841 cgaccagtcg cggcgggctc gttctcgggt cgggcgggca cgccgtcacg ataggcggcg
      901 tttgcgcgtg cggcacgacg cggcgaaact cccggtctcg ccacctggag cacattccat
      961 gcctgaaccg atgccccgca cgatcagcgt cagcggcacg ggccgcgtct ctgtcagccc
     1021 ggatatcgcc gacctccggc tgggcgtctc catcaccgag ccgaccgtcg agaaggcacg
     1081 caccgcgaat gccacggcga tgacccgtgt catcgacgcg ctcaaggcgt cggggatcgc
     1141 cgcggcggac atccagacga cgaacctgac gctgaatccg gtctacgact actcgaccaa
     1201 cacgaatccg ccacgcctca ccggctactc gctctcgaac tcggtggccg caaccatccg
     1261 ggatctgggc gccgtcgggc gtgcaatcga tgccgcgctc gaagcgggcg cgaccagcct
     1321 ggacagcgtc gcgttccggg tacgggaccc gtcggctgcg gagcgggagg cacgcgagca
     1381 cgccgtcttc gacgcccggg cgaaagccga cgtcctggcc gcggccgcgg gcgtgtcgat
     1441 cacgggcgtc gccgcgatca gcgagtccgg cgccccgatc ccctacccga tgctgcgcgg
     1501 tgagatggcc aagatggcca tgagcgacgc cgcgacaccc atcgagaccg ggatgaacga
     1561 gatcgcgatc acggtcgggg tgacctacct gatcgattga cgggctaggt ttcgatggcc
     1621 ccgccgcgct ctgccaggtg gcggcgcaac gtgtacgccg gatcggtcct gcgccgggcg
     1681 aggtagccgc cgacatccag ctgctcgcaa ccgccgcggc agggagcgcc atggatcgca
     1741 gctcgcccgc cctagggctg gaggattcct cgctcctcga aggcctgatg ggcgagcagc
     1801 gcatcgccgg cgccgtactg gtcgttcacc gcatcgacga tgcggtcggc caacgcgggc
     1861 atatccgtgc cggtccagtc gaactgggcc agcaggatcg ccgtgtcggc cttcgtcggg
     1921 ccgagcgccg ttcgcacgtt ccacaacgcg tgcgaccaga tctcgccgtc gctgtggacc
     1981 tcgccgtcga gatcgtcggg gtaccgcttg tcctcgtcga ggcgacggag gcaatggacc
     2041 gggcctggcg gcgtgtacga cgtcgaatcc caatccatga tgcaggccgg atccgattcg
     2101 ccggccatgc cggcctggtc gagcgcccac tcgctgacgg ttgcggccca gtagtcgccg
     2161 aagccctcgc tgatcgcgcc ggcctccgtc gtggcgaaga acgtcgctga ctggctgaag
     2221 tggatctggt gaccgagctc gtgcaggatg acctcggcgt cctcggcgtc atcgacgccg
     2281 cccttgccga agcgcatctc gtcaaccggg tggtctgtgg cgaacgagtt gtcagcgccg
     2341 tactggttga ttcggacacg ttgctggtca ccgttgatga ggggccactg cgcgccatcg
     2401 aagccgaggc tgtgcaggta gtcctgcgag cgcgtgaccc agtagtaggc catgacctgc
     2461 tcgaagcggt cgtcgtggcg ggtgtacttg aaggtgcagg tcgcgccgcc gtacgccggg
     2521 gtgcccgtcg agctcttgac cgtcgcccag gtgccgctga ggaagccgga accgtcaagg
     2581 ccggtcaggg tcacctcgac ccgctcgtcg ttcagggccg cgtagtccgc gtccttctgg
     2641 tcggtcaggt tcggattgcc gctgctcacg aggggattgg ggaagaacac cagggccgag
     2701 ccggtcgtac agccagtcgc ctgcttggcg acaacgacga cgggcgcggc aattgccagc
     2761 gacgcagcca gaaccgcaac gacggcgcga cgggacgatg ggcgacgcat gacttcctcc
     2821 aagaagcacg gggtttcggg ggcccctggg tcgggcctcg gggtcgggct ggcacggtac
     2881 gcccggctgc tcgagaccgt caaacaccca gccccgacgc ggtgcgcccg gtcaccacat
     2941 gtcgcgacga agggatacgc tcgaccgctg acagacaacc cgtcacgccc gacgtctagg
     3001 cttccatcgt gcagctgatc gacggccagc ccgtctacgc ggccacggac ctcgtcggct
     3061 acctcgcctg ctc
//
LOCUS       DATBSW010000119         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_820905, whole genome shotgun sequence.
ACCESSION   DATBSW010000119 DATBSW010000000
VERSION     DATBSW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820905"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VIF63_03325"
     CDS             <1..716
                     /locus_tag="VIF63_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8238444.1"
                     /translation="GRRSMQLAWTHLLPSTFGGAARMNVANALAAAAAAWATGAHLHD
                     IRQGLRTFTTSFFQAPGRLNYLEVGGVRVVIDYCHNVDGMRNLADFTRRMSDDGSGNG
                     TGRAGRTLGVIGMPGDRRDEDMREYGALAAAAFDEIIVREDRNLRGRAPGETATHVAD
                     GVRSVRENGANRTTRVDKILDEMSAVKTALRRANPGDLVVMCVDDSIAVYREAMASAG
                     HLAGATAFADPGELEAPEG"
     gene            complement(720..1403)
                     /locus_tag="VIF63_03330"
     CDS             complement(720..1403)
                     /locus_tag="VIF63_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY8238445.1"
                     /translation="MKLFLTSAGITNPSLQATLVELLGKPIAECGALLIPTSGYGHPN
                     TTPAHAWRFISGNEPRTPMAELGWKSLGVLELTALPSLDKDRWVQWVRESDVLLVNGG
                     DPLYLSYWMRESGLADLLPSLPDLLWVGLSAGSLVMTPRIGEDFVHWRPPTGGDETLG
                     FVDFSIFPHLDHPDLPENTMADAERWAAGMPLPCYAIDDLTGIKVVDGTVEVISEGHW
                     KLFNASATG"
     gene            1537..1959
                     /locus_tag="VIF63_03335"
     CDS             1537..1959
                     /locus_tag="VIF63_03335"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8238446.1"
                     /translation="MHFEKSIEIDASAQRVWDVLTDLEAWPERIETVDAVELLTPAPM
                     TKGSRVRLRQPKLPEGTWDVTAWDAPASFEWTQKEGGITNVAGHRVEALGDGRARLTL
                     TLDMSGLLVPIFGRLYAGLTNRYMTLEAEGMKRAAEST"
     gene            complement(1964..2869)
                     /locus_tag="VIF63_03340"
     CDS             complement(1964..2869)
                     /locus_tag="VIF63_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8238447.1"
                     /translation="MTREAFHLVSGSVPLDKAPDGNSVFLEAPQGLIVIDTGRHPEHS
                     ARLIDYARDRERPIAAIVNSHWHLDHTTGNLDIREAFPAVETYATTAIEGALVGILDR
                     HRAQTDAVLADPSTPSERKAQLLRGRHRIDHPDTLRPTRPVLRSGPISIAGRTLDVRV
                     APFAATEADLWIYDREAKLAIVGDLVVDLVPFMDTACPDGWSKALDEIEYAPFETLIP
                     GHGPVMSRADFLTWKTAFNNLVACGRSNVDTGQCVAGWEHDAAMFIPDSHRDYVREAA
                     DDYITTRLRSSEEEQQRFCKPLEAA"
     gene            complement(2901..3401)
                     /locus_tag="VIF63_03345"
     CDS             complement(2901..3401)
                     /locus_tag="VIF63_03345"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEY8238448.1"
                     /translation="MPEARDDSPLDRRRDPDDWWPAGTPRETLIDRQIHQAMAEGAFD
                     NLPHRGEPLPNDENPYASEWGLAFSVLKNAGFAPPWIEADKEVRALVARRDAIVTRAA
                     ASPTSATARRRGREQLESLVEQINAAIARVNAEAPSIRQNRQPLRLAEELRRFDEACT
                     QALDST"
     gene            complement(3409..>4438)
                     /locus_tag="VIF63_03350"
     CDS             complement(3409..>4438)
                     /locus_tag="VIF63_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY8238449.1"
                     /translation="RGGILLRGVGYESNDASVITNVSSDHMDLQGIHTLPELTEVKSV
                     IARLTKPDGWVVLNADDKYVAGIAAKVRGRVAFFTLQGDDSARVRRHLRGGGRAYLVR
                     DGVLGEAEGDAWRPFGALADIPVVLGGAARHNVANALAAAGGARAMGASLESVRKALR
                     SFVPTTEESRGRLNVFRDDKRVVIVDFAHNEAGVAVLLDVVDAIARGVGTGGRKAPIS
                     GIIGLAGDRPDDTLRGVGKQVARRADHLVQKEMLHYLRGRTRESVLGEIRAGMLEGGW
                     KDEIPVYLDEPAALAAELDRTENAERPEVIFLLCHEDREGVYRLIAERGLKPVDDPKD
                     LARLVSSR"
BASE COUNT          672 a         1509 c         1561 g          696 t
ORIGIN      
        1 acggccggcg cagcatgcag ctcgcgtgga cgcacctgct gccctcgacg ttcggcggcg
       61 cggcgcggat gaacgtcgcc aacgcgctgg ccgcggccgc ggcggcctgg gcgaccggcg
      121 cgcacctgca cgacatccgg cagggcctgc gcacgttcac gacatcgttc ttccaggcgc
      181 cgggccggct caactacctc gaggtcggcg gcgtccgcgt cgtgatcgac tactgccaca
      241 acgtcgacgg catgcgcaac ctcgccgact tcacgcggcg catgagcgac gacgggtccg
      301 gcaacggcac cggtcgcgcg ggccggacgc tgggcgtcat cggcatgccc ggcgaccgcc
      361 gcgacgagga catgcgcgag tacggcgcgc tggccgccgc cgcgttcgac gagatcatcg
      421 tccgcgagga ccgcaacctg cgcgggcgcg cgccgggcga gacggcgacc cacgttgccg
      481 atggcgtgcg gtcggtgcgg gagaacggcg ccaatcgaac gacccgcgtc gacaagatcc
      541 tcgatgagat gtcggcggtg aagacagcgc tccgtcgggc gaaccccggc gacctcgtgg
      601 tcatgtgcgt cgacgattcg attgccgtct atcgcgaggc gatggccagc gccggccacc
      661 ttgccggcgc gaccgcattc gccgaccccg gtgagctcga ggcgcccgag ggctagccgc
      721 tagcccgtcg cgctggcgtt gaacagcttc cagtgcccct cggagatgac ctcgaccgtg
      781 ccgtcgacca ccttgatccc cgtcaggtcg tcgatcgcgt agcagggcag cggcatgccg
      841 gcggcccagc gttcggcgtc cgccatcgtg ttctcgggca ggtccgggtg atccaggtgc
      901 gggaagatcg agaagtcaac gaaacccagc gtttcgtcac cgccggtggg cggcctccag
      961 tggacgaagt cctccccgat ccgaggcgtc atcaccaggc tgccggcgct caaccccacc
     1021 cacagcaggt cgggcagcga cggcagcagg tcggccagtc cggactcgcg catccagtag
     1081 gacaggtaca gcgggtcgcc gccgttcacc aggagcacgt ccgactcgcg aacccactgg
     1141 acccagcggt ccttgtcgag gctgggcagc gcggtgagct ccaggacgcc gagtgacttc
     1201 caacccagct cggccatggg cgtcctgggt tcattgccgc taatgaaccg ccaggcgtgg
     1261 gcgggcgtgg tgttcgggtg accgtacccc gacgttggaa tcagcagggc gccgcactcg
     1321 gcaatcggct tgccgaggag ctcgaccagc gtggcctgaa ggctggggtt cgtgatcccg
     1381 gcggacgtga ggaacagctt catgaggccg ctttggctcc ttcgtggggc ggggcttgtg
     1441 ggtgcaatgg ccatcctatg gaagcccacg acgttgcgcc ctgtgcgatc gcgcagggct
     1501 catggatggc acgcgcccgg ctacagtgcg ggtgccatgc attttgagaa gtccatcgag
     1561 atcgacgctt cggcccagcg cgtctgggac gtgctgaccg acctcgaggc ctggccggag
     1621 cgcatcgaga cggtcgacgc cgtcgagctg ctgacgccgg caccgatgac caaaggcagc
     1681 cgcgtccgcc tccggcaacc gaagctgccc gaggggacct gggacgtcac cgcgtgggac
     1741 gcacccgcct ccttcgaatg gacgcagaag gagggcggca tcaccaacgt ggccgggcat
     1801 cgggtcgagg cgttgggtga cggccgcgca cggctcacgc tgaccctgga catgagcggc
     1861 ctgctcgtcc cgatctttgg ccggctctac gcggggctga ccaaccgcta catgaccctc
     1921 gaagccgagg gcatgaagcg agccgccgaa tcgacctagc cgctcaggcg gcctcgagtg
     1981 gcttgcagaa tctctgctgc tcctcttcgg atgaacgcag ccgcgtcgtg atgtagtcgt
     2041 cggcggcttc cctgacgtag tcgcggtggc tgtcgggaat gaacatcgcc gcgtcgtgct
     2101 cccagccggc gacgcactgc cccgtgtcga cgttcgacct gccgcaggca acgagattgt
     2161 tgaatgcggt cttccaggtg aggaagtcgg cccggctcat caccggccca tgccccggga
     2221 tgagggtttc gaacggcgcg tattcgatct cgtcgagcgc cttgctccac ccgtccgggc
     2281 aggcagtgtc catgaagggc acgaggtcga ccaccaggtc accgacgatc gcgagcttcg
     2341 cttcgcggtc gtagatccac aggtcggcct cagtcgcggc gaacggcgcg acccggacgt
     2401 cgagcgtccg gcccgcgatg ctgatcggcc ctgagcgcag gaccggtcgc gtcggccgca
     2461 gggtgtcggg atgatcgatg cggtgtcggc cgcgcagcag ctgcgccttc cgctccgacg
     2521 gggtgctcgg gtctgccaac accgcatccg tttgcgcacg gtggcggtcg aggatgccga
     2581 ccagggcgcc ctcgatcgcg gtcgtggcat aggtctcgac ggccggaaac gcttcgcgga
     2641 tgtcgagatt gcccgtcgtg tggtcgagat gccagtggct gttgacgatg gcggcgatcg
     2701 gacgctcccg gtcgcgggcg tagtcgatca acctggccga atgctcggga tgacggcccg
     2761 tgtcgatcac gatcaggccc tgtggcgcct cgaggaacac gctgttgccg tccggcgcct
     2821 tgtcgagcgg caccgagccc gaaacgaggt ggaatgcctc gcgtgtcata agcgctcgga
     2881 tgagttggcg tcgcccaacg tcacgtggag tcgagagcct gcgtgcacgc ctcatcgaat
     2941 cgacgaagct cctcggcgag acgcagcggc tggcgattct ggcgaatgct cggcgcctcg
     3001 gcgttgactc gcgcaattgc cgcattgatc tgctcgacca gcgattcgag ctgttcgcgg
     3061 ccacgccgac gcgcagtggc cgacgtcggc gaggccgcgg cacgcgtgac aatggcgtcg
     3121 cgccgagcga cgagtgcgcg gacctccttg tcggcctcga tccagggtgg cgcaaagccg
     3181 gcgttcttga gcacgctgaa ggcgagaccc cattcggagg cgtacggatt ctcgtcgttg
     3241 ggcaacggct cgccccggtg cggcaagttg tcgaacgcac cctccgccat ggcctgatgg
     3301 atctgccggt cgatcagggt ctcccgtgga gtcccggctg gccaccagtc atccgggtcg
     3361 cgccggcgat cgagcggcga gtcatcacgt gcttccggca tggttcggtc agcgtgacga
     3421 cacgaggcgg gcaaggtcct ttgggtcgtc gactggtttg aggccgcgtt cggcgatgag
     3481 gcggtagacg ccctcgcggt cctcgtggca gagcaggaag atcacctcgg ggcgttcggc
     3541 gttctcggtg cggtcgagct cggcggcgag ggccgcgggc tcgtcgaggt agaccgggat
     3601 ctcgtccttc cagccgccct cgagcatgcc ggcgcggatc tcgccgagca cggactcccg
     3661 cgtccggccg cgaaggtagt ggagcatctc cttctggacg aggtgatccg cgcgccgggc
     3721 cacctgcttg cccacgccac ggagcgtgtc gtcgggtcga tcaccggcca ggccgatgat
     3781 cccgctgatc ggcgcctttc gaccgccggt gccgacgcct cgcgcgatcg catcgacgac
     3841 gtcgaggagc accgccacgc cggcctcgtt gtgggcgaaa tcgacgatga cgacccgctt
     3901 gtcatcgcgg aagacgttga ggcgcccgcg ggactcctcg gtcgtcggca cgaacgagcg
     3961 gagggccttg cgcacgctct cgagcgaggc gcccatcgcc cgcgcgccgc cggctgccgc
     4021 gagggcgttc gcgacgttgt ggcgagccgc gcccccgagc acgacgggaa tgtccgccag
     4081 cgccccgaac gggcgccatg cgtcgccctc ggcctcaccg agcaccccgt cacgcaccag
     4141 gtacgcacgc ccgccgccgc ggaggtggcg tcgaacccgc gccgagtcgt cgccctggag
     4201 cgtgaagaac gccacgcgcc cgcgcacctt ggcggcgatc ccggcgacgt acttgtcgtc
     4261 ggcgttcagc acgacccagc cgtcgggctt cgtgagccgg gcgatcaccg acttcacctc
     4321 ggtgagctcg ggcagcgtgt ggatcccctg gaggtccatg tggtccgacg agacgttggt
     4381 gatgacgctc gcgtcgttcg actcatagcc gaccccgcgc aggaggatgc cgccgcgg
//
LOCUS       DATBSW010000120         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_186187, whole genome shotgun sequence.
ACCESSION   DATBSW010000120 DATBSW010000000
VERSION     DATBSW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186187"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..944
                     /locus_tag="VIF63_03355"
     CDS             438..944
                     /locus_tag="VIF63_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238450.1"
                     /translation="MAAETFVDIAYRGIELGRRHRLCEVGPRTAYVEHPTPMPVGSRL
                     ELLADGGVALAVEVVRIHEQVAGATRAPGMRVQAPALDERGASLWRTLVSVADPVVAP
                     VVVEDVDTTVVDGEVFAGGADRRPTEVMAAIDVEAITRQAAADKPDDGDRPEPAGKKK
                     RGGRKKKS"
     gene            complement(941..1903)
                     /locus_tag="VIF63_03360"
     CDS             complement(941..1903)
                     /locus_tag="VIF63_03360"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY8238451.1"
                     /translation="MNIHKLARTTPASRALIASRRAAGLSEGVIAAQFGVDRKTVRKW
                     SRRQQAEGERGLRDRTSRPSTMPSALDAAWLDAVLALRRLKFTQQQVALVLGLSKSRV
                     QRVVAGRGLGKLSALEPPRPANRYERTRPGELVHVDTKKLGRFFQPGHRVTGDRAGSN
                     RLRGHKGWEFLHIAIDDRTRLAFAELLSDEKAPTTAAFLLRAARFFKRHGIARIERVM
                     SDNGGAYRSDDFRTAVDLIQAKHLRTRPYTPRTNGKAERFIQTMLRLWAYVRPYGSSD
                     ERATTLAPWLRWYNQLRPHGSLDGRSPMDTLKQLRRDNVLGDHT"
     gene            complement(2009..>2534)
                     /locus_tag="VIF63_03365"
     CDS             complement(2009..>2534)
                     /locus_tag="VIF63_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY8238452.1"
                     /translation="GVAACMQALTLLRRLGLAPRRTIRVVLWTNEEFGLRGAHHYAAN
                     HAEELPHHVLAVEMDGGGFAPRGFSFEGADDATLARAADLATLLAPIGAGGVHAGHSG
                     ADVGALVERGVLGAGLEVDPTRYFDYHHTEADTLDKVDPADLALDVAALAVLAYVVAD
                     LPDRLGGPAAPAAP"
BASE COUNT          366 a          838 c          968 g          362 t
ORIGIN      
        1 cgtcggccgg ccagcaaccg tcgcggtcgc actcgtagca gccggtgtcg tcgcagtagt
       61 agtcctcctg gtcgtcgtgg atgtagatcg gacatccggc gaggccgagg gggacggcga
      121 cgagcacgag cgcggcgaac aggcgatggg acatggaggg ctccttcggt tcggacgagg
      181 cgaggctgag cgcccggtgt gcaccgcgtg tgccacgcgc gcccggcgcg gcaagcgagc
      241 gtcggcgcag gcagatccgg gcacacccac ggcgatctcc ggaacccgcg cgccggcgtg
      301 tagtcggtcg ttcacgcgcg gatgtggcgg gaggttcacg ccggcatggc agacgtgtcc
      361 gaggtccacc gcgacgacgg tgagctcccg acggtccgcg cacctcgacc tacacgtccg
      421 cgtactacga tggcgcgatg gcggccgaga ccttcgtcga catcgcgtac cgcggcatcg
      481 agctcggccg gcgccaccgg ctgtgcgagg tcggcccgag gacggcgtac gtcgagcacc
      541 cgacgccgat gccggtcggg agccggctcg agctgctcgc cgacggtggc gtcgcgctgg
      601 ccgtcgaggt cgtccggatc cacgagcagg tcgccggcgc gacccgcgcc ccggggatgc
      661 gggtccaggc gccggcgctc gacgagcgcg gcgcgtcgct ctggcggacc ctcgtgagcg
      721 tcgccgaccc ggtcgtcgcg ccggtcgtcg tcgaggacgt cgacacgacg gtcgtcgacg
      781 gcgaggtctt cgccggcggc gccgatcgcc ggccgaccga ggtcatggcg gcgatcgacg
      841 tcgaggcgat cacgcggcag gccgcggccg acaagcccga cgacggcgac cggcccgagc
      901 cggcgggcaa gaagaagcgc ggcgggcgga agaagaagag ctaggtgtga tctcccagga
      961 cgttgtcccg tcgtagctgt ttgagggtgt ccatgggact tcttccgtcg agcgagccgt
     1021 gaggacgtag ctggttgtac cagcggagcc atggcgcgag cgtcgtggct cgttcgtcgg
     1081 atgagccgta gggccggacg taggcccaga ggcggagcat cgtctggatg aatcgctcgg
     1141 cctttccatt ggtccgcggc gtgtagggcc gggtccttag gtgcttggcc tggatgaggt
     1201 cgacggcggt tcggaagtca tcggagcggt aggcgccgcc gttgtcgctc atcacgcgct
     1261 cgatacgggc gatgccgtgg cgcttgaaga aacgagcggc acgcagcaga aacgccgcgg
     1321 tcgtcggcgc cttctcgtcg gaaagcagct cggcgaaggc cagtcgggtt cggtcatcga
     1381 tcgcaatgtg gagaaactcc cagcccttgt gaccgcgaag gcgattgctg ccggcgcggt
     1441 cgcccgtgac gcggtggccc ggctggaaga agcggccgag cttcttggtg tcgacgtgga
     1501 cgagctcgcc aggccgagtg cgttcatacc ggttcgccgg acgtggtggc tcgagcgcgc
     1561 tcagcttccc cagtccgcgg ccggcgacga cgcgctgcac gcgtgacttc gagaggccga
     1621 gcacgagggc gacctgctgc tgcgtgaact tcagccgccg aagtgccagg accgcgtcga
     1681 gccacgcagc atccaacgcc gagggcatgg tcgacggccg cgaggtccgg tcgcgaaggc
     1741 cgcgctcacc ctcggcctgc tgccgccggc tccacttccg aacggtcttg cggtccacgc
     1801 cgaattgcgc ggcgatcacc ccctccgaaa gtcccgccgc tcggcgcgac gcaatcaacg
     1861 ctcgacttgc gggcgtcgtc cgggccaact tgtggatgtt catccgcgct tcctccgagg
     1921 tgctttgggt gtttggcgac ttccagaaca ccccgcggag tgcggatgaa caactacttt
     1981 ttggggacga cctgctggga catcacagct agggcgcggc cggcgccgcc gggccgccga
     2041 ggcggtccgg caggtcggcg acgacgtagg cgagcaccgc gagcgcggcg acgtcgagcg
     2101 cgaggtcggc cgggtcgacc ttgtcgaggg tgtcggcctc ggtgtggtgg tagtcgaagt
     2161 agcgggtcgg gtcgacctcg aggccggcgc cgagcacgcc gcgctcgacc agcgcgccga
     2221 cgtcggcgcc cgagtggccg gcgtggaccc cgccggcgcc gatcggcgcg agcagcgtgg
     2281 cgagatcggc ggcgcgggcc agcgtagcgt cgtcggcgcc ctcgaacgag aagccgcgcg
     2341 gcgcgaaccc accgccgtcc atctcgaccg cgaggacgtg gtggggcagc tcctccgcgt
     2401 ggttggcggc gtagtggtgc gcgccgcgca ggccgaactc ctcgttggtc cagagcacga
     2461 cgcggatcgt ccggcggggc gcgaggccga gccggcggag cagggtgagc gcctgcatgc
     2521 acgccgcgac gccg
//
LOCUS       DATBSW010000121         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_609071, whole genome shotgun sequence.
ACCESSION   DATBSW010000121 DATBSW010000000
VERSION     DATBSW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609071"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VIF63_03370"
     CDS             <1..225
                     /locus_tag="VIF63_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8238453.1"
                     /translation="VLGLDDRIGSLEAGKEADLIAIDPRLTAPRRDLPYDARRVEADE
                     VMSRLIFRNHPDMVRGAWVRGRRLKGPPEA"
     gene            complement(229..432)
                     /locus_tag="VIF63_03375"
     CDS             complement(229..432)
                     /locus_tag="VIF63_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238454.1"
                     /translation="MNGRPLSLSRPANRTGEPEPLRHVIDQYKHREQTITCVCGWHGS
                     CATNSTGGSDWTTHLNQFRTKRK"
     gene            complement(581..1975)
                     /locus_tag="VIF63_03380"
     CDS             complement(581..1975)
                     /locus_tag="VIF63_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8238455.1"
                     /translation="MTLATDATLTAFLDDLRAALPDLRLLTEVSDRESYRHDETAYLD
                     AGLPGAVAFPVDTAEVSTILRLASQHRVAVVPRGAGTGLSGGAAGIDGAVTIVLTRMN
                     RIIEIDPDNLCVVTQPGVINAELKAAVAREGLFYPPDPASYETCSIGGNLGTNAGGLC
                     CVKYGQTRDWVLGLEVVLADGTVIRTGGRTVKDTAGYSLTHLFVGSQGTLGIITEATL
                     RLRPLPGPRATLIAFFATLGAAGDAVASMTRAGLVPVTLELLDQETILAVDDAHNLGL
                     DRAAAAMLLVESDAPGAAAGAELDEAEAAILAAGATSAVRAADAQEADWLRQARRLAL
                     RALERKGTVRMEDVGVPRSRVPELLVAIERITTAHKVRCATFGHAGDGNLHPNLIFDR
                     DDPDAARLTHVVRDEIFQAALDLGGTVTAEHGIGAARREWLPIQRGEGAVAAMRAIKG
                     ALDPLGILNPGRVL"
     gene            2017..>2667
                     /locus_tag="VIF63_03385"
     CDS             2017..>2667
                     /locus_tag="VIF63_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238456.1"
                     /translation="MSSPRNRSTFALDVPRSVTALLVLALVVVVVFVAFMPGSGGVPA
                     ATQAVAGATDAPFTDAPIASDAPIASPEATASPDSTPGPLPSREPEATDGPGSSAIVP
                     GEVNRSSLDVRATYRVNAAITVRNGKLDVSTRIVATNQSGDGIDRLELNTIAARLGGI
                     EVYEASVDGIDVTVRVDDQTLVVPLGGVLPDGATTTVLIAYRAQLRSGLTESDWMFS"
BASE COUNT          382 a          946 c          948 g          391 t
ORIGIN      
        1 gtgctcggtc tcgatgaccg gatcggctcg ctcgaggcgg gcaaggaggc cgacctgatc
       61 gcgatcgacc cgcgcctgac cgccccgcga cgcgacctgc cctacgacgc ccgacgcgtc
      121 gaagccgacg aggtcatgag ccgcttgatc ttccggaacc acccggacat ggtccgcggc
      181 gcctgggtcc gcggccgccg cctcaagggc cccccagagg cctgagcgct acttccgctt
      241 ggtgcggaac tggttcaggt gtgtcgtcca gtcggagccg ccggtcgagt tcgtggcgca
      301 gcttccgtgc cagccgcaga cgcaggtgat ggtctgctcg cggtgcttgt actggtcgat
      361 gacgtgacgc aggggctcgg gctcaccggt gcgattcgcg gggcgggaca gggacaacgg
      421 gcgtccgttc aaaggtgagc tcctgggcgc atcgccacat ggtgaccgcg tcgggggtac
      481 gacgccgggg ccgctcagta tcgccgattc ggccgattcg cgcttgccgg aggcgcgccg
      541 gcgatcgacg gcgatcgacg ccgcgcggcg ccactcgtac ctagagcacg cgccccgggt
      601 tcaggatccc gagcggatcg agcgcgccct tgatcgcgcg catcgccgcg acagcgccct
      661 cgccgcgctg gatgggcagc cactcgcgcc gtgccgcgcc gatgccgtgc tcggccgtga
      721 ccgtcccgcc gagatcgagc gcggcctgga agatctcgtc gcgaacgacg tgggtgaggc
      781 gagctgcgtc ggggtcgtcg cggtcgaaga tgagattcgg gtggaggttg ccgtcgccgg
      841 cgtggccgaa cgtcgcgcac cggaccttgt gggcagtcgt gatccgctcg atcgcgacga
      901 gcagctccgg gacccgcgag cgcgggacgc cgacgtcctc catccgcacg gtgcccttcc
      961 gctcgagggc gcgcagggcg aggcgccgcg cctggcgcag ccagtcggcc tcctgggcgt
     1021 cggcagcacg cacggccgag gtcgcacccg cagcgagaat cgccgcttcc gcctcgtcca
     1081 gctccgcacc ggccgctgcc cccggcgcgt cggattcgac gaggagcatc gccgcagccg
     1141 ctcgatcgag ccccaggttg tgggcgtcgt cgaccgcgag gatcgtctcc tggtcgagca
     1201 gctcgagggt caccggcacg aggccggcac gggtcatcga agcgaccgcg tcgccggccg
     1261 cgccgagggt tgcgaagaac gcgatgagcg ttgcccgcgg gccgggcagc gggcgcagtc
     1321 gcagcgttgc ctcggtgatg atcccgagcg tgccctgcga gccgacgaac aggtgggtca
     1381 gcgagtagcc ggccgtgtcc ttgaccgttc gaccgccggt gcggatgacc gttccgtcgg
     1441 cgaggacgac ctcgagcccg agcacccagt cacgcgtctg gccgtacttc acgcagcaca
     1501 ggccgccggc attcgtgccc aggttgccgc cgatcgagca cgtctcgtag ctcgccgggt
     1561 cgggcgggta gaacaggcct tcgcgagcga cggcggcctt cagctcggcg ttgatcacgc
     1621 ccggctgcgt gacgacacag aggttgtcgg ggtcaatctc gatgatccgg ttcatgcgcg
     1681 tcagcacgat cgtgacggct ccgtcgatgc ccgccgcacc gcccgacagg cccgtgccgg
     1741 cgccacgcgg cacgacggcg acgcggtgct gcgaagcgag ccggaggatc gtcgaaacct
     1801 cggcggtgtc gaccgggaac gcgaccgcgc cgggcaggcc ggcgtcgagg tacgcggtct
     1861 cgtcgtggcg gtatgactcg cgatcgctga cctcggtcag gagtcgcagg tcgggcagcg
     1921 cggcccgcag gtcgtcgagg aacgcagtca acgtcgcatc ggtggcgagg gtcaccaccg
     1981 aatcgtagcg gtcgggcgtc gtctacgatc cgcgccgtga gctcaccgcg gaatcgctcc
     2041 acgttcgcgc tcgacgtgcc gaggagcgtc accgcgctgc tggtcctcgc cctcgtcgtg
     2101 gtcgtggtgt tcgtcgcctt catgccgggt tccggcggcg tcccggcggc aacccaggcc
     2161 gtggccggcg ccaccgacgc acccttcacc gacgcaccga tcgcctccga cgctccaatt
     2221 gcatcgcccg aagcaactgc ctcgcccgat tcgacgcccg gtcccttgcc gtcgcgcgag
     2281 cccgaggcaa cggacggccc aggctcgagt gcgatcgtgc ccggcgaggt caatcgatcc
     2341 agcctcgacg tgcgcgcgac ctaccgcgtc aatgccgcga tcacggtgcg caacggcaag
     2401 ctcgacgtct cgacacggat cgtcgccacg aatcagtcgg gcgacggaat cgaccggctc
     2461 gagctgaaca ccatcgccgc caggctcggt ggcatcgagg tctacgaggc cagcgtcgac
     2521 ggcatcgacg tcacggtcag ggtcgacgac cagaccctcg tcgtgcccct cggcggcgtg
     2581 ctcccggacg gcgccacgac cacggtgctc atcgcctacc gggcgcagct gcgcagtggc
     2641 ctaaccgagt cggactggat gttctcg
//
LOCUS       DATBSW010000122         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_503763, whole genome shotgun sequence.
ACCESSION   DATBSW010000122 DATBSW010000000
VERSION     DATBSW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503763"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VIF63_03390"
     CDS             <1..643
                     /locus_tag="VIF63_03390"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY8238457.1"
                     /translation="KGEIEKLRQVRAITDLAQLAYVRQKEQLGLGHAILMAKDLIGHE
                     PFAVILPDDVVVADRPCIGQLIHAYEQTHSSVVAVTEVPAEDVSRYGIVAAEIDETHK
                     NGGRLQRMTRIVEKPAPKDAPSTLAVIGRYVLTPKIFDKLEQTQRGAGGEIQLTDAIE
                     ALMDEQSVYTYQFEGVRYDAGTTLGWLQATVELALQRPDLAADFRKHLRGLDL"
     gene            640..2622
                     /gene="pknB"
                     /locus_tag="VIF63_03395"
     CDS             640..2622
                     /gene="pknB"
                     /locus_tag="VIF63_03395"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY8238458.1"
                     /translation="MTDIGTVLGGRYRLIELLGQGGMATIYRARDAQLERDVAVKVLR
                     PEYGADPDFIDRFRHEAQSAASLNHPSVVAVYDYGTDPVGPFIVMELVEGEDLSAIIR
                     RSGALPPRAAARLIAQAARAIAAAHASGFVHRDVKPGNILVTREGRVKVADFGIARAL
                     SESALTLPGTTLGSVHYFSPEQARGELATPASDIYSLGIVLFELLTGRRPWTGDSAAA
                     IATARLTGAVPSPAAVHGGIPPTLEAIDRKALATNPEDRFASAADMAAALEQFLGEER
                     GIGGGAATGAAAGAGAAGMSGAAGAAGVGAMAGAAAAGAAAARAAAVGGAASPPTVAA
                     GVARPNPEARIEYPRDAYAAGGGTLRPTRTTSAVSAGDGGDDDGDEPRGTSAWLWVAG
                     VLAIVILGLAAFALTKLIGSAGATPAPQVLVPSLLGKTFEQAQQAVTAIGLEVRIVGF
                     EENDTQLAGTITSQDPTEGATVDQGSTISVKTAISPQGVAVPDLRGRVEQDALNLITA
                     AGLTIGTRTEAFDPFFPIGSIVDHDPGPNEVVLPGTAVSYVVSKGPEVTPSPEPTPVP
                     TPVPTPVPTPVPTPEPTPEPTPVPDLTVGEYRCTLLILAKGEIENDGFTVGSVSEPNG
                     DDSIVVAQDPAPGAKRPPGSPIDLTVVSAPAPTCPT"
     gene            complement(2661..2786)
                     /locus_tag="VIF63_03400"
     CDS             complement(2661..2786)
                     /locus_tag="VIF63_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238459.1"
                     /translation="MLETVLPGASVPETPASVVFLVAEPVDAWESRTNLFGDPER"
     gene            complement(3278..4471)
                     /locus_tag="VIF63_03405"
     CDS             complement(3278..4471)
                     /locus_tag="VIF63_03405"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238460.1"
                     /translation="MPSRRASSHVRPRPPSSGRTTQAVKVAAPDRRRVRQHRGLEGRR
                     KRAPLATRTLLAVAVAVIGGVAFMTASGGLGPILTTLGAGFSTAFDRLTATPLPSSTQ
                     LPPTDSPRIAAPAQPFTNVALITLSVSVPIEVVGDPTAKVRIYLALEGLEPAPIVDVP
                     VSETSRMSVPFELTKGKNNISATLFRGDEESEQSPIVTWILDTDPPKISVTKPKNEAA
                     IDAREITIEGTTQANTTLVALNAANDTSISSMSAKDGKFAFGMLLEPGTNVITITGTD
                     PAGNVGEGKVTLIQGSTEMGVRLTASKYRISIANPPPSIQLAVLVTDPLGVPLAGAAA
                     SFTLQIPGLAPISNSLVTGPDGRAVFTTPLVGELTTGGGLGIVLVTTDLYGSSSDRVT
                     LTFVK"
     gene            4652..5074
                     /locus_tag="VIF63_03410"
     CDS             4652..5074
                     /locus_tag="VIF63_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238461.1"
                     /translation="MTGRPPFGIGPISRVSTVPTPGADSAPDRFVAGGATRPAEQRYG
                     INIGGGPARFGADRTRAFGLPNIRRNVCPHFYVTGSRGALPIHAPHLLKGIVRQDSVV
                     SRCQLRGGVHLEQSYVNDVCLGPRFNQCVFYEEELSAR"
     gene            5071..>5399
                     /locus_tag="VIF63_03415"
     CDS             5071..>5399
                     /locus_tag="VIF63_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238462.1"
                     /translation="MSRSGSPAADASAYHAAVQALAERGRFGVRLGLARTRALLRALG
                     DPQLELRGALIGGTNGKGSVLALASSALRAAGIRTGATPKPHLVSYRERLEIDGRPID
                     PVTFTRLV"
BASE COUNT          765 a         1947 c         1914 g          773 t
ORIGIN      
        1 gaagggcgag atcgagaagc tccgccaggt gcgggcgatc accgacctcg cccagctcgc
       61 ctacgtgcgc cagaaggagc agctcggcct gggccacgcg atcctgatgg ccaaggacct
      121 catcggccat gagccgttcg ccgtgatcct gcccgacgac gtcgtcgtcg ccgaccggcc
      181 gtgcatcggc cagctcatcc atgcctacga gcagacccac tcgtccgtgg tcgccgtcac
      241 cgaggtgccg gccgaggacg tgtcgcgcta cggcatcgtc gcggccgaga tcgacgagac
      301 gcacaagaac ggcggacgcc tccagcggat gacccgcatc gtcgagaagc cggcaccgaa
      361 ggacgcgccc tcgacgctcg cggtgatcgg gcggtacgtc ctgacgccca agatcttcga
      421 caagctcgag cagacccagc gcggcgccgg cggcgagatc cagctcaccg acgcgatcga
      481 ggcgctgatg gacgagcagt ccgtgtacac gtaccagttc gagggcgtgc gctacgacgc
      541 cggcacgacc ctcggctggc tccaggccac cgtcgagctg gcgctgcagc gcccggacct
      601 cgcagccgac ttccgcaagc acctgcgcgg gctcgacctt tgaccgacat cgggacggtc
      661 ctcggcggcc gctaccggct catcgagctg ctcggccagg gcggcatggc cacgatctac
      721 cgggcccgcg acgcccagct cgagcgcgac gtcgcggtca aggtgctccg ccccgagtac
      781 ggcgccgacc ccgatttcat cgatcgcttc cggcacgagg cgcagtcggc cgcgtcgctc
      841 aaccacccat cggtcgtcgc ggtgtacgac tacggcaccg atccggtcgg gccgttcatc
      901 gtcatggagc tggtcgaggg cgaggacctc tcggcgatca tcaggcgaag cggcgcgctg
      961 ccgccgcgcg cagcggcgag gctcatcgca caggccgcgc gggccatcgc tgccgcgcac
     1021 gccagcggct tcgtccatcg cgacgtcaag cccggcaaca tcctcgtgac ccgcgagggc
     1081 cgggtgaagg tcgccgactt cgggatcgcc cgggcgctct cggaatcggc gctgacgctg
     1141 cccggcacca cgctcggctc ggtgcactac ttcagcccgg agcaggctcg cggcgagctg
     1201 gccacgccgg cctctgacat ctacagcctc ggcatcgtcc tgttcgagtt gctcaccggc
     1261 cgccgtccgt ggacgggcga cagcgcggcg gcgatcgcca ccgctcgcct caccggcgcg
     1321 gtcccgagtc cggcggcggt ccacggcggc atcccgccga cgctcgaggc aattgaccgc
     1381 aaggcgctgg ccacgaaccc ggaagaccgt ttcgcctcgg ccgcggacat ggccgcggcg
     1441 ctcgagcagt tcctcggcga ggagcgagga atcggcggcg gagccgccac cggcgccgcg
     1501 gctggtgccg gtgctgccgg gatgtccggc gccgccggag cggctggcgt cggcgccatg
     1561 gccggggccg ccgcggccgg ggccgcagcc gccagggccg cagccgtggg cggcgcggca
     1621 tcgccgccaa ccgtggccgc gggcgtcgcc cggccgaacc cggaggcgcg gatcgagtat
     1681 ccccgggacg cctacgccgc cggcggcgga acgctgcggc caacgcggac gaccagtgcc
     1741 gtgtcggccg gtgatggtgg cgacgacgac ggcgacgagc cgcgcggcac gtcggcctgg
     1801 ctgtgggtgg caggggtgct ggcgatcgtc atcctcggcc tcgctgcgtt tgcgctcacc
     1861 aagctcatcg gcagcgccgg cgccaccccg gcaccccagg tgctcgtacc cagcctgctc
     1921 ggcaagacct tcgagcaggc ccagcaggcc gtgactgcga tcggcctcga ggtgcggatc
     1981 gtcggcttcg aggagaacga cacgcagctc gcgggaacga tcacgtcgca ggatcccacc
     2041 gagggcgcga cggtcgacca gggctccacc atctcggtga agaccgccat ctcgccgcag
     2101 ggcgtagccg tccccgacct ccgcggcagg gtggagcagg acgccctcaa cctgatcacg
     2161 gcggcagggt tgaccatcgg cacgcgcacg gaggcattcg atccgttctt cccgatcggc
     2221 tcgatcgtcg accacgatcc cggcccgaac gaggtcgtcc tgcccggcac ggccgtgagc
     2281 tacgtcgttt cgaagggccc cgaggtgacg ccttcgccgg agccgactcc cgtcccgacc
     2341 ccggtgccga cgcccgttcc aacaccggtc ccgacgccag agccgacgcc cgaaccgacc
     2401 ccggtccccg acctgacggt cggcgagtat cgctgcacgc tgctcatcct cgccaagggc
     2461 gagatcgaga acgacggctt caccgttggt tccgtatcgg agccgaacgg cgacgactcg
     2521 atcgtcgttg cgcaggaccc ggcacctggc gccaagcgcc cgccggggtc accgatcgac
     2581 ctaactgtcg tctccgcacc ggcgcccacc tgccccacct gatcgttcgg ggtcggcgtt
     2641 ccgacgtcag cgccccgacg tcagcgctct ggatcgccga acaggttcgt ccgcgattcc
     2701 cacgcgtcga ccggctcggc aacgaggaac accacgctgg cgggcgtttc cggcactgac
     2761 gcgcccggca gcaccgtctc cagcacggtt gggacgacga tcggttcgcg ccgaggcgca
     2821 acgtcctcca ccggcacgaa cccgcgcgcc gcgaccggct cgggcgaagg cgcgcgagat
     2881 cgggaccctg ccgcattcgc cgcgttggcc ccattcggcg gctgggacca gcagccgcgc
     2941 agctcgcggc ccgtgagcgg gagccgcttt cggtcgagcg cgcagtagcc gagatcggtc
     3001 gccagcgagg cgcgaaagtg gcggcaagtc gaacaagtgg tcgacgaacg gcggcagacc
     3061 cagcaccgcg ccgtctctgc ctgtggtgcg ccgcagtgcg ggcaatgcca catcttgttg
     3121 ccgatggtag ctgcgcacgg tgtgatacgc cacgctcatt cgcatcgatc gcttcgttgt
     3181 cggctgcgcg cgctcgctca cgcaccttgg ggtgcgctcg ctcacgtgct cacctccgcc
     3241 ttgcgctcga cgcacctgag cgcggcgtga gtgattccta cttgacgaag gtcagcgtga
     3301 cccggtcgct ggaggagccg tagaggtcgg tcgtcacgag cacgatgccg agaccgccgc
     3361 cggtcgtcag ctcgcccacg agcggcgtcg tgaagacggc ccggccgtcg ggcccggtca
     3421 cgagcgagtt ggagatcggg gcgaggcccg gaatctggag cgtgaacgac gccgctgcgc
     3481 cggcgagggg cacgccgagc gggtcggtga cgagaacggc cagctggatc gacggcgggg
     3541 gattcgcgat cgagatccgg tacttggacg cggtcagccg gacgcccatc tcggtcgagc
     3601 cctggatcag cgtgaccttg ccctcgccca cgttgcccgc cgggtccgtg ccggtgatcg
     3661 tgatgacgtt cgtgccgggc tcgaggagca tcccgaacgc gaacttgccg tccttcgccg
     3721 acatcgacga gatcgacgtg tcgttcgcgg cgttcagcgc cacgagcgtc gtgttcgcct
     3781 gggtcgtgcc ctcgatggtg atctcgcggg cgtcgatcgc ggcctcgttc ttcggcttcg
     3841 tcacgctgat cttcggcggg tcggtgtcga ggatccaggt gacgatcggc gattgctccg
     3901 actcctcgtc accgcggaac agcgtcgccg atatgttgtt cttgcccttg gtgagctcga
     3961 acggaaccga catccggctc gtctcgctga cgggcacgtc gacgatcggc gccggctcga
     4021 ggccctcgag ggcgagatag atgcggacct tcgccgtcgg gtcgccgacg acctcgatcg
     4081 gcaccgacac gctgagcgtg atcagcgcga cgttcgtgaa cggctgcgcg ggcgccgcga
     4141 tccgcggcga gtcggtcggc gggagctggg tcgagctggg caggggcgtt gcggtgaggc
     4201 ggtcgaaggc cgtcgagaag ccggcgccga gggtggtgag gatcggcccg aggccgccgc
     4261 tcgcggtcat gaaggcaacg ccgccgatca ccgcgacggc gacggcgagc aaggtccgcg
     4321 tcgcgagcgg cgcgcgcttg cgccggccct cgaggccgcg atgctggcgt acgcggcggc
     4381 gatcgggtgc ggcgaccttg accgcttggg tcgtgcggcc gctcgagggc ggccgagggc
     4441 gaacgtggga gctggctcgg cgggaaggca tctgttccta tcgtcgcatg cgctggggca
     4501 cgtccgccgc attgcagtct ggtcttgtgc ggttcggggc actgctccga ggtcgttgcc
     4561 cggtgtacgc tgaatcggcg cggccatcgg cgccgctgga gtgggggagt ccgtgcaacc
     4621 tggcggtccc agaacgggcc ggggcgtccg agtgaccggt cgcccgccgt tcggcatcgg
     4681 cccgatctcc cgggtgtcga ccgtgcccac gccgggcgcc gattcggccc cggaccgttt
     4741 cgtcgcgggc ggggcgacgc gccccgcgga gcagcgctac gggatcaaca tcggcggcgg
     4801 ccccgcccgg ttcggtgccg accggacccg cgccttcggc ctgcccaaca ttcgccgtaa
     4861 cgtctgcccg cacttctacg tgaccgggtc gcgcggcgcg ctgccgatcc atgcgccgca
     4921 cctgctcaag ggcatcgtcc gccaggacag cgtcgtctcg cgctgccagt tgcgcggtgg
     4981 cgtccacctc gagcagagct acgtcaacga cgtctgcctc gggccgcggt tcaaccagtg
     5041 cgtgttctac gaggaggagc tgagcgcgcg ttgagccgct cgggctcccc tgccgcggac
     5101 gcgtccgcgt accacgcggc cgtccaggcg ctggccgagc gcggtcgctt cggggtccgg
     5161 ctggggctgg ctcgaacacg cgccctcctg cgagctctcg gcgacccaca gctcgagctg
     5221 cggggcgccc tgatcggcgg gaccaacggc aagggcagcg tcctcgccct ggcctcgagc
     5281 gccctgcgcg ccgccggcat ccggaccggc gccacgccca agccgcacct cgtgagctat
     5341 cgggagcggc tggagatcga cggccggccg atcgacccgg tgacgttcac ccggctcgt
//
LOCUS       DATBSW010000123         6074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_538932, whole genome shotgun sequence.
ACCESSION   DATBSW010000123 DATBSW010000000
VERSION     DATBSW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538932"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VIF63_03420"
     CDS             <1..887
                     /locus_tag="VIF63_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8238463.1"
                     /translation="TGSGAAPVVAQIAKELGALTIAVVTKPFTFEGAKRRLTAEKAGE
                     SLKANVDTLITIPNDRLRDVVQKNTSILDAFRVVDDVLRQGVQGISDIITVPGLINLD
                     FADVRAIMKDAGSALMGIGKASGENRAVEAARQAIASPLLEVDIAGAQGILFNISGGP
                     SMSLFEVTEAAEEIRAAADPEANIIFGTSFNERLGEEVAITVIATGFDGKRHGSSSRA
                     ATSVNADGSARAPRDFLEELERQRSSMNETGVPDAPAARPDEGAAAATRVVEPVAARR
                     PAYDADDLEIPSFLRRTR"
     gene            1144..1872
                     /locus_tag="VIF63_03425"
     CDS             1144..1872
                     /locus_tag="VIF63_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY8238464.1"
                     /translation="MTDLDAFGTVRDAVLRQIATAADRVGRDPAAVQLVAVTKTVDAE
                     RIRAAIAVGFTTLGENRVQERAGKVAEVDAVSTYPAGEAPRWHLVGPLQSNKARRAIE
                     LFDVIETVDSLDLAERLSRIAGEVRPGNPLPVLLQVNVDDDSAKSGFDPAALEAALPQ
                     ILDLPGLVVDGLMTVGRLVDTPEKARPTFVALRRLSERLRASNPALGGQLSMGMSEDY
                     PVAVEEGATLVRIGRAIFGARQPR"
     gene            1893..2147
                     /locus_tag="VIF63_03430"
     CDS             1893..2147
                     /locus_tag="VIF63_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY8238465.1"
                     /translation="MRLTPRAAVNRIDGVVDGTLAIRVTAAPVDGAANTALIRLVAAA
                     VGVPRTRVALVVGASSRRKLIEIEGLEAATVRARWPGLDV"
     gene            2186..2261
                     /locus_tag="VIF63_03435"
     tRNA            2186..2261
                     /locus_tag="VIF63_03435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2219..2221,aa:Val,seq:cac)
     gene            2873..4168
                     /locus_tag="VIF63_03440"
     CDS             2873..4168
                     /locus_tag="VIF63_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238466.1"
                     /translation="MTDTAPAPVPERRGLWRFLPGLLAEPEFRRFWIGQTISSFGDQI
                     TWLALPVIAVLILHADPAQMGLLTAFGLLPHLLFSLPAGVWLDRVEQRRRLMILMDLG
                     RAMALAAIPVGVYLNWLSIPLLFLISFVVGTMSLVFTICWGSLFVAVAKREQYVEANS
                     LLNGSRSVSSVGGPAIAGVLIQVLSAPITLLFDALSFVGSALFLSRIRARDLPIEHEP
                     GSIRSQLAEGLRFTFRDPIVRPSVLAASTMNLFNYGFQGLFVLYVLTYLGVEPGILGL
                     ALGAGAVGSVVGALLAGRVGRRLGVGGAFLAGVILFPGATILIPIASPDMPMPLIVAF
                     LFAAEFVSGFGVMILDINAGAMLFARTPDRIRGRAMGAFGFINNGVRPIGAVVGGLIG
                     AAFGVRETLFVTTILSLAGALWLVGTPIIGLRDLPPATE"
     gene            complement(4200..>6074)
                     /gene="acnA"
                     /locus_tag="VIF63_03445"
     CDS             complement(4200..>6074)
                     /gene="acnA"
                     /locus_tag="VIF63_03445"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY8238467.1"
                     /translation="YLRLTGRSAERVALVEAYARAQGLWRLAGADPVFDDLLTLDLGS
                     VEPSVAGPRRPQDRVPLTGLRDNFRSAFPDGLRAMSQAKLEGLVPEGGQVEESSAESF
                     PASDPPSYATAAATIDPPAAAPDAGGDPADADYPSVTVEVNGERASIRTGSVAIAAIT
                     SCTNTSNPTVMVGAALLARNAVARGLRVSPTVKTSLAPGSKAVTTYLEKAGLMAPLET
                     LGFALAGYGCTTCIGNSGPLDEPIAKAIEDNELVTAAVLSGNRNFEGRIHPLARASYL
                     ASPPLVVAFALAGRVDIDLTTEPLGIDDDGKRVFLADLWPSSEEIRSVIHDSIDPELF
                     TTTYASVFEGDEKWRALPIPDGDRYDWDPRSTYVARPPYFDGMSMALPEITDIVGARA
                     LAILGDSVTTDHISPAGSIAPWSPAGEWLQERNISPLEFNSYGARRGHHDVLIRGTFG
                     NIRLHNALAGDKEGPYGVHLPDGEPGFIYDIAVRYRKEGVPLIVVAGKEYGSGSSRDW
                     AAKGPLLLGVRAVIAQSFERIHRTNLVGMGVLPLEFLPGESLASLGLTGRESFTIAGV
                     ADGLAPRSQLRVVARTDDGTTSSFAVTCRIDGPIELDYYRNGGILPAVLRRLAREGNV
                     "
BASE COUNT          863 a         2114 c         2151 g          946 t
ORIGIN      
        1 gcaccggctc gggcgcggcc ccggtggtgg cccagatcgc caaggagctc ggcgcgctga
       61 cgatcgcggt cgtcaccaag ccgttcacct tcgagggcgc aaagcgtcgc ctgacggccg
      121 agaaggctgg cgagtcgctc aaggccaacg tggacacgct gatcaccatc ccgaacgacc
      181 ggcttcgcga cgtcgtccag aagaacacgt cgatcctgga cgcgttccgg gtcgtcgacg
      241 atgtcctgcg ccagggcgtc cagggcatca gcgacatcat caccgtgccc ggcctcatca
      301 acctcgactt cgccgacgtg cgggcgatca tgaaggacgc cggctcggcg ctcatgggca
      361 tcggcaaggc gtcgggcgag aaccgggccg tcgaggcggc gcggcaggcg atcgccagcc
      421 cgctgctcga ggtggacatc gcgggcgcgc agggcatcct gttcaacatc tccggcggcc
      481 cgagcatgtc gctcttcgag gtcaccgagg cggcggagga gatccgtgcc gcggccgatc
      541 ccgaggcgaa catcatcttc ggcaccagct tcaacgagcg cctcggcgaa gaggtcgcga
      601 tcaccgtgat cgccaccggc ttcgacggca agcgccacgg ctcatcgtca cgcgcggcca
      661 cgagcgtcaa cgccgacggc agcgcgcgag ccccgcggga cttcctcgag gagctcgagc
      721 gccagcgcag ctcgatgaac gagaccggcg tccccgacgc cccggccgca cggcccgacg
      781 aaggcgccgc cgcagcaacg cgcgtcgtcg agccggtcgc tgcgcgtcgc ccggcctacg
      841 acgccgacga cttggaaatc ccgagtttct tgcgccgcac ccgctaatcc gctcggggcc
      901 atcagcgcga gtcccaggac acggagtact cgacccggtt acggtcggat tctccccggg
      961 gtagcgttcg gtaggactcg gcacggccca acctgacgct gcgagcggat ttgtcccgcg
     1021 ccacgctcac gtcgggatga aatggtcggc cgtggccggg ccgaacggag tcgaccggtg
     1081 tcgagtgcta cccacggtgt catccgacag gccgtggcgc gctcggggct atagcatcgc
     1141 ctcgtgactg atctcgacgc gttcgggacc gtgcgggatg ccgtcctgcg ccagatcgcc
     1201 acagccgccg atcgggtcgg acgcgatccg gccgcagtgc agcttgtcgc ggtcacgaag
     1261 accgttgatg ccgagcgcat ccgcgctgcg atcgcggtcg ggttcacgac gctcggcgag
     1321 aaccgggttc aggagcgcgc aggcaaggtc gccgaggtcg atgcagtgtc gacgtacccc
     1381 gcaggggagg cgccgcgttg gcatctcgtc gggccgctcc agtcgaacaa ggcgcggcgc
     1441 gcgatcgagc tgttcgatgt catcgagacc gtcgactcac ttgacctcgc cgagcggttg
     1501 agccggatcg cgggcgaggt gcggccgggc aacccgctcc cggtcttgct ccaggtgaat
     1561 gtcgacgacg acagcgcgaa gtcgggcttc gatcccgctg ccctcgaggc ggccctgccc
     1621 cagatcctcg acctgcccgg gctggtcgtc gacggcctca tgaccgttgg ccggctggtc
     1681 gacacgcccg agaaggcgcg cccgacgttc gtcgcgctgc gccggctgtc ggagcggctc
     1741 cgagcgagca atccggccct cggcggccag ctctcgatgg gcatgagcga ggactatccc
     1801 gtcgcggtcg aggagggcgc cacgctcgtc cgcatcggcc gcgccatctt cggcgcgcgg
     1861 caaccgcgat aggcacgagc gcccgcatcc cgattcgcct gacaccgcgc gccgcggtca
     1921 atcggatcga cggcgtagtc gacggcacgc tcgcgattcg ggtcacggcc gcgccggtcg
     1981 atggcgccgc caacaccgcg ctgatcaggc tcgtcgctgc cgccgtgggg gtgcccagga
     2041 cgcgcgtcgc actcgtcgtc ggggcgtcga gccggcgcaa gctgatcgag atcgaggggc
     2101 tcgaggccgc caccgtccgg gctcgctggc cgggcctcga tgtatgatcc gcacccgccg
     2161 cggctcctcg agccggcgga ctcgggggcg attggctcag tggttagagc acgcggttca
     2221 catccgcggg gtcgctggtt cgaatccagc atcgcccacc atccctcacc ggctgatgcg
     2281 gcccgatcgc tgcgttgccg ccgtcgagtc gtcagtcacg tgcatgcgag cacgctccct
     2341 ccgcctctcc gtcggcgcct tgcgctcggg accgcctgag ccggtgaggc ctcgccggct
     2401 gatccgcggc catcgcggag ccggtgatcg cctcgtcggc gagcaaggca tcgcgtccca
     2461 gcgacccctt ggcgacctag tggatggtga tgttcaccag atgatcagat tcggggtttc
     2521 gaaggccctt tgggtcgcga cagccggaag cttcgatggt cggcgtggtc gatttgtccc
     2581 gtgattcgcc gatctgagca cgaatctggc cgcgccgagc gcaagtcgcc cgacaactag
     2641 gcccgggcag ggccccgttc ggccgcatcg aggccgaaat gccctgccaa acgccagcat
     2701 tgatcatgac gtgaacatcg agggccctta gggtccatcg cgtgggtcat ggcagtcgga
     2761 gccgcaccac gtggaggcct tcggagcgcg ctaggatcgg gtggcattcc tggcctcgga
     2821 gtgcgccggc cgaaacccgg cccagcgcat gcacctggag gccatcgacg cgatgactga
     2881 tacggcgcca gcgcccgtcc ccgagcgccg cgggctgtgg cgattcctgc cgggcctgct
     2941 ggcggagccg gagttccggc ggttctggat cggccagacc atctcctcgt tcggggatca
     3001 gatcacgtgg cttgcattgc ccgtgatcgc ggtcctgatc ctgcacgccg atccggcgca
     3061 gatgggcctg ctcaccgcgt tcgggctgct gccgcacctg ctgttctcac tgccggccgg
     3121 ggtctggctc gaccgcgtcg agcagcgccg gcgcctgatg atcctgatgg acctcggccg
     3181 ggccatggcc cttgccgcga tcccggtcgg cgtctatctg aattggctca gcatcccgct
     3241 gctgttcctc atctcgttcg tcgtggggac gatgtcgctg gtcttcacga tctgctgggg
     3301 ctcgctcttc gtcgcggtcg ccaagcgcga gcagtacgtc gaggcgaact cgctcctcaa
     3361 cggcagtcga tcggtctcga gcgtcggcgg cccggcgatc gcgggcgtcc tcatccaggt
     3421 gctcagcgca ccgatcaccc tgctcttcga cgccctctcg ttcgtcggct cggcgctctt
     3481 cctcagtcgg atccgggcca gggacctgcc gatcgagcac gagcccgggt cgatccggtc
     3541 gcagctcgcc gagggcctgc ggttcacctt ccgcgacccg atcgtccggc cgagcgtgct
     3601 cgccgcctcg acgatgaacc tcttcaacta cggcttccag ggcctgttcg tgctctacgt
     3661 cctgacgtac ctcggggttg agccggggat cctcggcctc gccctcggcg cgggcgcggt
     3721 cggctcggtt gtcggcgcat tgctggcggg ccgggtcgga cggcggctcg gtgtcggtgg
     3781 cgcgttcctc gcgggcgtga tcctgtttcc cggcgcgacg atcctgatcc cgatcgcgtc
     3841 accagacatg ccgatgccgc tcatcgtcgc gttcctgttc gcggccgagt tcgtgtcggg
     3901 cttcggcgtg atgatcctcg acatcaacgc cggggcgatg ctcttcgccc ggaccccgga
     3961 tcgcatccgc ggtcgagcca tgggcgcgtt cgggttcatc aacaacggcg tgcggccgat
     4021 cggcgcggtc gtcggcggcc tcatcggcgc ggcgttcggt gtccgcgaga cactgttcgt
     4081 gaccacgatc ctgtcgctgg ccggcgcgct gtggctcgtg ggcacaccga tcatcgggct
     4141 gcgcgacctg ccaccggcga ccgagtgacg ggagcgacgg gaagggcgag agcggcgggc
     4201 tagacgttgc cctcgcgcgc gagccgtcga agcacggccg gcaggatgcc gccgttgcgg
     4261 tagtagtcga gctcgatcgg gccgtcgatc cggcacgtga cggcaaagga gctcgtcgtg
     4321 ccgtcgtcgg tccgagcgac gacccgcagt tgcgaccgcg gcgccagccc atcggcgacg
     4381 ccggcgatcg tgaacgactc gcgaccggtc agccccaggg acgcgaggct ctcgccgggc
     4441 aggaactcga ggggcaggac gcccatcccg accaggttcg tccggtggat gcgctcgaac
     4501 gactgcgcga tcacggcgcg cacgccgagc agcagggggc ccttggccgc ccagtcgcgc
     4561 gatgagcccg agccgtactc cttgccggcg acgacgatca gcggcacgcc ttccttgcgg
     4621 tagcgcaccg cgatgtcgta gatgaacccg ggctcgccgt ccggcaggtg cacgccgtac
     4681 gggccctcct tgtcgccggc gagcgcgttg tgcagccgga tgttgccgaa cgtgccgcgg
     4741 ataaggacat cgtggtggcc gcggcgcgcg ccgtacgagt tgaactcgag cgggctgatg
     4801 ttgcgctcct ggagccactc gccggcgggc gaccacggcg cgatcgagcc ggcgggggag
     4861 atgtggtcgg tggtgaccga atcgccgagg atcgcgagcg cgcgggcgcc aacgatgtcg
     4921 gtgatctcgg gcagggccat cgacatcccg tcgaagtacg gcggcctggc gacataggtc
     4981 gagcgcgggt cccagtcgta gcggtcgccg tccgggatcg gcagcgcgcg ccacttctcg
     5041 tcgccctcga agacgctcgc gtaggtggtc gtgaacagct cggggtcgat cgagtcgtgg
     5101 atgacgctgc ggatctcctc cgacgacggc cacaggtcgg cgaggaagac gcgcttgccg
     5161 tcgtcatcga tgccgagcgg ctccgtcgtg agatcgatgt cgacccgacc ggccagcgca
     5221 aacgcgacga cgaggggcgg cgaggcgaga tagctcgcgc gcgcgagcgg atggatgcgg
     5281 ccctcgaagt tccgattgcc ggacaggacc gccgccgtca ccagctcgtt gtcctcgatc
     5341 gccttcgcga tcggctcgtc gagcggcccg gagttgccga tgcaggtggt gcagccgtaa
     5401 ccggcgagcg cgaagccgag cgtctcgagc ggcgccatga gccccgcctt ctcgaggtag
     5461 gtggtgaccg ccttcgagcc gggcgcaagc gaggtcttga ccgtagggga cacccgcagg
     5521 ccccgggcga ccgcattccg agcgagcagc gccgcgccca ccatcacggt cgggttggag
     5581 gtgttggtgc acgaggtgat ggccgcgatc gcgaccgagc cggtcctgat cgaggcgcgc
     5641 tcgccgttga cctcgacggt cacggacggg taatcggcgt cggccgggtc gccgcccgcg
     5701 tccggcgccg ccgccggtgg gtcgatggtc gcagccgccg tggcatacga gggcgggtcg
     5761 ctcgccggga atgactcggc cgacgattcc tccacctggc cgccctcggg caccaggccc
     5821 tcgagcttcg cctgggacat cgcccgcagc ccatcgggga acgccgagcg gaagttgtcg
     5881 cgcagccccg tcagtggcac gcgatcctgg ggccgccgcg gcccggcgac cgagggctcg
     5941 accgagccca ggtcgagcgt gagcaggtcg tcgaagaccg ggtccgcgcc cgccagccgc
     6001 cacaggccct gggcccgggc gtatgcctcg acgagcgcca cgcgctcggc ggagcggccg
     6061 gtgaggcgca ggta
//
LOCUS       DATBSW010000124         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1138059, whole genome shotgun sequence.
ACCESSION   DATBSW010000124 DATBSW010000000
VERSION     DATBSW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138059"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VIF63_03450"
     CDS             <1..288
                     /locus_tag="VIF63_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY8238468.1"
                     /translation="SATPSTVYRTLDVLEDIGVVSHSHGREGRQEFHVNPAADHGHLL
                     CSSCGGTWELTPDEVQNLVAGLRAGRGFDVAVDHLTIEGRCAACREAAGVA"
     gene            344..2407
                     /locus_tag="VIF63_03455"
     CDS             344..2407
                     /locus_tag="VIF63_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8238469.1"
                     /translation="MPPLNPNPRQLKGRKLGDRRVKIERPHAAYFRYTPEGTLVAKEA
                     ASLPRTQVGRAYARFRGILFGRPLSIHEELTQRLSKVKALAIFSSDPISSSAYATEEI
                     LRVLVVAGSGALFLSLPVAAAIALLLGVVSISYRQIVHAYPSGGGDYAVARRNLPALG
                     SLVVAGALLVDYVMTVAVSTASAAEQVVSAVPEVAEWKLVIGVTAIILITIGNLRGLR
                     ESGSIFAIPTYLFVGSALVMIAIGLFRVVVNGEGSPQVIPPSSGTPDSLQAVGILLMI
                     RAFASGSVALTGTEAIANGVPAFKPPESKNAATTLTAVAILLAILFLGITFVADSFGI
                     LPTPDGQVPKTVISQVAAVIYGADTIPFYLFQTFTALILFLAANTSYNAFPRLAAILA
                     TDGYMPRQFSFRGDRLAFTSGVVILSVVAITLLLAFGGQTHALIPLYSVGVFIAFTVG
                     QSGMIVHWLRERPPGWQWRLAINSFGCALTGLVAVVVMVAKAPTSLLVIVVIPLLVGM
                     MMFINRQYRASKAELAVRPNAVIRGPHREERVIVPVPGINRAVVQAVNVGLSIDPNVQ
                     AVLISDEPEEAVEIRKRWEKQLPDVPLVIVESPYRALIAPLLSYLDVLDRAWPSDKEA
                     PITFVVIPEYVARSWWERILYNQSAKRLRTALLGRAHTVVVNVPYRREEGDEPDRPAA
                     PPPPA"
     gene            2412..>2843
                     /locus_tag="VIF63_03460"
     CDS             2412..>2843
                     /locus_tag="VIF63_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238470.1"
                     /translation="MSPGDRSSTDGAAWRLVPLLVAAALVRIIGLFGPGHLGDITAFV
                     QWAEDAARLGLGNYYLGGGNANYPPMLYLLWPLGVALDRFDLVLAIRALSIPFDLALG
                     VLLFRTVRSATGRNDDGRLAAAFYLLNPAVVLVGPAWGQVDG"
BASE COUNT          383 a         1024 c          941 g          495 t
ORIGIN      
        1 tccgccacgc cgtcgacggt gtaccgtacg ctcgacgtcc ttgaggacat cggcgtcgtc
       61 agccacagcc acggccgcga gggccggcag gagttccacg tcaaccctgc ggccgatcac
      121 ggccatctgc tgtgctcgag ctgtggcggg acgtgggagc tgacgcccga cgaggttcag
      181 aacctcgtcg ccggcctgcg cgcgggccgc gggttcgatg tcgccgtcga ccacctgacg
      241 atcgaagggc gctgcgccgc ctgtcgcgag gccgccgggg tcgcctgact cggacgatcg
      301 ccctcggcgc atgcgatcct ctgcggatgc acgcaggacg gcgatgccgc cgctgaaccc
      361 caacccccga cagctcaagg gtcgcaagct cggcgaccgg cgggtcaaga tcgagcggcc
      421 gcacgccgcc tatttccggt acacgcccga gggcacgctc gtggcaaagg aggcggcgtc
      481 gttgccgcgc acgcaggtcg ggcgtgccta cgcccgcttc cgcggcatcc tcttcgggcg
      541 tccgctctcg atccacgagg agctgaccca gcgcctgtcc aaggtcaagg cgctcgcgat
      601 cttcagctcg gacccgatca gctcgtccgc ctacgcgacg gaagagatcc tgcgcgtcct
      661 ggtggtcgcg ggctccggcg cgctgttcct gagcctgccg gtcgcggcgg caatcgccct
      721 cctcctcggc gttgtctcga tcagctaccg ccagatcgtg catgcctatc cgtccggtgg
      781 cggcgactac gcggtcgcgc gccgcaacct gccggcgctc ggctcgctcg tcgtcgccgg
      841 tgcgctgctc gtcgactacg tcatgaccgt cgcggtctcg accgcctcgg cggcggagca
      901 ggtcgtctcg gcagtgcccg aggtggccga gtggaagctc gtcatcggcg tcaccgccat
      961 catcctgatc acgatcggca acctacgcgg gctgcgcgag tcgggcagca tctttgccat
     1021 cccgacctac ctgttcgtgg gctcggcgct tgtgatgatc gccatcgggc tcttccgggt
     1081 cgtagtcaac ggcgagggca gcccgcaggt gatcccgccg tcgagcggca cgcccgattc
     1141 gctgcaggcc gtgggcatcc tgctgatgat tcgggcgttc gcgtcaggct ccgtcgcgct
     1201 gaccggcacc gaggcgatcg ccaacggcgt gcccgcgttc aagccgcccg agtcgaagaa
     1261 cgccgcgacc acgctcaccg ctgtcgcgat cctgctggcc atcctgttcc tcggcatcac
     1321 gttcgtcgcg gactcgttcg gaatcctgcc cacgcccgac ggccaggtgc cgaagacagt
     1381 catcagccag gtcgccgccg tgatctatgg cgccgacacg atcccgttct acctgttcca
     1441 gacgttcacc gccctgatcc tcttcctcgc ggcaaacacg tcgtacaacg cgttcccacg
     1501 cctcgccgcg atcctcgcca ccgacggcta catgccccgc cagttcagct tccgtggcga
     1561 tcggctcgcg ttcacctcgg gcgtcgtgat cctgagcgtc gtggcgatca cgctcctgct
     1621 ggcgttcggc ggccagaccc acgccctgat cccgctgtac tcggtcggcg tgttcatcgc
     1681 gttcaccgtc gggcagtcgg ggatgatcgt ccactggctg cgcgagcggc cgcccggctg
     1741 gcagtggcgc ctcgcgatca actcgttcgg ctgcgcgctg acagggctcg tcgccgtcgt
     1801 ggtcatggtc gccaaagccc cgacgtcgct gctcgtcatc gtcgtgatcc cgctgctcgt
     1861 cgggatgatg atgttcatca atcggcagta ccgcgcgtcg aaggcggagc tcgccgtcag
     1921 gcccaacgcg gtcatccggg gcccgcatcg cgaggagcgc gtgatcgtgc ccgtacccgg
     1981 gatcaaccgg gccgtcgtcc aggcggtgaa cgtcgggctg tcgatcgatc cgaacgtcca
     2041 ggcggtgctc atctccgacg agcccgagga ggcggtcgag atccgcaagc gctgggagaa
     2101 gcagctgccc gacgttccgc tcgtcatcgt cgagtcgccg tatcgcgccc tcatcgcgcc
     2161 gctcctctcg tacctcgacg tcctcgatcg tgcctggcct tcggacaagg aggcgccgat
     2221 cacgttcgtg gtcatcccgg agtacgtggc gcgcagctgg tgggaacgga tcctctacaa
     2281 ccagtcggcg aagcggctgc gaacggcgct gctgggccgg gcgcacacgg tcgtcgtcaa
     2341 cgtgccgtac cggcgcgagg agggtgatga gcccgatcga ccggcggccc cgccgcctcc
     2401 cgcatgatcg ggtgagcccg ggggatcgat cgagcacgga cggtgcggcg tggcgtctcg
     2461 tgcccctgct cgtggctgcg gcgctggtgc gcatcatcgg gctgttcggg ccaggccacc
     2521 tcggcgacat caccgcattt gtccagtggg ccgaggacgc ggcccggttg ggcctgggca
     2581 actactacct cggcggcggc aacgccaact acccgccgat gctgtatttg ctgtggccgc
     2641 tcggcgtcgc ccttgacagg ttcgacctcg tgctggccat ccgggcgctg tcgatcccgt
     2701 tcgacctcgc gttgggcgtg ctcctgttcc gaacggtccg ctccgcgacc ggcaggaacg
     2761 acgacggacg tctcgccgcc gcgttctacc tgctgaaccc agcggtcgtc ctcgtcggac
     2821 cggcgtgggg ccaggtcgat ggc
//
LOCUS       DATBSW010000125         7455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1244258, whole genome shotgun sequence.
ACCESSION   DATBSW010000125 DATBSW010000000
VERSION     DATBSW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244258"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VIF63_03465"
     CDS             complement(<1..1700)
                     /locus_tag="VIF63_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238471.1"
                     /translation="MRAARSAMPARFMGAFALAMVTAFGLVPGLAPTEVEAATPKLTI
                     VGAATYDVLPEEGRVAVTVQLTAKNHLKNTATKQFFFRSGFVTVLPGTSNFRLTGGSA
                     TPKVTVSSKTETYTNLKIDFGANLGAGKSTKLTLTFDVLDPGGAPDRALRISHSLVAF
                     VAWAVATPETPGASVDVRLPVGYGVTVRRGPLDGPTAADATHEVWSSGTLAKPLDYVA
                     EIAADRPTEFAESSLDVAMSGGPATVVVRSWPDDPNWRDRVSSLVERALPLLERDIGL
                     DWPVDGPLAVHEALVRNTGGYAGIFDPAEGRIDIAYAASDGVVLHELAHAWFNGRLVA
                     DRWAAEAFASYYAGLAAVELGIDPAAPTLPDAPSPAAIPLNAWRPSGIEDPETEAWAY
                     AASLQLAKLIAERAGPAAQHAVWADAALGIGAYQPESSSGALGGAEPAAGPPDWRGLL
                     DLFEAKADVDLGDLWRTWVARPEDGQMLADRAIARGFYARSVALAGEWQLPPAIRQAM
                     RAWRFDVAQELILAADAVQVQRRRLETAAAAAEATLPDRLRTAFEGTGGVAAAAAEAS
                     A"
     gene            complement(1697..2209)
                     /locus_tag="VIF63_03470"
     CDS             complement(1697..2209)
                     /locus_tag="VIF63_03470"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY8238472.1"
                     /translation="MPPTTPDAVERWLAELGLEPGARAERDGVVAWDLTLDGRKRRDL
                     RVTLILDSSLGAIVWAHLAPPLLDGLRKSYRTLLRWNDEFPLVKFSIADDGRPTAAVE
                     IPTRWLDADELGLALVRVTSVADRLFDETRGWLWIGGRVPDNYADRPIRNAVLLDRFE
                     PALGELVEAG"
     gene            complement(2269..3591)
                     /locus_tag="VIF63_03475"
     CDS             complement(2269..3591)
                     /locus_tag="VIF63_03475"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY8238473.1"
                     /translation="MKRTMALILAGGEGERLSILSSVRAKPAVPFGGKYRIIDFSLSN
                     CVNSDITDILVLTQYNPRSLNDHIGVGRPWDLDRNRGGVKLLQPYIARGKVAEWYRGT
                     ADAVLQNLNVIERSDADTIVVLAGDHIYKMDYQPFVAAHRRHRADVTIAVRRVPLADA
                     SRMGILAMDDNDRVTDWQEKPKQPKSDLASMGVYVFTKRALRRWLSEDRFDFGGNVIP
                     AMLDGGARVYGYRFSGYWQDVGTIQSFWEANLALCEDRPELDLYDQDWVIHTRSEERA
                     PAKVGATAQIHKSLISHGCVIDGTVVNSVLSPGVRVDVGAVVRDSIVMFDSVIRAGAV
                     VDRAILDKEVVVGQGAIVGEGPYDGPPNKQEPGRLNTGITVVGKQSIVPRGARIGRNV
                     KIGGEVKSADYATRVIRAGGSVETGVAGRRTRATKAAAIARDEAASAG"
     gene            complement(3638..5002)
                     /locus_tag="VIF63_03480"
     CDS             complement(3638..5002)
                     /locus_tag="VIF63_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8238474.1"
                     /translation="MRRSTPDRRGWSASRRARFGRRAVLFLFVLSTFGGLFTSTPPKT
                     AQADELSDAYKKQQALQKLIDKQRASIKALTANQGILSGRISKTKHSLTEINANLLTA
                     KTQVVAMVVDVARSQNAVDELDATSARLDAELAQIEADEATKQADLDDRVELLSSRIR
                     EAYNTDRTSILDTFLSGNDFTDVLTEAAYHLEFAEQDKLLAEQIVEDKKVLEVLHQNV
                     VLARQQTEELHQLAATSKAALDAQLTDLKEARAELAKLEAETARLLKAQQAAYAKMAA
                     NKTRLAASLKAQLEADRKLEALINRLVKAALAKGGIPSQYNGTFRWPLDGRITQEFGC
                     TGFSWEPAYGNCRHFHNGIDIANSMYTPVYAAGPGKVIWSGRSPYSSSWNVIIAHSSH
                     LVTWYAHLDNKGHKPVVRAGQYVDKGQLIAYEGMTGNTTGPHLHWAVQLNENWVNPRL
                     FLPR"
     gene            complement(5168..5821)
                     /locus_tag="VIF63_03485"
     CDS             complement(5168..5821)
                     /locus_tag="VIF63_03485"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY8238475.1"
                     /translation="MDETLAIGVAGLLGLGWGYASDRIAARWPAHEDGSIRPIDWRTW
                     LVVAAGVLAFGATVARFGTAVDELLVMGVYVVALVLLFATDLDQRLLPNVITFPLIGL
                     ALVAFVTGSGPFVHTVEDLTWAAGAAVVVPLGLFLLAKPFGEGAIGEGDLKLLVSVGL
                     LAGAQNLVYGLIAGALLAGVVVAILVFVRRLTLKSFVPYGPFLIAGTLWAILALPRP"
     gene            complement(5865..>7455)
                     /locus_tag="VIF63_03490"
     CDS             complement(5865..>7455)
                     /locus_tag="VIF63_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8238476.1"
                     /translation="SRPDRDAARHYTRRRVCLTAIRRGSPSMQVRVVVDQPWLVKADV
                     LVVPIVGKPVFTGPLGQLDKQAGGELASLAKFGELTGKRFKSVLAASGGTAAGRLLAI
                     SAGDAADLDVEAVVKLAASAERRLAGRTVRSLAIWISPLASLEGGAAAVAGLVARGVV
                     EGSYDPASIYREGYDEGPPALDELILIAPDEDAAGLKKAAERGVIIGEGANIARTLSN
                     RSSNDVSPEVLAEEAYTLAKKHGLWIDVIGPEKATSMGMGLFMAVGRGSDNPPRMIVM
                     RSGKAGEKDALGRHLAIVGKGVCFDSGGISIKPSDRMEEMKMDKTGAATVIAAAITVQ
                     RLAPGTPLLTLAPTVENMPGPHSTRPGDVVKALNGKMVDITNTDAEGRLILADALTYA
                     EQLGATHLVDVATLTGAVSRALGELVSGAFGTPQSFVDDVLAAGAKAGERYWQLPLVE
                     EYRADMESWYGDLVNFSRNNEGGLVKSALFISEFVTKPWVHLDIAGTGYFRKVAPWAP
                     RGSTGVSHATLVELALAGAKA"
BASE COUNT         1083 a         2566 c         2679 g         1127 t
ORIGIN      
        1 gccgacgctt ccgcggcggc cgcggcgaca ccgcccgtgc cctcgaaggc ggtgcgcaac
       61 cggtcgggca gggtcgcctc ggccgcggcc gccgcggtct cgagccgacg ccgctgcacc
      121 tggacggcat ccgcggccag tatcagctcc tgcgccacgt cgaagcgcca cgcgcgcatg
      181 gcctggcgaa tcgcaggcgg gagctgccac tcccccgcca gtgccacgga tcgggcatag
      241 aagcctcgag caatcgcgcg atcggcgagc atctgcccgt cctccgggcg ggccacccag
      301 gtccgccaca ggtcaccgag atcgacgtcc gctttggcct cgaacaggtc gagcaagccg
      361 cgccagtccg gcggccccgc cgccggctcc gcgccgccca gcgcaccgct gctcgactcg
      421 ggctggtagg ccccgatccc gagcgcggcg tcggcccaca ccgcgtgctg cgccgccggc
      481 ccggcgcgct cggcgatgag cttcgcgagc tggagtgatg ccgcgtacgc ccacgcctcg
      541 gtttcgggat cctcgatccc gctcggtcgc caggcattga gcgggatggc cgccggcgag
      601 ggcgcatcgg gcagggtcgg cgccgcggga tcgatgccca gctccaccgc agcgaggccc
      661 gcgtagtacg aggcgaaggc ctccgcggcc cagcgatccg cgacgagccg gccgttgaac
      721 caggcgtggg ccagctcgtg gaggacgacg ccgtcggacg cggcgtaggc gatgtcgatc
      781 cggccctccg ccgggtcgaa gatgccggcg tagccaccgg tgttgcggac gagggcctcg
      841 tgcacggcga gcggcccgtc gaccggccag tcgaggccga tgtcgcgctc gaggagcggg
      901 agcgcccgct cgacgagcga gctcacgcga tcgcgccaat tcgggtcgtc gggccaggag
      961 cgcacgacga ccgttgccgg gcccccggac atcgcgacgt ccagcgacga ctccgcgaac
     1021 tcggtcggtc gatcggccgc gatctccgcg acgtagtcga gcggcttcgc gagcgtcccg
     1081 ctggaccaca cctcgtgggt cgcatcggca gcagtcgggc cgtcgagcgg accgcgccgg
     1141 accgtgacgc cgtagccgac gggcaggcga acgtcgaccg atgcaccggg ggtctcgggc
     1201 gtcgccaccg cccacgcgac gaaggccacg agcgaatggg agatgcgcaa cgcccgatca
     1261 ggcgcgccac ccgggtcgag cacgtcgaac gtcagggtga gcttcgtcga cttgcccgcg
     1321 ccgaggttcg ccccgaagtc gatcttgagg ttcgtgtagg tctcggtctt cgagctgacg
     1381 gtgaccttcg gcgtggcgct cccgcccgtg agccggaagt tggacgtgcc cggcagcacg
     1441 gtgacgaagc cgctccggaa gaagaactgc ttggtggcgg tgttcttgag gtggttcttc
     1501 gcggtcagct gcaccgtcac cgccacgcgg ccttcctcgg gcagcacgtc atacgtcgcc
     1561 gcgccgacga tcgtcagctt cggtgtcgcc gcctccacct cggtgggagc gagccccggt
     1621 acgagcccga acgccgtgac catcgccagg gcgaaggcgc ccatgaatcg cgccggcatg
     1681 gccgagcggg cggccctcat cctgcctcga ccagctcgcc gagcgccggc tcgaaccgat
     1741 cgagcagcac cgcattccgg atcgggcggt ccgcgtagtt gtccggcacc cgaccgccga
     1801 tccacaacca gccccgggtt tcgtcgaaca gccgatccgc gacgctcgtc acgcgcacga
     1861 gggccaggcc gagctcgtca gcgtcgagcc agcgcgtcgg gatctcgacg gcggccgtgg
     1921 gccgcccgtc gtcggcgatc gagaacttca cgagcgggaa ctcgtcgttc cagcggagca
     1981 gcgtccggta cgacttgcgg agcccgtcca ggagcggcgg cgccaggtgc gcccagacga
     2041 tcgcaccgag gctcgaatcg aggatcagcg tgacccgcag gtcgcgccgc ttgcggccat
     2101 cgagggtgag gtcccaggcc acaacgccgt cgcgctcagc gcgggcgccg ggctccaggc
     2161 cgagctcggc gagccagcgc tcgacggcat cgggggtggt cgggggcatg gcgcgccagt
     2221 ctagcccggg tcggccgagg cgccggtgcg cggccgttgc ggctgggcct agccggccga
     2281 ggcggcctcg tcgcgggcga tcgcggcggc cttcgtggcg cgggtgcggc ggcccgctac
     2341 cccggtttcg accgagccgc cggcgcggat gacgcgcgtt gcgtagtcgg cgctcttgac
     2401 ctcgccgccg atcttcacgt tgcgcccgat gcgcgcgccg cggggcacga tcgattgctt
     2461 gccgacgacg gtgatcccgg tgttcagccg gcccggctcc tgcttgttcg gtggcccgtc
     2521 gtacgggccc tcgccgacga tcgcgccctg gccgacgacg acctccttgt cgaggatcgc
     2581 ccgatcgacg accgcgccgg cccggatcac cgagtcgaac atcacgatcg aatcgcgcac
     2641 caccgccccg acatcgaccc ggacgcccgg cgagagaacg ctgttgacga cggtgccgtc
     2701 gatgacacag ccgtggctga tgaggctctt gtggatctgc gcagtcgccc cgaccttggc
     2761 cggcgcgcgc tcctcggacc gggtgtggat gacccagtcc tggtcgtaca ggtcgagctc
     2821 ggggcggtcc tcgcacaggg ccaggttcgc ctcccagaag gactggatcg tgccgacgtc
     2881 ctgccagtag ccggagaagc ggtagccgta gacgcgggcg ccaccgtcga gcatcgccgg
     2941 gatcacgttg ccgccgaagt cgaaccggtc ctcgctcagc cagcgccgca gcgcgcgctt
     3001 cgtgaacacg tacacgccca tcgacgcgag gtcgctcttg ggctgcttcg gcttctcctg
     3061 ccaatcggtg acgcgatcgt tgtcgtccat ggcgaggatg cccatgcgac tcgcgtcggc
     3121 gaggggcaca cggcggacgg cgatggtcac gtcggcgcga tgccggcggt gggcggccac
     3181 gaacggctgg tagtccatct tgtagatgtg gtcgccggcg agcacgacga tcgtgtccgc
     3241 gtcggagcgc tcgatgacgt tcaggttctg gagcaccgcg tcggccgtcc cgcggtacca
     3301 ctcggcgacc ttgccgcgcg cgatgtacgg ctggagcagc ttcacgccgc cgcggttccg
     3361 gtcgaggtcc cacggccggc ccacgccgat gtggtcgttg agcgagcgcg ggttgtactg
     3421 cgtcaggacg aggatgtcgg tgatgtccga gttgacgcag ttcgacagcg agaagtcgat
     3481 gatccggtac ttgcccccga agggcacggc gggcttggcc cgcaccgaag acaggatgga
     3541 caggcgctcg ccttcaccgc cggcgaggat cagcgccatc gtccgcttca caggcgaatc
     3601 gtagcaccgg gtccgtgccc ggccggttac cgctgcgtta gcgcggcagg aacaggcgcg
     3661 ggttcaccca gttctcgttg agctggaccg cccagtggag gtgcgggccg gtcgtgttgc
     3721 cggtcatgcc ctcgtaggcg atgagctggc ccttgtcgac gtactggccg gcgcgcacga
     3781 cgggcttgtg gcccttgttg tcgaggtgcg cgtaccaggt cacgagatgc gaggagtggg
     3841 cgatgatcac gttccaggac gacgagtacg ggctgcggcc ggaccagatg accttgcccg
     3901 ggccggctgc gtagaccggc gtgtacatcg agttggcgat gtcgatgccg ttgtggaagt
     3961 ggcggcagtt gccgtacgcg ggctcccacg agaagccggt gcagccgaac tcctgggtga
     4021 tccggccatc gagcggccac cggaacgtgc cgttgtactg ggacgggatg ccgcccttgg
     4081 cgagcgccgc cttgacgagc cggttgatga gcgcttccag ctttcgatcg gcctcgagct
     4141 gggccttgag cgacgctgcg agccgggtct tgttcgcggc catcttcgcg taggccgcct
     4201 gctgcgcctt gagcagccgc gcggtctcgg cctcgagctt ggccagctcg gcgcgggcct
     4261 ccttgaggtc cgtgagctgc gcgtcgagcg cggccttcga ggtggcggcg agctggtgga
     4321 gctcctcggt ctgctggcga gccagcacca cgttctggtg gaggacctcg aggaccttct
     4381 tgtcctcgac gatctgctcg gcgagcagct tgtcctgctc ggcgaactcg aggtggtaag
     4441 cggcctccgt gagcacgtcg gtgaagtcgt tgcccgagag gaacgtgtcg aggatcgagg
     4501 ttcgatcggt gttgtacgcc tcgcggatgc gcgaggacag gagctcgacc cggtcgtcga
     4561 ggtcggcctg cttggtggcc tcgtcggctt cgatctgggc gagctccgcg tcgagtcgcg
     4621 ccgacgtggc gtcgagctcg tcgaccgcgt tctggctgcg ggccacgtcg acgaccatcg
     4681 ccacgacctg cgtcttggcc gtcagcaggt tggcgttgat ctcggtgagc gagtgcttcg
     4741 tcttcgagat gcgcccggag aggatcccct ggttcgcggt cagcgccttg atcgaggcgc
     4801 gttgcttgtc gatgagcttc tggagcgcct gctgcttctt gtaggcgtcg gacagctcgt
     4861 cggcctgggc agtcttcggc ggcgtggacg tgaacagccc accgaacgtc gacagaacga
     4921 agaggaagag gacggcccgt cggccgaagc gcgcacgacg gctggctgac cagcctctgc
     4981 gatcaggcgt ggagcggcgc atcaagcagc gtcttcatcg tgcgggcgtc tccatcggcg
     5041 ctgggggccg cttaggtttc gctcgccggt gcagaacgcc gtcccgccta gagcacatgc
     5101 cgggacgact accggagaac gtcgagatac tactgcaacg tgtccagccc aaaaccccgt
     5161 acgttcgtca cggtcggggt agggcgagga tggcccacag cgtccccgcg atcaagaacg
     5221 gtccgtacgg tacgaagctt ttcagggtca ggcgccggac gaagaccagg attgccacca
     5281 cgacgcccgc gagaagggcg cccgcgatga ggccgtagac caggttctgc gcacccgcca
     5341 ggagcccgac gctgacgagg agcttgaggt caccctcgcc gatcgcgccc tcgccgaacg
     5401 gcttcgccag caggaacagg cccaggggca cgacgaccgc cgcaccggcg gcccaggtca
     5461 gatcctccac cgtgtggacg aaggggccgc tgccggtcac gaacgcgacg agggcgaggc
     5521 cgatgagcgg gaacgtgatc acgttcggca gcagccgctg gtcgaggtcc gtggcgaaga
     5581 gcaggacgag ggcgacgacg tacacgccca tcacgagcag ctcgtcgacc gctgtgccga
     5641 atctcgccac ggtggcgccg aaggcgagca cccccgcggc gacgacgagc caggtccgcc
     5701 aatcgatcgg gcggatcgag ccgtcctcgt gggccggcca gcgggcagcg atccgatccg
     5761 atgcgtagcc ccagcccagc ccgagcagcc ccgcgacccc gatggccagg gtctcgtcca
     5821 tcaggcgcga ccgcgcccgg cggacgccgt cgcgttgacc gtcgtcaggc cttcgcgccg
     5881 gcgagggcca gctcgacgag ggtcgcgtgg ctcacgcccg tcgagccgcg cggcgcccac
     5941 ggcgcgacct tgcggaaata gccggtgccg gcgatgtcga ggtggaccca gggcttggtg
     6001 acgaactcgc tgatgaacag cgcgctcttc accaggccgc cctcgttgtt gcgcgagaag
     6061 ttcacgaggt cgccgtacca gctctccatg tcggcgcggt actcctcgac gaggggcagc
     6121 tgccagtagc gctcgccggc cttcgcgccc gcggcgagca cgtcgtccac gaaggactgc
     6181 ggtgtcccga acgcgccgct gacaagctcg ccgagggcgc gtgagaccgc gccggtgagc
     6241 gtggccacgt cgacgaggtg ggtcgcgccg agctgctccg cgtaggtgag cgcgtcggcc
     6301 aggatcaacc ggccctcggc gtccgtgttg gtgatgtcga ccatcttgcc gttcagcgct
     6361 ttgacgacgt cgcccgggcg ggtcgagtgg gggccgggca tgttctcgac cgtcggggcg
     6421 agggtcagga gcggcgtgcc cggggcgagc cgctggaccg tgatcgcggc cgcgatgacc
     6481 gtcgcggcac cggtcttgtc catcttcatc tcttccatcc ggtcggatgg cttgatcgag
     6541 atgccgccgg agtcgaaaca cacgcccttg cccacgatcg cgagatggcg cccgagggca
     6601 tccttctcgc cggccttgcc ggagcgcatc acgatcatgc ggggcgggtt gtcgctgccc
     6661 cggccgacgg ccatgaacag gcccatgccc atcgaggtcg ccttctccgg cccgatgacg
     6721 tcgatccaca ggccgtgctt cttggcgagg gtgtacgcct cttcggcgag gacctcgggg
     6781 ctgacgtcgt tcgacgatcg gttcgagagc gtccgcgcga tgttcgcgcc ctcgccgatg
     6841 atcacgcccc gctccgcggc cttcttcagg cccgcggcgt cctcgtcggg cgcgatgagg
     6901 atgagctcgt cgagcgcggg cgggccttcg tcgtagccct cgcggtagat gctcgcaggg
     6961 tcgtagctgc cctcgacgac gccccgggcg acgagcccgg cgacagccgc ggcgccgcct
     7021 tcgagcgaag ccagcggcga gatccagatc gccagcgagc ggacggtgcg gcccgcgaga
     7081 cgacgttccg cggaggcggc gagcttgacc accgcctcga catcgaggtc ggcggcgtcg
     7141 ccggcactga tcgcgagcag gcgcccggca gccgtcccgc cggaggcggc aaggacgctc
     7201 ttgaaccgct tgccggtcag ctccccgaat ttggcgagtg aggcgagctc accgcccgcc
     7261 tgcttgtcga gctggcccag cgggccggtg aacaccggct tgccgacgat cggaacgacg
     7321 agcacatcag ccttgaccaa ccagggctgg tcgacgacga cgcgaacctg catggacggt
     7381 gatccccttc gaatggcggt caggcagacc cggcgccgag tgtagtgccg ggcggcgtcg
     7441 cggtcaggcc gcgac
//
LOCUS       DATBSW010000126        12243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_433448, whole genome shotgun sequence.
ACCESSION   DATBSW010000126 DATBSW010000000
VERSION     DATBSW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433448"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VIF63_03495"
     CDS             complement(<1..426)
                     /locus_tag="VIF63_03495"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY8238477.1"
                     /translation="MSPAAPAPRTVVVDGRSFERLPVRTPVLMPGNDMPGVIREHAGP
                     FLRAGDTVVMSESAVAIMQGRARDWRTIHPGRLARWMSARVIKTTWGTGLRSPYAMQY
                     AIELTGAPRMILAGVAHVIGRLLGKKGWFYLVAGLNARMM"
     gene            504..1574
                     /locus_tag="VIF63_03500"
     CDS             504..1574
                     /locus_tag="VIF63_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY8238478.1"
                     /translation="MSAPSPAAKLVRLASVSAALALLAALIVRPALGPVGARAMGELP
                     DCRYDDILTTPRGYGDWSITLVDTILRLPKSYAPPDLVRVVNLGVPGRGKVRAVIAED
                     LKAMSDAAAAANAAIAVSSAYRSYADQQAVFRSWVARYGYKRALQISARPGHSEHQLG
                     VTIDFRSQPDDGGELATSWAGTPAGKWMKAHAWEYGFLMSYPKGTMALTCYDWEPWHF
                     RYVGRELAAKIHASGLTPREYLWAHFTTTVVPPPPPPTHAPTKTARPSAAPTAAPSVA
                     PSPSPTALPTIAPETIAPPSASPAPTSSPATPAGSARPVVPQAPAAGFEPAVLAGGVV
                     VLGTTVLGLLFAIRRGRSGAGL"
     gene            1687..3045
                     /locus_tag="VIF63_03505"
     CDS             1687..3045
                     /locus_tag="VIF63_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEY8238479.1"
                     /translation="MHRTHRALLPFLVAAITVLVILPGFAQTTDAATTKWTAKCSINV
                     RTAPKTSATVKKVILKGAIVTASATVTGGSWKASCGGTTVSGSSWLKIVAINGKSTTS
                     LFGRSAVYAAKGLFKSGPNPTATPKPTAVPTAKPTAVVTAAPTAAPTAAPTAAPPTTT
                     NYISNCPVRLRATPSVNADTISIIDTNTVVTTATKVSGGAWAADCKTNVSGTSWYRIT
                     AIGGKSVSSLYGVSVVYAATGLFRSASTSGYREGIDVSHWQGAIDWAMVKGAGKTFAI
                     AKATEGINFKDDRYDRNKAAAMGQGIKFGAYHFARPENNAVTEADWFIDNAGYEHGMI
                     IPTLDLERTGGLGPTALTNWVKAWLQRVDQRLGVKPMIYMSPSFWRSNLNDSRWFADN
                     GYAILWVAHWGVTSPSIPGANWGGRSWTFWQYTSSGTVPGIAGRVDLNRYRFASFDAV
                     TY"
     gene            3093..3815
                     /locus_tag="VIF63_03510"
     CDS             3093..3815
                     /locus_tag="VIF63_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8238480.1"
                     /translation="MTTVSAGRPGQLLALVPAHDEAAGIGTVVKGARRHVPVLVVDDG
                     STDDTAALAEAAGARVIRQSPNQGKGAALRAGFAAALAEGAEAVITLDGDGQHDPSEI
                     PAFLGAYARQAIAGGQAELIVGRRDFRRMPLVRRLANGLGTLVLSGALGRWIPDNQSG
                     YRLIGRRLMQATLDSEEAGFAFEVEMIAVCQREGWPIGWVPVRTIYAGESSHIRPGKH
                     LREFLAVTRRARRIARSRPVSE"
     gene            complement(3833..6214)
                     /locus_tag="VIF63_03515"
     CDS             complement(3833..6214)
                     /locus_tag="VIF63_03515"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY8238481.1"
                     /translation="MTAGLLLAALAVGGLPILQSAVHRGVDIVRRDVEVAVDRQQLVH
                     LPIAASHVVLHWTGAPEAVLSIAVGRTPDQLSEEIPLGADEDADPAAVDSWSEVVWAG
                     GARYARVTSDRPIAHLTVSAIDADASRGIDQSGVVLAAVNQPAVITRAGWGANEDYSH
                     NAGGYVRFAPNYTPLQKIIVHHTAGRNNDPNPAATIRAIFYNHAVQRGYGDIDYNYLI
                     DAQGRVYEGRLGGAIGTGGPTAEDLAGNALRAAHALNFNDATMGVVLLGNFTSVMPTS
                     SARTALVNLLAWKAERHGMDPKGSSTYVNPVLGTTKFLKNISGHRDVNATACPGQLFY
                     NTFPQLRQDVADRIAATTGASHDHTPPGVIGQKTLGTNPSGAHTQRFGLIFTEPVTGL
                     APADFEVTGTSDGWTVDSVTGTASTYIVTVKADEGGGGPDDGTVILTLHAGGVTDKAA
                     HTGPPDDVSTTVAFAAETEPPTAILYAVAHGDEPSGTSYGISVLFDEPVTDFGPEDVQ
                     IGGTSNAATPWVLDRILGQDTMFNFTIHRDNPATGTLTVQVADGGVLDLAGNEGDGSN
                     VISKYVDHSAPTNTSPRTNLRADTTLNGGAMRVNLTWAGTDVGPAGIASYEVGRSYDG
                     KAFETIGTGVTSASFNWSTTPGHTYQFRVRAKDKAGNVGAWVTAPTVKPALTEQTSPW
                     VHWTGSTTTTSYSKYSGGSQRYLGAAGASATYTTTASSLSFVTTKGPNRGSAQVWIDD
                     VLAATVDLTTASTTYQVVAFARTWSTVASHKIKVVAVGGPIPRVDVDAFGVIR"
     gene            complement(6402..7436)
                     /locus_tag="VIF63_03520"
     CDS             complement(6402..7436)
                     /locus_tag="VIF63_03520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8238482.1"
                     /translation="MAMIGYAASMEQFHPNDLLDWCEQAEGAGFTAGFMVSEHFHPWT
                     PQQGQSGFAWSFMGALGLRTSLRFGTAVTCPGFRYHPAVIAHAAATLGAMFPGRFWLG
                     LGAGEALNEHVVGGEWPEIGVRSSMMFESIEMISELFTGKVVKKQGKHFKLESAKLYT
                     RPEEPVPIYVATSGPINAKKTGKFAQGMITVGAADEKVKLLWMACDEGCAEVGKAKIP
                     KLLQLHVSWAPTEEEALRNAMTEWPNGGMAFPKQDIKNPEDFAAMAKLVRPEDFKNRV
                     LISSDLEKHTAHVQHYLDMGFDEVHLHNVGRNQAEFIDVFGREVLPNLRLGITDDATP
                     MDPETIPAPA"
     gene            7487..8905
                     /locus_tag="VIF63_03525"
     CDS             7487..8905
                     /locus_tag="VIF63_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8238483.1"
                     /translation="MDLELFVTPGLGDASYLLASGGEAVLVDPQRDAWRFLEVAKRRG
                     WRVRYVLETHVHNDYLSGALETRAAAEAEIAAPARGRYEFEHLAVDEGDAIEIGSLRL
                     TAWATPGHTPEHLAWVVTDLARAIDAAEEPVGVFTGGSLLVGSAGRTDLLGPVLTDAL
                     TRDQQRTLHRLASLPDSAQVLPTHGAGSFCSAGPASSNRVSTIAAERLANPTFRLAAA
                     GAAPDVFRAQALDGLGRFPAYYAHMAGLNRRGPRVLGRLILPDALDPAAFEAAADGAT
                     VIDARDRESFAAGHLPGSLNIELDGTFAGYVGWLVPFASPVLFVLPDGAADALAEATT
                     ELLRIGYEWVRGWLAGGIDAWAASGRPLSSYETTTMEAVHDQRVAGDDASVLLDVRQP
                     MEWESDGVVPGAERIFVADLPARLAELPPDTPVTVFCRSGSRASIAASVLDRAGVEVK
                     LVAHGGASRWPEPLATLADAPA"
     gene            8902..9669
                     /locus_tag="VIF63_03530"
     CDS             8902..9669
                     /locus_tag="VIF63_03530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY8238484.1"
                     /translation="MKDDEGTVSGPDWWLRLNVAWWHLTIGPEYPPNEADKRDITLFG
                     LAFPRRASIAVFASTALIVVDQLGLLLASTTSAELPGGIRAVAVERFVLFLVVPVLII
                     MLGFRDDPRRYGLRLGNWRWGAGLLLVGLAVMTPILIALSGLDAFRDYYGTAPRPLGA
                     ALASNLVELIPAEFLLRGFLMFALWRRIGPLALIVAQVPFVLTHIGKPDVELWSTFIG
                     GSVFAWLDWRTGSILWSALGHVYVLTLMLVLVGGATR"
     gene            complement(9688..10173)
                     /locus_tag="VIF63_03535"
     CDS             complement(9688..10173)
                     /locus_tag="VIF63_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8238485.1"
                     /translation="MIPVAKLNHAVLYVRDAMVSAEFYGRLFGFEIVDNIGGRAVFMR
                     AGGGDNHHDLGLFSVGPNASRAPRGSTGLYHLAWEVPTIEDLATAARELRDAGALGGA
                     SDHGVSKSLYGADPDGNEFEIMWRVPRDAWGDAEKESGILPLDLDAEVRRWGAPGGPV
                     A"
     gene            10259..11410
                     /locus_tag="VIF63_03540"
     CDS             10259..11410
                     /locus_tag="VIF63_03540"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY8238486.1"
                     /translation="MKIGVARETASGERRVALVPDALAKITAAGVDVLVEQGAGVGAM
                     IPDSAYESAGAKVVSTGDLYEQADVILRVQKPSDDEAKRLRKGQAVIGTLQPLLDPKL
                     MAQLAEAGVTAISLDAIPRTLSRAQTMDALSSQANVGGYKAVLLAANAFGRYFPLLTT
                     AAGTAKPANVLILGIGVAGLQAIGTARRLGAVVKAYDVRPETREQAESLGAQFVTLKT
                     QIDATGAGGYARELTPEERASQQAELNEVIGAMDVVITTAQVPGRKPPVLVTTEAIGK
                     MKPGSVLIDMAASALGGNVELSKAGETIVTDNLVTIISPDNLPASMPIGASAFYARNI
                     SALLLQLLKDGALDLDPADEIAGPVTITHGGEIRSEPVRKLLAPAEAKA"
     gene            11407..11745
                     /locus_tag="VIF63_03545"
     CDS             11407..11745
                     /locus_tag="VIF63_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY8238487.1"
                     /translation="MSEALLAALTVFTLAILCGFAVISKVPATLHTPLMSGANSIHGI
                     VLVGSMVIAASATDVLSQVLSFVAVAFATMNVVGGYVVTDRMLQMFRRKPTAPKPDKA
                     DAGAAESAKG"
     gene            11748..>12243
                     /locus_tag="VIF63_03550"
     CDS             11748..>12243
                     /locus_tag="VIF63_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY8238488.1"
                     /translation="MSPETLALIESFVRLAWLIGAAGFVLGLMQMNSPATARTGNLVS
                     AGGMAIAIGGTAVLLAARGEAGIGAWIIILAGIAVGGGLGLYTARTVKMTAMPQLVSL
                     FNAVGGGAAALIAIDDYIRLARLQALLPNSIPLEISIPTVLDIVIGGITFTGSLIASG
                     KLMGI"
BASE COUNT         1752 a         4364 c         4235 g         1892 t
ORIGIN      
        1 catcatccgg gcgttcaggc cggcgacgag atagaaccag cccttcttgc ccagcagccg
       61 cccgatcacg tgggcgacgc cggcaaggat cattcgcggc gcgccggtga gctcgatcgc
      121 gtactgcatc gcgtacgggc tccggagccc ggtgccccac gtcgtcttga tgacccgcgc
      181 cgacatccag cgggcgaggc ggcccgggtg gatcgtgcgc cagtcgcggg cgcggccctg
      241 catgatcgcg acggctgact ccgacatgac gaccgtgtcg ccggcccgca ggaacggccc
      301 ggcatgctcc cggatgacgc cgggcatgtc attgccgggc atgaggacgg gtgtgcgaac
      361 cgggaggcgc tcgaagcttc ggccgtcgac gacgaccgtt cgcggcgcgg gcgcggcggg
      421 ggacatcgca ccgatggtac cggggggagt ctgtccggcc ggtggttgcg ggccccgacc
      481 cccgcgccta cgctcccgct cccatgtcgg ccccctcccc cgcggccaag ctcgtccgtc
      541 tggcctcggt gtcggcggcg ctggccctcc tcgccgccct catcgtccgg cccgcgctcg
      601 ggcccgtcgg cgcgcgagcc atgggcgagc tccccgactg tcgatacgac gacatcctca
      661 ccacgccgcg tggctatggc gactggtcga tcacgctcgt cgacacgatc ctccgactgc
      721 cgaagagcta cgccccgccg gatctcgtcc gcgtcgtcaa cctcggggtc ccgggccgcg
      781 gcaaggtccg tgccgtgatc gccgaggacc tcaaggcgat gagcgacgcg gccgcagcgg
      841 caaacgccgc gatcgccgtc tcgagcgcct atcgaagcta cgccgaccag caggccgtgt
      901 tcaggagctg ggtggcgagg tacggctaca agcgcgcgct ccagatctcg gcccggccgg
      961 gccactccga gcaccagctg ggcgtgacga tcgacttccg gagccagccc gacgacggcg
     1021 gcgagcttgc gacgtcctgg gcagggactc cggcaggcaa gtggatgaaa gcccacgcct
     1081 gggagtacgg cttcctcatg agctacccca agggcacgat ggccctgacc tgctacgact
     1141 gggagccgtg gcacttccgg tacgtcggcc gggagctcgc cgcgaagatc catgcgtccg
     1201 gtctcacccc gcgcgagtac ctctgggcgc acttcacgac gaccgtcgtg cccccgccgc
     1261 cgcccccgac gcacgccccc acgaagaccg ccaggcccag tgcggctccg accgccgctc
     1321 catcggttgc gccgtcaccg tcaccgacgg cactgccaac catcgcgccg gagaccatcg
     1381 ccccgccttc ggcgtcaccg gcgcccacgt cttcgcctgc gacgccggcc ggctccgcgc
     1441 ggccggtggt cccgcaggcc ccggccgccg ggttcgagcc ggcggtgctc gccggtggcg
     1501 tcgtcgtcct cggcacgacg gtcctgggcc ttctgttcgc cattcgtcgt gggcggtccg
     1561 gggcaggact gtagggctag agacactccc ggggaccgtc cagcaggatg cgcttccagc
     1621 gcgtggtcga tgcgctcagc gctcccagtt gctcatgagc ccactcgaac cgcgagggac
     1681 cgtcccatgc accgaacaca tcgagcgctg ctgcctttcc tcgtcgccgc gatcacggtg
     1741 ctcgtgatcc tgcccggatt cgcccagacg accgacgccg ccacgacgaa gtggaccgcg
     1801 aagtgctcga tcaacgtgcg cacggcgccc aagacctcgg cgacggtcaa gaaggtcatc
     1861 ctcaagggcg cgatcgtcac tgcgtcggcc accgtgaccg gcgggtcgtg gaaggcgtca
     1921 tgtggcggca cgaccgtgag cgggagctcg tggctgaaga tcgtggcgat caacggcaag
     1981 agcacgacgt cgctgttcgg ccggagcgct gtctatgccg cgaagggcct gttcaagtcc
     2041 ggtcccaacc cgaccgcgac gcccaagccg acggccgtcc ccaccgccaa gccgaccgca
     2101 gtcgtgacgg cggcaccgac cgccgcaccg acggcggcac cgacggcggc acctcccacg
     2161 accacgaact acatctcgaa ctgccccgtc cggctgcgcg cgacaccgtc ggtcaacgcc
     2221 gacacgatct cgatcatcga caccaacacg gtcgtcacca ccgcgaccaa ggtctccggt
     2281 ggcgcgtggg cggcggactg caagaccaac gtgtccggca cctcctggta caggatcacg
     2341 gccatcggcg ggaagtccgt ctcgtccctg tacggcgtga gcgtcgtgta cgcggcgacc
     2401 ggcctcttcc gatcggcctc gacctcgggc taccgcgagg gcatcgatgt ctcgcactgg
     2461 cagggcgcca tcgactgggc catggtcaag ggcgccggca agaccttcgc gatcgccaag
     2521 gcgaccgaag gaatcaattt caaggacgac cggtacgacc gcaacaaggc cgccgcgatg
     2581 ggccagggga tcaagttcgg cgcctatcac ttcgcccgac ctgagaacaa cgcggtcacc
     2641 gaagccgatt ggttcatcga caacgccggc tacgagcacg gcatgatcat cccgacgctc
     2701 gacctcgagc gaactggcgg cctcggcccg accgcgctca cgaactgggt caaggcgtgg
     2761 ctccagcgcg tcgaccagcg cctcggcgtt aagccgatga tctacatgag tccgtcgttc
     2821 tggcgttcga acctgaacga cagccgatgg ttcgccgaca acggctacgc gatcctgtgg
     2881 gtcgcccact ggggcgtgac gagtccgtcg atccccggcg cgaactgggg cggtcgatcg
     2941 tggaccttct ggcagtacac gagcagcggc accgtgcccg gcatcgccgg ccgggtcgac
     3001 ctcaaccggt atcgcttcgc gtccttcgac gccgtcacgt actgagccag gggcgcgccg
     3061 ccgcagcgtg gcgcaccgct agcatccccg gcgtgaccac cgtcagcgcg ggccggcccg
     3121 gacagctgct cgccctcgtg cccgcccatg acgaggcggc gggaatcggg accgtggtca
     3181 aaggcgcacg tcggcacgtg ccggtcctcg tcgtcgacga cggctccacc gatgacaccg
     3241 cagccctcgc cgaggccgcc ggcgcacgcg tgatccggca atcccccaac cagggcaagg
     3301 gcgccgccct gcgcgccggg ttcgcggcgg ccctcgccga gggcgcggaa gcggtcatca
     3361 cgctcgatgg cgacggccag cacgatccgt cggagatccc cgccttcctc ggcgcctacg
     3421 cccggcaggc gatcgccggc ggccaggccg agctcatcgt gggtcgccgc gacttccgcc
     3481 ggatgcccct cgttcgccgg ctcgcgaatg ggctggggac cctcgtgctg tcgggtgcgc
     3541 tcggtcgctg gatcccggac aaccagtccg gctaccgcct catcggacgg cgcctgatgc
     3601 aggcgacgct cgacagcgag gaggcgggct tcgcgttcga ggtcgagatg atcgcggtgt
     3661 gccagcgcga aggctggccg atcggctggg tgccggtccg cacgatctac gccggcgagt
     3721 cgagccacat ccggcccggc aagcacctcc gcgagttcct tgcggtcact cggcgcgcgc
     3781 gacggattgc ccggagccgg cccgtcagcg agtgacgctc agcgcctgac gctcagcgga
     3841 tgaccccgaa tgcgtcgacg tcgacgcggg ggatcggacc gcccacggcg acgaccttga
     3901 tcttgtgcga cgcgacggtc gaccaggtcc tggcaaacgc gacgacctgg taggtcgtgc
     3961 tcgcggtggt caggtcgacg gtcgccgcga ggacgtcgtc gatccagacc tgggcggagc
     4021 cgcggttcgg gcccttcgtg gtcacgaagg acagggagct ggcggtcgtc gtgtaggtcg
     4081 cgcttgcgcc cgccgcgccg agatagcgct gcgatccacc ggagtacttg ctgtacgagg
     4141 tcgtcgtggt cgacccggtc cagtgcaccc aggggctcgt ttgctcggtg agcgccggct
     4201 tgaccgtggg tgcggtgacc caggcgccga cgttgcccgc cttgtccttc gcccgcaccc
     4261 ggaactggta cgtgtggccc ggcgtggtcg accaattgaa cgacgcgctc gtcacgcccg
     4321 tgccgatcgt ctcgaacgcc ttgccgtcgt agctccgccc gacctcgtac gaggcgatgc
     4381 cggccggccc gacatcggtg ccggcccagg tgaggttcac ccgcattgcg ccgccgttca
     4441 gcgtcgtgtc cgctcgcagg ttcgtcctcg gtgaggtgtt ggtcggcgcc gagtggtcga
     4501 cgtacttcga gatcacgttg gagccgtcgc cctcgttgcc ggcaaggtcg agcacgccac
     4561 cgtccgcgac ctggacggtg agggttccgg tcgcggggtt gtcgcggtgg atcgtgaagt
     4621 tgaacatcgt gtcctggccg agaatccggt cgagcaccca cggcgtcgcg gcgttggacg
     4681 tcccgccgat ctggacgtcc tccggcccga agtccgtgac cggctcgtcg aagagcacgg
     4741 agattccgta gctggtgccg ctcggctcat cgccgtgcgc aacggcgtag aggattgccg
     4801 tcggcggctc ggtctcggcc gcgaaggcga ccgtcgtcga gacgtcgtcg ggtggtccgg
     4861 tatgggcggc cttgtcggtc acgccgccgg cgtggagggt gaggatcacc gtcccgtcgt
     4921 ccggcccgcc gccgccctcg tctgccttca cggtcacgat gtacgtcgag gcggtgccgg
     4981 tgacgctgtc gaccgtccag ccgtccgatg tcccggtgac ctcgaaatcc gccggtgcga
     5041 ggccggtgac gggctcggtg aagatcagcc cgaaccgctg ggtgtgcgca ccgctcggat
     5101 tcgtgcccag ggtcttctgg ccgatcacgc cgggcggggt gtggtcgtgg ctcgcacccg
     5161 tcgtcgccgc gatgcggtcg gcgacgtcct gccggagctg ggggaacgtg ttgtagaaga
     5221 gctggccggg acaggccgtg gcgttgacgt cgcgatggcc ggagatgttc ttcaggaact
     5281 tggtcgtgcc gaggaccggg ttgacgtagg tcgaggagcc cttcgggtcc atcccgtgac
     5341 gctccgcctt ccaggcgagc aggttcacga gggcggtgcg agccgatgag gtcggcatca
     5401 cgctcgtgaa gttgccgagg aggacgacgc ccatggtcgc gtcgttgaag ttcagggcgt
     5461 gggcagcgcg gagggcgttg ccggcgagat cctcggcggt cggcccgccg gtcccgatgg
     5521 cgccaccgag ccggccctcg tacacgcggc cctgcgcgtc gatgaggtag ttgtagtcga
     5581 tgtcgccgta gccacgttgg actgcgtggt tgtagaagat cgcgcggatc gtcgccgccg
     5641 ggttggggtc gttgttccgg cccgcggtgt ggtggacgat gatcttctgg agcggggtgt
     5701 agttcggcgc gaatcgaacg tagccgccgg cgttgtgcga gtagtcctcg ttggcgcccc
     5761 agcctgcccg agtgatgacg gcaggctggt tgaccgccgc cagcacgacg ccgctctggt
     5821 cgatgccgcg cgaggcgtcg gcgtcgatcg ccgacacggt caggtgggcg atcggccggt
     5881 ccgaggtgac ccgcgcatat cgcgcgccgc ccgcccagac gacctcgctc cacgaatcga
     5941 cagccgccgg atccgcgtcc tcatcggcgc cgagcgggat ctcctcgctc agctggtcgg
     6001 gcgttcgccc gaccgcgatc gagaggacgg cctcgggcgc gccggtccaa tgcaggacca
     6061 cgtgggatgc cgcgatcggc aggtgaacga gctgctggcg atccaccgcg acctcgacgt
     6121 cgcgacgcac gatgtcgacc ccgcggtgca cggctgactg gaggatcggc agcccgccga
     6181 cggcgagtgc ggcgaggagg aggccggccg tcaaacggcg aacggtgagt cggcgtgctc
     6241 ggtgcatggt cgcgggcctc gctggggacg ccacgacccc tgctccgtgg cggcgcaagc
     6301 ctagtcccgc gacgggccgg aggacagtag ccaagccgac ccacgccggg agcgggacta
     6361 tcggacgctc cggccggctg gctttcggaa ccggaccgcg atcaggcagg cgccggaatc
     6421 gtctccgggt ccatcggtgt cgcatcgtcg gtgatgccca gccgcaggtt cggcagcacc
     6481 tcgcgcccga acacgtcgat gaactcggcc tggttccggc cgacgttgtg gaggtgcacc
     6541 tcgtcgaagc ccatgtcgag gtagtgctgg acgtgggcgg tgtgcttctc gaggtcggac
     6601 gagatcagga cgcgattctt gaagtcctcg ggccggacga gcttcgccat cgcggcgaag
     6661 tcctcggggt tcttgatgtc ctgcttcggg aaggccatgc cgccgttcgg ccactccgtc
     6721 atggcgttcc ggagcgcttc ctcctcggtg ggcgcccatg agacgtgcag ctggaggagc
     6781 ttcgggatct tcgccttgcc gacctcggcg cagccctcgt cgcaggccat ccacaggagc
     6841 ttgaccttct cgtcggccgc gccgacggtg atcatcccct gggcgaactt gccggtcttc
     6901 ttggcgttga tcggcccgga ggtcgcgacg tagatcggga cgggctcctc ggggcgcgtg
     6961 tagagcttcg cgctctcgag cttgaagtgc ttgccctgct tcttgacgac cttgccggtg
     7021 aacagctccg agatcatctc gatcgactcg aacatcatcg acgaccgaac gccgatctcc
     7081 ggccactcgc cgccgaccac gtgctcgttg agcgcctcgc cggcgcccag gcccagccag
     7141 aaccgaccgg gaaacatcgc gccgagcgtc gccgcggcat gggcgatcac cgccgggtgg
     7201 tagcggaagc cgggacacgt caccgcggtc ccgaaccgga gcgacgttcg cagcccgagc
     7261 gcgcccatga acgaccacgc gaagcctgac tggccctgct gcggcgtcca cgggtggaag
     7321 tgctccgaga ccatgaagcc ggccgtgaag cccgcgccct ccgcctgctc gcaccagtcg
     7381 agcaggtcgt tcgggtggaa ctgttccatc gaagccgcgt agccgatcat cgccatggcc
     7441 gcacatggta cgagccccgg gctcgagcgc cgtacgatga cggccgatgg acctcgagct
     7501 cttcgtcacg cccggcctgg gcgacgcctc gtacctgctc gcgagtggcg gcgaggccgt
     7561 cctcgtcgat ccccagcggg atgcgtggcg gttcctcgag gtcgcgaagc gtcgcggctg
     7621 gcgggtccgg tacgtgctcg agacgcatgt ccacaacgac tacctctcgg gggcgctcga
     7681 gacgcgggcc gcggcggagg ccgagatcgc cgcgccggcg cgcggccgct acgagttcga
     7741 gcacctggcg gtcgacgagg gcgacgcgat cgagatcggg tcgctgcggc tcacggcctg
     7801 ggcgacgccc gggcacaccc cggagcatct cgcctgggtc gtcacggatc tcgcgcgcgc
     7861 gatcgacgct gccgaggagc cggtcggcgt cttcacgggc gggagcctgt tggtgggttc
     7921 cgccggacgc accgacctgc tcgggcccgt cctgaccgac gccctgacgc gtgatcagca
     7981 gcggaccctg catcgcctcg cctcgctgcc cgacagcgcc caggtcctgc cgacgcacgg
     8041 cgccggcagc ttctgcagcg ctggtccagc gagctcgaat cgcgtctcga ccattgccgc
     8101 cgaacgcctc gccaacccga cgttcaggct cgccgccgcc ggtgcggctc cggacgtctt
     8161 ccgagcgcag gcgctcgacg gcctcggccg cttccccgcc tactacgcgc acatggccgg
     8221 gctcaaccgg cgtggcccgc gggtccttgg ccggctgatc ctgccggacg cgctcgaccc
     8281 ggcggccttc gaagctgctg ccgacggcgc gacggtcatc gatgctcgcg atcgcgagtc
     8341 gttcgccgcc ggccacctgc ccggctcgct gaacatcgag ctggacggga cgttcgcggg
     8401 ctacgtcggc tggctcgtgc cgttcgcgtc gcccgtcctg ttcgtcctcc ccgatggtgc
     8461 cgccgacgcc ctcgccgagg caacgacgga gctcctccgc attggctatg agtgggttcg
     8521 cggctggctc gccggcggga tcgacgcctg ggccgcgtcc ggccggccgc tctcgagcta
     8581 cgagacgacg acgatggagg ccgtccacga ccagcgcgtc gccggcgacg atgcgagtgt
     8641 gctcctcgac gtccgccagc cgatggagtg ggaatcggac ggggtcgtgc ccggggcgga
     8701 gcggatcttc gtcgccgacc tgccggctcg cctcgccgag ctgccgccgg acacgccggt
     8761 gacggtgttc tgccgctccg gctcgcgcgc gtcgatcgcg gcgagcgtgc tggatcgcgc
     8821 aggcgtggag gtcaagctcg tcgcccacgg cggcgcctcg cgctggcccg agccgctcgc
     8881 gaccctcgcc gacgccccgg cgtgaaggac gacgaaggca cggtcagcgg cccggactgg
     8941 tggctccggc tcaacgtcgc atggtggcac ctgaccatcg ggcctgaata cccgcccaac
     9001 gaggcggaca agcgcgacat cacgctcttc gggctcgcgt tcccgcggcg cgcctcgatt
     9061 gccgtcttcg cctcgaccgc cctgatcgtc gtcgaccagc tcggcctcct gctcgcgtcg
     9121 acgacgagcg ccgagctccc cggcggcata cgcgcggtgg ccgtcgaacg gttcgtgctg
     9181 ttcctggtcg tgcccgtgct gatcatcatg ctcggcttcc gtgacgaccc tcggcggtac
     9241 gggctccgcc tcggcaactg gcgctggggt gcgggcctgc tgctcgtcgg tctcgcggtc
     9301 atgaccccga tcctcatcgc gctctcgggg ctcgatgcat ttcgcgacta ctacggcact
     9361 gccccgcgcc cgctcggcgc cgcgctggca agcaacctcg tcgagctcat cccggccgaa
     9421 ttcctgctcc gcggcttcct gatgttcgcg ctgtggcggc gaatcgggcc gctggcgctg
     9481 atcgtcgcgc aggttccgtt cgtgctgacc cacatcggca agccggacgt cgagctgtgg
     9541 tcgacgttca tcgggggctc ggtgttcgcc tggctcgact ggcggacggg ctcgatcctg
     9601 tggagcgccc tcgggcacgt gtacgtgctg acgctgatgc tcgtcctcgt cggcggcgcg
     9661 acccgctaga ccccgcgtcc aatgccgcta ggcgaccggg ccgcccggtg ctccccagcg
     9721 acggacttcg gcgtcgaggt cgagcggcag gatcccgctc tccttctcgg cgtcgcccca
     9781 cgcgtcgcgc gggacacgcc acatgatctc gaactcgttg ccgtcggggt ccgcgccgta
     9841 gagcgacttc gacacgccgt ggtcactcgc gccaccgagg gcgcctgcgt cccgaagctc
     9901 gcgtgccgcg gtcgccaggt cctcgatcgt cgggacctcc caggcgaggt ggtacaggcc
     9961 ggtcgaaccg cgcggggcgc gcgatgcatt cggcccgacc gagaacagcc cgagatcgtg
    10021 gtggttgtcg ccaccgccgg cgcgcatgaa cacggcccgc ccgccgatgt tgtcgacgat
    10081 ctcgaagccg aacaagcggc cgtagaactc ggcggagacc atggcgtcgc ggacgtacag
    10141 gaccgcgtgg ttgagcttgg cgacggggat catggggcag gctcctgaac tggttgttgc
    10201 cgcaactata tcaccggcct acgcccagcc cacgcgaccc gatagacttc cggctcgcat
    10261 gaagatcggt gtcgcacgag aaaccgcgtc cggggagcgt cgcgtcgcgc tcgtgccgga
    10321 cgcgctcgcc aagatcacgg cggcgggcgt cgacgtgctc gtcgagcagg gcgccggcgt
    10381 cggggcgatg atcccggact cggcgtacga atcggccggc gcgaaggtcg tctcgaccgg
    10441 cgacctgtac gagcaggcag acgtgatcct ccgcgtccag aagccgagcg atgacgaggc
    10501 gaagcgactg cgcaaggggc aggcggtcat cggcaccctc cagcctctcc tcgatccgaa
    10561 gctcatggcg cagctcgccg aagcgggcgt cacggccatc tcgctggacg ccatcccgcg
    10621 gacgctctcg cgcgcccaga cgatggacgc cctctcgtcg caggcgaacg tcggcggcta
    10681 taaggcggtc ctcctcgccg cgaacgcctt cgggcggtat ttcccgctgc tcaccaccgc
    10741 cgccggcacg gccaagcccg cgaacgtgct catcctcggc atcggcgtcg ccgggctcca
    10801 ggcgatcggc acggcgcgcc ggctcggggc cgtcgtgaag gcgtacgacg tccgccccga
    10861 gacgcgcgag caggcggaat cgctcggcgc gcagttcgtc acgctgaaga cccagatcga
    10921 tgccaccggc gcgggcgggt acgcccgcga gctcacgccc gaggagcgcg cctcgcagca
    10981 ggcggagctg aacgaggtga tcggcgccat ggacgtcgtc atcaccacgg cccaggtccc
    11041 gggccgcaag ccgccggtcc tcgtcaccac cgaagcgatc gggaagatga agcccggctc
    11101 ggtgctcatc gacatggcgg catcggcgct cggcgggaac gtggagctgt cgaaggccgg
    11161 cgagacgatc gtgaccgaca acctcgtaac aatcatctcg cccgacaacc tgcccgcctc
    11221 gatgcccatc ggcgcatcgg cgttctacgc gcgaaacatc agcgccttgc tgctgcagct
    11281 gctcaaggac ggcgcgctcg acctcgaccc ggccgacgag atcgccggtc ccgtgacgat
    11341 cacccacggc ggcgagattc gcagcgaacc ggtcaggaag ctcctcgcgc ccgcggaggc
    11401 caaggcatga gcgaagcgct gctcgcggcc ctgaccgtct tcacgctcgc gatcctgtgc
    11461 ggcttcgcgg tcatctcgaa ggtgcccgcg accctgcaca cgccgctgat gtcgggcgcc
    11521 aactcgatcc acggcatcgt gctcgttggt tcgatggtca tcgccgcctc ggcgacggac
    11581 gtgctgagcc aggtcctgag cttcgtggcg gtcgcgttcg cgacgatgaa cgtggtcggc
    11641 ggttacgtcg ttaccgaccg gatgctccag atgttccggc gcaagccaac cgcgccgaag
    11701 cccgacaagg ctgacgcggg cgccgccgag agcgcaaagg gctgacgatg agccccgaga
    11761 cgctcgccct gatcgagtcc ttcgtccgcc tcgcctggct gatcggcgcg gctgggttcg
    11821 tgctcggcct gatgcagatg aactcaccgg cgacggcgcg caccggcaac ctcgtttcgg
    11881 ccggcggcat ggcgatcgcg atcggcggca cagccgtcct gctcgcggcg cgcggcgagg
    11941 ccgggatcgg cgcctggatc atcatcctcg ccggaatcgc ggtcggcggc gggctcgggc
    12001 tgtacaccgc gcggaccgtg aagatgaccg caatgcccca gctggtgtcg ctgttcaacg
    12061 cggtcggcgg cggcgcggcg gccctcatcg cgatcgacga ctacatccgg ctcgcgaggc
    12121 tccaggcgct gctgccgaac tcgatcccgc tcgagatctc gatcccgacc gtcctcgaca
    12181 tcgtgatcgg cggcatcacc ttcaccggct cgctcatcgc atccggcaag ctcatgggca
    12241 tcg
//
LOCUS       DATBSW010000127         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_221475, whole genome shotgun sequence.
ACCESSION   DATBSW010000127 DATBSW010000000
VERSION     DATBSW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221475"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIF63_03555"
     CDS             complement(<1..203)
                     /locus_tag="VIF63_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238489.1"
                     /translation="MTDITESELTEAEMTLAPDAAPEEDVDEAVEVDGSAIGTDVTDG
                     ESQAADVDGGADSTDATAERDEL"
     gene            complement(308..799)
                     /locus_tag="VIF63_03560"
     CDS             complement(308..799)
                     /locus_tag="VIF63_03560"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY8238490.1"
                     /translation="MSGEVSFDVVSEFDAMELRNALDQVRRETAQRYDFRGATVDLEQ
                     GKDELVLVTDDEYKATAVKDLILSKAVRRGLSLKIFDFGAVEPVGGQKVRQKITLRSG
                     LPEDLAKRIQKLIRDEFPKVKSQIQGDAVRVSGKSKDDLQRVITRLRGLDEVVELQFQ
                     NYR"
     gene            complement(893..2386)
                     /locus_tag="VIF63_03565"
     CDS             complement(893..2386)
                     /locus_tag="VIF63_03565"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8238491.1"
                     /translation="MTRTAAELGEGLAPVRAARAIRPGERIHILGIAGAGASAAALHA
                     HAAGAAVSGCDPGGPSPYTPALEAAGIPIAWGHDAAHVAVDPAPDRLAVTKALTAIDP
                     HNAELVAAATQGIPIEGWQQLVADAATGRTLVAVAGTHGKSTTAGWLTWVLAEAGLDP
                     SAFVGALLPAVLTGDLPATARVGQGSPFVVEADEYAGNFDPYRPDVALVTTVEWDHPD
                     VFANRAAVLQAFAAWLGQVPEATLVANVADRGVRDLLALLGKRQGHIVRVAVGNADDA
                     PDMDLRAEISAWSNAGTVIELSGSGVASRSIQLRTSGGHNVVNALVVAAAAQALGADP
                     EHIASGLSTFPGIGRRMERKGEAGGVVVYDDYGHHPTAVRATIAAMRQREPGRRLWAV
                     YEPLTFHRTAAMLDAFAAALAEADAVAVADIWASRDPDTTITSPEALAAAVLERRPEI
                     EAAAPGSVEQTAGWLAAHVRPGDAVLVMGGGRSYRIAELLLENLERS"
     gene            complement(2383..>2900)
                     /locus_tag="VIF63_03570"
     CDS             complement(2383..>2900)
                     /locus_tag="VIF63_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEY8238492.1"
                     /translation="DAADVGVPPAALVALAERLQGLCLGRGLTVATAESCTGGLVATT
                     ITEVAGSSGYFLGGVVSYADTVKASLLGVPEATLAAHGAVSAQVAVAMATGARARFAS
                     ALAVSITGVAGPDGGSEAKPVGLTYIGLAADAGTEVRRFAFTGDRAGNREAAARAALE
                     WLIERAEAGAA"
BASE COUNT          411 a         1054 c         1007 g          428 t
ORIGIN      
        1 tggagctcgt cgcgttcggc cgttgcatcc gtcgagtcgg cgccaccgtc gacgtcggca
       61 gcctgcgact cgccatcggt aacgtcggtt ccgatcgcgc tcccgtcgac ctcgaccgcc
      121 tcgtccacgt cctcttccgg cgctgcgtcg ggggcgagcg tcatctcggc ctcggtgagc
      181 tcggactcgg tgatatcggt catcagaagg tcggcccggg gatcgaggtg agcacgaggc
      241 tcaagacgag catgatcacg acgagcacga cgaggatgcg cgcgacgatc cggccggcca
      301 tgcgtcgcta ccggtagttc tggaactgga gctcgacgac ttcgtcgagc ccgcggaggc
      361 gcgtgatcac gcgctggagg tcgtccttgc tcttgcccga gacccgcacg gcatcaccct
      421 ggatctggga cttgaccttg gggaactcgt cgcggatcag cttctggatc cgcttcgcga
      481 gatcctcggg cagcccgctt cgcagcgtga tcttctggcg gaccttctgg ccacccaccg
      541 gctcgacggc cccgaagtcg aagatcttga gcgacagccc acgccggacg gccttcgaca
      601 ggatcaggtc cttgactgcc gtggccttgt actcgtcgtc ggtcacgagg acgagctcat
      661 ccttgccctg ctcgaggtcg acggtggcgc cccggaagtc gtagcgctgc gccgtctcgc
      721 gccggacctg gtccagcgcg ttgcgcagct ccatggcatc gaactcgctg acgacgtcga
      781 acgagacctc gccactcacc gtcaatcgcc tcctgggccg gcgcggctac tgcccacacc
      841 ggagcttcgt ggatgtcggg cgccagtatg ccggttcgag cgcaaacggc aatcaggacc
      901 tttcgagatt ctcgaggagc agctcggcga ttcggtagct tctgccgcca cccatcacca
      961 gcacggcgtc acccggccga acgtgtgcgg cgagccagcc ggccgtctgc tcgaccgatc
     1021 ccggcgccgc ggcctcgatc tccgggcggc gctcgagaac ggcagcggca agcgcttcgg
     1081 gcgacgtgat cgtcgtgtcc gggtcgcgac tcgcccagat gtcggcgacc gccacggcat
     1141 cggcctcggc gagggccgct gcgaacgcgt cgagcattgc tgccgtccgg tggaacgtga
     1201 gcggctcgta gacggcccac agccgccggc cgggctcccg ctggcgcatg gccgcgatcg
     1261 tggctcgaac ggccgttgga tggtgcccgt agtcgtcgta gaccacgacc cctccggcct
     1321 cgcccttgcg ttccatccgc cggccgatgc ccgggaacgt cgacaggccc gatgcgatgt
     1381 gctccggatc ggcgccgagc gcctgcgcag cagccgcaac cacgagggcg ttcacgacgt
     1441 tgtggccccc cgacgtccgc agctggatcg accgggaagc cacgcccgac cccgacagct
     1501 cgatgaccgt ccctgcgttg ctccaagcgc tgatctccgc ccggaggtcc atgtcggggg
     1561 cgtcgtcagc gttcccgacc gcgaccctga cgatgtggcc ctggcgcttc cccagcagtg
     1621 caaggaggtc gcggacgccc cggtcagcga cgttcgcgac caacgtcgcc tccgggacct
     1681 gtcccagcca cgcggcgaac gcctggagca cggcagcgcg attcgcgaag acatcagggt
     1741 gatcccattc gaccgtcgtc acgagggcga cgtcggggcg gtatgggtcg aagttgccgg
     1801 cgtactcgtc ggcctcgacg acgaacgggc tcccctgccc cactcgcgcc gtggccggca
     1861 ggtcgcctgt gaggacggca gggagcagcg caccgacgaa cgccgagggg tcgagcccgg
     1921 cctcggcgag gacccaggtc agccagccgg cggtggtgct cttgccgtgg gtgccggcga
     1981 cggcgacgag ggtgcgcccg gtcgcggcgt cggcgacgag ctgctgccag ccttcgatgg
     2041 ggatgccctg cgtggcggcc gcgaccagct cggcgttgtg cgggtcgatc gcggtcaggg
     2101 ccttggtgac ggcgagccgg tccggtgccg ggtcgacggc aacgtgcgca gcatcgtgcc
     2161 cccacgcgat gggaatgccc gccgcctcga gggcgggcgt gtacggcgaa gggccgccgg
     2221 gatcgcagcc ggacacggcc gcacccgcgg catgggcgtg gagcgcagcg gcggacgcgc
     2281 ccgcgcccgc aatcccgagg atgtggatgc gttcgccggg gcgaatggcg cgcgccgcgc
     2341 gaacgggcgc gaggccctcg ccgagctccg ccgcggttcg ggtcacgcgg ctcctgcttc
     2401 cgcccgctcg atgagccact ccagcgcagc gcgagctgcc gcttcgcgat tgcctgcccg
     2461 gtcgccggtg aacgcgaacc gtcgcacctc cgtgccggca tcggcggcca ggccgatgta
     2521 cgtcagtccc actggcttcg cctcggaacc accgtccgga ccggcgacgc ccgtgatcga
     2581 taccgccagg gcagacgcga agcgggcacg cgccccggtc gccatcgcga ccgcaacctg
     2641 cgcgctgacg gccccgtggg cggcgagcgt cgcctcgggc acgccgagca gcgacgcctt
     2701 caccgtatct gcgtaggaca ccacgccacc caggaaatag ccggaggagc ccgcgacctc
     2761 cgtgatggtc gtcgcgacca gcccgccggt gcacgactcg gccgtcgcga cggtcagccc
     2821 tcgcccgagg cacaggccct gcagccgctc cgccagggcg acgagcgctg ccggcggcac
     2881 gcctacgtcc gctgcgtctt
//
LOCUS       DATBSW010000128         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_679938, whole genome shotgun sequence.
ACCESSION   DATBSW010000128 DATBSW010000000
VERSION     DATBSW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679938"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VIF63_03575"
     CDS             <1..613
                     /locus_tag="VIF63_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8238493.1"
                     /translation="KLMEVARQLNYDSIDDFYAAIGYGAVSPQSVVMKLGVVDDKELA
                     LPTVAPPPSPARAGGVRVKGVGDLLVRFARCCHPIPGDPIEGFITRGKGVTVHLRSCP
                     TVLNEREISRLIDVEWEAAPTQTYPIAIRVEAYDRTGLLSDITQVVAEAKVNILAADV
                     SVGPDHQAIVRATLQVASVSQLSRVLSRIEQLKDVSSVQRDLG"
     gene            662..2005
                     /gene="hisS"
                     /locus_tag="VIF63_03580"
     CDS             662..2005
                     /gene="hisS"
                     /locus_tag="VIF63_03580"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8238494.1"
                     /translation="MPQFRAPRGTRDLLPPERVGLARLERLGAELAARYGYRLIETPL
                     FEQSAVFERGIGEVTDVVEKELFLLAPREEDGERWALRPEPTAGIVRAYVQHGMQTWP
                     QPVKVTMIGPMFRYDRPQKGRYRAFWQFDVEAIGDPGPAVDAEIIELAARFYRDAGLV
                     DVTIYLNSIGDAACRPAYVEELAAYYRSHISELPTLERARLDRAPLRLLDSKDERVVA
                     LNAAAPKITDRLCDACAAHFAGVRAHLDAVGVAYTLEPGLVRGLDYYTRTAFEVYPAG
                     ATGQQSAIGGGGRYDGLVELLGGRPTPGIGFGLGLDRVLRALEDAGAPPVAEPTPVAV
                     VVGSDPADTVTRLRIATDLRGAGLAVRAELGQRKLGKQLEAAARDGAHFAVILGDELS
                     GGHVQLRDLEAGTQRAVGVANLARELERATKSHKHGLAAGSSPALGLASKPAAEN"
     gene            complement(2408..>2633)
                     /locus_tag="VIF63_03585"
     CDS             complement(2408..>2633)
                     /locus_tag="VIF63_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238495.1"
                     /translation="DVRRDPPFVSAVSVYDVGGRVVRQPFTARVLSEAELATELDAAG
                     LIVRQRLSPTWLLAGHGGAGLSSPSHLFSR"
BASE COUNT          399 a          919 c          928 g          387 t
ORIGIN      
        1 caagctgatg gaggtcgcgc ggcagctcaa ctacgacagc atcgacgact tctacgccgc
       61 gatcggctac ggcgcggtga gcccgcagtc ggtcgtcatg aagctcggcg tcgtcgacga
      121 caaggagctg gcgctcccga cggttgcgcc gccgccgtca ccggcgcgcg ccggcggggt
      181 ccgggtcaag ggggtggggg acctcctcgt tcggttcgcg cgctgctgcc acccgatccc
      241 gggcgacccg atcgagggct tcatcactcg cggcaagggc gtgacggtgc acctccgatc
      301 ctgcccgacg gtgctcaacg agcgcgagat ctcgcgcctg atcgacgtcg agtgggaggc
      361 cgcgccgacg cagacctacc cgatcgcgat ccgcgtcgag gcgtacgacc gaacgggcct
      421 gctcagcgac atcacccagg tcgtcgcgga ggcgaaggtc aacatcctcg ccgccgacgt
      481 gtcggtcggg cccgatcacc aggcgatcgt gcgcgccacg ctgcaggtcg cgtcggtcag
      541 ccagctgtcg cgtgtcctct cgcgcatcga acagctcaag gacgtctcct cggtccagcg
      601 cgacctcggg tagggtctcc ccgcggtcga acgggcgtcg gccgccgcta gactttcgcg
      661 gatgccgcag ttccgagcac cccgaggcac ccgcgacctc ctgccgcccg aacgggtggg
      721 ccttgcgcgc ctggagcgcc tcggcgccga gctcgcggcg cggtacggct accgcctgat
      781 cgagacgccg ctgttcgagc agtcggccgt cttcgagcgg ggcatcggcg aggtcaccga
      841 cgtcgtcgag aaggagctct tcctgctcgc gccgcgcgag gaggacggcg agcgctgggc
      901 gctccgcccg gaaccgacgg cgggcatcgt tcgtgcgtac gtccagcacg ggatgcagac
      961 gtggccgcag ccggtcaagg tcacgatgat cggcccgatg ttccggtatg accgcccgca
     1021 gaagggccgg taccgggcgt tctggcagtt cgatgtcgag gcgatcggcg accccgggcc
     1081 ggcggtggac gccgagatca tcgagctcgc ggcgcgtttc tatcgcgacg cggggctggt
     1141 cgacgtgacg atctacctga actcgatcgg ggacgcggcc tgccggccgg cgtatgtcga
     1201 ggagctcgcg gcctactacc ggtcacatat ctctgagctc ccgaccctcg agcgggcgcg
     1261 cctggaccga gcaccgctgc ggctgctcga ctccaaagac gagcgggtcg tcgcgttaaa
     1321 cgcggctgcg ccgaagatca cggatcggct gtgcgacgca tgtgccgcgc atttcgcggg
     1381 agtccgcgcg cacctcgacg cggtaggcgt ggcgtacacg ttggagccgg gcctggtgcg
     1441 cgggctcgac tactacacgc gaaccgcgtt cgaggtctat cccgccggcg ccacgggcca
     1501 gcagtccgcc atcggtggtg gcggccggta cgacggcctg gtcgagctgc tcggcggccg
     1561 accgacgccg gggatcggct tcgggttggg gctcgatcgc gtacttcgag cgctcgagga
     1621 cgccggcgcg ccgccggtcg ccgagccgac gcccgtcgcc gtcgtggtcg gcagcgaccc
     1681 cgccgacacg gtgactcgtc tgcgaatcgc caccgacctg cgtggcgccg ggcttgcagt
     1741 tcgcgccgag ctgggccagc gcaagctcgg caagcagctc gaggccgcgg cgcgcgatgg
     1801 cgcccacttc gcggtcatcc tcggcgacga gctgagcggc ggccacgtcc agctgcgtga
     1861 cctcgaagcg ggtacccagc gcgccgtggg cgtcgcgaat ctcgcccgcg agctcgagcg
     1921 cgcaacgaag tcacataagc acgggctcgc cgccgggtca tctccagccc tcggcctggc
     1981 ctccaagccc gccgccgaga actaggcgcg gacgaacccg ggacggcccg gacggacccg
     2041 gaactgaccc ggagctgctg tcagcggacg gcacgcatgg ccgccacaac ttctcccccg
     2101 ggccgccgtt tggagatagt tccaaccaaa cggaaccatc gccgaaagcg ccaattcggt
     2161 ggcagctcgt gctggggcag cggcgagcct cgaaattgat cgggaaacgc ggtgccacca
     2221 acagggttcg atgcggaatc ggcgccggcc cgactgcttt gggaccgccg caggccttcg
     2281 cggatcggtc ccgactcact tcgcgggagg ctccgatccc gttttcccga tcaaagctgg
     2341 gtcatcccgg cttcgacacg cgcgtccgat tcgggctttt gacgacgatg gttctgcttc
     2401 attcgaacta tctcgaaaac aggtggctgg gcgatgaaag cccggcaccg ccatggccgg
     2461 cgagtagcca cgtcgggctc aggcgttggc gaacgatcaa gcccgcggcg tccagctcgg
     2521 tggcgagctc cgcttccgac agcactcgcg cggtgaacgg ctggcgcacg acgcggccgc
     2581 cgacgtcgta cacggagact gccgagacga atggcgggtc gcggcggacg tcg
//
LOCUS       DATBSW010000129         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_715073, whole genome shotgun sequence.
ACCESSION   DATBSW010000129 DATBSW010000000
VERSION     DATBSW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715073"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3379)
                     /locus_tag="VIF63_03590"
     CDS             complement(<1..3379)
                     /locus_tag="VIF63_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY8238496.1"
                     /translation="MAEADGREQIAIIGMAGRFPGAVDVDQFWANLRNGVESIRAFTD
                     AELSEAGADTSEPGFVNAGSVMDGIDMFDAGFFGMSRREAELTDPQHRVFLECAWTAI
                     EHAGYEPGADVGRIGVFGGVGPNTYFRRNVVDHADLLARTGDYPLLLATEREYAITRV
                     AYKLGLRGPAISLNTACSTGAVAVHLAVQSLLSGESDLALAGACRIRVPATAGYVYQE
                     DGIPSPDGHCRAFDADARGTVLASGAAVVVLKRLSDAIRDDDTVYAVIKGSAVNNDGA
                     AKIGYTAPSIEGQTAVIEEALAVAEVDADTIGMVEAHGTGTSLGDPIEVAALTQAYRR
                     DTSRRQYCAIGSLKSNIGHLDAAAGVAGIIKAALSLHHEEIPPSLNFNRPNPQIDFSG
                     SPFFVNTELRAWKRTEQPRRAAVSAFGLGGTNAHLVLEEAPERAPAPAGPRKAHQIVT
                     LSAKSHAALEAMGGALADHLERHPDLELADVGYSSQLGRARMPHRRSFVASTVREAMR
                     LLREPDPLVVSDRVTASDGARVAFMFPGQGAQHPGMGAGLYQSEPVFKAALDECARIL
                     APILGRDLLEVIYPEADKVDAAAEELGDAALAQPATFAIQYALARLWMSWGVQPSAMV
                     GHSLGEFAAACLGGVFSLQDALRLVAERGRLMQAQLGGGMLAIMLEPEAVIPLLDAAT
                     SLAAINAPAQCVASGPEASISRLEEQLVAADVEVQRLPIALAAHSPMMDPIVAQFREL
                     VRGTARGPLTVPIVSTVTGTWAAEDQLADPDYWATHLQQTVRFVDAANRLLDQPDMIL
                     LEVGPGQTLASLVRQYPEAMPDRVVLSSLRHRRQQVSDEEWILRALGQVWGAGGDVDW
                     PGVHGGRRRRVVLPTYPFDHERYWIDPIGRSRGAASAPRPTRAAPPDPEPVHQEATVA
                     RTENLVADGPEPATELRKDRIIVRLASILSELSGVDPSALDPSASFADLGFDSLFLTQ
                     ANAQFRKQFGVRITFRQIFEEAPSIDSLAGFIDGKLAPEAFPGPQRPVVTSPTPADAA
                     GHRDDSGPGVRELHGVETEPASTTAVERLIREQLRVMERQIELMRSGDRSDAVAPALM
                     APTPSVDAPAPFVEAPAPSSPRTHYLATRDQGHRPAGN"
     gene            complement(3372..>4729)
                     /locus_tag="VIF63_03595"
     CDS             complement(3372..>4729)
                     /locus_tag="VIF63_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8238497.1"
                     /translation="ARRELTTPYAAPRSKVERLVADMWRAILEIDTVGVHDRFFELGG
                     TSLQAARFVNELQTELGESIFVVTLFAAPSVAEYATFLQEQYPAAVARLVGRDHALVR
                     PASSTAVISAADVARLRAAVPIADAIVAENGDKNPPAIFILSPPRSGTTLLRIMLAGH
                     RDLFSASELQLLGFSTLRERAAAYTGRFSPWLDGAIRTAMEVEGLGADDAKALLQTLA
                     SNGLTTKALYRRIQEAVRPRILVDKSPSYALDPSALRRAEVDFDGALYVHLVRHPVPM
                     IESFERHHMDQILYLHEHPYGPRQLAELVWTVSHQNILDFLSEVPRERWFRLQFEQLV
                     TDPAAEMEALCSRFGLSFDPAVIQPYEQVDAKMVDGVYPESAPMGDPGFLAHGRIDPS
                     TAATVLRSGQSLPLGAPTREVAAIFGYDLPVNGSHDRRIGRDALARQRELRRSGSRSN
                     G"
BASE COUNT          754 a         1584 c         1639 g          752 t
ORIGIN      
        1 ggttgccggc agggcggtgg ccctggtcgc gtgtcgcgag gtagtgggtg cgaggggatg
       61 atggcgcagg ggcttcgacg aatggcgcgg gggcatccac ggatggcgtg ggggccatga
      121 gggccggcgc gacggcgtcc gatcggtcgc cggacctcat gagctcgatc tgccgctcca
      181 tgacccgcag ctgttcgcgg atcagccgct cgacggccgt ggtcgatgcc ggctcggttt
      241 cgacgccgtg caattccctg accccaggcc cgctgtcgtc ccggtggccc gccgcgtccg
      301 cgggcgtcgg gctggtcacg accgggcgct gtggccccgg gaatgcctcc ggggcgagct
      361 tgccatcgat gaaccctgcg agcgagtcaa tggaaggagc ctcctcgaag atctgacgga
      421 aggtgatccg caccccgaat tgctttctga actgggcatt ggcttgcgtc agaaacagcg
      481 agtcgaaccc aagatcggca aagctggccg acgggtcgag cgcggacggg tcgaccccgc
      541 tgagctcact gaggatcgac gccaggcgga cgatgatccg gtccttgcgc aattccgtcg
      601 ccggttcggg gccatcggcg acgagattct cggtgcgcgc cacggtggcc tcctgatgga
      661 cgggttcggg gtctggcggt gccgcccgag ttggccgcgg cgccgaggcc gcgccccgcg
      721 accggccgat cgggtcgatc cagtaccgct cgtgatcgaa cggataggta ggaagaacaa
      781 cgcggcgccg ccgtccaccg tggacgccag gccagtcgac gtcgccgcct gccccccaga
      841 cctggccgag ggcccgcagg atccactcct cgtcgctgac ctgctgccgg cgatggcgga
      901 gtgaggagag cacgacccgg tcgggcatcg cctccgggta ctgtcgaacg agcgatgcca
      961 gcgtctggcc agggccgacc tccaggagga tcatgtcggg ctgatcaagc agccggttgg
     1021 cggcgtccac gaagcggacg gtctgctgaa gatgggtcgc ccagtagtcc ggatctgcaa
     1081 gctggtcttc ggccgcccag gtcccggtca cggtgctgac gatcggcacc gtcagcgggc
     1141 cgcgagccgt accccggacg agctcgcgga actgggcgac gatcgggtcc atcatcggcg
     1201 agtgcgcggc aagcgcgatc gggagtcgct ggacctccac gtcggctgcc acgagctgct
     1261 cctcgagccg gctgatcgac gcctccggac ccgaggcgac gcattgcgcg ggcgcattga
     1321 tcgccgcgag cgaggtcgcg gcgtcgagga gcgggatcac ggcctcgggc tcgagcatga
     1381 tcgccaacat gccgccgccg agctgcgcct gcatcagcct tccgcgctcc gcgacgaggc
     1441 gcaaggcatc ctggagcgag aacacccctc cgaggcacgc ggccgcgaac tcgccaaggc
     1501 tgtggcccac catcgcggac ggttgcacgc cccagctcat ccatagccgc gccagggcgt
     1561 actggatagc gaacgtggcg ggctgtgcga gcgcggcatc tccgagctcc tcggcagcgg
     1621 catcgacctt gtccgcttcg gggtagatga cctcgaggag gtcgcggccg aggatcgggg
     1681 cgaggatccg ggcacactcg tcgagtgccg ccttgaacac aggctcgctc tggtacaggc
     1741 ccgcacccat gccagggtgt tgcgctccct ggccggggaa catgaaagcc acccgggcgc
     1801 catcgctcgc ggtcacgcga tcgctgacga cgagcggatc gggctcgcgg agcagcctca
     1861 tagcctcgcg caccgtcgac gccacgaacg accttcgatg cggcatcctc gcccggccga
     1921 gctggctgct gtagccgaca tccgccagct cgaggtccgg gtgtcgctcg agatggtcgg
     1981 ccaacgcacc ccccatcgct tcaagcgcgg cgtgcgactt ggcactgagc gtcacgatct
     2041 ggtgggcctt ccgtggtcct gccggcgcgg gagcccgctc tggcgcctcc tcgagcacca
     2101 ggtgggcgtt ggtgccaccc agcccgaatg cgctcacggc agctcggcgg ggctgttccg
     2161 tgcgcttcca ggctcgcagc tcggtgttca cgaagaaggg gctgccactg aaatcgatct
     2221 gcgggttcgg cctgttgaag ttgaggctgg gtggaatctc ctcgtggtgc agcgacagcg
     2281 cggccttgat gattccggcg acgccggctg ccgcgtcaag gtggccgatg ttgctcttga
     2341 gggagccgat ggcgcagtac tgccggcggc tcgtgtcacg tcggtaggcc tgggtgagcg
     2401 ccgcaacctc gatcggatcc cccagggatg tcccggtccc atgcgcttcg accatcccga
     2461 tcgtgtcggc gtcgacctct gcaacggcga gtgcctcttc gatcaccgcc gtctgcccct
     2521 cgatgctcgg cgccgtgtac ccgatcttgg ccgcgccatc gttgttcacg gccgatccct
     2581 tgatgacggc atagacagtg tcgtcgtccc gaatggcgtc ggacaggcgc ttgagcacga
     2641 cgacggccgc accactggcc aggaccgtcc ctcgcgcatc ggcgtcaaac gcccggcagt
     2701 gtccgtcggg ggacgggatc ccgtcctcct ggtacacgta gccggccgtt gcggggactc
     2761 gaatccggca ggcacctgcc aacgcgaggt cggactcgcc ggagaggaga ctctggacag
     2821 ccaggtggac cgcaacggcg cccgtggaac aggcggtgtt caggctgatg gccgggcctc
     2881 tgagccccag cttgtaggcc actcgagtga tcgcgtactc acgctccgtt gccaggagga
     2941 gcgggtagtc gccggtccga gccaacaggt ccgcgtggtc gacgacattg cggcggaagt
     3001 acgtgttcgg cccgacgccg ccgaatacgc cgatgcgacc gacgtctgcg ccaggttcgt
     3061 agcctgcatg ctcgatggcc gtccaggcac actcgaggaa cacccgatgc tgcggatcgg
     3121 tgagctcggc ctcgcggcgg ctcatcccga agaagccggc atcgaacatg tcgatcccgt
     3181 ccatgacgga gccggcgttc acgaagcccg gctcggatgt gtccgcgccg gcttccgaga
     3241 gctccgcatc cgtgaacgcg cggatcgatt cgacaccgtt tcggagattg gcccagaact
     3301 ggtccacgtc gacggcgccc gggaaccggc cggccatgcc gatgatcgcg atctgctccc
     3361 tgccgtctgc ctcagccatt gctgcgactg cctgaacggc gcagctcgcg ctgccgcgcg
     3421 agcgcatccc ggccgatccg tcgatcgtgt gacccgttga ctggcaggtc gtacccgaag
     3481 atcgccgcga cttccctcgt cggcgcgccg agcgggagcg actgacccga tcggaggacg
     3541 gtcgctgccg tcgaagggtc gatcctgcca tgtgcgagga agccagggtc ccccatcggc
     3601 gccgattccg ggtacacccc atcgaccatc ttggcgtcca cctgctcgta gggctggatg
     3661 accgcgggat cgaacgagag accgaagcgg ctacacagcg cctccatctc ggcagccgga
     3721 tcagtcacga gctgctcgaa ctgcaggcgg aaccaacgtt cgcgcggaac ctcggacagg
     3781 aagtccagga tgttctggtg gctcaccgtc cagacgagct cggcgagctg acgtggtccg
     3841 taagggtgct cgtgcaggta caggatctgg tccatgtggt gtcgctcgaa tgactcgatc
     3901 atcgggaccg ggtgccgaac gaggtggaca tacagggcgc cgtcgaagtc gacttcggcc
     3961 ctgcgcaacg cggacgggtc gagggcgtag gacggcgact tgtcgacgag gatacgtgga
     4021 cgaaccgcct cctggatgcg gcggtagagc gccttggttg tcaaaccatt cgaggccagt
     4081 gtctgcagca acgccttggc gtcgtcggcc ccgaggccct ccacctccat ggccgtccgg
     4141 atcgcaccgt ccagccatgg gctgaacctc cccgtgtatg ccgcggcccg ctcccgcagc
     4201 gtcgagaagc cgagcagctg gagctcgctt gcagagaaga gatcgcggtg gccggcaagc
     4261 atgatccgca ggagcgtggt tccggagcgc ggggggctca gaatgaaaat cgccggtggg
     4321 ttcttgtcgc cgttctccgc gacgatcgcg tccgcaatcg gaactgcggc acgcaatcgc
     4381 gcgacatcgg ccgccgagat cacagccgtg ctcgacgccg gcctgaccag cgcgtggtcg
     4441 cgccccacca ggcgcgcgac tgccgccggg tactgctcct ggaggaacgt ggcgtactcg
     4501 gcgaccgagg gtgcggcaaa gagcgtgacg acgaagatcg actcgcccaa ctccgtctgc
     4561 agctcgttca cgaagcgcgc ggcctgcaac gacgtcccgc caagctcgaa gaaccggtcg
     4621 tggacgccga ccgtatcgat ctcaaggatc gctcgccaca tgtcggcgac gaggcgttcg
     4681 accttggatc gcggcgcggc gtacggcgtt gtcagctcgc gacgggcac
//
LOCUS       DATBSW010000130         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_997690, whole genome shotgun sequence.
ACCESSION   DATBSW010000130 DATBSW010000000
VERSION     DATBSW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997690"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VIF63_03600"
     CDS             complement(<1..1064)
                     /locus_tag="VIF63_03600"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY8238498.1"
                     /translation="MSGPAIGEPDPDRAAPARTGFGDAATGILVLLALGLTLRLIIAY
                     LLPGSGFGVDLSSFQFWANDLADNGLFGFYDRPFFHDYTPGYLYVLWLVGSVANFLGS
                     GVGDLIKVPPIAADLALGYLAWSMTRELGGSERSARIAAALVLFNPVTWFDSVVWGQV
                     DSVGVVFLLLALRELWRDRPERSAILAAVAALIKPQLGILIPIVAIVVIRRAFWPRGG
                     YGLEDEPDAGGTTTSLERRIRGPIRVLTTGLAGLLTTIVVSIPFGLSFPGLIAQIFKT
                     AAGYPYLSVNAFNPWALLVNNVDGRAESIALSRSWACDSTIAPSPGGELRIGDWQVWS
                     WPASTQTCDPGVMIGAFSAAA"
     gene            1094..2560
                     /locus_tag="VIF63_03605"
     CDS             1094..2560
                     /locus_tag="VIF63_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238499.1"
                     /translation="MLGSVAEGAVGSGRPEVLSDWFDRLPALVVGGGLVALALVAYWL
                     PGSDRYYNHFVWQALAFLGGHVAIDWPVDLGGFPRGNDWMQDVYPLRELTGDPSTTGA
                     LLPFPPLPAIVLMPFVAIWGMATDIRAVSVVLGAVAVGLAWWMLGRLPISRRIRLATT
                     IFFAFGTVFWYAAQLGTTWFFAHVVAVTLLLLAVGVALGADPQADDEVEAAPSPGALA
                     AAFGAAVRRPLELIDRRQFLAGLLFGIACTARLTVVFGAPFFMLVGGGGSWVRRSFSA
                     GLGAVIPVGLLLLYNLATTGHVFHPGYEYQYQIEARGYEVLGYNPDWEIEDPRYLPQN
                     LGIMFLTLPAILPEHKVGAFGDTDDPICTEPGASRSLFDRDCPIAIPRTIGMGIFFVS
                     PAFLLAIPALRRGRSRVVAGAFLAVLAITIVNLMHFSQGWVQFGYRFSNDFVVFALPL
                     VALGMARRGRVGWLGAGLVAAAVVINLWGVIWGNILGW"
     gene            2557..>3189
                     /locus_tag="VIF63_03610"
     CDS             2557..>3189
                     /locus_tag="VIF63_03610"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEY8238500.1"
                     /translation="MSSSSTRWLAILAPIAVIAIVIVNAWPGLMPGVGFWDTGEFQTV
                     LPIMGTAHPTGYPTYVLLGFVANLLLTPIGEPAFRINALSLVAVAVAAGATAVLVRRL
                     TGSTIVGAATGLGLATTPVVWLNATRADPHPIHLLFVALLLLALVRWEDARRELRDAA
                     ADVPSEDGADPPADPVAAGRTADRRLVVAAIVFGLAAGNHSLILLFIPPII"
BASE COUNT          419 a         1125 c         1118 g          527 t
ORIGIN      
        1 gcggccgccg aaaacgcccc gatcatcacc ccgggatcgc acgtttgcgt gctcgccggc
       61 cagctccaca cctgccagtc cccgatgcgg agctcgccgc caggcgaggg cgcgatcgtc
      121 gaatcgcacg cccagctccg ggacagcgcg atgctctccg cgcgcccgtc gacgttgttg
      181 acgagcagcg cccacgggtt gaacgcgttg accgagaggt atgggtaacc cgccgccgtc
      241 ttgaagatct gggcgatcag gcccgggaag ctcagcccga aggggatcga gacgacgatc
      301 gtcgtgagca ggccggcgag gcccgtggtg agcacccgta tcgggccgcg gatccggcgc
      361 tccagggatg tggtcgtgcc gcccgcgtcg ggctcgtcct cgaggccgta gccgccgcgc
      421 ggccagaagg cgcgccgaat cacgacgatg gcgacgatcg ggatgaggat cccgagctgg
      481 ggcttgatca gcgccgcgac ggccgcgagg atcgccgatc gttccggccg atcgcgccac
      541 agctcgcgca gcgcgagcag gaggaagacg acgccgaccg agtcgacctg gccccagacc
      601 acgctgtcga accaggtcac cgggttgaac aggacgagcg ccgcggcgat gcgggccgac
      661 cgctcgctgc cgccgagctc ccgcgtcatc gaccaggcga ggtagccgag ggcgaggtcg
      721 gccgcgatcg gcgggacctt gatcaggtcg ccgacgccgc tcccgaggaa gttcgccacg
      781 ctgccgacga gccacaggac gtacaggtag cccggcgtgt agtcgtggaa gaacggccgg
      841 tcgtagaagc cgaagaggcc gttgtcggcc aggtcgttcg cccagaactg gaaggatgag
      901 aggtcgactc cgaagccgga accgggcagg aggtatgcga tgatgagccg gagggtgagc
      961 cccagggcca gcaggacgag gatgccggtt gcggcatctc cgaacccggt ccgcgctggc
     1021 gccgccctgt ccgggtcggg ctcaccgatt gcgggaccac tcaagtcggg atcggtctcc
     1081 agcgagcgat gaggtgcttg ggagcgtagc agagggcgcc gtcggctccg gtcgtcccga
     1141 agtcctgtcc gactggttcg atcggttgcc cgccctcgtc gtcggcggcg gcctcgtcgc
     1201 gctcgccctc gtggcgtact ggctgcccgg ctcggaccgc tactacaacc acttcgtgtg
     1261 gcaggcgctg gccttcctcg gcggccacgt cgcgatcgac tggcccgtgg acctcggtgg
     1321 ctttccccgc ggcaacgact ggatgcagga tgtctacccc cttcgtgagc tgaccggcga
     1381 cccctcgacg acgggcgcgc tcctgccgtt ccctccgctg cccgcgatcg tgctcatgcc
     1441 gttcgtggcg atctggggaa tggccaccga catccgggcc gtctcggtcg tcctgggtgc
     1501 cgttgccgtg ggcctggcgt ggtggatgct cggccggctg ccgatctccc gccggatccg
     1561 gctcgcgacc acgatcttct tcgccttcgg gacggtgttc tggtatgcgg cgcagctcgg
     1621 cacgacgtgg ttcttcgccc atgtcgtggc ggtgacgctg ctcctgcttg cggtcggcgt
     1681 ggccctcggc gcggacccac aggccgacga cgaggtcgag gcggcgccgt cgcccggggc
     1741 actcgccgcg gccttcgggg cagctgtgcg gcggccactc gagctgatcg atcgccggca
     1801 gttcctggca gggctcctgt tcggaatcgc ctgcacggcg cgcctgacgg tcgtgttcgg
     1861 ggcgccgttc ttcatgctcg tcggcggtgg cgggagctgg gtccgccgat cgttctccgc
     1921 cgggctcggt gcggtcatcc cggtggggct gctgctgctc tacaacctgg ccacgaccgg
     1981 acacgtcttc catcccggct acgaatacca gtaccagatc gaggcgcgcg gctacgaggt
     2041 cctcggctac aaccctgact gggagatcga ggatccgcgg tacctgccgc agaacctcgg
     2101 gatcatgttc ctcacgctgc cggcgatcct gcccgagcac aaggtcgggg ccttcggcga
     2161 caccgacgat ccgatctgca ccgagcccgg cgcgagccgc agcctcttcg accgggactg
     2221 cccgatcgcc atcccgcgca cgatcggcat gggcatcttc ttcgtcagcc cggccttcct
     2281 gctggcgatc ccggcgctgc gccgcgggcg gtcgcgcgtg gtcgccggcg cgttcctcgc
     2341 cgtcctcgcc atcacgatcg tgaacctgat gcacttcagc cagggctggg tgcagttcgg
     2401 ctatcggttc agcaacgact tcgtggtctt cgcgctgccg ctcgtcgcgc tgggcatggc
     2461 acggcggggg cgggttggct ggctcggcgc cgggctcgtc gcggctgcgg tcgtgatcaa
     2521 cctgtggggc gtcatctggg gcaacatcct cggatggtga gcagctcctc cacgcgctgg
     2581 ctggcgatcc tcgcgccgat cgccgtcatc gcaatcgtga tcgtcaacgc gtggccgggc
     2641 ctcatgcccg gcgtcggctt ctgggacacc ggcgagttcc agaccgtcct gccgatcatg
     2701 ggcacggccc acccgacggg ctacccgacg tacgtgctcc tcgggttcgt cgccaacctc
     2761 ctgctgaccc cgatcggcga gcccgcgttc cgaatcaatg cgctgtcgct ggtcgcggtg
     2821 gctgtcgcgg ccggcgccac ggcggtcctc gttcgccggc tgacgggctc gacgatcgtt
     2881 ggtgcggcga cggggctcgg cctcgcgacc acgccggtgg tgtggctgaa cgccacgcgc
     2941 gcggacccgc acccgatcca cctgctgttc gtggcgctgc tgctcctcgc gctcgtccgc
     3001 tgggaggacg cgcggcgcga gcttcgtgac gccgcggccg atgttcccag cgaggacggc
     3061 gccgatcctc ccgccgaccc agtcgccgcc gggcgaacgg ccgatcgccg gctcgtggtg
     3121 gccgcgatcg tcttcggtct ggcggcgggc aaccactcgc tgatcctgct cttcatcccc
     3181 ccgatcatc
//
LOCUS       DATBSW010000131         7279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1104132, whole genome shotgun sequence.
ACCESSION   DATBSW010000131 DATBSW010000000
VERSION     DATBSW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104132"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1561)
                     /locus_tag="VIF63_03615"
     CDS             complement(56..1561)
                     /locus_tag="VIF63_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8238501.1"
                     /translation="MKRKLWTMAALAAVFVLIVGACAPAATTAPTTGPVATAGPVATA
                     GPVATAGPVATTAPPVTKNLPAVPTGYTDLDAALTPGADGKMPLAGKKVSIQTQWVGG
                     EGAGFAAAFADFAAATGITIQVDSISSSHEALLKTRIEGGKPPDMAALAQPTGVLAYA
                     AKGQVKDIAAIVGAAGVTKLKAEFPSTIGLTSEGDHIWSIPTKADVKSMIWYPVKAFK
                     AAGYAVPKTWDELVTLADTIAATGAFPFCVSAGGPGAATGWELTDWVEEVLIKTTEPS
                     VTEGWINHTIPFTDPRIKAAFDKVGSLLFKAGYVDGGGSQIVNNDLRTVMDPMFDGDT
                     LTPGCWMQKIPVWYGPDFFPDKRVNGGDSKFVIGDDGDIGIFPFPTIDANLRGAEGSA
                     DSFIVMEDRPEVRAFAEFLATPEGLKGWITTVGVLSPNVKVPADWYANNYKLKVANEI
                     ASGATSINFDASDLMPPSVGQGTFWTQISGWASSNGTTTDQVLADIDASWP"
     gene            complement(1752..2783)
                     /locus_tag="VIF63_03620"
     CDS             complement(1752..2783)
                     /locus_tag="VIF63_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8238502.1"
                     /translation="MSDPRGDIVVGVDIGGTKTAILVCAPDGTVLARSVAPTAVGNRD
                     RAADAISALVEAALHNAGVKRTDVAALGVGVPGRVDRERGHVTLAVNLDWHELPLGPR
                     LEARLGIPTFIDNDVRAAALGLHRRRLFGPVESLALLAIGTGISAGVVLDGVLRHGSL
                     GLAGEIGHAIIDRDGPHCACGNQGCFEAVAAGPAIVTRTLRGWAARRNGDGQPVDPRE
                     TAAGADAVYAAAAAGDAVAREVVESTGRAIAWGIHMLALAYDVERIVIGGGVSHAGEA
                     FMAPIRRELDRYREASPLAAEILLPDLVQLLPAGADAGAWGAVTVARESLGERRGAAV
                     AVRREVGHA"
     gene            complement(2789..4024)
                     /locus_tag="VIF63_03625"
     CDS             complement(2789..4024)
                     /locus_tag="VIF63_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY8238503.1"
                     /translation="MTFPSKATHQQTRAYNAALVLRALYDFGPISRAEVARLSGLTRT
                     TVGDVIGVLMEDGLAREVGRGPSTGGKAPILLELIDGARHVIGLDLGENVFVAALLDL
                     RGQVQRTIERPVVGLDGKQWLEVVHELIDELSRDSRATLLGIGVGTPGIVDAATGTIL
                     WAVNLDWQNLPLGEILRARHGVPVEVANDSRAAALAIELFSGQRRSNLVAIKVGLGVG
                     AGVILGGELFHGDGFGAGEIGHVVVEDDGGECRCGRFGCLETVSSSRAILERATEAAL
                     HDPESPLGRRLVAAADGTLGQAMGRLTLDDVRTAIEAGDEEARRIVVTAGRFLGRAVA
                     GIIGMLDIEHIVLHGSVAQLGEPWLEAVRDEARSRSLGLLTRNVAIELAPPIGDLVVM
                     GASALLLTAELGLASSRAA"
     gene            4744..5745
                     /locus_tag="VIF63_03630"
     CDS             4744..5745
                     /locus_tag="VIF63_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8238504.1"
                     /translation="MTLLLDKLTKRFGPVLALDEISFEVKPGEVFGFLGGNGAGKTTA
                     MRIILDIIRPDSGTVTWKGVPTQEAPRATWGYLPEERGLYPRMEVLEQLEFIGTLFHV
                     PRQESARRAREWLERFAIADYAKRRAEELSKGNQQKIQFIAAVLHEPPVLLMDEPFVG
                     LDPVNVALLKEAFLEMRDRGSTIVFSTHQMEMVEELCETVVLIDRGRVVLGGSVREVR
                     RSTGRQVIRLAVEGDTEMPWLAEVPGVRAVRPAQNYAEVDVEQSVDPETVLRAALDRG
                     KRVTAFLIDDPSIEEIFIERVGRKPTEDQHLGGASAARLAAVVGPAAGAGPRPGGPA"
     gene            5742..7115
                     /locus_tag="VIF63_03635"
     CDS             5742..7115
                     /locus_tag="VIF63_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238505.1"
                     /translation="MKFAAFLATTLGGKLDRNNVVAVARREYLARARTRTFKLTTVLL
                     VVVGVGAALSPLLFRYLDRGTGQTTIEVWVGGSNPAVDVVTGLSAFMNAAGTGASGTP
                     PATPRYRIVAADGLDSARARVLAGDTAGLLVVGRAASGNELTFEYVTTAAAFDRVPQI
                     VSQAAGQLAIQERLSNAGVPPAEQGQLFAPPTYTVTPADPNAKPGASPEEQVGGFAIG
                     FVLAIILFMAIVLYGQWIAYSVVEEKSSRVMEVILGAATPFELLSGKVVGVGALALTQ
                     YAIVFVPATIALLLQSQIAAVVLGGSADAGSTLPAGLSIPLLLAFGVFFVFGFAMYAV
                     LYAGAAALVSRTEDINQIVAPMTLVSTAGYLVAVYSSTGLLDANSTFVVVMSYIPFFS
                     PYLMLSRLGAGLVGPVEVLVAMALLAISVPLALWVAARFYAAGVLMYGQRPSLRLMLR
                     VLRHGAR"
BASE COUNT         1045 a         2487 c         2538 g         1209 t
ORIGIN      
        1 cgcccaccgt tcggtgaggc gccccgcgtg ctatcggggg ttcaaccagc tggtcttatg
       61 gccagctggc gtcgatatcg gccaacacct gatcggtggt ggtgccgtta ctgcttgccc
      121 atccggagat ctgggtccag aaggtgccct gaccgacgga cgggggcatc aggtcggagg
      181 catcgaagtt gatggaggtg gcgcccgagg cgatctcgtt cgcgaccttc agcttgtagt
      241 tgttggcgta ccaatcggcc gggaccttga cgttcggcga gaggacgccg acggtggtga
      301 tccagccctt gagtccttcg ggcgtggcca ggaactccgc gaaggcgcgg acctccgggc
      361 ggtcctccat gacgatgaac gagtcagcgg agccctcggc gccgcgaagg tttgcgtcga
      421 tcgtcgggaa cgggaagatc ccgatgtcgc catcgtcgcc aatgacgaac ttcgagtcgc
      481 cgccgttgac gcgcttgtcc gggaagaagt ccgggccgta ccacacgggg atcttctgca
      541 tccagcaacc cggggtcagg gtgtcaccgt cgaacatcgg gtccatgacg gtccggaggt
      601 cgttgttcac gatctgcgag ccgccgccgt cgacgtagcc ggccttgaac aggagcgagc
      661 caaccttgtc gaatgctgcc ttgatccggg gatcggtgaa cgggatcgtg tggttgatcc
      721 agccctcagt gaccgatggc tcggtcgtct tgatgaggac ttcctcgacc cagtccgtga
      781 gctcccagcc cgtcgccgcg ccaggcccac cggcgctgac acagaacggg aaggcgccgg
      841 tcgcggcgat cgtatcggcg agggtcacga gctcgtccca cgtcttggga accgcgtagc
      901 ccgcggcctt gaaggccttg accgggtacc agatcatcga cttgacgtcg gccttggtcg
      961 ggatgctcca gatgtggtcg ccttccgatg tcaggccgat ggtcgacggg aattcagcct
     1021 tgagcttcgt gacgccggcg gcgccaacga tcgcggcaat gtccttgacc tggcccttgg
     1081 cggcgtacgc gagcacgccg gtcggctgcg caagcgccgc catatccggc ggcttgccgc
     1141 cttcgatgcg cgtcttgagc aaggcctcat ggctcgagct gatgctgtcg acctggatcg
     1201 tgataccggt cgccgccgcg aagtcggcga acgcagcggc gaatccagcg ccctcgccgc
     1261 cgacccactg cgtctggatg gagaccttct tgccggcgag cggcatcttg ccgtcggcgc
     1321 caggggtcaa tgccgcgtcc agatccgtgt agccggtcgg aaccgccgga aggttctttg
     1381 tgaccggcgg tgccgtggtc gcgaccggtc ctgcggttgc gaccggtcct gcggtggcga
     1441 ccggtcctgc ggtcgcgacc ggtcccgtcg tcggcgcagt ggtcgccgcc ggggcgcatg
     1501 ccccgacgat gaggacgaat acggccgcga gagcggccat cgtccacagt ttcctcttca
     1561 tcgatctctc ctccaaattc cgttggtgcc caccagtatg gtggccactg gcaacagggt
     1621 tcatccgatg cgtgttgatc ggtgcaacct ccttcagatg tcccgatctc cggtcgggga
     1681 tggatgtgtg ggtgctgcgt tgctgcgttg gtcagtggcg cgctcgcgtc actgccggga
     1741 gcgcggcccg ctcaggcgtg gcccacctcc ctccgaacgg ccaccgcggc ccctcgacgt
     1801 tcgccgagtg actcacgggc cacggtcacc gctccccatg ctccggcgtc ggcaccggcc
     1861 ggcaggagct ggacgaggtc gggcagcagg atctccgccg ccaggggcga tgcctcgcgg
     1921 taccggtcga gctcacgccg gatcggggcc atgaaggcct caccggcatg ggacacgcca
     1981 ccaccgatga cgatccgttc gacgtcatag gcgagggcga gcatgtggat gccccaggcg
     2041 atggcgcgac cggtcgattc gacgacctcg cgcgcgacgg catcacctgc ggcggcggcg
     2101 gcgtacacgg catcggcgcc ggccgccgtt tcgcggggat cgaccggctg gccatcgccg
     2161 ttgcggcgcg cggcccagcc gcgaagggtt cgagtgacga tggccgggcc ggccgcaacc
     2221 gcttcgaagc agccctggtt gccgcacgcg cagtgcgggc cgtcgcggtc gatgatcgcg
     2281 tggccgatct cgccggccag gccgagggag ccgtgccgga gcacgccatc gaggacgacg
     2341 cccgccgaga tgccggtccc gattgccagc aatgccagcg attcgaccgg cccgaagagg
     2401 cggcgccggt ggaggcccag cgccgccgct cggacgtcgt tgtcgatgaa ggtggggatg
     2461 ccgaggcgcg cttcgaggcg cgggccgagc ggcagctcgt gccagtccag gttgacggcg
     2521 agggtcacgt ggccgcgctc gcgatcgacc cggcccggca cgccgacgcc gagtgccgcg
     2581 acgtcggtgc gcttgacgcc cgcgttgtgg agggccgcct cgacgagcgc ggagatggcg
     2641 tccgcggcgc gatcccggtt gccgacggcg gtcggggcaa cggagcgagc gaggacggtg
     2701 ccatccggcg cgcagacaag gatcgcggtc ttggtgccgc cgatgtcgac gccgacgacg
     2761 atgtcgccgc gggggtcgct catgccgctc atgccgctcg actcgatgcg aggccaagct
     2821 cggccgtcag gaggagcgcc gacgcgccca tcaccacgag gtcgccgatc ggcggcgcga
     2881 gctcgatcgc cacgttcctc gtcagcaagc ccagggaccg gctgcgtgct tcgtcgcgga
     2941 cggcctcgag ccacggctcg ccgagctggg caacgctgcc gtggagcacg atgtgctcga
     3001 tatcgagcat cccgatgatc cccgccacgg cccggccgag gaaccggccg gcggtcacga
     3061 cgatcctgcg ggcttcctcg tcgccggcct cgatcgcggt tcggacgtcg tcgagcgtca
     3121 gtcggcccat cgcctggccg agggtcccgt cggccgccgc caccagacgg cgaccgaggg
     3181 gcgattccgg atcatgcaat gccgcttccg tggctcgttc gaggatcgcg cgcgagctgg
     3241 agacggtctc gaggcagccg aaccggccgc aacggcactc gccgccgtca tcttcgacaa
     3301 cgacatggcc gatctcgccg gcgccgaacc catcaccgtg aaacagctcg ccgcccagga
     3361 tcacgcccgc gcccacgccg aggccaacct tgatcgccac caggttcgat cgccgctggc
     3421 ccgagaagag ctcgatggcg agggccgccg cgcggctgtc gttcgcgact tcgaccggca
     3481 ccccgtgccg ggctcgcagg atctcgccga ggggcaggtt ctgccagtcg aggttgaccg
     3541 cccagaggat cgtgccggtt gcggcgtcga cgatgcccgg tgtcccgacg ccgatcccga
     3601 ggagagtcgc gcgggagtcg cgggacagct cgtcgatgag ctcgtggacg acctcgagcc
     3661 actgcttgcc gtcgaggccg acgaccggcc gctcgatcgt tcgctggacc tgcccgcgca
     3721 ggtcgagcag cgcggcgacg aagacgttct cgccgaggtc gaggccgatg acgtgccgcg
     3781 cgccatcgat gagctcgagc aggatcggcg ccttgccgcc ggtcgacggc ccccgcccga
     3841 cctcgcgcgc gagcccgtcc tccatgagca cgccgatgac gtcaccgacg gtcgtgcgcg
     3901 tgagcccgct gagcctggcc acctcggcgc ggctgatcgg cccgaagtcg tacagggcgc
     3961 gcagcacgag ggcggcgtta taggcccgcg tctgctgatg ggtcgccttg ctcgggaacg
     4021 tcaacgtgtg tacgccgctc ctgtccctgg ctgccctcgc cgggtcccga ttcgctggct
     4081 tcgttgccga ttcgcctgtg tcgttgccgg cttatctcgc ttccatgccg gctgagtctc
     4141 gcttccttgc cggctcgtct cgcttcgatg ccggccgctc gcttggtcgc gcggctcgct
     4201 ccgtcctcgc ggagcggtcg ctgctccttg gctctccgcg cctctcccag gcgggacttt
     4261 gcccagcgac ttgacaaact ctacccacct ttgtcaagtg tctggacgaa gcagccggtg
     4321 agagggatcg agggcatctg acgcagccgc agaagctggg tcggcatgca cgccgggacg
     4381 gggtcgcgct cgcctgcgtg cgggtccaag aaagcggtac tttccaacag cggtgcctcg
     4441 aggtcgcgcc atatggaccg cacgggcacg ttaggtctcg tgaaaggcgt ttgcggcgtc
     4501 tccgcaatcg ccagcggcgg tggactcggc gcgagttgaa cgtgaatcga ccacaccgaa
     4561 aggcttggcc gccggccaga cccgcgttga gaggccagcg acggacctaa cgtgcccaga
     4621 acgcgcaaat ggcgcaaacg tggtgccgca acggaggcag atgcctcgga tggagtcgcc
     4681 gcagctgtga cttgcgcctc ggacgcaggt gccccagcgg caagaggcag tagcctctac
     4741 ggcgtgacgc tccttctcga caagctgacc aagcggttcg gcccagtgct cgcgctcgac
     4801 gagatctcgt tcgaggtcaa gccgggcgag gtgttcgggt tcctcggtgg caacggcgcc
     4861 ggcaagacga ccgcgatgcg gatcatcctc gacatcattc gacccgactc cggaacggtg
     4921 acctggaagg gagtgccgac gcaggaggcg ccccgcgcga cgtggggcta tctgccggag
     4981 gagcggggcc tgtatccccg catggaggtc ctcgagcagc tcgagttcat cgggaccctg
     5041 ttccacgttc cgcgccagga aagtgcgagg cgcgcgcggg agtggctcga gcgcttcgcg
     5101 atcgccgact acgcgaagcg gcgcgccgag gagctgtcga agggcaacca gcagaagatc
     5161 cagttcatcg ccgcggtcct ccacgagccg ccggtgctgc tgatggacga gccattcgtc
     5221 gggctcgacc cggtcaacgt cgcgctgctc aaggaagcgt tcctcgagat gcgcgaccgc
     5281 ggctcgacga tcgtcttctc gacccaccag atggagatgg tcgaggagct gtgcgagacg
     5341 gtcgtgctga tcgatcgcgg gcgggtcgtg ctgggcggct ccgtgcgcga ggttcgccgc
     5401 tccaccggcc ggcaggtcat ccgactggcg gtcgagggcg acaccgagat gccctggctc
     5461 gccgaggtgc ccggcgtccg cgcggtgcgg cctgcgcaga actacgcgga ggtcgacgtc
     5521 gagcagtcgg tcgaccccga gacggtcctg cgcgcggcgc tcgatcgggg caaacgcgtg
     5581 acggcgttcc tgatcgacga cccatcgatc gaggagatct tcatcgagcg cgtcgggcgc
     5641 aagccgaccg aggaccagca cctcggcggc gcgtcggcgg cgcggctggc ggcagtggtg
     5701 gggcccgctg caggtgccgg tccccgacct ggcggcccgg catgaagttc gccgcgttcc
     5761 tcgccacgac gctcggcggc aagctcgatc ggaacaacgt ggtcgccgtc gcccggcggg
     5821 agtacctggc gcgcgcgcgc acccgcacgt tcaagctcac gacggtgcta ctggtcgtcg
     5881 tcggtgtcgg cgccgcgctg tcgccgctgc tcttccggta cctcgaccgg ggcacaggcc
     5941 agacgacgat cgaggtatgg gtcggcggtt cgaacccggc ggtcgatgtc gtcacgggcc
     6001 tctcggcctt catgaacgcc gcgggcacgg gagcgagcgg cactcccccc gccaccccga
     6061 ggtatcgaat cgtcgcggcc gacggtctcg attcggcgcg tgctcgagtg cttgccggcg
     6121 ataccgcggg cctgctcgtg gtcggcaggg cggcgagcgg caacgagctc acgttcgagt
     6181 acgtgacaac ggctgccgcc ttcgatcgag tgccccagat cgtgagccag gcggcgggcc
     6241 agctcgcgat ccaggagcga ctctcgaatg ccggcgtgcc acccgccgag caggggcagc
     6301 tgttcgcgcc gccgacctac acggtcacgc ctgccgaccc caacgcgaag ccgggcgcaa
     6361 gtccggagga gcaggtcggc ggattcgcga tcgggttcgt gctcgcgatc atcctgttca
     6421 tggccatcgt cctgtacggc cagtggatcg cctacagcgt cgtcgaggag aagagcagcc
     6481 gcgtcatgga ggtgatcctc ggcgcggcga cgccgttcga gctgctgtcg ggcaaggtgg
     6541 tcggggtcgg ggcgctcgcg ctcacccagt acgcgatcgt gttcgtgccg gcaaccatcg
     6601 ccctgctgct ccagtcacag atcgccgcgg tcgtcctcgg cggctcggcc gatgccggga
     6661 gcaccctgcc ggccggcctg tcgatcccgc tgctgctggc gttcggcgtg ttcttcgtct
     6721 tcggcttcgc catgtacgcg gtgctctacg cgggcgccgc agccctggtc agtcgaacgg
     6781 aggacatcaa ccagatcgtg gcgccgatga ccctggtctc gacagccggc tatctcgtcg
     6841 ccgtctactc gtcgacgggc ctcctcgacg ccaactcgac cttcgtcgtc gtgatgtcgt
     6901 atatcccgtt cttcagcccg tacctgatgc tctcccgcct cggtgcgggc ctcgtcggtc
     6961 cggtcgaggt cttggtcgcg atggccctgc ttgcgatcag cgtcccgctc gccctctggg
     7021 tcgcggcccg cttctacgca gcgggcgtcc tgatgtacgg ccaacgcccc tcgctccggc
     7081 tcatgctccg cgtcctccgc cacggagccc gctgaccctc gaccaggtcg ggcgcgtcag
     7141 gccttcgtcg cggcccccga cgatgagaac gccgcggccg acgttggctt gttgtcgctc
     7201 ttcggcgagg tgtcgccgct ccagtagcgc ttctggtcct tccagtactg gttgtccggg
     7261 ttcttcttca tctgcttgt
//
LOCUS       DATBSW010000132         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_786496, whole genome shotgun sequence.
ACCESSION   DATBSW010000132 DATBSW010000000
VERSION     DATBSW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786496"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIF63_03640"
     CDS             <1..217
                     /locus_tag="VIF63_03640"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238506.1"
                     /translation="PALVAAARDVGALGACLSGSGSTIVAFVDASDETAADRVGAAFR
                     STAARLNQPGRLIALKPANAGARVTSP"
     gene            506..934
                     /locus_tag="VIF63_03645"
     CDS             506..934
                     /locus_tag="VIF63_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238507.1"
                     /translation="MSERELLATFDALAELPGFQVGGLTPAGDEVLVYWHGELGPEAK
                     AAVDEASRHGVVVNVISVPYSYDELREIAGPLVEALAAKGIETEGYQIGDPFDVIAVW
                     GTALDGSAEARRAAEEIAADILPPDLRFAIIASPGPAVLL"
     gene            964..1470
                     /locus_tag="VIF63_03650"
     CDS             964..1470
                     /locus_tag="VIF63_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8238508.1"
                     /translation="MTLGTTNKQTRLVLLCGLPASGKTTLAHELADAYGAVRLDPDEW
                     EVALGVDPFDERFQTKLEAAFWELAQRLLALGTSVVLEWGFWARSERDEKREAARRLG
                     VAVELRFLDAPQEELVRRVVARHAAGGIAITESHMERYRRLFEPPTDQELRLYDPAAE
                     HGAAGGSR"
     gene            1483..1953
                     /locus_tag="VIF63_03655"
     CDS             1483..1953
                     /locus_tag="VIF63_03655"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8238509.1"
                     /translation="MNDGKAITTRTTFRLAISVGILIEAPATIIWPLLTDLHAQATWN
                     STLASIDGKVALGERVSFRVPEAPNQTFSPTVVGYDEGNSMVWRLSRWPLLVSDRTYR
                     LKPGPEGSTEFTLDEVFRGLLLPLIVKTFPDFGRMFERTAADLKAEAERQASGA"
     gene            complement(1954..2865)
                     /locus_tag="VIF63_03660"
     CDS             complement(1954..2865)
                     /locus_tag="VIF63_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8238510.1"
                     /translation="MRPIEIGLVLPMGNSFVDGSTARWVEIRDLAVRAEEFGFDTVWV
                     ADELLWRSAEGQPQGWWEAVAMTAAVAAATSRVKVGTWVMSALHRNPGITAKAVETID
                     EISAGRFVFGLGSGHAGRQGHAFGLPEDHVYSRFEEALAIIIPLLREGRVDFEGTYHA
                     ARDLEQRPRGPRPGRIPIMIGAGSPKMLRQAALHADIWSWYVQERSDLVEFGPRLAAL
                     EAACAEVGRDPATIGKSAGIVVEAPGFSGAEAQFGPPVRGTAEEIADACRAFGAAGYP
                     NLELIVWPPTMAALDALAPVIELLDAD"
BASE COUNT          454 a         1006 c          994 g          480 t
ORIGIN      
        1 gcccgccctc gtcgccgccg cgcgcgacgt cggcgccctc ggcgcctgcc tctcgggcag
       61 tggctccacg atcgtcgcct tcgtcgacgc ctcggacgaa accgccgccg accgcgtcgg
      121 cgccgccttc cgctcgaccg ccgcccgcct caaccaaccc ggccgcctga tcgcgcttaa
      181 gccggccaat gccggcgctc gcgtgacatc gccctgacgg atgtcggccg accctcactc
      241 cgttcgttgt tgacgtttgg cccgcgacag ccatgctccc tgaaatgagt gtcacgcggc
      301 gaacgggtca gcagcattac ccggcgcggt agctcgacgt ctgcccgttg tgctgaagcg
      361 gtcgttcggc gtcctcgtca ttgcagcggt gctcgttgcc tgctcgtccg cgggcgcgtc
      421 ggtcgcgccg tcggcgtcga ccgcgtcgga gcatgatcga tccccggcgc ccaccagcgc
      481 gtcgcccgcc ccgtcggcgt cgaccgtgtc cgagcgtgaa cttcttgcga ctttcgatgc
      541 gttggctgag cttccagggt tccaggttgg tgggctgacg cccgcgggcg acgaggtcct
      601 ggtctactgg cacggcgagt tggggcctga agccaaagcc gccgtcgacg aggccagtcg
      661 acacggtgtt gtggtcaacg tcatctcggt tccctactcg tatgacgagt tacgcgagat
      721 cgctggccca cttgtcgagg cgcttgcggc gaagggcatc gagaccgagg gctaccagat
      781 cggagatccg ttcgacgtga tcgcggtgtg gggaactgcc ctcgacggat ccgccgaggc
      841 acggcgcgcc gcagaggaga tcgccgccga cattctccca cccgacctca ggttcgcgat
      901 catcgcgtcg cctggtccgg cagttcttct gtagaactcg caccggcagc tgtgagcggc
      961 gcgatgacac tgggaacgac gaacaagcag acgaggctcg tgttgttgtg tggccttcct
     1021 gcgtcgggga agacgacgct cgcgcacgag ctggcggacg cctatggcgc ggtgcggctc
     1081 gaccccgacg agtgggaggt ggcgttgggc gttgacccat tcgacgagcg gttccaaacc
     1141 aagctcgagg cggccttctg ggaacttgcc cagcgtcttc tcgcgctcgg aacctcggtt
     1201 gtccttgagt ggggcttctg ggctcggtcg gaacgggacg agaagcgcga ggccgcgcga
     1261 aggctcggcg tcgcggtcga actcaggttc ctcgatgcgc cccaggagga gctggtgcgc
     1321 cgagtcgtgg ctcgtcacgc tgccggcggc atcgcgatca cggagagcca catggagcgc
     1381 tatcgacgct tgttcgagcc gccgaccgat caggagttgc gcctctacga cccggcggcg
     1441 gagcatggcg ccgccggtgg atcccggtag gctgaggttc cgatgaacga cggcaaggcc
     1501 atcacgacgc gaacgacatt tcgactggcg atctcggttg ggatcctcat cgaagcgccg
     1561 gcgacgatca tctggccgct gctgaccgac ctccacgcac aggcaacgtg gaactcgacg
     1621 ctggcgagca tcgacggaaa ggtggccctc ggggaacgcg tctcgttccg ggtccccgag
     1681 gcgccgaacc agacgttcag ccccacggtc gtcggatacg acgagggcaa ttcgatggtc
     1741 tggcggctca gccgctggcc gctgctcgtg agcgatcgga cctaccgact caagccaggg
     1801 cctgaaggat cgaccgagtt cacgctcgac gaggtctttc ggggcctgtt gctcccgttg
     1861 atcgtcaaga cctttcccga tttcggtcgc atgttcgagc ggacggccgc cgacctgaag
     1921 gctgaagccg agcggcaggc gagcggggcc tagtcagtcg gcgtcgagca gctcgatgac
     1981 cggggccaat gcgtccagcg ccgccatggt cggcggccag acgatgagct cgagattcgg
     2041 gtagcccgcg gcgccgaagg cccggcacgc gtcggcgatc tcctcggccg taccgcggac
     2101 agggggccca aactgggcct ccgcgccact gaaccctggg gcctcgacga cgatcccggc
     2161 cgacttgccg atcgtggctg gatcgcgacc aacctcggcg caggccgcct cgagcgccgc
     2221 aagccgcggt ccgaactcca cgaggtcact gcgctcctgg acgtaccagc tccagatgtc
     2281 ggcgtggagc gcggcctggc ggagcatctt gggactgccg gcgccgatca tgatcgggat
     2341 ccggcccggc cgtgggccgc gtggtcgctg ctcgaggtca cgcgccgcgt ggtaggtgcc
     2401 ctcgaagtcc acgcgacctt cccgcagcag cgggatgatg atcgcgaggg cctcctcgaa
     2461 gcggctgtag acgtgatcct ccggcagtcc aaatgcatgc ccctgacgac cggcgtgccc
     2521 tgacccgagg ccgaacacga atcggccggc gctgatctcg tcgatggtct cgaccgcctt
     2581 ggcggtgatc ccgggattcc ggtgcagcgc cgacatgacc cacgtcccga ccttgacccg
     2641 cgaggtcgcc gctgcgacgg cggcggtcat cgcaacagct tcccaccagc cctgcggctg
     2701 accctcggcc gagcgccata ggagctcgtc ggcaacccag accgtgtcga acccgaactc
     2761 ctcggctcgc accgcgagat cgcgaatctc gacccagcgg gccgtcgagc cgtcgacgaa
     2821 ggagttaccc atcgggagca cgaggccgat ctcgatcgga cgcatggaac ctccgcaggc
     2881 gcaggggccg gagtctaggc cgccgcgagt accgtcatgg acgccatccg gttc
//
LOCUS       DATBSW010000133         9129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_751236, whole genome shotgun sequence.
ACCESSION   DATBSW010000133 DATBSW010000000
VERSION     DATBSW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751236"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VIF63_03665"
     CDS             complement(<1..379)
                     /locus_tag="VIF63_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238511.1"
                     /translation="MTARTITRSLALLAGAAVLVAACGGNAATTGPGGTTAPVVTPAP
                     ATPAPVTQGPAGTVNPDLTFDTSSFHADVDLEALFPKEVGGDPLTVLSMSGADMMGEG
                     GSPELEAMLTSLHKTPADLTAAFG"
     gene            complement(463..1656)
                     /locus_tag="VIF63_03670"
     CDS             complement(463..1656)
                     /locus_tag="VIF63_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8238512.1"
                     /translation="MDRARLAVIGAGTMGAGIAQVALEGGWRVCLHDPLPGATDRAMD
                     RIRQGLIRRAEKAGDRDPAAAAATRLLDLAVVRDVAGAADGAQLVIEAAIEDLDAKRA
                     IFAALDDAAAPETILATNTSALSVTSIAEATRRPERVVGLHFFNPAPVLPLVEVVAGR
                     RTAAWAAETGANIVEGWGKTAIRVADSPGFIVNRVNRPFTLEPLRLLRAGVATIETVD
                     AALTGAGFPMGPFALMDLIGIDVNLAAARGLFEASGRPPRFRPSPIQEELVAAGRLGR
                     KSGEGFYRYAEGRANGPAARFAGEIRPARGAPMALDAPTIAERVILAIVNEAYRALGD
                     KVASEADIDLAMRLGANHPTGPFEWARRTGVHEAVVMLDALSDEDADTFRPALPLLRE
                     ARASM"
     gene            complement(1683..2405)
                     /locus_tag="VIF63_03675"
     CDS             complement(1683..2405)
                     /locus_tag="VIF63_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238513.1"
                     /translation="MRSTMAAGGVAAIGFGLAGLVATILQISQQTLGFEDTDDPAVNL
                     TFLADHLDNYLQQGLAFMVMALALTIVVFATWDILAGQRGSLGLRTVSTIGLAAALCL
                     FLFGVFRYSVRPLLYIDSLDTAWGEAAYLVQQVAGVHGVGQAGILTMCGWAVGVAILG
                     ARSGALPRWLAILAVLPAFRILSGLGPLLGPNGLPDEVWILAILSIPGSFVWFIVLGA
                     VFVRRGLGAARPTAPAPTIAAA"
     regulatory      2522..2635
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2763..5669
                     /gene="gcvP"
                     /locus_tag="VIF63_03680"
     CDS             2763..5669
                     /gene="gcvP"
                     /locus_tag="VIF63_03680"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY8238514.1"
                     /translation="MSETKQTVGFVDRHVGPDEREVAAMLASIGYESLDALIRATVPS
                     AILRRDVLGLPAARSEAEALADLRDMAAANTPLRPMIGMGYHDTITPPVILRNVLENP
                     AWYTAYTPYQPEIAQGRLEALINFQTAICDLTGMEIANASMLDEGTAAAEAVTLCHAV
                     STVGDGNLLLVDSDTHPQTIGILRTRAEPLDVQVVVAGVPELLARAGAPKEGERPFAL
                     LLSNPGSSGAVRSIRGDIEAAHAAGLLVVVATDPLAALLIESPGDQGADVVVGNSQRF
                     GVPMGNGGPHAGFMATRDGFKRSLPGRLVGVSIDADGAPAYRLTLQTREQHIRREKAT
                     SNICTAQVLLAVVASMYAAWHGPDGLRAIAERAHVRAATIATAVAEMAGLELRHDQYV
                     DTIAIETNTTGRADALLAAAAAAGYELRRLDATGIAIACDETTTEADVAAVIAAIGAT
                     DPKAAAVTKTAKASLPAAARRTDAILSHPVFSLYRSETEMLRYLFHLAEKDVTLTRSM
                     IPLGSCTMKLNATTEMMGIGLPGFSRVHPFSDPTRLPGYARLYDDLESWLGEITGFAA
                     VSLQPNAGSQGEYAGLLAIRRWHLSRGEGNRKVCLIPASAHGTNPASAVMAGYRVVVV
                     ATDAHGNVDLGDLRAKIAANAADLGALMITYPSTHGVFEAGVVEICRLVHEAGGQVYM
                     DGANMNAQVGLSRPGDIGADVCHLNLHKTFAIPHGGGGPGMGPIGVAAHLRSFLPGIG
                     LDEVGRVSSAPFGSASILPISWAYIAMLGAEGVRRSTQVAILNANYLAKRLGDAFPVL
                     YVGDEGTVAHECILDCRPLTAESGVTVDDIAKRLMDFGFHAPTMSFPVVGTLMIEPTE
                     SESKAELDRFADALLQIRAEIADVEAGRVPVESSALRNAPHTATDLLRDEWARPYTRE
                     QGAFPAPWVRHRKYWPPVRRIDNVYGDRNVVCECLPVEAYAEASGETQLLMVAG"
     gene            5695..6276
                     /locus_tag="VIF63_03685"
     CDS             5695..6276
                     /locus_tag="VIF63_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY8238515.1"
                     /translation="MHDAPLVHAEDRATWRAWLAANHATETGVWLVSWRRGHGPSVPY
                     EEAVEEALCFGWIDSQGGNLDATRSRQYYAPRNPTSGWAATNKARVERLIADGLMAPA
                     GLAAIERAKVNGAWTLLDDVERGIVPDDLAAALAEHPPAASHFESFPKSAKRAMLEWV
                     RQAKRPETRAKRVLDIAERAAENVRAYPPPERR"
     gene            complement(6280..6786)
                     /gene="paaD"
                     /locus_tag="VIF63_03690"
     CDS             complement(6280..6786)
                     /gene="paaD"
                     /locus_tag="VIF63_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY8238516.1"
                     /translation="MIAAPRLIGRSEVLAALDEVADPEIPNVSVVELGMIGEIAFDDD
                     LIAVEVLPTFVGCPALEVIRDTVAARLAEFGRPVRVDVSFATPWSTERISPAGREKLR
                     QSGFAPPPTAPLGRALPMLDLAAAPVACPYCGSLQTTLESAFGPTQCRSIRHCAACGQ
                     PFEAFKPV"
     gene            complement(6786..7568)
                     /gene="paaC"
                     /locus_tag="VIF63_03695"
     CDS             complement(6786..7568)
                     /gene="paaC"
                     /locus_tag="VIF63_03695"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY8238517.1"
                     /translation="MTATEAPIDARTRTALAELLLTMADDEFVGGFTDSEWTGIAPLL
                     EEDIAISSISQDELGHARALYQLLAGVLADGRDEDALAYDREPDEYRHARLLDHARGD
                     WADTIARRFLYELADEARLASIGPGFKPLADLVEKLRREERYHRMHALAWIDRLARGG
                     EEPRRRLEAALTALAADASTVFTPLEGEAALLEARILSRSVADAEAAWRADLSTILVP
                     LGLALPPRMPPAADGRTGHSGSFRWLWGEFTSVRRSEDGATW"
     gene            complement(7565..8161)
                     /locus_tag="VIF63_03700"
     CDS             complement(7565..8161)
                     /locus_tag="VIF63_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238518.1"
                     /translation="MRTIAASCVAAFLVAGCGIGAIFAPAPDDLSTWQAVPLAPDAGL
                     SAKAAQSPACRPALEQDVALEVILQDRRTSATAAFLVQGPTMVGSCYISPTGGGGGSQ
                     PAGQLELLDQPISIDEEGSGGADVGTLSYLGGRLGAEARQVVIEVPGGRKVTASVGNG
                     HWLAWWPGGARAERVVASDEAGAQLFALTLGQDGWVAQ"
     gene            complement(8158..8535)
                     /locus_tag="VIF63_03705"
     CDS             complement(8158..8535)
                     /locus_tag="VIF63_03705"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238519.1"
                     /translation="MAARAAPKRVELEAYEVFQQAKDGDPMRHGGNVMAPDAELAMHY
                     ARELFGRRNESSRLWIVRRGDIAVLEDPDLLDPPLDRSFKKPGGYVMRDKLAAARERA
                     GTSKPAARAETHDRTGGRRAGQP"
     gene            complement(8535..>9129)
                     /locus_tag="VIF63_03710"
     CDS             complement(8535..>9129)
                     /locus_tag="VIF63_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEY8238520.1"
                     /translation="VFHYPTKSWGDIGIIAWLVDAAAIVAQQALRDSSYAPYARTMQK
                     ICWEESVHIMHGRDVVVTLMTGTDSQRALVQEALDRWWGPLMQMHGPRGDRAKDRDLH
                     WRIKAKTSEELRQEFLTIYVPRLLELGLTIPDPELRLDEATKTWSYTEPSWEELRSVV
                     TGHGPKSQERLEFRRLNREDTRWVRETVFGLASMAAA"
BASE COUNT         1387 a         3270 c         3224 g         1248 t
ORIGIN      
        1 cgccgaaagc agccgtgagg tcggctggag tcttgtggag cgaggtgagc atggcctcga
       61 gctcgggcga accgccttcg cccatcatgt ccgcgccgct catgctgagg acggtcagcg
      121 ggtcgccacc gacctccttc gggaacaacg cttcgaggtc gacgtcggcg tggaacgacg
      181 acgtatcgaa cgtcaggtcg gggttgacgg tcccagccgg gccctgggtg accggcgccg
      241 gcgtcgccgg ggccggtgtg acgaccggcg cggtggtgcc gcccggaccc gtcgttgcag
      301 cgttgccgcc gcatgcggcg acgagcacgg ccgcgccggc aagcagggcc agcgatcggg
      361 tgatggttcg agcggtcatg tctggtgcgc cccctcccgt ttgggatcga tgccgaggtt
      421 tcgcggctcg tatctccgcg tggcgcggat cgtactccac gtctacatcg atgcccttgc
      481 ctcccgtagg agcggcagcg cggggcgaaa cgtatcggca tcctcgtcgc tcagtgcgtc
      541 gagcatcacc accgcttcgt ggacacccgt gcgccgggcc cattcgaacg gtccggtcgg
      601 gtggttggcg ccgaggcgca tcgccaggtc gatgtcggcc tcggaggcga ccttgtcgcc
      661 gagggcgcga tacgcctcgt tgacgatcgc caggatcacg cgctcggcga tcgtgggggc
      721 atcgagggcc attggcgccc cgcgcgccgg tcgaatctcg cccgcgaacc gcgcggccgg
      781 cccgttcgca cgaccctcgg cgtaccgata gaagccctcg ccgctcttgc gcccgagccg
      841 gcccgcggcc acgagctctt cctggatcgg cgacggtcgg aagcgcggcg gccggccgga
      901 ggcttcgaac aggccgcgag cggcggcgag gttcacgtcg atcccgatga ggtccatcag
      961 cgcgaacggt cccatcggaa agccagcgcc agtcaatgcg gcgtccaccg tctcgatcgt
     1021 ggccacgccg gcccgaagga gccgcaacgg ttccagggtg aacgggcgat tgacccgatt
     1081 cacgatgaag ccgggcgaat cggccacccg gatcgcggtc ttgccccagc cctcgacgat
     1141 gttcgccccg gtctcggccg cccaggcggc tgtgcgtcgt cccgcgacga cctcgacgag
     1201 tggcaggacc ggcgccgggt tgaagaagtg cagcccgacg acgcgttccg gcctgcgcgt
     1261 cgcctcggcg atcgaggtca ccgagagcgc gctggtgttc gtcgcgagga tggtctcggg
     1321 cgccgcggcg tcatcgaggg cggcgaagat ggcgcgcttc gcgtcgaggt cctcgatcgc
     1381 cgcctcgatg acgagctggg cgccgtcggc ggcacccgcc acgtcccgga caaccgcgag
     1441 atccaacagt cgcgtcgcgg cagcagccgc ggggtcgcgg tccccggctt tctccgctcg
     1501 ccggatcaag ccctgccgga tccgatccat ggcccgatcg gtcgcgcccg gcagcggatc
     1561 gtggagacag acccgccaac cgccttcgag ggcgacctgg gcgatcccgg cacccatcgt
     1621 cccggcaccg atcacggcga gcctggcgcg atccacccgc agacggtacc ccgtccgcga
     1681 cgtcaggccg cggcgatcgt gggcgcaggt gccgtgggtc gtgccgcccc gagccctcgg
     1741 cggacgaaca ccgcaccaag cacgatgaac cagacgaagc tccccgggat cgagaggatg
     1801 gccaggatcc agacctcgtc cggaagccca ttcggaccga ggagcgggcc caggcccgac
     1861 aggatccgga aggctggcag gacggcgagg atcgcgagcc agcgcggcaa cgcgcccgat
     1921 ctggcgccca ggatcgcgac gccgacagcc cagccgcaca tcgtgaggat ccccgcctgc
     1981 ccgacgccgt ggacgccggc gacctgctgg acgaggtagg cggcctcacc ccacgccgtg
     2041 tccagactgt cgatgtagag gaggggccgg acgctgtagc ggaacacgcc gaacaggaag
     2101 aggcagaggg ccgccgcgag cccgatggtc gaaacggttc gcagccccag gctgccgcgc
     2161 tggccggcga ggatgtccca cgttgcgaac acgacgatcg tcagcgcaag cgccatgacc
     2221 atgaacgcca ggccctgctg aaggtagttg tcgagatggt cggcgagaaa ggtgaggttc
     2281 accgccgggt cgtcggtgtc ctcgaagccg agcgtctgct gggaaatctg caggatcgtc
     2341 gcgacgaggc ccgccagccc gaacccgatc gccgcgactc cgccggccgc catggtggat
     2401 cgcatgcaac ctcctgctct gcagctatgc cgcgcaccgc tatatcggct cgcagcgtag
     2461 tggatggagg tccccggttg ccgtgcgaac gaaggcgtag gatgacggtg aatttcccca
     2521 tgcgaactgc gcgggagaga tcgccggctg cgggccatcg gcggcccatg gatccggcgg
     2581 cgccgacgga gcaacggtcc ggaacctctc aggcaccagg gaccgcgcag ggatggggac
     2641 gctggagagc gttgaggacg gggcgacccg ccagcgcgcc gacggaggaa ctcgcggggc
     2701 ggacgctccg cggggacgct ctcaggcacc aggacagcgg ggcaccggga ggtgccgcgc
     2761 tgatgtcgga gaccaagcag acagtcgggt tcgtcgatcg gcacgtgggg cccgacgagc
     2821 gcgaggtcgc ggcgatgctc gcgagcatcg gctacgagtc cctggatgcg ctgatccgcg
     2881 cgaccgtccc gtccgcgatc ctgcgtaggg acgtcctcgg gctgcccgcg gcgcgaagcg
     2941 aggccgaggc gctcgccgac ctccgggaca tggccgctgc gaacaccccg ctccgcccga
     3001 tgatcgggat gggctaccac gacaccatca cgccgcccgt gatcctgcgc aacgtgctcg
     3061 agaaccccgc ctggtacacc gcctacacgc cataccagcc cgagatcgcg cagggccggc
     3121 tcgaggcatt gatcaatttc cagacggcca tctgcgatct caccggcatg gagatcgcca
     3181 acgcgtcgat gctcgacgag ggcacggcgg cggccgaggc ggtcacgctc tgtcacgccg
     3241 tcagcacggt cggcgacggc aacctcctgc tcgtcgactc ggacacgcac ccgcagacga
     3301 tcgggatcct gcgcacgcgc gccgagccgc tcgacgtcca ggtcgtcgtc gcgggcgttc
     3361 ccgagctgct cgcccgcgcc ggcgcgccga aggagggcga gcgcccgttt gcgctgctgc
     3421 tgtccaaccc gggctcgtcg ggcgcggtcc gctcgatccg gggcgacatc gaggcagccc
     3481 acgcggcggg cctgctcgtc gtcgtcgcga cggatccgct cgcggccctc ctcatcgaga
     3541 gccccggcga ccagggcgcg gacgtcgtcg tcggcaacag ccagcgtttc ggcgtgccca
     3601 tgggcaacgg cggcccgcac gccgggttca tggcgacgcg ggacgggttc aagcgctcgc
     3661 tgccggggcg actcgtgggc gtgagcatcg acgcggacgg cgcgcccgcc taccggctca
     3721 cgctccagac ccgcgagcag cacatccgcc gtgagaaggc gacgtcgaac atctgcaccg
     3781 cccaggtgct cctggcggtg gtcgcgagca tgtatgcggc ctggcacggg cccgacggcc
     3841 tgcgcgccat cgccgagcgc gcccatgtgc gcgccgcgac catcgcgacc gccgtcgccg
     3901 agatggccgg cttggagctc cggcacgacc agtacgtcga caccatcgca atcgagacga
     3961 acaccaccgg ccgcgccgat gccctgctcg ccgccgccgc agccgccggc tacgagcttc
     4021 ggcgcctcga cgccaccggc atcgcgatcg cctgtgacga gacgaccacc gaggcggacg
     4081 tcgccgcggt catcgctgcc atcggcgcca cagacccgaa ggcggcggcc gtcacgaaga
     4141 ccgcgaaagc ctcgctcccg gcggcggccc gtcggaccga cgcgatcctc tcccacccgg
     4201 tgttctcgct gtaccgctcc gagacggaga tgctccggta cctcttccac ctcgccgaga
     4261 aggacgtgac cctcacccga tcgatgatcc cgctcggcag ctgcacgatg aagctcaacg
     4321 cgacgacgga gatgatgggc atcggcctgc ccggcttctc gcgggtccat cccttcagcg
     4381 atcccactcg cctgcccggc tatgcccggc tgtacgacga cctcgagtcg tggctcggcg
     4441 agatcacggg cttcgcggcg gtgagcctcc agccgaatgc cggatcgcag ggcgaatacg
     4501 cgggcctgct cgcgatccgc cggtggcacc tgtcccgcgg cgagggcaat cggaaggtct
     4561 gcctcatccc ggcgtccgcg catgggacca accccgcgag cgcggtcatg gccggctacc
     4621 gcgtggtcgt cgtggcaacc gacgcgcatg ggaacgtcga cctcggcgac cttcgggcga
     4681 agatcgcggc caacgccgcg gacctgggcg cgctgatgat cacctacccg agcacccacg
     4741 gcgtcttcga ggccggcgtc gtcgagatct gccgcctcgt ccatgaggcc ggcggccagg
     4801 tctacatgga cggcgcgaac atgaacgcgc aggtcggcct gtcgcgaccc ggcgacatcg
     4861 gcgccgacgt ctgccatctc aacctgcaca agacgttcgc gatcccgcac ggcggcggcg
     4921 gcccgggcat gggcccgatc ggggtcgcgg cgcacctccg ctcgttcctg cccgggatcg
     4981 gcctggacga ggttgggcgg gtctcgtcgg cgccgttcgg cagcgcgagc atcctcccga
     5041 tctcctgggc gtacatcgcg atgctcgggg ccgagggcgt ccggcggtcg acgcaggtcg
     5101 ccatcctgaa cgcgaactac ctggcgaagc ggctcggcga cgccttcccg gtgctgtacg
     5161 tcggggatga gggcaccgtc gcccacgagt gcatcctcga ttgccggccg ctgaccgcgg
     5221 aaagcggcgt gaccgtcgac gacatcgcga agcggctcat ggacttcggc ttccacgcgc
     5281 cgacgatgag cttccccgtc gtcggcacgc tgatgatcga gccgaccgaa tcggagtcga
     5341 aggccgagct ggaccggttc gccgacgcgc tcctgcagat ccgggccgag atcgcggacg
     5401 tcgaagccgg tcgcgtcccg gtggagtcgt cggcgctccg gaatgcgcca catacggcca
     5461 ccgacctgct gcgcgatgag tgggcgcggc cgtacacccg tgagcagggc gcgttcccgg
     5521 ccccctgggt tcgccaccgc aagtactggc cgccggtccg ccgcatcgac aacgtgtacg
     5581 gcgatcggaa tgtcgtctgc gagtgcctgc cggtcgaggc gtacgcggag gcgtctgggg
     5641 aaacccagct gctcatggtc gccgggtagc gacccctctg cctcgatgtc cccgatgcac
     5701 gacgcgccgc tggtccatgc cgaggatcgc gcgacctggc gggcctggct agcagcgaat
     5761 cacgccacgg agacgggcgt ctggctcgtg agctggcgcc gcggccacgg gccgagcgtc
     5821 ccgtacgagg aggcggtcga ggaggcactc tgcttcggct ggatcgacag ccagggcggc
     5881 aacctcgacg cgacccgatc caggcagtac tacgcgccgc gcaacccgac gtccggctgg
     5941 gccgcgacga acaaggcccg ggtggagcgg ctgatcgccg acggcctgat ggcgccggcc
     6001 ggactcgcgg cgatcgagcg cgcgaaggtc aacggcgcct ggacgctgct cgacgacgtc
     6061 gagcgcggaa tcgtccccga cgatctcgcc gcggcgctcg ccgagcaccc gccggcggcc
     6121 tcgcacttcg agtcgttccc gaagtcggcg aagcgcgcga tgctcgagtg ggtcaggcag
     6181 gcgaagcggc ccgagaccag ggccaaacgc gtgctcgaca tcgccgaacg agcggccgag
     6241 aacgtgcgcg cctacccacc gcccgagcgc cgctgagcct cagacgggct tgaacgcctc
     6301 gaacggctga ccgcacgcgg cgcagtgccg gatcgaccgg cactgggtcg ggccgaacgc
     6361 gctctccagc gtggtctgca acgagccgca gtaggggcag gcgaccggag cggcggccag
     6421 gtcgagcatc ggcagggcgc gtccgagcgg ggcggtcggt ggtggcgcga agccggattg
     6481 gcgcagtttc tcgcgacccg ccggcgagat ccgttccgtc gaccacgggg tcgcgaacga
     6541 gacgtcgacg cgcaccggcc ggccgaactc ggccagccga gctgccaccg tatccctgat
     6601 cacctcgagc gccgggcagc cgacgaacgt ggggagcacc tcgaccgcga tcaggtcgtc
     6661 gtcgaaggcg atctcgccga tcatcccgag ctcgacgacc gacacgttcg ggatctccgg
     6721 gtcggcgacc tcatcgaggg cggcaagcac ctccgaccgg ccgatcaagc ggggcgcggc
     6781 gatcatcacc acgtcgcgcc gtcctcggag cggcgcacgg aggtgaactc cccccagagc
     6841 cagcggaagc tgcccgagtg cccggttcgc ccgtcggcgg cgggcggcat ccgcggcggc
     6901 agcgcgagcc cgagcgggac gaggatggtc gagagatcgg cgcgccacgc tgcctcggcg
     6961 tcggcgacgg agcgcgacag gattcgggcc tcgagcaggg ctgcctcgcc ctcgagtggg
     7021 gtgaagacgg tcgatgcatc ggccgcgagc gccgtgagcg cggcctcgag ccggcgccgc
     7081 ggctcctcac cgccgcgcgc gaggcgatcg atccaggcca acgcgtgcat ccggtggtag
     7141 cgctcctcgc ggcgcagctt ctcgacgaga tcggcgagcg gcttgaagcc cggtccgatc
     7201 gaggcgaggc gggcctcgtc cgccagctcg tacaggaagc ggcgggcgat cgtgtccgcc
     7261 cagtccccgc gggcgtgatc gaggaggcga gcgtggcggt actcgtcggg ctcgcggtcg
     7321 taggccagcg catcctcgtc gcggccatcg gccagcacgc cggcgagcag ctggtacagc
     7381 gcgcgggcgt ggccgagctc gtcctgcgag atcgagctga tcgcgatgtc ctcctcgagg
     7441 agcggcgcga tgcccgtcca ctcggaatcc gtgaagccgc cgacgaactc gtcgtcagcc
     7501 atcgtcagga ggagctcggc aagggctgtg cgggtccgcg cgtcgatggg cgcttcggtc
     7561 gcagtcattg ggcgacccat ccgtcctgcc cgagcgtcag cgcgaagagc tgtgcccccg
     7621 cttcgtcaga tgccacgacc cgctctgctc gggcgccacc cggccaccac gccagccagt
     7681 ggccattgcc gaccgacgcc gtcaccttgc gcccgcccgg cacctcgatc acgacctgcc
     7741 tcgcctccgc gccgagcctc ccaccgagat acgacagggt cccaacgtcc gcgccgccgg
     7801 agccctcctc atcaatcgag atcggctgat cgagcaactc cagttggccc gccggctggc
     7861 tgccgccacc gcccccggtc ggggagatgt agcacgaccc gaccatcgtc ggtccctgga
     7921 cgagaaacgc ggcggtcgcc gaggtgcgcc ggtcctggag gatgacctcc agggcgacat
     7981 cttgctcgag ggcgggccgg cacgccgggc tctgtgcggc tttggcgctg agccccgcgt
     8041 cgggggccag cggcaccgcc tgccaggtcg agaggtcatc cggtgcgggc gcgaagatgg
     8101 cgccaatgcc gcaaccggcg accaggaacg cggcgacgca ggatgcggcg atcgtcctca
     8161 aggttgtccc gcccgacggc cgccagtgcg gtcgtgggtc tccgcgcgcg ccgcgggctt
     8221 cgacgtgccg gctcgctcgc gagcggcggc gagcttgtcg cgcatcacgt agccgcccgg
     8281 cttcttgaac gagcggtcga gcggcgggtc gagaaggtcc ggatcctcga ggacggcgat
     8341 gtcgccgcgc cggacgatcc acaggcggga gctctcgttc cgtcgcccga acagctcgcg
     8401 ggcgtagtgc atcgccagct cggcgtcggg ggccatcacg ttgccgccgt ggcgcatcgg
     8461 gtcgccatcc ttggcctgct ggaagacctc gtacgcctcg agctcgacac gcttcggagc
     8521 ggcgcgcgcg gccatcacgc agccgccatc gatgccaatc cgaacaccgt ctcgcgcacc
     8581 cagcgggtgt cctcgcggtt gagccgtcgg aactcgagcc gctcctggga cttcgggccg
     8641 tggccggtga ccaccgagcg cagctcctcc cacgacggct ccgtgtacga ccaggtcttc
     8701 gtcgcctcgt cgaggcgcag ctcgggatcc gggatcgtca gcccgagctc gaggaggcgg
     8761 ggtacgtaga tcgtcaggaa ctcctggcgg agctcctcgg aggttttcgc cttgatccgc
     8821 cagtgcaggt cgcggtcctt ggcccggtcg ccccgcggcc cgtgcatctg catgagcggg
     8881 ccccaccaac gatcgagcgc ctcctggacg agggcacgct gggaatccgt gccggtcatc
     8941 agcgtcacca cgacatcacg cccatgcatg atgtggaccg actcctccca gcagatcttc
     9001 tgcatcgtcc gcgcgtacgg cgcgtagctc gagtcacgca gcgcctgctg ggcgacgatc
     9061 gccgcggcgt cgacgagcca ggcgatgatc ccgatgtcac cccagctctt cgtcgggtag
     9121 tggaagacg
//
LOCUS       DATBSW010000134         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_187638, whole genome shotgun sequence.
ACCESSION   DATBSW010000134 DATBSW010000000
VERSION     DATBSW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187638"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VIF63_03715"
     CDS             complement(<1..309)
                     /locus_tag="VIF63_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8238521.1"
                     /translation="MAARERPPRRARKLAMVALFEAEFPPQQADAALTRLAAEWGAEP
                     EVVAHAREIVAGVRRHQAALDAEIATRAPKIPVSELGRIERTILRSALFEVLYSAAIP
                     S"
     gene            complement(310..1395)
                     /gene="plsX"
                     /locus_tag="VIF63_03720"
     CDS             complement(310..1395)
                     /gene="plsX"
                     /locus_tag="VIF63_03720"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY8238522.1"
                     /translation="MRIAVDAMGGDLAPREIVRGAINYAATHADEVILVGDVPRIERE
                     IDEYGRGRPASVSFVDAPEVVEMGEAPAAGVRRKRSSIRVATDLVRDGAADAVVSAGS
                     TGASMAAAVFRLGRVEGIDRPALPAYFLTPSGPLVLLDVGANVDSDAQNLVQFAAMGS
                     LYAERVLHVTNPRVALLNIGEEPEKGDALTREAYGRFQGSDLNFVGNVEPHELASHPA
                     DVVVCDGFVGNVVLKLTEGLTSYIFRSIREDLLRGPVAPIAVLALRPGIDRLRHRFDY
                     EQFGGAPLLGVRGVSIVTHGRAKARMVENAIRVGSEAAAADIPAVIADWSRRHPALYA
                     GSPSANGAENSAPAPDEPAAVPIEPAP"
     gene            complement(1407..1610)
                     /gene="rpmF"
                     /locus_tag="VIF63_03725"
     CDS             complement(1407..1610)
                     /gene="rpmF"
                     /locus_tag="VIF63_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY8238523.1"
                     /translation="MGVPKRKVSKSRKGERRAHLALRAPTLVECDHCHELKRAHTVCP
                     TCGWHNGREVVPPPAPTPTESGR"
     gene            complement(1683..2207)
                     /locus_tag="VIF63_03730"
     CDS             complement(1683..2207)
                     /locus_tag="VIF63_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY8238524.1"
                     /translation="MTRLNVAGLLREPAGTSRTVELGSLEVMTEAALPRRVLGTIRLQ
                     RTNRGIVASGRARTLARWTCIRCLDEFDEPVEVEINEEFLPMDPTTGAVVVVDAADVQ
                     VARVDAHHEIDLAGVVNDELTLAEPMHPLCRPDCGGLCPICGLRRDDGVHDHPDTEID
                     PRLAGLADWKPPAE"
     gene            complement(2204..2689)
                     /locus_tag="VIF63_03735"
     CDS             complement(2204..2689)
                     /locus_tag="VIF63_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238525.1"
                     /translation="MLVATGKRVPLTNNVVVDQGAVLELVDQIRVAMPEEVRQAQRIT
                     GEAERIVEHAREESDAIIARAQEQAAHMLEERELVRLAQQRAGEILEQATREAGDVRR
                     GADEYAAGVLIRLEGEVTKALTSIKHGIEMLDQRRGPTTIDAIPVQPEADRAIGTPVS
                     G"
     gene            complement(2816..>3235)
                     /gene="coaD"
                     /locus_tag="VIF63_03740"
     CDS             complement(2816..>3235)
                     /gene="coaD"
                     /locus_tag="VIF63_03740"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8238526.1"
                     /translation="LFDRLVIGVLSNPKKAPLFSADERVALIRAEIRDLGESVTVEAF
                     EGLTVEFAARHDAGFVIRGLRAISDFEAELQMAHTNRRLAPSIDTAFLMTALEFGYVS
                     STLAKEVATFGGDVSAMVPPGVAAALKDRLEKAPDHR"
BASE COUNT          458 a         1145 c         1140 g          492 t
ORIGIN      
        1 gctcggaatc gcggcggagt atagcacctc aaatagggca ctcctgagga tcgtccgttc
       61 gattcggccg agttcggaga ccgggatctt cggcgcccgg gtcgcgatct cggcgtcgag
      121 cgcggcctgg tgacggcgca cgccggccac gatctcccgc gcgtgggcca ccacctccgg
      181 ctcggcgccc cactcggccg cgagccgggt cagggcggcg tccgcctgct gaggcgggaa
      241 ttcggcttcg aacagggcca ccatcgccag cttgcgggcc cgtctcgggg gccgctcgcg
      301 tgcggccacc tagggggccg gttcgatggg aacggcggcc ggctcgtccg gcgccggcgc
      361 cgagttctcg gcaccgttgg cggacggcga tccggcgtac agcgccgggt gccgtcggct
      421 ccagtccgcg atgacggccg ggatgtcggc cgccgccgcc tcggacccga cgcggatcgc
      481 gttctccacc atccgtgcct tggcccgacc gtgggtcacg atgctcaccc cacggacgcc
      541 gaggagtggg gcgccgccga actgctcgta gtcgaaccgg tgacggagcc ggtcgatgcc
      601 cgggcgaagc gcgaggaccg cgatgggcgc gaccgggccg cgcaagaggt cctcacggat
      661 ggagcggaag atgtacgagg tcagcccctc ggtgagcttg aggacgacgt tgcccacgaa
      721 cccgtcgcac acgaccacat ccgccggatg gctggccagt tcgtgcggct cgacgttgcc
      781 cacgaagttc aggtccgatc cctggaaccg gccgtaggcc tcccgagtca gcgcatcgcc
      841 cttctccggc tcctcgccga tgttcagcag cgcgacccgc gggttggtca cgtggagcac
      901 gcgttcggca tacagcgagc ccatcgcggc gaactggacg aggttctgcg catccgagtc
      961 gacgttggcg cccacgtcga ggaggacgag ggggccggag ggcgtcagga agtaggcggg
     1021 cagggccggt cgatcgatcc cctcgacccg cccgaggcgg aagacggcgg cggccatgct
     1081 ggcgccggtc gagccggcgc tgaccaccgc atcggccgcg ccgtcgcgga cgagatcggt
     1141 cgcgacccgg atcgaggagc gcttccgtcg aacgccggcg gccggtgcct cccccatctc
     1201 gacaacttcg ggcgcatcga cgaaggacac gctcgccgga cggccgcggc cgtactcatc
     1261 gatctcgcgc tcgatgcgtg gcacgtcacc cacgaggatg acctcgtcgg cgtgggtggc
     1321 ggcgtagttg attgcccccc ggacgatctc gcgcggcgcg agatctccgc ccatcgcgtc
     1381 gaccgcgatg cgcatcgggc cgaccgtcag cggccggact cggtcggggt gggcgccggc
     1441 ggcggcacca cctcgcggcc gttatgccag ccgcaggtcg ggcacacggt atgtgcgcgc
     1501 ttcagctcgt ggcagtggtc gcactcgacc agggtcggcg ctcgcaacgc gagatgggcg
     1561 cgacgttcgc ccttccggga tttcgagacc ttgcgcttgg gaacgcccac gcggctctcc
     1621 tgtgcagaat cgggatgagg aggccgcagt atagcggtgg tccggggcgt ggaccacatg
     1681 cgtcactcgg cgggcggttt ccagtcggcc agcccggcca gccgcggatc gatctcggtg
     1741 tcgggatggt catggacgcc gtcgtcgcga cgaaggccgc agatcgggca caggccgccg
     1801 cagtcgggac ggcacagcgg gtgcatcggc tccgccaggg tgagctcgtc gttcacgacc
     1861 ccggccaggt cgatctcgtg gtgggcatcg acccgtgcca cctgcacgtc cgcggcgtcc
     1921 accaccacga ccgcgccggt cgtcgggtcc atgggcagga actcctcatt gatctccacc
     1981 tccaccggct cgtcgaactc gtcgagacag cggatgcagg tccagcgggc cagggtccgg
     2041 gctcggccgg aggccacgat gcctcggttc gtgcgctgga ggcggatggt gcccagcacg
     2101 cggcgcggca gcgccgcttc ggtcatgacc tcgagcgagc ccagctcgac cgtccgcgac
     2161 gtgcccgccg gttcccggag gaggcccgcc acgttcaggc gggtcacccg gatacggggg
     2221 ttccgattgc gcggtcggct tcgggctgga ccgggatggc gtcgatggtg gtcggcccgc
     2281 gccgctggtc gagcatctcg atcccgtgct tgatgctggt cagcgccttg gtgacctcac
     2341 cctcgagccg gatcaggacg cccgcggcgt actcgtcggc accgcgacgg acgtcgcccg
     2401 cctcgcgagt cgcctgctcg aggatctccc cggcccgctg ctgagccagc cgcacgagct
     2461 cacgctcctc gagcatgtgc gcggcctgct cctgggcgcg ggcgatgatt gcgtcgctct
     2521 cctcgcgagc gtgctcgacg atgcgctccg cctcaccggt gatccgctgt gcctggcgaa
     2581 cctcttccgg catggcgacc cggatctggt ccaccagctc gagcactgcc ccctggtcga
     2641 ccacgacgtt gttcgtgagc ggaactcgct tgccggtcgc gaccagcacc tcgagacgct
     2701 cgacgaggaa caggatgtcc gtggcgatga cctccgtcga tggttgtcgc cgatactaca
     2761 ccgccagcac gttgtcgtac aacgtcatac cgcccctcgc ggcccgcacg atcgcctagc
     2821 ggtggtccgg tgccttctcc agccggtcct tgagcgcggc ggccacgccc ggcgggacca
     2881 tggccgacac atcgccgccg aaggtcgcga cctccttggc gagcgtggac gacacgtatc
     2941 cgaactcgag cgcggtcatc aggaacgcgg tgtcgatcga cggggccagc cggcggttgg
     3001 tgtgcgccat ctggagctcg gcctcgaagt cgctgatcgc acggagccct cggatcacga
     3061 atcccgcatc gtggcgggcc gcgaactcga cggtcagccc ctcgaacgcc tcaaccgtga
     3121 ccgattcccc caggtcccgg atctccgccc ggatgagcgc gacgcgctcg tcggccgaga
     3181 acagcggcgc cttcttcggg ttgctgagca caccgatgac gagtcggtcg aacag
//
LOCUS       DATBSW010000135         4877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_681090, whole genome shotgun sequence.
ACCESSION   DATBSW010000135 DATBSW010000000
VERSION     DATBSW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681090"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VIF63_03745"
     CDS             <1..674
                     /locus_tag="VIF63_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8238527.1"
                     /translation="ADGLPDRALEGLRGDDLAALADSAASGPAPIELVRTSAVLGHAA
                     ALNIGIRRSTAPIVIVLDPSVVPAADIVTPLVRALDDPGVAVAGPFGLVSADLRRFEE
                     VVAAPAASNRESDKPAPARRAAAAIQGYLMAFRRADAIERGPLDEGFRFYRNLDIWWS
                     LVLRDEGEDAPPRRAVVVPGLSLTRGEPRAWASTAPAERDRLSKRNFYRVLDSFRTRP
                     ELAVE"
     gene            complement(917..2581)
                     /locus_tag="VIF63_03750"
     CDS             complement(917..2581)
                     /locus_tag="VIF63_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238528.1"
                     /translation="MNLRLRDNLTRGLVPIEPIDGERIRMYSCGPTVYRFAHVGNLRT
                     FMLADLIRRVVLYHGVDVLQVQNITDVGHLRADAAGREVDPMLVAAGVEGRPSAEIAD
                     AYEAAFHADAALVNILPAHAFPRATEHIDDMLALARQLVDLGHAYVTEIGNVYYSVAS
                     FPSYGALSGNTLDSLRAGHRTEGEVEADKRDAADFALWRASGPDSTRDLHWPSPWGDG
                     FPGWHLECSAMALRHLGPTFDIHTGGEDNVFPHHEDEIAQSAPIVGGPPARAWVHGAH
                     LLMSGRKMSKSAGNFQRITELVESGLDPLAFRYLCLTARYAKKLNLSDASLAGAAGGL
                     ASLRAELAALGPAPASGPWAAPAVLRAGASPARPVGLAAGLTGHGGAGEPSVRPGIDG
                     SAPVPSAGSAPVPSAGSDPVLADRAGIPLAPLSEAGRALHDSFVEAIDADLDMPTALR
                     IARETLRAPLPPDERRWLVLDFDFVLGLDLDRSVPSTGNAGSGWAGVGAALALSAAAQ
                     RLLADRTKARAAQDWAAADGLRDELHKLGIEPIDRADGTSDWRHIP"
     gene            2691..2852
                     /locus_tag="VIF63_03755"
     CDS             2691..2852
                     /locus_tag="VIF63_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238529.1"
                     /translation="MRQWAAVVALLLGLAGNGYVAAARLRLPIAIERPLETTTTDGRT
                     KRTLDWWRA"
     gene            2840..4429
                     /locus_tag="VIF63_03760"
     CDS             2840..4429
                     /locus_tag="VIF63_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8238530.1"
                     /translation="MARLTLRSPRRRRLGAFLGSLAILAVVVSAGAPRDAVAGENDEV
                     RILAGEPSTFDPAATGDATTAAITAQVYETLTTYDATLTLQPALAASWDVAPDGLSVV
                     FHMRPSLQFSDGSSLTARDVVGSWLRIIDPRTPSPLAALMIDVKGARAYLSGQTSDPA
                     AVGIRANGDDVEVELERPGADFPAIVSSPTFGIVPTPVWQDGRDLFGPGAPGSGGFTV
                     EAVNADEITLTRNERYWAGAPAIQTVHLLLDLGGQNPVAAFDAGDLDYTEISSIDAPW
                     IPYDRDLGPQLREVSSLSLTYLGIDTTTAPFDDVRVRQALGAAVDWARIVPLGASEGA
                     TPAISMVPPGIPGGGDKNWLPAHDPELARQLLTEAGYPGGAGLPPIRFAAGLLPLADA
                     IAADLERELGMQVELESLDDHLGRLDSDPPNLWVTGWIADYVGPNDFLGVLLESDSGN
                     NDGRWSSPAFDKAIAEALATRDSAAAQAAYERAMAEIQAEVPVVPLYLSTNWFLSREG
                     LLGAGDNGLGILRVAGMSWAR"
     gene            4426..>4877
                     /locus_tag="VIF63_03765"
     CDS             4426..>4877
                     /locus_tag="VIF63_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238531.1"
                     /translation="MSRRQAARRAPRRAGAGPGPRFAAALAIALIAGLAAAPAALAAD
                     PTFGPATATAKFGESIQVEQQATLPEGVKRVEALVRAGPNARTFVAEIPAPGAGPTTL
                     RYTNPTPNGALFPNTRVELGFRVTLADGRSFDGPTTTVLYEDDRFTWRS"
BASE COUNT          673 a         1767 c         1753 g          684 t
ORIGIN      
        1 ttgccgacgg cctcccggat cgcgcgttgg aagggctccg cggcgacgac ctcgccgcgc
       61 tggcggactc agcggcgtcc ggcccggcgc cgattgagct cgtgcggacg agtgccgtgc
      121 tcggtcacgc cgcggcgctg aatatcggca tccggcgttc gaccgcgccg atcgtgatcg
      181 tgctcgaccc gagcgtggtc ccggctgccg acatcgtcac gccgctcgtc cgggcactcg
      241 acgatccagg cgtcgcggtc gccggtccct tcggcctcgt ttcggctgac ctgcgccgct
      301 tcgaggaggt ggtcgcggcg ccggccgctt ccaatcgcga gagcgacaag cccgccccgg
      361 cccggcgcgc cgccgcggcg atccagggct acctgatggc cttccgccgc gctgacgcga
      421 tcgagcgcgg tccgctcgac gagggcttcc gcttctaccg caacctcgac atctggtgga
      481 gcctcgtcct ccgcgatgag ggcgaggatg ccccgccgcg tcgcgccgtg gtcgtgcccg
      541 gcctgtcgct gacccgcggc gagccgcgtg cctgggcgtc aaccgcgcct gcggaacgcg
      601 atcgcctgtc caagcggaac ttctaccgag tcctcgacag cttccgcact cggccggagt
      661 tggcggtcga gtagatcctg ctcggatcaa ccagggtcga atcgaccccg gcccaacggg
      721 cgaccaacag gccccagcgt ttctcacgac gccggtgatg cctgtgggtg catcagacgg
      781 ggacggcgat ccgggccgat gctcggaatc gcaagccacc actgccgtga ttagcccaga
      841 ggcatcacag gccacgaagg aaatgtcgcg tggctttcta cactcgccga aggagcgagc
      901 ggagcgcccc cagcggctac gggatgtggc gccagtcgct cgtgccgtcg gcgcggtcga
      961 tcggctcgat gccgagcttg tgcagctcgt cgcgcaaccc atcggcggcc gcccagtcct
     1021 gcgccgctcg cgccttcgtt cgatccgcga gcagccgttg cgcggcggcg gacagggcca
     1081 gcgcggcccc gacgcccgcc cagccggagc cggcgttgcc cgtggacggg acagatcgat
     1141 cgaggtcgag tccgagcacg aagtcgaagt cgagcacgag ccagcgccgc tcgtcgggcg
     1201 ggagcggcgc gcgcagcgtc tcccgcgcga tgcggagcgc ggtgggcatg tcgaggtctg
     1261 cgtcgatcgc ctcgacgaac gaatcgtgga gcgcgcgacc cgcctccgag aggggcgcca
     1321 ggggaatgcc cgcgcggtcg gcgaggacag ggtcggagcc cgcgctcggg acgggtgcgg
     1381 agcccgcgct cgggacgggt gcggagccgt cgattcccgg cctgacgctt ggctcccctg
     1441 cgccaccgtg cccggtcaaa ccggcggcga gacccactgg ccgcgcgggc gaggcgccgg
     1501 cccggaggac ggcgggcgcc gcccacggtc cggacgccgg tgcggggccc aacgcagcca
     1561 gctccgcccg cagcgacgcg aggccccccg ccgcgcccgc gagcgaagcg tcggacaggt
     1621 tgagcttctt cgcataccgc gccgtcaggc ataggtagcg aaatgcgagg ggatcgagcc
     1681 cggactcgac cagctccgtg atccgttgga agttgcccgc cgacttcgac atcttccggc
     1741 cgctcatgag caggtgcgcg ccgtggaccc aggcgcgcgc cggcgggccg cccacgatcg
     1801 gagcggactg cgcaatctcg tcctcgtggt gggggaagac gttgtcctcg ccgccggtgt
     1861 ggatgtcgaa ggtcggcccg aggtggcgca gcgccatcgc ggagcactcc agatgccagc
     1921 ccgggaagcc gtcgccccac gggctcggcc agtgcaggtc gcgagtcgag tcggggccgg
     1981 atgcgcgcca cagggcgaag tcggccgcgt cgcgcttgtc cgcctcgacc tcgccctcgg
     2041 tccgatggcc ggcccgcaac gaatcgagcg tgttcccgga cagcgcgccg tacgacggga
     2101 agctcgcgac cgagtagtac acgttcccga tctcggtcac gtacgcgtgc ccgaggtcga
     2161 cgagctgccg agccagggcg agcatgtcgt cgatgtgctc ggtcgcgcgc gggaaggcgt
     2221 gcgccgggag gatgttcacg agcgcggcgt cggcgtggaa tgccgcctcg tacgcgtccg
     2281 cgatctcggc cgacggccgg ccctcgacgc cggcggcgac gagcatcggg tcgacctcgc
     2341 gtcccgccgc gtcggcgcgc aggtggccga catcggtgat gttctggacc tggagcacgt
     2401 cgacgccgtg gtagagcacg acgcggcgga tcaggtcggc cagcatgaac gtccgcaggt
     2461 tgccgacatg cgcgaagcga tagaccgtcg ggccgcacga atacatccgg attcgctcgc
     2521 cgtcgatcgg ctcgataggg acgaggcccc gggtcaggtt gtcgcgcagc cggagattca
     2581 cgggcggagc ctaacgagcg gttcggtttc gcgttctcac tagtcggggc tccgcgaacc
     2641 gcttcgttcc cccggcgatg cgacgcgggg ccttccccca tactccggca atgcgtcagt
     2701 gggctgccgt cgtcgccctc ctgctcggcc tcgccggcaa cggctacgtc gcggcggcgc
     2761 gactccggct cccgatcgcg atcgagcgcc ccctcgagac cacgacgacc gacgggcgca
     2821 cgaaacggac gctcgactgg tggcgcgcct gaccctgcgc tcgccccgtc ggcgtcgcct
     2881 cggggccttc ctcggttcgc tggccatcct cgccgtggtc gtgtccgccg gtgcgcctcg
     2941 ggacgcggtc gcgggcgaga acgacgaggt caggattctc gcgggcgagc cttcgacgtt
     3001 cgacccggcg gccaccggcg acgcgacgac tgccgcgatc accgcccagg tgtacgaaac
     3061 gctcacgacc tacgacgcga cgctgaccct ccagccggcc cttgccgcaa gttgggacgt
     3121 cgccccggac ggcctgagcg tggtcttcca catgcgcccg agcctgcagt tctcggacgg
     3181 atcgtcgctg accgcgcggg acgtcgtggg cagctggttg cggatcatcg acccacggac
     3241 accgtcgccg ctggcggcgc tgatgatcga tgtgaagggc gctcgcgcgt acctttccgg
     3301 gcagacgtct gacccggccg ccgttggcat ccgcgcgaac ggcgacgacg tcgaagtcga
     3361 gctggagcgc ccaggcgccg atttcccggc gatcgtgtct tcgccgacgt tcgggatcgt
     3421 gccgaccccg gtctggcagg atggccggga cctgttcggg ccgggcgcgc ccggcagcgg
     3481 cgggttcacg gtcgaggcgg tcaacgccga tgagatcacg ctcacccgca acgagcgcta
     3541 ctgggccggg gcgccggcga tccagacggt ccacctgctc ctcgatctcg gtggacagaa
     3601 cccggtggcc gcattcgacg cgggcgacct ggactacacc gagatctcga gcatcgacgc
     3661 gccgtggatc ccgtacgacc gagacctcgg gccgcagctg cgcgaggtct cgtcgctgtc
     3721 gctgacgtat ctcggcatcg acacgacgac tgcgccgttc gatgacgtgc gcgtccgcca
     3781 ggcgttgggc gcggcggtcg attgggcgcg gatcgtgccg ctgggcgcgt ccgagggcgc
     3841 cacgccggcg atcagcatgg tgccgccggg catccccggt ggcggcgaca agaactggct
     3901 ccccgcccac gacccggagc tcgctcgaca gctcctcacc gaggccggct acccgggcgg
     3961 cgccggcctg ccgccgatcc ggttcgcggc cggcctgctg ccgcttgccg acgccatcgc
     4021 ggccgatctc gagcgcgagc tcgggatgca ggtggagctc gagagcctcg acgaccacct
     4081 cggccgactc gactcggacc cgccgaacct gtgggtgacc ggctggatcg cggactacgt
     4141 tggcccgaac gacttcctgg gcgtgctcct ggagagcgac agcggcaaca acgacggccg
     4201 ctggtcctcg cccgcgttcg acaaggccat cgcggaggcg ctcgcgaccc gcgactcggc
     4261 ggccgcgcag gccgcgtacg agcgcgcgat ggccgagatc caggccgagg tcccggtcgt
     4321 gccgctgtac ctgagcacga actggttcct gtcccgcgag ggcctcctcg gggcggggga
     4381 caacgggctc ggaatcctca gggtggcggg catgtcgtgg gcgcgatgag ccggcgacag
     4441 gcggcgcgac gagcgccgcg acgcgcgggc gccggcccgg gtccgcgatt cgccgctgcc
     4501 cttgccattg cgctgatcgc cggccttgcc gcggctcccg ccgcgctcgc cgccgacccg
     4561 acgttcgggc ctgcgacggc caccgcgaag ttcggcgaat cgatccaggt cgagcagcaa
     4621 gccacgctgc ccgagggcgt caagcgcgtc gaggcgctgg tgcgcgccgg gccgaacgca
     4681 cgcacgttcg tggccgagat cccggcgccg ggcgccggcc cgaccacgct gcgctacacg
     4741 aacccgacgc cgaacggcgc gctgttcccg aacacacggg tcgagctcgg cttccgggtc
     4801 accctcgccg acggccgatc gttcgacggc ccgacgacaa ccgtgctgta cgaggacgat
     4861 cgcttcacct ggcggtc
//
LOCUS       DATBSW010000136        12299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1245317, whole genome shotgun sequence.
ACCESSION   DATBSW010000136 DATBSW010000000
VERSION     DATBSW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245317"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VIF63_03770"
     CDS             <1..557
                     /locus_tag="VIF63_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8238532.1"
                     /translation="NADALVCSGYKWLSAHGGVALLAIGDEFINRIPALVGWKGAENP
                     FALAPTTLALATDARRFELSTIAYSSAAGLTTSAKLLSDVGIDVLDRNARLLSRELVD
                     AVAPLGWTPFRDPDDAGATGHIVSLRHGTLAAPAVRERLAENHHVNVSARLGRIRVSL
                     HGYNDSDDVAGLVAALETTAASLA"
     gene            complement(561..1439)
                     /locus_tag="VIF63_03775"
     CDS             complement(561..1439)
                     /locus_tag="VIF63_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEY8238533.1"
                     /translation="MPRADGRPDLRSALDGVRAFVLDADGVLMYRGAPIPGSPEAVVE
                     LRRRGLPYRVVTNFSSAHRSSLAAAFGKATGLAVDPAEIITASSAAAAYTAAHHPGKP
                     LLVLAAPDARREWAGQDLIEIGEADARSEPVAAVVIGDAGDDLSYANLDIAFRQLRNG
                     AEFVAMHRNPWWMTPKGFTLDAGAVVAGLEFALGRRAVICGKPSPVVFRQAVGELRAE
                     LAARPGGSGGPRLRTGDVAMVGDDPRADIAAARRVGLRGVLVLTGKVSGEEAEASGIG
                     SEFVAPNLGSVVAALG"
     gene            1465..2247
                     /locus_tag="VIF63_03780"
     CDS             1465..2247
                     /locus_tag="VIF63_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY8238534.1"
                     /translation="MGLLEGKKALIFGVANDHSIAWGIAQALHEQGAILGFSSVESLV
                     ERRVRPLATSIGSTFVEPCDVQSDEQIAAVMSKWRDTYGEIDVLVHALAYAKREDLDG
                     EFANTSRDGFALAMDVSAYSLVALVREARALMHPGSSVLTLSYYGAEKVVAHYNVMGV
                     AKAALEASVRYLAADLGPSGIRVNAISAGPVRTLAAAGIAGFKGMYGSFRDLAPLRAN
                     ITPEDVGKTAIWLASDLSSAVTGEVVYVDGGFNIMGVPLAEG"
     gene            complement(2253..4370)
                     /locus_tag="VIF63_03785"
     CDS             complement(2253..4370)
                     /locus_tag="VIF63_03785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8238535.1"
                     /translation="MDRPPTPPSPIARPTYRPLERFLSAAGDSAAGSGWTRSRLAARI
                     RSDTYLLLAIAIALPVVAMEGIADLSQRPMGLLAPLIFVAAQLWLTTLRKPPEWLGTA
                     RLALCLAFIGLANLWIDPSGTWPLSALAIPVVALAAAGGGRAGVLVAAAGMVLMLVPL
                     GLPGLDVTDRQEVFAVAMAAIVLSYGTRQIVAKLERSSNRLRRANLAARRHARQLAAV
                     EAVGSVLAREGPTKAALDRVMKVLEETFGYHYPSVYVWDGTALQLGAQRNYQSPIQTI
                     SPDRGILGRIYHSREAVFLPDARSDPDFLSADSNVVSEIGVPLMGDGELLGILNVETS
                     GDRRLRDEDFGLMQIVGDRLAAALALGRERQKLTERAALLDRLTTFATVLGSSLDPAT
                     MDEEVAIGALRVIPADALLLVNRDDATGAYVVNAVAGGDTMVIGQALEPGEGITGRAI
                     STRAVVVEDRLERARYSRAVAGLQLADVLAAISAPMVIGDEVAGVVTWLRRDLGTPFT
                     TQEKEIAGLLAGKVGLALANARLHQTAQDAAITDPLTGLRNRRYFDATLERDDAVRRR
                     IPAEKRPMRSAILFDLDHFGQVNKKFGHQVGDRVLRLFADTLRARARESDLVARYGGE
                     EFVVILENATRDDAIRIAEDVRTAFGKLLVESSGGDSIRTTVSAGCATLEPWEVEASL
                     LLERADVALAMAKAGGRNQVVAA"
     gene            complement(4480..6699)
                     /locus_tag="VIF63_03790"
     CDS             complement(4480..6699)
                     /locus_tag="VIF63_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238536.1"
                     /translation="MEGRQLRDRDAIALDLVGVIGLSAFLLGLVMLDTSEKLGFDAVE
                     ALNGVLSQLSVTLPGALLILAATSLELATGLVLARAARGRAFDSVSESLLAAMVAAVL
                     KDTLLLGTVAGFGLFRAPVLIGIDVAIIAGALLLAPISRHVRPLTAFASWRDALDSIG
                     SWPVAALVGIVWIGPVVLQLASPVVPFIDVLPNYVGPVEHLRTFGWFSPLSETQSPII
                     GPSRTVLGYDGLLGALATMTNLPGGLAIAAFIAPQTVLVAAGAYRLASALRDRTDVPI
                     GAWALLAFALSQSFARLADARGTVVVVPLICLGLALAADSLRRMEDPWRIGRGAVIGL
                     ALGAATLVHPVIAFFAIVTVGIAGLARPRDLGADAFVATLTAGLIALPQLATMLGASL
                     PTLALGVWLPVAVAIGLAAGRLIARRERLRDGIVRAAEIGRFVAVVVVVGGIAIAFAA
                     SILDVERVPKAFDPTLTLVLESSGLLLVVLIAGALAGSTGAPSPLVWAGALVGVVAVL
                     LANVLPDDLGFLGDALRFEVPKSVHYWLSSIAAAGAAAALAYLWATDRLPWLVRAAGL
                     AAFVAIAALPVRPEPIDSRHLGEHRWSETFAIDLKYADSGYWVGFPDSRHIVDEPRRA
                     ILDAVRAEIDAGRLQHDTPVLHIAKSFQQWVATPLGVFDGVTETFISLDPEVTHQTVG
                     GRLEGFEAMPSYLAAPVFPYVVLEPDGVPEGTREQLIAAGYASIFANDQGEVFVLPN"
     gene            complement(6753..6828)
                     /locus_tag="VIF63_03795"
     tRNA            complement(6753..6828)
                     /locus_tag="VIF63_03795"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6793..6795),aa:Gly,seq:tcc)
     gene            6908..7669
                     /locus_tag="VIF63_03800"
     CDS             6908..7669
                     /locus_tag="VIF63_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEY8238537.1"
                     /translation="MTGRRISRLGLAALLAGLVVGPTAQHVAAADLVLPDLAMMAPFD
                     FRIEISPSGRKLLRFSTVAVNIGKGPFQLYGHDADGAKIGDTLQITQLIKRTDGTYMP
                     RDSTATMTWSGDGHNHWHINGYQRFVLNKLDGTHLRYVKKTGFCAFDSYRYGSALPAF
                     YTWDAYACRTAPNGEVPMGTSRMWGDIYKSTIAFQWIDITGLASGTYRLNISVDSPRT
                     TGGRFLESNESNNLAWAKISIGKTTVTVISKSAKP"
     gene            7723..9567
                     /locus_tag="VIF63_03805"
     CDS             7723..9567
                     /locus_tag="VIF63_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8238538.1"
                     /translation="MTVESPPRSCPRCEAAVAADARFCASCGQALAESTSGDEALHTR
                     LTATAPEPLIQKMRAAKLTGERKPVTALFADVVGSTTLAEQIDPEDWTAIINEAFDLM
                     SRAVFRYEGTIAQLQGDAMLAFFGAPIAHEDDPERAIFAAIDMLAAIEEFAAQLRTSH
                     GIEFRIRAGINSGPVMVGNIGSDLRYEYTALGDAVNVAARMQSSAQPGTIVITEMTRR
                     LTGDTFEVDDLGEIDVKGKTDKIHAFRVVGRKSAPARRRGLESVGLDSPMVGREAQLG
                     RLVGLLDVIRSGRGRAAFVIGEPGIGKSRLLAEFKRITLATDAAPTRWIEGRCLSYGR
                     GLPYHLLIDLLRSILDVPFSASETEARAAIDRTLERLLGADPAEVADTAPYLAHLLDL
                     PLLPGEESVTKVVPDVLQSRYIAAVHRLLRSIAAERPLVLVCEDIHWADPASVAVAGQ
                     LMPLASQLPILFVGAMRAEMDSAGWLLLQQARDLFGNDLTEIQLEPLTEAEGRALVSN
                     LLEIESLPERTRDLIMARAEGNPFFVEEVVRMLIERGVIVAQGDRWVATDEAGGVEIP
                     ESLHGLLLARIDQLPDATKRSLRIAAVIGRQFPVRVLERVVAESVVTP"
     gene            9564..11135
                     /locus_tag="VIF63_03810"
     CDS             9564..11135
                     /locus_tag="VIF63_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238539.1"
                     /translation="MTTEKHADTDLDTDLATLTSKGLIKRAAELPELEYLFRHALVQD
                     AAYGSLLKQERRELHGLVGEALEELYPDRTGELAPVLAMHFEQAGETDKAIDYFAAGG
                     QHALQQNAIQEAYGAFDQASGLLARTPAPAPGAPAESPLRQRRRIEIKLGRAQAGYSF
                     LSPDQAFDSVEEIVAEAEELGDLELLARVHALIALGLMQSGVQPTEPRVARSLARIEE
                     IGIALGDPGIRAMPLAFVGLSQVFAGPVREGVAALEKAVPLMQDQKDSIGAAFARGGL
                     AMGYATLGEFDKADAAAANAKELAKEGDLIAQLDALIAESMVRAQQGRLDLAVPLARE
                     CVDRAESTGASACVLASSWILGDALHRLGKFADARDVLRRGADVALMVDRYVWRPTLQ
                     AWLRSAAAALGEVEEGDVDEALATARSIGNELGEAGILGKRAEAAASRSEFDSALADY
                     AATAAIFEAQGARPSLARVLQGWGETLRRAGRDAEATATLERAAALFDELGLTREAGA
                     VRTSITLGSTKLAFN"
     gene            complement(11216..>12299)
                     /locus_tag="VIF63_03815"
     CDS             complement(11216..>12299)
                     /locus_tag="VIF63_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238540.1"
                     /translation="DGDDQSTTVNTDFGTQLDLTVTDSEGNPVAGAAVTFAGPLSGAS
                     ASIVEPGPYLTDSDGNLVVTAHANSIAGGPYDLVATSGSGTATFHLTNLVGAAASITI
                     NAGDNQLATINTNFPTRLDLTVLDAGGNPVPNATVTFGGPLSGASASIVETGPYITDS
                     NGNLVVTARANGTPGGPYDFVASSGTASATFHLTNLPVVTAQLSANASCATFANATAT
                     TLTEITYSTNKTKINTLSESSFSYWVNVTAVAGSNTFTVNQSILSGNFSTLFGLANGN
                     GSGVFRSNCTGGPNATFSQSSTNAAAGTISVTFTAPTGGTYYINVKLSTRTVKGATPP
                     SPATVNYAFSTTGVPGTTRGLNLRRT"
BASE COUNT         1964 a         4230 c         4345 g         1760 t
ORIGIN      
        1 ggaacgcaga cgccctcgtc tgcagcggct acaagtggct gtcggcgcat ggcggggtgg
       61 ccctgctcgc aatcggcgac gagttcatca atcgcatccc ggccctggtc gggtggaagg
      121 gcgccgagaa cccattcgcc ctggcaccca cgaccttggc gctcgccacg gacgcccgcc
      181 ggttcgagct ctcgacgatc gcctacagct cggcggccgg cctcacgacg tcggcgaagc
      241 tcctgtccga tgtcggaatc gacgtgctcg atcggaacgc gcgactgctg tcacgggagc
      301 tcgtcgacgc cgtggcgccg ctgggctgga cgccgttccg ggatcccgac gatgcgggcg
      361 cgactggtca catcgtctcg ctccgccacg gcacgctcgc cgcgccggcg gtccgtgagc
      421 gtctggccga aaaccaccac gtcaacgtca gcgcgcggct gggccggatc cgcgtctcgc
      481 tgcacggcta caacgacagc gacgacgtcg ccgggctcgt ggccgcgctc gagacaaccg
      541 cggcgtccct ggcctgacgc tcagccgagg gctgcaacca ccgagccgag attcggtgcc
      601 acgaactcgc tgccgatccc gctggcctcg gcttcctcac cggagacctt gccggtgagc
      661 acgagcacgc cacgaaggcc gacacggcgc gcggctgcga tgtctgcccg tgggtcgtcg
      721 ccgaccatgg cgacgtcgcc ggtacggagg cgagggccgc cgctcccgcc gggacgagcg
      781 gccaactccg cgcgcagctc cccgaccgcc tgccgaaaca cgacgggcga cggcttgccg
      841 cagatgacgg ccctgcggcc gagcgcgaac tcgaggccgg cgacgaccgc tccggcatcg
      901 agggtgaagc ccttcggcgt catccaccac gggttgcgat gcatggccac gaactcggcg
      961 ccattgcgca gctggcggaa ggcgatatcg aggttcgcat aggacaggtc gtcgccggca
     1021 tcaccgatca cgaccgcggc gacgggctcc gagcgcgcat ccgcctcgcc gatctcgatc
     1081 aggtcctggc cggcccactc ccgtcgcgcg tccggggcgg ccaggacgag cagcggcttg
     1141 cccgggtgat gcgcggcggt gtacgcggcg gccgcagacg acgcggtgat gatctcggcc
     1201 gggtcgacgg cgagccccgt ggccttgccg aacgccgcgg cgaggctcga ccggtgggcc
     1261 gacgagaagt tcgtcacgac ccggtacggc agcccgcgcc tgcgaagctc gaccaccgct
     1321 tcgggcgacc ccgggattgg cgcgccgcgg tacatgagca cgccgtcggc gtcgagcacg
     1381 aacgcgcgca cgccatcgag cgcgcttcgg agatcgggac gaccgtcggc tcggggcacg
     1441 gccgcgatgg taggctccgg cgcgatggga ctgctcgaag gcaagaaggc gctgatcttc
     1501 ggcgtcgcca acgaccattc gatcgcctgg ggcatcgcgc aggcgctcca cgagcagggc
     1561 gcgatcctgg gcttctcatc ggtcgagagc ctggtcgagc gacgggttcg gccgctcgcc
     1621 acgtccatcg gatcgacctt cgtcgagccg tgcgacgtcc agtccgacga gcagatcgcg
     1681 gcggtcatgt cgaaatggcg cgacacatac ggcgagatcg acgtcctcgt tcacgccctg
     1741 gcctacgcga agcgagagga cctcgacggc gagttcgcga acacgtcgcg cgatggcttc
     1801 gcgctggcga tggacgtctc ggcctactcg ctcgtcgcgc tcgttcgcga ggcgcgggcg
     1861 ctcatgcacc caggttcgtc cgtgctcacc ctgagctact acggcgcgga gaaggtcgtt
     1921 gcccactaca acgtcatggg cgtggcgaag gccgcgctcg aggcctcggt ccgctatctc
     1981 gccgccgacc tcggcccgtc cggcatccgc gtgaatgcca tctcggcagg tccggtcagg
     2041 acgctcgccg cggcaggcat cgcaggcttc aaggggatgt acggatcatt ccgcgatctc
     2101 gcgcccctgc gcgcgaacat cacgcccgaa gacgtcggca agaccgcgat ctggctcgcc
     2161 tcggacctgt cgagtgccgt cacgggcgaa gtcgtctacg tcgacggcgg cttcaacatc
     2221 atgggtgtgc cgctcgccga aggctgaccg acctaggccg cgacaacctg gtttcggccg
     2281 ccggccttcg ccatcgccag tgcaacatcg gcacgttcca gcaggaggga cgcctcgacc
     2341 tcccacggct cgagggtcgc gcacccggcc gagacggtgg ttcggatcga atcgccgccc
     2401 gagctttcga ccaggagctt gccgaacgcg gtgcgcacgt cctcggcgat tcgaatggcg
     2461 tcgtcgcgcg tggcgttctc caggatgacc acgaactctt cgccgccgta gcgcgccacg
     2521 agatcgctct cgcgggcgcg cgctcggagc gtgtccgcaa agagccgcag cacccggtcc
     2581 ccgacctggt gaccgaactt cttgttcacc tggccgaagt ggtcgagatc gaacaggatc
     2641 gcggagcgca tcggacgttt ctccgccggg attcgacgac ggacggcgtc atcgcgctcc
     2701 agcgtcgcat cgaagtaccg ccggttgcga aggcccgtga gcgggtccgt gatcgccgcg
     2761 tcctgcgccg tctggtggag ccgcgcattc gcaagagcca ggccaacctt gccggcgaga
     2821 aggccggcga tctccttctc ctgggtggtg aacggtgtgc cgagatccct gcgcagccag
     2881 gtcaccacgc cggccacctc gtcgccgatc accatcgggg ccgaaatcgc agcgagcaca
     2941 tcggcaagct ggaggcctgc gactgccctg gagtaacgag cgcgctcgag tcgatcttcc
     3001 accaccacgg cgcgagtgga aatcgctcga ccggtgatgc cctcacctgg ctcgagagcc
     3061 tgtccgatca ccatcgtgtc gccgccggcg accgcgttga ccacgtaggc gccagtcgcg
     3121 tcgtcgcgat tcacgaggag gagcgcatcg gcggggatga cgcgcagcgc gccgatcgcg
     3181 acctcttcat ccatcgtcgc cggatcgagg ctcgagccga ggacggtcgc gaatgtcgtg
     3241 agtcgatcga gcagcgccgc gcgctccgtg agcttctggc gctcgcggcc gagggccagc
     3301 gcagcggcga ggcgatcacc gacgatctgc atgagcccga agtcctcgtc gcggagccgc
     3361 cggtcaccgc tggtctcgac gttcaggatg ccgaggagct cgccgtcgcc catgaggggc
     3421 acgccgatct cgctgacaac attggaatcg gccgagagga agtcggggtc ggaccgtgcg
     3481 tcgggcagga acacggcctc gcgcgagtgg tagatccgcc cgaggatgcc gcgatccggg
     3541 ctgatggtct ggatcggaga ctgatagttg cgctgcgctc cgagctggag cgcggtgccg
     3601 tcccagacgt acaccgacgg gtagtggtag ccgaaggtct cctcgagcac cttcatgacc
     3661 cggtcgagcg ccgcttttgt gggcccttcg cgcgcgagga cgctgccgac cgcctcgacc
     3721 gccgcgagct gccgcgcgtg gcgccgggcg gccaggttcg cgcgccggag gcggttgctc
     3781 gagcgctcga gcttggcgac gatctgacga gtgccgtacg acagcacgat tgcggccatt
     3841 gcgacggcaa agacctcctg gcggtccgtg acgtccaggc cgggcaggcc aaggggcacg
     3901 agcatgagga ccatgccggc cgctgcgacg agcaccccgg cgcgcccgcc gccggcggcc
     3961 gcgagggcca caaccgggat ggcgagggcg ctcagcggcc aggtgccgct tggatcaatc
     4021 cagagattgg cgagcccgat gaaggcgagg cacagcgcga gccgggcggt ccccagccat
     4081 tcgggcggct tgcgcaacgt cgtcagccac agctgtgcgg ccacgaagat cagtggcgcg
     4141 aggagcccca tcgggcgctg ggaaagatcg gcgatgccct ccatcgcgac gaccgggagc
     4201 gcgatcgcga tcgcgaggag caggtacgtg tcgctgcgaa tccgcgccgc cagccggctc
     4261 cgcgtccagc ccgaaccagc cgccgagtcg ccggcggcgg agaggaaccg ctcgagcggc
     4321 cggtaggtcg gtcgcgcgat cggggaaggt ggggtcggcg gacgatccac gctggccttc
     4381 ggtgtgcagg cacggctcgg cccagtcaac ggctggacac cgcggatcgt gcggcggcgg
     4441 tgcaccctgc caccatcccc cgtccgtacc ccctcgttgt cagttcggga ggacgaagac
     4501 ctcaccctgg tcgttggcga agatcgaggc gtagccggcg gcgatcagct gttcgcgggt
     4561 gccctcgggg acgccgtcgg gctcgagcac gacatacggg aagaccggtg ctgccaggta
     4621 ggaaggcatc gcctcgaacc cctcgagacg gcctccgacg gtctggtgcg tcacctcggg
     4681 gtcgaggctg atgaaggtct cggtgacgcc gtcgaacacg ccgagcggcg tcgcgaccca
     4741 ttgctggaag ctcttcgcga tgtgcagcac tggggtgtcg tgctggaggc gaccagcatc
     4801 gatctcggcg cgcaccgcat cgaggatcgc ccggcggggc tcgtcgacga tgtggcggga
     4861 gtcggggaag ccaacccagt agccactgtc cgcgtacttg aggtcgatcg cgaacgtctc
     4921 cgaccagcga tgctcgccga gatggcggga gtcgatgggc tcgggccgca ccgggagcgc
     4981 ggcgatcgcg acgaatgcgg cgaggccggc ggcacggacg agccagggca atcgatcggt
     5041 ggcccagagg taggcgaggg ctgcggcggc gcccgcggcg gcgatcgacg acagccagta
     5101 gtggacgctc ttgggcacct cgaaccgcag ggcatcgccg aggaagccca gatcgtccgg
     5161 cagcacgttc gcgagcaaca cggcgaccac gccgaccagt gcgcccgccc agacgagtgg
     5221 tgatggggca ccggtgcttc ccgccagcgc gccggcgatg aggacgacaa gcagcaggcc
     5281 gctcgattcc aggaccagcg tgagcgtggg gtcgaaggcc ttcggcacac gctcgacgtc
     5341 caggatcgag gcggcgaaag cgatcgcgat gccaccgacg accacgacga cggcgacgaa
     5401 ccggccgatc tcggcggctc ggacgatccc atcgcggagc ctttccctcc tcgcgatcag
     5461 ccgacctgcg gcgagtccga ttgccacggc aacgggcagc cagacgccca gggcgagcgt
     5521 cggcaggctg gcgccgagca tggtcgcgag ctgcggcagc gcgatcagtc ccgcggtgag
     5581 ggtcgcgacg aacgcatccg cgccgaggtc gcgcggccgg gcgaggcccg cgatcccgac
     5641 ggtcacgatc gcgaagaacg cgatcacggg gtggacgagc gtcgccgctc cgagggcgag
     5701 gccgatgacc gctccccgcc cgatccgcca cgggtcttcc atgcggcgga gcgagtcggc
     5761 ggcaagcgcc agcccgaggc agatgagggg caccacgaca acggtgccgc gggcgtcggc
     5821 aaggcgggcg aatgactggc tcagcgcgaa cgccaggagt gcccaagcgc cgatcgggac
     5881 gtcggttcga tcgcggagcg cgctcgccag ccggtacgcc ccggcggcaa cgaggacggt
     5941 ctggggcgcg atgaacgcgg cgatggcgag gccgccaggg aggttcgtca tcgtcgcgag
     6001 tgcgccaagc agcccgtcat agccgagcac ggttcgcgag ggtccgatga tcggcgactg
     6061 tgtctcgctc agcggcgaga accagccgaa ggtgcgcagg tgctcgaccg gcccgacgta
     6121 gttcggcagc acgtcgatga acgggacaac cgggctggcg agctgcagga cgaccggccc
     6181 gatccagacg atgccgacga gcgcggcgac gggccacgac ccgatcgagt ccagcgcatc
     6241 gcgccagctc gcgaacgcgg tcagcggtcg gacgtggcgc gagatcggcg cgaggagcag
     6301 cgcgccggca atgatcgcga catcgatccc gatcagcacc ggggcgcgga acaggccgaa
     6361 cccggcgacc gttccgagca gcagcgtgtc cttgagcacc gcggcaacca tcgcggcgag
     6421 gagggattcg gaaaccgagt cgaacgctcg tccacgggcg gcgcgcgcga gcacgaggcc
     6481 ggtcgcgagc tcgagggagg tcgcagccag gatcaggagc gcgccgggga gggtgacgga
     6541 gagctggctc aggactccgt tgagtgcctc aacggcgtcg aacccgagct tctcggacgt
     6601 gtcgagcatg accagcccga gcaggaacgc cgacagcccg atgaccccga ccaggtccag
     6661 ggcgatcgcg tcacgatccc gaagctgccg accctccacg gcgcggatgg tacgggcgcg
     6721 gcgtcacgcg tcgtcgggtc gacgaaggga attggagcgg gcggcggggc tcgaacccgc
     6781 gacattcaga ttggaaatct gacgctccac cagctgagct acgcccgcgc ggcaaagatc
     6841 gtaccgcatc atctgcgcgg tacagacgta ccgcggcgac tcggtgaaga tggggcatga
     6901 tggcgcaatg acggggcgtc gcatctcgag actcgggctc gcggcgttgc tcgccgggct
     6961 cgtcgtcggg ccgaccgcgc agcatgtcgc ggcggccgac ctcgtcctgc ccgacctggc
     7021 aatgatggcg ccgttcgact tccgcatcga gatctcgccg tcggggcgga agctgctccg
     7081 cttctcgacc gtcgccgtca acatcggcaa aggcccgttc cagctctacg gtcacgacgc
     7141 cgacggcgcg aagatcggcg acaccctgca gatcacccag ctgatcaagc gcaccgacgg
     7201 gacgtatatg ccccgcgact cgaccgcgac gatgacctgg tcgggcgatg gtcacaacca
     7261 ctggcacatc aacggctacc agcgattcgt gctcaacaag ctcgacggga cccacctgcg
     7321 ctacgtcaag aagaccgggt tctgcgcgtt cgacagctac cggtacggct cggcgctgcc
     7381 ggccttctac acgtgggacg cgtacgcgtg tcggacggcc ccgaacggcg aggtcccgat
     7441 gggcacgtcc cgcatgtggg gcgacatcta caagtcgacc atcgcgttcc agtggatcga
     7501 catcacgggc ctcgcgagcg ggacctaccg actgaacatc tcggtcgatt caccgcgcac
     7561 gaccggcggc aggttcctcg aatcgaacga gtcgaacaac ctcgcctggg ccaagatctc
     7621 gatcggcaaa acgaccgtca cggtcatctc gaagagcgcc aagccctagc caaccggcgg
     7681 cgatccgcca ctcccgccga gcaggggtac gatggcgagc cgatgaccgt cgaatcgccg
     7741 ccgcggagct gcccgcgctg cgaggccgcc gttgcggccg atgcgcgctt ctgtgccagt
     7801 tgcgggcagg ccctcgccga atccaccagc ggtgacgagg ccctccacac ccgcctgacc
     7861 gcgaccgcgc ccgagccgct catccagaag atgcgcgccg ccaagctcac cggcgagcgc
     7921 aagcccgtga ccgcgctctt cgccgacgtc gtcggctcga cgacactcgc cgagcagatc
     7981 gaccccgagg actggaccgc gatcatcaat gaggcgttcg acctcatgtc gcgtgccgtc
     8041 ttccgctacg agggcaccat cgcccagctc cagggcgacg cgatgctggc gttcttcggc
     8101 gccccgatcg cccatgagga cgacccggag cgggcgatct tcgccgcgat cgacatgctc
     8161 gccgcgatcg aggagttcgc ggcacagctg cgaacgtccc acggcatcga gttccggatc
     8221 cgggccggga tcaacagcgg cccggtcatg gtcgggaaca tcggctcgga cctgcgctac
     8281 gagtacacgg cgctgggcga tgccgtgaac gtggcggccc ggatgcagtc gtcggcacag
     8341 ccggggacga tcgtcatcac cgagatgacc cgccggctca ccggcgacac gttcgaggtc
     8401 gatgacctcg gcgagatcga cgtcaagggc aagaccgaca agatccacgc cttccgcgtc
     8461 gtcggtcgaa agtcggcgcc ggcgcggcgc cgcggcctgg agtcggtcgg gctcgacagc
     8521 ccgatggtcg ggcgcgaagc ccagctcggg cggctggtcg gcctgctcga cgtcatccgc
     8581 tcgggccggg gtcgcgccgc attcgtgatc ggcgagccgg gcatcggcaa gagccgcctc
     8641 ctcgccgagt tcaagcgcat cacgctcgcc acggacgcgg cgccgacgag gtggatcgag
     8701 ggtcgctgcc tgtcgtacgg ccggggcctc ccttatcacc tgctcatcga cctgctccgg
     8761 tccatcctcg acgtcccgtt ctcggcctcc gagacggaag cgcgggccgc catcgaccgg
     8821 acgctcgaac ggctcctcgg cgccgatccc gccgaggtcg cggacacggc gccatacctc
     8881 gcccacctgc ttgacctgcc gctcctgccg ggcgaggaga gcgtgaccaa ggtggttccg
     8941 gacgtgctcc agagccgcta catcgcggcc gtgcaccggc tccttcgcag catcgccgcg
     9001 gagcgaccgc tcgtcctcgt ctgcgaggac atccactggg cggatccggc atcggtcgcc
     9061 gtcgctggcc agctgatgcc cctcgcctcg cagctgccga tcctgttcgt gggcgcgatg
     9121 cgggccgaga tggactcggc cggctggctg ctgctgcagc aggcacgcga cctgttcggc
     9181 aacgacctca ccgagatcca gctcgagccg ctgaccgagg ccgagggccg cgccctcgtc
     9241 tcgaacctgc tcgagatcga atcgctgccc gagcgaaccc gcgacctgat catggctcgc
     9301 gcggagggca acccgttctt cgtcgaggag gtcgtgcgca tgctcatcga gcgcggcgtc
     9361 atcgtcgccc agggcgaccg ctgggtggcc accgacgagg cgggtggcgt cgagatcccc
     9421 gagagcctgc acggcctgct cctggcccgc atcgaccagc tgcccgacgc aacgaagcgt
     9481 tcactccgga tcgcggcggt gatcggccga caattccccg tgcgcgtcct cgagcgcgtc
     9541 gtcgcagagt cagtcgtgac gccatgacca ccgagaagca cgcggacacc gacctcgaca
     9601 ccgacctcgc gaccctcacc tcgaaggggc tcatcaagcg cgccgccgag ctccccgagc
     9661 tcgagtacct gttccgccac gcgctcgtgc aggacgcggc ctacggctcg ctgctcaagc
     9721 aggagcggcg agagctccac ggtctcgtcg gcgaggccct cgaggagctc tacccggata
     9781 ggaccggtga gctggcgccg gtgctcgcga tgcacttcga gcaggcgggc gagacggaca
     9841 aggccatcga ctacttcgcc gccgggggtc agcacgcgct ccagcagaac gcgatccagg
     9901 aggcctacgg tgcgttcgac caggcatccg ggctccttgc tcgaaccccc gcgcctgccc
     9961 cgggtgctcc cgccgagtcg ccgcttcggc aacgacgtcg catcgagatc aagctgggtc
    10021 gggcgcaagc cgggtacagc ttcctctcgc cggaccaggc gttcgactcg gtcgaggaga
    10081 tcgtggccga ggccgaagag ctgggcgacc tcgagctcct ggcgcgcgtc catgccctca
    10141 tcgcactcgg gctgatgcag tccggcgtcc aaccgaccga gcccagggtg gcgcggtcgc
    10201 tggcgcggat cgaggagatc ggcatcgcgc tcggcgaccc gggcatccgg gcgatgccgc
    10261 tcgcctttgt cgggctcagc caggtgttcg ccgggccggt gcgagagggc gtcgcggcgc
    10321 tcgagaaggc ggtcccgctg atgcaggacc agaaggactc gatcggcgca gcattcgccc
    10381 gcggcggcct ggcgatgggc tatgcgacgc tcggcgaatt cgacaaggcg gatgccgccg
    10441 ccgcaaatgc gaaggagctt gcgaaagagg gcgacctgat cgcgcagctc gacgcactca
    10501 tcgccgagtc aatggttcgt gcgcagcagg gacgtctcga cctggctgtt ccgctggccc
    10561 gagaatgcgt tgaccgggcg gaatcgaccg gtgcctcggc gtgcgtcctc gcctcgtcgt
    10621 ggatcctcgg cgacgcgctc catcggctgg gcaagttcgc cgacgcgcgc gacgtcctcc
    10681 ggcgcggcgc cgatgtcgcg ctgatggtcg accgctacgt ctggcggccg acgctccagg
    10741 cgtggctgcg cagcgccgca gcggccctcg gcgaagtcga ggagggcgac gtcgatgagg
    10801 cgctcgccac cgcccggtcg atcggcaatg agctcggcga ggcgggcatc ctcggcaaac
    10861 gggccgaggc agccgcgagc aggtcggagt tcgactcggc gctggccgat tacgccgcga
    10921 cggcggcgat cttcgaggcc cagggcgcgc gcccgtcgct cgctcgggtc ctgcagggct
    10981 ggggcgagac tcttcgccga gccggccgcg acgccgaggc aacggcgacg ctcgaacggg
    11041 ccgccgcgct gttcgacgag ctcggcctca cccgcgaggc aggcgccgtt cggacctcga
    11101 tcacgttggg cagcaccaag ctcgcgttca actgacgccg cgagctcgac ccaaacgaca
    11161 ccaaggaggc cggccctctc ggaccggcct cgctcgatgc aggactttgg gtggctcagg
    11221 ttcggcggag gttcaggcct cgcgtcgtgc ccggaacgcc ggtggtcgag aaggcgtagt
    11281 tgaccgtcgc gggcgacggc ggggtcgcgc ccttgaccgt gcgcgtgctg agcttgacgt
    11341 tgatgtagta cgtgcccccg gtgggcgccg tgaaggtcac gctgatcgtc ccagcggcgg
    11401 cattggtcga tgactggctg aaggttgcgt tcgggccgcc cgtgcagttg gaccggaaca
    11461 cgccgctgcc gttgccgttg gcgagcccga acagggtcga gaagttgccg ctgaggatcg
    11521 actggttgac cgtgaacgtg ttgctgcccg cgaccgcggt cacgttgacc cagtacgaga
    11581 acgagctctc gctgagcgtg ttgatcttgg tcttgttcgt gctgtaggta atctcggtca
    11641 acgtcgtggc cgtggcgttc gcgaaggtgg cacagctggc gtttgccgag agctgggcgg
    11701 tgacgaccgg caggttggtc agatggaagg tcgccgacgc cgtgccggac gaggccacga
    11761 agtcgtacgg cccacccggt gtcccatttg cacgtgcggt gacgaccagg ttgccgttcg
    11821 agtccgtgat gtacgggccc gtctcgacga tcgacgccga cgcgcccgat agcggtcccc
    11881 cgaaggtgac cgtggcgttc ggcacgggat tgccgcccgc gtcgagcacg gtcaggtcga
    11941 gccgggtcgg gaagttcgtg ttgatcgtcg cgagctggtt gtcgcccgcg ttgatcgtga
    12001 tgctggcggc cgcgccgacc aggttcgtca ggtggaaggt ggcggtgccg ctcccggacg
    12061 tcgcgacgag gtcatacggc ccgccggcga tcgagttggc gtgtgcggtc acgacgaggt
    12121 tgccgtcgct gtcggtgagg tacggcccgg gctcgacgat cgacgccgag gcgcccgaga
    12181 gcggcccggc gaaggtgacc gccgcgcctg ccaccgggtt gccctcggag tcggtgacgg
    12241 tcaggtcgag ctgcgtcccg aaatccgtat tcacggttgt gctctggtcg tcgccgtcg
//
LOCUS       DATBSW010000137         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_998434, whole genome shotgun sequence.
ACCESSION   DATBSW010000137 DATBSW010000000
VERSION     DATBSW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998434"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VIF63_03820"
     CDS             <1..390
                     /locus_tag="VIF63_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238541.1"
                     /translation="HVDVSRGDMLCRPHNIPTIAQEIDAQVCWMDEGASMTEGAKYSI
                     KHTTRWAKALVREIAYSIDVNTLHRNEGVQGLALNEIGRVRLRVTQPLFVDPYSENRQ
                     TGSFILVDERSNKTVAAGVIGRATVAS"
     gene            complement(422..1066)
                     /locus_tag="VIF63_03825"
     CDS             complement(422..1066)
                     /locus_tag="VIF63_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238542.1"
                     /translation="MSDQRSSPEHVSEPDLEPEAPTTPIAPMTGVATGSYGTASNLED
                     TQVYLSGIPIDDPPPGDLPPPAEVPDQEPPPMQFPGDVPIEVPGDTPPPMDAPRRDPV
                     PIDVAAVASAAPRRRGSAAPASSAARRRPVAWAGLAAAAVVAVLVGVILGSSGNGPGA
                     ANGIGAGASVGVAATPAPTDEGDGKGKDGKGNGNGNGNGNGNGNGKGKGNGNGN"
     gene            complement(1152..2243)
                     /locus_tag="VIF63_03830"
     CDS             complement(1152..2243)
                     /locus_tag="VIF63_03830"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8238543.1"
                     /translation="MQLPIERARQIVVMAQRLDAGRPAGILEAVRELGFLQIDPTAAV
                     ARTEHLVLWARLGRTFDPADLARLLVERKLFEHRAFIYPIEDYPILRAEMDVWPDREW
                     SFHKRIRAWLATNADFRAHVLEEIRKRGPLRSRDLEDESVADWRSRGWTHQRNVTQML
                     DYLSGQGAIAIAGRAGNERIWDLAERVLPTDSPRISREEANAIQAQRRLRTLGAVRVG
                     SPYDVGDIGTEVEIEGVRGRWRVESELIDRPFVGRTALVSPFDRLVYDRQVTEALFGF
                     EHRLEIYVPAPKRRWGYYVLPALHGDRLVGRVDARADHAAGVLRVPALHLQSDATDDD
                     QAAIRAELDELAAWLRLPKVEVEQVVRGA"
     gene            2365..3564
                     /gene="carA"
                     /locus_tag="VIF63_03835"
     CDS             2365..3564
                     /gene="carA"
                     /locus_tag="VIF63_03835"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY8238544.1"
                     /translation="MAAFRYAAPPTSPIRPAPPSPRASAIVDPRIGDLGPALLALEDG
                     TIFDGVAFGAPVSGGGDLVVNTSQTGYQEVCTDPSYAGQVVVMTYPLIGNYGRLVADD
                     QSARPWLRGLIVANATAAVLDDARQLARLLRDNGIPAIAGVDTRALARHLRANGSLRG
                     AILEPGALDRAAAARRAAAVPRWEDQDFVAEVSPAAVTEIGAGEDGPLIAIVDYGLKA
                     NIVRSLRRRGVRVRVLPHTATEAEVLAADVSGVVLSPGPGDPARLAGPVALARAIIAD
                     GRPLLGICLGHQIVGRAAGAETRRLRFGHHGANHPVRDIDTGFVQVTAQNHEVQVVGD
                     SLPASSGFHVSQVNLNDGSVEGLRHVEKPIETVQYHPEGAPGPLDALAVFDRFVAACG
                     ATPVAGA"
     gene            3576..4088
                     /locus_tag="VIF63_03840"
     CDS             3576..4088
                     /locus_tag="VIF63_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238545.1"
                     /translation="MIGSGGAGKSTFARDLGARTGLPVIHLDGLFWGPNWQPMPKPEW
                     IELERRLIADERWIMDGNYGSTQDLRFPRADTVVFLDLPRLVCIWSVVRRWSTYRRHG
                     RPDMPQNHDKLELTFLRWIWTYPNEKRPEVLARLEKLPATTTVVRLRSRREIREYLAG
                     VAPAGEAAAA"
     gene            4085..>5213
                     /gene="carB"
                     /locus_tag="VIF63_03845"
     CDS             4085..>5213
                     /gene="carB"
                     /locus_tag="VIF63_03845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY8238546.1"
                     /translation="MTTKPASVLIIGSGPVVIGQAAEFDYAGTQACRALRAEGVRTIL
                     VNSNPATIMTDPDVADAVFLEPLTVDAIEAVIAREKPEGLLAGLGGQTALNMAMDLAR
                     AGVLQRHGVKLLGTPLEAIRMAEDREAFRDLLDRIGQPYAPSSIIEGDTVAAREASAN
                     HALEVIGLPAIIRPAFTLGGTGGGIVETEPAYRERVRAGLRASPIGQVMIEKCLVGWQ
                     EIEYEVMRDADDTCIAVCSMENVDPLGVHTGDSIVVAPVQTLPDAVHQRLRSAALAII
                     RALGVEGGCNVQFALSPDSTEYAVIEVNPRVSRSSALASKATGYPIARVAAQIAVGRR
                     LAEIPNVVTGTTVAAFEPALDYVVVKLPRFPFDKFPGADRSL"
BASE COUNT          740 a         1881 c         1833 g          759 t
ORIGIN      
        1 cacgtcgacg tgagtcgggg cgacatgctg tgccggccgc acaacatccc gacgatcgcc
       61 caggagatcg acgcccaggt gtgctggatg gacgagggcg cctcgatgac ggagggcgcc
      121 aagtactcga tcaagcacac gacccgctgg gcaaaggcgc ttgtgcgcga gatcgcctac
      181 agcatcgatg tgaacacgct gcaccgcaac gagggcgtcc aagggctggc gctcaacgag
      241 atcgggcggg tccggctgcg cgtcacgcag ccgctgttcg tggatcccta ctcggaaaac
      301 cggcagaccg ggtcgttcat cctggtcgac gagcgatcga acaagaccgt cgccgcgggg
      361 gtcatcgggc gggccaccgt cgcgagctga tccctcggtt tcgcgtcgct acgcccgggc
      421 gctagttgcc gttcccgttg cccttgccct tgccattccc gttgccattg ccattcccgt
      481 tgccattgcc attgcccttg ccgtccttgc ccttgccgtc accttcgtcg gttggagcgg
      541 gcgtcgcggc cacgccgact gaggcgccgg cgccgatccc attggcggct cccggcccgt
      601 tgccggaaga tcccaggatc accccgacga gcactgcgac caccgccgct gcggccagcc
      661 ccgcccaggc aacgggacga cgtcgggcgg cggacgacgc aggcgcggca gaaccgcgtc
      721 ggcgaggcgc ggccgatgcg acggccgcca cgtcgatcgg cacgggatcg cgacgcgggg
      781 cgtccatcgg cggcggggtg tcgccgggga cttcgattgg gacatcgccg gggaactgca
      841 tcggcggtgg ttcctggtcg ggcacctcgg ccggcggcgg caggtcgcct ggcggcggat
      901 cgtcgatcgg aatcccgctc aggtacacct gcgtgtcttc gaggttgctt gccgttccgt
      961 agctgcccgt ggcgacgccc gtcatcggcg cgatcggcgt tgtcggggcc tcgggctcga
     1021 ggtcgggctc ggagacgtgt tcgggtgaag accgctggtc ggacatgctc gcagcgtaga
     1081 ccctgagcgc gcaatttccg gtgcgggccc tcgatcggat ggtcgtacag ccccgttaca
     1141 accgcggatg ctcaggcgcc gcgaacgacc tgttcgacct cgaccttcgg cagtcgcagc
     1201 catgccgcca gctcgtcgag ctcggcgcgg atggccgctt gatcgtcgtc ggtggcgtcc
     1261 gattgcaggt ggagcgccgg tacccgcagc acgccggccg cgtggtccgc ccgggcgtcg
     1321 acgcgcccga ccagtcgatc gccgtgcagc gccggcagca cgtagtagcc ccagcgtcgc
     1381 ttcggcgcgg gcacgtagat ctcgagccgg tgctcgaacc cgaacagcgc ttccgtcacc
     1441 tgccggtcgt acacgagccg gtcgaacggc gagacaagcg cggttcgccc gacgaacggc
     1501 cgatcgatca gctcggattc gactcgccag cgcccgcgca cgccctcgat ctcgacctcc
     1561 gtgccgatgt cgccaacgtc gtacggcgat ccgacacgca cggcgccgag cgtgcgcagc
     1621 cggcgctgtg cctggatcgc attggcctcc tctcgcgaga ttcgcgggga atcggtcggc
     1681 aggacacgct cggcaaggtc ccagatccgc tcgttgccgg cgcggccggc gatcgcaatc
     1741 gcgccctgcc cggacaggta gtcgagcatc tgggtcacgt tccgctggtg cgtccagccg
     1801 cgtgatcgcc agtccgcgac ggactcgtcc tcgaggtcgc gcgagcgaag cggcccgcgc
     1861 ttccggatct cctcgaggac gtgcgcccgg aagtccgcgt tggtcgccag ccacgcccgg
     1921 atgcgcttgt ggaacgacca ctcgcggtcg ggccagacat ccatctccgc gcgcaggatc
     1981 gggtagtcct cgatcgggta gatgaacgcc cgatgctcga agagcttgcg ctcgaccagg
     2041 agccgggcga ggtcggccgg gtcgaacgtc cgaccgaggc gcgcccacag cacgaggtgc
     2101 tcggtgcgcg cgacggcggc ggtcggatcg atctggagga agccgagctc gcggaccgcc
     2161 tcgaggatcc cggcgggccg cccggcatca agccgctggg ccatcacgac gatctggcgg
     2221 gcgcgctcga tgggcagttg caccaccgga cggtagcggg tcgagcgtcg tcgcgagggc
     2281 gcgcctgcta cccttcgggt gtccttgacg gccggtcctg tgaggccggc gaaccggttc
     2341 cgtgaggccg gaaaggagtg gtgcatggcc gcgttccgtt acgccgcgcc gcccacttcc
     2401 ccgattcgac ccgctcctcc ttcgccgcgt gcgtccgcga tcgtggaccc gcgaattggc
     2461 gacctcgggc cggccctgct cgcgctcgaa gacggcacga tcttcgacgg cgtcgcgttc
     2521 ggcgcgcccg tcagcggtgg cggcgacctc gtcgtgaaca cgagccagac cggctaccag
     2581 gaggtctgca ccgacccgtc atacgccggc caggtcgtcg tcatgacgta cccgctgatc
     2641 ggcaactatg ggcggctcgt cgcggacgac cagtcggcgc ggccatggct gcgcggcctc
     2701 atcgtggcga acgcgaccgc tgcggtcctc gacgacgccc gccagctcgc gcgcctcctt
     2761 cgcgacaacg gcatcccggc gatcgcgggc gtcgacacgc gggcgctcgc caggcacctg
     2821 cgtgcgaacg gcagcctccg cggcgccatc ctcgaacccg gcgcactgga tcgcgccgcc
     2881 gccgcccggc gggccgcggc cgtgccccgc tgggaggacc aggatttcgt cgccgaggtc
     2941 tcgccggcgg cggtgaccga gatcggagcc ggggaggacg gaccgctgat cgcgatcgtc
     3001 gactacgggc tgaaggcgaa catcgtccgc agcctccgcc ggcgaggcgt ccgggtccgg
     3061 gtcctgccgc acaccgccac ggaggcggag gtgctggccg cggacgtctc gggcgtcgtc
     3121 ctctcgcccg gccccggcga cccggcgcgg ctcgccggcc ccgtggcgct cgcccgggcg
     3181 atcatcgccg acggccggcc gctgctcggg atctgcctcg gccaccagat cgtcggtcgt
     3241 gcggctggcg cggagacccg gcgactgcgc ttcgggcacc acggcgcaaa ccacccagtc
     3301 cgggacatcg acaccgggtt cgtgcaggtg accgcccaga accacgaggt ccaggtggtc
     3361 ggcgactccc ttccggcctc gagcgggttc cacgtgagcc aggtcaacct gaacgatggc
     3421 tcggtcgagg ggctccgcca cgtcgagaag ccgatcgaga ccgtccagta ccaccccgag
     3481 ggcgcgccgg gtccgctcga cgccctcgcg gtcttcgacc gtttcgtcgc cgcctgcggc
     3541 gccacgcccg tcgccggcgc gtgaatcggg ttgcggtgat cgggagcggc ggggcaggga
     3601 agtcgacctt cgcccgcgac ctcggggccc ggaccggctt gccggtgatc cacctcgacg
     3661 ggctgttctg gggcccgaac tggcaaccga tgccgaagcc ggagtggatc gagctcgagc
     3721 gccgcctcat cgccgacgag cgctggatca tggacggcaa ctacggttcg acccaggacc
     3781 tgcggttccc acgcgccgac acggtcgtct tcctcgatct gccgcgactc gtctgcatct
     3841 ggagcgtggt ccggcggtgg tccacgtacc ggcgtcacgg gcggccggac atgccccaga
     3901 accacgacaa gctcgagctc acattcctgc gctggatctg gacctacccg aacgagaagc
     3961 gaccggaagt cctcgcccgc ctcgagaagc ttccggcgac caccaccgtc gttcgcctgc
     4021 ggagccgtcg agagatccgc gagtacctgg ccggcgtcgc gccggccggc gaggcggccg
     4081 cagcgtgacc acgaaacccg cgtccgtcct catcatcggc tccgggccgg tggtcatcgg
     4141 ccaggcggcc gagttcgact acgccggcac ccaggcgtgt cgcgcgctgc gcgccgaggg
     4201 cgtgcgcacg atcctcgtca acagcaaccc ggcgacgatc atgaccgacc ccgacgtcgc
     4261 cgacgccgtg ttcctcgagc cgctcaccgt cgatgcgatc gaggcggtca tcgcgcgcga
     4321 gaagcccgag ggcctcctcg ccgggctcgg cggccagacg gcgctcaaca tggcgatgga
     4381 cctcgcgcgg gcaggcgtgc tgcagcggca cggcgtgaag ctcctgggca cgccgctcga
     4441 ggcgatccgg atggccgagg accgcgaggc attccgcgac ctgctcgacc ggatcggcca
     4501 gccgtacgcg ccgtcctcga tcatcgaggg cgacaccgtg gcggcgcggg aggcatcagc
     4561 gaaccacgcg ctcgaggtga tcgggctgcc ggcgatcatc cggcccgcgt tcaccctcgg
     4621 cgggaccggc ggcggcatcg tcgagaccga gcccgcgtac cgggagcggg tacgggcggg
     4681 cctgcgtgcc agccccatcg gccaggtgat gatcgagaag tgcctcgtcg gctggcagga
     4741 gatcgagtac gaggtcatgc gcgacgcgga cgacacgtgc atcgcggtgt gctcgatgga
     4801 gaacgtcgac ccgctcggcg tccacaccgg cgactcgatc gtcgtcgcgc ccgtccagac
     4861 cctgccggat gccgtgcacc agcgactgcg gagcgccgcc ctggcgatca tccgggcact
     4921 cggcgtcgag ggcggctgca acgtccagtt cgccctctcg ccggactcca cggagtacgc
     4981 ggtcatcgag gtcaaccctc gcgtgtcacg aagctccgcg ctcgcctcga aggccactgg
     5041 ctacccgatc gcgcgggtcg ccgcgcagat cgcggtcggg cgtcggctgg cggagatccc
     5101 caacgtggtg accgggacca cggtggccgc gttcgagccg gccctcgact acgtggtggt
     5161 caagctgccg cgcttcccgt tcgacaagtt cccgggggcg gaccgctcgc tcg
//
LOCUS       DATBSW010000138         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_646248, whole genome shotgun sequence.
ACCESSION   DATBSW010000138 DATBSW010000000
VERSION     DATBSW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646248"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VIF63_03850"
     CDS             <1..394
                     /locus_tag="VIF63_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8238547.1"
                     /translation="QEVAASLAARTHLVLLCPRYEGIDDRIRDLVDLELSIGDYVLTG
                     GELAALVVIDAVTRLLPGAIDDASTIEESFSAGLLEYPQYTRPAEFRGQGVPDVLVSG
                     NHEAVRRWRHDAAVARTRARRPDLLPPE"
     gene            505..855
                     /gene="rplS"
                     /locus_tag="VIF63_03855"
     CDS             505..855
                     /gene="rplS"
                     /locus_tag="VIF63_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8238548.1"
                     /translation="MNVLDEIVADQIRTDLPDLASGDTVKVSAKVVEGGKERIQVFEG
                     TIMKLKGGGIARSITVRKIASGVGVERTFMVNSPRVEKIEVVRHGRVRRAQLYFLRDR
                     VGKAATLRERRTRS"
     gene            929..1915
                     /locus_tag="VIF63_03860"
     CDS             929..1915
                     /locus_tag="VIF63_03860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY8238549.1"
                     /translation="MAPRVPTLRFERSHWRDGAERVVGIDEVGVAPTCGAVVAAAVIM
                     RPGAHRIPGVRDSKTLSAAQRERLAPIIRRRAVAIGLGAASVREIDAINIYHATHLAM
                     RRAVGRLGGHDHVLVDGNRIAGFEAAVGPYTAIVDGDAKCYTIACASVVAKVVRDRMM
                     TLLSARYPGYGWERNQGYATQEHRDAIRRLGLTPFHRRSFLALQRTLAGDQISLDLFD
                     EAVASVEQEADVRDLDRLDGSPGLAEAPDIAVVSGFGDVTGFADVPGFGEVPGPGEVP
                     SLDELAELSGLSAADLVVTAPDPSAAAAIAEAEALVRELADSPAKAGLDSGA"
     gene            2005..2379
                     /locus_tag="VIF63_03865"
     CDS             2005..2379
                     /locus_tag="VIF63_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY8238550.1"
                     /translation="MRTAAQQSGDAAEARVAGRLEQLGWQILATHVRVGRAELDLVTI
                     DPGPPATLVIVEVRWRGRRDFGLPEETVDGRKRARLHRAGFALREQGRLPDGRPLPPL
                     PLRFDLVVLEPGDRMRHHRHAA"
     gene            2797..>3037
                     /gene="rpsB"
                     /locus_tag="VIF63_03870"
     CDS             2797..>3037
                     /gene="rpsB"
                     /locus_tag="VIF63_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8238551.1"
                     /translation="MPTISMRQLLEAGVHFGHQTRRWNPKMKPFIFAERNGIHIIDLA
                     QTVQRLDVALDHVRETVARGDQVLFVGTKKQAQEPV"
BASE COUNT          468 a         1085 c         1056 g          428 t
ORIGIN      
        1 ccaggaggtc gcggcatcgc tggcggcgcg cacgcatctg gtcctgctct gcccgcgcta
       61 cgagggcatc gacgaccgga ttcgcgacct cgtcgacctc gagctgtcca tcggtgacta
      121 cgtgctgacc ggcggcgagc tcgcggcact cgtggtgatc gatgccgtga cgcggctcct
      181 gcccggggcg atcgacgacg cgtcgacgat cgaggaatcg ttcagcgccg ggctcctcga
      241 gtacccgcag tacacgcgac ccgccgagtt ccgcggccag ggcgtgcccg acgtcctcgt
      301 cagcggcaac catgaggcgg tgcgccgctg gcgccacgat gcggcggttg cccgcacgcg
      361 cgcccgccgt cccgacctgc tcccgccgga gtagccgggt cgctgcggcc tcgtggtatg
      421 ctccgcgacc gtcggccgac agccgatctc caatcgaatt ccagcggcgc tcacgcgcgc
      481 ctcgaccagc agggaaccac caccgtgaac gtgctcgacg aaatcgtcgc ggaccagatc
      541 cgtacagacc tcccggacct cgcctcgggc gacaccgtca aggtgtcggc caaggtcgtc
      601 gagggcggca aggagcggat ccaggtcttc gaaggcacga tcatgaagct caagggcggc
      661 gggatcgcgc gctcgatcac ggtgcgcaag atcgccagcg gcgtcggcgt cgagcgaacg
      721 ttcatggtca acagcccgcg cgtcgagaag atcgaggtcg tgcgacacgg ccgcgtccgg
      781 cgagcccagc tgtacttcct gcgcgatcgc gtcggcaagg cagcaacgct gcgcgagcgc
      841 cgaacccgga gctagccgct ccagccggtc gcgtccggcc tcccccggca gccggcggag
      901 cgtcgccggc ggtaccctga ccgggaccat ggcgccccga gtccccaccc tccgtttcga
      961 gcgcagccac tggcgtgatg gcgccgagcg cgtcgtcggc atcgacgagg tcggcgtcgc
     1021 cccgacgtgt ggcgcggtcg tcgcggccgc cgtgatcatg cggcccggcg cgcaccggat
     1081 ccccggggtt cgcgactcca agacgctgtc ggcggcgcag cgcgaacgac tggccccgat
     1141 catccgccgt cgggcggtcg cgatcgggct cggcgccgcg tcggtgcgcg agatcgacgc
     1201 gatcaacatc tatcacgcca cccacctggc gatgcgccgc gcggtcggcc ggctcggcgg
     1261 gcacgaccac gtgctggtcg acggcaaccg gatcgccggg ttcgaggctg ccgtcgggcc
     1321 gtatacggcg atcgtcgatg gtgatgccaa gtgctacacg atcgcgtgcg cctcggtcgt
     1381 cgcgaaggtc gtgcgcgacc ggatgatgac cctgctctcc gcgcggtatc cgggctacgg
     1441 ctgggagcgc aaccagggct acgcgacgca ggagcatcgc gacgcgatcc gccgtctcgg
     1501 ccttacgccg ttccaccgac ggagcttcct cgccctgcag cgaaccctcg cgggcgacca
     1561 gatcagcctc gacctgttcg atgaggccgt tgcctcggtc gagcaggaag ccgacgtccg
     1621 ggacctggac cggctcgacg gctcaccggg ccttgccgag gcgcccgaca tcgccgtcgt
     1681 atcgggtttt ggcgacgtga cgggtttcgc cgatgttccg ggcttcggcg aagtgccggg
     1741 tcctggcgag gtgccgagcc tggacgagct ggccgagctg tccggcctgt ccgccgcaga
     1801 cctcgtcgtg acagcgcccg atccgagcgc tgccgcggcg atcgccgagg ccgaggcgct
     1861 cgtgcgcgag ctagccgatt cgccggcaaa ggccgggctc gactccggcg cctgatcctc
     1921 gccaacgtcc ggttgatctg ccggtaagcg gcaggtggtc ggcggttgat cccgaggtga
     1981 gcggcccgtg ccacgctgcc cgggatgcga accgctgccc agcagtcggg tgatgcggcg
     2041 gaggcgcggg tggcgggtcg tctcgagcag ctgggctggc agatcctcgc gacccacgtc
     2101 cgagttggac gggctgagct ggacctcgtc acgatcgatc ccgggccgcc cgcgacgctc
     2161 gtcatcgtag aagtccgctg gcgcggaagg cgcgacttcg gattgcccga ggagacggtc
     2221 gacggccgga agcgagctcg cctccatcga gccggtttcg cgctgcgtga acaggggcgc
     2281 ttgcccgacg gaagaccgct gccgccgcta ccgctccggt tcgacctcgt cgtcctcgag
     2341 ccgggcgacc gaatgcgcca ccaccggcac gcggcctgac gcccgaccgt gcatccaatg
     2401 cacgatcggg cgggcgaacc ggtcgaacga gccgcgaatc ccgccctcag ccgtcagatc
     2461 gaacatctgc tggacggaat gaccgaggag caagctcgaa gccacgatcg gagcgggttc
     2521 gaccgccgca tcgttcatct gatgaacgag cggagcgacg tcggcggcag ccgcgcctga
     2581 gggccgcccc gggatcccgt gctagactcc gccggccgcg ggaccgatcc cacggcaatc
     2641 aacgcccggc gctcgacacc aggtcgagaa tcggagctca cacgcggacc gtaccgatcg
     2701 ggacggtgcc ggacgagccc tgcggcgaac ccgcagcggc gaggacggtt cccgacgagg
     2761 gtccgcggcg gatacgaacc aggaggctac gcaaccgtgc cgaccatctc gatgcggcag
     2821 ctgctcgagg ccggagtcca cttcggccac cagacccgcc gctggaatcc caagatgaag
     2881 ccgttcatct ttgccgagcg caacgggatc cacatcatcg acctcgccca gaccgtccag
     2941 cggctggatg tcgccctcga ccatgtccgc gagaccgtcg cgcgtggcga ccaggtcctg
     3001 ttcgttggca ccaagaagca ggcccaggag ccggtcg
//
LOCUS       DATBSW010000139         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_610700, whole genome shotgun sequence.
ACCESSION   DATBSW010000139 DATBSW010000000
VERSION     DATBSW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610700"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VIF63_03875"
     CDS             <1..435
                     /locus_tag="VIF63_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238552.1"
                     /translation="DLGRLIERMATGTADVVVGYRIQRADPLVRTVYARLYRLANRIY
                     FGLKVRDVDCACKLFRRAALEGVAVESGGAFFSAELLIKLKALGRRIDEVGVPHYPRT
                     AGSPTGAKPQVVFRAVRDFWALRLRLWVSPSKALTRGTPILG"
     gene            complement(677..1285)
                     /locus_tag="VIF63_03880"
     CDS             complement(677..1285)
                     /locus_tag="VIF63_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY8238553.1"
                     /translation="MTRLVEMVIESVRVHMLSNRHVVILKDHAGDRYLPIWIGAWEAS
                     AIAMRLQGLTAERPLTHDLFAAALDRLGVRIVRVVISELSDETYHARLHLERDGVEVE
                     VDSRPSDALALAVRAEVQIFADEEVLAQAALAADPDEVSPDDDADDEPVIEGAERPRR
                     RRPPLESVGDAPADPRLDMFRDFVNSLEHDQGAGPESGGRRS"
     gene            complement(1293..2018)
                     /locus_tag="VIF63_03885"
     CDS             complement(1293..2018)
                     /locus_tag="VIF63_03885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8238554.1"
                     /translation="MIPLRDRNPTRRTPFVTLALIGACFIVFAVELLVTANGGDAALE
                     RFFRQWGAVPADITAALRTGDLFGSAMLGAFTSMFLHGGWAHILGNMLFLWIFGNNVE
                     DRLGPIPFLVFYLVGGLTAAFAQVVIDPQSTVPLVGASGAIAAALGAYIVLYPGAKIV
                     SLLFLGFIVQVLEVPALIVLGYWFLIQLVSGFASFGAETAQGGVAFFAHIGGFVLGAL
                     VGLVLRLAGDRTGRQGPVPGPMG"
     gene            complement(2015..>3662)
                     /locus_tag="VIF63_03890"
     CDS             complement(2015..>3662)
                     /locus_tag="VIF63_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238555.1"
                     /translation="AMVQLHPQSAVEALTTRFRAGGGTGSDGALVIEAAEIAVTRGGV
                     VPVPVRPAEPLAGAPDRSPIPLADSVVGGIAAAQSGARRARVATGGVLYRLLGRVQDA
                     LPNRAAPNRRVTPMSARREMQRRAAMALLSLVVVVGGLGAAVFFLGGRQPTGPTIASL
                     NAAQGAVDLAQANLKRVIGPGVDLVVNDPERAEELLSAAYTALETAERGNVPLSTINP
                     IRSQVVAALDRLYGMIDVASSPVFTFPDKPAVDLRTMVQGPDGAPFVLDAATQSVYRI
                     DIPGKKATVIFREGNKAAGSTQWAPKLLAVGGRDLLMLDTRNVMWRWQPANATGKGTI
                     TRVRVQGASEWGDDILGIGTFLRNRDDNLYNLYVVDPSAQQILRYSPAADGGGFPNVP
                     NLYLNAARDVGGISSVYIDGDVWLADGGEILRLVSGNSAGWSASLPGDAVLRDDPVYR
                     LISSGAERRTGTMYGYDPTSDRVVALSKVNGTFLAQYRLYGGATGWSDIRGWYVEPGL
                     EQEPDTLVWMGPKGIFRSVLQPTSGVPNASEGPPSPDASK"
BASE COUNT          548 a         1290 c         1309 g          515 t
ORIGIN      
        1 gacctcggcc ggctgatcga gcgcatggcg accggcacgg ccgatgtcgt cgtcggctac
       61 cggatccagc gcgccgaccc cctcgtccga acggtctacg cgcggctgta ccgcctggcg
      121 aaccggatct acttcgggct caaggtccgc gatgtcgatt gcgcctgcaa gctgttccgg
      181 cgcgccgccc tcgagggcgt ggcggtcgaa tcgggcgggg cgttcttctc ggccgagctg
      241 ctgatcaagc tcaaagccct cggccgtcgc atcgacgagg tcggcgtgcc ccactacccg
      301 cgcacggccg gcagcccgac cggcgcgaag ccgcaggtgg tcttccgcgc ggttcgcgac
      361 ttctgggcgc tgcgcctccg cctgtgggtc tcgccgagca aggcgctgac gcgaggcacc
      421 ccgatcctgg gctaacccct cgcgcgcgac cgaatgtccg cgtggaggac gagccggaca
      481 cgatctaccc gcgtagggtc gtccaggagc tcgaagcctg gccatttgtc cgcgtcgagg
      541 acaatcggag ccatcgcggg cctgaatcga ccgtcgcaat gcgtcccacc ggctgaatgc
      601 gtccgggttg tcctctgcgg tgacatcggt tcgcgccaag gcgcggcggc ggcccgctct
      661 cggggcccgc gcctgatcag gagcggcggc cgccgctctc ggggccggcg ccctgatcgt
      721 gctccagcga gttcacgaag tcgcggaaca tgtcgagtcg cgggtcggcc ggggcgtcgc
      781 ccacggattc gaggggcggt cgccggcgcc gcggtcgctc ggcgccctcg atgaccggct
      841 cgtcgtctgc gtcgtcgtcc ggggagacct cgtcgggatc ggcggcgagc gcggcctggg
      901 cgaggacctc ctcgtcggcg aagatctgga cctcggcgcg caccgccagc gcgagcgcgt
      961 cggacggacg cgagtccacc tcgacctcga cgccgtcgcg ctccaggtgg aggcgcgcgt
     1021 ggtacgtctc gtcggagagc tcggagatga cgacgcggac gatcctgacg ccgaggcggt
     1081 cgagcgccgc cgcgaacagg tcgtgggtga gcggccgctc ggcggtcagg ccctggaggc
     1141 gcatcgcgat cgcgctcgct tcccacgcgc cgatccagat gggcaggtag cgatcgccgg
     1201 cgtggtcctt gaggatcacc acgtgccggt tcgacagcat gtgcacgcgc acgctctcga
     1261 tgaccatctc gacgaggcgg gtcatggcgg gattatccca tcgggccggg cacggggccc
     1321 tgccgtcccg ttcggtcccc ggcgagccgc aggacgagcc cgaccagcgc gcccaggacg
     1381 aacccgccga tgtgcgcgaa gaacgccacg ccgccctgcg ccgtctccgc gccgaacgac
     1441 gcgaagccgc tgaccagctg gatcaggaac cagtagccga ggacgatcag cgccggcacc
     1501 tcgagcacct ggacgatgaa cccgaggaac agcagcgata cgatcttcgc gcccgggtac
     1561 agcacgatgt acgcgccgag cgcggccgcg atcgcgcccg aggcgccgac caggggcacc
     1621 gtcgactgcg ggtcgatcac gacctgcgcg aacgccgcgg tcaggccgcc gacgaggtag
     1681 aagaccagga aggggatcgg cccgagccgg tcctcgacgt tgttcccgaa gatccacagg
     1741 aacagcatgt tgccgaggat gtgggcccag ccgccgtgca ggaacatgct cgtgaacgcc
     1801 ccgagcatcg cggacccgaa caggtcgccc gtccgcagcg ccgccgtgat gtcggccggg
     1861 accgcacccc actgccggaa gaaccgctcg agggccgcgt cgccgccgtt cgccgtgaca
     1921 agcagctcga ccgcgaagac gatgaagcac gcaccgatga gcgcgagggt cacgaacggt
     1981 gtccgacgcg tcgggttccg atcgcgcagc gggatcactt gctggcgtcg ggtgacggag
     2041 gtccttccga ggcattcggg acgccgctgg tgggctggag gaccgagcgg aagatcccct
     2101 tcgggcccat ccacacgagc gtgtcgggct cctgctcgag ccccggctcg acgtaccagc
     2161 cccggatgtc gctccagccc gtcgcgccgc catagaggcg gtactgggcg aggaacgtgc
     2221 cgttgacctt cgacagcgcg acgacgcggt cgctcgtggg gtcgtacccg tacatcgttc
     2281 cggtgcggcg ctcggcaccc gaactgatca ggcggtagac gggatcgtcg cgcaggacgg
     2341 cgtcgccggg tagcgatgct gaccagccgg cgctgttccc gctcacgagc cggaggatct
     2401 cgccgccgtc ggcgagccag acgtcgccat cgatgtacac ggaactgatc ccgccgacgt
     2461 ctcgcgcggc attcaggtac aggttcggca cgttcggaaa gccgccgccg tcggctgccg
     2521 gcgagtaacg caggatctgt tgcgccgacg gatcgacgac atagaggttg tagaggttgt
     2581 cgtcgcggtt gcggaggaac gtgccgatgc ccaggatgtc gtcgccccac tcggaggcgc
     2641 cctgcacccg gacgcgcgtg atcgtgccct tgcccgtggc gttggccggt tgccatctcc
     2701 acatgacgtt cctcgtgtcg agcatcagca ggtcgcgccc gccgaccgcg agcagcttcg
     2761 gcgcccattg cgtggaaccg gcggccttgt tgccctcccg gaagatgacg gtcgccttct
     2821 ttcccgggat atcgatccga tacacgctct gcgtcgccgc gtcgaggacg aatggcgcac
     2881 cgtcgggccc ctggaccatc gtgcgcaggt cgaccgcggg cttgtccggg aaggtgaaca
     2941 ccgggctcga ggcgacatcg atcatgccgt acaaccggtc cagcgccgcg acgacctggc
     3001 tgcggatcgg gttgatcgtc gacaacggga cgttgcctcg ctcggccgtc tcgagcgccg
     3061 tatacgccgc cgacagcagc tcttccgccc gctccggatc gttgacgacc agatcgacgc
     3121 cggggccgat cactcgcttg agattcgcct gcgcgaggtc gactgcgccc tgggccgcgt
     3181 tcagggacgc gatcgtgggc ccggtgggct gacggccgcc gaggaagaac acggccgcgc
     3241 cgaggccgcc gaccacgacg acgagcgaca gcagggccat cgccgcgcgg cgctgcatct
     3301 cgcggcgagc gctcatcggc gtgacccggc gattcggcgc cgcccggttg ggcagggcgt
     3361 cctggacgcg accgagcagc cggtacagca cgccgccggt tgcgacgcga gcgcggcgcg
     3421 cccccgactg ggcggcagcg atgccgccga cgacggaatc cgcgagcggg atcggcgagc
     3481 ggtccggcgc cccggccagc ggctcggcag gtcgcacggg gactggcacg acaccgccgc
     3541 gggtgaccgc gatctccgcg gcctcgatga cgagcgcgcc gtcgctgccc gtcccgccgc
     3601 cggcccggaa gcgggtcgtg agtgcctcga ccgccgactg gggatggagc tggaccatgg
     3661 cg
//
LOCUS       DATBSW010000140         3989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1104992, whole genome shotgun sequence.
ACCESSION   DATBSW010000140 DATBSW010000000
VERSION     DATBSW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104992"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1158)
                     /locus_tag="VIF63_03895"
     CDS             complement(52..1158)
                     /locus_tag="VIF63_03895"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8238556.1"
                     /translation="MLTIETIRSAPKALLHDHLDGGLRPATIVDLAAEFGYKGLPTTD
                     VDDLGVWFRRGADRKSLELYLETFEHTIGVLQDRDAIIRVAAECAEDLAADGVVYAEV
                     RYAPELSTQKGLTLDEVVDANLEGFLLGSKRAAEAGRPVVMKALVTAMRQAARSVEVA
                     ECAVRWRDAGVVGFDIAGPEAGYRPTRHIEAFDKARHENFHITIHAGEGFGLPSIWEA
                     LQFCGAERLGHGVRIVDDIKVLADGSVELGRLAAFVRDRRVPLEMCPTSNVHSGAAES
                     VETHPIDLLRRLRFRVTVNTDNRLMSNVSMSSEMQALEQAFGIGLGEMEWLTINAMKS
                     AFAPFDERLRLINEVVKPGYAHLRAMEARVAIPG"
     gene            complement(1257..3251)
                     /locus_tag="VIF63_03900"
     CDS             complement(1257..3251)
                     /locus_tag="VIF63_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY8238557.1"
                     /translation="MLRTLRTIVAGSAIFLSLAGPVAAGTAPVGSLPRPSDASRFGWS
                     TAVDATGDLVVVGAPGIGNGQAPGRAYVYERAGGVFTRVATLRTTDDAPRNGFGSDIA
                     IDGEVIVVGSSNAFGVAAKAYVFVRPAAGWSGTLSETATLAPSASTVPPGFGMAVDMD
                     GQVIVVGDGNDVFVYTEPAGGWAGALTESAKLGRVSTVEIGRTVAIEGTVVITGDPVD
                     CTLCAVQVYERPSTGWTGTVSPVAKLLAPAGAFGFGTVAISDATVVVGASVEWVDGTE
                     RYAGGAYVFEKPAGGWTGSMAAAAALHASDASWYDGFGSAVTIDGDVIVVGNDSDATD
                     GTPNDGAYLFEKPAGGWTGELIQEQVIHRGSAVDVFGSSVSLGGGTLIVGSQLEGAPS
                     NFSGAAYAYLSDADRDFVADVIDNCPDDANRDQSDVDHDRAGDACDDDDDSDGVADAA
                     DNCALVANPDQIDLDGDAVGDACDFDDDNDTIGDPFDNCPRVANPDQANLDADADGDA
                     CDEDDDGDTVADASDNCLRLANPNQYDLDGDRTGDRCDVANTAGIDILPGQSPNVVKG
                     GAKLVAVSILSGPTFDAVARSDRATLRFGKTGAEAPIVGTCRVLDVNADGMADLTCDF
                     ELRKAAFAKKDTVGVLTGRTLGSEATSFSGTGSIFLVKPR"
     gene            complement(3281..3529)
                     /locus_tag="VIF63_03905"
     CDS             complement(3281..3529)
                     /locus_tag="VIF63_03905"
                     /inference="COORDINATES: protein motif:HMM:NF033706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO4226 family nickel-binding protein"
                     /protein_id="HEY8238558.1"
                     /translation="MIRFMDVHSGFFGVTAEQLEAAHQRDLDIEGDEGVHFERAWLDP
                     EAGKVFCLATGPTKEAVMRIHERAGHPTTEVYELPIEV"
     gene            3706..>3989
                     /locus_tag="VIF63_03910"
     CDS             3706..>3989
                     /locus_tag="VIF63_03910"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEY8238559.1"
                     /translation="MSEVGPHISPLLVGRDDLLALADRRIAEVAEGRGHVLLLSGEAG
                     IGKSRLLRAMIQKASMARFRYSKSDLAPQDRLVVLASLQDLARAMDVRDWG"
BASE COUNT          613 a         1374 c         1365 g          637 t
ORIGIN      
        1 cctcagcagg gcggggcggg gcgacgaccg ccgctcgccg ctaggaaacg ctcagccggg
       61 gatcgcgacc cgcgcttcca tcgcccgcag gtgggcgtac ccgggcttga cgacctcgtt
      121 gatcagccgg aggcgctcgt cgaacggggc gaaggccgac ttcatcgcgt tgatcgtcag
      181 ccattccatc tcgccgaggc cgatgccgaa ggcctgctcg agcgcctgca tctcgctcga
      241 catcgacacg ttcgacatga gccggttgtc cgtgttcacg gtgacccgga agcgcagtcg
      301 acggagcagg tcgatcgggt gggtctcgac cgactcggcc gcgccggagt ggacgttcga
      361 ggtcgggcac atctcgagcg gcacgcgccg gtcgcgcacg aacgcggcga ggcggcccag
      421 ctcgaccgag ccgtcggcga ggaccttgat gtcgtcgacg atgcgcacgc catggccgag
      481 ccgctcggcg ccgcagaact ggagcgcctc ccagatcgag ggcaggccga agccctcacc
      541 ggcgtggatc gtgatgtgga agttctcgtg gcgggccttg tcgaacgcct cgatgtggcg
      601 cgtggggcgg tagccggcct cgggcccggc gatgtcgaag ccgacgacgc cggcgtcgcg
      661 ccatcgaacg gcacactcgg cgacctcgac cgatcgcgcc gcctggcgca tcgcggtcac
      721 gagcgccttc atcacgaccg gacggcctgc ctccgctgcc cgcttcgagc ccagcaggaa
      781 accctcgagg ttggcgtcga ccacctcgtc gagggtcagc cccttctggg tcgagagctc
      841 gggcgcgtag cgcacctccg cgtagacgac gccgtccgcg gcgaggtcct cggcgcactc
      901 cgcggcgacg cggatgatcg cgtcgcggtc ctggaggacg ccgatcgtgt gctcgaacgt
      961 ctcgagatac agctcgagcg acttccggtc cgcgccgcgc cggaaccaca cgccgagatc
     1021 gtcgacatcg gtcgtcggca ggcccttgta gccgaactcg gcggcgagat ccacaatcgt
     1081 cgcgggccgc aggccgccgt cgagatggtc gtgaaggagc gccttcggcg cgctcctgat
     1141 ggtctcgatc gtcagcatcg ttcgcgggat ggtacgcgca gcgccggtcg cgaagaagcc
     1201 cgccggggcg gtgccgccga ggggccttcg tgcgtggctg ggaacagacg acgccgctag
     1261 cggggcttca ccaggaagat ggagcccgtg ccggagaacg acgtggcctc gctgccgagc
     1321 gtccgaccgg tcagcacgcc gaccgtgtcc ttcttcgcga acgccgcctt gcggagctcg
     1381 aagtcgcacg tgaggtcggc catcccgtcg gcgttcacgt cgaggacacg gcacgtcccg
     1441 acgatcggcg cttccgcgcc ggtcttcccg aaccgcagcg tcgctcgatc ggacctggcg
     1501 acggcgtcga acgtggggcc gctcaggatc gagacggcaa cgagcttggc gccgcccttg
     1561 accacgttcg gagactggcc gggcaggata tcgatgcctg ccgtgttcgc gacgtcgcat
     1621 cgatcgccgg tcctgtcgcc atccaggtcg tactggttcg gattcgccag ccgcaggcag
     1681 ttgtccgacg cgtcggcgac cgtgtcgcca tcgtcgtctt cgtcacaggc atccccatcc
     1741 gcgtcagcgt cgaggttggc ctggtcgggg ttcgcgacgc gggggcagtt gtcgaacggg
     1801 tcgccgatgg tgtcgttgtc gtcgtcgaag tcgcaggcgt cgccaaccgc gtcgccgtcg
     1861 aggtcgatct ggtcggggtt cgcgacaagg gcgcagttgt ccgccgcgtc agcgacccca
     1921 tccgagtcgt cgtcgtcatc gcacgcatcc cctgctcgat cgtgatcgac gtccgactgg
     1981 tcgcggttcg cgtcgtctgg acaattgtcg atgacgtccg cgacgaagtc gcgatcggcg
     2041 tccgacaggt aggcatatgc cgcaccagag aagttgctgg gcgcgccctc gagctggctc
     2101 ccgacgatca gcgtcccgcc gccgagcgac acggaggagc caaagacgtc gaccgcggat
     2161 ccacggtgga tgacctgttc ctggatcaac tcgccggtcc agccgccggc gggtttttcg
     2221 aacaggtagg cgccgtcgtt cggggttccg tccgttgcgt cggagtcgtt gccaaccacg
     2281 atcacgtcgc catcgatcgt cacggccgag ccgaatccgt cataccagct ggcatcgctc
     2341 gcatgcaggg ccgctgctgc tgccatggag ccggtccacc cgcccgcggg cttctcgaag
     2401 acatacgcac caccggcgta ccgttcggtt ccgtcgaccc actcgaccga ggcgccgaca
     2461 acgacggtgg catcgctgat cgcgaccgtg ccaaagccga aggcgccagc cggtgcaagc
     2521 aactttgcga ccgggctgac ggtgccggtc catcccgtcg acggtcgctc gtagacttgg
     2581 acggcgcaga gcgtgcagtc gaccgggtcg ccggtgatga cgaccgtacc ctcgatcgcc
     2641 accgtgcggc cgatctcgac cgtggagaca cgcccgagct tcgccgattc ggtgagcgcc
     2701 cccgcccagc cgcccgctgg ctctgtgtag acgaacacgt cgttcccatc gcccacgacg
     2761 atcacctggc catccatgtc cacggccatg ccaaagcccg gcggaacggt gcttgcggac
     2821 ggggccaggg tggcggtctc cgagagggtg cccgaccagc ctgccgccgg gcgcacgaag
     2881 acatacgcct tcgctgccac accaaaggcg tttgaagagc cgacgacgat gacctcgcca
     2941 tcgatggcga tatcgctgcc gaatccattc cttggcgcgt cgtcggtcgt gcgcagggtt
     3001 gcaacgcgcg tgaacacgcc gccggctcgc tcgtagacgt aggcgcggcc gggcgcctgc
     3061 ccgttgccga tccccggcgc tccgacgacg acgagatcgc cggttgcatc gaccgccgtg
     3121 ctccagccga agcggctggc gtcggacggg cgcggcagcg acccaaccgg cgccgtaccg
     3181 gccgcgaccg ggccggcgag ggacaggaag atcgccgatc cggcgacgat ggttcgaagc
     3241 gttcggagca tgatcacggc ctccaatcgg cgtgttgcgg tcagacctcg atggggagtt
     3301 cgtagacctc ggtcgtggga tggccggcgc gctcgtggat gcgcatcacg gcctccttcg
     3361 tcggcccggt cgcgaggcag aagaccttgc cggcctccgg atccagccag gctcgctcga
     3421 aatgcacgcc ctcatcaccc tcgatgtcga ggtcgcgctg gtgtgccgct tcgagctgct
     3481 cggcggtgac gccgaagaac ccggaatgga cgtccatgaa cctgatcacg aaaggcctcc
     3541 tcagagccgt gtcttgttgg cagctcatag gctctccagg aaggggtctg ccgccatcgg
     3601 tcatccgacc gatccaccgt ccggggcccg accgatttcc ggggcgggcg gggtatcggt
     3661 cattcgaccg atgcatctct ggaagccact cgtacactcg tgtgcatgtc cgaggtcggg
     3721 ccgcacatct cgccactcct cgtcggccgt gacgacctgc tcgccctggc cgaccggcgg
     3781 atcgcagagg tcgccgaagg ccgcggccac gtcctgctcc tgtcgggaga ggcgggcatc
     3841 ggcaagagcc ggctcctgcg agcgatgatc cagaaggcca gcatggcgag attccggtat
     3901 tcgaagagcg acctcgcgcc gcaggaccgg ctggtcgtgc ttgcgtccct ccaggacctg
     3961 gcgcgcgcga tggacgtccg ggactgggg
//
LOCUS       DATBSW010000141         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_223042, whole genome shotgun sequence.
ACCESSION   DATBSW010000141 DATBSW010000000
VERSION     DATBSW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223042"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VIF63_03915"
     CDS             complement(<1..260)
                     /locus_tag="VIF63_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY8238560.1"
                     /translation="MFERTALADGPRVITAPLPAARSVSIAAYVRAGSRLEAPEQAGM
                     AHFLEHLTFKGTAAYPSTRALSEAIEGVGGSFNAATDREATV"
     gene            361..1497
                     /locus_tag="VIF63_03920"
     CDS             361..1497
                     /locus_tag="VIF63_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238561.1"
                     /translation="MTGLEVAGLNRRTVALLAAAAPLGMGAILVLVGSWIDDRDNRLL
                     VAPTDAPSAASPGPSTAVTPAPSVTIAAGDIHLVPETTALFAAPLPGNWRPLDVTEIN
                     EGGYRIVTDSELGPSWDPVSVAWITISASSETMESAQAEMQAAGWTAPPQVVDVPGLE
                     GIVLSADPIGGVAQGRDPTALIVWRGATYRISAHNKKSPGLRQVELIRFLRFFRPLGF
                     AADAEQYRDEVWRFTFVNIASGGRFVPGLELGNGVTRFGDVTCGSTDASCKTFVDVVR
                     VPSGSLLRLTLPDERDVALAIDPSSDDYVTLAADWETVVGKSRGSSWIFWQGTPIYDV
                     DNLTLGARFIHRGDQWLVILSSEAEPGGQTSRLDAFLQGLTLLP"
     gene            1580..2134
                     /locus_tag="VIF63_03925"
     CDS             1580..2134
                     /locus_tag="VIF63_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8238562.1"
                     /translation="MSEELVVRARGGDHAAFEQLAAASIDRLYAIARRVLRDQTSAED
                     AVQEALVHAWRDLRALRDPGRWEAWLYRLLLHACRDEQRRSGRRPQTVEIGLLGGGAT
                     PDASDAVGRRDQLERGFSRLSVDHRMVLSLHFFGGLRPAEIAELLGIPTGTATSRIHY
                     GSRALRAAIEADLVPSTAPTGAVK"
     gene            2131..>3230
                     /locus_tag="VIF63_03930"
     CDS             2131..>3230
                     /locus_tag="VIF63_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238563.1"
                     /translation="MTNEPNPPIERQMAALLEDGPTTAPSGLLASTLRQTGALRQRPG
                     LAARALGAPGAGTGANPVASVRLIWLIAALALLALLAVAVVAGQALWRENPLVVVMTD
                     SPLATPRPSIPAATRGPIVAPTPAVTPDPMEGMAELGFTVAPLGAFERVTWSGPGAFG
                     DSCAEGTDFCAAMVRVHVGDVATGVNLGGSEDPNILHGRTAAELATAWVDQIGRPSTV
                     ESLVVDGIPGRLVETPGSDDETVPPEARAFVVVSGRVFAWEAYDLFGKAGTVLRQFLP
                     SVHFLPAGCWLTPCEADPGGWLEPHPLVAFDPAAATGDWTANHGWASGPAPDGSVRGF
                     WLGSCADNLCKGYVSVSVGTTATGAFVARTSF"
BASE COUNT          455 a         1131 c         1120 g          524 t
ORIGIN      
        1 tacaccgtcg cctcgcggtc cgtggccgcg ttgaacgagc cgccgacacc ctcgatcgcc
       61 tcgctcagcg cccttgtcga tggatacgcc gcggtgccct tgaaggtgag gtgttcgagg
      121 aagtgggcca tgcccgcctg ctcgggcgcc tcgaggcgcg atccggcccg gacgtacgca
      181 gcgatcgaca ccgatcgcgc ggcgggcagg ggcgcggtga tcacgcgcgg cccgtcggcc
      241 agcgccgtcc gttcgaacat cgccgggatg gtacccgtct gccttcgatt gatggaatcg
      301 cggcggcccg acgccatact ccgttagcgg cccgcagggt cgccttccca gggaggggag
      361 atgacggggc tcgaggtagc ggggctgaac cgccggaccg tggcgctcct cgcagccgcc
      421 gcgcccctcg gcatgggggc catcctggtc ctcgttggca gctggatcga cgaccgggac
      481 aatcggctgc ttgtcgctcc gacggacgca ccgtccgcgg ccagcccggg tccatcgacc
      541 gctgtcacgc cggcgccctc cgtcacgatc gctgcgggag acattcatct cgtccctgag
      601 acgacggccc tcttcgcggc cccactcccc ggcaactggc gtccgctcga cgtgaccgag
      661 atcaacgaag ggggctaccg catcgtgacg gattcggagc tcgggccgtc ctgggatccc
      721 gtaagcgtgg cctggatcac gatcagcgcg agcagcgaga cgatggagtc ggcgcaggca
      781 gagatgcaag cggccggctg gacagcaccg ccccaggtcg tcgacgtgcc tggcctggag
      841 gggatcgtcc tctccgctga cccaatcggt ggcgttgctc agggccgcga ccccacggcc
      901 ctgatcgtgt ggcgcggcgc gacgtatcgg atctcggccc acaacaagaa gagtcccggc
      961 cttcgacagg tcgagctcat tcggttcctc cgattctttc ggcccctcgg attcgcggct
     1021 gacgccgagc aataccggga cgaggtctgg cggttcacat ttgtgaacat cgcctcggga
     1081 ggccgcttcg tgcccggact cgaactcggg aatggggtga cccggttcgg agatgtcacg
     1141 tgcggctcga ccgacgcgag ctgcaagacc ttcgtcgacg tcgtccgcgt tccgagtggc
     1201 tcgctccttc gtctcacctt gccggatgag cgcgacgtcg cgctcgccat cgatccctcc
     1261 tccgacgact acgtcacgtt ggccgccgac tgggagaccg tggtcggcaa atctcgcggc
     1321 tcgtcatgga tcttctggca gggaacgccg atctatgacg tcgacaacct cacgctcggc
     1381 gcccggttca tccaccgagg ggatcagtgg ctcgtgatcc tctcgtccga ggccgagccg
     1441 ggtggacaga cgtcccgcct cgacgcgttc ctccagggtc tgaccctgct cccgtgagag
     1501 aagtgcgatt gatggaatcg aggccgctcg acgccatact ccgcgaacgg tcgatgccga
     1561 ccgtcccctg gaggggtggg tgagcgagga gctggtggta cgggcccggg gcggcgatca
     1621 tgccgccttc gagcagctcg ccgcggcctc gatcgaccgc ctctatgcga tcgcccggcg
     1681 ggtcctgcgt gaccagacgt cggccgagga tgccgtccag gaggccctcg tacatgcatg
     1741 gcgcgacctg cgtgcgctgc gcgacccggg ccgatgggag gcatggctct atcgcctgct
     1801 tctgcatgcc tgccgcgacg agcagcgccg gtccggtcgc cgtccgcaga cggttgagat
     1861 cgggctgctc gggggaggcg cgacacccga tgccagcgac gcagtggggc gccgtgacca
     1921 gctcgagcgc ggcttcagcc ggctgagcgt cgatcatcgg atggttctct ccctgcattt
     1981 cttcggcggg ctgcgacccg ccgagatcgc cgagctcctc ggcatcccca ccggcacggc
     2041 gacgtcccgt atccactacg gcagccgcgc cctccgggct gcgatcgagg cagacctcgt
     2101 tcccagcacc gcaccgacgg gggcagtgaa gtgaccaacg agccgaaccc gccgatcgag
     2161 cgtcagatgg ccgcactgct cgaggacggg ccgaccacgg cgccgagcgg cctgctcgcc
     2221 tcgacgctcc ggcagacggg cgcgcttcgt cagcgtccgg ggttggctgc gcgagcgctc
     2281 ggcgcgccgg gagccggcac cggcgccaat cccgtcgcat ccgttcggct gatctggttg
     2341 atcgcggcgc tcgccctcct ggcgctgctg gccgtcgcgg tcgtggcggg gcaggcgctc
     2401 tggcgggaga atccactggt cgtcgtcatg acggacagcc cgctcgcaac cccgcgcccg
     2461 agcatcccag cggcgactcg cggaccgatc gtggccccca cccctgctgt cacgcccgat
     2521 ccaatggagg ggatggccga gctcgggttc acggtcgcgc cgctcggggc cttcgagcgg
     2581 gtcacctgga gcggtccggg cgcgttcggc gacagctgcg ccgagggaac cgacttctgt
     2641 gccgcgatgg tccgcgtcca cgtcggcgac gtcgcaaccg gtgtcaacct gggcggctcc
     2701 gaggacccga acatcctgca cggccggaca gcggccgagc tcgcaacggc ctgggtggac
     2761 cagatcggcc gcccgtcaac tgtcgagtca ctcgtcgtcg atggcatccc tggccgactc
     2821 gtggagacac cggggtcgga tgatgagacg gttccgcccg aggcccgagc gttcgtggtc
     2881 gtctccgggc gtgtcttcgc ctgggaggcg tacgacctgt tcggcaaggc aggcacggtg
     2941 ctccgccagt tcctgccgtc cgtccacttc ctgcccgcag gctgctggct gacgccgtgc
     3001 gaggccgatc ccggaggctg gctcgagccg catccactcg tcgcgttcga tccggccgcg
     3061 gcgacaggcg attggaccgc gaaccacggc tgggcttcag ggccggcgcc tgacggctcg
     3121 gtgcgcgggt tctggctcgg gagctgcgcg gacaacctgt gcaagggtta cgtgtcggtc
     3181 tcggtcggga cgacagcaac gggcgccttc gtggctcgaa ccagctttga
//
LOCUS       DATBSW010000142         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_716603, whole genome shotgun sequence.
ACCESSION   DATBSW010000142 DATBSW010000000
VERSION     DATBSW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716603"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VIF63_03935"
     CDS             complement(<1..1338)
                     /locus_tag="VIF63_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8238564.1"
                     /translation="MSAQSALFKGLLTIVQVALIGTAVLVTPGLALASGQGPSGASSD
                     AQAAARCGFVPLDVEFIIDTSGSMGSNSSGTPPQTRMYWAKAAATQLVNALDAPGNGG
                     VGGATGIHHVGVTRFSGTTASVVGALGTATATTVNGWISGLSATGNTPLQTGMATGAG
                     DMNANARNAVGGAVQRIFILLTDGRPNPDLGPNGTWATTGVNNQRPTDPEGTAYLGSA
                     DIAYSIAIGAGGSGSSAVDLGLMQLLDKPNGRYHNVVDADDLPALFDDIFTEISCPPH
                     LVVEKTVNKAAADPGDTLNYTISLTNDGGSNATGVAVHDDISALLAHGTFGTCDNGCT
                     NDADSVDWSGLSVAVGATLNLHFSINLSGSFPAGTTDLPNTVLVASTNCSADTQDPDC
                     STNTTVTVAKNPHLSIDKVDNTGSFNKVDDVISYTITATNDGNVTLHNVGVTDD"
     gene            1952..2452
                     /locus_tag="VIF63_03940"
     CDS             1952..2452
                     /locus_tag="VIF63_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8238565.1"
                     /translation="MRHAAQSSGILLFRRTATGIEVLLAHPGGPYWKSQDDGSWSVPK
                     GVAEDGEALEAVAVREFGEETGFEVAAVARDAATPHLDLGEVTLKSGKVIRAWAVEGD
                     LDPALCHSNEIEIEWPPRTGRRLTIPEVDRLAWFGLDEARRRAHPVQAEFIDRLEGLL
                     GETPAT"
     gene            2449..3780
                     /locus_tag="VIF63_03945"
     CDS             2449..3780
                     /locus_tag="VIF63_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8238566.1"
                     /translation="MSEVREVSAAVARRFLVLRHLLAPARSLPAEPESVLRVIDRLGS
                     LQFDPLEVAGRNHDITLLARVAGYRREWTDHWLYEDRRLYETYNKSLQIVPVAELPLY
                     RWTWDRMHARHEAGAFDEHAPLVEELLGRIRDLGTLLSRDVKARESIDWYWRPTNQVR
                     AILEALAESGRLGIARREGNIRVYDLAERLFPPEILAARRPEPNQQEHRLLSRYRANG
                     LLGASGNQELWVGGTGYAADRKAVRERLMADGRLTRVQVEGLRGERFIVSEDVDFMND
                     AEREIREGAPPGGAEPTAAFIAPLDPFCWDRDLLRRLLDFDYVWEVYVPQPKRRWGYY
                     VLPILYGDRFVGRIEPRIDRKEGALRILGLWWQPDFDPMAEDGFAPAFARALEAHRDF
                     AGLQGVAMASAAGHRPFAKVVATHLGASKAMASSDRSGASRAPARRGGRSG"
BASE COUNT          596 a         1254 c         1342 g          663 t
ORIGIN      
        1 gtcgtcggtc acgcccacgt tgtggagcgt gacgttgccg tcgttggtcg cggtgatggt
       61 gtagctgatg acgtcatcga ccttgttgaa cgagcccgtg ttgtcgacct tgtcgatcga
      121 cagatgcggg ttcttcgcca cggtgacggt cgtgttggtc gagcaatcgg gatcctgcgt
      181 gtccgccgag cagttggtgc tcgcgacgag gaccgtgttc ggcaggtcgg tcgttccagc
      241 ggggaagctc ccgctcaggt tgatcgagaa gtggagattc agggttgcgc ccaccgcgac
      301 actgagaccg ctccaatcga cggaatcggc gtcgttggtg cagccgttgt cgcaggtgcc
      361 gaaggtgccg tgtgccagga gcgcgctgat gtcgtcgtgc actgcgacgc cggtggcgtt
      421 ggacccgccg tcgttcgtca ggctgatggt gtagttcagg gtgtcgccgg gatcggccgc
      481 cgccttgttc acggtctttt ccacgacgag gtgcggtggg caggagatct cggtgaagat
      541 gtcgtcgaac agggccggca ggtcgtcggc gtcgacgacg ttgtggtacc ggccgttggg
      601 cttatcgaga agctgcatca ggccgaggtc gacggcgctc gagcccgacc cacctgcgcc
      661 gatcgcgatg gagtaggcga tgtcggccga gccgaggtag gcagtacctt cgggatccgt
      721 aggacgctgg ttgttgacgc cggtcgttgc ccaggtgccg ttcgggccca ggtcggggtt
      781 cgggcgcccg tccgtgagga ggatgaagat ccgctggacg gccccgccga cggcgtttcg
      841 ggcgttcgcg ttcatgtcgc cggcgcccgt cgccatgcca gtctggagcg gcgtgttgcc
      901 ggtcgcgctg agaccgctga tccagccatt cacggtcgtc gccgtggcag tcccgagggc
      961 cccgaccact gaagcggtcg tccctgagaa gcgggtcacg ccgacgtgat ggataccggt
     1021 tgcaccgccg acgccaccat tgccgggtgc gtcgagtgcg ttcacgagct gggtcgcggc
     1081 ggccttggcc cagtacattc gggtctgtgg cggcgtgccg ctcgagttgc tgcccatgct
     1141 gccggaggtg tcgatgatga actcgacatc caggggcacg aagccgcaac gagccgcggc
     1201 ctgggcgtcg ctgctggcgc cgctggggcc ctgaccactg gccagcgcaa ggccaggggt
     1261 gacgagcacc gcggtgccga tcagggccac ctgaacgatc gtcaagaggc ccttgaagag
     1321 cgcggactgc gcgctcacgg gccgattcag cgacattatc gaacttcctc cctcaaaatg
     1381 acctgtcgga cgatgaggct tgccgaacgg gcaaagagcc cgtcgggacg ttcgcaccac
     1441 gggctcattt cacaccgggg agatggtttc gtcggacaga ccggcagcaa ctgcctgcgc
     1501 cgggcgcagc ccgacgcgca agaagcggca aacgcccagt ccgacatgcg atgtgtcccc
     1561 cgatcccctc ttgcgagggg cccccgtctt cgtgccttcc ccattccgac ccgatcgcag
     1621 aacgtctgac agatcggttc tcggtctctt gagtcttgga gtacgaaagt actggcgtca
     1681 aggtggttct ttcgtttggc gcgagaattg gttgcaacct cgcaccaccc aagggacgtt
     1741 caaggattac tggtggtgca cttcagggct aactggtgta gttcagggat aacttgtgca
     1801 cttcgcaatt gctgcgccag cgcatcaatg aaagaacgtt gcaccttctc gcgatcagcg
     1861 agccgagggc gcgtggccgg cgtcgctccg ttacgaactg gccgccgggg cgcgcaaccc
     1921 gcgtggcctc gcctgtgcga cgatccgccg catgcgacac gcggcccagt cgagcggcat
     1981 cctgttgttt cgacgaaccg cgacagggat cgaggtgctg ctcgcgcatc ccggcgggcc
     2041 gtactggaaa tcgcaggacg acggcagctg gagcgtgccg aagggcgtcg ccgaggatgg
     2101 cgaggcactg gaggcagtcg ccgtccggga gttcggcgag gagacgggct tcgaggtcgc
     2161 cgccgtcgcc cgcgacgcgg caacgccgca cctggacctc ggtgaggtga cgctcaagag
     2221 cggcaaggtc atccgcgcgt gggccgtcga gggcgacctt gacccggcgc tgtgtcactc
     2281 caacgagatc gagatcgagt ggccgccgcg gaccggtcga agactcacca tccccgaggt
     2341 cgaccggctg gcctggttcg gcctcgacga ggcgcgtcgg cgcgcccatc ccgtccaggc
     2401 agagttcatc gatcgccttg aaggcctgct cggcgagacg cccgccacgt gagcgaggta
     2461 cgggaagtct ccgcggcggt cgctcgccgc ttcctcgtcc tgcgtcacct gctggcgccg
     2521 gcccgctcgc tacccgccga gccggagagc gtgctgcgcg tcatcgaccg cctcggctcg
     2581 ctccagttcg acccgctcga ggtcgccggc cgcaatcacg acatcaccct gctcgcgcgc
     2641 gtcgccggct atcgccgcga gtggaccgac cactggctgt acgaggaccg gcgcctgtac
     2701 gagacgtaca acaagagcct ccagatcgtg cccgtcgcgg agctcccgct gtatcgctgg
     2761 acgtgggatc gcatgcatgc ccggcacgaa gccggcgcgt tcgacgagca cgcgccgctg
     2821 gtcgaggagc tcctcggccg gatccgcgat ctcggcacgc tgctatccag ggacgtcaag
     2881 gcccgcgaat cgatcgattg gtactggcgg ccgacgaacc aggtccgggc catcctcgag
     2941 gccctcgccg aatccggtcg cctgggcatc gcccggcgcg agggcaacat ccgcgtgtac
     3001 gaccttgccg agcgcctctt cccgcccgag atcctcgcgg cgcgccggcc cgagcccaac
     3061 cagcaggagc accggctgtt gtcccgctac agggccaacg gcctgctcgg cgcgtcaggc
     3121 aaccaggagc tgtgggtcgg tgggacgggc tacgccgcgg atcgcaaggc ggttcgggaa
     3181 cggctcatgg cagacggtcg gctcacccga gtgcaggtgg aaggtctgcg cggcgagcgc
     3241 ttcatcgtca gcgaggacgt cgacttcatg aacgacgccg agcgcgagat tcgcgagggc
     3301 gcgccacccg gtggcgcgga gccgaccgcg gccttcatcg cgccgctcga tccgttctgc
     3361 tgggatcggg acctgctgcg ccggctgctg gacttcgact acgtctggga ggtgtacgtc
     3421 ccgcagccga aacggcgctg gggctactac gtcctgccga tcctttacgg tgaccgcttc
     3481 gtggggcgta tcgagccgcg gatcgaccgc aaggaaggtg ctttgcggat cctgggattg
     3541 tggtggcagc ccgacttcga tccgatggcc gaggatggct tcgcgccggc attcgcgcga
     3601 gccctcgagg cccaccggga cttcgccgga cttcagggcg tcgccatggc gtcagccgcc
     3661 gggcatcgac cgttcgccaa agtcgtcgca acgcacctcg gcgcgtcgaa ggctatggcg
     3721 tcaagcgata gaagcggagc atctcgagcg ccggctcgtc gaggcggtcg atccggctga
     3781 atccggcctc cgcagccaag ccacgcatca tgtcctcgcg catcaccgtg ccgatcgccg
     3841 cgctcgggcg ctcgg
//
LOCUS       DATBSW010000143         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_152385, whole genome shotgun sequence.
ACCESSION   DATBSW010000143 DATBSW010000000
VERSION     DATBSW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152385"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VIF63_03950"
     CDS             <1..745
                     /locus_tag="VIF63_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238567.1"
                     /translation="RVEWAVYSLLSTAGPIAETAFFERVAALFSGHDLPDETLVRACL
                     ASYRSPASTPSRLVTSEDLRKRTDEHTELLAHLANGGHRLGMRVWIGRRDQARRIGDR
                     TLADFLDYREREPWFPTISLVPKEDGEAIDCVWYLRGGSAFLFEVEWTAMLGDVLLRR
                     HARIPPDPGIVRFLAIAPERRDLVRHKLESSPVLREAMEAANWHVILWPSLRAWLARE
                     TLSLADLEPYLGLDPVVERRAEQLDLFGS"
     gene            1062..>2532
                     /locus_tag="VIF63_03955"
     CDS             1062..>2532
                     /locus_tag="VIF63_03955"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8238568.1"
                     /translation="MPEPTAVDQLADSFWERFLELSPISATVNGDTRYDDRLPDPSPE
                     GRAKERKFAEDMRDAALAIPDEGLSVEDRITRDVLRVIGEIFVVQDDQRIDTLQVVDQ
                     MGGPQQLLPQVTQFQKADTPERLELFLARLHAYPQYMADNVALVGEGLASGLTAPRIV
                     AERTIAQLERMLATPIDQAVVPSLITVANDEDREAIRDVIRDEVQPAIAGFLEALKGD
                     YLAATRPDNGLWSAPNGDELYRTQILRWTTLDLDPQTVHQTGLDQLEGIESERREISR
                     KAGFGDDTVAYRASLAADEANQARSQAHLIARATDDIERAFELAPSMFRRHPKSQCRV
                     IPVEEYKERDAPFAYYYPPSADTTRPGTYYVNTFDLPSRTFSRLAATTYHEAIPGHHF
                     QISLESENTALNKFRRFAARYAGGAYVEGWGLYAERLADEMGLYEDDLSRMGMLTYQA
                     WRACRLVVDTGLHAKGWTRERAIEFMVANAPLSREEITNE"
BASE COUNT          416 a          946 c          813 g          357 t
ORIGIN      
        1 ccgcgtcgaa tgggctgtct acagcctcct gtcgaccgcc ggcccgatcg ccgagaccgc
       61 gttcttcgag cgcgtggcgg cgctcttctc cggccacgac ctgccggacg aaacgctcgt
      121 cagagcctgc ctcgccagct accgcagccc ggcgtcaacg ccgagccgcc tcgtgacctc
      181 cgaggatctc cgcaagcgaa ccgacgagca caccgagctc ctcgcgcatc tcgccaacgg
      241 cgggcaccgg ctcgggatgc gcgtctggat cgggcggcgc gaccaggcgc gccggatcgg
      301 tgatcggacc ctcgccgact tcctcgacta ccgcgagcgc gagccctggt tcccgacgat
      361 ctcgctcgtg ccgaaggagg acggcgaggc gatcgactgc gtctggtacc tgcgcggcgg
      421 ctccgcattc ctgttcgagg tcgagtggac agccatgctc ggcgacgttc tgctgcgccg
      481 ccacgcccgg atcccgccgg acccggggat cgtgcgattc ctggccatcg ccccggagcg
      541 gcgcgacctc gtccggcaca agctcgagtc gtcaccggtg ctgcgcgagg ccatggaagc
      601 ggcgaattgg cacgtgatcc tctggcccag cctccgtgcc tggctggcgc gcgaaaccct
      661 gagcctcgcc gacctcgagc catacctcgg gctcgacccc gtggtcgaac ggcgcgccga
      721 gcagctggat cttttcggct cgtagccccg tcgtgctggc gaatacctgg cgaagtacct
      781 gtcgaacgac ctgcagaagt gcctgacgaa tcacctggca gggataggat ttgcaccgag
      841 cccgtcggcg ctatccctct gaggcataga tcggcgcacg tgcggccgcc agcggccatt
      901 gccggcgcga aactcggtcg tccgtatacc tgccagggat cgcggcgacc ttacccctga
      961 aaaacctgtc cctctcggac acacggccgc cacggatgac cgaaccgcta ccctgatcta
     1021 cccggggccc gcgcgtcagc cccgaagctg gaggcaggac gttgcccgaa ccgaccgccg
     1081 tcgaccagct cgccgattcg ttctgggagc gattcctcga gctgagcccg atcagcgcga
     1141 ccgtcaacgg cgacacgcgc tacgacgaca ggctgcccga cccgagcccg gaaggccgcg
     1201 cgaaggaacg caagttcgcc gaggacatgc gcgacgccgc gctggcgatc ccggacgagg
     1261 gcctgtcggt cgaggaccgg atcacgcgcg atgtcctgcg cgtcatcggc gagatcttcg
     1321 tcgtccagga cgaccagcgg atcgacacgc tccaggtcgt cgaccagatg ggcgggccgc
     1381 agcagctgct gccgcaggtc acccagttcc agaaggccga cacgccggag cggctcgagc
     1441 tgttcctcgc ccgcctccat gcgtacccgc aatacatggc ggacaacgtc gcgctggtcg
     1501 gcgaaggcct cgcgagcggg ctgaccgcgc cgcgcatcgt cgccgagcgc acgatcgccc
     1561 agctcgagcg gatgctggcc acgccgatcg accaggcggt cgtgccctcg ctcatcaccg
     1621 tggcgaacga cgaggaccgg gaagcgatcc gggacgtgat ccgggacgag gtccagcccg
     1681 cgatcgccgg cttcctcgag gcgctgaagg gcgactacct cgccgcgacg cgccccgaca
     1741 acgggctctg gtccgccccg aacggcgacg agctgtaccg aacccagatc ctgcgctgga
     1801 ccacgctcga cctcgatccg cagaccgtcc accagaccgg cctcgaccag ctcgagggca
     1861 tcgagtcgga gcggcgcgag atctcgcgca aggccggctt cggcgacgac accgtggcct
     1921 accgcgcgtc gctcgccgcc gacgaggcga accaggcccg ttcccaggcc cacctcatcg
     1981 cccgcgcgac cgacgacatc gagcgcgcct tcgagctcgc gccgtcgatg ttccggcgcc
     2041 acccgaaatc gcaatgccgg gtcatcccgg tcgaggagta caaggagcgc gacgcgccgt
     2101 tcgcgtacta ctacccgccg tcggccgaca cgacccgccc cggcacctac tacgtgaaca
     2161 cgttcgacct gccgtcgcgg acgttctcgc gcctcgccgc gacgacctat cacgaggcga
     2221 tcccgggcca ccacttccag atctcgctcg agagcgagaa cacggctctg aacaagttcc
     2281 gtcgcttcgc cgcccgctat gccggcggtg cctacgtcga aggctggggc ctctacgccg
     2341 agcgcctcgc cgacgagatg gggctctacg aggacgatct ctcgcgcatg ggcatgctca
     2401 cgtaccaggc ctggcgcgcg tgccgtctcg tcgtcgacac cggcctgcac gcgaagggct
     2461 ggacccgcga gcgcgcgatc gagttcatgg tcgccaacgc cccgctctcg cgggaggaga
     2521 tcaccaacga gg
//
LOCUS       DATBSW010000144         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_541005, whole genome shotgun sequence.
ACCESSION   DATBSW010000144 DATBSW010000000
VERSION     DATBSW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541005"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VIF63_03960"
     CDS             <1..553
                     /locus_tag="VIF63_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEY8238569.1"
                     /translation="DRHAIRSRLRRLNDLGFAVDEIEIVPQGTGSEVNLRVTTSNRAF
                     HAHELERLTGLVALEGQAQLLLNDLREHTAWLRLRTGRTTDERVGARRWRRHVLDPAL
                     RLLRPAVGDRDLIQAYSDVLEHKWLLSERAGRDVGLKAAIEAYLAVGAPAPEVPTPDE
                     PGSKATLMDDSLEDDPLVDERGD"
     gene            complement(555..1076)
                     /locus_tag="VIF63_03965"
     CDS             complement(555..1076)
                     /locus_tag="VIF63_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8238570.1"
                     /translation="MDVWTAIRTKRMVRRFEDRPLDPAHLTRIVDAGRHAGSSKNQQA
                     WDFVVIEARETLVRLSTVGPYAGHVAGAAAAVALVTPDPTKPGASLSLTWDSGLAAEN
                     MLLAAWELGVGGCPATVYEPKLASEILGIPEGRYCGYILSFGYPAEPADLVRPPEAGG
                     RRPLDSVVHRERW"
     gene            complement(1076..2182)
                     /locus_tag="VIF63_03970"
     CDS             complement(1076..2182)
                     /locus_tag="VIF63_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8238571.1"
                     /translation="MLRRRLGLTAALTVGPIAAWRFAQAYRARAGYPHRHPPDHDPSF
                     LGLAFESLTVPSGDLDLPAWWIPARGGKPGPAVLLVHGWESARDRTLPNALILNAIGI
                     HVLTIDIRAHGENPPEELPLTAGEFGLDALAGVRALLARKDVTAVGVFGHSMGGIGAL
                     LAAAAEPRVAAVVAASTPADPYRLTRQTFRLAKLPIPGPFAWPLAWLTTHVFLEPRGH
                     TVRSVSAAHAIATYTGPVLLIHGDADRVVPFEHMATLKRAAEKAREDEPDTGDVEVLA
                     IPGGEHSWLYEFAAYRAAIARFLTTAFDGPLPVEDAELIARATPAARLPQREHPFSAI
                     EPEQSGLQTLFRAIRMAGRNRLEPIEPSEATEAT"
     gene            2277..2942
                     /locus_tag="VIF63_03975"
     CDS             2277..2942
                     /locus_tag="VIF63_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8238572.1"
                     /translation="MPKRNPHDVLGVPRTASQATIKAAWRKLARANHPDLTQSDPAAA
                     ERATRRMAEINRAYAELRTAFPVETDGDGTNGRRTWPPEPKPTRPLTGRVDTTATFRP
                     RNTTTGPRIRHDPKPPTTSDRLRREPLRASQPNGPLHRRRMRNFRPPEPPSLEAARAW
                     PIEFGKFHGHTLGQIADFEPTYIDWLARTISRDPDLVAAARVLQDDLDVRGIVRQQRP
                     MPA"
     gene            2939..3547
                     /locus_tag="VIF63_03980"
     CDS             2939..3547
                     /locus_tag="VIF63_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238573.1"
                     /translation="MIRPILASALLALAVAACSPSASPTSTTGDCATDVRAPGTFPDL
                     EALLPRGMIEASPTSVDSGVNCTAEALATYRAHGITALRFAGATWDYGKGNATVVAVL
                     TARPVGQPLLEAAWVEEFYQAGAFAAAHTENIGVTHPNVPGAGIVFRLDTLNNLSLQT
                     VVVFPAETYVHVVIVATEVGPDASREDHDQRVQVAIQVAAGV"
     gene            3588..5264
                     /locus_tag="VIF63_03985"
     CDS             3588..5264
                     /locus_tag="VIF63_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8238574.1"
                     /translation="MSDGTAWGARRQRWMVERLDPALARAPEREKSFRTLGEIELDPL
                     YGPWLERDDERIGMPGEAPFTRGIHPSGYRSRLWTMRMFAGFGAAEDTNSRFKDLLAA
                     GQTGLSIAYDMPTLYGYDTDDEEAEGEFGTCGVGVSSLADMEVLLDGLPLDRVSTSMT
                     INSPAAPIWAMYIVAAEKAGYPRATLEGTTQNDILKEFIAQKEFLFPPDPSMRLVTDT
                     IEFGTKEMPRWNTISISGYHIREAGSTAVQELAFTIADGMAYVEAAIERGLRVDDFAP
                     RLSFFFNSHSDFFEEIAKFRASRRIWHKLMTERYHAENERSTWMRFHTQTAGVSLTPQ
                     QPLNNLTRVAIQALAAVLGGTQSLHTDAYDEALAVPTAEAALLALRQQQVIAEETGVT
                     GTVDPLGGSWFVEALTDEVERQVWVYLDEIDRRGGMVPAVVDGYPQREIAEAAYRFQR
                     EVDERERRIVGVNSYVDMREVTTVPVLQVPQGSLENHMARLERTRRERDATEVEAALR
                     GLREAAARPGSSDSNLMPHFIRCSAAYATLGEQCNVLRGVFGEYREPVGV"
     gene            complement(5272..>5620)
                     /locus_tag="VIF63_03990"
     CDS             complement(5272..>5620)
                     /locus_tag="VIF63_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8238575.1"
                     /translation="PGVADVAPAFFGQLEQLRRHPIVGDIRGKGLMAGIEFVQPGTRT
                     PFPAEVGLTARIDQAARDAGLLVYPCPGIVDGTVGDSILMVPPLVISPAEIGLLVDRL
                     DTALATVTRAVLP"
BASE COUNT          849 a         2020 c         1999 g          752 t
ORIGIN      
        1 cgaccggcac gcgatccgga gccggctccg gcggctcaac gaccttggct tcgcggtcga
       61 cgagatcgag atcgtgccgc agggcacggg ctccgaggtc aacctgcgcg tgaccacgtc
      121 gaaccgtgcg ttccacgccc atgagctcga acgcctgacg gggctcgtgg cgctcgaagg
      181 gcaggcacag ctcctgctca acgacctgcg cgagcacacg gcctggcttc ggctccgcac
      241 ggggcgcacg accgacgaac gggtcggtgc ccgccgctgg cgccgccacg tcctcgaccc
      301 ggcgctgcgc ctgctccggc ccgccgtcgg cgaccgtgac ctcatccagg cgtacagcga
      361 cgttctcgag cacaagtggc tgctgtccga gcgcgccggc cgggacgttg gcctcaaggc
      421 cgcgatcgag gcgtatctcg cggtcggagc gcccgcgccc gaggtaccga cgccggacga
      481 gcccggttcg aaggccacgc tcatggacga ttcgctggag gacgacccgc tggtggacga
      541 acggggcgac tgacctacca gcgctcccgg tgcacgacgc tgtcgagcgg ccgccggcca
      601 cccgcctcgg gcggccgaac gagatccgcc ggctcggcgg ggtagccgaa ggacaggatg
      661 tagccgcagt accggccctc ggggatgccg aggatctcgc tcgcgagctt gggttcgtag
      721 accgtcgccg gacagccgcc gacgccgagc tcccacgccg cgagcagcat gttctcggcc
      781 gcgaggcccg aatcccaggt cagcgagagc gaggcgcccg gcttcgtcgg gtcgggcgtg
      841 acgagcgcga ccgccgcggc agccccggca acgtggccgg catagggccc gaccgtcgac
      901 agccgaacca gggtctcccg cgcctcgatg acgacgaagt cccaggcctg ctggttcttc
      961 gagctgccgg cgtgccggcc ggcgtcgacg atgcgggtga ggtgcgccgg gtcgagcggt
     1021 cgatcctcga agcgccggac catccgcttg gtccggatcg cggtccagac gtccatcagg
     1081 tcgcctcggt cgcttcggac ggttcaatcg gctcgagccg gttccggccg gccatccgga
     1141 tcgcgcggaa gagcgtctgg aggccggact gctcgggctc gatcgccgag aacgggtgct
     1201 ctcgctgcgg cagccgcgcc gccggcgtcg ctcgcgcgat cagctctgcg tcctcgacgg
     1261 gcaggggacc gtcgaacgcc gtcgtgagga accgcgcaat cgcggcgcga taggccgcga
     1321 actcgtagag ccagctgtgc tcgccgcccg ggatcgcgag gacctcgaca tcgccggtgt
     1381 ccggctcgtc ctcgcgggcc ttctcggcgg cgcgtttgag ggtggccatg tgctcgaacg
     1441 gcacgacccg gtcggcatcg ccgtggatca gcagcacggg ccccgtgtag gtggcgatgg
     1501 cgtgggccgc ggacaccgac cgaaccgtgt gcccgcgtgg ctcgaggaat acgtgcgtcg
     1561 tgagccatgc gagcggccac gcgaacggcc cggggatcgg cagcttcgcg aggcggaagg
     1621 tctggcgcgt gagccggtag ggatcggccg gtgtcgaggc ggcgaccacg gccgcaacgc
     1681 gcggttcggc cgcagcggcg agcagcgcac cgatcccgcc catcgaatgg ccgaacacgc
     1741 cgacggccgt cacgtccttc cgagccagca gcgcccggac gccggccaga gcgtcgagcc
     1801 cgaactcacc cgccgtcagc ggcagctcct cgggcgggtt ctcgccgtgg gcgcggatgt
     1861 cgatcgtcag gacgtggatc ccgatcgcgt tgaggatcag cgcgttcggg agcgtccggt
     1921 cgcgcgccga ctcccagccg tggacgagca gcaccgcggg tcctggcttg ccgccgcgcg
     1981 ccgggatcca ccatgccggc aggtcgaggt cgccagaggg cacggtgagc gactcgaacg
     2041 ccagcccgag gaagctcggg tcgtgatccg gcgggtgccg gtgcggatag ccggcgcggg
     2101 cgcggtacgc ctgcgcgaat cgccaggccg cgatcgggcc gacggtcagc gcagcggtca
     2161 gtccgagcct gcgacgcaac atccgggaaa tggtaggtcg cgggcggggg agcagcgggc
     2221 gacgccgctc ggtgcgccgg agcaggccgg cgactcgccc tcgatacact tctgccgtgc
     2281 ccaaacgcaa tccccacgac gtgctcggcg tgccccggac cgcgagccag gccacgatca
     2341 aggcggcctg gcgcaagctc gcgcgcgcca atcacccgga cctcacgcag tccgaccctg
     2401 ccgcggccga acgcgcgact cggcgcatgg ccgagatcaa ccgtgcctac gccgagctgc
     2461 gcacggcatt tcccgtcgag acggacggtg atgggacgaa cggccgccgg acgtggccac
     2521 ccgagccgaa gccgacgcgt ccgctgaccg gccgggtcga cacgaccgcg accttccggc
     2581 cgcgcaacac gacgaccggc ccgcggatcc gccacgaccc aaagccgccg acgacctccg
     2641 accgactgcg tcgcgagccg ctccgggcct cgcaaccgaa cggcccgctc catcgccggc
     2701 gcatgcgcaa cttccgaccg cccgagccgc catcgcttga ggcggcccgc gcctggccga
     2761 tcgagttcgg caagttccac ggccacaccc tcggtcagat cgcggacttc gagccgacct
     2821 acatcgattg gctggcgcgg acgatctccc gtgatcccga cctcgtcgcc gccgcccgcg
     2881 tcctccagga cgacctcgac gtccggggca tcgtccgcca gcaacggccc atgcccgcgt
     2941 gatccgcccg atccttgcga gcgcgctgct cgccctggcc gtcgcggcct gctccccgag
     3001 cgcgagcccg acatcgacga cgggcgactg cgcgaccgac gtccgcgccc cgggcacctt
     3061 ccccgacctc gaggcgctgc tgccgcgggg catgatcgag gcgtcgccca cctcggtcga
     3121 ctcgggcgtg aactgcacgg cagaggcact cgcgacctac cgggcgcacg gcatcacggc
     3181 gcttcgattc gctggcgcga cctgggacta cggcaagggc aatgcgacgg tcgtggccgt
     3241 gctcacagcg cggccggtcg gccagccgct gctcgaagcg gcgtgggtcg aggagttcta
     3301 ccaggcgggc gccttcgccg ccgcccacac cgagaacatc ggggtcaccc acccgaacgt
     3361 gcccggcgcc ggcatcgtct tccgcctgga cacgctcaac aacctctcgc tccagacggt
     3421 cgtcgtgttc ccggccgaga cctacgtgca cgtcgtcatc gttgcgaccg aggtcgggcc
     3481 cgatgcgtca cgtgaggacc acgaccagcg cgtgcaggtc gcgatccagg tcgcggcggg
     3541 ggtttgacct cggggcgtcg tccggagccc gtggtagtgt cggccgcatg agcgacggga
     3601 ccgcctgggg ggcacgccgg cagcgctgga tggtcgagcg gctcgacccg gccctcgctc
     3661 gggcgcccga gcgcgagaag agcttccgaa ccctcggcga gatcgagctc gacccgctgt
     3721 acgggccctg gctcgagcgc gacgacgagc gcatcggaat gcccggtgag gcgccgttca
     3781 cgcggggcat ccacccgtcc ggctatcgat cgcgcctgtg gaccatgcgc atgttcgcgg
     3841 gcttcggggc cgcggaggac acgaactcaa ggttcaagga cctgctcgcg gcgggccaga
     3901 cgggcctgtc gatcgcctac gacatgccga cgctctacgg ctacgacacc gacgacgagg
     3961 aggccgaggg cgagttcggg acgtgcggtg tcggggtctc gtcgctggcc gacatggagg
     4021 tcctcctcga cggcctgccg ctcgatcgcg tctcgacgtc gatgacgatc aactcgcccg
     4081 ccgccccgat ctgggcgatg tacatcgtgg ccgccgagaa ggcgggctac ccgcgagcga
     4141 cgctcgaggg cacgacccag aacgacatcc tcaaggaatt catcgcccag aaggagttcc
     4201 tgttcccacc cgacccatcg atgcgcctcg tgacggacac gatcgagttc ggcacgaagg
     4261 agatgccccg ctggaacacg atctcgatct cgggctacca catccgcgag gccggttcga
     4321 ccgcagtcca ggagctggcg ttcacgatcg ccgacggcat ggcctacgtc gaggcggcga
     4381 tcgagcgcgg tttgcgcgtc gacgacttcg cgccgcgcct ctcgttcttc ttcaactcgc
     4441 acagcgactt cttcgaggag atcgccaagt tccgggcgtc gcgccgaatc tggcacaagc
     4501 tcatgaccga gcgctatcac gcggagaacg agcgctcgac atggatgcgg ttccacaccc
     4561 agaccgccgg cgtctccctg acgccgcagc agccgctcaa caacctgact cgcgtcgcga
     4621 tccaggcgct cgccgcggtc ctcggcggga cgcagtcact gcataccgat gcatatgacg
     4681 aggctctggc ggtgccgacg gcggaggcgg cgctgctggc gctgcgccag cagcaggtga
     4741 tcgccgagga gacgggcgtg accggcacgg tcgatccgct cggcggctcg tggttcgtcg
     4801 aggcgctgac cgacgaggtc gagcgccagg tctgggtcta cctcgacgag atcgaccggc
     4861 gcgggggcat ggtgcccgct gtcgtcgacg gctacccgca gcgcgagatc gcggaggccg
     4921 cgtaccgatt ccagcgtgaa gtcgacgagc gcgagcgccg gatcgtcggc gtcaactcgt
     4981 acgtggacat gcgcgaggtc acgaccgtgc cggtgctcca ggtcccacag ggctccctgg
     5041 agaaccacat ggcccgcctg gagcggacgc gtcgggagcg ggacgccacg gaggtcgagg
     5101 cggcactgcg cgggctgcgc gaggcggccg ctcgcccggg ctcgtctgac tcgaacctga
     5161 tgccgcactt catccgctgc tctgcggcgt acgcgacgct cggcgagcag tgcaacgtcc
     5221 tgcggggcgt cttcggcgag taccgcgagc ccgtcggggt ctagcgcggg gctagggcag
     5281 gaccgctcgg gtcacggtcg cgagtgcggt gtcgagccga tcgacgagca gcccgatctc
     5341 cgccggcgag atcacgagcg gcgggaccat gaggatcgaa tcgcccacgg ttccgtcgac
     5401 gatgcccggg caggggtaga ccagcaggcc ggcgtcgcgc gccgcctggt cgatccgggc
     5461 agtgagcccg acctcggccg ggaacggcgt ccgggtgccc ggctggacga actcgatccc
     5521 ggccatgagg cccttgcccc ggatgtcgcc gacgatcgga tggcggcgga gctgctcgag
     5581 ctggccgaag aaggccggcg ccacgtctgc gacgccgggc
//
LOCUS       DATBSW010000145         7720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_435488, whole genome shotgun sequence.
ACCESSION   DATBSW010000145 DATBSW010000000
VERSION     DATBSW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435488"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VIF63_03995"
     CDS             complement(<1..933)
                     /locus_tag="VIF63_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8238576.1"
                     /translation="MERRRARPRIADVAREAGVSKTAVSFAFNSPERLSAETAIRIRE
                     VADTLGYRPNPVARMLTQRQTMTLGVLTPQALAVIFSNPFFALFSEGVAHAAEDLGYE
                     LHFISPLHGSLALAVGRSTVDGVVAIGLSADHPEVGQIRGAGLPMVLVDSDDLPEHSS
                     VVVDDEAGARAAAEHLIALGHRDILVIAVEGPAAASGEITRNGVSDRRLRGYRTALGA
                     AGINLDADRVVAGRASIDGGSSAFQRAWAMGARPTAILAMSDAMAIGAMRAARELGMH
                     IPDDLSVVGFDDIDLAGHVDPPLTTVHQPIRQKGA"
     gene            1101..1469
                     /locus_tag="VIF63_04000"
     CDS             1101..1469
                     /locus_tag="VIF63_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238577.1"
                     /translation="MPSIRGMPWGVALFLAYGALILIGVGLSLGSVVDQAIIVPITLQ
                     GAVLMALLAYTIFTMTLVLQRKAAARGLALGLTTLAVPAVPLAVLNGQVIGALFIVVV
                     ALLLVRGLRAPSALAWLDQE"
     gene            1549..2484
                     /locus_tag="VIF63_04005"
     CDS             1549..2484
                     /locus_tag="VIF63_04005"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY8238578.1"
                     /translation="MTTPPRRPSPASRATRRSRRPAARSESFLQRNRSRLLWAGGILV
                     FALLVFVGFVLPAVTPAYSCDNEFNPTPAPPWVAPSPAPVASGATPAPAVTAPPPGYV
                     QPDMGHVHEANTGKPIDYLYCPPASGKHYPAPGGPIAAKLYGPDERTTPGGWIHNLEH
                     GAIVVLYNCALAPDACTDTGQQAFTTMLAHWPKSPICNIPGIDPQKQTPVITRFEDMP
                     YPYAAIVWDVVLPLQTLDEASIFAFYAGQAERFNPEPRCPAPTPTPGPTPTAGPTPSA
                     GPTSSAPASGAPSVSPSVAPSVAPAASNTTAPAGS"
     gene            2501..3529
                     /gene="tdh"
                     /locus_tag="VIF63_04010"
     CDS             2501..3529
                     /gene="tdh"
                     /locus_tag="VIF63_04010"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY8238579.1"
                     /translation="MLALVKADAGPGLRLQEVPRPEIGINDVLIRVHKTGICGTDLHI
                     ESWDPWAAKTIHPPLVVGHEFVGEVVEVGSNVNDFHPGDLVSGEGHVTCGRCRHCMAG
                     QRHLCANTLGLGVNRDGAFAEYVALPMTNIWHHWAGIDEEVAAIFDPFGNAVHTALAF
                     EVHGEDVLISGAGPIGLMAIPVVRHAGARHVVISEPNAFRRELATQMGATIAVDPRER
                     DLKDVQRELGMVEGFDVALEMSGNAVALQSAISNMAHGGRIAILGIPTGEISIDVNKV
                     VFDQLTLRGIYGRQMYESWYQMAVLVSSGLDIKPVITHRFDVKEFEAAFAAARSGDSG
                     KVIMDWGA"
     gene            3529..4746
                     /locus_tag="VIF63_04015"
     CDS             3529..4746
                     /locus_tag="VIF63_04015"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY8238580.1"
                     /translation="MTRTRDPLAFLGTELDELKARHLYRPLRVMSAAQGPVTVVDGRE
                     VISLSSNDYLGLTHHPRLRDAALKAIGEFGVGSGAVRTIAGTMTEHETLEAELAEFKG
                     VEAVLTFQSGFTANTGVIPTITGEQDLIVSDALNHASIIDGMRLSKAPRKVFPHRDVA
                     ALREILQEAAANGRPDGSGPYRLILVVTDGVFSMDGDIAPLPGIVEAAEEVGAAVFVD
                     DAHASGVLGRDGRGSVDHFGLHGRVDIQVGTMSKAMGVLGGYVAGSLALREMLVQRAR
                     PFLFSTSHPPADAAACREAIRVMQEEPWRIERLWASTKRFKAELTRLGFDTGRSETPI
                     TPIIVGEADDAIRFSSRMFEEGVFATSVVFPTVAIDQARLRTIVTAALTDEHLDRALA
                     AFSKVGRELAIIG"
     gene            4857..5747
                     /locus_tag="VIF63_04020"
     CDS             4857..5747
                     /locus_tag="VIF63_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEY8238581.1"
                     /translation="MVAEPPRGLEPSRNLPLDAHLHTDQSPDSSVPIDVYAALAVERG
                     IAEIAITDHVDFDPRDPAFEYSRYDDRERVVRGAAERWAREGVVIRFGAELTYNQRWD
                     ADVRAHLKRYRYDYVIGSVHDWPESPYWPSRIRGWVEGRSLDEVVAPYYAEIIAAARS
                     GLFDTIGHLDVVKRYLHPSITAADLATRPDLQEPALRAIIESGVSLEVNSSGLRYPGA
                     ETYPSAAVVGRYRELGGERVVVGSDAHSRGSFGARLDESYRHLVAAGFDALSFRRGAE
                     RVRIEVPRGTSDSTVDSLHP"
     gene            5774..6385
                     /locus_tag="VIF63_04025"
     CDS             5774..6385
                     /locus_tag="VIF63_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8238582.1"
                     /translation="MDDETLDRLVEKAKSGDREAFGRIFDAYGAPIYRFIASRVNRPS
                     DAEDLTQLVFVKALEALPRYEARGIPFGGWLFRLARNAIIDQMRTKRDHLSLVAAVTR
                     ETEEAGPEAQASLRDDLDAVARAMSELTDDQREVIELRFFAGLSVEETADAMGRQEGT
                     VRGLQFRAIASLRRALGIEVPALAPAGSRRAAAVAAMTLPIIR"
     gene            6382..7161
                     /locus_tag="VIF63_04030"
     CDS             6382..7161
                     /locus_tag="VIF63_04030"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEY8238583.1"
                     /translation="MSSYSDRELLALDQMEDLLEAYADARLSPTGPVLARMRAQVLAQ
                     AATMASIHAAARRVLDTGEARAPRWSAPRLTVPRLMVPRLTVPRRAFSVVGAGLLTVA
                     MTTAVLGAPAGSPFYNARVAVEAAFLPSQPDARLAAQEAHLAERLAEAEAAAAKGDVV
                     ALDAALAAYKVEVEAAVSDVVDSADKVAKFETVLAKHVATLEALGARLTNEQASQNAV
                     DHAAQASERAIEKLKAKADQGNQGGGKPAPKPPNPNKPDRE"
     gene            complement(7214..>7720)
                     /locus_tag="VIF63_04035"
     CDS             complement(7214..>7720)
                     /locus_tag="VIF63_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY8238584.1"
                     /translation="LSLVWFANRIRRRRAEGLSTHDLAFGTAVGLGLHNLSEGLAIGQ
                     SAATGATAFALILVAGFALHNATEGFGIAAPLTSGPRPSWRFLASLGLIGGGPTFLGG
                     VIGYSFVSPLLSILFLGLAAGALIYVFNDMMAVSRKFAEPLASNVALIVGLLVAFGTD
                     FLLEAVGT"
BASE COUNT         1183 a         2747 c         2673 g         1117 t
ORIGIN      
        1 ggcgcccttc tgacggatcg gctggtggac cgtcgtcaga ggcgggtcga cgtggccggc
       61 gaggtcgatg tcgtcgaagc cgacgacgct gaggtcgtcg gggatgtgca tgcccagctc
      121 acgcgcggcg cgcatggcgc cgatcgccat cgcgtcactc atggccagga tggccgtcgg
      181 gcgggcgccc atcgcccacg cgcgctggaa ggcggacgag ccgccgtcga tggaggctcg
      241 accggcgacg acgcgatctg cgtcgaggtt gatgcccgcg gcgccgagcg ccgttcgata
      301 gccgcgcagc cggcggtccg acacgccgtt ccgggtgatc tcgccggagg ccgccgccgg
      361 gccctcgacg gcgatcacga ggatgtcgcg atgaccgagg gcgatgaggt gctcggccgc
      421 ggctcgcgcg ccggcctcgt cgtcgacgac gaccgagctg tgctcgggca ggtcatcgga
      481 gtcgacgagc accatcggca gcccggcgcc gcggatctgg ccgacctcgg ggtggtcggc
      541 cgagaggccg atcgccacga cgccgtcgac cgtcgaccgg ccgaccgcga gggccagcga
      601 gccgtgcagc ggcgagatga aatggagctc gtaaccgagg tcctcggcgg cgtgggcgac
      661 gccctcgctg aagagcgcga agaacgggtt cgagaagatg accgcgaggg cctgcggggt
      721 gagcacgccg agggtcatcg tctggcgctg ggtgagcatg cgcgcgacgg gattcggccg
      781 gtagccgagc gtgtcggcga cctcgcgaat ccggattgcc gtctccgcgg acagccgctc
      841 cgggctgttg aacgcgaagg acaccgccgt cttcgacacg cccgcttcac gggcgacgtc
      901 agcgatgcgc gggcgtgctc tgcgccgctc cacagtcctc ctccagcgac gggggtgagc
      961 tccgtcgcgc cgctgcgagc cgtcgtcggg tcttctcggc ctcgcaggtg aaccggttta
     1021 gtgaaccggt ttagtgacag tagcgatccg gttcgcggcc gtcaagagtc cggcgctgcc
     1081 cgacgcccca caatgggccg atgccttcga ttcgcggcat gccctggggc gtggcgctct
     1141 tcctcgccta cggggcgctg atcctgatcg gcgtgggcct gtcgcttgga tccgtcgtgg
     1201 accaggcgat catcgtcccg atcacgctcc agggcgccgt gctcatggcc ctgctcgcct
     1261 acacgatctt cacgatgacg ctcgtcctcc agcgcaaggc cgccgcgcgc ggcctcgccc
     1321 tcggcctgac tacgctggcc gtgcccgcgg tgccgctggc ggtgctcaac ggccaggtca
     1381 tcggggcgct gttcatcgtc gtcgtggcac tgctcctcgt ccggggcctg cgcgcgccga
     1441 gtgcgctggc gtggctggac caggagtgag cctctccgcc cgcgtccgga cgcccgatct
     1501 ggcagacttc ctcgccgatc cccccgccgc tccagtgagg ccgaccactt gaccacgccc
     1561 ccgcgccgcc cgagtcccgc cagccgggcc acgcggcgct cgcgccgacc cgctgcccgc
     1621 agcgagagct tcctccagcg caaccgcagc cggttgctgt gggcgggcgg catcctcgtc
     1681 ttcgccctgc tcgtcttcgt cggcttcgtg ctgccggcag tgacgcccgc gtactcctgc
     1741 gacaacgagt tcaacccgac gcccgccccg ccatgggtgg caccaagccc ggcgcccgtt
     1801 gcgtccggtg cgacgccggc acccgcggtg accgctccgc cgcccggcta cgtccagccg
     1861 gacatgggcc atgtccacga ggccaacacg ggcaagccga tcgattacct gtactgcccg
     1921 ccggcaagcg ggaagcacta cccggcgccc ggaggtccca tcgccgcgaa gctgtacggc
     1981 cccgatgagc ggacgacgcc cggtggctgg atccacaacc tcgagcacgg cgcgatcgtg
     2041 gtcctgtaca actgcgcact ggctcctgac gcctgcaccg acaccggcca acaggcattc
     2101 acgacgatgc tcgcgcattg gccgaagagt ccgatctgca acatcccggg catcgatccc
     2161 caaaagcaga cgccggtcat cacgcgcttc gaggacatgc cctacccgta cgccgccatc
     2221 gtgtgggacg ttgtcctgcc gctccagacc ctcgacgagg cctcgatctt cgcgttctac
     2281 gcgggacagg ccgagcgctt caaccccgaa ccgcgctgtc cggctccgac cccgacgccc
     2341 gggccgacgc cgaccgccgg gccgacgccg agcgccggcc cgaccagcag cgcgcccgcc
     2401 tcgggcgctc cgtcggtctc tccatcggtc gcgccgtcag tcgccccggc agcctcgaac
     2461 accacagcgc cggccggcag ctagcggaga cgccaatcag atgctcgccc tggtcaaagc
     2521 cgacgcgggg cccgggctgc ggctccagga ggtgccgcgg ccggagatcg gcatcaacga
     2581 cgtcctgatc cgggtccaca agaccgggat ctgcggcacc gacctgcaca tcgagtcatg
     2641 ggatccgtgg gcggcgaaga cgatccaccc gccgctcgtc gtcggccatg agttcgtcgg
     2701 cgaggtcgtc gaggtcggaa gcaacgtcaa cgacttccac ccgggcgatc tcgtcagcgg
     2761 cgagggccac gtgacctgcg gtcggtgccg gcactgcatg gccggccagc gccacctgtg
     2821 cgcgaacacg cttggcctcg gcgtgaaccg tgacggcgcc ttcgccgagt acgtcgccct
     2881 gccgatgacc aacatctggc accactgggc cgggatcgac gaggaggtcg ccgcgatctt
     2941 cgacccgttc ggcaacgccg tgcacacggc gctcgcgttc gaggtgcacg gcgaggacgt
     3001 gctcatctcc ggcgcgggcc cgatcgggct catggccatt cccgtcgtgc gccacgccgg
     3061 cgcccgccac gtcgtgatca gcgagccgaa cgccttccgc cgcgagctcg ccacgcagat
     3121 gggcgccacg atcgccgtcg acccacgcga gcgcgacctc aaggacgtcc agcgcgagct
     3181 gggcatggtc gagggcttcg acgtcgccct cgagatgtcg ggcaatgcgg tggcgctcca
     3241 gtcagccatc tcgaacatgg cccacggcgg ccggatcgcg atcctcggca tcccgacggg
     3301 cgagatcagc atcgatgtca acaaggtggt cttcgaccag ctgaccctgc gcggcatcta
     3361 cggccggcaa atgtacgaga gctggtacca gatggcggtg ctggtgagct cgggcctcga
     3421 catcaagccc gtcatcaccc atcgcttcga cgtcaaggag ttcgaggcgg cgttcgccgc
     3481 ggcccgctcg ggcgattcgg gcaaggtgat catggattgg ggcgcctgat gacacgaaca
     3541 cgagacccgc tcgccttcct cggcacggag ctcgacgagc tcaaggccag gcacctgtac
     3601 cggccgttgc gggtcatgag cgcggcccag ggccctgtca cggtcgtcga cggccgcgag
     3661 gtgatctcgc tctcctcgaa cgactacctc gggctcaccc accacccccg gctgcgcgac
     3721 gccgcgctca aggcgatcgg cgagttcggg gtcgggtccg gcgccgtccg cacgatcgcc
     3781 ggcacgatga ccgagcacga gacgctcgag gccgagctgg ccgagttcaa gggcgtcgag
     3841 gcggtgctga cgttccagtc gggcttcacc gccaatacgg gcgtgatccc gacgatcacc
     3901 ggcgagcagg acctgatcgt gtccgacgcg ctcaaccacg cctcgatcat cgacggcatg
     3961 cgcctgtcga aggcgccgcg caaggtgttc ccgcatcgcg acgtcgcggc actgcgcgag
     4021 atcctgcagg aggcggcggc gaacggccgc ccggacgggt cgggaccgta ccggctgatc
     4081 ctcgtcgtga ccgacggcgt gttctcgatg gacggcgata tcgcgccgct gcccggcatc
     4141 gtcgaggcgg ccgaggaggt cggcgcggcg gtgttcgtcg acgacgcgca tgcctcgggc
     4201 gtgctcggtc gcgacgggcg cggctcggtg gaccacttcg ggctgcacgg ccgggtcgac
     4261 atccaggtcg gcacgatgtc gaaggcgatg ggcgtgctcg gcggctacgt cgcgggctcg
     4321 ctggcactgc gcgagatgct cgtccagcgc gcccggccgt tcctcttctc gacctcgcac
     4381 ccaccggccg atgccgcggc gtgccgcgag gcgatccggg tgatgcagga agagccgtgg
     4441 cggattgagc ggctgtgggc aagcacgaag cgcttcaagg ccgagctgac ccgcctcggc
     4501 ttcgacaccg ggcggtccga gacgccgatc accccgatca tcgtcggcga ggccgacgac
     4561 gccatccgct tctcgtcacg gatgttcgag gagggcgtct tcgccacctc ggtcgtcttc
     4621 ccgaccgttg cgatcgacca ggcgcgactg cgcacgatcg tcaccgcggc cctgaccgac
     4681 gaacatctcg accgcgcgct cgccgcgttc tcgaaggttg gccgagagct ggcgatcatt
     4741 ggctgaagga gtcgcgtccc ggccgtccga ggtcgaaggc ggagcgccgc gacgccattc
     4801 gcccgaggat gctcggcctg atctcgggct gagctcaccg tgatgcgcag ctgatcgtgg
     4861 tcgccgaacc gccgaggggc ctcgagccgt cgcggaacct gccgctcgac gcccatctcc
     4921 acacggatca gtcgcccgac agctcggtcc ccatcgacgt ctacgcggcc ctcgcggtcg
     4981 agcgcgggat cgccgagatc gcgatcaccg accacgtcga cttcgatccc cgcgaccccg
     5041 ccttcgagta ctcgcgctac gacgaccgcg agcgcgtcgt tcgtggcgcc gccgagcgct
     5101 gggcgcgcga gggcgtcgtg attcgcttcg gggcggagct cacgtacaac cagcgctggg
     5161 acgcggacgt tcgcgcgcac ctcaagcgct accgctacga ctacgtgatc ggatcggtcc
     5221 atgactggcc cgagtcgccg tactggccgt cgcgcatccg gggctgggtc gagggtcggt
     5281 cgctcgacga ggtcgtcgcg ccgtactacg ccgagatcat cgccgcggcg cgatccggcc
     5341 tgttcgacac catcggccac ctcgacgtcg tcaagcgcta cctgcacccg tccatcacgg
     5401 cggccgacct ggccactcgt ccggacctgc aggagcccgc cctgcgggcg atcatcgagt
     5461 ccggggtgtc gctcgaagtc aacagcagtg ggttgagata tcccggggca gagacgtatc
     5521 cttcggcggc cgtggtgggg cggtatcgcg agctgggggg agaacgggtg gtcgtggggt
     5581 cggatgccca ctcgagggga tcattcggag ctcgactgga cgagtcgtat cggcacctgg
     5641 tcgccgcggg attcgacgcc ctgagcttcc gccgcggagc cgaacgcgta cggatcgaag
     5701 tgccacgtgg cacctccgac agtaccgtcg actcgttgca cccataacgc cgagtactga
     5761 tcctcgttac ccaatggacg acgaaacgct ggatcggctc gttgagaagg ccaaatcggg
     5821 cgaccgagag gccttcggca ggatcttcga tgcgtatggc gcgccgatct accgcttcat
     5881 cgccagccgc gtcaatcgcc cgagcgacgc ggaggacctg acgcagctcg tcttcgtcaa
     5941 ggccctggaa gcgctcccgc gctacgaggc gcggggcatc ccgttcgggg gctggctctt
     6001 ccggctggcc aggaacgcga tcatcgacca gatgcgaacg aaacgggacc atctctcgct
     6061 cgtggccgcc gtgactcggg agaccgagga ggcggggccc gaggcccagg cgtccctccg
     6121 ggacgacctc gatgcggtgg cccgggccat gtcggagctg accgacgatc aacgtgaggt
     6181 catcgagctg cgcttctttg ctgggctctc ggttgaggaa acggcggatg cgatgggtcg
     6241 ccaggaaggc accgtccgcg ggctgcaatt ccgcgccatc gcgtcccttc gacgcgcgct
     6301 cgggatcgag gtccctgcgc tggctcccgc aggcagtcgc agggccgcgg cggtcgcggc
     6361 catgaccctg ccgatcatcc gatgagcagc tactcggacc gcgagctcct cgccctcgac
     6421 cagatggagg atctgctcga ggcgtatgcc gacgctcgcc tctccccgac cggccccgtg
     6481 ctggcccgca tgcgggcgca ggtcctcgcc caggccgcca cgatggcctc gatccacgcc
     6541 gctgcgcgcc gggtcctcga cacgggcgag gcgagggcgc cgcgctggtc agcgccgcga
     6601 ctcacggtgc cgcgactcat ggtgccgcga ctcacggtgc cgcggcgcgc gttcagcgtc
     6661 gtcggcgccg gcctgttgac ggtcgcgatg acgacggccg tcctcggcgc cccggccggc
     6721 agcccgttct acaacgcccg tgtcgcggtc gaggcggcgt tcctgccgtc gcagcccgat
     6781 gcccgcctcg cggcccagga agcgcacctc gcggaacgcc tggccgaggc agaggccgcc
     6841 gcggcgaagg gcgatgtcgt ggcgctcgat gcggccctcg cggcctataa ggtcgaggtc
     6901 gaagcggccg tctccgacgt cgtggacagc gccgacaagg tggcgaagtt cgagacggtg
     6961 ctcgcgaagc acgtcgccac gctcgaggcc ctcggcgccc ggctcaccaa tgagcaggcg
     7021 agccagaacg cggtcgacca cgccgcacag gcgagcgagc gcgcgatcga gaagctcaag
     7081 gcaaaggccg accagggcaa tcagggcggc ggcaagccgg cgcccaagcc gccgaacccg
     7141 aacaagccgg accgcgagta accgtacccc tctcgagact cggaaacatg gccgccgctc
     7201 ggcggccacc gcctcaggtg ccgaccgcct cgagcaggaa gtccgtcccg aacgcgacaa
     7261 ggagcccgac gatgagggct acgttcgacg cgagtggctc tgcgaacttc cgggacacgg
     7321 ccatcatgtc gttgaagacg tagatgagtg cgcccgcggc gaggcccagg aagaggatcg
     7381 acaggagcgg cgacacgaac gagtagccga tgacgccgcc gaggaacgtc gggccaccgc
     7441 cgatgaggcc gaggctggcc aggaaccgcc acgaaggtcg cgggccgctc gtgagcggag
     7501 cggcgatgcc gaatccctcg gtcgcattgt ggagcgcgaa cccggcgacg aggatcaggg
     7561 cgaaggcagt ggcgccggtg gcagccgatt gcccgattgc aaggccctcc gagagattgt
     7621 gcagcccgag tcccacggca gttccgaacg caaggtcgtg ggtcgagagg ccctctgccc
     7681 gccgccgccg gatccggttc gcaaaccaga cgagtgagag
//
LOCUS       DATBSW010000146         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_117824, whole genome shotgun sequence.
ACCESSION   DATBSW010000146 DATBSW010000000
VERSION     DATBSW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117824"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VIF63_04040"
     CDS             <1..258
                     /locus_tag="VIF63_04040"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238585.1"
                     /translation="VLKRRLDVLDGIAFGWPELAREVAEGDVIDIVAQLRSRRFGGIE
                     SLQLEVRDAAPSGFHPEARLILEAVPVAVGPGPAIAPAPAA"
     gene            266..1516
                     /locus_tag="VIF63_04045"
     CDS             266..1516
                     /locus_tag="VIF63_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238586.1"
                     /translation="MTRANPRPRAPAPAGGGRLPFDIAPAASVLGLIVVATVSFALLG
                     GSLPTIPGNGGPDGPIRTATPSNVVIVDPRADIPGTLLYVKAGDAGPDGNVWAQSGDH
                     ARQLTTGSADAMPAWSPDGTSIYFIRSARETGRWPSSGVTRTYNLSVPSLVRMNADGA
                     GEPDVVLVGRLKRGSNTWSYFIREPSISPDGTTAAIISDGPDPTTSDIVVKLLDLASG
                     KLTNPTLGQTQSLGHQDPAWSPDGKVLLYVRNAREGARGTPAIYRYTLATDKSTALTG
                     PGYTAPAWSRDGRFVAATKTSNFGTDVVILDGKTGAELLRVTRDEQSFSPVWSPLMDS
                     VAFFRVEHGVVDLYLVPLTGTGPDWTAGEPIALTISAGLDAASRPAWFVPADQLPPLP
                     TPTPAITPAPSGSAPSGGPGSSNP"
     gene            1513..2400
                     /gene="pyrF"
                     /locus_tag="VIF63_04050"
     CDS             1513..2400
                     /gene="pyrF"
                     /locus_tag="VIF63_04050"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8238587.1"
                     /translation="MSGTGTTYLERLSARSAATGTVLCLGIDPDPALLPPGFADNIAD
                     IERFAMLILDAALPFAAAVKPNLAFFEAYGAAGWAALERVRARIPADVPMIADAKRGD
                     IASTSARQVVGYFDSLGVDAVTISPYLGQDSIAGYLERPDRFAYVLCRTSNPGAADIQ
                     GLRVRARWDGEGVMTGPDEPLWARVARDASSWGPGGTVGLVVGATAPEELRAARSIAP
                     GLAFLVPGVGAQGGGIEPVLAAGPATAAPAGGRPGGGLVVNVSRAIAAAALEGPPAEL
                     ESRLAEAARSWSAKLPVLP"
     gene            2446..2787
                     /locus_tag="VIF63_04055"
     CDS             2446..2787
                     /locus_tag="VIF63_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8238588.1"
                     /translation="MPFNIGPGELIIVLVIALVVLGPGKLPDVAASLGKSLREFRKAA
                     TDVSDATKIDAPLPSPPVAAVTPPAPMPAPVAPPAVAVNPADPADAGAPADAGAPAAT
                     GAEPARDPNVP"
     gene            2824..3564
                     /gene="tatC"
                     /locus_tag="VIF63_04060"
     CDS             2824..3564
                     /gene="tatC"
                     /locus_tag="VIF63_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8238589.1"
                     /translation="MTTPITASDAAEETQQAQMPLSGHLAELRNRLIWSILAIGLGSM
                     VGFYLGDELIAILRAPLPPNVPLILLDVTGAFSIRLQVSLVVGVVLAMPVILFHVWRF
                     VAPGLTASERRSVLPWIPISLLFFLLGVSIAYIIMPFAVTFLLSFVLEGVDPTIQLGS
                     YFSFVTSLFLAFGILMEFPILLIGLSRVNIVTSELLSRSRRMVILVIALFSAVATPGG
                     DLVSPTILGVTLYILFEATVLFIRRTGR"
     gene            3586..>3959
                     /locus_tag="VIF63_04065"
     CDS             3586..>3959
                     /locus_tag="VIF63_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY8238590.1"
                     /translation="MATEPTAEGNAPSGPHVVVLTGLSGGGKTAAAKLFEDLAYTVVD
                     NLPGELLPNLAELVVQDPARFARVAIVLDVRAGDAETAYRQMRGALEGRGIRPQVFFL
                     EANDETLIRRFSETRHRHPLAIG"
BASE COUNT          504 a         1520 c         1350 g          585 t
ORIGIN      
        1 gtgctgaagc gccgcctcga cgtgctcgac gggatcgcct tcggctggcc ggagctcgcc
       61 cgcgaggtcg ccgagggcga cgtgatcgac atcgtcgccc agctccggag ccgccgcttc
      121 ggcggcatcg agtcgctcca gctcgaggta cgcgacgccg ctccatccgg gttccacccc
      181 gaagcccgcc tgatcctcga ggcggtgccg gtcgccgtgg ggcctgggcc cgcgatcgct
      241 ccggcgccgg cggcctgacc ccgcgatgac ccgcgccaat ccgcgtccgc gcgcccctgc
      301 gccggccggc ggcggccgcc tcccgttcga catcgcgccg gccgcgtcgg tcctgggcct
      361 gatcgtcgtg gcgacggtca gcttcgccct gctcggcggc tcgctgccga cgatcccagg
      421 caacggcggg ccggatggcc cgatccgcac ggccaccccg tcgaacgtcg tgatcgtcga
      481 tccgcgggcc gacatccccg gcacgctcct ctacgtgaag gccggcgacg ctggcccgga
      541 cggcaacgtg tgggcccagt ccggcgacca cgcgcggcag ctgacgaccg gcagcgccga
      601 cgcgatgccg gcgtggtccc ccgacggcac gtcgatctac ttcatccgct ccgcgcgcga
      661 gaccgggcgg tggccctcct cgggcgtcac ccgcacgtac aacctgtcgg tcccgagcct
      721 cgtccgtatg aacgcggacg gcgccggcga gccggacgtc gtcctcgtcg gccggctgaa
      781 gcgcggctcg aacacgtggt cgtacttcat ccgcgagcct tcgatctcgc ccgacggcac
      841 gaccgcggcg atcatcagcg acggccccga cccgacgacg agcgacatcg tggtcaagct
      901 cctggatctc gcaagcggca agctgacgaa cccgacgctc ggccagacgc agtcgctcgg
      961 gcatcaggac ccggcctggt cgccggatgg caaggtcctc ctgtacgtgc ggaacgcgcg
     1021 cgagggcgca cgcggcacgc ccgcgatcta ccggtacacc ctcgcgacgg acaagtcgac
     1081 ggcgctgacc ggacccggct acaccgcgcc ggcgtggtcg cgcgacgggc ggttcgtggc
     1141 cgcgacgaag accagcaact tcggcacgga cgtggtcatc ctcgacggca agacgggcgc
     1201 ggagctcctc cgcgtcaccc gcgacgagca gtcgttcagc ccggtatggt cgccgctgat
     1261 ggactcggtg gcgttcttcc gcgtcgagca cggggtcgtg gacctgtacc tcgtcccgct
     1321 gaccggcacg ggccccgact ggacggcggg cgagccgatt gcgctgacga tctccgccgg
     1381 gctcgatgcg gcgtcgcggc ccgcctggtt cgtccccgcc gatcagttgc cgccgctgcc
     1441 gacgccaaca cccgcgatca cgccggcgcc gtccggaagt gcgccgtccg gcggtcccgg
     1501 atcgtcgaac ccatgagcgg gacgggcacc acgtacctcg agcggctttc ggcccggagc
     1561 gcggccacgg gcacggtgct gtgcctcggg attgaccccg atccggcact gctgccgccg
     1621 ggatttgccg acaacatcgc ggacatcgag cggttcgcga tgctcatcct cgacgcggcc
     1681 ctcccgttcg cggccgcggt caaaccgaat ctcgccttct tcgaggccta cggcgcggcc
     1741 ggctgggccg ccctggagcg ggtgcgggca cggattccgg ccgacgtccc gatgatcgcg
     1801 gacgccaagc gcggcgacat cgcctccacg tcggcccgcc aggtggtcgg ctacttcgac
     1861 tcgctcggcg tcgatgcggt cacgatcagc ccgtatctcg gccaggactc gatcgccggc
     1921 tacctcgagc ggccggaccg ctttgcctac gtcctgtgcc gcacgtcgaa cccgggcgcc
     1981 gccgacatcc aggggctccg ggtgcgcgcg cgctgggacg gcgaaggggt catgaccgga
     2041 cccgatgagc cgctttgggc gcgcgtggct cgcgacgctt cgtcgtgggg ccccggtggc
     2101 acggtcgggc tggtcgtcgg cgccaccgcg cccgaggagc tccgggccgc gcgttcgatc
     2161 gccccggggc tcgccttcct cgtgcccggc gtcggcgcgc agggtggcgg catcgagccg
     2221 gtgctcgccg ccgggcccgc aacggcggcg cccgcgggcg ggcggccggg tgggggactg
     2281 gtcgtcaacg tctcgcgagc catcgccgcc gcggccctcg agggaccgcc ggcggagctt
     2341 gaatcgcggc tcgccgaggc cgcccgaagc tggtccgcca agctccccgt gctaccctag
     2401 ccgccgttca gacggcccct gcggccgcac gcaaaaggac ctcgaatgcc gttcaacatc
     2461 ggccccggcg agctgatcat cgtcctcgtg atcgcgctcg tcgtgctcgg ccccggcaag
     2521 ctgcccgacg tggcggcgtc gctcggcaag agcctccgcg agttccggaa ggccgccacc
     2581 gacgtgagcg acgcgaccaa gatcgacgca ccgctgccct cgccgccggt tgccgccgtc
     2641 acgccgccgg cgccgatgcc cgccccggtc gcgccgcccg ccgtcgcggt gaacccggcc
     2701 gacccggctg acgcaggcgc cccggctgat gcaggcgccc cggcagcgac cggcgccgaa
     2761 cccgcgcgcg atccgaacgt tccctagctc cggcagcgcc ccccgcgccg cccgaccacg
     2821 cccatgacca cgcccatcac cgcgtccgac gccgccgagg agacccagca ggcacagatg
     2881 ccgctgtccg gccacctggc cgagctgcgc aaccggctga tctggtcgat cctcgcgatc
     2941 ggcctcggca gcatggtggg cttctacctc ggcgacgagc tcatcgcgat cctgcgcgcg
     3001 ccgctgccgc cgaatgtgcc cctgatcctg ctcgacgtca ccggcgcgtt ctcgatcagg
     3061 ctccaggtca gcctcgtggt cggcgtcgtg ctcgcgatgc cagtgatcct gttccacgtc
     3121 tggcgcttcg tggcgccggg gctcaccgcg tccgagcggc gcagcgtgct gccgtggatc
     3181 ccgatctcgc tcctgttctt cctgctgggc gtgtcgatcg cgtacatcat catgccgttc
     3241 gcggtgacgt tcctcctgag cttcgtgctc gaaggcgtcg acccgaccat ccagctgggc
     3301 agctacttca gcttcgtgac gagcctgttc ctggcgttcg gcatcctcat ggagttcccg
     3361 atcctgctga tcgggctgtc acgggtgaac atcgtcacgt ccgagctgct gagccgctcg
     3421 cgccggatgg tcatcctcgt catcgcgttg ttctcggcgg tcgcgacgcc gggcggcgat
     3481 ctcgtcagcc cgacgatcct cggcgtcacg ctgtacatcc tgttcgaggc gacggtcctg
     3541 ttcatccgcc gaaccgggcg ctgacgggct cgtagactgc cgctgatggc gaccgaaccg
     3601 acggccgagg gcaacgcccc gagcggtccg cacgtcgtgg tgctcacggg cctgtccggc
     3661 ggtggcaaga cggcggcggc gaagctgttc gaggaccttg cctacacggt cgtcgacaac
     3721 ctgcccggcg agctcctccc gaacctcgcg gagctcgtgg tccaggatcc cgcccgcttc
     3781 gcccgcgtcg cgatcgtcct cgacgtccgc gccggcgacg ccgagacggc atatcggcag
     3841 atgcgcggcg cgctcgaggg ccgcggcatc cggccccagg tgttcttcct cgaggccaac
     3901 gacgagacgc tgatccggcg cttctccgag acgcggcacc ggcacccgct ggcgatcgg
//
LOCUS       DATBSW010000147         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_576812, whole genome shotgun sequence.
ACCESSION   DATBSW010000147 DATBSW010000000
VERSION     DATBSW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576812"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VIF63_04070"
     CDS             <1..193
                     /locus_tag="VIF63_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238591.1"
                     /translation="LAAEPAKQVFSSDFRGRHRLPTASTLQAALKHLEEEGLVDRDAA
                     GAYQVTDPFFAGWLKRLVC"
     gene            193..1452
                     /locus_tag="VIF63_04075"
     CDS             193..1452
                     /locus_tag="VIF63_04075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238592.1"
                     /translation="MRPWLSDTLRSFRHRNFRLYYGGQLVSMTGYWMQQTAQGWLVLV
                     LTNSPGLLGLAGAVAGLPTLFLAVVAGVVADRMDRRRLLIITNGASALFALIMALLTT
                     FNVVEYWHILVLMFLTGLAFTVQLPASQAVVSSIVDRRSIGNAVALNSAQYNLLRIIA
                     PALAGLFIAADALALGFWVNAIALGLAAAIISRLTIPSERDVDRAQSAIWHDLQDGLR
                     YVMANRVLAIVVLLPGVPALFVLNYLTFIPVYARDILATGPAGLGLLTGAIGVGALTG
                     ALSLASLRPSGGSGRLVLGALVIVGFALMTFAVSRNLIVSMLALALIGAFQVNFYSTT
                     NTLIQVLVPARLRGRVLSLYLLTSIGLIPIANLVGGVVAEHSGVEIVLLGGGVLTVGI
                     ALATFVLEPRIGRLRAAHLSASEAAEA"
BASE COUNT          191 a          608 c          527 g          296 t
ORIGIN      
        1 gctcgcggcc gagcccgcca agcaggtgtt ctcctccgac ttccgcggac gccatcgcct
       61 gccgacggcg tcgacgctgc aggcggcgct caagcatctc gaggaggaag gtctcgtcga
      121 ccgcgacgcc gcgggcgcgt accaggtgac ggacccgttc ttcgccggct ggctcaagcg
      181 cctcgtctgc tgatgcggcc gtggctttcc gacacgctgc ggtccttccg gcaccgcaac
      241 ttccgcctgt actacggcgg acagctcgtg tcgatgaccg gctactggat gcagcagacg
      301 gcccagggct ggctcgtgct ggtgctcacc aactcgccgg gcctgctcgg cctcgccggc
      361 gcggttgccg gcctgccgac gctgttcctc gccgtggttg ccggcgtggt ggccgatcgg
      421 atggatcggc gtcggctgct catcatcacc aacggggcga gtgccctgtt cgcgctgatc
      481 atggccctgc tcacgacctt caacgtcgtg gagtactggc acatcctggt gctgatgttc
      541 ctgaccggcc tcgcgttcac ggtccagctg cccgccagcc aggcggtcgt ctcgagcatc
      601 gtcgaccggc gctcgatcgg gaacgcggtc gcgctgaact ccgcccagta caacctgctc
      661 cggatcatcg cgcccgcgct tgccggcctg ttcatcgcgg ccgacgccct cgcgctcggc
      721 ttctgggtca acgccatcgc gctggggctg gcggcggcga tcatctccag gctcacgatc
      781 ccctccgagc gtgacgtcga ccgggcccag tcggccatct ggcacgatct ccaggacggc
      841 ctgcgctacg tcatggccaa ccgggtcctc gcgatcgtcg tcctcctgcc gggcgtcccg
      901 gcgctgttcg tcctcaacta cctgacgttc atcccggtct acgcccgcga catcctcgcc
      961 acgggcccgg ccggcctcgg cctgctgacc ggcgcgatcg gcgtcggcgc gctcaccggc
     1021 gcactgtcac ttgcctcgct gcggccctcg ggcggcagcg gccgcctcgt cctcggggcg
     1081 ctggtcatcg tcggtttcgc gctcatgacc ttcgcggtct cgcgcaacct gatcgtgtcg
     1141 atgctggccc tcgcgctgat cggcgcgttc caggtcaact tctactcgac gaccaacacg
     1201 ctgatccagg tcctcgtccc ggcgcgcctg cggggtcggg tgctgagcct gtacctgctg
     1261 acgtcgatcg ggctcatccc gatcgcgaac ctggtcgggg gcgtggtcgc ggagcactcc
     1321 ggggtcgaga tcgtcctcct gggcggcggc gtgctcacgg tcggcatcgc actcgcgacg
     1381 ttcgtgctgg aaccgcgcat cggccgactt cgggccgccc acctgtccgc gagcgaggcc
     1441 gcggaggcgt agccgcccgg cttgcgtcgc cgcgccgagc cggtgctgaa accttttccg
     1501 cgttggtcgc ctcaggttgg tagcggacgg tcgcgatgac cgccacgagc accaaggagc
     1561 ccagcccaca tgttcacccg catcttcggc gtcgcgcgac ccatcctcgt cgcgctcgtc
     1621 gc
//
LOCUS       DATBSW010000148         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_47985, whole genome shotgun sequence.
ACCESSION   DATBSW010000148 DATBSW010000000
VERSION     DATBSW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47985"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VIF63_04080"
     CDS             <1..697
                     /locus_tag="VIF63_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8238593.1"
                     /translation="LNLSMFLAFDRTTVALALLGFYTYPALVAGASGFLGREPLDATR
                     VVALVLALAGMVAVVLGGLGTTEQVRVDAIGIGLGLFAAVLQTTFVLTSRGFAAVRAE
                     TAMGVILAGSALVAAVASIATGGAAALAQPLQQPSVVAMLVAVGLFGAALPSYLFLTA
                     IRWIGGVRTGILMLFEPVVGVALAAVFLAEGLQPLQAVGGATILAAALIVQRGTRIQS
                     ETGAAIAPAPGGP"
     gene            694..1488
                     /locus_tag="VIF63_04085"
     CDS             694..1488
                     /locus_tag="VIF63_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8238594.1"
                     /translation="MSEQPIERPNALPNEQQIVHWDVTGPPDAAPIVFVHGAMMGRSV
                     WRPQIDALADRYRCISVDLPGHGTQRDNAFSIDEAAAGVIRAIDEAAGGRAVLVGLSL
                     GGYIAMTVAGHHPERVRGLVIAGSTREPAGVSRLAFQLYGWGLRLAPQPAVRAIALAW
                     FRRRYGPVLGAAITAGGHFAQGGGRAVLHLARGRFKDRLIAYGGPILVINGSMDLVFR
                     IGAGRFLAGVPGVRSHMISRAGHLSNVDKPAAFTGLVEEFIDSLEP"
     gene            1559..>1935
                     /locus_tag="VIF63_04090"
     CDS             1559..>1935
                     /locus_tag="VIF63_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8238595.1"
                     /translation="MRVRKAVFPAAGWGTRFLPATKAQPKEMLPLVDKPIIQYGVEEA
                     VAAGIEQIIIVTSTHKGAIEDHFDLNYELEKVLEEKGEIEKLRQVRAITDLAQLAYVR
                     QKEQLGLGHAILMAKDLIGHEPFA"
BASE COUNT          257 a          708 c          665 g          305 t
ORIGIN      
        1 cctcaacctc tcgatgttcc tcgcgttcga ccgaacgacg gtcgcgttgg ccctgctcgg
       61 cttttacacc tacccggcgc tggtcgccgg cgcgagcggg ttcctcggac gggagccgct
      121 cgatgcgaca cgggtcgttg cgctcgtcct tgccctcgca ggcatggttg ccgtcgtcct
      181 cggcggactc ggcacaaccg aacaggtccg cgtggacgcg atcgggatcg ggctgggcct
      241 cttcgcggcc gtgctccaga cgaccttcgt gctgacgagc cgaggattcg cggcggtccg
      301 ggccgagacg gccatgggcg tgatcctcgc cggctcggcg ctcgtcgctg ccgtggccag
      361 catcgcgacc ggcggcgccg cggccctcgc acagcccttg cagcagccgt cggtcgtggc
      421 gatgctcgtg gccgtcgggc tgttcggggc ggccctgccg tcgtacctct tcctcaccgc
      481 gatccgttgg atcggcggtg tgcgaaccgg gatcctgatg ctgttcgagc ccgtggtcgg
      541 ggtggccctc gcggcagtgt tcctcgccga gggcctccag ccgctccagg ccgtcggggg
      601 tgccacgatc ctcgccgccg cgctcatcgt ccagcgcggc acgcgcatcc agtcggagac
      661 cggtgcggcg atcgcgcccg ccccgggcgg cccatgagcg agcagcccat cgagcggccc
      721 aacgcgctac ccaacgagca gcagatcgtc cactgggacg tcaccggtcc gcccgacgcc
      781 gccccgatcg tgttcgtcca cggggcgatg atgggtcgct cggtctggcg cccccagatc
      841 gatgcgctgg ccgaccgcta ccgctgcatc agcgtcgacc tgcccggcca cggcacgcaa
      901 cgcgacaacg cgttctcgat cgacgaggcg gccgccggcg tgatccgggc gatcgacgag
      961 gcagccggcg gccgggcggt tctggtcggc ctgtcgctcg gtggctacat cgcgatgacc
     1021 gtggccggcc accaccccga gcgagttcgc ggcctcgtga tcgccggctc cacgcgcgag
     1081 ccggcaggcg tcagccggct ggcgttccag ctctatggct ggggcctgcg cctcgcgccg
     1141 cagccggcgg ttcgggccat cgcgctggcc tggttccggc gccgctacgg gccggtcctc
     1201 ggggccgcga tcaccgccgg cggccatttc gcgcagggcg gcggacgcgc ggtcctgcac
     1261 ctcgcgcgcg gccggttcaa ggaccggctt atcgcctacg gcgggccgat cctcgtcatc
     1321 aacggctcga tggacctcgt cttccggatc ggcgcgggcc ggttcctcgc gggcgtgccc
     1381 ggcgtccgca gccacatgat ttcgcgagcc gggcaccttt cgaacgtcga caaaccggcg
     1441 gccttcaccg gcctcgtgga ggagttcatc gactcgctgg aaccctagtt cagagggtgc
     1501 cggtgctatc ctcccggcgc agggccacac gttccgggcc cggtccacgt gaggttcgat
     1561 gcgcgtccga aaggctgtct tccccgcggc cggttgggga acgaggttcc tccccgcgac
     1621 gaaggcccag cccaaggaga tgctcccgct cgtcgacaag ccgatcatcc agtacggcgt
     1681 cgaagaggcc gtcgcggcgg ggatcgagca gatcatcatc gtcacctcga cccacaaggg
     1741 cgccatcgag gaccacttcg acctcaacta cgagctcgag aaggtcctcg aggagaaggg
     1801 cgagatcgag aagctccgcc aggtgcgggc gatcaccgac ctcgcccagc tcgcctacgt
     1861 gcgccagaag gagcagctcg gcctgggcca cgcgatcctg atggccaagg acctcatcgg
     1921 ccatgagccg ttcgc
//
LOCUS       DATBSW010000149        11217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_612561, whole genome shotgun sequence.
ACCESSION   DATBSW010000149 DATBSW010000000
VERSION     DATBSW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612561"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VIF63_04095"
     CDS             complement(<1..1153)
                     /locus_tag="VIF63_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8238596.1"
                     /translation="MPDDVPPGSLVEPGPADTLLALDRATRAIAGELDLDRVLQLIVD
                     SVRELVGSRYAALGTFDAKGRIERFITSGMDPAVRQEIGPLPQGHGLLGTIIREGRTL
                     RIPDIAMHPDSFGFPAHHPPMHSLLGVPIGIAGAIVGNFYLTEKEHAPEFSAADEELV
                     ERFAGHAAIAIQNARLHNEVQKLAIVDERLRISRDLHDGIIQGIYAVSLSLEDVAELI
                     DVDRTGALERVDRAIDRLHTTIGEIRTFIVGLGPEGGNGLPGALESMARELLAGTDIK
                     LTLDLAGAAELGGRLTLEAAHELSQIAREALSNVARHSGATHALLSVGVDDDVATLRI
                     EDDGVGFDPRLPRGRSHFGLANLRSRAAAVAGSLLIESEPGKGARIIVRM"
     gene            1264..2553
                     /locus_tag="VIF63_04100"
     CDS             1264..2553
                     /locus_tag="VIF63_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8238597.1"
                     /translation="MPNQPFPARLDAPVIDMDSFAPAPDLSRRRFLRGAVIAGGGAIV
                     ATIAACTPSSPLPGWTYPAGVPTGATSTTQPSAAPAASMDHVASPGASPAASPGGHDE
                     NALAVVKRFLDGESAALEGAGNQPYGDPTVDAGVKVFDLTVDKITHRIEAQRDPIDAL
                     GFNGTWPGPRIDVIEGDKVRATFKNNLDESTGIHFHGQRLPNNMDGVPHITQDPIEPG
                     ASFSYEFTARTVGSHMYHSHHNATDQVGRGLLGAFIVHPKDPAQRVERKYGVSQDIVW
                     ISNDSLGGFTINGRGFPATAPIVATLGDKILIRFMNEGVMMHPWHLHGTPMRVVARDG
                     YDLGPAAFTCDTLGVNPGERWDVVIDCDAPGAWAFHCHILPHAEGRDGMFGMVTALVV
                     QEPASAAAVLLGTTTAASPVRSAAAAAGSTAWMCRIG"
     gene            2550..3020
                     /locus_tag="VIF63_04105"
     CDS             2550..3020
                     /locus_tag="VIF63_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8238598.1"
                     /translation="MSALPATASDPASGADRSIRRILLATDLSTASEGASEQALQLSR
                     DLRADLLVVSVIDPASVLPLGPSSARMDQRRAAREAVAQAIVVNGVRAGVKVNFLVWE
                     GEPGPAIVEAAIAEDADLVVVGTHGRSRVGRMVLGSVSDHVVRHAPCPILIVRG"
     gene            3232..3930
                     /locus_tag="VIF63_04110"
     CDS             3232..3930
                     /locus_tag="VIF63_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238599.1"
                     /translation="MYGRMRIVGIVLAVIGIGFLAGGGYAVVKTQQGFTSLNALSAAQ
                     NVKLSYNDQGQLVDRGETAGAEAIMSLLRDDWKYPVDSAELNPNDPVVNTASEYMYQM
                     ATITYHTLHGTQSVVLAKDVTAADGTVYPAGTYEFAVDGRYWSAFNRANPIEAIAREQ
                     AWTGTAHGLIANLGVGTATATALQLGLGIAGLFAGIGLFALIAGFGLIWVARAATAEE
                     RAPKVVLQRQAMPV"
     gene            4036..4803
                     /locus_tag="VIF63_04115"
     CDS             4036..4803
                     /locus_tag="VIF63_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8238600.1"
                     /translation="MAKQLVAPTRRERRAAERADRPRGPRHAPAVRRRFSLEKLTAIA
                     LVGGIGVVALAIAFGARPSTPSAHTEVAVAFAPAGVATDGFVIGSPDAAVTIELYEDF
                     QCPACELWGQTVFPTLAANELADGTVRVVFHDVAFLGQESTDAGMAAYAAGQQGRFWD
                     MWATLYANQGHENSGAFSRERLVAMADHLGLDVARFEVDMDSSEATAALAASKAAAGR
                     VGVTSTPTLIVDGQVLLGVRPYPELAAAIAAAASAAP"
     gene            4800..5270
                     /locus_tag="VIF63_04120"
     CDS             4800..5270
                     /locus_tag="VIF63_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY8238601.1"
                     /translation="MTAVGPAIARLRAIHPGVVLATLDVIGLVIAAYLSVVELQGELP
                     YCGPLKGCEQVALSEYARIGGIPVAVFGVILSMALLVLAIAWTRSGRVELLAAHYGLS
                     LVGVIFEIYFTYLELFVIGAVCIWCASYGVSLVARFGVALYVWRRRADPRAAMA"
     gene            complement(5267..6058)
                     /locus_tag="VIF63_04125"
     CDS             complement(5267..6058)
                     /locus_tag="VIF63_04125"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8238602.1"
                     /translation="MTAQAGTPPFSKNEMMIVAAARELAGQHVCFVGVGLPNIAVNLA
                     QRTVAPDLELVYEAGVFGARPARLPLSIGDPTIVTGATAVTSMFELFSFYLQGGLIDV
                     GFLGAAQIDRFGNLNSTVIGDYDAPTTRLPGSGGACEIAINAREVFVIMRQSTRSFVE
                     TIDFRTSAGNLGGAEQSERIRREQGWLGRGPSVVVTDLAVWHFGDDGEMRVDSLHPGV
                     SIDDVRSTVAWEPKVAASLATTPAPSADELRLIREELDPGGAYTR"
     gene            complement(6081..7037)
                     /locus_tag="VIF63_04130"
     CDS             complement(6081..7037)
                     /locus_tag="VIF63_04130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8238603.1"
                     /translation="MSTSKVASMHDAVAAVVRDGDTVAIEGFTHLISFAAGHEIIRQR
                     KRDLTLARLTPDLIYDQMIAAGVARKLIFSWLGNPGVGGLGAIRRRTEPSTSARAGDP
                     DAEGRLPRIEIEEYSHFGMVGRYTAGAANLPFYPLRSYFETDLPKANPLIREIDSPYG
                     DGVVYAVPPLKPDVTIVHAQRADAAGNTQVWGLLGCQKEAAFAADRVIVVVEELVDES
                     VVRADPNRTIIPGLIVDAVVVEPYGAHPSYVQGAYDRDNRFYLDWDAITKDEAALQAW
                     LKEWVYGVDSRAAYIEKLGGDRIASLKPGSAPSGSVDYGEYR"
     gene            complement(7363..8745)
                     /locus_tag="VIF63_04135"
     CDS             complement(7363..8745)
                     /locus_tag="VIF63_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8238604.1"
                     /translation="MAQSVSALDRASLPISERPLPDLITEIPGPKARAAVKFDEEWTS
                     PSLPRAYPIVPVRGRGMAIEDIDGNLFLDFAAGIAVNSTGHAHPRVVGAIKEQASELL
                     HYSASDFYTPIYAETAAAIARIAPMQGKLRTYLGNSGAEVVEAAMKLARHYTGRPYIV
                     GFLGGFHGRTYGAVSLTASKAKYHARFDPLLPGIYHAPYGKVEDLKWFDDVLFNRLIP
                     ADEVAAMVIEPIQGEGGYVVPEDGFLQGLRELCTKHGILLVADEIQSGAGRTGKMWAI
                     EHWNVEPDILLVAKGIASGMPLGAMVAPAKIMESWGIGAHGSTYGGNPVACAAALATI
                     ELLQGGLIDNAAARGEEAFAGLREVWSRNPKTVIDVRGKGLMIGVEFASPELAEEVQW
                     ACFQRGLLVLECGAQTVRLCPPLVASSSDIATAVRIFGEAVADVAAHPAEAAHAAAAA
                     GAMHDGEVDG"
     gene            8906..9466
                     /locus_tag="VIF63_04140"
     CDS             8906..9466
                     /locus_tag="VIF63_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238605.1"
                     /translation="MPKRTRATSWSGRTAGLFALALVAGCAAPAAPGPASTAIATTGV
                     GGTAAATAAATSGPPAATAPIQTPLGAQTAMVDLTFTGDISLVAKGSAGDCQLGEDAG
                     GSAIGFGFTATDSDYKGLGDSFNITEDIASHKVSVKWVVAGTFYAGVFASGATVAPDH
                     HSVTFDADIPAGVGRTEHLKGSITCP"
     gene            complement(9488..10369)
                     /locus_tag="VIF63_04145"
     CDS             complement(9488..10369)
                     /locus_tag="VIF63_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238606.1"
                     /translation="MPPRRAAPIVVALLASLVAVACTPTAPTPSPGSTDSATGAPSTV
                     GPSASEDPGNARLKPSNAVAVIVDRLPVRFEPSPAGESQGDLVRGDVAVLQAFEPVEL
                     DGVVWYYATQVGTDGPGKLPELPFVNPVEETLAGWIAASDATGETIAKLAPRCPETWD
                     YTNLSAMLDGERLACFGAESITLDGRFGCVGCGAEPGTFEPAWLAGAGAGSLSAVGAS
                     GPPWIPLHFPVAVEAPANEAYVRVTGHFDDPEAITCQMAVDPKPRSFAIAVELCRQRF
                     VVDSWEPLSSEFIPPPD"
     gene            complement(10372..>11217)
                     /locus_tag="VIF63_04150"
     CDS             complement(10372..>11217)
                     /locus_tag="VIF63_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8238607.1"
                     /translation="GDTVGEELQSNPGIDGIVFTGSYEVGMRLYRSFSSTWPRPTIVE
                     MGGKNPAIVARSADLDEAAEGILRSAFGFGGQKCSANSRVYVEKPVHDELVRLLVEKT
                     EKLTVGNPIQRENWLGPVVDQKAVDRHQQAVAEARRDGKVYTGGEHLSDGDLARGYYV
                     EPTVVGNLPASHRVFEDELFAPFTAVQSVDSLDEALRLANDTIYGLTAGVYSEDPSEV
                     ARFLDEAQAGVLYVNRRAGATTGAWPGVQAFGGWKGSGSTGKSGLSMYYVAQFLREQS
                     HTVVD"
BASE COUNT         1751 a         3935 c         3815 g         1716 t
ORIGIN      
        1 gcattcgaac gatgatacgc gcgcccttgc ccggttcgct ctcgatcagc aatgagcccg
       61 cgacggctgc ggcacggctg cgcaggttcg cgagaccaaa gtgactcctg ccccgcggca
      121 gtcgtgggtc gaacccgacg ccatcgtcct cgatgcgcaa cgttgcgaca tcgtcatcca
      181 cgcccaccga gagcagggcg tgcgtcgcgc cactgtgccg ggcaacgttg ctgagcgctt
      241 cacgggcaat ctggctgagc tcgtgcgccg cttcgagcgt gagccgtccg cccagctccg
      301 cggcgccggc gagatcgagc gtgagcttga tgtcggtgcc ggcgagcagc tccctggcca
      361 tcgattcgag ggccccggga agaccgttgc cgccctcggg acccaggccg acgatgaacg
      421 tccggatctc gccgatcgtg gtatggagcc ggtcgatcgc ccgatcgacc cgttcgagcg
      481 cgcccgtccg gtccacgtcg atcagctcgg caacgtcttc cagcgacagg ctcaccgcgt
      541 agatgccctg gatgatcccg tcgtggaggt cgcgactgat gcgcaggcgc tcgtcgacga
      601 tggcgagctt ctggacctcg ttgtggagcc gggcgttctg gatcgcgatc gcggcgtgcc
      661 ccgcgaagcg ctcgacgagc tcctcgtccg ccgcgctgaa ctcgggagcg tgctccttct
      721 cggtgaggta gaagttgccg acgatcgcgc cggcgatgcc gatcgggacg ccgaggagcg
      781 agtgcatcgg gggatggtga gcggggaacc cgaatgaatc cgggtgcatg gcaatgtccg
      841 ggatccgaag cgtccgaccc tcgcggatga tcgtgcccag gaggccgtgg ccctggggca
      901 gcggcccgat ctcctggcga acggcggggt ccatgccgct cgtgatgaac cgctcgatcc
      961 ggcccttggc atcgaacgtc ccgagggcgg cgtaacgcga cccgaccagc tctcggacgc
     1021 tgtcgacgat cagctggagg acccgatcga ggtcgagctc gcccgcgatc gcgcgcgtgg
     1081 cgcgatccag ggcaaggagg gtgtcggcag gcccgggctc gaccaacgat ccgggcggga
     1141 cgtcatctgg cacggcgagg agcctaccag cgccgggcat ggcgaccgac cgggcgtgat
     1201 ggcccatggt tttcgggcca ctcgtcactt cgtgccggct gcgaccgacc gcaccatccc
     1261 cccatgccga accagccttt cccggcccgc ctcgacgcgc cggtcatcga catggactcc
     1321 tttgcgcctg cgcctgacct cagtcggcgc aggttccttc gcggcgcggt gatcgccggg
     1381 ggtggcgcca tcgtcgccac catcgcggcc tgcacgccca gctctccgct ccccggctgg
     1441 acctaccccg cgggcgtgcc gaccggagcc acatcgacga cccaaccgag cgcagcgccg
     1501 gccgcgtcga tggaccacgt cgccagccct ggtgcgagcc cggctgcgag cccgggcggc
     1561 cacgacgaga acgcgttggc ggtcgtgaag cggttcctcg acggcgagtc cgccgcgctc
     1621 gagggcgctg gcaaccagcc atacggcgac ccgaccgtcg acgccggggt caaggtcttc
     1681 gacctgacgg tcgacaagat cacccaccgg atcgaggcgc agcgcgaccc gatcgacgcg
     1741 ctcggcttca acggcacgtg gccgggtccg cggatcgacg tcatcgaggg cgacaaggtc
     1801 cgggccacgt tcaagaacaa cctcgacgag tcgaccggaa tccatttcca cggccaacgg
     1861 ctcccgaaca acatggacgg cgtcccgcac atcacccagg atccgatcga gccgggggcg
     1921 tcgttcagct acgagttcac ggcgaggacc gtcgggtcgc acatgtacca ctcgcaccac
     1981 aacgccacgg accaggtcgg ccgcggattg ctgggcgcgt tcatcgtcca cccgaaggac
     2041 ccggcccagc gtgtcgagcg caagtacggc gtgagccagg acatcgtctg gatcagcaac
     2101 gactcgctcg gcgggttcac gatcaacggc cggggcttcc ccgcgaccgc gccgatcgtc
     2161 gcaaccctcg gcgacaagat cctgatccgg ttcatgaacg agggcgtgat gatgcatccc
     2221 tggcatctgc acgggacgcc gatgcgggtc gtcgcgcgcg acggctacga cctgggcccg
     2281 gccgccttca cctgcgacac gctcggcgtg aatcccggcg agcgttggga cgtcgtgatc
     2341 gactgcgacg cgcccggcgc ttgggcgttc cattgccaca tcctgcccca cgccgagggc
     2401 cgcgacggca tgttcggcat ggtcaccgcg ctcgtcgtgc aggagccggc cagcgcggcc
     2461 gccgtcctgc tcggcaccac gaccgccgcc agtcccgttc gatctgcagc cgccgcggcc
     2521 gggtcgacgg cctggatgtg tcggatcgga tgagcgcgtt gccggccact gcgagcgatc
     2581 ccgcctcggg tgccgatcgg agcattcggc ggatcctgct cgcgaccgac ctctcgaccg
     2641 cctccgaggg cgcgtccgag caggcgctcc agctctcgcg cgatctgcgc gcggacttgc
     2701 tcgtcgtcag tgtgatcgac cctgcgtccg tcctccccct ggggccgagc tctgctcgca
     2761 tggaccagcg ccgcgccgcg cgagaggccg tcgcgcaggc catcgtcgtc aacggcgtga
     2821 gggcaggcgt caaggtcaac ttcctcgtct gggagggcga gcccgggccc gcgatcgtcg
     2881 aggcggcgat cgccgaggac gccgacctcg tggtggtcgg cacgcacggc cgcagccgcg
     2941 tgggacgcat ggtgctcggc agcgtgtcgg accacgtcgt tcgccacgcg ccctgcccga
     3001 tcctgatcgt tcgcggctga gcgagcgagc caccaagggc cgccaccgat ccccgcggca
     3061 caaggaacgc cctccccgat agtcccgaga tacccgggac agatggcacg gatgccgtgg
     3121 gccgacccgc ccctcattcg gtccgttcgg cagcgtccca tcccggccct cagcggcgaa
     3181 agtgcaccta tcgacaaagg tcgagcaaca gaggcgaagg gagaacttca gatgtacgga
     3241 cggatgcgga tcgtgggcat cgtgcttgcg gtcatcggaa ttggtttcct tgccggcggc
     3301 gggtatgccg ttgtcaagac ccagcagggc ttcacctccc tgaacgcgct cagcgcggcc
     3361 cagaacgtca agctgagcta caacgatcag ggccagctcg tcgatcgtgg cgagaccgcc
     3421 ggagccgagg cgatcatgtc gctgctccgc gacgactgga agtacccggt cgacagcgcc
     3481 gagctcaacc ccaatgaccc ggtcgtgaac acggcttctg agtacatgta ccagatggcc
     3541 acgatcacct accacacgct ccacggcacg cagagcgtcg tgctcgccaa ggacgtgacg
     3601 gctgcggacg gcacggtcta cccggccggc acgtacgagt tcgccgtcga cgggcgctac
     3661 tggagcgcat tcaaccgcgc caacccgatc gaggccatcg ctcgcgagca ggcctggacc
     3721 ggcacggccc acgggctgat cgccaacctg ggcgtcggaa ccgcaacggc cacggccctc
     3781 cagctcggcc tcgggatcgc agggctcttc gcaggcatcg ggctcttcgc cctcatcgca
     3841 ggcttcggcc tgatctgggt cgctcgagcc gccaccgccg aggagcgcgc accaaaggtc
     3901 gtcctccagc gacaggcgat gcccgtctga gcgcaggctg ggaggcggta cctcccgacc
     3961 acacccccag ctcacccgga cccggcttcc ctcgaagccg ggtccgttcc tgatcagcga
     4021 atggagcgcc atcccatggc aaagcagttg gttgcaccga cgaggcgaga gcgacgggca
     4081 gcggagcgcg cagatcgccc gcgcggacct cggcacgcac cggccgttcg ccgcaggttc
     4141 agcctcgaga agctgacggc catcgcgctc gtggggggta tcggggtcgt cgccctcgcg
     4201 atcgcgttcg gagctcgacc atctacccca tcggcgcaca ccgaggtcgc ggtggccttc
     4261 gcccctgcgg gcgtggcgac cgatggcttc gtgatcggct caccggacgc cgcggtcacg
     4321 atcgagctgt acgaggactt ccaatgccct gcctgcgagc tctgggggca aaccgtcttc
     4381 ccgaccctgg cggcgaacga gctcgctgat ggaaccgtgc gcgtggtctt tcacgacgtc
     4441 gcgttcctcg ggcaggagtc gacagacgcc ggcatggcgg cgtacgcagc agggcaacaa
     4501 ggccggttct gggacatgtg ggcgacgctc tacgccaacc agggccatga gaactctggc
     4561 gcgttcagcc gagaacgact ggtcgccatg gccgatcacc tcgggctcga tgtcgcccgc
     4621 ttcgaggtcg acatggactc gtccgaggcg accgcggcgc tcgcagcctc gaaggccgcg
     4681 gcgggtcgcg tcggcgtgac atcgaccccg acgctcatcg tggacggcca ggtcctcctt
     4741 ggtgtcaggc cgtatccgga gcttgcagcc gcgatcgcag ctgcggccag cgcggcgcca
     4801 tgaccgccgt cggaccggcg atcgcgcgat tgcgggcgat ccatccgggc gtcgtcctcg
     4861 ccacgctcga cgtcatcggg ctcgtgatcg ccgcgtacct gagcgtcgtc gagctccagg
     4921 gcgagctgcc gtactgcggt cccctcaagg gctgtgagca ggtcgccctt tccgagtacg
     4981 cgcgaatcgg cggcatcccg gtcgcggtgt tcggcgtgat cctgtcgatg gccttgctcg
     5041 tcctggcaat tgcctggacg cggtccggcc gcgtcgagct gctcgccgcc cactacggcc
     5101 tgtcgctcgt tggcgtgatc ttcgagatct acttcaccta cctcgagctg ttcgtgatcg
     5161 gggcggtctg catctggtgc gcgtcctacg gcgtgagcct cgtggcgcgg ttcggcgtgg
     5221 cgctgtacgt gtggcgccga cgcgccgatc cgcgggcggc gatggcctag cgggtgtagg
     5281 cgccgccggg atcgagctcc tcgcggatca ggcgcagctc gtcggcggac ggggccggcg
     5341 tggtcgcgag cgacgccgca accttcggct cccaggcgac ggtcgagcgg acgtcgtcga
     5401 tggagacgcc cgggtgcagc gagtcgaccc gcatctcccc gtcgtcgccg aagtgccaca
     5461 cggcgagatc ggtgacgacc accgacgggc cgcgaccaag ccagccctgc tcccgtcgga
     5521 tccgctcgga ctgctcggcc ccgcccaggt tgccggcgga cgtccggaag tcgatcgtct
     5581 cgacgaagct ccgcgtcgac tggcgcatga tcacgaacac ctcgcgggcg ttgatcgcga
     5641 tctcgcacgc cccgcctgat cccgggaggc gcgtcgtggg tgcgtcgtaa tcgccgatga
     5701 cggtcgagtt caggttgccg aatcgatcga tctgggcggc gcccaggaag ccgacatcga
     5761 tcagcccgcc ctgcaggtag aagctgaaca gctcgaacat gctggtgacc gcggtggcgc
     5821 cggtgacgat cgtggggtca ccgatcgaca gcgggaggcg ggccggccgg gccccgaaga
     5881 cgcccgcctc gtacacgagc tccaggtccg gcgcgaccgt ccgctgggcc aggttgaccg
     5941 cgatgttcgg cagcccgacg ccgacgaagc acacgtgctg gccggccagc tcgcgcgcgg
     6001 cggccacgat catcatctcg ttcttggaaa acggcggcgt gcccgcttgg gccgtcatcg
     6061 acgcgcctcg aacagcgcca tcagcggtac tcgccgtagt cgaccgagcc cgacggcgcg
     6121 gagccgggct tgagcgacgc aattcgatcc ccgcccagct tctcgatgta ggccgctcgg
     6181 ctgtcgacgc cgtagaccca ctccttgagc catgcctgga gcgcggcctc gtccttcgtg
     6241 atcgcgtccc agtcgaggta gaagcggttg tcgcggtcgt atgcgccctg gacatacgac
     6301 ggatgcgcgc cgtacggctc gaccacgacg gcatcgacga tgagccccgg aatgatcgtc
     6361 cggttcgggt ccgcccgaac gacggactcg tcgacgagct cctcgacgac cacgatcacg
     6421 cgatcggcgg cgaaggccgc ctccttctgg cagccgagga ggccccagac ctgcgtgttg
     6481 ccggcggcgt cggcgcgctg ggcatggacg atcgtgacgt ccggcttgag cggcggcacg
     6541 gcgtagacca ccccgtcgcc gtatggagaa tcgatctcgc ggatcagcgg gttcgccttg
     6601 ggcaggtcgg tctcgaagta gctgcgaagc gggtagaacg gcaggttggc ggcgcccgcc
     6661 gtgtaccgcc cgaccatgcc gaagtggctg tattcctcga tctcgatgcg cggcagcctg
     6721 ccctcggcgt ctgggtcgcc ggcgcgggcg ctcgtcgagg gctcggtccg ccgccggatc
     6781 gcaccgaggc caccaacccc agggttgccg agccacgaga agatcagctt gcgggcgacg
     6841 cccgcggcaa tcatctggtc gtagatgagg tcgggtgtga gacgggcgag cgtcaggtcg
     6901 cgctttcgct gccggatgat ctcgtggccc gccgcgaacg agatgaggtg cgtgaacccc
     6961 tcgatcgcga ccgtgtcccc gtcccgcacg accgcggcaa cggcgtcgtg catcgacgcg
     7021 accttgctcg tgctcaccgg gcgatgatgc cagccgtatg cgtgccgcag cgatccgtcg
     7081 tgcggtcgcc acgatggccc aatcggattg gcgtgatcac cgggcgatca ttttcccggc
     7141 ttgcggcacc gattcatcgc cgacagcccg attcatcgcg tcatccgggc tgacatgcaa
     7201 cgaagtcggc gtcgaatccg tgcgattcgt gctcacctct acgtcaccct cgagacgacc
     7261 cgtgcgtccg ggacagggcc agcaacgaac tgcgctcgcg acccctggaa atgatcatca
     7321 cgtgaacttc tgatctgaga tggctcggca ctaccagcgg aactacccgt cgacctcgcc
     7381 gtcgtgcatc gcgccggccg cagccgccgc atgcgcagct tccgcggggt gtgcggcgac
     7441 atcggcgacg gcctcgccga agatccggac ggccgtcgcg atatcggacg acgacgcgac
     7501 caggggtggg cacagccgaa ccgtctgcgc gccgcactcg aggacgagca ggccgcgctg
     7561 gaagcacgcc cactggacct cctcggcgag ctcgggcgag gcgaactcga cgccgatcat
     7621 caggcccttg ccgcgcacgt cgatgacagt cttcggattc cgcgaccaga cctcgcgcag
     7681 gcctgcgaac gcctcttcgc cgcgcgccgc cgcgttgtcg atgagcccgc cctggagcag
     7741 ctcgatggtc gcgagggcag ccgcgcaggc gaccgggttg cccccatatg tcgagccgtg
     7801 agcgccgatg ccccacgatt ccatgatctt ggccggcgcg accatggccc ccaggggcat
     7861 gcccgaggcg atgcccttcg cgacgagcag gatgtccggc tcgacgttcc agtgctcgat
     7921 ggcccacatc ttgcccgtgc ggccagcgcc ggactggatc tcgtccgcca cgagcaggat
     7981 gccgtgcttc gtgcacagct cgcgcaggcc ctggaggaag ccgtcctcgg ggacgacgta
     8041 gccgccctcg ccctggatcg gctcgatcac catcgccgcg acctcgtcgg ccgggatcag
     8101 gcggttgaac aggacgtcat cgaaccattt gagatcctcg accttgccgt acggcgcgtg
     8161 atagatgccg ggcaggagcg gatcgaagcg ggcgtggtac ttggccttcg acgcggtgag
     8221 cgacacggct ccgtacgtcc gaccgtggaa cccacccagg aagccgacga tgtacggccg
     8281 gccggtgtag tggcgcgcca gcttcatcgc cgcctcgacc acctcggcgc ccgagttgcc
     8341 gaggtacgtc cggagcttgc cctgcatcgg cgcgatgcgc gcgatggcgg ccgcggtctc
     8401 cgcgtagatc ggcgtgtaga agtcggacgc ggagtaatgc agcagctcgc tggcctgctc
     8461 cttgatcgcg ccgacgacgc gcgggtgggc gtggcccgtc gagttcacgg cgatgccggc
     8521 cgcgaagtcg aggaacaggt tgccatcgat gtcctcgatc gccatgccgc ggccacggac
     8581 gggcacgatc ggatacgccc gtggcaggct cggtgatgtc cattcctcgt cgaacttcac
     8641 ggccgcgcgg gccttcgggc cggggatctc ggtgatcagg tccggcagcg gacgctcgga
     8701 gatcgggagc gacgcgcgat cgagcgcaga aacggactgg gccaccgaac acctcgaggc
     8761 aatgcatagg aactatgcag agtggataga gtgggccgag tctagcacgc agattcctcg
     8821 aatccggact gacgcggcga cgcgtcgtca cctatcgtcg cgagcgccaa gcccatcccg
     8881 gaggcgctgc cagatgccct caccgttgcc caaacggacg cgcgccacgt cctggtccgg
     8941 ccggaccgcg ggactgttcg ccctcgcgct cgtcgcgggc tgcgcagccc ctgccgcacc
     9001 aggcccggcc tcgaccgcga tcgcgaccac tggtgttggt ggcacagcgg cggcgaccgc
     9061 cgctgccacg agcgggccac ccgccgccac cgcgccaatc cagacgcccc tcggcgctca
     9121 gacggccatg gtcgacctca cgttcacggg cgacatctcg ctcgttgcga agggctcggc
     9181 cggcgactgt cagctcggag aagacgcagg cgggtccgcg atcggctttg gattcacggc
     9241 aaccgatagt gactacaagg gtctgggcga cagcttcaac atcaccgagg acatcgcatc
     9301 ccacaaggtc tcggtgaagt gggtcgtcgc cggcacgttt tacgccggcg tgttcgcaag
     9361 cggcgccacc gtggcgccgg accaccattc ggtcacgttc gacgcggaca tccccgcagg
     9421 tgtcggacga accgagcacc tgaagggctc gatcacctgt ccgtgacgcg tgctggctgc
     9481 gccggcctca gtccggcggc gggatgaact cggagctcag cggctcccag gaatcgacga
     9541 cgaaccgctg acgacagagc tcgacggcga tcgcgaatga gcgcggtttg gggtcaacgg
     9601 ccatctggca ggtgatggcc tcgggatcgt cgaaatggcc cgtgacgcgc acgtatgcct
     9661 catttgccgg cgcctcgacc gccacaggga aatggagcgg aatccagggc ggtcccgatg
     9721 cgccgacggc gctgagcgag ccagcgccgg cgccggccag ccaagcaggt tcgaacgtcc
     9781 ccggctcggc gccacagccc acgcacccga acctgccgtc gagggtgatc gactcggcgc
     9841 cgaagcatgc caggcgctct ccatcgagca tggcggacag gttcgtgtag tcccaggtct
     9901 ccgggcagcg gggtgcgagc ttggcgatcg tctcgcccgt cgcatcgctc gccgcgatcc
     9961 aacccgccag ggtctcctcg accgggttga cgaagggcag ttcgggcagc ttcccgggac
    10021 catccgttcc aacctgtgtc gcgtagtacc agacgacgcc atccagctcg accggctcga
    10081 atgcctgcag gaccgcgacg tcaccacgca cgaggtcgcc ctgactctcc cccgccggcg
    10141 acggctcgaa acggaccggc aatcggtcga cgatcaccgc gaccgcgttg gacggcttca
    10201 gccgggcgtt ccctgggtcc tcgcttgcgg atggaccgac ggtcgatgga gcgccggtcg
    10261 cgctgtcggt gctgccaggg cttggagtcg gcgctgtggg cgtgcaggcc acagccacca
    10321 ggctcgcgag gagcgcgacg acgatcggcg cggcgcgccg aggaggcatc ctcagtcgac
    10381 gaccgtgtgg ctctgctcgc gcaggaactg ggcgacgtag tacatcgaga gaccggattt
    10441 tccggtcgac ccggagcctt tccagccgcc gaacgcctgc acgccaggcc acgcgccggt
    10501 cgtcgcgccg gcgcggcggt tcacgtacag gacgcccgcc tgggcctcgt cgaggaagcg
    10561 cgcgacctcg ctcggatcct cgctgtacac gcccgcggtc aggccgtaga tcgtgtcgtt
    10621 ggcgaggcgc agggcctcgt cgagcgaatc gaccgactgg accgccgtga acggcgcgaa
    10681 cagctcatcc tcgaacaccc ggtgggacgc cggcaggttg ccgaccaccg tcggctcgac
    10741 gtagtagccg cgggcgaggt cgccgtcgga gaggtgctcc ccgccggtgt agaccttgcc
    10801 gtcgcgccgg gcctcggcga ccgcctgctg atggcggtcg accgccttct ggtcgacgac
    10861 cgggccgagc caattctcgc gctggatcgg gttccccacc gtgagcttct cggtcttctc
    10921 gacgagcagc cggacgagct cgtcgtggac cggcttctcg acgtagacgc gcgagttcgc
    10981 ggagcacttc tggccgccga agccgaatgc ggaccgaagg atgccctcgg ccgcctcgtc
    11041 gaggtccgcc gagcgggcga cgatcgccgg gttcttgccg cccatctcga cgatggtcgg
    11101 acgcggccag gtcgacgaga aggaccggta cagccgcatc ccgacctcgt acgagccggt
    11161 gaacacgatg ccgtcgatgc ccgggttcga ctggagctcc tcgccgaccg tgtcgcc
//
LOCUS       DATBSW010000150         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_401349, whole genome shotgun sequence.
ACCESSION   DATBSW010000150 DATBSW010000000
VERSION     DATBSW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401349"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..993
                     /locus_tag="VIF63_04155"
     CDS             145..993
                     /locus_tag="VIF63_04155"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY8238608.1"
                     /translation="MTEALGRIGFAVSRYNPEAKAVLVRGRAWCENQGIATWDAVADD
                     EDKLASELPGSDLVVVLGGDGTFLRVARIIGDSEVPALGVNLGRVGFLAKVEPDELEP
                     ALDGIAAGRHRIAERMRLGATIHPRDGEPTTAACLNEVAVARAAQVRLIQVEVEVSGS
                     HLATYLADGVVVATPTGSTAYSYSAGGAVLDPRLRNLVITPVAAYLSALHSVVTGEDH
                     LIRLHLREAFGGAVVSFDGQRDVPVEVGEWVEVRALPVPLRLVEPEGSTPFYDLLRTK
                     ASLLPD"
     gene            1012..1908
                     /locus_tag="VIF63_04160"
     CDS             1012..1908
                     /locus_tag="VIF63_04160"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HEY8238609.1"
                     /translation="MTTAAADPRLTAAIGEFLLELRVERGLSPLTIAAYRRDLDQFAA
                     GSGPGWRDDPAVVGEFVARLRRQGRRASTQARKVAAIRSFYGFARREGVIERDLADLI
                     DPPRPGRYLPEVLAPDEVERILAAPGDDPAGLRDAAILELLYACGLRVSELTGLDLDR
                     LNLGALEVRVIGKGNRERRVPMGEPARDRLHRYLSGPRQAWTAGKPTPAVFVSQRGTR
                     LGRESVWRLVRRWSQAAGVVGDITPHTFRHSFATHLLEGGADLRVVQSLLGHASISTT
                     QLYTHLTGERVREVYARAHPRA"
     gene            1917..>2437
                     /locus_tag="VIF63_04165"
     CDS             1917..>2437
                     /locus_tag="VIF63_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY8238610.1"
                     /translation="MSYARKLLARGEDVVLESHQHWFVVIGRSWWAIVLAIFALAVLI
                     FVASPPEEGTDSAAEVVAFIFLLIAFARIGWVIWDWRNNEFLVTTRRIVRAEGILNKR
                     MVETSLEKVNDAVLTQSMFGRLFGFGDLDILTAADEMGGIEDFPMVADPVEFKVAMMN
                     QKEAVERPDIAPQA"
BASE COUNT          332 a          843 c          882 g          380 t
ORIGIN      
        1 gcgcgcgctc atcgcggccg ggctggaccg ctcgtacgcg gcgctcaccg agtccgaggc
       61 cgcctccgat ctggcggccg agctcgccgc gctccgtgag cggatcgagg acatcgccac
      121 cagcctcgcc gccgatcgcc cctcgtgacc gaggccctcg ggcggatcgg cttcgcggtc
      181 agtcgttaca acccggaggc caaggccgtc ctggtgcgcg gtcgcgcgtg gtgcgagaac
      241 cagggaatcg cgacctggga cgccgtcgcc gacgatgagg acaagcttgc gtccgagctg
      301 ccggggagcg acctggtcgt cgtcctgggc ggggacggca cgtttctccg cgtcgcgagg
      361 atcatcggcg actccgaggt gccggccctc ggtgtgaacc tcgggcgggt tggcttcctg
      421 gccaaggtcg agcccgacga gctggagccg gcgctggacg ggatcgcggc cggccgccac
      481 cggatcgcgg agcggatgcg actcggcgcg acgattcacc cccgagacgg cgagccgacc
      541 acggcggcct gcctgaacga ggtggccgtg gcccgcgccg cgcaggtgcg gctcatccag
      601 gtcgaggtcg aggtgagcgg cagccatctg gcgacctacc tcgccgacgg cgtcgtcgtc
      661 gccacgccga ccgggtcgac cgcgtactcg tattcggccg gcggggcggt cctcgatccg
      721 cggctgcgga acctcgtgat cacgccggtg gccgcctacc tgtccgccct tcacagcgtg
      781 gtgaccggcg aggatcacct cattcgactc cacctccgtg aggcgttcgg cggggcggtc
      841 gtctcgttcg atgggcagcg cgacgtcccg gtcgaggtcg gggagtgggt cgaggttcgc
      901 gccctgcccg tgccgttgcg cctggtcgag cccgagggca gcaccccgtt ctacgacctg
      961 cttcggacga aggcgtccct gctccctgac tgatatgtct ggtccacgcc catgaccacc
     1021 gccgcggccg accctcgcct gacggccgcc atcggcgagt tcctgctcga gctgcgggtg
     1081 gaacgcgggc tctcgccctt gaccatcgcc gcctaccgcc gcgacctcga tcagttcgcg
     1141 gccggctccg ggccgggctg gcgcgacgac ccggcggtgg tgggcgagtt cgtggcgcgc
     1201 ctccggcggc agggccgacg ggcgtcgacc caggcccgca aggtggcggc gatccggagc
     1261 ttctacggct tcgcccggcg agagggcgtg atcgagcggg atctcgccga tctcatcgac
     1321 ccgccccgcc ccggtcgcta cctgcccgag gtgctcgcgc cggacgaggt cgaacggatc
     1381 ttggccgccc cgggtgacga cccggccggg ctacgagatg cggccatcct cgagctgctg
     1441 tacgcgtgtg gtctgcgggt gtccgagctg accgggctcg acctcgaccg gctgaacctg
     1501 ggtgcgctcg aggttcgagt catcggcaag gggaatcgcg agcgccgggt gccgatgggc
     1561 gagcctgccc gcgaccggct ccatcggtat ctgtccggcc cgcgtcaggc ctggacggcc
     1621 ggcaagccga cgccggcggt gttcgtgtcg cagcgcggaa cgcggctcgg ccgggaatcg
     1681 gtctggcggc tggtgcgacg ctggagccag gcggcggggg tcgtgggaga catcaccccg
     1741 cacacattcc ggcacagctt tgcgacccat ctcttggagg gaggcgccga tctgcgggtg
     1801 gtccagtcgc tcttgggcca tgctagcatc agcactaccc agctctatac ccacctgacc
     1861 ggcgagcgcg ttcgggaggt gtacgcccgg gcgcacccgc gagcctgagg agaccgatga
     1921 gttacgcgcg caagctgctg gcccgaggcg aggacgtcgt cctggagagc caccagcact
     1981 ggttcgtcgt catcggccga tcgtggtggg cgatcgtgct cgccatcttc gccctggcgg
     2041 tcctgatctt cgtggccagc ccacccgagg agggtaccga cagcgcggcc gaggtcgtgg
     2101 ccttcatctt cctgctcatc gcgttcgctc gcatcggatg ggtgatctgg gactggcgga
     2161 acaacgagtt cctggtcacc acccgccgga tcgtgcgcgc cgaggggatc ctgaacaagc
     2221 ggatggtcga gaccagcctc gagaaggtga atgacgccgt gctcacccag agcatgttcg
     2281 ggcggctgtt cggtttcggc gacctcgaca tcctgaccgc cgccgacgag atgggcggca
     2341 tcgaggactt cccgatggtc gccgacccgg tcgagttcaa ggtcgcgatg atgaaccaga
     2401 aggaagcggt cgagcggccg gacatcgccc cgcaggc
//
LOCUS       DATBSW010000151         5865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_48767, whole genome shotgun sequence.
ACCESSION   DATBSW010000151 DATBSW010000000
VERSION     DATBSW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48767"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VIF63_04170"
     CDS             complement(<1..1171)
                     /locus_tag="VIF63_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238611.1"
                     /translation="MESQTDHLEGDRARATRFGGLPTGLVIPLFGAGIFVAAGLVFLL
                     EPMIAKALLPTFGGAPQVWTAATVFFQAALLAGYGYAHLSTTRFGPRRGPLLHLALLA
                     APVLLLPIAIRGGALVEGAPPALAVVGILALSIGAPFIVASATSPLLQRWLSTIGHPG
                     FEDPYFLYAAGNAGSLIGLLAYPFIVEPRFTVREQAILWSAGYIVFALLVGTVGAVVA
                     RTARASPAAGVVDRATDEAILRGVSSLDRRRQLRWIVRAFIPASLLLGVTNHLQTDIA
                     AVPLLWVIPLAIYLLTFVLAFARRTLLPPRTAAALLPLAAVAILFTFLYAAAIPTPVL
                     MAIHYGTFFIVAMLSHGQLAQDRPGAEHLTRFYLLLAVGGALGGLFNAIVAPQLFD"
     gene            1328..3385
                     /locus_tag="VIF63_04175"
     CDS             1328..3385
                     /locus_tag="VIF63_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8238612.1"
                     /translation="MTTEGVSRRRRIAVGAIVVVIAALAACTPKPRPSPSSVPSPTVA
                     PPTPAAVVPSPSATVAPSVAPSTPPSDTPIPGGWTLVKSAPCPDSDFECVTLAVPRDH
                     FIANGPTWEVTFGILHATGKKKGTFVTASGGPGYSGLSVADSYTSAFPEGITDVYDVV
                     FFDQRGIGMSRPFQCPNATGEYYDVDADPADPAGATAFKAAAESYVTRCITETGADPA
                     DLPYFATRQAVEDLEAFREYLKVDKVDLYGESYGTQYAQMYATSHPDRIANLYLDGPV
                     DLTLDGASFYAEAARAFDDDLIWTLTACAADKACAADFEGVSPLDVYDELASKLSSGP
                     ISYDFIKADGSTEARTFTQADLATVAVSYLFGPFDRMMLVRALAAAVDGDYTPLARAA
                     AISIALDPETLAVVPDPTYSDAMYYAVECQDYVYFPDAGDLPAQADAYLGFGHAAGLD
                     PLRLDALFYQDMPCIYWPNRPTVDPRPAPIVDAPYRTFVLVATADPITPVANAHRLVS
                     RLSDVYLILTEGGPHVTFGWGEACPDELIGAYMVKGKRPASRITTCANVIADDYIAVG
                     RDAAADYADALALMSSMDDQVTYTNDYQYQLDAEPLAVGCDFGGSITYTPTDTGTDLA
                     LDACAFTDDVAMTGSGAFDDDSGGLTLDVRFRDGVLRYTRSGDYELHVTGTFRGAKVD
                     LTG"
     gene            complement(3387..4733)
                     /locus_tag="VIF63_04180"
     CDS             complement(3387..4733)
                     /locus_tag="VIF63_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY8238613.1"
                     /translation="MSLAAAGLGSLSAPSPVAAATCTGFMRTQVCNPDVSPNGGTTST
                     TFTFTVVYQDSAVPPRKPCEVQVTIEDVGTFDMTPACSSVSDSDINDGTTFTYKTKLP
                     AGSHSFSFTGGHGSTWRTAGGGEPVTVSAPTPAPTATPKPTPKPTPKPTPKPQATPRP
                     TPKPTPKSAKPTPKPAKPTPKPTSKPARSAAPAAASSRSAEPDGAAVASPTDGAVGIG
                     GIGQVSGNPDGNSDLGGSGRLPPWIPASTIVLGIGLASFAFAWRRRRRRSEPVLASPD
                     RAFETAPIEGFLPAFGGGALDVPAEEAAIPRWRRPSLREARFASDIRPRELREKRTFE
                     APPSSDIERLIVRYDLVSLLGSPDDGRARPVADLRSGDELDVLSRRDDWVHVQTPSGV
                     TGWISTSFVATIPPVDAPPAPKATRPKKSTKPRKATARTSARPTPRPSRSAGLSQG"
     gene            5143..5850
                     /locus_tag="VIF63_04185"
     CDS             5143..5850
                     /locus_tag="VIF63_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8238614.1"
                     /translation="MMNVRDVMTRSVVSVEQGTPLKDVAHFLIDNGISGVPVVDADGA
                     VLGVVSEADLLIKEQGADAIHHRPLARFLGESAEARAQVVKLRAITAGEAMSAPAITI
                     TSARSINEAAAIMTSRHVNRLPVVDDGRLVGIVSRADLVRAYIRSDEELGATIRKNVI
                     LGILWLDPALFSVAVKDGVASIGGQVERRSTAEMIERAVRMVPGVVDVHASVSWSMDD
                     RTLEPADLDPVFPFGPR"
BASE COUNT          931 a         1980 c         2065 g          889 t
ORIGIN      
        1 gatcgaagag ctgcggcgcc acgatcgcgt tgaagagccc gccgagggcg ccgccgacgg
       61 ccagcaacag gtagaagcgc gtgaggtgct cggcgcccgg gcgatcctgc gcaagctggc
      121 cgtgactgag catcgccacg atgaagaacg tcccgtagtg gatcgccatc aagacgggcg
      181 tcggtatggc tgcggcgtac aggaacgtga acaggatcgc gaccgccgcg agcgggagca
      241 gggccgccgc ggtacgcggg ggcagcagcg ttcgcctggc gaaggccagc acgaacgtga
      301 gcaggtagat cgcgagcggg atcacccaga gcagcgggac ggcggcgatg tcggtctgca
      361 gatggttcgt gacgccgagg agcaggctcg ccgggatgaa tgcccggacg atccatcgaa
      421 gctggcgtcg gcggtcgagg gacgaaacgc ccctgaggat cgcttcgtcg gtcgctcgat
      481 ccacgacgcc cgcagccggg ctggctcgcg cggtgcgagc gacgaccgcc ccgacggtcc
      541 ccaccaggag ggcgaacacg atgtagccgg ccgaccacag gatcgcctgc tcgcggaccg
      601 tgaaccgcgg ttcgacgatg aacggatagg cgagcagccc gatcaggctg ccggcgttcc
      661 cggccgcgta gaggaagtac gggtcctcga agccggggtg cccgatggtc gacagccagc
      721 gctgcagcag cgggctcgtc gccgacgcga cgatgaacgg agcgccaatg ctgagcgcca
      781 ggatcccgac cacggcgagc gcgggcggcg cgccctcaac cagggcgccg ccgcggatgg
      841 caatcggcag caggagcacc ggtgcggcga ggagcgccag gtggagcagc ggccctcggc
      901 gcggcccgaa ccgggtggtc gataggtgcg cgtagccgta gccggcgagc agggctgcct
      961 ggaagaacac cgtcgcggcc gtccacacct gcggtgcgcc gccgaacgtc ggcagcagcg
     1021 ccttggcgat catcggctcg agcaggaaca ccaggccggc ggcgacgaag atgcccgcgc
     1081 cgaacagcgg gatcaccaag ccggtcggca ggcccccgaa gcgcgttgcg cgcgcgcggt
     1141 cgccctcgag gtggtccgtc tgcgactcca tcgcggggat gatgccggaa cccgagtctg
     1201 gggaatggct gccgaggaac gattcgaacc ttcccgccgc catcgcgaga atagcgggcg
     1261 ctctcgcggg tacaggcggc gcccgctagc tcgtcgagcc caagagagcc tcggtatgat
     1321 ggccgggatg acgacggagg gagtcagccg ccgccggcga atcgctgtcg gcgccatcgt
     1381 cgtcgtcatc gcagcgctcg cggcctgcac gccgaagcct cgcccgagcc caagctcggt
     1441 ccccagcccg acggtcgcac cgccgacccc tgccgccgtg gtcccctcac cctccgcgac
     1501 ggtggctccg tcggtggcgc cctcgacccc gccgagcgac acgccgatcc ccggcgggtg
     1561 gacgctcgtg aagtcggcac cgtgcccgga cagcgacttc gagtgcgtca ccctcgcggt
     1621 cccgcgcgac cacttcatcg caaatggccc gacgtgggag gtgacgttcg ggatcctcca
     1681 tgcgaccggc aagaagaagg gcaccttcgt caccgccagc ggcgggccgg gctacagcgg
     1741 actctccgtc gcggacagtt acaccagcgc cttccccgag ggcatcaccg acgtttacga
     1801 cgtcgtcttc ttcgaccagc gcggcattgg gatgtcgcgt ccgttccagt gcccgaacgc
     1861 gacgggggag tactacgacg tcgatgccga tcccgcggat cccgcaggtg cgaccgcctt
     1921 caaggccgcc gccgagtcct acgtcacccg gtgcatcacc gaaaccggtg cggacccggc
     1981 ggacctgccg tacttcgcga cgcgtcaggc cgtcgaggac ctggaggcct tccgcgagta
     2041 cctgaaggtc gacaaggtcg acctctacgg cgagagctac ggcacgcagt acgcgcagat
     2101 gtacgcaact tcccacccag atcgcattgc gaacctgtac ctcgatggcc cggtcgacct
     2161 cacgctcgac ggcgcgtcct tctacgcgga ggccgcgcgg gcgttcgacg acgacctcat
     2221 ctggaccctg accgcatgcg ctgcagacaa ggcgtgcgcc gccgatttcg agggcgtatc
     2281 cccgctcgat gtgtacgacg agcttgcctc gaagctgtcg agcggcccga tctcctacga
     2341 cttcatcaag gccgacggca gcacggaggc acggacgttt acccaggcgg atcttgccac
     2401 cgtggccgtg tcctaccttt tcgggccgtt cgaccggatg atgctcgtgc gggcgctggc
     2461 ggccgcagtc gatggcgact acacgccgtt ggcgcgagcg gcggcgataa gcatcgcgct
     2521 cgatccagag acgctcgccg tcgtgcccga cccgacctac tcggacgcga tgtactacgc
     2581 ggtcgagtgc caggactacg tctacttccc cgatgccggc gacttgcccg cgcaggcaga
     2641 cgcctacctc gggttcggcc acgccgccgg gctcgacccg ctacgcctcg acgcgctgtt
     2701 ctaccaggac atgccgtgca tctactggcc gaatcggccg acggtcgatc ctcgtccggc
     2761 gccgatcgtc gacgcgccat accggacatt cgtcctcgtc gcgaccgctg acccgatcac
     2821 cccggtcgcg aacgctcacc gcctggtgag tcggttgagc gacgtgtacc tgatcctcac
     2881 cgagggcgga ccgcacgtga cgttcggctg gggcgaggcc tgtcccgatg agctcatcgg
     2941 cgcctacatg gtcaagggca agcgcccggc ctcgcggatc accacctgcg ccaacgtgat
     3001 cgcggacgac tacatcgccg ttggtcgaga cgctgcggcc gactacgccg acgcgctcgc
     3061 cctgatgtcg tcgatggacg accaggtgac gtacaccaac gactaccagt accagctcga
     3121 cgccgagccg ctcgcagtcg gctgcgactt cggcgggtcg atcacctaca cgcccaccga
     3181 caccgggacg gacctcgcgc tcgacgcgtg cgccttcacg gacgacgtgg cgatgacggg
     3241 ctcaggcgcc ttcgacgacg actctggcgg cctgacactc gacgtgcggt tccgggacgg
     3301 cgtgcttcgg tacacccgat cgggcgacta cgagctgcac gtgaccggga ccttccgggg
     3361 tgccaaggtc gacctgaccg gctaggtcag ccctggctga ggccggcgga tcgggaaggc
     3421 cggggcgtcg gtcgcgccga cgtccgggcg gttgccttcc ggggcttggt gctcttcttc
     3481 gggcgcgtcg ccttcggcgc cggcggcgca tcgaccggcg ggattgttgc cacgaacgac
     3541 gtggagatcc agcctgtcac cccgctcggc gtttggacgt ggacccaatc gtcgcgccgg
     3601 gaaagcacgt cgagctcatc gcccgatcgg aggtcggcga cggggcgggc gcgaccatca
     3661 tccgggctgc cgaggagcga caccaggtcg tagcggacta tcaatcgctc gatgtccgat
     3721 gacggcgggg cctcgaaggt gcgtttctcg cgcagctcgc gcggtcggat gtccgacgcg
     3781 aagcgcgcct ctctgagcga cggtcgacgc cagcgtggga tggccgcctc ctccgccggg
     3841 acgtcgagtg cgccgcctcc gaaggctggg aggaacccct cgatcggtgc cgtctcgaac
     3901 gcgcggtcgg gcgatgcgag cacgggctcg ctccgccgcc ggcgccggcg ccaagcgaag
     3961 gcgaaggagg ccaacccgat cccgaggacg atcgtcgagg cgggaatcca gggcggcagc
     4021 cggccggatc cgccgagatc actgttccca tcgggattac cggagacttg cccgatccct
     4081 ccgatgccga ccgcgccatc ggtcggcgac gccacggcgg caccgtctgg ctcggctgag
     4141 cgcgagctcg cggcggcggg cgccgcgctg cgcgctggct tcgacgtcgg cttgggcgtc
     4201 ggctttgcag gcttcggagt gggcttcgcg gacttcggcg ttggcttcgg cgttggccga
     4261 ggcgtcgcct gcggctttgg cgtcggcttg ggcgtcggct tgggcgtggg cttcggggtc
     4321 gcggtcggcg ccggtgtcgg cgcggagacc gtgaccggct ccccgccgcc ggccgtgcgc
     4381 caggtcgagc catggccacc ggtgaacgag aacgaatgcg accccgccgg gagcttcgtc
     4441 ttgtaggtga acgtcgttcc gtcattgatg tcgctgtctg acacggacga gcacgcgggt
     4501 gtcatgtcga aggtgccgac atcctcgatc gtcacctgca cctcgcacgg cttgcgaggg
     4561 ggaactgccg aatcctggta gacgacggtg aaggtgaagg tcgtcgaggt cgtcccgccg
     4621 ttcggggaga catcggggtt gcacacctgg gtcctcatga acccagtgca cgtcgcggcg
     4681 gcaacgggcg agggcgcgct gaggctgccg aggccggccg ctgccaggct cacggcaaat
     4741 agcgtgcgcg cgacctgcca tttcactggc cggatcgtgc aggcacggat cggccacgtc
     4801 cgtgacccat cagtcatggt ccgagatggg tcgatccgcc ggacacgggc cagcgggaca
     4861 cggcgctcga atggggaagt tggctggcga ggaaggattc gaattcggtc cgagtgcctc
     4921 ggcgcgttcg ggtcaaggcg gaggtcctgc atctggctcg ggacctccgc cgttcatgac
     4981 gcgggcggcc gcggggaaac gttgcgcccg gccccgcaac gtatccacga ggaacgggcc
     5041 atcgcattcc atccctggtg gcccctgagg acgggacctt cgaccctgtc gacccgggtg
     5101 cgacgaggac aacgtagcca tgaaggcgag ggtggttttc gcatgatgaa cgttcgcgac
     5161 gtgatgaccc gctcagtggt ctcagttgaa caggggaccc ctctcaagga tgtcgcccac
     5221 ttcctgatcg acaacgggat cagtggggtt ccggtcgtcg atgcggatgg cgcggtcctg
     5281 ggcgtggtgt ccgaggctga cctgctcatc aaggagcagg gcgccgatgc gatccaccac
     5341 cggccactcg cacgcttcct gggcgagtct gccgaggcgc gtgcccaggt cgtgaagctt
     5401 cgagccatca ccgccggcga ggcgatgagc gctccggcga tcacgatcac gtcggcccga
     5461 tcgatcaatg aagccgccgc gatcatgacg tctcgacacg tcaaccgtct gccggtcgtg
     5521 gacgatggac ggctcgtcgg catcgtcagc cgcgccgatc tggttcgcgc gtacattcgc
     5581 tcggacgagg agctcggcgc gacgatcagg aagaacgtga ttctcgggat cctgtggctc
     5641 gatccggcgc tgttctcggt cgccgtcaag gacggcgtgg cctcgatcgg tgggcaggtt
     5701 gagcgacgct cgacggccga gatgatcgag cgcgcggtga ggatggttcc gggggtcgtc
     5761 gacgtccacg cgtccgtgtc ctggtcgatg gatgaccgga ccctggagcc ggccgacctc
     5821 gatccggtgt ttccgttcgg gcctcgatag cgtcgagtcc ctgca
//
LOCUS       DATBSW010000152         4970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1071321, whole genome shotgun sequence.
ACCESSION   DATBSW010000152 DATBSW010000000
VERSION     DATBSW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071321"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VIF63_04190"
     CDS             complement(<1..561)
                     /locus_tag="VIF63_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY8238615.1"
                     /translation="MPRFNYGGQALIEGVLMRGRDAIAVAFRHPDGHIVWASEPLTSG
                     PHGWRLAKAPFVRGLIVLYETLVVGTRWLIRSANIQASAEGVELGKGSVALLLGVTLL
                     LGVGIFFLLPLFIAAFTTAAITQDWVQHLVEGLVRVAIFLGYLAVISRAPDIRRVFEY
                     HGAEHMTIHALEAGDPLTTEAVRKYPT"
     gene            complement(605..817)
                     /gene="rpmE"
                     /locus_tag="VIF63_04195"
     CDS             complement(605..817)
                     /gene="rpmE"
                     /locus_tag="VIF63_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY8238616.1"
                     /translation="MKPDIHPKYFDAKVHCGSCGREWTVGSTRAELRVDVCGNCHPFY
                     TGTQNIIDTAGQVERFQKRLERQVRA"
     gene            complement(814..1092)
                     /gene="rpmA"
                     /locus_tag="VIF63_04200"
     CDS             complement(814..1092)
                     /gene="rpmA"
                     /locus_tag="VIF63_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY8238617.1"
                     /translation="MAHKKAGGSVRNGRDSVGQRLGQKAGDGQFVKAGTIIVRQRGMT
                     FLAGPGTDLGGDYTLFATTAGKVKFEFATKSKKRVRVIPEATEAANPA"
     gene            complement(1096..1758)
                     /gene="rplU"
                     /locus_tag="VIF63_04205"
     CDS             complement(1096..1758)
                     /gene="rplU"
                     /locus_tag="VIF63_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY8238618.1"
                     /translation="MYAVIETGGKQYRVEVGTELEIELLDAGAGESVAFERVLLVADG
                     EAASIGRPVVENASVSAEVLRRDRGEKVISFKYRPKARRRVKKGHRQELLIVRISDIV
                     LDGKSAAKDLAKVEEQRKTERQRLQEAARKQAEQDSALAAKLAADAAEADAADEAAKP
                     AKGAKAKAAPATKTSAKAATTKAATKTSSTKASAKADTTDKGAAKAAKSTKTKASGTE
                     KG"
     gene            2613..2837
                     /locus_tag="VIF63_04210"
     CDS             2613..2837
                     /locus_tag="VIF63_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238619.1"
                     /translation="MASRPTALRVRWLRFTGTISYGLYLYHYPIGAALALDLPVLVRA
                     VVLGVLTFGAAIASWFWIERRFLRHREHRA"
     gene            complement(3119..4279)
                     /gene="rodA"
                     /locus_tag="VIF63_04215"
     CDS             complement(3119..4279)
                     /gene="rodA"
                     /locus_tag="VIF63_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY8238620.1"
                     /translation="MSIVSARSSRPSRWTAARVSVVWRSFDLQLVVYAALLLCFGLAM
                     AYSNTATAGLGVFDGGSVFLRALMWTAIAVAVFLVATSFDYHWLKTFAWPLYLIQLGL
                     LVLTLAIGGGVGGTSRWVSLFGLQFQFSELAKVLMIVVLANYLGSRVGKLGSLWAMVG
                     AGMLAGPPFVLVLMQPDLGTALVFGAILVATLFMSGASLRWLGIMAGAAIAAFPFVWT
                     YILADYQKARLTAFLNPLADIQGAGYQLYQAQIAVGSGGWFGKGLTNGAQNALGLLPV
                     QDTDFVFAILAEELGFVGAIVVLGLFVALLWRVLSAGWRSKDPFGTIFACGVGAMLLF
                     QLFVNVGMVIGIMPITGIPLPFISHGGASLISIALALGILQSINIRQGRAEW"
     gene            complement(4292..>4970)
                     /locus_tag="VIF63_04220"
     CDS             complement(4292..>4970)
                     /locus_tag="VIF63_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8238621.1"
                     /translation="FGARTQIDLPAEASGIVPTNQWKQDTLGLPIYPGETYLAGIGQG
                     YVAVTPIQLLNAYCALANGGTLYKPHVVGEVIGADGAVHAVEPEVIGEVDAPDNVLRV
                     MRRAGRNMVVLRHTYNLVDMPIVIAGKTGTAQFSAKEKNLPFHSWFAGFVPKDPWTKD
                     SDPHGWKAVQRTDSELAVLVLAYDSGTRGNAATEIAKYFFQLHFNIKKDYRNFDLLKR
                     RSFLPSN"
BASE COUNT          782 a         1639 c         1721 g          828 t
ORIGIN      
        1 ggtcgggtac ttgcgcaccg cttcggtcgt cagtgggtcg ccggcctcga gcgcgtggat
       61 ggtcatgtgc tcggcgccgt ggtactcgaa cacgcgccgg atgtccggcg cgcgcgagat
      121 caccgcgagg tagccgagga agatcgcgac gcggacgagg ccctcgacaa ggtgctggac
      181 ccagtcctgc gtgatggccg cggtcgtgaa ggcggcgatg aacagcggca ggaggaagaa
      241 gatcccgacg ccgagcagga gcgtgacgcc aagcaggagc gccaccgagc ccttgccgag
      301 ctcgacaccc tctgcgctcg cctggatgtt tgcgctccgg atgagccagc gcgtgcccac
      361 gacgagcgtc tcgtacagca cgatcaggcc gcgcacgaag ggcgccttgg cgaggcgcca
      421 gccgtgcggg ccggaggtca gcggctcgga cgcccagacg atgtgcccgt cggggtggcg
      481 gaaggcgacc gcgatcgcgt cgcgaccgcg catgaggacg ccctcgatca gcgcttggcc
      541 gccgtagttg aagcgaggca tcgtcggagt ctaccggctc gctgtcgcga tccctgtgtg
      601 ggagctaggc gcggacctgc cgctccaggc gcttctggaa ccgctcgacc tggccggcgg
      661 tgtcgatgat gttctgcgtg cccgtgtaga acgggtggca gttgccgcac acgtcgacgc
      721 gcagctcggc tcgggtcgag ccgaccgtcc actcgcgccc gcaggagccg cagtggacct
      781 tggcgtcgaa gtacttggga tggatgtcgg gcttcatgcc gggtttgctg cctcggtcgc
      841 ctcggggatg acccgaacgc gcttcttgga tttcgtcgcg aactcgaact tgaccttgcc
      901 ggctgtcgtc gcgaacaggg tgtagtcgcc gccgaggtcc gtgcccggac cggcgaggaa
      961 ggtcatgccg cgctggcgca cgatgatcgt gcccgccttc acgaactggc cgtcaccggc
     1021 cttctggccg agccgctgcc caacgctgtc ccggccgttc ctgacggacc cgccggcctt
     1081 cttgtgtgcc atcgatcagc ccttctccgt gccggatgcc ttggtcttcg tcgacttggc
     1141 ggccttcgcg gcccccttgt cggtggtatc ggccttggcc gacgccttcg tgctcgatgt
     1201 cttcgtcgct gccttggtcg tcgcagcctt ggcgctggtc ttcgtcgcag gcgcagcctt
     1261 ggccttggcg cccttggccg gctttgctgc ctcgtcggcg gcgtcggctt ctgcggcgtc
     1321 ggcggcgagc ttcgcggcga gggccgagtc ctgctccgcc tgcttgcggg ccgcttcctg
     1381 gagccgctgg cgctcggtct tgcgctgctc ctcgaccttc gcgaggtcct tcgctgcgct
     1441 cttgccgtcg agcacgatgt cgctgattcg gacgatcaac agctcctggc gatggccctt
     1501 cttgacgcgg cgacgcgcct tgggccggta cttgaagctg atgaccttct cgccgcggtc
     1561 gcggcggagg acctcggccg agacgctcgc gttctcgacg accgggcgcc cgatcgaggc
     1621 agcctcgccg tcggcgacga ggagcacgcg ctcgaacgcg acggactcgc cggcgccggc
     1681 atcaaggagc tcgatctcga gctcggtgcc gacctcgacg cgatattgct ttccgccggt
     1741 ctcgatgact gcgtacatgg gacccctggt gtcgatctga tttcgcgcgg cagcacgccg
     1801 caaccttgtg ggcaggaagg gatgccgcgg caagccgggg caagatcggg gattgtagag
     1861 ccagccgccg ttgagcgtca atcgaccggc ggccggggta gtgggtcagt ttgagtcgcg
     1921 tggaccctga cgcagcatca tgagtcgcgc ccaagccatc ggcagcagga cgtacacgcc
     1981 ccacgcgatg aacccgatgg cgagtatctg ttgggctgga tcgggcggca agaccggccc
     2041 gggggtcggg tctggttgcc acaggatggc catcccgacg actacgagaa tgcccgcaaa
     2101 ctgtgcccgc gtcagggggt gtcgggtctc gggccgaatg gcccgaggaa gccaatcgag
     2161 acctttaggc aatcgtgttg ggtcatcccg cgggtcggac ataccgagag agtaagccca
     2221 tgtgatgaac gtgttcatgc cggggatgga ccacacgtgg tcgctcgccg tcgaggagca
     2281 gttctacatc ctctggccac tcgtcctgct ggtctcgatc ggccgcggct ggtccatcga
     2341 gcgtgtcgtc tgggtcctga tcggcgtgtc ctacgggacc gccatgttcg gctcggacct
     2401 cctggcaatc cacgggtctc ccgagcgtgc gaaggacatc ctgctcggcg cagcgatcgc
     2461 tctcgcgtgt cgtcggttgg gccgggatgt catgccgtcg acgctgacca cgaccacggc
     2521 cgcgatcgcg cttgcgatgg catgcctggt cccgtccacg ttgacgaccg cgctgctgat
     2581 cccgatccca agccgccgtg atcgtggctt cggtggcaag ccggccaacc gcattgcgag
     2641 tgcgctggct ccgcttcacc ggcacgatca gctacgggtt gtacctctac cactacccga
     2701 tcggggctgc gctcgccctc gatctgcctg tgctcgtacg agcggtggtc ctcggcgtcc
     2761 tgacgttcgg cgccgcgatt gcctcgtggt tctggatcga gcgtcggttc ctgcggcacc
     2821 gggagcaccg cgcttgaggg cagaatcacg aaacccgcgg agccgagcca tgtaggggcc
     2881 ggtgccgacg ggtttctcac ccccaacgta gcgggcgacg tccgccgcgc gacgtgccgt
     2941 tcggccctta tcgcagcgta ggtggcacgg tggtgcgcgg ctgcgacggc acggtcagta
     3001 cgtgccgacc aacggaatca gcccggcgct cacggcgacg acgcccagca cgaagccgat
     3061 cacgagcacg acgatgagaa ggaatgcccg cttggccatc agcgcggcag tcgcgacgtc
     3121 accactcggc gcgaccctgg cggatgttga tgctctgcag gatcccgagc gccaatgcga
     3181 tgctgatcag cgacgcgccg ccgtggctga tgaacggcag cggaatgccc gtgatcggca
     3241 tgatgccgat gaccatcccg acgttcacga acagctggaa cagcagcatc gcgccgacgc
     3301 cgcaggcgaa gatcgtgccg aacgggtcct tcgagcgcca gcccgccgac agcacgcgcc
     3361 agagcagggc gacgaacagc ccgagcacga cgatcgcgcc gacgaacccg agctcctcgg
     3421 caaggatcgc gaacacgaag tccgtgtcct ggaccggcaa caggccgagc gcgttctgcg
     3481 cgccattcgt gaggcccttg ccgaaccagc cacccgagcc gacggcgatc tgggcctggt
     3541 agagctggta cccggcaccc tggatgtcgg cgaggggatt gaggaaggcg gtcagccgcg
     3601 ccttctggta gtcggcgagg atgtaggtcc agacgaacgg gaacgccgcg atcgcggcgc
     3661 cggccatgat cccgagccag cggaggctcg cgccggacat gaacagggtc gcgacgagaa
     3721 tcgccccgaa caccagcgcc gtcccgaggt ccggttgcat gagcaccagc acgaacggcg
     3781 gtccggcgag cattcctgcg ccgaccatcg cccagagcga gccgagcttc ccgactcgac
     3841 tgccgaggta gttggcgagc accacgatca tcagcacctt ggcgagctcg ctgaactgga
     3901 actggagccc gaacagcgag acccagcgcg aggtgccgcc gacgccgccg ccgatcgcga
     3961 gcgtcaggac gagcagcccg agctggatca ggtacagcgg ccatgcgaac gtcttgagcc
     4021 agtggtagtc gaacgacgtg gcgacgagga agacggccac cgcgatcgca gtccacatca
     4081 gcgcgcgcag gaacacggag ccaccgtcga acacgcccag gccggcggtg gcggtgttgc
     4141 tgtaggccat cgcgaggccg aagcacagca ggagcgccgc gtacacgacg agctggaggt
     4201 cgaacgagcg ccagaccacg ctcacgcgtg ccgccgtcca gcgggatggt cgtgacgagc
     4261 gcgcgctgac gatgctcacg gtcgaatcgt cctagttgct cggcaggaag ctgcggcgct
     4321 tgaggaggtc gaagttccgg tagtccttct tgatgttgaa gtggagctgg aagaagtact
     4381 tcgcgatctc ggtggcggca ttgccgcgcg tgcccgagtc ataggcgagc accaggaccg
     4441 cgagctccga gtcggtccgc tggacggcct tccagccgtg cggatcggag tccttcgtcc
     4501 acgggtcctt gggaacgaag cccgcgaacc aggagtggaa cggcaggttc ttctccttcg
     4561 cgctgaactg cgcggtacct gtcttgccgg cgatcacgat cggcatgtcg accaggttgt
     4621 acgtgtggcg gagcacgacc atgttccggc cggcgcggcg catgacccgg agcacgttgt
     4681 cgggcgcgtc gacctcaccg atcacttcgg gctctaccgc gtgcacggcg ccatccgcgc
     4741 cgatgacttc gccgacgacg tgcggcttgt acagcgtgcc gccgttcgcg agcgcgcagt
     4801 aggcgttgag gagctggatc ggagtgacgg ccacgtagcc ctggccgata ccggccaggt
     4861 acgtctcgcc ggggtagatc ggcaggccca gcgtgtcctg cttccactgg ttggtcggga
     4921 cgatgcccga cgcctccgcg ggcaggtcga tctgcgtccg tgccccgaac
//
LOCUS       DATBSW010000153         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1247919, whole genome shotgun sequence.
ACCESSION   DATBSW010000153 DATBSW010000000
VERSION     DATBSW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247919"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VIF63_04225"
     CDS             complement(<1..783)
                     /locus_tag="VIF63_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY8238622.1"
                     /translation="MTPSATTRFAPAPTGRLHLGHLANAIYVWGLARRFGARVILRIE
                     DHDRQRCRPEFETNLLDDLDRLGIAADEPSTYDLRAGPSSFRQSDNGAAYEAALARLD
                     AVADVYACDCSRATFAAWRSEHRGSWRGPGCPGDCRNRRLERRAEVGLRVALGDGDES
                     WDDLLVGRQSGPVASTGDLLARDRSGNWTYALCVVVDDLRHGVDLVVRGEDLLDATAT
                     QIRLGRLLGRADPPRFAHHPLVRRPDGSKLSKADGATAVGELL"
     gene            complement(780..1127)
                     /locus_tag="VIF63_04230"
     CDS             complement(780..1127)
                     /locus_tag="VIF63_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY8238623.1"
                     /translation="MSTRRPARTSAELARLRAEIDELDRRIVSLLNLRAQLTVAVGHE
                     KAAIGRRAIRDRDREREVLLRVSIHNGGPVPQADLLAIYRRLIASARALEMAERERLR
                     RGEDDGPAEPPGA"
     gene            complement(1138..1575)
                     /locus_tag="VIF63_04235"
     CDS             complement(1138..1575)
                     /locus_tag="VIF63_04235"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY8238624.1"
                     /translation="MTRILVLQGPNLNLLGTREPELYGRETMDQIHAHIGRKALDLGL
                     EVVFFQSNHEGALIDRLHERDFDAAIVNAGGLTHTSVSLRDALLAVEKPFVEVHLTDP
                     STREPFRSVNFLKDIALESIVGHGAAGYEMALEYLVTRVVSRW"
     gene            complement(1572..3227)
                     /gene="aroB"
                     /locus_tag="VIF63_04240"
     CDS             complement(1572..3227)
                     /gene="aroB"
                     /locus_tag="VIF63_04240"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY8238625.1"
                     /translation="MELVVVGLPGSGKTAVGRRVAARHAAAFIDLDEEIERQAGRRIP
                     EVFEAEGEAGFRRREREVIEALGAVDDGPRLARVIAPGGGGIVDPHNRWRLFRGRRIC
                     WLDVRTEVLAQRLRRSPNVRPLIVGRDPMGALRDLMAARGRFYSAATRVIGVAEMASV
                     VEAVDATLKAEAPTGTVLVRAETRLGRLVLGEGILATEVATALAELAATRAILVSEPG
                     AWAAVGERLAADLAAAGLPVEHVLLPQGEAAKRLSVIEDAASELARLRVERREPIVAV
                     GGGALGDAAGFLAATYLRGIPFIQVPTTLVAQIDSSIGGKTGVDLPEGKNLLGAFHQP
                     ASIVIDISVLATLDERQRRAALAEAAKMAALGDEKLFATLEASGAAIATGDHAAVESG
                     ALAEVVERAAWAKVDVVLADERESGARISLNLGHTLGHAIEAAAGFGALLHGEAVAYG
                     LRAAARIGEARGVTTPERAKRIETLLDGLELGKSPTGLDLDEVLGYMATDKKHAGRAL
                     RWVLPTADGYAIDAEVPEPLVREVAAAVLEGTSAGAPAIAARP"
     gene            complement(3231..4517)
                     /gene="aroC"
                     /locus_tag="VIF63_04245"
     CDS             complement(3231..4517)
                     /gene="aroC"
                     /locus_tag="VIF63_04245"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8238626.1"
                     /translation="MTVPFRFLTAGESHGRTLGVTIEGVPAGLALAEADLTSDLARRQ
                     RGYGRGARQQIEHDRAEIVAGVRHGRTLGSPILLLVANKDWENWTRVMQVEPLTDEEA
                     AALLAAAEGGDKRSTPVTRVRPGHGDLAGALKYGHDDVRNVLERASARETAARVAAGG
                     VARAFLKELGIDVWSFTAEVGGVAVDPANATRSREDADASPLRCPDPDAEVAMIARID
                     EARDAGDTVGGVFEVVATGVPIGLGSYVHWDRRLDAALAAAVMSINIVKGVELGLGFE
                     QTRRFGSQVHDVIEGRGEAGRWIHRSNNAGGLTAGVSNGEPIVVRGAVKPISTLARPL
                     PSADLRTGEAVEKAHYERSDISVVPAAGVVGEAMVMLTLAAAVLEKTGGDSMAEVRES
                     MARWRKRLTGAPAGDGATAPKSAPAADDEAASGGDD"
BASE COUNT          638 a         1758 c         1668 g          637 t
ORIGIN      
        1 gagcagctcg ccgaccgcag tcgcgccgtc ggccttgctg agcttcgagc cgtccgggcg
       61 gcggacgagc gggtggtggg cgaaccgcgg cggatccgca cgcccgagga gccggccgag
      121 ccggatctgc gtcgccgtgg catcaaggag gtcttcgccg cggacgacga ggtcgacgcc
      181 atgacgaagg tcgtcgacga ccacgcacag cgcgtacgtc cagttgccgc tccggtcacg
      241 cgcgagtaga tcgccggtgc tcgcgaccgg acctgactgc cggcctacca gcaggtcgtc
      301 ccaggattcg tcgccatcac cgagcgccac gcgcagcccc acctcggcgc gccgctcgag
      361 gcggcggttg cggcagtcgc cggggcagcc agggccgcgc cacgatcctc ggtgctccga
      421 ccgccaggcg gcgaaggtcg cacgcgaaca gtcgcaggca tagacgtcgg cgaccgcgtc
      481 gagccgggcg agggcagcct cataggccgc gccgttgtcg ctctgccgga acgacgacgg
      541 tccggcgcgg aggtcgtacg tcgagggctc gtcggcggcg atgcccaggc ggtcgaggtc
      601 gtcgagaagg tttgtctcga attccgggcg gcagcgctgg cggtcgtggt cctcgatccg
      661 gaggatcacc cgagccccga accggcgcgc cagcccccag acgtagatcg cgttggcgag
      721 gtggccgaga tggagccgcc cggtcggcgc cggcgcgaag cgagtggtcg cgctcggcgt
      781 cacgcgccgg gcggttcggc ggggccgtcg tcctcgcccc ggcgaaggcg ctcgcgctcg
      841 gccatctcga gggcccgcgc cgaggcgatg agccggcggt agatggcgag caggtcggcc
      901 tggggcacgg gcccgccgtt gtggatcgag acgcgcaaca gcacctcgcg ctcgcgatcg
      961 cggtcccgga tggcgcgccg cccgatcgcc gccttctcgt ggcccaccgc gaccgtgagc
     1021 tgggcacgca ggttgaggag cgacacgatg cgccgatcga gctcgtcgat ctccgcccgc
     1081 aggcgcgcga gctcggcgct cgtccgggcc ggccggcggg tgctcatcga acggagctca
     1141 ccaccgcgag acgacccgcg tcacgaggta ctcgagcgcc atctcgtagc cggccgcgcc
     1201 gtgcccgacg atcgactcca gcgcgatgtc cttcaggaag ttcacggacc ggaacggctc
     1261 gcgcgtcgat gggtcggtca ggtggacctc gacgaacggc ttctcgaccg ccaggagcgc
     1321 gtcgcgcagc gacaccgagg tgtgggtcag cccgcccgcg ttgacgatcg ccgcgtcgaa
     1381 gtcgcgctcg tgcaggcggt cgatgagcgc gccctcgtga ttcgactgga agaatacgac
     1441 ctcgagcccg aggtcgagcg ccttgcgccc gatgtgggcg tggatctggt ccatcgtctc
     1501 gcgcccgtag agctccggct cgcgggtgcc gagcaggttc aggttcgggc cctggaggac
     1561 caggatccgg gtcacgggcg agccgcaatc gcgggagcgc ccgcgctcgt accctcgagg
     1621 acggccgccg ctacctcgcg aaccagcggc tccgggacct cggcgtcgat cgcgtagcca
     1681 tccgccgtcg gcaggaccca gcgaagggcc cggccggcgt gcttcttgtc ggtcgccatg
     1741 tagccaagca cctcgtcgag atccaggccg gtcggcgatt tgccgagctc caggccgtcc
     1801 agcagcgttt cgatccgctt tgcgcgttcg ggcgtcgtca ccccacgtgc ctcgccgatt
     1861 ctggctgccg cgcgaagccc gtacgcgacc gcctcgccgt ggagcaacgc gccgaacccg
     1921 gccgccgcct cgatcgcatg gccgagggta tgccccaggt tcagcgagat cctcgcgccc
     1981 gactcgcgct cgtcggcgag cacgacatcg accttcgccc aagcggcgcg ttcgacgacc
     2041 tcggccagcg cgcccgactc gacggccgcg tgatcgccgg ttgcgattgc cgcacccgat
     2101 gcctcgagcg tcgcgaatag cttctcgtcg cccagcgccg ccatcttggc cgcctcggcg
     2161 agcgcggcgc gacgctggcg ctcatcgagg gtcgcgagga cggagatgtc gatcacgatc
     2221 gacgccggct ggtggaacgc gccgagcagg ttcttgccct cgggcaggtc gacgccggtc
     2281 ttgccgccga tcgaggaatc gatctgggcg acgagggtcg tcggcacctg gatgaaggga
     2341 atgccgcgca ggtaggtggc cgcgaggaag cccgccgcat cgccgagcgc gccaccaccg
     2401 accgcgacga tcggctcgcg ccgctcgacc cggagtcgcg cgagctcgct ggccgcgtcc
     2461 tcgatcaccg acaggcgctt ggccgcctcg ccctgtggca ggagcacatg ttccacgggc
     2521 agcccggcgg ccgcgagatc ggccgcgagc cgttcgccga cggctgccca cgcgccgggc
     2581 tcggagacga ggatcgcgcg agtcgccgcg agctcggcga gtgctgttgc aacctcggtc
     2641 gcgaggatgc cctcacccag cacgagcctg cccagccgcg tctcggctcg cacgaggacc
     2701 gtgccggtgg gcgcctcggc cttgagggtc gcatcgacgg cctcgacgac cgacgccatc
     2761 tcggccacgc cgatcacccg tgtcgcggcc gagtagaagc ggcctcgggc ggccatgagg
     2821 tcccgcaggg cacccatcgg gtcgcgcccg acgatcagcg gtcgcacatt cgggctgcgt
     2881 cggagccgct gcgcgaggac ctccgtccgc acatcaagcc aacagattcg ccggccccgg
     2941 aagagccgcc agcggttgtg cggatcgacg atcccgccgc cgccgggcgc gatcacccgg
     3001 gccagacgtg gcccgtcgtc gacggcgccg agcgcctcga tcacctcacg ctcgcggcgc
     3061 cggaagccgg cctcaccctc ggcctcgaag acctcgggga tgcggcgtcc ggcctggcgc
     3121 tcgatctcct cgtcgaggtc gatgaacgcc gccgcgtgtc gcgccgcgac ccgccgcccg
     3181 acggccgtct tcccgctccc gggcagtccg acgacgacga gctccatcga tcagtcgtcc
     3241 ccgcctgatg cggcctcgtc gtccgccgcc ggcgccgact tcggggcggt cgcgccgtcg
     3301 cccgcgggcg cacccgtgag ccgcttgcgc cagcgcgcca tcgactccct gacctcggcc
     3361 atcgagtcgc cgcccgtctt ctcgagcacc gccgccgcca gggtcagcat gaccatcgcc
     3421 tcgccaacga cgcccgcggc gggcacgacg ctgatgtcgc tgcgctcgta gtgcgccttc
     3481 tcgaccgcct cgccggtgcg caggtccgcg gacgggagcg gtcgtgcgag ggtcgagatg
     3541 ggcttgaccg cgccgcgcac gacgatcggc tcgccgttcg acacgcccgc ggtcagaccg
     3601 ccggcgttgt tcgagcgatg gatccagcgg cccgcctcgc cgcggccctc gatcacgtcg
     3661 tggacctgcg acccgaaacg ccgcgtctgc tcgaacccga gcccgagctc gacgcccttg
     3721 acgatgttga tgctcatcac cgccgcggcg agcgccgcat cgagccgtcg atcccagtgg
     3781 acatacgacc cgagcccgat cggcacaccg gtggccacga cctcgaacac gccgccaacc
     3841 gtgtcgccgg cgtcgcgcgc ctcatcgatg cgggcgatca tcgcgacttc tgcgtcgggg
     3901 tccgggcagc gcaggggcga cgcatccgcg tcctcgcgcg agcgggtcgc gttcgcggga
     3961 tccacggcga ccccgccgac ctcggcggtg aacgaccaga cgtcgatgcc gagctccttc
     4021 aggaacgcgc gggccacgcc cccggcggcg acgcgcgccg cggtctcgcg agcggacgcg
     4081 cgctcgagca cgttacgcac gtcgtcgtgg ccgtacttga gcgcgccggc caggtcaccg
     4141 tgaccgggcc gtacccgcgt gaccggggtg gatcgcttgt cgccgccctc cgccgccgcc
     4201 agtagggcag cggcctcctc gtcggtgagc ggctcgacct gcatcacccg agtccagttc
     4261 tcccaatcct tgttcgcgac gagcaggagg atcggcgagc ccagcgtccg gccgtggcga
     4321 accccagcca cgatctcggc gcggtcgtgc tcgatctgct ggcgcgcccc gcggccgtag
     4381 ccgcgctggc ggcgagcgag gtcggaggtg agatccgcct cggccagggc gaggccggcc
     4441 gggacgccct cgatcgtcac gccgagcgtt cgcccgtgcg attcgcccgc ggtcaggaaa
     4501 cggaacggga cggtcatggc gtgtcggtca gctcgatctc agaaaggcca atgcgcgtcg
     4561 cgcgtcagcc ggccggtgcc gcggtttccg gaaccgccgc ggcggcctca ccagcgtccc
     4621 ctgccggctc gccctcggcg gagcggagcc ctgtggcgcg cgcggcgacg agcgcctcgc
     4681 tcatgacctc caacggggcc g
//
LOCUS       DATBSW010000154         7087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_825232, whole genome shotgun sequence.
ACCESSION   DATBSW010000154 DATBSW010000000
VERSION     DATBSW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825232"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VIF63_04250"
     CDS             complement(<1..584)
                     /locus_tag="VIF63_04250"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8238627.1"
                     /translation="MGSAAWFPEQIQGDLVVLRKHVPGNVAAFQRWYADPEVARLARY
                     QDAPMRSDEIDRFFQLRALGSESLTMAIHDRETNRLIGSCAISQVDGDNGSAMYHITI
                     GEKDMWGRGYGTEATQLMIDHAFGTLGLHRVALTVFEFNERAIRAYRRCGFVVEGRSR
                     ESIWRDGRWWDELAMSVLSTEWHERRNPATNAPNS"
     gene            610..2349
                     /locus_tag="VIF63_04255"
     CDS             610..2349
                     /locus_tag="VIF63_04255"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8238628.1"
                     /translation="MRRWSDLAERVAATTRTSEKTGLLADYLRTLSAEELPIAVVFLT
                     GRPFPEADQRATGIGWATIANALALVSGTDRGTLGEAYDRFSDISAAVGDVLANAGHA
                     PDPGGSPTLVEVAATFAAIESASGPARKAKLLADLLARTDPKTARGIVRILSGELRIG
                     LREGLVEAALAKAYDRPLDAVKRAGMLTGDIGATAKLALAGNLEGAALALFHPLKFML
                     ASPAEDAAEIMTRLGPTVWVEDKYDGIRCQLHREGTTVRLYSRDLHDISNQFPEVVES
                     ARDLPWAGILDGELLAWRDGTVLPFIALQGRLGRKDPSATILAEVPVIYVAWDVLGLD
                     EPSSDAAGATADRDGVVAPTLELPLTERRRLLEGLHLPLAEDGGGFALSHLSEAESID
                     ALEAAFAEARARRNEGLMVKDPSSIYSPGRRGYGWLKMKKALATIDCVVVGVEVGHGK
                     RHGVLSDYTFAVRDEARDQLVTIGKAYSGLTDAEIATMTRWFDEHTISHHGRYRAVEP
                     TVVVEVAFDVILRSNRHRSGFALRFPRIARLRLDKDPREADTLESVTRLYEGLQAGAE
                     HLVTAGARKSATS"
     gene            2390..3097
                     /locus_tag="VIF63_04260"
     CDS             2390..3097
                     /locus_tag="VIF63_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8238629.1"
                     /translation="MTTQRFGLELACAAMARSPLFHGIEPDELTRIATAMVRRRYRRG
                     EVIFHEGDPGDSLHVVVEGRVKITRESADGDEAIVVILSPGDSFGELVLLDGAPRSAT
                     ATAMDATETLTMSRATFVELIDGASPFRWQALTGVARRIRRLTDQLAEVHFLDLAGRL
                     ALQLTRLAEEAAPGQTSDVRLSRSLTQSDLAAMVGGTRQRVNQILGDFADEGLVSVDA
                     GGMTIRDLERLRERASW"
     gene            3207..3767
                     /locus_tag="VIF63_04265"
     CDS             3207..3767
                     /locus_tag="VIF63_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238630.1"
                     /translation="MQIPFSAFAVDCTVSGEIALRTDRLSDFLASTVEFEVASPSFRA
                     LDDGRTVTAESCAIERDDLCLILATGPRGQAGRRLWTRQHPVRARVGPYVVIGYMHSP
                     PTIDPFRTTDRRPIVALTSCRVGYVAQGESIWVEAEAVLINTAKMDHLETASPEDLGL
                     AEGLEPDATVDPAAKDMTYGQGLYGD"
     gene            complement(3774..4622)
                     /locus_tag="VIF63_04270"
     CDS             complement(3774..4622)
                     /locus_tag="VIF63_04270"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8238631.1"
                     /translation="MTGFARPELLASTEWLADELSRPDVRVVDVRWRPDGTGEAVFAS
                     GHIPGAAYLDWRSELNDAPSDETGHSLRLAPPDSVAAFMRRAGVSAATTLVLYDDTLG
                     LYAARAWWSLRVYGFESARILDGGFPAWRAESRPVSTGELPHPEGSFTPRLNPRLRLS
                     TTDVRSLLGSPDAQLIDGRANPEYRGHEGNARRLGHIPGAVSLPVGSMHEPGTQRLLD
                     ADAIRAQLTRAAVARGKRLVCYDGSGVGAAKLAFVLTLMGYDDVAVYDGGWAEWGDRL
                     DLPVER"
     gene            4661..5896
                     /locus_tag="VIF63_04275"
     CDS             4661..5896
                     /locus_tag="VIF63_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8238632.1"
                     /translation="MVSPFMSDTDKVTAVRAALPSLSAAIQLNTGSVGPIPAESAAAM
                     AELAAYERDFGRAQFEYWEELKQRIDEARAGVAAVLGGELDEVALTHAATDGMNIGTW
                     AIDWSQGGRAVTTCQEHPGGVGPLYMVGDRYGVDLGFAEFVPDASDDEIVAQFDRLIT
                     PGTKLISLSHVLWTSGLVMPVRRIADIAHARGALVLIDGAQSAGAIPVNVRELGADMY
                     AIPAQKWLLGPEGLGAMWVKRELLDGMRSTFASFSTFASSDSRGNRMLATDARRFQLA
                     GFYRPSVVGMGRSIGWLTMFVGLEFVYRRGAEMAHLAADGLAAIEGVTLLTPRDRMAG
                     LVSFQIAGWEPQAALDELQARTHCIARTLPPVNALRISVGFWTTEDEIATFVDCVRLL
                     AAHTPETLPPRRTLTILNG"
     gene            5893..6270
                     /locus_tag="VIF63_04280"
     CDS             5893..6270
                     /locus_tag="VIF63_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238633.1"
                     /translation="MMSAPARRSWLQVRWRQFRNAPRPVVRAVGSSLAVALILGSAYL
                     AYDLSAPASETGGRTMFGLLYVLGVLLAGSFITWLVVPLPRGSGSRAVRTPWSAMLGL
                     FAAIPIAYLVLVLAFAVVKPLLV"
     gene            6597..6674
                     /locus_tag="VIF63_04285"
     tRNA            6597..6674
                     /locus_tag="VIF63_04285"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6632..6634,aa:Ile,seq:tat)
     gene            complement(6748..>7087)
                     /locus_tag="VIF63_04290"
     CDS             complement(6748..>7087)
                     /locus_tag="VIF63_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238634.1"
                     /translation="EELPPVGAVMSRGVRSWEVRVGNGQIRTIENPLLLAAHDAERVR
                     SWLSPEDSDHVIKVYAAVVGTDPRVERSPSVALISPAQVAEWLSSLPPQKSLDSGRRE
                     KIVKQIRAAL"
BASE COUNT         1018 a         2551 c         2444 g         1074 t
ORIGIN      
        1 gagttcgggg cgttcgtcgc cgggttgcgg cgctcgtgcc actcggtcga gagcacgctc
       61 atcgccagct cgtcccacca tcgcccgtcg cgccagatcg actcgcgtga ccgcccctcg
      121 acgacgaagc cgcagcggcg atacgcccgg atcgcgcgtt cgttgaactc gaagaccgtg
      181 agcgccacgc gatgcagccc gagcgtgccg aaggcgtgat cgatcatcag ctgggtcgcc
      241 tcggtgccgt agccgcggcc ccacatgtcc ttctcgccga tggtgatgtg gtacatcgcg
      301 gagccgttgt cgccgtcgac ctgggagatg gcgcagctgc cgatgagccg gttcgtctcg
      361 cgatcgtgga tggccatcgt gagcgactcg gaaccgaggg cgcgcagctg gaagaagcgg
      421 tcgatctcgt ccgagcgcat cggcgcgtcc tggtagcgag cgagccgggc cacctcgggg
      481 tcggcgtacc accgctggaa cgccgcgacg ttccctggaa cgtgcttgcg gagcaccacc
      541 aggtccccct ggatctgctc cgggaaccac gcagcgctac ccatgaaggc acggtatacc
      601 atcgcccgca tgcggcgctg gagcgacctc gccgagcgcg tcgccgccac gacccgcacc
      661 tccgagaaga ccggcctcct cgccgactat ctccggaccc tgtccgccga agagctgccg
      721 atcgcggtcg tgttcctcac cggccggccc ttccccgagg ccgaccagcg ggcgacgggc
      781 atcggctggg cgaccatcgc gaacgccctt gccctcgtca gcggcacgga ccggggcacc
      841 ctcggcgagg cgtacgaccg gttctccgac atcagcgcgg cggtcggcga cgtcctcgcc
      901 aatgccgggc acgcgccgga tcctgggggc tcgccgacgc tggtggaggt cgccgcgacg
      961 ttcgccgcca tcgaatcggc gtccggcccc gcgcgcaagg cgaagctgct cgctgacctc
     1021 ctcgcgcgca cggatccgaa gacggcgcgg ggcatcgtcc ggatcctgtc gggcgagctc
     1081 cgcatcgggc tgcgcgaggg cctcgtcgaa gcggcgctcg ccaaggcgta cgaccggccg
     1141 ctcgatgcgg tgaagcgcgc cgggatgctc accggcgaca tcggggcaac ggcgaagctc
     1201 gcgctcgcgg gcaacctgga aggagccgcg ctcgccctct tccacccgct caagttcatg
     1261 ctcgcctcgc cggccgagga cgcggccgag atcatgaccc gcctcggccc gacggtctgg
     1321 gtcgaggaca agtacgacgg cattcgctgt cagctgcatc gcgagggcac gaccgtccgc
     1381 ttgtactccc gcgacctgca cgacatctcg aaccaattcc cggaggtggt cgagtcggcg
     1441 cgggacctgc cctgggctgg aatcctcgac ggcgagctcc tcgcctggcg cgacggcacg
     1501 gtgctgccgt tcatcgcctt gcagggacgc ctcgggcgca aggatccgtc cgcgacgatt
     1561 ctcgccgagg tgccggtcat ctacgtggcc tgggatgtgc tgggcctgga cgagccgtcc
     1621 tcggacgccg ccggcgccac ggccgaccgg gacggcgtcg tggcgccgac cctcgagctg
     1681 cccctcaccg agcgccgtcg tttgctcgaa ggcctgcact tgccgctcgc cgaggacggc
     1741 ggcggattcg ccctgtcgca cctgtccgag gcggagtcga tcgacgccct cgaggccgcg
     1801 ttcgccgagg cccgcgcccg gcgcaacgag ggcctcatgg tcaaggaccc gagctcgatc
     1861 tactcaccgg gccggcgcgg ctacggctgg ctgaagatga agaaggcgct cgcgacgatc
     1921 gactgcgtgg tcgtcggcgt cgaggtcggc cacggcaaac ggcacggcgt gctctcggac
     1981 tacacgtttg ccgtgcgcga cgaggcgcgc gatcagctgg tcacgatcgg caaggcctac
     2041 agcggcctca ccgacgccga gatcgcgacg atgacccgct ggttcgacga gcacacgatc
     2101 agccaccacg ggcggtaccg tgcggtcgag ccgactgtcg tcgtcgaggt cgcgttcgac
     2161 gtcatcctgc gctcgaaccg gcacaggtcc ggcttcgccc tgcgcttccc gcggatcgcc
     2221 cggctgcgcc tcgacaagga cccccgcgaa gccgacacgc tcgagtccgt gacccgcctg
     2281 tacgagggcc tccaggccgg cgccgagcac ctcgtcaccg ccggcgcgcg caagtcggcg
     2341 acgtcctgat ttcgcacgcc cgcaggcgct cgcgtacgct ccgcgagcct tgaccaccca
     2401 acggttcgga ctcgagctcg cgtgtgcagc catggcgcgc tcgcccctgt tccacggcat
     2461 cgagcccgac gagctgaccc ggatcgcaac cgccatggtc cgacgccgct atcggcgcgg
     2521 cgaggtcatc ttccacgaag gtgatccggg cgactcgctc catgtcgtcg tcgagggccg
     2581 ggtcaagatc acccgtgagt cggccgatgg cgacgaggcg atcgtcgtga tcctctcgcc
     2641 cggcgattcc ttcggcgagc tcgtgctgct cgacggcgcg ccgcgatcgg caacggcgac
     2701 cgcgatggat gcgaccgaga ccttgacgat gtcgcgcgcc accttcgtcg agctcatcga
     2761 tggtgccagc ccgttccgat ggcaggccct caccggcgtg gcccgtcgca taaggcgcct
     2821 gacggaccag ctggccgagg tccacttcct cgacctcgcc ggccgcctcg ccctccagct
     2881 gacgcgcctc gccgaagagg cggcaccggg ccagacctcg gacgtccgcc tgagccggtc
     2941 gctcacccag tccgacctgg cggcgatggt cggcgggaca cggcagcgcg tgaaccagat
     3001 cctcggcgac ttcgccgacg agggcctcgt ctcggtcgac gccgggggca tgacgatccg
     3061 cgacctcgag cgcctccggg agcgcgccag ctggtagcag ccggtacctg aatccccgcg
     3121 ccggagcggt tgcactcctg tagattcgca gcacggccgg atgcctgtca tcgggcctgc
     3181 aaggtcgcca gaacagattg cacgcgatgc agattccctt cagtgcattt gcagtggact
     3241 gcacggtctc gggcgagatc gcgctccgga ctgaccggct gtccgacttc ctggcgtcga
     3301 ccgtcgagtt cgaggtcgcg agcccctcat tccgcgccct cgacgacggt cggacggtca
     3361 cggcggagtc ctgcgcaatc gagcgcgacg atctctgcct gatcctggca accgggccgc
     3421 gaggtcaggc cgggcgacgg ctctggaccc ggcagcaccc ggtccgggct cgcgtcgggc
     3481 cgtacgtcgt catcggctac atgcactcac ccccgacgat cgatccgttc cggacaaccg
     3541 accgccgccc gatcgtcgcg ctgacgtcgt gccgcgtcgg ctacgtcgcc cagggcgaga
     3601 gcatctgggt ggaggccgag gccgtcctca tcaacacggc gaagatggac cacctcgaga
     3661 ccgcgagccc ggaggacctc ggcctcgccg agggcctcga gcccgacgcg accgtcgacc
     3721 ccgccgccaa ggacatgacc tacggccagg ggctgtacgg cgactagccg gccctagcgc
     3781 tccaccggca ggtcgaggcg atcgccccac tcggcccagc caccgtcgta gaccgcgacg
     3841 tcgtcgtagc ccatgagcgt gagcacgaag gccagcttcg ccgcgcccac gcccgacccg
     3901 tcgtagcaga ccaggcgctt gccgcgggcg acggcggcgc gcgtcagctg ggcgcggatg
     3961 gcgtcggcgt cgaggaggcg ctgggtccca ggctcgtgca ttgacccaac cggcaggctc
     4021 accgcgcccg gaatgtggcc gaggcgccgg gcattgccct cgtggccgcg gtactcgggg
     4081 ttcgcccggc catcgatcag ctgggcgtcg ggcgagccga gtagcgagcg cacgtccgtg
     4141 gtggacagtc gcagcctcgg gttcagccgc ggcgtaaacg agccctccgg gtggggcagc
     4201 tcacccgtgc tcaccggtcg tgattcggcc cgccaggccg ggaacccgcc atcgaggatt
     4261 cgcgcggatt cgaacccgta gacgcgcagc gaccaccagg cgcgcgccgc gtacagcccg
     4321 agcgtgtcgt cgtacaggac gagcgtcgtc gcagcgctga cgcccgcacg ccgcatgaac
     4381 gccgcaaccg agtccggcgg ggcgaggcgc agcgagtggc ccgtctcgtc cgacggcgcg
     4441 tcgttgagct cggagcgcca gtcgaggtag gcggcgccgg ggatgtgccc ggacgcgaac
     4501 accgcctcgc ctgtgccgtc cggccgccag cgcacgtcga cgacccgcac gtccggccgg
     4561 ctcagctcgt cggccagcca ttcggtcgag gcgagcagct cggggcgcgc gaacccggtc
     4621 acgagtcgat cgggaagtac atcgcccgta tcataggtgc gtggtttccc cgttcatgtc
     4681 cgacaccgac aaggtcaccg cggtgcgcgc cgcgctgccg tcactctcgg ccgcgatcca
     4741 gctgaacact gggtcggtcg ggccgatccc cgccgaatct gcggcagcga tggccgagct
     4801 ggccgcgtac gagcgcgact tcggccgggc ccagttcgag tactgggagg agctgaagca
     4861 gcggatcgac gaggcccgcg ccggtgtggc ggcggtgctc ggcggcgagc tcgacgaggt
     4921 ggccctcacg cacgcagcca ccgacggcat gaacatcggc acctgggcaa tcgactggag
     4981 ccagggtggc cgcgccgtca ccacgtgcca ggagcatccc ggtggcgtcg gcccgctgta
     5041 catggtcggc gatcggtacg gcgtcgacct cggattcgcc gagttcgtcc cggacgcttc
     5101 ggacgacgag atcgttgccc agttcgaccg cctcatcacc ccgggcacga agctgatctc
     5161 gctctcgcac gtgctctgga cgagcgggct cgtcatgccc gtccggcgga tcgccgacat
     5221 cgcccacgct cgcggcgccc tggtcctgat tgacggtgcc cagtccgctg gagcgatacc
     5281 ggtgaatgtg cgcgagctcg gcgccgacat gtacgccatc ccggcccaga agtggctcct
     5341 cggtcccgag ggtctcgggg cgatgtgggt gaagcgcgag ctgctcgatg gcatgcgctc
     5401 gacgttcgcg agcttctcga ccttcgccag ctcggactcg cgcggcaacc ggatgctcgc
     5461 gaccgatgcc cggcggttcc agctggccgg cttctatcgg ccgtcggttg tcggcatggg
     5521 tcgctcgatc ggctggctga cgatgttcgt cgggctcgag ttcgtgtacc gccgcggcgc
     5581 cgagatggcg cacctcgcgg cggacggcct cgccgcgatc gagggcgtga cgctgctgac
     5641 gccgcgggat cgcatggccg ggctcgtgtc gttccagatc gcgggctggg agccgcaggc
     5701 ggcgctcgac gagctccagg cgcgcacgca ctgcatcgcg cgaaccctgc cgccggtcaa
     5761 cgcgttgcgg atcagcgtcg gcttctggac gaccgaggac gagatcgcga ccttcgtcga
     5821 ctgcgtccgg ctgcttgccg cgcacacccc cgagacgctc ccgccacgac gcacgctgac
     5881 gatcctgaac ggatgatgtc ggcgccggcg cggcgctcgt ggctccaggt ccgctggcgc
     5941 cagttccgga acgcaccgcg accggtggtc cgagcggtcg gctccagcct tgcggtcgcc
     6001 ctgatcctcg ggagcgcgta cctcgcctac gacctttcgg ctcccgcctc ggagacgggt
     6061 gggcggacga tgttcggcct cctgtacgtg ctcggcgtgt tgctcgccgg ttccttcatc
     6121 acctggcttg tcgtgccgct cccgcgcggc tcgggctcgc gagcggtcag gacgccgtgg
     6181 agcgcgatgc tggggctgtt cgccgcgatc ccgatcgcgt atctcgtcct cgtcctcgcc
     6241 ttcgcggtcg tcaagccact gctggtctga tcgccgacgc gcgagccact ccattcctcc
     6301 gctgcggatt ccaatgccca ttggcacata agcgactgcg tctggggaag gcggacagcc
     6361 aggagccaac gatggccggt gatgccgggc ttctgacgga ccgtgttgtc gcggcccgcg
     6421 gtgtctaggc ccgcgttgtg tccgttatgc agcaattggc atagatttcc ggcgccccgc
     6481 gagcgagggc cgtcgccgag caagcagatg aacgcttggg tagacgcacc ggcttgcagg
     6541 tacttcaacc tcgcacgccg agcgctgcac gcgagtaccc ggcataagat tgctgcgggg
     6601 gagtagccca gtcggttaga ggcgccgagc ttatacctcg gaagtcggag gttcgagtcc
     6661 tccctcccct accaccagcg gcagggtcgt ccgcgccggg acgcgcccgg acgcgccggg
     6721 tgcggcgcga cagcgccgag cccgcggcta cagcgccgcg cggatctgct tgacgatctt
     6781 ttcgcgacgc ccgctgtcga gcgacttctg cggcggcagt gacgacagcc attccgcgac
     6841 ctgcgcgggg ctgatgaggg cgaccgacgg cgagcgctcg acccgcgggt cggtgccgac
     6901 aaccgcggcg tacaccttga tcacgtggtc gctgtcttcc ggcgatagcc agctgcggac
     6961 gcgctcggcg tcgtgggccg cgaggagcag cggattctcg atcgtgcgga tctggccgtt
     7021 gccgactcgg acctcccagc tgcggacgcc gcgggacatg accgcgccga cgggcgggag
     7081 ctcttcg
//
LOCUS       DATBSW010000155         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_472221, whole genome shotgun sequence.
ACCESSION   DATBSW010000155 DATBSW010000000
VERSION     DATBSW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472221"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VIF63_04295"
     CDS             complement(<1..390)
                     /locus_tag="VIF63_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238635.1"
                     /translation="MSGSNANGPLVLAIESSCDETGIALVQGGRQILSNVVASQVALH
                     APTGGIVPEVAARAHLRWILPVLDEAWQGAGASWDDVGAVAVTRGPGLAGSLLVGINV
                     AKTLAWVHDKPFVGVNHLEGHVYAAWLR"
     gene            complement(387..974)
                     /gene="rimI"
                     /locus_tag="VIF63_04300"
     CDS             complement(387..974)
                     /gene="rimI"
                     /locus_tag="VIF63_04300"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY8238636.1"
                     /translation="MVARPPVALRIESMSFDDIPAVHRIERASFSVPWPDDAYRNELA
                     TNRMASYVVARTDDAIVGFAGLWVMVDEAHVTTFAVDPIWRRRGIGERLLLALLDIAV
                     ARRAREATLEVRLSNMPARRLYEKYGFRPVGIRPRYYSDNGEDALIMTTDALDSVGMR
                     ERVRDLRAALEQRPVEPEPDLDTPAGEPSAEDYRG"
     gene            complement(953..1618)
                     /gene="tsaB"
                     /locus_tag="VIF63_04305"
     CDS             complement(953..1618)
                     /gene="tsaB"
                     /locus_tag="VIF63_04305"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8238637.1"
                     /translation="MTAWLLALDTSTTTIVVAAGAPDGALLDAESFEGRYRHSQELLP
                     AIVRLVERAQLRLPELAGVVVGTGPGAFTGLRVGLATAKTLAHELGIPVVGIPSSSAL
                     LAAVDGAEVLWLPSGPRDRIAVVAGSDPVVVVADATSPAPAGGVELAVDLDGRAPDVA
                     LELGRAALAGLPAALLRLGAARLAAGEADDPERLVPRYASQPRGAPAPDAEGGVAWSR
                     DPR"
     gene            complement(1615..2127)
                     /gene="tsaE"
                     /locus_tag="VIF63_04310"
     CDS             complement(1615..2127)
                     /gene="tsaE"
                     /locus_tag="VIF63_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY8238638.1"
                     /translation="MTTEIEPALEIVSGSPEATRAFAASLASVATPGDIVCLWGELGA
                     GKTVFAKGFGAGLGVRDTILSPSFVLMGEYAGRLPLFHIDLYRLASATEALDGGLLDD
                     RQATGVVLIEWPDRLGASLPADRLDVAIDGGADEPRVLTITAHGTGHARYAAAARAAG
                     AGATRATVAR"
BASE COUNT          339 a          845 c          838 g          277 t
ORIGIN      
        1 acgcagccac gctgcgtaca cgtggccctc gaggtggttc acgcccacga acggcttgtc
       61 gtggacccac gccagcgtct tcgcgacgtt gatcccgacc aggagcgacc cggcgagacc
      121 cggcccgcga gtgaccgcga ccgcgccgac gtcgtcccac gacgcgccgg cgccctgcca
      181 cgcctcgtcc agcacgggca ggatccagcg caggtgggca cgggccgcga cctcgggcac
      241 gatcccgccc gtcggggcat gcagcgcaac ctggctggca acgacgttcg acaggatctg
      301 gcgtccaccc tgcacgagcg cgatcccggt ctcgtcgcac gacgactcga tcgcgaggac
      361 gagcgggcca ttcgcgttcg aaccgctcat ccgcggtagt cctcggccga cggctcaccc
      421 gccggcgtat cgagatcggg ctcgggctcg accgggcgct gctcgagcgc cgctcgcagg
      481 tcgcgaacgc gctcgcgcat ccccacagaa tcgagggcat cggtggtcat gatcagtgcg
      541 tcctcgccgt tgtccgagta gtagcgcggg cgaatgccga ccggccggaa cccgtacttc
      601 tcgtacaggc gccgggcggg catgttcgag aggcgcacct cgagggtggc ctcgcgggcg
      661 cggcgggcaa ccgcgatgtc gaggagcgcg aggaggagcc gctcgccaat cccccggcgg
      721 cgccagatcg gatcgaccgc gaacgtggtg acgtgggctt cgtcgaccat cacccacagc
      781 ccggcgaagc cgacgatcgc gtcgtcggtc cgggccacga catagctggc catgcggttc
      841 gtcgcgagct cgttgcggta ggcatcgtcc ggccagggca cggagaagct ggcccgctcg
      901 atccggtgga ctgccgggat gtcgtcgaac gacatcgact cgatccggag cgctaccggg
      961 ggtcgcgcga ccacgccact cccccttctg cgtccggcgc cggtgcgcca cgcggttggc
     1021 tcgcgtaccg gggcacgagc cgctcgggat cgtctgcctc gccggcggcc agtcgagccg
     1081 ccccgagccg gaggagcgcc gccggcaggc ccgcgagcgc agccctgccg agctcgaggg
     1141 ccacgtccgg cgcccggcca tcgaggtcga cggcgagctc gacgccgccg gcaggcgccg
     1201 gcgaggtcgc atcggcgacg acgaccacag ggtcgctgcc cgccacgacc gcgattcgat
     1261 cacgaggccc ggagggcagc cagagcacct cggcgccatc cacggccgcg agcagcgccg
     1321 aggacgatgg gatcccgacg accgggatgc ccagctcgtg cgcgagggtc ttcgcggtgg
     1381 cgagcccgac gcgcaatccg gtgaatgctc cgggacccgt gcccacgacg acgccggcaa
     1441 gctcggggag tcggagctgc gcgcgctcca cgaggcgcac gatcgccgga aggagctcct
     1501 gggagtggcg gtaccggcct tcgaacgatt cggcgtccag gagcgcgccg tcgggggccc
     1561 cggcggccac gacgatcgtt gtcgtcgagg tgtcgagcgc gagcagccag gcggtcatcg
     1621 cgcgaccgtg gcgcgagtgg cgcccgctcc cgcggcgcgc gcggccgcgg cgtagcgcgc
     1681 gtggcccgtt ccatgcgcgg tgatggtcag gacgcgtggc tcatcggccc cgccgtcgat
     1741 cgccacgtcc agccggtcgg cgggcaacga tgccccgagg cgatccggcc actcgatgag
     1801 cacgacgccg gtcgcctggc gatcgtcgag gagcccgccg tcgagcgcct cggttgccga
     1861 cgcgaggcgg tacaggtcga tgtgaaacag cggcagccgg cccgcgtact cgcccatgag
     1921 cacgaagctc ggcgagagga tcgtgtcgcg cacgccgagg ccggcgccga agcccttggc
     1981 gaacacggtc ttgccggcgc cgagctcgcc ccacaggcac acgatgtcgc ccggggtcgc
     2041 cacggaagcg agactcgccg cgaacgcgcg ggtggcctcg gggctgccgc tcacgatctc
     2101 cagcgccggt tcgatctcgg tcgtcacccg aggctcaggc aaatcgttcg agatcgccga
     2161 tcgggatcgc caccggcggg cggccgccga agcggacgaa cgccgcctcg agcgtgacac
     2221 cggcggggaa tcggcgcggg cccgccagcc cggcccacgt tccctcggcg ccggcgagcg
     2281 gcccggagcg aacccggac
//
LOCUS       DATBSW010000156         4527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_366803, whole genome shotgun sequence.
ACCESSION   DATBSW010000156 DATBSW010000000
VERSION     DATBSW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366803"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VIF63_04315"
     CDS             complement(<1..955)
                     /locus_tag="VIF63_04315"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8238639.1"
                     /translation="MATGWRGPDASQFLPTPRLDDLIAALHAGGRRVVGPVVEDGALK
                     MIEIQHAAELPFGWTARSAPGLVRLERHVDGDPLASRAFDNGPAWSGIKPWTFPSRVD
                     ALSVETREDGSLAVKVEAPTPQPTAIIGARACDLAALAVHDRVLVGGPAVDPDYAARR
                     ADLLVIAVECALATSTCFCTSMGTGPEVTTGADVVLAEVDGGFVARSGSPAGERLLAG
                     LGLATADVALATQATEQVAAVRAGMGHAVEAEGLHDRLLANLDSPRWAEIAERCLACA
                     NCTLVCPTCFCTGTSITSDLDGRESSAARQWDSCFTAGFAQV"
     gene            complement(955..1416)
                     /locus_tag="VIF63_04320"
     CDS             complement(955..1416)
                     /locus_tag="VIF63_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8238640.1"
                     /translation="MATRTETAFDHLSPAAHAHLVELARPIEYEAGAVVLREGVDTPF
                     LGALLEGRVALRLRIPERGERLTIVTVEPGQLLGWSAVVPPYRATVDAFATEPTRVLA
                     IDAAALRERLAGDCELAAELLPLVLESVSMRLSASWQQLLDVFETGGVEPW"
     gene            complement(1594..2865)
                     /locus_tag="VIF63_04325"
     CDS             complement(1594..2865)
                     /locus_tag="VIF63_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8238641.1"
                     /translation="MRIVTPAEAVANIRSGDQLYLHCAAATPSVLLDALVTRAPELAD
                     VSVVHLHCEGPGPHLAPEMAGHFRHRALFVGPNARAAVQEGRADYVPVFLSDVPRLFT
                     SGMLPLDHVFVNASTPDANGFCSLGTSIEAMHAAIRAGKQVIVQLNRAVPRTLGDSFI
                     HVDDIDLAVECSVPPYEHRLGEIGDVERRIGEFTADLVPDGATLQMGIGVIPAAVGAA
                     LMGKHDLGVHTEMFTDTVVDLVEAGVINGSRKERNRGKIVAAFMMGSQRLYEFANDNP
                     MVEMRPVDFTNDTSVIRTFSRMTAINSAIEIDLTGQIVADSIGHRMYSGVGGQMDFVR
                     GASLAAEGQAIIALPATAMNGARSRIVASLQEGAGVVTTRAHARTVVTEYGIAELWGR
                     SLRERAKALIAIADPAFRDELEHDARRLHLA"
     gene            complement(2868..3680)
                     /locus_tag="VIF63_04330"
     CDS             complement(2868..3680)
                     /locus_tag="VIF63_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238642.1"
                     /translation="MSALRVATPACRGLAPGATRTAAEHRDLIGHLDDTATWPGAAAS
                     LLARLGFRLVGPSSLGGGGSHLLVALRDRPTLDHFDPEALAYFASDGDRAALTTLDRR
                     AVLADNGAASRRVLWGHVHVVDRLPVENRFLTFGGELRVAPVDSKLTVLDLHSPAPIV
                     RWGGHSQVTDTLTDAIGAFFARLMVPIDFVPGAESRIDALAPEVLYHAFLLDWRARAA
                     RAERHGAEPGPLAGWAGAACRRACIDAEARRSAEALLAELPGGSSARQGPIV"
     gene            3868..4221
                     /locus_tag="VIF63_04335"
     CDS             3868..4221
                     /locus_tag="VIF63_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238643.1"
                     /translation="MIATTATQPTPAASAPQPDAVVWVDEQHAIVARADDGGIATTEV
                     RRFGQPETRFLANVVHEIIGREHVMIVGPQPIRIALERAFVAISHRPDRLIAVPRTAN
                     AAGAEILDRLERLAA"
     gene            complement(4293..>4527)
                     /locus_tag="VIF63_04340"
     CDS             complement(4293..>4527)
                     /locus_tag="VIF63_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238644.1"
                     /translation="PAFLTDAALDVRARGTRVFFGRWDPSDPPIGVGERLFRILPVSK
                     ELMPIGDFRDWPAIEAWAREIARELAREAVAAT"
BASE COUNT          687 a         1589 c         1640 g          611 t
ORIGIN      
        1 cgacctgcgc gaagccggcg gtgaagcagc tgtcccattg ccgcgcggcg gaggattcgc
       61 ggccgtcgag atccgaggtg atcgacgtgc ccgtgcagaa gcacgtcggg caaacgaggg
      121 tgcagttcgc gcacgcgaga cagcgctcgg cgatctccgc ccagcggggg ctgtcgaggt
      181 tcgccaggag ccggtcgtgg aggccctcgg cctcgacggc atggcccatt ccggcgcgga
      241 cggccgcgac ctgctcggtg gcctgcgtcg cgagcgcgac gtctgccgtc gcgagcccaa
      301 ggcccgcgag cagccgctcc ccggcggggc tcccggatcg ggcgacgaag cctccgtcga
      361 cctcggcgag gaccacgtcg gcgccggtgg tcacctcggg cccggtgccc atcgaggtgc
      421 agaagcaggt ggaggtggcg agcgcgcact cgacggcgat gacgagcagg tcggcgcgac
      481 gcgcggcgta gtcgggatcg acggccgggc cgccgacgag cacgcggtcg tggacggcga
      541 gcgcggcgag gtcgcaggca cgagcgccga tgatcgcggt cggctggggg gttggtgcct
      601 cgaccttgac ggccagcgag ccgtcctcgc gggtctcgac cgacagggca tcgacccggg
      661 acgggaacgt ccacggcttg atgccgctcc acgccgggcc gttgtcgaac gcgcggctgg
      721 caagcgggtc accgtccacg tggcgctcga ggcggacgag acccggtgcg ctccgcgcgg
      781 tccacccgaa cggcagctcg gcagcgtgct ggatctcgat catcttgagc gcgccgtcct
      841 cgacgaccgg gccgaccacc ctgcggccgc ccgcgtggag cgctgcaatc aggtcgtcca
      901 gtcgcggcgt gggcaggaat tggctcgcgt ccgggcctcg ccatccagtc gccatcacca
      961 cggctcgacc cccccggtct cgaacacgtc gaggagctgc tgccagctcg cgctgaggcg
     1021 catcgagacg gattccagga ccagcggcag gagctcggcc gccagctcgc agtcgcccgc
     1081 gaggcgctcg cggagcgccg cggcgtcgat ggccaggacg cgggtgggct ccgtcgcgaa
     1141 cgcgtcgacc gtcgcacggt acggtggaac cacggcagac cagccgagca gctggccggg
     1201 ttcgacggtc acgatggtga ggcgctcacc gcgctcgggg attcggagcc gaagcgcgac
     1261 gcgaccttcg agcaaggcgc caaggaatgg cgtgtccaca ccctcgcgca gcacgacagc
     1321 gccggcttcg tactcgatcg gccgcgcgag ctcgacaagg tgggcgtgcg cagccggtga
     1381 gaggtgatca aaggcggtct cggttcgcgt ggccatgcat acctccgtgc agccaccgta
     1441 ccgacggcga accgcgcccg gcatagccaa ccggccccat cgcgccgggc cgaggtcccg
     1501 ccctggacgt gacctacggc ctcccgacgc cgcaggtacg ggccgttcgg cccggtgtgc
     1561 gcgcaggttc tcgagcgatc gaccggcgac gggtcaggcc aggtggagcc gccgtgcgtc
     1621 gtgctcgagc tcgtcgcgaa acgcgggatc cgcgatggcg atgagcgcct tggctcgctc
     1681 gcggagggag cggccccaca gctcggcaat cccgtattcg gtgacgaccg tccgggcatg
     1741 tgccctcgtc gtcacgacgc cggcgccctc ctgcagcgat gccacgatcc gcgaccgcgc
     1801 cccgttcatg gcggtcgccg gcagggcgat gatcgcctgg ccctcggctg cgagcgacgc
     1861 gccccggacg aagtccatct ggccacccac gcccgagtac atccggtggc cgatcgagtc
     1921 cgccacgatc tggcccgtca ggtcgatctc gatcgcggag ttgatcgccg tcatccggct
     1981 gaacgtgcgg atgacggatg tgtcgttcgt gaagtccacg gggcgcatct cgaccatcgg
     2041 gttgtcgttg gcgaactcgt agagccgctg cgagcccatc atgaacgcgg cgacgatctt
     2101 gccgcggttc cgctccttgc gcgagccgtt gatcacgccg gcctcgacga ggtcgaccac
     2161 cgtgtccgtg aacatctccg tatggacgcc gaggtcgtgc ttgcccatca gggcggcacc
     2221 gaccgccgcc gggatcacgc cgatgcccat ctgcagcgtg gcgccatccg gtaccaggtc
     2281 cgccgtgaac tcgccgatcc gtcgttccac gtcgccgatc tcgccgagcc ggtgctcgta
     2341 gggcggcacc gagcactcga cggccaggtc gatgtcatcg acatggatga agctgtcgcc
     2401 cagcgtcctc gggacggcgc ggttcaactg cacgatcacc tgcttcccgg cccggatcgc
     2461 ggcgtgcatc gcctcgatgc tcgtccccag cgagcagaag ccgttggcat cgggcgtgct
     2521 cgcgttcacg aacacgtgat cgagcgggag catcccggag gtgaagagcc gagggacatc
     2581 ggacaggaac accggcacgt agtcggcgcg accttcctgg acggcggcgc gagcgttcgg
     2641 gccgacgaac agcgcccggt gccggaagtg accggccatc tccggcgcga gatgtggccc
     2701 cggaccctcg cagtgcaggt ggacgacgct gacatccgca agctctggcg cccgggtgac
     2761 cagggcatcc agcaggaccg atggggtcgc ggcagcgcag tggaggtaga gctgatcgcc
     2821 ggatcggatg ttggcgacgg cctcggcagg cgtaacgatt cgcatggcta gacgataggg
     2881 ccctgccgcg ccgacgaccc gcccggcagc tcggccagga gggcttcggc actgcgacgc
     2941 gcctcggcgt cgatgcacgc gcgacggcat gccgcgccgg cccaaccggc gagcggcccg
     3001 ggctcggcgc cgtgccgttc cgcgcgggcg gcgcgggccc gccagtccag gaggaacgcg
     3061 tggtacagca cctccggggc gagggcatcg atccgcgact cggcgcccgg cacgaagtcg
     3121 atgggcacca tcaggcgggc gaagaacgct ccgatcgcat cggtcagtgt gtcggtcacc
     3181 tggctgtggc caccccagcg gacgatcggc gccggggagt ggaggtcgag gacggtgagc
     3241 ttcgagtcga ccggcgcgac gcggagctcg ccaccaaagg tgaggaaccg gttctcgacc
     3301 ggcaggcgat cgaccacgtg cacgtggccc cacagcaccc gtcgcgaagc cgcgccgttg
     3361 tccgcgagga cggctcttcg atcgagcgtg gtcagcgcgg cccggtcgcc gtcgctggcg
     3421 aagtaggcga gcgcttcagg atcgaagtgg tcgagcgtcg gcctgtcgcg gagggccacg
     3481 aggagatggc tcccaccgcc gccgagcgag ctcggaccga cgagccggaa gccgaggcgg
     3541 gcgagcaggg acgccgcggc gccgggccac gtcgccgtgt cgtcgaggtg gccgatcagg
     3601 tcgcggtgct cggccgcggt gcgcgtcgcg cccggcgcga ggccgcggca cgcaggcgtc
     3661 gcgactcgaa gggcactcac gggacgagca tggctcgcga acggtgatcc gggcagggcc
     3721 gttcagcccg ccgcagggct gcgaacgtcc cgtccatggg ggtccggagg ttcgtcgatc
     3781 gccgccaatc ggcacccaag ggcaaggccg aacggccgtt cctcgatgat cgcgagccaa
     3841 ctaccttcga tgacaggagg tgcagccatg atcgccacca ccgcaacaca gccaacaccc
     3901 gccgcttcgg cgccccagcc ggacgccgtc gtctgggtgg acgagcagca cgctatcgtc
     3961 gcccgcgcgg acgacggagg tatcgcgacc accgaggtcc ggcgcttcgg ccagcccgag
     4021 acgcgcttcc tggcgaacgt tgtccacgag atcattggac gcgagcacgt gatgatcgtc
     4081 gggccgcagc ccattcgcat cgcgcttgag cgtgcgttcg tcgccatcag ccatcgacct
     4141 gaccggctca tcgccgttcc gcgaacggcg aacgccgccg gcgcggagat cctcgaccgt
     4201 ctcgagcggc tcgccgcctg accgcgctcg tgacctgact gccctcctgg ccggccggcc
     4261 gcggccgtac ggtccggcgg ccggcctgtc gattacgtgg ccgctaccgc ctcccgtgcg
     4321 agctctcgag cgatttcccg agcccaggcc tcgatcgcgg gccagtcgcg gaagtcgccg
     4381 atcggcatca gctccttcga aaccggcagg attcggaaga ggcgctcgcc gacgccgatg
     4441 gggggatccg aggggtccca tcgcccgaag aacacccggg tgccgcgcgc cctgacgtcg
     4501 agtgccgcgt cggtcaggaa ggcaggc
//
LOCUS       DATBSW010000157         5826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1142058, whole genome shotgun sequence.
ACCESSION   DATBSW010000157 DATBSW010000000
VERSION     DATBSW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142058"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VIF63_04345"
     CDS             complement(<1..1477)
                     /locus_tag="VIF63_04345"
                     /inference="COORDINATES: protein motif:HMM:NF040318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238645.1"
                     /translation="MAPEPQPERRESTEPHEATDDLDVLLAALPPEIVAAIHALTDRE
                     ALIEVVMDLGRRPEARYPDSEVTLLDREIVEADLQHVVDHIGVFGDDNRAGIERTLHR
                     ISAIRNRTGKVVGLTLRVGRAVFGTIEIIADFIETGKSLLIMGRPGIGKTTMLREAAR
                     VLADDLDKRVVVVDTSNEIAGDGDIPHPAIGKARRMQVRTPSLQHEVMIEAVENHMPQ
                     VIVIDEIGTELEAQAARTIAERGVQLIGTAHGNTLDNLMLNPTLSDLIGGIQSVTLGD
                     EEARRRRSQKSVLERKAPPTFDVVIEIQDRERVLVHGDVAETIDSILRGDVQSPELRW
                     RDAEGVHRSQSRPRPSPREQLAPDRFSGLIGQGSSWRTEPSWRGDGGFRGDFRGDYRA
                     DPGGRGGSGSGFFPGERGSRPGFRPGASGSWRPGRSPSGSLTAAAGAGAGAPRVEAPF
                     VAGEIADRGPVERGTTPAPEPRAREARELSRQREWRESATKA"
     gene            complement(1467..2552)
                     /locus_tag="VIF63_04350"
     CDS             complement(1467..2552)
                     /locus_tag="VIF63_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238646.1"
                     /translation="MTVADGRPGRPSGKVRAARLTDLAALGELSRIAQTEGSGSRTLG
                     LPVNGPPIGVFSLFRLPLVAFRPHDLLFVYESDRHVSGLVRVERENWRDDWTIVELDA
                     IGGAQPGEIRHRLVQQVLREAQKRGANRFHVACADADDNVELFMQAGFMRYGEERVLY
                     RPPDQPLPRPWTDEHATKARIRQARPVDANALMQLYSAVTPAPVQRLEALRIPDWERQ
                     GSNWRVPRSSLAPILRFADLDGFVQDSPDGARIDAFIQTGLAREDQPHYLRPLGRPDI
                     DLKPLIEFGLGTIAGQVEKNGVSRPNHGVLAPVRTYESPANRRLEESGFAEIATVTLL
                     MRETLVRVAEPSLVPAAVRHLEVTRGS"
     gene            complement(2549..3187)
                     /gene="pdxT"
                     /locus_tag="VIF63_04355"
     CDS             complement(2549..3187)
                     /gene="pdxT"
                     /locus_tag="VIF63_04355"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEY8238647.1"
                     /translation="MKIGVLALQGDFAEHIRMLDAIGVESVEVRLASDLEGISGLILP
                     GGESTTMRRLIERWALREPLLDFAATGAPLFGTCAGMIVLSREIADGDEPILPLLDVT
                     VRRNAFGRQLDSFEGELSVPIVGDQPVHAVFIRAPVIERVGPGVDVLARLDDGRIVAV
                     REKNILATAFHPELADEPRFHRLIATMAAEHDDPGEGSGRRHHPTRRAGLTR"
     gene            complement(3327..4214)
                     /gene="pdxS"
                     /locus_tag="VIF63_04360"
     CDS             complement(3327..4214)
                     /gene="pdxS"
                     /locus_tag="VIF63_04360"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY8238648.1"
                     /translation="MSPETSTWTTKKGLAQMLKGGVIMDVVTAEHAKIAEDAGAVAVM
                     ALERVPADIRAAGGVARMSDPAMIQGIMDAVTIPVMAKARIGHFVEAQILEALGVDYI
                     DESEVLTPADEANHIDKHAFKVPFVCGCRNLGEALRRINEGAAMIRTKGEAGTGNVVQ
                     AVMQLRSVLSEIRRLQSMRDDELFVAAKELQAPFELVKDVAANGRLPVVNFVAGGIST
                     PADAALVMQLGAEGVFVGSGIFKSEDPARMANAIVQATTHFDDPKILADVSKGLGAPM
                     RGIAMEAIPEGERLAVRGW"
     gene            complement(4343..5110)
                     /locus_tag="VIF63_04365"
     CDS             complement(4343..5110)
                     /locus_tag="VIF63_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238649.1"
                     /translation="MTTASSAAQLAAPPSARDDANPASQSAEAIRIRLADAWGEMGAA
                     WGVAPAIARVQAYLMTRQEPLTEREVREALGLSHRAASLALNEAVSWGLVERVTEPRR
                     VGRRGPAGVAYQAVGDHWRWFGRVVAERKTREGDPIISLLERSGGEAADELRRHPGDP
                     ELVALHEWLSTFLVFVRLFDRAVGLVPRLEPRQLERALRLLGEVDDDVVLRLVGLLEG
                     LDDKDVLDLVAALSRLSPTTARRATRLMSGVVRTVTR"
     gene            5182..5532
                     /locus_tag="VIF63_04370"
     CDS             5182..5532
                     /locus_tag="VIF63_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238650.1"
                     /translation="MERRVQTVWLAGLLIGVLAGIIGLIIPVAGIGLAIASTLLVLLR
                     GPRTFGLGGVWLGFGGVWTALLFRALLECATTSTPSDGCHSQLFQTYIVVGLLMAVIG
                     LFVTVVAIRGRRAA"
     gene            complement(5529..>5826)
                     /locus_tag="VIF63_04375"
     CDS             complement(5529..>5826)
                     /locus_tag="VIF63_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238651.1"
                     /translation="DGRVHSAAKPPNDLGVVPAEAMAKLNAALQGTDFAAIKSHPFTG
                     ECPIAFDGQELVFDFAVGAGTQQIASCTTEIDWGHPLFVAVGVALGEWIPIPLT"
BASE COUNT          837 a         2023 c         2075 g          891 t
ORIGIN      
        1 tggccttcgt cgccgactcg cgccactcgc gctggcgtga cagctcgcgc gcctccctcg
       61 cccggggctc gggggccgga gtcgtccctc gctcgacggg cccgcgatcc gcgatctcac
      121 ccgcgacgaa cggcgcctcg acccgcggcg caccggcacc agcgccggcg gctgccgtca
      181 gcgagccgga cggcgaccgg ccgggccgcc acgatccgga cgcgcccggg cggaatccgg
      241 gcctcgaccc acgttcaccc ggaaaaaagc cagagccgga gccgccgcga ccgcccgggt
      301 cggcgcggta atcgccacgg aagtcgccgc ggaacccacc gtcaccacgc cacgacggct
      361 cggtccgcca cgagctgccc tggccgatga gcccggagaa tcgatcgggg gcgagctgct
      421 cccgaggcga cggacgcggc cgcgactgcg agcgatgcac gccctcggcg tcgcgccagc
      481 gcagctcggg cgattggaca tcgccgcgca ggatcgagtc gatggtctcc gcgacgtccc
      541 cgtgcaccag cacgcgctcg cggtcctgga tctcgatgac gacatcgaac gtcggcggcg
      601 ccttgcgctc gaggacgctc ttctgcgatc gccggcggcg tgcctcctcg tcgccgaggg
      661 tcacggactg gatgccgccg atcaggtcgg acagggtcgg gttcagcatg aggttgtcga
      721 gcgtgttgcc gtgcgccgtt ccgatcagct gcacaccgcg ctcggcgatc gtgcgcgcgg
      781 cctgcgcctc cagctccgtc ccgatctcgt cgatgacgat gacctggggc atgtggttct
      841 cgaccgcctc gatcatgacc tcgtgctgca gcgacggcgt gcggacctgc atgcgccggg
      901 ccttgccgat cgccggatgg gggatgtcgc cgtcgccggc gatctcattc gaggtgtcga
      961 cgacgacgac gcgcttgtcg aggtcgtcgg cgaggacgcg cgccgcctcg cgcagcatcg
     1021 tcgtcttgcc gatgccgggc ctgcccatga tcagcagcga cttcccggtc tcgatgaagt
     1081 cggcgatgat ctcgatcgtc ccgaacaccg cgcgaccgac gcgcagcgtc agcccgacga
     1141 ccttgccggt ccggttccgg atcgcgctga tccggtggag cgtccgctcg atcccggccc
     1201 ggttgtcgtc accgaagaca ccgatgtggt caaccacgtg ctgcaggtcg gcctcgacga
     1261 tctcgcgatc gagcagcgtc acctcggaat ccgggtagcg tgcctcgggc cgccggccga
     1321 ggtccatgac gacctcgatc agcgcctccc ggtcggtgag ggcgtgaatc gcggcgacga
     1381 tctcgggcgg cagcgcggcg agcaggacgt cgagatcgtc cgtcgcctcg tgcggctccg
     1441 tcgattcgcg tcgttccggc tgcggctcag gagccacggg tgacctccag gtgtcgaacc
     1501 gccgcaggaa cgaggctcgg ctcggcgacc cggacgaggg tctcgcgcat gagcagcgtg
     1561 acggtggcga tctcggcgaa ccccgattcc tcgagccggc ggttcgctgg tgattcgtag
     1621 gttcgcacgg gcgcaagaac gccgtgattc ggacggctca cgccgttctt ttcgacctgt
     1681 ccggcaatcg tcccgagccc gaactcgatg agtggcttga ggtcgatgtc gggccgcccg
     1741 agcggtcgca ggtagtgcgg ctggtcctcg cgcgcgagcc cggtctggat gaacgcgtcg
     1801 atgcgcgcgc catcggggga gtcctggacg aacccgtcga ggtcggcgaa ccgcaggatc
     1861 ggggcgagcg agctgcgggg cacgcgccag ttcgagccct ggcgctccca gtccgggatc
     1921 ctcagggcct cgaggcgctg gacgggcgcc ggcgtgaccg ccgagtacag ctgcatcagc
     1981 gcattcgcat cgaccgggcg cgcctggcgg atgcgcgcct tcgtcgcgtg ctcgtcggtc
     2041 cacggccggg gcagcggctg gtcgggcggt cgatacagga cgcgctcctc gccgtagcgc
     2101 atgaagcccg cctgcatgaa cagctcgacg ttgtcgtccg cgtccgcgca cgcgacgtgg
     2161 aagcggttgg cgccacgctt ctgcgcctcg cgcaggacct gttgcacgag gcgatggcgg
     2221 atctcacccg gctgcgcccc gccgatcgcg tcgagctcga cgatcgtcca gtcgtcgcgc
     2281 cagttctcgc gctccacccg caccaggccg gacacgtgcc ggtccgactc gtagacgaac
     2341 agcaggtcgt gcggccggaa cgcgacgagc ggcaggcgga acaggctgaa cacgccgatc
     2401 ggcgggccgt tcacgggcaa tcccagcgtg cgcgagccgg atccctccgt ctgggcgatg
     2461 cgcgacagct cgccgagcgc ggcgaggtcg gtcagtcgag cggcgcggac cttgcccgat
     2521 ggccggcccg gccggccatc cgcgacggtc aacgggtgag cccggctcga cgcgttgggt
     2581 ggtggcggcg cccggaccct tcgcccggat cgtcatgctc cgcggccatc gtcgcgatca
     2641 gccggtggaa gcgcggctcg tcggcgagct ccggatggaa cgcggtcgcg aggatgttct
     2701 tctcgcggac ggcgacgatt cgcccgtcgt cgaggcgggc aaggacgtcg acgcccggac
     2761 cgactcgctc gatgaccggc gcccggatga acacggcgtg gaccggctgg tcgccgacga
     2821 tcggcacgga cagctcgccc tcgaacgagt cgagctggcg cccgaaggca ttgcgccgga
     2881 ccgtgacgtc gagaagcggc aggatcggct cgtcaccgtc ggcgatctcc cgggacagga
     2941 cgatcattcc ggcgcacgtg ccgaagagcg gcgcgccggt cgcggcgaag tcgagcaacg
     3001 gctcgcgcag cgcccagcgc tcgatgagcc ggcgcatggt ggtcgattcg ccgcccggca
     3061 ggatcagccc gctgatgccc tcgaggtcgc tcgcgaggcg cacctcgacg gactcgacgc
     3121 cgatcgcgtc gagcatgcgg atgtgctccg cgaaatcccc ctggagtgca aggaccccga
     3181 tcttcacggc tgttgggccg cggccgcgct caggcggtcc gattgcaagg cggcaacgag
     3241 caccgaaagc gagcgcacct gccgggtgcg tgagcgcgga gcggagatgc caacgcagcg
     3301 agcgggccgc atcagcgcgg ccgggctcac cagccgcgaa cggccaggcg ctcgccctca
     3361 gggatcgctt ccatggcgat gcctcgcatc ggggcgccga ggcccttcga gacgtcggcc
     3421 aggatcttcg ggtcgtcgaa gtgggtcgtc gcctggacga tcgcgttcgc catgcgggcg
     3481 ggatcctcgc tcttgaagat gcccgagccg acgaacacgc cttccgcgcc gagctgcatg
     3541 accagcgccg cgtcggcagg ggtcgagatg ccacccgcga cgaagttcac gaccggcagc
     3601 ctgccgttcg cggcgacgtc cttgacgagc tcgaacggcg cctgcagctc cttggcggcg
     3661 acgaacagct cgtcatcgcg catcgactgg aggcgtcgga tctcggacag gacggagcgc
     3721 agctgcatca ccgcctggac gacgttgccg gtcccggcct cgcccttggt ccggatcatc
     3781 gcggcgccct cgttgatgcg ccgaagcgct tcgccgaggt tccggcagcc gcacacgaac
     3841 gggaccttga aggcgtgctt gtcgatgtgg ttggcctcgt cggccggggt caggacctcg
     3901 gactcgtcga tgtagtcgac gccgagcgcc tcgaggatct gggcctcgac gaaatggccg
     3961 atgcgggcct tcgccatcac cgggatggtg accgcgtcca tgatcccctg gatcattgcc
     4021 gggtcggaca tgcgggcgac gccgcccgcg gcccggatgt cggcgggcac gcgctcgagg
     4081 gccatgacgg ccaccgcgcc cgcatcctcc gcgatcttgg cgtgctcggc agtgacgacg
     4141 tccatgatga cgccgccctt gagcatctgg gccaggccct tcttggtcgt ccaggtggac
     4201 gtctctgggc tcatgtgttg gtggaacctc cgttcggacg ccgcgtgctg acacgcgccg
     4261 ggcggattat cgcacgccga tttggacgag ccgagtagtg cgtcggtcct gccgccgttc
     4321 ggattcggtt gcggcgctgc ggctagcgcg taacggttcg aacgaccccg gacatgagcc
     4381 gcgtcgcgcg gcgggccgtg gtgggcgaca ggcgcgagag cgcagcgacg agatcgagca
     4441 cgtccttgtc gtcgaggccc tcgagcagcc cgaccaggcg caggacgacg tcgtcatcga
     4501 cctcgccgag gagccggagc gcgcgctcca gctgtctggg ctcgaggcgt gggacgagcc
     4561 caaccgcccg atcgaacaat cgaacgaaga cgaggaacgt gctgagccat tcgtggagcg
     4621 caacgagctc cgggtcgccg gggtggcggc gcagctcgtc cgcggcctcg ccaccggagc
     4681 gttcgagcag gctgatgatc gggtcgccct cgcgggtctt gcgctcggcg acgacacggc
     4741 cgaaccagcg ccagtgatcc cccaccgcct gatacgccac gccggccggg ccccgccggc
     4801 cgacgcgccg cggctccgtc acccgctcga cgagacccca cgagaccgct tcgttgagcg
     4861 ccaggcttgc tgcacgatgc gacagcccaa gggcttcgcg gacctcgcgc tcggtgagcg
     4921 gctcctgccg ggtcatgaga tacgcctgca cacgcgcaat cgcgggcgcg acaccccagg
     4981 cggcgcccat ctcgccccac gcgtcggcga gccggatgcg gatcgcctca gcggactggg
     5041 aggccgggtt cgcgtcgtca cgtgcgcttg gcggggcggc cagctgcgca gcagatgaag
     5101 cggtggtcac cgcttgagag taccgcacag gacgagaact tgccacttga cgctagcaca
     5161 tttctaccgt agaaccgctc ggtggaacgg cgtgtgcaga ccgtctggct agccgggctg
     5221 ttgatcggcg tcctcgccgg gatcatcggt ctgatcatcc ctgtcgccgg catcgggctg
     5281 gcgatcgcgt cgacactgtt ggtgctgctg cgcggccctc ggacgttcgg actcggtggc
     5341 gtctggcttg gattcggtgg tgtctggacc gcgctgctgt tccgggcgct gctcgaatgc
     5401 gcgacgacgt ccacgcccag cgatgggtgc cacagccagt tgttccagac ctacatcgtg
     5461 gtcggcttgc tgatggcggt gatcgggctg ttcgtcacgg ttgtcgcgat tcggggtcgc
     5521 cgcgcggcct aggtcagcgg gatcgggatc cactctccga gcgccacgcc gaccgccacg
     5581 aagagcgggt ggccccaatc gatctcggtc gtgcacgagg caatctgctg ggtgccggcg
     5641 ccgacggcga agtcgaagac cagctcctgg ccgtcgaacg caatcgggca ttcgccggtg
     5701 aacgggtgcg acttgatcgc cgcgaagtcc gtcccctgga gtgctgcgtt gagcttggcc
     5761 attgcctccg caggcacgac gccgaggtcg ttgggcggct tggctgcgct gtggacgcgc
     5821 ccgtca
//
LOCUS       DATBSW010000158         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_14267, whole genome shotgun sequence.
ACCESSION   DATBSW010000158 DATBSW010000000
VERSION     DATBSW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14267"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VIF63_04380"
     CDS             complement(<1..660)
                     /locus_tag="VIF63_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8238652.1"
                     /translation="MSAAVLAARPRVGTRSIARRPLVLAGVGLATLAVALVAGVAAGS
                     VFVPPGDTVAILAYRLFGLDLGVTWTQAQETIVFDLRLPRVLTAMTVGMGLAVAGATF
                     QGVLRNPLADPYVLGTASGAALGAAIAVILPVRTLILEFGLLHGLAFLGALGAVWLVY
                     RLSRVGGHGQMTSLLLTGYAVASLLAAGLSMAMFVSGTGLRQIFSYLLGGFEAASWLR
                     FG"
     gene            complement(685..1692)
                     /locus_tag="VIF63_04385"
     CDS             complement(685..1692)
                     /locus_tag="VIF63_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8238653.1"
                     /translation="MAARPIPAPARLFLALLILIVGACTPVASTHAPTTAPATPTAAP
                     TGPPPTATPVPAFPVTLTDDEGTKVTIAAEPRKIVSLTPDTTESLFALGAGSRVVGKV
                     EDLANFPPEAASVPVVARFDGVDVEQIVALEADLVISAGTGLTQGDAVEQLRRANITV
                     LVSYPTTVDGGLASIRRIGQAIGLAEEAATLADGIAARLDDLTAIASSAGSRPRVFYE
                     IDVTGGIFTPPAASIYGEMFKLAGGEPISGDANYSISLEELIAADPEVILLGDAAYGA
                     TPENVVAREGWGGMTAVKDGRIVAVDDIVITRPGPRLAQGLEALIRAIHPELTLPSAA
                     P"
     regulatory      complement(1832..1904)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          347 a          762 c          809 g          289 t
ORIGIN      
        1 gccgaatcgc agccacgagg ccgcctcgaa gcccccgagc aggtacgaga agatctgccg
       61 cagtcccgtg ccggacacga acatcgccat cgacagcccg gccgcgagca gcgacgcgac
      121 ggcgtacccg gtgaggagca ggctcgtcat ctggccgtgc ccgcccacgc gggagagccg
      181 gtacacgagc cacacggcgc cgagcgcacc gaggaacgcc agcccgtgca gcagcccgaa
      241 ctcgaggatc agcgtgcgga ccggcaggat caccgcgatc gccgcaccca gcgccgcacc
      301 ggacgccgtg cccagcacgt acggatccgc gagcgggttc cggagcacgc cctggaacgt
      361 ggcgcccgcg actgcgagcc ccatgcccac cgtcatcgcg gtgaggacgc gcgggaggcg
      421 gaggtcgaac acgatcgtct cctgggcctg cgtccaggtg accccgaggt cgagcccgaa
      481 caggcgatag gcgaggatcg ccaccgtgtc accgggcggc acgaagaccg acccggcggc
      541 aacgcctgcg acgagtgcga ccgcgagcgt cgccaacccc acccccgcaa ggacgagcgg
      601 tcgacgggcg atcgaccgcg tcccgacgcg ggggcgggcg gccaggacgg cggcgctcaa
      661 agagcgacgc tcacgaagcc gcgctcatgg cgccgcgctg gggagcgtca gctcagggtg
      721 gatcgccctg atcagggcct ccaaaccctg ggcaaggcgc ggtccgggcc gcgtgatgac
      781 gatgtcgtcg acggcgacga tccggccatc cttcacggcg gtcatcccgc cccagccctc
      841 gcgggccacg acattctcgg gagttgcgcc gtatgccgca tcgccgagca ggatcacctc
      901 cgggtcggcg gcgatcagct cctcgagcga gatcgagtag ttggcgtcgc ccgagatcgg
      961 ctcgccgccc gcgagcttga acatctcgcc atagatcgac gcggccggcg gggtgaagat
     1021 cccacccgtg acgtcgatct cgtagaagac acggggccgc gagccggcgc tcgaggcaat
     1081 cgccgtcagg tcgtcgagcc gcgccgcgat gccgtcggcg agcgtcgccg cttcctcggc
     1141 gagcccgatc gcctgcccga tgcgcctgat gctggcgagc ccgccgtcga ccgtcgtcgg
     1201 atagctgacg agcacggtga tgttggcgcg gcgcagctgc tcgaccgcgt cgccctgggt
     1261 caggccggtg ccggccgaga tcacgaggtc cgcctcgagg gccacgatct gctcgacgtc
     1321 gacgccgtcg aacctcgcga cgaccggcac ggacgcggct tctggcggga agttcgcgag
     1381 gtcctcgacc ttgccgacga cgcgcgaccc ggcaccgagc gcgaagagcg actcggtggt
     1441 gtccggggtc agcgacacga tcttcctggg ttcggcggcg atcgtgacct tggtgccttc
     1501 gtcgtcggtc agcgtcaccg ggaaggcggg gaccggggtc gcggtcggcg gcggaccggt
     1561 gggcgcggcg gttggcgtcg ctggtgcggt ggtcggggca tgtgtcgagg caaccggcgt
     1621 gcatgcgccg acgatcagga tgagcagcgc aaggaacagg cgcgcgggtg cgggaatcgg
     1681 acgggcagcc atggaagtct ccggcgtggg ggtctgggac cgggcgcgga acggcgccac
     1741 gggacgtcgg ggagggctgc ctcagccggt ggaacgcatg tggtgcctac cctttctctc
     1801 gaaggtcgag caagccactg cgggtcggcg accggacttc cattccgggc gatccctcgc
     1861 cctcgatggt caccgtagcg ggactgtgcc ggactctcac cggcttcgcg gccgcgcagt
     1921 gtgtcgtctt gagacgacgg ccgaagccta gcactccagt acccgaattg ggcccaggtg
     1981 agccgccgat aagcggccgg tgatacctcg atgactgcgc atgcagcccg ggagacgaag
     2041 atgccgtcgt catcgcctcc cgccggcaaa cccgccgcgg aggtcgagat gtcgggacgt
     2101 cgctggtcac tgtggctcgg gccgccgatc gccctcgttg cggtcgtgct gctgctcggg
     2161 cagctccaac cgagggcgga ggccggtggt cggatgacgg cgccgcc
//
LOCUS       DATBSW010000159         7836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1107603, whole genome shotgun sequence.
ACCESSION   DATBSW010000159 DATBSW010000000
VERSION     DATBSW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107603"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..586
                     /locus_tag="VIF63_04390"
     CDS             83..586
                     /locus_tag="VIF63_04390"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8238654.1"
                     /translation="MSRIVEEIEVEVPVRIAYDQWTQFESFPKFMEGIDRVFQVDDTT
                     LEWTATIAGQVKHWRAEIVEQRPDGLVAWRSVDGARNDGEVRFEPRGQDKALITLQLD
                     VEPEGLIEKAGDALGLVERRVRSDLERFKEFIEDRGTPTGGWRGRVDDGEVKVGSGGT
                     AGTEVDR"
     gene            complement(819..1178)
                     /locus_tag="VIF63_04395"
     CDS             complement(819..1178)
                     /locus_tag="VIF63_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238655.1"
                     /translation="MTSESTTTSESTTTRRRSTNGADTTERGIQAAVAEVRGALENVG
                     RSMPDVARASRQSIDDMFKAIETGSDERVSAGVTLSLGLAIGMLLGGAPRLLILAALA
                     PVVAMGLVLQDRRKQPA"
     gene            1322..1546
                     /locus_tag="VIF63_04400"
     CDS             1322..1546
                     /locus_tag="VIF63_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238656.1"
                     /translation="MSDLSKQARDAGADVKEAWRKADGDESLEDKAKNAGDRISNAVK
                     DAGDKVHQEADEMSREAAYDKGRADEMARR"
     gene            complement(1474..2163)
                     /locus_tag="VIF63_04405"
     CDS             complement(1474..2163)
                     /locus_tag="VIF63_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238657.1"
                     /translation="MDEIISDFFASPVVGAAGTALAGTVVALWLAAAWWAYRDAGRRT
                     ESTLAGYLASFWIVVATPLFLPFALVIYRVARPQVSAAEQRASALARELSATHAADPS
                     CPACAASVDAAWLRCPDCATWLAAPCTACGRWSDPTFDLCPWCGAEPQRGSPEPQRAP
                     DPQRAPEPQWAPVPAAAAAAAAPAGFPAHGMAAADALSAAAMLSGRQRRAISSARPLS
                     YAASRDISSAS"
     gene            complement(2278..3474)
                     /locus_tag="VIF63_04410"
     CDS             complement(2278..3474)
                     /locus_tag="VIF63_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238658.1"
                     /translation="MALIASAFAMLGRFVGRVLTTTLGWASILLFGRVPQDRQVWLAV
                     LTFGSLAWVAAVVGIVIPDLGTVLLAAVPLPDWVPDELISLAMLAVALVLPAVLGGVT
                     LLLQDPEDRPKGFAMVESVGRGYLLVPTLAVTIVVLAIAGTLRKLDSLVHRREDAHIP
                     IVVRPGRYDALVDTLEETLRGGELVNARGAGSAILTIPARVLARVAGGGIRGLVPDRL
                     VELRGPSVRLAIYPSDLALTGGKDAVAKARALVARDVSSRDAWFTTTREAQKVEDRIA
                     ALETADGATLARELPALDRRLLDLTVDQDTFEVLYRRRLQLAVSPIDDVRAADEPEPD
                     ERTVPAHGRVSLGSIVGLVVAALAALDLAFAALQGRERRRQVDASGDRDGRVAQRWIR
                     RIRGIA"
     gene            complement(3539..4576)
                     /locus_tag="VIF63_04415"
     CDS             complement(3539..4576)
                     /locus_tag="VIF63_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238659.1"
                     /translation="MSPDDPGILRRRAGRGFTYISPAGRRITAERTLERIRAIVIPPA
                     WTDVWICPDPNGHIQAVGNDARGRRQYRYHPAFRARRDRAKFDRLIRFGKALPRIRRQ
                     VRADLRRHGLPRPKVLAAVVRLLELTRLRVGNQEYARLNRSFGLSTLRERHATVSGAT
                     IHFRFRGKGGRAVERSFVDRGLAAVVRRCQDLPGQALFQYLDEAGEPQAISSEDVNAY
                     LRDAAGSEEFSAKDFRTWTATLATFLAIRSAEAAQPGDRAPSKSTVDEAVRTAAADLG
                     NTTAVTRASYVHPAVLRLPGGEDVRSRRPDTLPTRRDELALLAALRAAGATRSQAQKA
                     RLRQRPAGRAA"
     gene            complement(4642..5943)
                     /locus_tag="VIF63_04420"
     CDS             complement(4642..5943)
                     /locus_tag="VIF63_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8238660.1"
                     /translation="MSQDHGALGRPAPDNGTLPGAVPGRSVTGAAIPRLRIAQVAPPV
                     ERVPPEAYGGTERIVHELTTELVRRGHDVTVFASGDSDIPAPARLFPTVEKALRPAGI
                     EADSGPYFAATVETVRKHASEFDVIHSHLEWWSWPLARDSAVPVVSTFHGRLDLPWAD
                     RLFTSESVGMVAISRHQASTHPDVPWTIIHNGLRLDDAPFVEQPGDEFCFVGRVDPEK
                     GVVEAMDIAQRAGRRLRIAAKVGNMGRQRAYYDDVFLPALKKAGSSVEYLGEVQPAER
                     DQLFAQSYATLMPGAWPEPFGLVSIESLACGTPVLARRVGGLPEIIREGVDGFFGDDA
                     AAIAFYADRVGDLDRRAIRERVLDRFSAARMADRYEELYVRMLARAGATEPSEDVAAR
                     ATEPTPASKSAPTKPRPARPGSSPAPAGRRGVLARLLGGRT"
     gene            6224..6982
                     /locus_tag="VIF63_04425"
     CDS             6224..6982
                     /locus_tag="VIF63_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8238661.1"
                     /translation="MSTQPLVLVADDEPRITKLVAIALSEEGFRVVTANGGEQALQKA
                     EEVRPDIVLLDIVMPDLDGIEVMRQLRERRPVPVILLTAKGSTADKAKGLDLGADDYI
                     AKPFHPDELAARVRAVIRRSGGAEPGAGVIKFDDVEIDLERRMVTRDGELVQLSRTEW
                     LLLQHLAANAGKVVLHTELLTKVWGPEYRDDLQYLRVWVSRVRRKLGALPGEPGRIRT
                     FQGIGYLLDVEGATQKHQGASEDVAEREEPALIG"
     gene            complement(7324..>7836)
                     /locus_tag="VIF63_04430"
     CDS             complement(7324..>7836)
                     /locus_tag="VIF63_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY8238662.1"
                     /translation="SDAAGRVHILEMRDAPLGADILQELTARLATLDDGAVVGFLGVT
                     RRSPGTPAPGQAGEAARHAGRAVDALEYEALPEMALRVMNEIANEIEARFGVTRLAIV
                     HRTGEVPLGEASVAIVTCSPHRDAAFQAARYAIDETKARAPIWKAEHFADGHVWIGEP
                     ARDSAPGTEP"
BASE COUNT         1182 a         2726 c         2861 g         1067 t
ORIGIN      
        1 aacccgccgg caacacaacc gcagacgccc accggacccc cggtgggcgt ctgcatgtca
       61 cccctatgga aggagggacg tcatgtcccg catcgtcgaa gagatcgagg tcgaggtgcc
      121 agtgcggatc gcctacgacc agtggacgca gttcgagtcg ttcccgaagt tcatggaggg
      181 catcgaccgc gtcttccagg tcgacgacac cacgctcgag tggaccgcga ccatcgcggg
      241 ccaggtgaag cactggcgcg ccgagatcgt cgagcagcgc cccgacgggc tcgtcgcctg
      301 gcggagcgtc gacggcgccc gcaacgacgg cgaggtgcgc ttcgagcccc gtgggcagga
      361 caaggcgctc atcacgctcc agctcgacgt cgagccggaa gggctcatcg agaaggccgg
      421 cgacgcgctc ggcctggtcg agcggcgcgt ccgcagcgac ctcgaacgat tcaaggagtt
      481 catcgaggac cggggcacgc cgaccggcgg ctggcgcggc cgcgtcgacg atggcgaggt
      541 caaggtcggc tccggcggca cggccgggac ggaggtcgac cgctagctgg cccgccagcc
      601 atcgaagggg tagcgcccgc gctcgatctc cgggggagcg actgcgatgc ggctgcaatg
      661 tcgcttgccg acgggcggta cgtcgcgttg acggccgctc ggccacgatc aacccgacgc
      721 ggcgatccct cgtcgccacg ttccctccct tgggatcccg gtctgcccgg ccggactccc
      781 gccctcctcg accgtccgtc ccaccggacg gtcgcgccct atgcgggctg cttgcgccgg
      841 tcctggagga ccaggcccat cgccacgacg ggcgccaacg cagcgaggat cagcaggcgc
      901 ggggcgccgc cgagcagcat cccgatggcc aggccgagcg acagcgtgac gccggccgag
      961 acgcgctcgt cggagccagt ctcgatcgcc ttgaacatgt cgtcgatcga ctggcgtgac
     1021 gctcgcgcga catcgggcat ggaccggccg acgttctcga gcgcgccgcg gacctccgcg
     1081 acggcggcct ggatgccgcg ctcggtcgtg tcggcgccgt tggtggaacg acgacgggtc
     1141 gtggtggatt cgctggtcgt ggtcgattcg ctggtcatgg cgctacctcc gaaattggtg
     1201 cggcgaggcc gctgcgatcg ggttgcaact caacgggcag cacgcatgag gcatcgagga
     1261 gctgccgatg ggtaggttca agtcgagcac ggcgcgtgcc gtgccacaag gaggacgtga
     1321 gatgagtgac ctcagcaaac aggctcggga cgccggcgca gacgtcaagg aagcttggcg
     1381 caaggccgac ggtgacgagt cgctcgagga caaggccaag aacgcgggtg accgcatcag
     1441 caacgccgtc aaggacgccg gcgacaaggt gcatcaggaa gcggacgaga tgtcccgcga
     1501 agcggcgtac gacaagggtc gcgccgacga gatggcccgt cgctgacgcc cggacagcat
     1561 ggccgccgcg gacagcgcgt ctgcggcggc cattccatgt gcggggaaac cggcgggtgc
     1621 ggccgcagcc gcggcggcgg ccggcacggg cgcccactgg ggctcgggag cgcgctgcgg
     1681 gtcgggcgct cgctggggct cgggggagcc gcgctgcggc tctgcgccgc accacggaca
     1741 aaggtcgaag gtcgggtcgg accacctgcc gcaggcggtg cacggggcgg ccagccatgt
     1801 tgcgcagtcg gggcagcgca gccaggcggc atcgacgctc gccgcgcagg cggggcagct
     1861 cggatccgcg gcatgcgtcg cggagagctc acgggcaaga gcggacgcgc gctgctcggc
     1921 agcggacacc tggggccgcg ccacgcggta gatgaccagc gcgaacggca ggaacagcgg
     1981 cgtggcgacg acgatccaga acgaggcaag gtacccagcg agggtggatt cggtgcggcg
     2041 ccctgcgtcg cggtatgccc accacgcggc agccagccac agcgccacga cggtgccggc
     2101 gagggccgtg ccggcggcgc cgaccacggg gctcgcgaag aagtcggaaa tgatctcgtc
     2161 catttgcggg tcagtttggg gtcggatccg tctccgccat ggatgtcgcg tctcctcgga
     2221 ccttgcagat gcacgacgcc ctgcgtgcgt cgccggccga cgcgtccccg ggatggatca
     2281 ggcgatccct cgaatcctgc gaatccatcg ctgcgccact cgcccatcgc ggtcgccgga
     2341 cgcatcgacc tgccgccggc gttcgcggcc ctggagcgcc gcgaaggcga ggtcgagggc
     2401 ggcgagggcg gcaacaacca gcccgacgat cgagccaagc gacacgcggc catgggccgg
     2461 cacggtgcgc tcgtccggct cgggctcgtc agccgcgcgc acatcgtcga tgggtgacac
     2521 ggcaagctgc agccggcggc ggtagagcac ctcgaaggtg tcctggtcga cggtgaggtc
     2581 gagcagccga cgatcgagcg cgggcagctc gcgggcaagg gtcgcgccgt cggccgtctc
     2641 cagcgcggcg atccgatcct cgaccttctg ggcctcgcgc gtcgtcgtga accaggcatc
     2701 gcgtgacgac acgtcgcgcg cgacgagcgc ccgggccttc gcgacggcat ccttgccccc
     2761 cgtcagcgcg aggtcgctcg ggtagatcgc gaggcggacg ctcggtcccc gaagctcgac
     2821 caggcgatcc gggaccagtc cccggatgcc acccccggcg accctcgcca ggacgcgcgc
     2881 cgggatggtg aggatcgccg agcccgcgcc tcgagcgttc acgagctcgc cgccgcgcaa
     2941 cgtctcctcc agcgtgtcca ccaacgcgtc gtagcggccg gggcgcacga cgatggggat
     3001 atgcgcgtct tcgcggcggt gcaccagcga gtcgagcttg cggagcgtgc ccgcgatggc
     3061 gaggacgacg atcgtcaccg cgagcgtggg cacgagcagg tagccgcgcc cgaccgactc
     3121 gaccatcgcg aagcccttcg gccgatcctc cggatcctgg aggaggagcg tgacgccccc
     3181 cagcaccgcc ggcaacacga gtgcgacggc gagcatcgcg aggctgatga gctcgtcggg
     3241 gacccaatcg ggcagcggta cagcggcgag caggacggtt ccgaggtccg gaatcacgat
     3301 gccgacgacg gcagcgaccc aggccagcga gccgaaggtc agcacggcaa gccagacctg
     3361 ccgatcctgg ggcacccgcc cgaacagcag gatcgacgcc cagccgagcg tggtcgtcag
     3421 cacgcggccg acgaagcgcc cgagcatcgc gaacgcagag gcgatcaggg ccatcgaggt
     3481 cgctccctcc gaccgaagtc gttcgtcgtg gcgcggtctc gccgcgcata cgccaaacct
     3541 aggccgcgcg acccgccggg cgttgccgca gtcgagcctt ctgcgcttgc gacctcgttg
     3601 cacccgcggc ccggagggca gcgaggagcg cgagctcgtc gcggcgcgtg ggcaacgtgt
     3661 cgggccggcg cgagcggacg tcctcgccgc cgggcagccg gagcaccgcg ggatgcacgt
     3721 agctcgcgcg cgtgacggcc gtggtgttgc cgaggtccgc ggcggccgtt cgaaccgcct
     3781 cgtcgacggt cgatttcgaa ggcgcgcggt cgcccggctg ggcggcctcg gccgaccgga
     3841 tggcaaggaa cgtggcgagt gttgccgtcc acgtccggaa gtccttcgcg ctgaactcct
     3901 cgctgcccgc ggcgtcgcgg aggtaggcat tcacgtcctc cgacgagatc gcctggggct
     3961 cgcccgcttc atcgaggtac tggaagaggg cctggccggg aaggtcctgg cagcggcgga
     4021 cgactgccgc gaggccacga tcgacgaagc tccgctcgac agccctgccg cccttgccgc
     4081 ggaaccggaa atggatcgtc gcgccgctga cggtcgcgtg gcgctcgcgc aaggtcgaca
     4141 gcccgaacga gcggttcagc cgcgcgtact cctggttccc gacgcgcagt cgcgtcagct
     4201 cgagcaggcg gacgacggct gcgaggacct tgggccgggg cagcccatgc ctgcgaaggt
     4261 cggcgcggac ctggcgccgg atccgcggca gcgccttgcc gaaccgaatg agccgatcga
     4321 atttcgcgcg atcgcgtcgg gcgcggaagg ccgggtggta tcgatactgg cggcggccgc
     4381 gcgcgtcatt gccgaccgcc tggatgtggc cgttcgggtc ggggcagatc cagacatcgg
     4441 tccaggccgg cgggatgacg atggcccgga tgcgctccag ggtccgttcc gcggtgatgc
     4501 gacgtccggc gggggagata taggtgaacc cgcgtccggc tcgccgccgg agaataccgg
     4561 ggtcgtcggg gctcacgtat cggagcccga ctgcggtcgc gtcccggcgc gccggggccg
     4621 atcgagccgg gggcgtcgtg gtcaggttcg accgccgagc aggcgggcga gcacgccacg
     4681 ccggcccgcc ggcgcgggtg acgagccggg cctcgccggc cgcggcttgg tgggggccga
     4741 tttgctcgcg ggcgttggct ccgtcgcccg tgctgcgaca tcctcgctgg gctcggtcgc
     4801 gcctgcccgc gcgagcatgc gcacgtagag ctcctcgtag cggtcggcca tgcgcgcagc
     4861 ggaaaaccga tcgagcacgc gttcgcggat tgcgcgtcgg tcgaggtcgc cgacccgatc
     4921 cgcatagaac gcgatggctg ccgcgtcgtc gccgaagaac ccgtcgaccc cctcccggat
     4981 gatctcgggc agcccgccga cgcggcgcgc gaggaccggc gtcccacacg cgagcgattc
     5041 gatgctgacg agcccgaacg gctcgggcca ggcgccgggc atcaacgtcg cgtagctctg
     5101 ggcaaagagc tggtcgcgtt ccgccggctg aacctcgccg aggtactcaa ccgacgaccc
     5161 cgccttcttg agcgccggca ggaagacatc gtcgtagtac gcgcgctggc ggcccatgtt
     5221 gccgaccttc gcggcgatgc gcaggcgacg cccggcgcgc tgggcgatgt ccatcgcctc
     5281 gacgacgccc ttctccgggt cgacccggcc gacgaagcag aactcgtcgc ccggctgctc
     5341 gacgaacggc gcgtcgtcga ggcgcaggcc gttgtggatg atcgtccagg gcacatcggg
     5401 atgggtgctg gcctggtgcc ggctgatggc caccatgccg acggactcgc tggtgaacag
     5461 ccggtcggcc cacggcaggt ccagccgtcc gtggaacgtc gagacgaccg ggaccgccga
     5521 gtcgcgcgcg agcggccacg accaccattc gaggtgcgag tggatgacgt cgaattccga
     5581 cgcgtgcttt cgcacggtct cgaccgtggc cgcgaagtac gggccggagt cggcttcgat
     5641 gcccgccggg cggagggcct tctcgacggt cgggaagagt cgtgccggcg ccgggatgtc
     5701 cgagtcgcca ctggcgaaga ccgtgacgtc atggccgcgt cgaaccagct cggtggtcag
     5761 ctcgtggacg atgcgctcgg tgccgccata cgcctcgggc ggcacgcgct cgaccggtgg
     5821 cgccacctgg gcgatgcgca gccgcgggat ggcggcgccg gtcacggatc ggccgggcac
     5881 tgcgccgggc aatgttccgt tgtccggcgc gggtcggccg agcgctccgt ggtcctggct
     5941 cacggcccct cctgttcgat cgggccgttg ctgcccgtca ttcgtcgcgt ccagcctagt
     6001 ccggtttccg tggcgtgccc ggtagcgcga ttcgggaccg ccgtcacagg cgtatatcgc
     6061 gctgggccgc tctggccatc gaccgatcgc cggccatgcg ttgtaatgcg gacgtaacgg
     6121 ttttgcaatg tccggccgcc atggccggga ggaacggatc gcgacaatcc ggccggtcag
     6181 tcgacattcg acagcaacgc tgatcaccga ggagatgcct cccgtgtcca cccagcctct
     6241 cgtgctcgtt gccgacgacg agccgcgcat caccaagctc gttgccatcg ccctgtccga
     6301 ggagggcttc cgcgtcgtca ccgccaacgg tggcgagcag gcactccaga aggccgagga
     6361 ggtccgcccc gacatcgtcc tgctggacat cgtgatgccc gacctcgacg gcatcgaggt
     6421 catgcgccag ctccgcgagc gccggccggt gccggtgatc ctgctgacgg cgaagggctc
     6481 gacggccgac aaggccaagg gcctcgacct cggcgccgac gactacatcg ccaagccgtt
     6541 ccacccggac gagctcgcag cgcgtgtccg cgcggtcatc cgccgctccg gcggagccga
     6601 gcccggcgcg ggcgtcatca agttcgacga cgtcgagatc gatctcgagc ggcgcatggt
     6661 cacccgcgac ggcgagctcg tgcagctctc ccgcaccgag tggctgctgc tccagcacct
     6721 ggccgcgaac gcgggcaagg tcgtcctgca caccgagctc ctgacgaagg tctgggggcc
     6781 cgagtatcgc gacgacctcc agtacctgcg cgtgtgggtc tcgcgcgtcc ggcggaagct
     6841 cggcgcactg ccgggcgagc caggccggat ccgcaccttc cagggcatcg gctacctgct
     6901 cgacgtcgaa ggcgcgacgc agaagcacca gggcgcgtcc gaggacgtcg ccgagcgcga
     6961 ggagccagcg ctcatcggct aaccatcgat cggctcgagc catcctgggc tcgcgccacc
     7021 gccgaattca ccaacgccgc cgggtacgga cgctcccggc ggcgttttct cattccctgg
     7081 cccgtttccg cgggtttggc gcccttccga ccgctcacgc gccatatgca cgcatggggc
     7141 acgttcggag acgaatccag cgcgagcggg cctgattcga accggccgcg actgcggcgc
     7201 cgatcagccg aggtcccgcg tgggaggtcg acggagcgat tccgtccgaa tccgagggtt
     7261 ctccatcgcc aacgtgcgcc aggagcgcaa atggcgcagg ttcgcggtga gcgatcgctc
     7321 cgactagggc tcggtccctg gcgcagagtc gcgtgccggc tcgccgatcc agacgtggcc
     7381 gtccgcgaag tgctcggctt tccagatcgg ggcgcgggcc ttcgtctcgt cgatcgcgta
     7441 gcgggcggcc tggaacgccg catcccggtg cggggagcag gtgacgatcg cgaccgacgc
     7501 ctcgccgagc gggacctcgc cggtgcggtg gacgatcgcc agacgggtga ccccgaagcg
     7561 ggcctcgatc tcgttcgcga tctcgttcat cacccgcagc gccatctcgg gcagggcctc
     7621 gtactcgagc gcatcgacgg cccggccggc gtggcgcgct gcctcccctg cctggcccgg
     7681 cgcgggcgtg ccgggcgagc gccgggtgac cccgaggaac ccgacaacgg cgccgtcgtc
     7741 gagcgtcgcg agtcgggccg tgagctcctg gaggatgtcg gcgccgagcg gcgcgtcgcg
     7801 catctcgagg atgtgcacgc gaccggctgc gtccga
//
LOCUS       DATBSW010000160         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_190907, whole genome shotgun sequence.
ACCESSION   DATBSW010000160 DATBSW010000000
VERSION     DATBSW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190907"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="VIF63_04435"
     CDS             complement(<1..1812)
                     /locus_tag="VIF63_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8238663.1"
                     /translation="MPRTPIGRVLLVVAITAIVGVGSVAGLGVAVAAGVVGSLADGLP
                     DPADLDSLTFAQPTIVYDRTGKVELARFEREKRRVVTFDEVPQLVLDSTTTAEDRTFW
                     ENSGYDPVAIAAAAVQNATDEAQRERGASTITQQLVRARLLPPEVLEGGDRYLRKALE
                     IVQAARLTAAFPGEAGKQKIMTAYLNEIYYGHEAYGIAAAAEIYFGVTDLAKLTPAQA
                     ALLAGLPKAPSVYDPYRYAVKDDEDRLVVPAGAPAVVRRDYVLRGLAESRWTRLTPAQ
                     LQAALDEPVVLAGDKPTYMRAPHFSWAVRAELERILGGAEAVETGGYRVITTLDWDAQ
                     QLAERYLTAGAIIPNLSRGKGEDLIARMKFSKADKAWIRALRGKDIHNGALVALDYRT
                     GDVLAYVGSAGYYRDDLAGPKFAPQHDAAAAGRQPGSAFKVIVYSTAFEERELTPGSL
                     LLDISTDFGGGWSPNDADELERGPVLVRGAIQQSLNLPAVRALERVGNKAVADVAERF
                     GIRFMGGRDAFLQAGLAGAIGTVETRPIDLAGAFGSLGNGGVHEPTRMVLSITGPNGD
                     DVYEAGAPDGAEAVSKETAFLISDILAGNTDRRQNRFW"
     gene            complement(1921..2124)
                     /locus_tag="VIF63_04440"
     CDS             complement(1921..2124)
                     /locus_tag="VIF63_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238664.1"
                     /translation="MSAIPIAPTPARTLIWRLESGGWSRREASNLVALANGLRPASTG
                     WTVREIEHLRFLRAVVQSGRIDR"
     gene            2244..>4243
                     /locus_tag="VIF63_04445"
     CDS             2244..>4243
                     /locus_tag="VIF63_04445"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY8238665.1"
                     /translation="MAHPSIADAAAQAVPPIPPSQLPPPNPTIVPATDLGGVGVLKHG
                     NLYFLSDPFGDVHPDSRGLGLYDLDTRVLSCAILRINGVRPTLLRAQSAASHLSTIQL
                     TNPEQRRDPASKHTAGEALAMRAISVMRRRWIAGGLAERIEVTNYSADAQEIELELEL
                     DVDAADIFEVRGRVREHRGRFRPTLATPESLVFGYEGLDGLVRRTLISFSPGAVTAGG
                     RADGSSESGPAAGAASGGGGVLAGTSGSAIVRWTLVIEPGGRAGIHWEVTTDLEPMPG
                     AVPAEATPTRLARSVTSGQPDDGSAAVAEPTVAEAEYGAWDERCARIRSDGELLDLAI
                     RRSIADLRLLRNDGPFQGEHYVAAGIPWFATLFGRDSIITSLQVVPFMPDVARETLRV
                     LADWQATEDDPERDMEPGKILHELRVGELARTGELPHRPYFGSIDSTPLWLVLLDETY
                     RWTGDLELVRSLWPNALAALEWIDKYGDRDGDGFVEYERRTPHGLLNQGWKDSGDAIR
                     HRDGSVAVAPIALVEIQGYVYDAKRRMASLAERLGDGELAARLNEEASTLARRFDEAF
                     WMPDVGYYAIALDRDKHQVGTISSNPGHCLWSGIVPAERADAVVNHLLDPSMDCGWGI
                     RTYAAGQPGYNPVGYHTGSVWPHDNALIAAGMKRVGRHDAADR"
BASE COUNT          612 a         1492 c         1528 g          611 t
ORIGIN      
        1 ccagaaccgg ttctggcggc ggtcggtgtt gccggcaagg atgtcgctga tgaggaacgc
       61 ggtctccttc gagacggcct cggcgccatc cggcgcgccg gcctcgtaga cgtcgtcacc
      121 gttcggcccg gtgatcgaga ggaccatccg cgtcggctcg tggacgccgc cattgccgag
      181 gctgccgaac gcacccgcga ggtcgatcgg gcgcgtctcc acggtgccga tcgcgccggc
      241 gagcccggcc tgcaggaacg cgtcccgccc gcccatgaac cggatgccga accgctccgc
      301 gacgtccgcc acggccttat tgccgacccg ctcgagtgcg cgcaccgcgg gcaggttcag
      361 cgactgctgg atcgcgccgc ggaccagcac cgggccgcgc tcgagctcgt cggcgtcgtt
      421 gggcgaccag ccaccgccga agtcggtcga gatgtcgagc agcaggctgc ccggggtcag
      481 ctcgcgctcc tcgaacgccg tcgagtagac gatgaccttg aacgccgagc cgggctggcg
      541 cccggccgcc gccgcgtcgt gctggggcgc gaacttcggg ccggccaggt cgtcacggta
      601 atagcccgcg ctgccgacat aggccaggac gtcgccggtc cggtagtcga gggcaacgag
      661 tgcgccgttg tggatgtcct tgccgcgcaa ggcccggatc caggccttgt ccgccttcga
      721 gaacttcatc ctggcgatga ggtcctcgcc ctttccgcgc gacaggttcg ggatgatcgc
      781 gccggcggtg aggtaccgct cggcgagctg ctgcgcatcc cagtcgagcg tcgtgataac
      841 ccggtagccg ccggtctcga ccgcctcggc gccgccgagg attcgctcga gctccgctcg
      901 caccgcccac gagaaatgcg gggcgcgcat gtatgtcggt ttgtcgccgg caagcacgac
      961 gggctcatcg agcgcggcct ggagctgcgc cggcgtgagc cgtgtccagc gtgattcggc
     1021 gagcccgcgg agcacgtaat cgcgacgcac gaccgcgggc gcgccggcgg gcacgacgag
     1081 ccggtcctcg tcgtccttca ccgcataccg gtatgggtcg tagaccgagg gcgccttcgg
     1141 caggccggcg agcagcgctg cctgggccgg tgtgagcttc gcgaggtccg tcacgccgaa
     1201 gtagatctcg gccgcggcgg cgatcccata ggcctcgtgg ccgtagtaga tctcgttgag
     1261 gtacgcggtc atgatcttct gcttgccggc ttcacccggg aaggcggcgg tcaggcgagc
     1321 ggcctggacg atctccaggg ccttgcgcag gtaccgatcg ccaccttcga ggacttccgg
     1381 cggcagcagc cgcgcgcgca cgagctgctg cgtgatcgtc gacgcgccac gctcgcgctg
     1441 tgcctcgtcg gtcgcgttct ggacggcagc cgcagcgatc gcaaccgggt cgtagccgct
     1501 gttctcccag aacgttcggt cctcggccgt ggtcgtgctg tcgaggacca gctggggcac
     1561 ctcgtcgaag gtcaccacgc ggcgcttctc gcgctcgaag cgggccagct cgaccttgcc
     1621 ggttcggtcg tagacgatcg tcggctgggc gaacgtcagg ctgtcgaggt ccgcgggatc
     1681 gggcaggccg tccgcgaggg acccgaccac gccggccgcg accgcgacgc caaggcctgc
     1741 caccgaaccc accccgacga tcgcggtgat cgcgaccacg aggagcacgc ggccgatggg
     1801 cgtgcgcggc acgacgacgc gtcgggcggc gcctcgccgc agcaggacgg cgcgagtgcg
     1861 tggattgagt cgtagcgcgc ccttcccgct gggaagccgt cgcggcgagc ctgacaccgg
     1921 ctagcggtcg atccggccgg actgcaccac ggctcggagg aatcggaggt gctcgatctc
     1981 gcgcacggtc caacccgtgc tcgcgggccg gagtccgttc gcgagggcga cgaggttgga
     2041 cgcttcgcgc cggctccagc cgcctgattc gaggcgccag atcagggttc tggccggagt
     2101 cggggcgatc gggatggcgg acacgagccg agcgtagggt ggcgaccctg cgctccaccc
     2161 cacgaatggc tgtcacgttg ttgcagtccg ctgacacggc gccgtgccat gacaggcacc
     2221 gacgcgacgg tcatcatggg ccagtggcgc atccatcgat cgcggacgcg gctgcccagg
     2281 cagtgccgcc gatcccgccg agccagctcc cgccacccaa cccgaccatc gtcccggcga
     2341 cggacctcgg cggcgtgggc gtgctcaagc acggcaacct gtacttcctt tcggacccgt
     2401 tcggcgacgt ccatcccgat tcgcgcgggc tcggcctgta cgacctggac acgcgcgtgc
     2461 tctcgtgcgc catcctccgg atcaacggag tccgcccgac gctgctccga gcgcagtccg
     2521 cggcgagcca cctgagcacg atccagctga cgaacccgga acagcgccgg gatcccgcga
     2581 gcaagcacac ggccggcgag gcgctcgcga tgcgcgcgat ctcggtcatg cgccggcgct
     2641 ggatcgccgg cggcctcgcc gagcggatcg aggtgacgaa ctacagcgcg gacgcccagg
     2701 agatcgagct ggagctcgag ctcgacgtcg atgccgccga catcttcgag gtccgcggcc
     2761 gggtccgcga gcaccgggga cggttccggc cgacgctcgc gacgcccgaa tcgctggtct
     2821 tcggctacga gggcctggac gggctcgtcc ggcgaaccct gatctccttc tcgccgggtg
     2881 ccgtcaccgc gggcgggcga gccgacggga gctcggagag cggcccggcg gccggtgctg
     2941 cctcgggcgg cggcggtgtc ctcgccggca cctccggatc ggcgatcgtc cgctggacgc
     3001 tggtcatcga gcccggtggg cgagccggga tccactggga ggtcacgacc gacctcgagc
     3061 cgatgcccgg cgcggtaccc gccgaggcga cgccgacgcg tcttgcgcga tccgtgacga
     3121 gcggccagcc ggacgacggg agcgcggcag tcgcggagcc gaccgtcgcg gaggccgagt
     3181 acggcgcctg ggacgagcgc tgcgcccgga tccggtcgga tggggagctg ctggaccttg
     3241 cgatccggcg aagcattgcc gaccttcgcc tgctgcggaa tgacgggccg ttccagggtg
     3301 agcactacgt cgcggccggc atcccgtggt tcgcgaccct gttcggtcgc gacagcatca
     3361 tcacctcgct ccaggtcgtg ccgttcatgc cggatgtcgc gcgcgagacg ctccgcgtgc
     3421 tcgcggactg gcaggcgacc gaggacgacc cggaacggga catggagccg ggcaagatcc
     3481 tgcacgagct tcgcgtgggc gagctcgcgc gcacgggcga gctcccgcac cgaccgtatt
     3541 tcggcagcat cgattcgacg ccgctgtggc tggtcctgct cgacgagacg taccgctgga
     3601 ccggcgacct cgagctcgtg cgctcgctgt ggccgaacgc gctggcagcc ctcgagtgga
     3661 tcgacaagta cggcgatcgc gacggcgacg gcttcgtcga gtacgaacgg cggaccccgc
     3721 acggcctcct gaaccagggc tggaaggact cgggcgacgc gatccgccat cgcgacggca
     3781 gcgtcgccgt ggcgccgatc gcactggtcg agatccaggg ctacgtgtat gacgcgaagc
     3841 gccgcatggc ctcgctcgcc gagcgcctcg gcgacggcga gctcgcggcc cggctgaacg
     3901 aggaggcaag cacgctcgcg cggcgtttcg acgaggcgtt ctggatgccc gacgtcggct
     3961 actacgcgat cgcgctggac cgcgacaagc accaggtcgg gacgatcagc tcgaacccgg
     4021 gccattgcct gtggagcggc atcgttccgg cggagcgtgc cgacgcggtc gtgaatcacc
     4081 tcctggatcc gtcgatggac tgcggctggg ggattcgcac ctacgcagcc ggccagcccg
     4141 ggtacaaccc ggtcggctat cacacgggct cggtctggcc gcacgacaac gcgctgatcg
     4201 cggcgggcat gaagcgcgtc ggccggcacg acgccgcgga ccg
//
LOCUS       DATBSW010000161        18366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_367207, whole genome shotgun sequence.
ACCESSION   DATBSW010000161 DATBSW010000000
VERSION     DATBSW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 18366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18366
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367207"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1483)
                     /locus_tag="VIF63_04450"
     CDS             complement(164..1483)
                     /locus_tag="VIF63_04450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8238666.1"
                     /translation="MPTVSDIVLAAIGILATVAFALLLAPTFVWVRRRLSSGEEETET
                     KAEGMLDSKAIEKELAGRRGVAAGADEPEDIAPAIGPTSPDPSLEDRIIRIVSWAFLM
                     AVAVFAAASGLWDATLSAIVILIAVTGEVLLILQDVLPRTPLRQARGPLQGALALAFA
                     GALVALTGGFTSPFTFIFPLIVGAGALLVAPRVAFILAITATAAYLTAGLVDNRNPAT
                     GPLVQMAVNLTGLYLLAYIGASIGREQRRARDAAIRMSTVDALTGLYNRSFFFTALER
                     EIARGDRSGRAFCLVMLDLDDLKSVNDRFGHLAGDQVLRSISDIVKGGVRKIDVAARY
                     GGDEFVALLPETDPTGGWVLAEKIRQTVAEQGMPGIDPVPTVSVGVVSYPADGRSADA
                     LLVSADRAMYASKRGGKNRVARATAEPMVVSIEAHEAVRNREGERAG"
     gene            complement(1483..1977)
                     /locus_tag="VIF63_04455"
     CDS             complement(1483..1977)
                     /locus_tag="VIF63_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY8238667.1"
                     /translation="MRDSNPDDATRRNRRPNELSPLDKRIIEHLQQDGRRPFTQIAAD
                     LGVSEAAVRARTNRLVERGILQIVGVADPDALGFQQALIGVRCQPGKLLEVAEALASM
                     PEVDYVVVTTGRYDVLIETVAEDNEGLLHFLADRLQRIDGVRDTETFSYLRLVKQTYQ
                     FGTR"
     gene            complement(2032..2961)
                     /locus_tag="VIF63_04460"
     CDS             complement(2032..2961)
                     /locus_tag="VIF63_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8238668.1"
                     /translation="MSPTARPLRVGIQLPEVERIVRWPEYLAMAKAAEDCGFDTIWLG
                     DHLLYRYEDGSTRGPWEVWTTLSAIAASTSRIKLGPLVAATAFHSPAMLAKLAATVDE
                     VSGGRLILGLGAGWNDTEFRAFGFPFDHRISRFEEAFTIIRTLLREGSIDFDGTFFQA
                     RDMELLPPPTRPGGPPLMVGSIGPRMLEITLPYVEQWNVWYRQTNNSPEGLEPILRQV
                     DEACRAVGRDPATLEKTSAVLVGMPGAAGRVTKYDDNEVAPRALEGSSSAIADGLRAY
                     AALGLAEVQLIVDPITVESIEGLAPVLVDLDRG"
     gene            complement(2994..3455)
                     /locus_tag="VIF63_04465"
     CDS             complement(2994..3455)
                     /locus_tag="VIF63_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238669.1"
                     /translation="MTEFADNAPQLEHAAALRRTSDEFLRRLDRLLELETHKREMAPD
                     APEFVRLAREVEDLSRALLFTGGQQVELAEEVHYEAKRNDVAIEQSIRDTPPKRAAVS
                     ILDEWRNAERRLAAAALGSEEEASARADVERLREEYRRLTAPIPPEPGGRA"
     gene            3597..4709
                     /locus_tag="VIF63_04470"
     CDS             3597..4709
                     /locus_tag="VIF63_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEY8238670.1"
                     /translation="MATISEDRTATWPAPSVAPTDGPLTFEELQLAGRNRGMPLEALR
                     YDVTPTGLHYLLIHFDIPATDAATWRLRLDGLVERPLELSLADLRARERRTVPVTLEC
                     AGNGRARMRPRPLSNPWLFEAIGTAEWGGTPLAPLLEEAGVLPEAVELVFTGADRGVQ
                     GGVVHDYQRSLQLSEARRPEILLVDEMNGEPLQPQHGFPLRLLVPGWYGMTSVKWLHR
                     IEAVATPFDGYQQAAAYHFRDSADDPGVPVSRIRVRALMAPPGIPDYFTRKRLVDAGP
                     VRISGRAWSGSAPVTKVEVGVDGNWTEARLAEPVGEWAWRGWSFDWDARPGERTLACR
                     ATDATGDVQPLESPWNYQGMGNNGVQAIQVTVRDRG"
     gene            4775..5722
                     /locus_tag="VIF63_04475"
     CDS             4775..5722
                     /locus_tag="VIF63_04475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8238671.1"
                     /translation="MRRLGLGAAASVLRAIRPGPATEARLLRWSWASRPAQGLDEYLV
                     IGWQNPRINVQSILIRHELLRRVFGDVDADLMRAELAFAVELNEALRLESKRTNTPIN
                     SFLDQRRMARIREVGAVIADRETEYRDRWRELLAGRSAPRVSVLELACGSANDYRAFA
                     DYGLAPFLDYAGIDLNPKNIINARRRFPDVEFRQGSALDLDDADASVDVVLASDLLEH
                     LPLEGADRVIAEASRVARRAVILSFFNMSEIPEHLDRPFAAYHWNQLSRAKVEAALSA
                     RFPRVTVIPVHAWLKDEFGYGHTYNKRAWTLIAERDAPA"
     gene            complement(5728..5804)
                     /locus_tag="VIF63_04480"
     tRNA            complement(5728..5804)
                     /locus_tag="VIF63_04480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5768..5770),aa:Pro,seq:tgg)
     gene            5958..7100
                     /locus_tag="VIF63_04485"
     CDS             5958..7100
                     /locus_tag="VIF63_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238672.1"
                     /translation="MEPSGVTLSVRHLRAPAVVVALIVVLAAACQGPAPTPSAAPAAT
                     TPPARATAPAPAATAAPSAGLPGPIKVVGTAPVITRSMFPGRTATLPGAISVAADGTY
                     HAWVIAFAQTPGTQEIHYLTSPDALTWTEVPDASLEGLSEGLGNPGALPTSVLEDGDG
                     WVMYYVGTLATEQRGWDLWRATAPTAAGPWTRRDEPVMRRGPADAWDSGALDFPTVLA
                     TDDGYTMVYSGWNPAVPDGGSIGFATSDDGIAWTKLDDPATTAAPFAESDPIVEPGLC
                     GDFDDRAVQQPRVVALPDRLVMAYAGYTGPLESRATVGYADSLDGGRTWTCAWPGPAL
                     DTAGLPDGFVHTLTAFHHGERAALLVEWFANGGTDIWLADLGVSPP"
     gene            7307..8620
                     /locus_tag="VIF63_04490"
     CDS             7307..8620
                     /locus_tag="VIF63_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8238673.1"
                     /translation="MAAAASTFVPGLGQLLNGRARLAKWFALPILLLAIVGFVAVSGR
                     SPSRLVASIVAPGILSVLLALNAAVLVWRLVAVGHAFFDRRYSGRPGALATIGIVVLL
                     AAVAAPHLVLNSWGSTAQTSFANVFTNDDASKDPLAAPVDRGPGIDERLNLLLVAIDK
                     TPDRPETLTDSMMVVSVDPIGNSVTMLSLPRDMVGVPLGNGEAFLPKLNSLMTYAEEH
                     PQKFPDGGMRALQDAVGAMLGIRIHNYVKVDFFGFVKLVDAIGGVDVDVTRAFYDPLY
                     DGLGVNPKGVRGWGADVGLHHFNGWEAIAYSRTRRGVGESDFTRAARQQEILLAIRDK
                     LVSDPGLMLNVPALFDAFDDLVQTDVPPALLPGLAALAEEVETSNIVRVVLRRPLVKG
                     GTDPVYGSVQRPSFAAILKVAATLFPPAGIAPIPWPTPASSQKPS"
     gene            complement(8605..9258)
                     /locus_tag="VIF63_04495"
     CDS             complement(8605..9258)
                     /locus_tag="VIF63_04495"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY8238674.1"
                     /translation="MTGPDRKSSARSRLVVATRSEHKLRELRDMLRLGRANLVSLDEL
                     AVVGEPVEDGLTFETNARIKARFAARATGLPALADDSGLEVDALGGGPGVRTRRYAGP
                     DASDAENNAKLLLELAGVPLAQRGARYVCVLVLALPDADSPGGLRLISRRGTSRGRIA
                     LEPRGTGGFGYDPLFEPLRERAGGRTFGEYDRAEKDAISHRGRAARRMTGVLSQLGF"
     gene            complement(9255..9992)
                     /gene="rph"
                     /locus_tag="VIF63_04500"
     CDS             complement(9255..9992)
                     /gene="rph"
                     /locus_tag="VIF63_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8238675.1"
                     /translation="MSIGNRPPSVRDDGRRPGDLRPVTMTLGVLKWAEGSCRIRAGDT
                     EVLCAATVGDRVPPHLRGKGTGWVTAEYSMLPRATAERSDRESVKGRIGGRTHEIQRL
                     IGRSLRGVVDLGKLGEKTITVDCDVLQADGGTRCASITGGYVALAAALIAAGLERTLV
                     GKVAAISVGVVNGLTMLDLDYSEDSRAEVDMNVVGTDAGTYIEIQGTAEGLPFDRARV
                     GSLLDLADTGLARLFEQQAAALATVRR"
     gene            complement(9989..11284)
                     /gene="mazG"
                     /locus_tag="VIF63_04505"
     CDS             complement(9989..11284)
                     /gene="mazG"
                     /locus_tag="VIF63_04505"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY8238676.1"
                     /translation="MLDDLVAEARARWGLDVSAGIQIVPADGLTAAPIEASRPLLIVP
                     LAVLGGATDGTASDGAAGDGAARPLPGRHGPAGRDPLAVLARLYPADATVARLGAPVD
                     GDTSGAPSGGATTIGAATTIGALTARDLEHPLYVRPIAPELASAGPWAMPYISDRLRQ
                     PDGCPWDREQTHASLRKHLLEETYEVYDALEHGATPALAEELGDLLLQVILHAQLAAE
                     AGVFDLADVQASIARKIVRRHPHVFGDAVARTSADVNRQWERIKAGEREAAAEAAEAA
                     GSGGDGSAPVPKSALDGISPSMPALAASQEMQERASALGYDWPSIDGVLDKVAEELGE
                     LASAATSPEQQEEFGDLLLVVVNVGRKLGIESEAALRAANHKFRTRFGRVERMARERG
                     VALRDLDFAALDQLWDAAKAEERAAAADASVAVGSEGVR"
     gene            complement(11293..13026)
                     /locus_tag="VIF63_04510"
     CDS             complement(11293..13026)
                     /locus_tag="VIF63_04510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8238677.1"
                     /translation="MTLRARPVARRRGRAGWDSGDRRNSLINLGFFIAIGLAVLILVG
                     YTAWSWYDDHFGAAATVNGKVITKDDLRGRLVIENFRLDYIESRIQTLMAKGQISPSD
                     GQQQIQFLAQRRQQLANLTLERLVDNALMGTLADQNGITVSDADIDDQLHQEATTSEQ
                     RHVYMIEIEPSADPITGEVDEAQKRAALDRAQRAIARLKAGESWEDVARTASDSGLAP
                     QAGNLGWLSKDSGYDEAFMAAVFAAPVNTPTEIVEGEDGAYRIGRYTESTPEELDPTF
                     QSRIEKDGILLADYRTAARNDVIRKKLSDKIVADLSKPSKQRHVLEIYLPEPNTSSTP
                     GDVGAKVRWIVFSPKDDIANADELPLTDPAWVEAKAEADAAYSALRADISRFDAMART
                     DSDEGSAVGTGGKQPWIYPTSAIDEVIRSIVLEPTRKPDELIAPVKGELGWYVIQYLR
                     PSGDGETAYLNQLKADVGDDASFMRVAKNTSQGSEARDGGDLGWIATGQLADELDKAV
                     FSTNVGSLSSVVTIPSDGDYLLRVIGEETREATAEQLKIFKDSGFSFWYTRQKEAADI
                     VYNLGSSSGTA"
     gene            complement(13105..14220)
                     /locus_tag="VIF63_04515"
     CDS             complement(13105..14220)
                     /locus_tag="VIF63_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEY8238678.1"
                     /translation="MRTTFAMARVMPLRRRVVFGTSAAADLTNNLAFIRAELDRRGIP
                     YSVVARLPAITWGTWFGSFLANIRAAFHLATARLFIVDDYYFPVYVIEPRKGTTIVQT
                     WHASGAFKKIGYSVLDKTFGADEGLIAKVDIHSNYDICLMASKTAAVHYAEAFRTPLE
                     RFETDVGMPRTDVLFGDAVPGIKASIREHYHLPEGKRVILYAPTFRGATTRKATSPTD
                     LDFAELHRVLGEDHIVLLRLHPFVRQAHPIPAELASFAIDVSDHPDIHELMLVSDVLV
                     TDYSSAIFEFSLLTRPMAFFAPDHDDYMDERGFYFDYRTGVPGPVFETTADLAAWLRA
                     GAFDLDRIARFRDESFEVADGHATERFVDRIVVPALA"
     gene            14471..15157
                     /locus_tag="VIF63_04520"
     CDS             14471..15157
                     /locus_tag="VIF63_04520"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HEY8238679.1"
                     /translation="MTFGMVVLAGGIGRRIGRPFPKQFLLLGGKPLIIHVLEKARAIP
                     ELERIVITCPEAHLDETVQLLKNHRMDRRFSCIVGGATRQESVYKGLQALDGVDSVVI
                     HEAVRPLVTTEEFRALIASPDENVMFGIPISFTVLRGHEYVEGLLDREELVNVQLPQK
                     FDRAKLLAAHEASRAAGTSFTEDASLFVEHSGEKVRILGGSERNVKITVPADIVMAEA
                     LYADSIGRGS"
     gene            15161..15865
                     /locus_tag="VIF63_04525"
     CDS             15161..15865
                     /locus_tag="VIF63_04525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8238680.1"
                     /translation="MGRTALVTGASQGIGRATAVRLSRDPSITKLVLVARSADGLAAT
                     AEACAADADKLDLVAYDLADLDGIPDLVAEIHERHGGIDVLLNIAGYAEPKSLLDTTA
                     KNLVTTFTVNVFAMLILTREVVRNMQGRPTKIVNIASTAGITARPGWVAYASSKAAVV
                     SLSQTLSEELAGTGVKIYTISPGRTATELRRKLAPEEDPSTIMQPDAVADVIAMLVGE
                     GERTLDGQNIIVRQQI"
     gene            15894..17009
                     /locus_tag="VIF63_04530"
     CDS             15894..17009
                     /locus_tag="VIF63_04530"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEY8238681.1"
                     /translation="MNRLEHTLASAVLRVLGFFFDRLPIRHRVVLTTARVAHLEGNLA
                     YLHAAIRRARPDVPLVTLLEPYSYGLAGKLAYLLRLVRGTYLLRTSSLFVVDNAYLPV
                     HVAPHRRGTTVVQVWHGVGALKRFGADTAKGLDEPERSFLHHHYDFVVCAGEASRAPY
                     AAALRTPIERVQPLGAARTDFFFDPAAIVAARETTLATYPALRAGRVVLYAPTFRGRG
                     RGKRAAPGFDAAAVRGALPPGHVLALKSHPNLDPALVAAAGFDVVIEPSVELNQVLAA
                     TDVLVTDYSSAIYEWALLRRPLVLLTEDLEAYERDPGMYLDARTELIGERIDDPADLA
                     PAILRATVDDAAWTAFIARRIEACDGGASERFVERFL"
     gene            17006..>18366
                     /locus_tag="VIF63_04535"
     CDS             17006..>18366
                     /locus_tag="VIF63_04535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8238682.1"
                     /translation="MTGPGKRPRFSIVTAVYDVEPYLPDFIASIEAQRVPAADLEVVA
                     VDDGSTDGSLDLLLDWARRSRLRVKVFTKPNGGQGSARNLGLTHATGEWVNFADPDDM
                     LDPDFLRVAARFAAANPEVEIMAGKPISLIESVGRIADNHPRRHQYRRGNRVANLLDE
                     PNVFSGSATVSLFRLDRVRGMDLRFDPRIRPNFEDGHFAIHYLLALDVPRVGLLRDAR
                     YLYRRRAAGTSTLQGSLAHPGRFTDVLEHGYNEVLDAATALHGSIPAWLQQVIVYELS
                     WYLSADEKITSDIVLADELVPRFHELMARIVSRLEPDVVAAHRVRKLRVVWADILAHG
                     YREETWHMPFVVRGKLDREMGLRRLGYRFVGAQPDERFVVDGVEVAPAFAKTRAHRYY
                     GRVLIDERIIWIPWGTTIQVFLDGVETMTRHRWAPRFGRRGRRALAERLPIYRRLPLP
                     FFVA"
BASE COUNT         2616 a         6509 c         6487 g         2754 t
ORIGIN      
        1 gctcgggccg agcggctgcg ggccggtcag accccggccg gcacggcagc ggccgcccgc
       61 gccgcggcgc ggatcgcgcg ggtggagaag cctcgcctgt cgtcgctcat cgccggcgat
      121 gctacaccgc gcagggctcc cggccggcgg gagccgctcc ggttcagccg gcgcgctcgc
      181 cctcgcggtt gcgcacggcc tcgtgggcct cgatcgacac caccatcggc tccgccgtgg
      241 cgcgcgcgac ccggttcttg ccgccgcgct tcgatgcata catcgcccgg tccgcgctca
      301 cgagcagcgc gtccgcggag cgcccatcgg cggggtacga gaccaccccg accgagacgg
      361 tcgggaccgg gtcgatgccg ggcatgccct gctccgcgac ggtctggcgg atcttctcgg
      421 ccagcaccca gccgcccgtt ggatcggtct cgggcaggag cgccacgaac tcgtcgcctc
      481 cgtagcgcgc tgcaacgtcg atcttgcgga cgccgccctt gacgatgtcc gagatgctgc
      541 gcaggacctg gtcgccggcg aggtgcccga accggtcgtt gaccgatttg aggtcgtcga
      601 ggtcgagcat caccaggcag aaggcgcggc cggatcggtc gccgcgggcg atctcgcgct
      661 cgagcgcggt gaagaaaaat gaccggttgt acaggccggt cagcgcgtcg acggtggaca
      721 tgcggatcgc cgcatcacgt gcccggcgct gctcacgccc gatggatgcg ccgatgtagg
      781 cgagcaggta caggccggtc aggttgacgg ccatctgcac cagcgggccg gtcgcggggt
      841 tccggttgtc gacgagccct gcggtcaggt acgcggcggt ggccgtgatg gccaggatga
      901 aggcgacacg cggggcgacc agcagggcgc ccgcgccgac gatgagcggg aagatgaacg
      961 tgaacgggct cgtgaacccg ccggtcaatg cgacgagcgc gcccgcgaac gcgagggcga
     1021 gcgcgccctg gagcggcccg cgcgcctggc gcaggggcgt ccgcggcagc acgtcctgga
     1081 ggatcagcag gacctcgccg gtgacggcga tcaggatgac gatcgcggac agcgtcgcgt
     1141 cccacagccc gctcgcggcg gcgaacaccg ccaccgccat gaggaacgcc cagctgacga
     1201 tccggatgat ccggtcctcg agcgacgggt cgggcgaggt cggaccgatc gcgggggcaa
     1261 tgtcctcggg ctcgtcggcg cccgctgcga ccccgcggcg ccccgcgagc tccttttcga
     1321 ttgccttgga gtccagcatg ccctccgcct tcgtctctgt ctcttcttcg ccgctggaca
     1381 accgccgtcg cacccacacg aacgtcggcg cgaggagcaa cgcgaacgcc acggtcgcga
     1441 ggatcccgat cgcggcgaga acgatgtcgg aaacggtggg catcagcgtg tcccgaactg
     1501 gtacgtctgc ttcacgagtc ggaggtacga gaacgtctcg gtatcgcgca cgccatcgat
     1561 ccgctgcaag cggtccgcga ggaagtgcag gagcccttcg ttgtcctcgg cgacggtctc
     1621 gatcaggacg tcgtagcgcc ctgtcgtaac caccacgtaa tcgacctcgg gcatgctcgc
     1681 gagtgcctcc gcaacctcga gcagcttgcc cggctgacag cgaacgccaa tgagcgcctg
     1741 ctggaaaccg agcgcatccg ggtcggccac gccgacgatc tgcaggatgc cacgctcaac
     1801 gagcctgttg gtgcgggcac gcaccgctgc ttcagaaaca ccgaggtcgg cggcgatctg
     1861 ggtgaagggc cggcggccgt cctgctggag gtgctcgatg atccgcttgt caagcggaga
     1921 cagctcgttg gggcggcgat tccggcgggt cgcgtcgtcc ggattcgaat ctcgcactcc
     1981 tgccccttcc ttgcggccga tgctagcacc ggctccgtgg cccttccggg cctagccgcg
     2041 atcgaggtcc accagcacgg gcgcgaggcc ctcgatggac tcgacggtga tcggatcgac
     2101 gatcagctgg acctccgcga ggcccagcgc cgcataggcc cggaggccat cggcgatggc
     2161 cgacgacgag ccctcgaggg cccgcggcgc gacctcgttg tcgtcgtatt tcgtgacgcg
     2221 tccggcggcg ccgggcatgc cgacgagcac cgcgctcgtc ttctccaggg tggcggggtc
     2281 gcggccgacg gcgcggcagg cctcgtcgac ctggcggagg atcggctcga ggccctcggg
     2341 cgaattgttc gtctggcggt accagacgtt ccactgctcg acgtacggca gggtgatctc
     2401 gagcatccgc gggccgatgc tgccgaccat cagcggcggc ccacccggtc gcgtcggtgg
     2461 cgggagcagc tccatgtcgc gggcctggaa gaacgtgccg tcgaagtcga tcgagccctc
     2521 gcgcaggagc gtccggatga tcgtgaacgc ctcctcgaac cgcgagatcc gatggtcgaa
     2581 cgggaagccg aaggcgcgga actccgtgtc gttccagccg gcgccgaggc cgaggatcag
     2641 gcggccgccg ctgacctcat cgaccgtcgc ggcgagcttc gcaagcatgg ccggggagtg
     2701 gaacgccgtc gcggcgacga gcgggccgag cttgattcgc gaggtgctcg cggcaatggc
     2761 cgagagggtc gtccagacct cccacggccc gcgggtcgag ccgtcctcgt agcggtacag
     2821 gaggtgatcg cccagccaga tcgtgtcgaa gccgcagtcc tcggcggcct tcgccatcgc
     2881 caggtactcg ggccagcgca cgatgcgttc gacctcgggc agctggatgc cgacgcgaag
     2941 cgggcgggca gtcgggctca cccgcaaagc ctatcgagca atgccgaggg ccgtcaggcc
     3001 cgccccccgg gctcgggcgg aatcggggcg gtcaggcgcc gatactcctc gcggagccgc
     3061 tcgacgtcgg cgcgtgccga cgcctcctcc tcggagccga gggcagcggc cgcgaggcgc
     3121 cgctcggcat ttcgccactc atccaggatc gacacggctg cgcgcttggg cggcgtatcc
     3181 cggatcgact gctcgatcgc gacgtcgttg cgcttcgcct cgtagtggac ctcttcggcg
     3241 agctcgacct gctggccgcc ggtgaacagc agcgcccgag acagatcctc gacctcgcgg
     3301 gccaggcgga cgaactcggg cgcgtccggc gccatctcgc gtttgtgggt ctcgagctcg
     3361 aggagccggt cgagccgccg caggaactcg tcggaggtcc gccgcagggc ggcggcatgc
     3421 tccaactggg gtgcgttgtc ggcgaattca gtcatgggtt gtcctccccg ccgcgaggac
     3481 ccgcggctcg cgcgatgctg cgcgatgcgg cagttcgggg agtccctcgc ccggcgccca
     3541 agaggtgaca gccggctatc ggaagttcgg atcgcgcccg gcgtaccatc gggccgatgg
     3601 cgacgatctc cgaggaccgc acggccacgt ggcctgcgcc ctcggtcgct ccgaccgacg
     3661 gcccgctcac cttcgaggag ctccagctcg cgggccgcaa ccggggcatg ccgctcgaag
     3721 cgttgcgcta cgacgtcacc ccgaccggcc tccactacct gctgatccat ttcgacatcc
     3781 ccgcgacgga cgcggccacg tggcggctcc gcctcgacgg cctggtcgaa cggccgctgg
     3841 agctgtccct ggcggacctg cgtgcgcggg agagacggac agtgccggtc accctcgaat
     3901 gcgcgggcaa cggccgggcg cgaatgcggc cgcggccgct cagcaacccg tggctgttcg
     3961 aggcgatcgg gaccgcggag tggggcggga cgccgctcgc gccgttgctc gaggaggccg
     4021 gggtgctgcc cgaggccgtg gagctcgtgt tcaccggcgc ggatcgcggt gtccagggcg
     4081 gggtcgtgca tgactaccag cgcagcctcc agctctcgga ggcgcggcgc ccggagatcc
     4141 tgctcgtcga cgagatgaac ggcgagccgc tgcagcctca gcacggcttc ccgctgcggc
     4201 tgctcgtgcc cggctggtac ggcatgacca gcgtgaagtg gctccaccgg atcgaggcgg
     4261 tcgccacgcc gttcgacggc taccagcagg cggcggcgta tcacttccgc gacagcgcgg
     4321 acgatcccgg cgtgcccgtc tcgcgcatcc gcgtccgggc actgatggcg ccgccgggca
     4381 tccccgacta cttcacccgg aagcggctgg tcgacgccgg cccggtcagg atcagcgggc
     4441 gggcgtggag cgggtcggca ccggtcacga aggtcgaggt tggcgtcgac ggcaattgga
     4501 ccgaggcacg cctcgccgag ccggtcggcg aatgggcgtg gcgcggctgg tcgttcgact
     4561 gggacgcgcg acccggcgag cgcacgctgg cctgccgcgc gaccgacgcc accggcgacg
     4621 tgcagccact cgagtcgccc tggaactacc agggcatggg caacaacggc gtccaggcga
     4681 tccaggtcac cgtccgagac cggggctagc ccaggtggac tccggccgcc tcgcctcggg
     4741 tctgcgtcgg cacgggatgc gggcgaagcg gcgcgtgcgg cggctcggcc tcggcgcggc
     4801 agcgagcgtc ctgcgagcca tccggccggg accggcgacg gaggcccgcc tgctgcgctg
     4861 gagctgggcg tcgcgccccg cgcagggcct cgacgagtac ctcgtgatcg gctggcagaa
     4921 cccccggatc aacgtccaga gcatcctcat ccgtcacgag ctgctccggc gcgtgttcgg
     4981 tgacgtcgat gccgacctga tgcgggccga gctcgcgttc gcggtcgagc tcaacgaggc
     5041 gctgcggctc gaatcgaagc gcacgaacac gccgatcaac tcgttcctcg accagcgccg
     5101 aatggcccgg atccgcgagg tcggcgcggt gatcgccgat cgcgagaccg agtaccgcga
     5161 ccgctggcgc gagctgctcg cgggccgaag cgcgcctcgg gtttccgtgc tggagctcgc
     5221 ctgcggctcc gcgaacgact accgcgcgtt cgccgactat ggcctcgcgc cgttcctcga
     5281 ctacgccggc atcgacctga acccgaagaa catcatcaac gcccgccggc ggttcccgga
     5341 cgtcgagttc cggcagggca gcgcgctcga tctcgacgac gcggacgcgt cggtcgacgt
     5401 cgtgctggcg tccgacctgc tcgagcacct gccgctcgag ggcgccgatc gcgtcatcgc
     5461 cgaggcatca cgcgtcgccc gacgcgcggt gatcctgtcg ttcttcaaca tgtccgagat
     5521 cccggagcac ctcgaccgtc cgttcgcggc gtatcactgg aaccaactga gccgtgccaa
     5581 ggtcgaggcg gcgctgtcgg cgcgattccc gcgcgtgacg gtgatcccgg tccacgcctg
     5641 gctcaaggac gagttcgggt acggccacac gtacaacaag cgtgcctgga cgttgatcgc
     5701 ggagcgcgac gcgccggcct gaggacgtgg tcggggcggt cggattcgaa ccgacggcct
     5761 catgctccca aagcatgtgc gctaccaggc tgcgctacgc cccgtggacc gccgccattc
     5821 taggcgttgc cctagaagag cccgccgcgg tcacagcggc ccgcgttcgg ggctctgtgg
     5881 gctagccgag attcgtggga accactcacg gaggagcggc gtctcaggcc cgacgccggg
     5941 cgggagctgg caggctgatg gaaccgtccg gcgtcaccct ttccgttcga catcttcgag
     6001 cgccggccgt cgtcgtcgcg ctcatcgtgg tgctcgctgc ggcctgccag gggccggcgc
     6061 cgacaccttc ggcggcacct gcggccacca ctcccccagc gagggccacg gccccagcgc
     6121 cggctgcaac cgcggcgccg agcgccggcc tgccgggccc gatcaaggtc gtcgggacgg
     6181 cgccggtgat cacccgaagc atgttccccg gccgcaccgc gacgttgccc ggcgcgatca
     6241 gcgtcgccgc cgatggcacg taccacgcgt gggtgatcgc cttcgcgcag acgcccggca
     6301 cgcaggagat ccactacctg acctcgcccg acgcgctgac ctggaccgag gtcccggacg
     6361 cgtccctcga aggcctgtcc gaggggctcg gcaaccccgg cgcgctgccg acgagcgtcc
     6421 tcgaggatgg cgacggctgg gtcatgtact acgtcgggac gctcgcgacc gagcaacgag
     6481 gctgggatct ctggcgggcg accgcaccga ccgcggccgg gccgtggacc aggcgtgacg
     6541 aaccggtgat gcgacgcggc ccggcggacg catgggattc gggcgcactc gacttcccga
     6601 ccgtcctcgc caccgatgac ggctacacga tggtctattc cggctggaac cccgcggtcc
     6661 ccgacggcgg ctcgattggc ttcgcgacgt ccgacgacgg catcgcgtgg acgaagctcg
     6721 acgacccggc gacgaccgca gcgccctttg ccgagagcga cccgatcgtc gagccggggc
     6781 tgtgcggcga cttcgacgac cgcgcggtcc agcagccgcg cgtcgtggcc ctaccggatc
     6841 gactggtgat ggcgtacgcc ggctacaccg gcccgctcga atcgcgagcg acggttgggt
     6901 acgcggacag cctcgacggc ggccggacct ggacgtgcgc gtggccgggc ccggcactcg
     6961 acacggccgg cctgcccgac ggcttcgtgc acacgctcac cgcgttccac cacggcgaac
     7021 gggccgcgct gctcgtcgag tggttcgcga acggcggcac cgacatctgg ctcgcggatc
     7081 tcggcgtcag cccgccctga ggcgcgtcgc ggtctcgggc acggccgctg gtaaactgcc
     7141 gacggacggt gtgcccaacc ggcccgacga cccgaactcg caacaggagc cccgtccgca
     7201 cgtgcaaggt actcgggcgc ccacgcgccg cggacacgcc cggccggtca cggtacgacc
     7261 ggagcggcaa cccggcgtcg attctcgtcc ggatcttcgc cgcaccatgg cggcagccgc
     7321 ctcgacgttc gtcccgggac tcgggcagct gctgaatggg cgcgcgcggc tggccaaatg
     7381 gttcgccctg ccgatcctgc tgctcgccat cgtgggcttc gtggccgtct cggggcgctc
     7441 gccatctcgc ctcgtggcct cgatcgtcgc tccgggcatc ctgtcggtcc tgctggcgct
     7501 caacgccgcc gtcctcgtct ggcgcctggt cgccgtcggg cacgcgttct tcgatcggcg
     7561 ctactcggga cggcccggcg cccttgcgac gatcggaatc gtcgtcctgc tggcggcggt
     7621 cgccgcgccc catctcgtgc tcaattcgtg gggctccacc gcgcagacgt cgtttgcgaa
     7681 cgtgttcacc aacgacgacg catcgaagga cccgttggcc gccccggttg atcgtgggcc
     7741 cgggatcgac gagcggctga accttctcct cgtcgccatc gacaagacac cggaccggcc
     7801 cgagaccctg accgattcga tgatggtcgt gtcggtcgac ccgatcggca attcggtgac
     7861 catgctctcg ctcccgaggg acatggtcgg cgtgccgctc ggcaacggcg aggcgttcct
     7921 gccgaagctg aactccctca tgacctacgc cgaggagcat ccccagaaat tccccgacgg
     7981 cggcatgcgc gccctccagg acgcggttgg tgcgatgctc ggcatccgca ttcacaacta
     8041 cgtgaaggtc gacttcttcg ggttcgtgaa gctcgtggat gcgatcggcg gcgtcgacgt
     8101 cgatgtcacc cgggcgttct acgacccgct ctacgacggg ctcggcgtga accccaaggg
     8161 cgtccgcggc tggggcgcgg acgtcggcct ccaccacttc aacggctggg aggcgatcgc
     8221 ctacagccga acgcggcggg gcgtgggcga gagcgacttc acgcgggccg cgcgccagca
     8281 ggagatcctg ctcgcgattc gcgacaagct cgtcagcgac ccggggctga tgctgaacgt
     8341 accggcactg ttcgacgcgt tcgacgatct ggtccagacc gacgtgcctc cggccctgct
     8401 cccgggtctc gcggcgctcg ccgaggaggt cgagacatcg aacatcgttc gggtcgtcct
     8461 caggcggccg ctcgtgaagg gcggcacgga cccggtctac ggctcggtcc agaggcccag
     8521 cttcgcggcg atcctgaagg tcgcggcgac gctctttccc ccggctggca tcgccccgat
     8581 tccgtggccc acccccgcga gcagtcagaa gccgagctga ctgagtacgc cggtcatccg
     8641 ccgagccgca cgaccgcggt gcgagatcgc gtccttctcg gctcggtcgt actcgccgaa
     8701 ggtccggccg ccggcgcgct cgcgcagcgg ctcgaacagc gggtcgtagc cgaagccgcc
     8761 ggtcccgcgg ggctcgaggg caatccggcc gcggctcgtc ccgcgacggc tgatcaggcg
     8821 caggccaccg ggcgaatccg cgtcgggtag cgcgagcacc aggacacaga cgtagcgggc
     8881 gccgcgctgc gcgagcggca cgccggcgag ctccagaagc agcttcgcgt tgttctcggc
     8941 gtccgacgcg tccgggccgg cgtagcgccg ggtgcgaacc ccgggtccgc cgccgagcgc
     9001 gtcgacctcg agaccggagt catcggcgag ggccggcaag cccgtcgccc gagccgcgaa
     9061 tcgcgccttg atccgcgcgt tggtctcgaa cgtgaggccg tcctcgaccg gctcgcccac
     9121 caccgcgagc tcgtcgaggg agacgagatt cgcacgcccg agccggagca tgtcgcgtag
     9181 ctcgcgcagc ttgtgctccg agcgtgtggc aacgacgagg cgcgagcgag cgctcgactt
     9241 gcgatcggga ccggtcagcg acgcaccgtc gcgagcgcgg ccgcctgctg ctcgaagagc
     9301 cgcgccaggc cggtgtcggc gaggtcgagc agcgagccga cgcgggcccg gtcgaacggc
     9361 aggccctcgg ccgtgccctg gatctcgatg tacgtcccgg cgtccgtgcc gacgacgttc
     9421 atgtcgacct cggcgcggga gtcctcggag tagtcgaggt cgagcatggt cagcccgttg
     9481 acgacgccga cggagatcgc cgcgaccttg ccaacgagcg tgcgctcgag gcccgcggcg
     9541 atcagcgccg cagcgagggc gacgtagccg ccggtgatcg atgcgcagcg cgtgccgcca
     9601 tcggcctgca ggacgtcgca gtcgaccgtg atcgttttct cgccgagctt gccgagatcg
     9661 accacgccac gcagcgagcg gccgatgagc cgctggatct cgtgcgtccg gccgccgatc
     9721 cggcccttca ctgactcgcg atccgagcgc tccgcggtag ccctcggcag catcgaatac
     9781 tcagcggtga cccagccggt gcccttgccg cggagatggg gcgggacgcg gtcgccgacc
     9841 gtggccgcgc acaggacctc ggtgtcgccg gcccggatgc ggcacgagcc ctcggcccac
     9901 ttcaggaccc cgagggtcat cgtgaccggg cgcaggtcac cggggcgcct gccgtcgtcg
     9961 cggaccgacg gcggccgatt gccgatgctc atcgcacgcc ctcactcccg actgcgaccg
    10021 acgcgtccgc tgccgcggcc cgttcctcgg ccttggcagc atcccacagc tggtcgaggg
    10081 ccgcgaaatc gaggtcgcgc agggcgacgc cccgttcgcg ggccatgcgc tcgacgcgcc
    10141 cgaaacgggt ccggaacttg tggttggccg ctcgcagcgc cgcctccgac tcgatgccga
    10201 gcttgcgccc gacgttcacc acgacgagga gcaggtcgcc gaactcctcc tgctgctcgg
    10261 ggctcgtcgc ggcgctcgcc agctccccga gctcctcggc caccttgtcg agcacgccgt
    10321 cgatcgatgg ccagtcgtag ccgagggccg acgcacgctc ctgcatctcc tggctcgcgg
    10381 cgagggccgg catcgacggg ctgatgccgt cgagcgcgct cttcgggacg ggcgccgagc
    10441 cgtcgccccc gctcccggcg gcctcggcgg cttccgcggc cgcctcgcgc tcgccggcct
    10501 tgattcgctc ccattgccgg ttcacgtcgg ccgacgttcg agcgaccgcg tcgccgaaga
    10561 cgtgcgggtg gcggcgcacg atcttgcgcg cgatcgaggc ctgcacgtcg gcgaggtcga
    10621 acacgcccgc ttcggccgcg agctgcgcat gcaggatcac ctggagcagc aggtcgccga
    10681 gctcctcggc gagggcgggc gtcgccccgt gctcgagcgc gtcgtacacc tcgtacgtct
    10741 cctcgagcag gtgcttgcgc agcgaggcgt gggtctgctc gcgatcccag gggcagccgt
    10801 cgggctggcg cagccggtcg ctgatgtacg gcatcgccca cggcccggcc gacgcgagct
    10861 ccggcgcgat cgggcgaacg tagagcgggt gttcaaggtc gcgcgcggtg agtgcgccga
    10921 tcgtggtcgc tgcgccgatc gtggtcgctc cgccactcgg agcgcccgag gtgtcgccat
    10981 cgaccggtgc gccgagcctc gccacggtgg catcggcggg atacagccgg gcgaggacgg
    11041 cgagcggatc gcgaccggcc gggccatggc gcccgggcag gggacgggca gcaccgtcgc
    11101 cggccgcccc gtcgctggcc gtgccgtcgg tggcgccgcc cagcaccgcg agcggcacga
    11161 tgagcagcgg tcgagatgcc tcgatcggcg ccgcggtcag gccgtcggcc gggacgatct
    11221 ggattcccgc cgagacgtcg aggccccagc gagcgcgcgc ctcggcgacg aggtcgtcga
    11281 gcatctgccg gatcaggcgg tgcccgagga cgacccgagg ttgtagacga tgtcggccgc
    11341 ttccttctgg cgcgtgtacc agaaggagaa gccggagtcc ttgaagatct tcagctgctc
    11401 ggccgtcgcc tccctggtct cctcgccgat gacgcgcagg aggtagtcgc cgtcgctggg
    11461 gatggtgacc acgctcgaca gcgagccgac gttcgtcgag aacacggcct tgtcgagctc
    11521 gtcggccagc tggccggtgg cgatccagcc gagatcgccg ccgtccctgg cctcggatcc
    11581 ctggctcgtg ttcttcgcca cacgcatgaa cgacgcgtcg tcgccgacat cggccttcag
    11641 ctggttgagg tacgccgtct cgccgtcgcc ggatgggcgg aggtactgga tgacgtacca
    11701 gccgagctcg cccttgaccg gcgcgatgag ctcgtcgggc ttgcgggtgg gctccaggac
    11761 gatgctgcgg atcacctcgt cgatcgctga ggtcgggtag atccagggct gcttgccgcc
    11821 ggtcccgacg gcggatccct cgtcggaatc ggtgcgggcc atcgcgtcga atcggctgat
    11881 gtccgcccgc agcgcgctgt acgccgcatc ggcctcggcc ttcgcctcga cccatgctgg
    11941 atcggtcagg ggcagctcgt ccgcgttggc gatgtcgtcc ttgggcgaga agacgatcca
    12001 gcggaccttc gcgccgacgt cgcccggggt gctcgaggtg ttgggctcgg gcaggtagat
    12061 ctcgaggacg tggcgctgct tgctcggctt cgagaggtcc gccacgatct tgtcggacag
    12121 cttcttgcgg atcacgtcgt tgcgcgccgc cgtgcggtag tcggcgagga ggatgccgtc
    12181 cttctcgatc cgggactgga aggtcgggtc cagctcctcg ggtgtcgatt cggtgtagcg
    12241 cccgatgcgg tacgcgccgt cctcgccctc gacgatctcc gttggggtgt tgaccggcgc
    12301 cgcgaacacg gccgccatga aggcctcgtc gtagccgctg tccttggaca gccagccgag
    12361 gttgccggcc tgcggcgcga ggcccgaatc cgatgccgtc ctggccacgt cctcccacga
    12421 ttcgccggcc ttgagccggg cgatcgcgcg ctgtgcgcgg tccagcgctg cccgcttctg
    12481 cgcctcgtcc acctcgccgg tgatggggtc ggcgctgggc tcgatctcga tcatgtagac
    12541 gtgccgctgc tcggaggtgg tcgcctcctg gtggagctgg tcatcgatgt ccgcatcgga
    12601 cacggtgatg ccgttctggt cggccagcgt gcccatgagc gcgttgtcga cgagccgctc
    12661 gagggtgaga ttcgcgagct gctggcggcg ctgggccagg aactggatct gctgctggcc
    12721 gtcggacggc gagatctggc ccttggccat cagcgtctgg atgcggctct cgatgtagtc
    12781 gaggcggaag ttctcgatca cgagccggcc gcgcaggtcg tccttcgtga tgaccttgcc
    12841 gttgaccgtc gccgcggcgc cgaagtggtc gtcgtaccag ctccaggccg tgtagccgac
    12901 gaggatgagc acggcgaggc cgatggcgat gaagaagccg aggttgatca ggctgttccg
    12961 gcggtcaccg gaatcccacc cggcgcggcc gcggcgtcgc gcgaccggtc tggctcggag
    13021 cgtcatgcga ggggagtgtc ctgttcggtg tggggcgcgc cgatcatgcc aggcgcgatg
    13081 gcggggccga atggtaccgc acgatcaggc gagggccgga acgacgatcc ggtcgacgaa
    13141 gcgctcggtc gcatggccgt cggcgacctc gaaggattcg tcgcggaagc gggcgatccg
    13201 atcgagatcg aacgcccctg cgcgcagcca ggccgcgagg tccgcggtcg tctcgaacac
    13261 gggccccggc acgcccgtgc ggtagtcgaa gtagaacccc cgctcgtcca tgtagtcgtc
    13321 gtggtccggc gcgaagaagg ccatcggccg ggtcaggagc gagaactcga agatcgcgct
    13381 cgagtagtcc gtgaccagga cgtcgctgac gagcatcagc tcgtggatgt cgggatggtc
    13441 ggagacgtcg atcgcgaacg acgccagctc cgctgggatc gggtgcgcct ggcgcacgaa
    13501 cgggtgcagg cgcagcagca cgatgtggtc ctcgccgagc acccgatgca gctccgcgaa
    13561 gtcgaggtcg gtgggtgacg ttgccttccg ggtggtcgcg ccccgaaagg tcggcgcgta
    13621 caggatcacg cgcttgccct cgggcaggtg gtagtgctca cggatcgacg ccttgatgcc
    13681 gggcacggcg tccccgaaca ggacgtcggt gcggggcatc ccgacatcgg tctcgaaccg
    13741 ctccagcggc gtgcggaatg cctccgcgta gtggaccgcg gcggtcttcg aggccatgag
    13801 gcagatgtcg tagttggagt ggatgtcgac cttggcgatg aggccctcgt ccgcgccgaa
    13861 cgtcttgtcg agcacgctgt agccgatctt cttgaacgcg cccgaggcgt gccatgtctg
    13921 gacgatcgtc gtgcccttgc gcggctcgat cacgtagacc gggaagtagt agtcgtcgac
    13981 gatgaacagc cgggccgtgg ccaggtgaaa cgccgcgcga atgttcgcga ggaacgaccc
    14041 gaaccaggtc ccccaggtga tcgccggcag gcgggcgacg acgctgtacg ggatcccgcg
    14101 ccggtcgagc tcggcgcgga tgaacgcgag gttgttggtc agatcggccg cagcggacgt
    14161 gccgaagacg actcggcgtc gcaggggcat gacgcgcgcc attgcgaacg tcgtgcgcac
    14221 cagcgcgatc ctgaaccgga ggattcgccg ccgtcggggt cgccgaggct cgtcggggga
    14281 tcgaggcgcg cgtcggcggc gccatggcat cctgggaatc ctggacctcc gggggacgcg
    14341 gggtgaacgg cgggtgactc gctggcgatg ataccgggcc agcccgggcc agcccggccc
    14401 agcccggccc agcccggccc agcgcggccc agcccgctac gcttacaccc aactgctgct
    14461 ggaggcgcgc atgacgttcg gcatggtggt gctcgcgggc ggcatcggcc ggcgaatcgg
    14521 ccgacccttc ccgaagcagt tcctgctcct cggcggcaag ccgctgatca tccacgtgct
    14581 cgagaaggcg cgggcgatcc cggagctcga gcggatcgtg atcacctgcc cggaggccca
    14641 tctcgacgag acggtccagc tcctgaagaa ccaccggatg gacaggcggt tcagctgcat
    14701 cgtcggcggc gcgacccgac aggagtccgt ctacaagggt ctccaggcgc tcgacggcgt
    14761 cgattccgtg gtgatccacg aggccgtccg gccgctggtg acgaccgagg agttccgcgc
    14821 cctgatcgcc agcccggacg aaaacgtgat gttcgggatc ccgatctcgt tcaccgtcct
    14881 gcgcggccac gaatacgtcg agggcctgct cgaccgcgag gagctggtca acgtccagct
    14941 gccccagaag ttcgaccggg ccaagctgct ggcggcgcac gaggcgtctc gcgccgcggg
    15001 cacgtcgttc accgaggacg cgagcctgtt cgtcgagcac agcggtgaga aggtccggat
    15061 cctcggcggc agcgagcgca acgtgaagat cacggtcccg gccgacatcg tcatggccga
    15121 ggccctgtat gccgattcca tcgggcgcgg tagctgacgg gtgggcagga cggcgctcgt
    15181 cacgggcgcg agccagggca tcgggcgagc gacggcggtt cggctcagcc gcgacccgtc
    15241 gatcacgaag ctggtcctcg tcgcccggag cgccgacggc ctcgcggcca cggccgaggc
    15301 ctgcgcggcg gatgcggaca agctcgacct ggtcgcgtac gacctcgccg acctcgacgg
    15361 catccccgac ctcgtggccg agatccacga gcgccacggc gggatcgacg tgctgttgaa
    15421 catcgccggc tacgccgagc ccaagtcgct cctcgacacg accgccaaga acctcgtcac
    15481 gaccttcacg gtcaacgtct tcgcgatgct gatcctgacc cgcgaggtcg tgcggaacat
    15541 gcagggccgg ccgaccaaga tcgtgaacat cgcctcgacc gcgggcatca ccgcccgacc
    15601 cggctgggtt gcctacgcct cgtcgaaggc cgctgtcgtg tcgctgtcgc agaccctgtc
    15661 cgaggagctt gccggcacgg gcgtgaagat ctacacgatc tcgccgggcc ggaccgcgac
    15721 cgagctgcga cggaagctcg cgcccgagga ggatccgagc acgatcatgc agcccgatgc
    15781 cgtcgcggac gtcatcgcca tgctcgtggg cgagggcgag cgcacgctgg acggccagaa
    15841 catcatcgtc cgccagcaga tctgacccgg ccgatggggc gccggcaccg ccgatgaacc
    15901 ggctcgagca taccctcgcc tccgccgttc tccgcgtcct cggcttcttc ttcgaccggc
    15961 tgccgattcg gcaccgcgtc gttctcacga ccgcccgcgt ggcccatctc gagggcaacc
    16021 tcgcctacct gcacgcggcc atccggcggg cccgcccgga cgtgccgctg gtcacgctgc
    16081 tggagccgta cagctacggc ctggccggca agctcgccta cctcctgcgc ctcgtgcgcg
    16141 ggacatacct cctgcgcacg tcgtccctct tcgtcgtcga caacgcatac ctgccggtcc
    16201 atgtcgcgcc gcaccggcgc gggacgacgg tggtccaggt gtggcacggc gtcggggcgc
    16261 tgaagcgctt cggggcggac acggcgaagg ggctcgacga gccggagcgc tcgttcctgc
    16321 atcaccacta cgacttcgtg gtgtgcgccg gcgaggcgtc ccgcgcgccg tacgccgcgg
    16381 cgctgcggac gccgatcgag cgggtccagc cgctcggtgc agcccgaact gacttcttct
    16441 tcgaccccgc ggccatcgtg gcggcgcgcg agacgacact ggccacctac ccggcactgc
    16501 gcgcgggccg cgtcgtgctc tacgccccca cgttccgcgg ccgcggccgg ggcaagcgcg
    16561 ccgctcccgg attcgacgct gcggccgtcc gcggcgcgct cccgccgggc cacgtcctcg
    16621 ccctcaagtc gcatcccaac ctcgatccgg cgctggtggc tgcggccggc ttcgacgtgg
    16681 tcatcgagcc gtccgtcgag ctcaaccagg tgctcgccgc gaccgatgtc ctcgtcacgg
    16741 actactcctc ggcgatctat gagtgggcgc tgctccggcg accgttggtg ctgctcacgg
    16801 aggacctcga ggcgtacgag cgcgacccgg gcatgtacct cgacgcccgc accgagctga
    16861 tcggcgagcg catcgacgat ccggccgacc tggccccggc gatcctccgc gcgaccgtcg
    16921 atgacgcggc gtggacggcg ttcatcgccc gccgcatcga ggcctgcgac ggcggggcca
    16981 gcgagcgctt cgtcgaacgg ttcctgtgac cgggccgggg aagcgaccgc gcttctcgat
    17041 cgtcaccgcc gtctacgacg tcgagccgta cctgcccgac ttcatcgcct cgatcgaggc
    17101 gcagcgggtg ccggcggcgg atctcgaggt cgtcgccgtc gacgacggtt cgaccgatgg
    17161 ctccctcgac ctgctcctcg actgggcgcg ccggagccgc ctgcgggtga aggtgttcac
    17221 gaagccgaac ggcggccagg gctcggcgcg caacctcggg ctgacccacg ccaccggcga
    17281 gtgggtgaac ttcgccgatc ccgacgacat gctcgacccg gacttcctgc gcgtcgcggc
    17341 gcgcttcgcg gcagccaacc ccgaggtcga gatcatggcc ggcaagccga tttccttgat
    17401 cgaaagcgtc gggcgaatcg ccgacaacca tccgcgccgg caccagtacc ggcgcgggaa
    17461 tcgcgtcgcg aacctgctcg acgagccgaa tgtgttcagc ggcagcgcga cggtgagcct
    17521 ttttcggctg gaccgggtcc gagggatgga cctgcgcttc gacccccgga tccggcccaa
    17581 cttcgaggac ggtcacttcg ccatccacta cctgctcgcc ctcgacgtcc cgcgggtcgg
    17641 cctcctgcgt gacgcgcgct acctgtatcg ccgccgggcg gcgggcacct cgacgctgca
    17701 gggcagcctc gcccaccccg gccggttcac cgacgtcctg gagcatggct acaacgaggt
    17761 gctcgacgcc gcgacggcac tgcacggcag catcccggcg tggctccagc aggtgatcgt
    17821 ctatgagctg tcgtggtacc tctcggccga cgagaagatc acctcggaca tcgtcctcgc
    17881 cgacgagctg gtgccccggt tccacgagct gatggcccgg atcgtgtccc gcctcgagcc
    17941 cgacgtcgtg gccgctcacc gggtccgcaa gctgcgcgtc gtctgggccg acatcctcgc
    18001 ccacggctac cgcgaggaga cctggcacat gccgttcgtc gtgcgcggca agctcgatcg
    18061 cgagatgggc ctccggcggc tcggctaccg gttcgtcggg gcgcagcccg acgagcgctt
    18121 cgtagtggac ggcgtcgagg tcgcgcccgc gttcgccaag acgcgcgcac accgctacta
    18181 cggccgcgtg ctgatcgacg agcgaatcat ctggattccg tgggggacga cgatccaggt
    18241 gttcctcgac ggcgtcgaga cgatgacccg acacagatgg gcgccgcgct tcgggcggcg
    18301 cggccggcgc gcccttgccg agcgcctccc gatctatcgc cggctgcccc tgcccttctt
    18361 cgtcgc
//
LOCUS       DATBSW010000162         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_825834, whole genome shotgun sequence.
ACCESSION   DATBSW010000162 DATBSW010000000
VERSION     DATBSW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825834"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VIF63_04540"
     CDS             complement(<1..715)
                     /locus_tag="VIF63_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238683.1"
                     /translation="MHAPEPSRRRTVELDPLIGRDADIERVEYALARTRLVTITGPGG
                     TGKTRLASVIVERFRQAGEEAWFIDLTTVPGPELVAAAIVSALGLRGASLEPEDIVTG
                     AFRDRAVLLALDNVEQIPVVGPLVDGWLRELPGLRLLLTSRIPVRVGGEIEVHLETLG
                     VPEADDQGSVEAAPASALFLRRARSIGRLDALDPEAARAVAALVRRLDGLPLALELAA
                     ARTRIMSPAEILARFEREGP"
     gene            878..1324
                     /locus_tag="VIF63_04545"
     CDS             878..1324
                     /locus_tag="VIF63_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8238684.1"
                     /translation="MNKVLLAGRLTRDPEMRALASGKHVTQFSVATNEYAGNGKERPE
                     YHNIVTWDRLAEVCGRYLGKGQQVAIEGRIQTRTWDDEKGLRHWKTEIVATTVEMLSG
                     RKKKDYAAETSADAIEAAAGGEEATESESEAPSVPADEPIAEELAA"
     gene            complement(1392..2285)
                     /gene="rsmI"
                     /locus_tag="VIF63_04550"
     CDS             complement(1392..2285)
                     /gene="rsmI"
                     /locus_tag="VIF63_04550"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY8238685.1"
                     /translation="MRGGADAAPAELPSTSGGTLFIVSTPIGNLGDVTLRALEVLRTV
                     PLIAAEDTRMASRLLGRYEIGTRTTSFHARSGPGRLAALLTHLRGGWDLAFVTDAGTP
                     VVSDPGGELVAAWAEEGGRVVPIPGASAVLAAVAASGIAGPRWGFEGFLPRSGRERKE
                     RLARIAADDRSTVLFEAPTRLAATLRDLAATCGADRTAAVCRELTKLHEQIARGTLSE
                     LVASVEAGTIPARGEIVIVVGSMAGVERPEAVADHAVAIEAARAEVVKRVAAGESPAS
                     AAKAVATATGLPRRALYPAGH"
     gene            complement(2289..3209)
                     /locus_tag="VIF63_04555"
     CDS             complement(2289..3209)
                     /locus_tag="VIF63_04555"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY8238686.1"
                     /translation="MSTVGALPTDDAGKPLFELPDELAIDTDVARRVIAGFIRGQLEQ
                     AGFERCVLGLSGGIDSALVAYLVAEAIGADKLLCVLMPYRTSSPASRGDAETIVADLG
                     CASELVEITAMVDGYFGSGAGAGAGGEGARDASALRRGNLMARMRMSVLYDRSVAFGG
                     LVVGTGNKTESLIGYTTIFGDNACAFNPIGDLYKSQVRQLAAAVGVPHQVITKAPSAD
                     LWPDQTDEGEGSFSYPVLDRVLYWRIDRRRSAEELEAMGFDRALVERVERLVATSEFK
                     RQVPPIAKLGPRTAGVDYLYPRRRPGSART"
     gene            complement(3206..>3419)
                     /locus_tag="VIF63_04560"
     CDS             complement(3206..>3419)
                     /locus_tag="VIF63_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238687.1"
                     /translation="LELQARELGRIIAERAGLAADTTAESGAGPGLDVAPERKRARAV
                     ARGAESDARAPEAGPPEPIRLRSRRR"
BASE COUNT          488 a         1252 c         1226 g          453 t
ORIGIN      
        1 ccggtccctc gcgctcgaac cgggcgagga tctcggcggg gctcatgatc cgtgtccggg
       61 cggcggccag ctccagggcg aggggcagcc catcgaggcg gcggaccagg gcggcgacgg
      121 cccgcgcggc ctcgggatcg agcgcgtcga ggcggccgat cgagcgggcc cggcgcagga
      181 agagcgcgct tgccggagcg gcttccaccg atccttgatc gtcggcctcg ggcacgccca
      241 gggtctcgag atggacctcg atctcgccgc cgacccggac cgggatccga gaggtcagca
      301 gcaagcgcag ccctgggagc tctcgcagcc aaccgtcgac cagcggcccg acgacaggga
      361 tctgctcgac gttgtcgagg gcgaggagga cggcacgatc gcggaatgcg ccggtgacga
      421 tgtcctccgg ttcgagactg gcgccccgga ggcccagagc ggacacgatc gcagccgcca
      481 cgagctcggg tcccgggacc gtcgtgaggt cgatgaacca ggcttcctcg ccggcctggc
      541 ggaaccgctc aacgatcacg ctcgcgagcc gggtcttccc ggttccgccc ggtccggtga
      601 tcgtgacgag gcgcgtccgg gcgagtgcgt actcgacccg ctcgatgtcg gcatcacggc
      661 cgatgagcgg gtcgagctcg acggttcgac ggcgggaggg ttcgggcgcg tgcatcgctg
      721 ccgcgcagcg tagagccctc ggacaagggc ttggccttca agtgtcgcga taagccgcgg
      781 tcgaccgccc gataagcgtc gctcgctagg gtcgcggcac cgccgcacgc agggtcggcc
      841 cgtgtcccgt cggtcgaccg ataaggagtc atggaccgtg aacaaggtcc tcctggccgg
      901 ccgcctcacg cgcgatcccg agatgcgcgc gctggcgtcg ggcaagcacg tcacgcagtt
      961 cagcgtggcc accaacgagt acgcgggcaa cggcaaggaa cgccccgagt accacaacat
     1021 cgtgacctgg gaccgcctcg ccgaggtctg tgggcgttac ctcggcaagg gccagcaggt
     1081 cgccatcgag gggcgcatcc agacccggac ctgggacgac gagaagggcc tccgccactg
     1141 gaagaccgag atcgtcgcga ccacggtcga gatgctgtcg ggtcgcaaga agaaggacta
     1201 cgccgccgag acgtcagccg atgccatcga ggcggccgcg ggtggcgaag aggcgaccga
     1261 gagcgagtcg gaggcgccct ccgtgcccgc cgacgagccg atcgccgagg agctcgcggc
     1321 ctgatcccga ctcccccact ccgcccgggc ggcgccggca cctccctcgg tgccgtccgg
     1381 gcggcgccct ctcaatgccc ggctgggtac agtgcccggc gtggcaggcc cgtcgcggtg
     1441 gcgacggcct tcgcggccga cgccgggctc tcgcctgcgg cgacgcgctt gaccacttcg
     1501 gcacgggcag cttcgatcgc gaccgcgtgg tccgccaccg cctcgggtcg ttcgacgccg
     1561 gccatcgatc cgacgacgat cacgatctcg cctcgggctg ggatcgtgcc agcctcgacg
     1621 ctggcgacca gctcggacaa cgtgccgcgc gcaatctgtt catggagctt ggtgagctcg
     1681 cggcagaccg cggctgtccg gtccgcgccg cacgtcgcgg cgaggtcgcg cagcgtggcc
     1741 gcgagtcgcg tcggcgcttc gaacagcacc gttgagcggt cgtcggccgc gatgcgcgcc
     1801 agccgctcct tccgctcgcg ccctgatctc ggcaggaagc cctcgaagcc ccagcgtggt
     1861 ccggcgatcc cgctcgcagc gaccgcagcg agcaccgccg atgcgcctgg gatcggaacg
     1921 acgcgcccgc cctcctcggc ccaggccgcg acgagctctc cgcccgggtc gctcacgacg
     1981 ggcgttcccg catcggtcac gaacgcgagg tcccacccgc cgcgaaggtg cgtgaggagc
     2041 gcggcgagcc gcccgggccc gctgcgtgcg tggaagctcg tcgttcgggt tccgatctcg
     2101 taccgcccga gcagtcgcga agccatccgc gtgtcctcgg cggcgatcag cggaacggtc
     2161 cgcaggacct cgagggcgcg gagcgtgacg tcgcccaggt tgccgatcgg ggtggagacg
     2221 atgaagagcg tgccgccaga cgtcgaagga agctccgccg gcgcagcgtc ggccccgccc
     2281 ctcacggact aggtgcgcgc cgagccgggg cggcgccgcg ggtacaggta gtcgacgccg
     2341 gccgttcgcg gcccgagctt ggcgatcggc ggcacctggc gcttgaactc cgaggtggcc
     2401 acgagccgct cgacgcgctc gacgagcgcc cggtcgaagc ccatcgcctc gagctcctcg
     2461 gcgctccgcc gccggtcgat gcgccagtac aggacgcggt cgagcaccgg atagctgaac
     2521 gagccctcgc cctcgtcggt ctggtcgggc cagaggtcgg cgctgggcgc cttcgtgatc
     2581 acctggtggg gcacgccgac cgcggcggcc agctggcgga cctggctctt gtacaggtcg
     2641 ccgatcgggt tgaaggcgca ggcgttgtcg ccgaagatcg tcgtatagcc gatcagcgac
     2701 tcggtcttgt ttccggtccc gaccaccagg ccgccgaacg ccaccgaccg gtcgtacagc
     2761 acggacatcc gcatccgggc catgagattg ccccgtcgca gcgccgaggc gtcccgcgcg
     2821 ccctcgcctc cggcgcctgc acccgcgcca ctcccgaagt agccgtcgac cattgccgtg
     2881 atctcgacga gctcgctcgc gcagccgagg tcggccacga tcgtctccgc gtcacctcgc
     2941 gacgcgggcg acgacgtccg atagggcatg agcacgcaca gcagcttgtc cgcgccgatc
     3001 gcctcggcga cgaggtacgc cacgagcgcc gagtcgatcc cgccggacag ccccagcacg
     3061 cagcgctcga agccggcctg ctcgagctgg ccgcggatga acccggcgat cacgcggcgc
     3121 gccacgtcgg tgtcgatcgc cagctcgtcg ggcagctcga acagcggctt gccggcgtcg
     3181 tcggtgggca gcgcgccgac ggtgctcatc ggcgccggga cctgaggcgg atcggctcgg
     3241 gcggacctgc ctcgggcgcc cttgcatccg attcggcccc ccgcgccacc gctcgcgctc
     3301 gcttccgttc cggcgcgacg tcgaggcccg gcccggcacc cgactcggcc gtggtgtcgg
     3361 cggcaaggcc ggcccgctcg gcgatgatcc ggccgagctc gcgcgcctgg agctcgagc
//
LOCUS       DATBSW010000163         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_649430, whole genome shotgun sequence.
ACCESSION   DATBSW010000163 DATBSW010000000
VERSION     DATBSW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649430"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VIF63_04565"
     CDS             complement(<1..279)
                     /locus_tag="VIF63_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8238688.1"
                     /translation="MELRPATPDDEWLTVALETDPRVMAELGGPWTVDEAKAAHLRRT
                     GPGAKDGSWWLAILPGPGQPPIGTIGVWASDWAGEPISETGWMILPEHQ"
     gene            complement(282..2360)
                     /locus_tag="VIF63_04570"
     CDS             complement(282..2360)
                     /locus_tag="VIF63_04570"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY8238689.1"
                     /translation="MSASMLSRLTGPADLKGLTEAQLAELAAEIRETIIGTVAKTGGH
                     LGSSLGVVEIAIALHRFLDSPRDKIVWDTGHQAYAHKLLTGRLERFGTLRQIDGVGGF
                     PRRSESEHDVFDGGHAGTGLSIAEGLAAARDIRHGSERIAVVVGDAALMTGLSLEALN
                     DIGHRQTPMLIILNDNEMSISATVGAFSTYLSRIKVSRPWLSSKSAYERLVGGIPVIG
                     APMVMWSQRLRRSVVNLASPGQLFEDLGITYIGVVPGHDIHALLETFRRVSRLKGPVL
                     VHVRTQKGRGYLPAETDQVSFHGAALPPMELAPTVDAFDGTRARVPTASGAREIRPGT
                     PHGAGSNGDALSPPTPPSAGKKPPSYTSIFVSELIELANDDDRIVAITAGMPTGTGLS
                     KFQAAFPDRFQDVGIAEQHAVTMAAGLAIAGMRPIVALYSTFLQRAFDQTVHDVCQND
                     LPVLLAVDRAGLVGEDGTSHQGMFTIPAQRQLPYLVVASPKDEQELRSLVHTAFEQEH
                     PFALHYPRDPGFGLPDVTPPRAIPVGQAEVLREGEDILFVGFGPITMRAVEVADALAE
                     DGWSIGVVNARFVKPLDRQLILDQARGKRLVVTFEESVVTGGFGSAVLEAIEEARLTD
                     ATLRETSVRILGIPADRFVDHGSVVDLRRRIRLDVPGLTEQVREAVATLALVPSHPEA
                     AHAPEAARAV"
     gene            2486..3358
                     /locus_tag="VIF63_04575"
     CDS             2486..3358
                     /locus_tag="VIF63_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238690.1"
                     /translation="MFIRIIAGVAIAAVIVGCSVTLPSEPSSAASGVPSTTASPARPT
                     PTPVGSFVVVGALDDDGVPTSVEGVAVLRGDALRAAISGSIDSSAILGGGWFHGPLST
                     AYCTLQLFEEDRAVSICNSIGLFEHRVGGSPVRMAAGDVRDAANVSTAVDRPVVLRLH
                     THDARCVPQDKGCDLRPVALDIAWLGDAPLEPPPPRVAGTPPPDGITRAKAIQRARAE
                     SLAHRSPLVLRSAVASPIWAVLPGLEASEGDIWVWTVVFEADFTSPCGDCQSSDTEEV
                     FLEYLTGAFIMARQ"
     gene            3369..>4126
                     /locus_tag="VIF63_04580"
     CDS             3369..>4126
                     /locus_tag="VIF63_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8238691.1"
                     /translation="MRTEWVDAAGARVHVRRWGPADGRPLLFLHALGPAASGALIDVG
                     VGPLADAGWSIAAPDMPGFGRSPVIAPDGYATARLADLAWAVADPLGWQEIVLGGHSW
                     GGANAIHAAAARPERVRALVLVDSGHVDYANAPGVDLTASMDQMIEQAEQGRLRARDR
                     AGVASDLELPIDDPVVDSFLEGVMDDGAGGLITRTRGVARGPAMYHLARARSTEQWPA
                     IAAAGIPTLLLLATEPPDLRERNEAAVARFRAAIP"
BASE COUNT          575 a         1418 c         1488 g          645 t
ORIGIN      
        1 ctggtgctcg ggcaggatca tccagcccgt ctcgctgatc ggctcgccgg cccaatccga
       61 cgcccacacc ccgatcgtgc cgatcggcgg ctgtcccggg ccgggcagga tggccaacca
      121 ccagctgccg tccttcgcgc ccgggcccgt gcgccgcagg tgcgcggcct tcgcctcgtc
      181 gaccgtccac gggccgccga gctcggccat gacccgagga tcggtctcga gcgcgaccgt
      241 cagccactcg tcgtcgggcg tggccggccg cagctccacg ctcagacggc ccgtgcggcc
      301 tcgggcgcgt gcgctgcctc ggggtgcgac ggcacgaggg cgagggttgc gacggcctct
      361 cgaacctgct cggtgagccc cggaacgtcc agtcggatgc gccgtcgaag gtcgacgacc
      421 gagccgtgat cgacgaaccg gtccgccggg atgccgagga tgcggacgct cgtctcgcgc
      481 agtgtcgcgt cggtgagacg ggcctcttcg atcgcctcga ggaccgcgct gccgaagcca
      541 ccggtcacga cgctctcctc gaaggtcacg acgaggcgct tgccgcgcgc ctggtcgagg
      601 atcagctggc ggtcgagcgg cttcacgaag cgggcgttga ccacgccgat cgaccagcca
      661 tcctcggcga gggcatcggc gacctcgacg gcgcgcatgg tgatcggccc gaagccgacg
      721 aacaggatgt cctcgccctc acgcagcacc tcggcctggc caaccgggat ggcgcgaggc
      781 ggcgtgacgt cgggcaggcc gaagcccggg tcgcgcgggt aatggagcgc gaacgggtgc
      841 tcctgctcga acgcggtgtg gaccagcgag cgaagctcct gctcgtcctt cgggctcgca
      901 acaacgaggt acggcagctg gcgctgggcc gggatcgtga acatgccctg gtgcgacgtg
      961 ccgtcttcgc cgacgagccc agcgcggtcg accgcgagca ggacgggcag gtcgttctgg
     1021 cacacgtcgt ggacggtctg gtcgaaggcc cgctggagga acgtggagta cagcgccacg
     1081 atcggccgca tgcccgcgat cgccaggcct gcggccatcg tcaccgcatg ctgctcagcg
     1141 atcccgacgt cctggaagcg gtccgggaat gccgcctgga acttcgagag gccggtgccg
     1201 gtgggcatgc ccgcggtgat cgcaacaatg cggtcgtcgt cgttcgcgag ctcgatgagc
     1261 tcggacacga agatcgacgt gtagctcggc ggcttcttgc cggcgctcgg cggcgtcggc
     1321 ggggacaggg cgtcgccgtt cgagcccgcg ccgtgggggg tccccggccg gatctccctc
     1381 gcgcccgagg cagtggggac acgcgctcgc gtgccgtcga atgcgtcgac ggtcggcgcg
     1441 agctccatcg gtggcagggc cgcgccgtgg aacgacacct ggtccgtctc ggcgggtagg
     1501 tagccgcggc ccttctgggt gcgcacgtgg acgaggacgg gccccttcag ccgggagacg
     1561 cggcggaatg tctcgagcag ggcgtggatg tcgtggccgg gcacgacccc gatgtaggtg
     1621 atcccgaggt cctcgaacag ctggcccggg ctcgcgaggt tgacgaccga tcgtcggagc
     1681 cgctggctcc acatcaccat cggcgcgccg atcaccggaa tccccccgac gaggcgttcg
     1741 tacgcggact tgctcgacag ccagggccgg ctcaccttga tccgcgacag gtaggtcgag
     1801 aaggcgccga cggtcgcgct gatcgacatc tcgttgtcat tgaggatgat cagcattggc
     1861 gtctggcggt ggccgatgtc gttgagcgcc tcgagcgaca ggccggtcat gagcgccgcg
     1921 tcgccgacga cgaccgcgat ccgttcgctg ccgtggcgga tgtcgcgggc cgccgcgagg
     1981 ccttcggcga ttgacaggcc ggtccctgca tggccgccgt cgaacacgtc gtgctcgctc
     2041 tcgctgcggc gtgggaagcc accgacgccg tcgatctggc gcaaggtccc gaagcgctcg
     2101 aggcgcccgg tcagcagctt gtgggcgtag gcctggtggc cggtgtccca gacgatcttg
     2161 tcacgcggcg agtcgaggaa gcggtgcagc gcgatcgcga tctcgaccac gccgaggctc
     2221 gagccgaggt ggccgccggt cttggcgacg gtgccgatga tcgtctcgcg gatctccgcg
     2281 gcaagctccg cgagctgcgc ctcggtgagt cccttgaggt cggcgggccc ggtcagccgg
     2341 gacagcatgg atgcggacac gttgctttgg cgggctccaa gtgcgagtct gcgagacgcg
     2401 aaccgtatgt gtcgatgtga cgcgccggtc cggcaaagtg taggacgagg ccctcagcga
     2461 aggatacttg gtaccgccag tgggcatgtt tatcagaatc atcgcgggtg tcgcaattgc
     2521 cgcggtgatc gtcggctgct cggtgacgct tccctcggag ccgtcgtccg cagcttcggg
     2581 cgtgccatcc acgactgctt cgccggcgcg gccgacaccg accccggtcg gctcgttcgt
     2641 cgtcgtcggg gcgctcgatg acgacggcgt cccgacatcc gtggagggcg ttgcagtcct
     2701 gcgcggcgac gcgcttcgcg ccgcgatcag cggaagcatc gattcgagtg ccatcctcgg
     2761 cggtggctgg tttcacgggc cgctgagcac tgcctactgc acgctgcagc tgttcgagga
     2821 ggatcgagcc gtatcgatct gcaactccat cgggctcttc gagcaccgtg tcggcggcag
     2881 cccggtccgg atggccgccg gcgatgtccg cgatgccgca aacgtgtcga ctgctgtgga
     2941 ccggccggtc gtcctgcgac tgcacacgca cgacgcccga tgcgtgcccc aggacaaggg
     3001 ctgcgacctg cgcccggttg ccctcgacat cgcctggctc ggcgatgctc cgctggagcc
     3061 gccgccgccg cgcgtggccg gcactccgcc gccagacggg atcaccaggg cgaaggccat
     3121 ccagcgcgca cgcgccgagt cactggcgca tcgatcgccc ctcgtcctgc gctccgccgt
     3181 agcctcgccg atctgggccg tgctgcccgg cctggaagcg tcggagggcg acatctgggt
     3241 ctggaccgtc gtgttcgaag ccgatttcac ctcgccgtgc ggcgactgcc aatcgtccga
     3301 tacggaagag gtgttcctcg agtacctgac cggggcattc atcatggcgc ggcaataggc
     3361 tgaaagccat gcgaaccgaa tgggtcgatg cggcaggcgc gagggtccac gtccgccgct
     3421 gggggcctgc cgacggccgc ccgctccttt tcctgcacgc gctgggacct gcggcatccg
     3481 gggcgctgat cgatgtcggg gtggggccgc ttgcagatgc cggctggtcg atcgcggcgc
     3541 cggacatgcc cggattcggg cggtcgcccg tcatcgcgcc cgacggctac gccaccgcgc
     3601 gcctcgccga tctcgcatgg gccgtcgccg atccgctcgg ctggcaggag atcgtcctcg
     3661 gcgggcacag ctggggcggc gcgaatgcga tccatgccgc tgcggcgcga cccgaacggg
     3721 tgcgggcgct ggtcctcgtc gacagcggcc atgtcgacta cgccaatgcc ccgggtgtgg
     3781 acctcacggc gtcgatggac cagatgatcg agcaggccga acagggccgg ctgcgggctc
     3841 gcgatcgagc gggcgttgcc agcgacctcg agctgccaat cgacgatccg gtggtggatt
     3901 cgttcctgga gggcgtcatg gacgatggcg ctggcggcct gatcacccgc acccgaggtg
     3961 tcgcccgcgg cccggcgatg taccacctcg cccgcgcccg ctcgaccgag cagtggccgg
     4021 cgatcgccgc cgcggggatc ccgaccctgc tccttctcgc caccgagccg cccgatcttc
     4081 gcgagaggaa cgaagcggcg gtggctcgtt tccgtgccgc gatccc
//
LOCUS       DATBSW010000164         6555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_15191, whole genome shotgun sequence.
ACCESSION   DATBSW010000164 DATBSW010000000
VERSION     DATBSW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15191"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VIF63_04585"
     CDS             complement(<1..699)
                     /locus_tag="VIF63_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY8238692.1"
                     /translation="MTDRTFGGVELTRVAPAATPGPLDDTFYDLVEARFRRVVEEEPT
                     YATHLGIHAWDDRLPDPSRERILGDIEADKGHLAKVEALDPAGLSAEARFERDLELHH
                     VRLSLYRGDTIRTWERRSTGASGIGDAIFPLFTRDFAPLPERLAAITARLEAAPRYLR
                     DHRTRAVVPQVGPWLEVELTASASMPSFLDEVVAATDAAGPAVSTAERGRLVRAVERT
                     KVAIEDQAEWIHEIL"
     gene            complement(726..1871)
                     /locus_tag="VIF63_04590"
     CDS             complement(726..1871)
                     /locus_tag="VIF63_04590"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEY8238693.1"
                     /translation="MSLIDLRSDTVTKPTPEMRKAMADAEVGDDVFGDDPTVNALEER
                     AAELLGKEAALFVTSGAQGNLVAQMAHVGRGQEVIAGTHGHIMLHEAASYAVVVGASV
                     RPIDEQPDGTLDLQQVREAFRDPTDVHDPITGLVVFENASAHANNQPVGSAYVRSLAA
                     VAHEGGVPLHIDGARFWNSVVALDEDPRQLAGPADSVTFCLSKGLACPVGSLVVGSKD
                     FVWKARRARKLLGGGMRQVGVLAAAGLVALRDGDAGMIDRLADDHANARYLAEALADI
                     PGIRSPGETAQPDGDRLDPGRVRTNFVLFRVERDREAFLAELERCGVLMLSFMPHNGQ
                     IRAVAHYGIERSDIEATIRAVREALDELPPMPVGTSSSSRAYAGAVG"
     gene            1923..1999
                     /locus_tag="VIF63_04595"
     tRNA            1923..1999
                     /locus_tag="VIF63_04595"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1957..1959,aa:Asp,seq:gtc)
     gene            2153..2749
                     /locus_tag="VIF63_04600"
     CDS             2153..2749
                     /locus_tag="VIF63_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238694.1"
                     /translation="MIGSIGSRLRLVLRQPGLAMRSAGAVDGLAEFPEIDFVAYSTSE
                     RLSGRVRLDSARLTDMLNAHREFVLVDALAERLPDGGSMVVPEILVGRHELALVRVGG
                     PRGDRAKRVPTTTHRLVIRTGRYLVAGRLHSNAGEDPLAALRARDPMVPLTDAAIMFR
                     FGPDIVEEPGGTIVVNRDLVEWVRQGELDARSLDLPAN"
     gene            2838..3323
                     /locus_tag="VIF63_04605"
     CDS             2838..3323
                     /locus_tag="VIF63_04605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8238695.1"
                     /translation="MDEFRAEGRGGADDRSAIGRYLREHSLAWLSDAAFRDFTGAIRA
                     EQFEDTPRPAGFVPDTELWLVDGKAWLGRVAVRHRLTPNLLEMGGHIGYDVRPSARRR
                     GHATAMLREALVVARDLGIGPALVTCDVGNVGSRAVIERNGGVLEDERAGKLRFWVPT
                     D"
     gene            3337..4629
                     /locus_tag="VIF63_04610"
     CDS             3337..4629
                     /locus_tag="VIF63_04610"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8238696.1"
                     /translation="MAIEPNRGPLAGIRVIDCSTVLAGPYCTMLLGDLGAGVVKIEPP
                     EGDATRGWGPPWVGSESDGSRTAAYYLAVNRNKRSIRLDLSTEAGREVLRRLLRGGDA
                     VIENYRVGGFARLGFSDEVLEALNPHLVHLAISGFGTRGPDAAKPGYDFVIQAVGGLM
                     SITGEPDGRPMKVGVAISDVVSGLFGAVSVIAGLLARERDSTGDGSRDHGEGGHAGTI
                     NRVGSRQPVGQRIDLSLLQSTLAVLVNQAQNALVTGAAPKRRGNAHPSIVPYETFTTA
                     DGEIAIGVGSERQWARLGPAIGLPEIATEARFATNADRVEHRDELIPILAARFATASN
                     ESWLTRLDDAGIPAGPILDLPAALETPQAQALGSRVRMDHPVLGRVDQVGIPFELSAT
                     PASIRTAPPMLGEHTDEILREAGYRQPEIDALRAAGVV"
     gene            5033..5854
                     /locus_tag="VIF63_04615"
     CDS             5033..5854
                     /locus_tag="VIF63_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8238697.1"
                     /translation="MADSRERWALILGGSSGMGEATARALAADGYNICGIHLDFRASL
                     ERVERLKADLVALGRDVLYINMNAADDEKRAAALAELGKRFEAARAAGREPYVRVVMH
                     SLAFGSLVPFISEDPKGAVDRKKMEMTQDVMANSLVYWVQDLYRGGFLAEGSKIYAMT
                     SEGSTRVVPSYGVVSSAKAALESHIRQLAMELGRLKSGITANAIRAGVTMTPALMKIP
                     EHDMIIASATARNPTGRMTTVQDVANAIVALSGEGTGFISGEVIGVDGGEYVTGS"
     gene            5851..6423
                     /locus_tag="VIF63_04620"
     CDS             5851..6423
                     /locus_tag="VIF63_04620"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8238698.1"
                     /translation="MSEPGAGSIRPFYADWAGYNRRIIDAVARMTSDDLALQAPGTEH
                     WPIWAICGHMAGTRVFWLCDVFGEAGIEQTPWPNGTLGPGWEDDLSVPRSAEELVWAL
                     TSTWQVIEGLLGRWTPDSMDMTAQRNKAGRAPEIHTRQSILLRMITHEGYHVGEINIA
                     LGAAGREQIDPWPGREWQADAPRSLREGGS"
BASE COUNT          935 a         2308 c         2308 g         1004 t
ORIGIN      
        1 caggatctcg tggatccact cggcctggtc ctcgatcgcg accttcgttc gctcgaccgc
       61 cctgacgagc cggccgcgct cggccgtcga cacggccggc ccagccgcgt ccgtggcggc
      121 gacgacctcg tccaggaacg agggcatgct cgcgctcgcc gtgagctcga cctcgagcca
      181 cggcccgacc tggggcacga ccgcccgcgt ccggtggtcg cgcaggtagc gcggcgccgc
      241 ctcgagccgg gcggtgatgg cagcgaggcg ctcgggcagc ggcgcaaagt cacgggtgaa
      301 cagcgggaag atggcgtcgc cgatccccga tgcgccggtc gagcggcgct cccaggtgcg
      361 gatcgtgtcg ccccgataga gcgacaggcg gacgtggtgc agctcgaggt cccgctcgaa
      421 tctcgcctcc gcgctcagtc ccgccggatc gagcgcctcg accttcgcca ggtggccctt
      481 gtccgcctcg atgtcgccga ggatccgctc ccgggacgga tccggcaggc ggtcgtccca
      541 ggcgtggatg ccgaggtgcg tagcgtacgt cggctcctcc tcgaccacgc gcctgaagcg
      601 cgcttctacg aggtcataga acgtgtcgtc gagggggccg ggcgttgccg cgggcgcgac
      661 ccgcgtgagc tcgacgcccc cgaacgtgcg atcggtcatg tgggagctcg cgggggagcg
      721 gacggttacc cgaccgcgcc cgcgtaggcg cgcgacgagc tggacgtccc gaccggcatg
      781 ggcgggagct cgtcgagggc ctcgcgaacc gcgcggatcg tcgcctcgat atcggatcgc
      841 tcgattccgt agtgcgcgac cgcgcgaatc tggccgttgt ggggcatgaa cgacagcatc
      901 agcacgccgc accgctcgag ctcggcgagg aaggcctctc ggtcacgctc cacgcggaag
      961 agcacgaagt tggttcgaac acgccccgga tcgagtcgat caccgtcggg ctgcgcggtc
     1021 tcgcccggcg agcggatgcc gggaatatcc gcgagtgcct cggcgaggta tcgggcgttc
     1081 gcgtggtcgt ccgcgagtcg atcgatcatg cccgcgtcgc cgtcgcgcag cgccacgagc
     1141 cccgcggccg cgaggacccc gacctggcgc atgccgccgc cgagcagctt ccgcgcccgg
     1201 cgcgccttcc agacgaagtc cttcgagccg acgaccaggc tcccgaccgg gcaggcaagg
     1261 cccttcgaca ggcagaacgt gacgctgtct gcgggtccgg ccagctggcg ggggtcttcg
     1321 tcgagcgcca cgacagagtt ccagaaccgg gcgccatcga tgtgcagggg gacgccgccc
     1381 tcgtgcgcca ccgcagcgag actccggaca tacgccgacc cgacgggctg gttgttggcg
     1441 tgggcgctgg cgttctcgaa gactacgagg cccgtgatcg ggtcgtggac gtcggtcggg
     1501 tcgcgaaagg cctcgcggac ctgctggagg tcgagcgtcc cgtccggctg ctcatcgatg
     1561 gggcggaccg acgctccgac gaccacggcg tagctggccg cctcgtgcag catgatgtgg
     1621 ccgtgggtcc cggcgatgac ctcctggccg cgcccgacgt gcgccatctg cgcgacgagg
     1681 ttgccctgcg cgccggatgt gacgaagagc gcggcctcct tgccgagcag ctccgccgcg
     1741 cgctcctcga gcgcgttgac ggtcgggtcg tcgccgaaga cgtcgtcgcc gacttcggcg
     1801 tcggccatgg ccttgcgcat ctcgggcgtg ggcttggtga cggtgtcgga ccgcaggtcg
     1861 atgagggaca taggcccgcg aggatagcgc ggacggtctg ctatcctccg ccttcgtcgc
     1921 tcggacccgt ggtgtagcgg cccaacatgc cagcctgtca cgctggagat cgccggttcg
     1981 aatccggtcg ggtccgccac gctcctccga atccatccga cgccccgttc gcccgcccga
     2041 acggggcgtc atttcattcg gctgcgtcag tccgtggtcc gctggtcggc gggacccggc
     2101 cggtaccttc gtcgagtgac gaacagtggt gcgttcgcgc atccttcgtg gtgtgatcgg
     2161 ctcgatcggt tcgcgtcttc ggctcgttct tcgtcaaccg ggcctcgcga tgcgctccgc
     2221 cggtgccgtt gacggcctcg ccgaattccc cgagatcgac ttcgtcgcct attcgacgag
     2281 cgagcgcctg tccgggcgcg tccgcctcga cagcgcgcga ctcaccgaca tgctcaacgc
     2341 ccaccgtgag ttcgtcctcg tcgatgcgct cgccgagcgg ctcccggacg gcggctcgat
     2401 ggtcgtcccg gagatcctcg tcggccgcca cgagcttgcc ctcgtgcgcg tcggcggccc
     2461 gcgcggcgat cgcgccaagc gcgtcccgac gacgacgcac cgcctcgtga tccgcaccgg
     2521 ccgctacctc gtcgccggcc gcctccacag caacgccggc gaggatcccc tggcggcgct
     2581 ccgggcccgc gacccgatgg tcccgctcac cgacgccgcg atcatgttcc ggttcggacc
     2641 ggacatcgtc gaggaaccgg gcggcacgat cgtggtcaac cgcgacctcg tcgagtgggt
     2701 ccggcagggc gagctcgacg cacgcagcct ggacctgccc gcgaactgat ccgcgcccga
     2761 ctcccgcgcg cgagaattgg ccggtggcga ctctggttcc acctgatccc cgagtccgtg
     2821 cttcatttgc cgcggcgatg gacgagttcc gtgccgaggg gcggggtggc gcggacgacc
     2881 gatcggcgat tggccgatac ctgcgggagc actcgctcgc ctggttgtcc gatgcggcgt
     2941 tccgcgactt cacgggggcg atccgagccg agcagttcga ggacacgccg cggcccgctg
     3001 gattcgtgcc ggacacggag ctctggctgg tcgatggcaa ggcgtggctt ggccgcgtcg
     3061 ccgttcgcca ccggctgacg ccgaacctgc tggagatggg cgggcacatc ggctatgacg
     3121 tccggccgtc ggcgcgacgt cgcggtcacg cgaccgcgat gctccgggag gcgctcgtcg
     3181 tggcgcgcga cctcgggatc gggccggcgc tcgtgacctg cgacgtcggc aacgtcggct
     3241 cgcgcgccgt gatcgagcgc aacggcggtg tcctggagga cgagcgggcc ggcaagctgc
     3301 gcttctgggt gccgacggac tgactaccat ccgcgcgtgg caatcgagcc gaaccgaggt
     3361 cccctcgccg gcattcgcgt gatcgattgc tcgaccgtcc tggccgggcc ctactgcacg
     3421 atgctgctcg gcgacctcgg cgcgggcgtc gtcaagatcg agccgcccga gggcgacgcg
     3481 acccggggct ggggcccgcc gtgggtcggc tcggagtccg atgggtcgcg gacggctgcg
     3541 tactacctcg cggtcaaccg gaacaagcgg agcatccggc tcgacctgtc gaccgaggct
     3601 gggcgcgagg tcctgcgccg gctcctgcgc ggcggcgacg cggtcatcga gaactatcgg
     3661 gtcggtgggt tcgcgcgtct cggcttctcc gacgaggtcc tcgaggcgct gaatccgcac
     3721 ctcgtccacc tcgcgatcag cggcttcggc acgcgcgggc cggacgccgc gaagcccggc
     3781 tacgacttcg tgatccaggc ggtcggcggg ttgatgtcga tcacgggcga gcccgacggg
     3841 cggccgatga aggtcggcgt ggcgatcagc gacgtcgtga gcgggctctt cggcgcggtt
     3901 tcggtgatcg cgggcctgct ggcgcgcgag cgggacagca ctggcgatgg cagccgcgat
     3961 cacggtgagg gcggtcacgc gggcacgatc aaccgcgtgg gctcacggca acctgtcggt
     4021 cagcggatcg acctttcact cctccagagc acgctcgcgg tgctcgtcaa ccaggcccag
     4081 aacgcgcttg tcaccggagc ggcgccgaag cggcgcggca acgcccatcc cagcatcgtg
     4141 ccgtacgaga ccttcacgac cgccgacggc gagattgcga tcggggtcgg cagcgagcgc
     4201 cagtgggcgc gcctcgggcc ggcgatcggc ctgcccgaga tcgccaccga ggcgagattc
     4261 gccacgaatg ccgatcgcgt cgagcatcgc gacgagctca tcccgatcct cgccgcccgc
     4321 ttcgccacgg cgtcgaacga atcgtggctc acccgcctcg acgacgcggg cattcccgcc
     4381 gggccgatcc tcgacctgcc cgcggcgctc gagacgcccc aggcgcaggc actgggtagc
     4441 cgcgtccgca tggatcaccc ggtcctcggc cgtgtcgacc aggtcggcat cccgttcgag
     4501 ctgtcggcta ccccggcgtc gatccgcacc gcacccccga tgctcggcga gcacaccgac
     4561 gagatcctcc gcgaggctgg ctaccgccag ccggagatcg acgcgctgcg ggcggccggc
     4621 gtcgtttgat gagcctgggc ccggctcaca ccgcggcggc tggcgtcccg tgacgaggcc
     4681 cttcacggca cgcggaatgc cgagggcgtg atcacagggc gatcatttcc aggggttcgg
     4741 agcgcatttc ggacttgggc cagcggcatt cggcggtatc gggtcgcgga tcgcatttga
     4801 tcgaccgccg ccacgttcag attgagcgtg ccgctcgggc tcgcgccggg tttctggacg
     4861 gatcctgctg gctgggtgac cgaaaatggc tggcaacgtc cagaaaaaga tcgccccgtg
     4921 atcatgggac gacagttcgc atcgttgggc cgtcgccgcg gcttcagggt ctcgcagccc
     4981 ccttgctaga ctcgcggcga tccaatcgac cgctagctga gggccttcga cgatggccga
     5041 ttcgcgcgaa cgctgggcgc tgatcctggg aggctcgtcc ggcatgggcg aggcaacggc
     5101 tcgagcgctc gccgccgacg gctacaacat ctgcggtatc catctcgact ttcgggcctc
     5161 gctggaacgc gtcgagcggc tcaaggcaga ccttgtcgcg ctcggccgcg acgttctgta
     5221 catcaacatg aacgcggccg acgacgagaa gcgtgccgcg gcgctggccg agctcggcaa
     5281 gcggttcgag gccgcgcgcg cggccggtcg cgagccgtac gtgcgcgtgg tcatgcactc
     5341 gctcgccttc ggatcgctcg tcccgttcat ctcggaggac ccgaagggcg ccgtcgatcg
     5401 caagaagatg gagatgaccc aggacgtgat ggcgaacagc ctcgtctact gggtccagga
     5461 cctgtaccgc ggcggattcc tcgccgaggg ctcgaagatc tacgcgatga cctccgaggg
     5521 ctcgacccgg gtcgtgccgt catacggcgt cgtttcctcg gccaaggcgg cgctcgaatc
     5581 ccacatccgc cagctggcca tggagctcgg acgcctgaag tccggcatca ccgccaatgc
     5641 cattcgcgcc ggcgtgacga tgacgcccgc gctcatgaag atccccgagc acgacatgat
     5701 catcgcctcg gcgacggccc gaaaccccac cggccggatg acgacggtgc aggacgtcgc
     5761 gaacgcgatc gttgccctgt cgggcgaggg caccggcttc atcagcggcg aggtcatcgg
     5821 cgtcgacggc ggggaatacg tcaccggctc gtgagcgaac ccggcgccgg ctcgatccgg
     5881 ccgttctatg ccgactgggc gggctataac cgtcggatca tcgacgcagt cgcgcggatg
     5941 acctccgacg acctcgcgct ccaggcgccg ggcacggagc actggccgat ctgggcgatc
     6001 tgtgggcaca tggccggcac gcgggtcttc tggctatgcg atgttttcgg ggaggccggt
     6061 atcgagcaga cgccttggcc gaacgggacg ctcggccccg gctgggagga cgacctctcg
     6121 gtgccgcgca gcgccgagga gctcgtctgg gcgctcacct cgacctggca ggtcatcgag
     6181 ggtctcctcg ggcgctggac acccgactcg atggatatga ccgcgcagcg caacaaggcc
     6241 gggagagcgc cggagatcca cacccgccag tcgatcctgc tgcgcatgat cacccacgag
     6301 ggctaccacg tcggcgagat caacatcgcg ctcggcgcgg ccggccgcga gcagatcgac
     6361 ccgtggcccg ggcgcgagtg gcaggcagac gcgccgcgct cgctacgtga gggtggcagc
     6421 taggcaaccg cctccccgcg gacccagctc gtctgttggc gcgccgccgg cgtcatcgag
     6481 agttgaccgg attccgagac ccaccacgcc gccagcccac gtcgccgcgc agctgcgccg
     6541 attgcatctg cgccc
//
LOCUS       DATBSW010000165         7670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_508858, whole genome shotgun sequence.
ACCESSION   DATBSW010000165 DATBSW010000000
VERSION     DATBSW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508858"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VIF63_04625"
     CDS             complement(<1..1369)
                     /locus_tag="VIF63_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8238699.1"
                     /translation="MRAIPLARAARRKHGLEPIVGRDGTLDAADAGAARRIAARLTAA
                     RDAAGQPAVSAGEVAALAALDAALHRLIDTERTSGRADLGKAVEAVDSALGAGALAEV
                     EEAWRGQFRDGNGGNGGGRRRTKPAPGAELLAEMLVLNALNENAAATDVRELVDDRPL
                     RDETRYEAVVQATEQELGGSADRGPAERGGRGGRRGVANASTGPDPLPARLREPMRRA
                     PGSLAAQLRWVREHWTALVEGDPDLARRIDLALDVLAEEARALELKGAGDHFGGPIVV
                     ETPDYRGLDSDAVGFSYDTDWMPSVVLMAKSTYVWLEQLSKTFGRNISTLAAVPDEAL
                     DRLAHLGVTGLWLIGLWQRSRASAEIKRRRGDGEAVASAYAIDEYRIADDLGGEPAFE
                     DLKARAWARGIRIAADMVPNHMGIDSRWVVEHPERFVATQEPPFPAYSFGGPDLSGDD
                     RAEITL"
     gene            complement(1383..2441)
                     /locus_tag="VIF63_04630"
     CDS             complement(1383..2441)
                     /locus_tag="VIF63_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8238700.1"
                     /translation="MSESGPGPSFSGLERGLDVERDSDVPISTQIYWQLAYQIESGRL
                     QSGERLSPVRELGAALRVNPNTIRAVYRRLADNGYVISRHGAGTVVSDRSVHRRRPEA
                     LGGIVSEMLRRAAQAGFTPDEVAAAAYAVASERKRPGKQVRVLFAECTTADAGYDAER
                     LNREFAGAIEAEGTLLDEIPERLERFHYDLVATTTFHADEAQALVRGRVPVVAMLVGP
                     GYVELVHEISALPEGSRVGVICASDRGTDNIAETLSIAGTKGVEIVSATIQDDERLTL
                     VDRTADIILMSREALAQKLDQRLHRPERIRPWTYEFDPAGLELLRRSVEHVAAVRPKA
                     GRAEADRKETGRPETAKV"
     gene            2525..2656
                     /locus_tag="VIF63_04635"
     CDS             2525..2656
                     /locus_tag="VIF63_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238701.1"
                     /translation="MSGLVLIFAAFVVLAALDLIAASNGVDSRQDFDDVHAPVRGLR"
     gene            complement(2726..4933)
                     /locus_tag="VIF63_04640"
     CDS             complement(2726..4933)
                     /locus_tag="VIF63_04640"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEY8238702.1"
                     /translation="MTRRISLALTLHNHQPIGNFGWVFAEVFEQAYQPMVAALLRHPT
                     VRVGLHYTGPLLEWLIAERPAFIADLRALVEREQVEILGGGLYEPVLASLPERDRVGQ
                     LSRMADAVEAAFGRRPHGAWLAERVWEPDVPTSLAAAGYAWTILDDSHFRAAAVPEDQ
                     MWGPYTTEDQGRLISVFGTEQGLRYRIPFQEVDEVIDYLRDHATEGGERVGTMGDDGE
                     KFGAWPTTWEHCWGKGRWVERFFEAIEANGAWLSTVRPSDWLASSAPVGRVYLPTGSY
                     AEMGEWALPPVEARGFSAALHRARDAGAPEARWLRGAFWRNFQVRYREINDLHKQMLR
                     VSGEVEGMVPGRERDSAMDHLYRGQSNDCYWHGLFGGIYIAHMRAATLSHLIAAEDIA
                     DAALGTTVRSEVRDLDLDGRDEILLASDGQVVTIDLDEGAGIGAWDLRAARHALTGVL
                     RRRPEAYHETLREHEAKAVAAANAPKGAGASEGAGAASIHDRVQVKEAGLAARLIYDD
                     YERRSGLLRVLPADATAASWGAGGRDEAADLATGQFRLVRLATDEAVVARDGTVTLGD
                     RSWPVSLETRIQLGGGRLDPTLDWTATLTNAGNAPLVARVGVEWAITMLGGGGNPEAW
                     WELDGARARHDGSAAATGVERVAQGNGWLGVELESAAEPAADAWHAPIETVSNSEAGF
                     ERVYQGSALLLSWPVRIEPGERWSVTIRHRASIAVDRAVEEAESARSTLVAGT"
     gene            complement(4930..7110)
                     /gene="glgP"
                     /locus_tag="VIF63_04645"
     CDS             complement(4930..7110)
                     /gene="glgP"
                     /locus_tag="VIF63_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY8238703.1"
                     /translation="MEPTVRVPSAAPIHVRVPPELEGLRRLAYNLYWSWHPKARGLFA
                     RINSGTWARTHSPIPVLQGMVDWAALLDNPDFMAEAYRVVGDFDRYMANGADHWFHRK
                     HARELKGPIAYFCAEYGLHESLGIYSGGLGVLAGDHMKAASDMALPLVGVGLLYRNGY
                     FRQTIDADGHQEHRYPDYDLARLPLTRALGREGDPLTVSVELPGRDVKVDVWTVQVGR
                     VPVLLLDTDNPVNDAADRPITNILYVRGREMRLHQELILGIGGVRALRGLGIEPAAWH
                     LNEGHSAFLLAERARELVAAGQSLEDALATISRNSVFTIHTPVSAGNERFDTDLVRRI
                     AGPILDGDGRPNTGGVPVERALELGRGADGDHGQFDMTAFSLRLTTGANAVSKLHAVT
                     ANATWEGIAPNGIKAITNGVHPPTWVGGPIRDLFARFLDADLDDIDGSTNASRWWERI
                     ERIPATELWEAHVRQKRELAVFARGRLRTQFARHGEAPSTLRQLGEAFNPKALTIGFA
                     RRFATYKRAGMIFTDTDRLARLLSDADRPVQLVFAGKAHPADRPGQSVIQEIFGRTRA
                     PEFRGRVFILEDYDMRIGRFLVQGVDVWLNNPRRPLEASGTSGMKAAMNGIPNLSVLD
                     GWWDEGFQGDNGWAIGGRETLADEAAQDWNDAMDLYRILESETVPEYYERDARGLPAR
                     WVERMRRAMATSLWQFSTTRMLHEYTEQQYLPAAGIEVGRAVPS"
     gene            7202..>7670
                     /locus_tag="VIF63_04650"
     CDS             7202..>7670
                     /locus_tag="VIF63_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY8238704.1"
                     /translation="MAIRVAFIAAECEPWAKTGGLADVVDALARALGQVGGSELDGLV
                     DVYLPNYRGVPVPAGAQSVAVSVPDPSAGGAPVEVRILIAETHGYRLHLVDHPPAFDR
                     EGMYGTPTADHPDNAWRFGLLCRAALEHLRAAARPPDVIHIHDWHTVPALLLRD"
BASE COUNT         1058 a         2756 c         2701 g         1155 t
ORIGIN      
        1 cgagggtgat ctccgcccgg tcgtcgcccg acaggtccgg gccgccgaac gagtaggcgg
       61 ggaacggcgg ctcctgggtc gccacgaagc gctcggggtg ctcgacgacc cagcgcgagt
      121 cgatgcccat gtggttgggg accatgtccg ccgcgatccg gatgccccgc gcccaggcac
      181 gtgccttgag gtcttcgaac gccggctcgc cgcccaggtc gtcggcgatt cggtattcgt
      241 cgatggcgta ggccgaggcg acggcctcgc catcgccgcg ccgacgcttg atctcggcgg
      301 atgcccggct gcgctgccac agcccgatca gccacagccc cgtcacgccc aggtgcgcca
      361 ggcgatcgag ggcctcgtcg ggcacggccg cgagggtcga gatgtttcgg ccgaaggtct
      421 tcgacagctg ctcgagccag acgtacgtcg acttcgccat cagcacgacg ctcggcatcc
      481 agtcggtgtc gtacgagaag ccgacggcgt ccgaatcgag gccgcggtag tcgggcgtct
      541 cgacgacgat cgggccgccg aagtggtcgc cggcgccctt cagctcgagg gcgcgcgcct
      601 cctcggcgag gacgtcgagg gcaaggtcga ttcggcgcgc gaggtccggg tcgccctcga
      661 ccagggcggt ccagtgctcg cggacccagc gcagctgcgc cgcgagcgag cccggcgcgc
      721 gccgcatcgg ctcgcgcagg cgtgccggca ggggatcggg cccggtcgac gcgttggcca
      781 cgccgcgccg cccgccgcgc ccaccgcgct ccgccgggcc gcggtcggcg gagccaccga
      841 gctcctgctc cgtcgcctgt accacggcct cgtaccgcgt ctcgtcgcgc agcggccggt
      901 cgtcgacgag ctcgcgcacg tcggtcgcgg ccgcgttctc gttcagggca ttgaggacga
      961 gcatctcggc aaggagctcc gcgcccggcg ccggtttcgt gcgccgccgc ccgccgccgt
     1021 tgccgccgtt cccgtcccgg aactgcccgc gccacgcttc ctcgacttcg gccagcgcgc
     1081 ctgccccgag cgccgaatcc accgcctcga ccgccttgcc gagatcagcc cggcccgatg
     1141 tccgctcggt gtcgattaac cggtggaggg ctgcgtccag cgcggcgagc gccgcaacct
     1201 cgcccgcgct caccgccggc tgaccggcgg cgtcgcgtgc tgcggtcagg cgggcggcaa
     1261 tccggcgagc agcgcccgcg tcggcggcgt cgagcgtgcc gtcccggccc acgatgggtt
     1321 cgaggccgtg cttccggcgt gccgcgcgtg ccagggggat cgcccgcatc gggcgaagga
     1381 tgtcagacct tggccgtttc gggacgaccg gtctccttgc gatctgcctc ggctcggccg
     1441 gccttcggcc gaacggcggc gacgtgctcg accgagcggc gcagcagctc gagcccggcg
     1501 gggtcgaact cgtacgtcca gggccggatc cgctcgggcc ggtggaggcg ctggtcgagc
     1561 ttctgggcga gggcttcgcg cgacatcagg atgatgtcgg cggtccgatc gacgagggtg
     1621 agccgctcgt cgtcctggat cgtcgccgac acgatctcga cgcccttggt gcccgcgatc
     1681 gagagcgtct cggcgatgtt gtcggtgccc cggtcggacg cgcagatcac gccgacgcgc
     1741 gagccctcgg gcagcgccga gatctcgtgg acgagctcca cgtagcccgg cccgacgagc
     1801 atcgcgacga ccggcacgcg gccgcggacc agcgcctggg cctcgtcggc gtggaacgtc
     1861 gtcgtcgcga cgaggtcgta gtggaagcgc tcgaggcgct cggggatctc gtcgagcagg
     1921 gtgccctcgg cctcgatggc gccggcgaac tcgcggttca ggcgctcggc gtcgtagccg
     1981 gcgtcggcgg tggtgcattc ggcgaagagg acgcggacct gcttacccgg tcgcttccgc
     2041 tccgacgcca ccgcgtatgc ggcggcggca acctcatcgg gcgtgaaacc ggcctgagcg
     2101 gcgcggcgaa gcatctcgga gacgatcccg cccaacgctt cggggcgacg gcgatggacg
     2161 cttcgatcgg agacgacggt gcccgcgccg tgccggctga tcacgtagcc gttgtcggcc
     2221 agtcggcggt agaccgcgcg aatcgtgttc ggattgaccc gcagggccgc gcccagctcg
     2281 cgtaccggcg acaggcgctc gcccgactgg agccgcccgg attcgatctg gtaggcgagc
     2341 tgccagtaga tctgggtcga gatcgggacg tccgaatcgc gctcgacatc gaggccgcgc
     2401 tcgaggcctg aaaacgatgg tccggggccc gattcactca tttgacatag tccactagtc
     2461 caagtatcgt tcgacttggc ctagtgaact gtaccaaatc gctccacaga aaggatggtc
     2521 acaaatgagt ggcctggtct tgatcttcgc ggcgttcgtc gtgctcgcgg cgctcgacct
     2581 cattgcggcg agcaatggcg tcgacagccg acaggacttc gacgacgtgc acgcacctgt
     2641 ccgagggctc cgctgagcct tcgatcctcc gacgcatccc tcccctcgtc agcccgtggc
     2701 gcgcaggccg cgggctgacg gattctcagg tccctgcgac cagtgtcgac cgcgctgatt
     2761 cggcttcctc cacggctcgg tcgaccgcaa tgctggcgcg gtgccggatc gtgaccgacc
     2821 agcgctcccc gggctcgatc ctgaccggcc acgacaggag cagcgcgctg ccctggtaga
     2881 cacgttcgaa gccggcctcc gagttcgaga ccgtctcgat cggggcgtgc caggcatccg
     2941 ccgcaggctc cgcggcggac tccagctcga caccgagcca gccgttcccc tgcgcaaccc
     3001 gttcgacacc cgtggctgcg gccgaaccgt cgtgccgcgc gcgcgctccg tcgagctccc
     3061 accatgcctc cgggttgccg ccaccgccca gcatcgtgat cgcccactcc accccgacac
     3121 gcgcgacgag cggcgcgttg ccggcgttgg tgagcgttgc cgtccagtcg agcgtcggat
     3181 ccagccgacc gccgcccagt tggatccggg tctccaggga taccggccag ctccgatcgc
     3241 cgagcgtgac cgtgccatcc cgcgcgacca cggcttcatc cgtcgccagc ctgaccagcc
     3301 ggaactggcc ggtggcaaga tcggcggctt cgtcgcgccc gccggcgccc cacgacgcgg
     3361 ccgtcgcatc agccggcagg actcggagca ggcccgaccg gcgctcgtag tcgtcataga
     3421 tgaggcgcgc cgcgaggccg gcttccttta cctgcacccg gtcgtggatc gacgccgcgc
     3481 ccgcgccctc gcttgcaccg gcgcccttcg gcgcgttcgc tgcggcgacc gccttggcct
     3541 cgtgctcgcg cagggtctcg tgatacgcct cgggccggcg tcggagcacg cccgtgagcg
     3601 cgtgccgcgc cgcccgcagg tcccacgcgc cgatgcccgc accctcgtcg aggtcgatcg
     3661 tgacgacctg gccgtcgctc gccaggagga tctcgtcgcg gccatcgagg tcgaggtcgc
     3721 gaacctcgct tctgaccgtc gtgcccagtg ccgcgtcggc gatgtcctcc gcggcgatca
     3781 ggtgggacag cgttgccgcg cgcatatggg cgatgtagat cccgccgaag aggccgtgcc
     3841 agtagcagtc gttcgactgg ccccggtaaa ggtggtccat cgccgagtcc cgctcccgac
     3901 ccggcaccat cccctcgacc tcgcccgaga cccggagcat ctgcttgtgg aggtcgttga
     3961 tctcgcggta ccggacctgg aagttgcgcc agaacgcgcc gcgcagccag cgtgcctcgg
     4021 gtgcgcccgc gtcgcgagcg cggtgcaggg ccgccgagaa cccgcgcgcc tcgaccggcg
     4081 gcaaggccca ttcacccatc tcggcatatg acccggtcgg gagatagacc cggccgaccg
     4141 gcgcgctcga cgccagccag tcgctcgggc ggaccgtcga cagccacgcg ccgttggcct
     4201 cgatcgcctc gaagaatcgc tcgacccagc ggcccttgcc ccagcagtgc tcccacgtcg
     4261 tcggccaggc gccgaacttc tcgccgtcgt cgcccatcgt gccgacgcgc tcgccgcctt
     4321 cggtcgcatg gtcgcgcagg tagtcgatga cctcgtcgac ctcctggaac gggattcgat
     4381 agcgcaggcc ctgctcggtc ccgaagacgc tgatcaggcg gccctggtcc tcggtcgtgt
     4441 acgggcccca catctggtcc tcgggcaccg cggcggcacg gaaatgcgaa tcgtcgagga
     4501 tcgtccaggc gtatcccgcg gccgcgagcg acgtcgggac gtcgggctcc cagacgcgct
     4561 cggccagcca cgcgccgtgc ggtcggcgcc cgaacgccgc ctccacggcg tcggccatcc
     4621 ggctcagctg gccgacccgg tcgcgctccg gcagcgacgc caggaccggc tcgtacaggc
     4681 cgccgcccag gatctccacc tgctcacgct cgacgagggc gcgcagatcc gcgatgaacg
     4741 ctggtcgctc ggcgatcagc cactcgagca gaggcccggt gtaatggagg ccgacgcgca
     4801 ccgtcgggtg gcgcaggagc gccgcgacca tcggctggta ggcctgttcg aagacctcgg
     4861 cgaagaccca gccgaagttg ccgatcggct gatggttgtg cagcgtcagc gcaagggaga
     4921 tgcgccgggt catgacggga cggcccggcc gacctcgatg ccggcagccg ggaggtactg
     4981 ctgctccgtg tactcgtgca gcatccgcgt ggtcgagaac tgccacaggc tcgtggccat
     5041 cgcgcggcgc atccgctcga cccagcgcgc tggcaggccc ctggcgtcgc gctcgtagta
     5101 ctcgggcacc gtctcggact cgaggatccg gtacaggtcc atggcgtcgt tccagtcctg
     5161 ggccgcctca tcggcgaggg tttcgcggcc accgatcgcc cagccgttgt cgccctggaa
     5221 gccctcgtcc caccacccgt cgagcacgct gaggttcggg atcccgttca tcgccgcctt
     5281 catgcccgag gtacccgacg cctcgagcgg tcgccgtggg ttgttcagcc agacatcgac
     5341 accctggacg aggaaccgcc cgatgcgcat gtcgtagtcc tcgaggatga acacccgacc
     5401 gcggaactcc ggcgcacgcg ttcggccgaa gatctcctgg atcacggact ggcccgggcg
     5461 gtccgccgga tgggccttgc cggcgaagac cagctgaacg ggccggtcgg cgtcgctcag
     5521 caggcgagca agccgatccg tgtcggtgaa gatcatcccg gcgcgcttgt acgtggcaaa
     5581 ccggcgagca aagccgatcg tgagggcctt gggattgaac gcctccccga gctgccggag
     5641 cgtgctcggc gcctcgccgt ggcgagcgaa ctgggtgcgc aggcgcccgc gggcgaagac
     5701 cgcgagctcg cgcttctggc ggacgtgcgc ctcccacagc tcggtcgcag ggatgcgctc
     5761 gatgcgctcc caccaccggc tcgcgttggt cgacccgtcg atgtcgtcga gatccgcatc
     5821 gaggaagcgc gcgaagaggt cgcggatcgg gccgccgacc cacgtcggcg ggtgcacacc
     5881 gttcgtgatc gccttgatcc cgttcggggc gatgccctcc cacgtggcat tggcggtgac
     5941 cgcgtggagc ttgctcactg cgttggcacc ggtcgtgagc cgcaggctga acgcggtcat
     6001 gtcgaattgg ccgtggtcgc catcggcgcc gcgcccaagc tcgagcgccc gctcgaccgg
     6061 gacgccgccg gtgttcgggc gcccgtcgcc gtcgaggatc gggccggcga tgcgccggac
     6121 gaggtcggta tcgaagcgct cgttgccggc cgagaccggc gtgtggatcg tgaacacgct
     6181 gttccgcgag atggtcgcca gggcgtcctc gagcgattgg ccggcggcga caagctcgcg
     6241 ggcgcgctcg gcgagcagga atgccgagtg gccttcgttc aggtgccacg ccgcgggctc
     6301 gatgcccagg ccgcgcagcg cgcgcacgcc gccgatgccc aggatcagct cctggtggag
     6361 ccgcatttcg cggccgcgga cgtacaggat gttcgtgatc ggccggtcgg ctgcgtcgtt
     6421 gaccgggttg tcggtgtcga gcaggagcac cggcacccgc ccgacctgga ccgtccagac
     6481 atcgaccttc acgtcgcgtc cgggcagctc gaccgacacg gtcagcgggt cgccctcgcg
     6541 cccgagggca cgggtcaacg gcagccgggc gagatcgtag tcggggtagc ggtgctcctg
     6601 gtggccgtcg gcatcgatcg tctggcggaa gtagccgttt cgatacagga ggccgacgcc
     6661 gacgagcggc agggccatgt cgctcgccgc cttcatgtgg tcgccggcga gcacgccgag
     6721 gccgccggag tagatgccga gcgactcgtg caggccgtac tcggcgcaga agtaggcgat
     6781 cgggcccttg agctcgcggg cgtgcttgcg gtggaaccag tggtcggcgc cgttcgccat
     6841 gtaccggtcg aagtcgccca cgactcggta cgcctcggcc atgaaatcgg ggttgtcgag
     6901 gagcgcagcc cagtcgacca tgccctggag gacggggatc gggctgtggg ttcgggccca
     6961 ggtgccgctg ttgatccggg cgaacaggcc gcgtgccttg ggatgccagg accagtacag
     7021 gttgtaggcg agacgccgca agccctccag ctccggcgga acgcggacgt gaatcggcgc
     7081 ggccgaaggg actcggacgg tgggctccat ccgcgggatc atatcggcga accggaacgc
     7141 gtgtaaaccg gtttagcaag ggcgccgccg gcgcgcgttg cgcccgcgcc atactccgct
     7201 cgtggcgatc cgcgtggcgt tcatcgcggc cgaatgcgag ccttgggcga agaccggtgg
     7261 cctggccgac gtggtcgatg cgctggctcg cgcgctgggc caggtcggcg ggtcggagct
     7321 cgacgggctc gtcgacgtat acctgccgaa ctaccggggc gtaccggtgc cggccggtgc
     7381 ccaatccgtc gcggtttccg tgcccgatcc gtcggcaggc ggcgccccgg tcgaggtgcg
     7441 gatcctgatc gccgagacgc acggctatcg gctgcacctg gtcgaccacc cgcccgcatt
     7501 cgatcgcgag ggcatgtacg gcacgccgac ggccgaccac ccggacaacg cgtggcggtt
     7561 cgggctcctg tgccgcgccg cgctcgagca ccttcgcgcc gccgcgcggc cgccggacgt
     7621 cattcacatc cacgactggc acaccgtgcc ggcgctgctc ctccgcgacg
//
LOCUS       DATBSW010000166         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1249163, whole genome shotgun sequence.
ACCESSION   DATBSW010000166 DATBSW010000000
VERSION     DATBSW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249163"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..972
                     /locus_tag="VIF63_04655"
     CDS             265..972
                     /locus_tag="VIF63_04655"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY8238705.1"
                     /translation="MPTAPSKLAIVVRGGWDGHAPIEATDLFIPHLEANGYEVRIFDS
                     PAPYADPDLMARTDLIMQCNTMATIARDELAGLRAAIANGTGMAGWHGGIADSYRNSS
                     DYYQLIGAQFACHPGKDPATITGDGPENNYVPYTVNLLPAAADHPITRGLADFDLVTE
                     QYWVLWDRYLDVLATTTQKVRPWDEWHREITSPAIWTRRWGKGRIFVCTPGHSLDVLA
                     HPTVHTVIERGLLWAGR"
     gene            981..2072
                     /locus_tag="VIF63_04660"
     CDS             981..2072
                     /locus_tag="VIF63_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8238706.1"
                     /translation="MIGCGAIARQYLDTARRLDAIEIVAAADRVPARATAVEAAEGIP
                     AMPVADLLANPEVDVVLNLTIPAAHAAIALQAIAAGKAVYGEKPLAATTAEARSILEA
                     ARAAGVQVGCAPDTVLGTGIQTARKAVDDGRIGAPIAATATMVTPGHERWHPDPDFYY
                     QPGGGPLLDMGPYYVTALVTILGPVVAAIGAASRTRGTRTIRSGARAGETVPVAVDSH
                     VTGALIHESGAISTLVMSFDAVASRASRIEIHGELGSLIAPDPNLFRGDVELRELDGT
                     GWVTLPISAGYERSSRGYGIADLAATPAGEEPRAGGALAFHVLDVMESLLESARTGRR
                     VDVASRCPRPAPVPLQSLPSSDAEIPTYH"
BASE COUNT          316 a          844 c          775 g          305 t
ORIGIN      
        1 gccaggtagc gttggtgaat gcgcaacctt ggcgccctgc tggtcctcgt cgtgtacttc
       61 ggggtcttcg gctgggttgc ctcgttcccg tccgcctgat cgacgcggcg gcgagcgccg
      121 ccgcggaagc cggagccgtg atagaactcg gacgatgtcc gccagggctt tcgcccggga
      181 tcacgcggcg cccgcgccgc ccggctcgga cccgcggatc gcgtcgcccg cccacccacc
      241 gttagaacgg agaccgcgtc ccgcatgccg actgccccat cgaagctcgc catcgtcgtc
      301 cgcgggggct gggacggcca cgcgcccatc gaggcgacgg acctgttcat cccgcacctc
      361 gaggcgaacg gctacgaggt ccggatcttc gactcgccgg cgccgtacgc cgatccggac
      421 ctgatggcgc ggaccgacct gatcatgcag tgcaacacca tggcgacgat cgcccgcgac
      481 gagcttgcgg gcctgcgagc ggcgatcgcc aacgggaccg gcatggcggg ctggcatggc
      541 gggatcgccg attcgtatcg caactcgtcc gactactacc agctcatcgg cgcccaattc
      601 gcttgccatc cgggcaagga cccggcgacg atcaccggcg acggacccga gaacaactac
      661 gtgccgtaca cggtcaacct gctgccggcc gcggccgacc accccatcac ccggggcctc
      721 gcggacttcg acctggtcac cgagcagtac tgggtgctct gggaccgcta tctcgacgtc
      781 ctcgcgacga cgacccagaa ggtccggccg tgggatgagt ggcaccgcga gatcacctcg
      841 cccgccatct ggacgcggcg ctggggcaag ggccggatct tcgtctgcac ccccggccat
      901 agcctcgacg tcctcgccca tccgaccgtc cacaccgtca tcgagcgggg cctgctgtgg
      961 gccggccggt gaggatcggg atgatcggtt gcggcgcgat cgcccggcag tacctcgaca
     1021 ccgcccgccg gctcgacgcg atcgagatcg tggcggcggc ggatcgcgtc ccggcccgcg
     1081 ccaccgcggt cgaggcggcc gagggcatcc ccgcgatgcc ggtcgccgac ctcctcgcga
     1141 atcccgaggt ggatgtcgtc ctgaacctca cgatcccggc cgcccacgcc gccatcgccc
     1201 tccaggcgat tgccgcgggc aaggcggtct acggcgagaa gccgctggca gcgacgacag
     1261 cggaggcgcg ctcgatcctc gaggctgccc gggcggccgg cgtgcaggtc ggctgcgcgc
     1321 ccgacacggt cctgggaacg gggatccaga cggcgcggaa ggccgtcgac gacggacgga
     1381 tcggggcccc gatcgcggcg accgcgacca tggtcacgcc cggccatgag cgctggcacc
     1441 ccgatcccga cttctactac cagcccggcg gcggcccgct tctggacatg gggccgtact
     1501 acgtgaccgc cctcgtcacg atcctcggcc cggtcgtcgc cgccatcggc gccgcgagcc
     1561 gcacgcgcgg gacgcggacg atccgctcgg gcgcgcgcgc cggcgagacg gtcccggtcg
     1621 ccgtcgacag ccacgtgacg ggcgccctga tccacgagtc gggcgcgatc tcgacgctcg
     1681 tcatgagctt cgacgcggtc gcctccaggg cgtcgcggat cgagatccac ggggagctgg
     1741 ggtcgctcat cgcgccggac ccgaacctgt tccgcggcga cgtcgagctg cgcgagctcg
     1801 acgggaccgg ctgggtcacg ctcccgatca gcgccgggta cgagcgcagc tcgcgcggct
     1861 acgggatcgc ggacctggcg gcgacccccg ccggagagga gccgcgcgcc ggtggcgccc
     1921 tcgcgttcca cgtgctggac gtcatggaat cgctcctgga gtcggcccgg accggtcgcc
     1981 gggtcgacgt cgcgagccgc tgcccgcggc cggcgccggt gccgctccag tcgctgccga
     2041 gctcggacgc ggagatcccg acctaccact agccggaccg cgttcgcggc gatgaccgcg
     2101 gctcttgccg tggcgagcgt cggcaccctg atgaaggtcg atctagtctc ggccgaacgg
     2161 ccctcgcgaa cgcgcccttc ggcactcccg cgcgacagcc ccccacccta cggttcgatg
     2221 ggaggcagcc gatggcgatc
//
LOCUS       DATBSW010000167         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1179123, whole genome shotgun sequence.
ACCESSION   DATBSW010000167 DATBSW010000000
VERSION     DATBSW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179123"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VIF63_04665"
     CDS             <1..247
                     /locus_tag="VIF63_04665"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEY8238707.1"
                     /translation="TARGRDRLRSNQFAYVDRDGSEHLPIHDEAHVRNAIARFNQVDF
                     ESAAAKERARKKVLAAAERHGVEVSDDSNVARRGRSG"
     gene            489..710
                     /locus_tag="VIF63_04670"
     CDS             489..710
                     /locus_tag="VIF63_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238708.1"
                     /translation="MQRLLQVVLSAWREAERVAAERSETSREHGAALIAADRLRALYG
                     ELLDSAKADADTAVVQAAPPLDTLESGGR"
     gene            830..1996
                     /locus_tag="VIF63_04675"
     CDS             830..1996
                     /locus_tag="VIF63_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8238709.1"
                     /translation="MAAIGLTNLPLDLPTATEVFPAVRFESPSIESPTHRQFLEALGV
                     AVYTTDSEGRITFYNEAAAAFWGRRPELGELWCGSWRLFWPDGTPLAHDECPMAIALR
                     EGRSVRGSEAVAERPDGTRVAFIPYPTVVEDADGRVIGAVNVLVDVSRQYRAEEAAHA
                     ARAAKDEFLAMVSHELRTPVTTIFGNARLLRERGSLPDGPAAMIADIETDAERLLGVV
                     ENLLTLTSGTAARQVDREPQILGRVLSRVVASFERRHPGRSVPLAIQDPHAIVEADEG
                     YLELLIGNLLSNAHKYSPIAAPIDVGLRQVDGEVEVTFLDRGIGIGPEDVEVLFESFY
                     RAEAARKISGGLGIGLSACKLIVQLLGGRIWARPRPDGGSEFGFALPLSPDPSD"
     gene            complement(2050..3123)
                     /locus_tag="VIF63_04680"
     CDS             complement(2050..3123)
                     /locus_tag="VIF63_04680"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY8238710.1"
                     /translation="MATFPDPLGSVGWTPARPVLTVVGCHAGGEIGNVVVAGVEPPPG
                     ATVFEQMQAIAADDSIRKLLLHEPRGSVAVHSNIVLPALREDCAFGYVIMEPTEYPAM
                     SGSNTICVATVLLETGMVEMHEPTTTFRLEAPGGPIDITAGCRAGKVEWVELENVPCF
                     ADRLDAPLEVEGLGTIKVDVAFGGMWYAIADAAALGFALEPSEAADLSRMGTRIHKAA
                     REQLHPVHPENPEIREVSVIELAEPWRGNGRISKNAVIVAPGRLDRSATGTGLSARIA
                     TLHARGRMHVGHSMTHASVIGSTFDGRIVREVEIGGRTGIVPAIRGTAWITGVTHVLL
                     DPTDPFPEGYLLTDTWPGSDVQA"
BASE COUNT          524 a         1158 c         1174 g          516 t
ORIGIN      
        1 gactgcgcgc ggtcgcgatc gactgcggtc caatcagttc gcgtacgtgg atcgcgacgg
       61 cagcgagcac ctgccgatcc acgacgaggc ccatgtccgc aacgcgatcg ctcgcttcaa
      121 ccaggtggac ttcgagagcg cggccgccaa ggagcgagcg cggaagaagg tcctcgccgc
      181 ggccgagcgc cacggcgtcg aagtgagcga cgactcgaac gtcgcgcggc ggggtcgctc
      241 cggctagtcc gaagcgcctt cgcgccgctg gcgggcgagc tcgagcgaat ttgcaccaga
      301 cccctaacgc gatacctggc aattcgttat tgacggttgc aggccccgag gatgcgacgt
      361 cgttcccccg gcgacggacg tgaccccggg gctgcttctt gcccggcgcc cgatcaattt
      421 gatttccatg gcgcctcgag gcatcgggac aggtatgctc ccgcacctct acgacagagg
      481 ggtcggcggt gcagcgattg ctccaggtag tcctgagcgc ctggcgcgag gctgaacgcg
      541 tcgccgccga gcgatccgag acgtcgcgcg agcatggcgc tgccctcatt gccgccgacc
      601 ggttgcgggc gctgtacggc gagctcctcg attcggcgaa ggcagacgcg gatacggccg
      661 tggtccaggc ggcgccgccg ctcgataccc tggagtcggg cggccgctga aactccgcgg
      721 ggcaacggtc gcccgcgccg ttgcaacttc cagacaaccg gcccgcatgg gtggccctcc
      781 gcgcagggcg tgtggtatga gaaggccatg atccggaccc acgcctgaaa tggcggcgat
      841 cggcctgacg aaccttcccc tcgatttgcc aactgcgacc gaggtcttcc cggcagtccg
      901 cttcgagtcg ccatcgatcg agagtccgac ccaccgccag ttcctcgagg cgctcggcgt
      961 cgccgtgtac acgacggata gcgagggccg catcaccttc tacaacgagg cggccgccgc
     1021 cttctggggg cggcgcccgg agctgggtga gctgtggtgt ggatcgtggc gcctgttctg
     1081 gccggacggc acgccgctcg cccacgacga atgcccgatg gcgatcgcac tgcgtgaggg
     1141 ccggtcggtc cgcgggtccg aggccgtcgc ggaacggccg gatggcacgc gcgtggcgtt
     1201 catcccgtat ccgacggtcg tcgaggacgc cgatgggcgc gtgatcggcg cggtgaacgt
     1261 gctcgtcgac gtcagccgcc agtaccgggc agaggaagcg gcgcacgccg cgcgggcggc
     1321 caaggatgag ttcctggcca tggtctcgca cgagcttcga acgcccgtca cgacgatctt
     1381 cgggaacgcg cgactccttc gggagcgtgg ctccctgccc gacgggcctg cggccatgat
     1441 cgcggacatc gagaccgacg ccgagcgcct gctcggggtg gtcgagaacc tgctgaccct
     1501 gaccagcggc acggccgcca ggcaggtcga ccgggagcca cagatcctcg gacgcgtcct
     1561 gtcgcgcgtc gttgcctcgt tcgaacgccg gcaccccggt cgctcggtcc cgctcgcaat
     1621 ccaggatccg cacgccatcg tcgaggcgga cgagggctat ctcgagctgc tcatcggcaa
     1681 tctcctgagc aacgcccaca agtacagccc gatcgcagca ccgatcgatg tcgggctgcg
     1741 ccaggtcgac ggcgaggtcg aggtcacctt cctggaccgc ggtatcggca tcggccccga
     1801 ggacgtcgag gtcctcttcg agagcttcta tcgcgccgag gcggcccgga agatcagcgg
     1861 cggcctgggt atcgggctca gcgcgtgcaa gctcatcgtc cagctcctcg gcggccggat
     1921 ctgggcccgc ccgcgacccg atggcgggtc cgagttcggg ttcgccctgc cgctgagtcc
     1981 cgacccgagc gactgacgga ccgaccccag ccgtcaaagc ggtgcgtccg cttgaggctc
     2041 gctcgcgggt caggcctgga cgtcactgcc gggccaggtg tcggtcagca ggtagccctc
     2101 ggggaaggga tcggtggggt cgaggaggac gtgggtcacg ccggtgatcc aggcggtacc
     2161 ccggatcgcg ggcacgattc cggtccgtcc gccgatctcg acctcgcgga cgatacggcc
     2221 gtcgaacgtg gaaccgatca ccgaggcatg ggtcatcgag tggccgacgt gcatccgacc
     2281 ccgggcgtgg agcgtggcga tccgcgccga caggccggtg cccgtcgccg accggtcgag
     2341 cctgccgggc gcgacgatga ccgcgttctt cgagatccgg ccgttgcccc gccacggctc
     2401 ggcgagctcg atgacgctca cctccctgat ctcggggttc tccggatgga ccggatggag
     2461 ctgttcgcgg gcagccttgt ggatgcgcgt gcccatgcgc gagaggtccg ctgcctcgga
     2521 cggttccagg gcgaatccga gcgcagcggc gtcggcgatc gcgtaccaca tgcccccgaa
     2581 cgcgacgtcg accttgatcg tgcccaggcc ctcgacctcg agcggggcgt cgaggcggtc
     2641 cgcgaagcag ggcacgttct cgagctcgac ccattcgacc ttgccggcgc ggcaaccggc
     2701 ggtgatgtcg atcgggccgc ccggcgcctc gagccgaaag gtcgtcgtcg gttcgtgcat
     2761 ctcgaccatg cccgtctcga gcaggaccgt ggcgacgcag atcgtgttcg agccggacat
     2821 cgccggatac tcggtcggct ccatgatcac gtagccgaag gcgcaatcct cgcgcagggc
     2881 cggcagaaca atgttcgaat ggaccgccac gctgccgcgg ggctcgtgga ggagcagctt
     2941 ccggatcgag tcatccgcgg cgatggcctg catctgctcg aagaccgtcg cgcccggcgg
     3001 cggctcgacg cccgcgacga cgacgttccc gatctcgccg cccgcgtggc acccgacgac
     3061 cgtgaggacg gggcgggcgg gcgtccagcc gactgagccg agcggatccg ggaaagtggc
     3121 catcttcgag ggtgatgatg ccggtccgag cgcgatgccg cccgacaact gcgccgccag
     3181 cccttgacat cctgtcacac cctgatatac gtatacgcct acgtatagct acggtggttg
     3241 cgaaggggct aggatgtgga tgtgccaaac aagacgatct acgtctccga cggagacctc
     3301 gggatctacc agcgcgccca ggagatcgcg ggcggcaacc tctcctcggc catcgccaac
     3361 gcgctccggc gc
//
LOCUS       DATBSW010000168         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1038387, whole genome shotgun sequence.
ACCESSION   DATBSW010000168 DATBSW010000000
VERSION     DATBSW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038387"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VIF63_04685"
     CDS             complement(<1..827)
                     /locus_tag="VIF63_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8238711.1"
                     /translation="MPAITVPDITVLPRVPEPDPAVARQRPVQSITNAPSGVEGEGFP
                     VRRAFHGVDMSELDPFVHLDQMGEVEYAPGEPKGTPWHPHRGFETVTYMIDGVFEHND
                     SNGGGGTITNGDTQWMTAGAGILHIEKPPAWLVASGGLFHGFQLWVNLPAAQKWSPPR
                     YQDIRANQVALATSPDGGALVRVIAGDIAGQAGPGSTYTPMTLVHATLAPGARLSLPW
                     RSDYNALVYVMQGHGSVGAAPSFRAGSSLAGGAASGAEPQPIRTGQLAVFGPGDALTV
                     "
     gene            1080..2498
                     /locus_tag="VIF63_04690"
     CDS             1080..2498
                     /locus_tag="VIF63_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8238712.1"
                     /translation="MTLTAHALEGPIRQLKQTIAGRVIEPGSEDYDSARKVMMGNIDR
                     HPAAIVRVANVDDVKAVVALAGATGVELAIRSGGHSAKGDSTTEGGIVLDLRDLRKID
                     IDPVARTAWAETGLTANEVTKAAAEHGLAIGFGDTGSVGIGGITTGGGIGYLVRKYGL
                     TIDNLLAADVVTADGELRRADQITNPDLFWAIRGGGGNFGVVTRFQYRLVDLPEIVGG
                     MLFLPATAETVERFVTLAEEAPEEMSTIANVMPCPPMPMVDKAWHGKLVIFGLMCFAG
                     GAEAGAKAFQPFRDLAKLGGLDAPIADLVKPQTYPDMYPPDGPGDEDYHPLAVSKNLL
                     INRVDRPTAETVMRYLEALDAPMRVAQIRVMGGAMARIPWDATAFAHRSFKVLVNVAA
                     FYEDEADRVRKHAWMDAFAAAIQAASGATSDCAYVNFVGDEGEARIHDIYPDATLARL
                     TEIKRRYDPTNVFRLNQNIPPA"
     gene            2641..2910
                     /locus_tag="VIF63_04695"
     CDS             2641..2910
                     /locus_tag="VIF63_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238713.1"
                     /translation="MPGLDLIGWILIGLLAGSISGWFVRGTRSVQGCLPTIVVGIIGG
                     VIGGYLARGVGNGPVTGFLGALVFATIGSMAIRIVLRAIETGSKR"
     gene            3007..4008
                     /locus_tag="VIF63_04700"
     CDS             3007..4008
                     /locus_tag="VIF63_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEY8238714.1"
                     /translation="MPSYSRTSATVDAPPAPVDAPAAPVDAPPEERDLLVELEPVVAK
                     LYDRHASMAQEWFPHDFIPYRLGRDFDKEPWTPDQPRLTGVAQTSFEIGLLTEDNLPS
                     YHRLIHGMFGHGDGAWLAWVGRWTAEEGRHAIVLRDYLTVTRNIDPVYLERGRMTQLM
                     QGWEMNSTDTLHGLAYVTFQELATRIAHRNTGRYSDDPVADKIMIRIATDENLHMVFY
                     RDVLSAALKLQPSAAVRAIVDEVLNFKMPGAGIPGFIRKAAQIAKAGIYDLKVHRDEV
                     LMPILRHWKIFELSDLDATAEDARRRLSEYLARLDQQIARFEERLALSTVPRIATLK"
     gene            4705..>5358
                     /locus_tag="VIF63_04705"
     CDS             4705..>5358
                     /locus_tag="VIF63_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8238715.1"
                     /translation="MTRGPIPRFDLYEELEVSRFASAEVIEAAYRTLVKRHHPDLGEA
                     ADPDRIKRLNVAHGWLTSPSLRARYDETTDSLPRPGTTARKGVDASEATQDLAPGGFG
                     PNSAEVRQFLADLRPLDVARAQEMRSGKATLGTTQYAEARGAALAASRTGGRHDQWLF
                     ARDAAMVIVKGKLGDWPFGAEVAAIVADVAGAMVVRDLIPLADFQTVLTPWTWRPDAA
                     "
BASE COUNT          863 a         1903 c         1797 g          795 t
ORIGIN      
        1 acggtgagcg cgtcgcccgg gccgaagacg gcgagctggc cggtccggat cggctggggc
       61 tcggcgcccg aggcggcgcc gccggccagc gacgagccgg cacggaacga cggcgccgcg
      121 ccgaccgacc cgtggccctg catcacgtac accagcgcgt tgtagtcgga gcgccacggg
      181 agcgagaggc gggcgcccgg cgccagggtc gcatggacca gggtcatcgg tgtgtaggtc
      241 gagccgggac cagcctggcc cgcgatgtcg ccggcgatca cccggacgag cgctccccca
      301 tcgggcgacg tagcgagcgc gacctggttc gcgcggatgt cctggtagcg cggcggcgac
      361 cacttctgcg ccgccggcag gttcacccag agctggaagc cgtggaatag cccgccgctc
      421 gcgacgagcc aggccggcgg cttctcgatg tgcaggatcc cggcgcccgc ggtcatccac
      481 tgcgtgtcgc cgttggtgat cgtcccgcca ccgccgttcg agtcgttgtg ctcgaacacg
      541 ccgtcgatca tgtacgtcac cgtctcgaag ccgcgatgcg ggtgccacgg cgtgcccttg
      601 ggctcgcccg gtgcgtactc gacctcgccc atctggtcga ggtggacgaa cgggtcgagc
      661 tccgacatgt cgacaccgtg gaacgcgcgt cggaccggga acccctcgcc ttcgacgcca
      721 gacggggcat tggtaatcga ctgaaccggt cgttggcggg cgacggcggg atcgggctcg
      781 ggaacgcggg gcaggacggt gatgtcgggg acggtgattg cgggcatgcg caaatgattg
      841 atcagtcaac tacttctcgt caaatccctg tttgcaccaa atgtctccgt agggcacaca
      901 agcgtccacg tccggcctgg tcgcggaatc ttgcggcccg tgctgaacgc gagcgaacga
      961 tccggcgagc gtgcacggcc gcgcctcaat tggcaggatc cggcgatcct tggcaggatt
     1021 cgcacctcgc aactgcccgc gatccgcgtg atccctgcaa tcccacccgg aggtgcgtct
     1081 tgacgctcac cgcacacgcg ctcgaaggcc cgatccgcca gctcaagcaa accatcgccg
     1141 gtcgcgtgat cgaacctggc agcgaggact acgactcggc gcgcaaggtg atgatgggca
     1201 acatcgaccg ccatcccgcg gcgatcgtcc gcgtcgcgaa cgtcgatgac gtcaaggccg
     1261 tggtcgcgct cgccggcgcg accggcgtcg agctcgcgat ccgcagtggc ggccacagtg
     1321 ccaagggcga tagcacgacc gagggcggga tcgtcctcga ccttcgtgac ctccggaaga
     1381 tcgatatcga ccccgtcgcc aggaccgcct gggccgagac cggcctcacg gcgaatgagg
     1441 tcaccaaggc agcggccgag catgggctcg cgatcggctt cggggacacc ggcagcgtcg
     1501 ggatcggcgg catcacgacc ggtggcggca tcgggtatct cgtgcgcaag tacggcctca
     1561 cgatcgacaa cctgctcgcc gcagacgtcg tgaccgcgga cggtgagctc cgccgcgcag
     1621 accagatcac gaacccggac ctgttctggg cgatccgtgg tggtggcggc aacttcgggg
     1681 tcgtgacgcg gttccagtac cggctcgtcg acctgcccga gatcgtcggc gggatgctct
     1741 tcctgcccgc gacggcggag accgtcgagc gcttcgtcac cctcgccgag gaagcgcccg
     1801 aggagatgtc gaccatcgcg aacgtgatgc cctgcccgcc gatgccgatg gtcgacaagg
     1861 cgtggcacgg caagctggtg atcttcggcc tgatgtgctt cgcgggtggc gccgaggccg
     1921 gcgcgaaagc cttccagccg ttccgcgacc tggcgaagct cggtggcctc gatgccccga
     1981 tcgccgacct cgtcaagccg cagacgtacc cggacatgta cccgcccgac ggccccggcg
     2041 acgaggacta ccacccgctc gcggtcagca agaacctgct gatcaaccgc gtcgaccgcc
     2101 cgacggccga gaccgtgatg cgatacctcg aggcactgga cgccccgatg cgcgtcgcgc
     2161 agatccgggt catgggcggc gcgatggccc ggatcccatg ggacgccacc gcgttcgcgc
     2221 atcgcagctt caaggtcctg gtcaacgtgg ccgcgttcta cgaggatgag gcggaccggg
     2281 tccggaagca cgcctggatg gatgcgttcg ctgcggcgat ccaggcggcc tcaggcgcga
     2341 cgagtgactg cgcctacgtg aacttcgtcg gggacgaggg cgaggcccgg atccacgaca
     2401 tctatcccga tgcgaccttg gcccggctga ccgagatcaa gcgccgctac gacccgacga
     2461 acgtgttccg cctgaaccag aacatcccgc cggcctagcg ccgggaacat ccctcgtcac
     2521 cgccgtgatg cctatgggtg catcacgctc cactgattac cccataggca tcgccggtcg
     2581 catgagtaac gctcacgcgg ggcgcgcgcg actagcccgt gccgtgcgac gatgcacgcc
     2641 atgccaggtc tcgacctcat cggctggatc ctcatcggcc tgctggccgg ctcgatctcg
     2701 ggctggttcg tgcgcggcac gcgcagtgtc cagggctgcc tgccgacgat cgtggtcggg
     2761 atcatcggcg gggtcatcgg cggctacctg gcgcgcggcg tcggcaacgg gccggtcacc
     2821 gggttcctcg gcgcgctcgt gttcgcgacg atcgggtcga tggcgattcg catcgtcctc
     2881 cgcgcgatcg aaaccgggtc caaacgctga cctgactcgc cctaccgtgc gagattcgaa
     2941 cggacgatcg atgcaggtcc acaccgaacc ggcgcacggt cggcccccga gccctacgct
     3001 catcggatgc cgagttactc ccgaacgagc gcgacggtcg acgcgccgcc tgcccccgtc
     3061 gacgcgcccg ccgctccggt cgatgcgccg cccgaggagc gcgacctcct cgtcgagctc
     3121 gagccggtcg tcgcaaagct gtacgaccgc cacgcgagca tggcccagga gtggttcccc
     3181 cacgacttca tcccctaccg cctcgggcgc gacttcgaca aggagccctg gacgcccgac
     3241 cagccgcggc tgaccggcgt cgcccagaca tcgttcgaga tcgggctcct gaccgaagac
     3301 aacctgccga gctaccaccg cctgatccac ggcatgttcg ggcacggtga tggcgcatgg
     3361 ctggcctggg tcggacggtg gaccgcggag gagggccggc acgccatcgt gctgcgcgac
     3421 tacctgacgg tcacccgcaa catcgacccg gtctacctcg agcgcgggcg catgacccag
     3481 ctcatgcagg gctgggagat gaactcgacg gacacgctcc acggtctcgc gtacgtgacc
     3541 ttccaggagc tggcgacgcg gatcgcgcat cgcaacaccg ggcgctactc cgacgacccg
     3601 gtcgcggaca agatcatgat ccgcatcgcg accgacgaga acctgcacat ggtcttctac
     3661 cgcgacgtgc tctcggccgc gctcaagctc cagccgtcgg ccgccgtgcg ggccatcgtc
     3721 gacgaggtcc tcaacttcaa gatgcccggc gccggcatcc ccggcttcat ccgcaaggcc
     3781 gcccagatcg cgaaggccgg catctacgac ctcaaggtcc accgcgacga ggtcctgatg
     3841 ccgatcctcc ggcactggaa gatcttcgag ctgtccgacc tcgacgcgac cgccgaggac
     3901 gcccgccgtc gcctgtccga atacctggcc cggctcgacc agcagatcgc ccgcttcgag
     3961 gagcgcctcg ccctctcgac cgtcccgagg atcgccaccc tcaagtaggc cgcgaaggcg
     4021 ggtggctcca ggcgcccggc tgtcgcgccc ttcgtatgcg cgccgttcgt atgcgctccg
     4081 ttcgtatgcg cggattcgac gcagataatc ccgtcgcgct cggcgatgcc cgtcgccagc
     4141 ataggagtgg gtacgaccgc cacggcgtgc gccggccgcc gtgatgagcg acgggtgatc
     4201 cagaggtgac ccgcccgtaa gcgccgaagg ccatcctcgg cggatgaatc ggtgggcgtg
     4261 ggtcggtgat gacaacgcgg cgcaggatct cgggctgagg atcctcgccc atgccatccg
     4321 tggccggcgc gtcgcgtccg gcatcagtca gatgcagctc gcttatcgag tcggcttgaa
     4381 tcagtcgacg atctcgcggc tcgagacagg tcggttgcgc gcgatgcgca tggtcgtgct
     4441 ggcgcgcgtg atcggagcac tcaacctgcc gcctggtgac ctatctccga acgagccgcc
     4501 accaccaagg cgtcggatgc ccggccagcc atcgacgtag cgcgcgagcg gcgccgcagc
     4561 gccattgcgt atgtatgccg gcaatgggca ttgccgcgcg cgatagaact gccaaggccg
     4621 attccgcgca tatgaggcat cgcgggtccg cgcatttcag gcaaagcagg tctcggggga
     4681 ggcgggcccg tacacttgcc cccggtgacc cgcggaccaa tccctcgctt cgacctctac
     4741 gaggagctcg aggtctcccg cttcgcgagc gcggaggtca tcgaggcggc ctaccgaacg
     4801 ctggtgaagc ggcatcaccc cgacctcggc gaagctgccg accccgaccg catcaagcgg
     4861 ctcaacgtcg cccacggctg gctcacctcg ccgtcgctcc gcgcccgcta cgacgagacg
     4921 accgacagcc tgccgcggcc cggcaccacg gcgaggaagg gcgtcgacgc gagcgaggcg
     4981 acacaggacc tcgcacccgg cggcttcggc ccgaacagcg ccgaggtccg tcagttcctc
     5041 gccgacctcc gcccgctcga cgtggcccgg gcccaggaga tgcgctctgg gaaggccacg
     5101 ctcggcacga cccagtacgc ggaggctcgc ggggccgcgc tcgcggcgag ccgcaccggc
     5161 ggccgccacg accagtggct cttcgcccgc gatgccgcga tggtcatcgt caagggtaag
     5221 ctcggcgact ggccgttcgg cgctgaggta gcggcaatcg tggccgatgt cgcgggtgcg
     5281 atggtcgttc gcgacctgat cccgctggcc gatttccaga cggtcctcac gccctggacc
     5341 tggcgccccg acgcggca
//
LOCUS       DATBSW010000169        12346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_50879, whole genome shotgun sequence.
ACCESSION   DATBSW010000169 DATBSW010000000
VERSION     DATBSW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50879"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VIF63_04710"
     CDS             complement(<1..842)
                     /locus_tag="VIF63_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238716.1"
                     /translation="MSSTMLKEPKMTQDRAIDPVRLPAPVAAPSPAPVLGPVAEPAVP
                     ALLVDRVTKKFNVSRKKEPVTAIADVSLRLERGGTIGILGANGSGKSTLIRLVSGLLT
                     IDEGRVEVFGLDLVREEMAVKRLINRVSVDAAFFKKLSPMENLLFAARLYGIDSAFAR
                     TETVRITERLGIAAKRLSRPVEQLSRGMQQKIAIARALLTSPALLLLDEPTTGLDPRS
                     KLDVQAFIEELRDTHDATIVLTTHDLAEAERLCGRITILNDARVVAEATPDGLKAMVA
                     AATGR"
     gene            1222..2001
                     /locus_tag="VIF63_04715"
     CDS             1222..2001
                     /locus_tag="VIF63_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238717.1"
                     /translation="MSGDAPPSHAGPADPSIVAVLAQGVMDAELAALVWLLVEARVPL
                     VVAAPAGRAGAGAQLLAGIIGSIHPDEQVDGLRAPLTPAGASSLVRGRRAGGVVEADS
                     LEDVRRKLGSGPLPLSDDQLTFIGCVLVLADGSEAKRGRLRVTAAHYVRPLARDAHGH
                     SQRLDPAVLAAWDPRLERYEHFAWGVLPEIAARLGRRSGDLEADLHHRRDDLAGLVKA
                     GLTEIGEVRRLIAGYQTRWGDGHDAKRHDEPHAEGPGHQPH"
     gene            2085..2576
                     /locus_tag="VIF63_04720"
     CDS             2085..2576
                     /locus_tag="VIF63_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY8238718.1"
                     /translation="MSLFPWFLFAHVLGAIIAFGPTFSSPIIGRMGGAERAHSNFAMR
                     VSHELARVQILPLAIVQGLTGVGLIVTGSIDLFKDHNWLLVGIALYLFAIGYAIFVQT
                     PLANRIIALTTPPAGAPAPAGPPAGGPPPELMGLITRIKRGGMLLIVLVVSIVFLMVT
                     KPF"
     gene            2633..4051
                     /locus_tag="VIF63_04725"
     CDS             2633..4051
                     /locus_tag="VIF63_04725"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY8238719.1"
                     /translation="MPNVERSVADLLSRLSGIVHVHGDEIEIVDVGRLRADGIRDLAW
                     TAAFGEDPATVEVAQWIVWEAAQELGARSASIHELYMARARGEVSGFTVPAINIRAQT
                     FDMARIAYETAETFDVAAVILELARSEQTYTFQRPIDYSTAVLAGAIAAHWQGPVFLQ
                     GDHYQFNAKKYATDPESMTEEIRRACRLAVDAGYRNIDIDASTLVDLSLPTDEEQQRA
                     NYERTAELVALTRTLEVDGVTISMGGEIGEVGKENSNEAELRAYLDGLRRELAKAGAG
                     GENGVGISKVSVQTGTSHGGVVLPDGTVGTAAVDFSIHEKLGVVARREYGLAGTVQHG
                     ASTLPDELFHRFREVETAEIHLATGFQNALYEHPAFPREMHEQIESWCFANALDERKS
                     DQTDQQFVYTTRKKALGPFKRQLWELETKDEILADQAKKIGFLCRELGVTDSRAMVEK
                     HIRPVAWHRPMPAALREVAAAH"
     gene            4469..6052
                     /locus_tag="VIF63_04730"
     CDS             4469..6052
                     /locus_tag="VIF63_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8238720.1"
                     /translation="MSGPGSAADVAAKDDAHLRSLGIKPELRRSFGFLSNFAVAFSYI
                     SVSTGTYTLIALGLGVGGPAFFWSWPLVVIGQTFVALNFAELSSHFPVAGSIYQWSKR
                     LSNKTLGWFTGWFYFWAGVLTTTAVAATVPLVLSAIFGFDLADPSPIGAVNNLVFWGL
                     ISLISTTFINAFGIRLLQIINNLGVAAEILGMLVFAVILLVVGRHQDLSVLTQTAGTE
                     TAETGGYLPIFAIAMFMSLFVVYGFDTAGTFGEETLDASRQAPRGILSAIWISGLIGF
                     VFLLAIVLAIPDVAASLVDPAPIATSIKAGLGETFGNVYLYVILAAVYVCTMAIQGAT
                     TRLMFSMGRDRRLPLGGMWAHVNPTFKTPANAAIAVGVLAAIPFVVTDSPVLLAAGAT
                     GLIYLSYFLCNLGVFNARRKGWPHKGAWFSLGSWGTIINIVALVWGGVMILNFGLWSD
                     SGLFGNFGSDLRDLTNPSLTLITSGGNTIGFLPDIPFFEASILLILVVGLVYYYATGQ
                     GRREDKVEADLATGEAMIA"
     gene            6220..7191
                     /locus_tag="VIF63_04735"
     CDS             6220..7191
                     /locus_tag="VIF63_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8238721.1"
                     /translation="MTTQRGALEDELRGELRDELARALHRLPELGMGGLTFTALSGGI
                     TNRNFLVSGAPGGARFVVRLAGNDTHLLGISREVEHAATVTAAGLGVGPEVIAYLRPE
                     GYLVTRFIEGGPIAEAEMRQPARLRQVGATLRRIHDGPAIPGLFVPFRIVESYRALAI
                     SRNVPIPPEFDLAQSIARRIELACLMAPIEMRPCHNDLLNANFLDDGSRIRIVDWEYA
                     GMGDPYFDLGNFSINHELDADQDAILLAAYLGLDAGAVAPRPLAARLTLMRVLSDFRE
                     AMWGVLQQGCSTLDVDFVAYAAEHFDRLLTVAGRPAFERALTDAAGG"
     gene            7237..9684
                     /locus_tag="VIF63_04740"
     CDS             7237..9684
                     /locus_tag="VIF63_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8238722.1"
                     /translation="MADQARVVVIGGGITGVSVAYHLAEAGWTDVLLVEKAGLTAGST
                     SQAAGLVTAFNPSSTMLAWRRHSIELYGRLGVFDAVGSVRLASSPEQLKELERTASRA
                     RGVGLDVGVISAEEARRLLPAISPDNLYGAVYLPGDGYLDPHTATFAVADAARKLGVR
                     IRQNTRVTGFDTGLGPRREIRRVLLENGEAIDTELVVNAAGMWAPQVAEMVGAFIPST
                     PVDHQHVALRAVPGHELPNDMPAFRDPDNLVYGKAEHGGMLFGGYENAPHARWETGVP
                     WDHAATSLPPDWDRFQPLMEGAIRRFPFLADAEAVRLVCHPDAMTPDANPLIGPMPGV
                     RGFWVAAGLSLNGFGGGGGIGKALAGWITSGDPGVDIAPYRAWRFADTYRDPEWTSGL
                     ARETYADYYRLRFPYDADVAGRPRRLSALHQRLGQAGAVFGTKAGWERADLHQPGQRW
                     TRNGRLPRERGWARPDWFERVGEEHRAVRERVGIIDLSSFGKIAVAGPGALALLQRVA
                     ANDIDRPIGSTIYTQFLDERGGIVADVTVTRDYIDRFQVVTGSGYLAADLGWLVARAA
                     DEPAIDGVTIDDRTDAWSVIGLWGPRAREVLAGITKDAVDDASLPIRQARSIRVGGFG
                     VTATRLSYAGELGWELLVEPANAVEVWDGLLASGRPQGIEPVGYRALESLRLEKGYRY
                     FGAELTPRETPFEAGLERFVRFGKGDFVGRDALLARRAAEPDGPARRLRTVLIGGSDW
                     APIYGGEAVRVDGEVVGRLRSAAFGYTVGRTVGTTYLPARIAEGAAIEVDVFADRIAG
                     EVAADVLVDAAGDRMKA"
     gene            complement(9697..10518)
                     /locus_tag="VIF63_04745"
     CDS             complement(9697..10518)
                     /locus_tag="VIF63_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8238723.1"
                     /translation="MADLAGIPANLRPGAFAGAAEAYARYRPPYPPHVLADLLDRAAV
                     GREALLDLATGPGRIALDLAPRFARVVAVDLEPDMIDVARREAARRDIKTVTWHVGRV
                     ETFEAQTESFDLVTIGEAFHRLEAAVVARNVFRWLRPGGCLATLGTDNRFSGDEPWEV
                     TYRVVRDRWIARSFPEGFGLVLPGGALDQADREAAMRSAGFVDIQGHDFEQTVQLSFD
                     ELVGYLASTSGCSRKAIGPDFDVWIDELRSTLGADDSTRFTEHVRWGYTLARKPG"
     gene            complement(10533..11456)
                     /locus_tag="VIF63_04750"
     CDS             complement(10533..11456)
                     /locus_tag="VIF63_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEY8238724.1"
                     /translation="MDPARPGIEPVLARLWPGRDVEVSPIAAGLTNQNFRVEVDGRPC
                     FVRLPGASTDLLAVDRANELHNTRAAAEAGVGPKVVEVDPETGAFALEWIDGRTMSNT
                     AFAEPGAPARIAEALRRLHAGPRFRDAFDMFRLTEFYLRVVDERSIRIPDGYREHLDQ
                     LPRIEAALAAQPLATVPCHNDLLAENYLDDGSKLWIVDYEYSGNNDPTFELGNTAQEL
                     GFDPARQEELCAAYFGPERFALEGRALVARMRLQMIMSDVGWTLWAAIQAAISTIDYD
                     FWGWAEERWGRASTALDRPDFDDLMGAASEG"
     gene            complement(11456..>12346)
                     /locus_tag="VIF63_04755"
     CDS             complement(11456..>12346)
                     /locus_tag="VIF63_04755"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY8238725.1"
                     /translation="FALLAFVLVLRDRPLLAAIAITAAILTKPQALALALPFAAWYLA
                     TYDRRTVIRAGMTAVVVAIALWLPFLQWDGPSKYLGWLAYYHDERFDVISMRAWNVWW
                     IVQRSILHVSWVPDEDSLVGPLTYRMAGYLITVALAALVAYRVWRRPTPVVLALSLAA
                     STLIAFTFLTTMHDRYSYAAIVFMALLPDRRRMVAFSAVLGFLVLWNVTASASVARMF
                     HVPMELSGNSGLTGSLGFIVLTLVSVALVWAERRDAQSALGDTTARDAPAEVDTDPES
                     AAPAAAAPDAAAPNPAAAAT"
BASE COUNT         1844 a         4239 c         4325 g         1938 t
ORIGIN      
        1 cgacccgtgg cggcggccac catggccttg agtccgtccg gtgtcgcttc ggcgaccacc
       61 cgggcgtcgt tgaggatcgt gatccggccg cacaggcgtt ctgcctcggc caggtcgtgc
      121 gtggtcagca cgatggtggc gtcatgcgtg tcgcgcagct cctcgatgaa ggcctgcacg
      181 tccagcttcg accgcgggtc gaggccggtc gtcggctcgt cgaggagcag gagcgccggg
      241 ctggtcagca gggctcgcgc gatcgcgatc ttctgctgca tgccccggct cagctgctcc
      301 accggccggc tcaggcgctt cgcggcgatg ccgagtcgct ccgtgatccg gacggtttcg
      361 gttcgagcaa acgccgagtc gatcccgtag agcctcgctg cgaacagcag gttctccatc
      421 gggctgagct tcttgaagaa ggcggcgtcg acgctcaccc ggttgatgag ccgcttgacc
      481 gccatctcct cgcggacgag atcgaggccg aacacctcga cccggccttc gtcgatcgtc
      541 agcaggcccg agacgagcct gatgagcgtc gacttgcccg agccgttggc gcccaggatc
      601 ccgatcgtgc cgccgcgctc gaggcgcagc gagacgtccg cgatcgcggt caccggctcc
      661 ttcttccggg acacgttgaa cttcttcgtg acccggtcga cgagcagggc gggcaccgcg
      721 ggctccgcca cggggccgag cacgggagcc gggctcggcg ccgccacggg ggcgggcagc
      781 cgcacgggat cgattgctcg gtcctgcgtc atcttcggtt ccttgagcat ggtgctcgac
      841 atggttggag tccttctggg ctggtgaaca tgaaaaaaag ccgcctcgcc ttcccgtgca
      901 gtgcgggtgg ctcgcggctc gtcagtggtc cccgtggtgc agcctgggga caaatgaaaa
      961 accgtgggcc cgatctggcc cacggtcgtg gtagctggtg gaagcggcac tgccgcctgt
     1021 ccgttacctc gaggtgggcg caaatctccc gccaccgggc gcgctgatag gcgcgaccag
     1081 tcgccgggcg agcggggtgc tcttcatcac atctacctcc gataaccgac atcgacgccg
     1141 tggcgccgag gccggagtct atggtcgggt cgcagctgct gtcaatttcg tgcggggaac
     1201 gggtcggcta ggctcgacgc catgtccgga gacgcgccac cgagccacgc cggcccagcc
     1261 gatccgtcga tcgtcgcggt cctggcgcag ggcgtgatgg acgccgagct cgccgccctc
     1321 gtctggctcc tcgtcgaggc ccgggtgccg ctcgtcgtgg ccgcgcccgc gggccgcgcg
     1381 ggtgccggcg cccagctgct ggccggcatc atcgggtcga tccatcccga tgagcaggtc
     1441 gacggcctcc gcgctccgct cactccggcg ggtgcgagca gcctcgtccg aggccggcgt
     1501 gccggtggcg tcgtcgaagc cgattcgctg gaggacgtcc gccggaagct cggctccggg
     1561 ccgctgccgc tgtcggacga ccaactcacg ttcatcggct gcgtgctcgt gctcgcggac
     1621 ggcagcgagg cgaagcgcgg tcgactccgg gtgaccgcgg cgcactacgt ccgaccgctg
     1681 gcgcgcgacg cgcacgggca ctcgcagcgg ctcgacccgg cggtgctggc cgcctgggac
     1741 ccccgcctcg agcgctacga gcacttcgcc tggggcgtcc tgcccgagat cgcggcgcgc
     1801 ctcggccggc gtagcggcga cctcgaggcc gacctccacc accgccgcga cgaccttgcg
     1861 ggcctggtca aggccggcct caccgagatc ggcgaggtgc ggcgcctgat tgccggttac
     1921 cagacgcgct ggggcgacgg ccacgatgca aagcgccacg acgagccgca cgccgaaggg
     1981 cccggtcacc agccgcactg acggcggcct gcaagccccc gtcgttccgg tgcatccttc
     2041 cgcacgacat ccggggcatc gagccgcagg tcgaggaggt cagggtgagc ctgtttccgt
     2101 ggtttctgtt cgcccacgta ctgggcgcga tcatcgcctt cgggcccacg ttcagcagcc
     2161 cgatcatcgg gcggatgggc ggtgcggagc gggcacacag caacttcgcg atgcgggtca
     2221 gtcacgagct agcccgcgtg cagatcctcc cgctggcgat cgtccagggc ttgaccggcg
     2281 tcggcctgat cgtcaccggc agcatcgacc tgttcaagga ccacaactgg ttgctcgtcg
     2341 ggatcgcgct gtacctgttc gcgatcggct acgcgatctt cgtccagacc ccgctcgcca
     2401 accggatcat cgccttgacc accccgcctg ccggcgcccc ggccccggct ggcccgccgg
     2461 ccggcggtcc gcctccggag ctcatgggcc tgatcacccg gatcaagcgc ggcggcatgc
     2521 tgctgatcgt gctcgtcgtc tcgatcgtct tcctgatggt gaccaagccg ttctgaccgg
     2581 gcgctgatcg tcgacgaggc cgtctcgggc ggcccggcga taccatctgt tcgtgcccaa
     2641 cgtcgaacgg tccgtcgccg acctgctctc gcgcctgtcc gggatcgtgc acgtccacgg
     2701 cgacgagatc gagatcgtcg acgtggggcg attgcgcgcc gatggcatcc gcgacctcgc
     2761 ctggacggcc gcgttcggcg aggatcccgc gaccgtcgaa gtcgcccagt ggatcgtctg
     2821 ggaggcggcc caggagctcg gcgcgcgctc ggcgagcatc cacgagctgt acatggcccg
     2881 agctcggggc gaggtcagcg gcttcaccgt cccggcgatc aacatccgcg cccagacgtt
     2941 cgacatggcc cggatcgcgt acgagaccgc cgagaccttc gacgtggcgg cagtcatcct
     3001 cgagctcgcc cggagcgagc agacgtacac gttccagcgc ccgatcgact actcgaccgc
     3061 ggtcctcgcc ggcgccatcg cggcgcactg gcagggcccg gtcttcctcc agggcgacca
     3121 ctaccagttc aacgccaaga agtacgcgac cgacccggag tcgatgaccg aggagatccg
     3181 gcgtgcctgc cgcctggcgg tcgatgccgg ctatcgcaac atcgacatcg acgcgtcgac
     3241 gctcgtcgac ctgtcgctgc cgaccgatga ggagcagcag cgcgccaact acgagcgcac
     3301 cgccgagctc gtggcgctga cgcgcacgct cgaagtcgat ggcgtgacga tcagcatggg
     3361 cggcgagatc ggcgaggtcg gcaaggagaa ctcgaacgag gccgagctgc gcgcctacct
     3421 cgacgggctt cgtcgcgagc tcgcgaaggc aggtgcgggc ggcgaaaacg gcgtgggcat
     3481 ctcgaaggtg agcgtccaga ccgggacgtc acacggcggc gtcgtgctgc cggacggcac
     3541 ggtcggcacc gcggccgtcg acttctcgat ccacgagaag ctcggggtcg tcgcgcggcg
     3601 cgagtacgga ctcgccggga ccgtccagca cggcgcgtcc acgctgccgg acgagctgtt
     3661 ccaccgattc cgcgaggtcg agaccgccga gatccacctc gcgaccgggt tccagaacgc
     3721 gctctacgag caccccgcgt ttccgcgcga gatgcacgag cagatcgagt cgtggtgctt
     3781 cgcgaacgca ctcgacgagc gcaagtccga ccagaccgat cagcagttcg tgtacacgac
     3841 gcgcaagaag gcgctcggac cgttcaagcg ccagctgtgg gagctcgaga cgaaggacga
     3901 gatcctcgcc gaccaggcga agaagatcgg cttcctttgt cgcgagctcg gcgtcaccga
     3961 cagccgggcg atggtcgaga agcacatccg cccggtggcg tggcaccgcc cgatgccggc
     4021 cgcgctgcgc gaggtcgccg cggcgcacta gatcgcggct gccctcggca tcccccgcga
     4081 gacccgctgc tcgcaaatcg cgtcgtcctt ccattggatg gacgatcggg cgggcgagtg
     4141 tgacccaaac tacgaattcg gcctcttcgc gctccggctc atccatcaga tgttcaagcc
     4201 ggcgggggaa cggccctcgc cgcaggctca cccgccggaa cgtgcatcga atgcacgaat
     4261 cacggccgac gcgcgccagg ttcccgcgaa acgcagacat ggatcgttgc ggaatcgcct
     4321 tgattcgtgt ttctggcgct cttatgctcg ccccgcgttc gggttgaggg acccgacgag
     4381 tgccccgcga tcctcggagt tccggagagg cgtaactcgc ggcccgccgc ccaagcctgc
     4441 ggtgccgcgt gggtagaggg aggaagccgt gtcgggtcca ggcagcgctg cggacgtcgc
     4501 cgcaaaggac gacgcccacc tgcgatcgct gggcatcaag cccgagctca ggcgatcgtt
     4561 cgggttcctg tcgaacttcg ccgtcgcgtt cagctatatc agcgtctcga cgggaacata
     4621 cacgctcatc gcgctcgggc tcggcgtcgg cggtccggca ttcttctggt cctggccgct
     4681 tgtcgtgatc ggccagacct tcgtggccct gaacttcgcc gagctgtcga gccacttccc
     4741 cgtcgccggg tcgatctacc agtggtcgaa gcgtctctcc aacaagacgc tcggctggtt
     4801 taccggatgg ttctatttct gggccggcgt cctgacaacg accgcggtcg cggccacagt
     4861 gccgctggtc ctctcggcga tcttcgggtt cgacctcgcc gatccgagcc cgatcggcgc
     4921 cgtgaacaat ctggttttct ggggcttgat cagcctgatc tcgacgacgt tcatcaacgc
     4981 cttcggcatt cgactcctgc agatcatcaa caaccttggt gtggccgccg agatcctcgg
     5041 gatgctcgtg tttgccgtga tcctgctcgt cgtcggccga caccaggact tgtccgtcct
     5101 gacccagacg gcgggcacgg aaacggcgga gacaggcggc tacttgccca tcttcgcgat
     5161 cgcgatgttc atgtccctct tcgtcgtgta cggcttcgac acggccggca cgttcggcga
     5221 ggagacgctc gatgccagcc gccaggcgcc ccgcggcatc ctgtcggcga tctggatctc
     5281 gggcctgatc gggttcgttt tcctcctcgc gatcgtcctc gcgatccctg atgtcgccgc
     5341 atcattggtc gacccggccc cgatcgccac atcgatcaag gccggtctcg gcgagacctt
     5401 cggcaatgtc tacctgtacg tgatcctggc ggccgtctat gtctgcacga tggcaatcca
     5461 gggcgccacg acccggctca tgttctcgat gggtcgcgac cgtcgcctgc ctctcggtgg
     5521 catgtgggcg catgtgaatc cgaccttcaa gacgccggcg aacgccgcca tcgcggtcgg
     5581 agtcctcgct gccattccat tcgtcgtcac tgacagcccg gtcctcctcg cggcgggcgc
     5641 gacgggcctc atctacctca gctatttcct ttgcaacctc ggcgtcttca acgcccgccg
     5701 caagggctgg ccgcacaagg gcgcttggtt ctcgctgggc agctggggca cgatcatcaa
     5761 catcgtcgcc ctcgtctggg gcggcgtgat gatcctcaac ttcgggctct ggagcgacag
     5821 cggcctgttc gggaacttcg ggagcgatct ccgggatctc accaatccgt cgctgacatt
     5881 gatcacctcg ggcgggaaca ccatcggttt cctgcccgat atcccgttct tcgaagcctc
     5941 gatcctgctg atccttgtgg ttggcctggt ctactactac gcgaccggtc agggccggcg
     6001 cgaggacaag gtcgaggcgg acctcgccac gggcgaggcg atgatcgcct agacaggtcg
     6061 acatgcgccg cgcccgaccc ggggcggtgc ttcatcacga gagcgagaac gacggacggc
     6121 gcgtcgggca ggcccgacgc gccgtcccga ttcgggcatg atgcgagcgg aggcgcgacg
     6181 accgatgccc ggtgcgactg cgctcgagga ggcccgtcga tgacgaccca gcggggtgcg
     6241 ctcgaggacg agctgcgcgg cgaactccgc gacgagctag cccgtgcgtt gcaccggctg
     6301 cccgagctgg gaatgggcgg gctgaccttc acggcgctgt cgggcggcat caccaatcgc
     6361 aacttcctgg tcagcggcgc gcccggcggc gcgcgattcg tcgtccgact cgccggcaac
     6421 gacacgcacc tcctcggcat cagccgcgag gtcgagcacg cggcgaccgt gaccgcggcg
     6481 gggctcgggg tcgggcccga ggtcatcgcc tacctccgac ccgagggcta cctggtcaca
     6541 cggttcatcg agggcggccc gatcgctgaa gccgagatgc ggcagccggc gcgactgcgc
     6601 caggtgggcg ccacgctgcg ccgaatccac gacggaccgg cgatcccagg gctgttcgtg
     6661 ccgttccgga tcgtcgagtc gtatcgcgcc ctcgcgatct cacgcaacgt gccgatcccg
     6721 ccggagttcg atctcgccca atcgatcgcc cgccggatcg agcttgcctg cctgatggcg
     6781 ccgatcgaga tgcggccgtg ccacaacgac ctgctcaacg cgaacttcct cgacgacggg
     6841 tcgcgcatcc ggatcgtcga ctgggagtac gccgggatgg gcgacccgta cttcgacctc
     6901 ggcaacttca gcatcaacca cgagctcgac gccgaccagg acgcgatcct gctcgcggcg
     6961 tatctcgggc tcgatgcggg tgcagttgca ccgcgcccgc ttgcggcccg gcttacgctc
     7021 atgcgcgtcc tgtccgactt ccgcgaggcg atgtggggcg tgctgcagca gggctgctcg
     7081 acgctcgacg tggatttcgt cgcgtacgcc gccgagcact ttgaccgatt gctgaccgtc
     7141 gccggccggc ccgcgtttga gcgagccctc accgacgcgg cgggcggcta gggatctggt
     7201 ccgacagagc tcggccgggt cggccatcat cgcgcggtgg ctgaccaggc gcgcgtcgtc
     7261 gtcatcgggg gcgggatcac gggcgtgagc gtcgcgtatc acctcgccga ggcgggctgg
     7321 actgacgtgc tgctcgtcga gaaggccggt ttgaccgccg gctccacttc gcaggcggcc
     7381 ggactcgtga ccgcattcaa cccgtcatcg acgatgctcg cctggcgccg ccattcgatc
     7441 gagctgtacg gccggcttgg cgtgttcgac gccgttggct cggtccggct ggcatcgtca
     7501 ccggagcagc tgaaggagct cgagcgcacg gcgagccggg caaggggcgt cggcctcgac
     7561 gtcggggtca tctcggccga ggaggcgcgg cggctcttgc ctgcgatctc gcctgacaac
     7621 ctgtacggcg cggtgtacct gcccggcgac gggtacctcg atccgcacac ggcgacgttt
     7681 gcagttgccg atgcggcgcg gaagctcggc gtgcggatcc gccagaacac gcgggtcacc
     7741 gggttcgaca cggggctcgg gcccaggcgc gagatccgcc gcgtgctact ggagaacggc
     7801 gaggccatcg atacggagct cgtcgtcaac gcagcgggaa tgtgggcgcc tcaggtcgcc
     7861 gagatggtcg gcgcgttcat cccgtcgacc cccgtcgacc atcagcacgt cgcgctccgg
     7921 gccgtgccgg gccacgagct gccgaacgac atgcccgcgt tccgcgaccc ggacaacctc
     7981 gtctacggca aggccgagca cggcgggatg ctcttcggcg gctacgagaa cgcgccgcac
     8041 gcgcgctggg agacgggcgt tccgtgggac cacgccgcga cgtcgctccc accggattgg
     8101 gatcgattcc aaccgctcat ggagggcgcg atccggcgtt tcccgttcct cgccgacgcc
     8161 gaggccgtgc gcctcgtctg ccacccggac gcgatgacgc ccgatgccaa cccgctcatc
     8221 gggccgatgc ccggcgtgcg cgggttctgg gtcgctgccg ggctgtcgct gaacggcttc
     8281 ggcggtgggg gcgggatcgg gaaggcgctt gccggctgga tcacgagtgg cgatcccggc
     8341 gtcgacatcg cgccgtatcg agcgtggcgg ttcgccgaca cgtaccgcga cccggagtgg
     8401 acgtcgggtc tcgcccgcga gacgtacgcc gactactacc gcctgcggtt cccgtacgac
     8461 gctgacgtcg cggggcggcc gcgccgcctg tccgcgttgc accagcgtct cggacaggcc
     8521 ggcgcggtct tcggcacgaa ggccggctgg gagcgcgccg acctccatca gccggggcag
     8581 cgctggaccc gcaacgggcg gctgccacgg gagcgcgggt gggcacggcc cgactggttc
     8641 gagcgggtgg gggaggagca ccgcgcggtg cgcgagcgcg tcgggatcat cgacctctca
     8701 tcgttcggca agatcgcggt cgcggggccg ggcgcgctgg cgctgcttca gcgcgtcgcg
     8761 gcaaacgaca tcgaccggcc gatcggctcg acgatctaca cacagttcct cgacgagcgc
     8821 ggcgggatcg tcgccgatgt cacggtcacg cgcgactaca tcgaccgatt ccaggtcgtc
     8881 accggctccg ggtacctcgc cgcggacctg ggttggctcg tggcgcgggc ggccgatgag
     8941 ccggcgatcg acggcgtgac gatcgacgac cgaacggatg cgtggtcggt catcgggctg
     9001 tggggccctc gcgcccgcga ggtgctcgcc gggatcacga aggacgcggt cgatgacgcg
     9061 tcgctgccga ttcggcaggc gcgctcgatc cgcgtcggcg ggttcggcgt caccgcgacg
     9121 cgcctgagct acgcgggcga gctcggctgg gagctgctgg tcgagccggc gaacgcggtc
     9181 gaggtctggg acggcctgct cgcgtcgggg aggccgcagg gcatcgagcc ggtcggctac
     9241 cgggcgctcg aatcgctccg gctcgagaag ggctaccgct acttcggcgc cgagctgacg
     9301 ccgcgcgaaa ccccgttcga ggccgggctc gagcggttcg tccgcttcgg caagggcgac
     9361 ttcgttgggc gcgacgcgct cctcgcgcgt cgggcggccg agccggatgg ccctgcgcgg
     9421 cgtctccgaa ccgtgctcat tggcgggagc gactgggcgc cgatctacgg cggcgaggct
     9481 gtccgcgtcg atggcgaggt ggttggccgg ttgcggagcg ccgcgttcgg ctacacggtc
     9541 ggacgcaccg tcggaacgac ctatctcccc gctcgcatcg ccgagggcgc cgccatcgag
     9601 gtggatgtct tcgccgatcg aatcgcaggc gaggtggccg cggatgtcct cgtcgacgcg
     9661 gccggcgacc ggatgaaggc ctagccgcag gaagggtcag cccggcttcc gcgcgagcgt
     9721 gtagccccac cggacgtgct cggtgaacct cgtcgaatcg tcagctccga gcgtggatcg
     9781 cagctcgtcg atccagacgt cgaaatcggg tccgatcgcc ttcctggagc agcccgaggt
     9841 ggacgccagg tagccgacga gctcgtcgaa gctgagctgc acggtctgct cgaagtcgtg
     9901 gccttggatg tcgacgaatc cggctgatcg catggccgcc tcacgatccg cctgatcgag
     9961 tgcaccgccc ggcagcacga ggccgaaacc ctccggaaac gaccgagcga tccagcgatc
    10021 gcggacaacg cgatacgtga cctcccacgg ctcgtcgccc gagaaccggt tgtcggtgcc
    10081 gagggttgcg aggcagccgc cgggtcggag ccagcggaac acgttgcgag cgacgactgc
    10141 cgcctcgagg cgatggaacg cctcgccgat cgtcacgaga tcgaacgatt cggtctgagc
    10201 ctcgaaggtc tcgacgcggc cgacgtgcca ggtgacggtc ttgatgtcgc gacgcgctgc
    10261 ctcgcgtcgt gcaacgtcga tcatgtccgg ctcgaggtcg actgcgacga cgcgggcaaa
    10321 gcgcggcgcg aggtcgagcg cgatccggcc cggcccggtc gccaggtcga gcagcgcctc
    10381 ccgcccgacc gccgcccgat cgagcaggtc ggcgagcacg tgcggcggat acggcggccg
    10441 gtaccgggca tacgcctcgg ccgcaccggc aaaggcgccg ggccgaaggt tcgcaggaat
    10501 gccggcgagg tcagccatcg attcgtccaa ggtcagccct cgctcgcggc gcccatgagg
    10561 tcgtcgaagt cgggtcggtc gagcgccgtc gaggcgcggc cccagcgctc ctcggcccag
    10621 ccccagaagt cgtagtcgat cgtcgagatc gcggcctgga tggcggccca gagcgtccag
    10681 ccgacgtcgg acatgatcat ctggaggcgc atccgggcca ccagcgcccg gccctccagt
    10741 gcgaatcgct cgggaccgaa gtacgccgcg cagagctcct cctgtcgcgc ggggtcgaag
    10801 ccgagctcct gggcggtgtt gcccagctcg aaggtcgggt cgttgttgcc gctgtactcg
    10861 tagtcgacga tccacagctt cgaaccgtca tcgaggtagt tctcggcgag caggtcgttg
    10921 tggcaaggca cggtcgcgag cggctgggcc gcgagcgccg cctcgatccg gggaagctgg
    10981 tcgaggtgtt cgcgatagcc gtcggggatg cggatcgagc gctcgtcgac gacgcgcagg
    11041 tagaactcgg tgagccggaa catgtcgaag gcgtcgcgga agcgcgggcc ggcatggagc
    11101 cgccgaaggg cctcggcgat ccgcgcggga gcccccggct cggcgaaggc ggtgttcgac
    11161 atcgtccgac catcgatcca ctcgagcgcg aacgcgcctg tttctggatc gacctcgacg
    11221 accttcggtc cgactccggc ctccgccgca gcgcgtgtgt tgtgcagctc gttcgcgcga
    11281 tcgaccgcga gcaggtcggt cgaggctccg gggaggcgca cgaagcaggg ccggccgtcg
    11341 acctcgacgc ggaagttctg gttcgtcagg ccggcggcga tcggagagac ctcgacgtcg
    11401 cgcccgggcc acaggcgtgc gaggaccggc tcgatgccgg ggcgcgcagg gtccatcacg
    11461 tggcggccgc cgccgggttc ggcgccgcgg catccggcgc cgccgcagcc ggggccgcgg
    11521 attccgggtc ggtgtcgact tccgcgggcg cgtcgcgggc ggtcgtgtcg ccgagtgcgg
    11581 actgggcgtc ccgacgctcc gcccagacga gcgcaacgga cacgagcgtc aggacgatga
    11641 atccgagtga gccggtcagc cccgagttgc cgctcagctc catcggcacg tggaacatcc
    11701 gcgcgaccga ggcggaagcc gtgacgttcc acaggacaag gaagcccagg acggccgaga
    11761 acgcgaccat ccggcggcgg tccggcagga gcgccatgaa gacgatcgcg gcgtaggaat
    11821 agcggtcgtg catggtcgtg aggaaggtga acgcgatgag cgtcgacgcg gccaggctga
    11881 gcgcgagcac gaccggcgtc ggccggcgcc agacccggta cgcgacgagc gccgcgaggg
    11941 cgaccgtgat caggtagccg gccatgcggt acgtcagtgg accgacgagc gagtcttcgt
    12001 cgggcaccca cgagacgtgg aggatgctcc gctggacgat ccaccagacg ttccacgccc
    12061 gcatcgagat cacgtcgaac cgctcgtcgt ggtagtaggc gagccatccg aggtacttgc
    12121 tcggaccgtc ccattggagg aagggcagcc agagcgcgat cgcgacgacc accgcggtca
    12181 tcccggcccg gatgaccgtg cggcgatcgt acgtggcgag ataccaggcg gcgaacggaa
    12241 gtgcaagcgc cagggcctgg ggcttcgtca ggatcgccgc cgtgatcgcg atcgccgcga
    12301 gcagtggtcg atcgcgcagc acgaggacga acgcgagtag ggcgaa
//
LOCUS       DATBSW010000170         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_192094, whole genome shotgun sequence.
ACCESSION   DATBSW010000170 DATBSW010000000
VERSION     DATBSW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192094"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VIF63_04760"
     CDS             <1..518
                     /locus_tag="VIF63_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY8238726.1"
                     /translation="RSLLDHVLDAVAEVTDVTVLVLAPDETPAGIPSRVRLAHDRDAY
                     AGPLAGLAAGLEAAHDADLALVVGADMPSLQPEVLRLMLDRAGEPGGPDAWTLEGPDP
                     SLVGPLPLAGRAAVLRAAATQLVIDGEHSLRSLVGKLGAGRIAAADWRPLDPEALTLR
                     DVDRPGDLPPG"
     gene            complement(525..>2061)
                     /locus_tag="VIF63_04765"
     CDS             complement(525..>2061)
                     /locus_tag="VIF63_04765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8238727.1"
                     /translation="ALGTAGAVGALRGPATRVIELRGRTVSPGFQDAHCHPVYAGMDL
                     IHCALHDYYRADEYLEAIAAYAAAHPADPWIVGSGWSMGSFEGGYPDRQLLDRVTGER
                     PAFFDNRDGHDAWVNSAALRLAGIDRDTPDPVDGRIRRDANGDPTGALHEGAMRLVTT
                     ILPDDKDADFDRALLIAQEQLHSLGITAWQDAIVQPRTEAIYRTAAARGDLTARVIGA
                     LWWDRERGLEQIEELVERRDRGPVGRFSPTSVKLMLDGIIENGSAKLTAPYLDEDGQP
                     TDKSGLDFIEPERLNVAVSKLDALGFQCHFHAIGDGAVRRGLDASEGALAANGRTDGR
                     HHISHIQVVHPDDVPRFGRLGVVANAQPLWACSSEQMVKLNNSVLGPERTTWQYPFAS
                     LLRTGATLAMGSDWAVSSANPLEEMDVAVRRVDPHGFDPEPFLPHEAISLDEALAGFT
                     RGSAYVNHLDETGLLAAGMLADLAVFDRDLHDPSAGSLSDARVVATAVGGAFVFEDAA
                     LEG"
BASE COUNT          292 a          717 c          745 g          307 t
ORIGIN      
        1 atcgcagcct cctcgaccac gtcctggacg cggtcgcgga ggtgacggac gtgaccgtgc
       61 tcgtcctcgc tcccgatgag accccggcgg gcatcccgtc ccgggtgcgc ctcgcccacg
      121 accgcgatgc atatgccggc ccgctggcag gcctggccgc cggcctcgag gctgcccacg
      181 atgcggacct agcactggtc gtcggcgcgg acatgccgtc gcttcagccc gaggtgctgc
      241 ggctgatgct cgatcgtgcg ggggagccgg gcgggcctga cgcgtggaca ctcgaagggc
      301 cggatccgag tctcgtcggc ccactgccgc tcgcaggtcg tgcggccgtc ctccgagcgg
      361 ctgcgacgca actggtgatt gacggcgagc actcgctccg aagcctcgtc gggaagctcg
      421 gcgccgggcg gattgcggcc gcggattggc gcccgctgga ccccgaagcg ctgacgctgc
      481 gcgacgtcga ccggcccggg gacctgccgc ccggctaacc cgggctaccc ctcgagcgcc
      541 gcgtcctcga agacgaaggc gccgccgacc gccgtcgcga cgacgcgcgc gtcagacagc
      601 gagcccgccg agggatcgtg gaggtcgcgg tcgaagaccg caaggtcggc cagcatcccg
      661 gccgccagca ggcccgtctc gtcgaggtgg ttgacgtacg cggagccgcg cgtgaagccg
      721 gccagcgcct cgtcgaggct gatcgcctcg tgcggcagga atggctcggg atcgaagcca
      781 tgcgggtcca cgcgccggac cgcgacgtcc atctcctcga gcgggttcgc cgatgaaacc
      841 gcccagtcgg agcccatggc cagcgtggcg ccggttcgaa gcagcgacgc gaacgggtac
      901 tgccaggttg tccgttcggg cccaagaacg gagttgttga gcttgaccat ctgctcgctg
      961 ctgcaggccc acaggggttg cgcattcgcg acaacgccga gacgaccgaa tcgcggtacg
     1021 tcgtcggggt ggaccacctg gatgtgcgag atgtggtgcc ggccgtccgt tcgaccgttg
     1081 gcggcgagcg cgccttcgga ggcatcaagg ccccgccgca cggcgccgtc gccgatcgcg
     1141 tggaagtggc actggaagcc gagcgcatcg agcttcgaaa cggcgacatt gagccgctct
     1201 ggctcgatga agtcgagacc gctcttgtcg gtcggttggc catcctcgtc caggtacggc
     1261 gcggtgagct tcgcggaacc gttctcgatg atcccgtcga gcatgagctt caccgacgtc
     1321 ggcgagaacc gcccgaccgg cccgcgatcc cggcgttcga cgagctcctc gatctgctcg
     1381 aggccccgct cgcgatccca ccacagcgcg ccgatgactc gcgccgtcag gtcgccacgg
     1441 gcggcggccg ttcggtagat cgcctcggtt cgcggttgca cgatcgcgtc ctgccaggcc
     1501 gtgatcccaa ggctgtgcag ctgctcctgg gcaatcagga gcgcgcggtc gaagtcggcg
     1561 tccttgtcgt cgggcaggat ggtcgtgacg agccgcatcg cgccctcgtg aagggcgccc
     1621 gttgggtcac cgttcgcgtc gcggcggatg cggccatcga cggggtccgg cgtgtcgcgg
     1681 tcgatccccg cgagccgaag ggccgcgctg ttgacccagg cgtcgtggcc gtcgcgattg
     1741 tcgaagaatg ccggccgctc gccggtcacc cggtccagga gctggcgatc ggggtagccg
     1801 ccctcgaagg agcccatcga ccagccactg ccgacgatcc aggggtcagc cgggtgggcg
     1861 gccgcgtacg cggcgatcgc ttcgaggtac tcgtcggccc ggtagtagtc gtgaagggcg
     1921 cagtggatga ggtccatgcc ggcatagacc ggatggcagt gggcgtcctg gaatccgggg
     1981 ctgacggtcc gaccgcgaag ctcgatcacg cgcgtcgccg ggccgcgcag cgcgccaacc
     2041 gcgcccgctg tcccgagcgc c
//
LOCUS       DATBSW010000171         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1179577, whole genome shotgun sequence.
ACCESSION   DATBSW010000171 DATBSW010000000
VERSION     DATBSW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179577"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VIF63_04770"
     CDS             complement(<1..534)
                     /locus_tag="VIF63_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEY8238728.1"
                     /translation="MVPARRRVAPRRRVVELGDADGQAAAGVHVSASSSHGGEGRHRP
                     SNDLSSSAQEYLLTLRIMAGDGSRITASQVARKLGVSTQAASEMFRRLASDGLVTLAE
                     GRELQLTKPGRTAADGIFHRHALCEWLLTEIVGLGWAESDAEAERLQAAISPRVEAQL
                     DELLGHPETCPHGNPLDA"
     gene            complement(444..1796)
                     /locus_tag="VIF63_04775"
     CDS             complement(444..1796)
                     /locus_tag="VIF63_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8238729.1"
                     /translation="MPAQPNPAADPYRTLGLEPGASMAEVKRAYRLLAKAFHPDSAGE
                     AALPRFLAIHEAYEQLKTGRAPAAGRASKTSAAPAAEPWRADPARARAAREKARTARG
                     SSSRAGAGTAGATGAGRSRTGAGATGAPSAAGASSKSKSSSSSADASATGGSTRGGRR
                     STRKATMGSTSYDEARDPADASWSGASWYGPSSGEYWIVNPREYADPRKHGPDYQQRA
                     RRLAESDAFEPVAAASSTAGTDDEARTDQQAASGTDDSYRAAWAAANARPATPRPTVW
                     TSDAGARRPGDGRREATTDASLGTGDAPPVEQLAAASRAWLGGPADDPVRRLGLALVA
                     WPPIGLVAASAIGEATGCAAFSAECGGSEPLLPWLAQAVILGLLLLLPPVTRALAGGS
                     IAVLLALVPITAFVLAVGGGGAPQAALVVWFLLGVAWLLGVAWSSWVTLTGRRPRVST
                     "
     gene            complement(1825..2937)
                     /locus_tag="VIF63_04780"
     CDS             complement(1825..2937)
                     /locus_tag="VIF63_04780"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8238730.1"
                     /translation="MAAPSAAKPRRTAPPVLVRQVRNGVEESVHRGDIVEVDISGHML
                     RALGEPDHVVNLRSAVKPFGLMALLEAGGEREFELQPAEIAVMAASHSGEDLHVRTLQ
                     AVFRRTGVSQSGLVLNTEGAPIDALTAARLARDGERPGEIRHMCSGYHASFLLLARLG
                     GWSADGYWLDDHPVQVAARDAVAGAFGTTSAKLVASIDACGVPAYAFKLREVARAYAF
                     LADPESVPASDSRSALATSMRVVRDAMLAHPEMIGGTRDRLDTSVAKALPGRLAAKGG
                     AEGLRCFAILPGPRGRGGFSAATGMALKIEDGGSADRATSAASIEALAQAGVLEGQSL
                     RMLGLYHRPTKADPHGRPAAEAIASFELAPLGELLR"
     gene            3019..3651
                     /gene="upp"
                     /locus_tag="VIF63_04785"
     CDS             3019..3651
                     /gene="upp"
                     /locus_tag="VIF63_04785"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8238731.1"
                     /translation="MLHVSNHPAVLHKLAILRDEKTEPKKFREVVRELSWLLGYEALA
                     DARVRAREVRTPLETMSGHELGERIGLIPILRAGLGMVDAMLELMPTAEVWHLGLFRD
                     EKTLRPVEYYNKLPDSANVDLCLILDPMLATGGSATAAIEVLKRWGAVQPVRIKLLNL
                     IAAPEGVEAVAAAHPDVEIHCAALDRGLNEKGYIMPGLGDAGDRQFGTGK"
     gene            3684..>4494
                     /gene="chrA"
                     /locus_tag="VIF63_04790"
     CDS             3684..>4494
                     /gene="chrA"
                     /locus_tag="VIF63_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY8238732.1"
                     /translation="MPPADASPARIFGRFLRFGVLAFGGPVAQIAMLRRELVDEERWI
                     ATERFNRLLAVYQVLPGPEAHELAVHLGFLRGGRLGGLLAGLGFMLPGFSLMFGLSWL
                     YFQVDLTQAAIGAALLGAQIAVIALVVRAVVRLSEHILVDRGLVVIAVAGGIATLAGV
                     SFWIVLPVAGLAHVLVPRAPRGSVHLIVLGAAAAALVAAVASTGAFALPGPVVGPPSP
                     SAFGDPSLAEIFGSGLKAGLLTFGGAYTAIPFLRGDAVGSGWLTDAQFLDGI"
BASE COUNT          587 a         1612 c         1637 g          658 t
ORIGIN      
        1 ggcgtcgagc ggattgccat ggggacacgt ctcgggatgg ccgagcagct cgtcgagctg
       61 ggcctcgacc cgcggcgaga tcgcggcctg caggcgctcg gcctcggcgt cggattcggc
      121 ccagccgagc cccacgatct ccgtgaggag ccactcgcac agggcgtgcc ggtggaagat
      181 gccatcggcg gcggttcggc cgggcttggt gagctggagc tcgcggccct cggcgagggt
      241 cacgaggccg tcgctcgcga ggcgccggaa catctcgctg gcggcctgcg tgctcacccc
      301 gagcttccgg gcgacctgcg acgcggtgat ccgcgagccg tcgccggcca tgatccgaag
      361 cgtgagcagg tactcctggg cggagctgga gaggtcattg gatgggcggt gtcgaccctc
      421 gcctccgtgg ctgctcgagg cgctcacgtg gacacccgcg gccgcctgcc cgtcagcgtc
      481 acccagctcg accacgcgac gccgaggagc cacgcgacgc cgagcaggaa ccacaccacc
      541 agtgcggctt gcggcgcacc gccgccaccc acggcgagca cgaacgcggt gatcgggacg
      601 agcgcgagga gcacggcgat cgagccgccg gcgagcgctc gggtgaccgg tgggaggagg
      661 agcagcagcc cgagaatgac cgcctgggcg agccacggca gcagcggctc cgatccaccg
      721 cactcggccg agaaggccgc gcagccggtg gcctcgccga tcgccgacgc agcgaccagg
      781 ccgatcggcg gccaggcaac gagtgccagc ccgagccgac ggaccgggtc gtccgcggga
      841 ccgccgagcc acgcgcgcga tgccgctgcg agctgctcga ccggcggtgc gtcgccggtg
      901 ccgagcgacg cgtcggtcgt ggcctcgcgg cggccgtcgc cgggccggcg ggcaccggcg
      961 tcggatgtcc ataccgtggg tcgcggggtc gccggtcgag cgttcgcggc ggcccacgcg
     1021 gcccgatagc tgtcgtcagt gcccgaagcg gcctgttggt cggtgcgcgc ctcgtcatcc
     1081 gttcctgctg tcgaagacgc tgcagccacc ggctcgaacg cgtcggattc ggcgaggcgc
     1141 cgcgcgcgct gttgatagtc gggcccgtgc ttgcgcgggt cggcgtactc gcgcgggttg
     1201 acgatccagt actcccccga cgacgggccg taccaggagg cgccgctcca tgacgcgtcg
     1261 gccgggtcgc gggcctcgtc atacgaggtc gagcccatcg tcgccttgcg cgtcgagcgc
     1321 cggccgccac gggtcgagcc gcccgtcgct gaagcatcgg cagacgatga cgaggatttc
     1381 gacttcgagg aggccccagc cgcgctcgga gcgccggttg cgccggctcc agtgcgcgaa
     1441 cgcccggctc cggtcgcgcc ggccgttccg gccccggccc tcgacgagct acccctcgcg
     1501 gtgcgcgcct tctcgcgggc cgctcgggct cgcgccgggt cggcgcgcca gggctcggcg
     1561 gcaggcgcgg ccgaggtctt cgaggctcgc cctgcggcgg gcgcccggcc cgtcttcagc
     1621 tgctcgtatg cctcgtggat ggcaaggaat cgtgggagcg cggcctcgcc ggccgaatcg
     1681 ggatggaacg ccttggcgag gagccgatac gcgcgcttga cctcggccat cgacgcgccc
     1741 ggctcgaggc cgagggtccg gtacgggtca gcggcggggt tgggctgtgc gggcacgacg
     1801 caagcgtatg ccgaccgcca cgggtcagcg cagcagctct ccgagtggcg cgagctcgaa
     1861 cgacgcgatg gcctcggcag caggtctgcc atgcggatcg gccttggtcg ggcggtgata
     1921 caggccgagc atccgcagcg actggccctc gagaaccccg gcctgtgcca aggcttcgat
     1981 cgatgcggcg gacgtcgcgc gatccgcgct gccgccatcc tcgatcttca acgccattcc
     2041 ggtggcagcg gagaagccgc cgcgcccacg cggcccgggc aggatcgcga agcagcgcag
     2101 gccctcggcg ccccccttcg cggcgagccg gcccggcagg gccttcgcga cggaggtgtc
     2161 gagccgatct cgcgtgcccc cgatcatctc cgggtgggcg agcatcgcgt cccggacgac
     2221 tcgcatcgac gttgccagcg ctgaccggga gtcgctcgcg ggaaccgatt cggggtccgc
     2281 gaggaatgcg taggcgcgag cgacctcccg gagcttgaag gcgtaggccg ggacgccgca
     2341 cgcgtcgatg ctcgcgacga gcttcgccga ggtcgtcccg aaggcgcccg ccacggcgtc
     2401 ccgcgccgcg acctgcacgg gatggtcgtc gagccaatag ccgtccgccg accagccgcc
     2461 gagccgcgcg agcagcagga acgaggcgtg gtagccggag cacatgtgcc gaatctcccc
     2521 gggccgctcg ccgtcacgtg ccaggcgcgc cgccgtgagc gcatcgatcg gcgcgccctc
     2581 ggtgttcagg acgagccccg actggctcac cccggtgcgc cggaagactg cctggagcgt
     2641 gcggacgtgc aagtcctcgc cggagtggct cgcggccatg accgcgatct ccgccggctg
     2701 gagctcgaac tcacgctcgc cgccggcctc gaggagcgcc atcaggccaa acggcttgac
     2761 cgccgaccgg aggttcacga catggtcggg ttccccgagg gccctgagca tgtgccccga
     2821 gatgtcgacc tcgacgatgt cgccgcggtg gacgctctcc tcgacgccgt tgcggacctg
     2881 tcgaacgagg accggcggcg ccgtcctgcg cggtttcgcg gcggacgggg cggccacggc
     2941 gatacggtcc tccaggtgcg ccgcacacgg gcgggcgtgt cggcaaacgg tactccgccc
     3001 gatgctagga tgccgatcgt gctgcacgtc tcgaaccatc ccgccgtgct ccacaagctc
     3061 gccatcctgc gcgacgagaa gaccgagccg aagaagttcc gggaggtcgt gcgcgagctg
     3121 tcctggctgc tcgggtacga ggcccttgcc gacgcacggg ttcgcgcgcg cgaggtgcgg
     3181 acgccgctcg agacgatgtc cggccacgag ctcggggagc gcatcgggct catcccgatc
     3241 ctgcgcgcag gcctgggcat ggtcgacgcc atgctcgagc tgatgccgac cgccgaggtc
     3301 tggcacctgg gcctgttccg cgacgagaag acgttacgac cggtcgagta ctacaacaag
     3361 ctgcccgact cggcgaacgt cgacctgtgc ctgatcctcg acccgatgct cgcgaccggc
     3421 ggctccgcca ctgcggcgat cgaggtcctc aagcgctggg gcgcggtcca gccggtgcgg
     3481 atcaagctgc tgaacctgat cgcggcgccg gaaggcgtcg aggccgtcgc ggcggcccac
     3541 cccgacgtcg aaatccactg cgccgcgctc gatcgtgggc tgaacgagaa gggctacatc
     3601 atgcccggcc tcggtgacgc cggcgaccgc cagttcggga cgggcaagta ggcggagcag
     3661 cgattcccgc tccggcgtcc gggatgccgc ccgccgacgc ttcaccggcg cgaatcttcg
     3721 gtcggttcct gcgcttcggc gtcctcgcct tcggtggccc ggtcgcacag atcgcgatgc
     3781 tccggcggga gctcgtggat gaggagcgct ggatcgccac agagcggttc aatcgcctgc
     3841 tcgctgtcta ccaggtgctg ccggggccgg aggcccacga gctcgctgtc cacctcgggt
     3901 tcctgcgcgg cgggcgcctc ggcggtctgc tcgccggact cggcttcatg ctcccgggct
     3961 tctcgctcat gttcggcctg tcctggctgt acttccaggt cgacctgacc caggcggcga
     4021 tcggtgcggc gctgctcggc gcgcagatcg ccgtcatcgc gctcgtcgtt cgagccgtgg
     4081 tccggctctc cgagcacatc ctggttgacc gtgggctggt ggtcatcgcg gttgcgggcg
     4141 gcattgcgac cctcgccggc gtgtcgttct ggatcgtcct cccggtcgcc ggcctggccc
     4201 acgtgctcgt gccacgggcg ccgcgaggct ccgtccatct gatcgtcctc ggtgccgccg
     4261 cggctgccct ggtcgcggcc gtcgcgtcga ccggcgcgtt cgcgctacca ggtccggtgg
     4321 tcgggccacc ttcgccgtcg gcatttggtg acccgtctct cgccgagatc ttcgggtccg
     4381 gcctcaaggc cgggctgctc acattcggcg gcgcctacac cgcgattccg ttccttcgtg
     4441 gtgatgcggt cggcagcggc tggctcacgg atgcccagtt cctcgacggg atcg
//
LOCUS       DATBSW010000172         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1179641, whole genome shotgun sequence.
ACCESSION   DATBSW010000172 DATBSW010000000
VERSION     DATBSW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179641"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VIF63_04795"
     CDS             <1..295
                     /locus_tag="VIF63_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8238733.1"
                     /translation="LKKAENLTVDHGALIGPSTDADGATQPAITPGSPAEAAGLKDGD
                     IILSIEDQQIDAEHPLDAVLTSYRPGQTVKVTILRGTEQSVLSVTLGTRPEGL"
     gene            complement(299..667)
                     /gene="folB"
                     /locus_tag="VIF63_04800"
     CDS             complement(299..667)
                     /gene="folB"
                     /locus_tag="VIF63_04800"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8238734.1"
                     /translation="MSDRVFLAGMAFQARHGVLDWEKVEAQRFEVDVELVLDVQPAGL
                     DDDLTRTVDYGGVYGIVRQVVESTTFNLIEALAEAIAHEVLGANERVDEVVVRVRKPD
                     VRLDGPLAYAGVEIRRVRER"
     gene            complement(664..1569)
                     /gene="folP"
                     /locus_tag="VIF63_04805"
     CDS             complement(664..1569)
                     /gene="folP"
                     /locus_tag="VIF63_04805"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8238735.1"
                     /translation="MSERRDLGETRIGPRTFRWGERTFVMGILNVTPDSFSGDGLVTA
                     AGEAGEAGEAGATAAHVARAVAQARAMAGEGADLLDIGGESTRPGHAEVDEAEELRRT
                     IPVVAAVRAALPDIPITIDTTKPAVAAAALDAGANAVNDIWGVGPDSALTGLAAERGV
                     PIIVMHNRREPHYEDLVAEVVNDLARAIERALGAGVAPTDIIVDPGIGFGKTAEQNLA
                     LLRDLGELRSLGRPILLGTSRKSTLGKVLDLPVEERLEATLATTALGIGAGVDIVRVH
                     DVRQNVRAARMADAIIRGTWREVRA"
     gene            complement(1566..>2754)
                     /locus_tag="VIF63_04810"
     CDS             complement(1566..>2754)
                     /locus_tag="VIF63_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8238736.1"
                     /translation="VLALASSALRAAGIRTGATPKPHLVSYRERLEIDGRPIDPVTFT
                     RLVGEVLEVAGGVARRHGQPTEFELLTAMLFRWFAEERVEVALVEVGLGGRLDATHAW
                     DGGVAAITNVALDHMAWLGDTIPAIAREKAAIIERGDIAVTGAGGEALPVIRRRAHRV
                     GAPLTEVGAAPILGWDRDGIDVELGALGPTRISLRGRHQAENAAVADAVLDALGTAGI
                     ATVPAEARRRGYATAEWPGRLEILTVQGREVVLDGAHNPAGAAALAQALDDLRPFLYR
                     GHDASPPPLTLVHGSMADKDVDGIIGALDRAGALRGARIIATQVPGDRALPAADLARR
                     WRAARPDAAVTVVVDVGAALGRALAEADGPVVVAGSLYLVGEARGRWLDDPRLRDPEM
                     PTT"
BASE COUNT          374 a         1015 c          998 g          367 t
ORIGIN      
        1 gctcaagaag gcggagaacc tgaccgtcga ccacggggcg ctgatcgggc cctcgaccga
       61 cgccgacggc gccacgcagc cggcgatcac gccgggcagc ccggcggagg ccgcgggcct
      121 caaggacggc gacatcatcc tgtcgatcga ggaccagcag atcgatgccg agcacccgct
      181 cgacgccgtc ctcaccagct accggcctgg ccagacggtg aaggtcacga tcctgcgtgg
      241 cacggagcag tcggtgctga gcgtgaccct gggaacgcgg cccgaaggac tgtagggttc
      301 agcgctcccg gacccggcgg atctcgacgc cggcgtaggc gagcggtcca tccaggcgca
      361 cgtcgggctt cctgacccgc accacgacct cgtccactcg ctcgttcgcc ccgagcacct
      421 cgtgggcaat tgcctcggcg agcgcctcga tcaggttgaa cgtcgtcgac tcgaccacct
      481 gcctgacgat cccgtagaca ccgccgtagt cgaccgttcg ggtcaggtcg tcgtcgagcc
      541 cggcgggctg cacgtcgagc accagctcga cgtcgacctc gaaccgctgc gcctcgacct
      601 tctcccagtc gagaacgccg tgtcgagcct ggaacgccat gccggcgagg aagacccggt
      661 cgctcaagcg cgcacctccc gccacgtgcc gcggatgatc gcgtctgcca tccgggcagc
      721 gcggacattc tggcggacgt cgtggacgcg cacgatatcg acgcccgcgc cgattccgag
      781 cgccgtggtc gcgagggtcg cctcgagccg ctcctcgacc gggaggtcga gcaccttgcc
      841 gagcgtggat ttccgcgagg tgccgagcag gatcgggcgg ccgagcgacc gcagctcgcc
      901 gaggtcgcgc aggagcgcga ggttctgctc ggccgtcttg ccgaacccga tgccggggtc
      961 gacgatgatg tccgtcgggg ccacgcccgc tccgagcgcg cgctcgatcg cccgggcgag
     1021 gtcgttgacg acctccgcca cgaggtcctc gtagtgcggc tcgcgccggt tatgcatgac
     1081 gatgatcggg acgccacgct cggcggccag cccggtgagc gcgctgtcgg ggccgacacc
     1141 ccagatgtcg ttgaccgcgt tggctcccgc gtcgagtgcg gccgcggcga cggccggttt
     1201 cgtcgtgtcg atcgtgatcg ggatgtccgg cagcgccgcg cggacggccg cgaccaccgg
     1261 aatggtccgc cggagctcct cggcctcgtc aacctcggcg tggcccggcc gcgtggattc
     1321 gcccccgatg tcgagcagat cggcgccctc gccggccatg gcccgggcct gcgcaacggc
     1381 gcgcgcgacg tgcgccgcgg tggcgccggc ctcgccggcc tcgccggcct cgccggccgc
     1441 ggtgaccagg ccgtcgcccg agaacgaatc cggcgtcacg ttgaggatgc ccatcacgaa
     1501 cgtgcgctcg ccccagcgga atgtccgcgg gccgatccgc gtctcgccga ggtcgcgccg
     1561 ttcgctcatg tggttggcat ctccgggtcg cgcaatcgag ggtcgtcgag ccagcgaccg
     1621 cgtgcctcgc cgacgaggta cagcgagccg gcgacgacga ccgggccgtc ggcctcggca
     1681 agggctcgac cgagcgccgc gccaacgtcg acgacgaccg taaccgcggc gtcgggccgg
     1741 gccgctcgcc aacggcgcgc caggtcggcc gcgggcaagg cccggtcgcc tggcacctgg
     1801 gtggcgatga tacgagcgcc gcggagtgcg cctgcccggt ccagggcgcc gatgatgccg
     1861 tcgacgtcct tgtcggccat cgacccgtgg acgagggtca gcggcggggg cgatgcatcg
     1921 tggccgcggt agaggaacgg tcgcaggtcg tcgagcgcct gggcgagtgc cgcggcgccc
     1981 gccgggttgt gggcgccatc gaggacgacc tcccgacctt ggaccgtgag gatctcgagg
     2041 cgaccgggcc actccgccgt cgcgtagccg cggcgtcggg cctcggccgg gacggtcgcg
     2101 atgccggcgg tccctagcgc gtcgaggacg gcgtccgcaa cagccgcgtt ctccgcctgg
     2161 tgccggccgc ggagcgagat ccgggtcggc ccgagcgccc cgagctcgac atcgatcccg
     2221 tcgcgatccc agccgaggat cggcgccgcg ccgacctccg tcaggggcgc gccgacccgg
     2281 tgggcgcgcc gccggatcac cggcagcgcc tcgccgccgg cgccggtcac ggcgatgtca
     2341 ccgcgttcga tgatcgccgc cttctcgcgc gcgatcgccg ggatcgtgtc gccgagccag
     2401 gccatgtggt cgagcgcgac gttcgtgatg gccgcgacgc cgccgtccca ggcgtgggtc
     2461 gcgtcgagcc ggccgccaag cccgacctcg accagcgcga cctcgacacg ctcctcggca
     2521 aaccagcgga agagcatcgc cgtgagcagc tcgaactcgg tcggttggcc gtgtcgcctg
     2581 gcgacacccc ctgcgacctc gaggacctcg ccaacgagcc gggtgaacgt caccgggtcg
     2641 atcggccggc cgtcgatctc cagccgctcc cgatagctca cgaggtgcgg cttgggcgtg
     2701 gcgccggtcc ggatgccggc ggcgcgcagg gcgctcgagg ccagggcgag gacg
//
LOCUS       DATBSW010000173         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_509710, whole genome shotgun sequence.
ACCESSION   DATBSW010000173 DATBSW010000000
VERSION     DATBSW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509710"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="hydA"
                     /locus_tag="VIF63_04815"
     CDS             <1..1093
                     /gene="hydA"
                     /locus_tag="VIF63_04815"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY8238737.1"
                     /translation="KGQTLREGMDAWHAKAEGNAVTDYGFHMIMSDVNDATLDEMDGL
                     VAEGVPDFKLFTAYPGVFYSDDGAIFRAMQQTAKNGGLIMMHAENGLAIDVVAGQAVD
                     EGHTDPYYHGTSRSPILEGEASNRVIRLAEAAGAPVYIVHLSARHALEEVRRARDEGL
                     PAFAETCPQYLFLTLEDMGNGFEGAKFVCSPPLRPADHAPELWKGLISDDLQVVSTDH
                     CPFDFHGQKEMGRGDFRKVPNGLPGVENRVDLMHDGGVVGGHLTPNRWVQLISTAPAR
                     LFGLPQKGAVAVGADADLVVYDPNRRHTISAASHHMDVDYSCYEGREVTGGSDVVLSR
                     GKLIVDGETWLGAKGDGRFLKRVRSEYLR"
     gene            1243..>1505
                     /locus_tag="VIF63_04820"
     CDS             1243..>1505
                     /locus_tag="VIF63_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238738.1"
                     /translation="MVGDPELEATLPDEAGDIRFYQTFSMSGPDFLSADIDDQFVRFV
                     ESLGADIDDVSVAFALGSNFDVTDTASVFAFQVDGAEAADLVD"
BASE COUNT          241 a          520 c          519 g          225 t
ORIGIN      
        1 caagggccag acgctgcgcg aggggatgga cgcctggcac gccaaggcgg agggaaacgc
       61 cgtcaccgac tacggcttcc acatgatcat gagcgacgtc aacgacgcga cgctcgacga
      121 gatggacggc ctggtcgccg agggcgtccc cgacttcaaa ctgttcaccg cctatccggg
      181 cgtgttctac agcgacgacg gcgccatctt ccgggcgatg cagcagacgg ccaagaacgg
      241 cgggctgatc atgatgcacg ccgagaacgg gctggccatc gacgtcgtcg ccggccaggc
      301 ggtggacgag ggacacaccg atccctacta ccacggcacc tcccgatcgc cgatcctcga
      361 gggcgaggca tcgaaccggg tgatccgcct ggccgaggcc gccggtgcgc cggtctacat
      421 cgttcacctc tccgcgcgcc acgccctcga ggaggtgcga cgggcacggg acgaggggct
      481 gccggcgttc gccgagacgt gcccgcagta cctgttcctc accctcgagg acatgggcaa
      541 cgggttcgag ggcgccaagt tcgtctgctc gccgccgctc cgccccgcgg accacgcgcc
      601 ggagctgtgg aagggcctca tcagcgacga cctgcaggtg gtgagcaccg accactgccc
      661 gttcgacttc cacggccaga aggagatggg ccgaggcgac ttccggaagg tgcccaatgg
      721 cctgcccggc gtcgagaacc gggtggacct catgcatgac ggcggggtcg tcggcggcca
      781 cctgaccccg aaccgctggg tccagctcat ctcgaccgcc ccggcccgcc tgttcggcct
      841 gccgcagaag ggcgcggtcg cggtcggcgc cgacgcggac ctggtcgtct acgacccgaa
      901 ccgacggcac accatcagcg cggcgagcca ccacatggac gtcgactact cgtgctacga
      961 ggggcgcgag gtgaccggag gcagcgacgt cgtcctatcc cggggcaagc tcatcgtgga
     1021 cggcgagacg tggctgggcg ccaagggcga cggccgcttc ctcaagcggg tccggagcga
     1081 gtatctgcgc tgacgggtac cctccccgga ccgatgcgcc tgcgtttcgt cctggtcgcc
     1141 gctctagcgg cgctggtcct tacggcctgt cagccgtccg cgtcaccatc ggccgagccc
     1201 tcggttgacg cctctgccgg ctccggcgat ccgctgccct cgatggtcgg ggatccggag
     1261 ctcgaggcga ccctgccgga cgaggcaggg gacatcaggt tctatcagac gttctcgatg
     1321 agcggcccgg acttcctgtc ggccgacatc gacgatcagt tcgtcaggtt cgtcgagtcg
     1381 ctgggcgcgg acatcgatga cgtgtcggtc gccttcgcgc tggggagcaa cttcgacgtg
     1441 accgataccg ccagcgtgtt cgcgttccag gtggacggcg cggaggcggc cgaccttgtc
     1501 gacga
//
LOCUS       DATBSW010000174        12262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_85904, whole genome shotgun sequence.
ACCESSION   DATBSW010000174 DATBSW010000000
VERSION     DATBSW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12262
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85904"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VIF63_04825"
     CDS             complement(<1..258)
                     /locus_tag="VIF63_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238739.1"
                     /translation="MTFVETVLLAAIAGGTILLGLPVGRLRNLSTRTQALLTTGAAGV
                     ILFLIWDILAAAVEPVEAAIEELAEGGPLSAVVVNGSAFAIS"
     gene            complement(423..1223)
                     /locus_tag="VIF63_04830"
     CDS             complement(423..1223)
                     /locus_tag="VIF63_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238740.1"
                     /translation="MIKKRQLLALLGSSALLLGMLVATTSLTSAGVEPDCNAVLCWTG
                     NGVSDGELDTVDCDENNTPYIYWVFASSKGVTSATLHFGGSGSGDYPMNTDNGTWKLQ
                     NGPWFDLETLTAWVTYTGTPENNPHLTISHGCPGAEETEAPSFEASQEGETEAPSEAP
                     SEAPSFEASQEGETEAPSDAPSDAPSFEASQEGETDAPSFEASQEGQTDAPSFESSQE
                     GATEPSTDSLGTNGTSGPADGAWLLVVALGVLLGSVVVLTPARAKSRR"
     gene            complement(1578..2321)
                     /locus_tag="VIF63_04835"
     CDS             complement(1578..2321)
                     /locus_tag="VIF63_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238741.1"
                     /translation="MLAGAFLAVGLAGVAAAHNKSYSLTCDHGLKVTLTNYNTSNDNS
                     LKIYLDGNLVPVVDIADFGGSESYESGPLDPFLSHTAHVVVVAHDDPTGSIGWSFDVT
                     LEIGACQEPSAPPTLEPTLEPTLEPTLEPTLEPTLEPTLEPSTPPSEEPTLEPSTPPS
                     TPPSAPPSEEPSAPPSFFASQEGETDAPSDAPSFAQTQQAATEPSTDSFDASGTSRPA
                     NDAWLLVVALGVFLGTIVVLTPARARDRR"
     gene            2645..4387
                     /gene="argS"
                     /locus_tag="VIF63_04840"
     CDS             2645..4387
                     /gene="argS"
                     /locus_tag="VIF63_04840"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY8238742.1"
                     /translation="MIPTASAIRGQPGEHTLRGLVRSALVRAWAAAAEAGRLPPAGPE
                     VEAEIDVQRPAKPEHGDYMTNVALRLARPLRMPPAAIATALVEAVVAEARSAPGAPIA
                     DASMAGPGFVNLRIADAALAKVIDSARDGDTTWGRVPVAKGTDVRHVNVEFVSANPTG
                     PLHVGNARGAFVGDLLARVLEAGGQRVTREYYFNDSGAQVRNLGATILAIREGGDIPE
                     EGYHGEYVVDLAPHLPDELVVAPTGLVADQPSAREQWEFRVGQWASGRIRAGIEASLA
                     NLGVKFDVWTTEASLHDDGWVDRAMEKLRAGGHLYEQDGALWFRSTAFGDDKDRVLVR
                     SNGSRTYFAADIGYVLEKFSRGYDHLIYIWGADHHGTVARLRNAAEAMGLDKSAVEVI
                     LTGWVHFTENGQELSMSKRAGTFITLDDLLAELGVDAARWYFASRGANVNMDIDIEVA
                     KRQSSENPVYYVQYAHARIASILRKAAGTGLAAAPSVASSLSDGPEGTLARIVARLPE
                     VVEDAATAQETQAVTTYATELATAFHAFYRDARVVDPDAPERSAKRLALVDATRISLA
                     NALALLGISAPESM"
     gene            complement(4401..5663)
                     /locus_tag="VIF63_04845"
     CDS             complement(4401..5663)
                     /locus_tag="VIF63_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238743.1"
                     /translation="MHRPQAALLAAILALAACGTDGPTAAPTPAATTGPTPPSSLPAP
                     TEAASARPAAEVFAEIRAAVESIRGLQPTSEVDPVTIDEAQLVKNFTAEFDAGQTAEQ
                     LKNSEDLLITLGLLLPGSSLRALTLDLQAGQVVGYYSPDKDELFVVSRSGGMGPLEEA
                     TYAHEFTHQLQDQAFKLDALGLDDIDQSDRQLGVLGLVEGDASYVQSQWMFANLDSQE
                     MGEILAAALDPKALEALQNAPAYLRDTATFPYQDGLAFVTRLVTTGGFDAVNAAFADP
                     PASTEQVLHPEKYVDREPPIEVTLPNIAGSLGSGWSEAGQDTLGELLLRTWLREGKVA
                     STAARAAAVGWGGDRLALLRGPGGVMSVGMVTEWDSVADADEFATAATTATGSLSPGA
                     RVLHAAGSTRVLVALGAQADAVIAALAD"
     gene            6176..6862
                     /locus_tag="VIF63_04850"
     CDS             6176..6862
                     /locus_tag="VIF63_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8238744.1"
                     /translation="MEITWYGQTCVRLRGRDAVVVADAYQSIVGPTGRGITADIATFS
                     HPDDAPLAKAKGKRTRDGKTVLPTSLESTFSLDGPGEYEVKNVLLTGVRTYRDDTGSE
                     SKRRGTAFVVELDGLHTIHLGDVGHLLTQEELGDIGSVDIACVPVGGALNAAKTAELV
                     AQLDPKLVIAMPVCLDDADCADALAKFFHEMGGDPTAQPKLSVSISTLPQETTTVLLE
                     SRGKVAAASG"
     gene            complement(6859..8232)
                     /gene="hutI"
                     /locus_tag="VIF63_04855"
     CDS             complement(6859..8232)
                     /gene="hutI"
                     /locus_tag="VIF63_04855"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY8238745.1"
                     /translation="MGLRLIQGGRAADGQPGLLIERAAEVATMAGGIRFGVRMDDPSL
                     LVAPGGDANHEAAPAVATWEGRILATGPRNDLLQVLEGSGYPIARFARLDADGGTITP
                     GLIDPHTHLLFGGSREGELVMRQRGADYLAILQAGGGILSTVAATRAASDDDLADHGR
                     RWLAEMLAHGTTTIEAKSGYGLDLPTELRLLEIAYKLGKEGPVDVLPTWLGAHAVAPE
                     FRARPDGTEAYVRHLLEEQLPGVVAQGRARFADVFCERGVFSPEQSRRVLEAAARLGL
                     GSRLHADELAPSGGAELAADIGALSADHLAVPSAAGIDALAAAAAEGHPVVAVVLPAT
                     TWFLMHDERAPARTFIDRGIPVALATDFNPGTSPTPSLPLVMTVACLDLKLTPSEALA
                     ATTINAAAALGLADEVGSIETGRHADLAIWNVPTHRQIPYWPAADLVRAVVKRGRVVL
                     ERAASAG"
     gene            complement(8256..9221)
                     /gene="ftcD"
                     /locus_tag="VIF63_04860"
     CDS             complement(8256..9221)
                     /gene="ftcD"
                     /locus_tag="VIF63_04860"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY8238746.1"
                     /translation="MLVESVPNYSEGRRIDVVDRLADAVATTDGAYLLDRTSDPSHNR
                     SVLTLAGDAAAVERALEATIGVAIDTIDMEQHTGEHPRIGAVDVIPFVPLGSTTLEAC
                     VVLARGFGLRIAERFGLPVYLYARAATRPDRVRLADVRRGGYEGVRDEIGAAAGDRAP
                     DFGPHRTHPRAGAVAVGARPFLIAWNINLDTGDVEVAKRIARSVRESGGGLRAVQGNG
                     FMIEELGAAQVSMNLLDFETTPMWQVYDEVDRLASEEGVGIRESELIGLAPQAAFDGV
                     AAHAGVTDRDAEARFAAAVSYLRLREPDLSMVLEQRLEAVRSATG"
     gene            9405..9590
                     /locus_tag="VIF63_04865"
     CDS             9405..9590
                     /locus_tag="VIF63_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238747.1"
                     /translation="MIASQLILDQRIAELHEIGTREYADQAASGASASTVARITAAIR
                     SLFGSAPTSRPVRVAAH"
     gene            9652..10980
                     /locus_tag="VIF63_04870"
     CDS             9652..10980
                     /locus_tag="VIF63_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238748.1"
                     /translation="MAIAKNPFAVRDFRLLWIGEAVSSVGDQFALIALPWLALVLTGS
                     PLALGGVMAVMAIPRALLMLLGGVSVDRFSPRRVMLASNAIRLVAVAILGGMVLTGGV
                     QLWMLYAFALIFGVADAFFFPAQTSIVPELVEPDVLQRANGIVQGTAQGTVLVGPALA
                     GVVIAAFGGSASTATAAGIGVALLVDAGTFLVSLVSLWLIRPRTHVAQNTGSIVDSIR
                     EGVRFVWASPSMRAMITVSLSANLLIVGPIEVGLPYIAYTRLPEGAAAFGIMIAAFGG
                     GSLIGLAFGAMLPAPRPSRLGAVVVVPMAIAGLSLAALAAAHSTLVACLLTGVAGVAL
                     GYGNLLALTWIQGRIPPELMGRVMSVLITGSVGLVPVSSFIAGAAVEVSLEATMLIGG
                     GAMALLALGSLLSRPVRNLGLEQLQGAAPTGDSVDAPVGGTETGPAEAGA"
     gene            complement(10994..>12262)
                     /gene="hutU"
                     /locus_tag="VIF63_04875"
     CDS             complement(10994..>12262)
                     /gene="hutU"
                     /locus_tag="VIF63_04875"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8238749.1"
                     /translation="SWIYIGTQGILQGTYETFAELARQHFGGSLRGRVVLTAGLGGMG
                     GAQPLAVTMNEGVCLAIEVDPVRANRRHDIGYVDRVTDDPAQALSMARVAAEKGQAIS
                     IALVGNAAEIEPAWAKAGERFDVVTDQTSAHDSLVGYVPAEIDLADAALLREHQPDEY
                     VRRATASMADHVRAMLAFQRAGSIVFDYGNNLRAQAKDAGVPDAFDYPGFVPAFIRPQ
                     FCEGRGPFRWVALSGDPADILRTDRAILQLFPEDESLARWIKLAEERVPFQGLPARIC
                     WLGYGERAKAGLAFNELVRTGEVSAPIVIGRDHLDSGSVASPNRETESMRDGSDAVAD
                     WPLLNALVNTAAGATWVSIHHGGGVGIGYSQHAGMVVVADGTELAAQKLERVLTTDPA
                     MGVLRHADAGYERAIEVARERDVRIPMLDE"
BASE COUNT         1745 a         4300 c         4303 g         1914 t
ORIGIN      
        1 cgagatcgcg aacgcagaac cgttgacgac aacggccgag agcggcccgc cctccgccag
       61 ctcctcgatc gccgcttcga cgggctcgac ggccgcggcg aggatgtccc agatcaggaa
      121 gaggatgacg cccgctgcac ctgtcgtgag gagtgcctgc gtccgcgtcg agagattcct
      181 gagacgaccg accgggaggc cgagaaggat cgtgccgcct gcaatcgccg ccaggagcac
      241 ggtctcaacg aacgtcatcg cgccggttcg acctcccagc atctccagca caagtcaagg
      301 tcgcttgcac gcgatgcgca gggtaccttg aatcagaaag accgctcgcc gggcatgaaa
      361 aaacgagggc ccggttgccc gggccctcgt tccttaggtc aggtccttgt cgggactgct
      421 ccctagcgcc ggctcttggc ccgcgccggg gtcagcacga cgacgctccc gaggagcacg
      481 ccgagcgcga ccacgagcag ccatgcgcca tcagcaggac cggaggtccc gttggtgccg
      541 agcgagtccg ttgacggctc ggttgcgcct tcctggctcg actcgaagga cggagcgtcc
      601 gtctggcctt cctggctggc ttcgaacgac ggagcgtcgg tctcgccttc ctggctcgcc
      661 tcgaaggagg gagcgtccga gggggcgtca gagggggctt ccgtctcgcc ttcctggctc
      721 gcctcgaagg acggggcctc ggacggggct tcggacgggg cttccgtctc gccttcctgg
      781 ctcgcctcga aggacggggc ctcggtctct tccgcacccg ggcagccgtg gctgatggtg
      841 aggtgcgggt tgttctcggg agtccccgtg tacgtgaccc acgccgtcag cgtctcgagg
      901 tcgaaccacg gaccgttctg gagcttccag gtgccgttgt ccgtgttcat cgggtagtca
      961 ccggagccgc tgccaccgaa atggagcgtc gccgaggtga cgcccttcga gctggcgaac
     1021 acccagtaga tgtacggagt gttgttctcg tcgcaatcca cggtgtcgag ctcgccgtcg
     1081 ctgacgccat tgccggtcca acagaggacc gcgttgcagt cgggctctac gccggccgac
     1141 gtcaggctcg tcgtcgcgac gagcatgccg agcagcaatg cgctggatcc cagcagggcc
     1201 agcagttgcc ttttcttgat catgttctcg tttgtttcct ccgctatccc agacacggac
     1261 cggaccgaca agtgggctcc cgatggccca acgtggagtc aaacgggagc gtagtgtgca
     1321 tgagggccta tccgtcgtct agtacttccg gacccccgca aactgcacct aacgatccca
     1381 gaccgcacca atctgccatc gattttggtg cgtttggtcg atcatcgaga caagaaccgg
     1441 gacagcagcc gtgtcgcgca tatgactttc gtactgtggg tctggtgcag gcccactccc
     1501 agcgggtggc atggaacgag ggcccggttg cccgagccct cgttgccagc gttcagcttc
     1561 agcgcggggg tacggcgcta acgccggtcc ctggctcgcg ccggtgtcag caccacgatg
     1621 gtcccgagga acaccccgag tgcgaccacc agcagccagg cgtcattcgc cggacgggag
     1681 gtcccactcg cgtcgaacga gtcggtcgac ggctcggtcg cggcctgctg ggtctgcgca
     1741 aacgaagggg catcggacgg agcatccgtt tcgccttcct ggctcgcgaa gaacgacggc
     1801 ggggcgctcg gctcctcgct cggcggggcg ctcggcgggg tgctcggcgg ggtgctcggc
     1861 tcgagggtcg gctcctcgct cggcggggtg ctcggctcga gggttggctc caaggtcggc
     1921 tccagcgtcg gctcgagggt tggctccagc gtcggctcga gggtgggctc gagcgtcggc
     1981 ggggcgctcg gctcctggca tgcgccgatc tcgagcgtga cgtcgaagga ccagccgatg
     2041 ctcccggtcg ggtcgtcatg ggcgacgacg acgacgtggg cggtgtggct caggaacggg
     2101 tcgagcggtc cgctctcata ggactcgctc ccgccgaagt cggcgatgtc cacgacgggc
     2161 acaaggttgc cgtcgaggta gatcttgagc gagttgtcat tcgaggtgtt gtagttggtg
     2221 agcgtgacct tcagtccgtg atcgcacgtc agcgaatagg acttgttgtg ggcggcggcg
     2281 acgccggcga gccccactgc caggaatgct ccggcgagca tcgcggcgag ccttctgcga
     2341 gttttcatga tcgatctttc cccccggtcg cacgacttgc gctgatgact gcgtccgggt
     2401 cgagaagtcg ccgcttggct ccatcccccc ggatgctgcc tttgcaatct atcgggtggt
     2461 cccataggcg tcgaggacct tcctcctaca ccggaccaca ctccgccgcc cgagaccgca
     2521 cccaagtcca actcgcacgc ttcgtttctg tcgactcccg gcaacggggt cccacccctt
     2581 tggtcctacc cgccgcgacc ggcacgggcg cgatgccgca ccgtgacccc gtaccatccc
     2641 gaacgtgatc ccaaccgctt ccgcgatccg cggccagccg ggcgagcaca cgctccgcgg
     2701 gctcgtccgc agtgcgctgg tgcgcgcgtg ggcggctgcc gcggaggcgg gccggctgcc
     2761 gccggccggg ccggaggtag aggccgagat cgacgtgcag cggcccgcga agcctgagca
     2821 cggcgactac atgaccaacg tggcgcttcg cctggcacga ccgttgcgga tgccgccggc
     2881 cgcgatcgcg accgcgctgg tcgaggcagt ggtcgccgag gcacggtcag cccctggcgc
     2941 tccgatcgcc gatgcgtcca tggcaggccc gggcttcgtc aatctgcgga tcgccgacgc
     3001 ggcgctcgcg aaggtcatcg attccgcgcg agacggcgac acgacctggg gccgcgtgcc
     3061 ggtcgccaag gggaccgacg tccgtcacgt caacgtcgag ttcgtctccg ccaatccgac
     3121 cggcccgcta cacgtcggca acgcgcgcgg tgcgttcgtc ggcgacctgc ttgcgcgcgt
     3181 gctcgaggcc ggaggtcagc gcgtcacgcg tgagtactac ttcaacgact ccggcgcgca
     3241 ggtccggaac ctcggcgcaa cgatcctcgc catccgcgag ggcggcgaca ttcccgagga
     3301 gggctatcac ggcgagtacg tcgtcgacct ggcgccgcat ttgcccgacg agctggtggt
     3361 tgcgccgacc ggcctcgtcg ccgaccagcc gagcgcccgg gaacagtggg aattccgggt
     3421 tggccagtgg gcgtccggtc gcatccgggc gggcatcgag gcgtcgctcg ccaacctcgg
     3481 cgtcaagttc gacgtctgga cgaccgaggc gagcctgcac gacgacggct gggtcgaccg
     3541 ggccatggag aagctccgcg cgggcgggca cctgtacgag caggacggtg cgctctggtt
     3601 ccgctcgacc gccttcggtg acgacaagga ccgcgtcctg gtccggtcga acggatcgcg
     3661 gacctacttc gcggccgaca tcgggtacgt gctcgagaag ttcagccgcg gctacgacca
     3721 cctgatctac atctggggtg cggaccacca tgggacggtt gcccggctgc gcaacgcggc
     3781 cgaggcgatg ggcctcgaca agtcggccgt cgaggtgatc ctcaccggct gggtgcactt
     3841 caccgagaac ggccaggagc tgtcgatgtc caagcgggcc ggcacgttca tcaccctcga
     3901 cgacctcctt gccgagcttg gcgtcgatgc cgcgcgctgg tacttcgcct cacggggcgc
     3961 gaacgtgaac atggacatcg acatcgaggt cgcgaagcgc cagtcgagcg agaacccggt
     4021 ctactacgtg cagtacgcac acgcgcgcat cgcgtcgatc ctgcgcaagg ccgcgggcac
     4081 ggggttggct gcggcgccgt cggttgccag ctcgctgtcc gacgggccgg agggcacgct
     4141 ggcgaggatc gtcgcgcgcc ttcccgaggt ggtcgaggac gcggccacgg cccaggagac
     4201 gcaggcggtc accacgtacg ccaccgagct cgcgacggcg ttccatgcgt tctatcggga
     4261 cgcccgcgtc gtggatccgg atgcgcccga gcgctcggcg aagcgcctcg ccctggtgga
     4321 tgcgacccga atctcgctcg cgaacgcgct cgccctgctg gggatctccg cgcccgagtc
     4381 gatgtagcgg acggcgacac ctagtcggcg agcgcggcga tgacggcgtc ggcctgcgcg
     4441 ccaagcgcga ccagcacccg cgtcgagccc gcggcgtgca agacgcgcgc tcccggtgac
     4501 aggctgccgg tcgcagtcgt ggcggccgtc gcgaactcgt ccgcgtcggc gacgctgtcc
     4561 cactcggtca ccatgcccac gctcatcacg ccgccgggac cgcgcaggag ggcgaggcgg
     4621 tcgccgcccc agccgacggc cgcggcgcgc gcggcggtgg acgccacctt gccctcgcgc
     4681 agccaggttc gaaggagcag ctcgccgagc gtgtcctggc ccgcttcgga ccagcccgat
     4741 ccgagcgatc ccgcgatgtt cgggagcgtc acctcgatgg gcggctctcg atcgacgtac
     4801 ttctccgggt ggagcacctg ctcggtcgac gccggcggat cggcgaatgc ggcattgacc
     4861 gcatcgaagc cgccggtcgt cacgagccgg gtcacgaacg cgagcccgtc ctggtatggg
     4921 aatgtcgccg tgtcgcgcag gtacgccggt gcgttctgga gcgcctcgag cgccttcggg
     4981 tcgagcgcgg cagcgaggat ctcgcccatc tcctgcgagt cgaggttcgc gaacatccac
     5041 tggctctgga cgtagctcgc gtcgccttcg accaggccga gcacgcccag ctggcgatcg
     5101 ctctggtcga tgtcgtccag gccgagggca tcgagcttga aggcctggtc ctggagctgg
     5161 tgcgtgaact cgtgggcgta cgtcgcctcc tcgagcggac ccatgccgcc cgatcgcgag
     5221 acgacgaaca gctcgtcctt gtcgggcgag tagtagccca cgacctggcc ggcctggagg
     5281 tcgagcgtca gggcgcggag cgacgagccg ggcagcaaca gcccgagcgt gatcagcagg
     5341 tcctcggaat tcttgagctg ctcggcggtc tgcccggcgt cgaactcggc ggtgaagttc
     5401 ttgacgagct gggcctcgtc gatggtcacc gggtcgactt ccgacgtcgg ctgcagcccg
     5461 cggatcgact cgacggcggc gcggatctcg gcaaagacct cggcggcggg tcgagccgat
     5521 gccgcctcgg tcggggcggg caggctcgag ggcggcgtcg gaccggtcgt cgcggcgggg
     5581 gtcggggccg cggtggggcc gtcggtgccg cacgcggcaa gcgcgaggat ggcggcgagc
     5641 agcgccgctt gtggtcggtg catgaaggtg gcgatcctac ccagccacag gcgctccgtc
     5701 gcgggctgat ggtcctcgtt ggcgtttctt aatcggttcg ggcgacgggc ctgaacaccc
     5761 cacgcatagt ctcgattgcg gaccgaaccc tcgtggttcc atcctccctc cctccgaccg
     5821 gaggcgccga ttgggcaggc gcctccggtc ttttctttgc ctcgcgatgg gctcatgggc
     5881 tcatgggctc gccgccgctt cccgcccgct gccgcgtccc gcccgctgcc ccgtcatatg
     5941 cgcggaatcg gcggccacca ttcgatcgct gttggcaatg cctgttgcca gcatatgaat
     6001 ggctcgccgg tcggtccttc gcatgctggc gacacgcatc gccaagggaa ggtgcatgcc
     6061 ttgccccgac ttcacgcata ggagtaggtc cgttcgcgga cggcgggtag gcggcccgcg
     6121 acacacggaa cggcaggcaa tcggtgggtg ggcggcgtgt gccagaatca cggggatgga
     6181 gatcacctgg tacgggcaga cgtgtgtgcg gttgcggggg cgggatgccg tcgtggtggc
     6241 tgacgcgtat cagtcgatcg ttgggccgac cgggcgcggc attaccgcag atatcgcgac
     6301 gttcagccat cccgacgacg cgccgctggc caaggccaag ggcaagcgca cgcgcgatgg
     6361 caagacggtg ctgccgacca gcctggagtc cacgttcagc ctcgacggcc ccggcgagta
     6421 cgaggtcaag aacgtcctcc tgaccggcgt acgcacgtac cgcgacgaca ccgggagcga
     6481 gagcaaacgg cgcggcaccg ctttcgtcgt cgagctcgac gggctgcaca ccatccacct
     6541 cggcgacgtt ggtcacctgc tgacccagga ggagctcggc gacatcggct cggtcgacat
     6601 tgcctgcgtg ccggtcggtg gcgcgctcaa cgcggcgaag accgccgagc tcgtcgccca
     6661 gctcgacccc aagctcgtca tcgcgatgcc cgtctgcctc gacgacgcgg actgcgccga
     6721 cgcactcgcc aagttcttcc acgagatggg cggcgacccg accgcgcagc cgaagctctc
     6781 ggtttcgatc tcgaccctgc cccaggagac caccaccgtg ctcctggaat cgcgcggaaa
     6841 ggtcgcggcg gcgagcggtt agcccgcgct cgcggctcgc tcgaggacga cccggccgcg
     6901 cttcaccacg gcccgaacga ggtcggcggc cggccagtac gggatctggc ggtgggtcgg
     6961 cacgttccag atcgcaaggt cggcgtgacg gcccgtctcg atcgagccca cttcgtccgc
     7021 gaggcccagc gccgcggcgg cgttgatcgt cgtcgcagcg agcgcctccg agggcgtgag
     7081 cttgaggtcg aggcacgcga cggtcatcac gaggggcagg ctgggcgtcg gcgacgtgcc
     7141 cgggttgaag tcggtggcga gggcgaccgg gatgccgcgg tcgatgaacg ttcgcgcggg
     7201 cgcgcgctcg tcgtgcatga ggaaccatgt cgtggccgga aggacgacgg ccacgacggg
     7261 gtggccctcg gccgcggcgg cggcgagcgc gtcgatcccc gccgctgatg ggaccgcgag
     7321 gtgatcggcg gagagcgcgc cgatgtccgc ggcgagctcc gcgccgccgc tcggtgccag
     7381 ctcgtcggcg tgcagccgcg agccgagccc gagccgcgcc gcagcctcga gcacacgccg
     7441 tgactgctcc gggctgaaca cgccgcgctc gcagaagacg tccgcgaacc gcgcccggcc
     7501 ctgcgcaacg acgccgggca gctgctcctc gagcaggtgg cggacatacg cctccgttcc
     7561 gtccggtcgg gcgcggaact ccggggccac cgcgtgcgcc ccgagccacg tcggcaggac
     7621 gtcgaccggg ccttccttgc cgagcttgta ggcgatctcg agcaggcgca gctccgtcgg
     7681 caggtccagc ccgtagcccg acttggcctc gatggtcgtc gtgccgtggg cgagcatctc
     7741 ggccagccag cgccggccgt ggtcggcgag gtcgtcgtcg ctcgcggcgc gcgtcgcggc
     7801 gaccgtggac aggatcccgc cgccggcctg gaggatcgcg aggtagtcgg cgccgcgctg
     7861 gcgcatcacc agctcgcctt cgcgcgagcc gccgaacagc aggtgcgtgt gcgggtcgat
     7921 caggcccggc gtgatcgtcc cgccgtcggc atcgagccgc gcgaaccggg cgatcgggta
     7981 gccgctgccc tcgaggacct ggaggaggtc gttgcgcggg ccggtggcga ggatccggcc
     8041 ttcccacgtc gcaaccgcgg gcgcggcttc gtgattggcg tcgccacccg gcgcgacgag
     8101 cagcgaggga tcgtccatcc gcacgccgaa gcggatgccg ccggccatcg tggcgacctc
     8161 ggccgcgcgc tcgatcagca ggcccggctg cccgtcagcc gcgcgcccac cctggatcag
     8221 tcggaggccc atcggcgtcg tcgtgcctgg ctcgcctagc ctgtggccga gcgaaccgcc
     8281 tcgagacgct gctcgaggac catgctgaga tcgggctcgc gcaggcggag gtacgacacg
     8341 gcagcggcga accgcgcctc cgcgtcccga tcggtcacgc cggcgtgcgc cgccacgcca
     8401 tcgaacgccg cctgcggcgc gagcccgatc agctcggatt cccgaatgcc gacaccctcc
     8461 tccgacgcga gccggtcgac ctcgtcgtag acctgccaca tgggcgtggt ctcgaagtcg
     8521 aggaggttca tcgacacctg cgccgcgccg agctcctcga tcatgaagcc gttgccctgg
     8581 acggcgcgca ggccgccgcc cgattcgcgg acgctgcgcg cgatccgttt cgcgacctcg
     8641 acgtcgcccg tgtcgaggtt gatgttccag gcgatgagga acggccgggc gccgaccgcg
     8701 acggcgcccg cgcgcgggtg cgttcgatgc gggccgaagt cgggagctcg atcgcccgcc
     8761 gccgccccga tctcgtcgcg cacgccctcg tagccgccgc gccgcacgtc ggcgaggcgc
     8821 acccgatcgg ggcgcgtcgc cgcgcgcgcg tacaggtaca ccgggagccc gaaccgctcc
     8881 gcgatccgca gcccgaagcc gcgcgcaagg acgacgcacg cctcgagggt cgtggacccg
     8941 agcgggacga acgggatgac gtcgacggcg ccgatgcggg ggtgctcgcc cgtgtgctgc
     9001 tccatgtcga tcgtgtcgat ggcgaccccg atcgtcgcct cgagcgcgcg ctcgaccgcc
     9061 gccgcatcac ccgcgagggt cagcacggag cggttatggg agggatcgct cgtccggtcg
     9121 aggaggtagg cgccatccgt cgtcgccacg gcgtccgcca accggtcgac gacgtcgatt
     9181 cggcgccctt cggagtagtt cgggacggac tcgaccagca tcccgagatc gtgacacgcg
     9241 gcgcgtccac ccgtcggacc tggtcagttg tgctcgaaag gaggcgaccc ggtgccttct
     9301 cgatcgttcg atgatctttt tcgcaaatca gtattgacat ttcgaaagtc tgtgtgccat
     9361 agtgcccaca tcaagcgcac agcctcgtaa gaagggaacc cgaaatgatc gcctcgcagc
     9421 tcatcctcga ccagcgcatc gccgagctcc acgagatcgg cacccgggag tacgccgacc
     9481 aggccgcgag cggcgcgtcc gcctcgaccg tcgctcggat cacggcagcg atccgctcgc
     9541 tgttcggcag cgcgcccacc agccgtcccg tccgggtcgc agcacactga gtcgcgaccc
     9601 acgacggccc acctgccaac cgccgaccca ccaaccacag cggtgaccga catggcaatc
     9661 gcgaagaacc cgttcgccgt ccgcgacttc cgcctcctgt ggatcggcga ggcagtctcg
     9721 agcgttggcg atcagttcgc cctcatcgcc ctgccctggc tcgccctcgt cctgaccggc
     9781 agcccgctcg cgcttggcgg cgtgatggcc gtgatggcca tcccgcgggc cctgctgatg
     9841 ctcctcggcg gcgtctccgt cgatcggttc tcgccgcggc gggtgatgct cgcctcgaac
     9901 gcgatccgcc tcgttgccgt cgcgatcctc ggcggaatgg tgctgacggg tggggtccaa
     9961 ctctggatgc tctacgcctt cgcgctcatc ttcggggtcg cggacgcgtt cttcttcccc
    10021 gcccagacat cgatcgtgcc cgagctcgtc gagcccgacg tgctccagcg cgccaacggc
    10081 atcgtccagg gcaccgccca ggggacggtc ctcgtcgggc ccgcgctcgc cggcgtcgtg
    10141 atcgcggcat tcgggggcag tgcatccacg gcgacggccg cgggcatcgg cgtggcgctg
    10201 ctcgtcgatg cggggacgtt cctcgtctcc ctcgtcagcc tctggctcat ccggccgagg
    10261 acgcacgtcg cccagaacac cggctcgatc gtcgattcga tccgggaggg cgtccgcttc
    10321 gtctgggcgt ccccctcgat gcgcgcgatg atcaccgtga gcctgtcggc caacctgctc
    10381 atcgtcgggc cgatcgaggt cgggctgccg tacatcgcct acacccggct gcccgagggc
    10441 gcggcggcgt tcggcatcat gatcgccgcg ttcggcggtg ggtcactgat cggtctcgcc
    10501 ttcggggcga tgctgcccgc accgcgcccg tcgcgcctgg gcgcggtcgt cgtcgtgccc
    10561 atggcgatcg ccgggctgag cctcgccgcg ctcgccgccg cccactcgac cctcgtcgcg
    10621 tgcctcctga ccggcgtcgc cggcgtggcg ctcggctacg gcaacctgct cgcgctgacg
    10681 tggatccagg gccgcatccc acccgagctc atgggccggg tcatgagcgt gctcatcacg
    10741 ggctcggtcg ggctcgtgcc ggtctcgtca ttcatcgcgg gcgctgcggt cgaggtcagc
    10801 ctcgaggcga cgatgctcat cggcggcggc gcgatggcgc tcctcgcgct gggttcgttg
    10861 ctgtcccggc cggttcgcaa cctggggctc gagcagctcc agggggccgc gccgacgggc
    10921 gactcggtcg acgcgcccgt tggcgggacc gagaccggac ccgccgaagc aggcgcctag
    10981 acgctcgccc gcctcactcg tcgagcatcg ggatgcgcac gtcgcgctcg cgggcgacct
    11041 cgatcgcccg ctcgtagccc gcgtcggcgt ggcgcaggac gcccatcgcg ggatcggtgg
    11101 tgaggactcg ctccagcttc tgcgctgcga gctccgtgcc gtcggcgacg acgaccatgc
    11161 cggcatgctg cgagtagccg atcccgacgc cgccgccgtg gtggatcgag acccaggtcg
    11221 cgccggcggc cgtgttcacc agggcgttga ggagcggcca gtcggccacg gcgtcggagc
    11281 cgtcgcgcat cgactcggtc tcgcggttcg gtgacgcgac ggagccggag tcgaggtggt
    11341 cccgcccgat cacgatcggc gccgagacct cgccggtccg gaccagctcg ttgaaggcga
    11401 gcccggcctt cgcccgctcg ccgtagccga gccagcagat gcgcgccggc agtccctgga
    11461 acggcacccg ctcctcggcc agcttgatcc accgagcgag cgactcgtcc tcggggaaca
    11521 gctggaggat cgcgcggtcc gttctgagga tgtccgcggg gtcgcccgac agcgcgaccc
    11581 agcggaacgg cccgcggccc tcgcagaact ggggccggat gaatgcgggc acgaagcccg
    11641 gatagtcgaa ggcatcgggc acgccggcgt ccttcgcctg cgcgcgcagg ttgttgccat
    11701 agtcgaacac gatcgagccg gcccgttgga aggcgagcat cgcccggacg tggtctgcca
    11761 tcgacgcggt cgcgcgacgg acgtactcgt cgggctggtg ctcgcgcagg agcgcggcgt
    11821 cggcgaggtc gatctcggcc gggacgtagc cgaccagcga gtcgtgggcc gatgtctggt
    11881 cggtcacgac gtcgaaccgc tcccccgcct tcgcccaggc gggctcgatc tcggcggcat
    11941 tgccgacgag cgcgatcgag atcgcctggc ccttctccgc cgcgacacgc gccatcgaca
    12001 gcgcctgagc ggggtcgtcg gtcacccggt cgacgtagcc gatgtcgtgg cgccggttcg
    12061 cccgcaccgg atcgacctcg atcgcgaggc agaccccttc gttcatggtt acggcgagcg
    12121 gctgggcgcc gcccatcccg ccgagcccgg cagtgaggac gactcgaccg cggaggctgc
    12181 cgccgaagtg ctggcgggcg agctccgcga acgtctcgta cgtgccctgg aggatgccct
    12241 gggtcccgat gtagatccac ga
//
LOCUS       DATBSW010000175         7663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_968334, whole genome shotgun sequence.
ACCESSION   DATBSW010000175 DATBSW010000000
VERSION     DATBSW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968334"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VIF63_04880"
     CDS             <1..542
                     /locus_tag="VIF63_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8238750.1"
                     /translation="YKVRPASVRFETGTGNFEGYAGALAAVEYLADIGRTYGGAAAGS
                     SRREAVLSGMHAIRAYELELYRHLAARLAAIDGIEIVGLTAGEDMERRTPTAAIKVAG
                     VTPHAAAEHLGQRGIAVWDGDFYATGLIERLGLAPDGVVRIGLTHYNTSAEVDRLADE
                     LAALVESARRSAVEPAAAS"
     gene            569..1438
                     /locus_tag="VIF63_04885"
     CDS             569..1438
                     /locus_tag="VIF63_04885"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY8238751.1"
                     /translation="MAVETVRAFGGQSMTAPLREALLKAPGPAFGRAYDDPAHGFLHA
                     CDLEVAKRQHAAFAELLGSLGPTVHILDAEIDSPDLLYTFDPLLVTDRGAIPLRPGKP
                     NRQPEPAVIEAWTRDHGIPTAGRIEAPGTIEGGDTFWLRPDLFCIGRTLRTNQAGAEQ
                     LAALVGGDVRIFDVPYWKGPEELVHLMSVISPIADELAVVYMPLMPVGLWQLLQDLRY
                     RLIEVPDEEYPTLGCNVLAVRPGVVIAAEGNPRTAAAMAAHGVEVHTYEASEIGVNGS
                     GGPTCMTRPILRG"
     gene            1435..2703
                     /locus_tag="VIF63_04890"
     CDS             1435..2703
                     /locus_tag="VIF63_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEY8238752.1"
                     /translation="MTTLSELEQQAVEAVDAEWTAGVARQLVRVPSVTGDEGAVQDLV
                     ASRLADAGCVVERLEIDPADARRDPDWPGDEMPRDRLPIVLGRLGPTGGRRIVLVGHV
                     DVVPVGDPATWSHDPWAGEVVGDRLYGRGAVDMKGGVASILGAVAALTASGLAEGLGG
                     EILVASVPSEEDGGQGMLAAIRAGVTGDMAVITEPTGLDVVIAHAGAITFKLTVPGRA
                     AHASVRREGVSALDNLQTLVRALEADETARNVTETDPLMTAHGLPYATIIGKVSGGDW
                     ASTVIDRIEAEGRYGVKLGQTWRDAERDLQRAIDDANESDPFLRDHPATVELTGGKFS
                     SSRVPADHPLPVGLAGVVSALTGREPQMTGEPYGADMRLLVNEGNTPTVIFGPGDKRV
                     AHSADEWVSLAEVADCARVLAAWLVRELIA"
     gene            complement(2902..4170)
                     /locus_tag="VIF63_04895"
     CDS             complement(2902..4170)
                     /locus_tag="VIF63_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238753.1"
                     /translation="MSFLAHESAHLDEHGLERARRRTRWALFASVALGSTGHLAAVTV
                     AAIVGQDLLGTAALAGAPGAAVVFGAAVGAIALSSIMARRGRRLGLTTGYLLGIVGAL
                     IATEAVLIRSFPLLLLGTFIIGFGNSSNNLSRYAAADLVAPNRRAVAIGLVVWGSTVG
                     GIVGPWLAPIAGNLALGAGLPQYAGPFLVPVLFVGAAALLTFVFLRPDPFALAHETNI
                     RQASDAPALPAREIFARPAVLAALVALMAGQATMVLIMTMTPLHLTSHNHGLETVGLV
                     ISGHVAGMFALAPLSGWISQRIGNIRTIFLGTAVLAGASLLAGLAPPEHDEILFVALF
                     LLGWGWNLGFVAGSAMLSAGVELAARARVQGLADAAIWTTSAIASLGSGAVVAAAGFS
                     TLGFLGVALVLAPVWLLLTRRHVIAAGTAN"
     gene            complement(4202..5599)
                     /locus_tag="VIF63_04900"
     CDS             complement(4202..5599)
                     /locus_tag="VIF63_04900"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEY8238754.1"
                     /translation="MTTSPSFEPSTSTVDRARLQLLLERELATFAERHPRTRSLHDRA
                     RASLLDGVPMNWMVKWAGPFPLFLESASGAHATCADGHDYVDLCLGDTGAMAGHGPPA
                     LIAAVERQLRRGITTMLPTEDAAWVGEELTRRFGLPSWQFTLTATDANRFSLRLARAI
                     TDRPFVAVHEWCYHGSVDESFAVLDPADPASGRVVARRGNLGPAVDPAATTRVVPYND
                     LDALDRALADRQVAALLIEPALTNVGIVLPDPGYLEGVREITRRHGTILIHDETHTIC
                     AGPGGVTGAGHYEPDMLVIGKTIGGGIPVATYGMTTEVADRVRATIPIDDTDVGGIGG
                     TLAGYALSMAATRATLGEVLTAAAFERMLPLAERWERGVNEAIERRGAPWNVTRLGAR
                     AEYHFMPQPARDGAEQIQNSDPDLERLLHLWVMNRGVLMTPFHNMALMSPATTDADVD
                     RHTAAFDEALAALFD"
     gene            6322..7491
                     /locus_tag="VIF63_04905"
     CDS             6322..7491
                     /locus_tag="VIF63_04905"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEY8238755.1"
                     /translation="MTTLFSRRLRGVLALAFALATVPLAVPGALAAEGLTLTTPYPAL
                     TVSPGTDVTFNLAVETTAPARVDLSLSGVPASWTAEMHGGGFVVGAVQTDGDKSTDVR
                     LDVTVPEDATGTTRITVTASSTGSVVTLPLDIKVEVNAGGAVTVKPDYPAQRGPANQS
                     FTFNLSIDNGKEQDLSYNATGEGPAGWTVDVTLTGQAQAVNGTVKAGSTASVAVKITP
                     AANTTAQTYQNAVVVTVGDEQFPIELAVEVTGSYSLVLSTPTQLLSARGPSGSATTQT
                     FRITNTGTAPVTGVTVSVTPPTDWKVTFAPETTDSIAPGAFADIVATITPSGDAIAGD
                     YALTFTAKGKEASATDSAEVRFTVESSIIGGLIGVVLILAAIGGLWWVFRTYGRR"
BASE COUNT         1183 a         2632 c         2769 g         1079 t
ORIGIN      
        1 cgtacaaggt gcggccggcg tcggtgcggt tcgagaccgg taccggcaac ttcgagggct
       61 acgccggtgc gctggccgcg gtcgaatacc tcgccgatat cgggcgaacg tacggcggcg
      121 cggcagcggg gagctcccgc cgggaagcgg tcctctccgg catgcacgcg attcgcgcct
      181 acgagctgga gctctatcgc cacctcgcgg cgcgcctggc ggccatcgac ggcatcgaga
      241 tcgtcgggct caccgccggc gaggacatgg agcgacgcac cccgaccgca gcgatcaagg
      301 tcgccggcgt cacgccgcac gcggcggccg agcacctggg ccagcgcggc atcgccgtct
      361 gggacggcga cttctacgcg accgggctca tcgagcgcct cggcctggcg cccgacggcg
      421 tcgtgcgcat cggcctgacc cactacaaca ccagcgccga ggtcgatcgc ctcgccgacg
      481 agctggcggc gctcgtcgaa tcggctcggc ggtccgcagt ggagccggcc gccgcgagct
      541 gacgactggc ctcgatatgc tgcgcggcat ggcagtcgag acggtgcgtg cgttcggcgg
      601 gcaatcgatg accgcgccgc tgcgcgaagc gttgctcaag gcgccggggc ccgcgttcgg
      661 acgggcatat gacgatcccg cgcacggatt cctgcacgcc tgcgacctcg aggtcgccaa
      721 gcgccagcac gccgcattcg cggagcttct tgggtcgctc ggcccgaccg tccacatcct
      781 cgacgcggag atcgacagcc cggacctgct gtacacgttc gacccgctgc tcgtgaccga
      841 tcgcggtgcg atcccgctgc ggcccggcaa gcccaaccgg cagcccgagc cggcggtcat
      901 cgaggcctgg acgcgcgacc acggcatccc gacggccggt cggatcgagg cgcccgggac
      961 gatcgagggc ggcgacacgt tctggctccg cccggacctc ttctgcatcg gtcgcacgct
     1021 gcgcaccaac caggcaggcg cggagcagct cgcggcgctc gtcggtggcg acgtgcggat
     1081 cttcgacgtg ccctactgga agggtcccga ggagctcgtc cacctgatgt ccgtcatcag
     1141 cccgatcgcc gacgagctcg ccgtcgtcta catgccgctc atgccggtcg gcctgtggca
     1201 gctgctccag gacctgcgct accggctcat cgaggtgccc gacgaggaat acccgaccct
     1261 cggctgcaac gtcttggcgg tccggccggg tgtcgtgatc gcggccgagg gcaatccgag
     1321 gaccgccgcg gcgatggccg cccacggcgt cgaggtgcac acgtacgagg cgtccgagat
     1381 cggggtcaac ggctcgggcg gcccgacgtg catgacccgg ccgatcctgc gcgggtgacg
     1441 acgctgagcg agctcgagca acaggccgtc gaggcggtcg acgccgagtg gaccgccggc
     1501 gtcgcgcgcc agctggtccg ggttccgagc gtcacgggcg acgagggcgc ggtgcaggac
     1561 ctggtggcgt cacggctcgc cgacgcgggt tgcgtggtgg agcggctcga gatcgatccg
     1621 gccgacgcgc gccgcgatcc cgactggccc ggcgacgaga tgccgcgcga ccggctgccg
     1681 atcgtgctcg gccggctggg gccgacgggc gggcggcgaa tcgtgctcgt cgggcatgtc
     1741 gacgtcgtgc cggtggggga tccggcgacg tggagccacg acccatgggc cggcgaggtg
     1801 gtcggggacc gactgtacgg acgcggcgcg gtcgacatga aaggcggggt cgcgtcgatc
     1861 ctgggcgcgg tcgccgcgct caccgcgtcc gggctcgcgg agggcctcgg cggcgagatc
     1921 ctcgtcgcca gcgtgccctc ggaggaggac ggcggccagg gcatgctcgc cgcgatccgc
     1981 gccggcgtga ccggcgacat ggctgtcatc accgagccga cgggcctcga tgtcgtgatc
     2041 gcccacgccg gcgcgatcac gttcaagctc accgtgcccg gccgcgccgc gcatgcgtcg
     2101 gtgcgccgcg agggcgtgtc cgcgctcgac aacctgcaga cgctcgtccg cgcgctcgag
     2161 gccgacgaga ccgcccgcaa cgttaccgag accgacccgc tcatgaccgc ccacgggctg
     2221 ccctacgcga cgatcatcgg caaggtctcg gggggcgact gggcatcgac ggtgatcgac
     2281 cggatcgagg cggaggggcg ctacggcgtg aagctcggcc agacgtggcg cgacgccgag
     2341 cgcgacctcc agcgcgccat cgatgacgcc aacgagtcgg atccattcct gcgcgaccat
     2401 cccgcgaccg tggagctgac cggcggcaag ttctcgtcgt cccgggtccc ggccgatcat
     2461 ccattgccgg tcggcctcgc cggggtcgtc tcggcgctga ccggccgcga gccacagatg
     2521 accggcgagc cttacggggc cgacatgcgc ctgctcgtca acgagggcaa cacgccgacc
     2581 gtgatcttcg gaccgggcga caagcgggtc gcgcactcgg ccgacgagtg ggtctcgctg
     2641 gccgaggtcg ccgactgcgc ccgcgtgctc gccgcgtggc tggtccggga gctcatcgca
     2701 taggtcccgt ctaccgcgga tcggggcttg gcggaccgtc gtccgagcgg ggcgcgagat
     2761 ttctatgccc atcggtacat aacgagggcg cgggcgccag cggcgcgccc ttcgacgaac
     2821 gcgcttcgcc cgttatgtcg cgattggcat agttttggcg cgccggggag acgccacggc
     2881 gaagcggtcg ggccgcgacg attagttcgc tgtccccgcc gcaatcacgt gacgccgcgt
     2941 cagcagcagc cacacgggcg ccagcaccag cgccacgccc aggaagccga gcgtcgagaa
     3001 gcccgccgcg gcgaccaccg cgcccgagcc caggcttgcg atcgcagagg tcgtccagat
     3061 cgcggcgtcg gcgagcccct gaactcgtgc ccgggcagcg agctcgaccc ccgccgagag
     3121 catcgcgctg ccagccacga acccgaggtt ccagccccaa ccgagcagaa acagggccac
     3181 gaacaggatc tcgtcgtgct ccggcggcgc cagcccggcg agcagtgagg cgcccgcgag
     3241 caccgccgtg ccgaggaaga tcgttcggat gttgccgatc cgctgcgaga tccagcccga
     3301 caacggggcc agcgcgaaca tccccgcgac gtggccgctg atcacgaggc cgacggtttc
     3361 gaggccgtgg ttgtgcgacg tgaggtgcag cggcgtcatc gtcatgatca gaaccatcgt
     3421 ggcctggccg gccatgagcg caaccagcgc tgcgaggacc gccggacggg cgaagatctc
     3481 gcgcgccggc aacgccgggg catcgctcgc ctggcggatg ttcgtctcgt gggccagcgc
     3541 gaacggatcc gggcggagga acacgaacgt cagcagcgcg gcggcgccga cgaacagcac
     3601 cggaacgagg aacgggcccg cgtattgcgg cagcccggcg ccgagcgcca ggttgcccgc
     3661 gatcggcgcg agccagggac cgacgattcc gccgacggtc gagccccaca cgaccagccc
     3721 gatggcgacc gcccgccggt tcggcgcgac gaggtccgcc gcggcgtacc gcgataggtt
     3781 gttcgacgag ttcccgaagc cgatgatgaa ggttcccagg agcagcagtg ggaatgaccg
     3841 gatgagcacg gcttcggtgg cgatcagcgc gccgacgatg ccgaggagat agcccgtcgt
     3901 cagcccgagg cggcgaccgc gacgggccat gatcgaggag agggcaatcg cgccaacggc
     3961 tgcgccgaac acgacggctg cgcccggcgc accggcgagc gcggcggttc cgagcaggtc
     4021 ctgcccgacg atggccgcga cggtgaccgc cgcaaggtgg ccggtgctgc cgagggcgac
     4081 gcttgcgaac agggcccagc gcgtccgccg gcgcgctcgc tcgagcccgt gttcgtcgag
     4141 gtgtgcggac tcgtgggcga ggaaggacat cgcgcggatg ataggggcga gcgaccgtgc
     4201 gctagtcgaa cagggcggcg agcgcctcgt cgaaggcagc ggtgtgacgg tcgacgtcgg
     4261 cgtcggtcgt ggcgggcgac atcagcgcca tgttgtggaa cggcgtcatg aggacgccgc
     4321 ggttcatgac ccagagatgg aggagccgct ccaggtccgg atccgagttc tggatctgct
     4381 cggcgccatc gcgcgccggc tggggcatga agtggtactc cgcccgggcg ccgaggcggg
     4441 tgacgttcca cggggcgccg cggcgttcga tggcctcgtt gacaccgcgc tcccagcgtt
     4501 ccgcgagcgg gagcatccgt tcgaacgcgg ctgcagtcag gacctcgccg agggtcgccc
     4561 gcgtcgcggc catcgacagg gcgtagcccg ccagcgttcc gccgatgccc ccgacatcgg
     4621 tgtcgtcgat cgggatcgtc gcccgcactc ggtccgcaac ctctgtggtc atgccgtagg
     4681 tcgcgacggg aatgccgccg ccgatcgtct tgccgatcac cagcatgtcg ggttcgtagt
     4741 ggccggcgcc ggtgacgccg cccggaccgg cacagatggt gtgggtctcg tcgtggatga
     4801 ggatggtgcc gtggcggcgg gtgatctcgc ggacgccctc gaggtagccg gggtcgggga
     4861 gcacgatgcc gacgttggtg agggccggct cgatcaggag cgctgcgacc tggcggtccg
     4921 cgagggcccg gtcgagcgcg tcgaggtcgt tgtacggcac gacccgggtt gtcgccgccg
     4981 ggtcgacggc cgggccgagg ttgccccggc gggcgacgac gcggccgctc gcgggatcgg
     5041 cgggatccaa cacggcgaac gactcgtcga ccgagccgtg gtagcaccac tcgtggaccg
     5101 cgacgaacgg ccggtcggtg atggctcggg cgaggcggag cgagaagcgg ttggcgtcgg
     5161 tcgcggtcag ggtgaactgc cagctcggca gcccaaagcg acgcgtgagc tcctcaccga
     5221 cccacgcggc gtcctccgtc gggagcatcg tggtgatgcc ccgtcggagc tgccgctcca
     5281 ccgcggcgat cagtgcggga ggtccgtggc cggccatggc gccggtgtca ccgaggcaga
     5341 gatcgacgta gtcgtgcccg tcggcgcagg tcgcgtgcgc gcccgacgcg gactcgagga
     5401 agagcgggaa cgggccggcc catttgacca tccagttcat tggcacgccg tcgaggaggc
     5461 tggcgcgggc gcggtcgtgc agcgaacggg tccgtgggtg gcgctcggcg aacgtggcga
     5521 gctcgcgctc gagcagcaat tgaagccggg cgcggtcgac cgtcgaggtc gacggctcga
     5581 acgagggaga ggtggtcacg gccgtcagtt tgacggccgc gggctcaacc gatcaggtga
     5641 tcggcgcggc gagccctagt ccaggttctt cttgaacccg cagaccgggc aaacggcgag
     5701 gcctcgctcg gggtgcagct cgaggaagct cgtgcagccg ttggtgtggc agtagaacct
     5761 ggtgttcgtc atcgaccgtc ggcgcatgcg ccgaatcgat gcggatcgtc cgacaacgga
     5821 cgaatggtgg gtacgcgtaa gcatgtggtt tcttcgattt ccttcgtggc cgcggtcacg
     5881 ggcgagatgt gcccggatct gcggtcctgg tgctcgacgc gggttgggcg ttcgagcgat
     5941 gtacgtgcct ctggacgcgg gagctggatc gccggttcct ggactcggtc gatgctcttg
     6001 agacctgctg tccgttcatg gcatctaact tgacgccttc gagcatgggg tctcttcccc
     6061 cggcctgtca aatcctcgcc gatcagcgcg cccccgcgaa gccccccaaa ggcatggaat
     6121 cggcctgcca aaggtcatcg gatgccgcga ggtttacgag tcataaccga gcctgaagcg
     6181 ggccacggtt gcgcgttcgg ccagttcttg cgagattcac cgcggcagac tgccaattcg
     6241 ggcccgacct gagggcgtgc ccgcacgagc cgagcccgct gtgggcgggc tacgcgcaat
     6301 gacagcaatc gagctggagg cttgacgacc ttgttctcac gacgtcttcg gggagtgctt
     6361 gcgctcgcct tcgccctggc gaccgtgccg ctggccgtgc ccggcgccct cgcagccgag
     6421 ggcctcacgc tgaccacccc atacccggcg ctcaccgtga gcccgggtac ggacgtgacc
     6481 ttcaatctcg cagtcgagac gaccgcgccg gcgcgcgtcg acctgtccct gagcggcgtg
     6541 cccgcgtcct ggacggccga gatgcacggc ggcggcttcg tcgtgggcgc ggtccagacc
     6601 gacggcgaca agtcgacgga cgtccgcctc gatgtgacgg tgcccgaaga cgcgaccggc
     6661 accacccgca tcacggtgac cgcatcgagc accgggtcgg tggtcacgct gccgctggac
     6721 atcaaggtcg aggtcaacgc aggcggcgcg gtcacggtca agcctgacta cccggcccaa
     6781 cgcggtcccg cgaaccagag cttcaccttc aacctctcga tcgacaacgg caaagagcag
     6841 gatctttcgt acaacgcgac cggcgagggc ccagcgggct ggacggtcga cgtgacgctg
     6901 acgggccagg cgcaggccgt gaacggcacg gtcaaggccg gttcaactgc gagcgtcgcg
     6961 gtcaagatca cgcccgcggc gaacaccacg gcccagacct atcagaacgc agtcgtcgtg
     7021 accgttgggg acgagcagtt cccgatcgag ctcgccgtcg aggtcacggg cagctactcg
     7081 ctcgtcctgt cgacgccgac ccaattgctg tcggcccgcg gcccatccgg cagtgccacg
     7141 acgcagacct tccgaatcac gaacaccggc accgcacccg tcacaggcgt gactgtctcg
     7201 gtgaccccgc cgaccgattg gaaggtcacg ttcgctcccg agaccaccga ttcgatcgcg
     7261 cccggagcat tcgcggacat tgtcgccacg atcacaccct cgggcgatgc gatcgccggc
     7321 gactacgcgt tgaccttcac ggccaagggc aaggaagcca gcgccacgga ctccgcagag
     7381 gtgcgcttca cggtcgagag ctcgatcatc ggcgggctga tcggcgtcgt cctcatcctc
     7441 gctgccatcg gcggcctgtg gtgggtgttc cggacctacg gccgtcgctg agcgatgacc
     7501 tcctcgccaa ccaccgaggc ggctgaacgc cgcatcaagc gcaaagcgcg ccgtgagcgc
     7561 gaccgcgccc agatcgcgga gagcgacgcg cagatcatca cccgccggct gtcgaagcgc
     7621 tacgacacgc ttgtagcggt cgacaacctc gacctcgagg tgc
//
LOCUS       DATBSW010000176         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_439178, whole genome shotgun sequence.
ACCESSION   DATBSW010000176 DATBSW010000000
VERSION     DATBSW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439178"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="plsX"
                     /locus_tag="VIF63_04910"
     CDS             complement(<1..896)
                     /gene="plsX"
                     /locus_tag="VIF63_04910"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY8238756.1"
                     /translation="MPAEAATSGVRVAVDAMGGDHGPSEVIPGVLDYARTHPLDELIL
                     VGDQATVAAIAGPLPANVRIVHASQVIGMDEHPALALREKKDATILVATDLVRDGKAD
                     AVVTAGHTGAGVAAGILRLGRLPGVDRPALAVQMITESGPMVLLDIGANPNSTGENLE
                     QYARMGAIFAERVLGVAEPRVALLSIGEEKGKGDVRIAEATDRLDASGLRFIGNVEGK
                     DLTKHPADVVVCDAVLGNVVIKFFEGLSTFIFDLWRGELKRGVRGRLAYLLLRPGIAR
                     IRGTFDYETAGGSPLLGVKGTV"
     gene            complement(919..1119)
                     /gene="rpmF"
                     /locus_tag="VIF63_04915"
     CDS             complement(919..1119)
                     /gene="rpmF"
                     /locus_tag="VIF63_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY8238757.1"
                     /translation="MGVPKRRVSHARQGERRSHLAIDLPHLEECPHCHEQKRQHRVCP
                     NCGYYNGRLAIDLKPAGDDAAR"
     gene            complement(1232..1783)
                     /locus_tag="VIF63_04920"
     CDS             complement(1232..1783)
                     /locus_tag="VIF63_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY8238758.1"
                     /translation="MTQRPTDGPLTWNVAGLLTDAIGTDRQYPVVAATIEAPEGLVLT
                     SPLDGAVRFARTNRGIIVDGHLTTMLAAECSRCLKAIVVPLELDIEEEFLPSLDLATG
                     QPLPADDEPDAWRLTDHHEIDLGPVVGEAIILAEPIAPLCRPDCLGLCVTCGLPLETG
                     EHDHPDDDIDPRLEALRGFHVDA"
     gene            complement(1780..2355)
                     /locus_tag="VIF63_04925"
     CDS             complement(1780..2355)
                     /locus_tag="VIF63_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238759.1"
                     /translation="MPGRDPPSQEGTPIDIIFLVERLESLIANGKKLPLTTNVVVDQN
                     AALGLIDELRVAVPEEVRAAKRINAEGERIIEKAQEEAERIVAKAQEQAAFLIDERGL
                     THAAEAHSERLIAQAHGDADEIRRGADEYAVNVLLGLEGDVAKTLQSIKKGIALLDER
                     RAEVIAPESPDGAAEEVWADEAEDASQPLHT"
     gene            complement(2389..2883)
                     /gene="coaD"
                     /locus_tag="VIF63_04930"
     CDS             complement(2389..2883)
                     /gene="coaD"
                     /locus_tag="VIF63_04930"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8238760.1"
                     /translation="MRAAVYPGSFDPITNGHLDIIERAANVFDRVIVGVLANPRKTPL
                     LSIETRMQVIREALSAAGAPTDRIEVTAFDGLTVDFCRANDAHAIVRGLRAISDFETE
                     MQLAHNNRVLAGDIDTVFFMTAVANGYVSSSLVKEIAMFGGRIDGMVPEAAKAALTEA
                     FAGR"
     gene            complement(2883..>3137)
                     /locus_tag="VIF63_04935"
     CDS             complement(2883..>3137)
                     /locus_tag="VIF63_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY8238761.1"
                     /translation="EVGRWLTTRASGAFDIVIVDPPYDAPALIATALGGVGPWLAPGA
                     RVVVKHASTTPPPERVGLLASERERRFGETTLTFYRRQEE"
BASE COUNT          426 a         1062 c         1150 g          499 t
ORIGIN      
        1 atcacggtgc ccttcacgcc gagcagcggc gagccgccgg cggtctcgta gtcgaacgtg
       61 ccgcggatgc gggcgatgcc cgggcgcagg agcaggtagg cgagccggcc gcggacgccg
      121 cgcttgagct cgccacgcca caggtcgaag atgaaggtcg agagtccttc aaagaacttg
      181 atcacgacgt tgccgagcac cgcgtcgcac acgacgacgt cggccgggtg cttggtcagg
      241 tccttgcctt ccacgttgcc gatgaagcgc agccccgacg cgtcgaggcg atcggtggcc
      301 tcggcgatgc ggacgtcgcc cttgcccttc tcctcgccga tcgagagcag cgccacgcgc
      361 ggctccgcga cgcccaggac acgctccgcg aagatcgcgc ccatgcgcgc gtactgctcc
      421 aggttctcgc cggtggagtt ggggttggcc ccgatgtcga gcaggaccat gggtccgctc
      481 tcggtgatca tctggaccgc gagcgctggt cggtcgacgc cgggcagccg tccgaggcgc
      541 aggatcccag ccgcgacacc agccccggtg tggccggcgg tcacgacggc gtcggccttg
      601 ccgtcgcgca cgaggtcggt ggcgacgagg atcgtggcgt ccttcttctc gcggagggcg
      661 agggccggat gctcgtccat gccgatgacc tgcgaggcgt ggacgatgcg cacgttggcc
      721 ggcaggggtc cggcgatggc ggcgacggtg gcctggtcgc cgacgaggat cagctcgtcg
      781 aggggatgcg tccgggcgta gtcgaggacc cccgggatca cctcggacgg tccgtgatcg
      841 ccgcccatcg cgtccaccgc gacgcggacg ccgctggtgg cggcctcggc tggcacggac
      901 cgaggctccg tcacgcgatc agcgcgcggc gtcgtcgccg gcaggcttga ggtcgatcgc
      961 gaggcggccg ttgtagtagc cgcagttcgg gcagacgcgg tgctgccgct tctgctcgtg
     1021 gcagtgcggg cactcctcga gatgcggcag gtcgatggcg agatgcgatc ggcgctcgcc
     1081 ctggcgggca tgcgagacgc gtcgcttggg tacacccatg aggtcgtggg ctccttgtca
     1141 gatcggtctg aggtcgcgcg gcgctcgggg catcccgttc gggcagggcg tcacgccgac
     1201 ggcggagtct accgcgaaag gcgcccgacc gtcacgcatc gacgtggaag ccgcggagcg
     1261 cctcgaggcg cggatcgatg tcgtcgtcgg ggtgatcgtg ctcgcccgtc tcgagcggca
     1321 gaccgcacgt gacgcacagg ccgaggcagt ccggacggca caacggggcg atcggctcgg
     1381 ccaggatgat cgcctcgccg accaccggtc cgaggtcgat ctcgtggtgg tcggtcaggc
     1441 gccacgcatc gggttcgtcg tcggccggca ggggctggcc ggtggccagg tcgagcgacg
     1501 ggaggaactc ctcctcgatg tcgagctcga gcgggaccac gatcgccttg agacagcggc
     1561 tgcactcggc ggcgagcatc gtggtcagat ggccgtccac gatgatcccg cggttcgtgc
     1621 gcgcgaagcg gaccgcccca tcgagcgggc ttgtcaggac aaggccttcg ggtgcctcga
     1681 tggtcgccgc gacgaccgga tactggcgat cggtgccgat cgcgtcggtg agcagaccgg
     1741 cgacgttcca ggtcagcggc ccgtccgtcg ggcgctgggt catgtgtgga gcggctggga
     1801 cgcgtcctcc gcctcgtccg cccagacctc ctcggccgcg ccatcggggc tctcgggcgc
     1861 gatcacctcg gcccgccgct cgtcgagcag ggcgatgccc ttcttgatgc tctggagcgt
     1921 cttggcgaca tcgccctcga ggccgaggag cacgttgacc gcgtactcgt cggcgccgcg
     1981 ccggatctcg tcggcgtcgc cgtgggcctg cgcgatcagc cgctcgctgt gggcctcggc
     2041 ggcgtgggtc aggccccgtt cgtcgatgag gaaggcggcc tgctcctgtg ccttggcgac
     2101 gatgcgctcg gcctcttcct gggccttctc gatgatccgc tcgccttcgg cattgatccg
     2161 cttggcggca cggacctcct ccgggacggc cacgcgcagc tcgtcgatga gccccagggc
     2221 cgcgttctgg tcgacgacca cgttcgtcgt gagggggagc ttcttcccgt tggcgatgag
     2281 cgattcgaga cgctcgacga ggaagatgat gtcgatcgga gtgccctcct ggctcggcgg
     2341 gtcacgaccc ggcacgcgtg accgccagta tggcacgcgg cgagggcgtc agcgcccggc
     2401 gaacgcctcg gtcagggccg ccttggccgc ctccggaacc atcccgtcga tccgcccgcc
     2461 gaacatggcg atctccttga ccaggctcga gctgacgtag ccgttggcga ccgcggtcat
     2521 gaagaacacg gtgtcgatgt caccggccag cacccggttg ttgtgggcca gctgcatctc
     2581 cgtctcgaag tcgctgatgg cgcgcagccc gcggacgatg gcgtgcgcgt cgttggcccg
     2641 gcagaagtcg acggtcagac cgtcgaaggc cgtcacctcg atgcggtcgg tcggtgcgcc
     2701 cgcagcgctc agcgcctcgc ggatgacctg catccgcgtc tcgatcgaaa gcaacggcgt
     2761 cttgcgcggg ttggccagca cgccgacgat gacgcggtcg aagacgttcg ccgcgcgttc
     2821 gatgatgtcg aggtgcccgt tcgtgatggg atcgaacgat cccgggtaga cggccgcgcg
     2881 catcactcct cctggcgtcg gtagaaggtg agggtcgtct cgccgaaccg ccgctcgcgt
     2941 tcggatgcta gcagcccgac ccgctcgggc ggcggggtcg tcgacgcgtg cttgaccacc
     3001 acccgggcgc ccggcgcgag ccatggcccc accccgccga gcgcggtcgc gatgagcgcc
     3061 ggggcgtcgt agggcggatc gacgatgacg atgtcgaacg cgcccgatgc gcgggtcgtc
     3121 agccagcgcc ccacctc
//
LOCUS       DATBSW010000177         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_16073, whole genome shotgun sequence.
ACCESSION   DATBSW010000177 DATBSW010000000
VERSION     DATBSW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16073"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..872
                     /locus_tag="VIF63_04940"
     CDS             246..872
                     /locus_tag="VIF63_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238762.1"
                     /translation="MERRVDLRPGESPDDPDGPWIGDGWVPADPVVVPVPVTRVRVDV
                     AINADPDVTDDRRRILWRDSAMILIGLILAILAARTFLPGAAGVPTGSQAAIPSGIVI
                     GSLAPPASLAPGETFGPIIDPSLGIDATPTPIPLITLGPPTPRTTPTPTPVPTPTHRA
                     SPTPKPSKTPKPTPTLAPTPAPTLVVTPAPTDTPVPTDTPTPPTPAPT"
     gene            935..1168
                     /locus_tag="VIF63_04945"
     CDS             935..1168
                     /locus_tag="VIF63_04945"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY8238763.1"
                     /translation="MIEPGKGGAYAVLFSEIGISLLITTLVGVLAGRWVDEQLDSLPI
                     FVIVGFLIGAGSGTVMIYQLVSRFLKQIDQRPG"
     gene            1227..2258
                     /locus_tag="VIF63_04950"
     CDS             1227..2258
                     /locus_tag="VIF63_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HEY8238764.1"
                     /translation="MPDDQVATETVVSPPAPTKRRRLSPKWLALIVGVIAVDVLAFIL
                     VPPPNKEHPGEPCPFPVCFITGSLEFPAPHNIIDLAPESAAGAGGLVTFHPAISNTLF
                     TMWIVMILVIVGSWLMVRGAKLIPGRAQNFFEWFYEFLGNFGIGIAGEAGRPYLPIFL
                     GAFLLIVFDNWIGLVPPVGKIELLRAPSSDVNITLGMALVSFATFEFLGFWKLGIGGY
                     LGKFFPLYEFRNGIGAGIIALFVGLVELMLEFVKPITLSFRLFGNIYGGEVALGVITS
                     LTIVFIPVALLALELMLNAIQALIFSVLTLIFIVLAIESHHEEEGHIAEDVIAVAEGS
                     TPAAQPSHS"
     gene            2284..2505
                     /locus_tag="VIF63_04955"
     CDS             2284..2505
                     /locus_tag="VIF63_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY8238765.1"
                     /translation="MEYIGAGLAALGVIGPGIGIGILAGLSATAIGRNPDAAGQIRGL
                     AIILAAFAEGLGVLAIVVGLLAIFIQPVA"
     gene            2533..3093
                     /gene="atpF"
                     /locus_tag="VIF63_04960"
     CDS             2533..3093
                     /gene="atpF"
                     /locus_tag="VIF63_04960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY8238766.1"
                     /translation="MLFGLASAIGGDAIRLAAEAEPLFQINLFQVVIAAANFVVFLVL
                     MWQFAFKPVSKMLADRKALIEQGLRDAEQAKKDRENAEQERLAAITEARREANDILAR
                     AQKVAQETRDADIAATKTELERLRVKATDEIAAEKQRALTELRSEVADLALQAASRVV
                     GETMTDQRQRRLVEEFLADPTPGAKS"
     gene            3093..>3591
                     /gene="atpH"
                     /locus_tag="VIF63_04965"
     CDS             3093..>3591
                     /gene="atpH"
                     /locus_tag="VIF63_04965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY8238767.1"
                     /translation="MASPATTAARRYAEAAFQLATRDDKLDAYGDGLDNAAKLLGDDQ
                     ILAFLRNPARPLRERTDLVDSMLANRVPEPVLKLVGLLVERGKVDRIGTVAAEYRRQL
                     NQARGVVEAVATSATPLSSKETEALARKVTEMTGRTVDLRVAVDESLIGGLTVRVGDT
                     LYDASV"
BASE COUNT          541 a         1303 c         1173 g          574 t
ORIGIN      
        1 cgatccaccg tgttgctgat ctacgtcgtc tgcgcgacgc tcggcctgat gtcgctgctc
       61 gtgtccaacg cgacgggcgt actggcgttc ggcgtcgccc tcgtcggctt cggcgtggtg
      121 gcgttcgtcc tccgccgcaa cgcgggtgac gcgacctcgt gaccggtccg ggccgctatc
      181 cgccgccgcg gtggctgcag aggttacggt cgctcaatga ccccgaggag ctgtcgccgg
      241 aggaggtgga gcgccgggtc gacctgcggc ccggggaatc acctgacgac cccgacgggc
      301 cctggatcgg tgacggctgg gtgcccgccg acccggtggt cgtccccgtg ccggtcacga
      361 gggtgcgtgt cgacgtcgcc atcaacgctg atcccgacgt caccgacgac cgtcgccgga
      421 tcctgtggcg cgactcggcg atgatcctga tcggcctgat cctcgcgatc ctcgccgcca
      481 ggacattcct gcccggcgca gcgggggtcc cgaccggctc gcaggcggcc atccccagcg
      541 gcatcgtgat cggctcgctc gcaccgccgg ccagccttgc acccggcgaa acgttcggtc
      601 cgatcatcga tccgagcctc ggcatcgacg cgaccccgac gccgatcccg ttgatcaccc
      661 tgggcccgcc aaccccgagg accacgccga cgccgacgcc cgtgccaacg ccaactcaca
      721 gggcttcgcc caccccgaag ccgtcgaaga ccccgaagcc gactccgacg ctcgcgccga
      781 cgcccgcgcc aacgctcgtc gtgacgcctg ccccgacgga cacgcccgtc cccacggaca
      841 caccgacgcc gccgacgccg gcgccgacct gagcccttgc gggctgtggg atcgggactc
      901 gctggtatac tcccggggct ttgccaggtg gcgcgtgatc gagccgggga agggtggggc
      961 gtatgccgtc ctcttctccg agatcggcat ctcactgttg atcacgaccc tcgtcggcgt
     1021 gctcgccggg cgatgggtgg acgagcagct ggatagcctg ccgatcttcg tgatcgtcgg
     1081 cttcctgatc ggtgccggct caggcacggt gatgatctac cagctggtga gcaggttcct
     1141 gaaacagatc gaccagcggc ccggataggg cctcgacaga ccccgagcgc ggcggaggag
     1201 ccgcgctcct gcatgtggag ggagcgttgc cggacgatca ggtcgcgacc gagacggtcg
     1261 tgagcccgcc cgcgccaacg aaacgccgcc ggctttcgcc gaagtggctc gcgctcatcg
     1321 tcggcgtcat cgccgtcgat gtcctcgcgt tcatcctggt gccgccgccg aacaaggagc
     1381 acccgggcga gccgtgcccg ttcccggtct gcttcatcac cggctccctc gaatttccgg
     1441 caccccacaa catcatcgac ctcgcgcccg agtcggcggc tggggccggc ggtctcgtga
     1501 cgttccaccc cgccatcagc aacacgctgt tcacgatgtg gatcgtgatg atcctcgtga
     1561 tcgtgggctc gtggctcatg gtccgtggcg cgaagctgat ccccggccgc gcccagaact
     1621 tcttcgagtg gttctacgag ttcctcggca acttcggcat cggcatcgcc ggcgaggccg
     1681 gcaggccgta cctcccgatc ttcctgggag cgttcctgct gatcgtcttc gacaactgga
     1741 tcggcctcgt gccgccggtc ggcaagatcg agctgttgcg ggcgccctcg agcgacgtga
     1801 acatcaccct cgggatggcc ctggtcagct tcgcgacctt cgagttcctc ggcttctgga
     1861 agctcggcat cggcggctac ctgggcaagt tcttcccgct gtacgagttc aggaacggca
     1921 tcggcgccgg catcatcgcc ctgttcgtcg gcctcgtcga gctcatgctc gagttcgtca
     1981 agccgatcac gctgtcgttc cgactcttcg gcaacatcta cggcggcgag gttgccctcg
     2041 gggtcatcac ctcgctgacg atcgtcttca tccccgtcgc gctgctcgcg ctcgagctca
     2101 tgctcaacgc gatccaggcc ctcatcttca gcgtcctcac gctcatcttc atcgtcctgg
     2161 cgatcgaaag ccatcacgag gaggaagggc acatcgccga ggacgtgatc gccgtggccg
     2221 agggatcgac cccggccgcg caacccagtc attcatagcc aggttcaagg agggatctct
     2281 cgaatggagt acatcggcgc aggcctcgcg gcactcggcg tcatcggccc cggcatcggc
     2341 atcggcatcc tcgccgggct gtcggccacc gccatcgggc gcaacccgga tgcggcgggc
     2401 cagatccgcg gcctcgcgat catcctcgcg gcgttcgcag aaggcctcgg cgtcctcgcg
     2461 atcgtcgtcg gcctcctcgc catcttcatc cagcccgtcg cctagcggac cggcacggag
     2521 tagccgatcg tgatgctctt cgggctcgcc tccgcgatcg ggggcgacgc catccggctg
     2581 gccgccgagg cagagccgct gttccagatc aacctgttcc aggtcgtcat cgcggcggcc
     2641 aacttcgtcg tgttcctcgt cctgatgtgg caattcgcgt tcaagcccgt ctcgaagatg
     2701 ctcgcggacc gcaaggcgct gatcgagcag ggcctgcgcg acgccgagca ggcgaagaag
     2761 gaccgcgaga acgcggagca ggaacgcctg gccgcgatca ccgaggcccg gcgcgaggcg
     2821 aacgacatcc tggcgcgcgc ccagaaggtc gcccaggaga cccgcgacgc ggacattgcc
     2881 gcgaccaaga cggagctcga gcgcttgcgc gtgaaggcca ccgacgagat cgcggcggag
     2941 aagcagcgcg ccctgaccga attgcggagc gaggtcgccg acctggccct ccaggcggcg
     3001 tcgcgcgtcg tcggcgagac catgaccgac cagcgccagc ggcggctcgt cgaggagttc
     3061 ctcgccgatc cgaccccggg cgcgaagagc tgatggccag cccggccacg acggccgccc
     3121 ggcgctacgc cgaggccgcg ttccagctcg cgacgcgcga cgacaagctc gacgcctacg
     3181 gcgacgggct ggacaacgcg gccaagctgc tgggcgacga ccagatcctg gcgttcctgc
     3241 gcaatccggc ccggccgctg cgcgagcgga cggacctcgt cgactccatg ctcgcgaacc
     3301 gcgtcccgga gcccgtgctc aagctcgtcg ggctcctcgt cgagcgcggc aaggtcgatc
     3361 gcatcgggac cgtcgccgcg gagtaccgcc gccagctgaa ccaggcgcgc ggcgtcgtcg
     3421 aggcagtcgc gacgtcggcc acgccattgt ccagcaagga gaccgaggcg ctcgcgcgca
     3481 aggtcaccga gatgaccggt cgaaccgtcg accttcgggt cgcggtcgac gaatcgctga
     3541 tcggcgggct caccgtccgc gtcggcgaca ccctgtatga cgccagcgtc c
//
LOCUS       DATBSW010000178         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_368773, whole genome shotgun sequence.
ACCESSION   DATBSW010000178 DATBSW010000000
VERSION     DATBSW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368773"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VIF63_04970"
     CDS             <1..815
                     /locus_tag="VIF63_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238768.1"
                     /translation="VVNLASYLALAGDRVLVVDLDPQGNATSAFGIDRTRDRSVYDAV
                     IDQVQLEELAIDTAVEGVTLIPSSIALAGAEVELTPLEQRERRLATLLDPMRAKFDYL
                     LIDCPPSLGLLTINALTAADSTLIPIQCEYFALEGLGQLLATIRLVTDHLNPRLAIKG
                     AVLTMFDGRTRLSTEVGEEVRRHLGDRVYDTVIPRNVRLSEAPSHGLPIHLYAPGSRG
                     ADAYRDLADEIRARDERPPAVGSQSGSLVGLASGLPAAQRADTGQPVEAVLA"
     gene            812..1675
                     /locus_tag="VIF63_04975"
     CDS             812..1675
                     /locus_tag="VIF63_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8238769.1"
                     /translation="MTVRSDRPQGLGRGLAALIPQRSASTGGSIEIPLARIRENPRQP
                     RLRMNDEALDSLTESIRQHGVIQPVLVTETIDGYQLVAGERRVRAARMAGLERIPAIV
                     RQLADRQQLELALVENLQREDLDPIEAARGYRQLIEEFAFSQEDLASRVGRARSTVAN
                     TLRLLDLHQSVQDAIADGLVTEGHARALGGLPVDGQARVIGMVIDEGLSVRQTEELVR
                     RIREPRDGATSATPTATDPEVERVEEDLRRSLGTKVRLTKTRRGGRIVIDYYGDEELA
                     RLYVRLVGGNA"
     gene            1672..>3159
                     /locus_tag="VIF63_04980"
     CDS             1672..>3159
                     /locus_tag="VIF63_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8238770.1"
                     /translation="MTESRTGREASAAVDPAVKKTNGTGSNGASRKRAAAGEYTTASI
                     QVLEGLEAVRKRPGMYIGSTDVRGLHHLVWEVVDNSIDEAMAGHATVIQITIAKDGTV
                     EVIDDGRGIPVGKHATGKDALEVVHTVLHAGGKFGGGGYKVSGGLHGVGISVVNALSE
                     WLRVESAWDGSIWAQEYIRGKPTGPVKKIGPKGDRRGTRTQFRADPEMFETTEFSFET
                     ISQRLRESAYLTKGVWITFIDQRADRERSFYFEGGLQSFVRHLNRNKEPLHDRPIYVE
                     RREGGTMVEVALQYNDSYAENVLAFANNINTVDGGSHVTGFRAALTGSLNDWARKAGV
                     LKGSDGNLSGDDVREGLTAVISVKLTDPQFEGQTKAKLGNAEVKGQVQAAVTDSLTQY
                     LEENPGDGRKIIDKCLTASRAREAARKARDLVLRKGALDGLSLPGKLADCQERDPERS
                     EIYIVEGDSAGGSAKAGRDRRFQAILPMFGKMLNVEKARLDKVLSS"
BASE COUNT          545 a         1097 c         1070 g          447 t
ORIGIN      
        1 cggttgtcaa tctggccagc tacctggccc tcgcgggcga ccgcgtcctc gtggttgacc
       61 tcgaccccca gggcaacgcc acgagcgcgt ttggcatcga ccggacccgt gatcgatccg
      121 tgtatgacgc cgtcatcgat caggtccagc tcgaggagct ggcgatcgac acggccgttg
      181 agggcgtcac gctgattccg tcatcgatcg cgctcgccgg cgccgaggtc gagctcacgc
      241 ccctcgagca gcgcgagcgc cgccttgcga cgctgctgga tccgatgcgg gcgaagttcg
      301 actacctact gatcgactgc ccgccatcgc tcgggctgct caccatcaac gcgttgacgg
      361 ctgcggactc gacgctgatc ccgatccagt gcgagtactt cgcgctcgaa ggcctcggcc
      421 agctgcttgc gacgatccga ctcgtgaccg atcacctcaa ccctcggctc gcgatcaagg
      481 gcgccgtcct tacgatgttc gacggccgaa cgcgcctgag cacggaagtg ggcgaagagg
      541 tccgtcgaca tctcggtgat cgcgtgtacg acaccgtcat cccgcgcaac gtgcggctgt
      601 cggaggcgcc gagccacggg ctcccgatcc atctctacgc gccggggtcg cgcggcgccg
      661 acgcgtatcg cgacctcgcc gacgagattc gtgctcgcga cgagcggccg ccggccgttg
      721 gctcccagag cggttcgctt gtcggcctcg cgagcggtct gcccgccgcc cagcgagcgg
      781 acaccggcca acccgtcgag gcggtgctcg catgaccgtc cgaagcgatc ggccccaggg
      841 cctcggtcgc gggcttgctg cgctcatccc gcagcgctcc gcttccacgg gcggatcgat
      901 cgagattccg ctcgcgcgca tccgcgagaa cccgcgccag ccacgactgc gcatgaacga
      961 cgaggcgctc gacagcctca ccgagagcat ccgccagcac ggcgtgatcc agcccgtgct
     1021 cgtcaccgag acgatcgacg gctaccagct cgtggccggc gagcgccgcg tgcgcgccgc
     1081 tcggatggct gggctcgagc ggatcccggc gatcgtccgg cagctcgctg atcgccagca
     1141 gctcgagctc gcactcgtcg agaacctcca gcgcgaggac ctcgacccga tcgaggcagc
     1201 gcgcggctat cgccagctga tcgaggagtt cgcgttcagc caggaggacc tcgcctcgcg
     1261 cgtgggtcgc gcgcgatcaa ccgtggccaa caccctccgt ctcctcgacc tccaccagtc
     1321 cgtccaggac gccatcgccg atggcctcgt caccgaaggg catgcgcgcg ccctgggcgg
     1381 cctcccggtc gacggccagg cccgcgtgat cggcatggtg atcgacgaag gcctgtccgt
     1441 ccgccagacc gaggagctcg tgcggcgcat ccgcgaaccc cgcgacgggg cgacgtccgc
     1501 gacaccaaca gcgacggacc cggaggtcga gcgcgtcgag gaagaccttc ggcgttcgct
     1561 gggcacgaag gtgcgactga ccaagactcg ccgaggcggg cggatcgtga tcgactacta
     1621 cggcgacgag gagctcgcgc gcctctacgt acggctggtt ggagggaacg cgtgaccgaa
     1681 tcccgcaccg ggcgcgaggc gagcgccgca gtcgatccgg cggtcaaaaa gaccaatggg
     1741 accggcagca acggcgcgag ccgcaaacgg gccgcagccg gcgagtacac cacggccagc
     1801 atccaggtgc tggagggcct cgaggccgtc cgcaagcgcc cgggcatgta catcggctcg
     1861 acggacgtcc gcggcctcca tcacctcgtg tgggaggtcg tcgacaactc gatcgacgag
     1921 gcgatggccg gccatgccac cgtcatccag atcaccatcg cgaaggacgg cacggtcgag
     1981 gtcatcgacg acggccgcgg catcccggtg ggcaagcacg ccacgggcaa ggacgcgctc
     2041 gaggtcgtgc acaccgtcct gcatgccggc ggcaagttcg gtggcggcgg ctacaaggtc
     2101 tccggtgggc tgcacggcgt gggcatcagc gtggtcaatg ccctgtccga gtggctgcgc
     2161 gtcgagtccg cgtgggacgg gagcatctgg gcccaggaat acatccgggg caagccaacg
     2221 ggaccggtca agaagatcgg cccgaagggc gatcggcgcg gcacacgaac ccagttccgg
     2281 gccgaccccg agatgttcga gaccaccgag ttctcgttcg agacgatctc gcagcgcctc
     2341 cgtgagtccg cgtacctgac gaagggcgtg tggatcacat tcatcgacca gcgcgccgat
     2401 cgggagcggt cgttctactt cgagggcggg ctccagtcgt tcgtacggca tctgaaccga
     2461 aacaaggagc cgctccacga ccggccgatc tacgtcgagc gccgcgaggg cgggacgatg
     2521 gtcgaggtcg ccctccagta caacgactcc tacgccgaga acgtgctggc attcgccaac
     2581 aacatcaaca ccgtcgatgg cgggtcgcac gtcaccggct tccgcgccgc gctgacaggc
     2641 tcgctgaacg actgggcgcg caaggcgggc gtgctcaagg gctccgacgg caacctctcc
     2701 ggcgatgacg ttcgcgaggg gctcaccgcc gtgatcagcg tgaagctgac tgatccccag
     2761 ttcgaggggc agacgaaggc gaagctcggc aacgccgagg tcaagggcca ggtccaggcc
     2821 gcggtcacgg acagtctcac gcagtacctc gaggaaaacc ccggcgacgg ccggaagatc
     2881 atcgacaagt gcctgaccgc gtcccgagcg cgcgaggcgg cgcgcaaggc gcgcgacctc
     2941 gtgctccgca agggcgccct cgatgggctg tcgttgccgg gcaagctcgc cgattgccag
     3001 gagcgcgatc ccgagcgcag cgagatctac atcgtcgagg gcgattcggc gggtggctcg
     3061 gcgaaggcgg gccgcgatcg acgcttccag gcgatcctgc cgatgttcgg gaagatgctg
     3121 aacgtcgaga aggcacgcct cgacaaggtg ctctcaagc
//
LOCUS       DATBSW010000179         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_156953, whole genome shotgun sequence.
ACCESSION   DATBSW010000179 DATBSW010000000
VERSION     DATBSW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156953"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VIF63_04985"
     CDS             <1..592
                     /locus_tag="VIF63_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238771.1"
                     /translation="GVFTHGGHGYRHGGAEPAGADEVRTLGLGAEALRREGIEPVVVS
                     AGSTPTQLTAAAGPVTEMRAGTYVIGDRQQWAIGAVPPDGIAIAVAATVVSAAVPGQV
                     VLDAGAKTLTKDVPAYLEGHGGLPAYPDSVIARVSDYHGVVTAPDGADLPMVGEIVAV
                     IPNHVCPVIDLRDSFIATRSGSVIGRWPVDARGRSG"
     gene            702..1253
                     /locus_tag="VIF63_04990"
     CDS             702..1253
                     /locus_tag="VIF63_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8238772.1"
                     /translation="MDRDLVERARKGDREAFAVLVHQVSDSLYAVAFRILRDTGLAED
                     ALQNALVLAWRRLPKLRDSDRFEAWIHRILVHACYDESQRTRTWRTTVTVLPAELSGT
                     MDAVSGVADRDELERAYRRVPVDQRAVFVLHHYLGLPLVEVAELLGIPAGTARSRLHY
                     AIYGLRQALTADAEPITREGRLA"
     gene            1403..2224
                     /locus_tag="VIF63_04995"
     CDS             1403..2224
                     /locus_tag="VIF63_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238773.1"
                     /translation="MTNPLRVATAAVVGVVVVAGALYAISPGSPFGGPSSTPTPVPSA
                     ATPSLTPTQLPTSQPIPSLDPSASPKAVSPDTPMAAGRYYEVPFGPGGMDLCFGQAGC
                     TEDPKDEAMRFTFTVPSGWVGGSVHVLYASEGKAPPAGAGLVISRGGGLYAEPCGDEP
                     PPNILVGPTVDDFVSALVAHPKLDVTAPVDVSLAGYTGKYLDLQVPADITACSVSYFP
                     WEPGIFAQGPGSRWHLWVLDVDGIRVVIQSTDYEGTSAARRAELLAIVESIEIEP"
     gene            2284..>2739
                     /locus_tag="VIF63_05000"
     CDS             2284..>2739
                     /locus_tag="VIF63_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238774.1"
                     /translation="MYYVQHGSVPRQRHTQHRAPDGSLYAEELFGVEGFSGRSSLLYH
                     LTATTQTHKVEPVREIKLEAADENVHRHHLTKTGAVEPKGDAISGRVPLYFNGDVVFG
                     VVRPAEAMPEGTFYRNGIADEMLFVHEGTGVCDTIFGPLRYGPGDYLILP"
BASE COUNT          415 a          983 c          926 g          415 t
ORIGIN      
        1 cggcgtcttc acccacggtg gccacggcta ccggcacggc ggggccgaac cggcaggtgc
       61 tgacgaggtt cggacgctcg gcctcggcgc cgaggcgctt cgcagggaag gcatcgagcc
      121 cgtcgtcgtc agtgccggct ccacgccgac gcagctcacg gccgccgccg gacccgtcac
      181 cgagatgcgt gccggcacgt acgtgatcgg tgatcgacag cagtgggcga tcggtgcggt
      241 gccgccggac ggcatcgcca tcgcggtcgc agcgacggtc gtcagtgccg cggtgcccgg
      301 ccaggtcgtg ctcgacgccg gtgccaagac cctgaccaag gacgtccctg cgtacctcga
      361 gggtcacggc gggctgccgg cctatcccga ctccgtgatc gcgcgagtca gcgactacca
      421 cggcgtggtg acggcgccgg acggggccga cctgcccatg gtgggcgaga tcgtcgcagt
      481 gatcccgaac cacgtgtgcc ccgtgatcga cctgcgcgac tcgttcatcg cgacccggtc
      541 cggctcggtg atcggtcgat ggcctgtcga cgcgcgcggc cggagcggct gaccgcggcc
      601 gcttggaggg cgaaggcaaa cgggatctga acgttcgagg ggacaccctc cgtcagatga
      661 ggtgatcgcg gccccttcgg ccgtggccac cggaggtcga ggtggatcga gacctggtcg
      721 agcgggcccg gaagggtgac cgagaggcgt tcgcggtcct ggtgcaccag gtcagcgact
      781 cgctctacgc ggtcgccttc cgaatcctgc gcgatacggg cctcgccgag gacgcgctgc
      841 agaacgccct cgtgcttgcc tggcgccggc tgcccaagct ccgggactcc gatcgcttcg
      901 aggcctggat ccatcgcatc ctcgtccacg cctgctacga cgagtcccag cggactcgca
      961 catggcgaac gaccgtcacc gtccttccgg ccgagctgtc gggaacgatg gacgccgtgt
     1021 cgggcgtggc cgatcgcgac gagctcgagc gggcgtatcg gcgcgtgccg gtcgaccagc
     1081 gggccgtctt cgtcctgcat cactacctcg ggctgccgct cgtggaggtt gccgagctgc
     1141 tcggcatccc cgcgggcacg gcccgctcgc gactccatta cgccatctat gggcttcggc
     1201 aggccctcac ggccgacgcc gagcccatca cccgggaagg gcggctcgca tgaacgacga
     1261 tcgatcgctc gaacgcgccg ctcgatcgtg gctggaagag ggcccgacgc gggcccccga
     1321 ccggaccgtc gatgccgccc tcacccggat ccagacgaca ccacaagagc gtgaccccag
     1381 ggtcacgtgg aggttgcccg acatgaccaa cccgctccgc gtcgccactg ccgccgttgt
     1441 cggcgtcgtg gtggttgccg gcgccctgta cgccatcagc ccgggatcgc cgttcggcgg
     1501 gccttcctcg acgccgacac ccgtgccatc ggcggcgacc ccgtcgctca cacccacgca
     1561 gttgccgaca tctcagccga tcccgtcgct ggacccgtcg gcctcaccga aggccgtctc
     1621 gcccgatacg ccgatggcgg ccgggcggta ctacgaagtc ccgttcggac ccggtggaat
     1681 ggacctctgc ttcggccagg ccggatgcac ggaggacccg aaggacgagg cgatgcgctt
     1741 cacgttcacg gtccccagcg gctgggtcgg tggctcggtc catgtcctgt acgcgtccga
     1801 aggcaaggca ccccccgcgg gagccggcct cgtcatctcg cgcggcggcg gactgtatgc
     1861 cgagccatgt ggcgacgagc cgccaccgaa catcctggtt gggccgaccg tcgacgactt
     1921 cgtgagcgcc ctggtggcgc acccgaagct cgacgtcaca gcgccggtgg acgtctcgct
     1981 cgcggggtac accggcaagt atctcgacct gcaggtcccc gctgatatca cggcgtgctc
     2041 ggtgtcctac ttcccatggg aacccggcat ctttgcccag gggccgggca gtcgctggca
     2101 tctgtgggtg ctggacgtcg atggcattcg agtcgtcatc cagagcaccg attacgaggg
     2161 aacctcggcc gcgcgtcgcg ccgagctcct ggcgatcgtc gagtcgatcg agatcgagcc
     2221 ctgatcgaag gctgatcagc gtcctagact ggcctccgca taccgcggag gccagtcgat
     2281 tccatgtact acgtccagca cggcagcgtc ccccggcagc gccacaccca gcaccgggcc
     2341 cctgacggca gcctctacgc cgaggagctg ttcggggtcg agggcttcag cggccgcagc
     2401 tcgctgctct accacctgac cgcaacgacc cagacgcaca aggtcgagcc ggtccgcgag
     2461 atcaagctcg aggccgccga cgagaacgtg catcgccacc acctgacgaa gaccggcgca
     2521 gtcgagccca agggcgacgc gatcagcggg cgggtcccgc tgtacttcaa cggcgacgtc
     2581 gtgttcggcg tcgtccggcc cgccgaggcg atgcccgagg gcaccttcta ccgcaatggc
     2641 atcgccgacg agatgctgtt cgtccacgag ggcacgggcg tctgcgacac gattttcggg
     2701 ccgctgcgct acgggcccgg cgactacctc atcctgccg
//
LOCUS       DATBSW010000180         5957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_792144, whole genome shotgun sequence.
ACCESSION   DATBSW010000180 DATBSW010000000
VERSION     DATBSW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792144"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VIF63_05005"
     CDS             complement(<1..260)
                     /locus_tag="VIF63_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238775.1"
                     /translation="MFSFLFFSIKRAWQGFWRNAAMSLAATATMTLMLLLLASFWIVQ
                     AGFLASLSFVEDKVGVVADLVDDTSAADLADVQARLDAMPEVR"
     gene            complement(260..1060)
                     /gene="ftsE"
                     /locus_tag="VIF63_05010"
     CDS             complement(260..1060)
                     /gene="ftsE"
                     /locus_tag="VIF63_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY8238776.1"
                     /translation="MTLKRTRDADLDATDGPGGQMPRHDGGNGRAMRESRRPVLVLND
                     VTKTYGTGRDALKDVDLEILEGDFVFLVGASGAGKSTLMKLLIRDELATQGTVILDGD
                     DLARLPRRKVPHVRRKIGIVFQDFKLLPTKTVRENIAFALEVTGTPRRRIKPAVDRVL
                     ALVGLVDQAQQYPGTLSGGEQQRTAIARALVHDPRIVIADEPTGNLDPLISWEILQLL
                     LRINELGVTVLMATHDADIVTALRKRVVALEEGRVIRDEIGGSYHRGG"
     gene            complement(1112..2032)
                     /locus_tag="VIF63_05015"
     CDS             complement(1112..2032)
                     /locus_tag="VIF63_05015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8238777.1"
                     /translation="MTFRPATEADLSDCNRIHREGIDGYTARLGMPPSPPEENPGLRR
                     LHAHTLATDPSRFLVAERPGPDGKPRVVAFGSAVDRGPLWFLSMLFVEPGEQAHGVGT
                     ALLDRMVPEPLDGRVLATCTDSAQPVSNGLYASLGIVPRMPMFHLVGRPRYDASWPTF
                     PNGVRVERIKDATAWRTSTELAAFDRALLGFEHPEDHGFVLDEPRHCFAIRDGSDTLL
                     GYGYAGDIGRLGPLAVSDPALLAPLLGQMLTNVVPRGASAIWLQGAADEALASVIRAG
                     MRIEGFPVLIGWSRPFVDFARYVPISPGLV"
     gene            complement(2040..3089)
                     /gene="prfB"
                     /locus_tag="VIF63_05020"
                     /pseudo
     CDS             complement(2040..3089)
                     /gene="prfB"
                     /locus_tag="VIF63_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(3150..3377)
                     /locus_tag="VIF63_05025"
     CDS             complement(3150..3377)
                     /locus_tag="VIF63_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238778.1"
                     /translation="MKQAEEQTFVDLIACERDCRGCSYAAALDCDGNCGVCPHNGYCP
                     CVNPVVVRSKSALRLIELRPILLQDLRVPKN"
     gene            complement(3571..4206)
                     /locus_tag="VIF63_05030"
     CDS             complement(3571..4206)
                     /locus_tag="VIF63_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238779.1"
                     /translation="MDFNKLSDTDKRYVLLAVGVIFGGLVGVSDGWGAFATIGLLAGI
                     GVAGVILQPQLMPAMKLPIAKDQLVFGLAVVAAASFVLAGLGSLSAVFAITRIMSLLF
                     DIGLVASLALAWFAWLDFKVASPEMAAKIAAAMPDKPERAGETAPAPVAAAPTPMPAA
                     PAPEPAKEPEPEPVAAMEPETAEEMTETAAMNEPDADDDTPDASAGKPAGG"
     gene            complement(4300..4938)
                     /locus_tag="VIF63_05035"
     CDS             complement(4300..4938)
                     /locus_tag="VIF63_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY8238780.1"
                     /translation="MTPSLAGSLLRIVVFALLGALVLGSYAALQVSAQGDRDERQTAD
                     AIVVLGAAQFNGTAGGVFAARLEHAVELFDDGVAPLLIVTGGKLPGDRTTESATARRW
                     AIDHGVPAASILGEDHGRTTLESIEAVAAIMRSHDLETAVFVSDETHMLRVLRMASDQ
                     GIVAFGSATRTSPTDTDDTRRRNSMLHELAGLAAYYVGGGRLIEDPAIASAP"
     gene            complement(4967..>5957)
                     /locus_tag="VIF63_05040"
     CDS             complement(4967..>5957)
                     /locus_tag="VIF63_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY8238781.1"
                     /translation="EDDVAIESRLVSKTIESAQSRVEGFNFDIRKRVVEFDDVINKQR
                     ETIYAERDKVLRNEDLTETIRAFLDEDLDEIVERHLGSPNEAVTEEGLTDLLVDLGRM
                     GLGADRIDHDELLKLSHAGEVSEHVRDVADALLEAKEAEVGAEAWATIERFVLLRTID
                     SLWVEHLTEIDDMRRGIGLRGYAQQDPLNEFRKEAFRLYEELSGFIRHQVASTIFRVS
                     IQQPQQQQAGGVFAMQGPGQRGPGAAGAAGAARPGRAGARQGVMAGPGVSGTSGSAGM
                     AGVAGVAGAGVAGAGAAGGQQARPGYTPSGAKIGRNDACWCGSGRKYKKCHGQ"
BASE COUNT          843 a         2081 c         2106 g          927 t
ORIGIN      
        1 cggacctcgg gcatcgcgtc gagccgggcc tggacgtcgg ccaggtctgc cgcgctcgtg
       61 tcgtcgacga ggtcggcgac gaccccgacc ttgtcctcga cgaacgacag gctggcgaga
      121 aagccggcct ggacgatcca gaacgaggcc aggagcagga gcatgagcgt catcgtcgcc
      181 gtcgcggcga ggctcatcgc ggcgttgcgc cagaagccct gccaggcgcg cttgatgctg
      241 aagaagagga acgagaacat cagccgcccc ggtggtagct gccgccgatc tcgtcgcgga
      301 tcacgcggcc ctcctcgagg gcgaccacgc gcttgcggag ggcggtgacg atgtcggcgt
      361 cgtgcgtggc catgagcacg gtcacgccga gctcgttgat ccgcaggagc agctgcagga
      421 tctcccagct gatcaggggg tcgaggttgc ccgtgggctc gtcggcgatg acgatccgcg
      481 gatcgtggac gagcgcccgc gcgatcgcgg tccgctgctg ctcgccgccg gacagggtgc
      541 ccgggtactg ctgggcctgg tcgacgaggc cgacgagggc gaggacccga tcgacggccg
      601 gcttgatccg gcgacgcggc gtgcccgtga cctcgagcgc gaacgcgatg ttctcgcgca
      661 cggtcttggt cgggaggagc ttgaagtcct ggaacacgat gccgatcttg cggcgcacgt
      721 ggggcacctt gcggcgcggc agccgggcga ggtcgtcgcc gtcgaggatg accgtgccct
      781 gggtcgcgag ctcgtcccgg atcaggagct tcatcagcgt cgacttgccc gcgcccgagg
      841 cgcccacgag gaacacgaaa tcgccctcga ggatctcgag gtcgacgtcc ttgagcgcat
      901 cacggccggt gccgtaggtc ttggtcacgt cgttgaggac gagcaccggg cggcggctct
      961 cgcgcatcgc gcgaccgttc ccgccatcgt gccgcggcat ctggccgccg ggcccgtcag
     1021 tcgcgtcgag gtctgcgtct cgggtgcgtt tcagcgtcaa caacgtctcg gggaggctac
     1081 caccggcttc cagaacgcaa ctatgggccg atcagaccag tccgggggag attggtacgt
     1141 accgggcgaa atcgacgaac gggcgcgacc agccgatgag gaccgggaat ccctcgatcc
     1201 gcatccctgc ccgaatcacg ctcgcgagcg cctcgtcggc ggcgccctgg agccagatgg
     1261 ccgatgcccc gcgggggacg acgttcgtca gcatttggcc cagcagcggc gccaggagcg
     1321 ccggatccga gacggcgagc ggcccgaggc gcccgatgtc cccggcatag ccatagccca
     1381 gcagggtgtc gcttccgtcc cggatggcga agcagtggcg cggctcgtcg agcacgaagc
     1441 cgtggtcctc gggatgctcg aagcccagca gggcacgatc gaacgcagcg agctccgtgc
     1501 tcgtccgcca cgccgtggcg tccttgatcc gctcgacacg gacgccgttc gggaacgtcg
     1561 gccacgaggc gtcgtagcgc gggcgcccga cgagatggaa catcggcatc cgcggcacga
     1621 tcccgaggga cgcatagagc ccgttcgaga ccggctgagc gctgtccgtg catgtcgcca
     1681 gcacccgccc gtcgagcggt tccgggacca tccggtccag cagcgcggtg cccacgccgt
     1741 gcgcctgctc gcccggctcc acgaacagca tcgacaggaa ccacagcggc ccgcgatcca
     1801 ccgccgaccc gaacgccacg acccgcggct tgccatcggg gcccggccgc tccgccacga
     1861 ggaaccgcga agggtccgtc gcgagggtgt gggcgtgcag acgccgtagt cccgggttct
     1921 cctcgggcgg cgacggcggc atgcccaacc gtgccgtgta tccgtcgatg ccctcgcggt
     1981 ggatccggtt gcagtcggag aggtcggcct cggtcgccgg ccggaacgtc atcgcggggc
     2041 taggcatcgc tgtcgtcggc ggtcgttgcc acgcgttcga gctcggcctg catgaaggcg
     2101 tcgaggtcac cgtcgagcac ggcagcggtg ttcgaggtct cgtactgggt gcgcaggtcc
     2161 ttgaccatct ggtacgggtg caggacgtag ctccggatct ggttgcccca gccggcttcg
     2221 acgtgctcgc cccgcagctt gcgcagctcc gcctccttct cctcgagctg gcgctcgagc
     2281 aggcgcgcct tgaggacctt gatcgccgtc tccttgttct gggtctggga gcgctcgttc
     2341 tgtgactggg cgacgatccc ggtcgggagg tgggtgagcc ggaccgcgga atcggtctta
     2401 ttcacgtgct ggccgccggc cccctgcgac cggtaggtat cgacgcgaat ctcgtcccag
     2461 tccagctcga tcgggatgtc ctcgtccggc tcgggcatga cctcgaccag gccgaacgtg
     2521 gtctggcgcc ggttctgggc atcgaacggg ctgatccgca cgagccgatg gacgccgcgc
     2581 tcggcacgga gccagccgta ggcgccgcgc ccgttgaccg cgaccgagac gctcttcaga
     2641 ccggcctgct ccccctcctg ctcgtcgatg atctcggtct tccagcgatg gcgctccgcc
     2701 cagcgcaggt acatgcgcag gagcatcgcg gcccagtccg tcgcctcggt gccgccggcg
     2761 ccggcgctga tcgacaggat cgcgggccgg ccgtcgtact cgccggagaa caggagcccg
     2821 gtccgctcgc gggcgaagtc ggtctcgagg tcgtcgacgg tgcggtcgac ctcggcctcg
     2881 agctcggcgt caccggactc ctgggccagc tcgagggtgg tctcgatgtc gccggcgcgc
     2941 gcgagcaggc ccgaccagag ctcgatgtcg gcccggaggc cgtccgcctc gcgcaggacg
     3001 tcctgtgcgc cccggctgtc gtcccagaag cccggctccg acgcgcgcgc gtcgagctcg
     3061 gtcagtcgag cttgcttgcc tgggaggtca aagacgcccc gaggtcgatc ggatccgctc
     3121 cacgaggtcg cggacgcgca tggcgtccac tagttcttgg ggacccgcag gtcctggagc
     3181 aggatcggac ggagctcgat cagccgcagg gccgatttgc tccggacgac gacggggttc
     3241 acgcagggac agtagccgtt gtggggacag acgccgcaat tgccgtcgca gtcgagcgca
     3301 gcggcgtagc tgcacccccg gcaatcacgt tcacaggcga tcaggtcgac gaatgtctgt
     3361 tcctcagcct gcttcacgaa tccctcactt cggctgcggc gcggattcga ttcacgacgc
     3421 gctgcactgc ctcaggcgcg attcgcagag gtggcgctgg gcatcgcgtt ggggggtttt
     3481 tcggagtata tgtgccggat ttcgccgcgc caagagggaa aaacgcccac agagggctct
     3541 gtgggcgttt tgttgtcggc gggggaggtt tcagcccccg gctggcttcc cggcggaggc
     3601 gtccggggtg tcgtcgtcgg cgtccggctc gttcatcgcg gcggtctcgg tcatctcctc
     3661 ggcggtctcc ggctccatcg ccgcgaccgg ctccggctcg ggctccttgg cgggctccgg
     3721 ggcgggagct gcgggcatcg gcgtgggggc ggcggccacg ggcgcgggcg cggtttcccc
     3781 cgctcgctcg ggcttgtccg gcatcgcggc ggcgatcttg gcggccatct ccgggctcgc
     3841 gaccttgaag tcgagccacg cgaaccaggc gagcgcgagc gacgcaacca gcccgatgtc
     3901 gaacagcagc gacatgatcc gggtgatggc gaacacggcg ctcagcgagc ccaggccggc
     3961 caggacgaac gaggctgcgg caacgacggc gaggccgaag accagctggt ccttcgcgat
     4021 gggcagcttc atcgccggca tcagctgcgg ctgcaggatg acgcccgcca cgccgatccc
     4081 ggcgagcagc ccgatcgtgg caaaggcgcc ccacccgtcg gaaacgccca cgaggccgcc
     4141 gaagatcacg ccgacggcca gcagcacgta ccgcttgtct gtgtcgctga gcttgttgaa
     4201 atccatcgtc caccctcctc gccgttgggc gtggagcccg ccggtcacgg gtgcacggta
     4261 gcaccggacc ggacctgtat tccacgcccc cggtgacgct tagggcgcgc tcgcgatcgc
     4321 cgggtcctcg atcagccggc cgccgccgac gtagtacgcc gccagccccg cgagctcgtg
     4381 cagcatcgaa ttccgacgac gggtgtcgtc cgtgtccgtc gggctcgttc gcgtcgccga
     4441 gccgaacgcc acgatcccct ggtcggaggc catgcgcagg acccggagca tgtgggtctc
     4501 gtcgctgacg aagaccgcgg tctcgaggtc gtggctgcgc atgatcgcgg cgaccgcctc
     4561 gatggattcg agggtcgttc ggccgtgatc ctcgccgagg atcgatgcgg cgggcacgcc
     4621 gtggtcgatg gcccatcgcc gggccgtggc cgattcggtc gttcggtcgc cgggcagctt
     4681 gccgccggtg acgatcagca gcggcgcgac gccgtcgtcg aacagctcga ccgcgtgctc
     4741 caaccgcgcg gcgaacacgc cgccggcagt gccgttgaac tgggcggcac cgagcaccac
     4801 gatggcgtcg gccgtctggc gctcatcgcg atcaccctgc gccgagacct ggagtgccgc
     4861 gtagctgccg agcacgagcg cgccgagcag cgcgaagacg acgatgcgga ggagggagcc
     4921 ggcgaggctg ggagtcacgc ggcggcgtca ggcgtctgac gatcccctat tgcccgtgac
     4981 acttcttgta cttgcgcccg gagccgcacc agcaggcgtc gttgcgcccg atcttggcgc
     5041 cgctcggcgt gtagcccggt cgtgcctgct gcccgcccgc ggcgcccgca cctgccacgc
     5101 ccgcgccggc cacgccggcg accccggcca tgcccgccga gcccgaggtg cccgacacgc
     5161 ccggcccggc catgacgccc tggcgagcgc cggcccgacc cggtcgcgcg gcgcccgccg
     5221 cgcccgccgc gcccggcccg cgctggcccg ggccctgcat cgcgaacacg ccgccggcct
     5281 gctgctgctg gggctgctgg atgctgaccc ggaagatcgt gcttgcgacc tggtggcgga
     5341 tgaagcccga gagctcctcg tacaggcgga acgcctcctt ccggaactcg ttgagcggat
     5401 cctgctgggc gtagccgcgc aggccgatgc cccggcgcat gtcgtcgatc tcggtcaggt
     5461 gctcgaccca cagcgagtcg atcgtgcgca gcagcacgaa tcgctcgatc gtggcccacg
     5521 cctccgcgcc gacctcggcc tccttggcct cgagcagggc gtcggcgacg tcccggacgt
     5581 gctccgagac ctcaccggca tgcgagagct tgagcagctc gtcatggtcg atgcgatcgg
     5641 cgccgaggcc catccggccc aggtccacga gcaggtcggt gaggccctcc tcggtgacgg
     5701 cctcgttcgg gctgcccagg tggcgctcga cgatctcgtc gaggtcctcg tcgaggaacg
     5761 cccggatcgt ctcggtcagg tcctcgttgc gcagcacctt gtcgcgctcg gcgtagatcg
     5821 tctcgcgctg cttgttgatc acgtcgtcga actcgacgac ccgcttgcgg atatcgaagt
     5881 tgaagccctc gacccgggac tgggcgctct cgatggtctt ggaaacgagg cgcgactcga
     5941 tcgccacgtc gtcctcc
//
LOCUS       DATBSW010000181         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_298632, whole genome shotgun sequence.
ACCESSION   DATBSW010000181 DATBSW010000000
VERSION     DATBSW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298632"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="VIF63_05045"
     CDS             complement(<1..1770)
                     /locus_tag="VIF63_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1504..1506),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8238782.1"
                     /translation="MPKSYVRLTHPLVRDTKGGELRQATWDEALDRTVAGLRAALASK
                     ASADAKAPIGIFSCSKATNEVNYAAQKLSRQVLGSNNIDSCNRTUHAPSVAGLATVFG
                     AGGGTSSYREAEETDLIVLWGSNARETHPIFFHHLLRGVRNGARLYAVDPRRTSSAEW
                     ADVWLGLDVGSDIALANAIGREIIHAGLANMEFINRATDDFESYRAKVESYTLEYAER
                     ETGVPAEAIREMAHAYATADKAMICWTLGITEHHNAVDNVLALIDLSLLTGHVGKWGS
                     GLNPLRGQNNVQGGGDMGALPDRLPGFQYVENDVLRKRCEMFWGCDIPAKRGWHLSNM
                     LEAMDRRELTALYVIGENPMQSEADRHLTEQRLTGLDFMVVQDIFLTATAEIADVVLP
                     AAASWAESEGTVTNSERRVQRVRKAMDPPGEAREDLWIIYEIARRMGTDWGEASAEKT
                     WDEVRAISPVHRGMTYKRLEEMGGIQWPCWDEEHPGELFLHSRLWEDPVPGLRAPFVA
                     VDHDPPVDKLDADFPIRLTTGRRLDSYNTGVQSSAYTSPLRRGESLDISPEDAERFSL
                     ADGERVRIVSRRGAVETPVRIDPS"
     gene            1910..2488
                     /locus_tag="VIF63_05050"
     CDS             1910..2488
                     /locus_tag="VIF63_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238783.1"
                     /translation="MTSESAVVGTVADPSSETDDDGRMFCYRHPDRETWLRCGRCDQP
                     ICTKCSVQGPVGSRCRQCGTVRNDPLTSFTPRQLALAIGVSLVAGAVGGYIAGSIGFF
                     SIVVGYFAGRITAEAVTRVVGFKRGPVMLSIVFGGIIVGAALGAVFQFSSYYGYLPEG
                     YEVPIQVWLQSFLPGVLISAGAACFGAYQSLH"
     gene            complement(2442..>2672)
                     /locus_tag="VIF63_05055"
     CDS             complement(2442..>2672)
                     /locus_tag="VIF63_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238784.1"
                     /translation="RGDDTYRSRDYRFLGSIGWYDTVYLPLHEGENDLAIAVSEDLGG
                     WGIQARLDDLDGLDFPELSGGSGTPRSTPPRR"
BASE COUNT          369 a          908 c          936 g          459 t
ORIGIN      
        1 tgacgggtcg atgcgcaccg gggtctcgac ggcgccacga cgagacacga tccgcacccg
       61 ctcgccgtcg gcgagcgaga agcgttccgc gtcctccggc gagatgtcga gcgactcgcc
      121 gcggcgaagc ggcgaggtat acgcgctgga ctgcacgcct gtgttgtatg agtccagccg
      181 gcggccggtc gtcagccgga tcgggaagtc ggcgtcgagc ttgtcgaccg gcgggtcgtg
      241 gtcgaccgcg acgaacggcg cccggaggcc cggcacgggg tcctcccaca ggcgcgagtg
      301 caggaacagc tcgcccgggt gctcctcgtc ccagcacggc cactggatgc cgcccatctc
      361 ctcgaggcgc ttgtaggtca tgccccggtg gaccggcgag atcgcgcgga cctcgtccca
      421 tgtcttctcg gccgaggcct cgccccagtc ggtgcccatg cgccgggcga tctcgtagat
      481 gatccagagg tcctcgcgcg cctcgcccgg cgggtccatc gccttgcgca cccgctggac
      541 gcgccgctcg gagttggtga ccgtgccctc cgactccgcc caggacgcgg cggcgggcag
      601 cacgacgtcg gcgatctccg cggtcgcggt caggaagatg tcctggacga ccatgaaatc
      661 gagcccggtc aggcgttgct cggtgaggtg gcggtccgct tcagactgca tcgggttctc
      721 gccgatcacg tacagcgccg tcagctcgcg acgatccatc gcctcgagca tgttcgagag
      781 gtgccagccg cgcttcgccg ggatgtcgca gccccagaac atctcgcagc gcttgcggag
      841 cacgtcgttc tcgacgtact ggaagcccgg cagacggtcg gggagggcgc ccatgtcgcc
      901 gccgccctgg acgttgttct ggccgcgcag cgggttcagg ccgctgcccc acttcccgac
      961 atggccggtc aggagtgaca ggtcgatcag ggcgaggacg ttgtcgaccg cgttgtggtg
     1021 ctcggtgatg cccagcgtcc agcagatcat cgccttgtct gccgtcgcgt aggcgtgggc
     1081 catctcgcgg atcgcctcgg cgggcacgcc cgtctcgcgc tccgcgtact cgagcgtgta
     1141 cgactcgacc ttggcccggt acgactcgaa gtcgtccgtc gcccggttga tgaactccat
     1201 gttcgcgagg ccggcgtgga tgatctcgcg cccgatcgcg ttcgcgaggg cgatgtcgga
     1261 cccgacgtcg agacccagcc agacgtccgc ccactcggcc gagctcgtcc ggcgcgggtc
     1321 gaccgcgtac agccgggcgc cgttgcggac gccgcgcagg aggtggtgga agaagatcgg
     1381 atgcgtctcc cgggcgttgc tgccccagag cacgatcagg tcggtctctt cagcctcgcg
     1441 atatgagctg gttccgccgc cagcgccgaa cacggtcgcc agaccggcga cagagggcgc
     1501 gtgtcacgtt cggttgcagc tgtcgatgtt gttgctgccc aggacctggc gggagagctt
     1561 ctgcgccgcg tagttgacct cgttggtcgc cttggagcag ctgaagatcc cgatcggggc
     1621 cttcgcgtcg gcgctcgcct tcgaggcaag tgcggcgcgg agtcccgcga cggtccggtc
     1681 gagcgcctcg tcccaggtgg cctggcggag ctcgccgccc ttcgtgtcac gcacgagggg
     1741 atgggtcagg cgaacgtacg acttcggcaa cgatgcctcc gattcgggtt caccgccggc
     1801 ccggtggccg acccgccgag tgtaccgccg cctgtgaccc cgaccgggtc gcacacccgg
     1861 gacgaatttc aatattgacg gcggacgatc gcggcccgaa tctgccgccg tgacctctga
     1921 gagcgccgtc gtcggcaccg tggcagaccc ttcgtccgag acagacgacg acggtcggat
     1981 gttctgctac cgccatcccg accgagagac gtggctgcgc tgtggccgct gcgaccagcc
     2041 gatctgcacg aaatgctcgg tgcagggccc ggtcggatcg cgctgccgcc agtgcggcac
     2101 cgtgcgcaac gacccgctga cgtcctttac gccgcgccag ctcgcgctcg cgatcggcgt
     2161 ctcgctcgtc gccggcgcgg tcggtggcta catcgcgggt tcgatcggct tcttctcgat
     2221 cgtcgtcggg tatttcgcgg gcaggatcac cgccgaagcc gtcacgcgcg tcgtcggctt
     2281 caagcgcgga ccggtgatgc tgtcgatcgt cttcggcgga atcatcgtcg gggcagcgct
     2341 cggtgcggta tttcagttct cctcgtacta cggctacctc ccggaggggt acgaggtgcc
     2401 gatccaggtg tggctgcagt cgttcctgcc gggggtgctg atcagcgccg gggcggcgtg
     2461 cttcggggcg taccagagcc tccactgagc tcgggaaagt cgaggccgtc gaggtcgtcg
     2521 aggcgggcct ggatgcccca gccgccgaga tcctccgaga ccgcgatcgc caggtcgttc
     2581 tcgccctcgt ggagcggcag gtacaccgtg tcgtaccagc cgatgcttcc gaggaagcgg
     2641 tagtcgcgtg agcgataggt gtcgtcgccg cg
//
LOCUS       DATBSW010000182         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_897718, whole genome shotgun sequence.
ACCESSION   DATBSW010000182 DATBSW010000000
VERSION     DATBSW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897718"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VIF63_05060"
     CDS             <1..516
                     /locus_tag="VIF63_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238785.1"
                     /translation="GADSVAQYVEQLRGEVYTYTVEHPNYGKAARRLYNIFRLTGLYA
                     EAAFIRELFDEPVSALYQVAAMLRALGEADDAGEAFTTEAMVAEFDGLIMSAIGALDG
                     LAEAEMVRRLLHLRGEFSRRAKEAVEDDDIDAARADAMEAVDAYFKRVLEGVPSIKEY
                     LEEVAAAHSQG"
     gene            617..1033
                     /locus_tag="VIF63_05065"
     CDS             617..1033
                     /locus_tag="VIF63_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY8238786.1"
                     /translation="MDDLYRDYILEHYRRPHNFGVLDGATISQEGANPLCGDRITMQL
                     RVRGGVIDGVGFTGRGCAISQASASLLTDEIKGKPVDTVNAMGASDVIDLLGIEISPA
                     RMKCALLSLETLQGALEPGAVTAPAADADASPASPA"
     gene            1198..2370
                     /gene="moeB"
                     /locus_tag="VIF63_05070"
     CDS             1198..2370
                     /gene="moeB"
                     /locus_tag="VIF63_05070"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY8238787.1"
                     /translation="MQTAIREISPPEAHQRHQAGATLIDVREQVEWDAGHIPGALFIP
                     LGQLDQGIAAAVPDKGTEIILQCRSGARSGRGTAQLQALGYTNVVNLAGGILQWDTDS
                     LPIEAPATLTPAQQSRYSRHLLIPEVGTNGQAKLLGSKVLIVGAGGLGSPSALYLAAA
                     GVGTMGIVDFDVVEASNLQRQVIHSTERLGDKKVDSARKTIEALNPDVKVVTYDEMLL
                     ADNVERLITGYDVILDGTDTFETRYLLNDAAVAAGIPVVHASVFRFEGQLSTFVPYEG
                     PCYRCLYPTPPPPELAPGCSVAGVLGVVPGIMGLLQANEVLKVLLGIGEPLVGRLLLF
                     DALDGAFSELRLNRDPKCPTCSDEARAARARGEWTAPTPFGADQPFVLGSFGGAAR"
     gene            2367..2639
                     /locus_tag="VIF63_05075"
     CDS             2367..2639
                     /locus_tag="VIF63_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEY8238788.1"
                     /translation="MSVVRLPSVLRAHANGERAIDVDGSTVGAAVQSLVGRHPALAEQ
                     LLTPDGDLHRFVNVYLNGRDVRYLAGLETPVEARDEIRLLPAIAGG"
     gene            2641..3678
                     /locus_tag="VIF63_05080"
     CDS             2641..3678
                     /locus_tag="VIF63_05080"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY8238789.1"
                     /translation="MTAILRPMAGGGVASAVGGQISNRNPGRGDSRAKRYDGLIESIG
                     ATPVVEIARMSPNPAVRLFAKLEAANPTGSVKDRVARALVEDLEAAGRLSPDSILLEA
                     TSGNTGIALAMIGRRKGYRVAVVMPDNVTVERRALIALHGAEVIDSPGALGSNGAIAL
                     ARDLVARDPRFVMADQYANPANPRVHEETTGPEILDAVPELDVFVAGLGTGGTLTGVG
                     RFLRRAKPGVRIIAAEPLPGEAVQGLRSLDDGFVPEIFDPTLLDDRYVVSNAEAITTA
                     RLLLEREGIFAGPSSGAILAVAGRVARTMDSGTIVALLPDGGWKYLSAGTYTKPIDEM
                     ENELEGGVSWW"
BASE COUNT          560 a         1335 c         1308 g          559 t
ORIGIN      
        1 ggcgcggatt ccgtcgcaca gtacgtcgag cagctgcgcg gcgaggtcta cacgtacacg
       61 gtcgagcatc cgaactacgg caaggccgcg cggcggctct acaatatctt ccggctcacc
      121 ggcctctacg cggaggcggc gttcatccgc gagctcttcg atgagccggt cagcgcgctg
      181 taccaggtgg cggccatgct gcgagcactc ggcgaggccg atgacgcagg ggaggcgttc
      241 acgaccgagg cgatggtcgc ggagttcgac gggctgatca tgtccgcgat cggcgcgctc
      301 gatggactgg ccgaggccga gatggtccgc cggctcctcc acctgcgcgg ggagttcagc
      361 cgccgcgcca aggaagcggt cgaggacgac gacatcgacg cggcgcgcgc cgacgccatg
      421 gaggcggtcg acgcctactt caagcgtgtc ctcgagggcg tgccgagcat caaggagtac
      481 ctcgaggagg tcgcggcggc ccactcccag ggctgagatc gaacgttgga acgcccggcg
      541 ttccacggtc ggctggcgtc aatcccaagg ttcatggtgg ggattggctg ctcggcagta
      601 gaatgcgcga gcacctatgg acgatctgta ccgcgactac atcctcgagc actatcgacg
      661 gccccacaat ttcggggtcc tcgatggcgc gaccatctcg caggagggcg ccaatcccct
      721 gtgcggtgac cggatcacga tgcagctgcg cgttcgcggc ggcgtgatcg acggcgtcgg
      781 gttcaccggc cgcggctgtg cgatcagcca ggcgtccgcg tcgctgctga ccgacgagat
      841 caagggcaag ccggtcgaca cggtcaacgc catgggcgcg agcgacgtca tcgacctgct
      901 cggcatcgag atcagccccg cccgcatgaa gtgcgcgctg ctctcgctcg agacgctcca
      961 gggcgcgctc gagcccggcg ccgtgaccgc tccagcagcc gacgcggacg cgtccccggc
     1021 gagcccggca tgaccgaccc gaccgccacg atcggcatgg gccggtgtag gtgttgctcg
     1081 ccggcctgac cgccggcgca tatcgaccgt cgcggcacct ggcctcgacg cgtcgcacct
     1141 gtctcgtcat cgccgctctc cggcgaccct cgcccccaat tggagcttct gacatcgatg
     1201 caaactgcca tccgtgagat cagcccgccc gaggcgcatc agcgccacca ggcgggcgcg
     1261 accctgatcg acgtccgcga gcaggtcgaa tgggatgccg ggcacatccc cggcgcgctg
     1321 ttcatcccgc tcggccagct cgaccaggga atcgccgccg cggtgcccga caagggcacc
     1381 gagatcatcc tgcaatgccg cagcggcgcg cgctccggcc ggggcaccgc ccagctccag
     1441 gcgctcggct acacgaacgt cgtcaacctg gccggcggga tcctccagtg ggacacggac
     1501 agcctgccga tcgaggctcc ggcgaccctg acgcccgccc agcagtcgcg ctacagccga
     1561 cacctgctga tccccgaggt cggcacgaac gggcaggcga agctgctcgg ctcgaaggtc
     1621 ctgatcgtcg gcgcgggcgg cctcggcagc ccgagcgcgc tgtacctcgc cgcggccggc
     1681 gtgggcacga tgggcatcgt cgacttcgac gtcgtcgagg cgagcaacct ccagcgccag
     1741 gtcatccact cgaccgagcg ccttggcgac aagaaggtcg actccgcccg gaagacgatc
     1801 gaggcgctca accccgacgt gaaggtcgtc acctacgacg agatgctcct cgccgacaac
     1861 gtcgagcggc tcatcaccgg ctacgacgtg atcctcgacg gaaccgacac gttcgagacc
     1921 cgctacctgc tcaacgacgc cgcggtggcg gccgggatcc cggtcgtcca cgcgtcggtg
     1981 ttccggttcg agggccagct ctcgaccttc gtgccgtacg agggcccgtg ctaccgctgc
     2041 ctgtacccga ccccaccgcc gcccgagctc gcgcccggct gctccgtggc cggcgtgctc
     2101 ggcgtggtgc ccgggatcat gggcctgctc caggccaacg aggtgctcaa ggtcctgctc
     2161 gggatcggcg agccgctcgt cgggcggctg ctcctgttcg atgccctcga tggcgcgttc
     2221 tccgagctcc ggctcaaccg cgatccgaag tgcccgacgt gctcggacga ggcccgcgcg
     2281 gctcgagcgc gaggggagtg gacggccccc acgccgttcg gcgcggacca gccgttcgtg
     2341 ctcggctcgt tcggtggtgc ggctcgatga gcgtcgtccg actgccctcg gtcctgcggg
     2401 cgcacgccaa cggcgagcgg gcgatcgacg tcgacggctc gaccgtcggc gcggccgtcc
     2461 agtcgctcgt cggccgccac ccggcgctcg ccgagcagct gctgacgccc gacggcgacc
     2521 tccatcgatt cgtcaacgtg tacctcaacg gccgcgacgt tcggtatctc gccgggctcg
     2581 agacgccggt cgaggcgcgc gacgagatcc ggctcctgcc ggccatcgca ggaggttgag
     2641 gtgaccgcga tcctccggcc gatggctggc gggggcgtcg cctcggccgt cggagggcag
     2701 atctcgaatc gcaacccagg ccgtggcgat tcgcgggcga agcgatatga cggactgatc
     2761 gagtcgatcg gcgccacgcc ggtcgtcgag atcgcccgaa tgtcgcccaa tcccgccgtt
     2821 cgcctgttcg ccaagctcga ggccgccaat ccgaccggct cggtgaagga tcgcgtcgcg
     2881 cgggcgctcg tcgaggacct cgaggctgct ggtcgcctct ccccggattc gatcctgctc
     2941 gaggcgacgt ccggcaatac cggcatcgcg ttggcgatga tcggccggcg caagggctac
     3001 cgcgtcgcgg tggtcatgcc ggacaacgtc acggtcgagc gccgcgcgct catcgcgctc
     3061 cacggcgcgg aggtcatcga ttcgcccggt gcgctcggtt cgaacggggc gatcgcgctg
     3121 gcgcgcgacc tggtggcgcg cgacccgcgc ttcgtgatgg ccgatcagta cgccaacccg
     3181 gccaaccccc gcgtgcacga ggagacgacc ggtccggaga tcctcgacgc cgttccagag
     3241 ctcgacgtgt tcgtggccgg gttgggtacc ggcgggacgc tgaccggcgt cggccggttc
     3301 ctgcgacggg ccaagccggg cgtgcgaatc atcgcggccg agccgctgcc gggcgaggcg
     3361 gtccaggggt tgcgctcgct cgacgacggg ttcgtgcccg agatcttcga tccgacgctg
     3421 ctcgacgacc gctatgtcgt ctcgaacgcc gaggcgatca ccaccgcgcg gctcctgctg
     3481 gagcgcgagg gcatctttgc agggccgtcg tcgggcgcga tcctcgcggt cgccggtcgc
     3541 gtcgcccgga cgatggactc cgggacgatc gtcgcgttgc tgccggacgg cggctggaaa
     3601 tacctctcgg cgggcacgta caccaaaccg atcgacgaga tggagaacga gctcgagggc
     3661 ggcgtcagct ggtggtagac gaggctggtc gcgcttccgc gctgacgcct gcttcccagc
     3721 cggcgccggc gaagggcacg gatgggtact acccgggcgc cg
//
LOCUS       DATBSW010000183         7158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1074100, whole genome shotgun sequence.
ACCESSION   DATBSW010000183 DATBSW010000000
VERSION     DATBSW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074100"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="VIF63_05085"
     CDS             <1..1671
                     /locus_tag="VIF63_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8238790.1"
                     /translation="PAEPAVPVSVGRMFADSIRRAGVRWAFTVPGESFLGLLEGFEAV
                     GINVVATRHEGAAAFMAEAHAQLTGRPAACVGTRAVGGTNLGIGIHTAYSDSSPMFAF
                     VGQVERAARGREGFQEIDVTKTIGGLAKWSAEPTDVPSAVQAAIDAVDHALNGRPGPV
                     VLSVAEDLLDDEVAPEQMPTLSRVPPPRPSEDEVRDVLHLLAGAERPVILAGAGVLRA
                     RTSNDLVRLAELLRVPVVASWRRGDVISNDHPLYLGMTGYGSPATVRERLANADAVLV
                     IGCRLGEITTYGWTVPSPTTRWAHVDIAPGVVAGDLPPAAITITADARLFLRAAVARL
                     ETRGVLDAVSTDARSARNAEDRAAWEAATVIDAHPWGGPGVHPGKVITTLRQLLPDDG
                     IVTTDAGSFGTWAARGFRFRRPGTFLGSTSGAMGYGLPAAIAAGLVHRDRPVVALVGD
                     GGLGMTLAEIETAVRSGLRTIVLVFDNQRYGMIRTYQDRRPGAAAVATDLGPIDFASA
                     ARALGARGVRVEDDAGFEPALRQALVADRPSVLHLVVDRRWEGVDSRP"
     gene            1668..2162
                     /locus_tag="VIF63_05090"
     CDS             1668..2162
                     /locus_tag="VIF63_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8238791.1"
                     /translation="MSGALDPYKVLGVPRSATTQQIRSAYVVRARRSHPDLVGPRGLE
                     LMRDLNEAWEVLKDATRRAEYDAANGGPPVPRSGAVRAEDDPNRPFWTGASGPPPGRP
                     SGSVLDFGIFAGWSLGEILRRDRGYLVWLRAHPEGKPYVAEIEKLLNTGTEEIVEPRR
                     GWRR"
     gene            complement(2159..2386)
                     /locus_tag="VIF63_05095"
     CDS             complement(2159..2386)
                     /locus_tag="VIF63_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238792.1"
                     /translation="MDDQPGDGARKGPNRDELDAREAARVRRRDRDAAAAARDLMRPG
                     MGKVFKQILDAQASAAKEPPKRRKRGKPAKR"
     gene            2444..3253
                     /locus_tag="VIF63_05100"
     CDS             2444..3253
                     /locus_tag="VIF63_05100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8238793.1"
                     /translation="MAAKAKSATPVSVDTLKRERAGTYRTADGRFTVESTSSGWLLLD
                     GEQTDDLGLPLTRGPFATLDGAKAAIEPARSGPAPTSDLGKRASSAPKRSGTASKSSD
                     GPTALRASTGAGTPRAAPPPVRKAPPRAKPGVVIRELRTVDGDALRALWRECGFGSLG
                     DDDKSLARLARRNPGLVLVAAEGSRVVGSALGAWDGRRGWIYHVATAKSHRRQGIASR
                     LIAEIETGLRSLGCPKVNVLVRDDADGGHELWQALGYTAGVSRQFGKQLEG"
     gene            3256..3768
                     /locus_tag="VIF63_05105"
     CDS             3256..3768
                     /locus_tag="VIF63_05105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8238794.1"
                     /translation="MSEAIGKAVSGAKAYLTEHPDEARYRDSHARARLGGGLRVSVDG
                     PGGEALKTDMPKGIGGGALAPSPGWYFRAAAAACVASLIGIRAAELGLALPPKSVEVV
                     VDSESDDRGILGLDPAIPAGALSMKVVVRLDAPKIDPSALEALARWAVDHCPVTDTAR
                     RAVPLELVLG"
     gene            complement(3770..4525)
                     /locus_tag="VIF63_05110"
     CDS             complement(3770..4525)
                     /locus_tag="VIF63_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238795.1"
                     /translation="MDDPGDPAQSTPTGNPSTSPHLADPRALTILTTEHWNLLTARSL
                     VYNEAFARSGMFLSFLSASLVVLGLISAATGFSDAFLTVAAAFLALDLFIGFATLGRI
                     VAASDEDLRYLQGMNRLRHAYLEMVPGVERYFITSQYDDFGSVAAFYGPTGSGVIRGV
                     LHGFTTAPGMIGVICAAVAGGLGAAVALLMTHDAMVGGAAGVIAFIVVFAGGSSAVVW
                     QVGGVMRRMDVMFPAPPDAVRKEPNKALGRRPD"
     gene            complement(4596..4859)
                     /locus_tag="VIF63_05115"
     CDS             complement(4596..4859)
                     /locus_tag="VIF63_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY8238796.1"
                     /translation="MADVHLPRSLVALFPGAPRRVDASGATVADVIADLDAQVPGIRN
                     RLVDAGPELRTHINVFVEGQRATLATAVAPNAVVHVIPAVSGG"
     gene            complement(4864..5946)
                     /locus_tag="VIF63_05120"
     CDS             complement(4864..5946)
                     /locus_tag="VIF63_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238797.1"
                     /translation="MSQRTALLVGTRKGLFVLDGDSSRDEWEVRGPLCDGWPIHDAII
                     DPVSGAIFAGGGSNWYGPAVWRSDDLGKTWTHSSEGITYGDDGEKVATIWSLATGAGD
                     ALYAGVEPAGLFRSGDGGATWQHVEGLTNHPSRPEWQPGNGGLILHSIVPSPTDADRV
                     WVGISSVGAFETADGGSTWHTRNAGVRAGFMPEGQQYPEFGQCVHKLVMAADGGEHLY
                     QQNHCGVYRSSDGGKQWEEITPGLPTEFGFPMGAHPRDPKTVWTIPLTEPQDGRMMPE
                     GHAAVWRTHDGGDSWVRSDEGLPRENAFVAVLREAMAVDRLDPVGVYFGTSTGQLYGS
                     RDEGRSWRLIADNLPAIWSVEAAVVG"
     gene            6028..6423
                     /locus_tag="VIF63_05125"
     CDS             6028..6423
                     /locus_tag="VIF63_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8238798.1"
                     /translation="MGTSLGRLDYLYTQSDDVAADLRYLVDVLGGEQVFAISDGGIKV
                     ALVSFGASPGILLTDHLESDRPVHVYAVEDLAAAMRELEGRGARRERSIELPPGPAVT
                     FRTPGGVRFALYEPSRPFVVESFKGRRDF"
     gene            6485..>7158
                     /locus_tag="VIF63_05130"
     CDS             6485..>7158
                     /locus_tag="VIF63_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEY8238799.1"
                     /translation="MAIAAPPFTGFTPAAIQFLADLSENNDRAWFQPRKAEFERLLKD
                     PMEQMIAALADRLEARGVPILADPKKAPFRIYRDTRFSKDKSPYKTHLGASFPWIGHE
                     VVEAGGDHEERAHGNGGYFHFQPGEMYVGGGMWQMDKPRIDAFRDLVRDEPETVREAL
                     TAPGFVAWWGDAKPHDELKRTPPGYPQDHPLAEMFRWKDVVFGRRLSDAEVLSPSLPD
                     TLAEGYA"
BASE COUNT         1026 a         2537 c         2554 g         1041 t
ORIGIN      
        1 cccgccgaac cggcggtgcc ggtgtccgtc gggcggatgt tcgccgattc gatccggcgc
       61 gccggtgtcc gttgggcgtt caccgtgccc ggggagagct tcctcggcct gctcgaaggc
      121 tttgaggcag tgggcatcaa cgtcgtcgcc acgcgccatg agggcgcggc cgcgttcatg
      181 gccgaggccc acgcccagct gacgggccgg cccgcagcct gcgtcggcac gcgggccgtc
      241 ggcgggacca acctgggcat cggtatccac accgcgtatt cggactccag cccgatgttc
      301 gcgttcgttg gccaggtcga gcgcgccgcg cgcggtcgcg aaggcttcca ggagatcgac
      361 gtcacgaaga ccatcggcgg gctggccaag tggtcggccg agccgaccga cgttcctagc
      421 gcggtgcagg cggccatcga tgccgtcgac cacgcgctca atggccggcc gggtccggtc
      481 gtgctctcgg tcgccgagga cctgctcgac gacgaggtcg cacccgagca gatgccgacg
      541 ctcagccgcg tgccgccgcc gcgcccgtcc gaggacgagg tccgcgatgt cctccacctc
      601 ctggcgggcg ctgagcgacc cgtgatcctc gccggcgcag gcgtgctgcg tgcgcgaacc
      661 tcgaacgacc tcgtgcgcct cgccgagctc ctgcgcgtcc cggtcgtcgc ctcgtggcgc
      721 cgcggcgacg tgatctcgaa cgaccacccg ctgtacctcg gcatgaccgg ctacggctcg
      781 ccggccacgg tgcgcgagcg actcgccaat gcggacgcgg tcctcgtcat cggctgccgg
      841 ctcggcgaga tcaccaccta cggctggacc gtgccgagcc cgacgacgcg ctgggcgcac
      901 gtcgacatcg cgccgggggt cgtcgcgggc gacctgccac cggcagcgat cacgatcacc
      961 gcggatgcgc gactcttcct tcgggcggcc gtcgcgcgac tcgagacgcg aggcgtcctc
     1021 gacgcggtga gcacggacgc cagatccgcc cgcaatgccg aggaccgcgc cgcgtgggaa
     1081 gccgcaaccg tgatcgacgc acatccctgg ggcggcccag gcgtgcaccc gggcaaggtg
     1141 atcacgaccc tgcgccagct gctcccggac gacggcatcg tcacgacgga cgccgggagc
     1201 ttcgggacgt gggcagcacg gggcttccgg ttccgccggc cgggcacctt cctcggctcg
     1261 acctcgggtg cgatgggcta cggcctgccc gcagcgatcg cggcggggct cgtgcatcgg
     1321 gatcgaccag tcgtcgcgct cgtcggcgac ggcggcctcg ggatgacgct ggccgagatc
     1381 gaaaccgcgg tccggagcgg cctgcggacg atcgtgctcg tgttcgacaa ccagcgctac
     1441 ggcatgatcc gtacgtacca ggatcggcgg ccgggggcag ccgccgttgc caccgacctc
     1501 gggccgatcg acttcgcgtc ggcggctcgt gcgctcggcg cgcggggcgt gcgggtcgag
     1561 gacgatgccg gcttcgagcc tgccctgcga caagccctcg tcgcggatcg accgagcgtg
     1621 ctgcacctcg tcgtcgatcg ccgatgggag ggcgtggaca gtcgcccgtg agcggggcgc
     1681 tggatccgta caaggtcctc ggcgtgcccc gcagcgcgac cacgcagcag attcggtcgg
     1741 catacgtggt tcgcgcgcgg cgttcccatc ctgacctcgt cggcccccgc ggcctggagc
     1801 tcatgcgcga cctcaacgag gcgtgggagg tgctcaagga cgcgacccgt cgagcggagt
     1861 acgacgcggc gaatggcggc ccaccggttc ctcggagcgg tgccgtgcgc gccgaggacg
     1921 accccaatcg cccgttctgg accggtgcct ccggtccacc gccgggacgg ccctcgggtt
     1981 ccgtgctcga cttcggcatc ttcgcggggt ggtccctggg cgagatcctg cgccgcgatc
     2041 gtggctatct cgtctggctc cgggcgcacc ccgaaggcaa gccgtacgtt gccgagatcg
     2101 agaagctcct caacacgggc acggaggaga tcgtcgagcc ccggcggggc tggcgccgct
     2161 agcgcttcgc gggcttccct cgcttccgtc gcttcggcgg ctccttcgcg gcgctcgcct
     2221 gggcgtcgag gatctgcttg aacaccttgc ccatgcccgg ccgcatcagg tcgcgcgccg
     2281 cggcagcggc atcgcggtcc cggcgacgga cgcgcgcagc ctcgcgagca tcgagctcgt
     2341 cccggttcgg ccccttgcgg gccccatcgc ctggttggtc gtccacgcag cgatcgtcgg
     2401 gcgcgccgcc gctggcgtca actcctccgg taggcttctg gacatggctg ccaaggcgaa
     2461 atccgccacg cctgtctctg tggacacgct gaagcgggag cgcgccggga cgtatcggac
     2521 ggcagatggg cggttcacgg tcgagagcac ctcgagcggc tggctgctgc tggatggcga
     2581 acagaccgac gatctcggcc tgccgctcac ccgcgggccg ttcgccacgc tcgatggagc
     2641 gaaggcagcg atcgagccag cccggagcgg gcccgcgccg acgtcagacc tcggcaagcg
     2701 ggcctcgagc gcgccaaagc gatcggggac ggcgtcgaag tcgtccgacg ggccaacggc
     2761 acttcgtgcg tcgaccggag cggggacgcc cagagcggcg ccaccgccgg tgcggaaggc
     2821 gccaccacgc gccaagccag gcgtcgtcat ccgcgagctc cggacggtcg atggcgacgc
     2881 cctccgtgcc ctgtggcgcg agtgcgggtt cggctcgctc ggcgatgacg acaagagcct
     2941 cgcccggctc gcgcgccgaa acccgggcct ggtcctcgtt gccgccgagg gcagccgcgt
     3001 cgtgggatcg gcgctgggtg cctgggacgg ccgtcgcggc tggatctacc acgtcgccac
     3061 cgcgaagagc caccgccggc agggcatcgc gtcccggctc atcgccgaga tcgagaccgg
     3121 cctgcgctcg ctgggctgcc cgaaggtgaa cgtgctcgtc cgcgacgacg cggatggcgg
     3181 ccacgagctg tggcaggcgc tcggctatac ggcgggcgtt tcgaggcagt tcggcaagca
     3241 gctggagggg taggcatgtc cgaagcgatc ggcaaggccg tcagcggcgc aaaggcatac
     3301 ctgaccgagc accccgacga ggcgcgctat cgcgattcac acgcccgcgc ccgtctcggt
     3361 ggcggtctgc gcgtatcggt cgatgggccc ggcggtgagg cgctcaagac ggacatgccc
     3421 aagggcatcg gcggcggggc gctggcgcct tcgccgggct ggtacttccg cgccgccgcc
     3481 gcggcgtgcg tcgcatcgct gatcgggatc cgcgccgccg agctgggcct cgcgcttccg
     3541 ccgaagagcg tcgaggtcgt cgtcgacagc gaatccgacg atcgcggcat cctgggcctc
     3601 gatccagcga tcccggccgg cgcgctgtcg atgaaggtcg tcgtccgcct tgatgcgccg
     3661 aagatcgacc catccgcgct cgaagcgctt gcccggtggg cggtcgacca ttgcccggtc
     3721 accgacaccg cgcgccgcgc cgtgccccta gagctcgtgc tcggctgact cagtcggggc
     3781 ggcgacccag ggccttgttg ggctccttcc ggacggcgtc gggaggcgcc gggaacatca
     3841 cgtccatcct gcgcatgacc ccgcccactt gccacacgac cgcgctcgag cctccggcga
     3901 agaccacgat gaaggcgatc accccggcgg cgccgccgac catcgcgtcg tgggtcatga
     3961 gcagcgccac cgccgcgccg aggccgccgg cgacggcggc gcagatgacc ccgatcatcc
     4021 cgggcgccgt agtgaagccg tggagcacgc cgcggatgac gccggagccg gtggggccgt
     4081 agaaggcggc gaccgagccg aagtcgtcgt actggctcgt gatgaagtag cgctcgacgc
     4141 cggggaccat ctcgagatag gcgtgccgca ggcggttcat gccctgcagg tagcgaaggt
     4201 cctcgtcgct ggcggcgacg atccggccga gggtcgcgaa cccgatgaac aggtcgagcg
     4261 cgaggaatgc cgccgccacc gtgaggaagg cgtcactgaa gccggtcgcc gccgagatca
     4321 ggccgaggac gacgagcgag gcggacagga aggacaggaa catgcctgac cgggcgaagg
     4381 cctcgttgta gacgagggag cgggcggtca gcaggttcca gtgctcggtc gtgaggatcg
     4441 tcagggcacg cggatcggcg aggtgtggac tcgtgctggg gttgccagtc ggcgtcgact
     4501 gagcgggatc gcctgggtcg tccatccgcg actgctcccc aaatgcgggc ggcgcctcac
     4561 cggcgaacgc cggtcccggc cgctcctcga acacgtcagc ccccggacac ggccgggatc
     4621 acgtgcacga cggcgttcgg cgcgacggcc gtcgccagcg tggcacgctg cccttcgacg
     4681 aacacgttga tgtgggtgcg cagctccgga cctgcatcga cgagccggtt gcggatcccg
     4741 ggcacctggg catcgagatc ggcgatcacg tcggcgaccg tcgcgccgct ggcatcgacg
     4801 cgccgaggtg cgcccggaaa gagcgcgacg agcgagcgcg gcaggtggac gtctgccacg
     4861 tgctcagccg acgaccgcgg cctcgaccga ccagatcgcg ggcaggttat ccgcgatcag
     4921 gcgccaggag cggccctcgt cgcgactgcc atagagctgg ccggtgctcg tcccgaaata
     4981 gacgccgacc ggatcgagcc ggtcgaccgc cattgcctcg cggagcaccg ccacgaacgc
     5041 gttctcccgc ggcagccctt cgtcgctgcg gacccaggag tcaccgccat cgtgggtgcg
     5101 ccagacggcg gcgtggccct cgggcatcat ccgaccgtcc tggggctcgg tcagagggat
     5161 cgtccagacg gtcttcgggt cgcgagggtg agcgcccatc gggaagccaa actcggtcgg
     5221 caggccgggc gtgatctcct cccactgctt cccgccatcg ctcgagcggt acacgccaca
     5281 gtggttctgc tggtacaggt gctcgccgcc gtcggcagcc atcacgagct tgtgcacgca
     5341 ctggccgaat tccgggtact gctggccctc gggcatgaat cccgcgcgga cgcccgcgtt
     5401 ccgcgtgtgc caggtcgagc cgccatctgc cgtctcgaac gcaccgaccg acgagatccc
     5461 gacccagacg cggtccgcat cggtcgggga ggggacgatc gaatggagga tcaggccgcc
     5521 gttgcctggt tgccactccg ggcggctcgg atgattggtc aggccctcga cgtgctgcca
     5581 ggtcgcgccg ccgtcgccgc tgcggaacag cccggccggc tcgacgccgg cgtagagcgc
     5641 gtcccccgcg ccggtcgcga ggctccagat cgtggcgacc ttctcgccgt cgtcgccgta
     5701 cgtgatgccc tcgctcgagt gcgtccaggt cttgccgagg tcgtcgctgc gccagacggc
     5761 cgggccgtac cagttgctgc caccaccggc gaagatcgcc ccgctcacgg gatcgatgat
     5821 cgcgtcgtgg atcggccagc catcgcacag cgggccgcgg acctcccatt cgtctcggga
     5881 gctgtcgccg tcgagcacga acaggccctt gcgcgtgccc accaggaggg cggttcgctg
     5941 ggacatctcg atcctccgat gtgatcgcgc gacctgcggc gcgcacgctt ctctggtcta
     6001 cgctcagcct acccgacgga ggcacgcatg ggcacgtcac tgggccgtct cgactacctg
     6061 tacacccaga gcgacgacgt cgccgcggac ctccgctatc tcgtcgatgt cctgggcggc
     6121 gagcaggtct tcgcgatctc cgatggcggg atcaaggtcg cgctcgtctc gttcggcgcc
     6181 tcgcccggca tcctgttgac cgaccacctc gagagcgatc gaccggtcca tgtctatgcc
     6241 gtcgaggacc tcgctgcggc gatgcgcgag ctcgagggcc gtggcgcgcg acgtgagcgt
     6301 tcgatcgagc tgccgcctgg cccggccgtg accttccgga cgcccggcgg cgttcggttc
     6361 gcgctgtacg agccgagccg gccgttcgtc gtcgagtcgt tcaagggccg acgcgacttc
     6421 tgaccgataa gccgcgcgaa agcggcgggg cgtagattcc gggccaacgc accggaggtg
     6481 ttccatggcc atcgccgcgc cgcccttcac cggcttcacg ccggcggcga tccagttcct
     6541 cgccgacctc tcggagaaca acgatcgagc ctggttccag ccgcgcaaag ccgagttcga
     6601 gcgcctgctc aaggatccga tggagcagat gatcgctgcc ctcgccgacc ggctggaggc
     6661 ccgcggcgta ccaatcctgg cggacccgaa gaaggcgccc ttccggattt atcgggacac
     6721 ccggttcagc aaggacaagt cgccgtacaa gacgcatctc ggggcgtcgt tcccgtggat
     6781 cggccacgag gtcgtcgagg cgggtggaga ccacgaggag cgagcccacg gcaacggcgg
     6841 gtacttccat ttccagccgg gcgagatgta cgtcggcggc gggatgtggc agatggacaa
     6901 gccgcggatc gatgcgttcc gagacttggt gcgcgacgag cccgagacgg ttcgcgaggc
     6961 gctcactgcg ccgggcttcg tggcgtggtg gggcgatgcc aagccgcacg acgagctgaa
     7021 gcgcacgccg cccggctatc cccaggacca cccgctcgcg gagatgttcc gctggaagga
     7081 cgtggtcttc ggccggcgcc tgtccgacgc cgaggtgctc tcgccgagcc tgcccgacac
     7141 gctcgcggag ggctacgc
//
LOCUS       DATBSW010000184         5385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_193033, whole genome shotgun sequence.
ACCESSION   DATBSW010000184 DATBSW010000000
VERSION     DATBSW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193033"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VIF63_05135"
     CDS             <1..1089
                     /locus_tag="VIF63_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY8238800.1"
                     /translation="DDYIRLASLQTLLPNSIPLEVSVPTVLDIVIGGITFTGSLIASG
                     KLMGIIPGKPIKVPGGQLVTLALALIVAAAAIYLWIGNVSIPALGVIILASLVFGITM
                     VLPIGGADMPVVISLLNSFTGTAVAMAGFVLGNDVLIIAGALVGASGAILTKLMADAM
                     NRSVLNIMIGGFGGGEGTITVGGPEGGSVRTLGMDDAAIQLAYAQSVIIVPGYGLAVA
                     QAQHAVRELAELLEARGVIVNYAIHPVAGRMPGHMNVLLAEANVPYPQLREMEDINPE
                     FDRADVALVVGANDVTNPAARRAGTPVSGMPILDVDHAKSIIVIKRSMGKGYAGIDNE
                     LYVNPKTGMLFADAKQGLADLVAAVKAL"
     gene            1114..1974
                     /locus_tag="VIF63_05140"
     CDS             1114..1974
                     /locus_tag="VIF63_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238801.1"
                     /translation="MSAPAAAPGRGLLFGAIAVLIGVGLALGAGMLINSGGPGASGAP
                     SAGPSGSAAAVVSPGPSLSPAPLDSGVLPTSVPVETPAATVTTNPTPPPTPKPTPTPN
                     TNPTIITWKVPAQEDCTGTTAGQIHVEWKVINATGITISIDGPGLYDSYPAEGSTDLP
                     YGCSIAVLAHTYTLRTTGGTGPAASSTKKVVTREGEILKFNMPNEVSCPTTLTTVAVS
                     LVYEIRAASGVQLYRNSELYATYNGKTSSNGLTVPYDCSDETEVWHLVTDGGYGAVDD
                     DTETIKRVLT"
     gene            2047..2919
                     /locus_tag="VIF63_05145"
     CDS             2047..2919
                     /locus_tag="VIF63_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238802.1"
                     /translation="MRRTRHSLLLVPLAAVLLAACSGSAPSPSPEPTPPPEAKTLLRV
                     TMQQALPPPSTFGWLPSLVITLDGRVLSGGAVPAIFPGPLVQPVIERRITPAGWARIV
                     AAARAAGLLGGARDFTGGGVAPGGMTARLEIVADGKVFDLTGDPNRIMVCVTTPCDPQ
                     PGSPEAFGSFLSRLTELVSLVGEGNLGKEGVYVATGYALIVGSPPDDQGLAQPPMAWP
                     LAAGFAAFGKPLANGSGDRCGTITGTDADSIRAALGQATQITKFRDPVDGSFHGLIVR
                     PLLPGDGDPCEGLV"
     gene            2974..4623
                     /locus_tag="VIF63_05150"
     CDS             2974..4623
                     /locus_tag="VIF63_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEY8238803.1"
                     /translation="MDRRRPLRRGSAVALVACVAVAGLAVAPVQALAFTFHVNTIFDG
                     IDVDLANPACLTEEGKCSLRAAVMQANALDPVAGHHTINVPAGTYSLILVGTGEDDAQ
                     TGDLDIRQDMTIHGAGSSKTIIRMAPNATDDGNDRIFHVPDEDIDLVIEGATLRQGDS
                     HVQGGAAILVDEASDEVTLDRVVVRNNVAKGAIIETVGSLLIRDSSIINNDSVDATIS
                     HGYGNLTIRRSTISGNHQGGGGAVMYGGSGTGLIDRTTISGNTRVGTGGALAIMLGFS
                     AADPTSLTLRNSTIAGNNGVHAAISVRLANDMTIESTTIAGNDGIGLYSSTTTTLRNT
                     LLSNSSGNCDGGSPLPDSLGHNLDTGTSCGLDGPGDIQDGVALLQVLDYNGGSTRTRA
                     LTIGSQAIDAGFDCPGVDQRGFSRPKDGDGNGSAKCDIGAYEAAKFVEPTVEPTIEPT
                     VEPTVEPTVAPPSAAPTIEPGASGPAASAGASLEPGVSPAFSLCPCGSAPGATAPPAA
                     QPAGDDDGEISILWIVIAVLAVLLAFSLGMGLRARRNPSDG"
     gene            complement(4642..5139)
                     /locus_tag="VIF63_05155"
     CDS             complement(4642..5139)
                     /locus_tag="VIF63_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8238804.1"
                     /translation="MTNGDATDPATTPAIDAAASLGVAHRVVRTEAANSAEESASMQG
                     IPLGALLRTIVVRRGEDDYLFVLVPAGRRFDWPKLRGHLGVTRLSLPDADEAQRVTGY
                     VRYTITPFGSTRAWPVIADAAFRDQPVVAIGGGARGVNLHLAPADLVAALSATVVDVS
                     IPDPA"
     gene            complement(5136..>5385)
                     /locus_tag="VIF63_05160"
     CDS             complement(5136..>5385)
                     /locus_tag="VIF63_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8238805.1"
                     /translation="SLARQVAAQGVNVNAVAPGPVETPMFLDNTPDEIEAERRSVLIG
                     RVLQPSEIAATIAFLAGPGGDAYVGQVLSPNGGTVFPG"
BASE COUNT          848 a         1976 c         1806 g          755 t
ORIGIN      
        1 gacgactaca ttcggctggc gagcctccag accctgctgc cgaactcgat cccgctcgag
       61 gtctctgtcc cgaccgttct cgacatcgtc atcggcggca tcacgttcac gggctcgctg
      121 atcgcgtccg gcaagctcat gggcatcatc ccgggcaagc cgatcaaggt ccccggcggc
      181 cagctcgtga ccctcgccct cgccctgatc gtcgcggcgg ctgccatcta cctgtggatc
      241 ggcaacgtca gcatcccggc gctcggcgtg atcatcctcg cctcgctggt cttcgggatc
      301 acgatggtcc tgcccatcgg cggcgcggac atgcccgtcg tcatctcgct gctgaactcg
      361 ttcaccggca ccgccgtggc gatggccggc ttcgtgctcg gcaatgacgt cctgatcatc
      421 gccggcgccc tcgtcggcgc ctcgggcgcg atcctcacca agctgatggc cgacgccatg
      481 aaccgctcgg tcctgaacat catgatcggc ggcttcggcg gtggcgaagg cacgatcacg
      541 gtcggcggcc ccgagggcgg cagcgtccgg accctgggca tggacgacgc cgcgatccag
      601 ctcgcgtacg cccagtcggt gatcatcgtc ccgggctacg gcctcgcggt tgcgcaggcc
      661 cagcacgcgg tgcgcgagct cgccgagctc ctcgaggcgc gcggcgtcat cgtcaactac
      721 gcgatccacc cggtcgccgg ccggatgccc gggcacatga acgtgctgct cgccgaagcg
      781 aacgtgccgt acccacagct gcgcgagatg gaggacatca accccgagtt cgaccgagcg
      841 gatgtcgcgc tcgtggtcgg cgcgaacgac gtcacgaacc cggctgcgcg ccgcgccggc
      901 acaccggtca gcggcatgcc gatcctcgac gtcgaccacg cgaagtcgat catcgtgatc
      961 aagcgctcga tgggcaaggg ttacgcgggc atcgacaacg agctctacgt caaccccaag
     1021 acgggcatgc tcttcgccga cgcgaagcag ggcctcgcgg atctcgtggc cgccgtgaag
     1081 gcgctctagg ccgaaccgag caggaggcag cacatgtccg ctcccgcggc ggcgcccggc
     1141 cgaggcctgc tcttcggcgc gatcgcggtc ctcatcggcg tcggcctcgc gctcggcgcg
     1201 ggcatgctga tcaactcggg cgggcccggc gcaagcggtg cgcccagcgc gggtccctcg
     1261 ggcagtgccg ccgcagtcgt cagccccggc ccgtcgctct cgccggcccc actcgacagc
     1321 ggggtcctcc cgacgagcgt gccggtcgag acgcctgccg ccacggtgac gacgaatccg
     1381 acaccgccgc caacgccgaa gccgacccca accccgaaca cgaacccgac catcatcacg
     1441 tggaaggtgc ccgcccagga agactgcacc ggcacgacgg cgggccagat tcacgtcgag
     1501 tggaaggtca tcaacgcgac cgggatcacg atctcgatcg acggcccggg cctctacgac
     1561 tcgtatcccg ccgagggcag caccgacctg ccgtatggct gctcgatcgc cgtgctcgcc
     1621 cacacgtaca ccctgcgaac cacgggcggc accggccccg cggccagctc gaccaagaag
     1681 gtcgtcacgc gcgaaggcga gatcctcaag ttcaacatgc cgaacgaggt gagctgcccg
     1741 acgacgttga ccacggtcgc ggtgtcgctc gtctacgaga tccgggccgc gtcgggcgtg
     1801 cagctgtacc ggaacagcga gctctacgcg acgtacaacg gcaagacctc gagcaacggc
     1861 ctgacggtcc catacgactg ctcggacgaa acagaggtct ggcacctcgt gacggacggc
     1921 ggctacggcg ccgtcgacga cgacaccgag acaatcaagc gggtgctcac ctaggggaac
     1981 ccgtccgcgc tgctccgcgt cctccgcccg aaccaccgcc cactcactcg ccaaccgagg
     2041 acgtcgatgc gccggacccg ccattccctc ctgctcgtcc cgctcgccgc ggtgctgctc
     2101 gccgcgtgca gcggcagcgc gccatcgccg tcgcccgaac cgacgccgcc cccggaggcg
     2161 aagaccctcc tgcgggtcac gatgcagcaa gccctccctc cgccgagcac gttcggctgg
     2221 ctgccgtcgc tcgtgatcac cctcgacggt cgcgtgctga gcggcggggc agtcccggcg
     2281 atcttcccgg gtcccctggt gcaaccggtc atcgagcgcc ggatcacgcc ggccggctgg
     2341 gcccggatcg ttgccgcggc gcgcgcagcg ggcctgctcg gcggcgcccg tgacttcacc
     2401 ggcggcggcg tggcccccgg cggcatgacc gcccggctcg agatcgtcgc cgacggcaag
     2461 gtcttcgacc tcaccggcga cccgaaccgg atcatggtct gcgtgaccac tccctgcgac
     2521 ccgcagccgg gcagccccga ggcgttcggc agcttcctga gccgcctcac cgagctggtc
     2581 tcgctcgtcg gcgagggcaa tctcgggaag gagggcgtgt acgtcgcgac gggctacgcc
     2641 ctcatcgtcg gctcgccgcc cgatgaccag ggcctcgccc agccgccgat ggcctggccg
     2701 ctggccgcgg gcttcgcggc gttcggcaag ccgctggcca atggcagcgg tgatcgctgc
     2761 gggaccatca ccgggacgga cgcggactcg atccgcgcgg cgctcggcca agcgacccag
     2821 atcacgaagt tccgcgatcc ggtcgatggc tcgttccacg gcctgatcgt ccggcccctg
     2881 ctcccgggcg atggcgaccc gtgcgagggc ctcgtctgaa ccggtagcat ccaccgacac
     2941 ccgatggtcg ggtacgggga gggtgggagc gccatggatc gacgtcgtcc gctgcgtcgt
     3001 gggtccgccg tggcgctcgt cgcatgcgtc gctgttgcag gcctcgcggt ggcgccggtg
     3061 caggcgctcg cgttcacgtt ccacgtcaac acgatcttcg acggcatcga cgtcgacctc
     3121 gcgaatcccg cctgcctgac ggaggagggc aagtgctcgc tccgtgctgc ggtgatgcag
     3181 gccaacgccc tcgatccggt ggctggccac cacacgatca acgtgccggc agggacgtac
     3241 tcgctcatcc tcgtgggaac cggcgaggac gacgcgcaga ccggcgacct cgacatccgc
     3301 caggacatga cgattcacgg cgccggctcg tcgaagacga tcatccggat ggccccgaat
     3361 gccaccgacg atggcaacga ccggatcttc cacgtccccg acgaggacat cgacctcgtg
     3421 atcgagggcg cgacgctccg ccagggtgac agccacgtcc agggcggcgc cgcgatcctc
     3481 gtcgacgaag cgtcggacga ggtgacgctc gacagggtcg tggtgcggaa caacgtcgcg
     3541 aagggcgcga tcatcgagac tgtcggctcg ctcctgatcc gcgacagctc gatcatcaac
     3601 aacgactcgg tcgatgcgac gatcagccac ggctacggca acctgaccat ccgccgctcc
     3661 acgatctccg gcaaccacca gggtggcggc ggcgcggtga tgtacggcgg gagtggcacg
     3721 ggcctcatcg accgcacgac gatctcgggc aacacccgcg tgggtacggg cggcgcgctc
     3781 gcgatcatgc tggggttcag cgccgccgat ccaacgtcac tcacgctccg caacagcacg
     3841 atcgccggga acaacggcgt tcacgccgcg atcagcgttc gcctggccaa cgacatgacc
     3901 atcgagtcga cgaccatcgc aggcaacgat ggcatcgggc tctactcgtc cacgaccacg
     3961 acgcttcgca acacgctcct ctcgaactcg agcggcaact gcgacggcgg gtcaccgctc
     4021 cccgactcgc tcgggcacaa cctcgacacg ggcaccagct gcggcctcga cgggcccggt
     4081 gacatccagg acggcgtcgc gctgctccag gtcctcgatt acaacggcgg ctcgacacgg
     4141 acgcgggcgc tcaccatcgg cagccaggcg atcgatgccg gcttcgactg cccgggcgtc
     4201 gaccagcgcg ggttctcgcg acccaaggac ggcgacggca acggcagcgc gaagtgcgac
     4261 atcggggcgt acgaggcggc gaagttcgtc gagccaacgg tggagccgac gatcgagcca
     4321 accgtcgaac caacggtcga gccaacggtt gcgccaccga gcgcggcgcc gacgatcgag
     4381 ccgggcgcgt ccggtccggc ggcctcggcc ggcgcttcgc tcgagcccgg cgtgagcccg
     4441 gcgttctcgc tgtgcccatg tggcagcgcc cctggtgcga ccgcgccacc ggccgcccag
     4501 ccggcggggg acgacgatgg cgagatatcg atcctgtgga tcgtcatcgc cgtccttgcc
     4561 gtcctgctgg ctttctcgct cggcatgggg ctgcgcgcgc ggcggaatcc cagcgacggc
     4621 tgaaccgggc gcccggtccg actaggcggg atccggaatc gacacgtcga cgacggtcgc
     4681 ggacagcgcc gcgacgaggt ccgccggcgc aaggtggagg ttgacgcccc tcgcgccacc
     4741 accgatcgcg accaccggct ggtcgcggaa tgccgcatcg gcgatgacgg gccatgcccg
     4801 cgtcgagccg aacggcgtga tcgtgtagcg aacgtagccg gtgacccgct gcgcctcgtc
     4861 ggcgtcgggc agggacaggc gggtcacgcc caggtggccg cgcagcttcg gccagtcgaa
     4921 ccgccggccc gcggggacga gcacgaacag gtagtcgtcc tcgccgcggc gcacgacgat
     4981 cgtccgcagc agcgccccga gcgggatgcc ctgcatcgac gcgctttcct cggcactgtt
     5041 ggccgcctcg gtgcggacca cccgatgggc cacgccgagc gaggccgcgg catcgatggc
     5101 cggcgtcgtc gcggggtcgg tggcgtcgcc gttggtcaac ctggaaacac cgtaccgccg
     5161 ttcggcgaga gcacctggcc gacgtaggca tcgccgcccg ggccggcgag gaaggcaatc
     5221 gtcgccgcga tctcggacgg ctggaggact cgtccgatga gcaccgatct tcgttccgcc
     5281 tcgatctcgt cgggcgtgtt gtcgaggaac atcggcgtct cgacggggcc cggggcgacc
     5341 gcgttgacgt tcacgccctg ggcggcgacc tggcgagcga gcgag
//
LOCUS       DATBSW010000185         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_193073, whole genome shotgun sequence.
ACCESSION   DATBSW010000185 DATBSW010000000
VERSION     DATBSW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193073"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..460)
                     /locus_tag="VIF63_05165"
     CDS             complement(128..460)
                     /locus_tag="VIF63_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238806.1"
                     /translation="MTIFTLWFENFPNNLPALMFSLGVAVLFVVIFAKSAMSADGRSK
                     WVRKITGPNGKIFFGILFVLWAVVFGVGLQLVPHSGANTPYGGLGLIALFSGVFISMG
                     FLWSVIGE"
     gene            641..2842
                     /locus_tag="VIF63_05170"
     CDS             641..2842
                     /locus_tag="VIF63_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8238807.1"
                     /translation="MIAWAAFVVLSIPFALQTPGVLSAGGFIRDDLESAQAKALLQAE
                     IGVSEAAVVVVFHSDTARAGEPAFEAAAAAAIVDVPSAAYVRTVLSHLLSTRQVSADG
                     HTAFDIVLLDLAADESPKALPGLRAALREAPGLEIGLSGGPAFYGDVQAVSEADLRRS
                     ELISLPLAALALLLVFGSVVAAGVPLVVGGAAVVVALATIFFIAGVTPMSIFVLNLAT
                     LLGLGLGVDYSLLMTSRFREELSARGWREDAVVDAVGATARTAGRAVFFSGLTVLLGL
                     LGLVLFGFMILRSVGIAGAVVVALAVVAAMTLLPAALAIAGVNLERLRVRRVRPRPPE
                     EGAWSRLAWWVMRRPVAVLVPTLGVLLLLGTPFLHVRFNAPDARILPESAPSRAAFDR
                     LTEQFGPGPFAPIVLAIRTEGAATEPANLADLFEYSRRLAADPRIDRVDSLVDIDPRM
                     TLDQYQLLYRPGARPPDRYVAEVLSATTRGDLTGFTIFTPYGPNEAVARELVRDLRAD
                     DGALAPPAAFTVLVGGGAADVEDVVTGIAAEFPRTAIFILVSTYLVLLLLLRSLLLPL
                     KALAMNTLSIVASFGALVWIFQDGNLSLLFGTRPLGFVETTQPVILFCVLFGLSMDYE
                     VFLLTRMREAWAATGDNPSAVAHGLERSGRIVTSAALIVIVVAGSFAFADIVLIKALG
                     VGIAIAVALDATVVRALLVPATMRLLGRWNWWLPEIVARRLPMVEEAETPK"
     gene            2839..4023
                     /locus_tag="VIF63_05175"
     CDS             2839..4023
                     /locus_tag="VIF63_05175"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY8238808.1"
                     /translation="MTRRLVALLAVVVLVGGCGGAPILANEPLAFPTPAPATAPPVRP
                     ADPISIAFPRDDGPHDRLTEWWYYTGHLRSADGRRFGFEAVIFRAERGAVPAAWASHL
                     ALTDESGQRFLYAQRSEIGPQVDHSPRDAAGVPTGFDLQVNGLVAIGASVDGAAGTPV
                     APWRLSGGGGTDHIDASLAPSEATLAGGSFGLSLDLRSTKPAALHQANGFVDFGPAGS
                     SYYYSRTRLAATGQLELEGQQLEVDGIAWFDHQWGDFVSVGGGGWDWFAINLDDGTDI
                     TVSVVLDENGSPAFRYATLVTADGDVFNQESDSIEVAVIGGWKSPSTGRTYPSGWNLM
                     VGGYEIRLVPTIPDQELDTRATTGVIYWEGSQVVAAGKTGSPASAPIGGQAYVELTRY
                     GE"
     gene            4208..>4736
                     /locus_tag="VIF63_05180"
     CDS             4208..>4736
                     /locus_tag="VIF63_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8238809.1"
                     /translation="MARIKSLAMLLVIVAAACTPAVTNSGPAWTYSPAAAGASAAPGA
                     SAAPAATVGSGDPGAVVGTIELDAIDIAYEQSHVMVPKAGRYEVKLVNKGAIGHDVTF
                     ADGTQVIAAAGETTTVEVTVPAAGISFKCSVPGHADAGMTGEVMVEGATASAKPDDHG
                     GPAPSADVAADPNAPP"
BASE COUNT          635 a         1697 c         1656 g          748 t
ORIGIN      
        1 cgacgcgacg acccgctact cgcagatcgc gtcgctcgag tgggcccggc agtggccgtc
       61 cgaggaggcg tggctcgcgc gcaagcgcgc ctgatcgctg ttcggcgcgc ccgtggcggc
      121 gcgcgggcta ctccccgatc acgctccaga gaaagcccat cgagatgaac acgccgctga
      181 acagggcgat caggccgagg ccgccgtatg gggtgttcgc gccgctgtgg ggcacgagct
      241 ggaggccgac gccgaacacg acggcccaaa ggacgaacag gatgccgaag aagatcttgc
      301 cgtttgggcc ggtgatcttg cggacccact tcgagcgccc atcggccgac atcgcggact
      361 tggcgaagat cacgacgaac aggaccgcca cgcccagcga gaacatgagc gccggcaggt
      421 tgttcgggaa gttctcgaac cagagagtga agatcgtcac gctgggtcct cgtcgcgcgg
      481 cacgaccggt ggaacgggga aaatgctagc agagggctag catggtcgag ccgatgctca
      541 agcccccgcg cgccgccatg cccgcgcgcc gtccaatcca gtggcgatga gccgattcga
      601 gcgtctcggc cgatggtgcg cccggcacag atggccggtc gtgatcgcgt gggccgcgtt
      661 cgtggtgctg tccatcccgt tcgcgctcca gacaccgggc gtgctcagcg ccggcggctt
      721 catccgcgac gacctcgaat cggcgcaggc gaaagcgctg ctccaggccg agatcggcgt
      781 gtccgaggcg gcggtcgtcg tggtgttcca ctccgacacg gcacgcgccg gcgagccggc
      841 gttcgaggcg gccgcggcgg cggccatcgt cgacgtcccg agcgcggcgt acgtgcgcac
      901 cgtcctctcc cacctcctgt ccacgcgcca ggtgtcggcc gacgggcaca ccgcgttcga
      961 catcgtcctg ctcgacctcg cggccgacga gtcgccgaag gccttacccg ggttgcgtgc
     1021 cgcccttcgc gaggcgcccg ggctggagat cgggctgtcc ggcggccctg cgttctacgg
     1081 cgatgtccag gcggtatccg aggcggacct ccggcggagc gagctcatct cgctgccgtt
     1141 ggccgccctg gcgttgctgc tcgtcttcgg gtcagtcgtc gcggcgggcg tgccgctggt
     1201 cgtcggcggc gctgcggtgg tggtcgccct cgccacgatc ttcttcatcg ccggcgtcac
     1261 gccgatgagc atcttcgtgc tcaacctcgc gaccctgctc gggctgggcc tgggcgtcga
     1321 ctactcgctg ctgatgacga gccgtttccg cgaggagctg tcggcgcgcg gctggcgcga
     1381 ggacgcggtg gtcgatgccg tcggcgcgac ggcgagaacc gccgggcggg cggtgttctt
     1441 ctcggggctg accgtcctcc tcggcctcct cgggctcgtc ctgttcgggt tcatgatcct
     1501 gcgctccgtg gggattgccg gcgcggttgt cgtggcgctc gcagtcgtcg cggcgatgac
     1561 cctgttgccg gcggcgctcg cgattgccgg ggtgaacctc gagcgacttc gggtgcgccg
     1621 cgtccggccc cgaccgcccg aggagggcgc ctggtcgcgc ctcgcctggt gggtcatgcg
     1681 ccggccggtc gcggtcctcg tgcccaccct gggcgtcctg ctgttgctcg gcacgccgtt
     1741 cctgcacgtt cggttcaacg cgcccgatgc ccggatcctg ccggagagcg cgccctcgcg
     1801 cgccgcgttc gaccggctga ccgagcagtt cgggccgggc ccgttcgcgc cgatcgtgct
     1861 cgcgatccgc accgagggcg ccgccacgga gcccgcgaat ctcgccgatc tcttcgagta
     1921 ctcccggcgg ctcgcggccg acccgaggat cgaccgggtc gacagcctgg tcgacatcga
     1981 tccacggatg accctcgacc aataccagct cctgtaccgg cccggcgccc gcccgcccga
     2041 tcggtacgtc gccgaggtcc tgagcgcgac gacccggggc gacctcaccg ggttcacgat
     2101 cttcacgccc tacggcccga acgaggctgt cgcgcgcgag ctggtccgcg acctccgagc
     2161 cgacgacggc gcgctggcgc cgccggcggc gttcacggtc ctggtcggcg gtggcgcggc
     2221 cgacgtcgag gacgtcgtca cgggcatcgc cgcggagttc ccgcggaccg cgatcttcat
     2281 cctcgtcagc acgtatctcg tgctgctgct gctcctgcgc tcgctgctcc tgccgctgaa
     2341 ggcgctcgcc atgaacacgc tgtcgatcgt cgcgtcgttc ggcgcgctcg tctggatctt
     2401 ccaggacggc aacctgtcgc tcctgttcgg gacgcggccg ctcgggttcg tcgagacgac
     2461 ccaaccggtg atcctgttct gcgtcctctt cgggctgtcg atggactacg aggtgttcct
     2521 gctcacgcgc atgcgcgagg cgtgggcggc aacgggcgac aacccgagcg cggtcgcgca
     2581 cggtctcgag cgcagcggcc ggatcgtcac gtccgcggcg ctcatcgtca tcgtcgttgc
     2641 cggctcgttc gcgttcgccg acatcgtgct catcaaggcg ctcggtgtcg ggatcgcgat
     2701 cgccgtcgcg ctcgatgcga ccgtcgttcg ggcgttgctc gtgccggcga cgatgcgcct
     2761 gctcgggcgc tggaactggt ggctgccgga gatcgtggcc cgccgcctgc cgatggtcga
     2821 ggaagcggag acgcccaaat gacgaggcgc ctcgtggccc tgctcgcggt ggtcgtactc
     2881 gtcggcggct gcgggggagc gccgattctc gccaacgagc cgctcgcctt ccccaccccg
     2941 gcacccgcaa ctgctccccc ggtgcgcccc gccgacccga tctcgatcgc gttcccgcgc
     3001 gacgacgggc cccacgatcg actgaccgag tggtggtact acacgggaca cctgcgctcg
     3061 gccgatggac ggcggttcgg cttcgaggcg gtcatcttcc gcgccgagcg cggcgcggtc
     3121 cccgccgcct gggcatccca cctcgcactc accgacgaga gcggccagcg gttcctgtac
     3181 gcccagcgga gcgagatcgg accgcaggtg gatcactccc cgcgggacgc cgccggcgtc
     3241 ccgaccggct ttgacctcca ggtcaatggc ctcgtcgcaa taggcgcctc cgtcgatggc
     3301 gctgctggga cgcccgtggc gccgtggcgg ctctcgggcg ggggtggcac ggaccatatc
     3361 gacgcgagcc tcgcgccgtc cgaggcgacg ctcgcgggag gctccttcgg tctgtcactc
     3421 gacctccggt cgaccaagcc ggccgcgctg caccaggcga atgggttcgt cgacttcggc
     3481 ccggccggct cgtcctacta ctactcccga actcgcctcg ccgcgaccgg tcaactcgag
     3541 ctcgaagggc agcagctcga ggtcgacggc atcgcctggt tcgaccacca gtggggcgac
     3601 ttcgtgtccg tcgggggcgg tggctgggac tggttcgcga tcaacctcga cgacggcacc
     3661 gacatcaccg tctcggtggt gctcgacgag aacggctcgc cggcatttcg gtacgcgacg
     3721 ctcgtcacgg cagacggcga cgtgttcaac caggaatcgg attcgatcga ggtggccgtg
     3781 atcggtggct ggaagagccc ctcaaccggt cggacctatc cgagtggctg gaacctcatg
     3841 gtcggcgggt acgagatccg cctggtcccg acgatccccg accaggagct ggatacgcga
     3901 gcgaccacgg gggtcatcta ctgggagggc tcccaggtcg tggcggcggg gaagaccggc
     3961 tcgcccgcca gcgccccgat cggcgggcag gcgtacgtcg agctcacccg ctacggcgag
     4021 tagccgccga ccgcgccgaa gcgggctcgc tgtcgcgaaa acgaggggtc atgaccggcg
     4081 gcctgtccca catgggctca tcggcccttc ccccccacgc gccttcgcac aaggatggct
     4141 tcaacactcg acgtcgttcg tgaacatggc ccggccgccg ttcgggcgcc cggcaggagg
     4201 aactcttgtg gctcggatca agtcgctcgc aatgctcctc gtcatcgtcg cagcggcctg
     4261 cacgcccgcg gtaaccaact ctgggccggc ctggacgtac tcgccggccg ctgcgggagc
     4321 atcggccgcg ccgggcgcga gcgccgcgcc ggcagccacg gtcggatccg gagacccggg
     4381 cgcggtcgtc ggcacgatcg agctcgatgc gatcgacatc gcctacgagc agtcccacgt
     4441 catggtgccg aaggccggca ggtacgaggt caagctcgtc aacaagggcg cgatcgggca
     4501 cgacgtgacc ttcgccgacg gcacgcaggt catcgccgcc gcgggcgaga ccacgacggt
     4561 cgaggtcacg gtcccggccg cgggcatctc cttcaagtgc tcggtccccg gccacgcaga
     4621 tgccggcatg accggcgagg tgatggtcga gggcgcaacg gcgtccgcca agcccgatga
     4681 ccacggcggc ccggcgccgt cggccgacgt ggccgcggat ccgaatgccc caccgt
//
LOCUS       DATBSW010000186         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1251019, whole genome shotgun sequence.
ACCESSION   DATBSW010000186 DATBSW010000000
VERSION     DATBSW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251019"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VIF63_05185"
     CDS             complement(<1..544)
                     /locus_tag="VIF63_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8238810.1"
                     /translation="METSPMASSVIPRAEYAALEHGIYLNQASLGLIPRRSMQVMVEF
                     LTEIAQHGNVRLSDEAEAHVLDELRAIGAQLFDAPVRAMAVVGGASEALGQLAALTAE
                     PGGEVVLVASDFPSVTYPWLAAQARLGMAIRWVDDVPDRTLTAALIDAIRETATVVCF
                     GAVQFATGSLVDVAAVVGRAR"
     gene            637..2307
                     /locus_tag="VIF63_05190"
     CDS             637..2307
                     /locus_tag="VIF63_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238811.1"
                     /translation="MSIDMEAPRSRRAIIAGAIGGLLTAFGVSAKPSEVRAADGGNMI
                     LGATNTTTSKTEIDATTTSPGLKVLATDVALQGWGNIGVDAYGTTIGLNAVGDGTSAP
                     AVVGYNRANSTGVLGWSTDGNESTPSVPGRTGVYGLAEQVQEEGDPIARGVFGKSISG
                     IGVAAESTSGPAIDARSEESAGVIGVGPFGFVLQPLAVKPGVYGSAALEPGVYGFSTE
                     GYGLRGDSQGGDGVYGLQTPLATGVDPHVDHGPGAGVHGHGGQAGVLGTNAGGFGVQA
                     TSDSAPALFASNSSASAAAIVAEGGTGTAIHGHANPGGADQIDIPDSPPLTGLYGSAF
                     LAGTAIAADSASGLALRATSSAGHAVRGRGQLDGVIGESATDRSGVVGFSGAGAAPAG
                     PAKTGVYGEATQDATSRGVRGISLAGQGVRGEATTGQGVQGIATTGQAVRGDTTGGTA
                     VHGQSTSGTGVKAVATTGFALDVAGRATFSRSGKALIPANQAYVDVIPPGGLSGTPLV
                     FALIQYGQAGVWVTGARPGWPSASKIRIYLNKVASTTSTTPVAWFIAG"
     gene            2397..>3014
                     /locus_tag="VIF63_05195"
     CDS             2397..>3014
                     /locus_tag="VIF63_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8238812.1"
                     /translation="MTTEARPRIKITYATLRNDNEELHALYEQGVQEAKAGLGGHHQN
                     YIDGKWVDGSAGTFEARSPIDRELVVGTFAKGDRADVQRAIEAARKAAPAWRRTSWAD
                     RLTILRRAAEFISERQMRYGADMAFEVGKNRIEALGEVEEAADLIRYYAQTAEDNSFY
                     EHPFDNLGDATVHTKSILKPHGVFAVISPFNFPMALSTGPVAGALL"
BASE COUNT          497 a         1035 c         1067 g          415 t
ORIGIN      
        1 cccgggcacg cccgacgacc gccgcgacgt cgacaaggga acccgtggcg aattgcacgg
       61 cgccgaagca cacgaccgtg gcggtctccc tgatggcatc gatcaaggcg gccgtgagcg
      121 ttcggtcggg cacgtcgtcg acccatcgga tcgccatgcc aagccgcgcc tgcgccgcga
      181 gccagggata ggtcacgctc ggaaagtcgg aggcgacgag gacgacctcg ccgcccggct
      241 cggcagtgag agcggcgagc tggccaagcg cctcgctggc gcccccgacg acggccatcg
      301 cgcgcaccgg tgcgtcgaac agctgcgccc cgatcgcccg gagctcgtcg agcacgtgcg
      361 cctcggcctc gtcggacagg cggacgttgc cgtgctgggc gatctcggtc aggaactcga
      421 ccatcacctg catcgaccga cgcgggatca gcccgaggga tgcctggttc aggtagatgc
      481 cgtgctcgag cgcggcgtat tcggcgcgag ggatcaccga cgaggccatg ggggaagtct
      541 ccacgaagca tgagtgcctc gctgccttgc cccgttgccg cgacctagac tccagccagc
      601 ccggcaagcg cacacgcgat ggaggacctg tccgccatga gcatcgacat ggaagccccg
      661 cgctcccgac gagcgatcat cgcgggcgcg atcggtggcc tcctcaccgc cttcggcgtc
      721 tccgcgaagc cgagcgaggt ccgcgccgct gacggcggga acatgatcct cggcgcgacg
      781 aacacgacga cctccaagac cgagatcgac gcaaccacca caagcccagg cttaaaggtg
      841 ctcgctacgg acgtggcgtt gcaagggtgg ggaaacatcg gcgtcgatgc gtatggcaca
      901 accattggcc tgaatgccgt cggcgatggc acgtcggcgc ccgccgtagt cggctacaac
      961 cgggccaact cgaccggcgt cctgggctgg agcaccgacg gcaatgaatc gacgccgtcg
     1021 gttcccggca ggacgggcgt ctatggcctc gcggagcagg tgcaggagga gggggatccg
     1081 atcgcgcgcg gcgtgttcgg caagtcgatc tccgggatcg gcgtggcggc cgagtccacc
     1141 agtggccccg ccattgatgc gagaagcgag gagagcgcgg gcgtcatcgg cgtcggcccg
     1201 ttcgggttcg tgttgcagcc gctcgccgtg aagcccggtg tctacggctc agcggccctc
     1261 gagccaggcg tctacggctt cagcaccgag ggctacgggc ttcgcggcga ttcacagggt
     1321 ggggatgggg tctacggcct gcagaccccg ctcgccaccg gcgtcgatcc ccatgtcgac
     1381 cacgggccgg gcgcgggcgt gcacggccac ggcggccagg ccggggtgct cggcacgaac
     1441 gccggcggat tcggcgtgca ggccacgagc gacagcgcac ccgcgctctt tgcctcgaac
     1501 tcctccgcaa gtgccgcggc gatcgttgcc gagggcggta cgggaacggc catccacggt
     1561 cacgccaacc cgggcggggc agatcagatc gacatcccgg actcgccacc gctcaccggc
     1621 ctgtacgggt ctgcgttcct ggccggcacg gcaatcgccg ccgattccgc gagtggcctg
     1681 gcgctccggg cgacgagctc cgcaggtcac gcggttcgcg gccgcggcca gcttgatggc
     1741 gtgatcggcg agtcggcgac ggatcgctcc ggcgtcgtcg gcttcagcgg cgccggcgct
     1801 gctcccgcag ggccggccaa gaccggcgtc tacggcgagg cgacacagga cgcgacgtca
     1861 cgtggcgtca gggggatctc gctcgccggc cagggcgtcc gtggcgaagc gaccacgggg
     1921 cagggagtgc agggcatcgc gaccaccggt caggccgtgc gtggtgacac gaccggcggg
     1981 actgccgttc acggacagtc gacctcgggc accggcgtga aggcggtcgc gaccacagga
     2041 ttcgcgctcg acgttgcggg ccgggccacg ttcagcaggt ccggcaaggc gttgatcccg
     2101 gccaaccagg cctacgtgga cgtgatcccc ccgggtgggt tgagcgggac gccactggtg
     2161 ttcgcgctga tccagtacgg tcaggcgggt gtctgggtaa ccggtgcacg acccggctgg
     2221 ccaagcgcga gcaagatcag gatctacttg aacaaggttg cctcgacgac ctcgaccacg
     2281 ccggttgcct ggttcatcgc ggggtagcga ccgcggggtg gcagtagact gaccgaacct
     2341 tcgtgcgggt ctgattcacc cgccgcgtac cgcccacccg gcagcctgga gacccgatga
     2401 ccaccgaggc ccgtccccgc atcaagatca cctacgcgac gctccgcaac gacaacgagg
     2461 agctccacgc gctgtacgag cagggcgtcc aggaggcgaa ggccgggctc ggcggccacc
     2521 accagaacta catcgacggg aaatgggtcg acggctcggc ggggacgttc gaagcccgct
     2581 cgccgatcga tcgtgagctc gtcgtcggga cgttcgccaa gggcgaccga gccgatgtcc
     2641 agcgcgcgat cgaggccgcg cggaaagccg cgccggcgtg gcggcggacc tcctgggcgg
     2701 accggctcac gatcctccgt cgggccgccg agttcatctc cgagcgccag atgcgctacg
     2761 gcgcggacat ggcgttcgag gtcggcaaga accggatcga ggcgctcggt gaggtcgagg
     2821 aagccgcgga cctcatccgc tactacgcgc agactgccga ggacaacagc ttttacgagc
     2881 acccgttcga caacctcggc gacgcgacgg tccacacgaa gtcgatcctg aagccgcacg
     2941 gcgtgttcgc cgtcatcagc ccgttcaact tcccgatggc cctttcgacc ggcccggtcg
     3001 ctggggcgct gctg
//
LOCUS       DATBSW010000187         7128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_298998, whole genome shotgun sequence.
ACCESSION   DATBSW010000187 DATBSW010000000
VERSION     DATBSW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298998"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VIF63_05200"
     CDS             <1..906
                     /locus_tag="VIF63_05200"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEY8238813.1"
                     /translation="GSLNIDLVAYAARVPGAGETVLGDRFQMGFGGKGANQAVMAARL
                     GARVSMVGALGDDVYAGMTVDNLIAQGVDAAHVARVDGSSGVAPIWVEPDGTNRIIVV
                     AGANDAVDPATAADAVRALEGVAVVVGQLEIPQRVTAAAFVAAREVGAVTILNPAPAA
                     ALDPTLLDGADWLMPNETEFAILAGVDSFDPADDAALLAFAATIRPRLVVTLGSRGAG
                     LVTKDGTVERVPAIRVDAVDTTGAGDAFVGAFAYGLAAGLPELTAMRLGIACASDSVT
                     KTGTQSSFATPEGAGDIFDKVLGAA"
     gene            complement(1238..1537)
                     /locus_tag="VIF63_05205"
     CDS             complement(1238..1537)
                     /locus_tag="VIF63_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY8238814.1"
                     /translation="MSLPCPHFATAMDDLPAALDVCPTCVEMGETWVHLRQCLVCGRT
                     GCCDNSPNRHATAHYNEAGHALIRSAQPGEIWAWCYPDNAFFIPGEDGGWELFEE"
     gene            complement(1569..2069)
                     /locus_tag="VIF63_05210"
     CDS             complement(1569..2069)
                     /locus_tag="VIF63_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238815.1"
                     /translation="MVASLDSRGRGVLLITATPGVVVLLVLAMTLALGRAGIVSVVLG
                     LLALLYFEHGLLTNETDLFQLGLVGVAILIVGELAQWSIDSRVPGRYERGLHRSRAAA
                     IGALALLGLATVMVSGIAAGIPIGGGIESVVIAMAASVGVLGLISLVGMRSAGTATGS
                     PGRDPS"
     gene            complement(2108..3385)
                     /locus_tag="VIF63_05215"
     CDS             complement(2108..3385)
                     /locus_tag="VIF63_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8238816.1"
                     /translation="MTRRPSPRLRAYLALGAAGLIAAVAFGRVEPVILVAPLLIAALL
                     ALVLVEEPEISIQATIASSRLLEGQQVNVDLHVRAEGPVPWLELALVLPAGIVATDGV
                     RVVGLRLEAGGERDVSIAVAARRWGAYRVGEVLVRARDRFGFFAYETRLEGALALRVF
                     PKPEELRRVIRPAETQMYAGDEVSRRRGDGIEFADVRPYAAGDRIRRINWRLSSRRSE
                     LYVNELHPERSTDVVILLDTFTDIGDGEGHSSLATAVRGANGIADIYLRRRDRVGLIA
                     FGGSIRWLVPSMGLAQGYRIVEMLLGARAAATFVWKGVDLIPPRSLPPKALIIVLSPL
                     LDDRSIEAILDLLARGFDLAVIEILPEEYIAPPAPGFASTARRLWHLERELLRDRLRR
                     AGVAVAAWSSNEPLDAVLEEVRAFRRHARRASA"
     gene            complement(3382..4365)
                     /locus_tag="VIF63_05220"
     CDS             complement(3382..4365)
                     /locus_tag="VIF63_05220"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8238817.1"
                     /translation="MTDRMPIPDIAARGRAILDEVERAVVGKRDVLELFLLAVLSDGH
                     VLIDDLPGLAKTLMARSFAQVTGLQFRRIQFTPDLMPSDVTGASIYNQKTAEFVFREG
                     PVFANLLLADEINRAPAKTQSSLLESMQERQVTTEGTTRQLPRPFLVIATQNPIEYEG
                     TYPLPEAQLDRFLLRLRVGYPSGSEEATILRARIERKADEVQLKGVVDAATVSAMQAG
                     VEDVFVGESIIRYVVALVGATRASARLQVGASPRGTLALLKLSRARAVMDGRDYVVPD
                     DVKAVAVPALAHRLILRPELWVQQVRAEQVVQECLDAVPAPSAQEPAPVTS"
     gene            complement(4367..4948)
                     /locus_tag="VIF63_05225"
     CDS             complement(4367..4948)
                     /locus_tag="VIF63_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238818.1"
                     /translation="MTYEFDRSKRIAPPSPDGARPGRSPAVGREPIAFAAMVAIALVA
                     FVLAPPDAQGSVGRVAILVIGIVAAWRVLGRSDAITASTSERFELDLAPPAGQRPEIS
                     GLRSVETALRMSSGGAFGLETMLKPRLRALVRWRLQRSHAVDLDAAPEAAQRFLGDRL
                     SRLISTGGAGAAFDGPGAALDEVNLAINELEQL"
     gene            complement(4945..5856)
                     /locus_tag="VIF63_05230"
     CDS             complement(4945..5856)
                     /locus_tag="VIF63_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY8238819.1"
                     /translation="MADTEDEAPPSGATRIPGWVALPAAAGLIALVALTSRSQATGTS
                     TGPIGLESGITVLEVIGYVFLAIGIAVLPIIAVAHGRARQMRMAAAAAQPKRPPAPWW
                     ANLLGAVVVIPLIVFQVAIILNFIIELLQRRADEGTGIGGLLQDVVGRLDPSRADSPA
                     LGIAMVIVIVIFLVVVGLLIRWRLTDTRGKRIDRGRAASTVAAVEVSLEAIRREPDPR
                     RAVIAAYAAMERSLAHEGYPREESEAPLEYLRRVLAASTHAVDEVRTLTLLFQHAKFS
                     DHAVDETMRSGAITALERIRTAASASA"
     gene            complement(5974..6405)
                     /locus_tag="VIF63_05235"
     CDS             complement(5974..6405)
                     /locus_tag="VIF63_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238820.1"
                     /translation="MSALRLSSVALLAAVVLVGCSWFSRTFDFSFPAVGDIAALPVVV
                     HDQSGIIVSVDDGPPVPERPIPAGIGTVDRNPNALVVFWTGGACDESVAITVSAPPNL
                     DFMITTNRKPGGCDAIGIGRSLLIRLSEPLDMSRTSVSFDD"
     gene            complement(6405..6893)
                     /locus_tag="VIF63_05240"
     CDS             complement(6405..6893)
                     /locus_tag="VIF63_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238821.1"
                     /translation="MHGLDRARRSGGMTLAALFIAFITAACGSGGPAATSPDALLATS
                     VPATPKPTPTPKPTPTAKPTPLPMDVTKEAFTAKVATTKTASVTIRTEPSARCTIEVL
                     YDSGPSQAAGLDPKKATSGGKVIWSWKVGSNTKAGTYPITIVCTLDDHEGTLELDFTV
                     TP"
     gene            complement(6952..7037)
                     /locus_tag="VIF63_05245"
     tRNA            complement(6952..7037)
                     /locus_tag="VIF63_05245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7001..7003),aa:Ser,seq:tga)
BASE COUNT         1127 a         2407 c         2525 g         1069 t
ORIGIN      
        1 ggcagcctga acatcgacct cgtcgcgtac gcggcccgcg tcccgggcgc gggcgagacg
       61 gtgctggggg accggttcca gatgggcttc ggcggcaagg gcgcgaacca ggccgtcatg
      121 gctgcgcgcc tcggtgcccg cgtctcgatg gttggggcgc tcggtgatga cgtctacgcc
      181 gggatgacgg tcgacaacct gatcgcgcag ggggtcgatg ccgcgcacgt ggcgcgcgtc
      241 gacggctcga gcggcgtcgc gccgatctgg gtcgagcccg atggcacgaa ccggatcatc
      301 gtcgtggccg gcgccaacga tgccgtcgac ccggcgactg cggctgatgc ggttcgagcg
      361 ctcgagggcg ttgccgtggt cgtgggccag ctcgagatcc cgcaacgcgt cacggcagca
      421 gcattcgttg cggcgcgtga ggttggtgcc gtgacgatct tgaatccggc tcccgcggct
      481 gcccttgacc caacactcct cgacggcgcc gattggctca tgcccaacga gaccgagttt
      541 gcgatcctgg ccggcgtcga ttcgttcgac ccggccgacg atgcggccct gcttgcgttc
      601 gcagcgacca tccgacctcg gctcgtggtg acgctcgggt cacgcggtgc cgggctggtg
      661 acgaaagacg gcaccgtcga acgagtaccg gcgattcgcg tggacgcggt cgacacgacc
      721 ggcgccggcg atgcgtttgt cggcgccttc gcttacgggc tggcggccgg gctcccggag
      781 ctcaccgcga tgcgactcgg catcgcatgc gcctccgaca gcgtcacgaa aaccgggacg
      841 cagagctcgt tcgcaactcc ggaaggcgcc ggcgacatct tcgacaaggt tctcggcgcg
      901 gcgtagcgct gagcgcgcgg cgtgcctgag caagctggcg cgggtcggcg tacctcggcc
      961 aactcggcgg cgatgatcac cgggtgatca aatctggcgg ttgcgaggcc atttggtcct
     1021 cgagcgccac cctgacggcg aggttccatc gcaattcgcg ctcaatcgag cgtgacctgg
     1081 tgccgatttg gcgtgcggag catgcgcaag tcggcgaaag ggctgatcaa cccctggaaa
     1141 agatcgccgc gtgatcaccg accgcggttt cgggccgagc cgcccctggg ctgcgtggtc
     1201 ctcgggcttc ggctgcccgg tccttgagct gcgcgcccta ctcctcgaag agctcccagc
     1261 cgccgtcttc gccggggatg aagaacgcgt tgtccgggta gcaccatgcc cagatctcgc
     1321 cgggttgggc ggatcggatc agggcgtggc ccgcctcgtt gtagtgcgcg gtggcgtggc
     1381 ggttcgggct gttgtcgcag cagccggtcc tgccgcagac gaggcactgg cgcaggtgca
     1441 cccaggtctc gcccatctcg acgcatgtcg gacagacgtc gagtgccgcg ggcaggtcgt
     1501 ccatggcggt ggcgaaatgg ggacagggca ggctcacgat ggtcgactca cgggcgcatc
     1561 gtaggcggtc acgacggatc gcgaccaggc gacccggtcg ccgtgcccgc gctccgcatc
     1621 ccgacgagcg agatgaggcc gaggaccccg accgacgccg ccatggcgat cacgacggac
     1681 tcgattccgc cgccgatcgg gatgccggcg gcgatcccgc tcaccatcac cgtggcgagg
     1741 ccgagcagtg cgagcgcacc gatcgccgcg gccctcgacc gatgcagtcc gcgctcgtac
     1801 cggcccggga ccctgctgtc gatcgaccac tgggcgagct cgccgacgat gaggatggcg
     1861 acgccgacca gcccgagctg gaacaggtcc gtctcgttcg tgaggagacc gtgctcgaaa
     1921 tagagcaggg caagcagccc gaggacgacg ctgacgatgc cggcgcgacc gagtgccagc
     1981 gtcatcgcga gcaccagcag gacgaccacg ccgggcgtcg ccgtgatgag cagcacgccc
     2041 cggccgcgtg aatcgagcga cgcgaccacg atggtcgtcg cgaccgcgag tgcgagcgcg
     2101 cccgtgatca ggcggacgcg cggcgggcgt gtcgacgaaa tgctcgaacc tcctccagca
     2161 ccgcatcgag cggctcgttc gacgaccagg ccgcgactgc cacccctgcc cggcgaagac
     2221 gatcacgcag cagctcgcgc tcgagatgcc acagtcgtcg cgcggtggac gcgaacccag
     2281 gagcgggcgg ggcgatgtat tcctccggga gaatctcgat cacggcgaga tcgaagcccc
     2341 gtgccagcag gtcgagaatc gcctcgatcg aacgatcgtc gagcagcggg ctgagcacga
     2401 tgatcagggc cttcgggggc agcgatctcg gcgggatgag gtccacgccc ttccagacga
     2461 acgttgccgc ggcccgggcg ccgaggagca tctcgacgat ccgatagccc tgggcgaggc
     2521 ccatcgaggg caccagccag cgaatggagc cgccgaacgc gatgagaccg acgcggtcgc
     2581 gccgccgaag gtagatgtcc gcgattccgt tcgcgccccg gacggcggtc gcgagactgc
     2641 tgtgcccctc cccatcgccg atgtcggtga acgtgtcgag caggatcacg acgtccgtgc
     2701 tgcgctcggg atggagctcg ttgacataca gctccgagcg ccggctgctc aggcgccagt
     2761 tgatgcgccg aatcctgtcg ccggcggcat acgggcggac gtcggcaaac tcgatgccgt
     2821 cgcctcggcg ccgcgagacc tcgtcgccgg cgtacatctg ggtttcggcg ggtcggatca
     2881 cgcgtcgcag ctcctcgggc ttcgggaaga cacggagcgc gagggctcct tccaggcgcg
     2941 tctcgtaggc gaagaacccg aagcgatcgc gggcccggac gagcacctca ccgacccgat
     3001 aggcgcccca tcggcgggca gcgaccgcga tcgatacgtc gcgttccccg ccggcctcga
     3061 gccggagccc gaccacgcgg accccgtcgg tcgcgacgat gccggcaggg aggacgagcg
     3121 ccagctccag ccagggcacg ggtccctcgg cccgaacgtg caggtcgacg ttgacctgct
     3181 gcccttcgag caggcgactg gacgcgatcg tcgcctggat cgagatctcg ggttcctcga
     3241 cgaggaccag cgccaggagc gcagcgatca gcagcggcgc aacgaggatg accggctcca
     3301 cgcggccgaa tgccaccgcg gcgatcaggc cggcggcgcc aagggcgagg tacgcccgga
     3361 gcctcggcga cgggcgccgg gtcacgacgt gacgggcgcc ggttcctggg ccgacggcgc
     3421 cggcaccgcg tccaggcact cctggaccac ctgctccgcg cgcacctgct ggacccacag
     3481 ctcgggacgc aggatcaggc gatgtgcgag cgcgggtacg gccactgcct tcacgtcgtc
     3541 ggggacgacg tagtcgcgcc cgtccatcac ggcgcgagcg cgactcagct tgagcagcgc
     3601 aagggtgcct ctcgggctgg caccgacctg caggcgcgcg ctggcccgcg tcgcgcccac
     3661 gagcgcgact acgtaccgaa tgatgctttc gcccacgaac acgtcttcga cgcctgcctg
     3721 catggcggac accgtcgcgg cgtcgaccac gcccttgagc tgcacctcgt ccgccttgcg
     3781 ctcgatgcgg gcccgcagga tcgtcgcctc ctccgagcct gacggatagc cgacgcggag
     3841 ccgcaggagg aagcggtcca gctgcgcctc ggggagcggg tacgtgccct cgtactcgat
     3901 cgggttctgg gtcgcgatca ccaggaacgg tcgcggcagc tgtcgggtcg tgccctcggt
     3961 cgtgacctgg cgctcctgca tggactcgag cagcgacgac tgtgtcttgg cgggggcgcg
     4021 gttgatctcg tcggcgagga gcaggttcgc gaagacggga ccctcacgga acacgaactc
     4081 ggcggtcttc tggttgtaga tcgacgctcc cgtcacgtcc gagggcatca ggtccggggt
     4141 gaactggatt cggcggaact ggaggccggt gacctgcgcg aacgagcgcg ccatgagggt
     4201 cttcgcaagg ccggggaggt cgtcgatcag cacgtggccg tcgctcagga cggcgagcag
     4261 gaagagttcc aggacgtcgc gcttgccgac gaccgcacgt tcgacttcgt cgaggatggc
     4321 ccggcctcgg gccgcgatgt cgggaattgg cattcgatcg gtcacgtcag agttgctcca
     4381 gctcattgat cgccaggttg acctcgtcga gggccgcgcc cggtccgtcg aatgccgcgc
     4441 cggcgccgcc ggtcgatatg aggcgcgaca accgatcacc gaggaagcgc tgcgctgctt
     4501 cgggcgccgc gtcgaggtcg acggcgtgac tccgttggag gcgccaccgg accagggctc
     4561 gaagtcgcgg cttcagcatc gtctcgagcc caaaggcacc cccgctcgac atgcgcagcg
     4621 cggtctcaac ggaacggagg ccgctgatct cgggccgctg cccggccgga ggcgccaggt
     4681 cgagctcgaa gcgctccgac gttgacgcgg tgatcgcatc gctccgaccg aggacgcgcc
     4741 acgccgcgac gatgccgatc acgaggatcg ccacgcggcc gacggacccc tgggcgtcgg
     4801 gtggcgcgag gacgaaggcg accagggcga tcgcaaccat cgcggcgaac gcgatcggct
     4861 cccgcccgac ggcgggcgag cgaccggggc gggccccatc agggctgggc ggcgcgattc
     4921 gcttgctccg gtcgaactcg taggtcatgc gctcgcgctc gcagccgtcc ggatccgctc
     4981 caacgcggtg atcgcgccgc tgcgcatcgt ctcgtcaacg gcgtgatcgc tgaacttcgc
     5041 gtgttggaac aggagcgtga gcgtgcggac ttcgtcgacc gcgtgggtag aggctgccag
     5101 cacgcgacgg aggtattcga gcggcgcctc tgactcctca cgtgggtatc cctcgtgggc
     5161 cagggatcgc tccatcgccg cgtacgccgc aatcacggct cggcgcgggt cgggctcgcg
     5221 gcggatcgcc tcgaggctca cctcgacggc ggccaccgtg ctcgccgccc gccctcgatc
     5281 gatccgcttg ccgcgcgtgt cggtgaggcg ccatcgaatg aggagcccca cgacgaccag
     5341 gaagatcacg atgacgatga ccatggcgat gcccagcgcc ggggagtcag cccgactggg
     5401 gtccagtcga ccgacgacgt cctgcagcag cccgccaatg ccggtgcctt cgtccgccct
     5461 gcgctgcagc agctcgatga tgaagttgag gatgatcgcg acctggaaca cgatcagcgg
     5521 aatcacgacg actgcgccca gcaggttcgc ccaccaaggc gccggcgggc gcttcggttg
     5581 ggctgccgcg gccgccatcc gcatctgccg ggcgcggccg tgcgccacgg cgatgatcgg
     5641 cagcacggcg atgccgatcg cgaggaacac gtagccaatg acttcgagga ccgtgattcc
     5701 ggactcgagc ccgatcggtc cggtactggt gccggtcgcc tgcgaccgac tcgtgagcgc
     5761 aacgagcgcg atgaggccgg cggcagcggg cagtgcgacc cagccgggaa tgcgcgtcgc
     5821 gccgctcggt ggcgcttcgt cctccgtatc agccacgtgt cgttggctgc tcgggcactg
     5881 ggtgatcctt tccgaccggg ccgatccctg ggttcggccc gcctccaggc gggaggatag
     5941 caaggcgcag cttcggcacc ggcattcgaa gcgtcagtcg tcgaagctga cagaggtcct
     6001 cgacatgtcg agtggctccg aaagacggat caggaggctg cgcccgatcc cgatcgcatc
     6061 gcagccgccc ggcttcctgt tggtggtgat catgaagtcg agattcggcg gtgcgctgac
     6121 ggtgattgca acgctctcgt cgcaggcgcc cccggtccag aacacgacca gggcgttcgg
     6181 attcctgtcg accgtgccga tccctgcagg gatcggacgc tctgggacgg gtggcccatc
     6241 gtcgacgctc acgatgatgc cgctctggtc gtgcaccacg accggcagcg cggcgatgtc
     6301 gccaacggcc ggaaacgaga aatcgaaggt ccgggagaac cagctgcatc cgacgagcac
     6361 caccgcggcg agcagggcca ccgacgagag gcgcaacgcg gacatcaagg ggtgacggtg
     6421 aagtcgagct ccagcgtgcc ctcgtgatcg tcgagcgtgc agacgatggt gatcgggtac
     6481 gttcctgcct tcgtattgct cccgaccttc cacgaccaga tcaccttgcc gccgctcgtc
     6541 gccttcttcg ggtcgagccc ggccgcctgg ctcggcccgc tgtcgtacag gacctcgatc
     6601 gtgcacctgg ccgaaggttc ggtccggatc gtgaccgacg ccgtcttggt cgtcgcgacc
     6661 ttggccgtga aggcttcctt cgtcacgtcc atcgggagcg gtgtgggctt cgccgtcgga
     6721 gtgggcttgg gcgtcggagt cggcttcggc gttgcaggca ccgatgtggc caggagcgca
     6781 tccggactcg tcgcggcagg cccgcccgag ccgcacgcgg ccgtgatgaa tgcgatgaac
     6841 aacgccgcaa gcgtcatgcc gcctgaccgg cgtgctctgt ccagaccgtg catgtggtgg
     6901 ctcctcatga tggcgccgac gcagggatga cccgatccag gcagtggtgg atggcggaga
     6961 gggtgggatt cgaacccacg ggacttacgt ccgcctgttt tcaagacagg accgttcaac
     7021 cgctccggca cctctccacg cgggaggata gcaacggcct agccggcggc ggcgatccga
     7081 tccatgccga ggtacggccg caggacgtcg gggacgacga tcgagccg
//
LOCUS       DATBSW010000188         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_828268, whole genome shotgun sequence.
ACCESSION   DATBSW010000188 DATBSW010000000
VERSION     DATBSW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828268"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VIF63_05250"
     CDS             complement(<1..327)
                     /locus_tag="VIF63_05250"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238822.1"
                     /translation="MSFAAPTERPLAVPAGRLRQTFIALRERDFRIFFIGQLISVTGT
                     WMQTVAQGWLVLTLTGSPFVLGVAAAARSIPVLILVVPAGIAADRFDRRKIVVTTSIV
                     STVASGL"
     gene            complement(371..2674)
                     /locus_tag="VIF63_05255"
     CDS             complement(371..2674)
                     /locus_tag="VIF63_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8238823.1"
                     /translation="MSAIADPPAGLPAGSTAELSLPIEGMTCASCVNRIERFLKKTPG
                     VEEAVVNLATEVATIRYLPDQAGRAELVAAVEAAGYDVRPAPTVAAGDRAGELVRAAD
                     IDAAQRASEARALLVRAAASIGVGVGIMALMLLATGVAMEDLNRIALLPATFIQVWAG
                     GRFYRAAWRAARHGATNMDTLIAVGTSAAWGYSVAVTLWPAIATSAGLEPAAYFDSST
                     IIVGFVLLGRWLEARAKGQAGSAIRKLLALEPPIARRIAGATEVDVPVADVQPGDLLR
                     VRPGDRVPVDGLVMSGSSAIDQSMLTGESMPVEVGEGATVIGGTLNGTGSFVMRATRV
                     GRDTVLANIVDLVQRAQGSKAPIQRLADRVSEVFVPIVLVAAALTFATWLAIGPEPRL
                     TLALTAFVSVLIIACPCAMGLATPTAIMVGTGRGAEAGILIRGGEALELAHRVNTVVF
                     DKTGTLTEGAPAVAGVTALRGFEASEVLDLAASLEVGSEHPIGDAIVAHARGTGVGGR
                     EVIGFRSIAGGGVEGTVQLVGGKCDVLIGSARLVGDRGIDLAPIAEQLGPHGSAGTSV
                     IVAIDGRAAGVITVRDRTKPAAKAAIAELRAADVDVWLLSGDSRNVAEALAEDVGIRA
                     DRVLAEVAPDGKAEAIQRLRADGRVVAMVGDGVNDAPALASADVGIAIGTGADVAIEA
                     ADVTLVGGDPRDVAAAIRLSRATMRVVRQNLAWAFAYNVILIPVAMGALFPFTGLLLN
                     PALAAGAMALSSVSVVANSLRLRNVRL"
     gene            2734..3315
                     /locus_tag="VIF63_05260"
     CDS             2734..3315
                     /locus_tag="VIF63_05260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY8238824.1"
                     /translation="MEPTVSASIDAPWGPIRLAATNRGLAALDQLVSLDAFDERLERR
                     FGTPPIDLAAAPSSAAAAATHLRAALAAVRAFLDGNLDALDALQLDLADRTGWDRLVL
                     GAVRGIPVGTTASYGEVARMIGRAGAARAVGGSVGRNPMGLVIPCHRVIAGDGTLGGY
                     GGGWWGGRQAGLELKRELLGREGVHIQPPRDGP"
     gene            3349..>3758
                     /locus_tag="VIF63_05265"
     CDS             3349..>3758
                     /locus_tag="VIF63_05265"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238825.1"
                     /translation="MFDIFKKREFTLLWFAQLVSTIGSSLTDLAAGILVFQLTGSALN
                     VGLVLMVTAIPTLFVGLFAGVFVDRFNRKWILLASDILRGILVLMIPLAFDAFGTDTG
                     MLAMYGLLFLAATVRTFFDPAWESVLPEIASEEEL"
BASE COUNT          540 a         1377 c         1308 g          533 t
ORIGIN      
        1 caggccggac gcaaccgtcg agacgatgct cgtcgtgacg acgatcttcc gccggtcgaa
       61 ccgatccgcg gcgatccccg cgggaacgac gaggatcagc acggggatgg agcgcgcggc
      121 ggcggcgacg ccgagcacga atggcgatcc ggtgagggtg aggacgagcc agccctgggc
      181 gaccgtctgc atccacgtcc cggtgacgga gatgagctgg ccgatgaaga agatccggaa
      241 gtcccgctcg cgcagggcga tgaaggtctg gcgaaggcgg cctgccggca cggccagcgg
      301 ccgttccgtc ggcgcggcga atgacacgct gaaagtgtag gagcccgggc ccgcgcggcc
      361 ccgggacggg ctacaggcgg acgttgcgca ggcgcagcga gttggcgacc accgagaccg
      421 aggacagggc catcgcgccg gccgcgaggg ccggattcag cagcagcccg gtgaatggga
      481 agagcgcacc catggcgacc gggatgagga tcacgttgta ggcgaaggcc caggcgaggt
      541 tctggcggac cacgcgcatg gtcgcgcggg agagacggat cgccgcggcg acgtcgcgcg
      601 ggtcgccgcc gaccagggtt acgtccgcgg cttcgattgc cacgtctgct cccgtcccga
      661 tcgcgatccc gacgtcagcg gaggcgaggg ccggtgcgtc gttgacgccg tcgccgacca
      721 tcgccacgac ccgcccgtct gcccggagtc gctggatcgc ctcggccttg ccgtccgggg
      781 cgacctccgc gaggactcga tccgctcgaa tgcccacgtc ctcggcgagc gcctcggcga
      841 cgttccggct gtcgccgctg agcagccaga catcgacatc ggctgcacgg agctcggcaa
      901 tcgcggcctt ggcggcgggc ttcgtccggt ctcgcaccgt gatcacgcct gccgctcgcc
      961 cgtcgatcgc gacgatgacg cttgtgcccg ccgatccatg tggcccgagc tgctcggcga
     1021 tcggcgcgag gtcgatgccc cgatcgccga ccaggcgcgc actgccaatg agaacgtcgc
     1081 acttcccgcc gaccagctgg accgtcccct cgacgccgcc cccggcgatc gagcggaaac
     1141 cgatgacctc gcgaccaccg actcccgtcc ctcgggcgtg cgcgacgatc gcgtcaccga
     1201 tcgggtgctc gctgcccacc tccagggacg ccgcgaggtc gagcacctcg ctggcctcga
     1261 agccgcgcag ggccgtgacc ccggcaaccg ccggggcgcc ctcggtcaac gtgccggtct
     1321 tgtcgaacac cacggtgttg acgcgatgcg cgagctccag cgcctcgccg ccgcggatca
     1381 ggatgcccgc ctccgcgccg cgcccggtgc cgaccatgat cgcggtcggc gtcgcgaggc
     1441 ccatcgcaca cgggcaggcg atgatcagca ccgagacgaa cgcggtcagc gcgagggtga
     1501 gccgcggctc gggcccgatc gcgagccatg tcgcgaaggt cagcgctgcc gcgacgagga
     1561 cgatcggcac gaagacctcg ctgacgcggt cggcgaggcg ctggatcggc gccttgctgc
     1621 cctgggcacg ctggacgagg tcgacgatgt tcgccagcac cgtgtcgcga ccgacccgcg
     1681 tcgcgcgcat cacgaacgag cccgtgccgt tgagcgtgcc gccgatcacg gtcgcgccct
     1741 ctccgacctc gaccggcatt gattcgccgg tcagcatcga ctggtcgatc gccgacgagc
     1801 cgctcatgac gaggccatcg accggcacgc ggtcgccggg ccgcacccgc agcaggtcgc
     1861 ccggctggac gtccgcgaca ggcacgtcga cttctgtcgc gccggcgatc cgtcgcgcga
     1921 tcggcggctc gagcgcgagc agcttccgaa tcgcgctccc ggcctggccc ttcgcgcggg
     1981 cctcgagcca ccgcccgagc aggacgaagc cgacgatgat cgtcgacgag tcgaagtacg
     2041 cggcgggttc caggccggcc gacgtcgcga ttgccggcca cagggtcacg gcaaccgaat
     2101 agccccatgc cgccgaggtc ccgactgcga tcagcgtgtc catgttcgtt gcgccgtggc
     2161 gggccgcgcg ccacgcggcg cgatagaacc ggccgccggc ccagacctgg atgaacgttg
     2221 ccggcaggag cgcgatccgg ttcaggtcct ccatcgcaac gccggtcgcg aggagcatca
     2281 gggccatgat cccgaccccg acaccgatcg acgcggcggc gcggacgagg agagcccgcg
     2341 cctcgctcgc gcgctgcgcg gcatcgatgt cggccgcccg aacgagctcc cctgcgcgat
     2401 caccggccgc gaccgtcggt gcgggccgca cgtcgtagcc cgcggcctcg accgcggcaa
     2461 ccagctctgc gcgcccggcc tggtcgggca ggtagcggat cgtcgccacc tcggtggcga
     2521 gattcaccac ggcttcctcg acgcccggcg tcttcttgag gaagcgctcg atccggttca
     2581 cgcacgacgc gcaggtcatg ccctcgatcg gaagcgagag ctcggcggtg gatccggcgg
     2641 gcaatccggc gggcggatca gcgatggcgc tcatactccc ccggagtata cccttatgcc
     2701 gcccgacggc tgcgaatcta tcctcgactg accttggaac cgaccgtcag cgcctcgatc
     2761 gacgcgccct ggggaccgat ccgcctcgcc gccacgaacc gcggtctcgc ggccctcgac
     2821 cagctcgtct ccctcgacgc gttcgacgag cgcctcgagc ggcgcttcgg caccccgccg
     2881 atcgaccttg cggcggctcc gtcatccgcg gcggccgcag cgacgcacct gcgcgccgcc
     2941 cttgcggccg tccgtgcctt cctcgacggc aacctcgacg cgctcgacgc cctccagctc
     3001 gacctcgcgg accggaccgg ctgggaccgc ctcgtccttg gcgccgttcg cggcattccc
     3061 gtcggtacga cggcgagcta cggcgaggtg gcacggatga tcggccgcgc cggggcggcg
     3121 cgcgctgtcg gcggctcggt cggccggaac ccgatgggat tggtcatccc atgccatcgg
     3181 gtgatcgcgg gcgacggcac gctcggcggc tacggcgggg gctggtgggg cggccggcag
     3241 gccggcctgg agctgaaacg cgagctgctc gggcgcgagg gcgtgcacat ccagccaccg
     3301 cgcgacggcc cctgaatccg gcggttctgc gccggtagac tcgccgggat gttcgacatc
     3361 ttcaagaagc gcgagttcac gctgctgtgg ttcgcgcagc tcgtctcgac gatcggctcg
     3421 tcgctgacgg atctcgcggc cggcatcctc gtcttccagc tgaccggatc ggcgctgaac
     3481 gtgggcctcg tgctcatggt caccgccatc ccgacgctct tcgtcggcct gttcgcgggt
     3541 gtctttgtcg accggttcaa ccgcaagtgg atcctgctgg cctcggacat cctccgcggc
     3601 atcctcgtcc tgatgatccc gctcgcgttc gatgcgttcg ggacggacac cggcatgctg
     3661 gccatgtacg ggctcctgtt cctcgccgcc acggtccgga ccttcttcga cccggcctgg
     3721 gagagcgtcc tgcccgagat cgcctcggag gaggagct
//
LOCUS       DATBSW010000189         6699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_828345, whole genome shotgun sequence.
ACCESSION   DATBSW010000189 DATBSW010000000
VERSION     DATBSW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828345"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VIF63_05270"
     CDS             <1..316
                     /locus_tag="VIF63_05270"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEY8238826.1"
                     /translation="DHRSDASYPIAKLPEFIRLHETVAGLVAPSKVVAIALNTMLYPD
                     DDDARAVIAEIAAETRLPTDDPVRFGAARLWPAVRAAVDALPWVARGLPVVPSAASRA
                     VS"
     gene            313..1359
                     /locus_tag="VIF63_05275"
     CDS             313..1359
                     /locus_tag="VIF63_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEY8238827.1"
                     /translation="MTLALSHEVLRLGLRDPFRIARSDHDAGLAVTTVVVELRDDRFP
                     GVVGLGEGYPDRFYGENVDTQAAVFPLLLSAIEGFDPTPDGVRAASNRLAAAIRWNGG
                     AKCAIDTAMHDLAGKVARVPVYQLLGLSTDIPPTDFTLGLDEPAVVAERARRAGAFPA
                     LKIKCGGPADLATLEAVRSVYDKPIRVDANTAWTPEIARQLLPALVDLGVELIEQPFP
                     ANRFDWLRDLQAGSPLPIVADESCVTIDDLEPLVGVTAGVNVKLAKCGGPGQAAQMLA
                     RAHELGFRTFLGCMEETSIAIAASAAVASLADWADLDGNLLLADEPFQGLVLGEDHRW
                     QLSDRPGLGVSRRS"
     gene            1593..1895
                     /locus_tag="VIF63_05280"
     CDS             1593..1895
                     /locus_tag="VIF63_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238828.1"
                     /translation="MVARSATPHAITADAVEFIRYCYARRRVGWPELYDEMCAVASRG
                     LFRGWGPDELAAEGIGFGLFDMPRLASTVTRIVAEDRARVKPAVVPKATATATAVA"
     gene            complement(1953..2180)
                     /locus_tag="VIF63_05285"
     CDS             complement(1953..2180)
                     /locus_tag="VIF63_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238829.1"
                     /translation="MKQMYHPSDLAAMDPLVLMKNLDHVRMTSRRLSYILQQQVHLYT
                     PEANQLRDQIDGYVEAERQIEGEMARRQIRA"
     gene            complement(2177..2683)
                     /locus_tag="VIF63_05290"
     CDS             complement(2177..2683)
                     /locus_tag="VIF63_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY8238830.1"
                     /translation="MIVRAALPTSLERVRRRSVWNAPDRVPAHLTMLYPFVAPDRLHS
                     DVRIALAAVAARVAPFEYRLVGAAVWPQTVYVAVDPVEAFLALHRELQAAFPAFPIYG
                     PDPGFDFVPHVTIAEGDAFDEAAILADPGWRLLPLARRAVAIEVITQPEGGRWRTIWR
                     LPLGRMPA"
     gene            complement(2716..4059)
                     /locus_tag="VIF63_05295"
     CDS             complement(2716..4059)
                     /locus_tag="VIF63_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY8238831.1"
                     /translation="MTAGQTSGSSQQLELGLGAPRLAIEPEWKAQPRLWGHSLHPMCS
                     YLASFPATLAHAFIARYSRPGDVVLDPFSGRGTTPLQATAEGRIGVGNDLNPFAHLLT
                     AAKVEPPTVAEARTRTTALRLAWASSSRAWLEQAAAIQAAPATGGPTPVPDEVALAFH
                     PRTFAQVLFLRATLDQRTPVDRFLVAAIAGMLHGKTPSYLSTLMPNTFSMAPRYVREF
                     AARTGFESPERDAFDRLDAKLTRLFRDAPPRTAGLALLGDARDAGTRASAELRARRLP
                     DRARLVITSPPYLRVVKYGYYNWLRTWFLGFDSKAIDASLDDAHHRAPYLEFLRTVLH
                     DLRPAMTDDGIAVLVIGDVATDRGRAHPEGEGLAERVWESAAAPEGFHLAGIADDDVH
                     AHRKMTRLWGREAGRATQVDRILVLGASEAGRRRAISSARNPISWDWPPRGLRSI"
     gene            complement(4152..5357)
                     /locus_tag="VIF63_05300"
     CDS             complement(4152..5357)
                     /locus_tag="VIF63_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238832.1"
                     /translation="MTSSKAPATPAAPGDRLLLTQVPPAADLGWARITWRKLAADDPL
                     ALVRSIVAWRGGYIAVGAPVPTGASSTTPVWTSTDGGAWQPVGPDQFGSSALLLGVGE
                     TAGGLVALTLEGGVNACGGSTYELDCWTPALPFQAWASSDGTTWTSHPGPDIELNSIC
                     EECGVEPPTFGAGQPGLVVIGAETAVSSDGVSWEVLESSGFPAGDDARSGTMVAFGSG
                     FVAVGERNIKIGDEAGTQAIGYRSTDGRSWAATRMVIARPAEHGTGASSLVAGSAGLI
                     AKGSTAGDPGAELWWSSPDGSTWTELLDYPPLGTWHGQAQGSGASENGTLVGNGDRML
                     AYRSDSGEAAWTSFDGVTWEQLAVDGEAPIWGDVQHPDLILLSVGVLGFGHDGAVWIG
                     EPEAELELE"
     gene            5646..5721
                     /locus_tag="VIF63_05305"
     tRNA            5646..5721
                     /locus_tag="VIF63_05305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5679..5681,aa:Arg,seq:tct)
     gene            5782..6174
                     /locus_tag="VIF63_05310"
     CDS             5782..6174
                     /locus_tag="VIF63_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8238833.1"
                     /translation="MSAAGPVVVHHPEGTPGQEVPGMERRQQFEREGRWASWIRVESG
                     NVTPWHHHAANDTYVYVSRGSASIEFGPGGAETVVAGAGDFFHVPPGTIHRETIGTDA
                     DFDGFVLRIGTAPERVDVDGPDAAGGSA"
     gene            6491..>6699
                     /locus_tag="VIF63_05315"
     CDS             6491..>6699
                     /locus_tag="VIF63_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238834.1"
                     /translation="MPIDDVFARSKAPVPEGCGPKRTDVAACILCHGNGDDLDLGGVR
                     LDPQLPGFCRNPACQLDITIAQRSVV"
BASE COUNT          975 a         2328 c         2385 g         1011 t
ORIGIN      
        1 cgaccatcgg tccgacgcgt cctacccgat cgccaagctg cccgagttca tccgactgca
       61 cgagacggtg gcggggctcg tcgctccctc gaaggtcgtc gcgatcgcgc tgaacacgat
      121 gctctacccg gacgacgacg acgcgcgagc cgtcatcgcc gagatcgccg ccgagacgcg
      181 actgccgacc gacgatccgg tccgcttcgg tgcggcccgc ctgtggccgg ccgtgcgcgc
      241 ggccgtcgac gccctgccct gggtcgcgcg ggggttgccc gtggtgccgt cggctgcgag
      301 ccgcgccgtc tcgtgacgct cgccctcagc cacgaggtcc tccgcctggg cctgcgcgac
      361 ccgttccgga tcgcgcgctc ggaccatgac gcggggctcg cggtcacgac cgtcgtggtc
      421 gagctccgtg acgatcgatt tcccggggtg gtcgggctcg gcgagggcta cccggaccgg
      481 ttctacggcg agaacgttga cacgcaggcc gccgtgtttc cgctcctgct ctccgccatc
      541 gaaggcttcg atccgacgcc cgacggcgtc cgcgccgcca gcaatcggct cgctgccgcg
      601 atccgctgga acggcggggc gaagtgcgcg atcgacaccg cgatgcacga tctggccggg
      661 aaggtcgccc gtgtgccggt gtaccagctg ctgggcctgt cgacagacat cccgccgacc
      721 gacttcaccc tcgggctcga tgagcctgcc gtcgtcgccg agcgcgcacg ccgggccggc
      781 gcgttccccg cgctcaagat caagtgcggc gggccggccg acctcgcgac gctcgaggcg
      841 gtcagaagtg tctacgacaa gccgatccgg gtcgacgcca acacggcctg gacgcccgag
      901 atcgcgcgcc agctgctgcc cgcgctcgtc gaccttggcg tcgagctgat cgagcagccg
      961 ttccccgcga accggttcga ctggctgcgc gacctccagg ccggctcacc gctgccgatc
     1021 gtggccgacg agtcgtgcgt cacgatcgac gacctcgagc cgctcgtcgg cgtgaccgcc
     1081 ggcgtcaacg tgaagctcgc gaagtgcggc gggcccgggc aagcggcgca gatgctggcg
     1141 cgcgcgcacg agctgggatt ccggacgttc ctcggctgca tggaggagac gtcaatcgcg
     1201 atcgcggcgt ccgcggccgt cgcgtcactg gccgattggg ccgatctcga cggcaacctg
     1261 ctcctcgccg acgagccgtt ccagggtctc gtcctcggcg aggaccatcg ctggcagctc
     1321 tcggaccgcc ccgggctggg cgtcagccgg cgtagctgag gggtccgcac cgggtccgga
     1381 ttcggtggtg tggataagtt ggtggacgaa aaggtggaga accccccttc cggcaaaagt
     1441 aggccccgcc gtatccttgc catacacctg aggaccctcc cggagagcgg gcccgccggt
     1501 cacctgatat cccaaatcgg gccacggccc atcacgccgt tggacccggt cagcaccgct
     1561 gtgcccctgc gagggacgag gaggtcgtat cgatggtcgc gcgctccgcg accccgcacg
     1621 ccatcacggc tgacgccgtc gagttcatcc gctactgcta cgcccgtcgc cgggttggct
     1681 ggccggagct ctacgacgag atgtgcgccg tggcgagccg gggactcttt cggggctggg
     1741 gccccgacga gctcgctgcg gagggcatcg gcttcggcct gttcgacatg ccgcgcctgg
     1801 cctcgacggt cacgcggatc gtcgccgagg accgggctcg ggtgaagccc gccgtcgttc
     1861 ccaaggcaac ggcaaccgcc actgcggtgg cgtagtccga cggaacgacg cccggaggcc
     1921 accgcagtgc ggcggccgtc gggcaagcgg cctcaggcgc ggatctggcg cctcgccatc
     1981 tcaccctcga tctggcgctc ggcctcgacg tacccgtcga tctggtcgcg cagctggttc
     2041 gcctcgggcg tgtacaggtg gacctgctgc tggaggatgt agctgagccg gcgactcgtc
     2101 atccggacgt ggtcgaggtt cttcatcagc acgagcgggt ccatcgccgc gaggtcggac
     2161 gggtggtaca tctgcttcac gccggcattc taccgagcgg gagccgccag atcgtgcgcc
     2221 accgaccacc ctcgggctgc gtgatcacct cgatcgccac agcgcgtcgc gccagtggca
     2281 gcaagcgcca gcccgggtcg gccaggatcg ccgcttcgtc gaacgcgtca ccctcggcga
     2341 tcgtcacgtg gggcacgaag tcgaagccgg ggtcggggcc gtagatcggg aacgccggga
     2401 acgccgcctg cagctcgcgg tgcagcgcga ggaacgcttc gaccggatcg accgcgacgt
     2461 agaccgtctg tggccagacc gccgcgccga cgaggcgata ctcgaacggc gctacccgtg
     2521 ccgcgacggc cgccagcgcg atgcggacgt ccgaatggag tcgatcgggc gcgacgaacg
     2581 ggtagagcat ggtcaggtgg gccggcaccc gatcgggcgc gttccagacg gatcggcgcc
     2641 gcacgcgctc gagggaggtc ggcagcgcgg cccggacgat caccgcagac cgctccgatg
     2701 gcgacccgag gtcgctcaga tcgatcgcag gccgcgcggc ggccagtccc acgagatcgg
     2761 gtttcgtgcc gaggagatgg cgcgtcgccg cccggcctcc gacgcgccga ggacgaggat
     2821 tcggtccacc tgcgttgcgc ggcccgcctc gcggccccag agccgggtca tcttgcgatg
     2881 ggcgtggacg tcgtcatcgg cgatgcccgc gaggtggaag ccctccggcg cggccgctga
     2941 ttcccagaca cgctcggcga ggccctcgcc ctcgggatgg gcgcggccgc ggtcggtcgc
     3001 cacgtcgccg atcaccagca ccgcgatccc atcgtcggtc atggccgggc gcaggtcgtg
     3061 gaggaccgtg cgcaggaact cgaggtaggg cgcgcgatgg tgggcgtcgt cgagcgaggc
     3121 gtcgatcgcc ttcgaatcga agccgaggaa ccaggtgcgg agccagttgt agtagccgta
     3181 cttgaccacg cgcaggtacg gcggcgaggt gatgacgagt cgggcgcgat cggggagcct
     3241 tcttgcccgc agctcggcgc tggctcgggt gccggcgtcg cgggcgtcgc cgaggagcgc
     3301 gagcccggcg gtgcgcggcg gggcgtcccg gaagagccgg gtcagcttgg cgtccagtcg
     3361 gtcgaacgcg tcgcgctccg gcgactcgaa gccggtccgg gcggcgaact cgcggacata
     3421 gcgcggggcc atgctgaacg tgttgggcat gagcgtggac aggtacgacg gcgtcttgcc
     3481 gtgcagcatg cccgcgatcg ccgcgacgag gaagcggtcc acgggcgtcc gctggtcgag
     3541 cgtcgcgcgg aggaacagca cctgggcgaa cgtgcgcggg tggaacgcga gcgcgacctc
     3601 gtcggggact ggcgtcggac cgccggtcgc cggcgcagcc tggatcgccg cggcctgctc
     3661 gagccatgcc cgcgacgagc tcgcccaggc gagccggagg gcggtcgtgc gggtgcgtgc
     3721 ctcggcgacg gtcggcggct cgaccttggc cgccgtgagc aggtgggcga acgggttcag
     3781 gtcgttgccg acgccgatgc ggccctcggc ggtggcctgc agtggggtcg tgccgcgccc
     3841 ggagaacggg tccaggacga cgtcacccgg ccggctgtag cgggcgatga acgcatgggc
     3901 gagggtcgcg gggaagctgg caaggtagga gcacatcggg tggagcgaat ggccccacag
     3961 ccgtggctgg gccttccact cgggctcgat cgcgaggcgc ggggcgccga ggccgagctc
     4021 gagctgctgc gaactgccgc tggtttggcc cgcggtcatc cggcgatctg cactccgatt
     4081 tcggcccctg agggcccgag ggcggcggag catagcacgg cggattcgtg gttccgggtg
     4141 cgtcgtcggc cctattcgag ctcgagctcg gcctccggct caccgatcca cacggcgccg
     4201 tcatgcccaa atccgaggac gcccaccgag agcaagatca gatcggggtg ctgcacgtcc
     4261 ccccagatcg gcgcttcgcc atcgaccgcc agttgctccc aggtcacgcc gtcgaacgat
     4321 gtccacgccg cctcaccgga gtcgctgcgg taggcgagca ttcggtcccc attcccgacg
     4381 agcgtgccgt tctcgctcgc gccgctgcct tgggcttggc cgtgccaggt cccgagcggt
     4441 gggtagtcga gcaattccgt ccacgtcgac ccatccgggg atgaccacca cagttccgct
     4501 cctggatcgc cggcggtgct tcccttggcg atgaggcccg ccgagccagc cacgaggctg
     4561 cttgcgccag tgccatgctc ggccggtctc gcaatcacca tccgggttgc tgcccagctg
     4621 cgtccgtccg tggagcggta gccgatcgcc tgggttccag cttcgtcgcc gattttgatg
     4681 ttgcgttcgc cgaccgctac gaaacccgaa ccgaaggcga ccatggttcc ggagcgtgcg
     4741 tcatcaccgg ccgggaaacc gctcgattcc aggacctccc aactcacgcc atcggatgag
     4801 actgccgttt ctgcaccgat cacgacaagg ccgggctgcc cggcaccgaa cgtgggcggc
     4861 tcgaccccgc attcctcgca gatcgagttc agctcgatgt cgggtccggg atgtgacgtc
     4921 caggtcgtgc cgtccgaaga agcccaggcc tggaacggca gagccggtgt ccagcaatcc
     4981 agctcgtagg tcgacccacc acaggcgttg actccaccct cgagcgtcag cgcgacgagt
     5041 ccacccgccg tctcaccgac gcccaggagg agggcgctgg acccgaactg gtcgggcccc
     5101 acgggttgcc acgccccgcc gtccgtcgag gtccagaccg gggtcgtcga cgacgcgcct
     5161 gtggggaccg gagcgccgac ggcgatgtag ccaccgcgcc aggcgacgat cgagcgcacc
     5221 agcgcgagcg gatcgtcggc cgcgagcttg cgccaggtga tccgcgccca gcccaggtcg
     5281 gcggccggcg ggacctgggt cagcagcagt cgatcgccgg gtgcagccgg tgtggcgggc
     5341 gccttggacg aagtcacgtt cgttggcttc gcggttggtg cattgccgct cgcgctgggg
     5401 actggcgtcg cactcgggac agtcgcggtc gcgcacgccg cgagcatgag tccgatgaca
     5461 cccgcggtga ttctgcctgc gcgtgcgacc tgcatctcga cctcggcgct ccatgaggga
     5521 cggtacaccc ggcctcgccc ccagtacgac gccggccctg cccgagttgt atcgacctcg
     5581 ccgtcttggg gacctcggtc cgggctggcc gctccgcgga tggtgcgtag actggcgccg
     5641 cgcacgtccc cgtagctcag tggacagagc ggctgccttc taagcagcgg gtcgcaggtt
     5701 caaatcctgc cggggacgcc aaacctctta cgaaaggccc gagcggtgcc atgctggcca
     5761 atcgcaagac atgaggcaga gatgagcgca gccggaccag tcgtcgtcca ccatcccgag
     5821 gggacgccgg ggcaggaggt gcccgggatg gaacgacgtc agcagttcga gcgcgagggc
     5881 cgatgggcca gctggatccg agtcgaatcg ggcaacgtca ctccttggca tcaccacgcc
     5941 gcaaacgaca cctacgtgta cgtcagccgt gggtcggcgt cgatcgaatt cgggccgggc
     6001 ggcgctgaga ccgtcgtggc cggcgcgggc gacttcttcc acgttccacc tgggacgatc
     6061 caccgcgaga cgatcggcac tgatgccgac ttcgacggat ttgtgctgcg catcggcacc
     6121 gcgccggagc gcgtcgatgt tgatggaccg gacgcggcgg gaggctcggc ataaccgaac
     6181 gacgccatcg ttcggttcat tagccggggc ggcggcgacc caatcacgtc cgggacgcca
     6241 aacctcttgc ccgttactcg tcgtcctcga aatccggcca ggctgcgatg tcgacaccgt
     6301 ggttctcggg cgaggcgagg gtccaccaat gcggcgcgtg cgagccgtcg gccaggcgtc
     6361 cgcccgcttc gagtgccgcg gcgactcgtg cttcggcctg ctcggccgga acgctgacgt
     6421 cgatatggct ccggccccgt cccggcctcg gcgggcggag gctctggaac gatacgtgcg
     6481 ggttccgtcg gtgccgatcg acgacgtctt cgcgcggtcc aaggctcctg tacccgaggg
     6541 ctgcggccca aaacggaccg acgtcgctgc ctgcatcctg tgccacggca atggcgacga
     6601 cctggacctg ggaggggtcc ggctcgatcc gcaacttccg ggcttctgcc gaaatccggc
     6661 gtgccagctc gatatcacga tcgctcagcg ctccgtagt
//
LOCUS       DATBSW010000190         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1040283, whole genome shotgun sequence.
ACCESSION   DATBSW010000190 DATBSW010000000
VERSION     DATBSW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040283"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VIF63_05320"
     CDS             complement(<1..317)
                     /locus_tag="VIF63_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238835.1"
                     /translation="MDEFTTPPSPLQAATLVRPGTAAWARVGLLGIAAAAILAAAIVA
                     FGAAAQPNGTLAAGTSSGVNDSPLVIDLNGLGGPGGRGGFGHGMGAVEITAISGSSIS
                     LKTV"
     gene            423..620
                     /locus_tag="VIF63_05325"
     CDS             423..620
                     /locus_tag="VIF63_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEY8238836.1"
                     /translation="MHDVRQIEPDEDPVCGMRVDPDAARAKGLVLSHDGRDYVFCGKG
                     CFLDFRDDPEPFLDPRRIPSM"
     gene            complement(630..1604)
                     /locus_tag="VIF63_05330"
     CDS             complement(630..1604)
                     /locus_tag="VIF63_05330"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEY8238837.1"
                     /translation="MIHAQAHKALIAIILGVGLLAAACGVGSGDLGPVATPPPTEQPS
                     LDVPSFEPTPGSTVDPSSEPSPGATGQPPETALPDGTTTIRAYFFLGSFVDNEGLAPV
                     LRVIPKTQSVGAAAMNALLAGPSEPELAARPAMYTVIPEGTRFLGLRVVSGIATVNLS
                     AEFESGGGSASVLGRLAQVVYTLTQFPTITGVKFELDGEPVTVFSGEGVALDSPVDRS
                     DYTDMLPAVFLDRPAWGGVPNNPMHIVGMANTFEAVFHFRLLDASGHKLAGGPLMATC
                     GTGCWGTFDVSIPYSVASAGWGTIQVWEPSAKDGSVENLTEYPVWLTP"
     gene            complement(1601..>2259)
                     /locus_tag="VIF63_05335"
     CDS             complement(1601..>2259)
                     /locus_tag="VIF63_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8238838.1"
                     /translation="DARRAAELLVADVRRLRVLIDDLMELSRFDAGAETIEATALEPT
                     DLVSTVRSIVASRLPEAELHVPGEAVVVDADVRRLDRIVGNLLDNARQHAPDSTVDVE
                     VAVEPGGGTAVVRVSDRGPGVAAEALPRLFDRFYKAEASRSRGSSGLGLAIAAEHAAL
                     LGGRLGAENREGGGLAVTLRLPVSRSLRAGDGPDTAEVEATGQSEAGLASGPPARPNP
                     "
BASE COUNT          333 a          776 c          832 g          318 t
ORIGIN      
        1 acggtcttca gggagatgct cgagccggag atcgcggtga tctcgacagc acccatgccg
       61 tgaccgaagc cgccgcggcc gccgggcccg ccgaggccgt tgaggtcgat cacgagcggg
      121 ctgtcgttca cgcccgagct cgtgcccgca gcgagggtgc cgttcggctg ggcggccgcg
      181 ccgaaggcca cgatcgcggc agcgaggatc gccgccgcgg cgatgcccag caagccgacc
      241 cgcgcccagg ccgcggtacc tggtcggacg agcgtggccg cctggagcgg gctcggcggt
      301 gtggtgaatt cgtccatggt gtctccgtca ttcgtgctcg ccgccgtatt cgtggcggtc
      361 cgtcagggag caccttgtcg gcctggcctg acaggatgct gagaacgcgc aacgataggg
      421 ccatgcatga tgttcgccag atcgagccgg acgaggatcc cgtctgcggc atgcgcgtcg
      481 acccggacgc ggcgcgagca aagggcctgg tcctcagcca cgacggccgc gactacgtgt
      541 tctgcggcaa gggctgcttc ctggacttcc gcgacgaccc cgagccgttc ctcgatccgc
      601 gacgcatccc ctcgatgtag ccggcgccgt tacggcgtga gccagaccgg gtactcggtg
      661 aggttctcga cggagccatc cttcgcggag ggctcccaga cctggatggt gccccagccg
      721 gccgacgcga ccgagtacgg gatcgagacg tcgaacgtgc cccagcagcc ggtaccgcag
      781 gtcgccatca acgggcctcc ggcgagcttg tggccgctcg cgtcgagcag gcggaagtgg
      841 aagacggcct cgaacgtgtt cgccatgccg acgatgtgca tcgggttgtt cgggacgccg
      901 ccccacgcgg ggcgatcgag gaacaccgcg ggcagcatat ccgtgtagtc ggatcggtcg
      961 accggcgagt cgagggccac gccctcaccg ctgaacacgg tgaccggctc gccgtcgagc
     1021 tcgaacttga cgccggtgat cgtcgggaac tgggtcagcg tgtacaccac ctgggcgagg
     1081 cggccgagga ccgacgccga tcccccaccc gactcgaatt cggcggacag gttgaccgtg
     1141 gcgatcccgc tcacgacgcg caggccgagg aagcgcgtgc cttcgggaat gacggtgtac
     1201 atcgcgggcc gcgcggcgag ctcgggctcg ctcgggccgg cgaggagcgc attcattgcg
     1261 gcggcgccga cggactgggt cttcgggatc acccggagca cgggcgcaag gccctcgttg
     1321 tcgacgaagc tgccgaggaa gaagtaggcg cggatcgtgg tcgtgccgtc gggcagcgcc
     1381 gtctctggcg gctggcccgt cgcgccgggc gacggctcgg aggatgggtc gaccgtggag
     1441 cccggagtcg gctcgaagct gggcacgtcg agcgagggct gttccgtggg cggcggcgtc
     1501 gcgaccggcc cgagatcgcc gctgccgacg ccgcaggcgg cggcgagcag tcccacgccg
     1561 aggatgatgg cgatgagggc tttgtgtgct tgtgcgtgga tcacgggttg ggcctcgctg
     1621 gtggaccgga cgccaggccg gcttccgatt gccccgtagc ctcgacctca gccgtatcgg
     1681 gcccatcgcc cgcccgtaac gatcgcgaaa ccggcagccg caacgtgacc gcgagcccgc
     1741 cgccttcgcg attctccgcg ccgagccggc cgcccagcaa cgcggcgtgc tcggcggcga
     1801 tggccagccc gagcccggag ctgcccctgc tccgcgaagc ttcggccttg tagaaccggt
     1861 cgaacaggcg cggcagcgcc tcggcggcga cgcccggacc gcgatccgac acgcggacga
     1921 ccgcggtccc gccgcccggt tcgaccgcga cctcgacatc gaccgtcgaa tcgggcgcgt
     1981 gctggcgcgc gttgtcgagc aggttgccga cgatgcgatc gagccggcgg acgtccgcgt
     2041 cgaccacgac cgcttcgcct ggaacgtgca gctcggcctc cggcaggcgg ctggcgacga
     2101 tcgatcggac ggtcgagacc aggtcggtgg gctccagggc agtcgcctcg atcgtctcgg
     2161 cacccgcgtc gaagcgcgac agctccatga ggtcatcgat caggacgcgc agccggcgga
     2221 cgtcggccac gagcagctcg gcggcgcggc gcgcgtcgg
//
LOCUS       DATBSW010000191         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_652107, whole genome shotgun sequence.
ACCESSION   DATBSW010000191 DATBSW010000000
VERSION     DATBSW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652107"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VIF63_05340"
     CDS             complement(<1..288)
                     /locus_tag="VIF63_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8238839.1"
                     /translation="MSIETTSARMTSEEIVALNRAHTFFSWSVQGALDPIAIDHAEGV
                     YLYTPDGTRILDFNSQLMSVNIGHGDRRVINAITEQATKLQYVMPAFATEIR"
     gene            453..1226
                     /locus_tag="VIF63_05345"
     CDS             453..1226
                     /locus_tag="VIF63_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238840.1"
                     /translation="MSNWVMRRLGVSATFLLLVLAFPAAAGATITGGCTGIGTSTSSS
                     VDLTTETEWHVKKADSGGGAGQGPAAHAASVGAYALGIVVPIANGTSEPGETSGSVEG
                     VSVQLFAVLGARFVVSGKADNGCAGQIKIIIDDQDPLYTVLGGGGLLLAVIGGLLVLR
                     TMRGGKGLFKRLLDAIYGLVGGLGAALALEQFGYIDPTSVLGLLLVIVAGIFAFLTCG
                     ILGGGKKPPVVTEPVPPSTPVGGGTETYPSGGVGGGDAT"
     gene            complement(1306..1905)
                     /locus_tag="VIF63_05350"
     CDS             complement(1306..1905)
                     /locus_tag="VIF63_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY8238841.1"
                     /translation="MHSHRIHRHALLGAATLLVALALPASTLAASYTGTPGPDHIMGT
                     ADADQIAGLAGNDVLEGLGGPDAIRGGDDNDLIYGGDGNDALGGGNGNDGIRGGEGRD
                     VANGGDGADKVAGGPGADALYGGAGPDILWGGDGPDVIWGGAGRDLAYGGAGNDVIHV
                     GRDKTADAVHCGPGWDVAYLGRYDIADASCERTFRNLED"
     gene            complement(2018..2671)
                     /locus_tag="VIF63_05355"
     CDS             complement(2018..2671)
                     /locus_tag="VIF63_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8238842.1"
                     /translation="MTIRVLIADDERLVRAGFRMILASEPGIEVIGEARDGREAIEAV
                     HRLHPDVVLMDVRMPGVDGLAATREIVTGAGAPARVIVLTTFDLDEYVYGALQAGATG
                     FLLKDAPEAQLVAAIRAAATGGALFSPEATQRLVATFARRPPARDESTTADLTARERE
                     VLLLVATGLSNREIAETLSITEHTAKTHVAHILGKLGLRDRVQAVVFAYEHDLVPRP"
     gene            complement(2668..3885)
                     /locus_tag="VIF63_05360"
     CDS             complement(2668..3885)
                     /locus_tag="VIF63_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8238843.1"
                     /translation="MRAREAFGNHAWTILAIVLTAIAQVELWLLWQPDVDEATGPVSI
                     GVRALAAAGTLPLTLSLALGRRWPLLPLALAIPAIAVLPAGPLEVSVAVVGAIVVAAL
                     AAALLAPSATEIGIGAGGIAALVVTTAIAYPDTVEEPGDLALLVAALVGPWLAGLAIR
                     ARRERETALESLAVRAEASRQADAQRAVTDERARIARELHDVVAHAISVIVLQARGGR
                     RAIASDPAAATQALDDIEMTGSRALAEMRRLVGVLRPGDAANPGAGALEPPPGLGALE
                     ALVAGVRDAGLEVTVQVEGTPVELAPGVDLAAYRLVQEAFTNTLRHAEAVQAKVILRY
                     VPGAIEIEVVDEGRPEPAAGQRPQPADVASGHGMIGMRERAALYGGSVEFGPRPDGGF
                     RVLARLPVGGDPA"
     gene            4017..>4930
                     /locus_tag="VIF63_05365"
     CDS             4017..>4930
                     /locus_tag="VIF63_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238844.1"
                     /translation="MSNRTTSNRTANSETCATRATRVTRATGAFAVAIGLAGAVAGCL
                     GGPSTSSSPTTVPTVVAPSAVTPPTTLPTAVPSPTVEPAAFPRNPAPYVEGAAYAQVF
                     NPADFVAGIDNPFLPFTPGARFVFDGDEHVEVEVLAETKQILGIAATVVRDQVFDGDE
                     LIEDTLDWYAQDRDGNVWYMGEQTAEYENGKVSSTAGSWEGGVDGAQPGIVMLADPQA
                     GDTYRQEFYAGEAEDVAAITAVTGEVTVPAGTWSGADVLVTEEWTPLEPDVRERKTYA
                     RGYGVVEIRTVKGGDEMTTLTSMTTASLA"
BASE COUNT          706 a         1765 c         1747 g          712 t
ORIGIN      
        1 ccggatctcc gtcgcgaacg cgggcatgac gtactggagc ttcgtcgcct gctcggtgat
       61 cgcgttgatc acgcggcggt cgccgtgacc gatgttcacc gacatcagct ggctgttgaa
      121 gtcgaggatc cgggtgccgt cgggcgtata caggtagacg ccctcggcgt ggtcgatggc
      181 gatcgggtcg agcgcgccct gcaccgacca cgagaagaag gtgtgggccc gattgagggc
      241 gacgatctcc tcgctcgtca tccgggcgct cgtcgtctcg atgctcatca cgacctcacg
      301 ctgggctcga ccgatttggc tcgaaggtgg cccgaccatt agaccacgag gcacgcagcc
      361 cacgcgacgt gcgggccggg gattcgcctc ttgacggccg ccgatccggc catacgctgc
      421 cgcgcaaggg gttctgaggg gaggaggcca tcgtgtccaa ctgggtcatg cgccgactcg
      481 gggtcagcgc gacgttcctg ctgctcgtgc tggcgttccc ggcggccgcc ggggcgacga
      541 tcacgggcgg ctgcaccggt atcggtacgt ccacgagctc gagcgtcgac ctgacgaccg
      601 aaaccgagtg gcacgtcaag aaggccgaca gcggtggcgg cgccggccag ggcccggcgg
      661 cccacgccgc ctcggtgggc gcgtacgcgc tgggcatcgt ggtcccgatc gcgaacggga
      721 cgtcggagcc cggcgagacg tccggctcgg tcgagggcgt cagcgtgcag ctgttcgccg
      781 tgctcggcgc gcgattcgtg gtctccggca aggcggataa cggctgcgcc ggccagatca
      841 agatcatcat cgacgaccag gatccgctct acacggtgct cggcggcggc gggctgctcc
      901 tcgccgtcat cggcggcctg ctcgttctcc ggaccatgcg cggcggcaag ggcctgttca
      961 agcgcctgct cgacgcgatc tacgggctcg tcggtggcct cggcgcggcg cttgcgctcg
     1021 agcagttcgg ctatatcgat ccgacgtccg tcctgggcct gctgctcgtg atcgtcgccg
     1081 gaatctttgc gttcctcacc tgcggcatcc tcggcggcgg caagaagccg ccggtcgtca
     1141 ccgagccggt cccaccatcg acgcctgtcg gcggcggcac ggagacgtac ccgagtggcg
     1201 gtgtcggcgg cggtgacgcc acctgacccc acgttcctct gaatcagcga ggccgccggc
     1261 gatgccggcg gcctcttcgt ttgcagttgg accgagctcg acggctcagt cctcgaggtt
     1321 tcggaacgtc cgctcgcagc tcgcgtcggc gatgtcgtag cggccgaggt acgcgacgtc
     1381 ccaacccgga ccgcagtgga ccgcgtcggc cgtcttgtcg cgcccgacat ggatgacgtc
     1441 gttgccggcg ccgccgtagg ccaggtcgcg gcccgcgccg ccccagatca cgtccggccc
     1501 gtcgccgccc cacaggatgt cggggccggc gccgccgtag agggcatcgg caccggggcc
     1561 gccggcaacc ttgtcggcgc cgtcgccgcc gttggccacg tcccggccct cgccgccgcg
     1621 gatgccgtcg ttgccgttcc cgccgccgag ggcgtcgttg ccgtcgccgc cgtagatcag
     1681 gtcgttgtca tcgccgccac gaatggcgtc ggggccaccc aggccctcga ggacgtcgtt
     1741 gccggcgagt ccggcgatct ggtccgcatc ggccgtgccc atgatgtggt ccgggcccgg
     1801 cgtccccgtg tagctcgccg ccagcgtcga tgccggcagg gcgagggcca cgagcaacgt
     1861 cgcggcgccg agcagtgcgt gtcgatggat tcggtggctg tgcatcgtca gtgctcccgt
     1921 gctgctgtcc gattggtggg ccgagcctag tccgccgggt cggcgttggt ctgcagccgg
     1981 acgtgccgtt gcgtaagcac cccgtaaggg cgaagcgtca cggtcgcggg acgagatcgt
     2041 gctcgtaggc gaacaccacc gcctgcaccc gatcacgcag gcccagcttg ccgaggatgt
     2101 gggcgacgtg cgtcttggcc gtgtgctcgg tgatcgagag cgtctcggca atctcccggt
     2161 tcgagaggcc cgtcgcgacg aggaggagca cctcgcgctc ccgtgcggtg aggtccgccg
     2221 tcgtggactc gtcgcgcgcc ggcggccgcc gggcaaacgt tgcgaccagt cgctgcgtgg
     2281 cttccggcga gaagagcgct ccacccgtgg ccgccgcccg gatcgcggcg acgagctgcg
     2341 cttccggcgc gtccttgagc aggaagcccg tcgcgccggc ctgcagcgcg ccgtagacgt
     2401 attcgtcgag gtcgaacgtc gtcaggacga tgacgcgggc aggtgcaccg gcgccggtga
     2461 cgatctcgcg ggttgctgcg agcccatcga cgccgggcat tcggacgtcc atcagcacca
     2521 catcgggatg cagccggtgc acggcttcga tcgcttcgcg tccgtcccga gcctcgccga
     2581 tgacctcgat cccgggctcc gaggcgagga tcatccggaa gccggcgcgc acgaggcgct
     2641 cgtcgtcggc gatcaggacg cggatcgtca cgccgggtca cctccaacgg ggagccgcgc
     2701 cagcaccctg aacccaccgt ccggacgtgg cccgaactcg acgctgccgc cgtagagggc
     2761 ggcacgctcg cgcatgccga tcatgccgtg accggaggcg acgtcagcgg gctgggggcg
     2821 ctggcccgcc gccggctccg ggcgcccctc gtcgacgacc tcgatctcga tcgctccggg
     2881 cacgtatcga aggatgacct tggcctgcac tgcctcggcg tggcggagcg tgttggtgaa
     2941 cgcctcctgg acgagccggt acgcggcgag gtccacgccc ggggccagct cgacgggcgt
     3001 tccttcgacc tggaccgtca cctcgagacc agcgtcccgt acccctgcga cgagggcctc
     3061 gagcgctccg agccccggtg gtggctccag cgcgcctgcc cccggattgg cggcgtcacc
     3121 gggccgcagc acgcccacga gccggcgcat ctcggccagg gcgcgagatc ccgtcatctc
     3181 gatgtcatcg agcgcctggg tcgcggccgc gggatcgctg gcgatggccc gccgcccgcc
     3241 gcgcgcctgg aggacgatca cgctgatcgc gtgcgcgacc acgtcgtgca gctcccgcgc
     3301 gatccgggcc cgctcgtccg tgacggcgcg ctgggcatca gcctgtcgcg acgcctctgc
     3361 ccggaccgcg agggactcga gtgccgtctc gcgttcacga cgggcccgga tcgcgagccc
     3421 cgccagccac ggcccgacaa gggcggcgac gagcaaagcc aggtcgcccg gttcttcgac
     3481 cgtgtcggga taagcgatgg ccgtcgtgac gacgagcgcg gcgatgccgc cggcgccgat
     3541 cccgatctcg gtcgcgctcg gagccagcaa cgccgccgcc aatgcggcaa ccacgatggc
     3601 gccaacgacc gcgacggaca cctcgagcgg acccgcgggc aagaccgcga tggctgggat
     3661 cgcgagcgcg agcggcagga gcggccatcg gcgcccgagg gcgagcgaca acgtgagcgg
     3721 gagcgtccca gctgccgcaa gcgcgcgcac gccgatcgac accgggccgg tcgcctcgtc
     3781 cacgtcgggc tgccagagca gccagagctc gacctgggcg atcgccgtca gcacgatcgc
     3841 gaggatggtc caggcgtggt tcccgaatgc ctcgcgcgca cgcatgccgg caggctacgc
     3901 ccgcttcgcg ccggcggcgt cactcgaatg gatgacgccg catccggctg gcgagggacg
     3961 acagccgtcc tgatcgagcg tacggtcgtc ccgatcgccg ctcctggagg ccagccatgt
     4021 cgaaccggac cacgtcgaac cgcaccgcga acagcgagac ctgcgcaacc cgcgcgaccc
     4081 gcgtcacgcg cgcgaccggc gcctttgccg tcgccatcgg actggccggc gcggtcgccg
     4141 gctgcctcgg cgggccatcc acgagctcct cgccgaccac cgtgccgacg gtggtggcgc
     4201 catcggccgt gacgccccca acgaccctgc cgaccgccgt ccccagccca accgtcgagc
     4261 ccgcggcatt cccgcgcaat ccagcgccct acgtcgaggg cgccgcgtat gcgcaggtct
     4321 tcaaccccgc tgacttcgtc gccggcatcg acaacccgtt cctgccgttc acgccggggg
     4381 cgagattcgt gttcgacggt gacgagcacg tcgaggtcga ggtcctggcc gagaccaagc
     4441 agatccttgg catcgcagcg acggtcgtgc gcgaccaggt gttcgacggc gacgagctga
     4501 tcgaggacac gctcgactgg tacgcccagg accgcgacgg caacgtctgg tacatgggcg
     4561 agcagacggc cgagtacgag aacggaaagg tctcgtcgac ggcaggctcg tgggagggcg
     4621 gcgtcgacgg cgcgcaaccc gggatcgtga tgctggccga tccccaggcc ggcgacacct
     4681 accgccagga gttctacgcc ggcgaagcgg aggacgtcgc agcgatcacg gccgtgacag
     4741 gcgaggtcac cgtccccgcg ggaacctggt ccggcgcgga cgtcctcgtc accgaggaat
     4801 ggaccccgct ggaaccggat gtccgcgagc gcaagacgta cgcgcggggc tacggcgtcg
     4861 tcgagatccg aacggtcaag ggcggcgacg agatgacgac cctcacgagc atgaccaccg
     4921 cgagccttgc
//
LOCUS       DATBSW010000192         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1040335, whole genome shotgun sequence.
ACCESSION   DATBSW010000192 DATBSW010000000
VERSION     DATBSW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040335"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIF63_05370"
     CDS             complement(<1..378)
                     /locus_tag="VIF63_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY8238845.1"
                     /translation="MATAQVPTERAHKAAKRYIYAWGDGAAEGSGTMKDLLGGKGAGL
                     AEMTRAGLPTPPGFTITTAACNDYFAAGEKLPDGLWDDVLEALHDVERQTGKGFGDSK
                     NPLLVSVRSGAKFSMPGMMDTVLN"
     gene            complement(477..740)
                     /locus_tag="VIF63_05375"
     CDS             complement(477..740)
                     /locus_tag="VIF63_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238846.1"
                     /translation="MGTLLLPSAVLADAYGDWRDTAFIVGMIIAPGAALLAAALAQSF
                     AAPPPPPDLLAARTSPVPNIPPIPGGDIPPPPTNPPPPSKGTA"
     gene            complement(805..2316)
                     /locus_tag="VIF63_05380"
     CDS             complement(805..2316)
                     /locus_tag="VIF63_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238847.1"
                     /translation="MRALPPVLGAILLPLSAVLVPLVFSIGTATTEPFVQADPYDLFN
                     FLIYDVFTESIPFMLLGIAVGFLSPAAGVLFVVAYGIGNVAATIVTNELDPLLPALYG
                     RFASCVILWLLVVEIPLTSRAGFERLSRGMAARGHLIAIGGASLLATLLLSAFAVTAA
                     MLVGVVFWLTRSWGSGPTLRASNTLEVSPEVLIVPAAILAVLLLYLRYPSPDAPFGSA
                     EPAGPPPSPAQRAIRYGISVGLALTLLAGAMSQVIDLVVLVVGLILGRPIAQAILRAT
                     RLAAPLARIPWLPRVGLGAAVSIAFSGALLSLLGYPEISRWFPVVVAIAGSYVILQIM
                     LEADQVAAAVGSPHARPDAAVAVGSGLAVLSFMQLSAFALPAPVFADAFGDWRDTAFI
                     IGMIIAPGAAMSAAMLMGQLAGGLEEDKPGKSPPGYLRRAPHGGLPGANAGGGAGRPW
                     RSAGPTKDGGWDPGTGPNAPRGPVPRESDPDPPADPPPPPPPPPAPPPGPNVS"
     gene            complement(2415..3092)
                     /locus_tag="VIF63_05385"
     CDS             complement(2415..3092)
                     /locus_tag="VIF63_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238848.1"
                     /translation="MARLRFGSGFDVRYAVAFLALFSGIALLLNIIFSVDLRLGLGAL
                     VILVVGAFASLLRRASDAERRTILRTLAYGAVAGVIGTLVYDVARTILAIIDQSPYRP
                     FEAIVVFGQLLLGSQARDAGVILTGGLFHILNGSSFGVAFTFVAVTRHRIGIKRLIAL
                     GVGWGLFLESFQMLLYPDWLGITYFREFLTISALGHVAYGATLGPTARALLSRGMARP
                     SGGEAVA"
     gene            3181..4017
                     /locus_tag="VIF63_05390"
     CDS             3181..4017
                     /locus_tag="VIF63_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8238849.1"
                     /translation="MIETDERVILPDAPQYNASLVARTDETESLGYFVVRFDGDATPF
                     ESGQYMTIGVFVEDATTPTGTKIVQRPYSIASDPFVAGSDGYEMYIRLVQGGMFTPLL
                     WRLPVGHRMRMIGPKGKFVLEPDDDRTHLFISSGTGNAPFVSMMKAMLRVGQPRKAVF
                     LNGVSYAGDLGYRALLEGWESGGGYPVQYIPTVSRPQAPENASWTGRWGRVESIVAPV
                     CDELGLTADNTIAYICGNPDMILAAEETLLGRGFPEPQVKKELYWPKGKEPTGGGTST
                     DE"
     gene            complement(4025..>4500)
                     /locus_tag="VIF63_05395"
     CDS             complement(4025..>4500)
                     /locus_tag="VIF63_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY8238850.1"
                     /translation="TASAEFNIWLDPEAARIVVNCGRPIRMVPLDATHRALVSTEDAA
                     RLRALGTPAGEAAARFVLQRINAYDATQPMPRRGDAQAAPVHDALAVCAIIDPSILAT
                     EHIPVDVEIHAQLSLGRTVCDFRFRSGKPANVHFATDADEPKFNRMLLEILGRTA"
BASE COUNT          796 a         1475 c         1605 g          624 t
ORIGIN      
        1 gttcagaacg gtgtccatca tccccggcat cgagaatttc gcgcccgagc gcacgctgac
       61 gaggagcggg ttcttcgaat ctccgaagcc cttgccggtc tggcgctcga cgtcgtggag
      121 cgcctcgagg acgtcgtccc acaggccgtc agggagcttc tcgccggcgg cgaagtagtc
      181 gttgcaggcg gcggtcgtga tcgtgaagcc gggcggggtc ggcaggccgg cgcgggtcat
      241 ctcggcgagg cccgcgccct tgccgccgag gaggtccttc atcgtcccgc tgccctcggc
      301 cgcgccgtcg ccccacgcgt agatgtagcg cttggcggcc ttgtgggcgc gttcggtcgg
      361 tacttgggca gtggccatgc gggcggagcc tcctcggtgc ttgcggtgcg aaggattgta
      421 cgagggatgg aagggggccc ggtcggggcg ggatcaggtc ctccgagcgc ctcgggtcac
      481 gcggttccct tactcggcgg cggcgggttg gtgggcgggg gcgggatatc cccaccaggg
      541 attggcggga tgttcggaac aggagacgtc cgggccgcga gcagatccgg cgggggaggc
      601 ggggctgcga acgactgcgc caacgcagcg gccaggagcg cggcgccagg cgcgatgatc
      661 atcccgacga tgaacgcggt gtcgcgccag tcgccatacg cgtcggccag caccgccgat
      721 ggcagcagca gcgtgcccac ggtgatgatg gccaaggcga ttccgaccgg acggcgaata
      781 cgcttgttcc tcaccgacaa ccgttcagga cacattcggg cccggcggcg gtgcgggcgg
      841 cggcgggggc ggcggtggcg gatcggcagg tggatccgga tcggactcgc ggggcaccgg
      901 accccgcggt gcatttggcc cggtacctgg gtcccaaccg ccgtccttcg tcggcccggc
      961 cgaccgccat ggtcgcccgg cgccgccgcc ggcattcgca cctggcaaac cgccatgagg
     1021 agctcggcgc agatagccag gcgggctctt tccgggcttg tcctcctcca ggccgcctgc
     1081 gagctgcccc atcagcatcg ctgcgctcat ggcggcgccg ggcgcaatga tcatgccgat
     1141 gatgaacgcg gtgtcacgcc agtcgccgaa cgcatcggcg aagacgggcg cggggagcgc
     1201 aaacgcggag agctgcatga acgagagcac ggccaagccg gaaccgacag ccacggctgc
     1261 gtcgggcctg gcatgtggcg agccgactgc cgcggccacc tgatccgcct cgagcatgat
     1321 ctggaggatc acgtacgagc ccgcaatcgc gacgaccacg gggaaccagc gcgagatctc
     1381 cggatagccg agaagcgaaa ggagcgcgcc tgagaatgca atcgagacgg ctgcccccag
     1441 tccaacgcga gggagccacg ggatgcgcgc caagggggcc gcgagccgag tggcccgaag
     1501 gatcgcctgc gcgatcggcc tgccgaggat caacccgacg acaaggacaa ccaggtcgat
     1561 gacctggctc atcgccccgg cgagcagggt cagcgcgaga ccaaccgaga tcccgtatcg
     1621 aatcgcgcgc tgcgctggcg atggtggcgg tcctgccggt tcggcgctcc cgaacggggc
     1681 atccgggctc ggatagcgca gatacagcag cagcactgcg agaatcgccg ccggcacgat
     1741 cagcacctcg ggcgagacct cgagcgtgtt cgacgcccgc agcgtcgggc cgctgcccca
     1801 gctcctcgtg agccagaaca cgacgcccac gagcatggcg gccgtcacgg cgaacgccga
     1861 caacagcagc gttgccagga gcgaggcgcc gccgatagcg atcaggtggc cgcgggctgc
     1921 catgccgcgg ctgagtcgct cgaatccggc ccgactcgtc agcgggatct cgacgacgag
     1981 cagccagagg atgacgcagg acgcgaaccg gccatacagc gccggaagca acggatcgag
     2041 ctcgttcgtc acgatcgtgg ctgcaacatt gccgatgccg tacgcgacga caaacaggac
     2101 gccggcagcc ggcgacagga agccgacggc gatccccagg agcataaacg ggatcgattc
     2161 ggtgaagacg tcgtagatca ggaagttgaa cagatcgtag ggatcggcct ggacgaatgg
     2221 ctcggtcgtc gccgtcccga tcgagaacac cagcgggacc aggaccgcgc tcagcggaag
     2281 caggatggcg ccgagaaccg gaggcaacgc tctcatgcgc cgagcgtcgg cgtccgtcgc
     2341 ggcccgaagg ccaggtcgca ccaattcgag gtgtcgggcg ggccacatcc agcgatacgg
     2401 actcgcggct tccatcatgc gacggcctcc cctcccgatg gtcgcgccat gcccctcgac
     2461 aatagcgctc tcgcagtcgg cccgagcgtc gcgccgtagg caacgtggcc gagcgcagag
     2521 atcgtcagga actcgcggaa gtaggtgatc cccagccagt ccgggtacaa cagcatctgg
     2581 aacgactcga ggaagaggcc ccagccgacg ccgagcgcga tgagccgctt gatgccgatc
     2641 cgatggcgcg tcaccgcgac gaaggtgaag gcgacgccga acgaggaccc gttcaggatg
     2701 tgaaacagcc caccggtgag gatcacgccg gcatcgcgcg cctgcgaccc aagcaagagc
     2761 tggccgaaca caacgatggc ctcgaagggc cgatacggac tctggtcgat gatcgccagg
     2821 atcgtccgag cgacgtcgta caccagtgtg ccgatcacgc cggcgacggc tccataggcc
     2881 agcgtgcgca ggatcgtccg tcgctccgcg tcgctcgctc ggcgaaggag ggaggcaaag
     2941 gcgccgacga ccaggatcac cagagctccg agtccaagtc gaagatcgac gctgaagatg
     3001 atgttcagga ggagcgcgat tccgctgaac aaggcgagga acgcgactgc atagcggacg
     3061 tcgaagccgg atccgaaccg tagccgggcc acgagcgcat ggtgccctgc ctgacgatcc
     3121 gtttcatcgt ctacctgcgc caacggtgat cgcggcggga cgggccgcta ccatcgtggg
     3181 gtgatcgaga ccgacgagcg cgtcatcctg cccgacgcgc cgcagtacaa cgcgtccctc
     3241 gttgcccgta cggatgagac cgagagcctc ggctacttcg tggtccggtt cgatggcgac
     3301 gcgacaccgt tcgagtccgg ccagtacatg acgatcggcg tcttcgtcga ggacgcaacg
     3361 acgccgaccg ggaccaagat cgtccagcgg ccgtactcga tcgcgtcgga tcccttcgtc
     3421 gccggatcgg atggctatga gatgtacatc cggctcgtcc agggcgggat gttcacgccg
     3481 ctgctctggc ggctgcccgt cgggcaccgc atgcggatga tcgggccgaa gggcaagttc
     3541 gtcctcgaac cggacgacga ccggacgcac ctgttcatct cgtccggcac gggcaacgcg
     3601 ccgttcgtgt cgatgatgaa ggcgatgctg cgggtcgggc agccgcggaa agcggtcttc
     3661 ctcaacggcg tctcgtacgc cggcgacctc ggctaccggg cgctgctcga gggctgggag
     3721 tcgggcggcg gctaccccgt ccagtacatc cctacggtct cacggccgca ggcgcccgag
     3781 aacgcctcgt ggaccggccg atggggccgc gtggaatcga tcgtcgcgcc ggtctgcgat
     3841 gagctcggcc tgacggcgga caacacgatc gcctacatct gcggcaatcc cgacatgatc
     3901 ctcgccgccg aggagacgct cctgggtcgc ggcttccccg agccgcaggt caagaaggag
     3961 ctctactggc cgaagggcaa ggaaccgacc ggcggcggga cctccacgga cgagtaggtc
     4021 cgcgctacgc cgtgcggccg aggatctcca gcagcatccg gttgaacttc ggctcgtcgg
     4081 cgtcggtcgc gaagtggacg ttcgcgggct tgccgctccg gaagcggaaa tcgcacaccg
     4141 ttcgcccgag cgacagctgg gcgtggatct cgacgtcgac cgggatgtgc tccgtcgcga
     4201 ggatcgaggg atcgatgatc gcgcagacgg cgagcgcatc gtgcacgggc gccgcctgcg
     4261 cgtcgccgcg gcggggcatc ggctgggtgg catcgtaggc gttgatccgc tgcaggacga
     4321 agcgcgccgc agcttctccc gcgggcgtgc cgagggcccg aaggcgcgcg gcgtcctcgg
     4381 tcgagacgag cgcgcgatgg gtggcgtcga gcgggaccat ccggatcggg cgaccgcagt
     4441 tcacgacgat gcgggccgcc tcgggatcga gccagatgtt gaactccgcc gaggcggtga
//
LOCUS       DATBSW010000193         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_652130, whole genome shotgun sequence.
ACCESSION   DATBSW010000193 DATBSW010000000
VERSION     DATBSW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652130"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..788
                     /locus_tag="VIF63_05400"
     CDS             114..788
                     /locus_tag="VIF63_05400"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY8238851.1"
                     /translation="MHRRRNQLTIAAVAFILGLLVVVQLRAQSTGTGLAALSAQDLTV
                     LVANLNERNDQLRREVASLEGELATLTANQGRGDASIDEIRMDLRRVRAYAGLDPVVG
                     PGVSIQISGPIDSDGLDDLINELRNSGAEAIAVDGRRIVPGLVVDGGPGSISVDGESL
                     ADPFELVAVGASEQLTGSLTRSGGVIAQLAATLPDVVLTVTPLDRIELPATTRDLIPD
                     NGHPRL"
     gene            804..1052
                     /locus_tag="VIF63_05405"
     CDS             804..1052
                     /locus_tag="VIF63_05405"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEY8238852.1"
                     /translation="MNEPAVLTLYVGDVVRLRRSHPCGSDTWLVDRLGADIGLRCEGC
                     GRHILLERPVLERRIAAFVSRGDAAMSAALAPPTGPAP"
     gene            1049..>2244
                     /locus_tag="VIF63_05410"
     CDS             1049..>2244
                     /locus_tag="VIF63_05410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8238853.1"
                     /translation="MTVAETAPDPTEELPSVFRNGAFLRLWLSQAATQIGGNMVLYGL
                     TVIVLSTTGLNTVVSLLILTFLVPAVLFSAVAGVYVDRIDRRTVLVATNLIRGVAFIA
                     LYLIGSNLALILLLNAFISTVTVFFAPAEAAMIPQVVDRRQLQTANGIFTITLNAAFA
                     IGFALLGPLVVNLASPEAVILVVAVLYLIAAAFCFALPKSPPPGSLGASESLHAAERA
                     AGSTMAQLREGLGFIRANRTIGWSLIYLGITASLVGVLGVLGPDFAKETLGLEAKGFA
                     VVVLPLGFGIVTGILLLNAYGHLLPRRRVIEGGLIALGLLLALMSIAGPISRFLQRAD
                     APGGLDLSGVTSLLAVVVVIALLAGIAYGCVAIPAQTQLQEDLPEDVRGRVFGVLNML
                     VSVSSFV"
BASE COUNT          273 a          844 c          735 g          392 t
ORIGIN      
        1 gtcgacgtgc ccgccttcgc cggcacgatc tcgctgcgct actcgcgacc ggtgccgtcg
       61 gaatcgccgc cccccgatgg cggtgccccg tcaccgtccc cgagcggctg accatgcatc
      121 gccgccgcaa ccagctgacc atcgccgcag tggccttcat cctcggcctg ctcgtcgtcg
      181 tccagcttcg ggcgcagtcc accgggaccg gtctggcggc cctgtccgcg caggacctga
      241 cggtgctggt cgccaacctc aacgaacgaa acgaccagtt gcgccgcgag gtcgcgtccc
      301 tcgagggcga gctggccacg ctcaccgcga accagggtcg tggcgacgcg tccatcgacg
      361 agatccggat ggacctgcgt cgcgtgcgcg cctacgccgg gctcgatccc gtcgtcgggc
      421 cgggcgtgtc gatccagatc agcggcccga tcgacagcga cggcctcgac gacctcatca
      481 acgagctgcg caacagcggc gcggaggcca tcgccgtcga cggtcggcgc atcgtccccg
      541 gtctcgtcgt ggacggcggg cctggttcca tctcggtcga cggcgagtcg ttggccgatc
      601 cgttcgagct cgtcgccgtc ggggcgtcgg agcagctgac cggttccctg acccgctcgg
      661 gcggcgtgat cgcccagctg gccgcgaccc tgcccgacgt cgtgctgacc gtcacgccgc
      721 tggaccgtat cgagctgccg gcgacgacgc gcgacctcat cccggacaac ggccatcccc
      781 ggctttgata cgctgacccg ccgatgaacg agccagccgt attgacgctg tacgtgggcg
      841 acgtcgtgcg cctgcgtcgc tcgcacccct gcggcagcga tacctggctg gtcgaccggc
      901 tcggggcgga catcggcctg cgctgcgaag gctgtggccg gcacatcctc ctggagcgtc
      961 ccgtcctcga gcgccgcatc gcggccttcg tgtcgcgcgg cgatgcggcg atgagcgcag
     1021 cgctcgcccc gccgacgggt cccgccccgt gacggtcgcc gagacggcgc cggacccgac
     1081 ggaggagctt ccgtcggtct tccgcaacgg cgcgttcctc cgcctctggc tgtcgcaggc
     1141 cgcgacccag atcggcggca acatggtcct ctacggcctg accgtcatcg tcctgagcac
     1201 caccgggctc aatacggtcg tcagcctgct gatcctgacc ttcctcgtgc cggcggtgct
     1261 gttctccgcg gtcgccggcg tctacgtcga tcggatcgat cggcggaccg tgctcgtcgc
     1321 gacgaacctg atccgcggcg tcgccttcat cgcgctttac ctgatcgggt ccaacctcgc
     1381 cctgatcctc ctcctcaacg cgttcatctc gaccgtcacc gtgttcttcg ccccggccga
     1441 ggcggcgatg atcccgcagg tcgtcgaccg ccggcagctc cagacggcca acggcatctt
     1501 cacgatcacc ctcaacgcgg cgtttgcgat cgggttcgcg ctgctcggtc cgctggtcgt
     1561 caatctcgcc tcgccggagg cggtcatcct ggtcgtggcc gtcctctacc tgatcgcggc
     1621 cgccttctgc ttcgccctgc cgaagtcgcc gccacctggc tcactcggcg cgagcgagtc
     1681 actccatgcc gcggagcgcg ccgcgggctc gacgatggcc cagctgcgcg aaggcctcgg
     1741 gttcatccgg gcgaatcgta cgatcggctg gtccctcatc tacctgggga tcaccgcgtc
     1801 gctggtcggc gttctgggcg tgctcggccc ggacttcgcc aaggagacgc tcgggctcga
     1861 ggccaagggc ttcgccgtcg tggtcctgcc gctggggttc ggcatcgtca ccggcatcct
     1921 cctgctgaac gcgtacggcc acctcctacc gcgtcggcgg gtgatcgagg gcgggctcat
     1981 cgcgctcggc ctgctcctcg cgctgatgtc gatcgcggga cccatcagcc ggttcctgca
     2041 gcgcgccgac gcccccggcg ggctcgacct gtccggggtg acatcgctgc tggcggtcgt
     2101 cgtggtcatc gccctgctcg cgggcatcgc ctatgggtgc gtggcgatcc ctgcccagac
     2161 gcagctccag gaggacctcc ccgaggatgt ccgggggcgc gtcttcggcg tgctgaacat
     2221 gctcgtgtcc gtctcgagct tcgt
//
LOCUS       DATBSW010000194         5897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1216411, whole genome shotgun sequence.
ACCESSION   DATBSW010000194 DATBSW010000000
VERSION     DATBSW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216411"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VIF63_05415"
     CDS             <1..264
                     /locus_tag="VIF63_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238854.1"
                     /translation="RAEAAVIRFEATRVYSRAWTPDAARRLEALRIRAGRIVNTSPRT
                     RYDAVLATLGREVEAERRSVHVQRRTVDLDRERPPVDLERRPA"
     gene            309..1151
                     /locus_tag="VIF63_05420"
     CDS             309..1151
                     /locus_tag="VIF63_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HEY8238855.1"
                     /translation="MDAEAEATPLDPGLTGQFARPVPGVDNGYLAGYRVRFDEAAGDG
                     LMRTSALMRYAQDIAWRHSEQLGFDRDWYAERGLGWVVRGVELELHAPVPMGHTLRLT
                     TAVVGHRRIWARRLGECRLADGRLAARVTTDWVLLDGRNRIVRIPADFGVAFQNPELD
                     SEIIRVAAPQEEPAHTLALAVGPRDLDPLAHVNNAVYVDWLDEALETAGWSGTARPEG
                     STLRLEYLASAERGNQVAVELYGTAADWVARIRRADGQELVRASGAAGAVDTAAAPRA
                     ASRG"
     gene            1183..2445
                     /locus_tag="VIF63_05425"
     CDS             1183..2445
                     /locus_tag="VIF63_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8238856.1"
                     /translation="MTTLVDEYLARTPGSAAMFERAARSLPGGSTRTTIYSAPYPPYM
                     VRGSGISTWDVDGNEYRDFLGNYTSLILGHAHPEVVAAVEAQVRRGSAFAAPTETEIE
                     LAEEIRRRLPSIEAIRFTNSGTEATMFALRAARAFTNRPLIAKFELAYHGTHDTAGAG
                     TPGVPRATSDLVVELPWGDPEGVERALAGREPEVAAIIIEPVQGAGGVRAATPEFLNF
                     LRALTIRIGALLILDEVIAFRIGPNGAQGRLGGIRPDLTTLGKIIGGGYPSGAFGGRT
                     DVMAQFDARRSGALVHGGTFNGNPVSASAGLATLRHLTPERYVELDRLGDRLRAAIAD
                     GVARAGLDASGGGVASIFQVVPGSSLVPADGLSPQAALFIGLLLDGFHLAPRGMGALS
                     TVTTDADVDDLAQAVVARLGAMQAVAAA"
     gene            complement(2447..3685)
                     /locus_tag="VIF63_05430"
     CDS             complement(2447..3685)
                     /locus_tag="VIF63_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEY8238857.1"
                     /translation="MTDAATPVQAPSPTTVATPPPARSGGPSDWTDQVSLFQPRQAAF
                     WLFSLLLLISAFGFVEEQLAFANASTTAWLVGLALLAAYAVPVYVLVNKLDLFEREPT
                     SLLIGALLWGAIIATFLAARINGEWAGIVQKLFGAEFAREWGAALIGPGVEELVKFLG
                     VIVLYLIARKEFDDVLDGFVYGALVGLGFTLEEDMYYFFTHFVGQAGASDLSGLFDGF
                     FMRIIVGGPYSHVLLTGLTGMGLAYYVTRPEIAKARRLLVAVGLYAAGVAAHFVWNSP
                     LLDSFLGSDPDNLTWITWAAIKGLPFLILLGLVVWLAIRREKRWVRETLADDVAAGVL
                     TDDEVDVLGDLRRRLDTRVAMKKRKGPAGDRLLTRLQHAQIGLALAESSTAPDRDLRV
                     GAARTLVRDLRAQLEALPDA"
     gene            complement(3682..4302)
                     /locus_tag="VIF63_05435"
     CDS             complement(3682..4302)
                     /locus_tag="VIF63_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238858.1"
                     /translation="MDPAPSAEQPRAAPTPALAATTRRGLDPGTRRQTYVVAAVIALL
                     FYGSQILNEALPANAADPAPGTPVSIGETTQITPLEGWSVTAHDNGFGVRLEKGIVVV
                     DLYQEAVGEDARELAAAYLDEVLRPDSSQFTSSEIEVVTASNGTAARFNYQGFFTGVE
                     FAIEGEVTAIFVDGTGIVADAWSRQGDLGDLLGEVHDMLASVEFAS"
     gene            complement(4405..4857)
                     /locus_tag="VIF63_05440"
     CDS             complement(4405..4857)
                     /locus_tag="VIF63_05440"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY8238859.1"
                     /translation="MPAARGALSRHPVVLRLDADRAADLQLRIADRITAFAGSMPFVY
                     LHAFAFGAWMLLFEKGPWPTLTLVVSLEAIFLSTFVMIGQNRQAAFQQAKADHEFREQ
                     EQELKKNTELTRAVHRLTSEVHEQIVGRDPSAAKGLDAEVTTGVDLQT"
     gene            complement(4970..5536)
                     /locus_tag="VIF63_05445"
     CDS             complement(4970..5536)
                     /locus_tag="VIF63_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEY8238860.1"
                     /translation="MLKLISGDWWIFALRGVAAILFGVLAFASPALTLATLVLMFGAY
                     VFVDGVTLLSALVRGEAGARRHAWTVGIMGVLGIVASIVTFVSPGITALSLLYVVAFW
                     AITMGVFQVVAAIALRREIEGEFWMGLGGVMSVAFGSLLIVSPGAGLVTLVWLLGAWA
                     IVFGASSLGLAYRLHGLGATQRQPAAAS"
BASE COUNT          918 a         2069 c         2099 g          811 t
ORIGIN      
        1 cgcgccgagg ccgccgtgat ccgcttcgag gcgacgcgcg tctactcgcg cgcctggacg
       61 cccgacgcgg cacgccggct ggaagccctt cgcatccgcg ccggacgcat cgtcaacacc
      121 agcccgcgaa cccggtacga cgcggtcctc gcgaccctcg gccgggaggt ggaagccgag
      181 cgccggtcgg tccacgtgca gcgccgaacg gtcgacctcg accgcgagcg cccgccggtc
      241 gacctggaac gccgacccgc ctgattgcat tcagcccggc cgagacaggc tcgggctagg
      301 cttccggcgt ggacgcagaa gcggaggcga caccgctcga tcccggcctg accggccagt
      361 tcgcgcgtcc cgttccgggc gtggacaacg gctacctcgc cggctatcgc gtccgcttcg
      421 acgaggctgc gggcgatggg ctgatgcgga cgtccgcgct catgcgctat gcccaggaca
      481 tcgcctggcg ccactccgag cagctcggct tcgatcgcga ctggtacgcg gagcgcggcc
      541 tgggctgggt cgtgcgcggc gtcgagctcg agctgcacgc gcccgtcccg atgggccaca
      601 cccttcgcct gaccacggca gtcgtcggcc atcgccggat ctgggcgcgc cggttgggcg
      661 agtgccggct ggccgatggg cgactcgccg cgcgcgtcac caccgactgg gtgctgctcg
      721 acggccgaaa ccggatcgtc cgcatccccg ccgatttcgg cgtcgcgttc cagaaccccg
      781 agctggatag cgagatcatt cgcgttgccg cgccgcagga ggagcccgcg cacacgctcg
      841 cgctcgcggt cggcccgcgc gatctcgatc cgctcgccca cgtcaacaac gccgtctacg
      901 tcgactggct cgacgaggcg ctcgagaccg ccggctggtc cggaaccgcc cgacccgagg
      961 gcagcacgct ccgtctcgag tacctcgcct ccgcggagcg cggcaaccaa gtggccgtcg
     1021 agctgtacgg cacggccgcc gactgggtcg cccgcatccg ccgcgccgac ggccaggagc
     1081 tcgttcgggc gagtggtgcg gccggagctg tcgacaccgc ggcggctccc cgagccgcat
     1141 ctcgggggtg acattccgcc gcgcacggcg gatcatcaac gcgtgacaac gctggtcgac
     1201 gagtacctcg ccaggacacc cggctcggcg gcgatgttcg aacgcgccgc gcggtcgctg
     1261 ccgggcggct cgacgcgaac gacgatctac agcgcgccgt atccgccgta catggttcgc
     1321 ggctcgggca tcagcacgtg ggacgtcgac ggcaacgagt accgcgactt cctgggcaac
     1381 tacacctcgc tgatcctggg tcacgcccat cccgaggtgg ttgccgcggt cgaggcgcag
     1441 gtccgccgcg gctcggcgtt cgccgcaccg accgagaccg agatcgagct ggccgaggag
     1501 atccgacggc gtctgccgtc gatcgaggcg atccgcttca cgaactccgg caccgaggcg
     1561 acgatgttcg ccctgcgcgc ggcgcgcgcc ttcacgaacc ggccgctgat cgccaagttc
     1621 gagctcgcct atcacggcac ccacgacacc gcgggtgccg gcacgccggg cgtgccccgg
     1681 gcgacctccg acctggtcgt cgagcttccg tggggggatc cggagggcgt cgagcgcgcg
     1741 ctcgcgggtc gcgagccgga ggtcgccgcg atcatcatcg agccggtcca gggcgccggc
     1801 ggtgtccgcg ccgcgacgcc cgagttcctc aacttcctgc gtgcgctcac aatccgcatc
     1861 ggggcgctgc tgatcctcga cgaggtcatc gcgttccgga tcggcccgaa cggggcgcag
     1921 ggcaggctcg gtggcatccg acccgacctc acgacgctcg gcaagatcat cggcggcggc
     1981 tatccgtcgg gcgcgttcgg cggtcgcacc gacgtgatgg cccagttcga cgcccgccga
     2041 tccggcgctt tggtccacgg cggcacgttc aacggcaacc cggtgtcggc ctcggcgggc
     2101 ctcgcgacgc tccgccattt gacgccggag cgctacgtcg agctcgatcg cctcggggat
     2161 cgactgcggg cggcgatcgc ggacggcgtg gcgcgcgccg gcctcgacgc gagcggtggc
     2221 ggcgtcgcct cgatcttcca ggtcgtcccc ggctcgagcc tcgttccggc tgatggcctc
     2281 agcccgcagg cggcgctgtt catcggcctc ttgctcgacg gcttccacct cgccccgcgc
     2341 ggcatgggcg cgctgtcgac ggtcacgacg gacgccgacg tcgacgacct cgcgcaggcg
     2401 gtggttgctc ggctcggcgc catgcaggcg gtcgccgcgg cctgagtcag gcgtccggca
     2461 gggcctcgag ctgggcgcgc aggtcgcgaa ccagcgtccg cgccgcgccg acgcgcagat
     2521 cccgatcggg cgccgtgctc gactcggcca gggccaggcc gatctgggcg tgctgcagcc
     2581 gggtcagcaa tcgatcaccg gccgggccct tgcgcttctt catggcgacg cgcgtgtcca
     2641 gccgccgccg gagatcgccg agcacatcga cctcatcgtc ggtgagcacg ccggcggcga
     2701 cgtcgtcggc gagggtctcg cgcacccacc gcttctcgcg gcggatcgcc agccagacga
     2761 cgaggccgag caggatgagg aacggcaggc ccttgatggc cgcccaggtg atccaggtca
     2821 ggttgtcggg atcggagccg aggaagctgt cgaggagggg cgagttccac acgaagtggg
     2881 cggccacgcc cgcggcgtac agcccgaccg caacgagcaa gcgccgcgcc ttcgcgatct
     2941 ccggccgggt gacgtagtag gcgaggccca tgcccgtgag gccggtcagg aggacgtgcg
     3001 aatacggccc gccgacgatg atccgcatga agaagccgtc gaagaggccg ctgaggtcgg
     3061 acgccccggc ctggcccacg aagtgggtga agaagtagta catgtcctcc tcgagcgtga
     3121 acccgaggcc gacgagcgcg ccatagacga agccgtcgag gacgtcgtcg aactctttgc
     3181 gcgcgatcag gtacaggacg atgacgccga ggaacttgac cagctcctcg acgccggggc
     3241 cgatcagcgc cgcgccccac tcccgcgcga actcggcgcc gaacagcttc tggacgatgc
     3301 ccgcccactc gccgttgatc cgcgctgcca ggaacgtggc gatgatcgcg ccccagagga
     3361 gcgcgccgat gagcagcgat gtcggctccc gttcgaacag gtcgagcttg ttgacgagga
     3421 cgtagaccgg cacggcgtag gcggcgagca gggcgagccc gacgagccag gccgtggtcg
     3481 acgcgttcgc gaacgcgagc tgttcctcga cgaagccgaa ggcggagatc agcagcagca
     3541 gcgagaacag ccagaacgcg gcctgccgcg gctggaacaa ggagacctgg tcggtccagt
     3601 cgctcggacc gcccgaccgc gcgggcggcg gtgtcgcgac tgtggtcggg ctgggtgcct
     3661 gcacgggcgt cgcggcgtcg gtcacgacgc gaactccaca gatgcgagca tgtcgtggac
     3721 ctcgccgagc aggtcgccga gatcgccctg gcgtgaccac gcatccgcga cgatgccggt
     3781 cccatcgacg aagatcgccg tgacctcgcc ctcgatcgcg aactcgacgc cggtgaagaa
     3841 gccctggtag ttgaatcggg ccgccgtgcc gttcgacgcc gtgacgacct cgatctcgga
     3901 cgacgtgaat tgcgacgagt ccggacgcag gacttcgtcg agataggccg ccgccagctc
     3961 gcgggcatcc tcgcccaccg cctcctggta caggtcgacg acgacgatgc ccttctcgag
     4021 ccggacgccg aagccgttgt cgtgggccgt gacggaccag ccctcgagcg gggtgatctg
     4081 cgtggtttcg ccgatcgaaa cgggcgtgcc gggcgcggga tcagcggcgt tcgcgggcag
     4141 cgcctcgttc aggatctggc tgccgtagaa gagcaacgcg atcactgcgg ccacaacgta
     4201 ggtctggcgg cgcgtgcccg ggtcgagacc gcggcgcgtc gtcgctgcga gcgccggggt
     4261 cggcgcagcc ctgggctgtt cagcgctcgg tgccggatcc acggtcgcgg atgatgacac
     4321 gctgagcccc ggcgccggtg gtccagctgg gtcggaaggc cggcacgatc gccggtgccg
     4381 acttgtatgg acgccgaccc gggctcaggt ctggaggtct acgccggtcg tcacttcggc
     4441 gtcgaggccc ttggccgcgg acgggtcccg cccgacgatc tgctcgtgga cctcgctcgt
     4501 cagtcggtgg accgcgcgcg tgagctcggt gttcttcttg agctcctgct cctgctcccg
     4561 gaactcgtgg tccgccttgg cctgctggaa cgccgcctgg cggttctggc cgatcatcac
     4621 gaaggtcgag aggaagatcg cttccagtga caccacgagc gtgagcgtcg gccaagggcc
     4681 cttctcgaaa aggagcatcc aggcgccgaa cgcaaaggcg tggaggtaga caaacggcat
     4741 cgagcccgcg aatgcggtga tgcgatcggc gatgcgcagc tggaggtcgg cggcgcgatc
     4801 cgcgtccagt cggaggacga ccgggtggcg cgacagcgcc ccccgcgcgg ccggcaccag
     4861 gtgagccgtc ggcagagcgg ggagcgagcc ggcgcggcgg ggcgaccaga gccgccgccg
     4921 ccgcgtggat tgttgcgtcg agggccgacg cggcccgtcc atgttccggt cagctcgcgg
     4981 cggccggctg gcgctgcgtc gcgccgaggc cgtggaggcg atatgcgagc ccgaggctcg
     5041 atgcgccgaa cacgatcgcc caggcgccga gcagccagac gagcgtgacg aggccggcac
     5101 ctggcgagac gatgagcagg ctgccgaagg caaccgacat cacgccaccg aggcccatcc
     5161 agaactcgcc ctcgatctcg cgtcgcagcg cgatagcggc aacgacctga aacacgccca
     5221 tcgtgatcgc ccagaaggcg acgacgtaga gcaacgacag ggccgtgatg ccgggcgaga
     5281 cgaacgtcac gatcgaggcg acgatgccga gcacgcccat gatgccgacg gtccatgcgt
     5341 gccggcgagc gccggcttcg ccgcggacga gcgcgctgag gagcgtgacg ccatcgacga
     5401 acacgtacgc gccgaacatg aggacaaggg ttgccagggt gagtgcgggc gaggcgaacg
     5461 ccaggacccc gaacaggatc gcggcgacac cgcgcagggc gaagatccac cagtccccag
     5521 agatgagctt gagcatcgaa gttctccttc tttctttgga acgcaatctc gcgtttctgg
     5581 cctcagcctc gacctcgacc ggatgagcga gcgtcacccg atccggtcga tttgcgtcga
     5641 ccggccgcgc gctggacccc ggtcgccgcc cgcgatgccg accgggaacc cgcccaacgg
     5701 cgcggcaggt ccagcctccg agaacggccg agacgccgag gagcgaggct catcggcgtc
     5761 gaggtggcct cccaggcaag cttcaggtag gaccgcccgg attcgagaaa acgaggcgaa
     5821 gccacggatg atttcgtcat gccgtggctc cgccgcgagt gaaggggttg gcgggtcgat
     5881 cagacccgaa tggcctc
//
LOCUS       DATBSW010000195         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_335322, whole genome shotgun sequence.
ACCESSION   DATBSW010000195 DATBSW010000000
VERSION     DATBSW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335322"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VIF63_05450"
     CDS             complement(<1..1506)
                     /locus_tag="VIF63_05450"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY8238861.1"
                     /translation="MRLSELFFTSLRDDPGEAEMPSHRLLLRAGYLRQLGSGIYSLLP
                     LGKKVSDRVEQIIREEINAIGGQEMEMPVVHPAEVWKESGRYQKIGPELVRFKDRGER
                     DMVLAMTHEEVVAILLRDIVQSYRQLPMIVYHFQTKFRDEPRSRGGLIRVREFVMKDS
                     YSCDLDDAGLDVSYRKHWAAYDAIFRRLGLDAIVVGADVGIMGGTGAHEFMVVNEFGE
                     DTLVLCDNCGFADNQQIAIIGKPDPESEDALPMEDVETPDATTIEALANFLGIPKSKT
                     AKAAFFVTGDDRFIVAIVRGDYDVNETKLVNTVKARRGLRPATVEEIKARGMEAGYGS
                     PLGARDSIVVVDELVVRSPNLVAGANRVGWHVKNVNVPRDYEPTHIAEITNARAGDPC
                     PKCGSPVRLAKGIEVGNIFKLGTDFTNAFGSMYLGEDGERHPIVMGSYGIGLGRNVAC
                     IVEAHHDEKGIAWPDEVAPYAAHLVSIGAGRDAAVLETADRLHALGNDSGHE"
     gene            1585..2589
                     /locus_tag="VIF63_05455"
     CDS             1585..2589
                     /locus_tag="VIF63_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY8238862.1"
                     /translation="MPDGFGALLVLVLVLAVGFDYINGFHDTANAIATSVSTRALRPT
                     HAILMSATANFVGALTGTAVAKTIASGIATTPPGDEGQIVVAAALVGAIAWNLITWRL
                     GIPSSSSHALIGGLIGAAIAASGVTSLNGPGIVDKVLFPLVASPILGVVLGFSLMVLL
                     LNVFRRAHPKRINDRFRRLQVISAAYMAFSHGSNDAQKTMGIMTLALVTGGILKDPTV
                     PLPVILLAATAISLGTAAGGWRIIKTMGQKVVKLDPVHGFAAETTAATIIIGASHFGM
                     PVSTTHVISSAIIGVGSSDRFSAVRWGVAGNIVIAWVLTIPASATVAWLTWELISRVL
                     "
     gene            complement(2573..3007)
                     /locus_tag="VIF63_05460"
     CDS             complement(2573..3007)
                     /locus_tag="VIF63_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238863.1"
                     /translation="MAAMLHTTEAPTGGIGHATNAPAAVPTRREGRPATAPGDAAREL
                     DTEVTRLLRVRGGELAEPLLWQRPSHIRAVDEIVVQLAPIRSRLGLLSSWAREAHRDP
                     QTRLAYAIVWLRLAQRRAGEYGPHRSAARHAVVQHRLITARG"
     gene            complement(3080..3769)
                     /locus_tag="VIF63_05465"
     CDS             complement(3080..3769)
                     /locus_tag="VIF63_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8238864.1"
                     /translation="MARTILVVEDEPTLRETLAEALEADGYEVVQAADGRAALARFRA
                     DHPDLVLLDLMLPELSGVEVTRQIRAESSVPIVMLTARDAEVDKVVGLELGADDYITK
                     PFSFRELSARLRAVLRRGEPTGTVLPPLVELGSVKVDLAGHRVLRDGAEVPMKPKVFD
                     LLAFLLRHPGQVITRDQLLEQVWGYDYPGETRTVDVHVHWLRSAVEEDPGHPALIGTV
                     RGTGYVLRRPA"
     gene            complement(3841..4251)
                     /locus_tag="VIF63_05470"
     CDS             complement(3841..4251)
                     /locus_tag="VIF63_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8238865.1"
                     /translation="MPHHITHEHVQAWLDAYEHAWQTYDAAEIGALFTEDAEYRWHPA
                     DEPEQGRDAIVFGWLNPNNDPSNRDAPGTYEGDYKPYAVDGSRAVAVGTSTYWTDASR
                     STVARVYYNNWLLEFGDDGKCCSFTEYWILPRGS"
     gene            complement(4251..>4575)
                     /locus_tag="VIF63_05475"
     CDS             complement(4251..>4575)
                     /locus_tag="VIF63_05475"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238866.1"
                     /translation="RLVVDPATLVPKRWDAATAAEALVAARATIADVGPVAYEADELE
                     PPLRALAEARGWKAGDLFMAIRVAVTGRTATPPLFDSLVALGPERTLARLDAAIDTVA
                     RLEDA"
BASE COUNT          661 a         1563 c         1609 g          742 t
ORIGIN      
        1 ctcgtggccg gagtcatttc ccagggcgtg cagccggtcg gcggtctcga gcacggcggc
       61 gtcacgcccg gcgccgatcg acacgagatg cgcggcatac ggcgcgacct cgtccggcca
      121 ggcaatcccc ttctcgtcgt ggtgcgcctc gacgatgcac gccacgttgc gcccgagccc
      181 gatcccgtac gagcccatga cgatcgggtg gcgctctcca tcctcgccga ggtacatcga
      241 cccgaacgcg ttcgtgaagt ccgtgccgag cttgaagatg ttgccgacct cgatgccctt
      301 cgccaaccgt acgggcgagc cgcacttcgg gcagggatcg cccgcgcggg cgttcgtgat
      361 ctcggcgatg tgggtgggtt cgtagtcgcg tggcacgttg acgttcttga cgtgccagcc
      421 gacgcgattc gcgccggcaa cgaggttcgg cgatcgaacg accagctcgt cgacgaccac
      481 gatcgaatcg cgcgcgccga gcggtgagcc gtagccggcc tccatgcccc gtgccttgat
      541 ctcctcgacc gtggccggcc gcaggccgcg acgggccttc acggtgttga ccagcttcgt
      601 ctcgttcacg tcgtagtcgc cgcgcacgat cgcgacgatg aagcggtcgt cgccggtgac
      661 gaagaacgcg gccttggcgg tcttcgactt ggggatgccc aggaagttgg cgagcgcctc
      721 gatcgtcgtc gcgtcgggcg tctcgacgtc ctccatcggg agggcgtcct cggactcggg
      781 gtcgggcttg ccgatgatcg cgatctgctg gttgtcggca aagccgcagt tgtcgcacag
      841 gacgagggtg tcctcgccga actcgttgac gaccatgaac tcgtgggcgc cggtgccgcc
      901 catgatcccg acgtccgcac cgacgacgat cgcgtccaga ccgagccgcc ggaagatcgc
      961 gtcgtacgcc gcccagtgct tgcggtagct gacgtcgagc cccgcgtcgt cgaggtcgca
     1021 cgagtacgag tctttcatca cgaactcgcg gacgcggatg aggccgccac gcgagcgagg
     1081 ctcgtcgcgg aacttcgtct ggaagtggta gacgatcatc ggcagctggc ggtagctctg
     1141 gacgatgtcg cggagcagga tcgcgacgac ctcctcgtgg gtcatcgcga ggaccatgtc
     1201 gcgctcgccg cggtccttga accggacgag ctccgggccg atcttctggt agcgcccgct
     1261 ctccttccag acctcggccg gatgcacgac gggcatctcc atctcctggc cgccgatcgc
     1321 gttgatctcc tctcggatga tctgctcgac ccggtcgctg accttcttgc cgaggggcag
     1381 cagcgagtag atgcccgacc cgagctggcg caggtagccg gcgcgcagca gcaaccgatg
     1441 cgacggcatc tcggcctcgc cgggatcgtc gcgcagggac gtgaagaaca gctcgctcag
     1501 tcgcatcgga acgcgaagga tacggacgcg cggccggcga cgcacgcaag gtccgaccgc
     1561 tgcggcgctg ctagccttcc gatcgtgccc gacggctttg gtgcgctcct ggtcctggtc
     1621 ctcgtgctgg ccgtcggctt cgactacatc aacggcttcc acgacacggc gaacgccatc
     1681 gcgacgtcgg tgtcgaccag ggcgctgcgc ccgacccacg cgatcctgat gtcggcgacc
     1741 gcgaacttcg tcggggcgct gaccggcacc gcggtcgcca agacgatcgc ctccggcatc
     1801 gccacgacgc cgcccggtga cgagggtcag atcgtcgtgg cggccgcgct cgtcggggcc
     1861 atcgcctgga acctgatcac ctggcggctc ggcatcccgt cgtcctcgag ccacgcgctg
     1921 atcggtgggc tgatcggagc ggcgatcgcc gcgtccggag tcacctccct gaacggcccg
     1981 ggcatcgtcg acaaggtcct cttcccgctc gtcgcctcgc cgattctcgg cgtcgtgctg
     2041 gggttcagcc tcatggtgct gctgctgaac gtgttccggc gcgcgcaccc gaaacggatc
     2101 aacgaccgat tccggcggct ccaggtcatc tccgcggcat acatggcctt cagccacggc
     2161 tcgaacgacg cgcagaagac gatgggcatc atgaccctcg cgctcgtcac gggcggcatc
     2221 ctcaaggacc cgaccgtgcc gctgcccgtg atcctgctcg ccgcgaccgc catctcgctc
     2281 gggaccgccg ccggcggctg gcggatcatc aagacgatgg gccagaaggt cgtcaagctc
     2341 gacccggtcc acggcttcgc cgccgagacg acggcggcca cgatcatcat cggcgcctcg
     2401 cacttcggca tgcccgtgtc gacgacgcac gtgatctcgt cggcgatcat cggcgtgggt
     2461 tcgagcgatc ggttctcggc ggtgcgctgg ggcgtcgccg gcaacatcgt catcgcctgg
     2521 gtcctgacga ttcccgcctc cgcgacggtg gcctggctta cctgggagct gatcagccgc
     2581 gtgctgtgat cagccggtgc tgcaccacgg cgtgacgcgc tgctgatcgg tgcggaccgt
     2641 actcgccggc ccgacgctgg gcgagtcgca gccagacgat cgcgtaggca agccgagtct
     2701 gcgggtcgcg atgggcttcc cgcgcccatg acgacagcag cccgaggcgc gagcggatcg
     2761 gtgcgagctg gacgacgatc tcgtcgaccg cgcgaatgtg gctcggacgc tgccagagga
     2821 gcggctcggc gagctcacca ccgcggaccc ggaggagtcg ggtgacctcg gtgtcgagct
     2881 cgcgagctgc gtccccgggc gcggtcgcgg gccgaccctc acggcgggtc ggcaccgcgg
     2941 ccggcgcgtt ggtggcgtgt ccgatcccgc ccgttggagc ttcggtcgtg tggagcatcg
     3001 ccgccatgcc cggatcgtcg aggccgaggg tcaacgtcgc gtgtgcgccg cgtgaacctt
     3061 cgcgctgatc gaggccgctt caggcgggcc ggcgaagcac gtagccggtg ccgcggaccg
     3121 tcccgatcag ggccggatgc ccggggtcct cctcgaccgc ggagcggagc cagtggacgt
     3181 gaacgtcgac cgttcgcgtc tcgccggggt agtcgtagcc ccatacctgc tcgagcagct
     3241 ggtcgcgcgt gatcacctgg cccggatggc gcagcaggaa cgcaaggagg tcgaagacct
     3301 tcggcttcat cggcacctcg gcgccgtcgc gcaggactcg atgaccggca agatcgacct
     3361 tcaccgagcc gagctcgacg agtggcggca ggacggttcc cgtcggctcg ccgcggcgaa
     3421 gcacggcacg aaggcgcgcc gacagctcgc ggaagctgaa cggtttcgtg atgtagtcgt
     3481 cggccccgag ctcgagcccg acgactttgt cgacctcggc atcgcgcgcc gtgagcatga
     3541 cgatcgggac cgacgattcg gcccggatct ggcgcgtcac ctcgacgccc gagagctcgg
     3601 gcagcatcag gtcgaggagc acgaggtccg gatggtcggc gcggaagcgg gccagcgctg
     3661 cgcggccatc ggccgcctgc acgacctcgt agccgtccgc ctcgagggcc tcggcgaggg
     3721 tctcgcgcag cgtcggctcg tcttcgacga ccaggatcgt gcgtgccatt cgatcccgat
     3781 catacggacg attgttaacg cggaattggc ccccggttgc cgcgaggtcc ggcgcgtgga
     3841 tcagctgccg cggggcagga tccagtactc ggtgaaggag cagcacttgc cgtcgtcgcc
     3901 gaactcgagc agccagttgt tgtagtagac gcgggcgaca gtcgaacgcg aggcgtcggt
     3961 ccagtacgtg ctggtgccga cggcgacggc gcggctaccg tcaacggcgt acggcttgta
     4021 gtcgccctcg tacgtcccgg gagcgtcgcg attcgacggg tcgttgttcg ggttgagcca
     4081 gccgaagacg atcgcgtcgc gtccctgctc gggctcgtcg gcgggatgcc agcggtactc
     4141 cgcgtcctcc gtgaagagcg cgccgatctc cgcggcgtcg taggtctgcc aggcgtgctc
     4201 gtaggcgtcg agccaggcct ggacgtgttc gtgggtgatg tggtgcggca tcaggcgtcc
     4261 tccaatcggg cgacggtgtc gatggcggcg tcgagccgcg cgagcgtgcg ttccgggccg
     4321 agcgcgacca gcgagtcgaa caggggtggc gtggcggtgc ggccggtgac cgcgacgcgg
     4381 atggccatga acaggtcgcc ggccttccag ccacgagcct ccgcaagcgc gcggagcggc
     4441 ggctccagct cgtcggcctc gtaggcgacc ggtccgacgt cggcgatcgt ggcgcgcgcg
     4501 gcaaccagcg cctcggcggc cgtggccgcg tcccagcgct tcgggacgag cgtcgccggg
     4561 tcgaccacga gccgg
//
LOCUS       DATBSW010000196         5508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_265216, whole genome shotgun sequence.
ACCESSION   DATBSW010000196 DATBSW010000000
VERSION     DATBSW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265216"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VIF63_05480"
     CDS             complement(<1..493)
                     /locus_tag="VIF63_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8238867.1"
                     /translation="MFRRILIANRGEIALRILRACRTLGVEAVVAYSEADRDSLPAQL
                     ADEAICIGPPDARRSYLSAPAVISAALVTGCDAIHPGYGFLSEDETFAEVVRAHDLTF
                     IGPPPDVLDRFASKEGTRRLLGGFGLPTIPGSTMISDDDHALREADRIGYPVLIKPSA
                     GGGG"
     gene            complement(493..1182)
                     /locus_tag="VIF63_05485"
     CDS             complement(493..1182)
                     /locus_tag="VIF63_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8238868.1"
                     /translation="MPDAGRQRERPDERSDDARIADHEGISRLTEDLLPALIAKLSTT
                     QLGELEVREGDWQVRLRRPYGVGPGEGRRSTDKASRGQPGHEGHGHGRAGVEGGRARG
                     AGGASGSGSGSGAAGAPSAGGGASSNGAGGGAARVGGPRDGETAADRMRTIATSPAVG
                     FFTPGTRAVSGTRVRAGDSLGSVDVLGVREDVVAPADGVVGSTLVEAGTAVEYGQELI
                     RIELTAPVEAR"
     gene            complement(1186..2208)
                     /locus_tag="VIF63_05490"
     CDS             complement(1186..2208)
                     /locus_tag="VIF63_05490"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY8238869.1"
                     /translation="MVDRLRGRIGEGKGSKADSRDIASLDPKDAMWRRVQLARNLGRP
                     RTLDLLAAMADEVIELHGDRGFGDDPAMVAGFARIDGRRIAFVGQQKGADTEENIRRN
                     FGMPHPEGYRKSMRVMELAERFGLPIVTFVDVPGAHPGPESEERGIAEAIARSIGLMS
                     RLRTPIVAIITGEGGSGGALAIAVGDVVIALENAVYSVISPEGCASILWRTAEEAPAA
                     ALAMKMSAQDQLELGVIDRVIPEPGEGAHADPRETARRLRTVIVGELDRLAALTPDEL
                     VEARYRRYRALGPYTEVSTPAVAPPPSRGLADRLRDLLDPTRWSMGTPVPGSSRRDEP
                     STNEEV"
     gene            complement(2201..3256)
                     /gene="accD"
                     /locus_tag="VIF63_05495"
     CDS             complement(2201..3256)
                     /gene="accD"
                     /locus_tag="VIF63_05495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY8238870.1"
                     /translation="MPIKLPSFRPRRDVYPPDLWTKCPSCGEMLFNKQLEKNDRICPT
                     CEHHFRISAMARLEMLLDPGTFQERDAGLQSLDPLGFVDQKAYPDRIATAQSQTGLRD
                     AAVRGIGAIEGMAVSICVMDFGFMGGSMGAVVGEKVTRAAEDALAERLPLIIVSASGG
                     ARMQEGTLALMQLAKTCAILERVRASRVPYVSVMSDPTTGGVFASYAVLGDVNLAEPN
                     ALIGFAGARVSAGTIAQELPAGFQRSEFLFEHGFIDRVITRRALRGEIASLLRFMRVP
                     ELATVDSRSGFSPVGAFVSSMVGVASGVAGVATGVATGVASAVTGSGSTPTNGSAGAN
                     GNGTAVAAPDAEGARDG"
     gene            complement(3260..3757)
                     /locus_tag="VIF63_05500"
     CDS             complement(3260..3757)
                     /locus_tag="VIF63_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238871.1"
                     /translation="MSLHRRDGGEIEALITDRYLDSLMAVNRRDEDGILGDPRSSLGA
                     AVRAVSRRLSNDLPRFHPSFRFEERLALRLAEVAASMRLPVAAGGESQPVAIRSIAAA
                     RPDPLEVVDEGIDGHPDSRPLLIGGAVAASALSLAGAWLAWRRRTSPMARAVRAAHAM
                     RQPGA"
     gene            complement(3754..4392)
                     /locus_tag="VIF63_05505"
     CDS             complement(3754..4392)
                     /locus_tag="VIF63_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8238872.1"
                     /translation="MGAAKDAVPRIAHLDQDRPLVVAAQRDPARFDALYRRYLARVYS
                     FAYYELGDHHAAEDATEATFVAALTNLSRFQERARPADGEGASTFRVWLFQIARNEVA
                     GERRRARRRPSEPIGETIIADPLNVEDEAVRRDQARAAWRAVGRLPGERRQAMVMRFV
                     DELSTAEIAGVLGRSEGAVRVLIHRALKSVAGELGKAEKRADTGSGERGRTS"
     gene            complement(4421..5257)
                     /locus_tag="VIF63_05510"
     CDS             complement(4421..5257)
                     /locus_tag="VIF63_05510"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY8238873.1"
                     /translation="MTARTFPARLERFDAVGSTNDVVAGWLAEGTPELCVAVADEQTA
                     GRGRNGRTWTAPRDAALLCSLGFRPTYLDPAQQWQLAAIVSLAMADAVEVVSESVAGT
                     VRLKWPNDLVVAQSDAVRKLAGVLGETVGAGTDDPRAVIGIGINAGWQRHDFPPELAQ
                     SMTSVVELCLARDGAGDANRALTPAQLRDELLKTFLRSLRPRLTDLHAGRFPAGEWRG
                     RQLTNGEVVRLERPDGTAELVRAIDVDADSGALMVDSLSGEWPARAITVGEISHVRLA
                     GV"
BASE COUNT          783 a         2004 c         1967 g          754 t
ORIGIN      
        1 tgccgccgcc gcccgcagac ggcttgatca gcaccgggta gccgatccga tcggcctcgc
       61 gcagggcgtg gtcgtcgtcg ctgatcatcg tcgagcccgg gatcgtcggc aggccgaagc
      121 cgccgagcag gcgtcgggtc ccctccttcg aggcgaaccg atcgagcacg tcgggcggcg
      181 gcccgatgaa ggtcaggtcg tgggcgcgca ccacctcggc aaacgtctcg tcctccgaga
      241 ggaagccgta gcccgggtgg atcgcgtcgc aacccgtgac gagcgccgcg gagatgaccg
      301 ccggcgccga gaggtacgag cgccgcgcgt cgggcgggcc gatgcagatc gcctcgtcgg
      361 cgagctgcgc gggcaggctg tcgcgatcgg cctcgctgta cgcgacgacc gcctcgacgc
      421 cgagcgtccg gcaggcgcgc aggatccgca gtgcgatctc gccgcggttc gcgatcagga
      481 tgcggcggaa catcagcgag cctcgactgg ggcggtgagc tcgatccgaa tgagctcctg
      541 gccgtactcg acggccgtgc cggcctcgac cagggtcgaa ccgacgacgc cgtctgccgg
      601 cgcgacgacg tcctcgcgga cgccgagcac gtcgaccgag ccgaggctgt cgccggcgcg
      661 cacgcgcgtg ccggacacgg cccgcgtgcc cggggtgaag aagccgaccg caggtgacgt
      721 tgcgatggtc cgcatccgat cggcggcggt ctcaccgtcg cggggtccgc ccacacgggc
      781 agcgccaccg ccggcaccgt tcgacgaagc gcccccgccc gcgctcggag cacccgctgc
      841 acccgagcca gagcctgatc cggatgcgcc gccggccccg cgagcccgtc cgccctcgac
      901 gcctgcgcgg ccgtggccgt ggccttcatg gccgggttgc ccgcgcgatg ccttgtcggt
      961 cgaacggcgg ccttcgccgg ggccgacgcc gtatggccgc cggagccgga cctgccaatc
     1021 gccctcgcgg acctcgagct cgccgagctg cgtcgtcgag agcttcgcga tgagcgccgg
     1081 caggaggtct tcggtcaggc gcgagatgcc ctcgtgatcg gcgatccggg cgtcgtcgga
     1141 tcgctcatcg gggcgttcgc gctggcggcc tgcgtcgggc accgctcaga cctcctcgtt
     1201 cgtcgacggc tcatctcgcc gcgacgagcc cggcacgggc gtgcccatcg accagcgggt
     1261 cgggtcgagc aggtcgcgca ggcgatcggc cagtccccga ctgggcggtg gcgccacggc
     1321 gggcgtggag acctcagtgt acgggccgag tgctcgatag cgccggtagc gcgcctcgac
     1381 cagctcatcg ggcgtgaggg cggccagtcg atcgagctcg ccgacgatca ccgtgcgcag
     1441 gcgccgggcg gtttcgcgtg gatccgcgtg ggcgccctcg ccaggttcgg ggatgacgcg
     1501 atcgatgacg ccgagctcca gctggtcctg ggccgacatc ttcatggcca gcgcggcggc
     1561 aggcgcctct tcggcggtgc gccacaggat cgaggcgcag ccctcggggc tgatcaccga
     1621 gtacacggcg ttctcgaggg cgatcacgac gtcgccgacc gcgatcgcga gcgccccgcc
     1681 ggagccgccc tcgccggtga tgatcgcgac gatcggcgtg cgcaggcgac tcatcagccc
     1741 gatcgagcgg gcgatcgcct ccgcgatgcc acgctcctcc gattcagggc cgggatgggc
     1801 gccgggcacg tcgacgaagg tcacgatcgg caggccgaag cgctccgcca gctccatcac
     1861 ccgcatcgac ttgcgatagc cctccgggtg gggcatgccg aagttgcgcc ggatgttctc
     1921 ctcggtgtcg gcgcccttct gctggccgac gaaggcgatg cgacggccgt cgatccgggc
     1981 gaagcccgcg accatcgccg ggtcgtcgcc gaagccgcgg tcgccgtgga gctcgatgac
     2041 ctcgtcggcc atcgcggcga gcaggtcgag ggtgcgcggg cgaccgaggt tgcgggcgag
     2101 ctggacacgc cgccacatgg cgtccttcgg gtcgagcgaa gcgatgtcgc gcgaatcggc
     2161 cttcgagccc ttgccctcac cgatgcggcc gcgcaggcgg tcaaccatca cgagcacctt
     2221 cggcatctgg agccgcaacc gcagtgccgt tgccgttcgc accggcgctg ccgttcgtcg
     2281 gggtcgagcc ggagcccgtc accgcgctgg cgacgccggt cgccacgccg gttgccacgc
     2341 ccgcgacgcc gctcgccacg ccgaccatcg acgacacgaa cgccccaacc gggctgaacc
     2401 cggaccggga atcgaccgtg gccagctcgg gcacgcgcat gaaccgcagc aggctcgcga
     2461 tctcgccgcg gagcgcgcgc cgggtgatga cgcggtcgat gaacccgtgc tcgaacagga
     2521 actcgctgcg ctggaagccc gcggggagct cctgggcgat cgtgcccgcg gagacgcgcg
     2581 cgcccgcaaa gccgatcagc gcgttgggct cggccaggtt cacgtcaccg aggaccgcgt
     2641 aggaggcgaa cacgccgccg gtcgtggggt cgctcatgac gctcacgtag ggcacccgcg
     2701 aggcgcggac gcgctcgagg atcgcgcacg tcttcgcgag ctgcatcagg gcaagcgtgc
     2761 cctcctgcat ccgcgcgccg ccggacgcgg acacgatgat gagcggcagt cgctcggcaa
     2821 gggcgtcctc ggccgcgcga gtgaccttct cgcccaccac cgcgcccatc gagccgccca
     2881 tgaacccgaa gtccatgacg cagatcgaga cggccatgcc ttcgatcgcc ccgatcccgc
     2941 gcacggcggc gtcgcgcagc cccgtctggc tctgggcggt cgcgattcgg tccggatagg
     3001 ccttctggtc gacgaagccg agcgggtcga gcgactggag gccggcgtcg cgctcctgga
     3061 acgtccccgg gtcgagcagc atctcgaggc gggccatcgc cgagatccgg aagtggtgct
     3121 cgcatgtcgg gcagatccgg tcattcttct cgagctgctt gttgaacagc atttcgccgc
     3181 acgacgggca cttcgtccag aggtccggcg ggtacacgtc gcggcggggg cggaacgagg
     3241 gcagcttgat cggcatcgct caggctcccg gctgccgcat cgcgtgcgcg gcccggaccg
     3301 cgcgggccat cggcgacgtg cggcgacgcc acgcgagcca cgcaccggcg aggctcaggg
     3361 cggatgccgc gacggcgccg ccgatcagca gggggcgcga gtcgggatgg ccgtcgatgc
     3421 cttcgtcgac cacctcgagc gggtccggcc gtgccgccgc gatgctccga atcgccacgg
     3481 gctggctctc gccgccggcc gcgacgggca ggcgcatcga tgccgcaacc tcggcgagac
     3541 ggagggcgag gcgctcctcg aaccggaacg aggggtggaa gcggggcaga tcgttggaga
     3601 gacggcgcga gacggcacgc accgccgcgc cgagactgga gcgagggtca ccgaggattc
     3661 cgtcctcgtc gcgtcgattc accgccatca acgagtccag gtagcgatcg gtgatgagcg
     3721 cctcgatctc gccgccgtcg cggcgatgca ggctcacgag gttcgaccgc gctcgccgct
     3781 gcccgtgtct gcgcgcttct ctgccttgcc gagctcgccc gccacgctct tcagcgcgcg
     3841 gtggatcagc acgcggaccg caccctccga ccggccgagc acgcccgcga tctccgcggt
     3901 gctcagctcg tcgacgaagc gcatgaccat cgcctgccgg cgctcgccgg gcagccgccc
     3961 gacggcgcgc cacgccgcgc gagcctggtc gcgtcggacg gcctcgtcct ccacgttcag
     4021 gggatcggca atgatcgtct cgccgatcgg ctcgctcggc cgccgtcgtg cccgacggcg
     4081 ctcgccggca acctcattcc gggcgatctg gaacagccag acgcggaagg tcgacgcgcc
     4141 ctcgccatcg gcgggacgcg cgcgctcctg gaaccgcgag aggttcgtca gggcggcgac
     4201 gaacgtcgcc tccgtggcgt cctctgcggc gtgatggtca ccgagctcgt agtaggcgaa
     4261 ggaatacacg cgggcgaggt acctgcgata cagggcatcg aagcgggccg gatcccgttg
     4321 cgcggccacg accagcggtc ggtcctggtc gagatgggcg atccggggga cggcgtcctt
     4381 cgccgcaccc actctccaca atgcccggcg cgccacttcg ttacaccccg gccagtcgaa
     4441 cgtggctgat ctcgcccacc gtgatggcgc gagcgggcca ctcgccggac agcgaatcga
     4501 ccatgagggc gccgctgtcg gcgtcgacgt cgatcgcccg gacgagctcc gcggtgccat
     4561 ccggccgctc gagccggacg acctcgccgt tcgtgagctg ccggccgcgc cactcccccg
     4621 ccgggaacct gcctgcgtgg aggtcggtca ggcggggtcg aaggctgcgg aggaacgtct
     4681 tcaggagctc atcgcggagc tgtgccgggg tgagagcgcg gtttgcgtcg ccagcgccgt
     4741 cgcgggccag acacagctcg acgaccgacg tcatcgactg ggcgagctcc ggcgggaaat
     4801 catgccgctg ccaaccggcg ttgatgccga tcccgatcac cgcgcgcgga tcgtccgtcc
     4861 cggcaccgac cgtctcgccg agcacgccgg cgagcttgcg gaccgcgtcc gactgcgcga
     4921 cgacgaggtc attcggccac ttgaggcgga ccgtccccgc cacgctctcg ctcaccacct
     4981 cgacggcatc ggccatggcc agcgacacga tcgcggcgag ctgccactgc tgggccgggt
     5041 cgagataggt cgggcggaag ccgagcgagc acaggagcgc ggcatcacgc ggcgccgtcc
     5101 acgtccgacc gtttcggccg cggccggcgg tctgctcgtc ggccacggcc acgcacagct
     5161 ccggcgtgcc ctccgccagc cagccggcga cgacatcgtt cgtcgacccg acggcatcga
     5221 accgctcgag ccgcgccgga aacgttcgcg ccgtcacggt ccccttctgc cggaatcccg
     5281 gcaaacgtcg cacgatctgg gccgtcacct cgatcggccc ggcattctac cagtggcatt
     5341 cccggcttct ggcagcgtcg caagcaggcc gtggacggca gacctgaagg gtctaggctc
     5401 cggccgaggc cggacgttct cgttcgacac cgggtccggc ggagattgag gaggctccca
     5461 tgccgcggct tgccggacgt cgtgctctcg tattcgtggc catcgcca
//
LOCUS       DATBSW010000197         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_970802, whole genome shotgun sequence.
ACCESSION   DATBSW010000197 DATBSW010000000
VERSION     DATBSW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970802"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VIF63_05515"
     CDS             <1..490
                     /locus_tag="VIF63_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8238874.1"
                     /translation="AQTRDTADRVRALRAMGVRISIDDFGTGYSSLGYLQAFELDELK
                     IDRSFVSTTDSVGNPRLISRAIVELGRALGLDIVVEGIESESQADWFGSIGCQYAQGF
                     YFAAPLTPSQTDVFLAGGSGARRVRLPAISSVLELAAVAAEPGASITPIERHIRRRAA
                     GE"
     gene            707..1348
                     /locus_tag="VIF63_05520"
     CDS             707..1348
                     /locus_tag="VIF63_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238875.1"
                     /translation="MITSAAALAIVFTTDQVLPATSEHQAQLRIWLGSRAAGFTALIL
                     LAFEVVIGLVLSHPTNKSTWRLSKRLFPWHEHVWLFTVAFVAVHVVSIATDRFANVGW
                     VGVFVPGMSEYRTVPVAIGTLGLYALLITGLTARVTKLLPAGWWLKLHRLSLVVLGLA
                     WAHGMLAGTDTFDAAAIYGGTFAAVAIAAVYRYWIVRSGRPTFATSLAREDHR"
     gene            1345..1800
                     /locus_tag="VIF63_05525"
     CDS             1345..1800
                     /locus_tag="VIF63_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238876.1"
                     /translation="MTRHIAFRRTLVTLGVVGTLLVAGLTIRTAASWASTQAPLGVAP
                     VSVESVQQALDSERARSDALAAQLRTLDSSTADLAAALDAAEAQVNADALTATDLRSA
                     LAAAQSKLAKLEASLKAASSRTTTTRTTTTRTTNTDSAEDREDEEEHDD"
     gene            1793..2224
                     /locus_tag="VIF63_05530"
     CDS             1793..2224
                     /locus_tag="VIF63_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238877.1"
                     /translation="MTDPRWFARLPSHIWVLLGASTAGYALLLAGVAGLQSQTEASLA
                     ASRQPAADGIVELAAGNDALLARLDAARADYARTAGVYTSAGGKLDALHGHVDALAAM
                     VAEIDGVSRSLPTSVKLPAVRSSVSSVKVPRTSGTTGASGG"
     gene            2235..3200
                     /locus_tag="VIF63_05535"
     CDS             2235..3200
                     /locus_tag="VIF63_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8238878.1"
                     /translation="MAISTAAVEMRREARALGSPLRLTVAGRADAAAVDLAWAAVIGT
                     FDEVDRAMSRFREDSDITKLNRARRPLAGLSRHLTAALVAADRANRMTGGRFDARVVG
                     ALESLGFAAVRQSLTSPIGPAPRAERPIQRFMRRDGRRGEIELIEPVDLGGIGKGLAL
                     RWAARRAAAELGPVGFLLEAGGDLVSRGHHDNAPWSIGIEDPLGGRDLVAVVALDREQ
                     AIATSSIRVGQRRAATGGVVHHLIDPRTGAPGGAGLVAVTVAWADPAWAEVWSKALFL
                     EGADAIGAAARRRGLAAWWVSESGQLSMTPAARQQTSWVRGEAVA"
     gene            complement(3197..>3702)
                     /locus_tag="VIF63_05540"
     CDS             complement(3197..>3702)
                     /locus_tag="VIF63_05540"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8238879.1"
                     /translation="IDAVARMTSDDLALQAPGTEHWPIWAICGHMAGTRVFWLCDVFG
                     EAGIEQTPWPNGTVGPGWEDDLSVPRSAEELVWALTSTWQVIEGLLARWTTDSMDVTA
                     QRIKAGRPPEVHTRQSILLRMITHEGYHVGEINIALGAAGREQIDPWPGREWQADAPR
                     SLREGGS"
BASE COUNT          535 a         1285 c         1293 g          589 t
ORIGIN      
        1 ggcccagacc cgggataccg ccgatcgcgt gcgggcattg cgggccatgg gcgtcaggat
       61 ctcgatcgac gacttcggga ccggctattc gtcgctcggc tacctgcagg cgttcgagct
      121 cgacgagctg aagatcgacc ggtcgttcgt ttccacgact gactcagtcg ggaacccgcg
      181 gctcatcagc cgggccatcg tcgagctggg ccgggcgctc gggctcgaca tcgtggtcga
      241 agggatcgag tcggagagcc aggccgactg gttcggctcg attggctgcc agtacgccca
      301 gggcttctac ttcgcggcgc cgctcacgcc gtcgcagacc gacgtgttcc tcgcaggcgg
      361 atccggcgcg cggcgcgtcc gcctccccgc gatctccagc gttctcgagc tggccgccgt
      421 ggcggccgag cccggcgcct cgatcacccc gatcgagcgc cacatccgaa ggcgcgccgc
      481 gggcgagtag cggccggtcc cggagcttcc ccgggttacc ttcggccggg ccatcggacg
      541 ccaatgaggc gattgcccgt gaaacctttt cgtcgttgac tgcctcagag ggatatcgac
      601 ggggtcgcac ggggatctcc acatgacaac ggcagctcaa cgcgcgcaat cagtgcctcc
      661 tacgcgctcg ccacgcacga cgcggctgtc tggcgcggca ctcaccgtga tcacgtccgc
      721 agctgccctc gcgatcgtgt tcaccaccga ccaggtgctg cccgcgacct ccgagcacca
      781 ggcccagctc cggatctggc tggggagtcg cgctgccgga ttcaccgcac tcatcctcct
      841 ggcattcgag gtcgtcatcg ggctcgtgtt gagccatccc acgaacaagt cgacctggcg
      901 actctcgaag cgactctttc cgtggcatga acacgtgtgg ctattcaccg tcgccttcgt
      961 ggccgtgcac gtcgtctcga tcgccacgga tcgattcgcg aacgttggct gggttggcgt
     1021 gttcgtcccg ggcatgtcgg agtatcggac ggtgcccgtc gcgatcggga cactcggtct
     1081 ctacgccctc ctgatcacgg ggctgaccgc tcgggtcacg aagctgctgc ccgccggctg
     1141 gtggctgaag ctgcaccgcc tgagcctggt cgtgctcggc ctcgcctggg cccacgggat
     1201 gctcgcgggg accgacacct tcgacgctgc ggcgatctac gggggcacat tcgcggcggt
     1261 cgccatcgct gcggtctatc gctactggat cgtgcgctcg ggacgcccga ccttcgccac
     1321 ttcgctcgca agggaggatc accgatgacc agacacatcg cattccgtcg gacgctcgtc
     1381 acgctgggcg tcgtcggcac gctgctcgtc gccggactca cgatccggac ggccgcctcg
     1441 tgggcatcga cgcaggcgcc gctcggcgtc gcgcccgtgt cggtcgagag cgtgcagcag
     1501 gcactcgatt cggagcgggc gcgttcggac gcgctcgctg ctcagctgcg gacgctggac
     1561 tcgtcaacgg cggacctcgc cgcagcgctc gacgcggccg aggcgcaggt gaacgccgac
     1621 gcgttgaccg caacggatct caggtcggcg ctcgctgcag cgcaatcgaa gctggcgaag
     1681 ctggaagcct cgctcaaggc ggcctcgagc cggacgacga cgacccggac gacgacgacc
     1741 cggacgacca acaccgactc ggccgaggat cgcgaggacg aggaggaaca cgatgactga
     1801 cccgcgctgg ttcgccaggc tgccgagtca catctgggtg ctgctcggcg cgtcgacagc
     1861 gggctatgcc ctcctgctcg ccggcgttgc cggcctccag tcgcagaccg aagcgtcctt
     1921 ggcggcgagt cggcaaccag cggctgacgg aatcgtcgag ctggctgccg gcaacgatgc
     1981 gctccttgct cgcctcgacg ccgcaagggc cgactacgca aggacggcgg gcgtgtacac
     2041 gagcgccggc gggaagctcg acgcgctcca cggtcacgtc gacgcgctgg ccgcgatggt
     2101 tgcggagatc gacggcgtca gccgttccct gccgacatcg gtgaagctcc cggccgtacg
     2161 cagctcggtg agctcggtga aggtcccgcg gacgagtggc acgacaggcg catccggggg
     2221 ctgagcgtcg gccgatggcg atctcgacgg ccgcagtcga gatgcgccgc gaggcacggg
     2281 cgctcggctc accgctccgg ctgacggtcg cgggacgtgc tgacgccgca gccgtcgatc
     2341 tcgcatgggc agcagtgatc ggcaccttcg acgaggttga tcgcgcgatg tcccgcttcc
     2401 gcgaggacag tgacatcacc aagctgaatc gggctcgacg gcctctggcc ggcctgtcgc
     2461 gacacctgac ggcggcgctc gtcgcggcgg accgggcgaa caggatgacg ggcggtcggt
     2521 tcgacgcgcg ggtcgtcggc gccctcgagt cgctcggctt cgcagccgtt cgacagtcgt
     2581 tgacgagccc aatcggtcca gcgccgcgcg cggaacgtcc gatccagcga ttcatgcgac
     2641 gagatggccg gcgtggtgag atcgagctca tcgagcccgt cgacctcggt ggcatcggaa
     2701 agggcctcgc tctgcggtgg gctgctcgac gcgccgcggc ggagctcggg ccggtgggct
     2761 tcctgctcga agccgggggc gatctggtct cccgcgggca ccacgacaac gcgccctggt
     2821 cgatcggcat cgaggatccg ctcgggggcc gggacctggt cgcagtggtc gccctggacc
     2881 gcgaacaggc gatcgccacc tcttcgatcc gggtcggcca gcggcgcgct gcaactggtg
     2941 gcgtcgtcca tcacctgatc gatccgcgaa ccggcgcacc gggtggcgct gggctcgtcg
     3001 ccgtcacggt cgcatgggca gacccggcgt gggcagaggt gtggtccaag gccctcttcc
     3061 tggagggcgc agatgcaatc ggcgcagctg cgcggcgacg tgggctggcg gcgtggtggg
     3121 tctcggaatc cggtcaactc tcgatgacgc cggcggcgcg ccaacagacg agctgggtcc
     3181 gcggcgaggc ggttgcctag ctgccaccct cacgtagcga gcgcggcgcg tctgcctgcc
     3241 actcgcgccc gggccacggg tcgatctgct cgcggccggc cgcgccgagt gcgatgttga
     3301 tctcgccgac gtggtagccc tcgtgcgtga tcatccgcag caggatcgac tggcgcgtgt
     3361 ggacctcggg aggcctcccg gccttgatgc gctgcgcggt cacgtccatc gaatcggtcg
     3421 tccagcgggc gaggagaccc tcgatgacct gccaggtcga ggtgagcgcc cagacgagct
     3481 cctcggcgct gcgcggcacc gagaggtcgt cctcccagcc ggggccgacc gtcccgttcg
     3541 gccatggcgt ctgctcgata ccggcctccc cgaatacatc gcatagccag aagacccgcg
     3601 tgccggccat gtgcccacag atcgcccaga tcggccagtg ctccgtgccc ggcgcctgga
     3661 gcgcgaggtc gtcggaggtc atccgcgcga ctgcgtcgat ga
//
LOCUS       DATBSW010000198         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1075694, whole genome shotgun sequence.
ACCESSION   DATBSW010000198 DATBSW010000000
VERSION     DATBSW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075694"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /gene="purB"
                     /locus_tag="VIF63_05545"
     CDS             <1..1360
                     /gene="purB"
                     /locus_tag="VIF63_05545"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8238880.1"
                     /translation="MIRRYTLAEMGSIWTEAARFEAMLRVELAVARAQAARGQIPAAA
                     LEAIETRSTIDVDRIAEIEKTTDHDIIAFVSQVAESIGPEGRYLHLGLTSSDVVDTGL
                     ALQLRAAGERLMADCDRLTLAVIGRARAEAETVMMGRTHSVHAEPTTLGLKFAGWAFE
                     VERGRRRLADAVDEIGTGKISGPVGTYSHLGPDVEAEVLAALGLHADPASSQIVQRDR
                     HAALLGAIAIVGGSLERFATEVRNLQHTEIGELQEPFTPGQKGSSAMPHKRNPILSER
                     IAGLARLLRGYAGTALEDQPLWHERDISHSSAERVILPDATIILDYMLVRMTGLVEGL
                     VVRSERMRENIARGLGLHASSRVLVTLVESGGLPREEAYAIVQRASLRAADERAPLRD
                     LLAVDPAVAAKLSLAQLDACFDESAFLQHVPTVIRRLDGLSDAVAARVSMPDPTVPDA
                     AG"
     gene            1347..2261
                     /locus_tag="VIF63_05550"
     CDS             1347..2261
                     /locus_tag="VIF63_05550"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8238881.1"
                     /translation="MLLAEHFVRSGKVRDLYDLPDGRMLLVASDRLSAFDVVLPTEIP
                     DKGRVLTGLSRFWFAETAGIVPNHLLSTDVASLPKAFAEHATELRGRMMIGRTANVLP
                     IEVIVRGYLAGSGWKEYRESGAVCGIALPDELREGDRLPAPIFTPSTKAPQGEHDQNV
                     PFSALVVVAGEETAEAVRAAALALYGHAAAITERAGILLADTKFEFGLAEVDGETRLL
                     LIDEALTPDSSRFWDAAAYRPGGPQPSFDKQYVRDWLEAQPWDKTAPGPALPDDIVEG
                     TRARYIEAYERITGASFERYLQEDVIAP"
     gene            2258..2548
                     /gene="purS"
                     /locus_tag="VIF63_05555"
     CDS             2258..2548
                     /gene="purS"
                     /locus_tag="VIF63_05555"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY8238882.1"
                     /translation="MTSYRFAVNVTPKPGILDPQGRATEGSLGHLGIEGVSAVRVGRR
                     VELTVESDDEGAARAIVERLASELLSNPLIEAYAIESIGAASSLVGVARGSD"
     gene            2548..3291
                     /gene="purQ"
                     /locus_tag="VIF63_05560"
     CDS             2548..3291
                     /gene="purQ"
                     /locus_tag="VIF63_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY8238883.1"
                     /translation="MGVRVGVIVFPGSNCDRDTVAALDLVGAEPVVLWHEQASLEGVA
                     AVVLPGGFAYGDYLRAGVIARFSPVMRAVAAFAADGGLVLGICNGFQVLAEAGLVPGA
                     LLRNQSLRFAGQEVRIAAQRSDTRFTNGLEVGRPLRMPVAHGEGCFYADDATLDALEH
                     DGQVLFRYVDAAGTVAGPEAAANPNGSLRAIAGVLNAAGNVAGLMPHPERASEAILGS
                     DDGIGIIRSLVESAARATSVAAPVGAGSR"
     gene            3309..>3577
                     /locus_tag="VIF63_05565"
     CDS             3309..>3577
                     /locus_tag="VIF63_05565"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238884.1"
                     /translation="MEPLHRRLGVTDEELDAIRDRLGGRDPNDLELAMFSVMWSEHCS
                     YKSSKPLLRTLPTAGEGVVAGPGENAGVISIGDGLAVAFKIESHN"
BASE COUNT          519 a         1249 c         1280 g          529 t
ORIGIN      
        1 catgatccgt cgctacacgc tggccgagat gggctcgatc tggaccgagg cggccaggtt
       61 cgaggcgatg ctgcgcgtcg aactggcggt ggccagggcc caggccgcgc ggggccagat
      121 cccggcggcc gcgctcgagg cgatcgagac gcgctcgacc atcgacgtgg atcggatcgc
      181 cgagatcgag aagaccaccg atcacgacat catcgccttc gtcagccagg tcgccgaatc
      241 gatcggaccg gagggtcgct acctccacct cgggctgaca agcagcgacg tcgtggacac
      301 cgggctggcg ctccagctcc gcgcggccgg ggagcggctc atggccgatt gcgatcgcct
      361 gaccctcgcc gtcatcggtc gtgcccgggc ggaggccgag accgtgatga tgggccgcac
      421 ccattcggtc cacgccgagc cgacgacgct cgggctcaag ttcgccggtt gggcgttcga
      481 agtcgagcgt gggcgaaggc ggctggccga cgcggtcgac gagatcggta ccggcaagat
      541 ctccgggccg gtcgggacct acagccacct cggtccggac gtcgaggccg aggtgctcgc
      601 cgcgcttggg ctccacgcgg acccggcaag ctcgcagatc gtccagcgcg accggcatgc
      661 cgcgctgctc ggcgcgatcg ccatcgtcgg cggctcactc gagcgcttcg cgaccgaggt
      721 gcgcaacctc cagcacaccg agatcggcga gctccaggaa ccgttcacgc cgggccagaa
      781 gggctcctcg gcgatgcccc acaagcgcaa tccgatcctc tcagagcgga tcgcggggct
      841 cgcccgcctg ctccgtggct atgcgggcac ggcactcgag gaccagccgc tctggcacga
      901 gcgcgacatc agccattcga gcgccgagcg ggtcatcctg cccgacgcga cgatcatcct
      961 cgactacatg ctcgtgcgga tgaccggtct ggtcgagggc ttggtggtcc ggtcggagcg
     1021 gatgcgcgag aacatcgccc gcgggctggg gctgcatgcg tcgtcgcgcg tgctcgtcac
     1081 cctggtggag agcggcggcc tgccgcgcga ggaggcctac gcgatcgtgc agcgagcgtc
     1141 gctccgggcc gccgacgagc gggcgccgct gcgggatctg ctggccgtgg accccgcagt
     1201 cgccgccaag ctctcgctcg cccagctgga cgcctgcttc gacgagtcgg ccttcctgca
     1261 gcacgtcccg acggtcatca gacgactcga cggcctgagt gatgccgtgg ccgcgcgcgt
     1321 ctccatgccc gatccgacgg tgcccgatgc tgctggctga gcacttcgtc cgttccggca
     1381 aggtgcgcga cctctacgac ctgcccgacg gccggatgct gctggtcgcg tccgaccgcc
     1441 tgtcggcgtt cgacgtcgtg ctcccgaccg agatcccgga caagggtcgg gtcctgaccg
     1501 gcctctcgag gttctggttc gcggagaccg ctggcatcgt gccgaaccat ctcctgtcga
     1561 ccgacgtcgc ctcgttgccg aaggccttcg ccgaacatgc gaccgagctc cgagggcgga
     1621 tgatgatcgg ccggaccgcg aacgtgctac cgatcgaggt catcgtccgc gggtacctcg
     1681 cggggagtgg ctggaaggag taccgggaga gcggcgccgt gtgcggcatc gcgctgcccg
     1741 acgagttgcg agaaggcgac cggctccccg cgccgatctt cacgccatcg accaaggcgc
     1801 cccagggtga acatgaccag aatgtgccct tctcggccct cgtggtcgtc gcaggcgagg
     1861 agacagccga ggcggtgcgc gccgcggccc tggcgctcta cgggcatgcc gcggccatca
     1921 ccgagcgggc cgggatcctg ctggccgaca cgaagttcga attcgggctc gcagaggtcg
     1981 acggcgagac ccgcctgctg ctgatcgacg aggccctgac gcccgattcg tcgcgcttct
     2041 gggatgccgc ggcctaccga ccgggcggtc cccaaccgag cttcgacaag cagtacgtgc
     2101 gcgactggct cgaagcccag ccgtgggaca agaccgcgcc cgggccggcg ttgccggacg
     2161 acatcgtcga gggtacgcga gcccgctaca tcgaggcata cgagcggatc accggcgcca
     2221 gcttcgagcg ctatctccag gaggatgtca tcgccccatg accagctatc ggttcgccgt
     2281 gaacgtgacc cccaagcccg gcatcctcga tccacagggt cgggccaccg agggcagcct
     2341 cggccatctc gggatcgagg gcgtgagcgc cgtccgcgtc gggcgccggg tcgagctgac
     2401 ggtcgagtcg gatgacgagg gcgcggcgcg cgccatcgtc gaacggctgg cctcggagct
     2461 gctgtcgaac ccgctcatcg aggcgtacgc catcgagtcg atcggcgccg cgtcgtcgct
     2521 ggtcggcgtg gccaggggat cggactgatg ggcgtccgcg tcggcgtgat cgtgttcccg
     2581 ggcagcaact gcgatcggga cacggtcgcc gcgctcgacc tggtcggggc ggaaccggtc
     2641 gtgctgtggc acgagcaggc gtcactcgag ggtgtggccg cggtggtctt gccgggtggc
     2701 ttcgcctatg gcgactacct ccgagcgggc gtcatcgccc ggttcagccc ggtcatgcgg
     2761 gccgtcgccg ccttcgcggc cgatggcggc ctagtcctgg gcatctgcaa cggcttccag
     2821 gtcctcgccg aggccgggct ggtgccgggc gcgctgctgc gcaaccagtc gctccggttc
     2881 gccggccagg aggtccgcat cgccgcgcaa cggtccgaca cccgcttcac gaacgggttg
     2941 gaggtcgggc gcccgctgcg catgccggtc gcccacggcg agggctgctt ttacgccgac
     3001 gacgcgacgc tcgatgccct ggaacacgac ggtcaggtcc tgttccggta tgtggacgcg
     3061 gcggggaccg tcgccggccc agaggctgcc gccaacccca acggctcgct ccgcgccatc
     3121 gcgggcgtgc tgaacgcggc cggcaacgtg gcgggcctga tgccccaccc ggaacgggcg
     3181 agcgaggcga tcctcgggtc ggacgacggg atcggtatca tccgctcgct cgtggagagc
     3241 gcggcccgcg ccacgtccgt ggctgcgccc gtgggtgccg gcagccgatg acgctcgcgg
     3301 agccgcccgt ggagccgttg caccgccggc tcggtgtcac cgacgaggag ctggacgcca
     3361 tccgcgaccg cctcggcgga cgggatccca acgacctcga gctcgcgatg ttcagcgtca
     3421 tgtggagcga gcactgctcg tacaagagct cgaagccgct gctgcggacc ctgccgaccg
     3481 ccggcgaggg cgtggtcgcc gggccgggcg agaacgccgg cgtcatctcg atcggcgatg
     3541 ggttggcggt cgcgttcaag atcgaatcgc acaacca
//
LOCUS       DATBSW010000199         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1146451, whole genome shotgun sequence.
ACCESSION   DATBSW010000199 DATBSW010000000
VERSION     DATBSW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146451"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VIF63_05570"
     CDS             complement(<1..666)
                     /locus_tag="VIF63_05570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8238885.1"
                     /translation="MLTIAPDRKALVTGAASGFGLGVVDRLLEAGARVVMADFSEALL
                     AGEAGRRGAAVHPVVMDVRIKASVQSGVAAAVEWLGGLDTLVQCAGVFDYGALGETTE
                     ESWDRQLDTNLKGTFLVAQAAMPHVVASARGRIVNIASEAGRKGWPLTSAYNASKFGV
                     VGLTQTWAREFGRNGVTVNCVCPAMTPDTRQGKEVTRQKLARTPGLTEAEVLRGYAEN
                     FPVG"
     gene            791..1228
                     /locus_tag="VIF63_05575"
     CDS             791..1228
                     /locus_tag="VIF63_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8238886.1"
                     /translation="MAATATGNMRFVTPNDVETMLLDWGTLKWLAESRVTGSGIAVGV
                     VVLEPGKGHLRHNHPGSEEVLYYISGEGRQMVEDDDGNEMYRDVKAGDAAYIPAGVYH
                     GTENTGWEPMRILAIYNPGGPEAIIRAGADCITFQPGEIAHRS"
     gene            1234..1671
                     /locus_tag="VIF63_05580"
     CDS             1234..1671
                     /locus_tag="VIF63_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8238887.1"
                     /translation="MHDEMLDADSRRNVWKRIEGDERPAWSDVTSTGTFQVVAGQTFD
                     RHHHDCDEYWLIFEGRAVIEIDEERYAIEAGDIVCTEIGREHDFLQVVEPVRGFWFEA
                     VLKPDGRAGHLHRSPERAAGHTVLGPNDHLAGHHAPHPPLDPA"
BASE COUNT          279 a          639 c          625 g          245 t
ORIGIN      
        1 gcccacgggg aagttctcgg cgtagccacg caggacctcc gcctcggtca ggcccggcgt
       61 ccgcgccagc ttctggcgcg tgacctcctt gccctggcgc gtgtcgggcg tcatcgccgg
      121 gcagacacaa ttgaccgtaa ctccgttgcg cccgaactcg cgcgcccacg tctgggtgag
      181 cccgacgacc ccgaacttcg acgcgttgta cgcggacgtg agcggccagc ccttgcgtcc
      241 cgcctccgag gcgatgttca cgatccggcc tcgagcgctc gccacgacgt ggggcattgc
      301 cgcctgggcg acgaggaacg tgcccttcag gttcgtgtcg agctggcggt cccagctctc
      361 ctcggtggtc tcgccgagcg cgccgtaatc gaacacgccg gcgcactgga cgagcgtgtc
      421 gagcccgccc agccactcga cagcggcggc gacgcccgac tggacggatg ccttgatccg
      481 gacgtccatc acgacggggt ggacggcggc accgcgccgg ccagcctccc ccgcgaggag
      541 cgcctcgctg aagtcggcca tcacgacgcg cgccccggcc tcgagcagcc gatcgacgac
      601 gcccaggccg aacccgctcg cggcgccggt gacgagcgcc ttgcggtcgg gcgcgatcgt
      661 gagcatgcgg acctccgtgg acgcgttcgg gagcattcat tatcggaccg ggcggcggct
      721 acagtgttgc ctcccgagcg cgcacgacgc gtgggacgaa cggtcggtcg atcggcgtgg
      781 aggtttggcg atggcagcga cggcaacggg aaacatgcgc ttcgtgacgc cgaacgacgt
      841 cgagacgatg ctcctcgact ggggcaccct caagtggctc gccgaatcgc gcgtgaccgg
      901 gtcgggcatc gccgtcggcg tcgtcgtgct ggagccgggc aagggccacc tgcgccacaa
      961 ccatccgggc tccgaggagg tcctctacta catcagcggc gagggccggc agatggtcga
     1021 ggacgacgac ggcaacgaga tgtaccgcga cgtgaaggcg ggcgacgccg cgtacatccc
     1081 ggcgggcgtc tatcacggca ccgagaacac gggctgggag ccgatgcgga tcctcgcgat
     1141 ctacaacccc ggtgggcccg aggcgatcat ccgcgccggc gcggactgca tcacgttcca
     1201 accgggcgaa atcgcccatc ggagctgagg gccatgcacg acgagatgct cgacgcggac
     1261 agccgccgca acgtctggaa gcgcatcgag ggcgacgagc gcccggcctg gtccgacgtg
     1321 acctccaccg gcacgttcca ggtcgtcgcc ggccagacgt tcgaccggca ccaccacgac
     1381 tgcgacgagt actggctgat cttcgagggc cgggccgtca tcgagatcga cgaggagcgc
     1441 tacgcgatcg aggcgggcga catcgtgtgc accgagatcg ggcgtgagca cgacttcctc
     1501 caggtggtcg agcccgtccg gggcttctgg ttcgaggccg tgctgaagcc ggatggccgc
     1561 gccggccacc tgcatcgatc gccggagcgc gccgccgggc acacggtgct cggcccgaac
     1621 gaccacctcg ccggccacca cgcgccccac ccgccgctcg acccggcctg accgaggacc
     1681 gaggccgacc gtgcagcggt tcatccacgt cggcaccggt ggctggggcc gcatgtggtc
     1741 gcggacgttc ctgccgcacg tgatccggct gggactcgcc gagcccgt
//
LOCUS       DATBSW010000200         7915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_124401, whole genome shotgun sequence.
ACCESSION   DATBSW010000200 DATBSW010000000
VERSION     DATBSW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124401"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VIF63_05585"
     CDS             <1..751
                     /locus_tag="VIF63_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8238888.1"
                     /translation="PVADALHVATLNIRNIADRWGERIPLLLADMAALQPDLIGLQEC
                     VFAVQQDRLLGAAGDGHYQTRRGWAGRPEYGNAILGKAPVTMSEGKRIDLGRNRSALR
                     VPVSLPSGAQLDYVVTHLHHLTADEAAREEQAQALTAWLAPIEGPLVVVGDFNAEPTE
                     STYQVMLDAGFRSSHVEANGAEPPVTWPSGIEAPGKDDDGDPGCLDYIWVRGPIAVDA
                     CTVEFNRPAVDDPTLYPSDHFGLSARIRVGG"
     gene            756..1433
                     /locus_tag="VIF63_05590"
     CDS             756..1433
                     /locus_tag="VIF63_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8238889.1"
                     /translation="MTTLRLAHRGDWRIAPENSLAAMQAALTVPGCDGLEFDIRSSFD
                     NVPVLLHDATLARVQRVPLSCSTLTAVQLAEHGVPTLGEVLAAVGCDPFLDVELKEPV
                     KGAIDALELERGGNDDKGQPELKNAVVSSFDAPVLGWLRSLRPTWPRWLNAYDLSPST
                     IATATDLGCTAISVEWHGLDADGVARATDSGLGVAAWTVREPADYLRLEAMGLLAVCA
                     EAAALEG"
     gene            complement(1458..1868)
                     /locus_tag="VIF63_05595"
     CDS             complement(1458..1868)
                     /locus_tag="VIF63_05595"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY8238890.1"
                     /translation="MIRVLFLCVHNSARSQMAEGMLRSWGSDRYEAASAGSVASEVRP
                     LAILAMGEIGIDIAGQTSKTSDVFAGQAFDYAITVCDDAREACPYFPGATTQLHWSFD
                     DPSAATGTDDERLAVFRRVRDEIGARIRTELLAR"
     gene            complement(1865..2809)
                     /locus_tag="VIF63_05600"
     CDS             complement(1865..2809)
                     /locus_tag="VIF63_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8238891.1"
                     /translation="MIGPGDRRNVLWLILAAACWGIGTVISKQAVAEMPPLTLLPIQL
                     ATSVGFLLVLTRVRGERMPTGREGRLLGRLGLLNPGLAYALSLVGLTQISASLAVLLW
                     ASEPIFILGLAALVLGERVGLPIIASSAVAILGLVLVVFDPAASGSSVGIALTVAGVV
                     VCAIYTVATRRWLLGSDSTFSVVLAQQVHALALAVGVVIALAATGQQMAPRTLTLGGL
                     ASAAASGLLYYAFAYSFYVTALRSVRASIAAASFYLIPVFGLAGGWLVGERLEPVQWL
                     GGIVVIAAVAIITTRIARPAAAAPARPVETLGSVESTT"
     gene            complement(2839..3759)
                     /gene="arsM"
                     /locus_tag="VIF63_05605"
     CDS             complement(2839..3759)
                     /gene="arsM"
                     /locus_tag="VIF63_05605"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEY8238892.1"
                     /translation="MSDQSLELNEIQQIVRDRYAETAIAVAAGLTKHEATHGTTAEAS
                     CCGPECCGPTVATSEVAVDGSARPVARVEQAAPQADACCGDGCCSDVSGTYGLELYDA
                     AERQSLPDAALLASLGCGNPTAVAELREGETVLDLGSGGGIDVLLSARRVGPTGRAIG
                     VDMTDEMLSLARRNAADAGATNVEFRKGTIEALPLDDASVDVVISNCVINLASDKSAV
                     FAEIARVLRPGGRVGVSDVVADDLLTAEQRAERGTYVGCIAGALSFREMRDGLRAVGL
                     TDVEVVPTHQIVEGMHSAIIRATKPVAVIA"
     gene            complement(3788..4012)
                     /locus_tag="VIF63_05610"
     CDS             complement(3788..4012)
                     /locus_tag="VIF63_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238893.1"
                     /translation="MFELYLSHTSGGEHSTDPRNQFHEIALRDARIASDWLSEVAPSV
                     VVAKPSLVSRLRLAFAGRPAPTTDACACPA"
     gene            4103..4468
                     /locus_tag="VIF63_05615"
     CDS             4103..4468
                     /locus_tag="VIF63_05615"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8238894.1"
                     /translation="MAKSTDPDIQLLAALADSTRMEILRELAGAAEVCACDFTDCCDV
                     SQPTISHHLKVLRDAGAVLSERHGNRVYYRIAPNLMERLGGIAGGLVPGGMVPASALA
                     VGRGSIASPIPLGSRATSN"
     gene            complement(4500..5303)
                     /locus_tag="VIF63_05620"
     CDS             complement(4500..5303)
                     /locus_tag="VIF63_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8238895.1"
                     /translation="MTDQGDTPAPKRKRRASGTTASIAKPAAAKRPSAKPASTKPAAA
                     KSAAAKAAPAKSPAKAAAPVKAPASPKRPPAKPTKPATETTATAPAAPEAEAAPMLVA
                     IPVPSQPLQLYLLRHADAGDLAAWPGDDADRPLSAKGRRQARRLGSLLSDLGFRPDVI
                     LTSPKLRAAQTARIVGRALGTKSTDDLRLAGPMDLADLGGLLAGHGDARRIVLVGHDP
                     DFSSIASSLSGAAIDLRKGALARIDLADGHPTAGAGSLRWLIPPGALPS"
     gene            complement(5300..5722)
                     /locus_tag="VIF63_05625"
     CDS             complement(5300..5722)
                     /locus_tag="VIF63_05625"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY8238896.1"
                     /translation="MRVIFVCTGNSARSQMAEAILRRDGGERFHVVSAGVSPRPVNPL
                     TIRSLAKVDIDISDAKSKPIGQFLGQRFDYVITVCDRARATCPVFPGGSETLHWGVDD
                     PAEATGTDAERQVAFDQALRELAVRIHTFLPLAVGHRR"
     gene            complement(5776..6471)
                     /gene="phoU"
                     /locus_tag="VIF63_05630"
     CDS             complement(5776..6471)
                     /gene="phoU"
                     /locus_tag="VIF63_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8238897.1"
                     /translation="METAAGAIRIDPTRSRAGLDRDERVVKDGILRMGSLVEAQIREA
                     VRSVAERDVEAADRVIAADERINEVQREIADAVVTTIATQSPVARDLRFLMGLDHVAF
                     ELERIGDHASSIAKQTRRLTEESVLRETRDLVEMGRLAADQVRGIVTALIDIDDDLAR
                     TVAARDDDIDHLYRTIFAEVIDLMRSDPRTVERGTRVILAAHWVERIGDRVTNIAEQV
                     VYLATGQIEDLNP"
     gene            complement(6482..7279)
                     /gene="pstB"
                     /locus_tag="VIF63_05635"
     CDS             complement(6482..7279)
                     /gene="pstB"
                     /locus_tag="VIF63_05635"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY8238898.1"
                     /translation="MQTADAAEAAAPDVTLELRNVSCYYGSFRAVRDVSLKVARYGVT
                     ALIGPSGCGKSTVLRTMNRMNDLVPSFHLEGSILLDDQDLYDKAVDPVAVRRRVGMVF
                     QKPNPFPKSIYENVAFGARINGFKGNTKDLVEGSLRRAALWDEVKDKLGQSALALSGG
                     QQQRLCIARAIATEPEIILMDEPCSALDPRATLQIEELMRELSGRYTIVIVTHNMQQA
                     ARASDLTAFLTMGEDRAGYLVEMDKTIKIFTNPREQLTEDYISGRFG"
     gene            complement(7393..>7915)
                     /locus_tag="VIF63_05640"
     CDS             complement(7393..>7915)
                     /locus_tag="VIF63_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8238899.1"
                     /translation="GVPSIIYGIFGLALFVRVLGLGRSPLAAALTLALLIMPIVIIAT
                     MEALRAVPDSQREGAYALGATRWQMVRRSVLPSAAPGILTGIILAMARAIGEAAPLIV
                     IGAASFVTFLPSPLKTGFTVLPIQVYDWASRPQEAFQGIAAAAILVLLLLMLVLNGLA
                     ILVRARLSRHLQW"
BASE COUNT         1210 a         2698 c         2805 g         1202 t
ORIGIN      
        1 gccggtggct gacgcgctgc acgtcgcgac gctcaacatc cgcaacatcg cggaccgctg
       61 gggcgagcgc atcccgctcc tcctcgccga catggccgcg ctccagccgg atctcatcgg
      121 gctccaggag tgcgtcttcg cggtccagca ggaccgcctg ctcggcgccg ccggtgacgg
      181 gcactaccag acgcgtcggg gatgggccgg gcggccggaa tacggcaacg cgatcctcgg
      241 caaggcccca gtcacgatga gcgagggcaa gcggatcgat ctcggtcgca accgatcggc
      301 gttgcgggtg ccggtcagcc tgccgtcggg cgcccagctc gactacgtcg tgacccacct
      361 ccatcacctc accgcggacg aggcggcgcg cgaggagcag gcacaggcgc tcacggcctg
      421 gctggcgccc atcgaaggcc cgctcgtggt cgttggcgac ttcaacgccg agccgaccga
      481 gtcgacctac caggtcatgc tcgacgccgg cttccgctcg tcgcacgtcg aggcgaacgg
      541 cgccgagccg cccgtcacct ggccctcggg catcgaggcg cccggcaagg acgacgacgg
      601 tgacccgggc tgcctcgact acatctgggt ccgcggcccg atcgccgtcg acgcgtgcac
      661 cgtcgagttc aatcgccccg cggtcgacga cccgacgctg tatccctcgg accatttcgg
      721 cctgagcgcg cggatccgcg taggaggctg agggcatgac gacgctccgg ttggcgcatc
      781 gaggcgactg gcgaatcgct cccgagaatt cgctcgcggc gatgcaggcg gcgctcacgg
      841 tgccgggctg cgacggcctc gagttcgata tccgaagctc gttcgacaac gtgcccgtgc
      901 tcctgcacga cgcgacgctg gcgcgcgtgc agcgtgtccc gctctcgtgc tcgacgctca
      961 ccgctgtcca gctcgcggag cacggcgtgc cgacgctcgg cgaggtgctg gcggcggtcg
     1021 gctgcgaccc gttcctcgac gtcgagctca aggagccggt caaaggcgcg atcgacgctc
     1081 tcgagctcga gcgcggcggc aacgacgaca aaggccaacc cgagctcaag aacgccgtgg
     1141 tgtcgtcgtt cgatgccccg gtgctcggct ggctgcgcag ccttcggccg acgtggccgc
     1201 gctggctgaa cgcgtatgac ctttcgccct cgacaatcgc gaccgcgacc gatctcggct
     1261 gcaccgcgat ctccgtggaa tggcacgggc tcgacgcgga cggcgtcgcc cgcgccacgg
     1321 attccggcct cggcgttgcc gcctggacgg ttcgcgagcc cgccgactac ctccggctcg
     1381 aggcgatggg tctgcttgcc gtctgcgctg aggccgccgc tctggaaggc tgagccgccc
     1441 atgctggatg tgcgccctca gcgagcgagg agctccgttc gaattcgcgc cccgatttcg
     1501 tctcggaccc gccggaacac ggcgagtcgc tcgtcatcgg tgccggtcgc tgccgacgga
     1561 tcgtcgaacg accaatgcag ctgggtcgta gcgcccggga agtacgggca ggcctcccgg
     1621 gcatcgtcgc agacggtgat ggcgtaatcg aacgcctgcc cggcaaatac gtcgctggtc
     1681 ttgctcgtct gtccggcgat atcgatcccg atctcgccca tcgccaggat cgcgagcggc
     1741 cgcacctcgg acgcgacgga gccggcgctc gccgcctcgt accgatcgga gccccacgac
     1801 cggagcatgc cctcggccat ctgcgagcgg gcgctgttgt gcacgcagag gaagaggacg
     1861 cggatcacgt tgttgattct acggacccca gtgtttccac cgggcgcgcg ggcgcggctg
     1921 ccgcaggccg cgcgatccgg gtcgtgatga tcgcgacggc ggcgatgacg acgatgccgc
     1981 cgagccactg gaccggctcc aggcgctcac cgacgagcca cccgcccgca aggccaaaca
     2041 ccgggatgag gtagaacgat gcggcggcga tcgaggctcg gaccgagcgc agcgccgtga
     2101 cgtagaacga gtaggcgaac gcgtagtaga ggagccccga ggccgcggcg ctggcgaggc
     2161 cgccgagcgt gagcgtccgc ggcgccatct gctggccggt cgcggcgagt gcgatgacga
     2221 ctcctacggc gagcgccagt gcgtggacct gctgcgccag caccacgctg aacgtcgagt
     2281 cggatccgag cagccagcgg cgggtcgcaa ccgtgtagat cgcacacacg acgacgccgg
     2341 ccacggtgag cgcgatgccc acggacgagc cggacgccgc cgggtcgaag acgaccagga
     2401 ccaacccgag gatcgccacc gcggacgacg cgatgatggg cagcccgacg cgctcaccga
     2461 ggaccagcgc cgcgaggccg aggatgaaga tcggctcgct cgcccagagc aggacggcca
     2521 agctcgcgct gatctgggtc agcccgacaa gactgagggc gtacgcaagg cccggattga
     2581 ggaggccgag gcgaccaagc agccgcccct cgcgccccgt cggcatccgc tcaccgcgaa
     2641 cccgcgtgag gacgagaagg aaaccgacgc tggttgcgag ctggatgggc agcaaggtca
     2701 gtggtggcat ctccgccacc gcctgcttgc tgatcacggt gccgatgccc cagcacgcgg
     2761 cagcgaggat cagccagagg acgttgcgcc gatcgccggg cccgatcatg gcgttcgacc
     2821 gccgatggcg cgcgaggctc aggcgatgac cgcgacgggc ttggtcgcgc gaatgatcgc
     2881 ggagtgcatg ccctcgacga tctggtgcgt gggcacgacc tcgacgtccg tcagcccgac
     2941 cgctcgcagg ccgtcgcgca tctcccggaa cgagagggct ccggcgatgc agccgacgta
     3001 ggtccctcgc tcggcacgct gctccgcggt cagcaggtcg tcggcgacga cgtcgcttac
     3061 gcccacgcgt ccgccgggac gaaggacgcg cgcaatctcg gcgaagaccg ccgacttgtc
     3121 cgatgccagg ttgatcacgc agttgctgat gacgacatcg acgctggcgt cgtcgagagg
     3181 gagcgcttcg atcgtccctt tgcggaactc gacgttcgtg gcgccggcgt cggcggcgtt
     3241 gcgtcgggcc aggctgagca tctcgtcggt catgtcgacg ccgatcgcgc gacccgtcgg
     3301 gcccacgcga cgcgccgaga gcagcacgtc gatgccacca ccggagccga gatcgagaac
     3361 ggtctcgccc tcgcgcagct ctgcgacggc agttggattg ccacacccga gcgatgcgag
     3421 gagcgctgcg tccgggagac tctggcgctc ggccgcgtca tacagctcga ggccgtaggt
     3481 acccgaaaca tcggagcagc agccgtcgcc gcagcaggcg tcggcctgtg gggcggcctg
     3541 ctcaactcgg gcgacagggc gagccgaacc gtcgacggcg acttccgacg tcgcgacggt
     3601 cggaccgcaa cactcgggcc cgcaacagct cgcctcggcg gtcgtgccgt gcgtcgcttc
     3661 gtgcttggtc aggcccgcgg cgacggcgat cgccgtctcc gcgtaccgat cgcgaacgat
     3721 ctgctggatc tcgttcagct cgaggctctg gtcgctcacg gtcgggtctc cttggtggac
     3781 agacggttca ggcggggcag gcgcaggcgt cggtggtcgg cgccggccgg cccgcgaacg
     3841 cgagtcggag gcggctcacg agactgggct tcgcgacgac gacggacggc gcgacttcgc
     3901 tgagccaatc gctcgcgatc cgtgcgtcgc ggagcgcgat ctcgtggaac tggttccgcg
     3961 ggtccgtgga gtgctctccg ccggacgtgt ggctgaggta gagctcgaac atttccggac
     4021 ctcctatctg tatcgacggc ggtcaatatg gcgcctatat agacgcttgt caatatcgaa
     4081 tttcatcgat accatcaccg ccatggcgaa gtccaccgac cccgacatcc agctcctggc
     4141 cgcgctcgcg gactcgaccc gcatggagat cctgcgcgag ctcgcggggg ccgccgaggt
     4201 ctgcgcgtgc gacttcaccg actgctgcga cgtctcgcaa ccgacgatct cgcaccacct
     4261 caaggtgctg cgcgatgccg gcgccgtcct gtccgaacgg cacggcaacc gcgtctacta
     4321 ccggatcgcg ccgaacctca tggagcgcct cggcgggatt gccggcggcc tggtgcccgg
     4381 cggcatggtt cctgcatcag cgctggcggt cggccggggc tcgatcgctt cgccgatccc
     4441 gttgggctcg agagcgacct cgaactagtc gcatcgcgcg cttccggagc gcgcctccgt
     4501 cagctcggca gcgcgcccgg cgggatgagc cagcggagcg acccggcccc agcggtcgga
     4561 tgtccatcgg ccaggtcgat ccgcgcgagc gcaccctttc ggaggtcgat cgcggccccg
     4621 gacagtgacg aggcgatgga gctgaagtcg gggtcgtgcc cgaccaggac gatgcgccga
     4681 gcatcgccgt ggcccgccag caacccgcca aggtccgcga ggtccatcgg cccggcgagg
     4741 cgcaggtcgt cggtggactt cgtgcccagc gcgcgaccga cgatccgggc ggtctgcgcg
     4801 gcgcggagct tcggcgaggt caggatgacg tccggccgga acccgagatc ggagaggagg
     4861 ctgccgaggc ggcgtgcctg gcgccggccc ttcgcggaga gcggtctatc ggcgtcgtcg
     4921 cccggccacg cagctaggtc gcccgcgtct gcatgccgga ggaggtacag ctgcagcggc
     4981 tggctgggaa ccgggatcgc aacaagcatc ggggctgcct cggcttcggg cgcggcagga
     5041 gccgtcgcgg tggtctcggt ggcgggcttg gtgggcttgg cgggcggacg tttcgggctc
     5101 gccggcgcct tgaccggcgc tgctgccttc gcgggtgact tggcgggggc cgccttggcc
     5161 gcggccgact tggccgcggc gggcttcgtc gatgccggct tcgctgacgg ccgtttcgct
     5221 gccgcgggct tcgcgatgga tgccgtcgtg ccagatgcgc gccgctttcg cttcggcgca
     5281 ggcgtgtcgc cctgatcggt catcgtcgat gccccactgc gagcggaagg aaggtatgga
     5341 tccggaccgc gagctcccgg agcgcctggt cgaacgcgac ctggcgctcg gcgtctgtgc
     5401 cggtcgcctc ggcgggatcg tcaacgcccc agtgcagggt ctcgctgcca cccgggaaca
     5461 ccggacaggt cgcacgagcc cggtcgcaga cggtgatgac gtagtcgaag cgctggccga
     5521 ggaactggcc gatcggcttg gacttcgcgt cggagatgtc gatgtccacc ttggccaacg
     5581 atcggatcgt gagcgggttc accggtcgcg ggctgacccc ggcgctcacg acgtggaacc
     5641 gctcgccgcc gtcccggcga aggatggcct cggccatctg gctcctggcg gagttgcccg
     5701 tgcacacgaa gatcacgcgg atcggctcgg ccggtcccgc cgcgggatcg ccggcacggg
     5761 gctcggaggc gctgatcacg ggttcaggtc ctcgatctgg cccgttgcga ggtacacgac
     5821 ctgctcggcg atgttcgtga cgcgatcgcc gatccgctcg acccagtggg cggcgaggat
     5881 gacgcgcgtc ccgcgttcga cggtgcgcgg gtcggaccgc atcaggtcga tgacctcggc
     5941 aaagatcgtg cgatagaggt ggtcgatatc gtcatcgcgc gccgcgaccg tccgcgccag
     6001 gtcgtcatcg atgtcgatca gcgcggtcac gatgcctcgc acctggtcgg cagcgaggcg
     6061 gcccatctcc acgagatcac gcgtctcgcg cagcacggat tcctcggtga ggcgccgcgt
     6121 ctgcttggcg atgctcgagg cgtgatcgcc gatccgctcc agctcgaatg cgacgtgatc
     6181 caggcccatg agaaagcgca gatcgcgcgc gacgggggat tgcgtcgcga tcgtcgtcac
     6241 gaccgcgtcg gcgatctcgc gctggacctc gttgatccgc tcgtcggcag cgatcacccg
     6301 atcggcggcc tcgacgtcgc gctcggcaac ggagcgcacc gcctcgcgga tctgggcttc
     6361 gacgagcgag cccatccgca ggatcccgtc cttgaccacg cgctcgtcgc gatcgagccc
     6421 ggcgcgcgag cgcgtcgggt cgatgcgaat ggctccggcc gctgtctcca tgttctcccc
     6481 tctacccgaa gcgccctgag atgtagtcct cggtgagctg ctcgcgaggg ttcgtgaaga
     6541 tcttgatggt cttgtccatc tccacgaggt agcccgctcg atcctcgccc atcgtgagga
     6601 atgccgtgag gtcggacgcc cgggccgcct gctgcatgtt gtgcgtgacg atgacaatcg
     6661 tgtaccggcc gctgagctcg cgcatgagct cctcgatctg gagagtcgct cgcgggtcga
     6721 gggccgagca cggctcgtcc atcaggatga tctcaggctc cgtggcgatc gcacgggcga
     6781 tgcacaaccg ctgctgctgc ccaccggaca gcgcgagcgc cgactggccc agcttgtcct
     6841 tgacctcgtc ccacagggcc gcgcgtcgga ggctgccctc gacgaggtcc ttcgtgttgc
     6901 ccttgaaccc gttgatcctg gccccaaacg ccacgttctc gtagatcgac ttcgggaacg
     6961 ggttcggctt ctggaagacc atgccgacgc ggcgccggac ggcaaccgga tcgaccgcct
     7021 tgtcgtagag gtcctggtcg tcgagcagga tgctgccctc gaggtggaag ctcgggacga
     7081 ggtcgttcat ccggttcatc gtccggagca cggtcgactt gccgcagccc gacggcccga
     7141 tgagggcggt gacaccgtac cgggcgacct tgagcgacac gtcgcggacg gcacggaacg
     7201 agccgtagta gcacgagacg ttgcggagct cgagggtcac gtcgggcgcc gcagcctcgg
     7261 ctgcgtcggc ggtctgcacg ctgggagtat cgaacgggac gatgcggatt gccgcaggct
     7321 cctgttcgtt gtcgggcgcc cgagggttca ccgggggcgc ttcggtcgtc tgcatggaca
     7381 tggacgattc ctctaccact ggagatgccg tgacaggcgt gcgcgaacga ggatcgcgag
     7441 cccgttgagg acgagcatga ggagcaggag gaccaggatc gccgccgcgg caatgccctg
     7501 gaaggcctcc tggggccgac tggcccagtc gtagacctgg atcggcagga cggtgaagcc
     7561 ggtcttgagc gggctgggca ggaacgtgac gaagctcgcc gcgccgatca cgatgagcgg
     7621 cgccgcctca ccgatcgctc gggccatggc caggatgatg ccggtcagga tcccgggcgc
     7681 agccgagggc aggaccgatc gacggaccat ctgccagcgc gtggcgccga gggcatacgc
     7741 cccttcgcgc tgtgaatcgg gcacggcccg cagcgcctcc atcgtggcga tgatgacgat
     7801 cggcatgatc aacagggcca gtgtgagggc cgccgcaagt ggcgagcggc cgaggccgag
     7861 gacgcgcacg aagagggcca gcccgaagat gccgtagatg atcgacggca cgccg
//
LOCUS       DATBSW010000201         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_18916, whole genome shotgun sequence.
ACCESSION   DATBSW010000201 DATBSW010000000
VERSION     DATBSW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18916"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VIF63_05645"
     CDS             complement(<1..185)
                     /locus_tag="VIF63_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238900.1"
                     /translation="MSSRRIMTSDEIRRAVIRISHEIVEKQAGTAGLVLVGIQRRGVP
                     LAHRLAEAIAEHEGATLP"
     gene            complement(265..828)
                     /locus_tag="VIF63_05650"
     CDS             complement(265..828)
                     /locus_tag="VIF63_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238901.1"
                     /translation="MRPSTTVARAALSAAFALGALPGLVGSREPNQDLPLAAALFEPV
                     DLATARDRQGLGAIVPDSAGRSAAALDALEAFGEPGTNDSGPPEERAQPKVPSGKADW
                     RWKAPRFSLTGTASFYDFGTTAMRDVPRGTRIVICGAGGCLETIVSDYGPSKAGGRLI
                     DMYRPDFFRICGCGWWSGTTTVTVRVY"
     gene            complement(886..>2289)
                     /locus_tag="VIF63_05655"
     CDS             complement(886..>2289)
                     /locus_tag="VIF63_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238902.1"
                     /translation="RAANDGQWELILADVKAVCPRSRPNCGQLVEDDPGRTINLGSAP
                     TGGFTISENEQQLVIERNAEGAAPGRILVVPIPPASPAVTPAPVDTPKPVDTPKPVDT
                     PKPVDTPSPADTPTPPPPTGEPVSPAVPTPVPTSTTPDGTIEIASGVTMVGEAAYSSD
                     SKWLAFSARPSDGSTGPDLYLWSVGQPTAIAVTSNHRTYFSTWLGDRILASSVDDVVA
                     PTGSALPAESALPTEIPTIEPTAAPSNSPAPAVEAHPSSFLLDPATLTRTEITQPDVW
                     MPVVDPTGRFVAYWSGTLAATADGIDWQLATGRLVLDGWSDGAPEPTTGPTAEPTDAP
                     AVGSEAPVASPVPALGPVGTPVEVVTGATAAFKAKFDPSGSRLAIWAGEQVDATVGRL
                     HLIVLDPATGAIQPDIAPLPGAPALKRFSIDEGRLAWVSPSGQDGQESAVQVLGWSAD
                     QFGEIRTVPAKDLYIVR"
BASE COUNT          308 a          795 c          857 g          329 t
ORIGIN      
        1 ggcagggtgg cgccctcgtg ctcggcgatc gcctcggcga gccgatgcgc caggggcacg
       61 ccgcggcgct ggatcccgac gaggacgagc ccggccgtgc cggcctgctt ctcgacgatc
      121 tcgtgggaga tgcggatcac cgcccggcga atctcgtcgg aggtcatgat ccggcggctg
      181 ctcatcgacg acctcccggc ctcacggggc caacatccgg ctcacgcggc cgggctcgaa
      241 ggtggtaccg agtgtaccgc ccgatcagta gacgcggacc gtcaccgtcg tcgtgcccga
      301 ccaccagccg cagccgcaga tccggaagaa gtccggccgg tacatgtcga tgagccttcc
      361 gcccgccttc gacggcccgt agtccgagac gatggtctcg aggcagccgc ccgcgccgca
      421 gatcacgatc ctcgtgcccc ggggcacgtc gcgcatcgcc gtggtcccga agtcgtagaa
      481 cgaggcggtc ccggtcaacg agaaccgcgg cgccttccag cgccaatcgg ccttgcccga
      541 cggcaccttc ggctgggcgc gctcctccgg tgggccggag tcgttggtcc ccggctcgcc
      601 gaaggcctcg agggcgtcga gggcggcggc ggaccgacca gctgaatcgg ggacgatggc
      661 cccgaggccc tgccgatcac gcgcggtcgc gaggtctacc ggttcgaaca gcgctgcagc
      721 gagaggcagg tcctggttcg gctctcgcga gccgacgagc cccggcagtg cgccgagcgc
      781 aaacgcggca ctgagcgccg cccttgcgac cgtcgtgctg ggtcgcatcc ggcggccact
      841 gtacggcatg tcgcgcccga ggcgcgcgtc aaatggtccc cggaatcagc ggacgatgta
      901 caggtccttg gccgggaccg tacgaatctc gccgaactgg tcggcggacc aaccgagcac
      961 ctgcacggcg ctctcctggc cgtcctggcc gctcgggctc acccacgcga gccggccctc
     1021 gtcgatcgag aagcgcttca gcgcgggggc gcctgggagt ggcgcgatgt caggctggat
     1081 cgcgccggtc gccgggtcga ggacgatcag gtgcagccgt ccgaccgtgg catcgacctg
     1141 ctcgccggcc cagatcgcga ggcgcgagcc gctcgggtcg aacttggcct tgaacgctgc
     1201 cgtggcaccg gtgacgacct cgactggcgt cccgacagga ccgagcgccg gcacggggct
     1261 ggcgactggt gcttcggatc cgaccgcggg cgcatcggtc ggctcggcgg tcggcccggt
     1321 tgtcggctcg ggcgcgccgt ccgaccagcc gtcgagcacg agccggccgg tcgcgagctg
     1381 ccagtcgatg ccgtccgccg tggcggccag cgtgccggac cagtaggcga cgaagcgacc
     1441 tgtcgggtcg accaccggca tccacacgtc gggctgggtg atctcggtgc gcgtcagcgt
     1501 ggcgggatcg aggaggaacg acgacgggtg tgcctccacc gcgggcgcag ggctgttgct
     1561 cggcgcggca gtcggctcga tcgtcgggat ctcggtcggc agcgcgctct cggccggcag
     1621 cgcgctcccg gtcggcgcga cgacgtcgtc cacggagctg gcgaggatgc gatccccgag
     1681 ccaggtggag aagtaggtcc ggtgattgct ggtgaccgcg atcgcggtcg gctggccgac
     1741 cgaccacagg tacaggtccg ggccggtgga gccgtcgctc ggacgcgcgg aaaaggccag
     1801 ccacttggag tcgctcgagt acgccgcctc gccgaccatg gtcacgccgg atgcgatctc
     1861 gatcgtgccg tcgggcgtcg tcgacgttgg cacgggggtc ggcaccgccg gcgagactgg
     1921 ctcgcccgtg ggcggcggcg gagtcggcgt gtcggcagga ctcggggtat cgaccggctt
     1981 cggggtatcg accggcttcg gggtatcgac cggcttcggg gtatcgaccg gggcgggtgt
     2041 caccgcgggg ctcgcgggcg ggatcgggac cacgaggatc cgacccggcg ccgcgccctc
     2101 ggcattgcgc tcgatgacga gctgctgctc gttctccgag atcgtgaagc cgcccgtcgg
     2161 ggcactgccg aggttgatgg tccggcccgg gtcatcctcg accagctggc cgcagttggg
     2221 ccggctgcgc gggcagacgg ccttgacgtc ggcgaggatc agctcccact gcccgtcgtt
     2281 cgcggcccg
//
LOCUS       DATBSW010000202         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_54290, whole genome shotgun sequence.
ACCESSION   DATBSW010000202 DATBSW010000000
VERSION     DATBSW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54290"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VIF63_05660"
     CDS             <1..612
                     /locus_tag="VIF63_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY8238903.1"
                     /translation="AAVVPHRTWIDPFVPWILLPERPRFTFFGDARTMARSPLRRFLV
                     RRLGGVIPIPAARDPQTVAVHLAAAAAALDAGSVFMLMPETGPASAAGAIRRLGGGMA
                     YVALRSGAPIVPLILGGNDELYLGRRVILRVLPALDPRELAGLAPGGELPESGSSAER
                     RAARTLTTAFAASVDGAVADVHARSIAAPGQRKRGRFLTTLFR"
     gene            680..1363
                     /locus_tag="VIF63_05665"
     CDS             680..1363
                     /locus_tag="VIF63_05665"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEY8238904.1"
                     /translation="MEFIGDLERAFATSLYPLRVPIFFGLLIASVAFVWYARRRGWFT
                     VARRHPGRTSAMLIVLLVVGLPVGWYLASPLWIRTALNEPLPAVTPTNAPPTTVVIAP
                     SAPPVTGAPPVTGAPPVLSATRTGEFEGEDEFHFGSGTASLIETEPGTFVVRFENFSV
                     RNGPDLFVYLSPEPDGYGGDVLELGTLKATDGNFNYTVPAGMRPGEYKSIVIWCKQFA
                     VQFAVARLT"
     gene            complement(1356..1988)
                     /locus_tag="VIF63_05670"
     CDS             complement(1356..1988)
                     /locus_tag="VIF63_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238905.1"
                     /translation="MRGRDLALGRRARNDNIESVAGECAGHGGGERPRREWHAIEAVT
                     GRRSQQQLQHPTRRYEPGNACGNPRIAADAAAQERSPWELHGDARRGAAELGRRRFCR
                     GEDEAGIRQTALAGRPGRTARRFGHGGGDRVQAEREGSRFPGGGGEDGSAVARPDVHR
                     HPLVAGNEIGNLADVHLEEAAANDELDHGRQDTLAAQQAPRVVGSGTEIR"
     gene            2068..2349
                     /locus_tag="VIF63_05675"
     CDS             2068..2349
                     /locus_tag="VIF63_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238906.1"
                     /translation="MTSFLANETVRRIAATLGVLGSLALAYLYILYPLLTVPGLARYG
                     FYGAWIVLVVLSLMWWRRHPWRALAVPVVGLVAVLAALWFGGEYLGWAP"
     gene            2346..3392
                     /locus_tag="VIF63_05680"
     CDS             2346..3392
                     /locus_tag="VIF63_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY8238907.1"
                     /translation="MRVPLILDVDTGIDDALALLYACASPEVELLAVTCVAGNVDARQ
                     VALNTRAVLELAGRADVPVLIGEGQPLHKPLQTTPETHGPKGIGHAALPAPVRELEPD
                     HAADRIVALAHARPGEITLVTLGPLTNVAVALGEPALPSLLGGFVVMGGAFRAPGNTT
                     PTTEWNMHVDPDAAKAVFAAWAGAPAGLARPLAMGLDVTEQARLLPAHLGRLALRAGA
                     SKRDAGAFASEPIRVIGSVAANPILRFIGDAVRFYFEFHDRYDGFYGAFIHDPFVVAA
                     ALDRALVTTQPVFVDVEAGPGLAHGMTVADWRGLTGKPANLDVAVAADAEAFLDRFIE
                     RVGALAGARAGVAP"
     gene            complement(3420..3953)
                     /locus_tag="VIF63_05685"
     CDS             complement(3420..3953)
                     /locus_tag="VIF63_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY8238908.1"
                     /translation="MVDDATRAADGAPTRGTAEYYPGAAGLAHPGPASVHLSPEIRDG
                     IVEHARREAPNEACGIVVGDRSASEGGSAVRWVPLRNPLASPFRYSIDPDDLLRLTIE
                     TDDADDVFWAIVHSHVESPARPSPTDLREAHYPDALYVLVSLSASEADQATGTPSLRA
                     WRIVDGGVHEVALTIDA"
     gene            complement(3947..>4389)
                     /locus_tag="VIF63_05690"
     CDS             complement(3947..>4389)
                     /locus_tag="VIF63_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8238909.1"
                     /translation="ELDVFVAGLGTGGTLTGVGRFLRRAKPGVRIIAAEPLPGEAVQG
                     LRSLDDGFVPEIFDPTLLDDRYVVSNAEAISIARLLLEREGIFAGPSSGAILAVAGRV
                     AQTMDSGTIVALLPDGGWKYLSAGTYTKPIDEMENELEGGASWW"
BASE COUNT          566 a         1604 c         1526 g          693 t
ORIGIN      
        1 gcggcggtcg tcccgcaccg cacctggatc gacccgttcg tgccctggat cctgctgccg
       61 gagcggcccc ggttcacgtt cttcggcgac gcccggacga tggcccgatc gccgctgcgc
      121 cggttcctcg tccgccgcct cggcggcgtg atcccgattc ccgcggcgcg cgatccacag
      181 accgtggcgg tgcacctggc ggcggccgcg gcggcgctcg atgcgggctc ggtcttcatg
      241 ctcatgcccg agaccggccc ggcgtcggcc gccggcgcga tccgccgcct cggcggcggc
      301 atggcatacg tcgcgctccg cagcggcgcc ccgatcgtgc cgctgatcct cggcgggaac
      361 gacgagctgt acctcggccg gcgggtcatc ctgcgtgtct tgccggcgct cgatccgcgc
      421 gagctggccg ggctggcgcc cggcggcgag ttgccggaat cgggctcctc ggcagagagg
      481 cgagcggcgc gcacgctgac gaccgcgttt gccgcgagcg ttgacggtgc cgtcgccgat
      541 gtccatgccc gctcgatcgc ggcgcctgga cagcgcaaac gcggacggtt cctcaccacg
      601 ctcttccgct gaccgccgac ggccggcaac cgcggcggcg tcatcgaccc gtcattcgcc
      661 gtaagggatg atccgcgtga tggaattcat cggcgacctc gagcgcgctt tcgcgaccag
      721 cctgtatcca ctgcgggtcc cgatcttctt tggcctgctg atcgcgtccg tcgcgttcgt
      781 ttggtacgcg cgtcggcggg gctggtttac ggtcgctcgg cgccatcccg ggcgcacgag
      841 cgcgatgctg atcgtgctcc tcgtcgtcgg cctgccggtg ggctggtacc ttgcctcgcc
      901 cctttggatc cgaaccgcgc tcaacgaacc attgcccgcc gtgaccccaa cgaacgcgcc
      961 gccgaccacg gtggtgatcg ccccgagcgc gccgcccgtg accggggcgc cgccggtgac
     1021 cggggcgccg ccggtactga gcgcgacgcg gaccggagag ttcgagggcg aggacgagtt
     1081 ccacttcggc agcggcacgg ccagcctcat cgagaccgag cccgggacgt tcgtggtgcg
     1141 cttcgagaac ttctcggtgc gcaacggccc ggacctgttc gtgtacctgt cgccggagcc
     1201 ggacgggtac ggaggcgatg tcctcgaact gggcaccctg aaggccaccg acggcaattt
     1261 caactacacc gtcccggccg gcatgaggcc cggcgagtac aagagcatcg tgatctggtg
     1321 caagcagttc gcggtccagt tcgccgtcgc ccgcctcacc tgatctcggt tcccgagcca
     1381 acgacccgcg gggcctgttg cgcggccagg gtatcctgcc gcccatgatc aagttcgtcg
     1441 ttggccgccg cctcctcgag gtggacgtcg gctaggttgc cgatctcgtt gcccgccacg
     1501 agcgggtgac gatggacgtc gggacgggcg acggccgagc cgtcctcgcc gccgccgccc
     1561 gggaacctcg aaccctcgcg ctcggcctgg acgcggtcgc cgccgccatg gccgaatcgt
     1621 cgcgccgtgc ggccgggccg gcccgcaagg gcggtctgcc gaatgcccgc ttcatcctcg
     1681 ccgcggcaga atcgcctccg cccgagctct gccgcgccgc ggcgcgcgtc accgtgcagt
     1741 tcccatgggg atcgctcctg cgcggctgcg tcggcggcga tccgagggtt gccgcaggca
     1801 ttgccgggct cgtagcgccg ggtgggatgc tggagctgct gctggctccg tctgcccgtg
     1861 acggcctcga tggcgtgcca ctcgcgcctg gggcgctcac cgccgccgtg gccggcgcat
     1921 tctccagcca cggactcgat gttgtcattg cgcgctcggc gaccgagggc gagatcgcgg
     1981 cctcgcattc gacctggtcc cgccgtctcc gatcgcaaca acctgttgat cgaggggtca
     2041 tgctggttcg gctcgtgagg tgcagcgatg acatcgttcc tggcaaatga gaccgtccgc
     2101 aggatcgccg cgaccctcgg tgtgctcggt agcctggcgc tcgcctacct ctacatcctc
     2161 tacccgttgc tgaccgtgcc gggactggcc cgctacgggt tctacggggc ctggatcgtc
     2221 ctcgtggtcc tctcgctcat gtggtggcga cgtcacccgt ggcgcgcgtt ggcggtcccg
     2281 gtcgtcgggc tcgttgccgt gctcgccgcc ctgtggttcg ggggcgaata cctgggctgg
     2341 gcgccttgag ggtcccgctc atcctcgatg tcgacaccgg gatcgacgac gccctcgccc
     2401 tcctctatgc ctgcgcgtcg cccgaggtcg agctgctcgc cgtgacctgc gtcgcgggca
     2461 acgttgatgc ccggcaggtc gcactcaaca cccgcgccgt gctggagctg gcgggtcgcg
     2521 ccgacgtgcc ggtgctgatc ggcgaggggc aaccgctcca caagccgctg caaaccacgc
     2581 ccgagacgca cggcccgaag ggcatcggcc acgcggcgct gcccgcaccg gtgcgcgagc
     2641 tcgagcccga ccacgcagcg gaccggatcg tggcgctcgc ccacgcccgg ccaggcgaga
     2701 tcacgctcgt gacgctcgga ccgctcacga acgtcgccgt tgcccttggc gaacccgcgc
     2761 tcccgagcct gctcggcggc ttcgtggtca tgggcggcgc cttccgcgcg ccgggcaaca
     2821 cgaccccgac gaccgaatgg aacatgcacg tcgacccgga cgccgcgaag gccgtgttcg
     2881 cggcctgggc tggggcgccc gcagggctcg cgcgtccgtt ggcgatgggt ctcgacgtga
     2941 ccgagcaggc gcggctgctc ccggcgcatc tcggccggct ggcgctgcgc gcgggcgcgt
     3001 cgaaacgaga tgccggcgcg ttcgcctccg agccgatccg cgtcatcgga tcagttgccg
     3061 cgaacccgat ccttcgcttc atcggtgacg ccgtgcgctt ctacttcgag ttccacgatc
     3121 gctacgacgg cttctacggc gcattcatcc acgatccttt cgtggttgcg gcggcgctcg
     3181 atcgagcgtt ggtgacgacc cagccggtct tcgtcgatgt cgaggcgggg cccgggctcg
     3241 cccacggcat gaccgtcgcc gactggcgcg ggctgacggg caagcccgcg aacctcgacg
     3301 tcgccgtcgc ggccgacgcc gaggcgttcc tcgaccgctt catcgagcgc gtgggagccc
     3361 tcgcaggtgc gcgcgcgggc gtggcaccct agcccgaacc ggcgaaggga ggtctcggac
     3421 taggcgtcga tcgtcagcgc gacctcgtgg acgccgccgt cgacgatccg ccaggcgcga
     3481 aggctcggcg tgccggtcgc ctgatcagcc tccgaggcgg agagcgaaac cagcacgtac
     3541 agcgcatctg ggtagtgcgc ctcgcgcagg tcggtcggcg atggtcgcgc gggtgactcc
     3601 acgtgcgagt ggacgatggc ccagaacacg tcgtcggcgt cgtcggtctc gatggtcagt
     3661 cggagcaggt cgtcggggtc gatcgagtag cggaacggcg acgcgagcgg gttccgcagc
     3721 ggcacccaac gcaccgccga gccgccctcg gaggcagagc gatcgccgac aacgatcccg
     3781 caggcctcgt tcggcgcctc gcgccgggcg tgctcgacga tcccgtcgcg gatctcgggg
     3841 gagaggtgca cggacgcggg cccgggatgg gcaagccccg cggcgcccgg gtagtactca
     3901 gccgtgcccc gagtgggcgc gccgtccgcg gcgcgagtcg cgtcgtctac caccagctgg
     3961 caccgccctc gagctcgttc tccatctcgt cgatcggttt ggtgtacgtg cccgccgaaa
     4021 ggtacttcca gccgccgtcc ggcagcagcg ccacgatcgt cccggaatcc atcgtctggg
     4081 caacgcggcc tgcaaccgca aggatcgcgc ccgacgacgg ccccgcaaag atcccctcgc
     4141 gctccagcag cagccgcgcg atggagatcg cttcggcgtt cgagacgacg tagcggtcgt
     4201 cgagcagggt cgggtcgaag atctcgggca cgaacccgtc atcgagcgag cgcaacccct
     4261 ggactgcctc gcccggcagc ggctcggccg cgatgattcg cacgcccggc ttggcccgtc
     4321 gcaggaaccg gccgacgccg gtcagcgtcc cgccggtacc caacccggcc acgaacacgt
     4381 cgagctctg
//
LOCUS       DATBSW010000203         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_900200, whole genome shotgun sequence.
ACCESSION   DATBSW010000203 DATBSW010000000
VERSION     DATBSW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900200"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VIF63_05695"
     CDS             <1..1015
                     /locus_tag="VIF63_05695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY8238910.1"
                     /translation="ARTLASQRQLLVLNERFLATVDPQQVLSLLADSLHSLVAYDNLT
                     IYRVDRAAGLLRPVLARDRFASLIMESTITLDRGITGWVVTHGEAQCVNDAMQDSRIS
                     LIPGTPSEDESLIVVPLTADGMVLGTLNVGRMGGPEAYFSATEFEIARLFASQASIAL
                     VNAESHRAVATRAETDALTGLRNRRAFDDDMVGLMADPAAPPIVLVMLDLDGLKQFND
                     RFGHPAGDKLLRAVAEAMSASVRGDDRAYRIGGDEFAVLMRKTSLRVGMQVASRIRRA
                     IADLDSGGGSAVAASLGVAACPEDVADPSALVSAADAALYRAKRAGGNRVDGVERLLS
                     GRV"
     gene            complement(999..1559)
                     /locus_tag="VIF63_05700"
     CDS             complement(999..1559)
                     /locus_tag="VIF63_05700"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY8238911.1"
                     /translation="MSAAADDADNPSMAPHRQQVTTIVVMGVAGAGKSSVMTALAAKL
                     GWPTLEGDDLHPAPNVAKMAAGVPLTDDDRAPWLDAIAAAIGEREVGGSSSVVTCSAL
                     RRRYRDVLRRGHPSVRFVHLVAARDELASRMRNRVGHYMPPELLTSQLETLEPLEADE
                     PGSTIETSGTPDEMAERIVAALRLDR"
     gene            1653..>2107
                     /locus_tag="VIF63_05705"
     CDS             1653..>2107
                     /locus_tag="VIF63_05705"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY8238912.1"
                     /translation="MAATRSKVIISCAITGSIHTPTMSPYLPITPQQIAQASIEAAQA
                     GAAILHLHARDPRDGRPTGNPDVFMEFLPQITAACDAVINITTGGGQHMTLEERIAGA
                     VAAEPELASFNMGSMNFALYPMLARYDKFEHEWERAHLENSRDFVFRNT"
BASE COUNT          331 a          749 c          680 g          347 t
ORIGIN      
        1 cgcccggacg ctggcgagcc aacgccagct cctcgtcctc aacgagcggt tcctggcgac
       61 cgtcgacccg cagcaggtcc tcagcctcct cgccgactcg ctgcattccc tggtcgcgta
      121 cgacaacctg accatctacc gagtcgatcg tgcggccggc ctgctccgcc ccgtcctggc
      181 acgggatcgc ttcgcgtcgc tgatcatgga gagcaccatc acgctcgatc gcggcatcac
      241 gggctgggtt gtgacccacg gcgaggcaca atgcgtcaac gacgcgatgc aagattcccg
      301 gatttcgctc attccgggga caccgtcgga ggacgagtcg ctgatcgtcg tgccgctgac
      361 cgcggacggc atggtcctgg gaaccctcaa cgtcggccga atgggcgggc ccgaagccta
      421 cttctcggcc acggagttcg agatcgcccg gctctttgcc agccaggctt cgatcgccct
      481 tgtcaacgcc gaatcgcacc gggccgtcgc gacccgggcg gagactgatg ccctgaccgg
      541 cctccgcaat cggcgcgcct tcgacgatga catggtcggt ctcatggccg acccggccgc
      601 cccgccgatc gtgctcgtga tgctcgatct tgacggcctc aagcaattca acgatcgctt
      661 cggtcacccg gcgggcgaca agctcctccg tgccgtcgcg gaagccatga gcgcatcggt
      721 ccgcggcgat gaccgcgcct accgcatcgg cggcgacgag ttcgctgtcc tcatgcggaa
      781 gaccagcctt cgcgttggga tgcaggtcgc gagtcgaatc cgacgcgcga tcgccgacct
      841 cgattcgggc ggcggctcgg cggtggctgc cagcctcggc gtcgccgcct gcccggagga
      901 cgtcgcggac ccatccgcgc tcgtcagcgc tgcggacgcc gccctgtacc gagcgaagcg
      961 ggccgggggc aatcgcgtgg acggcgtgga gcgcctcctc agcggtcgag tctgagcgcg
     1021 gcaacgatcc gctccgccat ctcgtcaggg gtgccggaag tttcgatcgt cgagccgggt
     1081 tcgtcggcct cgagtggctc cagcgtttcg agctgcgacg tcaggagctc cggcggcatg
     1141 tagtgcccga cccggttcct catccgcgat gccaactcgt cgcgtgcggc cacgaggtgg
     1201 acgaaccgca ccgacggatg gccgcgtcgg agcacgtcgc gatagcgccg ccgcagggcc
     1261 gaacacgtga cgaccgagct ggagcctccg acttctcgtt caccgatggc tgccgcgatg
     1321 gcgtccagcc atggcgcacg atcgtcgtcg gtgagcggca cacccgcggc catcttcgcc
     1381 acgttcggcg cgggatgcag gtcgtcgccc tcgagcgtgg gccagccgag cttcgccgcg
     1441 agggccgtca tgacgctcga cttgccggcg ccggccacgc ccatcaccac gatcgtcgtc
     1501 acctgctgtc gatgcggcgc cattgaaggg ttgtccgcgt cgtctgctgc tgcactcacg
     1561 atgcgcgaag tatgggtcgc attcggaggc attcggtgcg cgcggacgtt cgcattggcc
     1621 caagatcacc gagtcacgtt tcggaggctg acatggcggc gacgcgatcg aaggtcatca
     1681 tcagctgcgc gatcaccggc tcgatccaca cgcccacgat gtcgccatat ctcccgatca
     1741 cgccacagca gatcgcccag gcgtcgatcg aggccgcgca ggccggcgcc gcgatcctgc
     1801 acctccacgc tcgcgacccg cgcgatggtc gaccgaccgg gaaccccgat gtcttcatgg
     1861 agttcctgcc ccagatcacg gcagcctgcg acgcggtgat caacatcacg accggcggcg
     1921 gccagcacat gaccctcgag gaacgcatcg ccggcgcggt ggcggcagag cccgagcttg
     1981 cctcgttcaa catgggctcg atgaacttcg cgctctatcc gatgctcgcc cggtacgaca
     2041 agttcgagca cgagtgggag cgggcgcatc tcgagaacag tcgcgacttc gtcttccgga
     2101 acacgtt
//
LOCUS       DATBSW010000204         7681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_830200, whole genome shotgun sequence.
ACCESSION   DATBSW010000204 DATBSW010000000
VERSION     DATBSW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830200"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4408
                     /locus_tag="VIF63_05710"
     CDS             <1..4408
                     /locus_tag="VIF63_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8238913.1"
                     /translation="TDAQIKVLIDAGEFESVGAQTGDYLGVPLKNESRTVGVLAVQSY
                     VEGIGYDQEDERLLAYVARHIAAALERTRATAEIRQRNAELALINEVGDALAKQLDFH
                     AIVELIGERLARMFNSLDVFIAMYEKVAQQITFPYELDQGRRVHSDPIPFGPGLTSEV
                     LRTGRPLVFGTAAEQEIHSPVTTAFAEGGMASEAGSWLGVPIMSGDEAIGVIAFSEYR
                     ENAFTDADVRLVTTIVTSMGVALENARLFEQTNTLLAETKERAAELAIINSVQEGLAA
                     KLETQAMYDLVGDKIREIFDAQVVDIGILDRENGIFHFPYSIERGVRFPDEPMGIVGY
                     RKVVFETKAPLLLNRVSVRFAAEVGQGAAIQGEDPLSLLFAPLVTGGDVTGVISLQNL
                     DHEDAFSDSDVRLLTTLAGTLSGALENVRLFDETKRLLAESTERAAELEIINSIQQGL
                     AGRLEMQAMYDLVGEKIAEIFDVHGVDIESYDRSTEMIHFAYTVERGERLAADPIPLF
                     GFRRQVVETRAPVVVNRDLEARAAEVGQPATIAGELAKSVIFVPMIIAREVTGIILIE
                     NLEREDAFSDGDVRLLTTLAASLSVALENARLVAETRQRASELLIVNEVGQAAASQLD
                     LDRLIQLTGEQLRTTFRADIVYIALLNDDTGLIDFPYRTERGKVTPRPPMRVGEGLTS
                     QILSSREPLLLNRADQFSVMEQKVGTAVKSYLGVPILVGDRAIGAVSVQSIDEAGRFG
                     ETDARVLSTIASNVGTAIRNAQLYREAQRRQQETAALNEVGREISATLDLEVVLGRIA
                     ERAQVLSGAATSAVFLVQPDGRTLKAITVVGEAVDEIKADEFTLGEGIIGTAAAERRP
                     EIVNDVLRDPRSVTIAGTFEDDEEEERLMSAPLLGRQGLSGMMAVWRAGAAPAFTQAD
                     LDFLVGLSQQAAIAIDNARLFADADVARNAADEANQAKSAFLAAMSHEIRTPMNAIIG
                     MSGLLLETTLDEEQRDFADTIRTSGDALLTIINDILDFSKIEAGKVDLVHEPFALAAS
                     IEGALDLIAPTAGKKGIELAYEVAGDLPPAVAGDQGRLRQIVLNLLSNAVKFTDSGEV
                     VVSVRSSPFGARWEVAVEVRDTGIGIPADRIGRLFQSFTQADSTISRRFGGTGLGLAI
                     SRRLAEAMDGSLVAESSGVAGEGTTFKLLVRLDAAEASAVPERRDRDLVEIAGRTALI
                     VDDNATNRRILTAQLARWSMTTRETASAAEALAWIRAGEQFDVVLLDLQMPEMDGLAL
                     ADAIRAIEQPTQPKLILVSSIGIRERGNAALDAVLTKPIKPSALHDALVNVLSVGGGA
                     GEDRLQRAPERPAVDPELAQRHPLRILLAEDNAVNRKVAQRLLANMGYAADMAENGLL
                     ALEAIEAKEFDVVLMDVQMPEMDGLEATRQIRARWPERPIHIVAMTANAMAGDRDLCL
                     AAGMNDYVSKPIRVPELAAALARTPSLADVTR"
     gene            4405..4770
                     /locus_tag="VIF63_05715"
     CDS             4405..4770
                     /locus_tag="VIF63_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8238914.1"
                     /translation="MTADDAPILDDGVIAELRASTGDDDDFVRELVEAYIEEGRGYIA
                     AMNTAAAAADAEAMVRPAHTLKSSSATMGAMRLAAICRGIEEAARDGENEGLAADVDA
                     ATAAWSATLEAMAAAGLTA"
     gene            4767..5939
                     /locus_tag="VIF63_05720"
     CDS             4767..5939
                     /locus_tag="VIF63_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8238915.1"
                     /translation="MNASRAVALVIDDSLVNRKLLTRLLATLDIEAREAANGRTGLEL
                     LRSSEADVAVVLLDIVMPEMDGYETLAAIKADESLRHLPVIMISGIEELDSVVRCIEL
                     GATDYLPKPFDAVILGARIRASLAGKQLHDLQIESERRQAELLRTIKAQKEELSRFFS
                     PQVAALVSSPEGVQMLAGHRRVATSLFADLRGFTPFSEATDPEVVLDVLREYHVAMGA
                     LIVAAGGTVEHFAGDGMHVFFNDPIVQDDHVERAVRVAVDMRDRFAELDAGWTARGYT
                     LGFGVGIATGFATLGRIGWEGRYDYGMVGTAVIVAARLSSVALANQVLLSEESYAEVR
                     DLVDVEPVGELHLKGFSRPIVPLNVLRFRSPDSPASSASLAALASPALPALPASST"
     gene            complement(5836..>7681)
                     /locus_tag="VIF63_05725"
     CDS             complement(5836..>7681)
                     /locus_tag="VIF63_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8238916.1"
                     /translation="PRVVMIAKSTYVWLDQLSRTYRRDIRTLDAIPDDELDALARWGV
                     TGLWLIGLWERSIASERIKRMRGNADAVASAYSLDDYRIADDLGGEGAYADLRDRAWT
                     RGIRLASDMVPNHMGIDSRWVIEHPERFLSVNEPPFPAYTFGGPNLSPDPRVDLQIED
                     HYWDGSDAAVVFKRRDTASGEQRFIYHGNDGTSYPWNDTAQLDYLRADVREAVIQTIL
                     DVARRAPILRFDAAMVLARRHIRRLWYPEPGAGGAIPSRAEHALSNAEFNRRMPAEFW
                     REVVDRVAAEAPGTLLLAEAFWLLEGYFVRTLGMHRVYNSAFMHMLRDEDNAGYRKVM
                     RDTLEFDPGVLGRFVNFLTNPDEKPAAEQLGTGDKSFSAATLLATLPGLPMLGHGQVE
                     GFRERYGMEFSRARWDEPIDEAHAGHFEWAIVPLLRRRSAFSGTDRFHLYDAHGDGGG
                     VVEDVYAFSNGTGDTRSLVLVHHRHADTTVRIDWSVAAAPPGGGSGRSSVRLADALEL
                     AGPDDALVYLRDARSGWELVRTAGELRREGLRVTLGPYEARVLSIELDAAIAQATPPA
                     EVGSPKPPPRKRAATTSTKRAKPAKPAKPTPRAKPKKPASRGTGTAGR"
BASE COUNT         1238 a         2628 c         2642 g         1173 t
ORIGIN      
        1 gaccgacgcc caaataaagg tgctcatcga cgccggcgag ttcgagagcg tcggagcgca
       61 gaccggggac tatctgggcg tgccgctcaa gaacgaatcg agaaccgtcg gcgtccttgc
      121 cgtccagagc tacgtcgagg gcatcggcta tgaccaggag gacgagcgcc tcctcgcgta
      181 cgtcgcccgg cacatcgccg cggctctcga gcgaacgcgc gcaacggccg agatccgtca
      241 gcggaacgcc gagctcgcgc tcatcaacga ggtcggcgat gcgctcgcga aacagctcga
      301 cttccacgcg atcgtcgagc tcatcggcga gcgcctggcg aggatgttca actcgctgga
      361 cgtcttcatc gcgatgtacg agaaggttgc ccagcagatc accttcccgt acgagctcga
      421 ccagggccgc cgcgtccaca gtgatccaat tccctttggt cccggcctga cgagcgaggt
      481 cctgcggacc ggccggccgc tcgtattcgg gaccgccgcc gagcaggaga tccacagccc
      541 cgttaccact gcgttcgccg agggtggcat ggcctccgag gctggctcat ggctcggggt
      601 accgatcatg agcggcgacg aggcgatcgg ggtcatcgcg ttctccgaat accgggagaa
      661 cgcgttcacc gacgccgacg tccgcctcgt caccacgatc gtgacgagca tgggcgttgc
      721 actcgagaat gcgcgactgt tcgagcagac caacacgctg ctcgccgaga cgaaggagcg
      781 agccgctgag ctcgcgatca tcaacagtgt tcaggagggc ctcgcggcca agctcgagac
      841 ccaggccatg tacgacctcg tcggcgacaa gatccgggag atcttcgacg cccaggtcgt
      901 cgacatcggc attctcgatc gcgagaacgg catcttccat ttcccgtact cgatcgagcg
      961 cggcgtccgc ttcccggacg agccgatggg gatcgtgggc taccggaaag tggtgttcga
     1021 gaccaaggcg ccgttgctcc tcaatcgagt ctcggtgagg tttgccgccg aggtcggtca
     1081 gggcgcggcg atccagggcg aggatccgct gtcgctgctg tttgccccgc tcgtcaccgg
     1141 cggtgacgtg accggcgtga tctcgctcca gaacctcgac cacgaggacg ccttcagcga
     1201 cagcgacgtt cgcctgctga ccacgctcgc gggcaccctg agtggggccc tcgagaacgt
     1261 ccgcctgttc gacgagacaa agcgactcct cgccgagagc accgagcgcg ccgccgagct
     1321 cgagatcatc aacagcatcc agcagggcct cgcgggtcgg ctcgagatgc aggcgatgta
     1381 cgacctggtc ggcgagaaga tcgcggagat cttcgacgtc cacggtgtcg acatcgagtc
     1441 ctacgaccgg tcgaccgaga tgatccactt cgcctacacg gtggagcgtg gcgagcgcct
     1501 tgctgccgat ccgatcccgc tcttcgggtt ccggcgtcag gtcgtcgaaa cgcgggcacc
     1561 cgtggtcgtg aatcgcgacc tcgaagctcg agccgccgag gtcgggcagc ccgccaccat
     1621 cgccggcgag ttggccaagt cggtcatctt cgtgccgatg atcatcgccc gcgaggtcac
     1681 cgggatcatt ctcatcgaga acctggagcg tgaggatgcg ttcagcgacg gcgacgtccg
     1741 cctgttgacc acgctcgccg caagcctgag cgtcgccctc gagaatgcgc gcctcgtcgc
     1801 cgagacacgc cagcgcgcaa gtgagctgtt gatcgtcaac gaggtcggcc aggcggcggc
     1861 ctcccagctc gatcttgatc ggctcatcca gctcaccggg gagcagctcc ggacgacgtt
     1921 ccgcgccgac atcgtctaca tcgcattgct caacgacgac accggcctga tcgatttccc
     1981 gtaccgcacc gagcgcggca aggtgacccc gcgtccaccg atgcgggtcg gcgaaggcct
     2041 gacctcgcag atcctttcgt cacgcgagcc cttgctgctc aatcgcgcgg accaattctc
     2101 ggtgatggag cagaaggttg ggacggcggt gaagtcctac ctcggcgtgc cgatcctggt
     2161 cggcgaccgg gcgatcggtg cggtcagcgt ccagagcatc gacgaggccg gccggttcgg
     2221 cgaaacggat gcgcgcgtgc tctcgaccat cgcgtccaac gtcgggactg cgatccggaa
     2281 cgcccagctc taccgcgagg cgcagcgccg ccagcaggag accgcggccc tgaacgaggt
     2341 cgggcgggag atttcggcca cgctcgacct cgaggtcgtc ctcggacgaa tcgccgaacg
     2401 ggcgcaggtc ctgtcggggg ccgccacgag cgccgtcttc cttgtccagc ctgacggcag
     2461 gacgttgaag gccatcaccg tggtcggcga agccgtcgac gagatcaagg cagacgagtt
     2521 caccctgggt gaagggatca tcggcaccgc ggcagcggag cgccgaccgg aaatcgtcaa
     2581 tgacgtgctc cgagaccccc gcagcgtcac gatcgccggc acgttcgagg acgacgagga
     2641 ggaagagcgg ctcatgtctg cgccgctgct ggggcggcag ggactcagcg ggatgatggc
     2701 ggtctggcgc gctggcgcgg cgccggcgtt cacgcaggca gacctcgact tcctcgtcgg
     2761 gctttcccag caggcagcaa tcgcgatcga caacgcccgc ctcttcgccg acgcggacgt
     2821 ggcccgcaac gccgccgacg aggcgaacca ggccaagagc gccttcctgg ccgcgatgag
     2881 ccatgagatc cggaccccga tgaacgcgat catcggcatg agcgggctgc tgctcgagac
     2941 gacgctggat gaggagcagc gcgacttcgc cgacacgatc cggacttcgg gcgatgcgct
     3001 cctcacgatc atcaacgaca tcctcgactt ctcgaagatc gaggccggca aggtcgatct
     3061 cgtccacgag ccgttcgcgc tcgctgcgag catcgagggc gcactcgacc tgatcgcgcc
     3121 gaccgccggg aagaagggca tcgagctcgc ctacgaggtc gctggtgacc tgccgcccgc
     3181 cgtcgcgggc gaccagggcc ggctgcgcca gatcgtgctc aatctcctct cgaacgcggt
     3241 caagttcacc gattcgggcg aggtcgtcgt ctccgtccgg agctcgccgt tcggggcgag
     3301 gtgggaggtc gcggtcgagg tccgggacac cggcatcggc atcccggccg accgcatcgg
     3361 gcgcctcttc cagtcattca cccaggcgga ctcgacgatc tcccggcgat tcggcggcac
     3421 cggtctcggg ctcgccatca gccggcggct tgccgaggcg atggacggca gcctcgtcgc
     3481 ggagagctcc ggcgtcgcgg gcgagggcac gacgttcaag ctcctcgtcc ggctggatgc
     3541 ggcggaggcg tcggccgtgc ccgagcgccg cgatcgcgat ctcgtcgaga tcgccggtcg
     3601 caccgccctc atcgtcgatg acaacgcgac gaaccggcga attctgacgg cccagctcgc
     3661 gcgctggtcc atgacgacgc gcgagaccgc atcggccgcg gaggcgctcg cctggatccg
     3721 tgcgggcgag cagttcgacg tggtcctgct cgacctgcag atgccggaga tggacgggct
     3781 cgcgctcgcc gacgccatcc gagcgatcga gcagccgacc cagccgaagt tgatcctggt
     3841 gtcgtccatc ggcattcgcg agcgcggcaa tgcggcattg gacgcggtcc tcaccaagcc
     3901 catcaagccg tccgcgctcc acgatgccct cgtcaacgtg ctgtcggtgg gtggcggtgc
     3961 cggggaggat cggctccagc gtgcgcccga gcgcccggcc gtggatccgg agctcgccca
     4021 gcgtcatccg ctgcgcatcc tcctcgccga ggacaacgcg gtgaaccgca aggtcgccca
     4081 gcggctcctc gccaacatgg gctatgccgc ggacatggcc gagaacgggc tgctcgcgct
     4141 cgaagcgatt gaagcgaagg agttcgacgt cgtgctcatg gacgtgcaga tgccggagat
     4201 ggacggcctc gaggcgacac gacagatccg ggcccgttgg cccgagcggc ccatccatat
     4261 cgtggcgatg accgcgaacg cgatggccgg cgaccgcgac ctgtgcctcg ccgccggcat
     4321 gaacgactac gtgagcaagc cgatccgggt cccggagctg gccgcagcgc tcgctcggac
     4381 accgagcctc gccgacgtga cccgatgacg gccgacgacg caccgatcct cgacgacggg
     4441 gtgatcgcgg agctccgcgc atcgaccggc gacgatgacg acttcgtgcg cgagctggtc
     4501 gaggcgtaca tcgaggaggg ccgcggctac atcgcggcga tgaacaccgc cgccgcagcc
     4561 gccgacgccg aggcgatggt tcgcccggcc cacacgctca agtccagcag cgcgacgatg
     4621 ggcgcgatgc gcctggcagc aatctgccgc gggatcgaag aggccgcccg tgacggcgag
     4681 aacgaaggcc tcgcggccga tgtcgatgcg gccactgcgg cgtggtcggc aacgctcgag
     4741 gccatggccg cggcagggct cacggcgtga acgcgtcgcg ggcggtcgcc ctggtcatcg
     4801 acgacagcct ggtcaatcgc aagctcctga cacggctgct cgccaccctc gatatcgagg
     4861 cccgcgaggc cgccaacgga cgtaccgggc tcgagctgct ccgctcgtcc gaggctgacg
     4921 tggcggtcgt cctgctggac atcgtcatgc ccgagatgga cggctacgag acgctcgccg
     4981 cgatcaaggc cgacgagtcc ctgcggcacc tgccggtgat catgatctcg ggcatcgagg
     5041 agctcgacag cgtcgtccgg tgcatcgagc tgggagcgac cgactacctc ccgaagccgt
     5101 tcgatgccgt gatcctcggc gcgcgcatcc gggcttcgct cgccggcaag cagctccacg
     5161 acctccagat cgaatccgag cgccgccagg ccgagctcct ccgaacgatc aaggcgcaga
     5221 aggaggaact gagccgcttc ttctcgccgc aggtcgccgc gctggtatcc agccccgaag
     5281 gcgtccagat gctcgccggt caccgccgcg tcgcgacatc cctctttgcc gatcttcgcg
     5341 gcttcacgcc gttcagcgag gccaccgatc ccgaggtggt gctggacgtg cttcgcgagt
     5401 accacgttgc gatgggcgcc ctgatcgtcg ccgcgggcgg cacggtcgag cacttcgccg
     5461 gcgacggtat gcacgtgttc ttcaacgatc cgatcgtcca ggacgaccac gtggagcgcg
     5521 ccgtccgggt cgcggtcgac atgcgcgacc ggttcgccga gctcgacgcg ggctggactg
     5581 cgcgggggta cacgctgggc ttcggagtcg gaatcgccac cggctttgcc accctcgggc
     5641 gcatcggctg ggagggtcgc tacgactacg gcatggtcgg cacggccgtg atcgtcgcgg
     5701 cacggctgag ctcggtcgcg ctcgccaacc aggtgctcct gagcgaggag tcctacgcgg
     5761 aggtccggga cctcgtggac gtggagccgg tcggggagct gcacctgaag ggcttcagtc
     5821 gcccgatcgt gccgctcaac gtcctgcggt tccggtcccc cgactcgccg gcttcttcgg
     5881 cttcgctcgc ggcgttggct tcgccggctt tgccggcttt gcccgcttcg tcgacgtagt
     5941 cgccgctcgc tttctgggcg gcggcttcgg gctcccgacc tcggcggggg gcgtcgcctg
     6001 cgctatcgcc gcgtcgagct cgatcgacag gacccgtgcc tcgtacgggc ccagcgtcac
     6061 gcgcaggccc tcccgccgca gctcgcccgc ggtcctgacg agctcccagc cggagcgtgc
     6121 gtcacgcagg tagacgagcg cgtcgtcggg gcccgcgagc tcgagcgcgt cggcgagacg
     6181 gacgctcgac cgccccgagc cgccaccggg tggcgcggcg gccaccgacc agtcgatgcg
     6241 caccgtcgtg tccgcgtgcc ggtgatggac gagcacgagc gagcgggtgt cgccggtccc
     6301 gttcgagaag gcgtagacgt cctcgacgac gcccccgcca tcgccgtgcg cgtcgtacag
     6361 gtggaaccga tccgtgcccg agaacgccga ccgccggcgc aggagcggca cgattgccca
     6421 ttcgaaatgc ccggcgtgcg cttcgtcgat cggctcgtcc cagcgggccc ggctgaactc
     6481 catcccatag cgctcgcgga agccctcgac ctggccgtgg ccgagcatcg gcaggccggg
     6541 cagggtcgca agcagcgtcg ccgccgagaa cgacttgtca cccgtcccga gctgctcggc
     6601 ggcgggtttc tcgtcggggt tggtcaggaa gttcacgaac cggccgagca cgcccggatc
     6661 gaactcgagc gtgtcccgca tgaccttgcg gtagccagcg ttgtcctcgt cgcgaagcat
     6721 gtgcatgaac gccgagttgt agacgcggtg catgccgagt gtccggacga agtagccctc
     6781 gaggagccag aacgcttcgg cgagcagcag cgtccccggc gcctcggcgg cgacgcgatc
     6841 gacgacctcg cgccagaact cggcgggcat gcgccggttg aactccgcgt tcgagagggc
     6901 gtgctcggcg cgtgacggga tcgcgccgcc cgcgcccggc tccgggtacc agaggcggcg
     6961 gatgtgccgg cgcgcgagca ccatcgcggc gtcgaagcgc aggatcggcg cccgccgcgc
     7021 gacgtcgagg atcgtctgga tgacggcttc acggacatcg gcgcggaggt agtccagctg
     7081 ggcggtgtcg ttccacgggt acgaggtgcc gtcgttgccg tggtagatga agcgctgctc
     7141 gcccgaggcg gtatcgcgcc gcttgaagac gaccgcggcg tcgctgccgt cccagtagtg
     7201 atcctcgatc tggaggtcga cgcgcggatc gggtgacagg ttcggcccgc cgaaggtgta
     7261 ggcgggaaag ggcggctcgt tgaccgacag gaaccgctct gggtgctcga tgacccagcg
     7321 cgaatcgatg cccatgtggt tcgggaccat gtcactggcg aggcggatgc cgcgagtcca
     7381 ggcccggtcg cgcaggtccg cgtaggcgcc ctcgccaccg aggtcgtcgg cgatccggta
     7441 gtcgtcgagg gagtaggccg acgcgaccgc atccgcgttg ccgcgcatcc gcttgatccg
     7501 ctccgaggcg atggaccgtt cccacaggcc gatgagccac aggccggtga cgccccagcg
     7561 ggcgagggcg tccagttcgt catcggggat ggcgtcgagc gtccggatgt cgcgccggta
     7621 ggtccgggac aactggtcga gccagacata ggtgctcttg gcgatcatca cgacgcgcgg
     7681 c
//
LOCUS       DATBSW010000205         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_794962, whole genome shotgun sequence.
ACCESSION   DATBSW010000205 DATBSW010000000
VERSION     DATBSW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794962"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(685..1020)
                     /locus_tag="VIF63_05730"
     CDS             complement(685..1020)
                     /locus_tag="VIF63_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238917.1"
                     /translation="MPIDLVATIVPSPPPAQEELFPARCSVFGEKVAIAFSLATTPFT
                     VPAGSQEIPGFAHNAYLEKLTAGNWTLWVPLTADLGYLFVEVNNQDGKDHTDEAVNLA
                     KAILQKLGG"
     gene            complement(1276..>2621)
                     /locus_tag="VIF63_05735"
     CDS             complement(1276..>2621)
                     /locus_tag="VIF63_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238918.1"
                     /translation="TQPTSLFDYEAGTTTDSYTTPGFPEESAPLSVEDLSPEVIAAAR
                     TTCAAVTDPGLADECAFDVATTGSTEYVTLYSLSDDFQAQGPVALGAAPAPTPTPRGS
                     GGPSAGIVLVGDHLTGAADARLGPDGTVYVEVGEQPIAFGDVTPVLLALDGATGVVKH
                     RAVAVAGGRLVWAAGALWAAEFQRGDFGCQVSRLDPTTLAPQASIPTVCYDGQTVLVT
                     LDDAPWFIDPTGAGSNGEGAHLQRIDPTTNKLDANPDGRIELPFASSALGRIGFGSVL
                     ATTSAGVIFGERHDGIFRINLDGSVDPLGSPGAFSAFFAAGNGVWTQTVIGGFEDPSG
                     TAEFFTGGSASELRVGIVGDLIGADESAVYGSYAEFDDEPDGFWRFPLDGGAPQQLAG
                     SGFTPNGFGGQLRLLYRDITVPLLIGDGLAVKLWIAPSPTNDTQSALLFQSIPLP"
BASE COUNT          405 a          886 c          921 g          409 t
ORIGIN      
        1 caccgggagt tgtggcacgt aacggtgtcg ccgggtcacc gtggtgtcgc cgggtcaccg
       61 tggagccgcc gggtcaccgt ggagccgccg agtcaccgtg gagttggcgc gcgagctcct
      121 cgagtcgatc ttggcgacgc cgctcacggg ctgcgcggcg ttcgcctccc gcgctgggtg
      181 agaacgcgtg gcgcatcaat cgcagcgcgt gcgaacgctc gacaggcgta cgagcgtccg
      241 gaaccgtgtg gaccttcatg tcgagcccga cgaggagccg agcgacccac gcgacccgga
      301 cgccatccgc gagcccgcac tcgagcctcg agatggtcgc ttgcgacaga ccgcaccgtc
      361 cggcgagcgc ccgctgcgaa agaccgcgcg acccgcgcag gtcccgcagg acttgaccga
      421 gcacctggag tgcctctcgc gtttcgtagt cgatctgtcc gaggtcgtgc ggcatacacg
      481 atttgaccac cgacgccttg gcggccgctt acgcgcgtgg ttgattaccc cataggcatc
      541 acggaccacg tgagaaacat ttcggccacc gcgttcgatc cgcgtgcgac gcccgagcgt
      601 ttgcggtacc agctcgcgaa cggcgtcgcc ttgcgtggcg ccgcctgcgc taacagctag
      661 acgctactcg ctacgcgcta cgcgctagcc gccgagcttc tgcaggattg ccttggccag
      721 gttgacggcc tcgtcggtgt gatccttgcc gtcctggttg ttgacctcga cgaagaggta
      781 gccgaggtcg gctgtcagcg ggacccacag cgtccagttg cccgcagtga gcttctcgag
      841 gtaggcgttg tgggcgaacc cagggatctc ctgggagccg gctggcaccg tgaacggcgt
      901 ggtggcgagc gagaaggcga tcgcgacctt ctccccgaag acgctgcacc ttgccgggaa
      961 cagctcttcc tgtgccggcg gcgggctggg cacgatcgtg gccacgaggt ctatcggcat
     1021 gagctcgcag gcatgggcca acgcggcggt agcggcttcg aggattccgg cgccgccgtc
     1081 acccgcactc gtagcctggc ccgcgctggt gggctggccg gcgctcgtgg cgccaggacc
     1141 ggcggtctgg cctggtccac ccgtggagca tgccgcgacc aggactgtca agacgattgc
     1201 cgcaccgatg cgtcgcatgg cgacccccga ctacgagctc gacgcgcgga ttgtcctcca
     1261 atgccggatt cgtgcctagg gcaatgggat cgactggaac agcaaggctg attgggtgtc
     1321 gttggtcggc gatggcgcga tccacagctt gaccgccagg ccgtcgccga tgagcagcgg
     1381 taccgtgatg tcgcggtaca gcaggcgcag ctgaccgccg aacccgttcg gcgtgaagcc
     1441 ggaccccgcg agctgctgcg gtgcgccgcc atcgagcggg aagcgccaga agccgtcggg
     1501 ctcgtcatcg aactcggcat agctgccgta gaccgccgac tcgtccgcac cgatgagatc
     1561 gccgacgatc ccgacccgca gctccgatgc cgacccgccc gtgaagaact ccgcggtgcc
     1621 gctcggatcc tcgaatccgc cgatcaccgt ctgcgtccaa acgccgttgc cggcagcgaa
     1681 gaaggcggag aacgcccccg gagacccgag cggatcgacg ctgccgtcca aattgattcg
     1741 gaatatccca tcgtggcgct caccgaagat cacgccagcc gaggtggttg cgagcaccga
     1801 cccgaagccg atccgtccga gcgccgagct cgcgaacggc agctcgatcc tgccgtccgg
     1861 gttcgcatcg agcttgttcg tcgtggggtc gatgcgctgg aggtgggcgc cctcgccgtt
     1921 cgatccggcc ccggtgggat cgatgaacca gggcgcgtcg tcgagcgtga cgaggaccgt
     1981 ctggccgtcg tagcagacgg tcgggatgct cgcttgcggc gcgagcgtcg tcggatcgag
     2041 gcggctcacc tggcagccga agtcgccgcg ctggaactcg gcggcccaga gcgcccccgc
     2101 ggcccagacg agccggcccc cggcaacggc gaccgcgcga tgcttcacga cgcccgtcgc
     2161 gccgtcgagc gcgaggagga ccggcgtgac gtcgccaaac gcgatcggct gctcgccgac
     2221 ctcgacgtac accgtgccgt cggggccgag gcgcgcgtcc gcggcaccgg tcaggtgatc
     2281 gccgacgagg acgatgccgg ccgatgggcc cccgctgccg cgtggtgtcg gcgtcggggc
     2341 aggggcggct ccgagtgcga cgggtccctg ggcctggaaa tcgtccgaga gcgagtagag
     2401 ggtcacgtac tccgtggagc cggtcgtggc cacgtcgaat gcgcactcgt cagcgaggcc
     2461 cggatccgtg accgcggcac acgtcgtgcg agccgccgcg atgacttccg gtgagaggtc
     2521 ctcgacggac agcggcgccg attcctcggg gaagcctggc gttgtgtacg aatccgtggt
     2581 cgtgcccgct tcgtagtcga acaggctcgt tggctgggtc a
//
LOCUS       DATBSW010000206         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_230783, whole genome shotgun sequence.
ACCESSION   DATBSW010000206 DATBSW010000000
VERSION     DATBSW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230783"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..650
                     /locus_tag="VIF63_05740"
     CDS             144..650
                     /locus_tag="VIF63_05740"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8238919.1"
                     /translation="MTEASGTTEASGTVETSGTTEKRPKLPPGWFIRIAWAVHRAAYR
                     WTGGRRGLWRATPEKWGTFRLHTIGRKSGKERIAILGYHEDGPNIVTVAMNGWMEGDP
                     AWWVNLQAKPDATIELKGKTLRVRARAAAAEEREHLWSLFPDAQAFVKHRKTETAIVV
                     LEPMSDTG"
     gene            complement(940..1152)
                     /locus_tag="VIF63_05745"
     CDS             complement(940..1152)
                     /locus_tag="VIF63_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238920.1"
                     /translation="MTDSRTPRPDRTRYVLAVLVGLVGLVWVAQGLGAPIGGGFMVGD
                     VFWAYAGAVLVALAVGYAALPRLRRR"
     gene            1203..2069
                     /locus_tag="VIF63_05750"
     CDS             1203..2069
                     /locus_tag="VIF63_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8238921.1"
                     /translation="MGRDDVRGKPEGRAPARAGAAPDPDVSGEPIGEHRRSGRLVMGK
                     DMARKMPGDATMTEDKKLLERTTRPAFLDTDTWRALRILSEFVEGFDALAQIGPAITV
                     FGSARVSEGNPAYDQAREIGRLLALEGYAVITGGGPGVMEAANRGCQEGGGLSVGCNI
                     ELPHEQDINGYVDLGVQFRYFFARKTMFVKYADGFVILPGGYGTLDELMEALTLIQTG
                     KIEHFPVVLVGTQFFGGFVDWIRGTLLSEGMIAAEDIDLIQVTDDPKQVVEIIRSVRR
                     NRPGPATDTAIG"
     gene            complement(2066..2563)
                     /locus_tag="VIF63_05755"
     CDS             complement(2066..2563)
                     /locus_tag="VIF63_05755"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8238922.1"
                     /translation="MTVFDSEPKIAPIPRGLTASGTAAASFEPALALAELPSGAMRRV
                     TWGDVDILLAHTPSGIVAVDDRCPHMSAPLSIGTLDGCLIDCPLHSGQFDLETGDATR
                     MPTTGGLDADGQYHPVWSPEGREPKPDPPGIKAEARRYTRVRRIRYYPVRLRDGTIEV
                     AVPQA"
     gene            complement(2841..>3078)
                     /locus_tag="VIF63_05760"
     CDS             complement(2841..>3078)
                     /locus_tag="VIF63_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238923.1"
                     /translation="FDFVKLLPPDVAALVLDGFHRALTISIGNSIWLGVAASVVALGT
                     AFFLKEIPLRTTRGATSAATAGARGRAQPTPAAD"
BASE COUNT          521 a         1018 c         1109 g          430 t
ORIGIN      
        1 cggtcaccga ggaagtcgag cgcctgtcgg cgatcctcga tcggtaaggc catcgagggc
       61 ggcaaggctt cacgacatgt tcacaacacg gccggagact gcccgtatcg tggtcgccct
      121 ggccgcgcag acgggagaca gcgatgaccg aggcgagcgg aaccaccgag gcgagcggga
      181 ccgtcgagac gagcgggacc accgagaagc ggccgaagct gccgccgggc tggttcatcc
      241 gcatcgcctg ggcagtgcat cgggcggcct atcgctggac cggtggccgg cgcgggctgt
      301 ggcgcgccac ccccgagaag tgggggacct tccgactcca cacgatcggg cgcaagtcgg
      361 gcaaggagcg gatcgcgatc ctcggctacc acgaggacgg cccgaacatc gtcaccgtcg
      421 cgatgaatgg ctggatggag ggcgaccccg cgtggtgggt gaacctgcag gccaagcccg
      481 acgcgacgat cgagctcaag ggcaagacac tgcgggtccg agcacgagct gccgccgccg
      541 aggaacgtga gcacctgtgg tcgctgttcc cggacgcgca ggccttcgtg aagcaccgca
      601 agaccgagac ggcgatcgtg gtcctcgagc cgatgtcgga cacgggctag gcgcatcctg
      661 ccgcagccgt gcggtggcgg atctcgaggc gaatcggatg gccgtgatcg cgtagcgatc
      721 atttctggcg tttggggcgc ccattgctgg aatccaaggc cccggaggtc ggtctgggct
      781 caaggtttga gcacgattga acgtggactg gagcttggaa gcggatcgag ccggccctgc
      841 aggcctgaga tggccgtcga agcccggaaa atgatcacat cgtgatcatc cacgtcggtt
      901 tgtcgccctc tgggcaccgt cgaatcgcgg aggctgccct cagcggcgcc gcagccgagg
      961 cagggcggcg taccccacgg ccagagccac gaggaccgcg cccgcgtagg cccagaagac
     1021 gtcgccgacc atgaagccgc cgccgatcgg cgcgccgagg ccctgggcga cccagacgag
     1081 gccgacgaga ccaaccagca ccgccagaac gtagcgggtc cggtcggggc ggggcgtgcg
     1141 ggagtcggtc accgtcgtag gatgcgccga tcggcgctga gccgcccccg aggaggcaat
     1201 cgatgggtcg agacgacgtc cgcggcaagc cggagggccg cgcgcccgca cgggccggcg
     1261 ccgctccgga ccccgacgtg agcggcgagc cgatcggcga gcaccgccgc tcgggccggc
     1321 tggtgatggg caaggacatg gcccgcaaga tgccgggcga cgcgaccatg accgaggaca
     1381 agaagctcct cgagcgcacg acgcggccag ccttcctcga cacggacacg tggcgcgccc
     1441 tgcgcatcct gtccgagttc gtcgagggct tcgacgccct cgcccagatc ggtcccgcaa
     1501 tcacggtctt cggctcggcg cgcgtgtccg agggcaaccc cgcgtacgac caggctcgtg
     1561 agatcgggcg tttgctcgcc ctcgagggct acgcggtgat caccggcggc gggccaggcg
     1621 tgatggaggc cgcgaatcga ggctgccagg agggcggcgg gctctcggtc gggtgcaaca
     1681 tcgagctgcc ccacgagcag gacatcaacg ggtacgtcga tctcggcgtg cagttccggt
     1741 acttcttcgc gcgcaagacg atgttcgtga agtacgccga cggattcgtg atcctgcccg
     1801 gcggttacgg gacgctcgac gagctcatgg aggcgctgac gctcatccag accggcaaga
     1861 tcgagcactt tccggtggtc ctggtcggca cgcagttctt cggcggcttc gtcgactgga
     1921 tccggggcac gctgctgagc gagggcatga tcgcggccga ggacatcgac ctcatccagg
     1981 tgaccgacga tccgaagcag gtcgtcgaga tcatccggtc cgtccgccgg aaccgacccg
     2041 gtccagccac cgacaccgcg atcggctagg cctgcgggac ggcgacctcg atcgtcccgt
     2101 cgcggaggcg aacggggtag tagcggatcc ggcgcacgcg ggtgtagcgg cgcgcctcgg
     2161 ccttgatccc gggcgggtcc ggcttgggct cgcgaccttc cggtgaccag accgggtggt
     2221 actgcccgtc cgcgtcgagg ccgcccgtcg tcggcattcg ggtcgcgtca ccggtctcga
     2281 ggtcgaactg gccgctgtgc agcgggcaat cgatcaggca gccgtcgagc gtgccgatcg
     2341 acagcggcgc cgacatgtgc ggacaccggt catcgacggc cacgattccg ctcggggtat
     2401 gcgcgagcag gatgtcgaca tcgccccagg tcacccggcg catcgcgccc gagggcagct
     2461 ccgcgagggc gagcgcgggc tcgaagctcg cggccgccgt gccggacgcg gtgagcccgc
     2521 gtgggatcgg cgcgatcttc ggctcagaat cgaagacggt catcgtccga gcctagcccg
     2581 aattgcacag atccccgccg agccgacgct gtttcggacc ttgatcctgt cgaatgacac
     2641 ggtgtgtagc gctcggcaag gcggggcccg acgctccctc gccatctgac acgaattggc
     2701 accgtacgtg tcgctcaggc tgcatccggt gccaattgac gctgttccgg gccgcgacag
     2761 acgcgtacgg gcggccgaag cccgtcgaac gctaccggcg gtagcgttcg acagaaagac
     2821 cgatcaggcc ggattcgccg tcagtcggcc gcgggcgtcg gctgggcccg gcctcgagca
     2881 cccgcggtcg ccgcgctcgt ggcaccgcgg gtggttcgaa gcgggatttc cttcaggaag
     2941 aaggcggtcc cgagcgccac gaccgaagcc gcaacgccga gccagatgct gttgccgatc
     3001 gagatcgtca gcgcccggtg aaagccgtcg aggacgagtg ccgcaacgtc cggcgggagg
     3061 agcttcacga agtcaaac
//
LOCUS       DATBSW010000207         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_795055, whole genome shotgun sequence.
ACCESSION   DATBSW010000207 DATBSW010000000
VERSION     DATBSW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795055"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1844
                     /locus_tag="VIF63_05765"
     CDS             <1..>1844
                     /locus_tag="VIF63_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8238924.1"
                     /translation="LGASSTLTLLNGRRIAPYGLADDGQKVFTDMSSIPMSAVERIEV
                     LLDGASAIYGSDAIAGVVNIIMRKDFQGGEAQVSYGISEEGDGDSLKASAILGFGDLS
                     RDKYNVFFNLDYMKADKIKLSDRLGRGPVGAYDYRAYDMDPHGSQWIAGYITGGVATS
                     MPTGALRDPATGLYTQLPGCAQIQPVQIPALDSEGGCLWHVGQFRDMLPEMEGISFFG
                     RGTLALSPTMEAYGEIGYSKRETDFFTNPTGSSGSWGYPGGPVNASSGPGAIVLAASH
                     PDNIFGVGNVRLRYSAWDVGPRTSHTDTEFTRLSAGLKGSGWGWDFDTGFVWSKSETT
                     NTRNGFFRYSILRAALGDPTSPYFPYRIGVNANLNTPAQYAALSPTIQSQADSEMSLV
                     DFKGSKELMQLDGGPMSLAAGIEYRKVDQSLKPTTYTDQGDILGLGYSAYSGSQTVMA
                     IYGELLAPVHKMVELSAALRYDNYKDGEDSTTPKLGIKFMPIRELVVRGTYAEGFRQP
                     NVAESGEGGLAAFTTARDPVRCPGGTPAPGASAGNCNQSVAIITTPEPSLKPEQSKNY
                     SIGFIWEPLQSTSFSMDFWRIKRTDEINQTTVGAALAAGTNVVRGDDN"
BASE COUNT          327 a          656 c          582 g          279 t
ORIGIN      
        1 ctcggcgcga gctcgaccct gaccctcctg aacggccgtc gcatcgcccc ctacggcctc
       61 gcggacgacg gacagaaggt cttcactgac atgtcctcga tcccgatgtc cgccgtcgag
      121 cgcatcgagg tcctgctgga cggcgcctcg gcgatctacg gctcggacgc catcgcgggc
      181 gtggtcaaca tcatcatgcg caaggacttc cagggcggcg aagcccaggt gtcctatgga
      241 atttcggaag aaggcgacgg ggactcgctc aaggcctcgg ccatcctggg cttcggcgac
      301 ctgtcgcgcg acaagtacaa cgtcttcttc aacctcgact acatgaaggc cgacaagatc
      361 aagctgtccg atcgcctcgg ccgcggcccc gtcggcgcct acgattaccg cgcatacgac
      421 atggatcccc atggcagcca gtggatcgcc ggctacatca cgggcggcgt ggcgacctcc
      481 atgcccaccg gcgcgctgcg cgaccccgcc acgggcctct acacccagct tcccggctgc
      541 gcgcaaatcc agccggtgca gatcccggcg ctcgattccg aaggcggctg cctctggcac
      601 gtcggccagt tccgcgacat gttgccggag atggaaggca tcagcttctt cggccgcggc
      661 accctggcgt tgtcgccgac gatggaagcg tacggcgaga tcggctactc gaagcgcgag
      721 accgacttct tcaccaaccc gacgggttcg tcgggctcct ggggctaccc gggcggcccg
      781 gtgaacgcga gcagcggccc gggcgcgatc gtgctggcgg cgagccaccc ggacaacatc
      841 ttcggcgtgg gcaacgttcg cctgcgctac tccgcgtggg acgtgggtcc gcgcacctcg
      901 cacaccgaca ccgagttcac gcgcctgtcc gcgggcttga agggctcggg ctggggctgg
      961 gacttcgaca ccggcttcgt atggtccaag agcgagacca ccaacacccg caatggcttc
     1021 ttccgctaca gcatcctgcg cgcggcgctg ggtgatccca ccagcccgta tttcccgtac
     1081 cgcatcggcg tgaacgcgaa cctcaacacg cccgcgcagt atgcggcact gtcgcctacg
     1141 atccagtcgc aggccgactc ggagatgagc ctcgtcgact tcaagggctc gaaggagctg
     1201 atgcagctcg acggcggccc gatgagcctt gcggcgggca tcgagtatcg caaggtcgac
     1261 cagagcctca agccgaccac gtacacggac cagggcgaca tcctgggcct gggctactcc
     1321 gcctatagcg gctcccagac cgtgatggcc atctacggcg agctgctcgc gccggtccac
     1381 aagatggtcg agctgtcggc cgccctgcgc tacgacaact acaaggacgg cgaggactcc
     1441 acgacgccca agctcggcat caagttcatg ccgatccgcg agctcgtggt ccgcggcacg
     1501 tacgccgagg gcttccgcca gccgaacgtg gcggagtcgg gcgaaggcgg cctggccgcc
     1561 ttcaccaccg cgcgcgaccc ggtccgatgc cctggcggca cccccgcccc gggcgcgagc
     1621 gccggcaact gcaaccagtc ggtcgccatc atcacgacgc ccgagccgag cctcaagccc
     1681 gagcagtcca agaactacag catcggcttc atctgggagc cgctgcagtc aaccagcttc
     1741 tcgatggact tctggcggat caagcgcacc gacgagatca accagacgac ggtcggggcg
     1801 gcgctggctg cgggaacgaa cgtggtgcga ggcgacgaca actt
//
LOCUS       DATBSW010000208         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_724663, whole genome shotgun sequence.
ACCESSION   DATBSW010000208 DATBSW010000000
VERSION     DATBSW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724663"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /gene="tuf"
                     /locus_tag="VIF63_05770"
     CDS             <1..132
                     /gene="tuf"
                     /locus_tag="VIF63_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY8238925.1"
                     /translation="MPGDNVEMGVELITPIALEVGQRFAIREGGRTVGAGAITSISA"
     gene            137..301
                     /gene="rpmG"
                     /locus_tag="VIF63_05775"
     CDS             137..301
                     /gene="rpmG"
                     /locus_tag="VIF63_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY8238926.1"
                     /translation="MARVEARPTLQLACTECKERTYTTRKNKKNDPNRIELNKYCPRC
                     RKHTLHREAK"
     gene            317..392
                     /locus_tag="VIF63_05780"
     tRNA            317..392
                     /locus_tag="VIF63_05780"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Trp,seq:cca)
     gene            420..617
                     /gene="secE"
                     /locus_tag="VIF63_05785"
     CDS             420..617
                     /gene="secE"
                     /locus_tag="VIF63_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY8238927.1"
                     /translation="MKRIRRFIAEAVSELRKVSWPTFVQTRNLTVLVFAVSIAVGVFV
                     AIADQLFQAVIGTVAVFLGGS"
     gene            619..1200
                     /gene="nusG"
                     /locus_tag="VIF63_05790"
     CDS             619..1200
                     /gene="nusG"
                     /locus_tag="VIF63_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY8238928.1"
                     /translation="MSTTVTTDIEAKRPDKRWYVIHTYSGYENKVKANLEHRIESMGM
                     EDKIFQVLVPMEDEIEIRQGQRHTVQKKVYPGYVLVEMILDPDSWFVVRNTPGVTSFV
                     GGSNIPGVRSEPVPLDETEVKQILRHMGVETPKYRVAFTKGQSVRVTDGPFSEFIGTV
                     DEVNPERNKVKVLVSIFGRETPVELDFLQVEKL"
     gene            1219..1644
                     /gene="rplK"
                     /locus_tag="VIF63_05795"
     CDS             1219..1644
                     /gene="rplK"
                     /locus_tag="VIF63_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY8238929.1"
                     /translation="MAKKIRIVLTLQLPAGKATPAPPVGTALGPHGINIVEFTKTYNE
                     KTADKAGQIIPAQITIFEDRSFTFILKTPPAADLLRKAAGVDKGSGTAKRETIGRVTK
                     DQVRDIAAIKMADLSANDIEAAARMIEGTARSMGIEVGS"
     gene            1743..2447
                     /gene="rplA"
                     /locus_tag="VIF63_05800"
     CDS             1743..2447
                     /gene="rplA"
                     /locus_tag="VIF63_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY8238930.1"
                     /translation="MAHGRKYVEAAKLVEHGKIYAPDEAARLAKETTTVKFDATVEAH
                     LRLGVDPRHADQMVRGTVVLPNGTGKIVRVAVFAQGEKAQEALKAGADEVGSEDLVKK
                     IEAGWLDFDVALATPDLMGQVGRLGKILGRRGLMPNPKAGTITFDLDRAIREVKGGRV
                     EFKVDKTAIVHCPVGKASFEAEALVANLAALVDAINRAKPAGAKGTYLRTLTIASTMG
                     PGIRVDIPGILAAAQA"
     gene            2801..3325
                     /gene="rplJ"
                     /locus_tag="VIF63_05805"
     CDS             2801..3325
                     /gene="rplJ"
                     /locus_tag="VIF63_05805"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY8238931.1"
                     /translation="MPTEAKREAVAELREQLAKSRTLIVSEYRGLTVREIGEIRRSLR
                     KQDVTYRVVKNRLMKIAASDAARTALDGLLIGPTAIAFGQDEGATAKAVLDTMRPYKT
                     VRITGALLGDRAIDADGVTRLAALPSRDVLLAQLAGAFAAPMATTAGLFDAPLRDIVG
                     LVAALETQRTAAAA"
     gene            3406..>3710
                     /gene="rplL"
                     /locus_tag="VIF63_05810"
     CDS             3406..>3710
                     /gene="rplL"
                     /locus_tag="VIF63_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY8238932.1"
                     /translation="MTADQLLEAFEKMTILELSEFKKKFEDKFGVTAAVASVAAAPAA
                     GGGGAAEAAPAAEEQTEFTATITEIGPNKIPVIKVVRELTGLGLKEAKDLVDATPKAV
                     "
BASE COUNT          729 a         1306 c         1202 g          473 t
ORIGIN      
        1 atgcccggcg acaacgtcga gatgggcgtc gagctgatca ccccgatcgc gctcgaagtc
       61 ggccagcgct tcgccatccg cgagggtggc cgcaccgtcg gcgccggcgc catcaccagc
      121 atcagcgcgt aacgagatgg ccagagtcga agcgcggccg acgctccagc tcgcctgcac
      181 cgagtgcaag gagcggacgt acaccacgcg caagaacaag aagaacgatc cgaaccggat
      241 cgagctcaac aaatactgcc cacggtgtcg aaagcacacc cttcatcggg aagccaagta
      301 gcatcagcgg caccgcaggg gtgtagctca attggcagag cactggtctc caaaaccagc
      361 ggttgtaggt tcgagtccta tcgcccctgc cacaccttga aaggcaagag aacgatcacg
      421 tgaagcgaat tcgccgtttc atcgcagaag cggtctccga gctgcggaag gtctcatggc
      481 cgaccttcgt ccagacgcgc aacctgaccg tgctggtctt cgcggtcagc atcgcggtgg
      541 gcgtgttcgt cgcgatcgcc gaccagctct tccaggccgt gatcggcacg gtcgccgttt
      601 tcctgggcgg gagctgagat gagcacgacg gtcaccaccg acatcgaggc gaagcgcccc
      661 gacaagcgct ggtacgtgat ccacacgtac tcgggctacg agaacaaggt caaggcgaac
      721 ctcgagcacc gcatcgagtc gatgggcatg gaggacaaga tcttccaggt gctcgtcccg
      781 atggaggacg agatcgagat ccgccagggc cagcgtcaca ccgtccagaa gaaggtctac
      841 ccgggctacg tcctggtcga gatgatcctc gaccccgaca gctggttcgt cgtgcgcaac
      901 acgccgggcg tcaccagttt tgtcggcggc tcgaacatcc cgggcgtccg cagcgagccc
      961 gtcccgctcg acgagaccga ggtcaagcag atcctgcggc acatgggcgt cgagacgccg
     1021 aagtaccgcg tggccttcac caagggccag agcgtccggg tcaccgacgg gccgttctcc
     1081 gagttcatcg ggacggtcga cgaggtcaac cccgagcgca acaaggtcaa ggtgctcgtc
     1141 agcatcttcg gccgcgaaac acctgtggaa ctcgatttcc tccaggtaga gaagctctag
     1201 gaggagtcga acacccccat ggccaagaag atccgcatcg tgctcaccct gcagctgccc
     1261 gccggcaagg cgacccccgc cccgccggtc ggtaccgcgc tcggcccgca cggcatcaac
     1321 atcgtcgagt tcaccaagac gtacaacgag aagaccgccg acaaggccgg tcagatcatc
     1381 cccgcccaga tcacgatctt cgaggatcgc tcgttcacgt tcatcctcaa gaccccgccc
     1441 gccgcggacc tgctgcgcaa ggcagccggc gtcgacaagg gctccgggac ggccaagcgc
     1501 gagaccatcg gccgcgtgac gaaggaccag gtgcgtgaca tcgccgcgat caagatggcc
     1561 gacctctcgg ccaacgacat cgaggccgca gcacgcatga tcgaaggcac ggcccgctcg
     1621 atgggcatcg aggtcggaag ctagcaacca cgcgccggcg cttgccggct cgcctgcgcg
     1681 ggtcaccccg acgcgcaggt ccacggtggg ggaggccggt gatcccggcc gaaaggagcg
     1741 acatggcaca cggcaggaag tacgtcgagg cagccaagct cgtcgagcac ggcaagatct
     1801 acgcccccga cgaggcagcc cgactcgcca aggagacgac gaccgtcaag ttcgacgcca
     1861 cggtcgaggc ccaccttcgc ctcggcgtcg acccccgcca cgccgaccag atggtccgcg
     1921 gcacggtcgt gctcccgaac ggcaccggca agatcgtccg cgtcgccgtg ttcgcccagg
     1981 gcgagaaggc ccaggaggcg ctcaaggccg gcgccgacga ggtcggctcc gaggacctcg
     2041 tcaagaagat cgaggcaggc tggctcgact tcgatgtcgc gctcgcgacg ccggacctga
     2101 tgggccaggt cggccgcctc ggcaagatcc tcggccggcg cggcctcatg ccgaacccca
     2161 aggccggcac gatcaccttc gacctcgacc gcgcgatccg cgaggtcaag ggcggccgcg
     2221 tcgagttcaa ggtcgacaag accgcgatcg tgcactgccc ggtcggcaag gccagcttcg
     2281 aggccgaggc gctcgtcgcc aacctcgctg ccctggtcga cgcgatcaac cgggcgaagc
     2341 ccgcgggcgc gaagggcacc tacctccgaa cgctgaccat cgccagcacg atggggcccg
     2401 gcatccgagt ggatattcca gggatcctgg ccgcggcgca agcatgagcc gcgcccatcc
     2461 ggccgcctga cggggcggac caaatcgggc atactgcgca acccgccagc aatggcggtg
     2521 caaaaaacaa tcgacggaca ccagctgaag acagcctgcg cccgcggacc gcacccagcg
     2581 tgacggtcgg ggcttgatcc gccggaacgg atcggggcca acgccccggg gacgccggcg
     2641 agcatgccga ggcaggaaac ggatcgccct cacgggtggg cccgccccct gcagtccagg
     2701 tcgcgccttc gggcgcccgt cgcagaggga cggccgcggt cgaaagaccc aagcgcccac
     2761 cgccgcgcgc cggctgcgcg cctgaacgat ggagggcacc atgccgaccg aggccaagcg
     2821 cgaggccgtc gccgagctgc gcgagcagct ggcgaagagc cgcacgctca tcgtctcgga
     2881 gtaccggggt ctgaccgtcc gcgagatcgg cgagattcgc cgcagcctgc gcaagcagga
     2941 cgtgacctac cgcgtcgtca agaaccggct gatgaagatc gccgcctcgg acgccgcgcg
     3001 aaccgctctc gatgggctgc tcatcggacc gaccgcaatc gccttcggcc aggacgaagg
     3061 cgcaaccgcg aaggccgtcc tcgacacgat gcggccgtac aagaccgtca ggatcacggg
     3121 cgcgctgctc ggcgatcggg cgatcgacgc cgacggcgtc acgcgtctcg ccgcgctccc
     3181 gtcccgcgac gtcctgctgg cgcagctcgc cggtgcgttc gccgcaccga tggcgacgac
     3241 cgccggcctg ttcgatgcgc cgctgcgcga catcgtcggg ctggtcgcgg cgctggagac
     3301 ccagcgcacg gccgccgcgg cctgaccgcg acccctacga actcgacccc atcccgaacc
     3361 gaaaccaacc aaccgacgaa caggagacca gacccaatgg caacgatgac cgccgaccag
     3421 ctgctcgagg ccttcgagaa gatgaccatc ctcgagctgt cggagttcaa gaagaagttc
     3481 gaggacaagt tcggcgtcac cgccgcagtc gcgtcggttg ccgccgcgcc cgcagccggc
     3541 ggtggcggcg ctgccgaggc cgccccggcc gccgaggagc agaccgagtt caccgcgacg
     3601 atcaccgaga tcggcccgaa caagatcccc gtgatcaagg tcgtgcgcga gctgaccggt
     3661 ctcggtctca aggaggccaa ggacctggtc gacgcgacgc cgaaggccgt
//
LOCUS       DATBSW010000209         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1253756, whole genome shotgun sequence.
ACCESSION   DATBSW010000209 DATBSW010000000
VERSION     DATBSW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253756"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VIF63_05815"
     CDS             <1..1009
                     /locus_tag="VIF63_05815"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8238933.1"
                     /translation="QLELALWLEADRMGTLLDAVSQENLDNQREVVKNEKRWSYDNRP
                     YGSWVHKLLASIFPEGHPYHHPVIGSMEDLDAASLEDVSAFFRLHYAPNNAVVSVVGD
                     VDPDQVRAWVDRYFGAIPANPNLPVALPDMTLPPTLGGEIREIVPDRVPLPRIHWGFR
                     GPLYGDPRLDALDLAAQILAGGKGSRLHRRLVREELLAQDVALFSMGLVAGASVTAGW
                     ATARPGVDLVTLETAFWEELERMGREPVSADELERAKALTEADELGALQGVAEKADRL
                     SMYATLFDDPGLLNKILPRYLSTTAEQIQAVCRDVFVADNRVVLTYLPSAGADAMTSA
                     E"
     gene            1006..>1626
                     /locus_tag="VIF63_05820"
     CDS             1006..>1626
                     /locus_tag="VIF63_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY8238934.1"
                     /translation="MSDKTATVIADRPTHGEPRPFQFPRFERTELANGLGVIAVHLPG
                     RALVTSSLVLPIGAVDEPEAQAGVTSLMSRALTEGTRHHDAIGLTEAAERLGASLHAE
                     SGWDGLSISVDVPAERLGAALALVAEVASEPTFPAADVERMRDERLNDLLQAKADPRR
                     RADDEFVTTIYAPGVAYGRPSGGTEDTVRGLDAAACAALHARLLDPA"
BASE COUNT          232 a          595 c          565 g          234 t
ORIGIN      
        1 ccagctcgag ctcgcgctct ggctggaggc cgatcgcatg ggcacgctgc tcgacgcggt
       61 gagccaggag aacctcgaca accagcgcga ggtcgtcaag aacgagaagc gctggtcgta
      121 cgacaaccgg ccatacgggt cgtgggtcca caagctgctc gcttcgatct tcccggaggg
      181 ccacccgtac caccacccgg tcatcggctc gatggaggat ctcgacgcgg cctcgctcga
      241 ggacgtgtcg gcgttcttcc ggctgcacta cgccccgaac aacgccgtcg tctcggtggt
      301 gggcgacgtc gatcccgacc aggtccgggc ctgggtcgac cgctacttcg gcgccatccc
      361 ggcgaacccg aacctgccgg tcgcgctgcc ggacatgacg ctgccgccga cgctcggcgg
      421 cgagattcgc gagatcgtcc cggaccgcgt gcccctgccc cggatccact ggggctttcg
      481 tggcccgctg tacggcgacc cgcgcctcga tgcgctcgat ctggcagccc agatcctcgc
      541 cggcggcaag ggctcgcggc tgcatcgccg gctcgtccgc gaggagctgc tcgcccagga
      601 cgtcgccctg ttctcgatgg gcctcgtcgc gggcgcctcg gtgaccgcgg gctgggcgac
      661 ggcgcgaccc ggcgtcgacc tcgtcacgct cgagacggcg ttctgggagg agctcgagcg
      721 gatgggccga gagcccgttt cggccgatga gctcgaacgg gcgaaggcgc tcaccgaggc
      781 cgacgagctg ggcgcgctgc agggcgtcgc cgagaaggcc gatcgactgt cgatgtacgc
      841 gacgctcttc gacgaccccg ggctgctgaa caagatcctg ccgcgctacc tctcgaccac
      901 cgccgagcag atccaggcgg tgtgtcgcga cgtgttcgtg gccgacaacc gggtcgtgct
      961 cacgtacctc ccttcggccg gcgccgacgc catgacgagc gccgagtgag cgacaagacg
     1021 gcgacggtga tcgccgaccg tccgacccat ggcgagccgc gcccgttcca attcccgcgg
     1081 ttcgagcgaa ccgagctcgc gaacggcctc ggcgtgatcg cggtccacct gcccggccgg
     1141 gcgctggtga cctcctcgct ggtgctgccg atcggggcgg tcgatgagcc cgaggcgcaa
     1201 gccggcgtca cctcgctcat gtcccgcgca ctgaccgagg gcacgcgtca ccacgatgcg
     1261 atcggattga cggaggcagc ggaacgcctc ggcgcatcgc tccatgccga gtccggctgg
     1321 gacggcctgt cgatcagcgt cgacgtgccc gcggagcggc tcggcgcagc gctggccctc
     1381 gtcgccgagg tggcgagcga gcccacgttt ccggccgccg acgtcgagcg catgcgcgac
     1441 gagcgcctca acgacctcct gcaggccaag gccgacccac gccgtcgcgc cgacgacgag
     1501 ttcgtcacca cgatctacgc accgggcgtg gcgtacggcc ggccgtccgg cggcacggag
     1561 gacactgttc gcgggctcga tgccgccgcg tgcgctgcgc tgcacgcgcg cctgctcgat
     1621 ccggcg
//
LOCUS       DATBSW010000210         6697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_724929, whole genome shotgun sequence.
ACCESSION   DATBSW010000210 DATBSW010000000
VERSION     DATBSW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724929"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VIF63_05825"
     CDS             complement(<1..584)
                     /locus_tag="VIF63_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238935.1"
                     /translation="MTEAEMTEQKTAAIETDEPTEADATVENDAPMAAAESTETAVSA
                     ETAEEPVAAEASATAADEAPVAAEEPTTASDEPVAAVDESTAAPDEPVAAGDESTAAA
                     DEPAADAPKASKSKAPAKAPKAAADERKAAATAAPAAAPEAPAPARAAKPVLEGPRPL
                     RIRYKTEVVPALVKQFEYANPNQVPKLEKIVVNIG"
     gene            complement(581..1039)
                     /gene="rplX"
                     /locus_tag="VIF63_05830"
     CDS             complement(581..1039)
                     /gene="rplX"
                     /locus_tag="VIF63_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY8238936.1"
                     /translation="MALRKTRVPEIRKGDTVLVLSGKDAGKQGLVERVLTNPAALQRV
                     TGSGSDKGRKLRTGFWKPASSRPVSVVVEGLNIAKRHTKPTRTQGRNDRAPKVQSGGI
                     LDIAQPLDVSKVMLVCPSCKEPTRVRHTVLEDGRRVRVCTHCGKAIEVTA"
     gene            complement(1039..1407)
                     /gene="rplN"
                     /locus_tag="VIF63_05835"
     CDS             complement(1039..1407)
                     /gene="rplN"
                     /locus_tag="VIF63_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY8238937.1"
                     /translation="MIQAQTRLKVADNTGARTIQCIRVMGRSTKTTATVGDVIVASVK
                     TAIPNAAVKKGDVVQAVIVRTAKEYGRPDGSYIRFDENAAVLINNQGNPRGTRIFGPV
                     ARELRDRNYMKIVSLAPEVL"
     gene            complement(1569..1835)
                     /gene="rpsQ"
                     /locus_tag="VIF63_05840"
                     /pseudo
     CDS             complement(1569..1835)
                     /gene="rpsQ"
                     /locus_tag="VIF63_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            complement(1842..2099)
                     /gene="rpmC"
                     /locus_tag="VIF63_05845"
     CDS             complement(1842..2099)
                     /gene="rpmC"
                     /locus_tag="VIF63_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY8238938.1"
                     /translation="MTDIAKVREMSDKELLDALQQAKQDLWSARFALSTRQLNDTSGI
                     PKTRRTIARILTVQRERGTGRGAAASVAKATAVPAKTGKGA"
     gene            complement(2096..2527)
                     /gene="rplP"
                     /locus_tag="VIF63_05850"
     CDS             complement(2096..2527)
                     /gene="rplP"
                     /locus_tag="VIF63_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY8238939.1"
                     /translation="MLLPRRVKHRKVMRGRMSGVAKGGHYVAFGDYGLATIEPAWITS
                     RQIEAARRAMTRSVKRGGKIWIRVFPDKPVTKKPAETRMGSGKGAPDHWVAVVKPGRI
                     LFEMAGVPHDEAAEAMRLASHKLPVQTRVVMREGAAEGGER"
     gene            complement(2531..3208)
                     /gene="rpsC"
                     /locus_tag="VIF63_05855"
     CDS             complement(2531..3208)
                     /gene="rpsC"
                     /locus_tag="VIF63_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY8238940.1"
                     /translation="MGHKVHPYGFRLGVTRTWTAKWYHDKEYTALLTEDIFIRKLVEK
                     RLANASVSGLEIERNFGQVTVTVHTAKPGIVIGKGGQNVEILRQQIGALTKRKVKLEI
                     KEIRQPELDAYLVATNISQQLSRRIAFKKAMKQSIQRSMKAGAKGVKIMVAGRLGGAE
                     MSRREWDREGRIPLGTLRADISYGQVHAHTTYGRIGVKVWIYRGDVTPTRTAARDAVP
                     ATAVQGA"
     gene            complement(3214..3546)
                     /gene="rplV"
                     /locus_tag="VIF63_05860"
     CDS             complement(3214..3546)
                     /gene="rplV"
                     /locus_tag="VIF63_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY8238941.1"
                     /translation="MSATAKYLRGSTRKARLVVAVIKGRSVEEAAALLRFMPQHAAKD
                     VAGVLKSAAANAENNHNLAADELTVLDARADQGPTIKRFRPRAQGRAFPIHKGVTHIT
                     VTVGDREG"
     gene            complement(3560..3844)
                     /gene="rpsS"
                     /locus_tag="VIF63_05865"
     CDS             complement(3560..3844)
                     /gene="rpsS"
                     /locus_tag="VIF63_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY8238942.1"
                     /translation="MSRSIKKGPFIEPRLLGRIELMNKKSEKKVVKTWSRASVIFPSF
                     IGHTVAVYNGRKHVPVYVTENMVGHRLGEFAPTRTYRGHGKSTDRATAPK"
     gene            complement(3860..4684)
                     /gene="rplB"
                     /locus_tag="VIF63_05870"
     CDS             complement(3860..4684)
                     /gene="rplB"
                     /locus_tag="VIF63_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8238943.1"
                     /translation="MPLRSYKPTSPGMRGMTRSTFDEITTTEPYKPLLEPQIRGSGRN
                     NQGRLTVRHRGGGEKTHYRRIDFKRDKHDVPARIATVEYDPNRSARIGLLHYRDGEKR
                     YMLLPHGLKVGDVVVAGSGADARVGNALPIANIPLGTTIHNIELIPGKGGQIVRSAGT
                     AAQLLAKEGEYAQVRLPSGEVRRISIRCMATVGQVGNLDHENQNLGKAGRARHMGQRP
                     EVRGVVMNPRDHPHGGGEGKSPTGMPPKTPWGKPAMGYRTRRARTGGLIVRSRHRK"
     gene            complement(4686..5024)
                     /gene="rplW"
                     /locus_tag="VIF63_05875"
     CDS             complement(4686..5024)
                     /gene="rplW"
                     /locus_tag="VIF63_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8238944.1"
                     /translation="MSALTAPEIIVRPVVSEKSIDQSTLTDRNPKIGNKYTFRVHPDA
                     NKIQIKAAIEELYAGDKVSVVSVNVLTTKSKQKSRGTKRGRIKGSTSAWRKAVVTLAA
                     GQKIQFFEGV"
     gene            complement(5021..5644)
                     /gene="rplD"
                     /locus_tag="VIF63_05880"
     CDS             complement(5021..5644)
                     /gene="rplD"
                     /locus_tag="VIF63_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8238945.1"
                     /translation="MPKTILYDRTGANVGSVELHDDLFAAPVNEAVLHQVVVAQLAGR
                     RIGTADTKTRGEIRGGGKKPYRQKGTGRARQGTPTAPHYRGGGVVFGPHPRSYEQRLP
                     RKMKRLALRSALTAKLGDEAIRILDTFGLEAIKTQDVVGVLLALKATGRVLVVAPNAD
                     EKLRLSARNLPTVEVILADSLNVVDVLKADTLVIEQLALARMEEVYL"
     gene            complement(5648..6256)
                     /gene="rplC"
                     /locus_tag="VIF63_05885"
     CDS             complement(5648..6256)
                     /gene="rplC"
                     /locus_tag="VIF63_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY8238946.1"
                     /translation="MIGLIGRKVGMTQVFQKDGTMVAVSVLKVAPNTVTNLRTPDRDG
                     YTAIQLGTDEKKKLTKPEAGQLGDLPALGTIREFRVDEIGEYEVGQTIAVGDMFAEGD
                     LVDVSGVSKGKGFAGHIKRHNFHRGPTTHGSDHHREPGSIGPGTTPGRVYKGVKMAGH
                     MGDAQVTTKKVRVIRADAENNLLLVKGSLPGGKGSLIMVRKV"
     gene            complement(6253..6561)
                     /gene="rpsJ"
                     /locus_tag="VIF63_05890"
     CDS             complement(6253..6561)
                     /gene="rpsJ"
                     /locus_tag="VIF63_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY8238947.1"
                     /translation="MAKQRIRIRLKAYDHRLLDQSAAQIVETAQRTGADIVGPVPLPT
                     RIEKFTVLRSPFIDKDSREQFEIRTHKRLVDILDPSSKTVDALMRLSLPAGVDIEIKM
                     "
     gene            complement(6566..>6697)
                     /gene="tuf"
                     /locus_tag="VIF63_05895"
     CDS             complement(6566..>6697)
                     /gene="tuf"
                     /locus_tag="VIF63_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY8238948.1"
                     /translation="MPGDNVEMGVELITPIALEVGQRFAIREGGRTVGAGAITSISA"
BASE COUNT          817 a         2243 c         2366 g         1271 t
ORIGIN      
        1 ccgatgttga ccacgatctt ctcgagcttc ggcacctggt tcgggttggc gtactcgaac
       61 tgcttgacga gcgcgggcac gacctcggtc ttgtaccgga tgcgcagcgg gcgcggccct
      121 tcgaggaccg gcttcgcggc ccgcgccgga gcgggggctt cgggcgcggc cgccggcgcg
      181 gcggtcgcgg cagccttgcg ctcgtcggcc gcggccttcg gggctttcgc aggcgccttc
      241 gacttcgacg ccttcggcgc gtcggcggcc ggctcgtcgg cagctgcggt cgactcgtcg
      301 cccgctgcga ccggttcgtc gggggcagcg gtcgactcgt ccacggcagc gaccggctcg
      361 tcggaagcag ttgtcggctc ttcggcggca accggcgcct cgtcggcagc agtcgcggac
      421 gcctcggcag ccacgggctc ctcggcggtc tcggcgctca ccgcggtctc ggtcgattcg
      481 gctgcggcca tcggcgcgtc gttctccacc gtcgcgtcgg cctcggtcgg ctcgtcggtc
      541 tcgatcgcgg cggtcttctg ctcggtcatc tcggcctcgg tcatgccgtc acctcgatgg
      601 ccttgccgca gtgcgtgcag acgcgcacgc gccggccatc ctcgaggacg gtgtggcgca
      661 cgcgggtcgg ctccttgcag ctcgggcaga ccagcatcac cttcgacaca tcgagcggct
      721 gcgcgatgtc gaggatgccg cccgactgca ccttcggggc gcgatcgttg cgaccctggg
      781 tgcgtgtcgg cttggtgtgg cgcttggcga tgttcaagcc ctcgaccacg accgacaccg
      841 gccgtgacga cgcgggcttc cagaaccccg tgcggagctt ccggcccttg tccgatccgc
      901 tcccggtcac gcgctgcagc gcggcggggt tggtcaggac gcgctcgacg aggccctgct
      961 tgccggcatc cttgccggac aggaccagga cggtgtcgcc cttgcggatc tcgggcacgc
     1021 gcgtcttgcg aagggccatc acagcacctc cggggcgagc gagacgatct tcatgtagtt
     1081 gcggtcacgg agctcgcgcg cgaccggccc gaagatgcgg gtcccgcgcg ggttgccctg
     1141 gttgttgatc agcacggcgg cgttctcatc gaaacggatg tagctcccat cggggcggcc
     1201 gtactccttg gccgtccgca cgatgacggc ctggaccacg tcgcccttct tgacggcggc
     1261 gttcgggatc gcggtcttga cgctcgcgac gatcacgtcg ccaaccgtgg cggtggtctt
     1321 cgtcgagcgg cccatcacgc ggatgcactg gatggtccgg gcacccgtgt tgtcggcgac
     1381 cttgaggcgg gtctgggcct ggatcacgcg tcgtcaccct tctcgggcgt ctcggccgtc
     1441 tcggcggtct ccgtctcggt gtccgcatcg tcatcgtgct tggtgcgacc cggatgggcg
     1501 gccgagtgga tcgcctcgga gacctcggcc tcctcggcga cggtgtcgag cggcgcgccg
     1561 tgatcgccgg cgcgctggac gacctcgagc aggcgccacc gcttcgtcgc cgacagcggg
     1621 cgcgattcct cgatcaggac cgtatcgccg acgtgggcgt cgttggtctc gtcgtgcgcc
     1681 ttgaacttcg tggtcaggcg gatgacccgc ttgtacagcc ggtggcgggt gagccgctcg
     1741 acggacacga cgatcgtctt gtccatcttg tcgctcacca cgcgcccgac cttggtcttg
     1801 cgcttgcctt gcacggcggc aggtccttcg gtcatggcca tctatgcgcc ctttccagtt
     1861 ttcgccggta cggcggtcgc cttcgcgacg ctcgccgccg cgccgcgacc ggtcccgcgc
     1921 tcgcgctgga cggtgaggat gcgggcgatg gttcgacggg tcttcggaat cccgctggtg
     1981 tcgttgagct gacgggtcga gagcgcgaat cgggcgctcc acaggtcctg cttcgcctgc
     2041 tggagcgcgt cgagcagctc cttgtcggac atctcgcgga ccttggcgat gtcggtcatc
     2101 gctcgcctcc ctcggcagcg ccctcgcgca tcacgacgcg ggtctggacg ggcagcttgt
     2161 ggctcgccag gcgcatcgcc tcggcggctt cgtcatgcgg cacgccggcc atctcgaaca
     2221 ggatccggcc cggcttcacg accgcgaccc agtggtccgg cgcgcctttg cccgagccca
     2281 tgcgcgtctc ggccggcttc ttggtaaccg gcttgtcggg gaagacccgg atccagatct
     2341 tgccgccgcg cttgaccgag cgggtcatcg cgcgtcgggc ggcctcgatc tgccggctcg
     2401 tgatccacgc cggctcgatc gtcgccagtc cgtagtcgcc gaatgcgacg tagtggccac
     2461 ccttggccac gccggacatg cgaccgcgca tgaccttgcg atgcttgacg cgtcgcggca
     2521 ggagcattgc tcaggctccc tggacggctg tggcggggac ggcgtcacga gcggcggttc
     2581 gcgtgggcgt gacgtcgccg cggtagatcc agaccttcac gccgatgcga ccgtaggtcg
     2641 tgtgggcgtg cacctggccg tagctgatgt cggcgcgcag cgtgccgagc ggaatgcgac
     2701 cttcacggtc ccattcgcgg cggctcatct cggcgccacc gaggcggccc gcgaccatga
     2761 tcttgacgcc cttggcgccg gccttcatcg agcgctggat cgactgcttc atcgccttct
     2821 tgaaggcaat tcggcggctc agctgctggc tgatgttggt cgcgacgagg tacgcgtcga
     2881 gctccggctg gcggatctcc ttgatctcca acttgacctt gcgcttcgtg agcgcgccga
     2941 tctgctggcg caggatctcg acgttctggc cgcccttgcc gatcacgatg cccggcttgg
     3001 cggtgtgcac ggtgacggtc acctggccga agttgcgctc gatctcgagg ccgctgacgc
     3061 tcgcgttggc gagccgcttc tcgacgagct tgcggatgaa gatgtcctcg gtcaggagtg
     3121 ccgtgtactc cttgtcgtgg taccacttcg cggtccaggt gcgcgtcacc ccgagtcgga
     3181 acccgtaagg gtgaaccttg tgtcccatgt tcctcagccc tctcggtcgc cgacggtgac
     3241 ggtgatgtgg gtgacgccct tgtggatcgg gaacgcccgg ccctgtgcgc gcgggcggaa
     3301 gcgcttgatc gtcgggccct gatcggcgcg cgcgtcgagc accgtgagct cgtcggcggc
     3361 caggttgtgg ttgttctcgg cgttcgcggc cgcgctcttc agcaccccgg ccacatcctt
     3421 cgcggcgtgc tggggcatga acctgagcag ggcggcggct tcttccacgg accgaccctt
     3481 gatgacggcc acgacgaggc gggccttgcg ggtcgaaccg cggaggtact tggctgtcgc
     3541 tgacactctc atcgtcggct cacttcggcg ccgtcgcgcg gtcggtgctc ttgccgtgcc
     3601 cgcgataggt gcgggtcggc gcgaactcac cgaggcggtg gccgaccatg ttctcggtca
     3661 cgtacaccgg cacgtgcttg cggccgttgt agaccgcgac cgtatggccg atgaaggacg
     3721 ggaagatcac ggacgcgcgc gaccaggtct tgacgacctt cttctcggac ttcttgttca
     3781 tgagctcgat gcggccgagc agccggggct cgatgaacgg gcccttcttg atggatcggg
     3841 acatctctcg cctcccctgc tacttccggt gccgcgagcg gacgatgagc ccacccgttc
     3901 gagcgcgcct ggtgcgatag cccatggcgg gcttgcccca tggcgtcttg ggcggcatgc
     3961 cggtcgggga cttgccctcg ccgccgccgt gtggatggtc gcgcgggttc atgacgacgc
     4021 cacggacctc gggccgctgc cccatgtgcc gggcgcggcc ggccttgccg aggttctggt
     4081 tctcgtgatc gaggttgccg acctggccga cggtggccat gcaccggatg ctgatccggc
     4141 ggacctcgcc ggacggcagc cggacctggg cgtactcgcc ttccttggca agcagctggg
     4201 cggcggtccc ggcggagcgg acgatctggc cgcccttgcc cgggatgagc tcgatgttgt
     4261 ggatcgtcgt gccgagcggg atgttcgcga tcggcagggc gttgcccacg cgcgcgtccg
     4321 cgcccgagcc ggcgacgacg acgtccccga ccttcaggcc gtggggcagg agcatgtagc
     4381 gcttctcgcc gtcgcggtag tggagcaggc cgatgcgcgc cgaccggttc gggtcgtact
     4441 cgaccgtggc gatgcgcgcg ggcacgtcgt gcttgtcacg cttgaagtcg atgcgccggt
     4501 agtgcgtctt ctcgccgccg ccgcgatggc gcacggtgag gcggccctgg ttgttccggc
     4561 cggagccgcg aatctggggc tcgagcaggg gcttgtacgg ctcggtcgtc gtgatctcgt
     4621 cgaacgtcga gcgcgtcatg ccgcgcatgc cgggcgaggt gggcttgtag gaacgcagcg
     4681 gcatctcaga cgccctcgaa gaactggatc ttctggccgg cggccagggt cacgacggcc
     4741 ttgcgccacg ccgacgtcga gcccttgatc cgtccgcgct tggtgccgcg cgacttctgc
     4801 ttggacttcg tcgtcagcac gttgaccgac acgacgctga ccttgtcgcc cgcgtagagc
     4861 tcctcgatcg ccgccttgat ctggatcttg ttggcgtcgg ggtgcacgcg gaacgtgtac
     4921 ttgttgccga tcttcgggtt gcggtcggtc agcgtcgact ggtcgatcga cttctcgctc
     4981 acgaccgggc gcacgatgat ctccggcgcc gtcagggcgc tcacaggtag acctcttcca
     5041 tgcgcgccag cgcgagctgc tcgatcacga gcgtgtcggc cttgagcacg tcgacgacgt
     5101 tgagcgagtc ggcgaggatg acctcgacgg tcggcaggtt gcgcgccgag aggcggagct
     5161 tctcgtccgc gttcggggcg acgacgagca cgcgacccgt ggccttgagc gcgagcagca
     5221 cgcccacgac gtcctgggtc ttgatcgcct cgagcccgaa ggtgtcgagg atgcgaatcg
     5281 cctcgtcgcc gagcttggcg gtcagcgcgg agcgaagggc gaggcgtttc atcttgcggg
     5341 gcaagcgctg ctcgtacgag cgggggtgcg ggccgaacac gacgccgccg ccgcggtagt
     5401 gcggcgcggt cggcgtgccc tggcgggcgc ggccggtgcc cttctggcgg tacggcttct
     5461 tgccgccgcc gcggatctcg ccgcgggtct tggtatcggc ggtgccgatc cggcggccgg
     5521 cgagctgggc gacgacgacc tggtgcagca cggcctcgtt gaccggcgcg gcgaacaggt
     5581 cgtcatgcag ctcgacgctg ccgacgttgg ctccggttcg gtcgtacagg atggtcttgg
     5641 gcatcgctca gaccttccgg accatgatca ggctgccctt gccgccgggc aggctgccct
     5701 tgacgagcag gaggttgttc tcggcgtcgg cgcggatcac gcgcaccttc ttcgtcgtga
     5761 cctgcgcgtc gcccatgtgg ccggccatct tcacgccttt gtacacgcgg cccggcgtgg
     5821 tgccggggcc gatcgagccg ggctcgcggt ggtggtcgga accgtgggtc gtgggcccgc
     5881 ggtggaagtt gtggcgcttg atgtggccgg cgaagccctt gcccttcgag acgccgctca
     5941 cgtcgacgag gtcgccctcg gcgaacatgt ccccgaccgc gatcgtctgt ccgacctcgt
     6001 actcgccgat ctcgtcgacg cggaactcac ggatcgtgcc gagcgccggc aggtcgccga
     6061 gctggccggc ttcgggcttg gtgagcttct tcttctcgtc cgtgccgagc tggatggcgg
     6121 tgtagccgtc gcgatcgggc gtgcgcaggt tggtcaccgt gttcggggcg accttgagca
     6181 ccgagacggc aaccatcgtg ccgtccttct ggaacacctg cgtcatcccg acctttcggc
     6241 cgatcagccc aatcacatct tgatctcgat gtcgacgcca gccgggaggc tgagtcgcat
     6301 cagggcgtcc acggtcttcg acgagggatc caggatgtcg acgagccgct tgtgggtgcg
     6361 gatctcgaac tgctcccggc tgtccttgtc gatgaacggt gagcgcagga cggtgaactt
     6421 ctcgattcgg gtaggcagcg gcacgggccc gacgatgtcg gcacccgtac gctgagccgt
     6481 ctcgacgatc tgcgccgccg actgatccaa cagccggtgg tcgtaggcct tgagacggat
     6541 ccggatccgc tgctttgcca tctcgttacg cgctgatgct ggtgatggcg ccggcgccga
     6601 cggtgcggcc accctcgcgg atggcgaagc gctggccgac ttcgagcgcg atcggggtga
     6661 tcagctcgac gcccatctcg acgttgtcgc cgggcat
//
LOCUS       DATBSW010000211         6921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1077251, whole genome shotgun sequence.
ACCESSION   DATBSW010000211 DATBSW010000000
VERSION     DATBSW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077251"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..2881
                     /locus_tag="VIF63_05900"
     CDS             257..2881
                     /locus_tag="VIF63_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238949.1"
                     /translation="MRLLEREDSLATLQRLRAEAVSDGGRLVFVEGEAGVGKTALLGA
                     FRRTLSGGVLCLLGSCDPLSTPRPLGPLVEIADELDPTFARLVRDGAPRTDVLGGLLD
                     ALRRHGANLVLLLDDLHWADEATLDALRFVGRRIETTSVLIVGTYRDDEVGPKHPLRV
                     VVGDLATSPAVRRLPLAGLSVGSVGELVGGTGIDPVELHELTGGNPFYVTEVIAGAPA
                     RIPATVRDAVLARAARLSPPARRALEAAAVIGPVVDPALLSTIVGDGGAEECLALGLL
                     IAQGRAYAFRHEVARRAILDAVDPIARTRLHARVLEALERDHRGAAAPAALLAHHAAE
                     ALDGGAVLRLAPEAARQAAAASAHREAAAHYAGALVYAGLLPAVERAILLEAAGREHT
                     LVARYDVGRRAYEEAIAIWQGVGDARRATAALAELSKVLVTAGRNAEAELASRQALET
                     AGRLADGPEKVEAINAQAYLRMLDRDNDEAVRMGRSAIDLGVGEPRAVASVVQAWNTV
                     GSSRILLGDDGGRADLETSLRLALEHGLERNAASAYAVLASALGEMYRFADAEPYFEA
                     GIRYASERDLDSSRLYLEAWRALTLVHRGRWSEAGTLAAQVLAREDNSAISRIMALLA
                     VGRLRARRGDPDVWLALDEALTLSEPTATLQRLGPVRAARAEAAWLDGDLTRAAAEAA
                     SVFELAREKRHPWHIGELGWWQRVAGLPAPDVGGAAEPWRLQHEGRWREAAAAWRVLD
                     CPYESARALLDADDVTAVTEAHAEFDRLGARPARALAARRLRDLGVTVVPRGRRPTTA
                     ANAAGLTTRELEVLRLVTEGLPNSDIAARLFLSPRTVDHHVSAVLGKLGVGRRSDAAA
                     AAARAGIAPQNGQSVEPD"
     gene            2966..3253
                     /locus_tag="VIF63_05905"
     CDS             2966..3253
                     /locus_tag="VIF63_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY8238950.1"
                     /translation="MPRYVVERSFPEGFELPFGQAGVNVCRGVVGINENLGVTWLHSY
                     VREDRRTTFCIYDAPNPEAIRRAAERNGLPVDRITRVSVLDPYFYTPSGDA"
     gene            3379..3627
                     /locus_tag="VIF63_05910"
     CDS             3379..3627
                     /locus_tag="VIF63_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO4226 family nickel-binding protein"
                     /protein_id="HEY8238951.1"
                     /translation="MARFMDVHDGFFGVSAEQLAEAHRRDLAIQDDEDVVYERAWLDP
                     ESGKVFCLATGPSKEAVMRIHERAGHPTPNVYEVPIEV"
     gene            3624..4163
                     /locus_tag="VIF63_05915"
     CDS             3624..4163
                     /locus_tag="VIF63_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238952.1"
                     /translation="MRTNTIRIPRRPRAAAAALLLALSLLTAFAGSMVGASKADIPAI
                     RAATLKYKDISAAIADNYVEFYQCTEQPGVGTMGQHFVNLGLVGDPAIEPLRPEVLVY
                     APKRGGGYRLVAVEYVTFAAGQTVLGQSMNPVGPGNRYGLDPFFAKHAWIWQGNPKGI
                     FADWNPSITCLGNGDNGGG"
     gene            complement(4270..>6921)
                     /locus_tag="VIF63_05920"
     CDS             complement(4270..>6921)
                     /locus_tag="VIF63_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8238953.1"
                     /translation="IQDLARSMDARDWGDVGPRLLGVDRAQGPDSLGSRRGLVRDIVE
                     MMLGTVDQPTLLAFEDLQWADEMTLEVIAELARGGRELPLLLVGVYRLDELPAASIHR
                     EWRARLLTQRLAEEARVQRLTKDQTALVTTLLLGTGLPASREVVEAVHARTNGIPLHV
                     EELLAAVGGSTTDGRSILGAHVPDTIEDAILAHATRLSDEARAVARAGAVLGRCFVPH
                     VLAGILDRPLAALDEPLEELVNAGILFPFDYVDEGYFDFRHQLLRDALYDDIPARDLR
                     RFHARAAEFGASLVGSTDASLHFERAGLHDQAFRAALDAAEAAKRVYSNREAFDLYRR
                     AVQNMPTTLPDVEKARVWMSYSDAAGAVDRNDICRDAAVQSRELALRAGDATAAAEAL
                     VNIAIVTRREGESVVEFRDLSRRLALEVERLPEGERARVLHVAALDFLAIAEVDDLHF
                     ADARALFDESRDAAVKWGLEEDAEHAATELAMLDVLEGRPEPALTILIESADRARSAG
                     HETHAVNAYRDAAWYATRSLDYRKAGRQIGEGLKYAEEIEQSFCGHLLASTQAMVSWA
                     DGRWAEATEQGGQAASDPGSARSRAIARWVLGWIAAGRGDRAEAESHLQPSIEYGRKA
                     GWLEYVLPPQWGLAEAALMAGDPQAAIEHCEAALRDARQRGEWGLLAPFVVTGVRAYQ
                     TAGKPEAAAKWLEQVKRAIGPLAEVAAPAIQHGTGLVKLGEGSIVAAREALEGAIHAW
                     DGRGRRWEGLWARLDLATADLRANRYAEAITLVREVTDAAETMGSRPLLDRASHLTRL
                     AKGRGAEVDPWHPLTIREFEVARKIAEGLTNAQIAGELFVSPKTVSAHVEHILAKLGA
                     GRRAEIAAWVSSVPAASVAVSDGQRVA"
BASE COUNT          979 a         2459 c         2445 g         1038 t
ORIGIN      
        1 catgatcagg gtgagtgtcg gcagggtctc gacgccgagc ttgatgaagg cgtagctcgt
       61 gccccacatc agcccgagga cgaagagcag gacccagtcg gtgcgcgtcg ggcgggcgat
      121 catcggtgga gtgtagcgtg actttggcct agacgagtgt gccaacgaag gctcttgagg
      181 cggtccgggg gcacggcaac ggtcacgaat tgcacaacac gacggcgggc aggatcgttg
      241 cgactacgat gccgccatgc gcctcctcga gcgcgaagat tctctcgcga cgctacagcg
      301 gctgcgcgct gaagcggtat ccgacggggg tcggctggtg ttcgtcgagg gcgaggcagg
      361 cgtcggcaag accgcgctcc tcggggcgtt tcgcaggacg ctgtcgggcg gtgtgctgtg
      421 cctgctggga tcctgcgacc cactctcgac cccgaggccg cttggacccc tggtcgagat
      481 cgccgacgag ctcgacccga cgttcgcccg gctcgtccga gacggtgcac cgcgcaccga
      541 cgtcctgggc gggctgctcg acgcgctccg ccgccatggc gcgaatcttg tgctcctcct
      601 cgacgacctc cattgggcgg acgaggcgac gctcgacgcg ctccgtttcg tcgggcgtcg
      661 gatcgagacc accagtgtcc tgatcgtggg cacgtaccgc gacgacgagg tcgggccgaa
      721 gcacccgctg cgcgtcgtcg tcggcgacct tgcgacctcg cctgcggttc gccgcctgcc
      781 gttggccggc ctgtccgtgg ggtcagtcgg cgagctcgtt ggcgggacgg gcatcgaccc
      841 cgtcgagctc cacgagctga ccggcgggaa cccgttctac gtgaccgagg tcatcgccgg
      901 cgcaccggcg cggatcccgg cgacagttcg agatgccgtg ctggcccgcg ccgcccgcct
      961 ttcgccgccg gcgcggcgtg ccctcgaagc ggcggccgtg atcgggccgg tcgtcgatcc
     1021 ggcgctgctc tcgaccatcg tgggcgacgg cggggcagag gagtgcctcg cgctcggcct
     1081 gctcattgcg cagggccggg cgtatgcctt ccggcacgag gtggcgcgac gggcgatcct
     1141 cgatgcagtc gatccgatcg cccgcacccg actgcatgcc cgagtcctcg aggcgctcga
     1201 gcgcgaccac cgcggagcgg cagcgcctgc tgccctgctc gcgcaccacg ctgcggaggc
     1261 gctcgacggc ggcgccgtgc tgcggctcgc gcccgaggcc gcacggcaag ccgctgctgc
     1321 gtccgcgcat cgcgaggccg cagcccacta cgcgggtgcc ctggtgtacg ccgggctgtt
     1381 gccggcagtc gagcgcgcga tcctgctcga ggccgccggt cgcgaacaca cgctggtcgc
     1441 caggtacgac gtcggacgtc gtgcgtacga agaggcgatc gccatctggc agggcgtcgg
     1501 cgatgcccgc cgtgcgaccg ctgccctcgc cgagctctcg aaggtccttg tgacggccgg
     1561 ccgcaacgcc gaggccgaac tggcttcccg ccaggcgctc gagacggcgg gacgtctcgc
     1621 ggacggcccc gagaaggtcg aggcgatcaa tgcccaggcc tacctccgga tgctcgatcg
     1681 ggacaacgac gaggccgtgc ggatggggcg ctccgcgatc gacctcggcg tcggcgaacc
     1741 gcgggcggtc gcgtcggtcg tccaggcgtg gaacacggtg ggcagctcgc ggatcctgct
     1801 cggcgacgat ggcggcaggg ccgacctcga gacgagcctg cgcctcgccc tcgagcacgg
     1861 gctggagcgg aatgccgcgt cggcgtacgc ggtcctcgcg tcggcgctcg gcgagatgta
     1921 ccggttcgcg gatgcggagc cgtacttcga ggccggcatc cgctacgcgt cggaacgtga
     1981 cctcgattcg tcgcgcctgt acctcgaggc gtggcgcgcg ctgacgctcg tgcatcgcgg
     2041 acgctggtcg gaggcgggca ccctcgcggc gcaggtgctg gcgcgcgagg acaactccgc
     2101 gatcagccgg atcatggccc tgcttgccgt gggccggctg cgcgctcggc gcggcgaccc
     2161 ggatgtgtgg ctcgccctcg acgaggccct gaccctgtcc gagcccacgg ccacgttgca
     2221 gcgtcttggg ccggtcaggg ccgcgcgcgc cgaggcagca tggctcgatg gcgacctcac
     2281 ccgcgcggcc gccgaggcgg cgtcggtctt cgagctggcg cgcgagaaac ggcacccgtg
     2341 gcacatcggt gagctcgggt ggtggcaaag ggtggcgggc ctccctgctc cggatgtcgg
     2401 cggcgctgcc gagccgtggc ggctccagca cgaaggtcga tggcgcgagg ccgccgccgc
     2461 gtggcgcgtc ctggactgcc cctacgagtc cgcccgagcg ctgctcgatg ccgacgacgt
     2521 gacggccgtc accgaagctc acgccgagtt cgaccggctc ggcgctcggc cggccagggc
     2581 gttggccgcc cggcgcctgc gcgacctcgg cgtcacggtc gtgccccggg ggcgccgccc
     2641 gacgacggcc gcgaacgcgg cgggcctgac gacccgcgag ctcgaagtgc tcaggctcgt
     2701 gacggagggc ctgccgaaca gcgacatcgc ggcgcgcctg ttcctgtcgc cgcgaaccgt
     2761 cgatcaccac gtgtcggcgg tgctcggcaa gctcggcgtc ggtcgccgga gcgacgccgc
     2821 ggcggccgcc gccagagccg ggatcgcgcc ccagaacggg cagtcggtgg agccagatta
     2881 ggcagccacc accgatgacc tgacggcgcc gcaccgcgaa cctcgtgctg cggccgacgt
     2941 ccggccgccg tcgatggagg tgctggtgcc gcgatacgtg gtggagcggt cattcccgga
     3001 gggcttcgag ctcccgttcg gccaggccgg cgtgaatgtc tgccgcggcg tcgtggggat
     3061 caacgagaac ctcggcgtga cgtggcttca ctcgtacgta cgcgaggacc ggcggacgac
     3121 gttctgcatc tacgacgcgc cgaatcccga ggcgatccgg cgcgccgccg aacggaacgg
     3181 cctgcccgtc gaccgaatca cccgcgtcag cgtcctcgac ccgtacttct acacgccgtc
     3241 gggcgacgcc tgacgggatc gaaatcggtc gctcggtggg gggctcgtcg gtcggtcgac
     3301 cgatgaccca ggccggtcca gcgaccagcc tgcaacgaga aggcgcggca tgaccgcacc
     3361 gcaccaagga ggtactcgat ggccaggttc atggatgtcc acgacgggtt cttcggcgtg
     3421 tcggccgaac agctcgccga ggcgcatcgg cgcgatctcg cgatccagga cgacgaggac
     3481 gtcgtctacg agcgcgcgtg gctcgacccc gagagcggca aggtcttctg cctcgccacc
     3541 gggccgtcga aggaggccgt gatgcgcatc cacgagcgtg cggggcatcc gaccccgaac
     3601 gtctacgaag tcccgatcga ggtttgagga cgaacacgat ccgaatcccg cgccgccccc
     3661 gggcggccgc cgccgcgctc ctcctggcgc tctcgctcct caccgcgttc gccggctcga
     3721 tggtcggcgc cagcaaggcc gacatcccgg ccatacgtgc cgcgacgctc aagtacaagg
     3781 acatcagcgc ggccatcgcc gacaactacg tcgagttcta tcagtgcacc gagcagcccg
     3841 gtgtcggcac catgggccag cacttcgtga atctcgggct ggtcggtgac ccggcgatcg
     3901 agccgctccg acccgaggtg ctcgtctacg cgccgaagcg cggcggcggg taccgcctcg
     3961 tcgctgtcga gtacgtcacg ttcgcggccg gccagaccgt cctcggacag tcgatgaacc
     4021 ccgttggacc gggcaaccgg tatggcctcg acccgttctt cgcgaagcac gcgtggatct
     4081 ggcagggcaa tcccaagggc atctttgccg actggaaccc gtcgatcacg tgcctgggca
     4141 acggcgacaa cggcggcggc tgagcaatcg atcgagcgag cctcctccgg gcgctccctg
     4201 gggaagtttc gaggccggtc ccggcgacgg gaccggcctt ctcgttgtgt ctgagcgcgt
     4261 tgcctggcct tatgcgacgc gctgcccgtc ggagacagcg acagatgcgg ccggcaccga
     4321 cgaaacccac gccgcgatct cggcgcggcg gcctgccccg agcttggcga ggatgtgctc
     4381 gacgtgggcg ctcacggtct tcggcgagac gaagagctcc cctgcgatct gcgcattcgt
     4441 cagcccttcg gcgatcttcc gcgcgacctc gaactcccgg atcgtcaatg ggtgccacgg
     4501 gtcgacctcg gcgccccggc ccttggcgag tcgagtcagg tggctcgctc ggtcgagcag
     4561 gggtcgactg cccatcgttt cggcggcatc cgtgacctcg cgcacaagcg ttatcgcttc
     4621 ggcatagcgg ttcgcgcgca ggtctgccgt cgcgaggtcc aggcgggccc acaggccctc
     4681 ccaccggcgg ccgcgaccgt cccatgcatg gatcgcaccc tccagcgcct cacgtgccgc
     4741 cacgatcgac ccctccccga gcttgacgag accggttcca tgctggatcg cgggtgcggc
     4801 cacctcggcc agtggcccga ttgcccgttt cacctgttcc agccatttgg ccgccgcttc
     4861 cggcttgcct gccgtctggt acgcccgcac gcccgtgacg acgaatggcg cgagcaatcc
     4921 ccactcgccg cgctgccgcg cgtcgcgcag ggctgcctcg cagtgctcga tcgcggcctg
     4981 cgggtcgccc gccatgagcg ccgcctcggc gaggccccac tggggtggca gcacgtattc
     5041 gagccagccg gccttccggc cgtattcgat cgacggttgg agatgcgact ccgcctcggc
     5101 tcgatcgccg cggcccgccg cgatccaccc gagcacccag cgcgcgatcg ccctggagcg
     5161 ggccgatccc gggtcgctcg ccgcctggcc gccctgctcc gtcgcctcgg cccagcgtcc
     5221 gtcggcccac gagaccatcg cctgggtcga ggcgaggagg tggccgcaga aggactgctc
     5281 gatctcctcc gcgtacttca gcccctcgcc gatctggcgt cccgccttcc ggtagtcgag
     5341 tgaccgcgtg gcgtaccacg ccgcgtcccg gtaggcgttg acggcgtgcg tctcgtggcc
     5401 ggcggatcga gctcggtcgg cgctttcgat caggatcgtc agcgccggct ccgggcgccc
     5461 ttcgagcacg tcgagcatcg cgagctcggt agcggcgtgt tcggcatcct cctcgagccc
     5521 ccacttgact gccgcgtcgc gcgattcgtc gaagagggcc ctcgcgtcgg caaagtggag
     5581 atcatcgacc tcggcgatgg cgaggaagtc gagcgctgcc acgtgcagga ccctcgccct
     5641 ctcgccctcg ggaagccgct cgacctcgag tgcgaggcgg cgggagagat cgcgaaactc
     5701 gacgacggat tcgccttccc ggcgggtgac gatcgcgatg ttgacgagtg cctcggcggc
     5761 ggcggtcgca tcgccggccc ggagggcgag ctcgcgactc tggactgcag cgtcccggca
     5821 gatgtcgttg cgatccaccg caccggcagc gtccgaatag ctcatccaga cccgtgcctt
     5881 ttcgacgtcg ggcaacgtcg tcggcatgtt ctggaccgcg cgccggtaca ggtcgaacgc
     5941 ctcgcgattc gaatacaccc gcttggcagc ttcggcagcg tcgagagcgg cccggaacgc
     6001 ctggtcgtgc agcccagcgc gctcgaaatg cagtgacgcg tcggtcgacc cgacgagcga
     6061 cgcgccgaac tctgcggctc gagcgtggaa tcggcgcagg tcgcgtgccg ggatgtcgtc
     6121 gtagagcgcg tctcgcagga gctgatgccg gaagtcgaaa tagccctcgt cgacgtagtc
     6181 gaatgggaag aggatcccgg cattgacgag ctcctcgagc ggctcgtcga gggccgcgag
     6241 cgggcgatcg aggattcccg cgaggacgtg tggcacgaag catcggccga gcacggcgcc
     6301 ggcgcgcgca accgcgcgag cctcgtcgct cagccgcgtg gcgtgggcca ggatcgcgtc
     6361 ctcgatcgtg tccggaacgt gggcgcccag gatcgagcgc ccatcagtcg tgctgccgcc
     6421 gaccgcggcc aggagttcct cgacgtggag cgggatcccg ttcgtccggg catgcactgc
     6481 ttcgaccacc tcgcgagagg ccggaagccc ggtgccgaga aggagggtcg tgacgagcgc
     6541 ggtctgatcc ttcgtcagcc gctgtacgcg cgcctcctca gcgagccgct gggtcaggag
     6601 gcgagcccgc cactcgcgat ggatcgatgc tgccggaagc tcgtccaacc ggtagacccc
     6661 gaccagcagc agcggcagct cgcgtccgcc tcgagccagc tccgcgatga cctcgagggt
     6721 catctcgtcc gcccactgca gatcctcgaa ggccaggagt gtcggctggt cgacggtccc
     6781 gagcatcatc tcgacaatgt cgcgcacgag gccgcggcgc gatccgagcg agtccgggcc
     6841 ctgcgcccga tcgaccccga gaagacgcgg gccgacgtcg ccccagtccc gggcgtccat
     6901 cgagcgcgcc agatcctgga t
//
LOCUS       DATBSW010000212         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1077276, whole genome shotgun sequence.
ACCESSION   DATBSW010000212 DATBSW010000000
VERSION     DATBSW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077276"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VIF63_05925"
     CDS             <1..1087
                     /locus_tag="VIF63_05925"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HEY8238954.1"
                     /translation="TPNPGPVATGPAPTESTTGTGGGTGGATPSFPTFDQSVLGITDV
                     NMEVPAVGGSLLLALLLLLIIAFAGELFNNTVENNYSIIAAWFKKGPFGWLRRMGGKL
                     GGEARVGLLIFLALTALISSFVDPHFGFDLRSLAEFLGFLFGLIVVLASFKLPMMLAH
                     KRKTGELGKLRPLPWALIIAALFVLISRIGNLQPGYLYGIVLGAIFVKDVEDKEEGRE
                     TFYGSFWTLVAAVVSWVGLNWVRGLGFAEDGFGVTVLSTAFAVTLVAGLEAAAFGLMP
                     MRFMPGHMVYKWNRLGWALLFGLSVFAFVHLLIGPTSGYVSELAPEAFLAALGVFAAF
                     GALSIVTWLYFRFRGESHRRMESAGPG"
     gene            complement(1095..1481)
                     /locus_tag="VIF63_05930"
     CDS             complement(1095..1481)
                     /locus_tag="VIF63_05930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8238955.1"
                     /translation="MTHVSRNPEEIPAPLGRYVHQIEVTDPGRILFISGQIGARADGS
                     VPADPVDQLDLALEYVIANLTAAGLGVEALTKITTFAVGELDPAARRAVLDKHLGTHV
                     SCSTLVFVAALARPEYKVEVDAWAAA"
     gene            complement(1492..2874)
                     /locus_tag="VIF63_05935"
     CDS             complement(1492..2874)
                     /locus_tag="VIF63_05935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8238956.1"
                     /translation="MPGTVAAVMGAWGIATPSLNQDEVVTMAAATRSAEQLARTVVNV
                     GSVFPAYFLGIHEWAQLAGTSHLALRAPSLLAMIGTASLVSALGQRADGARAGLLGGV
                     LFALLPSASFYAQTARSNSLAVFFVAASTLLLLVALDKRTRRWWVAYAAAVTLTGWSH
                     LFALSALASHAVVIAVVTRSQRPPVWKSWLASVLAASTIVAPVAALGIAKAAQIDWLP
                     LTSADLLGRSVGALIGAPLVAAVVAALALLGVGRIRDRPTAWLLGSLAFVPFTLLFVA
                     GSFAHVFHDRYVVFTVVAWSVLAGIALARARAPMALAALGLVVVLSASTYVAARQSGS
                     HGDGGLDTVAAEIARAAEPGDAIVYAPMGWLRLGIDYYLPSEGRPRDAYLLVRPSERD
                     TFTAAECADRRRCLDSAPRVWVVCIGAPSLALDCLGVGAADPLRADYVAGPTSVHDTL
                     SVTLFTRKSN"
     gene            complement(2982..3569)
                     /gene="lepB"
                     /locus_tag="VIF63_05940"
     CDS             complement(2982..3569)
                     /gene="lepB"
                     /locus_tag="VIF63_05940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8238957.1"
                     /translation="MSDLEQPRARKRAAGRLIELVQTIVLTVVIFLVIQTFVARPFKI
                     EGGSMETTLLPNQYVLIDKITPHWARYERGDIIVLEPPRAEVGGDQTPFIKRVIGLGG
                     DRIELHSGKVFVNGTVIDEPYIYTENGIQQPTDPTPGGLSEWLVPDGELFVMGDHRQN
                     SADSRTFGPIEVAHVIGRAWLRYWPIDTFGVVAPS"
     gene            complement(3562..>3870)
                     /locus_tag="VIF63_05945"
     CDS             complement(3562..>3870)
                     /locus_tag="VIF63_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238958.1"
                     /translation="QVLARSRSIVTANLAVLDEFFARRADAFTWVRSRGGSTGFPRLV
                     ADGPAGSSSDSLAARLVEATGVLLVPSSAFGSGDSHVRIGLGRTDLPEAVAKLDRFLV
                     "
BASE COUNT          628 a         1302 c         1330 g          610 t
ORIGIN      
        1 gacaccgaac ccgggtcccg tcgcgaccgg ccctgcgcca accgagtcga cgacaggcac
       61 gggcggcggg accggcggtg ccacgccgtc gttcccgacg ttcgaccaat cggtcctcgg
      121 gatcaccgac gtcaacatgg aggtaccggc agtcggcggg agcctgctgc tggctctgct
      181 cctgctcctg atcatcgcgt tcgccgggga gctgttcaac aacaccgtcg agaacaacta
      241 ctcgatcatc gccgcctggt tcaagaaagg cccgttcggc tggctgcgcc ggatgggcgg
      301 caagctcggc ggcgaggctc gcgtcggcct cctgatcttc ctcgcgctga cggcgttgat
      361 cagctcattc gtcgacccgc acttcggttt cgacctgcgc tcgctcgccg agttcctcgg
      421 cttcctcttc gggctcatcg tcgtcctggc gagcttcaag ctgccgatga tgctcgccca
      481 caagcgcaag accggcgagc tgggcaagct gcgaccgctg ccctgggcac tgatcatcgc
      541 ggcgctgttc gtgctgatct cgcgcatcgg caacctccag ccgggctacc tgtacgggat
      601 cgtgctcggc gcgatcttcg tgaaggacgt ggaggacaag gaggagggcc gggagacctt
      661 ctacggctcg ttctggacgc tcgttgccgc ggtcgtctcg tgggttggcc tgaactgggt
      721 ccgagggctc gggttcgcgg aggacgggtt cggcgtgacg gtgctgtcga ccgccttcgc
      781 ggtgaccctc gtcgcgggcc tcgaggcggc cgcgttcggt ctgatgccga tgcggttcat
      841 gcccgggcac atggtctaca agtggaaccg gctcggctgg gccctgctgt tcgggctcag
      901 cgtgttcgcc ttcgtccacc tgctcatcgg cccgacctcg ggctacgtat cggagctcgc
      961 ccccgaagcg ttccttgcgg cattgggtgt gttcgccgcg tttggcgcgc tgtcgatcgt
     1021 gacctggctg tacttccgct tccgcggcga gtcccaccgc cgcatggaaa gcgccggacc
     1081 gggctgaccg ttcgtcaggc ggcggcccag gcgtcgacct cgaccttgta ctcgggtcgt
     1141 gcgagcgcgg caacgaacac cagggtgctg cacgacacat gcgtgccgag gtgcttgtcg
     1201 agcacggcgc gcctcgcggc ggggtcgagc tcgccgaccg cgaacgtcgt gatcttcgtg
     1261 agcgcctcga caccgagccc tgcagcagtg agattggcga tgacgtactc gagcgcgaga
     1321 tcgagctggt cgaccgggtc ggccggcacg ctgccgtcgg cgcgggcgcc gatctgcccc
     1381 gagatgaaca ggatccggcc cggatctgtc acctcgatct ggtgcacgta gcggccaagc
     1441 ggggccggga tctcctccgg gtttcgactg acgtgcgtca cttgacctcc tctagttcga
     1501 tttcctcgtg aagagtgtga ccgacagcgt gtcgtggacc gaggttggac cggcgacgta
     1561 gtcggcccgg agcggatcgg ctgcaccaac gccaaggcaa tccaacgcaa ggctcggcgc
     1621 accgatgcaa accacccaga cccgaggcgc gctgtccagg caccggcgcc ggtcggcgca
     1681 ctcggcggcg gtgaaggtgt cgcgctccga cggccggacc aaaagatagg cgtctcgcgg
     1741 ccggccctcg cttggcaggt agtagtcgat cccaaggcgc agccagccca tcggcgcata
     1801 cacgatcgcg tcaccgggct cggccgcccg cgcgatctcg gccgccaccg tgtccaatcc
     1861 gccgtcaccg tggctgcccg attgtcgggc ggccacgtac gtcgacgcgg agaggacaac
     1921 gacgaggccg agcgcagcga gcgccatggg cgctcgggct cttgccagcg cgattccggc
     1981 gagaaccgac caggcgacca cggtgaacac gacataccga tcgtggaaca cgtgtgcgaa
     2041 tgagcccgcg acgaagagga gggtgaatgg aacgaaggcg aggcttccga gcaaccatgc
     2101 tgtcggccga tcacggatgc ggccgacgcc gagcagggcg agcgccgcga caacggcggc
     2161 aacgaggggc gccccgatga gcgccccgac ggacctcccg agcaggtcgg cgctcgtcag
     2221 cgggagccag tcgatctggg cggctttcgc aataccgagc gccgcgaccg gggcgacgat
     2281 cgtggacgcc gcaagcaccg atgccaacca cgacttccac accggcgggc gttgagaccg
     2341 cgtcacgact gcgatcacca cggcatggct ggcgagcgcg gatagcgcaa acaggtggct
     2401 ccagcctgtg agggtcactg ctgcggcgta ggcaacccac caccgcctcg tgcgcttgtc
     2461 gagggcgacg aggagcaaca gcgtcgatgc agcaacgaag aagacagcca ggctgttcga
     2521 ccgggccgtc tgcgcataga acgaagcgct cggcaacaag gcaaagagca ctcccccgag
     2581 gagtccggcc ctggcgccgt cggcgcgctg tcccaacgcg ctcaccagcg acgccgtgcc
     2641 gatcatggcg aggagcgatg gggcgcggag cgcaaggtgg gaggtgcccg cgagctgcgc
     2701 ccattcgtgg atcccgagga agtacgctgg gaagactgag cccacgttga ccacggttcg
     2761 cgccagctgc tcggccgacc tcgtcgccgc cgccatcgtt acgacctcgt cctggttgag
     2821 actcggcgtc gcgatgcccc aggcacccat gacggcggcg accgtccccg gcacgagaat
     2881 cggcgcccac ctcttcgcga gggccgtcgc ccgtgcccgt cgggcgtgct ttggtggaga
     2941 gtcctgcgtg aggtcggtgc ccaaggcgag gagcactccc ttcagctcgg cgcgacgact
     3001 ccgaaggtgt cgatcggcca gtagcggagc caggcgcgcc cgatcacgtg ggcgacctcg
     3061 atcggaccga acgtcctcga atccgccgag ttctggcgat ggtcgcccat cacgaagagc
     3121 tcgccgtcgg ggacgagcca ttccgacagc ccgcccggcg tagggtcggt cggctgctgg
     3181 atgccgttct ccgtatagat gtacggctcg tcgatcaccg tgccgttgac gaagaccttc
     3241 ccgctgtgca gctcgattcg gtcgccgccg aggccgatga cgcgcttgat gaacggggtc
     3301 tggtccccgc cgacctcggc ccttggcggc tcgagcacga tgatgtcgcc acgctcgtac
     3361 cgggcccagt ggggcgtgat cttgtcgatc agcacgtact ggttcggcag gagcgtcgtc
     3421 tccatcgagc caccttcgat cttgaacggg cgcgcgacaa acgtctggat gacgaggaag
     3481 atcacgacgg tcaggacgat cgtctggacg agctcgatga gacggccggc cgcgcgcttc
     3541 ctggcccgtg gttgctcgag gtcagacaag gaatcggtcg agcttcgcca cggcttcggg
     3601 caggtcggtt cggccgaggc cgattcggac gtgcgaatcg ccactcccga acgcactcga
     3661 gggcaccagc aggacgcccg tggcctcgac gaggcgcgcc gccagtgaat ccgacgacga
     3721 acctgcgggt ccatcggcga caagtcgcgg gaagcccgtc gagccgccgc gcgagcgtac
     3781 ccacgtgaac gcgtccgcgc gccgcgcgaa gaactcgtcg aggacggcga ggttcgccgt
     3841 gacgatcgat cggctgcgag ccagcacctg
//
LOCUS       DATBSW010000213         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1077726, whole genome shotgun sequence.
ACCESSION   DATBSW010000213 DATBSW010000000
VERSION     DATBSW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077726"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VIF63_05950"
     CDS             <1..1031
                     /locus_tag="VIF63_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8238959.1"
                     /translation="MRLVTDVFEFGARELPKWNTISISGYHMREAGATAAQELAFTLA
                     DAIAYVEAALARGMAIDDFAPRLSFFFAAWSELFEEVAKFRAARRMWARIVKERFGSS
                     NARSMACRFHVQTAGSSLTAQSVDNNVVRTTVQALAAILGGAQSLHTNSRDEALALPT
                     EDSVRLALRTQQILAHESGVSETPDPMGGSYFVEALTSELEAAATAYLDEIDAMGGTM
                     RALEAGFQQRSIQESAYRVQRAIDSGEQVVVGVNAFRDEAVAAPPTQKIDPEAEARQV
                     AGVRRVRAERDPAAWEAAMRRLEDAARGTDNLLPPIIEAVKAYATVGEIANRLRAAWG
                     VHRELITV"
     gene            1055..1531
                     /gene="mce"
                     /locus_tag="VIF63_05955"
     CDS             1055..1531
                     /gene="mce"
                     /locus_tag="VIF63_05955"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY8238960.1"
                     /translation="MAVRRAERPSVRFKPPGRIHHVAIVVASIETSLAVYRDLLGLPL
                     ETIMDIESDRVRIAFLGIGESKIELVQPTDDTTGVARFLASKGEGFHHVCFEVANLAE
                     ELTRLGIDGVELIDSAPRRGAEGPVAFLHPRSCHGVLVELIEQPGGPSWMSLGYTP"
     gene            1544..2521
                     /locus_tag="VIF63_05960"
     CDS             1544..2521
                     /locus_tag="VIF63_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8238961.1"
                     /translation="MRTFLFLALRTPELTARLVRAAARSRATVVLDLEDALWDVTDPA
                     RTAELKATGRATLVALARDHPELFRRQPVGVRVNRVAGDDAEQDFEAIERVSALVPVA
                     CVVIPKVESADDIAAARTRIRRLGLPPEAIVPILETRSALAALDSLLPAARSAGVEWI
                     VFGFFDFALDSGWWPFPDRHGDELWAVIRPFLERVEDAGMRYIEAPYAQVDDDAGLVR
                     ILDRLERTCDREFGVLTVGLGQASVAQRFAAGRALVRVGSAVGAPGHAGVAAGPVRDA
                     LDQALRVVEVYDARHRPDTSFVFDPETGQFIPPHVYLGARRYLAAHHRA"
     gene            2562..3356
                     /locus_tag="VIF63_05965"
     CDS             2562..3356
                     /locus_tag="VIF63_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238962.1"
                     /translation="MTHQRGVPLNALYHRQLAGLLAERPGIKLRTHVVRAFDADLASR
                     LELLLEDAPVDGVLVHLRAAPLVWSVRIVRRAWRDGQFQTELNPALNPALFRGTAAGA
                     GTQAFGSGVEDDDAYGGAPDLQDRPPTGRRIAGMRVRNLNVAAGTIVGLDRIAIRRQL
                     REFAALERAARARGVSLFVLGPTPTTYSWWTNRLVRKAEAVIRQALTGTPVPFALVES
                     AYDDAGRPVIRADGTHLTIDGHRYVAGRLERAGLGDWMSRILAARA"
     gene            complement(3361..3831)
                     /locus_tag="VIF63_05970"
     CDS             complement(3361..3831)
                     /locus_tag="VIF63_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238963.1"
                     /translation="MALVMDLVAGDSREILLAIAVDDLASFDDRSRFPAHLTFGGAID
                     PTWLDLFSQAVRSALGRDEPRDFLDARVELDGPGDAGERTVERIDPTWITAIARLGDR
                     DLDAIAGRWIDLLEDELGHLPREEKPTIRIFARQIVEFARAADLAPAVILAWSL"
     gene            complement(3841..4512)
                     /locus_tag="VIF63_05975"
     CDS             complement(3841..4512)
                     /locus_tag="VIF63_05975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8238964.1"
                     /translation="MADPDAKHGSASSDDEQKPRSFDERAVDWDTPERVARAKVAAET
                     IRGAVEFPHGARAIDVGAGTGLLGLALLDRLGELVLADTSDGMIEQAQRKIAEGKLEN
                     VRAIHLDLIADPPPDGAPFDAVVSQLLLHHVEDTQAALSSMHRLLVPGGQLVALDLDT
                     EDGSFHGSVGTSVHHLGFDREALVVLARSVGFLDVHVGAGGMIGDAERGEELFPMFLL
                     TARRG"
BASE COUNT          632 a         1627 c         1628 g          656 t
ORIGIN      
        1 cgatgcgcct cgtgaccgac gtcttcgagt tcggcgctcg cgagctgccc aagtggaaca
       61 cgatctcgat ctcgggctac cacatgcgcg aggcgggggc gacggcggcc caggagctgg
      121 ccttcaccct cgccgacgcg atcgcgtacg tcgaggcggc cctcgcccga gggatggcga
      181 tcgacgactt cgcgccgcgc ctctccttct tcttcgccgc gtggtcggag ctgttcgagg
      241 aggtcgccaa gttccgcgcg gcccgccgga tgtgggcgcg gatcgtcaag gagcgcttcg
      301 ggtcgtcgaa cgccagatcg atggcctgcc ggttccatgt ccagaccgcg ggttcgtcgc
      361 tcaccgccca gtcggtcgac aacaacgtgg tccgcacgac cgtgcaggcg ctcgccgcga
      421 tcctcggcgg cgcccagagc ctgcacacga actcgcggga cgaggcattg gccctgccga
      481 ccgaggacag cgtgcggctg gccctccgga cccagcagat actcgctcac gagtccggcg
      541 tgtccgagac ccccgatccc atgggcggct cgtacttcgt ggaggccctc acctccgagc
      601 tcgaggcggc cgcgaccgcc tacctcgacg agatcgacgc gatgggcggc acgatgcgcg
      661 ccctcgaggc gggcttccag cagcgctcga tccaggagtc ggcgtaccgc gtgcagcggg
      721 caatcgactc gggcgagcag gtcgtggtcg gcgtgaacgc gttccgcgac gaggccgtcg
      781 cggcgccgcc gacccagaag atcgatcccg aggcggaggc gcgccaggtc gccggcgtcc
      841 ggcgcgtccg cgccgagcgc gacccagccg cgtgggaggc agcgatgcgc cggttggagg
      901 atgccgcgcg cgggacggac aacctgctgc cgccgatcat cgaggcggtg aaggcctacg
      961 ccacggtcgg cgagatcgcg aaccgcctgc gggccgcatg gggcgtccat cgcgagctga
     1021 tcacggtctg acgagcagcg gcggaggagg agacgtggcc gtacgaagag ccgagcgacc
     1081 gtcggtccgg ttcaagccgc ccgggcggat ccatcacgtc gcgatcgtgg tcgcgtccat
     1141 cgagacgtcc ctggccgtct atcgcgacct gctcgggctg cccctcgaga cgatcatgga
     1201 catcgagtcc gatcgtgtcc ggattgcgtt cctcgggatc ggggagtcga agatcgagct
     1261 cgtccagccg accgacgaca ccaccggcgt cgcgcgcttc ctcgcgagca agggcgaggg
     1321 ctttcaccac gtctgcttcg aggttgccaa cctcgccgag gagctgactc gcctggggat
     1381 cgacggcgtt gagctgatcg attccgcgcc gcgccgcggc gccgagggcc cggtcgcgtt
     1441 cctgcaccca cggtcctgcc acggcgtgct cgtcgagctc atcgagcaac cgggcggccc
     1501 atcctggatg agcctcggct acaccccgta gccaccgatt caggtgcgga ccttcctgtt
     1561 cctggcgctc cggacccccg agctgacggc caggctcgtc cgagccgccg cccggagccg
     1621 cgcgacggtc gtcctcgacc tcgaggacgc gctctgggac gtgactgatc cggcacgaac
     1681 ggccgagctc aaggcgaccg ggcgcgcgac cctcgtcgcg ctggcccgcg accaccccga
     1741 gctgttccgg cggcagcccg tcggtgttcg ggtcaaccgg gtggccggcg acgacgccga
     1801 gcaggacttc gaggcgatcg agcgcgtatc ggcgctggtc cccgtggcgt gcgtcgtcat
     1861 ccccaaggtc gagagcgcgg acgacatcgc cgccgctcgc acccggattc gccggcttgg
     1921 cctcccgccc gaagcgatcg tcccgatcct cgaaacccgc tccgccctgg ccgcgcttga
     1981 ctccctcctc cccgcagcgc gatcggcggg ggtcgaatgg atcgtcttcg gcttcttcga
     2041 tttcgccctg gattccgggt ggtggccgtt cccggaccgt cacggtgacg agctctgggc
     2101 ggtgatccgt ccgttcctcg agcgggtcga ggacgcgggc atgcgctaca tcgaagcgcc
     2161 gtatgcgcag gtcgacgacg atgccggcct ggtccggatc ctcgaccgcc tcgagcgcac
     2221 ctgcgaccgc gagttcggcg tgctcacggt gggcctcggc caggcgagcg tggcccagcg
     2281 gttcgcagcg ggccgcgccc tggtcagggt ggggagcgcc gtcggagcgc ccgggcatgc
     2341 cggcgtggcg gccgggcccg tgcgcgacgc gctcgatcag gcgcttcggg tcgtggaggt
     2401 gtacgacgct cggcaccgac cggacacgag cttcgtgttc gaccccgaga cggggcagtt
     2461 cattcccccc cacgtgtacc tgggggcgcg gcgatatctc gcggcgcacc accgtgcctg
     2521 agccgcggcc cgagtttcgg ctcggcatca tcggtggctg catgacccac cagcgcgggg
     2581 tgcccctcaa cgcgctctac caccggcagc tcgccggcct gctcgccgag aggcccggca
     2641 tcaagctgcg aacccacgtc gtccgggcgt tcgacgccga cctcgcgtcc cgcctcgagc
     2701 tgctcctgga ggacgcaccg gtcgatggcg tgctcgtcca tctccgtgcg gccccgctcg
     2761 tctggtcggt tcgcatcgtt cgtcgggcgt ggcgcgacgg gcagttccag acggagctca
     2821 acccggccct caacccggcg ctgttccgag gcacggcggc cggcgccggc acgcaggcat
     2881 tcgggtccgg ggtcgaggac gatgatgcct acggcggcgc gcccgacctc caggaccggc
     2941 cgccgaccgg ccgccggatc gcgggcatgc gcgtccgcaa cctcaacgtg gccgcgggga
     3001 cgatcgtcgg cctggatcgg atcgcgatcc ggcgacagct gcgcgagttc gccgcactgg
     3061 agcgagctgc tcgcgcgcgc ggggtctcgc tcttcgtgct cggtccgacg ccgacgacct
     3121 attcctggtg gacgaaccga ctcgtgcgca aggcggaggc cgtgatccgg caggcgttga
     3181 ccggcacgcc cgtgccgttc gcgctggtcg aaagcgccta cgacgatgcg ggccgcccgg
     3241 tgattcgcgc cgatggcacc catctcacca tcgacggcca ccggtacgtc gcggggcgcc
     3301 tcgagcgggc gggcctgggc gactggatgt cgcgaatcct cgcagcgcgg gcctgaccgg
     3361 tcagagcgac caggcgagga tcaccgccgg cgccaggtcg gcggcgcgag cgaactcgac
     3421 gatctggcgg gcaaagatcc gaatcgtcgg cttctcctcg cgcggcaggt gccccagctc
     3481 gtcctcgagg agatcaatcc agcggccggc gatggcatcg aggtcgcgat cgccgagccg
     3541 ggcgatcgcg gtgatccagg tggggtcgat ccgctcgacc gtgcgctcgc cggcgtcgcc
     3601 ggggccgtcg agctcgaccc gggcgtcgag gaagtcgcgc ggctcgtcac gcccgagcgc
     3661 ggagcgcacc gcctggctga acaggtcgag ccaggtcggg tcgatcgccc cgccgaacgt
     3721 gaggtgcgcc ggaaagcgcg agcgatcgtc gaagctcgcc aggtcgtcga cggcaatcgc
     3781 gaggaggatc tcgcgggagt caccggccac caggtccatc acgagggcca cggcgaccgc
     3841 ctagccccgc cgggcggtga gcaggaacat cgggaacagc tcctcgccgc gttcagcgtc
     3901 gccgatcatc ccgcccgcgc cgacgtgcac gtcgaggaag ccgacggagc gggccagcac
     3961 gacgagcgcc tcccggtcga agccgaggtg gtggacgctc gtgccgaccg agccgtggaa
     4021 cgagccatcc tcggtgtcga ggtcgagggc cacgagctga ccaccgggca ccaggagccg
     4081 gtgcatcgag gacaacgccg cctgggtgtc ctccacgtga tgcaggagca gctgcgagac
     4141 gaccgcatcg aacggggcgc cgtcgggggg cgggtcggcg atgaggtcga gatggatcgc
     4201 gcggacattc tcgagcttgc cctcggcgat cttgcgctgc gcctgctcga tcatgccgtc
     4261 cgaggtgtcg gcgagcacga gctcccccag ccggtcgagg agcgcgaggc cgaggaggcc
     4321 ggtgccggcg ccgacgtcga tcgcacgggc gccgtgcggg aactcgaccg ccccgcggat
     4381 ggtttcggcg gcgaccttcg cccgggcgac gcgctccggc gtgtcccagt cgaccgcccg
     4441 ctcgtcgaag ctgcgcggct tctgctcgtc gtccgacgat gctgatccgt gcttcgcgtc
     4501 gggatcagcc atttcgcgct agccgctgta gccggccgcc cgc
//
LOCUS       DATBSW010000214         5107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_478889, whole genome shotgun sequence.
ACCESSION   DATBSW010000214 DATBSW010000000
VERSION     DATBSW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478889"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VIF63_05980"
     CDS             complement(<1..475)
                     /locus_tag="VIF63_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8238965.1"
                     /translation="MTDISIQAPTHQAIYRRWRAQTFAQVVGQEAVVETLRNAVRTER
                     VAHAILFVGPRGTGKTSLARILAKAINCTNLQDGDPCDACSSCVAIREGRAMDLLEID
                     AASNRGIDAIRDLRERINYAPTDLRRKVYILDEAHQITKDAWNALLKSLEEPPDFV"
     gene            complement(509..772)
                     /gene="ffs"
                     /locus_tag="VIF63_05985"
     ncRNA           complement(509..772)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIF63_05985"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(796..1491)
                     /locus_tag="VIF63_05990"
     CDS             complement(796..1491)
                     /locus_tag="VIF63_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY8238966.1"
                     /translation="MTRARAIDVVALPMGEFTFPAGEEYEGQTGLVVAYAIRHAAGTF
                     LFDTGFAMPDPELDNFYLRWDIRPSSLPEVASAAGLDLAEVTAVANCHLHLDHAGQNP
                     LFPGVPIYVQGPEWDIAHEPDYTFLPAIDFQGANYQHRAGDHEVAPGIRIFATPGHSP
                     GHQSVVVDTPDGPLLLAGQAVYSYGEWSGIPGTREGATTARDGEAYARSIARLKALNP
                     KQVLFGHDRQGWP"
     gene            complement(1737..1824)
                     /locus_tag="VIF63_05995"
     tRNA            complement(1737..1824)
                     /locus_tag="VIF63_05995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1788..1790),aa:Ser,seq:gga)
     gene            complement(1895..1980)
                     /locus_tag="VIF63_06000"
     tRNA            complement(1895..1980)
                     /locus_tag="VIF63_06000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1944..1946),aa:Ser,seq:cga)
     gene            complement(2018..2458)
                     /locus_tag="VIF63_06005"
     CDS             complement(2018..2458)
                     /locus_tag="VIF63_06005"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY8238967.1"
                     /translation="MNYELYMGEALAEAKAAIARGERPHAAVAVLDEAMVARGHDRVR
                     ESDDPTAHAVIVALREAARRVGKDRLRDVTMFCTMEPCPMCVGALLACDVEALVYALP
                     NRTDGAAGTILQLAEHPGLPRRIKVVSGIRRDEVEELYATAALR"
     gene            2518..3699
                     /locus_tag="VIF63_06010"
     CDS             2518..3699
                     /locus_tag="VIF63_06010"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY8238968.1"
                     /translation="MADLRRIGVLTGGGDVPGLNSVIKSVTYRATELGIEVIGIRRGW
                     EGLTHVQPGGDLDPEYVRVLNRSNTRTIDRTGGTMLHTSRTNPAHMKEKSLPPWLDAA
                     KAESYRTDEGRYDLTDVVLEHLDALGIDALVPIGGDDTLSFARVLVDRGVHLVAIPKT
                     MDNDVPGTEYCIGFSSAITRAKELIDRQRTTLGSHERIGVFRIFGRDAGFSALFAAYV
                     TSARCVIPESPYDIDPLAALLADDHRLSPSHYAILVAAEGAIWAGASLEGVGEPDAFG
                     HRHKANVGEALGIELTKRTGIESLSIELTYDLRSGQPDALDQIVATTFANVAMDLVAD
                     GRFARMVAIRDGKYAETSLPAAGSKARRVDVAAMYNTERFRARYDGRIGRPMLLGDLA
                     G"
     gene            complement(3717..4595)
                     /locus_tag="VIF63_06015"
     CDS             complement(3717..4595)
                     /locus_tag="VIF63_06015"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8238969.1"
                     /translation="MRVGFVGLGTMGGPMAANAARAGFEVSAWNRTPGRATDLEALGV
                     KLLDSPLAVAAASEIVITMVSDTPDVEAVLFGPGGVAEGAKAGTLVVDMSTISPSATR
                     QFAERLGASGVAMLDGPVSGGSEGAKKGTLSIFIGGDVADLERARPVLQSLGTTITHV
                     GPIGAGQAVKAVNQVILAGTYIGVAEGIVLALKAGLDVEQTVAALSGGAAQSWVLTNR
                     SGRMLANDYPLGFKVALHRKDLGIALSMARELGASLPISALVEAIEAGLIGQGHADDD
                     MSAVARSIRALSGLES"
     gene            complement(4727..>5107)
                     /locus_tag="VIF63_06020"
     CDS             complement(4727..>5107)
                     /locus_tag="VIF63_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238970.1"
                     /translation="EPGVGRTASAITDWLTSLPGLDASAPEPVTVGGLPGFSVDLTLA
                     AAWTHGCDYSDGKPIVSTFVDSDDSAEGFDWNVGAVGKVRYIILDLGDGRALLIDIEV
                     ADIADFDGLVADAMPVIGTFTFRK"
BASE COUNT          781 a         1730 c         1834 g          762 t
ORIGIN      
        1 cgacgaagtc gggcggctcc tcgagcgact tgagcagcgc gttccaggcg tccttcgtga
       61 tctggtgggc ttcgtcgagg atgtagacct tgcggcgcaa gtcggtcggg gcgtagttga
      121 tgcgctcgcg gaggtcgcgg atcgcgtcaa tgccgcggtt cgacgcggcg tcgatctcga
      181 ggaggtccat cgcccggccc tcgcggatcg cgacgcatga cgagcaggcg tcgcacgggt
      241 cgccgtcctg gaggttcgtg cagttgatcg ccttcgccag gatccgggcc agcgacgtct
      301 tgcccgtgcc acgcggcccg acgaacagga tcgcgtgtgc gacccgctcg gtgcggacgg
      361 cgttgcgcag ggtctcgacg acggcttcct gcccgacgac ctgggcgaac gtctgggcac
      421 gccagcgccg gtagattgcc tggtgggtcg gcgcctggat cgagatgtct gtcacgcagc
      481 tccgtgcctg agagggctgg gtcaaatgcc gtgcaccacc tgtcgaccga ctggcgcccg
      541 ggcccaccgc agaaggcggc tcggatcagg ccgttccgcg gcacccgacg aggttcgctg
      601 agtgctgctt ccttccggac ctgacacggt tcgcgattcg ccgctgcgcg ggacccgatc
      661 ctcaacgccg cctggagcgg cggcctccga gcgcagaggc ctcgaggagg aattcagccc
      721 tgctggagcg gattgcaggt acagggcacc gctagttccc cgcctagcac ggcccgccaa
      781 ggataccccg gtccgttacg gccagccctg tcggtcgtgg ccgaagagga cctgcttcgg
      841 gttcagggcc ttgagccgcg cgatcgaccg ggcgtatgcc tcgccatcgc gcgctgtcgt
      901 ggcgccctcg cgggtgccgg ggatgccgga ccactcgccg tagctgtata ccgcctgccc
      961 tgcgagcagc agcggtccgt ccggcgtgtc gacgacgacc gactggtgac cgggcgagtg
     1021 gcccggcgtc gcgaagatgc ggatgcccgg tgccacctca tggtcgcccg cgcggtgctg
     1081 gtagttcgcg ccctggaaat cgatcgccgg caggaacgtg tagtcgggct cgtgggcgat
     1141 gtcccactcg ggtccctgaa cgtagatcgg gacaccgggg aacagcgggt tctgccccgc
     1201 gtggtcgagg tggaggtggc agttcgcgac ggcggtgacc tcggcgaggt ccaggccggc
     1261 ggcggatgcc acctcgggca gggagctcgg ccggatgtcc cagcgcaggt agaagttgtc
     1321 gagctcgggg tcgggcatcg caaagcccgt gtcgaacagg aacgtgcctg ccgcgtgccg
     1381 gatggcgtac gcgaccacca gacccgtctg gccctcgtac tcctccccgg cagggaacgt
     1441 gaactcgccc atcggcaggg caacgacgtc gatcgcgcgg gctcgggtca tcgcctgctc
     1501 gcgggtgttg ggaccgcgta ttccgtcaga tacgcaccat cggcgtggta tccgccaaat
     1561 ggtcgccgct tgtggacgtc gcgcctcaac tccatcccgc cttgagcgtg ggccgggacg
     1621 aaaccgcggc ccaatcgcgc gaaactgttg atctggcctg gataccacgc atcgggtgtg
     1681 taggtggcgg aaggatgggc gcccgggacc ggcgtcgcgg tatcgaccgg atggtgtggc
     1741 ggagagggag ggattcgaac cctcgacggg ttgccccgaa ccgcatttcc agtgcggcgc
     1801 catagtccac taggcgacct ctccgcgaga cgcccgagtc tagagggcgc ggaggcgggc
     1861 aaacgccggc atgggctgcc cgctggtgag cgactggcgg agagggtggg attcgaaccc
     1921 acgctgcttt cgcagaccgc ttttcgagag cggcaccatc aaccactcgg acacctctcc
     1981 gccggggagg atagcccagc acgagccgag agggccgtca gcgcagggcg gcggtggcgt
     2041 agagctcttc gacctcgtcg cggcggatcc cgctgacgac cttgatccgg cgcgggaggc
     2101 cgggatgctc cgcgagctgg aggatcgtgc cggcggcgcc gtcggttcgg ttgggcagcg
     2161 cgtagaccag cgcttcgacg tcgcaggcca ggagcgcgcc gacgcacatg gggcagggct
     2221 ccatcgtgca gaacatggtc acgtcccgga gtcggtcctt gccgacacgc cgggcggcct
     2281 cgcggagagc aacgatgacg gcgtgggccg tcgggtcgtc cgactcgcgg acgcgatcgt
     2341 ggcctctggc gaccatcgcc tcgtcaagca cggcgacggc cgcgtggggc cgttcgcccc
     2401 gggcgatggc ggccttcgcc tcggccaggg cctcgcccat gtacagctcg tagttcacgc
     2461 ggcggatgat acctgccggt gccggcgctc cccgcgttgc cgataccatc accgccgatg
     2521 gcagacctca ggcggatcgg cgtgctcacc ggcggcggcg acgtccctgg gctcaactcg
     2581 gtcatcaaga gcgtgaccta tcgggcgacc gagctcggca tcgaggtcat cggcatccgg
     2641 cgcggctggg agggtctgac ccacgtccag cccggcggcg acctcgaccc tgagtacgtg
     2701 cgcgtcctga accgaagcaa cacccggacg atcgatcgga ccggcgggac gatgctccac
     2761 acgtcgcgga cgaacccggc gcacatgaag gagaagtcgc tcccgccctg gctcgacgcc
     2821 gccaaggccg agagctaccg aacggacgag ggtcgctacg acctgaccga cgtcgtgctc
     2881 gagcacctcg acgcgctggg catcgacgcc ctcgtcccga tcggcggcga cgacacgctg
     2941 tcgttcgcgc gcgtgctcgt cgaccgcggc gtccacctcg tcgccatccc caagacgatg
     3001 gacaacgacg tgcccgggac cgagtactgc atcggcttct cgtcggcgat cacgcgggcc
     3061 aaggagctga tcgatcgaca gcggacgacg ctcggcagcc acgagcggat cggcgtgttc
     3121 cggatcttcg gccgggacgc ggggttctcc gccctgtttg ccgcctacgt gacctcggcg
     3181 cggtgcgtca tcccggagtc gccgtacgac atcgatccgt tggcggcgct cctcgcggac
     3241 gaccaccggc tcagcccgag tcactacgcg atcctcgtcg ccgccgaggg cgcgatctgg
     3301 gccggcgcga gcctcgaggg cgtgggcgag ccggacgcgt tcgggcatcg gcacaaagcg
     3361 aacgtcggcg aggcgctggg gatcgagctg acgaagcgga ccgggatcga gtcgctgtcg
     3421 atcgagctca cctacgacct gcgaagcggc cagcccgacg cgctcgacca gatcgtcgcg
     3481 acgacattcg cgaatgtggc gatggacctc gtcgccgacg ggcggttcgc gcggatggtc
     3541 gcgatccggg acggcaagta cgcggagacc tcgctgccgg cggcgggttc caaagcccgt
     3601 cgggtcgacg tcgcggcgat gtacaacacc gagcgcttcc gcgcccggta cgacggccgg
     3661 atcggccgac ccatgctgct gggcgacctc gcggggtagg gcgggccagc cgcgggttag
     3721 gactccagcc cggacagggc gcggatcgaa cgggcgacgg cggacatgtc gtcgtcggcg
     3781 tgaccttggc cgatgagccc ggcttcgatc gcctcgacga gggcgctgat gggcagcgat
     3841 gcgcccagct cgcgggccat cgagagcgcg atgccgaggt ccttgcgatg gagcgcgacc
     3901 ttgaacccaa gcggatagtc gtttgcgagc atccggccgc tccggttcgt caggacccag
     3961 gactgcgcgg cgccgccgga cagggccgcg acggtctgct cgacgtcgag cccggccttc
     4021 agcgcgagga cgatcccttc cgcgaccccg atgtacgtgc ccgcgaggat cacctggttc
     4081 accgccttga ccgcctggcc ggcaccgatc gggccgacgt gggtgatcgt cgtgcccagg
     4141 ctctggagga ccggccgcgc ccgctccagg tcggcgacgt cgccgccgat gaagatcgac
     4201 agcgtgccct tcttcgcgcc ctcgctcccg cccgagacgg ggccgtcgag catcgcgacg
     4261 ccgcttgcgc cgaggcgctc ggcgaactgg cgggtcgcgg atggcgagat cgtcgacatg
     4321 tcgaccacga gcgtgccggc cttggctccc tcggcgacgc cgccgggacc gaacaggacg
     4381 gcctcgacgt cgggcgtgtc cgagaccatg gtgatgacaa tctcgctcgc ggcggccacg
     4441 gccaagggac tgtcaaggag cttgacgccc aatgcctcga ggtcggtcgc gcgaccgggt
     4501 gtccgattcc aggcgcttac ctcgaagccg gcgcgtgcgg cgttggcggc catcgggccg
     4561 cccatcgtgc cgagaccaac gaacccaacg cgcacctggt cctcctcgcc gcttcgccct
     4621 gctcagggat gctagcgcgc gacgcgtgcg acccggacgc gacgatgcgg gcgccgaagc
     4681 gcccgcatcg agaaaccgga gcggtggcgg tggagggccg ccagggtcac ttccggaagg
     4741 tgaaggtgcc gatgaccggc atcgcgtcgg ccacgaggcc gtcgaagtcg gcgatgtcgg
     4801 cgacctcgat gtcgatcagg agcgcgcgac catcaccgag gtcaaggatg atgtagcgca
     4861 ccttgccgac ggctccgaca ttccagtcga acccctccgc gctgtcgtcg gagtcgacaa
     4921 aggtggagac gatgggcttg ccgtcactgt agtcgcagcc gtgcgtccag gcggcagcga
     4981 gggtcaggtc aacgctgaac ccaggaagcc caccaacggt gacgggctcc ggcgccgacg
     5041 cgtcgagacc cggcagcgac gtgagccaat cggtgatcgc tgacgccgtc cgacccacgc
     5101 cgggttc
//
LOCUS       DATBSW010000215         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_126260, whole genome shotgun sequence.
ACCESSION   DATBSW010000215 DATBSW010000000
VERSION     DATBSW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126260"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VIF63_06025"
     CDS             complement(<1..632)
                     /locus_tag="VIF63_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238971.1"
                     /translation="MRTRLARLIGVAASAAVLASALAGAPAALAKITFSQGQTQGING
                     CSFFFPTFPQEGAGEKYACEQWSIDVPPNALDWDFHDKRSTPYTTSTGVSTTANIEET
                     SAIETTLDDDGEFAELHFESHFDASVENGMQAQGFMNYLLQLKTVAPKLTVTGTAHIE
                     STGMSRYPAANPNSQVKAAGLSGFVVLISCGTTVGQATEQMIAGGDEPLE"
     gene            complement(734..1117)
                     /locus_tag="VIF63_06030"
     CDS             complement(734..1117)
                     /locus_tag="VIF63_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8238972.1"
                     /translation="MARDPNIPDEFEVFTLVLLRRPADAPQMSDEELDALQARHLAYR
                     AELKRQGLLVANGPLGEQSDMRFRGMSIFACGLAEAARLSDGDPSVQAGRLAYDVMEW
                     WVAAGTLAFPNAPGPVGDRRVMPDD"
     gene            1247..1789
                     /locus_tag="VIF63_06035"
     CDS             1247..1789
                     /locus_tag="VIF63_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8238973.1"
                     /translation="MDRAAAGDREAFGALVRASAARLDGAARLILRDPDLARDAVQEA
                     LIRAWRDLPTLRDRDRFDAWLHRLLVNAAIDEARRRRRRVVEIDLDSVASPSTADTTS
                     RQADSDALDRVLQLLPPEHRALIVQHYYLGVPLPEAARILGISHDAAKQRLHRAIVAL
                     RQKFESQADNASISLEKGNA"
     gene            1786..3216
                     /locus_tag="VIF63_06040"
     CDS             1786..3216
                     /locus_tag="VIF63_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY8238974.1"
                     /translation="MSTADRFDRHLESLLAELTGPQMPAYIDDALATATARPQRGPSI
                     LSRFGAGTAGPRYRMALVGLPVVLVLAAAIVGLLVIGNQNPTPSPTPNAPSAGPTETS
                     EASPVPTTKPTPTPAPSLGTCDGYARAADARVVDAVGEQPWETEKVVSPPAFSSLGDG
                     LIAGFAEQLASGPAVVLIDPADGTACRLVDLESAADLWELEWSPLGDALAIAVGKQVL
                     VWSAAGLTQALRLADSGFGVTLAWSPTGEAIAIGSGSPIAVVFADGSRRSVAIQHATS
                     LAWSPDASRFAAIGVDTDDPEDTRRLVVISGDGLVIDPIDERAGLAGWLDGQTILAGS
                     STDAGALEAFDLGPRTWRTWSPASTEPIGLTSIIGYAPGLTGLAFFANEDINKLAAPM
                     DLIVRDLPSGERHVLATKAYPPSNAFGTGAWSPDGTHIAVSVIGDPTDPRARPGVSIY
                     ALAGGDPTFVSPIQVILTEGSWQPVP"
     gene            3240..>3729
                     /locus_tag="VIF63_06045"
     CDS             3240..>3729
                     /locus_tag="VIF63_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY8238975.1"
                     /translation="MAKYLVLLRGINVGGRNKVAMAALRTALERLGFENVSTYIASGN
                     VFLDSDLAASKVAAKIEAALPKAFKLDAELIRVAVLSAADLEAVVKRRPKGFGAEPAK
                     YHSDAVFLMGIDAAEAMSVFSPRDGVDTVWPGKGVIYSQRLSAERTRSRLNRIVGTPA
                     YRS"
BASE COUNT          569 a         1308 c         1276 g          576 t
ORIGIN      
        1 aactcgaggg gttcgtcgcc gccggcgatc atctgctccg tcgcctggcc aaccgtggtc
       61 ccgcagctga tgaggaccac gaacccgctg aggccggccg ccttgacctg ggagttcggg
      121 ttggccgccg ggtagcgcga catcccggtt gattcgatgt gggccgtgcc cgtgacggtc
      181 agcttcggtg caaccgtctt gagctggagg aggtagttca tgaagccctg ggcctgcatc
      241 ccgttctcga ctgatgcgtc gaagtgcgac tcgaaatgca gctcggcgaa ctcgccgtcg
      301 tcgtcgaggg tcgtctcgat cgcggacgtc tcttcgatgt tcgcagtggt cgagacaccc
      361 gtcgacgtcg tgtagggagt cgagcgcttg tcatggaagt cccagtcgag ggcgttgggc
      421 gggacgtcga tcgaccactg ctcgcacgcg tacttctcgc cggcgccctc ctgtggaaac
      481 gtcgggaaga agaagctgca gccgttgatg ccctgggtct ggccctgcga gaacgtgatc
      541 ttcgcgaggg cagcgggcgc gccagcgagg gccgatgcga gcaccgcggc gctcgccgcc
      601 acgccgatca gccgggccag ccgagtccgc atgcgaagtc cctcccgtcg cctcgtttcg
      661 cgccaggttt gtcgcgcatg gtagaggtcg cggaagtgct cctaccgggt gatttgccgc
      721 acggcggacc gcttcaatcg tcgggcatca cccggcgatc gccgaccggc ccgggggcat
      781 tcgggaacgc gagggtgcct gccgcgaccc accactccat gacgtcgtag gcgagccgcc
      841 ccgcctgcac cgacggatcg ccatcgctga gtcgtgccgc ctcggccagg ccgcaagcga
      901 agatcgacat cccccggaag cgcatgtccg actgctcacc gagtggccca ttggcgacga
      961 gcagcccctg ccgcttcagc tcggcacgat aggcaaggtg ccgcgcctgg agcgcgtcga
     1021 gctcctcgtc cgacatctgc ggcgcatccg ccggccgccg caacaggacg agcgtgaaca
     1081 cctcgaactc gtcggggatg ttgggatcgc gggccatggt gaccgcaggg taccgccgcc
     1141 catcgacggg ttggtgtcac cgaatgccct tcgccgtcgc catagttgtt gacggcggcg
     1201 ggtcgagtcg ccacgaaccg agggatgtgt ggatcaacga gggctcgtgg accgggcggc
     1261 ggcgggcgat cgagaggcgt tcggcgcact cgtgcgcgcg tctgccgctc ggctggacgg
     1321 cgcggctcgg ttgatcctgc gcgaccccga tcttgcgcga gatgccgtgc aggaggcgtt
     1381 gatccgcgcc tggcgcgacc tcccgacgct gcgcgaccgg gaccggttcg atgcgtggct
     1441 gcatcgactg ctggtgaacg ccgcaatcga tgaagcgcgc cgccggcgcc ggcgggtcgt
     1501 cgagatcgac cttgactccg tagcctcccc gagtactgcc gataccacca gccgccaggc
     1561 ggacagcgac gccctggatc gcgtcctgca gctcctcccg cccgagcatc gggcgctgat
     1621 cgtccagcac tactacctcg gggttccgct gccggaggcg gccaggatcc tcgggatctc
     1681 ccacgacgcc gcaaagcagc ggcttcaccg agcgatcgtc gcgctccgcc agaagttcga
     1741 gtcgcaggcc gacaacgcct ccatcagcct cgagaagggc aacgcatgag cactgcagac
     1801 cgcttcgacc gacacctgga gagcctcctc gccgagctca ccggtccgca gatgccggcc
     1861 tacatcgacg acgcgctggc gaccgcaacc gcgcgcccac agcgcgggcc gtcgatcctg
     1921 tcccgcttcg gagcgggaac ggccgggccg cggtaccgca tggcgctcgt gggcctgccg
     1981 gtcgtgctcg tcctggccgc ggcaatcgtc gggctgctcg tcatcggcaa ccagaacccg
     2041 acgccgagcc cgacaccgaa cgcgccaagc gccgggccga ccgagacgtc ggaggcctca
     2101 cctgtgccca caaccaagcc gacccccacg cctgcgccct ccctcgggac ctgcgacggc
     2161 tacgcgcgcg cggccgacgc gagggtcgtc gacgcagtgg gcgagcagcc gtgggagacg
     2221 gagaaggtcg tctcgccgcc ggcattctcg agcctcggtg atggcctcat cgccgggttc
     2281 gccgagcagc tcgcaagcgg gcctgccgtc gtcctcatcg atcccgcgga tgggaccgcg
     2341 tgccggctcg tcgatctcga gagcgccgcg gacctgtggg agctggagtg gagcccgctc
     2401 ggcgacgcgc tcgcgattgc cgtcggcaaa caggtgctcg tgtggtcggc agcgggtctc
     2461 acccaggcgc tgcgcctcgc cgactccggg ttcggcgtga ccctcgcctg gtcgccgacc
     2521 ggggaagcaa tcgccatcgg gagcggctcg ccgatcgccg tcgtgttcgc ggatggttcc
     2581 aggagaagcg ttgcaatcca acacgcgaca tcccttgcct ggtcgccgga tgcgagccgg
     2641 ttcgcggcca ttggagtgga taccgacgac ccggaggaca cgcgccgcct cgtcgtgatc
     2701 tcgggcgatg ggttggtgat cgatccgatc gacgagcggg ccgggctcgc gggctggctc
     2761 gacgggcaga cgatcctggc cgggtcctcg acggacgccg gtgcgctcga agcgttcgac
     2821 cttggtccac ggacctggcg cacgtggtcg cccgcctcga ccgagccgat cgggctcacg
     2881 agcatcatcg gctacgcgcc cgggctgacc ggactggcgt tcttcgcgaa cgaggacatc
     2941 aacaagttgg ccgcgccgat ggacctgatc gtgcgcgacc tcccatccgg cgagcggcac
     3001 gtgctcgcaa cgaaggccta cccgccgtcc aacgcgttcg gcaccggagc ctggtccccg
     3061 gacggcacgc acatcgcggt ctcggtcatt ggcgacccga ccgatcccag ggcgcgcccc
     3121 ggcgtctcga tctacgcgct cgccggcggg gacccgacct tcgtctcgcc gatccaggtc
     3181 atcctcaccg agggctcgtg gcagcccgtg ccgtaggtga tcgcgggtag gctcccggga
     3241 tggcgaagta cctggtcctc ctccgcggga tcaacgtcgg cggcaggaac aaggtcgcaa
     3301 tggcagcgct gcgaacggcc ctcgaaaggc tggggttcga gaacgtctcg acctacattg
     3361 ccagcggcaa cgtcttcctc gactccgacc tcgccgcgtc gaaggtcgcc gcgaagatcg
     3421 aggccgcgct cccgaaggcg ttcaagctcg acgccgagct catcagggtc gccgtcctct
     3481 ccgcggctga cctcgaagcc gtcgtgaaac gccgaccgaa gggcttcggc gccgagccgg
     3541 cgaagtacca cagcgacgcc gttttcctga tgggcatcga tgccgccgag gcaatgagcg
     3601 tgttcagccc gcgcgacggc gtggacacgg tctggccggg caagggcgtc atctactcgc
     3661 agcgcctgag tgcggagcgg acccggagcc gcctcaaccg gatcgtcggg acgcccgcct
     3721 atcgatcga
//
LOCUS       DATBSW010000216         7444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_514333, whole genome shotgun sequence.
ACCESSION   DATBSW010000216 DATBSW010000000
VERSION     DATBSW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514333"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /gene="metK"
                     /locus_tag="VIF63_06050"
     CDS             <1..1159
                     /gene="metK"
                     /locus_tag="VIF63_06050"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8238976.1"
                     /translation="KLCDQVSDAILDAIIREDPQARVACETATTTGLVVVLGEITTKT
                     YVDFQQVVRETVRQIGYTRSDYGFDAETCGTLISIKEQSPDIKQGVDAALEVRSGDVD
                     LAELGAGDQGMMFGFACRETPELMPLPIALAHRMARRLAEARKSGQLPYLRPDGKTQV
                     TVEYAHGVPQRVRTVVVAAQHDPDVDMDRLRDDLAEAVILSTIPRELRESDPVMHVNP
                     TGRFVVGGPMGDAGLTGRKIIVDSYGGMARHGGGCFSGKDPTKVDRSGAYAARWVAKN
                     VVAAGLADRFEIEVAYAIGIARPLSVSIETYGTGRVPDATILELINRHFDLRPQSIIS
                     ALDLRRPIYRQTAAYGHFGRLDLDLPWERTDKAELLASEVGIRMPEAVPAA"
     gene            1220..2299
                     /locus_tag="VIF63_06055"
     CDS             1220..2299
                     /locus_tag="VIF63_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8238977.1"
                     /translation="MYAVVMAGGGGTRLWPLSRPETPKPFLPLLDSRSLLQRTVDRIV
                     GHPELGLHPDDVAVVTERRYGPMVRQQLPGVRVIAEPLGRNTAAAIALATLQFERDPN
                     EVMLVLPADAWIDPERDGVYREVLKAMARVARDGGFGVEAPLVTLGIRTERPATEYGY
                     LIPSYDEGAVVDDIKMYPLSGFEEKPNLERAGQLYAQLGVAWNAGIFLWQRRAIRDAI
                     DRYTGLITMIGSVAASESGLQAAYDRLKPMSIDHAVMEGAASDHRVLMGSMAVGWSDL
                     GTWTQLIAALGGTGVGRVITPNEPAKATEADLVVERVGGRLAVSAGPRDILAQSPVAL
                     LAGAAAAPEPVHDLVNRVAEWEERQ"
     gene            2296..3759
                     /locus_tag="VIF63_06060"
     CDS             2296..3759
                     /locus_tag="VIF63_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238978.1"
                     /translation="MTETAPKSPIVFGTDGWRARIAEDFTFENVRRCADGVARYVAGR
                     GEQAKGVVIAYDRRFASEHFAMATAEVILAHDIPVAFAAHAVPTQMSSYEVVERSAAA
                     GIVITASHNPWTDNGFKVKAPTGSAGGPDMLAVIEKSIAEGAPVERRPFADGEAAGLV
                     ERYDPFEGYERFVRRTVDLDALKAAKIDVLVEPMWGAGAGWLSRLLGGGRLRVNEIHL
                     ERNPYFGGVNPEPIRPNIDEALTKIVRDGHELGLLLDGDADRAGAADEQGNFIHQLEV
                     TGLLFYYLAEHRGLREPVVVSVNNTSMAARLGKHYGIEVIESPVGYKFIGPLMIEHGA
                     MMGAEESGGYGFGMHLPERDGIYANLLVLDLVLREMARGVKTVSQMVAQFHELAGPSF
                     YTRVDVHVERAVYAETKRRLLEGLIATPPTALAGQPVTRTQALDTKDGFKFFVDDGSW
                     LLIRTSGTEPLVRVYTEATSAAARDSLVGAGEKLVRG"
     gene            3770..4168
                     /locus_tag="VIF63_06065"
     CDS             3770..4168
                     /locus_tag="VIF63_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238979.1"
                     /translation="MNTNPVVPPAESSLPRRVDKPWGHEIIWAHTERYVGKLLVIETG
                     RRLSFQYHEAKDEWIHVLSGRLLLTLENDAGEVQDLEVGPGAGARVAIRRRHRYTAIE
                     TCTLIEVSTPELDDVVRLEDDFGREGTNAP"
     gene            4206..4880
                     /locus_tag="VIF63_06070"
     CDS             4206..4880
                     /locus_tag="VIF63_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238980.1"
                     /translation="MTPDIDEPIALRLGTAWAVAVVTLALAGLAVLAPIGVLAAGVIK
                     PDTACADEATYKSLKGDDKAELTVINNSDETVKVWWLNYDGKRVFYRDLAKHTSYVQA
                     TWLTHPWVVSSTAGDCYRFLVMTSLKQSVTVAPETPDPAATVVPESSTAASAPGGSPA
                     AVVPEASPAATSPSNRPTTGATSPDYTWLLIMGVLASVVAVLIAIAAGTGRLPGFPRG
                     GRGGQP"
     gene            4980..5243
                     /locus_tag="VIF63_06075"
     CDS             4980..5243
                     /locus_tag="VIF63_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238981.1"
                     /translation="MAKKATKKQPDLKAAAYAIPGMIAAGVSSAAAGVSSAIDAGVAT
                     AKDVINDLQDGTKRPVRKAASPRKRAPKATTKSSSAKRSSTKS"
     gene            5429..5707
                     /locus_tag="VIF63_06080"
     CDS             5429..5707
                     /locus_tag="VIF63_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238982.1"
                     /translation="MHSSLIGKVEKANRYARELDRISIDRLALTFRGDNDTHHVSLDA
                     GQWHCTCHYFESWGSCVHLLTLQKVFGVMLPDAAQVSIFTPVQTAATA"
     gene            complement(5770..6840)
                     /locus_tag="VIF63_06085"
     CDS             complement(5770..6840)
                     /locus_tag="VIF63_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238983.1"
                     /translation="MTTNPGTRRLVAVIALAATILGQAPATSAHDPDPLLGTGTPWAQ
                     NQLLNYQWASGQVPPAWLATAIDQGAADSNATRGSQAAIYARADTAPSKIAYGGAVPC
                     SSYGIACMNRSGVPDSFGVWFRPHGAVLDWGTLRWCQAQSSPTNGCYDARRVALDEFG
                     HVQLLGHHVNYADESDYLDAVVQFAGRSRPKEGWNTHVFGTCDVARLQLEYELIRATD
                     PVSTCLSLATSLTVATDYPNIVSGYAAEITGELEIAAVTAARRLSGDPLSRRSITIQR
                     RYLGSTTWAVIGTMFARATDGAYVITVVPQATADYRLNFDAPSSEGLRDSTSGIVRIT
                     VSSSGSCVSTPNAKEQSIIPPC"
     gene            complement(6892..>7444)
                     /locus_tag="VIF63_06090"
     CDS             complement(6892..>7444)
                     /locus_tag="VIF63_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238984.1"
                     /translation="LGPLAPRVDLGPIWVTELRLDAKGRTLAVQSCAEPACVTRIVAL
                     DRPQAAPISIEGPDQGSIVGLATGRLFTWEHCPGMPCAIQAWDLGAGKHEALVQRAVG
                     TALTPDGRYLVVVLDPTGHAQRIDLATRVGQRIRGIAPGELPLGTGVGAYAGTEVGSN
                     ELALAAPGSDARAFDPAGAAVEP"
BASE COUNT         1152 a         2616 c         2619 g         1057 t
ORIGIN      
        1 caagctctgc gaccaggtca gcgacgccat cctcgacgcg atcatccgcg aggatcccca
       61 ggcgcgcgtc gcctgcgaga cggcgaccac gacggggctc gtcgtcgtcc tcggcgagat
      121 cacgacgaag acctacgtcg acttccagca ggtcgtgcgg gagaccgttc gccagatcgg
      181 ctatacgcgc tccgactacg gcttcgacgc agagacgtgc ggcaccctga tctcgatcaa
      241 ggagcagtcg cccgacatca agcagggcgt cgacgcggcc ctcgaggtcc gcagtggcga
      301 cgtcgacctc gccgagctcg gcgcgggcga ccaggggatg atgttcggct tcgcctgccg
      361 cgagacgccc gagctgatgc cgctgccgat cgcgctggcc catcgcatgg cccgccgtct
      421 cgcggaggcg cgcaagtccg gccagctgcc gtacctccgc cccgacggca agacccaggt
      481 caccgtcgag tacgcccacg gcgtgccaca gcgcgtgcgc acggtcgtcg tcgcggcgca
      541 gcacgacccc gacgtcgaca tggaccgcct ccgggacgac ctcgccgagg ccgtcatcct
      601 gtcgacgatc ccgcgcgagc tgcgcgagtc ggaccccgtg atgcacgtca atccgaccgg
      661 ccggttcgtc gtcggcgggc cgatgggtga cgcgggcctg accggccgca agatcatcgt
      721 cgactcgtac ggcggcatgg cccgccacgg tggcggctgc ttctcgggca aggacccgac
      781 gaaggtcgac cggtccggcg cctacgccgc gcgctgggtc gcgaagaacg tcgtcgccgc
      841 ggggctcgcc gaccgattcg agatcgaggt cgcgtacgcg atcgggatcg cgcgaccgct
      901 gtcagtgtcc atcgagacgt acgggacggg ccgcgtgccg gacgcgacga tcctcgagct
      961 catcaaccgc cacttcgacc tgcgcccgca gtcgatcatc tccgcgctgg acctgcgccg
     1021 cccgatctac cggcagaccg ccgcgtacgg ccatttcggc cggctcgacc tcgatctgcc
     1081 ctgggagcgg accgacaagg ccgagctcct ggccagcgaa gtcggcatcc gcatgccgga
     1141 ggccgtcccg gcggcgtagt cggactcaca ttcgcggccc cgagcccacg gcgcggcccg
     1201 cgcgaatagc attcccctga tgtacgcagt ggtgatggcc ggcgggggtg gcacgcgact
     1261 ctggccgctg agccggcccg agacgcccaa gccgttcctg ccgctcctcg actcgcgatc
     1321 gctcctccag cggaccgtcg accggatcgt cgggcacccg gagctcggcc tgcatcccga
     1381 cgatgtcgcc gtggtcacgg agcgtcgcta cgggccgatg gttcggcagc agctgcccgg
     1441 ggtccgggtg atcgccgagc cgctcgggcg caacacggcc gccgcgatcg ccctcgccac
     1501 gctccagttc gagcgcgatc cgaacgaggt catgctcgtg ctgccggccg atgcctggat
     1561 cgaccccgag cgcgacggcg tctaccgcga ggtcctcaag gcgatggccc gggtcgcgcg
     1621 cgacggcgga ttcggggtgg aggcgccgct cgtcacgctc ggcatccgga cggagcgacc
     1681 cgccacggag tacggctacc tgatcccgag ctatgacgag ggcgccgtcg tcgacgacat
     1741 caagatgtac ccgctgtcgg ggttcgagga gaagccgaat ctcgagcggg cgggccagct
     1801 gtacgcgcag ctcggcgtgg cctggaacgc gggcatcttc ctgtggcagc gccgcgcgat
     1861 ccgtgacgcg atcgatcggt acaccggctt gatcacgatg atcggcagcg tggccgcgag
     1921 cgagtcgggc ctccaggcgg cgtacgaccg gctgaagccg atgtcgatcg accatgcggt
     1981 catggagggc gccgcgagcg accaccgcgt gttgatgggc tcgatggccg tcggttggag
     2041 cgatctcggc acctggacgc agctcatcgc ggcgctcggc ggcacgggcg tgggccgggt
     2101 gatcacgccg aacgagccgg ccaaggcgac agaggcggat ctcgtcgtcg agcgcgtcgg
     2161 cggacgcctc gccgtgagcg ccggtccgcg cgatatcctc gcccagagcc ccgtggcgct
     2221 cctcgccgga gccgcagcag cccccgagcc ggtgcacgac ctcgtgaacc gcgtcgccga
     2281 atgggaggaa cgccagtgac cgagacggcg ccgaagtccc cgatcgtctt cggcaccgac
     2341 ggctggcggg cgcgaatcgc cgaggacttc acgttcgaga acgtccggcg ctgcgccgac
     2401 ggggtggctc gctacgtcgc cggcaggggc gagcaggcga agggcgtcgt catcgcctat
     2461 gaccggcgct tcgcctcgga gcacttcgcg atggcgacgg ccgaggtgat cctcgcccac
     2521 gacatcccgg tcgcgttcgc cgcccacgcc gtgccgaccc agatgagctc gtacgaggtc
     2581 gtcgagcgga gcgcggcggc gggcatcgtg atcaccgcca gccacaaccc ctggaccgac
     2641 aacgggttca aggtcaaggc tccgacggga tccgctggcg gcccggacat gctcgcggtc
     2701 atcgagaagt cgatcgccga gggcgcgccg gtcgagcgcc ggcccttcgc cgacggcgag
     2761 gccgccggcc tcgtcgagcg ctatgacccg ttcgagggct acgagcgatt cgtccgtcga
     2821 accgtcgacc tcgatgcgct caaggcggcc aagatcgatg tcctggtcga gccgatgtgg
     2881 ggcgcgggcg cgggctggct ctcgaggctg ctcggcggcg gccgcctccg cgtcaacgag
     2941 atccacctcg agcgcaaccc gtacttcggc ggcgtgaacc ccgagccgat ccggccgaac
     3001 atcgacgagg ccctgacgaa gatcgtccgc gacggccacg agctcgggct gctgctcgac
     3061 ggcgacgcgg accgggccgg cgctgccgac gagcagggca acttcatcca ccagctcgag
     3121 gtcaccggcc tgctcttcta ctacctggcc gagcaccgcg ggctgcgcga gccggtcgtc
     3181 gtgtcggtga ataacacgtc gatggccgca cgcctcggca agcactacgg catcgaggtc
     3241 atcgagagcc cggtgggcta caagttcatc ggcccgctga tgatcgagca cggcgcgatg
     3301 atgggcgccg aggagtccgg cgggtacggc ttcggcatgc acctgccaga gcgcgacggc
     3361 atctacgcca atctgctcgt gctcgacctc gtcctgcgcg agatggcccg gggcgtgaag
     3421 acggtctccc agatggtcgc ccagttccac gagctcgccg gaccgtcgtt ctacacgcgc
     3481 gtcgacgtcc acgtcgagcg agctgtctac gccgagacga agcggcgcct gctcgagggc
     3541 ctcatcgcga caccgccgac ggcgctcgcg ggccagccgg tgacgcgaac ccaggcgctc
     3601 gacacgaagg atggcttcaa gttcttcgtc gacgacggct cgtggctgtt gatccgaacg
     3661 tcgggcaccg agccgctcgt gcgcgtgtac acggaggcga cgtccgcggc cgcgcgcgat
     3721 tcgctcgtcg gagcgggcga gaagctcgtc cgcgggtgag cggagcatcg tgaacacgaa
     3781 cccggtcgta ccgccggccg agtcgtcgct gccgcggcgc gtcgacaagc cctggggcca
     3841 cgagatcatc tgggcccaca cggagcgcta cgtcggcaag ctgctcgtca tcgagaccgg
     3901 ccggcgcctg tccttccagt accacgaggc gaaggacgag tggatccacg tcctgtcggg
     3961 ccggcttctg ctcaccctcg aaaacgacgc cggcgaggtg caggacctcg aggtcggtcc
     4021 cggcgctggc gcccgggtcg cgatccgccg gcgccaccgc tacacggcga tcgagacctg
     4081 cacactgatc gaggtctcga cccccgagct cgacgacgtg gtccgcctcg aggacgactt
     4141 cggccgcgag ggcacgaacg ctccataggc catccgccgc cacagccggg cgatatcctg
     4201 cggccgtgac accggacatc gatgaaccga tcgcgctacg ccttggaacg gcttgggccg
     4261 ttgccgtcgt caccctcgcc ctcgctgggc tcgctgtgct cgcaccgatc ggcgtcctcg
     4321 cagccggcgt catcaagccc gacaccgcct gcgccgacga ggccacctac aagtcgctca
     4381 agggcgacga caaggccgag ctgaccgtga tcaacaacag cgacgagacc gtcaaggtct
     4441 ggtggctgaa ctacgacggc aagcgagtgt tctatcgcga cctcgccaag cacaccagct
     4501 acgtccaggc gacctggctc acgcatccgt gggtcgtctc gagcacggcc ggcgactgct
     4561 accggttcct cgtcatgacc tcgctcaagc agagcgtgac cgtggcgccc gagacccccg
     4621 accccgcggc tactgtcgtc ccggagtcct cgaccgcggc atcggcgccg ggcgggtcgc
     4681 ctgcggcagt cgtccccgaa gcgagccctg cggcgacgtc cccgtcgaac cgaccaacca
     4741 ccggcgcgac gtcgcccgac tacacctggc tcctgatcat gggcgtgctg gcctcggtcg
     4801 tggcggtgct gattgccatc gctgcaggta cgggtcgtct ccccggattc ccgcgcgggg
     4861 gacgcggcgg ccagccttag gcaccgcaat gcacatgcag gtcgcacggg ctcgcgacat
     4921 gcattggcgc aaccggaagt cgacgagcat cgagaccttc atcgcaggga gggctcgtca
     4981 tggccaagaa ggcaacgaag aagcaacccg atctcaaggc cgccgcctac gcgatcccgg
     5041 ggatgatcgc cgccggcgta tcgagcgccg ccgccggcgt gtcgagcgcg atcgacgcgg
     5101 gcgtcgcgac cgcgaaagac gtcatcaacg acctccagga cggcaccaag cgcccggtcc
     5161 gcaaggcggc aagcccgcga aagcgcgcgc cgaaggcgac gacgaagagc tccagcgcga
     5221 agcgttcgag cacgaagagc taggcctgcc ctctcccggc gagggggatc gcccgcgagt
     5281 cccgcgggag cgcggccagc gtcaggcaga ggggtgactg ggtcccgcgg acgggtgtag
     5341 gcttgacccg cgcccgcctc gcctggtcgg tgcaggaccg gacggcggcc gccattgccc
     5401 cgacgcgggg cctggagggt ctggccagat gcacagctcc ctgatcggca aggtcgagaa
     5461 ggcgaatcgt tacgcccgcg agctcgatcg aatttccatc gatcgtctcg ccctcacgtt
     5521 ccgcggtgac aacgacaccc accacgtgag cctcgatgcc ggccagtggc actgcacttg
     5581 ccactatttc gaatcgtggg gtagttgcgt ccaccttctc accctccaga aggtgttcgg
     5641 cgtgatgttg cccgacgcgg cccaggtctc gatcttcacg cccgtccaga ccgccgcgac
     5701 ggcgtgaatc ggggaggccg ggcgagcgcc cgacctccca ccccgaaccc agggaccggc
     5761 ccgcgcccgt cagcacggcg ggatgatcga ctgctctttc gcgttcggcg tggacacgca
     5821 tgatccgctg ctgctgaccg tgatccggac gatgcccgag gtcgagtctc gcagcccttc
     5881 gctcgatggc gcgtcgaagt tgagccggta gtcggccgtc gcctggggca cgaccgtgat
     5941 gacgtaggcg ccgtcggttg cccgggcgaa catcgtgccg atgactgccc acgtcgtcga
     6001 accgaggtac cgccgctgga tcgtgatgct gcgccggctc agcggatccc cggagaggcg
     6061 gcgtgccgcg gtgacggctg caatctcgag ctcaccggtg atctccgccg cgtagcccga
     6121 gacgatgttc gggtagtcgg tggccaccgt cagggacgtg gcgaggctca ggcaagtcga
     6181 gaccgggtcg gtggcgcgga tgagctcgta ctcgagctgg aggcgggcaa cgtcgcaggt
     6241 cccgaagacg tgggtgttcc agccctcctt gggccgcgac cggccggcga actgcacgac
     6301 ggcgtcgagg tagtcgctct cgtccgcgta gttcacgtgg tgcccgagga gctggacgtg
     6361 tccgaactcg tcgagtgcga cccgacgcgc gtcgtagcaa ccgttggtgg ggctcgactg
     6421 cgcctggcac cagcgcaacg ttccccagtc gagcaccgcg ccgtgcggcc ggaaccagac
     6481 cccgaagcta tccgggacgc cgctccggtt catgcacgcg atgccgtagc tcgagcaggg
     6541 gacggcgccg ccgtacgcga tcttgcttgg ggccgtgtcg gcgcgggcgt agatcgccgc
     6601 ctgcgagccc cgggtcgcgt tcgaatcggc ggcaccctgg tcgatcgcgg tcgcgagcca
     6661 ggcgggtggg acctggcccg atgcccactg gtagttcagg agctggttct gcgcccaggg
     6721 cgtgccggtg ccgaggagcg ggtcggggtc gtgcgcggac gtcgcgggcg cctggccgag
     6781 gatcgtggcg gcgagcgcga tgacggcaac gagccgtcga gtgccggggt tcgtggtcat
     6841 gaagatggtc ctccgcggcg gctgggatcg aggcagcggg ggagcgcggc gtcagggttc
     6901 gacggcggct cccgccggat cgaacgcgcg ggcgtcgcta ccgggtgcgg cgagggcaag
     6961 ctcgttcgag ccgacctcgg tcccggcgta cgcaccgacg ccggtgccga gtggcagctc
     7021 gcccggagcg atgccccgaa tccgctggcc gacccttgtc gcaaggtcga tccgctgggc
     7081 atgcccggtc ggatccagga ccacgacgag gtagcggccg tcgggcgtca gggccgtgcc
     7141 aaccgcccgc tggacgagcg cctcgtgctt cccggcgccc aagtcccatg cctgtatcgc
     7201 gcagggcatg cccgggcagt gctcccaggt gaagaggcga ccggtggcga gcccgacgat
     7261 cgagccctga tccggaccct cgatgctgat gggggcagcc tgcggccggt ccaacgcgac
     7321 gatgcgcgtc acgcacgcgg gctcggcgca cgactggacc gcgagcgtgc ggcccttggc
     7381 gtcgaggcgg agctcggtga cccagatggg gccgaggtcg acgcgcggtg cgagcggtcc
     7441 gagg
//
LOCUS       DATBSW010000217         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1219466, whole genome shotgun sequence.
ACCESSION   DATBSW010000217 DATBSW010000000
VERSION     DATBSW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219466"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VIF63_06095"
     CDS             complement(<1..721)
                     /locus_tag="VIF63_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8238985.1"
                     /translation="MRVLLALDGSPASDAARRLVASLPWPEGTVLHVVGAVEPVVDSF
                     AALSGYPGLAADTEPAFESTLSAILDEACTSLEAAGRIVRRSVHVGRAGSVIVDEASA
                     MRAELIVVGSRGLGPMKSMVLGSVSAEVVDHAPCPVLVVRRPQIAKILFATDGSASAR
                     GALTFLASLGCLAECPIEVISVGVRPPSLAALELGASRDAAERFSHESIVDREHVEAR
                     AASAVETLRASGRPARWSIAAG"
     gene            878..1354
                     /locus_tag="VIF63_06100"
     CDS             878..1354
                     /locus_tag="VIF63_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8238986.1"
                     /translation="MVAAPAADMLRSTWFAATLSVHVLERLTQLGTTAEYETGTAVIR
                     EGEPCEALGVLVDGRMSIRLRLPGGEARSILTIEPGDVFGWSAILAPSIATSTVTAVT
                     RTRTVVFEGARLLDAMTADQELAAAVHRRVLVAVARRLQATRVQLLDLYASSYDPW"
     gene            1351..>2446
                     /locus_tag="VIF63_06105"
     CDS             1351..>2446
                     /locus_tag="VIF63_06105"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8238987.1"
                     /translation="MTSTPVFLPRKELAHLFDALRADGRRIVGPTVVDGAVIYDELTS
                     PTELPTGIRADTGPGRYRLSGEGGLRAFDYTVGVTSWKRFNHPPIVPLTRSRRDGDRT
                     IVEAVAEDPPRLAYLGVRGCELAAIGIQDRAMRAGPLGDASHAERSDAALIVGVECAV
                     AASTCFCTSMGTGPGIEEGRGADVVLAELDDGFVVRAETAAGRRVVEQLELRPAADGQ
                     VQMAAQQVSTLGAAMGDPVPTTGLPERLRAALDHPRWDEVAERCLACANCTLVCPTCF
                     CTSVGVASDLDGQSAATERTWDSCFSLGFGRVAGDANFRPKTRDRYRQWLTHKFSTWW
                     DQFGSSGCVGCGRCIAWCPVGIDVREELLAI"
BASE COUNT          363 a          861 c          863 g          359 t
ORIGIN      
        1 ctcccgccgc gatcgaccat cgagccgggc ggccgctggc ccgcagcgtt tcgacggcgg
       61 acgccgctct cgcctcgacg tgctcgcgat cgacgatgga ttcgtgggag aaccgctccg
      121 ccgcatccct cgaagcgccg agctcaagag ccgcaaggct cggcgggcga acgccgacgc
      181 tgatgacctc gatcgggcat tccgcgaggc agcccaggct cgcgaggaag gtgagggcgc
      241 cccgtgcgga tgccgagccg tcggttgcaa agaggatctt ggcgatctgc ggtcgccgga
      301 cgacaaggac gggacatggg gcgtggtcga ccacttcggc ggagaccgag ccgaggacca
      361 tggacttcat cggcccaagc ccgcggctgc cgacgacgat cagctccgcg cgcatcgcgc
      421 tcgcttcgtc gacgatgacg ctgccggcgc ggccgacgtg gaccgatcga cgaacgatcc
      481 ggccggccgc ctcgagcgac gtgcaggcct cgtcgagaat cgcagacaac gtagattcga
      541 acgcaggctc cgtgtccgca gccagaccgg gatagcccga caacgcggcg aacgagtcga
      601 caacgggttc gacggcgccg acgacgtgga gcaccgtgcc ttcgggccac gggagtgaag
      661 cgacgagccg ccgcgccgca tccgacgctg gcgagccgtc gagggcaagc aggactcgca
      721 tctcagttcc tccttattga ctcatcggtc ggccgggcgc ggtcgatccg acgatcagca
      781 tgtggcgaat gggtcccggc ccgcgacggc catccgaccc gccgtcgcag gccgtatggc
      841 tctcgcgcga gtgcaggccg ccgccgagca ttgggtcatg gttgccgcgc cagccgccga
      901 catgctgcga tccacgtggt ttgccgccac gctgtccgta cacgtcctgg agcggctgac
      961 ccagctcggc acgacagcgg agtacgagac cgggacagcg gtcatccgcg agggcgagcc
     1021 gtgcgaggcc cttggcgtgc ttgtcgacgg gcgcatgtcg attcggcttc gccttccggg
     1081 tggcgaggcc cgctcgatcc tcacgatcga acctggcgac gtcttcggct ggtcggccat
     1141 cctcgccccg tcgatcgcga cgtcgaccgt aacggccgtc acccgcaccc gaaccgtcgt
     1201 gttcgaaggc gcgcggctgc ttgacgcgat gacggccgat caggagctcg ccgcggccgt
     1261 tcaccggcgg gtcctggttg ccgtcgcgcg gcgcctccag gcgacgcgcg ttcaactcct
     1321 cgacctgtat gcctcgagct acgacccgtg gtgacctcga cgcccgtctt cctgccgagg
     1381 aaggagctcg cccacctctt tgacgccttg cgcgcagacg gacgccgaat cgtcggaccg
     1441 accgtcgtcg acggcgccgt gatctacgac gagctgacaa gccccaccga gctcccgacc
     1501 gggatccgtg ccgacacggg tccgggccgc tatcggctct cgggcgaagg cggccttcgg
     1561 gccttcgact acacggtggg cgtcacgtcc tggaagcgct tcaaccatcc accgatcgtg
     1621 ccgctgacgc ggtcccgacg cgacggtgat cgaacgatcg tggaggccgt cgcagaggat
     1681 ccgccccggc tcgcctacct cggcgtccgc ggctgcgagc tggccgcgat cggaatccag
     1741 gaccgggcga tgcgcgccgg accgctcggt gacgcgtcgc atgccgagcg cagtgacgcc
     1801 gcactgatcg tcggggtcga atgcgccgtc gccgcgagca cgtgcttctg cacgtcgatg
     1861 ggcacgggcc cagggatcga agagggccgc ggtgccgatg tggtgctggc ggagctcgac
     1921 gacgggttcg tcgtgcgtgc cgagaccgcc gccggtcgac gcgtggtcga gcagctggag
     1981 ctgcgtcccg cggccgacgg ccaggtccag atggccgccc agcaggtgag cacgttgggg
     2041 gccgcgatgg gcgaccccgt gccgacaacc ggcctcccgg agcgcctgcg cgcggcgttg
     2101 gatcatccgc gctgggacga ggtcgccgag cgctgcctcg cgtgcgcgaa ctgcaccctc
     2161 gtctgcccga cgtgcttctg caccagcgtg ggcgtggcct ccgacctcga cggccagagc
     2221 gcggccaccg agcggacctg ggacagctgc ttcagtctcg gcttcgggcg cgtcgccggg
     2281 gacgcgaact tccggccgaa gacgcgcgac cggtaccggc agtggctgac ccacaagttc
     2341 tcgacgtggt gggatcagtt cggctcgagc ggctgcgtcg gttgcggccg ctgcatcgcc
     2401 tggtgcccgg tcgggatcga cgtccgcgaa gagctcctcg cgatcg
//
LOCUS       DATBSW010000218         6295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_761267, whole genome shotgun sequence.
ACCESSION   DATBSW010000218 DATBSW010000000
VERSION     DATBSW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761267"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1482
                     /gene="mtnA"
                     /locus_tag="VIF63_06110"
     CDS             67..1482
                     /gene="mtnA"
                     /locus_tag="VIF63_06110"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY8238988.1"
                     /translation="MTDKAPEPRPDNETAPDPTRRQFFRRFAGDVITSAAQVVGAVTE
                     IRDRSAAEAAVLFGEPSVSADAAAGLAPTGSAPAGEAAISTDGAPSGFRTPFRFESDN
                     VLLVIDQRKLPDALVEVPVRSASDAANAIRLMIVRGAPAIGQVAAIGLALTARITVES
                     QSHTRRAILEGAANTLRSARPTAINLRWAVDRLMARYHAIGQLSREGQQIADAMHDEA
                     MAIVAEATDDHGRLAEAGLAVLPVPADRPLRILTHCNTGPLACGQFGTALGIVQAAHQ
                     AERPLHVWVDETRPYLQGARLTAWELQQAGVPHTLIPDMAAGPLMARGEVDIILVGAD
                     RIAANGDTANKLGTYTLAVLAARHDVPFVVCAPLSSVDLATADGAAIPIEDRSADEVT
                     LIGGTRIAPEGTRVRNPSFDVTPAELISAIVTEEGVLRAPFGPALEDAMQQRNARRPP
                     APTPELSTAAGVAPDLAEVAG"
     gene            1482..2354
                     /locus_tag="VIF63_06115"
     CDS             1482..2354
                     /locus_tag="VIF63_06115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8238989.1"
                     /translation="MATVALDRAGTRLVAETTTDRALLRTFLERDRIYAAYALADLED
                     REVARSRWGIARAGDEVVSLVLEYGGPSPQPLFVTGRDDGVEAILRDVIKPSIAYVAC
                     LPSSARAVETRYRLEPGPQMVRMWVDRETFVPSFDPGVEALSANDAGELNRLYRLGFG
                     SWLPPQAVAEGIYRGIRVNGRLVAAAGTHVIGRNAAIAVVGNVLTQPEFRGRGYAQAT
                     TAAVTAHLLEFCDHVVLNVRSDNPPALNAYRRLGYAEHVRFEERLGHRIGSLWADIAS
                     ALRRFAQRRESQRR"
     gene            2351..3628
                     /gene="ahcY"
                     /locus_tag="VIF63_06120"
     CDS             2351..3628
                     /gene="ahcY"
                     /locus_tag="VIF63_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8238990.1"
                     /translation="MTMAHEATTHDVTNLALASEGVRRIEWAEREMPVLRSIRERFAL
                     ERPLDGLRVGACLHVTSETANLMRTLAAGGAQVVLAASNPLSTQDDVAAALVTEYGVP
                     VHARRGEDRDTYYRHLNSVADTHPTITMDDGCDLVSLLHQDRAEQVKEVLAGTEETTT
                     GVIRLRAMAADNALGYPVIAVNEAQTKHLFDNRYGTGQSTLDGILRATNLLIAGRNVV
                     VAGYGWVGRGIASRMAGHGGHVAIVEIDPVRALEALMDGYQVMTAGQAAGWGDLFVTA
                     TGNVNVFRREHFESMRDGAVMANSGHFDAELDLAALRQMAEGHIRDVRENVQEFDVGG
                     KRLHLIAEGRLVNLGAAEGHPAAVMDMSFANQALAAEYVARHHAELDNRVYGVPPAID
                     AEVARLKLLAFGVELDAMTAEQAEYVTAWKHGT"
     gene            3647..5608
                     /locus_tag="VIF63_06125"
     CDS             3647..5608
                     /locus_tag="VIF63_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8238991.1"
                     /translation="MTLASVTVAPFGSWASPFRIERLTDRVVFLTEARGVDGVRWWLE
                     GRPDEGGRQVLVRRDVDGTLTRLTPPGFNARSRVHEYGGAAALISGDLIVVSDFVTGR
                     LNRVVAPEELVPLTPEKAWRYSDVIHDVERNRLIAVREDHEEAMVASHGEWNNDLVAI
                     DLETGAVSVLAAGSDFYFAPRLSPDGTKLAWLEWHHPNMPWDGTELMLAPVAADGSLG
                     EAQVIAGSRRDWISQPRWSPDGVLHFAAEPHGWMNLYRYVDGEAEAITDYEAEFVPPD
                     WQFGYTTYEFLGDGSILAIARSGGRDQLVRVAHDGTLSPIDIPYTEMAGLSIDGERVI
                     MRAAGPARPASVIDLDLDGNVTILRTATPNIPEREDTSISEHIEFPTTGNRMAYGNYY
                     PPTNRSFVGPDGELPPLIVTSHGGPTAAAFSGFATGLQLFTSRGYAVLDVDYGGSTGY
                     GRDYRKRLEGQWGIVDVDDCVAGAKYLTEQGLVDGERLAIRGGSASGFTTLAALAFTD
                     QFDAGCTYFGIGDLRAFVKDTHKFESRYLESLLGPWPEAKQVYLDRSPSLHAEKITAP
                     VLVQQGAEDKIVPQAEAERIVDALFERRVPHAYLLYPGEDHGFRGHDAIIRSFGAELS
                     FYAQVFGFETADDIEPLEIQFLEESRRST"
     gene            complement(5983..>6295)
                     /locus_tag="VIF63_06130"
     CDS             complement(5983..>6295)
                     /locus_tag="VIF63_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8238992.1"
                     /translation="LHNKYLDIKRSAAYDVPSTREGERQVVAMMWTNKGFEIWMGDAG
                     SDLSRSVGTAASIGAQAFEGTPFASDPCTDAMVARKAGWLSRAISAITDRAQLPRIAR
                     G"
BASE COUNT          950 a         2287 c         2133 g          925 t
ORIGIN      
        1 cgcgtgacga gcgtcatccg gtattgatcg acccgatcac ggtcgagcct cgctgaacgt
       61 gcaccgatga ccgacaaggc gcccgagcca cgaccggaca acgagacggc acctgacccg
      121 acacgccgcc agttcttccg caggttcgcc ggcgacgtga tcaccagcgc cgcacaagtc
      181 gtcggggcgg tcaccgagat ccgcgatcga tccgccgccg aggccgctgt cctgttcggc
      241 gagcccagtg tctcggcaga tgcagccgcc ggcctggcgc cgacgggttc ggcaccggca
      301 ggggaggcgg ccatttcgac cgatggcgcc ccgagcggct tccggacccc gttccgcttc
      361 gagtccgaca acgtcctcct cgtcatcgac cagcgcaagc tgcccgacgc cctcgtcgaa
      421 gtgcccgttc gcagcgcgtc ggacgccgcg aatgcgatcc ggctgatgat cgtccgcggt
      481 gcgccggcga tcggccaggt ggcggccatc gggctcgcgc tcaccgcgcg gatcaccgtc
      541 gagtcgcagt cgcacacgcg gcgggcgatc ctcgagggcg ccgcgaacac actgcgaagc
      601 gcccgcccga ccgcgatcaa cctgcgctgg gcggtcgatc gcctgatggc ccgctaccac
      661 gcgatcggcc agctctcccg cgaaggccaa cagatcgccg acgccatgca cgacgaggcg
      721 atggccatcg tggccgaggc gaccgacgac catggccggc tcgccgaggc gggtctggcc
      781 gtcttgccgg tgccggccga ccggccgctc cggatcctga cccactgcaa caccggcccg
      841 ctggcctgcg gccagttcgg tacggcgctc ggcatcgtgc aggccgctca ccaggcggag
      901 cggccgctgc acgtgtgggt ggacgagacc cgcccctatc tccagggcgc ccgcctcacc
      961 gcttgggagc tgcagcaggc cggcgtgccc cacacgctca tccccgacat ggccgccggc
     1021 ccgctcatgg cccgcggcga ggtcgacatc atcctcgtcg gcgcagatcg gatcgcggcg
     1081 aacggcgaca ccgcgaacaa gctcgggacg tacacgctgg ccgtgctcgc ggcgcgccac
     1141 gacgtgccgt tcgtcgtctg cgccccgctg tcgtcggtgg acctcgcgac ggccgacggc
     1201 gccgccatcc cgatcgagga tcggtcggcc gacgaggtca cgctcattgg cggcacccgg
     1261 atcgcgcccg agggcacccg ggtccgcaac ccctcgttcg atgtcacgcc ggcggagctg
     1321 atcagcgcga tcgtcaccga ggagggcgtg ctgcgggcgc cgttcggacc ggcgctcgaa
     1381 gatgcgatgc agcagcggaa tgcgcgccgc cctccagcgc cgacccccga gctgtcaact
     1441 gccgccggcg tcgcgcctga cctggccgag gttgccggct gatggccacc gtcgccctcg
     1501 atcgggccgg gacgcggctg gtcgccgaga ccacgacgga tcgcgcgctc ctgcgcacct
     1561 tcctcgaacg cgaccgcatc tacgccgcct atgccctggc cgatctcgag gatcgcgagg
     1621 tcgcccggtc ccgctggggg attgcccgcg ccggtgatga ggtcgtgtcg ctcgtcctcg
     1681 aatacggcgg tccctcgccc cagccgctgt tcgtcacggg ccgtgacgac ggggtcgagg
     1741 ccatcctgcg cgacgtcatc aagccgtcga tcgcgtacgt cgcgtgcctg ccctcgagcg
     1801 cccgtgcggt cgagacgcgc taccgcctcg aacccggccc gcagatggtc cgcatgtggg
     1861 tcgaccggga gaccttcgtg ccctcgttcg accccggcgt cgaggcgctc tccgcgaacg
     1921 atgccggcga gctcaaccgc ctgtaccggc tcggcttcgg atcgtggctg ccgccgcagg
     1981 ccgtcgccga gggcatctat cgagggatcc gggtgaacgg ccggctcgtc gctgcggcgg
     2041 gcacgcacgt catcggccga aacgcggcca tcgccgtggt cggcaacgtc ctgacccagc
     2101 cggagttccg cggccgcggc tacgcgcagg cgacgaccgc cgcggtcacc gcccacctgc
     2161 tcgagttctg cgaccatgtc gtcctcaacg ttcgctcgga caatccgccg gcgctcaatg
     2221 cgtaccggcg ccttggctac gccgagcacg tacggttcga ggagcggctc ggacaccgga
     2281 tcggctcgct gtgggccgac atcgcgtcgg cgctccgacg gttcgcgcaa cgcagggagt
     2341 cccagcgacg atgaccatgg cccacgaggc gacgacccac gacgtcacga atctcgcgct
     2401 ggcgagcgag ggtgttcgac gcatcgagtg ggccgagcgc gagatgcccg tcctgcgctc
     2461 gatccgcgag cgattcgcgc tggagcggcc gctcgacggc ctgcgcgtcg gggcgtgcct
     2521 ccacgtcacc tccgagacgg ccaacctgat gcgcaccctg gctgccggcg gcgcccaggt
     2581 cgtgctcgcg gcctcgaacc cgctgtccac gcaggacgac gtcgcggcgg cgctcgtgac
     2641 ggaatacggg gtccccgtcc acgcccggcg cggcgaggat cgcgacacct actaccggca
     2701 cctgaactcg gtcgcggaca cccacccgac gatcacgatg gacgacggct gcgacctcgt
     2761 ctcactgctc catcaggacc gggccgagca ggtcaaggag gtcctcgcgg ggaccgagga
     2821 gacgacgacg ggcgtgatcc gactgcgcgc gatggccgcg gacaacgcgc tcggctaccc
     2881 ggtcatcgcg gtcaacgagg cccagacgaa gcacctcttc gacaaccgct acggcaccgg
     2941 ccagtcgacg ctcgacggca tcctgcgcgc gaccaacctg ctgatcgctg ggcgcaacgt
     3001 cgtcgtggcg ggctacggct gggtggggcg ggggatcgcg tcgcggatgg ccggccacgg
     3061 cgggcacgtc gcgatcgtcg agatcgaccc ggtgcgcgcg ctcgaggcgc tcatggacgg
     3121 ctaccaggtc atgaccgccg gccaggcggc cggctggggc gacctgttcg tgaccgccac
     3181 cggcaacgtc aacgtcttcc gccgcgagca cttcgagtcg atgcgcgacg gcgccgtcat
     3241 ggccaactcc ggccacttcg acgccgagct ggacctcgcc gcgctgcgcc agatggccga
     3301 gggccatatc cgcgacgtcc gcgagaacgt gcaggagttc gacgtcggcg gcaagcgact
     3361 gcacctgatc gccgagggcc ggctcgtgaa cctcggcgcg gccgagggcc acccggcggc
     3421 cgtgatggac atgagcttcg ccaaccaggc gctggccgcc gaatacgtcg cgcgccacca
     3481 cgccgagctc gacaaccgtg tgtacggcgt gccaccggca atcgatgccg aggtcgcccg
     3541 gctcaagctc ctcgccttcg gcgtcgagct cgacgcgatg accgccgaac aggccgagta
     3601 cgtcacggcc tggaagcacg gcacctagca ggcacggaga ggttcgatga cactcgcatc
     3661 ggtcacggtc gcgcccttcg ggtcgtgggc gtcgccgttc aggatcgagc ggctgacgga
     3721 ccgggtcgtg ttcctcaccg aggcccgcgg cgtcgacggc gtccgctggt ggctcgaggg
     3781 ccggccggac gagggcggcc gccaggtgct ggtccggcgc gacgtcgacg gcacgctgac
     3841 ccggctcacg ccgcccggct tcaatgcgcg ctcgcgggtc cacgaatacg gcggcgcggc
     3901 ggcgctgatc tcgggcgacc tgatcgtggt gtcggacttc gtgaccggcc gcctgaaccg
     3961 tgtcgtcgca cccgaggagc tcgttcccct cacccccgag aaggcgtggc gctactccga
     4021 cgtgatccac gacgtcgagc gcaaccggct catcgcggtc cgcgaggacc acgaggaggc
     4081 catggtcgcc agtcacggcg agtggaacaa cgacctcgtc gcgatcgatc tcgagaccgg
     4141 cgcggtctcc gtgctcgcgg cgggctcgga cttctacttc gcgcctcgac tctcgcccga
     4201 cggcacgaag ctcgcctggc tcgagtggca ccacccgaac atgccctggg acgggaccga
     4261 gctcatgctc gctcccgtgg cggccgatgg cagcctcggc gaggcgcagg tcatcgccgg
     4321 cagccggcgc gactggatca gccagccccg ctggtcaccg gacggcgtcc tccacttcgc
     4381 cgccgagccc catggctgga tgaacctgta ccgctacgtc gacggcgagg ccgaggcgat
     4441 caccgactac gaggcggagt tcgtcccgcc cgactggcag ttcgggtaca cgacgtacga
     4501 gttcctcggc gacggctcga tcctcgcgat cgcccgctcg gggggccgcg accagctggt
     4561 tcgcgtcgcc cacgatggca ccctctcgcc gatcgacatc ccgtacaccg agatggccgg
     4621 gctctcgatc gatggcgaac gcgtgatcat gcgggccgcc ggcccggccc ggccggcctc
     4681 ggtcatcgac ctggacctgg atggcaatgt gacgatcctt cggacggcga ccccgaacat
     4741 ccccgagcgc gaggacacct cgatctccga gcacatcgag ttcccgacga cgggcaatcg
     4801 gatggcctac ggcaactact acccgccgac caaccgctcc ttcgtcggtc ccgacggcga
     4861 gttgccgcca ctgatcgtca cgagccacgg cggcccgacc gcggccgcgt tctcgggctt
     4921 cgcgaccggc ctccagctgt tcacgagccg cggctatgcc gtgctggacg tggactacgg
     4981 cggttcgacc ggctacggcc gcgactaccg gaagcggctc gagggccagt ggggcatcgt
     5041 cgacgtggac gactgcgtcg ccggcgccaa gtacctcacc gaacaggggc tggtcgacgg
     5101 cgagcgactc gcgatccgtg gcggcagcgc cagtggcttc acgaccctcg ctgccctcgc
     5161 cttcaccgac cagttcgacg ccggctgcac ctacttcggg atcggcgacc tgcgcgcgtt
     5221 cgtgaaggac acgcacaagt ttgaatcgcg ctacctggag tcgctgctcg gcccatggcc
     5281 ggaggcgaag caggtctacc tcgatcgatc acccagcctg cacgcggaga agatcacggc
     5341 accggtgctc gtccagcagg gcgccgagga caagatcgtt ccccaggccg aggccgagcg
     5401 gatcgtcgat gccctgttcg agcgacgcgt cccgcacgcg tacctgctct acccgggcga
     5461 ggaccacggc ttccgtggcc acgacgcgat cattcgctcg ttcggcgccg agctctcgtt
     5521 ctacgcccag gtcttcggat tcgaaaccgc cgacgacatc gagccgctcg agatccagtt
     5581 cctcgaggaa tcccggcgct ccacgtaggg tggcgtgcgc cgccgggcga gtgccagcgg
     5641 caagacccga tcgatgttca ccacatgaac agattccggg cttcgaaggg catttggccc
     5701 cgcagcggtc caacctgcga gccttcccga cggtcgtccg ccccgtttga gcgcggtttc
     5761 gagggaaagc ggacctttcg ggtggcgtag gcgccgatcc tggaggctcg cggaccgaaa
     5821 tgcctctcga agtgccagat ttgatgatgg ggtgaacatc gctgggcaga aggagtgcgt
     5881 cgggacggca aggtcgcccg tctgcctgca gatcggtccg ttcgccggtg tcggacgccg
     5941 ttttgccggg gacgagactc ggcgccacgc ggctcgtgtc gattagcccc gggcgatgcg
     6001 cgggagctgc gctcgatcgg tgatggccga gatcgcgcgc gagagccaac cggccttgcg
     6061 ggccaccatg gcgtccgtgc atgggtcgct cgcgaagggc gttccctcga acgcttgggc
     6121 gccaatggac gcggccgtac cgaccgagcg ggagagatcg ctgcccgcgt cgcccatcca
     6181 gatctcgaat cccttgttgg tccacatcat cgctacgacc tgcctttccc cttcccgtgt
     6241 gctgggcaca tcgtaggcgg cagatcgttt gatgtcaaga tacttgttgt gaagt
//
LOCUS       DATBSW010000219         7263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_338103, whole genome shotgun sequence.
ACCESSION   DATBSW010000219 DATBSW010000000
VERSION     DATBSW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338103"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VIF63_06135"
     CDS             complement(<1..427)
                     /locus_tag="VIF63_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY8238993.1"
                     /translation="MRLSLRARISVALFSAAILPLAFAWLIMWATGAGGSGGPGSPID
                     RVALFALVLAGFVGAGLALVVTSTLLAPLKDLTQAIEKAGTGERPARVEVAGEDELAR
                     LAGRYNQLTSELDRRSSALDAVRAEIAAIDPTADSTAMVT"
     gene            556..1725
                     /locus_tag="VIF63_06140"
     CDS             556..1725
                     /locus_tag="VIF63_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8238994.1"
                     /translation="MSLAVDTAVALPEIRLAHLTKRFGEVVAVDDISLTINTGEFFSL
                     LGPSGCGKTTTLRMIGGFDLPTGGTIELRGQDVSRQPPDKRPVNMVFQSYALFPHLDV
                     AGNIAFGLRRRGVDGTEIKRRTGAILELVHLPGYEKRRTHELSGGQQQRVALARALVN
                     EPNVLLLDEPLGALDLKLRKRLQVELKRIQMDVGITFVYVTHDQEEALTMSDRIAVMH
                     RGRIEQVGAPQDLYDRPQTSFVADFIGTTNLLTGTVEALRPDGALVRLDSGDTCRISS
                     DGRTAGQPVQVSIRPESIELRPADRAAVEASDGAGGGPEPTAASGVMTAKVEQVAYLG
                     ATVQYHIRTEKGLAMSVLAGRTGPRFESGDSVTLAWAPADALVMGEPPARLEDES"
     gene            1722..2957
                     /locus_tag="VIF63_06145"
     CDS             1722..2957
                     /locus_tag="VIF63_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8238995.1"
                     /translation="MTGQQPRAGVTFDEALTRQLRQANWSRRDFLARVAAFGAMAALT
                     QLLVACGQAGSSPSVAPATTTPTTAPSATAAAVATPTAAPTPVPEPEDELFVFNWDEY
                     IGEDTVKAFQDKYGIKVTYTPFSDEPTQIDKIQSDGKGGGYDVTDPASTWIPPFIADG
                     LIQKVDHTLIPNLANLSASWQNPGYDPGNAYSVPNYWWTTGYAWNPKKIKQDLTTWAE
                     LWDPALDQHLNMLSDMRECFAVGAFRLGLSPNTTDIGELDRILKLLQDQKPLLKDYTD
                     DDIGDITGNLVWLTHCWSGDWYQMTYDAPDTKYVIPTEGSIKGNDVMVILSGAQHPVA
                     ANLWINFNLEAEVAAANTNFTGYIGPNDAALPMIDPLISEDPRLNPPQEVLDTLVELA
                     YLQPADLAEYTDRWLTLIA"
     gene            2954..3817
                     /locus_tag="VIF63_06150"
     CDS             2954..3817
                     /locus_tag="VIF63_06150"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238996.1"
                     /translation="MIAPRPLAGRLADGLLILPGVAWLAVLFLVPVAIMFVVSLGAVD
                     PSGHVTLANPNLENYQQATRPEFLPALLRSLWYSIATTILSIGIGYPVAYWISRHGGR
                     HKILLLILVMLPFWTSYLIRTYAWMIILRENGIVNSLLLSTGLIEDPIPVLGTDASVI
                     LGMTYGFLPYAILPLFVSIDRLDENLVKAARDLYASGRRAFLHVTLPLTMPGIIAAAL
                     LTFIPALGDWVTPDLLGSAQTTTIAKLVQVIFTTGRDWPYGSALGFLLMAVTLFGTLA
                     ALRSLRREVIR"
     gene            3814..4629
                     /locus_tag="VIF63_06155"
     CDS             3814..4629
                     /locus_tag="VIF63_06155"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8238997.1"
                     /translation="MRDKSSRWLTAFTIACYAFLFAPIVVLIVFSFNQSRRNFVWQGF
                     TIDWYEKLLGNDGLIDALWVTMQVAAIAVVGSTVLGSLLGLGLARLRFRGSGAVETLL
                     LLPMVTPEIIMGISLLVFFVQLFSLNGSIGQIALAHITFCISYVAITVRARAANLNPQ
                     LEEAARDLGASALGAFRHVTLPLLAPAIAAGAMLAFALSFDDLIVTSFNAGVGSATLP
                     IAIYSSIKFGVTPQINAISTIIVAIVSLILLIAWRLGAFRNAARAVIAEPEPT"
     gene            complement(4626..5807)
                     /locus_tag="VIF63_06160"
     CDS             complement(4626..5807)
                     /locus_tag="VIF63_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8238998.1"
                     /translation="MTATATRTAKPTSSTLYFGPWYRRSPFFEKTLEAGCSAYDIYNH
                     MYLPGYYADPVEEYWALLNDVTVWDVSVERIVEITGPDSSEFINRLTCRDLTKCAVGQ
                     GKYVLITAEDGGIVNDPVLLRVEENRWWLALADSDTGLYARGVAVNSGLDVKVGEPDV
                     YPIQVQGPKSKDVLHALFGDAILDVKYYWTLTTELDGIPVVISRTGWTGEVGYEIYLR
                     DPSRGADLWDRVFEAGKPYNIRPVAPVEARRIEAGIFNYGSDMNISNNPFEVMGLERL
                     VEEQEADYIGKAALERIRREGVSKKLVGIEAPGAALPFELAEKRPALHRGESVGTVTD
                     LIWSPRLEKNIGYVWLPIELSGAGTELEIVAPDGELWPARSAAIPFLDATKKVPLGQT
                     A"
     gene            complement(5859..>7263)
                     /locus_tag="VIF63_06165"
     CDS             complement(5859..>7263)
                     /locus_tag="VIF63_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY8238999.1"
                     /translation="EGAALVSRSRRLGRRVGQGRTAVVTVSTGEGSLIADLAPRLGLS
                     LPPIPADARARIRAAMPTLSYVGNPIDPWGAGDATPTYRATFDALADSGAYDVVALVH
                     DFPFGSPRSEVDLALELAAELVASAADRAAVLPVFVSLVSGDVTPEVEAQMDAAGGVP
                     ILRGLQNGFGAIAKVAWWEGRFVERARRGPVRATWPALATDVPAYGFDRPAGHEPAGS
                     RSTPVAAAPAVPARVIPERESLELLRAEGVRVVESIAVEGRVATTMLAAATAAADRLG
                     WPVAVKLDAPGLAHKTDVGAVELAVTDTKKLAGALRRVLNAGREYEPDGVLIQPMARA
                     GVELIVGARRDPQFGPLVLVGLGGIHAEVLDDVALRLAPIDATDARAMLDELRGTRLL
                     GPIRGRRAIDRQAVVDLLVALSRAVVAHPEWREVDLNPVLAATAPHGALAVDALIVTD
                     PVDPAWDFEDPGGPAHA"
BASE COUNT         1150 a         2531 c         2450 g         1132 t
ORIGIN      
        1 ccgtgaccat tgccgtcgaa tcggcggtcg gatcgattgc ggcgatctcg gcccgtacgg
       61 cgtcgagcgc actactccgc cggtccagct ccgacgtgag ctggttgtag cggccggcga
      121 ggcgcgcaag ctcgtcctca cccgccacct cgacgcgggc cggccgctcg ccggtccccg
      181 ccttctcgat ggcctgggtg aggtccttga ggggcgccag gagcgtcgag gtcacgacga
      241 gcgccagtcc agctccgacg aagccggcga ggacgagcgc gaacaacgcg acgcgatcga
      301 tcggtgagcc ggggccaccc gatccgcccg cgcccgtcgc ccacatgatc agccaggcga
      361 acgcgagcgg caggatcgcg gcactgaaga gcgcgaccga gatccgggcg cggagggaga
      421 gcctcatgcg tggcttcctg tctgcatcat caccgcgagc atatcgggcg cctcgcgtga
      481 tcgaccgcac cgccgtcggg gacgcccggg cgacggctgt tgcgcacctg ctcaataggc
      541 taccctcggc gggtcgtgag tctggccgtc gacaccgcgg ttgccctgcc cgagattcgc
      601 ctcgcgcatc tcacgaagcg attcggtgag gtggtcgccg tcgatgacat ctcgctcacc
      661 atcaacaccg gcgagttctt ctcgctcctc ggcccgtcgg gctgtggcaa gacgacgacc
      721 ctgcggatga tcggcggctt cgacctgccg accggcggga cgatcgagct gcgcggccag
      781 gacgtcagca ggcagccacc cgacaagcgc ccagtcaaca tggtcttcca gagctacgcg
      841 ttgttccccc acctcgacgt ggcgggcaac atcgccttcg ggctgcggcg gcgaggcgtt
      901 gacggaaccg agatcaagcg tcgcactggc gccatcctcg agctggtcca cctcccgggc
      961 tacgaaaagc ggcgcaccca cgagctgtcc ggcgggcaac agcagcgagt cgcgctcgcg
     1021 cgggcgctcg tgaacgagcc caacgtcctg ctcctcgacg aaccgcttgg cgcgctggac
     1081 ctcaagctcc gcaagcgcct ccaggtcgag ctcaagcgga tccagatgga cgtcggcatc
     1141 acgttcgtgt acgtgaccca cgaccaggag gaggcgctga cgatgagcga ccggatcgcg
     1201 gtcatgcatc ggggcaggat cgagcaggtc ggcgcgcccc aggacctgta cgaccgcccc
     1261 cagacatcat tcgtcgccga cttcatcggc acgaccaacc tgctgaccgg gaccgtcgag
     1321 gcgctgcgac cggacggcgc cctcgttcgc ctcgactctg gcgacacgtg ccgaatctcg
     1381 tctgacggcc ggacggccgg ccagccggtc caggtcagca tccggccaga atccatcgag
     1441 ctccggccgg ccgatcgcgc cgcggtggag gcctcggacg gtgcgggcgg tggccccgag
     1501 cccaccgcgg ccagtggtgt gatgaccgcg aaggtcgagc aggtggcgta cctcggcgcc
     1561 accgttcaat atcacattcg caccgaaaag ggcctcgcaa tgtcggttct cgcaggcaga
     1621 acgggtccca gattcgaatc cggagattct gtgacgttgg cttgggctcc ggcggacgcc
     1681 ctcgtgatgg gcgagccgcc ggcaagactg gaggacgagt catgacggga cagcagccgc
     1741 gggcgggcgt cacgttcgac gaggcgctca cgagacagct caggcaggcg aactggtcgc
     1801 gacgcgactt cctggcgcgt gtcgccgcgt tcggcgcgat ggccgcgctg acgcagctgc
     1861 tcgtcgcgtg cggtcaggcc ggctccagcc catcggtcgc acctgcgacc accacgccga
     1921 cgaccgctcc cagcgccacg gcagccgctg tcgccacgcc gaccgccgcg ccgacgcccg
     1981 tccccgaacc agaggacgag ctcttcgtct tcaactggga cgagtacatc ggcgaagaca
     2041 cggtcaaggc cttccaggac aagtacggga tcaaggtcac atacacgccg ttctcagatg
     2101 aacccaccca gatcgacaag atccagagcg acggcaaagg gggcggctac gacgtcaccg
     2161 accccgcatc cacctggatc ccgccattca ttgccgatgg cctgatccag aaggtcgacc
     2221 acaccctgat cccgaacctc gccaacctgt cagcgtcgtg gcagaacccg ggctatgacc
     2281 ccggcaacgc gtactcggtg ccgaactact ggtggaccac cggctacgcg tggaacccaa
     2341 agaagatcaa gcaggacctc accacgtggg cagagctctg ggaccccgcc ctcgatcagc
     2401 acctcaacat gctcagtgac atgcgcgagt gcttcgcggt tggagcgttc cgactcggcc
     2461 tgtcgccgaa tacgaccgat atcggcgagc tggaccggat cctcaagctc ctccaggacc
     2521 agaagccgtt gctcaaggac tacacggacg acgacatcgg cgacatcacc ggcaacctcg
     2581 tgtggctgac ccattgctgg tcaggcgact ggtaccagat gacctatgac gcccctgaca
     2641 cgaagtacgt catcccgacg gagggctcga tcaagggcaa tgacgtgatg gtgatcctgt
     2701 ccggcgccca gcatccggtc gcggccaacc tctggatcaa cttcaacctc gaggcagagg
     2761 ttgccgccgc gaacacgaac ttcaccggct acatcggacc caacgatgcc gccctgccga
     2821 tgatcgaccc gctgatcagc gaagacccgc gactcaaccc gccgcaggag gtgctcgaca
     2881 cgctggtcga gctcgcctac ctgcagccgg ccgacctcgc ggaatacacc gaccgctggt
     2941 tgaccctcat cgcgtgatcg cgccccgccc gctcgcgggt cgcttggccg acgggctcct
     3001 gatcctgcca ggcgtcgcct ggctggccgt gctgttcctc gtcccggttg cgatcatgtt
     3061 cgtcgtcagc ctcggcgcgg tggacccgag tggccacgtc acgctcgcga acccgaacct
     3121 ggagaactac cagcaggcga cgcgacccga gttcctgccg gcgctcctcc gctcgctctg
     3181 gtactcgatc gcgacgacga tcctctcgat cggaatcggc tacccggtcg cgtactggat
     3241 ctcgcgtcat ggcgggcgtc acaagatcct gctgctgatc ctggtcatgc tcccgttctg
     3301 gaccagctac ctgatccgga cgtacgcgtg gatgatcatc ctgcgcgaga acgggatcgt
     3361 gaactcgctg ctcctgagca ccggcctgat cgaggatccc atccccgtcc tcggcacgga
     3421 tgcctcggtg atcctgggca tgacctacgg cttcctgcca tacgccatcc tcccgctgtt
     3481 cgtctcgatc gaccggctcg acgagaacct ggtcaaggcc gcccgggacc tgtatgcgtc
     3541 gggccggcgc gcgttcctcc acgtgaccct gcccctgaca atgccgggga tcatcgccgc
     3601 tgccctgctc acgttcatcc ccgccctcgg cgactgggtg acaccggacc tcctcggcag
     3661 cgcccagacc acgacgatcg ccaagctcgt ccaggtcatc ttcacgacgg gtcgggactg
     3721 gccgtacggc tcggcgctcg ggttcctgct gatggcggtg accctcttcg gcacgctcgc
     3781 cgcgttgcga agcctgcgac gcgaggtgat caggtgaggg acaagtcgag ccgctggctg
     3841 accgccttca cgatcgcctg ctacgcgttt ctcttcgcgc cgatcgtggt cctcatcgtg
     3901 ttcagcttca accagtcccg gcgcaacttc gtctggcagg gcttcacgat cgactggtac
     3961 gagaagctgc tgggcaatga cggcctcatc gacgcgctgt gggtgacgat gcaggttgcg
     4021 gcgattgcgg tcgtcggctc gactgtgctc ggctcgctcc tcgggctcgg cctggctcgg
     4081 ctgcggtttc gcggctcggg cgccgtcgag acgctgctcc tcctgccgat ggtgaccccc
     4141 gagatcatca tgggcatcag cctgctggtg ttcttcgtcc agctgttcag cctcaacggc
     4201 tcgatcggcc agatcgcgct ggcccacatc accttctgca tctcgtacgt cgcgatcacc
     4261 gtgcgggcgc gagcggcgaa cctcaacccg cagctcgagg aggccgcgcg cgacctgggc
     4321 gcgtcggcac tcggcgcgtt ccggcacgtg acgttgccgc tcctcgctcc cgcgatcgcc
     4381 gcaggcgcga tgctcgcgtt cgcgctgtcg ttcgacgacc tgatcgtgac ctcgttcaac
     4441 gccggcgttg gctcggccac gctgccgatt gcgatctaca gctcgatcaa gttcggcgtg
     4501 acgccgcaga tcaacgcgat ctccacgatc atcgtggcga tcgtttcgct gatcctgctg
     4561 atcgcgtggc gactcggcgc gtttcgcaac gcggcccggg ccgtgatcgc cgagcccgag
     4621 ccaacctagg cggtctggcc gagcggcacc ttcttggtcg cgtccaggaa cgggatcgcc
     4681 gcgcttcggg ccggccacag ctcgccgtcg ggagccacga tctccagctc cgtgccggcg
     4741 cccgagagct cgatcggcag ccagacgtag ccgatgttct tctcgagccg cggcgaccag
     4801 atgagatcgg tgacggtgcc gacggactcg ccgcggtgca gcgccgggcg cttctcggcc
     4861 agctcgaacg gcagcgcggc gcccggcgcc tcgatgccca cgagcttctt cgaaacgccc
     4921 tcgcggcgga tccgctcgag ggccgccttg ccgatgtagt cggcctcctg ctcctcgacg
     4981 agccgttcca ggcccatcac ctcgaacggg ttgttcgaga tgttcatgtc cgagccgtag
     5041 ttgaagatgc ccgcctcgat gcggcgcgcc tccaccggcg cgaccggccg gatgttgtac
     5101 ggcttgccgg cctcgaagac gcggtcccag agatcggcgc cgcgtgacgg atcgcgcagg
     5161 tagatctcgt agccgacctc gcccgtccag ccggtgcggc tgatgacgac ggggatgccg
     5221 tcgagctccg tcgtgagggt ccagtagtac ttgacgtcga ggatcgcgtc gccgaagagc
     5281 gcgtgcagga cgtccttcga cttcgggccc tgcacctgga tcgggtagac gtcgggctca
     5341 ccgaccttca cgtcgaggcc ggagttgacc gccacgccgc gggcgtacag gccggtatcc
     5401 gagtcggcga gggcgagcca ccagcggttc tcctcgacgc gcagcagcac cgggtcgttg
     5461 acgatgccgc cgtcctcggc ggtgatcagc acgtacttgc cctggccgac ggcgcacttg
     5521 gtcaggtcac ggcaggtcag gcggttgatg aactcggacg agtccgggcc ggtgatctcg
     5581 acgatccgct cgaccgagac gtcccacacg gtgacgtcgt tgagcagcgc ccagtactcc
     5641 tcgaccgggt cggcgtagta gccgggtagg tacatgtggt tgtagatgtc gtaggcggag
     5701 cagcccgcct cgagggtctt ctcgaagaac ggcgagcggc gataccaggg gccgaagtag
     5761 agggtgctgg aggtcggctt ggcggttcgg gtggcggttg cggtcatgat cggtctccgt
     5821 tgcgggtcgt cgacggggcg cggaggtgcg cggcgggttc aggcatgggc aggaccgcca
     5881 gggtcttcga agtcccaggc agggtcgacc gggtcggtga cgatcagcgc atcgacggcg
     5941 agcgcgccgt ggggcgcggt cgcggcaagc accgggttga ggtcgacctc gcgccactcg
     6001 gggtgggcca cgacggcgcg gctcagggcg accaggagat cgacgacggc ctggcgatcg
     6061 atggcccggc ggccacggat cgggccgagg aggcgcgtgc cacgcagctc gtcgagcatc
     6121 gcgcgggcgt cggtggcgtc gatcggcgcc aggcgaagcg cgacgtcgtc gaggacctcg
     6181 gcatggatcc cgccgagccc gacgagcacg agcggcccga actgcgggtc gcgccgcgcg
     6241 ccgacgatca gctcgacgcc ggcgcgggcc atcggctgga tgaggacgcc gtccggctcg
     6301 tactcgcggc cggcattgag cacgcggcga agcgcaccgg cgagcttctt cgtgtcggtg
     6361 accgcgagct cgacggcgcc gacgtcggtc ttgtgcgcga gccccggcgc gtcgagcttc
     6421 acggcgaccg gccagccgag ccgatcggcg gcagcagtgg cggcggcgag catggtggtc
     6481 gcgacacggc cctcgacggc gatcgactcg accacgcgaa cgccttcggc gcgcaggagc
     6541 tcgagcgatt cgcgctccgg gatgacgcgt gcgggcacgg cgggggcggc ggcaactggc
     6601 gttgagcggc tcccggcagg ttcatgtccc gcggggcggt cgaacccgta ggcgggcacg
     6661 tccgtggcga gcgccggcca ggtggcacgc actgggccgc ggcgcgcgcg ctcgacgaag
     6721 cgcccttccc accacgccac tttggcgatg gcgccgaagc cgttctggag gccgcgcagg
     6781 atcggcacgc cgccggcggc gtccatctgt gcttcgactt cgggcgtgac gtcgcccgag
     6841 accagcgaca cgaacaccgg caggaccgcc gctcgatcgg cagccgatgc gacgagctcc
     6901 gcggcaagct cgagcgcgag atcgacctcg ctgcgcggcg agccgaacgg gaagtcgtgc
     6961 acgagcgcga ccacgtcata ggcacccgag tcggcgaggg cgtcgaacgt cgcgcgatac
     7021 gtgggtgtcg cgtcgccggc gccccaggga tcgatcgggt tcccgacata gctgagcgtc
     7081 ggcatggcgg cgcgaatccg ggcgcgagcg tcggccggaa tcggtggcaa cgacaagccg
     7141 agtcggggcg cgaggtcggc gatgagcgag ccctcgccgg tcgagaccgt gaccacggcg
     7201 gtcctcccct gccccacacg gcgcccgagc cggcgcgagc ggctgaccag cgccgcgccc
     7261 tcg
//
LOCUS       DATBSW010000220         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_267859, whole genome shotgun sequence.
ACCESSION   DATBSW010000220 DATBSW010000000
VERSION     DATBSW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267859"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..820
                     /locus_tag="VIF63_06170"
     CDS             119..820
                     /locus_tag="VIF63_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY8239000.1"
                     /translation="MLVIPAIDVEKGRSRVVFWPGVSTGIGAPTDRPERIAEHFVAQG
                     APLIHVIDFDGARRGAPVNTDVIGAIAARVATPLQLAGGLDSPEAIGVAFASGATRVV
                     TTLAVADDAPLLRACLEIAGDWLAVGLDARPDRLAAFRWRRAAPPTLAALISELCGRG
                     VRRFVLGHATGDAEVAEIASLVRTVDAELVVAGGVSDLDGVRRVRDMGAAGIILGEAL
                     LSGAIDYQTAREAAA"
     gene            907..1812
                     /locus_tag="VIF63_06175"
     CDS             907..1812
                     /locus_tag="VIF63_06175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8239001.1"
                     /translation="MALTGGEPTAPGAVLAGAAVRPGSRSANRAVKRATSPRPKARPG
                     NRNRNSNTGLIVLAVAAVAIGAAVLLIGNPFGKPTPSASPSAAPVLGDGTCPTSQPAA
                     LAATDKRTVTIETAKGTIVMSIDGARAPIATGNFVALATCHFYDGITFHRLVPGFVIQ
                     GGDPDGDGGGGPGYTIKDDPLIGTYHRGTVAMARTGDPDSQGSQFFIVLSDDANAPLT
                     DPQNAPYPYAIMGEVISGMEVVDAIAAMPNEGSPGNAAVDPVVMTKVTVSAGTSPTTA
                     PTTAPATTAPATPGATPAPSVTTAP"
     gene            1829..2320
                     /locus_tag="VIF63_06180"
     CDS             1829..2320
                     /locus_tag="VIF63_06180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8239002.1"
                     /translation="MTKATIATEHGDIEVDLFTKAAPKATQNFIDLAKKGFYDDVIFH
                     RVIPGFVIQAGDGQHGKKASLNAARVGTGGPGYTFEDEPVLGDYGRGSLAMANAGPNT
                     NGSQFFICVADLSGRLPKNYTLFGQVTKGLDVVDKIVGAPRNSRDLPDAPVAMTSVTI
                     EED"
     gene            complement(2657..>2933)
                     /locus_tag="VIF63_06185"
     CDS             complement(2657..>2933)
                     /locus_tag="VIF63_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY8239003.1"
                     /translation="PLAFDAGRADFTGITPVDDLCISAVIHQANIDLDEEGTEAAAAT
                     GVIAGVTSMPPEPVPMTVDRPFLFALRDVETGAILFLGRVVEPAERS"
BASE COUNT          455 a         1071 c          986 g          421 t
ORIGIN      
        1 ttctcgctcc gggccttcca cgacacgctg ctccagggcg ggtccctgcc gatcagcttc
       61 catcggcggg cgctgcacgg cgaagcctcg tcgcccccgg ccccggcctc ctagccgcgt
      121 gctggtcatc cccgcgatcg atgtcgagaa gggtcgttcc cgcgtcgtct tctggcccgg
      181 cgtatcgacg ggcatcggcg caccgaccga ccggccggag cggatcgcgg agcacttcgt
      241 cgcccagggc gccccgctga tccacgtcat cgacttcgac ggcgcccggc gcggggcgcc
      301 ggtcaacacg gacgtcatcg gcgcgatcgc cgcgcgcgtc gccacgccgc tccagctggc
      361 cggcggcctc gattcgcccg aagcgatcgg cgtcgccttc gcgtccggtg cgaccagggt
      421 cgtaaccacg cttgcggtcg ccgacgacgc cccgctcctg cgggcgtgcc tcgagatcgc
      481 gggcgactgg ctcgccgtcg ggctggatgc gcgacccgat cgactggccg ccttccgctg
      541 gcgccgcgct gccccgccga ccctcgccgc cctcatctcg gagctctgtg ggcgcggcgt
      601 tcgccggttc gtgctgggac acgcgacggg cgacgcggag gtcgcggaga tcgcctcgct
      661 cgtgcggacc gtcgatgccg agctcgtcgt cgccggtggc gtctcggacc tcgacggcgt
      721 gcgtcgcgtg cgcgacatgg gcgcagcggg catcatcctc ggcgaggcgc tcctgtccgg
      781 ggcgatcgac taccagaccg cccgtgaggc tgccgcatga cgtccccgcg ccgaccaagc
      841 cgtcgaatcc cgtcgaagtc cgggcgcgga ccgcgtcgac ccgatccgat gtcggcggat
      901 cctggcgtgg cgctgaccgg tggtgagccc acggcgccgg gcgcggtgct ggccggtgcc
      961 gcggtgcgac cgggctcgag gtccgcgaac cgcgcggtga agcgtgcgac gagcccgcgg
     1021 ccgaaggccc gcccgggcaa ccggaaccgc aactcgaaca ccggcctgat cgtgctcgcc
     1081 gtcgccgcag tggcgatcgg cgcggccgtc ctgctcatcg gcaacccgtt cggcaagccg
     1141 accccgagcg cgagcccgag cgcggcgccg gtcctcggcg acggcacatg ccccaccagc
     1201 cagccagcag ccctcgcggc aaccgacaag cggaccgtca cgatcgagac cgcgaagggc
     1261 acgatcgtca tgagcatcga cggggcgcgc gcgccgatcg caaccggcaa cttcgtggcc
     1321 ctggccacgt gccacttcta cgacgggatc accttccacc ggctcgtgcc cgggttcgtc
     1381 atccagggcg gcgacccgga cggcgatggc ggcggcggac ccggctacac gatcaaggac
     1441 gacccgctca tcgggacgta ccaccgcggc acggtcgcga tggcccggac cggggacccg
     1501 gacagccagg ggtcgcagtt cttcatcgtc ctgtcggacg atgcgaacgc tccgctgacc
     1561 gacccacaga acgccccgta cccgtacgcg atcatgggcg aggtgatctc cggcatggag
     1621 gtcgtcgatg cgatcgccgc catgccgaac gaagggtcgc ccggcaacgc cgcggtcgat
     1681 cccgtggtga tgaccaaggt caccgtctcc gcggggacat cgccgacgac cgctcccacc
     1741 acagcccctg cgaccacagc ccctgcgacc ccgggcgcga cgcccgcgcc atccgtcacc
     1801 accgccccct gagcacgagg agccacccat gaccaaggcc accatcgcga ccgagcacgg
     1861 cgacatcgag gtcgacctgt tcacgaaggc cgccccgaag gcgacccaga acttcatcga
     1921 cctcgcgaag aagggcttct acgacgacgt gatcttccat cgcgtcatcc cgggcttcgt
     1981 catccaggcc ggagacgggc agcacggcaa gaaggccagc ctgaatgccg cgcgcgtcgg
     2041 gaccggcggc ccgggctaca cgttcgagga cgagcccgtg ctcggcgact acggccgcgg
     2101 atcgctggcg atggccaacg ccggcccgaa caccaacggc agccagttct tcatctgcgt
     2161 cgccgacctg tccggccggc tgcccaagaa ctacacgctg ttcggtcagg tgaccaaggg
     2221 cctggacgtc gtcgacaaga tcgtcggtgc tccgcggaac agccgcgacc tcccggatgc
     2281 gccggtcgcg atgacgtccg tgacgatcga ggaggactga caggacattg caaccgccca
     2341 cccacgtttg ggtggctgca atgcagctgt aacgccgcgg caacgcggcg agcaccgagc
     2401 gttaagggat tttcaccaac ttcgcgacga aaacccttgg attagtcgac tggttcgact
     2461 agacttcggg agcactgcgt tcgcgcagcg tccgctgccc cagcggcaag gtcatgggta
     2521 ggttgggtca gggtagtcgg tgcagccgac gtgatagatg cccttggcgc gtggcagaag
     2581 cgtccgtttc ggcggatacc ttcaccggga cagcatcgga gcggctcctt cgggagccgc
     2641 tccgaacttt ttccggctag ctccgctcgg caggctcgac gacacgtccc aggaatagga
     2701 tcgcgcctgt ctccacgtca cggagcgcga acaggaacgg ccgatcgacg gtcatcggca
     2761 cgggctccgg gggcatcgag gtcacaccgg cgatgactcc tgtcgccgcg gctgcttcgg
     2821 tgccctcctc atcgagatcg atgttggcct ggtggatgac ggcgctgatg cacaggtcat
     2881 ccaccggtgt gatgccagtg aagtcggcgc gccctgcgtc gaatgccagg ggc
//
LOCUS       DATBSW010000221         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_514634, whole genome shotgun sequence.
ACCESSION   DATBSW010000221 DATBSW010000000
VERSION     DATBSW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514634"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..781
                     /locus_tag="VIF63_06190"
     CDS             167..781
                     /locus_tag="VIF63_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239004.1"
                     /translation="MRRSLVLLGSAFFLAACTGTPPPVPTATPIAAQPTPTTAPPLAS
                     PRPTPTPVSGQGICPGGSPLTVVEFSNSDPACFGSADVEIRGWLDFIPDMGFEAPLVE
                     PTWIYYPTANRAIWSEERGPDQTCLGAAAACTWFFAHADPAAGLDLDRKPGWVLLTGH
                     LDDPAARTCHFVYTEDWTEPPLDNADAVATCRSRFVIVSFSDAP"
     gene            837..1487
                     /gene="lexA"
                     /locus_tag="VIF63_06195"
     CDS             837..1487
                     /gene="lexA"
                     /locus_tag="VIF63_06195"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8239005.1"
                     /translation="MTRHDADRKLKILHYIAGTLRSRGYPPSVREIAVAVGLSSTSAV
                     HHHLQMLERDGFLERRESMSRAIRMTPTAAIELGLTHELVPQSVTAEAHVLPVVGEIA
                     AGGPIEAYQDASETMAVPDLLAPSGDAYVLRVRGDSMIEEHIADGDFVLIRPQTTARN
                     GDIVVAQVEENAVTLKRFFREKDKIRLQPANPNYAPQFYDDVRIQGKLIGVIRRLD"
     gene            1541..>1764
                     /locus_tag="VIF63_06200"
     CDS             1541..>1764
                     /locus_tag="VIF63_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239006.1"
                     /translation="MEPKIEITKSGRRLTEADIERMADEAERGFDLSTFRHSRGRPRL
                     EVGQAGSSRIAVRVPRSLHRRVTSRAAAEGR"
BASE COUNT          288 a          644 c          542 g          290 t
ORIGIN      
        1 gatcacgtcg aacgcgtcgg ccgacctgcg cttctcgcga gccacggctc gctcacctcc
       61 tgggccatcc cgtaccgccc gcggcggctc ctccgggatc gggaagtcta tcagcgcgtc
      121 ccgtcgcatc cgacaatgtg attcgtccta tgcccgggag ggtttgatgc gtcgatcgct
      181 ggtcctgctc ggttccgcat tctttcttgc agcgtgcacg ggcacgcccc cgcccgtgcc
      241 gacggcgacg cccatcgccg cccaaccgac cccaaccact gctccgccgc ttgcgtcgcc
      301 tcggccgacg ccgacaccgg tctcgggcca gggcatctgc ccaggcgggt cgccgcttac
      361 ggttgtcgag ttctcgaatt cggatcccgc gtgcttcggc agcgccgatg tggagatccg
      421 gggctggctc gactttatcc ccgacatggg gttcgaggcg ccgctcgtcg aaccgacctg
      481 gatctactac ccgaccgcga atcgcgccat ctggtcggag gagcgcggcc ccgaccagac
      541 atgcctgggc gccgctgcgg cgtgtacgtg gttcttcgcc cacgccgacc ctgctgccgg
      601 tctcgacctc gacaggaagc ctgggtgggt cctcctcaca ggccatctcg acgaccccgc
      661 tgccaggacg tgccacttcg tctacacgga ggattggacc gagccgcccc tcgacaacgc
      721 cgatgcggtt gcgacatgtc ggagccggtt cgtgatcgtc tcgttcagcg acgcgccttg
      781 acatcgaaca cccgttcggt atgatctcac tggccactgc cctacgggag tcccgagtga
      841 cgcgacacga cgctgaccgg aagctgaaga tcctccacta catcgccggc acgctgcgct
      901 cccggggcta tccgccctcg gtccgcgaga tcgccgtcgc ggtcgggctc agctccacgt
      961 ccgccgttca tcaccacctg cagatgctcg agcgagatgg attcctcgag cggcgcgagt
     1021 cgatgtcgcg cgcgatccgc atgacgccga ccgccgccat cgagctgggt ctcacccacg
     1081 aactggtgcc ccaatctgtg accgccgaag cgcacgtcct gccggtcgtc ggcgagatcg
     1141 ccgccggcgg cccgatcgag gcctaccagg acgcctcgga gacgatggcc gtcccggacc
     1201 tgctcgcacc gagcggggac gcgtacgtcc tgcgcgtccg cggcgactcg atgatcgaag
     1261 agcacatcgc cgacggcgac ttcgtcctga tccgcccgca gaccacggcc cgcaacggcg
     1321 acatcgtcgt cgcccaggtc gaggagaacg cagtcaccct gaagcggttc ttccgcgaga
     1381 aggacaagat ccgcctccag cccgcgaacc ccaactacgc accgcagttc tacgacgacg
     1441 tgcgcatcca gggaaagctc atcggggtca ttcgcagact cgattaggct cgctaacccg
     1501 cggcaatcgt tagcgtcggc atttgtccga cgaaacctcg atggaaccaa agatcgagat
     1561 caccaagagc ggtcgtcgcc tgaccgaggc cgacatcgag cggatggcag acgaggccga
     1621 acgaggattc gacttgtcga cgttccgaca cagccgcgga cggccgcggc tggaagtcgg
     1681 gcaggcgggc tcatcgcgga tcgccgtccg ggtcccacgt tcgcttcacc gacgcgtcac
     1741 ctcgcgtgcc gccgccgaag gtcg
//
LOCUS       DATBSW010000222         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_901961, whole genome shotgun sequence.
ACCESSION   DATBSW010000222 DATBSW010000000
VERSION     DATBSW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901961"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..1198)
                     /gene="rpoD"
                     /locus_tag="VIF63_06205"
     CDS             complement(353..1198)
                     /gene="rpoD"
                     /locus_tag="VIF63_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY8239007.1"
                     /translation="MAGKEAELLKLMGHDPEVPKNTKLAHRKGELVRIGRDAREQLTS
                     ANLRLVVSIAKKYIGRGMSFLDLIQEGNIGLIRAVEKFDFEKGFKFSTYATWWIRQAI
                     TRAIADQARTIRIPVHMVETINRLIRVSRQLLQELGREPTVEEIAEAMSKGQEVQVTP
                     EKVREIIKVSQEPVSLETPIGEEEDSHLGDFIEDRGALAPAEAASHQLLKEQVESVLD
                     SLTGRERRVLQLRFGLEDGRARPLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSRKL
                     KDYLE"
     gene            complement(1655..2110)
                     /locus_tag="VIF63_06210"
     CDS             complement(1655..2110)
                     /locus_tag="VIF63_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEY8239008.1"
                     /translation="MPSDPLDQQLVEAVLTRPRRGKADLEEAKLAGLRPSRAALDDVD
                     DDEDEDEDEPDVALAGDDGDDDLTVEVVGEDADARTSKLPADAPAKLDPEELEEPTAA
                     ELEALSADMIGIDDPVRMYLKEIGKVALLTAEEEVVLAKAIELGEQLVD"
     gene            complement(2117..4153)
                     /gene="dnaG"
                     /locus_tag="VIF63_06215"
     CDS             complement(2117..4153)
                     /gene="dnaG"
                     /locus_tag="VIF63_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8239009.1"
                     /translation="MFRNVGIDDEPGGYFELAGGIAAEVKSRLNIVDVIGETVQLKKA
                     GTTFKGLCPFHGEKTPSFTVTPSRDSWKCFGCGLGGDVFSFVMQRDGSTFPDALKSLA
                     AKAGVEIDERTKREDMHRARLRDVMETAIAFYHQVLTTTNMGKPALDYLKGRGFTDET
                     ISTFQLGWAPEGWDSMSRKLVEKKGIKPEELVEAGLASVARSPQRGVGVIDKFRGRVL
                     WPIRDQNGAATGMGGRILGSEGEGGRDTGPKYLNTPGTPLFDKSRSLYLIDKAKSAMR
                     KSGQAVIVEGYTDALMAHQAGFDNVVASLGTALTPGQVALLTRYAKRIALAYDVDAAG
                     QKAGTFGVQALQGLIGQLAATESGIELDEVRVVRMPAGKDPDEVLRDTPDRWREEVRT
                     AQPIVEYLVDTYAKAYDLKTPGGKARFVDALMPTIRAVPNPVMRDAYLQLVHRVSGVE
                     ERVLLEVLRGTRLGGASGLASAQHGQGANGMGGGSDAGREGRFTADAVITSADSLPVS
                     EILRGVLPQEAEFLRLLLLVPELQLKIVDAIGPDQLPSTVARELFRAVVLAREPNDEG
                     VHPPFSLAALIQALDPETAALAQAVVARRDPNPRSLSEADLVYEVERLTIDLEERSLD
                     ERADYIASALAEAEQSADRPSIDQLLRESQAMNEQRKSLHLRRNQTHLLTATRR"
BASE COUNT          565 a         1472 c         1476 g          756 t
ORIGIN      
        1 cgcgggcatc gccaggtggc ccgccgacgg cgacgaacag cgccgcggtg accgcgattg
       61 ccatcgcggc gcgcgctatg cgttgcccat cgatcctcac ccgaatccct cttcccctcg
      121 ccacccgcca gtcggtcgac cgccctccgc tcggtcacga ggttggcggc acgatagtcc
      181 cgtcacgtct cgctgacaat cgcgaatttc gggctcccga ccgcggttct gacgagttgg
      241 cttggtcgtg tcttgatcgt gtcccggcat gcgtcggtcg catgcgccgt ggtagcgccg
      301 ccgcagctcg cgatgcgtgg cgagcggcgg ggtgacccgc cgctcccctg ctctactcca
      361 ggtagtcctt gagcttgcgg ctgcgggatg ggtggcggag cttgcggagg gccttcgcct
      421 cgatctggcg gatgcgctcg cgggtcacgt tgaactcctt gcccacttcc tcgaggggcc
      481 gggcacggcc gtcctcgaga ccgaagcgga gctggagcac gcggcgctcg cggcccgtca
      541 gcgagtcgag caccgactcg acctgctcct tgaggagctg gtgcgaggcg gcttcggcgg
      601 gcgcgagcgc cccccggtcc tcgatgaagt cgccgaggtg ggagtcctcc tcctcgccga
      661 tcggggtctc gagagacact ggctcctgcg agaccttgat gatctcgcgg accttctccg
      721 gcgtgacctg gacctcctgg cccttcgaca tcgcctcggc gatctcctcg accgtcggct
      781 cacggccgag ctcctggagg agctggcgcg agacgcggat caggcggttg atcgtctcga
      841 ccatgtggac cgggatgcgg atcgtccgcg cctggtccgc gatggcgcgg gtgatcgcct
      901 gccggatcca ccacgtggcg tacgtggaga acttgaagcc cttctcgaag tcgaacttct
      961 cgactgcacg gatgaggccg atgttgccct cctggatcag gtccaggaac gacatgccgc
     1021 ggccgatgta cttcttcgcg atcgacacga ccaaccgcag gttggccgag gtcagctgct
     1081 cgcgtgcgtc ccgcccgatg cggaccagct cgcccttgcg gtgcgcgagc ttggtgttct
     1141 tcgggacctc gggatcgtgg cccatcagct tgaggagctc ggcttccttg ccggccatca
     1201 cgaaccgctc cagcgccggg aacgcgacgc cgtcgcgggt ccagtcgtag atcgctcgca
     1261 ggccgacgtt gtcgcgcgag tcgagatcgc cgttgtggac cgcgaggtac gcccagtcga
     1321 gcagcgtcag gaacgcttcc ggtgcggcgc ggtgcgcgga cttgccgttc gcgccgtccg
     1381 cctcgaccgc ctcggcgtag aggtggacga gcttgcgccc ctccttcagg agctccttcg
     1441 tgccatcgga ctgcgcgtcc ttgccggccc ggttcaggtg gaagtcgggc ggcggggcga
     1501 ggaggtcggc cgcctccgtg gcggtgacgc cctcgcggac gaggcggtgc gcctcgtcgc
     1561 cgagcggcag gcggtgctgc tgcttgagcg ttcgggtctt cgtctcggtg ttgtgggtcg
     1621 tccactcgtg gagcgagacg aggcccttcc acggctagtc gacgagctgc tcgccgagct
     1681 cgatcgcctt ggccaggacg acctcttcct cggcggtcag cagggcgacc ttgccgatct
     1741 ccttcaggta catccggacc gggtcgtcga tgccgatcat gtcggccgag agggcctcga
     1801 gctccgccgc ggtgggctcc tcgagctcct ccgggtcgag cttcgcgggc gcgtcggccg
     1861 gcagcttcga tgtgcgcgcg tcggcgtcct cgccgacgac ctcgacggtc aggtcgtcgt
     1921 cgccgtcatc gccggcaagc gcgacatcgg gctcgtcctc gtcctcgtcc tcgtcgtcat
     1981 cgacgtcatc gagggcggcg cgggacggcc gaaggccggc gagctttgcc tcctcgaggt
     2041 cggccttgcc ccgccggggg cgggtcagga cggcttcgac gagctgctgg tcaaggggat
     2101 cgctgggcat ggcggcttac ctccgggtcg cggtgaggag atgggtttga ttgcggcgta
     2161 ggtgcagcga cttgcgctgc tcgttcatgg cctggctttc gcgcaggagc tggtcgatcg
     2221 atggtcggtc ggccgattgc tcggcctcgg cgagcgccga tgcgatgtag tcggcgcgct
     2281 cgtcgagcga gcgctcctcg agatcgatgg tcaggcgctc gacctcgtag acaagatctg
     2341 cctcggacag cgagcgcggg ttcggatcgc gccgggccac gaccgcctgg gcgagcgccg
     2401 cggtctcggg atcgagggcc tggatgagcg cggccaggct gaacggcggg tgcacgccct
     2461 cgtcgttcgg ctcgcgggcg aggacgacgg cgcggaacag ctcgcgcgcc acggtgctcg
     2521 gcagctggtc cggcccgatc gcatccacga tcttgagctg gagctccggc acgagcagca
     2581 gcagccgcag gaactccgcc tcctggggca gcacgccgcg gaggatctcg ctcacgggca
     2641 gcgaatccgc cgaggtgatc accgcgtcgg ccgtgaatcg accctcgcgc cctgcgtccg
     2701 agccgccacc catcccgttg gcgccctggc cgtgttgtgc ggaggcgaga cccgacgctc
     2761 ccccgaggcg cgtcccgcgc aggacctcga gcagcacccg ctcctcgacg cccgatacgc
     2821 ggtggacgag ctgcaggtag gcgtcgcgca tcaccgggtt gggcaccgcg cggatggtcg
     2881 gcatcagcgc gtcgacgaag cgggccttgc cgcccggcgt cttgaggtcg taggccttgg
     2941 cgtaggtgtc cacgaggtac tcgacgatcg gctgcgcggt gcggacctcc tcgcgccagc
     3001 gatccggcgt gtcccggagg acctcgtcgg gatccttgcc ggccggcatg cgcaccacgc
     3061 gaacctcgtc gagctcgatg ccgctctccg tggcggcgag ctggccgatg aggccctgga
     3121 gcgcctggac gccgaacgtg ccggccttct ggccggccgc atcgacgtcg tacgcaagcg
     3181 cgattcgctt cgcatagcgg gtgaggagcg cgacctggcc cggcgtgagc gccgtgccga
     3241 gcgaggcgac gacgttgtcg aatccggcct ggtgggccat cagcgcgtcc gtgtagccct
     3301 cgacgatcac ggcctggccg gacttgcgca tcgccgattt cgccttgtcg atgaggtaca
     3361 gcgaccggct cttgtcgaac agcggcgtgc ccggcgtgtt gaggtacttc gggccggtgt
     3421 cacgaccgcc ctccccttcc gagcccagga tccggccgcc catgcccgtc gcggcgccgt
     3481 tctggtcgcg aatcggccag agcacgcggc cgcggaactt gtcgatgacg ccgacgccgc
     3541 gctgcggcga gcgagccacg gaagcgaggc cggcctcgac gagctcctcg ggcttgatgc
     3601 ccttcttctc gacgagcttg cggctcatcg agtcccagcc ctcgggcgcc cagccgagct
     3661 ggaacgtcga gatcgtctcg tcggtgaaac cgcggccctt gaggtagtcg agggcaggct
     3721 tgcccatgtt cgtcgtggtc aggacctggt ggtagaacgc gatcgccgtc tccatcacgt
     3781 cgcgcaggcg cgcgcgatgc atgtcctcgc gcttcgtccg ctcgtcgatc tcgacgcccg
     3841 ccttcgcggc gaggctcttc agcgcgtccg ggaacgtcga gccgtcgcgc tgcatcacga
     3901 agctgaagac atcgccgccg aggccgcagc cgaagcactt ccatgaatcg cggctcgggg
     3961 taacggtgaa cgacggtgtc ttctcaccgt ggaaggggca caggcccttg aaggtcgtgc
     4021 ccgccttctt cagctgcacc gtctcgccga tcacgtcgac gatgttcagc cgactcttga
     4081 cttccgccgc aatgccgcct gccagctcga aatatcctcc aggttcgtcg tcgattccta
     4141 cgttccggaa cacgacgcgg ttcccgcccg ttcgtgtcgc tcgacccgcg acctcccgga
     4201 catgactgtc ggcctgtcaa gtcttgacaa gccgtggaac tgaattctac cccttgtcaa
     4261 gccctcttg
//
LOCUS       DATBSW010000223        17635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_303029, whole genome shotgun sequence.
ACCESSION   DATBSW010000223 DATBSW010000000
VERSION     DATBSW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17635
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303029"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VIF63_06220"
     CDS             <1..1234
                     /locus_tag="VIF63_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY8239010.1"
                     /translation="DSILRGDVQSPELRWRDAEGVHRSQSRPRPSPREQLAPDRFSGL
                     IGQGSSWRTEPSWRGDGGFRGDFRGDYRADPGGRGGSGSGFFPGERGSRPGFRPGASG
                     SWRPGRSPSGSLTAAAGAGAGAPRVEAPFVAGEIADRGPVERGTTPAPEPRAREAREL
                     SRQREWRESATKAIDTLKAEEGTGPVNAAELVASELAALEAAARTAELGLRAAPTPGI
                     DDLAADDGALLDGALDDDDDDDDRASGILESEPVGLPTLRLLPQGISRKRLEQAIREL
                     QLPVIIARDVDEADVVMTIRSEYKQKTPMVREAEERAMPIYVLKANTIPQMQASLTSI
                     FALEIDPRDAALRETEEAIGLVINRAEAIELSPQNAYIRRLQHQMAERANLVSRSRGR
                     EPYRRVRLYPDAAKSPWR"
     gene            1374..2633
                     /locus_tag="VIF63_06225"
     CDS             1374..2633
                     /locus_tag="VIF63_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8239011.1"
                     /translation="MHKFVANREAIVQEINGGDTAWMLIATALVMIMLPGLALFYGGL
                     VRRKNVLSTIMHSFFGLALVSVVWVLVGFSLAFGKDVTGLGLLGGSEYLGFSNVGLEP
                     NPTYGPTIPFVLFAAFQLMFAAITPALISGAFAERKRFLSFVMFTGLWSVLVYSPIAH
                     WVWATDGWLFKLGALDFAGGTVVHAASGVAALVAALAIGKRASSGDKMEPHDIPMTVL
                     GAGLLWFGWFGFNAGSALTAGGLAANALLVTNTAAAAATLTWAFASYLHRGKVSVVGA
                     ACGAVAGLVAITPASGFVTAGGAVVIGLVAGGLCYSATLLRERLRIDDALDVFAVHGV
                     GGVFGAIATGVFATTAINAFPGLVDGNPGQLVTQAIAVGATVAYSAGATFVIIKLVNF
                     VLGLRVPADEEAVGLDLAVHGEAAYQT"
     gene            complement(2648..3649)
                     /locus_tag="VIF63_06230"
     CDS             complement(2648..3649)
                     /locus_tag="VIF63_06230"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8239012.1"
                     /translation="MTRKLGIEQPIVQAPIGGLSNPRLAAAVSNAGGLGLIAMTWTEL
                     DEMRQAIRSTQALTGQPFGINLIIDEPQEERLAVALENGVKIISFFWGDPAPYIAAVH
                     DAGAIAMLTVGSAAQARAAVDAGVDVIVAQGWEAGGHVLGEVSTLALIPAVIDEVGAT
                     PVIAAGGITDGRGLAAVLALGAGAAWMGTRFVASEEAPAHPDYVARLLDASETSTYHS
                     MLFDGGWPNAAHRTLRNSTIDAWEAAGRPPSGQRPGEGEVIAVRADGSEIPRYWSVAV
                     RMGTTGDVEGLSLWAGQGVSLVHEVLPAAQIVHRTVSDAERILARLATSAEPVAERQ"
     gene            complement(3692..4156)
                     /locus_tag="VIF63_06235"
     CDS             complement(3692..4156)
                     /locus_tag="VIF63_06235"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY8239013.1"
                     /translation="MGSGEIRESVRARLGWPADAVTHRRIRRLWQRHSIAEDQRDIDG
                     LIATLAPECVYEIVGTGLRWAGHAGARAFYTELFAAFPDNQFSLTDIVIGPQGVFEAV
                     VLEATHVEAFAGLAPTGRRVSLELAILFPWDPATQRFMGEQIFIDRAALAAG"
     gene            complement(4173..4577)
                     /locus_tag="VIF63_06240"
     CDS             complement(4173..4577)
                     /locus_tag="VIF63_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239014.1"
                     /translation="MNRESEYALRSKQRMSEAQEPSRRPWTDTGQRKPPGAGDLRAWD
                     GLLVFDRIRIAIEAETRLRDAQSLERRYALKMRDGHVELMILLVSDTAHNRTFLDLHR
                     EALRTRFPLDGRQLLPELRAGRAPRDSGILVL"
     gene            complement(4696..6090)
                     /locus_tag="VIF63_06245"
     CDS             complement(4696..6090)
                     /locus_tag="VIF63_06245"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8239015.1"
                     /translation="MSDASLAGRVDSRDPATALALAEDVLERALRVGATEAEVLVLAG
                     DSALTRFANSEIHQNVAERSLTVSLRHVVGRRIAVVSTGKVDADGLRSLVHRAAAIAR
                     SCEELEDWAGLPAPLTPGGAVVRGTGGGWSSGTADATPEFRAEGVRAVIAAADAAGVM
                     AYGSFATDSEGVAVANSAGIRAAESRTSSQLLTVHMSPNGGTGYAEAVSMDATTIDAA
                     AIGREAAAKARASDNPVSVPAGDYPVVLEEYAVVDITDQLGYVGFSALAVQEGRSFAE
                     PGRRIGSDLVTIVDDGSDQNGLPMAFDYEGVPKQRVPLVEGGVCREVVFDSQTAARAG
                     RTSTGHGLPAPNPYGPFPLNAVMAAGSTPRDELIGGLDRGLLVTRFHYTNVVHPKLAI
                     VTGMTRDGTFLVEGGKLVGPVRNLRYTQSYLDALAGVTAVGDSRKTIRGFLGAAVVPA
                     LRIDGWTFTGVTEF"
     gene            complement(6087..7535)
                     /locus_tag="VIF63_06250"
     CDS             complement(6087..7535)
                     /locus_tag="VIF63_06250"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8239016.1"
                     /translation="MKDLTDRALDTAAQLGATYADVRVVRRRDESISVKSGRVEGVAL
                     GETDGFGVRVVVDGAWGFAASDTLDLAEADRVGALAVRIAKASSTALRRPVELDGRPP
                     ATGTFETPVGEDPFKIPLERKISDLVEADLAAGKVKGVTFTESMYSGHREWKTFAATD
                     GSFTEQVITQVGAAVEANAIEGDEHQRRSYPDSGGGFQAGGYDYIRSLDLQGNATRVG
                     EEAVALLSAPQCPPGMRTLVLDPSMLYLQIHESCGHPTELDRVFGTEASYAGTSFLTP
                     DKLEEGFQYGSELVTIVADATAEGGLGTFGWDDEGVAAQSTPLIQNGRLVGYQSSRET
                     APRVGRMSGGQMRADGWNRIPLIRMTNVNLLPVPGMSLDEIVADTDDGLYLANNRSWS
                     IDDRRLNFQFATEVAYEIKGGKKGRLFKNCTYTGITYEFWRSCDAVGDQSSFVMLGTP
                     NCGKGEPPQSARVGHAVSGARFRNVQVGVGKW"
     gene            7599..8228
                     /gene="tmk"
                     /locus_tag="VIF63_06255"
     CDS             7599..8228
                     /gene="tmk"
                     /locus_tag="VIF63_06255"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8239017.1"
                     /translation="MSGLFITLEGPEGGGKTVIAKRLVEELTRRGHVVRSTREPGGTR
                     LGERVRGIVLEQSADDRAAAIDRRADALLFNAARAQLVAEVIRPALDRGEVVLCARYA
                     DSTLAYQGYGAGLPLDELRALAAVATGDLKPDLTILLDVPPEIGIGRKTGASRNRFES
                     SFDLAFHERVRAGFLALAGQEPDRFRVIDSSRRIGAVIPEVVAAVLAAL"
     gene            complement(8232..9833)
                     /locus_tag="VIF63_06260"
     CDS             complement(8232..9833)
                     /locus_tag="VIF63_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239018.1"
                     /translation="MSRRRVPRPGRIAGAMAAVVVALLVPAIALAHPLGNFTINHYAA
                     LRIAPDRISLDVVIDHAEIPALQEQQRLDIDGDGVLAAAELEAARQVACGTLASDLRL
                     AVDGRALAPTLVAAGLALPPGAGGLPTMRLVCEYEAALASPIGASSTVIFEDRSFAER
                     IGWREIVVLGDGATVTIGATGAETSPAGAEGVSNRLTTYPKDLLTQPLDMRSIAVDVA
                     PGGAALGAFLAPDAQPLAAPPAPSGPGPIGAVPGGIAEDLAALVDVSDLTPLAILGSL
                     AIAFSLGIVHALSPGHGKTIMAAYLVGAKGSSRQAFGLGLAVAVSHTLGVLALAAITL
                     AASNVLPPERLYPILGVVSGGLVIAIGGSLLWSRLRAMGLGRPHQTDHGHEHGHDARG
                     HDHAHTADHAHDPDPEHGHPHPHPHPHSHVATDTTISWRGLIALGLSGGLVPSASALI
                     LLLGSIAAGRVAYGLVLVIGFGLGMAVVLAGTGLLIVNARRLIERRPSMGGLRRIAAP
                     IQLGTASLVVVLGIVLTGQALTQVL"
     gene            complement(9830..11203)
                     /locus_tag="VIF63_06265"
     CDS             complement(9830..11203)
                     /locus_tag="VIF63_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239019.1"
                     /translation="MTALTLPRPRRIRPVAMLVAAVGLVAATQLGAVVQAPPARPAQP
                     IVVSGPVTPPGAPVSATPADLGRIDKAIATWTANLTRNDKDFISAATLGMLYESRARL
                     SGDITDYTRAAEAATRSLAIEPRQLDVQALHARLLLATHDFAKALTEARAIDRAAPDQ
                     PAVLSIIADASLELGDIDLAAAQYARIEKIAPGAAVTARLARLSFLRGDPGGAVTQAE
                     AAYAAADAEGQTGPALSWYGYLAGTLTLAAGSPETAAGWFDKALAAWPGSYLALAGRA
                     RASAAIGDTARAIAGYRAAIAVAPQPDALTALGDLLALGGDTAGADDQYATVLAIARL
                     QGGDGLVYNRQLVLFDVNHGRDLATALTLAEQELAERHDAYGYDAYAWALLANGRAAE
                     ADAAMAKALAVGTHDALLLYHSAEIKLALGDHAAARQLLGDALAITGALDPLAAAKAT
                     ATLAGLR"
     gene            complement(11301..12488)
                     /locus_tag="VIF63_06270"
     CDS             complement(11301..12488)
                     /locus_tag="VIF63_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEY8239020.1"
                     /translation="MQRPSFKSGTLLAAAALTLALGGAPLLVTGADHLDAPALGGLTN
                     GLGEFAPHSDHGERDINDVYVFQGSNSSRTVLAMTTNPAVNAPTVQPMPNFGTDVRYI
                     INVDRNGNAVVDLAYVWRFGAAVGGSQPYTVTRYTGSNAPSLKTGVQIGKGWTGGTGI
                     GTAKGSAKVFAGVRSDPFFFDLTGFIGTVFGVGSDQLKVLDGTASDFFVGLNTNAVVI
                     EVPDDALGSTNIGVWGSTQWWNGSSWAKADQMGRPAINTVFNTFIVDGAAGAAKDLFN
                     TTSPSNQRTADGGRFRDNIIQTLTGINDYLGLDDLLGCEDWDAATAGVIADILLPDVI
                     TYDTTTPAVGPLNGRALADDVIDAELALTLNGCSVGNDPIGDDVGAHSDYQSTFPYLG
                     VPH"
     gene            12801..13337
                     /locus_tag="VIF63_06275"
     CDS             12801..13337
                     /locus_tag="VIF63_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8239021.1"
                     /translation="MDRLAGGDLGALDRLYEHYGAMSFSIAYRITGDRAAAEDVVQEA
                     FLGAWRNAARYADGRGTVRTWLLSIVHHRAIDAVRRRRPTAELPETETNLPGALTLPD
                     TWEEVAVGLDRAAIQVALSRISGVQREAIELAYFGGLTQTEIAERTGVPLGTVKGRLR
                     LGLDGLRAAMLATGVDRG"
     gene            13330..14226
                     /locus_tag="VIF63_06280"
     CDS             13330..14226
                     /locus_tag="VIF63_06280"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8239022.1"
                     /translation="MADHLIGGMHCADVLEDAPAFVLGALEASEMTAVRQHLAGCPEL
                     HAEFAELGSVVPALFEVVDLVEPSAMLRDRIVTAAAADTQRVADTQLAGDTERSIDTQ
                     RAADTQRAFERTPAFERTPVIDTQRDGGWLSNLFRRPVLPAAALAAALAIAALGAWNV
                     QLQNQIGGLTAYRDGVVAVLDQAAQPGAQLAVLAAPEGSGGPSGLAAVAADGSKVSIV
                     MRDLAPTTGAEVYEAWVISGDNAPVPIGHFTVDASGTATFATTGTPVTATAITVALTR
                     EPGPGATAPTMPIVALGTAEAS"
     gene            complement(14258..15274)
                     /locus_tag="VIF63_06285"
     CDS             complement(14258..15274)
                     /locus_tag="VIF63_06285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8239023.1"
                     /translation="MTPDPLLLAAIRAVARSGDPDATLDELLEVCLRAGGADRGAVFL
                     WDAERGGLALAGSRGYPPDDVPALEAQVATAEHPVQLAAQERIQTSGQSGDRTLSAWP
                     VVVGREGIEEPIGALALEGPSAPDADNSLRVAALADLVAVVVDRARVESHAEERLDWA
                     ERVFNSDPLTGLANAQTLTRVMELEIARAARQGAEVSVALFDVDDLTGFNDENGRLAG
                     DAVLREVAAVISETIRFVDTVARWGGDEFMLVAPGAKGTTVVRRIVEAVAAKAIGGGR
                     RRTVSAGLARFPADGTTSEALVGAAASALNAARAVGPGTLAEATQDANNGAGSGIETG
                     APGA"
     gene            15385..15714
                     /locus_tag="VIF63_06290"
     CDS             15385..15714
                     /locus_tag="VIF63_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEY8239024.1"
                     /translation="MKLVLAIIHNEDAGALVDGLLEKEYRATRLHSSGGFLKQSNATI
                     VLGVEDDRVDDVMSIIAEHCTSRTQVVNPMPPIMEPGEFFMPYPLEVEVGGATVFVVP
                     VERFERL"
     gene            15732..16328
                     /locus_tag="VIF63_06295"
     CDS             15732..16328
                     /locus_tag="VIF63_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8239025.1"
                     /translation="MTTWLDELEAGAPDAVGAYRNVEEGKFITVRREIRKRMSIGSTP
                     TVVPQLAAIADALGAMIDRLPDEAFTQPGGEGDWNVAQAIGHVASSRAGLSMAGALAA
                     SGRWPADAPPVVQGIPGAPTAGREELRRRIATSQRAIERAARSIEGHETDPCPLEHPT
                     VGRLRCGEWLFFAGVHDLMHLEQLHGIEAALADGARST"
     gene            complement(16325..17506)
                     /locus_tag="VIF63_06300"
     CDS             complement(16325..17506)
                     /locus_tag="VIF63_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8239026.1"
                     /translation="MIDLASVTVEELEADPYPIYAQLRTHAPVAYMPCLDTWMVTSWS
                     LVEAACTDPERFPAHVEHSPTNRALGGVSMMTTDGDPAKQRRRPFDPTLRPRAIEATM
                     PATFERLCDERLAAIAGAGSADLVPDYFEPVSVLSLAHVMGLGDAVDAPTLVRWFGGL
                     AAGVSNYEDDPAKTAHSAAISAEIDEATRPLFEARLADPDEGLISHLVHAADGSLDER
                     LAFILPSFKLVLLGGLQEPAHGGATITERLLRHPDQAGAMRADPALVPAAVEEGLRWT
                     APIGNLLRGVAPGTTFGGVAFPPDARAILVVAAANRDREAWGPTADEFDIHRPRRANL
                     SFAIGPHYCIGHFFARALLAVAVRRLFDRFPRLRLDPDHEPVYRGHEYRSPKTLRVLV
                     D"
BASE COUNT         2508 a         6141 c         6404 g         2582 t
ORIGIN      
        1 cgactcgatc ctgcgcggcg atgtccaatc gcccgagctg cgctggcgcg acgccgaggg
       61 cgtgcatcgc tcgcagtcgc ggccgcggcc gtcgccccgc gagcagctcg ctcccgatcg
      121 attctccggg ctcatcgggc agggcagctc gtggcggacc gagccgtcgt ggcgcggcga
      181 cggcgggttc cgcggcgact tccgtggcga ctaccgcgcc gacccgggcg gtcgcggcgg
      241 ctccggctct ggcttttttc ccggtgaacg tgggtcgcgg cccggattcc gcccgggtgc
      301 gtccggatcg tggcggcccg gccggtcgcc gtccggctcg ctgacggcag ccgccggcgc
      361 tggtgccggc gcgccgcggg tcgaggcgcc gttcgtcgcg ggcgagatcg cggatcgcgg
      421 gcctgtcgag cgcggcacga ccccggcgcc cgagccccgg gccagggagg cgcgcgagct
      481 gtcacgccag cgcgagtggc gcgagtcggc gacgaaggcc atcgacacgc tcaaggccga
      541 ggagggcacc ggcccggtca acgccgccga gctggtggct tcggagctgg ccgcgctgga
      601 ggctgccgct cgcaccgccg aattgggtct gcgcgcggcg ccgaccccgg gcatcgacga
      661 cctcgcggct gacgacggag cgctcctgga tggcgcgctt gatgacgacg atgacgacga
      721 cgatcgagcg tccggcatcc tcgagtcgga gcccgtgggc ctgccgaccc tgcgtctcct
      781 gccccagggc atcagccgga agcgcctcga gcaggcgatc cgcgagctcc agctgccggt
      841 gatcatcgcc cgtgacgtcg acgaggccga cgtcgtcatg acgatccgca gcgagtacaa
      901 gcagaagacg ccgatggttc gcgaggccga agagcgggcg atgccgatct acgtcctcaa
      961 ggccaacacg atcccccaga tgcaggcgtc gctgacctcg atcttcgccc tcgagatcga
     1021 cccgcgcgat gccgcgctgc gcgagaccga ggaggcgatc gggctggtca tcaaccgcgc
     1081 cgaggccatc gagctgtcac cgcagaacgc gtacatccgc cggctccagc accagatggc
     1141 cgagcgggcc aatctcgtct ctcgctcccg tggccgcgag ccctaccgcc gcgtccgcct
     1201 gtacccggac gccgcgaaaa gcccctggcg ctgacctgag ggcgcgccgg cgcacgccgt
     1261 cgccacaacc tgtgcggatt gccctagttg attgggcacc ggaactgagg atgggccgct
     1321 agaagtcctg acgctgccga catactggcg ttggatcggt caacccggtc ctgatgcaca
     1381 agttcgtggc gaatcgggag gcgatcgtgc aggagatcaa cggcggcgac acggcctgga
     1441 tgctcatcgc gaccgcgttg gtgatgatca tgctgcccgg gctcgcactg ttctacggcg
     1501 gcctggttcg ccgcaagaac gtcctgtcga cgatcatgca cagcttcttc gggctcgctc
     1561 tcgtcagcgt ggtctgggtc ctcgtcggct tttcgctcgc gttcgggaag gatgtgaccg
     1621 gcctgggctt gctcggcggc tcggagtatc tcgggttctc gaacgtcggc ctcgagccca
     1681 atccgacgta cggcccgacg atcccgttcg tgctgttcgc cgccttccag ctcatgttcg
     1741 ccgcgatcac gccggcgctc atcagcggcg cgttcgccga gcgcaagcgg ttcctcagct
     1801 tcgtgatgtt caccgggttg tggtcggtcc tcgtctactc gccgatcgcc cactgggtct
     1861 gggccaccga cggctggctg ttcaagctcg gcgccctcga cttcgccggc gggaccgtgg
     1921 tccatgcggc gtcgggcgtc gcggccctcg tcgcggctct cgccatcggc aagcgggcat
     1981 cgagcggcga caagatggag ccgcacgaca tcccgatgac cgtcctcggc gcgggactgc
     2041 tctggttcgg ctggttcggg ttcaatgccg gttcagcgct gactgccggc ggtcttgcgg
     2101 ccaatgccct cctcgtcacg aacacggcgg cggctgcggc gaccctcacc tgggcgttcg
     2161 ccagctacct ccatcgcggc aaggtgagcg tggttggcgc ggcgtgcggc gccgtcgctg
     2221 gtctcgtcgc gatcaccccc gcgtccgggt tcgtgacggc cggcggcgcg gtggtgatcg
     2281 ggctcgtggc gggcgggctg tgctacagcg cgacgctcct gcgcgagcgg ctgcggatcg
     2341 acgacgcgct cgacgtgttc gccgtccacg gcgtcggcgg cgtgttcggc gccatcgcga
     2401 ccggcgtctt cgcgaccacc gcgatcaacg cattcccggg cctggtcgat ggcaatcccg
     2461 gccagctcgt cacgcaggca atcgcggtcg gtgccacggt cgcctactca gcgggagcga
     2521 cattcgtgat catcaagctc gtcaacttcg tgctcgggct gcgcgtgccg gccgacgagg
     2581 aggccgtcgg cctcgatctc gcggtccacg gcgaggcggc gtaccagacc tgaccgtcgt
     2641 cgggtcgcta ctggcgctcc gccaccggct ccgcgctcgt cgccaaccgg gcaaggatcc
     2701 gctccgcgtc gctgacggtt cggtggacga tctgcgcggc gggcaggacc tcgtgcacga
     2761 gactgacacc ctggcccgcc cacagcgaca ggccctcgac gtcgccggtt gtccccatcc
     2821 gcaccgcgac cgaccagtag cgagggatct cgctgccgtc ggcgcggacg gcgatgacct
     2881 cgccctcgcc cgggcgctgg cccgacggcg gccggcccgc ggcctcccag gcgtcgatgg
     2941 tcgagttgcg cagcgtgcgg tgcgccgcgt tcggccagcc accgtcgaag agcatggagt
     3001 ggtaggtgct tgtctcgctc gcgtcgagga gccgggcaac gtagtccggg tgggccggtg
     3061 cctcctcgct cgccacgaac cgcgtgccca tccaggccgc accagcaccc agcgccagca
     3121 ccgccgcgag tccgcgaccg tcggtgatgc caccggccgc gatgaccggc gtcgcgccca
     3181 cctcgtcgat cactgcagga atgagggcga gcgtcgacac ctcgccaagg acgtgcccgc
     3241 ccgcctccca gccctgggcg acgatcacgt cgacacccgc gtcgacggcg gcgcgcgcct
     3301 gggccgcgct gccgacggtc agcatcgcga tcgccccggc atcgtggacc gcggcaatgt
     3361 agggcgccgg gtcgccccag aagaacgaga tgattttgac gccgttctcg agggcgacgg
     3421 cgagccgctc ttcctgcggc tcgtcgatga tcaggttgat cccgaacggc tgccccgtga
     3481 gggcctgcgt ggaccggatg gcctggcgca tctcgtccag ttcggtccag gtcatcgcga
     3541 tcaggccaag cccgccggcg ttcgacacgg ctgcggcgag acgggggttc gacaggccgc
     3601 cgatgggcgc ctggacgatc ggctgctcga tgccgagttt ccgcgtcacc gatgtctgca
     3661 ttgcgccctc ctttgatttc gaattcgtgg ctcagccggc ggccagcgcc gcccgatcga
     3721 tgaagatctg ctcacccatg aaccgctgcg tggcgggatc ccagggaaac aggatggcca
     3781 gctcgaggct gacgcgtcgg cccgtcggag cgaggccggc gaacgcctcg acgtgggtcg
     3841 cctcgaggac gaccgcttcg aacacgccct gcggcccgat gacgatgtcc gtcagcgaga
     3901 actggttgtc cggaaacgcg gcgaagagct ccgtgtagaa cgctcgggcg ccggcgtggc
     3961 ccgcccagcg cagcccggta ccgacgatct cgtagacaca ctcgggagcg agggtcgcga
     4021 tcagcccgtc gatgtcgcgc tggtcctcgg cgatcgagtg gcgctgccac agccgccgga
     4081 ttcgccgatg cgtgacggcg tcggcgggcc acccgagccg cgcccgaacc gactcccgaa
     4141 tctcgcctga tcccacgacc gaatcgtaag cgtcaaagga cgaggatgcc gctgtcgcgc
     4201 ggcgcgcgtc ctgccctcag ctccggcagg agttgccggc catcgagggg aaacctggtg
     4261 cggagggcct cccgatgcag atcaaggaag gtgcggttgt gcgccgtgtc gctgacgagg
     4321 aggatcatca actccacgtg gccatcgcgc atcttgagtg cgtaccggcg ctcgagcgac
     4381 tgagcgtcac gcagtcgggt ctccgcctcg atcgcgatcc ggatgcgatc gaagaccaga
     4441 agcccatccc acgcccgaag gtcgccggcg ccgggcggtt tgcgctgacc cgtatccgtc
     4501 catggtcgcc tggagggctc ctgggcctcg gacatccgct gcttggaacg cagcgcgtat
     4561 tccgactcgc ggttcaccgc ctggatggcg cgccccggcg gcactctcga gggtgaaatc
     4621 cgccgcagcg aacgctgggg gttccaaggc cggtgaggga ggtcggccgg acggagatgc
     4681 gcgacgggat tccggctaga actccgtgac gccggtgaag gtccagccgt caatgcgcag
     4741 ggcgggtacg acggcggcgc cgaggaagcc gcggatggtc ttgcggctgt caccgaccgc
     4801 ggtcacgccc gcgagggcat cgaggtagga ctgcgtgtag cgcaggttgc gcacagggcc
     4861 aacgagcttg ccgccctcga ccaggaacgt gccgtcgcgg gtcatgccgg tgacgatcgc
     4921 cagcttcggg tggacgacgt tcgtgtagtg gaagcgggtg acgagcaggc cgcgatcgag
     4981 gccgccgatc agctcgtcgc gcggcgtgga ccctgccgcc atcaccgcgt tcaagggaaa
     5041 cggcccgtac gggttcggcg ccggcagccc gtggccggtc gacgtgcggc cggcccgggc
     5101 ggccgtctgc gagtcgaaga ccacctcgcg gcacaccccg ccctcgacga gcggcacccg
     5161 ctgcttgggc acgccctcgt agtcgaaggc catcggcagg ccgttctggt ccgacccgtc
     5221 gtcgacgatg gtcacgaggt cggacccgat ccgccggccg ggctctgcga acgagcggcc
     5281 ctcctggacg gcaagcgcgg agaagccgac gtagccgagc tggtcggtga tgtcgacgac
     5341 ggcgtactcc tcgaggacga cgggatagtc gcccgcgggg acgctgaccg ggttgtcgct
     5401 ggcgcgcgcc ttcgcggccg cctcgcgccc gatcgccgcc gcgtcgatcg tggtcgcgtc
     5461 catgctcacg gcctcggcgt agccagtgcc gccgttcggc gacatgtgga ccgtcagcag
     5521 ctgcgacgag gtccggctct cggccgcgcg gatgcccgcc gagttcgcga cggccacgcc
     5581 ctcggagtcc gtcgcaaacg agccatacgc catgacgccc gccgcatccg cggcagcgat
     5641 gaccgcgcgg acgccctcgg cgcggaattc cggcgtcgcg tcggcggtgc ccgacgacca
     5701 gccgccgccc gttccgcgga cgaccgcgcc acccggggtc agcggcgcgg gcagtccggc
     5761 ccagtcctcg agctcctcgc agcttcgcgc gatcgcggcc gcccgatgga ccagtgatcg
     5821 cagcccgtcg gcgtcgacct tgccggtcga caccaccgcg atccggcgcc cgacgacgtg
     5881 ccggaggctc acggtgaggc tgcgctcggc cacgttctgg tggatctcgc tgttcgcgaa
     5941 gcgggtcagc gccgaatcgc ccgccaggac gagcacctcg gcctcggtcg cgccgacgcg
     6001 gagggcgcgc tcgagcacgt cctcggcaag ggccagcgcg gtcgccggat cccgggaatc
     6061 gacccgtccg gcgaggctcg cgtcgctcac catttgccca ccccgacctg gacgttgcgg
     6121 aagcgcgcgc cggacacggc gtggccaacc cgcgccgact gtgggggctc gcccttgccg
     6181 cagttcggcg tgccgagcat cacgaagctg ctctggtcgc caaccgcgtc gcacgagcgc
     6241 cagaactcgt acgtgatccc ggtgtacgtg cagttcttga acagccggcc cttcttgccg
     6301 cccttgatct cgtaggcgac ctcggtcgcg aactggaagt tcagccgtcg gtcgtcgatc
     6361 gaccagctcc ggttgttcgc gagatacagg ccgtcgtccg tatcggcgac gatctcatcg
     6421 aggctcatgc cgggtacggg cagcaggttc acattggtca tccggatcag cgggatccgg
     6481 ttccagccgt cggcgcgcat ctggccaccc gacatccggc cgacccgcgg cgccgtctcg
     6541 cggctggact ggtagccgac gagccgcccg ttctggatca acggcgtgga ttgcgctgcc
     6601 acaccctcgt cgtcccagcc gaacgtcccg agcccaccct cggcggtcgc gtcggcgacg
     6661 atggtcacga gctccgagcc gtactggaag ccttcctcga gcttgtccgg ggtgaggaag
     6721 ctcgtcccgg cgtaggacgc ctcggtgcca aagacgcgat cgagctcggt cgggtggccg
     6781 cagctctcgt ggatctggag gtacagcatc gaggggtcga ggacgagcgt gcgcatgccc
     6841 ggcgggcact gcggcgcgga cagcagggcg accgcctcct cgccgacccg tgtcgcgttg
     6901 ccctggagat cgagactgcg gatgtagtcg tagccgcccg cctggaagcc accgcccgag
     6961 tccgggtagc tccggcgctg gtgctcgtcg ccctcgatcg cgttggcctc gaccgcagcg
     7021 ccgacctgcg tgatgacctg ctcggtgaag ctgccgtcgg tcgccgcgaa ggtcttccac
     7081 tcgcggtggc ccgagtacat cgactcggtg aacgtcacgc ccttgacctt gcccgccgcg
     7141 agatccgcct cgacgaggtc ggagatcttg cgttccagcg ggatcttgaa cgggtcctcg
     7201 ccgacgggcg tttcgaaggt ccccgtggcg ggtggccggc catcgagctc gaccgggcgg
     7261 cgaagggcgg tcgacgacgc cttggcgatc cgcacggcca gcgcgccgac gcgatcggcc
     7321 tcggcgaggt cgagcgtgtc cgaggcagcg aagccccacg cgccatcgac gacgacccga
     7381 acgccgaacc cgtcggtctc gcccagggca acgccttcga cccgtccgct cttgaccgag
     7441 atcgactcgt cccggcggcg aacaacgcgg acgtccgcgt aggtcgcgcc cagctgggcg
     7501 gccgtgtcga gggcgcggtc ggtcaggtcc ttcatcgggg tggccgagcc taccagcgtt
     7561 cggcgggcgg ggcgacggag ggcccataca ctcctgctgt gagtgggttg ttcatcacgc
     7621 tcgaggggcc ggagggcggg ggcaagaccg tcatcgcgaa gcgcctcgtc gaggagctga
     7681 cgcggcgcgg tcacgtcgtc cgctcgacgc gcgaacccgg cgggacgcgg ctcggcgagc
     7741 gggtccgcgg gatcgtcctc gagcagtcgg ccgacgatcg ggccgccgcc atcgaccggc
     7801 gggccgatgc ccttctcttc aatgccgctc gagcgcagct ggtcgccgag gtcatccggc
     7861 ccgcgctcga ccggggcgag gtcgtgctct gcgcgcgcta cgccgactcg accctcgcct
     7921 atcagggcta cggcgccggc ctgccgctcg acgagctgcg cgcgctcgca gcggtcgcaa
     7981 ccggcgacct caagcctgat ctgacgatcc tgctcgacgt gccgcccgag atcgggatcg
     8041 ggcggaagac cggcgcgtcg cgcaatcgct tcgaatcgtc gttcgatctt gcgttccacg
     8101 agcgggtgcg cgcgggcttc ctcgcgctgg cgggccagga gccggaccgg ttccgggtca
     8161 tcgactcgtc gcgccgcatc ggcgccgtga tccccgaggt cgtcgccgcg gtcctggctg
     8221 ccctctgaga cctagaggac ctgggtcagc gcctggccgg tgaggacgat cccgaggacg
     8281 accacgagcg aggccgtccc gagctggatc ggcgccgcga ttcggcgcag gccacccatc
     8341 gagggtcgcc gctcgatcag ccggcgggcg ttgacgatga gcagcccggt cccggcgagc
     8401 accacggcca tgccgagccc gaagccaatg acgaggacga gcccgtaggc cacgcggccc
     8461 gcggcgatcg agccgagcag caggatcagc gccgacgctg atgggacgag gccgcccgac
     8521 agcccgagcg cgatgaggcc gcgccacgag atcgtggtgt cggtggcgac gtgcgagtgc
     8581 gggtgggggt gggggtgggg gtggccgtgc tccgggtcgg ggtcgtgcgc gtggtcagcc
     8641 gtgtgcgcgt gatcgtggcc gcgcgcgtcg tgcccatgct cgtgaccgtg gtccgtctga
     8701 tgcggtcgtc cgaggcccat cgcccggagg cgcgaccaca acagcgagcc gccgatcgcg
     8761 atgaccagtc cgcccgacac gacgccgagg atcggataga ggcgttcggg cgggaggacg
     8821 ttcgacgcgg cgagcgtgat cgcggccagc gcgaggacgc cgagggtgtg cgacacggcg
     8881 acggcgaggc cgagcccgaa cgcctggcgg ctcgagccct tcgcgccaac gaggtacgcg
     8941 gccatgatcg tcttgccgtg gcccggcgag agtgcgtgga cgatgcccag cgagaacgcg
     9001 atcgcgagtg acccgaggat tgccagcggc gtgaggtccg acacgtccac gagcgcagcg
     9061 aggtcctcgg cgatcccgcc cggcaccgcg ccgatcgggc ccggtccgga aggtgccggg
     9121 ggcgccgcaa gcggctgcgc gtccggcgcc aggaatgcgc cgagcgctgc gccgccgggt
     9181 gcaacgtcca ccgcgatgga gcgcatgtcg agcggttgcg tcagcaggtc tttcgggtag
     9241 gtcgtcagcc ggttcgagac accttcggcg ccggccggcg acgtttctgc acctgtcgcc
     9301 ccgatcgtga ccgtcgcgcc atcaccaagc accacgatct cgcgccagcc gatgcgctcg
     9361 gcgaacgatc gatcctcgaa gatgacggtc gacgaagcac cgatcggcga ggcaagggcc
     9421 gcttcgtact cacagacgag gcgcatcgtc ggcaggccgc cggcgccggg cgggagcgcc
     9481 aggcccgcag ccacgagcgt gggcgcgagc gcgcgaccgt ccacggcgag gcgcaggtcg
     9541 gaggcgaggg ttccgcatgc cacctggcgc gccgcttcga gctctgcggc agcgagcacg
     9601 ccgtcgccgt cgatgtcgag tcgctgctgc tcctggagcg ccgggatctc cgcgtggtcg
     9661 atgacgacgt cgagtgagat ccgatctggt gcgatccgga gcgcggcgta gtggttgatc
     9721 gtgaagttgc cgagcgggtg ggcgagtgcg atcgcaggca cgagcagcgc gaccacgacc
     9781 gcagccatcg cgccggcgat gcggcctggg cggggaacgc ggcggcggct catcgcaaac
     9841 ccgccagggt tgccgttgcc ttcgcggccg ccagcggatc gagtgcgccc gtgatcgcga
     9901 gggcgtcgcc gagcagctgc cgagccgccg cgtgatcgcc gagcgcgagc ttgatctcgg
     9961 ccgagtggta cagcagcagc gcgtcgtgcg tcccgacggc gagggccttc gccatcgcgg
    10021 catcggcttc ggccgcccgt ccattggcga gcagcgccca ggcgtaggcg tcgtagccat
    10081 acgcatcgtg gcgctcggcg agctcctgct cggcgagggt caacgcggtc gcgaggtcgc
    10141 ggccatggtt gacgtcgaac aggacgagct ggcggttgta cacgaggccg tcgccgccct
    10201 gcagacgggc gatcgcgagc acggtcgcgt actggtcgtc tgcgccggcg gtgtcgccgc
    10261 cgagggcgag caggtcgcca agtgccgtca gagcatccgg ctggggcgcg accgcgatgg
    10321 ccgcccggta cccggcgatc gcccgggcgg tgtcaccgat cgcagccgag gcgcgggccc
    10381 gaccggcgag cgcgaggtag ctgccgggcc atgcggcaag ggccttgtcg aaccacccgg
    10441 cagcggtctc gggtgagccg gcggcaagcg tgagcgtgcc ggcgaggtag ccgtaccagc
    10501 tcagcgccgg gccggtctgg ccctcggcgt cggccgcggc gtaggcggct tcggcctgcg
    10561 tcacggcccc accggggtcg ccgcggagga acgacaggcg ggcgaggcgc gcggtgaccg
    10621 ccgcacccgg ggcgatcttc tcgatgcgcg cgtactgcgc ggcggcgagg tcgatgtcgc
    10681 cgagctcgag gctcgcgtcg gcgatgatcg agaggacggc gggttggtcg ggcgctgcgc
    10741 gatcgatggc gcgcgcctcg gtcagggcct tcgcgaagtc gtgggtcgcc agcagtagac
    10801 gggcgtggag tgcctggacg tccagctgtc gtggctcgat cgccagggag cgcgtcgcgg
    10861 cctccgccgc gcgggtgtag tcggtgatat cgccgctcag gcgggccctg gattcgtaga
    10921 gcatgccgag cgttgcggcc gagatgaagt ccttgtcgtt gcgcgtgagg ttggcggtcc
    10981 aggtcgcgat ggccttgtcg attcgaccga ggtccgcggg cgtcgcgctc accggggcgc
    11041 cgggcggcgt caccgggccg gagacgacga tcggctgcgc gggtcgggca gggggtgcct
    11101 gcacgacggc cccgagctgc gtcgccgcga cgaggccgac cgcggcgacg agcatcgcca
    11161 cgggtcggat gcggcgcggt cggggcaggg tcagggcggt catccggtgc gctcctcgga
    11221 gatcagctga tgggtggcag aagggcggag cggaaggcgg ggagcgccgc ttgcgcgacg
    11281 ctccccgtat cccccgtggc tcagtgcggg acaccgaggt atgggaaggt cgactggtaa
    11341 tccgagtgcg cgcccacgtc atcgccgatc gggtcgttgc cgaccgagca gccgttgagc
    11401 gtgagagcga gctctgcgtc gatgacgtca tcggccaggg cacgcccgtt gagcggtccg
    11461 acagccggcg ttgtcgtgtc gtacgtgatg acgtcgggca gcaggatgtc ggcgatgaca
    11521 ccggcggtcg cagcgtccca gtcctcgcag ccgagcaggt cgtcgagccc gaggtagtcg
    11581 ttgattccgg tcagcgtctg gatgatgttg tcccggaacc ggccaccatc ggccgtccgc
    11641 tgattggacg gcgatgtcgt gttgaacagg tccttggcgg cgccggcggc gccgtcgacg
    11701 atgaaggtgt tgaacacggt gttgatggcc gggcgaccca tctggtcggc cttggcccac
    11761 gaggagccgt tccaccactg ggtggagccc cagacaccga tgttcgtcga gccgagcgcg
    11821 tcgtccggaa cctcgatgac gaccgcgttg gtgttgagcc caacgaagaa gtcagacgcg
    11881 gtgccgtcca ggaccttcag ctggtccgaa ccgacgccga agaccgtgcc gatgaagccg
    11941 gtcaggtcga agaagaacgg gtcgctccgg acgccggcga agaccttcgc gctgcccttt
    12001 gccgtcccga tccccgtgcc gccggtccag cccttgccga tctggacgcc cgtcttgagg
    12061 gacggggcat tgctgcccgt gtaccgggtg acggtgtacg gctggctgcc gccgacggcc
    12121 gcgccgaagc gccagacata ggcgagatcc acgaccgcgt tgccgttgcg gtcgacgttg
    12181 atgatgtagc gaacgtccgt gccgaagttc ggcatcggct gcaccgtcgg tgcgttgacc
    12241 gccgggttgg tggtcatcgc gagcacggtc cgcgacgagt tgctgccctg gaacacgtac
    12301 acgtcgttga tgtcgcgttc gccgtgatcc gagtgcggag cgaactcgcc gaggccgttg
    12361 gtcaggccac cgagtgccgg cgcatcgagg tggtccgcgc ccgtgacgag cagcggagcg
    12421 ccgcccagcg ccagggtgag ggcagctgcg gcaagcagcg tcccgctctt gaatgaaggc
    12481 cgttgcatcg agatcttccc ctccaaggcc aacccgaacc agcaccgggc cgacgcgtca
    12541 cgacacggat tgagtcaagg gttggtatgc gtcagacggg gcatctgcgc cgtcctcgcc
    12601 ggtgttacgc ggcaccgctg gtggcggatc actccgggat ttcttcgccg atcgcgtcgc
    12661 tacactcggg ccgtgctgac gggctgcatg gccgggaatg aaccgttgac gtccgccctg
    12721 cgaacagtcc gatgagcgag cccgagatcg cggcccgcgg cggacgggga cccgattcag
    12781 atcggaccga tcgggaaacc atggaccgtc tggcgggcgg cgatctcggc gcgctcgatc
    12841 gactgtacga gcactacgga gcgatgagct tttcgattgc ctaccgcatc accggcgaca
    12901 gagcggctgc cgaagacgtt gtccaggaag cattcctggg cgcctggcgc aacgctgccc
    12961 gatacgccga tggccgcggt acggtccgca cctggctcct gtcgatcgtc catcaccggg
    13021 cgatcgatgc ggtgcgtcgc cgtcggccga ccgccgaact ccccgagacc gagaccaacc
    13081 ttcccggcgc gctgaccctc ccggatacct gggaggaggt cgcggtcggc ctcgatcgcg
    13141 cggcaatcca ggtcgcgctg tcgcgcatct ccggcgtcca gcgcgaggcg atcgagctgg
    13201 cctacttcgg cggcctgacc cagacggaga tcgcggagcg aaccggcgtg ccgctcggga
    13261 cagtcaaggg tcggctccgg ctcggcctcg acggcctgcg tgcggccatg ctcgcgaccg
    13321 gggtggaccg tggctgatca cctgatcggc ggaatgcact gcgccgacgt gctcgaggac
    13381 gccccggcct tcgtgctggg tgcgctcgag gcgtccgaga tgacagccgt tcgtcagcac
    13441 ctcgcgggct gcccggagct gcatgcggag ttcgccgagc tgggctcggt cgtgcccgcg
    13501 ctgttcgagg tcgtcgacct cgtcgagcct tcggcgatgc tgcgcgaccg gatcgtgacc
    13561 gccgccgccg ccgacacgca gcgggtcgcg gacacgcagc tcgcgggcga cacggaacgg
    13621 tcgatcgaca cgcaacgcgc cgcggatacc caacgcgcat tcgaacgcac gccggccttc
    13681 gagcgcacgc ccgtcatcga cacccagcgc gatggcggct ggctctcgaa cctgttccgc
    13741 cggcccgtct tgcccgcggc cgccctcgcg gcggccctcg ccatcgccgc cctgggcgca
    13801 tggaacgtcc agctccagaa ccagatcggc ggactcaccg cgtaccgcga cggggtcgtc
    13861 gccgtgctcg atcaggcggc ccagcccggg gcgcaactcg ccgtgctcgc tgcgcccgag
    13921 gggtcaggtg gaccgtcggg actcgccgcc gtggccgccg acggctcgaa ggtgtcgatc
    13981 gtcatgcgcg atctcgcccc gaccaccggc gccgaggtct acgaggcctg ggtcatcagc
    14041 ggcgacaacg cgcccgtgcc gatcggccac ttcacggtcg atgcgagcgg gacggcgaca
    14101 tttgccacga ctggaacacc ggtgaccgcg accgcgatca ccgtcgcgct gacccgtgaa
    14161 cccggccccg gcgcgaccgc gccgacgatg ccgatcgtcg ccctggggac cgccgaagcg
    14221 agctgagcct cggagcgcct ccgagggcgc tcgaccgtca agcgccgggg gcgcccgtct
    14281 cgatcccgga acctgcgccg ttgttggcgt cttgcgttgc ttcggcgagc gtcccgggtc
    14341 ccactgcccg agcggcattc agtgcggagg cggcggcgcc aacgagcgct tcgctcgtcg
    14401 tgccgtcggc ggggaagcgc gccaggccgg ccgaaaccgt ccggcgccga ccgccgccga
    14461 tcgccttcgc cgcaaccgcc tcgacgatcc ggcgcaccac ggtcgtgcct ttcgcgccgg
    14521 gcgccacgag catgaactcg tcgccgcccc agcgcgccac ggtgtcgacg aagcggatgg
    14581 tctcgctgat caccgctgcg acctcgcgca gcaccgcgtc gcccgcgagt cggccgttct
    14641 cgtcgttgaa gcccgtcaga tcgtcgacgt cgaacagcgc gaccgacacc tcggcgccct
    14701 ggcgcgcggc gcgcgcgatc tcgagctcca tgacccgtgt cagcgtctgg gcgttggcga
    14761 ggccggtgag cggatcagag ttgaagacac gctcggccca gtcgaggcgc tcttcggcgt
    14821 ggctctcgac ccgcgcccga tcgacgacga ctgccacgag gtcggcgagc gcagcgacgc
    14881 gcagcgaatt gtcggcatcg ggcgcggacg gcccttccag ggcgagggcg ccgatcggct
    14941 cctcgatccc ttctcgcccg accacgaccg gccacgcgct caatgttctg tcgccgctct
    15001 ggcccgacgt ctggatccgt tcctgggccg cgagctggac cgggtgctcg gccgttgcga
    15061 cctgtgcctc cagcgccggg acgtcgtcgg gcggatagcc acgcgagccg gcgagggcga
    15121 ggccgccacg ctccgcatcc cacaggaaga cggcgcctcg atcggcaccg ccggcccgga
    15181 ggcagacctc gagcagctcg tcgagcgtcg cgtcggggtc gccactgcgg gcgacggcgc
    15241 ggatggcggc gaggagcaga gggtcgggtg tcacggcggg ccgatcgtac accggtcgag
    15301 acccccgtca atcgaacaag cggtcgcact tcgcaggcgg cctccgacca tggcgcggtt
    15361 ctgccccggc atatactccc ggccgtgaag ctcgtgctcg cgatcatcca caacgaggac
    15421 gccggcgcgc tcgtggatgg gttgctcgag aaggagtacc gcgcgacgcg gctccactcg
    15481 tcgggcgggt tcctgaagca gtccaacgcg acgatcgtgc tcggggtcga ggacgaccgc
    15541 gtcgacgacg tcatgtcgat catcgccgag cactgcacct cgcggaccca ggtcgtgaac
    15601 ccgatgccac cgatcatgga gccgggcgag ttcttcatgc cctacccgct ggaggtcgag
    15661 gtcggcgggg cgacggtgtt cgtcgtgccg gtcgaacgct tcgagcgact ctgacggaac
    15721 cgcgtgtccc ggtgacaacc tggctcgacg agctcgaagc cggagcgccc gacgccgtcg
    15781 gcgcgtaccg gaacgtcgag gagggaaagt tcatcacggt gcgccgcgag atccggaagc
    15841 ggatgtcgat cgggagcacg ccgaccgtcg tcccgcagct cgcggcgatc gccgatgccc
    15901 tcggggcgat gatcgaccgg ctgccggacg aggccttcac ccagccgggc ggcgaaggcg
    15961 actggaacgt cgcgcaggcg atcggccacg tcgcgtcatc ccgtgccggc ctgtcgatgg
    16021 ccggggcgct cgccgcgtcg ggtcgctggc cggcggatgc gccaccggtg gtgcagggga
    16081 tcccgggggc gccgaccgcc ggtcgcgagg agctgcgccg ccggatcgcc acgagccagc
    16141 gggcgatcga gcgcgccgcc cgttcgatcg agggccacga gaccgatcca tgccccctcg
    16201 agcacccgac cgtcgggcgg ctccgctgcg gcgagtggct gttcttcgcc ggggtccatg
    16261 acctcatgca tctcgagcag ctgcacggga tcgaggccgc tctcgcggac ggagcgcgat
    16321 cgacctagtc gacgagcacc cggagcgtct tcggcgaccg gtactcgtgg ccgcggtaga
    16381 cgggttcgtg gtccggatcg aggcggaggc gcgggaagcg gtcgaacagg cgccggaccg
    16441 cgacggccag cagggcccgg gcgaagaagt ggccgatgca gtagtgcggg ccgatggcaa
    16501 acgacaggtt cgcccgtcgc ggccgatgga tgtcgaactc gtcggcggtc ggcccccagg
    16561 cctcgcgatc gcggttcgcc gcggcgacga cgaggatcgc gcgggcgtcc ggcgggaacg
    16621 cgacgccacc gaacgtcgtg cccggcgcaa cgccgcgcag gaggttcccg atcggcgcgg
    16681 tccaccgcag gccctcctcg accgcggccg gcacgagcgc cggatcggcg cgcatcgcgc
    16741 cagcctgatc cgggtgtcgg agcagccgct cggtgatcgt cgcgccacca tgcgcaggct
    16801 cctggaggcc gcccaggagc acgagcttga acgacgggag gatgaacgcg agccgctcgt
    16861 cgagcgagcc gtcggcagcg tggacgaggt ggctgatcag gccctcgtcg ggatcggcca
    16921 ggcgggcctc gaacaacggc cgggttgcct cgtcgatctc ggccgagatc gcggcggaat
    16981 gcgcggtctt ggcggggtca tcctcgtagt tggagacgcc ggcggcgagg ccgccgaacc
    17041 agcgcacgag cgttggcgcg tcgacggcgt cgccgaggcc catgacgtgg gcgagcgaca
    17101 ggacggagac cggctcgaag tagtcgggga cgaggtccgc cgagccggcg ccggcgatcg
    17161 cggcgagccg ctcgtcgcac agccgctcga acgtggccgg catcgtcgcc tcgatcgccc
    17221 gcggccgaag cgtcgggtcg aacggccggc gccgctgctt ggccgggtcg ccgtcggtgg
    17281 tcatcatcga gactccgcca agcgcccgat tcgtcggcga gtgctcgacg tgggccggga
    17341 accgctccgg atccgtgcac gctgcctcga ccagcgacca gctcgtgacc atccacgtgt
    17401 cgaggcaggg catgtacgcg actggagcgt gggtccgcag ctgcgcgtag atcggatatg
    17461 ggtccgcctc gagctcctcg acggtcaccg acgcaaggtc gatcacagcg cgacctcgag
    17521 gtgctcgccc tcgatgcgca cggggatcga ccggaccgga cgctcggccg gcccgcgctt
    17581 cagcgcgcct gttcgcaggt cgaactcgga gccgtggcag ttgcagacga tgtcg
//
LOCUS       DATBSW010000224         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_620524, whole genome shotgun sequence.
ACCESSION   DATBSW010000224 DATBSW010000000
VERSION     DATBSW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620524"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VIF63_06305"
     CDS             complement(<1..560)
                     /locus_tag="VIF63_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239027.1"
                     /translation="MIRCTKCQTINPPDAIFCSNCNSFLEWTGVKVDPAELAAEASAK
                     EAAEKAAAEAAAQETAAKEAEAMAAREAADREAAAVAAREAEAKAAAEREAAEKEAAE
                     KEAAEKAKAEAKAAAEREAEAKATAERDAAEQAAAEKVAAEKAAAEKAEAEAKATAER
                     DAAAKAAAKKAEREAAKSAADSDAATA"
     gene            complement(632..1180)
                     /locus_tag="VIF63_06310"
     CDS             complement(632..1180)
                     /locus_tag="VIF63_06310"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY8239028.1"
                     /translation="MRGLVEALESPRPLIDSLPGIYQEDDLARAVTGVFDDGLAPIMS
                     TIDNVAAYLDPALTPDDFLEWLSGWVGILPDETWPIERRRAVIALATQLYRKRGTAAG
                     LAMHIKLLGASDVEVKDSGGASWSKTAGSKAPGDASYSLKVNVTPPKKGKLDPARIEA
                     LVATAKPAHIAHEVKIRDGSAA"
     gene            complement(1177..3126)
                     /locus_tag="VIF63_06315"
     CDS             complement(1177..3126)
                     /locus_tag="VIF63_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY8239029.1"
                     /translation="MPLLAPNLDDRRFQDLVDDAKRLVQERTDRWTDHNVSDPGVTLI
                     ESFAWMTDQLLYRLNRVPDRNYVKFLELIGVHLYPPAAAHAPVTFWLSAPQPSTVTIP
                     AGTEVATVRTGEQQPVVFSTTEDRPIITASLARMGSMVDGKTLRDHGDALSMKTGVYP
                     FSSPPKPGDALLVGLTAAVPGCVVNLRFTARIEGVGVDPDDPPLAWEAWTGEDWAACE
                     LERDTTGGINRDGDVVLHVPAGHIEHTLEKVKAGWLRARITEPHERQPAYSASPEIRG
                     LSAYTIGGTADAINAELVRNEYLGESEGVPGGRFTLKKAPVVAGEKPLILETLEGDAW
                     QEWTQRPNFAASGPEDRHFVLDPSTGTIELGPAVRLADGSQRRYGAVPARGAGIRVPL
                     YRAGGGQAGNVSVGALSVLKASIPYVARTENRRAARGGVDGEDIEQAKVRGPIVLRTR
                     DRAVTGEDFEHIAREVAPEVARVKALATAEGSETGVVRVLIVPAVPGKDGRLAFDQLV
                     PSEETLKRITARLDETRLVGTRVVVEPPAYRGVTVVARVVARQRTDPARVQSDALDAL
                     FRYFHPTMGGADGTGWPFGRPILAGEVFAALQAVRGTEIVEDVRIFGADPLTGERGKP
                     TDRLDVEPNALVFSYDHQVLVEAQK"
     gene            complement(3145..3543)
                     /locus_tag="VIF63_06320"
     CDS             complement(3145..3543)
                     /locus_tag="VIF63_06320"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY8239030.1"
                     /translation="MAHEFVGAGWAFPLAVDATGGFELVTDEREVEQAIRLIIGTAYG
                     ERPMRPDFGCRIHDFVFAEANAATAGQIANEVRASLARWEPRIVVDEIVVNIDDDDHS
                     LLYVDIRYHIRNRNDPRNLVFPFYTIPPER"
     gene            complement(3553..3933)
                     /locus_tag="VIF63_06325"
     CDS             complement(3553..3933)
                     /locus_tag="VIF63_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY8239031.1"
                     /translation="MGNPALVTGDKVNGTCPAHQIPSASGTAPAGPLPFGAPITQGTI
                     ATVLIGGKPAAVMGSSGYNTPPHAGIVDPPFASPTSQVGRIISGSPTVLIGGKMAATT
                     MSKATMCVAPATACGPGVATVLIG"
BASE COUNT          510 a         1464 c         1461 g          683 t
ORIGIN      
        1 gctgtcgctg cgtcggaatc cgccgcggac ttcgccgcct cgcgttcggc cttcttcgcg
       61 gccgctttcg cggctgcatc gcgctccgcg gtggctttcg cctcggcctc ggccttttcc
      121 gcagccgcct tttccgcagc caccttttct gccgccgcct gctcggctgc gtcgcgctcc
      181 gcggtggctt tcgcctcggc ctcgcgctcc gcagcggctt tcgcctcggc cttggccttt
      241 tccgcggcct ccttctcggc ggcctccttc tcggcggcct cgcgctcggc ggcggctttc
      301 gcctcggcct cgcgcgccgc cacggccgca gcttcccggt cggcggcttc gcgcgccgcc
      361 atcgcctcgg cctccttcgc cgcagtttcc tgtgccgcag cctcggcggc ggccttctcg
      421 gccgcttcct tggcggaagc ttccgcggcg agctcggccg gatcgacctt gacgccggtc
      481 cactcgagga agctgttgca gttcgagcag aagatcgcgt cgggagggtt gatcgtctgg
      541 cacttggtgc agcggatcat ccggcgggtg acctcaggat cgggacgctg gtcgtggacg
      601 ctggtctgga cgggggcgcg gccgcccgat cttacgcggc cgagccgtcc ctgatcttga
      661 cctcgtgggc gatgtgcgca ggtttcgccg tcgcgacgag ggcctcgatc ctcgccgggt
      721 cgagcttgcc cttcttcggc ggcgtcacgt tgaccttcag cgagtacgag gcgtcacccg
      781 gcgccttcga gccggccgtc ttcgaccacg acgcgccgcc gctgtccttc acctcgacgt
      841 cgctcgctcc gagcagcttg atgtgcatcg cgagccccgc cgccgtcccg cgctttcgat
      901 acagctgcgt ggccagggcg atgaccgcac ggcggcgttc gatcggccag gtctcgtcgg
      961 gcaggatgcc gacccagccg gacagccact cgaggaagtc gtcgggcgtg agcgccgggt
     1021 cgaggtaggc ggcgacgttg tcgatcgtgc tcatgatcgg cgcgaggccg tcgtcgaaca
     1081 ccccggtcac ggcccgcgcg aggtcgtcct cctggtagat gcccggcagc gagtcgatca
     1141 gcggcctggg cgactcgagc gcctcgacca gccccctcac ttctgcgcct cgacgagcac
     1201 ctgatggtcg tacgagaaga cgagcgcgtt gggctcgacg tcgagccgat ccgtcggctt
     1261 gccgcgctcg cccgtgagcg ggtccgcacc gaagatccgg acgtcctcga cgatctcggt
     1321 cccgcggaca gcctggagcg ccgcgaacac ctcgcccgcg aggatcgggc ggccgaacgg
     1381 ccagccggtg ccatccgcgc cgcccatcgt cgggtggaag tagcggaaca gggcgtcgag
     1441 cgcgtccgac tggacccgcg ccggatcggt ccgctggcgg gcgacgacac gcgcgacgac
     1501 ggtgacgcca cggtacgccg gcggctcgac gacaacgcgc gtgccgacga ggcgggtctc
     1561 gtcgaggcgg gcggtgatcc gcttgagcgt ctcctcgctc ggcaccagct ggtcaaatgc
     1621 cagccggcca tccttgccgg gcacggcggg gacgatcagg acacggacaa cgccggtttc
     1681 cgacccctcc gcggtcgcga gcgccttgac ccgggcgacc tcgggcgcga cctcgcgcgc
     1741 gatgtgctcg aagtcctcgc cggtcacggc gcgatcgcgg gttcgcagca cgatcgggcc
     1801 gcgaaccttg gcctgctcga tgtcctcgcc gtccacgccg ccgcgagcag cgcgccggtt
     1861 ctcggtccgc gccacgtacg ggatcgacgc cttgagcacg gacagcgccc ccacggagac
     1921 gttgcccgcc tggccgccac cggcccggta caagggcacg cggattccgg cgccgcgggc
     1981 gggcacggcg ccgtagcgac gctgcgagcc gtcggcgagt cgaacggcgg gcccaagctc
     2041 gatggtcccg gtcgaggggt ccagcacgaa gtgccggtcc tcgggtccgc tggcggcgaa
     2101 gttcggacgc tgcgtccact cctgccaggc atcgccctcg agcgtctcga ggatcagcgg
     2161 cttctcgccg gcgacgaccg gcgccttctt gagcgtgaac cgaccgccgg gcacgccctc
     2221 ggactcgccg aggtactcgt tgcggacgag ctcggcgttg atcgcgtcgg cggtgccgcc
     2281 gatcgtgtac gcagagaggc cgcggatctc gggcgacgcg ctgtaggcgg gctgccgctc
     2341 gtgcggctcg gtgatgcggg cccgcagcca gcccgccttg accttctcga gcgtgtgctc
     2401 gatgtggccg gcaggcacgt ggagcacgac atcgccgtcg cggttgatgc cgcccgtggt
     2461 gtcgcgctcg agctcgcagg ccgcccagtc ctcgccggtc caggcctccc aggcgagcgg
     2521 cggatcgtcc gggtccacgc cgacgccttc gatgcgggcg gtgaaccgga ggttgaccac
     2581 gcagcccggt acggcggctg tcaggccgac gagcaacgcg tcccccggct tcggcggact
     2641 cgagaacggg tacacgccgg tcttcatgga cagcgcgtcg ccgtggtcgc ggagggtctt
     2701 gccatcgacc atcgagccca tccgcgccag cgacgccgtg atgatcgggc ggtcctcggt
     2761 ggtgctgaac acgacgggct gctgctcgcc cgtgcgcacc gtcgcgacct cggtgccggc
     2821 cgggatcgtg accgtcgacg gctggggcgc ggacagccag aacgtgaccg gcgcgtgggc
     2881 cgccgccggc ggatacaggt ggaccccgat cagctcgagg aacttcacgt agttgcggtc
     2941 cgggacgcgg ttgagccggt acagcagctg gtcggtcatc caggcgaacg attcgatgag
     3001 ggtcacgccc ggatcggaca cgttgtggtc cgtccagcgg tcggtccgct cctggacgag
     3061 gcgcttggcg tcgtcgacca ggtcctggaa ccggcgatcg tcgaggttcg gtgcgagcag
     3121 gggcatggcg gtcgactcct ccggtcagcg ctctggcggg atcgtgtaga acgggaacac
     3181 gaggttgcgt ggatcgttcc gattccggat gtggtagcgg atgtccacgt acagcaggct
     3241 gtggtcgtcg tcgtcgatgt tcacgacgat ctcgtcgacg acgatccgtg gctcccaccg
     3301 tgccagcgag gcgcggacct cgttcgcgat ctgtccggcg gtggcggcgt tggcctccgc
     3361 gaacacgaag tcgtggatcc ggcagccgaa gtcggggcgc atcgggcgct cgccgtaggc
     3421 cgtgccgatg atcaggcgga tcgcctgctc gacttcgcgc tcgtccgtga ccagctcgaa
     3481 gccgcccgtc gcgtccaccg cgagcgggaa tgcccaaccg gcgccgacga actcgtgggc
     3541 catgtcgctc ccctacccga tcagcacggt ggcgacgccg ggaccgcacg ccgtcgcggg
     3601 ggcgacgcac atcgttgcct tggacatggt cgtcgccgcc atctttccgc cgatgagcac
     3661 ggttgggctg ccgctgatga tccggccgac ctggctcgtc ggtgacgcga acggcgggtc
     3721 gacgatgccg gcatgcggcg gcgtgttgta gccggacgag cccatgaccg cggcgggctt
     3781 gccgccgatc aggacggtcg cgatggtgcc ctgcgtgatc ggcgcgccga acggcagcgg
     3841 ccccgccggc gcggtgccgg acgcggacgg gatctggtgc gccgggcagg tgccgttgac
     3901 cttgtcgccg gtcacgagcg ctggattgcc cacggccccg ctcctagttg atcttcacgg
     3961 tggcgccctt gaggacgagc tcgccggtcg cctgcacggt gaccttcgcc cccttgagcg
     4021 tgatgtcgcc cacggcatcg acgtcgacgt cgcccatgct ctggacgacg agcttcttgt
     4081 ccttgccgtt cagcccgatg gtcacggcgg actccttg
//
LOCUS       DATBSW010000225         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_938129, whole genome shotgun sequence.
ACCESSION   DATBSW010000225 DATBSW010000000
VERSION     DATBSW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938129"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VIF63_06330"
     CDS             complement(<1..433)
                     /locus_tag="VIF63_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239032.1"
                     /translation="MSLDLDAATTAISAADPMFGAFIAREGPFPPRPGQGDPLSSLAR
                     AIVFQQLATRAAEAIHRRFVAAIGGTVTAEAILATELTDLRGAGLSAAKATSLIDLAT
                     KSIDGTVLLDGLAALDDDEIVARLIQVRGIGRWTAEMFLLFE"
     gene            complement(479..1720)
                     /locus_tag="VIF63_06335"
     CDS             complement(479..1720)
                     /locus_tag="VIF63_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8239033.1"
                     /translation="MDRNRGLQHRLTATGAGLALVFAATLAAPVAAQPLPVVEARDAA
                     IVSQAAPTDAHIQLVLRASNLSNPVFVTSARDGTGRLFIVEQTGKIKIYVNGAVQSTP
                     FLSIGSQVSKGYEQGLLGLAFHPNFTSNRKLYVNFTDVNGDTVVREYKASSTNPNVVS
                     TSTARTILKVYQPYDNHNGGMLAFGPDGYLYIGMGDGGDGDDPGNRAQNKDTLLGKIL
                     RININGSSSAHNYLFPSSNPYVGVFGRNEIWQIGTRNPWRFSFDRANGNLWIGDVGQE
                     QYEEVDRAVKTSSGAGRGVNWGWRVLEGFHCHIPSTGCSTSGKTLPLAEYPHSGSRCA
                     MTGGYVYRGTQIPALVGGYVFADYCSGEIWVLNSTATSPANEVLLLDTSLTISSFGEN
                     AAGELYVMDREGGKMYAILGA"
     gene            1924..>2781
                     /locus_tag="VIF63_06340"
     CDS             1924..>2781
                     /locus_tag="VIF63_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY8239034.1"
                     /translation="MTPTRIANPRVDEHGATFSWHGAGPPPVVAGSWCGWDPARGLEM
                     ERVGGGTWEARLEMQADAYAEYRLYRSDRVVADPGNRDRIDNGFHGRNQRFWMPRATR
                     RAEQLIRRDVPRGHVTRAKVDLGWLSAPPSRRRVAFYLPDVAAHDTEIRRSLPLLVVL
                     DGLDYLRRGGLAEMLDALIADGSIAPVAALFVDNAGPGRPSEYMANDFTLASLADICV
                     PAAVEELGLETQHAAVGPGRAAILGSSLGGLMALHAGVRRPDVFGRVIAQSTSAMLDE
                     LPLRDSVIPA"
BASE COUNT          401 a          935 c          998 g          447 t
ORIGIN      
        1 gctcgaacag caggaacatc tcggccgtcc agcggccgat gccgcgcacc tggatgaggc
       61 gggcgacgat ctcgtcgtca tcgagcgcgg cgagtccgtc gagcagaacg gtgccgtcga
      121 tcgacttcgt cgccaggtcg atcagggacg tggccttggc ggccgagagc cccgcgcctc
      181 gcaggtcggt cagctcggtg gcgagaattg cctcggccgt caccgtgccg ccgatcgccg
      241 ccacgaaccg gcgatggatc gcctcggcag cccgcgtcgc cagctgctgg aacacgatcg
      301 ctcgcgccag cgaggagagc ggatcgccct ggccgggtcg cggcgggaag ggaccttcgc
      361 gggcgatgaa cgcgccgaac atgggatcgg cagcggagat cgcggtcgtc gccgcgtcga
      421 ggtcgaggga catccctcgc aggatggcag agcttcggcg gtcgggcggc tcgtgcggtc
      481 aggcgcccag gatcgcgtac atcttcccgc cctcgcggtc catgacgtac agctcgccgg
      541 ccgcgttctc gccgaacgac gagatcgtga ggctcgtgtc gagcagcagc acctcgttcg
      601 ccggtgaggt ggcggtcgag ttgagcaccc agatctcgcc ggagcagtag tcggcgaaga
      661 cgtagccacc gaccagcgcc gggatctgcg tgccgcggta cacgtagccg ccggtcatcg
      721 cacagcgact gccgctgtgg gggtattccg ccagcggcag cgtcttgccc gacgtgctgc
      781 agcccgtcga ggggatgtgg cagtggaacc cttcgagcac gcgccagccc cagttcacgc
      841 cccggcccgc acccgaggac gtcttgacgg cgcgatcgac ctcctcgtat tgctcctggc
      901 cgacgtcgcc gatccacagg ttgccgttag cccgatcaaa cgagaatcgc cacgggttgc
      961 gcgtgccgat ctgccagatc tcgttgcggc cgaagacccc gacgtacggg ttggacgacg
     1021 ggaacaggta gttgtgggcc gacgacgagc cgttgatgtt gatccgcagg atcttgccga
     1081 ggagcgtgtc cttgttctgg gcccggttgc ccgggtcgtc gccgtcgccg ccgtcgccca
     1141 tgccgatgta caggtagccg tcaggtccga aggcaagcat gccgccgttg tggttgtcgt
     1201 acggctggta gaccttgagg atcgtgcgcg ctgtgctcgt cgagacgacg ttggggttcg
     1261 tgctcgaggc cttgtactcg cggacgacgg tgtcgccgtt gacgtccgtg aagttgacgt
     1321 acagcttccg gttcgacgtg aagttcgggt ggaacgccag gcctagcagg ccctgctcgt
     1381 agcccttgct gacctgcgag ccgatcgaca ggaagggcgt cgattgcacc gcgccgttca
     1441 cgtagatctt gatcttgccg gtctgctcga cgatgaacag tcgtcccgtc ccatcgcggg
     1501 cgctcgtcac gaacacgggg ttcgacaggt tcgacgcccg aagcaccagc tggatgtggg
     1561 cgtcggtggg cgccgcctgg ctcacgatgg cggcgtcacg ggcctcgacg acgggcagtg
     1621 gctgggcggc gacgggcgcg gcgagggtgg ccgcgaagac cagcgcgagg ccggcgccgg
     1681 tcgcggtcaa tcgatgctgg agcccccggt ttcgatccat gacccggcca tcctgtcgcc
     1741 ggaccgtccg gcctgtccat aggagtctcg tcacgtgccg gtcaacgtgc ggcccgggtc
     1801 gccgggcatg agcaccgtcg gggcgacgtc gccctcgtcg atcgcgaggt ggtactgggt
     1861 catccgcccc gacagtagca gccgcaagaa ccaccgaccg tcgcccgtat gcaaggatgc
     1921 gacgtgaccc ccacccggat cgccaaccct cgcgtcgacg agcatggcgc gacgttctcc
     1981 tggcatggcg ccggaccgcc gccggtcgtc gccgggtcgt ggtgcggctg ggaccctgcg
     2041 cggggtctcg agatggagcg cgtcgggggc ggcacgtggg aggcgcggct cgagatgcag
     2101 gctgacgcgt atgccgagta ccgcctgtac cggtcggatc gagtcgtggc cgatccgggc
     2161 aaccgcgacc ggatcgacaa cgggttccac ggccggaacc agcgcttctg gatgccgcga
     2221 gcaacgcgcc gcgccgagca gctgatccgg cgggacgtgc cgcgcggcca cgtgacccgg
     2281 gcgaaggtgg acctgggctg gctgtcggcc ccgccgtcgc ggcgccgggt cgcgttctac
     2341 cttcccgacg tcgccgcgca cgacaccgag attcgacgat cgttgccatt gctcgtcgtc
     2401 ctcgacgggc tcgactacct tcgccgaggc ggcctggcgg agatgctcga tgcgctgatc
     2461 gccgacggat ccatcgcgcc cgtggcggcg ctgttcgtcg acaacgcggg ccccggccgg
     2521 cccagcgagt acatggccaa cgacttcacg ctcgcgtccc tcgccgacat ctgtgtgccg
     2581 gcggcggtcg aggagctcgg cctcgagaca cagcatgcgg ccgtcggtcc cggccgggcg
     2641 gcgatcctcg gctcatcgct gggcgggctg atggcgctcc atgcgggcgt gcgccggccg
     2701 gacgtgttcg ggcgggtgat cgcccagtcg accagcgcga tgctcgacga gctgccgctg
     2761 cgcgactcgg ttatccccgc g
//
LOCUS       DATBSW010000226        13536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_374101, whole genome shotgun sequence.
ACCESSION   DATBSW010000226 DATBSW010000000
VERSION     DATBSW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374101"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VIF63_06345"
     CDS             complement(<1..1380)
                     /locus_tag="VIF63_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239035.1"
                     /translation="MLACVVVLGIAGSATVRAATRTWTGLGATNNWSDTGNWSGNAVP
                     GAADIATFDATSSKNATMNVAVNVLGLSVAATYGGTITQSAGIAVTVGATGFSQAGGS
                     FSGGTAGFTVNGPYALSGGTFAATSGTQTVTGAFTISGGAFNAGAGLVSFSGGAATLT
                     VSTADTFNNLTHAAGTKTIAAGTTLTVTGTTTLTAGSLNGAAGTLAAQGDVNQALGYG
                     GGTATLLFSGSGAQTFTGASTTASGSLPLVVIDKPSGTLTLAGTIRTTKDWTYTAGSV
                     DAAASTLVFAGGTITGSQTLNAFDLRATTSIAAGTTLTVTGSTAFTTGALNGTGTLAA
                     QGAISEASTATVGTATLAISGSGAQTLTGTATAAAGSLPLVVINKPSGTLTLAGTIRT
                     STNWTSTAGTIDPGTSTVIFAGGTITGSHTLNIVDVRATTSIAAGSTLTVAGSTMLTS
                     GSLNGTGTLA"
     gene            1588..2994
                     /gene="tig"
                     /locus_tag="VIF63_06350"
     CDS             1588..2994
                     /gene="tig"
                     /locus_tag="VIF63_06350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8239036.1"
                     /translation="MNVTVTPAPRSALNVRVEVPAEKLDSAIAEAVRHLSQRTRIHGF
                     RPGKAPRAVVERAVGAEAVLEEAMDHLVQRAYREALVEKDILPLTQAEVKVEQGEEGK
                     PVIFTATVQVRPEVTLGDYRDFKFKPEIEPIDKAKVDKVVEELRDQNATLAPVEDRGA
                     RNGDYAVIGYTGTRDGVAFDGGSAERMPLIIGEERLIPGFEAHVVGLKPGEKVGFDIA
                     FPDDYPEATLAGQPARFDVELKELREKIMPPEDDDFARQLGTFPDLATLRTEIKARLE
                     RNALDKARHEFADKIIEYAAANATLELPDVLVEQEVEIMHDELRGSLARQGIDEASYL
                     KVTGKSEADLHTDFQPRAEKRVKVLLVLSKVAEAEGLEIGDREVEAEVGKARERYGSD
                     KRTLAYFDSERGRSFIRSTLRRTKLVEKLVDEWLAAHPEHPPLPHLEDDEPVSLADDS
                     VADAPAGTPELEAATASR"
     gene            3006..3707
                     /locus_tag="VIF63_06355"
     CDS             3006..3707
                     /locus_tag="VIF63_06355"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8239037.1"
                     /translation="MLVPMVIESSSRGERAYDIYSRLLKERIIFLGDVIEDHLANLII
                     AQLLFLESEDPEKDISLYINSPGGVVTAGLAIYDTMQYLRAPVSTICIGQAASMASVL
                     LAAGEKGKRYGLPNSRIMIHQGSGGFRGAPVDAIIQMKEWETLVALNHQILARHTGQP
                     IEKIISDTDRDRFMSPEEAKDYGIIDAVYNLQGSSLIAAAHDAGLSGGEGASVTDPSE
                     ATEPEQIPEQKKSKS"
     gene            3753..5063
                     /gene="clpX"
                     /locus_tag="VIF63_06360"
     CDS             3753..5063
                     /gene="clpX"
                     /locus_tag="VIF63_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY8239038.1"
                     /translation="MSTTSGSKGAKVPYRCSFCGKSQEQVRKLIAGQGVYICDECINL
                     CQEIIEEEMLEQPKSKPAAAKLPNPRQIKFALDQYVISQEKAKKVLSVAVYNHYKRVN
                     AGHAVDEVELQKSNVLLVGPTGSGKTLLAQTLARVLDVPFCIADATSLTEAGYVGEDV
                     ENILLRLIQAADFDVARAQRGIIYIDEIDKIARKADNPSITRDVSGEGVQQALLKILE
                     GTVANVPPQGGRKHPHQDFIQIDTTNVLFICGGAFDGLEKIVEERVGKRTIGFGSEAV
                     ASPIDRGKILEKLMPEDLLKFGLIPEFVGRLPVIVTLTALTAADLVRVLTEPKNAIAR
                     QFQKFLSLDKVELVFTPGALQAAAEVALDRKTGARALRSIVEEALLEVMYDIPERSDV
                     RKCIITEETIRSGRVPLLLTKTELDKGVDETNYQDWLAEQGATA"
     gene            complement(5080..6654)
                     /locus_tag="VIF63_06365"
     CDS             complement(5080..6654)
                     /locus_tag="VIF63_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8239039.1"
                     /translation="MRPRTCVVGLDEIGGFRRLQPPLTPFIGLALIALGAVFGALFFS
                     LVIGRHRARQLARLTDRLARATDGKPLSGRIRYPDRVLSSSIERLAGRIADVESLATT
                     DQLTRLINRPACLDVLAGEIGRANRYRRALAVALIDIDHFKRVNDTHGHAVGDEVLRQ
                     VAELLRTTIRGVDTLGRYGGEEFLLVMPETDVEGAMASAEHIRRAIGRTALVVSMPDG
                     QLEVKVTISVGVTGGDGTSSRDIDQMLREADDALYGAKGLGRDQAQAFRVLDEGSKVS
                     SATIAAEARIHASAVGMGAFQASNQHLLDALAERPGWAGGASQLIANLGADIGRAVGL
                     PDGDIDRIRTAALLHDVGKLAIPDEILSKPSALTPIEWRSIVEHPRIGQVVLEQAGAI
                     RDAATIVLHHHEWFDGTGYPHGLAGEEIPIGSRIISIADAYEAMISVRPYKVAMSHQE
                     AVVELRRQRGVQFDPELVEVFLSLVGGPEADPNPAGAGKRAPGSRSVSARGAGSRGGA
                     KSAAGRKVDPGVAHRP"
     gene            6984..9701
                     /locus_tag="VIF63_06370"
     CDS             6984..9701
                     /locus_tag="VIF63_06370"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY8239040.1"
                     /translation="MAELAKAYTPADVEGEIYARWLAADVFAPDGAGSRADTSLPPFV
                     VIQPPPNITGALHLGHALTFAIEDTMVRHARMRGHPTLWLPGLDHASIAAQYVLDKII
                     AADGETRESLGRERYLERMWRFINATREIMLGQERRLGASADWSRLRFTMDEGSARAV
                     RVSFKRLHDDGLAYRTEALINWCPGCMTSVSDLEVLPTPEAGTLWTIRYHLVDDATGD
                     LDPQASIAVATTRPETILGDTAVAVHPDDERYQALVGRRVRIPFVERDVPIIADEVVD
                     RGFGTGAVKVTPAHDKTDHEIGNRHGLPMITVLADDATINEAGDGFKGLDRYEARARI
                     VAELEARGDLVAAKPHEMLIGRCQRSNDVIEPRLKTQWFIRTGPLAEAALRATRDGET
                     SIVPARFEKVWENWLTNIHDWNVSRQLWWGHRIPAWYCPDGHVTVSAEVTGPDACETC
                     RRPARELTQDPDIFDTWYSSGLWPFSTLGWPDATEDFRRYYPGSTMETGYDIIFFWVA
                     RMMMLGIHLADAPPFNKVYLHGLVKDPYGKRMSKTKGNVVDPLEAIDSAGADALRFAL
                     LNGTSPGNDQKFSSERLEDGRNFANKLWNATRFVLGARPASIDADRASRREAPDPERL
                     GPAEHWIRAGARDAVASVDRGFEDDNYGEVSRLLYEAIWSRFCDWGIELAKVRLGDAS
                     LPDADRAATWWTLVEALDTYLRLLHPIMPFVTEALWAVLPHAADDPQLLIVARWPSAG
                     ATSKSNATTEAPVVDEVIELVSAIRTARATAGVPAAAWLPIDIFVPGSHAAVVDALAA
                     AIERLARVRPIARAASREAVDGAPAGSLGVVAGKLEARIHASTQPDAADRARVEKELA
                     QAEAALAATRGRLGDATFVERAPASIVDATRSREAELAERVDRLRASLG"
     gene            9729..11393
                     /locus_tag="VIF63_06375"
     CDS             9729..11393
                     /locus_tag="VIF63_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEY8239041.1"
                     /translation="MATDDWWQTGVVYQIYPRSFADSNGDGIGDLPGILAHLDHLNDG
                     TPKSLGVDAIWLSPIYPSPDFDFGYDVADYVGIDPKYGTLADFEQLVEACHARGMRVI
                     LDLVLNHSSNRHPWFEASRASRTGPFADWYIWGDAVGRSITGARRPPNNWRSFFGGSA
                     WTWEPAREQFYLHTFLPQQPDLNWRNPGTRAALIDVVRTWLDRGVDGFRLDVFNTFFK
                     DAALRSNPRRIGRRGWWGWQRHIHDRDQPELDGALAELRALIDEKPGRMSVGELFDDS
                     FTEAAGYSIPRHLIFDWSLIGLPWNAAAFRAAVERRDGVFGPGRWPANVLSNHDQPRH
                     ASRYDGGDLGDARAKITAAFLLTIRGTPFLYYGEELALRSLDIPNKDAFDPPARRASF
                     LSPWWNRDQARGPMPWRPGPGGGFTSGTPWLPLPPDLDRRNVEREGADPDSVLSFYRR
                     LVWLRRATPALHEGSQELLDLGDADVLAYRRTVPRGSSLVLLNFATRPATVQLPDAAG
                     TNAAAFAASGRRGAWRTALSTHGRASGETLTGPVILAPLEALIAYD"
     gene            11429..12184
                     /locus_tag="VIF63_06380"
     CDS             11429..12184
                     /locus_tag="VIF63_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8239042.1"
                     /translation="MPLEFLRRKGGGTRASKDAVPEEPESTAMPEESVAEEHQLRLNF
                     GAKTSVGVRLQGGPHILAALPQMLGTTAKTPVEVVEPRVTTLASASPNIVHTAEAIEW
                     LRAHGENAPITRHALYVLESMDAIDPAYDTFAVALLDGSVDTSGYPEYASVVGGVAAH
                     WDESTGDLIVRAVVGWGGRGARGDTDRIATKLLQRILSNILESGESLGLTAPDRPGAA
                     PGGASVTCQHCGYPLVSPRAVYCPKCGMRMLHG"
     gene            complement(12212..12598)
                     /locus_tag="VIF63_06385"
     CDS             complement(12212..12598)
                     /locus_tag="VIF63_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239043.1"
                     /translation="MAPVPESLADFFVDPEAPSPDPPSFDSPPLEAAPEAVSFESPPP
                     DEPSPDEPSPDEPSPDEVDPDLERELLLVDDRSFFAQPEPLKWTAGVVIALRNVPSAP
                     QFGQNLGPESLMPWITSVRCRQVAQT"
     gene            12629..12871
                     /locus_tag="VIF63_06390"
     CDS             12629..12871
                     /locus_tag="VIF63_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239044.1"
                     /translation="MPPAATDWISLEEAAGILSAANVRFQPATIGGWARAGKVQSIKL
                     GGRRFVRRGEIKALIAAPRRVRAADLQPGLFEDLRD"
     gene            13000..>13536
                     /locus_tag="VIF63_06395"
     CDS             13000..>13536
                     /locus_tag="VIF63_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8239045.1"
                     /translation="MLAGGLAYAALFAIVPAVILLAGVVGLVYADPVDRAQMVAVLVG
                     VLPPMRDLIETVVTEAARDATQVSIVGAALLVWGASRFVVAFDGAIARIMGGDKARGV
                     LAQNLAALGAVVLLVLAIPASAVLSTVAAFLDEGQSIGVLQAVGAALSIALGVLPAFA
                     TIGAMVLVYRVVPTPAPRW"
BASE COUNT         2096 a         4728 c         4746 g         1966 t
ORIGIN      
        1 ggcgagggtg ccggtgccgt tgaggctgcc ggacgtgagc atggtcgatc cggcgacggt
       61 cagggtggag ccggcggcga tcgaggtcgt ggctcggacg tcgacgatgt tgagggtgtg
      121 gctgccggtg atcgtcccgc cggcgaagat gaccgtcgac gtccccgggt cgatcgtccc
      181 ggcggtggac gtccagttgg tgctcgtccg gatcgtgccc gccagggtga gcgtgcccga
      241 cggcttgttg atgaccacca ggggcaggct gccggcggcg gccgtggccg tcccggtcag
      301 ggtctgggca ccggagccgc tgatcgccag cgtggccgtc ccgacggtcg cggtggaggc
      361 ctcgctgatg gcgccctggg cggcgagggt gccggtgcca ttgagcgcgc cggtggtgaa
      421 cgccgtcgag ccggtcacgg tcagggtcgt gcccgcggcg atcgaggtcg tcgcccgcag
      481 gtcgaacgcg ttgagggtct ggctgccggt gatcgtgccg ccggcgaaga cgagggtcga
      541 ggccgccgca tcgacgctgc ccgcggtata cgtccaatcc ttggtcgtcc ggatcgtgcc
      601 cgccagggtg agcgtgcccg acggcttgtc gatgaccacc aggggcaggc tgccgctggc
      661 cgtggtcgag gcgccggtga atgtctgcgc gccggatccc gagaagagca gggtggccgt
      721 gccgccgccg tagccgaggg cctggttgac gtcgccctgg gcggcgaggg tccccgcggc
      781 gccgttgagg ctgcccgcgg tgagcgtcgt cgtgccggtc acggtcagcg tcgtgccggc
      841 ggcgatcgtc ttcgttcccg cggcatgggt caggttgttg aacgtgtcgg ccgtcgacac
      901 ggtgagcgtg gcggctccgc cgctgaacga cacgaggccg gcacccgcgt tgaacgcgcc
      961 gccactgatc gtgaacgcgc cggtcaccgt ctgcgttccg gatgtcgcgg cgaacgtccc
     1021 gccgctcagc gcatacggac cgttcaccgt gaagccggcc gtaccgccgc tgaacgagcc
     1081 gccggcctgg ctgaagccgg tggccccgac ggtgacggca atgcccgcgc tctgcgtgat
     1141 cgtcccgcca tacgtcgctg cgaccgacag cccgaggaca ttcacggcga cgttcatcgt
     1201 ggcgttcttg ctgctcgtcg cgtcgaaggt cgcgatatcg gctgcaccgg gcaccgcatt
     1261 gcccgaccag ttgccggtgt cggaccagtt gttcgtggcg ccgagcccgg tccaggtccg
     1321 tgtcgcggcg cgaaccgtcg ccgacccggc gatgcccagt acgactacgc aggccagcac
     1381 ggcggcaagc gcgaaagagg cccgccaaag ggcggaccta ccacttggcg ctcgcacgcc
     1441 gatcacgcgc cacctccgct tagcacttga gcgcagcgga ccacaccaag ccagtgcgtt
     1501 ccacccgccc gaagcgcctc cccccgcaaa ctttggtact gggggtccca acggccgcga
     1561 cgtccgccgc acctatactc ccgctcgatg aacgtcacgg tcacgcccgc accccgcagc
     1621 gcgctcaacg tgcgcgtcga ggtgcccgcc gagaagctcg acagcgcgat cgccgaggcg
     1681 gtccggcacc tgtcgcagcg cacccggatc cacggcttcc ggccgggcaa ggcgccgcgc
     1741 gcagtggtcg agcgcgcggt cggcgcggag gccgtcctcg aggaggcgat ggaccatctc
     1801 gtccagcgcg cctatcgcga ggcgctggtc gagaaagaca tcctgccgct gacccaggcc
     1861 gaggtgaagg tcgagcaggg cgaagagggc aagccggtca tcttcacggc gaccgtgcag
     1921 gtccggcccg aggtgaccct cggcgactat cgtgacttca agttcaagcc cgagatcgag
     1981 ccgatcgaca aggccaaggt cgacaaggtc gtcgaggagc tgcgcgacca gaacgcgacc
     2041 ctcgccccgg tcgaggaccg cggcgcgcgc aacggcgact acgcggtgat cggctacacg
     2101 gggacccgtg acggcgtcgc gttcgatggc gggagcgcgg agcgcatgcc cctgatcatc
     2161 ggcgaggagc ggctgatccc ggggttcgag gcgcacgtgg tcggcctcaa gcccggcgag
     2221 aaggtcgggt tcgacatcgc cttccccgac gactatcccg aggcgacgct cgccggccag
     2281 cctgcccgct tcgacgtcga gctcaaggag ctgcgcgaga agatcatgcc gcccgaggac
     2341 gacgacttcg cgcgccagct cgggacgttc cccgacctcg ccaccctccg aaccgagatc
     2401 aaggcccgcc tggagcgcaa cgccctcgac aaggctcgcc acgagttcgc ggacaagatc
     2461 atcgagtacg ccgcagcgaa cgcgacgctc gagctgcccg acgtcctggt cgagcaggag
     2521 gtcgagatca tgcacgacga gctgcgtggc tccctcgccc ggcaggggat cgacgaggcg
     2581 tcgtacctca aggtcaccgg caagagcgag gccgacctcc acacggactt ccagccccgc
     2641 gccgagaagc gcgtgaaggt cctgctcgtc ctctcgaagg tggccgaggc ggaaggcctc
     2701 gagatcggcg accgcgaggt cgaggccgag gtgggcaagg cccgcgagcg ctacggcagc
     2761 gacaagcgga cgctggccta cttcgactcc gagcggggcc gcagcttcat tcgcagcacc
     2821 ctccgccgca ccaagctcgt cgagaagctc gtggacgagt ggctcgccgc gcaccctgag
     2881 cacccgccac tcccgcatct cgaggatgac gagccggtca gcctcgccga cgactcggtc
     2941 gccgacgcgc ccgccggcac gcccgagctc gaagccgcca ccgccagtcg ctgaggagat
     3001 tccgaatgct cgtcccgatg gtcatcgagt cctccagccg gggggagcgc gcctacgaca
     3061 tctactcgcg cctgctgaag gagcggatca tcttcctcgg cgacgtgatc gaggatcacc
     3121 tcgcgaacct gatcatcgcc cagctgctgt tcctcgagtc cgaggacccg gagaaggaca
     3181 tcagcctgta catcaactcg cctggtggcg tcgtgacggc cgggctggcg atctacgaca
     3241 cgatgcagta cctgcgcgcg ccggtgagca ccatctgcat cggccaggcg gcgtcgatgg
     3301 catccgtgct gctcgcggcc ggcgagaagg gcaagcgcta cggcctgccg aacagccgga
     3361 tcatgatcca ccagggctcg ggcgggttcc gcggcgctcc cgtggacgcg atcatccaga
     3421 tgaaggagtg ggaaacgctc gttgcgttga accaccagat tcttgcccgc cacacgggcc
     3481 agccgatcga aaagatcatc agcgacacgg atcgcgaccg gttcatgtct cccgaagagg
     3541 caaaggacta tggcatcatc gatgcggtct acaacctcca ggggtcgtca ctgatcgctg
     3601 cagcccacga tgccggactc tccggcggcg agggtgccag cgtcacggac ccgtcggaag
     3661 cgacggagcc ggagcagatc ccggagcaga aaaagtcgaa gtcctgatcc cggtccggcc
     3721 gggcgggccg cgacggcagg caaggggatc ggttgagcac gacaagcggg agcaagggcg
     3781 cgaaggtccc gtatcgctgc tcgttctgcg gcaagagcca ggagcaggtc cgcaagctca
     3841 tcgcgggcca gggcgtctac atctgcgacg agtgcatcaa cctctgccag gagatcatcg
     3901 aagaggagat gctcgagcag ccgaagtcga agccggctgc ggcgaagctc ccgaacccgc
     3961 gccagatcaa gttcgcgctc gaccagtacg tgatcagcca ggagaaggcc aagaaggtcc
     4021 tctcggtggc cgtctacaac cactacaagc gagtcaatgc cggccacgcg gtcgacgagg
     4081 tcgagctcca gaagtcgaac gtcctgctcg tcgggccgac gggctcgggc aagaccctgc
     4141 tcgcgcagac gctcgcgcgc gtgctcgacg tgccgttctg catcgccgac gcgacgtcgc
     4201 tcacggaggc gggctacgtc ggcgaggacg tcgagaacat cctgctgcga ctgatccagg
     4261 ccgccgactt cgacgtcgcc cgggcccagc gcgggatcat ctacatcgac gagatcgaca
     4321 agatcgcgcg caaggcggac aacccctcga tcacccgtga cgtttcgggc gagggcgtcc
     4381 agcaggcgct gctcaagatc ctcgagggga ccgtcgccaa cgtcccgccc cagggcggcc
     4441 gcaagcatcc ccaccaggac ttcatccaga tcgacaccac gaacgtgctg ttcatctgcg
     4501 gcggcgcgtt cgacggcctg gagaagatcg tcgaggagcg cgtcggcaag cgcacgatcg
     4561 gcttcggctc ggaggcggtc gcgagcccga tcgaccgcgg caagatcctc gagaagctga
     4621 tgcccgagga cctgctcaag ttcgggctca tccccgagtt cgtcggccgc ctgccggtca
     4681 tcgtgaccct caccgcgctc acggccgcgg acctcgtccg cgtcctgacc gagccgaaga
     4741 acgcgatcgc ccggcagttc cagaagttcc tctcgctcga caaggtcgag ctcgtgttca
     4801 cgcccggcgc gctccaggct gcggccgagg tcgcgctcga tcgcaagacg ggtgctcgcg
     4861 ccctccgctc gatcgtggaa gaagcgctgc tcgaggtgat gtacgacatc cccgagcgct
     4921 ccgacgtgcg caaatgcatc atcaccgagg agacgatccg gtcgggccgg gtgccgctgc
     4981 tcctcacgaa gaccgagctg gacaagggcg tcgacgagac gaactaccag gactggctgg
     5041 ccgagcaggg cgccacggcc tgaggttccc cggcgctcgt cagggccggt gcgcgactcc
     5101 cggatcgacc tttcgtcccg ccgcagactt ggcgccgccg cgtgagccgg cgcctcgcgc
     5161 actgacgctc cgcgagccgg gcgcgcgttt gccggcgccg gccgggttcg ggtcggcctc
     5221 gggtccgccc acgagcgaca ggaagacctc gacgagctct ggatcgaact gcacaccacg
     5281 ctggcggcgg agctcgacga ccgcctcctg gtgggacatc gcgaccttgt acggccgaac
     5341 ggagatcatc gcctcgtagg cgtcggcgat cgagatgatc cgcgagccga tcgggatctc
     5401 ctcacccgcg agcccgtggg ggtagcccgt gccgtcgaac cactcgtggt gatgcagcac
     5461 gatggtggcg gcgtcgcgaa tcgcgccggc ctgctcgagc acgacctgac cgatccgtgg
     5521 gtgctcgacg atcgaacgcc actcgatcgg cgtcagcgcc gacggcttcg acaggatctc
     5581 gtcggggatg gcgagcttgc ccacgtcgtg cagcagcgcc gcggtgcgga tccggtcgat
     5641 gtcgccgtcc ggcaggccga ccgcccgacc gatgtcggcg ccgagattcg cgatcagctg
     5701 tgacgcgccg ccggcccagc cagggcgctc ggcgagggca tcgagcaggt gctggttgga
     5761 tgcctggaat gcgcccatgc cgacggccga cgcatggatc cgtgcctcgg cagcgatggt
     5821 cgcgctggac accttggagc cttcatcgag gacgcggaac gcctgggcct ggtcgcgccc
     5881 gaggcccttc gccccgtaga gcgcgtcgtc ggcctcgcgc agcatctggt cgatgtcccg
     5941 gctcgaggtg ccatcaccac cggtgacgcc gacgctgatc gtgaccttga cctcgagctg
     6001 gccatcgggc atcgagacga cgagcgccgt ccggccgatg gcgcgccgga tgtgctcggc
     6061 cgacgccatg gcaccctcga cgtcggtctc gggcatgacc agcaggaact cctcgccacc
     6121 gtatcgaccg agcgtgtcga cgccgcggat cgtggtgcgg aggagctcgg cgacctggcg
     6181 gagcacctcg tcaccgacgg cgtggccgtg cgtgtcgttg acgcgcttga agtggtcgat
     6241 gtcgatcagg gccacggcga gggcgcggcg atagcggttc gctcgcccga tctcgccggc
     6301 gaggacgtcg aggcacgccg gccggttgat gaggcgggtc agctggtccg tggtggccag
     6361 cgactccacg tcggcgatgc ggccggcgag ccgttcgatg gacgagctca gcacacggtc
     6421 cgggtagcgg atccgacccg acagcggctt gccgtcggtg gctcgggcga gccggtcggt
     6481 cagtcgggcg agctgtcggg cgcggtggcg gccgatgacg agggagaaga acagggcccc
     6541 gaagaccgcg ccaagggcga tgagcgcgag gccgatgaac ggtgtcagag gtggctgcaa
     6601 gcggcggaat cccccgatct cgtccaggcc cacgacgcag gtgcgtggtc gcaccaaccg
     6661 tacgatttcg gcgctccacg gggcatagcc caaaggtccc cccaactgtc ccgatgtaca
     6721 ctttgcccct cacggcgagg accgagagga gtagcgtccc aacgggccca cgagcgagtg
     6781 cgggatggtg tgagccgcgc gggttcccgg gtgcgaatgt cgctcgcgag ccgtgcggtt
     6841 ggcgcccgat agcgcgccga gagggcctcg ggaccagtcc ggcgcgatga gcgacgcgga
     6901 gcccgatgcc gaaccagcag gtggtaccac gaccccgttc gtccgctgga cgagcggggc
     6961 atttgattct tgacggagga cacgtggcag agctcgcgaa ggcgtacacg ccggccgacg
     7021 tggaagggga gatctacgcg cgctggctgg cggcggacgt cttcgccccc gacggcgccg
     7081 gatcgcgcgc ggacacgtcc ttgccgccgt tcgtcgtgat ccagccgccg ccgaacatca
     7141 ccggcgcgct gcacctcggc cacgccctca cgttcgccat cgaggacacc atggtccggc
     7201 atgcccgcat gcgcggccac ccgacgctct ggctgcccgg gctcgaccac gcctcgatcg
     7261 cggcgcagta cgtcctggac aagatcattg ccgccgacgg cgagacgcgg gagtcgctcg
     7321 gacgcgagcg ctacctcgag cggatgtggc ggttcatcaa cgcgacccgc gagatcatgc
     7381 tcggccagga gcggcggctc ggtgcctcgg cggactggtc gcgcctgcgg ttcacgatgg
     7441 acgaggggtc ggcgcgcgcg gttcgggtct cattcaagcg gctccatgac gacggcctgg
     7501 cgtaccgcac cgaggcgctg atcaactggt gccccggatg catgacgagc gtgtcggacc
     7561 tcgaggtcct gcccacgcca gaggcgggca cgctgtggac gatccgctac cacctcgtcg
     7621 acgacgcgac gggcgacctc gacccacagg catcgatcgc cgtcgcgacg acgcggccag
     7681 agacgatcct cggcgacacc gcggtggcgg tccatcccga cgatgagcgc taccaggcgc
     7741 tggtcgggcg gcgcgtgcgc atcccgttcg tcgagcgcga cgtgccgatc atcgcggacg
     7801 aggtggtcga tcgtgggttc ggcaccggtg cggtcaaggt cacgcctgcc cacgacaaga
     7861 ccgaccatga gatcgggaat cgccacggcc tgcccatgat cacggtcctc gccgacgacg
     7921 cgacgatcaa cgaggcaggc gacggcttca agggtctgga tcggtacgag gcgcgagcac
     7981 gcatcgtggc ggagctcgag gcgcgcggcg atctcgtcgc agccaagccg cacgagatgc
     8041 tgatcgggcg ctgccagcgt tccaacgatg tcatcgagcc gcgactcaag acccagtggt
     8101 tcattcgcac cgggccgctg gccgaggcgg ccctgcgcgc cacgcgcgac ggcgagacca
     8161 gcatcgtgcc ggcccgcttc gagaaggtct gggagaactg gctcaccaac atccacgact
     8221 ggaacgtgag ccgccaactg tggtggggtc accgcatccc ggcgtggtac tgcccggacg
     8281 gccacgtgac cgtgtccgcc gaggtcaccg gacccgacgc ctgcgagacg tgccgccgcc
     8341 cggcgcgcga gctgacccag gacccggaca tcttcgacac ctggtacagc tccgggctgt
     8401 ggccgttctc gacgctcggc tggcccgacg ccaccgagga cttccgccgc tactacccgg
     8461 gatccacgat ggagacgggc tacgacatca tcttcttctg ggtcgcccgg atgatgatgc
     8521 tgggcatcca cctcgccgac gccccgccgt tcaacaaggt ctacctgcac gggctggtga
     8581 aggacccgta cggcaagcgg atgtcgaaga cgaagggcaa cgtcgtcgat ccgctcgagg
     8641 cgatcgactc ggccggcgcg gacgcgctcc gattcgccct gctgaacggg acctcgccgg
     8701 gcaacgacca aaagttcagc agcgagcggc tcgaggacgg ccggaacttc gccaacaagc
     8761 tttggaacgc cacgcgcttc gtcctcggcg cccggccggc gtcgatcgat gcggatcgcg
     8821 cgtcccggcg cgaggcgccg gacccggagc ggctcgggcc ggccgagcac tggatccggg
     8881 ccggggcacg ggacgccgtc gcgtcggtcg atcgcggatt cgaggacgac aattacggcg
     8941 aggtctcgcg cctcctgtac gaggcgatct ggagccggtt ctgcgactgg ggcatcgagc
     9001 tggcgaaggt gcggctcggc gatgcgtccc tgcccgacgc ggatcgcgcg gcgacgtggt
     9061 ggacgctggt cgaagcgctc gatacgtacc tgcggctact ccacccgatc atgccgttcg
     9121 tcaccgaagc gctgtgggcc gtgctgccgc acgccgctga cgacccgcag ctcctgatcg
     9181 tcgcgcgctg gccgtcggcc ggggccacgt cgaagtcgaa cgcgaccacg gaggcgccgg
     9241 tcgtcgacga ggtcatcgag ctcgtgtccg ccatccgaac cgctcgcgcc accgccggtg
     9301 tcccggccgc tgcgtggctg ccgatcgaca tcttcgtgcc gggctcgcac gccgcggtcg
     9361 tcgacgcgct cgcggcggcg atcgagcggc tggcgcgcgt ccgcccgatc gctcgcgccg
     9421 caagccgcga ggccgtcgac ggcgcgccgg ccgggtccct cggggttgtc gcgggcaagc
     9481 tggaggcgcg catccacgcc tcgacacagc cggacgcggc cgaccgcgcc cgggtcgaga
     9541 aggagctggc ccaggccgag gccgccctcg ccgcgacgcg cggccggctc ggcgatgcca
     9601 cgttcgtcga acgcgccccg gcctcgatcg tcgatgccac ccgcagtcgc gaggccgagc
     9661 tggccgagcg cgtcgaccgc ctgcgggcct cgctgggcta gcgcgccgtg ggtggcacac
     9721 tgcgatcgat ggcgaccgac gactggtggc agaccggcgt cgtctaccag atctatccgc
     9781 gcagcttcgc cgacagcaac ggcgacggga tcggcgacct gcccggcatc ctcgcccacc
     9841 tcgatcacct caacgacggc acgccgaagt cgctcggcgt cgatgcgatc tggctctcgc
     9901 cgatctaccc gtcacccgac ttcgacttcg gctacgacgt ggcggactac gtcggcatcg
     9961 acccgaaata cggcaccctc gctgatttcg agcagctcgt cgaggcgtgc cacgcccgcg
    10021 ggatgcgggt catcctcgac ctcgtcctca accacagcag caaccggcac ccctggttcg
    10081 aggcgtcgcg ggcgagccgg accgggccgt tcgccgactg gtacatctgg ggcgacgctg
    10141 ttggtcgttc gatcaccggc gcgcggcgcc cgccgaacaa ctggcggtcg ttcttcggcg
    10201 gctcggcgtg gacctgggag ccggcgcgcg agcagttcta cctgcacacg ttcctgcccc
    10261 agcagcccga cctgaactgg cgcaacccgg ggacgcgcgc ggcgctcatc gacgtcgtcc
    10321 ggacgtggct cgatcgcggc gtcgacggct tccggctcga cgtgttcaac accttcttca
    10381 aggacgcggc gctgcgctcg aacccgcgca ggatcgggcg gcgcggctgg tggggctggc
    10441 agcgccacat ccacgaccgc gaccagccgg agctcgacgg ggcgctcgcc gagctgcggg
    10501 cgctcatcga cgagaagccc gggcgcatgt ccgtcggtga gctgttcgat gactcgttca
    10561 ccgaagccgc tggctattcg atcccgcgcc acctgatctt cgactggtcg ttgattggcc
    10621 tgccgtggaa cgccgccgca ttccgagccg cggtcgagcg gcgagacggc gtcttcgggc
    10681 cgggtcggtg gcctgcaaat gtcctctcga accacgacca gccgcgccac gcgagccggt
    10741 acgacggtgg cgacctcggc gacgcgcggg ccaagatcac ggctgcattc ctgctcacca
    10801 tccggggcac gccgttcctg tactacggcg aagagctcgc cctccgcagc ctcgacatcc
    10861 ccaacaagga cgcgttcgac ccgcccgccc gccgggcgtc gttcctgtcg ccgtggtgga
    10921 accgagatca ggcgcgcggc ccgatgccgt ggcgacccgg cccgggcggc ggattcacga
    10981 gcggcacacc gtggttgccg ctgccgccgg atctcgaccg gcgcaacgtc gagcgcgagg
    11041 gggcagaccc cgactcggtg ctctcgttct atcgccgcct ggtgtggctc cgacgtgcaa
    11101 cgcccgccct tcacgaggga tcgcaggagc tgctggacct cggcgacgcc gatgtccttg
    11161 cctatcgccg cacggtcccg cggggatcct cgctcgtcct gctcaacttc gcgacgcggc
    11221 cggcgacggt gcagctcccg gatgctgcag ggaccaatgc cgcagcgttc gcggcctccg
    11281 ggcgccgggg agcctggcga accgccctct cgacccacgg gcgggcatcg ggcgagacgc
    11341 tcaccggacc ggtgatcctc gcgccgctgg aggcgctcat cgcctacgat tgagccctcg
    11401 cacacagacc gatcggggag attgcttcgt gccgctcgaa ttcctgcgtc gcaagggtgg
    11461 gggaacccgt gcgtcgaagg acgccgtccc cgaggaaccc gaatcgacag ccatgccgga
    11521 ggagtccgtc gccgaggagc accagctccg gctgaacttc ggcgccaaga cgagcgtcgg
    11581 cgtgcggctg cagggcggcc cgcacatcct cgccgcgctg ccccagatgc tgggcacgac
    11641 cgccaagacc ccggtcgagg ttgtcgagcc tcgggtgacg accctcgcct ccgcgtcgcc
    11701 caacatcgtc cacacggccg aggccatcga atggctgcgc gcccatggcg agaacgcgcc
    11761 gatcacgcgc cacgccctct acgtcctcga gtcgatggac gccatcgacc cggcgtacga
    11821 cacgttcgcg gtcgcgctgc tggacggcag cgtcgacacg agcgggtacc cggagtacgc
    11881 gtcggtcgtc ggcggggtcg ctgcccactg ggacgaatcg actggcgacc tgatcgtccg
    11941 tgccgtcgtc ggctggggcg ggcgcggcgc gcggggcgac accgaccgga tcgccacgaa
    12001 actgctccag cggatcctca gcaacatcct cgaaagcggc gagtcgctgg ggctgacggc
    12061 acccgatcga ccgggcgctg cgcccggcgg ggcgagcgtg acgtgccagc actgtggcta
    12121 tccattggtg agtccccgtg ccgtgtactg ccccaagtgc ggcatgcgga tgctgcacgg
    12181 ctgatccgga cgctcggttg ccgatctacg actacgtctg cgcgacctgc cggcaccgga
    12241 ccgaggtgat ccacggcatc aacgactccg gcccgaggtt ctgcccgaac tgcggcgccg
    12301 aaggaacgtt gcgcaaggcg atgacgacgc ccgcggtcca tttcaagggc tccggctggg
    12361 cgaagaagga tcgatcgtcg acgagcagca gctcgcgctc gaggtcgggg tcgacctcgt
    12421 ccggcgacgg ctcgtccggc gacggctcgt ccggcgacgg ctcgtcgggc ggtggcgatt
    12481 cgaaggacac ggcctctggc gccgcctcga gcggcggcga gtcgaaggac ggcgggtccg
    12541 gcgagggcgc ttccgggtcc acgaagaaat ctgcaagtga ttccggtacg ggggccaccg
    12601 gctcgaaatc gagcagcgag gactgacgat gccacctgcc gccaccgact ggatcagcct
    12661 cgaggaggcc gccggcattc tctctgccgc gaacgttcgc ttccagcccg cgacgatcgg
    12721 cggctgggcg cgcgccggca aggtccagag catcaagctc ggcgggcgcc ggttcgtgcg
    12781 tcggggcgag atcaaggccc tgatcgcggc gccgcgcagg gtgcgcgccg cggacctcca
    12841 gccgggcctg ttcgaggatc ttcgggactg aacgacccgc ggccgccgtc cgcggaccgg
    12901 ctcgctgggc tccgccggcg ggccgggatc gtcctcgacc aacccgccgt cgcggcgggc
    12961 cgtcgaatcc tggatcggtt cggcgcggca ggcggcggtc tgctcgcagg cggcctggcc
    13021 tatgcggcgc tgttcgcgat cgtacctgcg gtgatcctgc tcgctggcgt cgtcggcctg
    13081 gtctacgccg acccggtcga tcgcgcccag atggttgccg tcctcgtcgg cgtgctgccg
    13141 ccgatgcgcg acctgatcga gacggtggtg accgaggcgg cacgagacgc gacacaggtc
    13201 tcgatcgtgg gcgccgcgct gctcgtctgg ggcgccagcc ggttcgtcgt cgccttcgat
    13261 ggcgcgatcg cgcggatcat gggtggggac aaggcgcgcg gtgtgcttgc gcagaacctc
    13321 gctgccctcg gtgcggtcgt gctcctggtg ttggccattc ccgcgagcgc ggtgctctcc
    13381 acggtggccg cgttcctcga cgaaggccag tcgatcggtg tcctccaggc tgtgggcgct
    13441 gccctgtcca tcgcgctcgg tgtcctgccg gccttcgcca cgatcggggc gatggtgctc
    13501 gtctaccgcg tcgtcccgac tcccgccccg aggtgg
//
LOCUS       DATBSW010000227         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_727096, whole genome shotgun sequence.
ACCESSION   DATBSW010000227 DATBSW010000000
VERSION     DATBSW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727096"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1524
                     /locus_tag="VIF63_06400"
     CDS             <1..>1524
                     /locus_tag="VIF63_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8239046.1"
                     /translation="LDDLQYQGFDAVVIAIGCYDTNKLGIPGEDAPEVLDGLEYLRTA
                     TLGLPYPEHAGKRVVVIGGGFTSMDCSRTSIRQGAKEVTLVYRRDMKDMPASNEVHEA
                     IEEGVTAIFQAGPVRVITDKDNNVTGVEFIRMALGAPDASGRRRPEPAPGTEFTIPCD
                     RVLLAIGQGPELDWIGPGSTGPQATKNKRLQADAVTFETARPGVFGTGDVRIGAATVV
                     QAIAEGRRAAYAVDAFLRGNDLSTIRTRQTLAEPEPIFLSIVPFTSEVKEPRYRMKAM
                     EAEERNRNYVEYEIPYSREEIVAESTRCLQCTCEAIGFCDLRRLGIEYETTLTTLEPA
                     PHSGAGLRSVNENRFTGVNHDYVRDDSHAFILREPSRCIDCGRCAQVCSEVVGAACYD
                     FMRIGFDTLVTTPLDMSLNDTPCVSCGRCAETCPTGALMPKPRVLQKYEVDESRCILC
                     GICVDACPYDALRDGGEFELAHSSREEPMIDLMAMAQVDRETEVTYIRKERDWLQRAI
                     "
BASE COUNT          249 a          551 c          505 g          219 t
ORIGIN      
        1 ctcgacgacc tgcagtacca gggcttcgac gccgtcgtca tcgcgatcgg ctgctacgac
       61 acgaacaagc tcggcatccc gggcgaggat gcgccggagg tcctcgacgg cctcgaatac
      121 ctgcgcaccg cgacgctcgg cctgccgtac ccggagcacg cgggcaagcg cgtcgtcgtc
      181 atcggcggcg gcttcacctc gatggactgc tcgcggacat cgatccgcca gggcgccaag
      241 gaagtgacgc tcgtctaccg ccgcgacatg aaggacatgc cggcgtcgaa cgaggtccac
      301 gaggcgatcg aggaaggcgt cacggcgatc ttccaggccg gcccggttcg ggtcatcacc
      361 gacaaggaca acaacgtcac cggcgtcgag ttcatccgga tggccctcgg cgccccggat
      421 gcctccggcc ggcgccgtcc ggaaccggct ccgggcacgg agttcacgat cccctgcgac
      481 cgcgtgctgc tggcgatcgg ccagggcccc gagctcgact ggatcgggcc cggctcgacc
      541 ggaccccagg cgacgaagaa caagcgcctc caggccgacg cggtgacgtt cgagacggcc
      601 cggcctggcg tgttcggcac cggcgacgtc cggatcggtg ccgccacggt cgtccaggcc
      661 atcgccgagg gccgccgcgc cgcctacgcg gtcgatgcgt tcctgcgtgg caacgacctg
      721 tcgaccatcc gcacccgcca gacgcttgcc gagcccgagc cgatcttcct gtcgatcgtg
      781 cccttcacct ccgaggtgaa ggagccgcgc taccgcatga aggccatgga ggccgaggag
      841 cgcaaccgga actacgtcga gtacgagatc ccgtactccc gcgaggagat cgtcgcggaa
      901 tcgacgcgct gcctccagtg cacgtgcgag gccatcgggt tctgcgacct gcgccgcctc
      961 ggcatcgagt acgagacgac gctgacgacg ctcgagccgg cgccgcactc gggcgcgggc
     1021 ctgcgctcgg ttaacgagaa ccggttcacc ggcgtgaacc acgactatgt tcgcgacgac
     1081 tcgcacgcgt tcatccttcg cgagccctcg cgctgcatcg actgcgggcg ctgcgcccag
     1141 gtctgctccg aggtcgtcgg cgccgcgtgc tacgacttca tgcgcatcgg cttcgacacg
     1201 ctcgtgacga cgccgctcga catgagcctc aacgacaccc cgtgcgtgtc ctgcggccgc
     1261 tgcgcggaga cctgcccgac gggcgcgctg atgccgaagc cgcgcgtcct ccagaagtac
     1321 gaggtcgacg agagtcgctg catcctgtgc gggatctgtg tcgatgcctg cccgtacgac
     1381 gccctccgcg acggcggcga gttcgagctc gcccactcga gccgcgagga gccgatgatc
     1441 gacctcatgg cgatggccca ggtcgaccgc gagaccgagg tcacctacat ccgcaaggag
     1501 cgcgactggc tgcagcgggc gatc
//
LOCUS       DATBSW010000228         5785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_304008, whole genome shotgun sequence.
ACCESSION   DATBSW010000228 DATBSW010000000
VERSION     DATBSW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304008"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VIF63_06405"
     CDS             <1..1244
                     /locus_tag="VIF63_06405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY8239047.1"
                     /translation="RIIQNVKTAGTNNPVFMLDEIDKVGMDFRGDPSSALLEVLDPEQ
                     NNSFQDNYLEVPFDLSKVLFVATGNLIDPIPMALRDRMEIIQVPGYTQREKVEIGKRF
                     LIPKQMENHGLKPKNIVFTDEAMTELVQAYTHEAGVRNLEREIANIMRKVARKVAEGR
                     KAKTVIDNKRLLEFLGPPRFEYGELEPEDQVGSATGLVVTEVGGDVVAIEVTRMDGKE
                     DFILTGQLGEVMRESARAGLSWIRTHAAELGISRETFEKQTLHIHVPAGATPKDGPSA
                     GITMALAMVSAYTGIPVRKDLAMTGEITLRGRVLPIGGLKSKILAAHLAGARMVVIPR
                     KNEKDLRDIPDEIRKQIKIVLVDSMNEVLETALRRRPKPLPATPKSAPATDPVKEPET
                     EPAPIRRPNFPPDQPTVVVQG"
     gene            1255..2361
                     /locus_tag="VIF63_06410"
     CDS             1255..2361
                     /locus_tag="VIF63_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8239048.1"
                     /translation="MASPEFQDYYKTLGVPRTATQAEIKKAFRKLARDSHPDKHPGDA
                     AAERRFKSINEANAVLSDTAKRAKYDQFGADWEAYARAGASAGGRGGADPFGPGSPFA
                     GFGGFSAGGARAGAGTGPGGVRFEFRSTGGDAGAFSDFFHMMFGDEGAAAGEPERFAR
                     STSGESIDDLLARMGIAGAGGAAAGARPRAGAAPRGGAAAAGPPIEVTAEISLEESFH
                     GTSRVIELDGRRLEVTIPRGADTGNRIRLTGKGPSGRDLVVAIKLKPHKVFTRRGHDL
                     EREVPVTLREALLGGEIPVGTPKGKVLLKVPAGSQNGRTIRLKGQGMPHFKGDGTGDL
                     FVRVRVVLPTDLSDEDAAAAAKVLDKIQQPDPRA"
     gene            2390..5182
                     /gene="clpB"
                     /locus_tag="VIF63_06415"
     CDS             2390..5182
                     /gene="clpB"
                     /locus_tag="VIF63_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY8239049.1"
                     /translation="MQLDHFTEKAQEAIVAAQQLAGRLDSPVLDAEHLLSALVEPDDG
                     VPAETLRRLGVDLPGFRGEVAAILAKRARIQGGSLALDSRAKRVIDRAQEEARRLGDE
                     YTSTEHLLIGIAEIGGEAQKLLERHNAGKEQLLTALQSVRGGQRVTSPNPEGTYQALE
                     KYGRDLTAEARAGRLDPVIGRDDEVRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVRGDVPETLKDKRVVALDLGALIAGAKFRGEFEERLKAVLKEIKDSAGRVILFID
                     ELHTVVGAGAAEGAMDASNLLKPMLARGELHSIGATTLDEYRKHIEKDAALERRFQQV
                     YVDQPTVEETISILRGLRERYEVHHGVRITDSALVAAATLSDRYITERFLPDKAIDLV
                     DESASRLRMEIDSMPVELDEIERRRIQLEIEREALRKEKDDASKARLQALEKELAEIA
                     EEGGAMKQRWEGEKAAIQAIRATKSELEGLQVQIEQAEREMNYERAAQLKYGTQRELQ
                     TRLAGQEAALAALQGPNSLLKEEVTADEIADIVAKWTGIPVTRLLEGELAKLVHMEER
                     LHERVVGQDEAIAAVSDAVRRARAGLKDPKRPIGSFLFLGPTGVGKTELARALAAFMF
                     DDEHAMTRIDMSEYMEKFAVSRLIGAPPGYVGYDEGGQLTEAIRRRPYQVVLLDEIEK
                     AHPDVFNVLLQVLDDGRLTDSQGRTVDFKNALIIMTSNVGSQAIAAVGGRPGEGEAEA
                     YEAMKRQVTDALRLQFRPEFLNRVDEVIVFHALTEADLAAIVELLVAELQRRLAAQDL
                     VLELTPAARALIAREGTDPTFGARPLKRTIQRLVENPLARALIEGSFKPGQTVHVDAD
                     PVGTTLVFSADGQSVVTDAGNRRDARRRSDDEGLEPVGAGSSGGRGKRRGSVLDLPDT
                     DPERGEGGNGGGGRPN"
     gene            5251..>5785
                     /locus_tag="VIF63_06420"
     CDS             5251..>5785
                     /locus_tag="VIF63_06420"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8239050.1"
                     /translation="MPIRIDGAPWKLPADGMDSARPAAVLVLVAPDEAGEARVVLIER
                     TSYDGHHSGEIAFPGGAAEPGDASLVETALREAAEEIGLEPAAIELRVIGELEPFWIP
                     VSNFRVTPVLAVANRLPAWSPDAREVKRIVNAQLSAFLPGAPIETFERDVREWRIRYG
                     AYALEEIADGPAVWGATA"
BASE COUNT          963 a         2064 c         2022 g          736 t
ORIGIN      
        1 gccggatcat ccagaacgtg aagaccgcgg gcacgaacaa ccccgtgttc atgctcgacg
       61 agatcgacaa ggtcggcatg gacttccggg gcgacccgtc gtccgccctg ctcgaggtcc
      121 tcgacccgga gcagaacaac agcttccagg acaactacct ggaggtgccg ttcgacctga
      181 gcaaggtgct cttcgtcgcg accggcaacc tcatcgaccc gatcccgatg gcgctccgcg
      241 accggatgga gatcatccag gtcccgggct acacccagcg cgagaaggtc gagatcggga
      301 agcggttcct catccccaag cagatggaga accacggcct caagccgaag aacatcgtct
      361 tcaccgatga ggccatgacc gagctcgtcc aggcgtacac ccacgaggcc ggcgtccgga
      421 acctcgagcg cgagatcgcc aacatcatgc gcaaggtcgc gcggaaggtg gccgaaggcc
      481 gcaaggccaa gacggtcatc gacaacaagc gcctgctcga gttcctcggc ccaccgcgct
      541 tcgagtacgg tgagctggag cccgaggacc aggtgggctc ggcgacgggc ctcgtggtca
      601 ccgaggtcgg tggcgacgtg gtcgcgatcg aggtcacccg catggacggc aaggaggact
      661 tcatcctcac cggccagctg ggcgaggtca tgcgcgagtc ggcccgagcc ggcctgtcgt
      721 ggatccggac ccacgccgcg gagctcggga tcagccgcga gacgttcgag aagcagaccc
      781 tgcacatcca cgttccggcc ggggcgacgc ccaaggacgg cccctcggca ggcatcacga
      841 tggccctcgc gatggtctcg gcgtacaccg gcatcccggt gcgcaaggac ctggcgatga
      901 ccggcgagat caccctccgt ggccgcgtcc tgccgatcgg cgggctgaag tcgaagatcc
      961 tcgccgcgca cctcgccggc gccaggatgg tcgtcatccc gcgcaagaac gagaaggacc
     1021 tccgggacat ccccgacgag atccgcaagc agatcaagat cgtccttgtc gactcgatga
     1081 acgaagtcct cgagacggca ctccggcggc ggcccaagcc gctgccggcc acgccgaagt
     1141 cggcgccggc gacggatccg gtcaaggagc ccgagacgga gcccgctccg atccgacgtc
     1201 cgaacttccc gcccgaccag ccgaccgtgg tcgtccaggg ctgaggcacg gcctatggcg
     1261 agcccggagt tccaggacta ctacaagacc ctcggcgtgc cgaggaccgc gacccaggcc
     1321 gagatcaaga aggccttccg caagctcgcc cgcgactcgc atccggacaa gcatcccggc
     1381 gatgccgcgg ccgagcgccg gttcaagtcg atcaacgaag ccaacgccgt cctgtccgac
     1441 acggccaagc gtgcgaagta cgaccagttc ggtgcggact gggaggccta cgcccgcgcc
     1501 ggcgcatcgg ccggcggtcg tggcggcgcc gacccgttcg ggccgggcag cccgtttgcc
     1561 ggcttcggcg ggttctccgc cggcggcgcg cgcgctggtg ccggcacggg ccccggcggc
     1621 gtccggttcg agttccgctc gaccggcggt gacgccggcg cgttctccga cttcttccac
     1681 atgatgttcg gtgacgaggg ggccgccgcg ggcgagccgg agcggttcgc caggtcaacg
     1741 tccggagaat cgatcgacga cctcctcgcc cgcatgggca ttgccggcgc cggtggcgct
     1801 gcggcaggtg cccgcccacg tgccggcgcg gcccctcggg gcggcgcggc ggcggcaggc
     1861 ccgccgatcg aggtcaccgc cgagatctcg ctcgaagaat cgttccacgg cacgagtcgg
     1921 gtcatcgagc tcgacggacg ccggctcgag gtcaccatcc cgcggggcgc ggacaccggc
     1981 aaccggatcc ggctgaccgg caagggcccg agcggtcgcg acctggtggt cgccatcaag
     2041 ctcaagccgc acaaggtctt cacgcgccgc ggccacgatc tcgagcggga ggtcccggtg
     2101 acgctccgcg aggcgctgct cggcggcgag attcccgtcg ggacccccaa aggcaaggtg
     2161 ctgctgaagg tgcctgcggg ctcgcagaac ggccgcacca tccggctcaa gggccagggc
     2221 atgccccact tcaagggcga cggcacgggc gacctgttcg tccgcgtccg cgttgtcctg
     2281 cccaccgacc tctccgacga ggatgccgct gcggcggcca aggtcctcga caagatccag
     2341 caacccgacc cgcgcgccta ggcgcgtacg agacccacga ggccaaccaa tgcaactcga
     2401 tcacttcacc gagaaggccc aggaggccat tgtcgccgcc cagcagctcg cggggcggct
     2461 cgacagcccg gtgctcgacg ctgagcacct cctctccgcg ctcgtcgagc ccgatgacgg
     2521 cgtgcccgcc gagacccttc gccggctcgg cgtcgacctg cccggcttcc gcggcgaggt
     2581 cgccgcgatc ctcgccaagc gcgcccggat ccagggcggc tcgctggcgc tggactcgcg
     2641 cgccaagcgc gtcatcgatc gcgcccagga ggaggcccgc cgtctcggcg acgagtacac
     2701 ctcgaccgag cacctgctga tcggcatcgc cgagatcggc ggcgaggccc agaagctgct
     2761 cgagcgccac aacgccggca aggaacagct cctcaccgcg ctccagtcgg tgcgtggcgg
     2821 ccagcgcgtg acctcaccga accccgaggg cacctaccag gcgctcgaga agtacggccg
     2881 cgacctcacc gccgaggcgc gcgcgggccg gctcgacccg gtcatcggcc gcgacgacga
     2941 ggtccggcgg gtgatgcagg tcctgtcccg tcgcaccaag aacaaccccg tgctcatcgg
     3001 cgagccgggc gtcggcaaga cggcgatcgt cgagggcctc gcccagcgaa tcgtccgcgg
     3061 cgacgtcccc gagacgctca aggacaagcg cgtcgtcgcg ctcgacctcg gcgcgctcat
     3121 tgccggcgcg aagttccgtg gcgagttcga ggagcggctc aaggccgtcc tgaaggagat
     3181 caaggactcg gcggggcgcg tgatcctgtt catcgacgag ctccacaccg tcgtcggcgc
     3241 gggcgccgcc gagggcgcaa tggacgcctc gaacctgctc aagccgatgc tcgcgcgcgg
     3301 cgagctccat tcgatcgggg cgacgacgct cgacgagtac cgcaagcaca tcgagaagga
     3361 cgccgcgctc gagcgccgct tccagcaggt ctacgtcgac cagccgaccg tcgaggagac
     3421 gatctcgatc ctgcgcggcc tgcgcgagcg ctacgaagtc catcacggcg tgcgaatcac
     3481 cgattccgcg ctggtcgccg cggcgacgct gtcggatcgg tacatcaccg agcgcttcct
     3541 gccggacaag gcgatcgacc tggtggacga gtcggcgtcg cgcctgcgca tggagatcga
     3601 ctcgatgccc gtcgagctcg acgagatcga gcggcgccgg atccagctcg agatcgagcg
     3661 cgaagcgctg cgcaaggaga aggacgacgc ctcgaaggcg cgcctccagg cgctcgagaa
     3721 ggagctggcc gagatcgcgg aggaaggcgg cgccatgaag cagcgctggg agggcgagaa
     3781 ggccgcgatc caggcgatcc gcgcgacgaa gtccgagctc gagggactcc aggtccagat
     3841 cgagcaggcc gagcgcgaga tgaactacga gcgcgccgcg cagctcaagt acgggacgca
     3901 gcgcgagctc cagacgcggc tcgccgggca ggaggccgcg ctcgccgccc tccagggccc
     3961 gaactccctc ctgaaggagg aagtgaccgc ggacgagatc gcggacatcg tcgcgaagtg
     4021 gacgggcatt ccggtgacac ggctcctcga gggcgagctg gccaagctcg tccacatgga
     4081 ggagcggctg cacgagcggg tcgtcggcca ggacgaggcg atcgcggccg tgtccgacgc
     4141 ggtgcgccgg gcccgtgccg ggctcaagga cccgaagcgg cccatcggct cgttcctgtt
     4201 cctcggcccg accggcgtcg gcaagacgga gctggctcgc gcgctcgcgg ccttcatgtt
     4261 cgacgacgag cacgccatga cccgcatcga catgagcgag tacatggaga agttcgccgt
     4321 gtcccgcctg atcggcgcgc cgcccggcta cgtcggctac gacgagggcg gccagctgac
     4381 cgaggcgatc cgccgccggc cataccaggt cgtgctgctc gacgagatcg agaaggcaca
     4441 cccggacgtg ttcaacgtcc tgctccaggt cctggacgac ggccgcctga ccgacagcca
     4501 gggccgcacg gtcgacttca agaacgcgct gatcatcatg accagcaacg tgggctcgca
     4561 ggccatcgcc gccgtcggcg gccggcccgg cgagggcgag gccgaggcct acgaggccat
     4621 gaagcgccag gtcaccgacg ccctccgatt gcagttccgg ccggagttcc tgaaccgggt
     4681 cgatgaggtg atcgtgttcc acgcgctcac cgaggccgac ctcgcggcga tcgtcgagct
     4741 gctcgtcgcc gagctccagc gccggctcgc ggcccaggac ctcgtcctgg agctcacgcc
     4801 ggccgcccgg gcgctcatcg cccgcgaggg caccgacccg acgttcgggg cgcggccgct
     4861 caagcggacg atccagcgac tcgtcgagaa cccgctggcg cgggcgctca tcgagggcag
     4921 cttcaagccg ggccagacgg tccacgtcga tgccgatccg gtgggcacga cgctcgtctt
     4981 cagcgccgac ggccagtccg tcgtcaccga tgcggggaac cggcgcgacg cgcgacggcg
     5041 ctccgatgac gagggcctcg agccggtcgg cgccggctcg agcggcggtc gtgggaagcg
     5101 gcgcggatcc gtcctcgacc tgccggatac cgatcccgaa cggggtgagg gcggcaacgg
     5161 cggcgggggc cgcccgaact gaggtacgcc gaggccgtcg cccgcctcga atcgctgccc
     5221 gagcagctgc cgagcccgcc ggacgcgctc atgcccatcc ggatcgatgg cgcaccttgg
     5281 aagctgccgg ccgacggcat ggattcggca cgaccggcag ccgtgctcgt cctcgtcgcc
     5341 cccgacgagg cgggtgaggc gcgcgtcgtc ctgatcgagc ggaccagcta cgacgggcac
     5401 cactccggcg agatcgcgtt cccgggtggc gcggcggagc caggggatgc atcgctcgtc
     5461 gagacggccc tgcgcgaggc cgccgaggag atcgggctcg agccggccgc gatcgagctc
     5521 cgcgtgatcg gcgagctcga gccgttctgg atcccggtca gcaacttccg ggtgacgccg
     5581 gtgctggccg tcgcgaaccg cctgccggcg tggtcgcccg acgcgcgcga ggtgaagcgg
     5641 atcgtcaacg cgcagctctc ggcgttcctg ccgggtgcgc cgatcgagac gttcgagcgc
     5701 gacgtgcgcg agtggcggat ccgctatggc gcctacgccc tcgaggagat cgcggacggc
     5761 cccgccgtct ggggcgcgac ggcga
//
LOCUS       DATBSW010000229         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_269034, whole genome shotgun sequence.
ACCESSION   DATBSW010000229 DATBSW010000000
VERSION     DATBSW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269034"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIF63_06425"
     CDS             <1..322
                     /locus_tag="VIF63_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY8239051.1"
                     /translation="YADAPRNRQWATTVRVLPLERADSTDDFRTIDDRDELERAFRSI
                     PLDQRAVFVLHHHVGLPLVAVAETLGIPDGTARSRLHYATRALRAAFVAGRAQAIERE
                     GRLA"
     gene            319..1116
                     /locus_tag="VIF63_06430"
     CDS             319..1116
                     /locus_tag="VIF63_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239052.1"
                     /translation="MSDQLFDRAVRDWLEDGSDRTPPASIDAVLLAVKTTPQERDLRI
                     PRRFSPMTTSMRVAAGIALAAIVGGGALLYLNRGPAQGVGGEPTPSPTTLPTPTPFVF
                     TKPSVAPSATPPSTASWIPFTSTQYGYQIAYPPTWEADVTASRDWVLAADRLLSPSDG
                     MNGSADHLIGDGVGGVTAVTGFAADVPTGQSESQWLAAYYADAAFCPTSVPIFQPITV
                     DGHQGRLDQCYDAQAIVFIGDRVYVFAIHRTDNQPLFSSFLSTVRFP"
     gene            1265..1822
                     /locus_tag="VIF63_06435"
     CDS             1265..1822
                     /locus_tag="VIF63_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8239053.1"
                     /translation="MHVALVELARAGDEEAFASLARAAGDRLLAIAYRILRDLGLAED
                     AVQQTLVLAWRELPTLRDVDRFDAWLRRLLVNACYREARRGRQWAANVRVLPNELPAR
                     TDDYNSVVERDQLDRGFRRLPPEQRAVFVFHHYLGLTLLEVADELGVPLGTVKSRLHY
                     ATNTLRAALEADLRLPTDSSREHSA"
     gene            1819..>2135
                     /locus_tag="VIF63_06440"
     CDS             1819..>2135
                     /locus_tag="VIF63_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239054.1"
                     /translation="MNTNADFDRHAAAYLADGPTELSGRVLDAALREVHETRQRRRWS
                     APWRSQPMFSAARLAAVAAVALLAFGFALVGVSGPNPTPSPSTIASAGPSTGCLVAPA
                     NGQL"
BASE COUNT          315 a          791 c          684 g          345 t
ORIGIN      
        1 ttacgcagat gcgccgcgca accggcaatg ggcgacgacg gtccgggtcc tcccgctcga
       61 gcgagcggac tcgaccgacg acttccgaac catcgacgat cgcgacgagc tcgagcgggc
      121 cttccgctcg atcccgctcg atcagcgagc ggtgttcgtc ctgcaccacc acgtgggcct
      181 gcccctcgtt gcggtagccg agacgctcgg catcccggac ggcaccgcgc gatcgcgact
      241 gcattacgcg acccgcgccc tgcgtgccgc cttcgtggcg ggtcgagcgc aggccatcga
      301 gcgagaagga cgcctggcat gagcgaccaa ctgttcgacc gcgccgttcg tgactggctg
      361 gaggacggtt ccgaccggac cccgccggcc tcgatcgatg ccgtcctgct cgctgtcaag
      421 acgaccccac aggagcggga tctccggatc ccgcggaggt tcagcccaat gacgacatcg
      481 atgcgggtcg ccgccgggat cgcgcttgcc gcgatcgtgg gcggcggtgc cctgctgtac
      541 ctgaatcgcg gcccggccca gggcgtcggc ggcgagccga caccgtcgcc cacaaccctg
      601 cccaccccga cgccgttcgt cttcacgaag ccgtccgtgg caccctcggc gaccccgccc
      661 agcacggcca gctggatccc cttcacctcg acccaatacg gctatcagat cgcgtaccca
      721 ccgacgtggg aggcagatgt cactgcgtca cgggactggg tgctcgctgc agatcgcctc
      781 ctgagcccaa gcgacgggat gaacgggtcc gccgatcacc tcatcggtga tggggtcggc
      841 ggggtgaccg ccgtcacggg ctttgcagcg gacgttccca ccgggcagtc ggagagtcag
      901 tggctcgccg cgtactacgc ggatgcggcg ttctgcccga cgagcgtccc gatcttccag
      961 ccgatcacgg tcgacggcca ccagggccgg ctcgaccagt gctacgacgc acaggccatc
     1021 gtcttcatcg gcgatcgcgt gtacgtcttc gcgatccacc gaaccgacaa ccaaccgctc
     1081 ttcagctcgt tcctgtcgac ggtcaggttc ccgtaaagcg ccgtcttcgg acgcctccgt
     1141 gctcgctctg cgggcgcgga ggcgccttct ttcgatcccc atgaccaaca tcgaaccatt
     1201 cgacggcgac gggtctcatc tacggtaggc gtccaggtcg gacgcatcgc cgaagggagc
     1261 ccgggtgcac gttgccctcg tcgagctggc gagagccgga gatgaggagg cgtttgccag
     1321 cctggcacgt gcggctggtg atcggctcct cgcgatcgcg tatcggatcc tgcgcgatct
     1381 cgggctcgcg gaggatgccg tccagcagac cctcgtgctc gcctggcgag agctgccgac
     1441 gctccgggat gtcgatcgct tcgacgcctg gctgcgccgg ctcctcgtca acgcgtgcta
     1501 tcgggaggcg cgtcggggcc gccagtgggc ggcgaacgtc agggtcctgc cgaacgagct
     1561 tccagcgcgg acggatgact acaactccgt cgtcgagcgc gaccagctcg atcgtggatt
     1621 ccggcgcttg ccgccggagc aacgcgccgt cttcgtgttc caccactacc tcggcctgac
     1681 gctgctcgag gtcgccgacg agctcggcgt gccccttggg actgtcaagt cgcggctcca
     1741 ctacgccacc aacacgctgc gcgccgccct cgaggcggac cttcgcctgc ccacagattc
     1801 ctcgcgggag cattcagcat gaacacgaac gccgacttcg atcgacacgc cgcggcctac
     1861 ctggccgacg gcccgaccga gctgtccggg cgcgtgctcg acgccgccct ccgcgaggtc
     1921 cacgagacac gacaacggcg ccgctggtcg gccccgtgga ggtcccagcc gatgttctcc
     1981 gccgcccgcc tggccgccgt tgcggccgtt gcgctccttg ccttcggttt cgccctcgtc
     2041 ggcgtctccg gcccgaaccc gacgccgtca ccttccacga tcgcgagcgc cggcccgtcg
     2101 acaggatgcc tcgttgctcc ggccaacggg cagct
//
LOCUS       DATBSW010000230         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_691900, whole genome shotgun sequence.
ACCESSION   DATBSW010000230 DATBSW010000000
VERSION     DATBSW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691900"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..900)
                     /locus_tag="VIF63_06445"
     CDS             complement(19..900)
                     /locus_tag="VIF63_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY8239055.1"
                     /translation="MPLTPDSAGLAGRVRLGNPANGNAPRPNGACRVLMIGDVIGKPG
                     RLAVEALLPQLRDERSIDFVTANGENLAGGMGLTASTADALFAVGVDVITSGNHIWDK
                     KEIYPRLESDDRILRPLNYGTTGVPGRGWGVYHAHDGSELAVINLQGRTYMQPIENPF
                     LEADRLLEEAGEPLPPIRLVDFHCEITSEKNALGVHLDGRVSAVVGTHTHVPTADDRI
                     LPSGTAYQSDLGCTGGAWSVIGFNPATVLPRFLNGLPTRFEVAEGPVIFNATQIDVDP
                     ATGRALGIERIQRIWEG"
     gene            1114..1935
                     /locus_tag="VIF63_06450"
     CDS             1114..1935
                     /locus_tag="VIF63_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239056.1"
                     /translation="MSVIITLVVLLGALTTATAAAVPPERDSSGASAGGQDKGGGGGG
                     GGAPKPARGYDISYPQCGAAYPANPLFAIVGVNGGRVFSGNSCLVSQLAWGGDARAEL
                     YVNTGNPGPALSSFWPRGQTTPRFCDANNPDTADCAYDYGWNAAADSYTRTEQAYAAR
                     SIAANPNATAWWLDVETSNSWRSSTALNVAALQGGFDYLRSRGVTRLGFYSTTVQWGT
                     ITGGTRVFAPSPSWGAGAPSDKAAKQHCISTPGFTGGRLAMVQYPYAGFDANVRC"
     gene            complement(1932..2468)
                     /locus_tag="VIF63_06455"
     CDS             complement(1932..2468)
                     /locus_tag="VIF63_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239057.1"
                     /translation="MGRLLSGIVVAAILVVACGPDPSTPSPQVTSAQSGTPVASEAAT
                     LAVTGAGVLCGPWWMGCSAYLAVEPPDWSLPEAWAPTADDIWFETDLRAGRETTPITG
                     VRQAGRDRIEPGAYRVVVIKTLSPDTQPEGTFEASVLCMSDVEIPPGTRSVRVDVEFG
                     AACMITTSLDDSTGAPGP"
BASE COUNT          389 a          892 c          862 g          387 t
ORIGIN      
        1 tgaccgcgct ggtcgggctc atccctccca gatccgctgg atgcgctcga tgcccagcgc
       61 ccgcccggtc gctgggtcga catcgatctg ggtcgcattg aagatcaccg ggccctcggc
      121 gacctcgaac cgcgtcggca agccgttcag gaaccgcggt aggaccgtgg ccgggttgaa
      181 cccgatgacc gaccacgcgc cgcccgtgca accgagatcg ctctggtacg cggtcccgct
      241 cggcaggatg cgatcgtcag ctgtgggcac gtgcgtgtgc gttcccacga ccgcggaaac
      301 acgcccgtcg agatgcacgc cgagcgcgtt cttctcggac gtgatctcgc agtggaagtc
      361 gaccaggcgg atcggcggca agggctcgcc ggcctcctcg agcagccgat cggcctcgag
      421 gaatgggttc tcgatcggct gcatgtacgt ccggccctgg aggttgatca ccgccagctc
      481 ggagccatcg tgggcgtggt agacgcccca acctcggccc gggacgccgg tcgtgccgta
      541 gttgagcggt cggaggatcc gatcgtcgga ctccaggcgc gggtagatct ccttcttgtc
      601 ccagatgtgg ttgccggagg tgatgacgtc gacgccgacc gcgaacaggg cgtcggcagt
      661 cgatgccgtc aggcccatcc cgccggcgag gttctcgccg ttggcggtga cgaagtcgat
      721 cgagcgctcg tcgcgaagtt gcggcagcag ggcttccacg gccaggcgcc cgggcttgcc
      781 gatgacgtcg ccgatcatca ggacgcggca cgcgccgttc gggcgtgggg cgttgccgtt
      841 tgccgggttg ccgagccgga cgcggccggc aaggcccgcg gaatcaggtg tcaggggcac
      901 ctcgggaggc tagcacccgg ttcccgtcgg agcgcggcgc gcctcggccg acgaccaccg
      961 tattgcaaga ccggtgaatc accgcaccgc caatccgtcg cttcctgact gacaggatcg
     1021 acgcattggg gtacggtgag ggtcgcccaa acccccttac cgcccgcgag tctgtgccca
     1081 gtgcgtatga gaaccctcca tcctgctgcc cgcgtgagcg tgatcatcac gctcgtcgtg
     1141 ttgcttggcg cgctgacgac ggccacggcg gcagcggtgc cgcccgagcg cgacagctcg
     1201 ggcgcgtccg caggcggcca ggacaaggga ggcggtggcg gtggcggagg cgcacccaag
     1261 ccggcccgcg gctacgacat ctcctacccc cagtgcggcg cggcctatcc ggccaatccc
     1321 ctgttcgcga tcgtcggcgt gaacggcggc agggtgttca gcggcaactc gtgcctcgtc
     1381 tcgcagcttg cctggggcgg cgacgctcgt gccgagctgt acgtcaacac cggcaacccc
     1441 gggcctgcgc tctcgagctt ctggcctcgc ggccagacca cgccacgctt ctgcgacgcg
     1501 aacaaccccg acactgccga ttgcgcctac gactacggct ggaacgcggc cgcggactcg
     1561 tacacgcgca ccgagcaggc ctacgcggcg cggagcatcg ctgccaaccc gaacgccacg
     1621 gcctggtggc tcgatgtcga gacctcgaac agctggcgca gcagcacggc gctcaacgtg
     1681 gccgcgctcc agggcggctt cgactacctg cgctcccgcg gcgtgaccag gctcggcttc
     1741 tactcgacga ccgtgcagtg gggcacgatc accggcggga cgcgggtctt cgctccctcc
     1801 ccgagctggg gcgccggcgc cccgagcgac aaggccgcga agcagcattg catttcgacg
     1861 cctggcttca cgggcggccg gctcgccatg gtccagtacc cgtacgcagg attcgacgcg
     1921 aacgtccgct gctaggggcc aggggcgcct gtcgagtcgt cgagggacgt cgtgatcatg
     1981 cacgcggctc cgaactcgac atcgaccctg actgagcgag tccccggcgg aatctccacg
     2041 tcggacatgc acaggaccga cgcctcgaac gtcccctcgg gctgcgtgtc gggtgagagc
     2101 gtcttgatca ctacgacccg gtaggcgccc ggctcgatcc gatcccgacc cgcctgcctg
     2161 acgccagtga tcggtgttgt ctctcggccg gcgcgcagat cggtctcgaa ccagatgtca
     2221 tcggccgtcg gagcccaggc ttccggcagg ctccaatctg ggggctcgac cgcgaggtac
     2281 gcgctgcaac ccatccacca tgggccgcac aggacaccgg cgcccgtaac ggccagtgtc
     2341 gccgcctcgc ttgctacggg cgtgccggac tgggcggagg tcacttgtgg cgatggcgtc
     2401 gacggatccg ggccgcatgc cacgaccagg atggcggcga ccacgatccc cgacagcagg
     2461 cgccccatca cgaccagccc agatcagtgg cggcggcgaa ccgagaccgc tacttggcgt
     2521 actcgacggc
//
LOCUS       DATBSW010000231         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_92331, whole genome shotgun sequence.
ACCESSION   DATBSW010000231 DATBSW010000000
VERSION     DATBSW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92331"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1415
                     /locus_tag="VIF63_06460"
     CDS             63..1415
                     /locus_tag="VIF63_06460"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8239058.1"
                     /translation="MVAPYEAVSGVEQIEQAKARGIRFIQLQFTDIIGVVKAVTIPVH
                     QLEGSVRHGTWFDGSSVEGFTRIAESDQYLMPDMRTFSEIPWSNGTAARVICDVFTPR
                     GEPFAGDPRFVLRRQVERARKLGYIVNMGPELEFFLFRRAEDGTIEPLPHDQAGYFDF
                     SSDLATTIRHDMVDALDAFGIRVEAAHHEVAAGQHEIDFEYADALQTADNAITFKFTL
                     KAIAQQHGLYATFMPKPIHGINGSGMHTHQSLYSIDASRNAFADKDSSYGLSPLAKSY
                     AAGILAHARGMIAVLAPLVNSYKRLVPGYEAPTYLTWGRTNRSALIRVPMVSPGKSVE
                     GTRLEVRCPDPSSNTYLAFAAMIAAGLDGVEKGMELSDPVEESLFEMDATQIAAKSIK
                     ELPGTLGEALDELEKDDVIREALGDHVFSHFVDAKRAEWDEYRTQVSDWEVERYLEQF
                     "
     gene            complement(1447..>1781)
                     /locus_tag="VIF63_06465"
     CDS             complement(1447..>1781)
                     /locus_tag="VIF63_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239059.1"
                     /translation="GILPELMRATLANPPEVDFNTLAPNRMGLATEYRHEAERQGFDP
                     TIDPDLVFDILFGAGLAHLFATGTPPSREYVSQLATILTEGLRARPRTAGTKRVHSKA
                     AGKSRPPR"
BASE COUNT          302 a          620 c          579 g          280 t
ORIGIN      
        1 gacgagctct ggccgctgcc gaagtatcgc gagctcctct tccagtacta ggagggacag
       61 ccatggttgc gccctacgag gcggtgtcag gcgtggaaca gatcgagcag gccaaggcca
      121 ggggcattcg gttcatccag ctccagttca ccgacatcat cggtgtcgtc aaggccgtca
      181 cgatcccggt ccatcagctc gaaggttcgg tgcgccacgg gacatggttc gacggctcgt
      241 ccgtcgaggg cttcacccgc atcgccgaga gcgaccagta cctgatgccc gacatgcgga
      301 ccttctcgga gatcccgtgg tcgaacggaa ccgcggcccg ggtcatctgc gacgtcttca
      361 cgccccgcgg cgagccgttc gcgggcgatc cccgcttcgt cctccgccgc caggtcgagc
      421 gggcacgcaa gcttggctac atcgtcaaca tgggcccgga gctcgagttc ttcctgttcc
      481 gccgcgccga ggacgggacg atcgagccgc tgccccacga ccaagccggc tacttcgact
      541 tcagctccga cctggccacg acgatccgcc acgacatggt cgacgccctc gacgcgttcg
      601 gcattcgcgt cgaggcggcc caccacgagg tggcggccgg ccagcacgag atcgacttcg
      661 agtacgccga cgccctccag accgcggaca acgcgatcac cttcaagttc acgctcaagg
      721 cgatcgccca gcagcacggc ctgtacgcga cgttcatgcc caagccgatc cacgggatca
      781 acggctccgg catgcacacc caccagagcc tgtactcgat cgacgcgtcc cggaatgcgt
      841 tcgccgacaa ggactcgtcg tacggcctct cgccgctcgc caagtcgtac gcggcgggca
      901 tcctcgcgca cgcccggggc atgatcgcgg tcctcgcgcc gctcgtgaac agctacaagc
      961 ggctcgttcc gggctacgag gcgccgacgt acctgacctg gggccggacg aaccgctcgg
     1021 ccctcatccg ggtgccgatg gtctcgcccg gcaagtcggt cgagggcacc cgcctcgagg
     1081 tccgctgccc ggatccgtca tcgaacacgt acctcgcctt cgcggcgatg atcgcggccg
     1141 gcctcgacgg cgtcgagaag ggcatggagc tcagcgatcc ggtcgaggag tcgctcttcg
     1201 agatggacgc gacgcagatc gcggcgaagt cgatcaagga gctcccgggc acgctcggcg
     1261 aggccctcga cgagctcgag aaggacgacg tgatccgcga ggcgctgggc gaccacgtct
     1321 tcagccactt cgtcgacgcg aagcgcgccg agtgggacga gtaccgcacg caggtctccg
     1381 actgggaagt cgagcgatac ctcgagcagt tctaggcaaa cgctgcgggc aacactcgcc
     1441 ggcagatcag cgaggcggcc gggatttccc ggccgctttc gaatgaaccc gcttcgtccc
     1501 tgcggtccgc ggtcgcgccc tgagcccctc ggtcaggatc gtcgcgagct ggctgacgta
     1561 ctcgcgagac ggcggggttc cggtcgcgaa caagtgggcg agcccggccc cgaacaggat
     1621 gtcgaagacc aggtccggat cgatcgtcgg gtcgaagccc tgtcgctccg cctcgtgtcg
     1681 gtattccgtc gcaaggccca ttcggttcgg ggcaagcgtg ttgaagtcga cctcgggcgg
     1741 attcgcgagg gttgcgcgca tgagctccgg caggatcccc g
//
LOCUS       DATBSW010000232         7316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_92352, whole genome shotgun sequence.
ACCESSION   DATBSW010000232 DATBSW010000000
VERSION     DATBSW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92352"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VIF63_06470"
     CDS             complement(<1..1104)
                     /locus_tag="VIF63_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY8239060.1"
                     /translation="MTALEPAVDRTTEPATAQPATLRVLVAESIAPEGIDALRAEHEV
                     DVRTGLSAEALREIVGDYDALVVRSQVAVDAALIAAGPRLQVIGRAGVGVDNVDLDAA
                     TRAGITVVNAPTGNTIAAAEHTIALLLALARRVPAADASIRRGEWVRGPLQGVQLRGR
                     TLGIVGLGKIGMAVAERARGLGMTLLGSDPYVTSEQALLRGVELVTFEELIARSDAVT
                     VHVPLSRSTTGLLNTKTIARMKPGAFVLNVARGGIVDEAALAAALRDGRLGGAGIDVF
                     EAEPPTGSPLLDAPNTVLTPHLGASTAEAQVAVAEEIAEQVLDVLAGRPARYAVNAPL
                     LSAEAEQTIGPYLPLAETLGRFLAQFARAGVGTF"
     gene            complement(1101..2264)
                     /locus_tag="VIF63_06475"
     CDS             complement(1101..2264)
                     /locus_tag="VIF63_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY8239061.1"
                     /translation="MTRSEPNLRIPGPTSLPPSVKEAGGRQMINHRGPEFKELLGRLQ
                     AGMKPYFGTTGDIAILSCAGTGGLEAAVVNTLSPGDRVLGVSIGAFGDRFAKIAEAYG
                     ASVDRISAEWGWAAEPDEVRERLGGGDYKAVLLTHNETSTGVMNPIPELAAAVREAAP
                     SALILVDTVSSLGAVPFDMEGWGIDVVVTGSQKAWMAAPGLAMVAASPRARAAMETAK
                     MPRFYLDLKRHFDAMADGETPWTPAIAVAFQVDEGIRLMHEEGQAGVFARHIACAAAT
                     QSGLEALGFQLFAEPTHRSRTVTAAWIPDGLDWKAFNGELKRRQLVLAGGQGKLTGKI
                     FRLGHLGSVTLEEILDALSTIEAVSLEWGREVTPGAAVAAAERAAADAALQPA"
     gene            2461..3492
                     /locus_tag="VIF63_06480"
     CDS             2461..3492
                     /locus_tag="VIF63_06480"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8239062.1"
                     /translation="MDTILRRLTGLALGAALAFGPAVTAVRAATIANDDAYEMNENGT
                     LVIDAPGVLANDESDGGAAICVAGYDYPGTEGHVETWGTDGSFTFTPWEHWNGTTSFV
                     YGMKAGDGQCIGQADDQGLVIVTVHPVNDQPTAVVQGGTCDDGVTVEQDSGPFDDAAH
                     CVENHNWGQSLDELTQLVDEWVVTTDRPDLFFDKPSIAIFQTTFGRLSFTPAPGAHGT
                     ATVTVRSRDNGGTARGGDDLSPPVEFTITITKTPTPEPTQAPPEPTETPTEAPSDAPS
                     AEPSASDGGVATESPSMAPTEPPIGDPTGAIVGTVPWALLSIVALAGVLTFGGGVLLP
                     RYLGKRRSP"
     gene            3543..4535
                     /locus_tag="VIF63_06485"
     CDS             3543..4535
                     /locus_tag="VIF63_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8239063.1"
                     /translation="MGAGRVLAATTAVDDPDYTVDEDFDGGLVVPAPGVLANDTGDGG
                     TLCVTDYDATGLRGSLGALNIDGSFTFTPEPNFNGVTTFTYGLGEFPPLGECPQTADV
                     RGVVTITVAPVNDPPTATADSFIVLAGRTLNVGEPGVLINDSDIDGDTITAVKVKSPT
                     HGVLNLEADGSFSYTPTAGFTGNDGFSYRASDGSPDMSPERAVSLHVTPIPPSPTPKP
                     SPTPVPTPVPTPTPVPATPTPEPSLEPSPSPSPSASLEPSTAPSASPAPSPSIVVLPS
                     PTAQPGPASDEDGVSTPVLIVVVLFAILLAFGAAVYVPKLLRGPGTDADDDDVS"
     gene            complement(4628..5035)
                     /locus_tag="VIF63_06490"
     CDS             complement(4628..5035)
                     /locus_tag="VIF63_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239064.1"
                     /translation="MAALLIVCVVGAGAGYYVMTGGHLFANIPPEGVIWFGASFDSDT
                     YDVRDQLTSIGPYDEFVMVGRLPRSIGGDRIAIRGYLDDALIMVAWTESTDEGGIWGF
                     NIGPMGKPGNWKFEIAEVGGNALASGQFLVVPE"
     gene            5214..6095
                     /locus_tag="VIF63_06495"
     CDS             5214..6095
                     /locus_tag="VIF63_06495"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY8239065.1"
                     /translation="MTDARQDQVDVVVAADCELGEGPVWDARSGRIVWIDIPGRRVHL
                     HDPTTGATESMATPLDIGAIVPRRAGGFMAALQDGFWILGDGPARRVATVPEGRPEIR
                     FNDGKCDPQGRLWAGTMPYDEVSPVGCLYRLDRDGSVARILDGVTISNGLAWSGDGTT
                     MYYIDTPTRRVDAFSYDAATGDIGDRRVAIEIPESAGWPDGMAIDVEDGLWVALWGGS
                     AVHRYVEGRLDRVVALPVSQPTSCAFGGPNLDELYITSAWTGLDPAARRDEPLAGGLF
                     RIRPGVRGPAAPSYDGI"
     gene            complement(6104..7018)
                     /locus_tag="VIF63_06500"
     CDS             complement(6104..7018)
                     /locus_tag="VIF63_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY8239066.1"
                     /translation="MKLGFLTAPFPETPLTEVADWAASAGFEVLEIACWPRGDGAARR
                     YAGTSHIDVANLSASQARDIRAEVEAKGLSISGLGFYPNPLNPDPEHRATVIAHLKHV
                     IDAAEKMDVPLVNTFMGGDRAKTQDENWEEALRVWPDIVRYAQDHGRRITLENCPMLF
                     SNDEWPGGLNIATTPRMWRRILEQWEGTIGLNFDPSHLVLQMIDMRRFLREFGKHVLH
                     YQAKDLLIDRDGLYERGVFSMGMGWQIPRIPGLGDVDWGLVHGELYLAGYEGDCIIEH
                     EDRRFEGTDAKVKQGFLIARDVLRPLCH"
     gene            complement(7047..>7316)
                     /locus_tag="VIF63_06505"
     CDS             complement(7047..>7316)
                     /locus_tag="VIF63_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239067.1"
                     /translation="RDEPNELLLRNPALMNRAGAAAAYLPGGHAEGFADTFKAAFAAI
                     YADVSAGGPSASPAYATFADGHDEMLVNDAIAESARSNTWAEVAR"
BASE COUNT         1064 a         2619 c         2535 g         1098 t
ORIGIN      
        1 gaacgtgccg acgcccgccc gcgcgaactg ggcgaggaac cggccgagcg tctcggccag
       61 gggcaggtac ggcccgatcg tctgctcggc ctcggcggac agcaggggag cgttgaccgc
      121 gtagcgtgcc ggccggccgg caaggacgtc gagcacctgc tcggcgatct cctccgcgac
      181 cgcgacctgg gcctccgcgg tcgacgcgcc gaggtgcggg gtcaggacgg tgttgggcgc
      241 gtccagcaac ggcgagccgg tcgggggctc cgcctcgaag acgtcgatcc cggcgccgcc
      301 aaggcggcca tcgcgcagtg ccgccgccag cgcagcctcg tcgacgatcc cgccgcgagc
      361 gacgttcagg acgaacgcgc cgggcttcat cctggcgatc gtcttcgtgt tgagcagccc
      421 ggtcgtcgag cggctcaggg gcacgtggac cgtgaccgcg tccgatcgcg cgatcagctc
      481 ctcgaacgtc acgagctcca cgccccgcag gagcgcctgc tcggaggtga cgtacggatc
      541 gctgccgagg agcgtcatcc cgaggccgcg cgctcgctcc gcgacggcca tgccgatctt
      601 gccgaggccg acgatgccga gcgtccgccc gcggagctgc acgccctgga gcgggccgcg
      661 gacccattcg ccgcgccgga tcgacgcgtc ggcggcgggg acgcggcggg ccagggcgag
      721 gagcagcgcg atcgtatgct cggcggccgc gatcgtgttg ccggtcggcg cgttgacgac
      781 ggtgatcccg gcccgcgtcg ccgcgtccag gtcgacgttg tcgacaccca cgccggcccg
      841 gccgatgacc tggagtcgcg gacccgcggc aatgagtgcc gcgtcgaccg cgacctggct
      901 ccggacgacg agcgcgtcgt agtcgccgac gatctcccgc agcgcctccg cggacagccc
      961 ggtgcggacg tcgacctcgt gctccgcgcg cagcgcgtcg atgccttcgg gcgcgatgga
     1021 ctcggcgacg aggacccgca gcgtcgcggg ctgcgcggtg gcgggctccg tggtgcggtc
     1081 gacggcgggc tcgagggcgg tcaagccggc tggagcgcgg cgtcggccgc ggcacgctcg
     1141 gctgccgcga ccgcagcccc gggcgtgacc tcgcgtcccc actcgagcga gacggcctcg
     1201 atggtcgaga gcgcgtcgag gatctcttcg agcgtcaccg agccgagatg acccagccgg
     1261 aagatcttgc cggtgagctt gccctgaccg cccgcgagga cgagctggcg gcgcttgagc
     1321 tccccgttga atgccttcca atcgagccca tccgggatcc acgctgcggt gacggtcctg
     1381 gaccggtggg tcggctcggc gaagagctgg aagccgaggg cctcgagccc ggactgcgtt
     1441 gctgcggcgc aggcgatgtg gcgggcgaag acgccggcct gcccttcctc atgcatgagc
     1501 cggatgccct cgtcgacctg gaacgcaacc gcgatcgcgg gggtccacgg cgtctcgccg
     1561 tcggccatcg catcgaagtg gcgcttgagg tcgaggtaga agcgcggcat cttcgccgtc
     1621 tccatggcgg ctcgggcacg cggcgacgcg gcgaccatcg ccaggccagg cgcggccatc
     1681 cacgccttct gcgacccggt cacgacgacg tcgatgcccc agccctccat gtcgaacggc
     1741 accgcgccca gcgacgagac ggtatcgacg aggatcagcg cgctcggcgc cgcctcgcgg
     1801 accgccgcgg ccagctccgg gatcgggttc atgaccccgg tcgaggtctc gttgtgggtc
     1861 agcaggacgg ccttgtagtc gccgccgccg agtcgctcgc ggacctcgtc gggctcggcc
     1921 gcccagcccc actcggccga gattcggtcg acgcttgctc cgtacgcctc ggcgatcttc
     1981 gcgaaccggt caccgaacgc gccgatcgat acgccgagca cccgatcgcc cggcgacagc
     2041 gtgttgacca ccgctgcctc gagcccaccc gtgccggcgc aggacaggat cgcgatgtcg
     2101 cccgtcgtcc cgaaataggg cttcatcccg gcctgcagcc ggcccagcag ctccttgaac
     2161 tcgggcccgc ggtggttgat catctgccgc ccgccggcct ccttgacgct cggcggcagc
     2221 gaggtcgggc cggggatgcg caggttcggc tcggatcggg tcaacggagc ggcctccagg
     2281 cgtttgggac ggattcggcg acgcccggat ggtcgcacga tggagccccg agcgtcattg
     2341 ggcgtctcgg ttacacgcct gtttcaggcg cgacacctgc cgacggcttg tcgacgccca
     2401 tcctgccgct ggctatgctg cccgaccgga tgggcaggcc gcccgaccga ggtgctggga
     2461 gtggacacga tccttcgacg actgaccggg ctcgcgctcg gggctgcgct cgccttcggg
     2521 cctgccgtga ccgcggtgcg cgccgcgacg atcgccaacg acgacgccta cgagatgaac
     2581 gagaacggca cgctcgtcat cgacgcgccg ggcgtgctcg ccaacgacga gagtgacggc
     2641 ggcgccgcca tctgcgtggc cggctacgac tacccgggca ccgagggcca cgtggagaca
     2701 tgggggacgg atgggtcgtt cacgttcacg ccgtgggagc actggaacgg cacgacgtcc
     2761 ttcgtctacg gcatgaaggc cggcgacggc cagtgcatcg gccaggccga tgaccagggg
     2821 ctcgtgatcg tgaccgtcca cccggtgaac gaccagccga cggccgtcgt tcaaggtggg
     2881 acgtgcgacg acggcgtcac cgtcgagcag gattccgggc cattcgatga cgcggctcac
     2941 tgcgtcgaga accacaactg gggccagtcg ctcgatgagc tcacccagct ggtcgacgaa
     3001 tgggtcgtca ccacggaccg gccggacctc ttcttcgaca agccttccat cgcgatcttc
     3061 cagaccacgt tcgggcggtt gtcgttcacg ccggcgccgg gcgcgcacgg gaccgcgacc
     3121 gtgacggtcc gctcccgcga caatggcggc accgcccgtg gcggcgacga cctcagccca
     3181 cccgtggagt tcacgatcac gatcacgaag acgccgacgc ccgagccgac gcaggccccg
     3241 ccagagccga ccgagacgcc gaccgaagca ccctccgatg caccgtcggc cgaaccctcg
     3301 gcgtcggatg gcggggtcgc gaccgaatcg ccgtcgatgg cgccgaccga gccgcccatc
     3361 ggtgatccga ccggggcgat cgtgggcacc gtgccctggg cgctgctgtc catcgtcgcc
     3421 ctcgccggcg tgctcacctt cggcggcggc gtgctcctgc cgcgctacct ggggaagcgc
     3481 cgatcgccct gatccgccgg ctcccggcat tcctgttcgc cgcgctgctc gcgctcggcc
     3541 ccatcggggc cgggcgcgtt ctcgctgcta cgaccgccgt cgacgaccct gactacacgg
     3601 tcgacgagga tttcgacggc ggcctcgtcg tccccgcgcc cggcgtgctg gccaacgaca
     3661 cgggcgatgg cggaaccctg tgtgtcaccg actacgacgc caccggactc cgcgggtccc
     3721 tgggcgcgct gaacatcgac ggatcgttca ccttcacacc agagccgaac ttcaatggcg
     3781 tgacgacgtt cacgtacggc ctcggcgaat tcccgccatt gggcgaatgc ccgcagaccg
     3841 cagacgtgag gggagtcgtc acgatcacgg tcgcccctgt gaacgacccg cccacggcca
     3901 cggccgacag cttcatcgtc ctcgccggcc ggacgctcaa cgtcggtgag ccgggtgtcc
     3961 tcatcaatga cagcgacatc gacggcgaca cgatcaccgc ggtgaaggtc aagtcaccga
     4021 cgcatggcgt cctcaacctc gaggccgacg gctcgttcag ctatacgccg actgcagggt
     4081 tcaccggcaa cgacgggttc tcgtatcggg cctccgatgg gtcgcccgac atgagcccgg
     4141 aacgggcggt gagcctccac gtgacgccga tcccgcccag cccgacgccg aaacccagcc
     4201 caacgcctgt cccgacgccg gtaccgacgc cgacgcccgt tccggccacg ccaacacccg
     4261 agccgagcct ggagccctcg ccgtcgccct cgccctcggc gtccctcgag ccgtcgactg
     4321 ccccgagcgc cagcccggcc cccagtccct cgatcgtcgt gctgcccagc ccgacggcgc
     4381 agcctggtcc cgcgagcgac gaggacggcg tgtcgacgcc ggtgctcatc gtggtcgtcc
     4441 tgttcgcgat cctgctcgcg ttcggcgcgg cggtgtacgt gccgaagttg ctgcgcggcc
     4501 ctgggacgga cgccgatgac gacgacgtct cgtgatcgat cgatcgaccg tcgacgggtt
     4561 ctgggatctg ttcctcgagg tcctcgcgac gtatcgctag ggcgtccagg gcgccgctac
     4621 gcgggggtca ctccgggacc acgaggaact ggccggatgc cagcgcattg cccccgactt
     4681 cggcaatctc gaatttccaa ttgccgggct ttcccatcgg gcctatgttg aagccccaga
     4741 tgccgccttc gtcggtgctt tccgtccagg caaccatgat cagcgcgtcg tcgagatagc
     4801 ctcggatcgc aatccgatcc ccgccaatgg atcgcggcag ccggccgacc atgacgaact
     4861 cgtcgtaggg cccgatggag gtcagctggt cgcgaacgtc gtaggtgtcc gaatcgaagc
     4921 tcgctccgaa ccagatgacg ccttccggcg ggatgttcgc gaacaggtgg ccaccggtca
     4981 tgacgtagta gcccgctccg gcgccgacga cacagacgat cagcagggcg gccaaccatc
     5041 tgcgtctgct cttccgcgcc ggcgcgtccg gcggcggctc agggaaggag ccatctggat
     5101 caggtagtgg ggccgcggga tcgtcaacct cggactcgga cgatgtgttc cccagcatca
     5161 tgggagcata gatcggcccc acccaacggc cgtatccagc cgggagtcga tcgatgaccg
     5221 acgcgcgcca ggaccaggtg gacgtcgtcg ttgcggccga ttgcgagctc ggcgagggac
     5281 cggtctggga cgcccggtcc ggtcggatcg tctggatcga catccccggc cggcgggtcc
     5341 acctgcacga tccgacgacg ggcgccaccg agtcgatggc gaccccgctc gacatcggcg
     5401 cgatcgtgcc ccgtcgggcc ggcggcttca tggccgcgct ccaggacggg ttctggatcc
     5461 ttggcgatgg tcccgcgcgg cgcgtggcga cggttcccga aggccgaccg gagatccggt
     5521 tcaacgacgg caagtgcgac ccacaggggc ggctctgggc cggcacgatg ccctacgacg
     5581 aggtgtcgcc cgtgggctgt ctgtaccggc tcgatcgcga cggcagcgtc gcccgcatcc
     5641 tcgatggcgt cacgatctcg aacggcctcg cctggagcgg cgacggcacg acgatgtact
     5701 acatcgacac cccgacccgg cgggtcgacg cgttctcgta cgacgctgcg acgggcgaca
     5761 tcggggaccg ccgcgtcgcc atcgagatcc ccgagagtgc cgggtggccc gatggcatgg
     5821 cgatcgacgt cgaggacggg ctgtgggtcg cactctgggg cggcagcgcc gtgcaccgct
     5881 acgtcgaggg tcggctcgac cgtgtcgtcg cgctgcccgt cagccagccg acgagctgcg
     5941 ccttcggcgg gccgaacctc gacgagctgt acatcacgtc ggcgtggacg gggctcgatc
     6001 ccgcagcgcg ccgcgacgag ccgctcgccg gtggcctgtt ccgcatccga cccggcgttc
     6061 gcggccccgc ggccccctca tacgacggga tctgaccagc ccatcagtgg cataggggtc
     6121 ggaggacgtc gcgtgcgatg aggaagccct gcttgacctt ggcgtccgtt ccctcgaagc
     6181 gccggtcctc gtgctcgatg atgcagtcgc cctcgtagcc cgccaggtac agctcgccgt
     6241 ggacgaggcc ccagtccacg tccccgaggc cggggatccg cgggatctgc cagcccatgc
     6301 ccatgctgaa cacgccgcgc tcgtacaggc cgtcgcggtc gatcaggagg tcctttgcct
     6361 ggtaatgcag gacgtgcttg ccgaactcgc gcaggaaccg ccgcatgtcg atcatctgga
     6421 ggaccaggtg gctcgggtcg aagttgagcc cgatcgtgcc ctcccactgc tccaggatcc
     6481 gccgccacat gcgcggcgtg gtggcgatgt tgagcccgcc cggccactcg tcgttgctga
     6541 acagcatcgg gcagttctcg agcgtgatcc tgcggccgtg atcctgcgcg tagcggacga
     6601 tgtccggcca gacgcggagc gcctcttccc agttctcgtc ctgggtcttc gcgcggtcgc
     6661 cgcccatgaa cgtgttcacg agcgggacgt ccatcttctc ggccgcgtcg atcacgtgct
     6721 tgaggtgggc gatcaccgtc gctcgatgct ccgggtcggg attgagcggg ttcgggtaga
     6781 agccgaggcc ggagatcgaa aggcccttgg cctcgacctc tgcacggatg tcccgggcct
     6841 gtgatgccga caggttcgcc acgtcgatgt ggctggtccc cgcgtaccgg cgcgccgcgc
     6901 cgtcgccccg gggccagcag gcgatctcga ggacctcgaa gcccgccgag gcggcccagt
     6961 ccgccacctc ggtgagcggc gtctccggga acggggcggt caggaagccg agcttcatcg
     7021 ctgctcctcg ggctgcggtc gtgacgtcac cgggccacct ccgcccaggt gttcgagcgt
     7081 gcgctctcgg cgatcgcgtc gttgacgagc atctcgtcat ggccgtcggc gaacgtggcg
     7141 taggccggag acgcggaggg acctccggcg ctgacatcgg cgtagatcgc cgcgaaggcg
     7201 gccttgaacg tgtcggcgaa cccctcagcg tgtccgccgg gaaggtacgc ggcagccgcg
     7261 ccggcccgat tcatcagcgc cgggttgcgg aggaggagct cgtttggctc gtcccg
//
LOCUS       DATBSW010000233         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_304245, whole genome shotgun sequence.
ACCESSION   DATBSW010000233 DATBSW010000000
VERSION     DATBSW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304245"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VIF63_06510"
     CDS             <1..639
                     /locus_tag="VIF63_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239068.1"
                     /translation="DEVLEQVGMSARADTNTETYSRGMLQRLGIADALVKDPSVLILD
                     EPTTAIDPLGVVEILDMLRSLVRDRGLAVLLSSHLLAQVQSVCDRVGIFAAGKLIGVG
                     TVPELASQFGEGMTKIEVGFDDAAESGSRIAGRLGAIEGVASVESPARAGDPFVLVVK
                     PDVARSVREHTLNAAAADGLGLNTIREVVPSLDDIYRQAVTQAGLNVRRAAP"
     gene            717..1664
                     /locus_tag="VIF63_06515"
     CDS             717..1664
                     /locus_tag="VIF63_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8239069.1"
                     /translation="MTVASKELADHILSARFYVLLVVLGVAALIPLYFAAEALRSLAE
                     GVSGVPGIFLALFFFSPEGVDLGPLQFTVRAFVAIAAPLLGVAFGFDAVNGERHEGTL
                     PRLLSQPIHRDDVINGKFAGALAAITVGLTILVLVISGFGMLRLGIIPTIEDVLRLIA
                     WLAVTVLYVSLWLAFGLLLSVVIRRATTSALIGFGAWLFAAIPFIGPLLLNILGSLLV
                     RDATTQAEAYGTIQWLQRLLPGQVYIETTSAILSPATTAIGASEQYSPQKAQQLADTL
                     LTIDQSLLLIWPQIVILFALTIGCFALAYARFMRQEIRA"
     gene            complement(1671..3251)
                     /locus_tag="VIF63_06520"
     CDS             complement(1671..3251)
                     /locus_tag="VIF63_06520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY8239070.1"
                     /translation="MADHALAIDVGTQSVRAILFDPAGNLVAMGRVPIEPYVSPQPGW
                     AEQDPEVWWTAIGDACRKLWAAAGAAGAGGTAIGPDRVAGVALTTQRTTLVVADADNR
                     PLRPAIVWLDQRRTEGLKPLGGFWGAMFRVAGVRETVARFQADAEANWIERNEPAVWK
                     RIRRYGVLSSWLTARLTGDWVDSTASQVGYLPFDFKRSQWVSGFDWKWQVAPFEKAWL
                     PRLVPPTGRLGDLTADAAAHLGVAAGTPLIAAAGDKQCEALGAGALQPEVAALSFGTT
                     ATIGTTHRRYIEAIPLVPPYPAAIPGAWFVELQVLRGFWMVEWFKREFGAAEINRAAG
                     RDIPPEALFDELLSASPPGSQGLLLQPYWMPGIRYPGPEAKGALIGFGDVHGRAHVYR
                     AILEGLAYALREGAERTVKRSKVPIRELRISGGGSQSRAAVQLFADVFGLPASRPHTH
                     EAAGLGAAIDVMLGLGIHPDPIDAVAAMVHVLDRFEPNPAANRTYEDLYRSVYLPMYD
                     RLKPLYREIRRITGYPPT"
BASE COUNT          494 a         1198 c         1203 g          567 t
ORIGIN      
        1 gacgaggtgc tcgagcaggt cggcatgtcg gcccgggccg acacgaacac cgaaacctac
       61 tcacggggca tgctccagcg cctcggcatc gccgatgcgc tggtgaagga tccatcggtg
      121 ctcatcctcg acgagccgac cacggcgatc gacccgctcg gcgtcgtcga gatcctggac
      181 atgcttcgtt cacttgttcg cgaccgaggg ctggcggtcc tcctgtcgag ccacctgctc
      241 gcacaggtgc agtcggtctg cgaccgggtc ggcatcttcg cggcgggcaa gctgatcggt
      301 gtcggcacgg tgccggagct tgccagccag ttcggcgagg gcatgacgaa gatcgaggtc
      361 ggcttcgacg atgcggccga atcgggcagc aggatcgccg ggcgactggg ggcgatcgag
      421 ggcgtggcat cggtcgaatc accggcccgt gcaggcgatc cgttcgtgct cgtggtcaag
      481 ccggatgtcg ctcgctccgt ccgcgagcac acgctcaatg cggccgccgc cgatgggctc
      541 ggcctcaaca cgatccgcga ggtcgtcccg tcgctggacg acatctatcg ccaggccgtc
      601 acgcaggccg gcctcaacgt ccggagggcc gccccgtgag cgccagcgcc gcagccgcac
      661 cagaggcacg ggaacgcacg aggccgatcg ccgaacggcg gctgcgaccc ggttggatga
      721 ccgtcgcgag caaggagctg gctgaccaca tcctcagcgc ccggttctac gtgcttctcg
      781 tggtcctcgg cgtggcggcg ctcatcccgc tctactttgc cgccgaggcg ctccgcagcc
      841 tggctgaagg cgtgagcggg gtgcctggaa tcttccttgc cctgttcttt ttctcgcccg
      901 agggcgtcga tctcggtccg ctccagttca ccgtccgcgc cttcgtcgcc attgccgcgc
      961 cccttctggg cgtcgcattc ggcttcgatg cggtcaacgg cgagcggcac gagggcaccc
     1021 tgccgaggct tctctcccag cccatccacc gtgacgacgt catcaacgga aagttcgcgg
     1081 gtgcgctggc tgcaatcacg gttggcctga cgattctcgt gctggtcatc agcggtttcg
     1141 ggatgctccg cctcggcatc atcccgacca tcgaggacgt gctgcgcctg atcgcatggc
     1201 tggcggtcac ggtgctgtac gtgtcgctgt ggcttgcctt tggcctcctc ctctcggtcg
     1261 tcatccgccg cgccaccacc tcggcgctga ttgggttcgg cgcctggctt ttcgcggcca
     1321 tcccgttcat tggtccgctg ctgctcaata tcctcgggtc gctgcttgtc cgcgacgcga
     1381 cgacacaggc cgaggcctac ggcacgatcc agtggctgca gcggctcctg ccggggcagg
     1441 tgtatatcga gacgacctct gcgatcttga gcccggctac cacggcgatc ggcgcgagcg
     1501 agcagtacag cccgcagaag gcgcagcagc tcgcggacac gctgctcacg atcgaccaga
     1561 gcctgctcct gatctggccg cagatcgtga tcctcttcgc cctgaccatc ggctgctttg
     1621 cgcttgccta cgcccgcttc atgcgccagg aaatccgcgc ctagccccga tcaggtgggc
     1681 gggtagccgg tgatcctcct gatttctcgg taaagcggct tgaggcggtc gtacatcggg
     1741 agatagaccg agcggtagag gtcttcgtag gttcggttgg cggcggggtt cggttcgaag
     1801 cggtccagga cgtggaccat cgccgcgacg gcgtcgatcg ggtcggggtg gatgccgagt
     1861 cccagcatca cgtcgatcgc cgcgcccagg cccgcggctt catgggtgtg cgggcgtgag
     1921 gcaggcaggc cgaacacgtc ggcaaagagc tggaccgcag cgcgggattg cgagccgccg
     1981 cccgagatgc gcagctcgcg gatcgggacc ttgctgcgct tgaccgtgcg ttcggcgccc
     2041 tcgcgcaggg cataggcgag gccttcgagg atcgcccggt agacgtgggc ccggccatgg
     2101 acgtcgccga atccgatcaa cgcccctttc gcctccggcc cggggtagcg gatgcccggc
     2161 atccagtacg gctgcaggag caggccctgc gacccgggtg gcgaggccga gaggagctca
     2221 tcgaacaggg cctcgggcgg gatgtcacgg ccggcggcgc ggttgatctc ggccgcgccg
     2281 aactcgcgct tgaaccactc gaccatccag aagccgcgca gcacctgcag ctcgacgaac
     2341 caggcgcccg ggatggccgc cgggtacggc ggcacgaggg gaatggcctc gatgtagcgc
     2401 cggtgggtgg tgccgatggt cgccgtcgtg ccgaaggaga gggccgcaac ttcgggctgg
     2461 agcgccccgg cgccgagcgc ctcgcactgc ttgtcgccgg cggctgcgat cagtggcgtt
     2521 ccggccgcga ccccaaggtg cgccgcggca tcggccgtga ggtcgcccag gcggccggtc
     2581 ggtgggacga ggcgcggcag ccaggccttc tcgaacggtg cgacctgcca cttccagtcg
     2641 aacccgctca cccattggct gcgcttgaag tcgaacggga ggtagccgac ctggctcgcg
     2701 gtcgagtcga cccagtcgcc ggtcagccgg gccgtcagcc acgacgacag cacgccgtac
     2761 cggcgaattc gcttccacac ggccggctcg ttgcgctcga tccagttcgc ttcggcgtcg
     2821 gcctggaagc gggcgaccgt ctcgcgcacc ccggccacgc ggaacatggc gccccagaag
     2881 ccgccgaggg gcttgaggcc ctccgtgcga cgctggtcca gccagacgat cgccggtcga
     2941 agcggacgat tgtccgcatc ggcgacgacg agcgtcgtgc gctgcgtcgt cagtgcgacg
     3001 ccggcgaccc ggtccggacc gatcgccgtc ccacccgctc ccgcggcacc ggctgctgcc
     3061 cacagcttcc ggcaggcgtc gccgatggcc gtccaccaga cctcgggatc ctgctcggcc
     3121 cagcccggct gcggcgagac gtacggctcg atcggcactc tgcccatggc cacgaggttg
     3181 cccgcggggt cgaagaggat cgcccggacg ctctgcgtcc ctacatcgat cgccagcgcg
     3241 tgatccgcca cgcggtgcat ggtggcggat cgggtgcccg tcctgtcaga cgggcggcca
     3301 ggggatggcg ggccagtcgg ggctggggct cgggaagttg ccgctcgcga ggagtgcggc
     3361 gactctcgcc cgcgtcgcct cgatctcgtc gatgaacagc aggtccgcga gcgcaacccc
     3421 gagtggaccc ggtgtcgcga cggtcccgag cgcgtcgcgc ac
//
LOCUS       DATBSW010000234         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_198652, whole genome shotgun sequence.
ACCESSION   DATBSW010000234 DATBSW010000000
VERSION     DATBSW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198652"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VIF63_06525"
     CDS             complement(<1..929)
                     /locus_tag="VIF63_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8239071.1"
                     /translation="MRVVVVGAGVMGAWTARWLRRGGHEVTLVDRHGPGNQLGSSGDV
                     SRVTRSAHGPDRFYPTWQRRALDQWRDLERSSGTSLFIQSGVVWLANEAQTFETESVE
                     SLEALAIPFERWSHDDFAARVPVMSADGVPWALFEPEAGALLARPGVVATIEAFEADG
                     GEVVVGRVAPPDGSRAGGGAADGALDRVRLDDGRTLEADAFVFACGPWLPTLFPALLG
                     ELIVTHRQDVMQFAVPAGDARFGPDTLPVWIDFEGSFYGFPSLDGVALKACPDWLGPI
                     EGPDESARVVAEATVEASRTVLRRRFPAIADQRV"
     gene            1145..2275
                     /locus_tag="VIF63_06530"
     CDS             1145..2275
                     /locus_tag="VIF63_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8239072.1"
                     /translation="MNHTTSLPPPDARRRQARRLPLVIAIVAGALIAASAPAVAYGWD
                     NYSFSSASEDQMITLINQARASAGKAPLAELSSLSSVARWRSKDMWDRDYFCHSIPPS
                     GASIPDLCDDFPSSWPKVFDELQDRGICYTLAGENIAVNNYPDDTATQVAFNGWMNSQ
                     THRDLILGSGFTKIGMGAFKGTGTQYPKHFWTAVFTHPCAGSTPTPTPTPKPTPKPTP
                     KPTPKPTATPHPTPHPTATPTDTPDPTPHPTPRPTPKPTAEPTDEPIPDQSHEPTPEP
                     SPEPTVEVTPEPDITPGPVDALIGGAFVAHWVDALSEDEFATEPPPTPTASDEPPESL
                     PPDGGPLVTDGDGGSLQVLEPPPSMGLVDTIVGDVVASFLGK"
     gene            2338..3147
                     /locus_tag="VIF63_06535"
     CDS             2338..3147
                     /locus_tag="VIF63_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239073.1"
                     /translation="MTTRRTWSELRPANPQPTSGVQMPPILEARDLTKVYALGKTSVE
                     ALRGVTMSVAPGEFVALMGPSGSGKSTLLQLLGGLDRPTTGSVIFEGMDISDLSDDKQ
                     TRLRRERTGYIFQAFNLIPLLNVRENIALPFTIAGGQKDQKERVRQVIDLVELTGKER
                     HRPDQLSAGEQQRVAVARALVTRPSVLLADEPTGNLDYTNSGELLDALWRSCDQGGQT
                     IVLVTHDARAAAYADRVLVIGDGHIQEEIVLGRRADHSAASLIERLAQVGL"
     gene            3152..>3767
                     /locus_tag="VIF63_06540"
     CDS             3152..>3767
                     /locus_tag="VIF63_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8239074.1"
                     /translation="MGLDRLAWRAVIARPLRSFLTILGVALGIAVLSASLTLGAALDQ
                     AVDRTVRDMVGRADLRVSGFVERGLSEQAVDTIVGSQGVLDAAPVIEHRTFPLGTPTG
                     ATADAVTVLGVDPASYLRLHDLPIVAGSGLAGASEPVVLITEELAAQDGYVVGGTLSL
                     LGAGGVTDLRIVGIVPGFGPLPGPGRTVIIPIDVARSTFGLEGAT"
BASE COUNT          575 a         1426 c         1245 g          521 t
ORIGIN      
        1 acgcgctggt cggcgatcgc ggggaagcgc cgccggagga ccgtccgcga cgcctcgacc
       61 gtcgcctctg ccacgacgcg cgctgattcg tccggcccct cgatcgggcc gagccagtcg
      121 gggcaggcct tcagcgcgac gccgtcgagc gagggaaagc cgtagaacga gccctcgaag
      181 tcgatccaga ccggcagcgt gtccgggccg aagcgcgcgt cgcctgcggg aaccgcgaac
      241 tgcatcacgt cctggcggtg ggtcacgatc agctcgccga gcagcgccgg aaagagcgtc
      301 ggcagccacg gcccgcaggc gaagacgaac gcgtccgcct cgagcgtccg cccgtcgtcg
      361 agccggaccc gatcgagcgc gccgtccgct gcgccgccgc cggcgcgtga cccgtcgggc
      421 ggcgcgacgc gacccacgac cacctcgccg ccgtcggcct cgaaggcctc gatcgtcgcg
      481 acgacgccgg gtcgtgcgag cagcgcgccc gcctcgggct cgaacagcgc ccagggcacg
      541 ccgtcggcgc tcatgacggg tacccgcgcc gcgaagtcgt cgtgcgacca gcgctcgaat
      601 ggaatggcga gggcctcgag cgactcgacg gattcggtct cgaacgtctg cgcctcgttg
      661 gccagccaca cgacgcccga ctggatgaag agcgacgttc ccgaggagcg ctcgaggtcg
      721 cgccattggt cgagcgcccg gcgctgccag gtcgggtaga agcggtccgg cccgtgggcg
      781 cttcgcgtca cccgcgacac gtcgcccgat gacccgagct ggttgcccgg cccatgccgg
      841 tcgacgagcg tgacctcatg cccgccgcgg cgcagccagc gggccgtcca cgctcccatg
      901 accccggcgc cgacgacgac aacccgcatg cgcggatcgt actggctcgt gctggtggtg
      961 cgtccgggca ccactcggtg gggtctggtg gggtttggca tagacgcgtt ggtacccatg
     1021 gtccgatcgg tcgggacctt tccggcaggg ccgaagtgcc ctcttgtcgt ccagagtgcg
     1081 gttggttcgg tttgcgagct gccaggggca gcaaggcccg gaccgtgccc cgccttggag
     1141 tctgatgaac cacaccacct cgctgccccc gcccgatgcg cgacggcggc aagcccgccg
     1201 cctgccgctc gtcatcgcga tcgtcgccgg ggcgctgatc gccgcgagtg cgccggccgt
     1261 tgcctacggc tgggacaact actcgttctc ctcggcgagc gaagaccaga tgatcacgct
     1321 gatcaaccag gctcgcgcct ccgccggcaa ggcgccgctg gccgagctct cgagcctctc
     1381 gagcgtggcc cggtggcggt cgaaggacat gtgggatcgc gactacttct gccacagcat
     1441 cccgccatcc ggggcgagca ttcccgatct gtgcgatgac tttccgtcga gctggccgaa
     1501 ggtgttcgac gagctccagg atcggggcat ctgctacacg ctcgccggcg agaacatcgc
     1561 cgtgaacaac taccccgacg acaccgccac gcaggtggcg ttcaacggct ggatgaactc
     1621 ccaaacgcac cgtgatctca tcctcggctc cggcttcacg aagatcggca tgggcgcctt
     1681 caagggcacg ggcacccagt atccgaagca cttctggacg gctgtgttca cccacccgtg
     1741 cgcgggctcg acgccgacgc cgacgccgac cccgaagccg acgcccaagc caacgccgaa
     1801 gccaaccccg aaaccgaccg caacgccgca cccgaccccg cacccgaccg cgacgccgac
     1861 cgacacgccc gacccgacgc cgcaccccac gcccaggcca accccgaagc caacggccga
     1921 accgaccgac gaaccgatcc ccgaccagag ccacgagccg acgccggagc cgtcgccaga
     1981 gccgaccgtc gaggtcacgc ctgaaccgga catcaccccg ggcccggtcg acgccctcat
     2041 cggcggcgcg ttcgtcgcgc attgggtcga tgcgctgtcg gaggacgagt tcgcgaccga
     2101 gccgccgccg acgccaactg ccagtgacga acccccggag tccctgccgc cggacggcgg
     2161 accgctcgtc accgacggtg acggtggatc gctccaggtg ctcgagccgc cgccctcgat
     2221 gggcctggtc gacaccatcg tcggcgacgt cgtcgcctca ttcctgggga agtgatgacg
     2281 agcctccctc atcgcgccat gccaaccttg tttgccgcca cactggagcg ggagcgagtg
     2341 accacgcgac ggacgtggtc cgagctccga cccgccaacc cgcaaccgac cagtggagtc
     2401 cagatgccgc cgatcctcga ggcgcgcgac ctgacgaagg tctatgcgct cggcaagacg
     2461 agcgtcgaag ccctccgcgg cgtgacgatg tccgtcgcgc ccggcgagtt cgtcgcgttg
     2521 atgggtccat ccggctccgg caagagcacg ctgctccagc tcctgggcgg cctcgaccgg
     2581 ccgacgaccg gctcggtcat cttcgagggc atggacatca gcgacctgtc cgacgacaaa
     2641 cagacgcgcc tgcgccgcga acggaccggc tacatcttcc aggcgttcaa cctgatcccg
     2701 ctgctgaacg tgcgcgagaa catcgccctg ccgttcacga tcgcgggcgg ccagaaggac
     2761 cagaaagagc gtgtccgcca ggttatcgac ctcgtcgagc tgaccggcaa ggagcgccac
     2821 cgcccggacc agctgtcggc cggcgagcag cagcgcgtcg ccgtggcgcg cgccctcgtg
     2881 acgcggccgt cggttctgct cgccgacgag ccgaccggca acctcgacta cacgaacagc
     2941 ggcgagctcc tcgacgcgct gtggcggtcc tgcgaccagg gcggccagac gatcgtcctc
     3001 gtcacccacg acgcgcgcgc cgcggcgtac gcggaccgcg tcctcgtcat cggcgacggt
     3061 cacatccagg aggagatcgt gctcggccgc cgggcggacc actccgccgc ctcgctgatc
     3121 gagcgcctgg cccaggtcgg cctctaggcc ggtgggtctc gaccggctgg cgtggcgggc
     3181 ggtcatcgcg cgaccgctgc gctccttcct cacgatcctc ggcgtcgccc tcggtatcgc
     3241 cgtcctgtcg gcctcgctga cgctgggcgc ggccctcgac caggcggtcg accggaccgt
     3301 tcgggacatg gtcggccggg ccgacctgcg cgtctctggc ttcgtcgaga ggggcctctc
     3361 cgagcaagcc gtcgacacga tcgtcggcag ccagggcgtc ctcgatgccg cccctgtcat
     3421 cgaacatcgg acattcccgc tcgggacccc cacaggcgcc accgccgacg cggtgaccgt
     3481 gctgggcgtc gatccggcgt cctatctccg cctgcacgac ctgccgatcg ttgccggatc
     3541 cggccttgcc ggagcatccg agccggtcgt gctcatcacc gaagagctcg cggcccagga
     3601 tggctacgtc gtcgggggca cgctttcgct cctcggcgcg ggcggcgtca cggacctccg
     3661 gatcgtcggc atcgtccccg gcttcgggcc gctcccgggc cccggccgga ccgtgatcat
     3721 cccgatcgac gtcgcccgct cgacgttcgg cctcgagggc gcgactc
//
LOCUS       DATBSW010000235         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_868360, whole genome shotgun sequence.
ACCESSION   DATBSW010000235 DATBSW010000000
VERSION     DATBSW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868360"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VIF63_06545"
     CDS             complement(<1..2053)
                     /locus_tag="VIF63_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY8239075.1"
                     /translation="MPRPVAAAAGDGAEGATDVAPDVAQVPSIHYEQALEHEGLKLTF
                     EPGDRVTVPFSPRAGDDWAVDGKAARELPAGHATGVKMRDARASSIWAAGPPNDVVGR
                     DHAGGPGGPRESLSGVDVDGGGPTVLADPASAATGSTADDTVDAAPIGANGLRREVFG
                     FLPYWELTDSTTTLDWRTLSTVAYFSVGCTSSGGLEKVDPDGTLTAAWGGWTSSKMTS
                     IINAAHKNQTRVVLTISCFAWTGSGASRQASVLGSARARSNLAKAAAAAVRDRGADGI
                     NLDFEPIVAGYADEFTALVRSVRSELNKIAPGYQLTFDTLGAIGNQPIVNATAPGGAD
                     AVLIMGYDYRTASSADAGSVSPLSGPRYDLTDTVKAYTAKISPSKVILGVPWYGRAWS
                     TASDRPHASTLSSAKYGSVASPTYGQVMDLVVQHGRRYDSVEQAPWTAYRKQTCTSTY
                     GCVTSWRELYYDDAASLRLRYDLVNRTQLRGVGIWALGYDGTRPELRAALADKFLSDK
                     TAPIAGVATLAQTQRDEGFKVRWTSWDDSAIIAYDVQVSVDGGAWTPWLTGTTLTNSI
                     FLGEDGRTYAFRVRAADAHGNVTAWLAAASAASTAIPDKISVGGFATVLTDGLRMRSA
                     PSTDATIMVTLADGDALQVIGGPVTAEGYTWFQVAGPVQQWGPVDPVQVGGWVAASGN
                     GA"
     gene            complement(2309..4480)
                     /locus_tag="VIF63_06550"
     CDS             complement(2309..4480)
                     /locus_tag="VIF63_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239076.1"
                     /translation="MRFRAPLAAAIAALVALAPVMALPALASSSAPAGAGSLAAGYVS
                     NAKIVIVVGAVEGSTPGYRSKGDQIYAEAIKYTPNVTKIYSPNATWAKVKAAAQGANI
                     FIYAGHGYGFPSPYRPILSPSVQDGMGLNEVGGINDSDKKYYGESSIASEIRFAKNAV
                     VLLNGLCYSAGSSEDGDPEPTMAVARERVDNFASGFIKAGARVVIAQSWTSGVVYALR
                     TIFTSDQSFEQLWENAPNAQGNDQPFIPARNPQFQGRVDPDEPNSGFHRSFVGAPDML
                     TSDVLAGVGVAQTASTPDTTSPELWSLDGARALTPNFDGDADKLNLLARFSETVGWTA
                     EIRNADGDVVRTQTGSGHQAAVTWDARVAGELAPDGDYTWNLHATDAAGNPPLDESGP
                     LTVENQASPDTGVLSFNATTPLMTTSGSITFALTFAAPVTGLAKADFTRVGTAPSCVF
                     GQPVGSGAAYTIAATGCSTGTVGLYLNAGTVQDASLNVGPAGPISTARVTIDRTAPKA
                     TTPKPTLRTDLALETASASQRLLMKLTWTGTDTGSGIASYDVQRAYDGAAYQTIASAT
                     PATTLSWTMTPGHAYKFRVRARDKAGNVGSWTTAYTWHASLIQNSSSSLAGTGSWSTS
                     SAAAHSGGSVKSSTAAGASVSYTFSGRAIGWVTTLGVFGGPVQVWIDGALAATVDTHA
                     DVTTYRQVVFTKGWSSYGSHTIKLVVVAGGKAVLDAFEVIR"
BASE COUNT          635 a         1586 c         1757 g          742 t
ORIGIN      
        1 tcgcgccgtt gccggaggcc gccacccagc caccgacctg gaccggatcg accggccccc
       61 actgctgcac ggggccggcg acctggaacc acgtgtagcc ctcggccgtg accgggccgc
      121 cgatcacctg cagcgcgtca ccatcggcga gcgtgaccat gatcgtggcg tccgtgctcg
      181 gtgccgagcg catccgcagg ccgtcggtca gcaccgtcgc gaacccgccg accgagatct
      241 tgtcggggat ggcagtcgag gccgcggagg ccgccgcgag ccacgcggtc acgttgccgt
      301 gggcatccgc cgcccggaca cggaaggcgt aggttcgccc gtcctcgccg aggaagatgc
      361 tgttcgtgag cgtcgtgccc gtgagccacg gcgtccaggc gccaccgtcg accgagacct
      421 ggacgtcgta ggcgatgatc gcgctgtcgt cccacgaggt ccacctgacc ttgaagccct
      481 cgtcgcgctg ggtctgggcg agcgttgcca cacccgcgat cggcgccgtc ttgtcggaga
      541 ggaacttgtc ggccagtgcg gcgcgcagct ccggccgcgt gccgtcatag ccgagcgccc
      601 agatgcccac accgcggagc tgggtccggt tgacgaggtc gtagcgcagg cgcagcgacg
      661 ccgcgtcgtc gtagtacagc tcgcgccagg acgtgacgca gccatacgtg ctcgtgcagg
      721 tctgcttgcg gtaggcggtc cagggcgcct gctcgaccga gtcgtagcgc cggccgtgct
      781 ggacgacgag gtccatcacc tggccgtagg tcggggacgc gacgctgccg tacttggcgc
      841 tcgagagcgt gctcgcgtgc gggcggtccg atgccgtcga ccacgcccgg ccgtaccacg
      901 gcacgccgag aatgaccttc gacggcgaga tctttgcggt gtacgccttg acggtatcgg
      961 tcaggtcgta gcgcggcccg gacaggggcg agaccgagcc ggcgtccgcg gagctggcgg
     1021 ttcgatagtc gtagcccatg atcagcacgg cgtcggcgcc gcccggcgcc gtcgcgttca
     1081 cgatcggctg gttgccgatc gccccgagtg tgtcgaacgt gagctggtag cccggcgcga
     1141 tcttgttgag ctcgctgcgc accgaccgga ccagcgcggt gaactcgtcc gcgtagcccg
     1201 cgacgatcgg ctcgaagtcg aggttgatgc cgtcggcgcc gcggtcgcgg acggccgcgg
     1261 cggcggcctt cgccaggttc gaccgggccc tcgcgctgcc gagcacgctc gcctgcctgc
     1321 tcgcgccgga gcccgtccag gcgaagcacg agatcgtgag gacgacgcgc gtctggttct
     1381 tgtgggcagc gttgatgatc gaggtcatct tcgagctggt ccagccaccc caggcggcgg
     1441 tcagggtgcc gtcgggatcg accttctcca ggccgcccga tgacgtgcag ccgacgctga
     1501 agtaggcgac ggtcgagagc gtccgccagt cgagggtcgt ggtcgagtcg gtcagctccc
     1561 agtacggcag gaagccgaag acctcgcggc ggaggccgtt cgccccgatg ggcgcggcat
     1621 cgaccgtgtc gtcggcggtg gaacccgtcg ccgcggaagc cgggtcggcc aagaccgtgg
     1681 ggccgccccc atcgacgtcc acgccggaga gggattcgcg aggcccgccg gggccgccgg
     1741 cgtgatcgcg gccgacgacg tcattcggcg gtccggccgc ccagatcgag ctcgcgcgag
     1801 cgtcgcgcat cttgacgccc gtggcatggc cggcggggag ctcccgcgct gccttgccat
     1861 cgaccgccca gtcgtcgccg gcgcgagggc tgaacggcac ggtcacccga tcgccgggct
     1921 cgaacgtgag cttgaggccc tcgtgctcga gggcctgctc gtagtggatc gacgggacct
     1981 gagcgacgtc cggggcgacg tccgtggcgc cctcggcccc gtccccggcg gcggccgcga
     2041 cgggtcgcgg catggcaccc agcggcgcgc acagcacgcc tgcaaaggcg agtgcgagcc
     2101 agggactcga cgtgcggcga gatcgcgagc tgcgctgggt caccggctcc accgtgcgct
     2161 cggctgtcct ggtcggttcg cccgcgtcgg gcatggtttg ccgaggacgg tcgacggtag
     2221 gttgcccgtg ctgccggtca tagccctacg aaagttcgac gaagggtggt accggggggt
     2281 ccggtggggt ctgctgtaga cccagccctc agcggatgac ctcgaaggca tcgagcacgg
     2341 ccttgccccc ggcgacgacc acgagcttga tcgtgtggct gccgtagctc gaccagccct
     2401 tggtgaagac gacctggcgg taggtcgtga cgtctgcgtg ggtgtcaacc gtcgcggcga
     2461 gcgcgccgtc gatccagacc tggaccggcc cgccgaacac gccgagcgtg gtgacccagc
     2521 cgatggcgcg gccgctgaac gtatagctga cgcttgcccc ggccgcggtc gaggacttga
     2581 cggacccacc gctgtgcgcg gccgcggagc tggtcgacca gctgcctgtc ccagcgagcg
     2641 acgagctcga gttctggatc aggctggcgt gccatgtgta cgccgtcgtc catgagccga
     2701 cgttgcctgc cttgtcccgg gcccggacgc ggaacttgta cgcgtggccg ggggtcatcg
     2761 tccagctcag cgtcgtcgcc ggcgtggcgc tcgcgatcgt ctggtacgcc gcgccgtcgt
     2821 aggcgcgctg gacgtcgtag ctcgcgatgc cggaaccggt gtccgtgccc gtccaggtga
     2881 gcttcatgag cagccgctgg ctcgccgagg cggtctcgag cgccaggtcc gtgcgcaggg
     2941 tcggcttggg cgtcgtcgcc ttcggcgcgg tcctgtcgat cgtgaccctg gcggtgctga
     3001 tcgggccggc cggcccgacg ttgagcgacg cgtcctggac cgtgccggca ttcaggtaca
     3061 gccccaccgt gccggtcgag cagccggtgg ccgcgatcgt gtacgcggcg cccgagccca
     3121 cgggctgtcc gaagacgcag ctcggtgcgg tgccgacgcg cgtgaagtcg gccttggcca
     3181 gccccgtcac cggcgccgcg aacgtcagcg cgaaggtgat cgagccggag gtcgtcatga
     3241 gcggcgtcgt cgcgttgaag gacaggacgc cggtgtccgg gctcgcctgg ttctcgacgg
     3301 tgagcggccc gctctcgtcg agcggcggat tgccggcagc gtcggtggcg tgcaggttcc
     3361 aggtgtagtc accgtccggc gccaactcgc ccgccactcg ggcgtcccac gtgaccgccg
     3421 cctggtggcc ggacccggtc tgggtgcgca cgacgtcgcc atcggcgttc ctgatctcgg
     3481 cggtccagcc cacggtctcc gagaaccggg cgagcaggtt cagcttgtcg gcgtcgccgt
     3541 cgaagttcgg cgtgagcgcc cgcgcgccgt cgagggacca cagctccggg ctcgtcgtgt
     3601 cgggcgtgct cgcggtctgc gcgacgccga ccccggcgag gacgtcgctc gtcagcatgt
     3661 ccggcgcgcc cacgaaggag cgatggaagc cgctgttcgg ctcgtcggga tcgacccggc
     3721 cctggaactg cgggttgcgc gcgggaatga acggctggtc gttgccctgg gcgttcgggg
     3781 cgttctccca gagctgctcg aacgactggt ccgaggtgaa gatcgtcctg agggcataga
     3841 cgacgccgct cgtccacgac tgggcgatga cgacgcgcgc gccggccttg atgaagccgg
     3901 atgcgaagtt gtcgacgcgc tctcgagcaa cggccatggt gggctcggga tcgccgtcct
     3961 cggaggagcc ggcggagtag cagagcccgt tcagcagcac caccgcgttc ttggcgaagc
     4021 ggatctcgct ggcgatgctc gactcgccgt aatacttctt gtccgagtcg ttgatcccgc
     4081 cgacttcatt gaggcccatg ccgtcctgga cgctggggct caggatcggt cggtacgggc
     4141 tcgggaagcc gtagccgtga cccgcgtaga tgaagatgtt cgcgccctgt gccgcggcct
     4201 tgaccttcgc ccacgttgcg ttcgggctgt agatcttcgt gacgttcggg gtgtacttga
     4261 tcgcctcggc gtagatctgg tcgcccttgg agcggtaccc gggcgtgctg ccttcgacgg
     4321 cgcccacgac gatgacgatc ttggcgttgc tgacgtagcc ggcggccagg gacccggccc
     4381 cggcgggcgc cgacgaggag gcaagtgccg gcagggccat aacgggggcg agcgcgacga
     4441 gggcggcaat ggccgccgca agcggggcac ggaagcgcat gcgggaggaa ctccggtgac
     4501 gttgagccag tgcgagatga gtgcccgtcc agcctcccgg tggggcgggc atggctcaat
     4561 ggccaatgag tccgggggta ctgcgtggag gtgggtgggg tccgttgccg ggtggtgcag
     4621 tgcgggccgg ttcccgcgaa cggcggggcc gcacgtgttg ccccgaagcc ccgcaaagat
     4681 cgcgctacgg ccggggtgag gggctcccgg tggggccatc
//
LOCUS       DATBSW010000236         6755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_833452, whole genome shotgun sequence.
ACCESSION   DATBSW010000236 DATBSW010000000
VERSION     DATBSW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833452"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VIF63_06555"
     CDS             <1..1152
                     /locus_tag="VIF63_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239077.1"
                     /translation="ASKDVSVSGDATVAVVRSAMTCPALEHMIGASAWSIAGRQTQQN
                     SSPVGDLNLTVLTLTNGDETALAYLAGGENCTARIARTTPALLRVSGAATFDGSADLD
                     VPLCIASGKTVTFAAILRAPGGPNVQVTVVAPVRTGTARLPGDDVTVGVTRGAMSTTK
                     LAGVLIKAFTDFDGARFVEAYEPTDDSRVRLDVETIDPFVATLHVTDFEGEAGTISLD
                     AKVACHLPGGSLRRAAELAAVPAATTPPGTPGPTLPPFDGAAPGTAVVTGSQIAGTYQ
                     MPASGLDCYYDFDQGAWTILSVGGADPTFYLYAFAGQEGGSTSQVSMGLIVGDGGNGK
                     LLLFDTHVSPPDGTAQATVAQSGGTVSVVVQGRTSAGATLSANLSCAGS"
     gene            1176..1487
                     /locus_tag="VIF63_06560"
     CDS             1176..1487
                     /locus_tag="VIF63_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239078.1"
                     /translation="MFLLIEGPLLVVAPQLSAAVAEARAGGWTIVRGWGAPLTRERAI
                     CVGWIRTVDDARRALLAAVAGAGLILGVSTDTETVDRLLDDLRRLGVVEHVRSQEHGP
                     H"
     gene            1510..2010
                     /locus_tag="VIF63_06565"
     CDS             1510..2010
                     /locus_tag="VIF63_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239079.1"
                     /translation="MVAPIVVVEGPEEAFTRVASELRAAGWNVTDGFERQVPRVDRAV
                     RSGTVAGPRDAAAALLAALDGVGLVIHGCAPRSVLDRLLDDLRHVGPVEHRRRLEPAP
                     PALDADELAILRMLGEGQRLGDAADALGLSRRTADRRLAAARRALGTERTVEAVSRAR
                     RLGWLR"
     gene            2063..4576
                     /locus_tag="VIF63_06570"
     CDS             2063..4576
                     /locus_tag="VIF63_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8239080.1"
                     /translation="MEGPGSDLIGRDVELRALVAAIEADQGAIVVGEAGIGKTALVRA
                     AVATTGRRLHEGGGFATLNRLPYLALRRATGAGLAGDAAAVAAQVERLVGPDVLFVDD
                     LHWVDVDSQAVIALLADRILIVAAIRTGDPGAAAALELVQRSGSVLITLDGLGDAAAA
                     RLIQRARPEASERVAAGIVRQAGGNPLLLEELALGGEASTALERALVSRFERLDRDAQ
                     ESLELLAVADRALPRDAAGPGAAAALEAGLARQTPDGMAIRHQLVADAIQEQLTLDRR
                     AALHVQLAALATDPVEQARHLVAGGRAAEAEAMALEAIGSTIDARRRATLLEVAAEAG
                     GAGSRLDLRLPAARALDELSDWPGVRRVLGVPAEGSAEELCERDVIIAHATFAMGDPV
                     GARRSLDDAAERGAAPQSEAAARLAIEEATYLVNVEGRVAEAIARLAGVLSGNPAPET
                     VRDASVLRACILLLATGEGDPGVIGAGLEEAFAAGRYRTATDRARVLQYAMLMGVSTE
                     EALGFLLAQHRRFDAAGVGVAALEFLADAVVAAVLAGHLEQALLLADELLEQPAPPRA
                     VQTAAIHRAQALTLAGRFDQAESTLAEALRVASPDWFGRGLGLAVQAELAYWSGRYDA
                     AETLALAALGVPAPVPVAHVNVLLTQAWVRTELGHDPGGPLAVDLTPSLAGARNEVDG
                     LRAWHAGDMAAARTAFVEAGRQWAAFHVPHYLVCRWAAAESLRRSGDVEAAAAELADV
                     LAEATRAGMEPVAARIRRSLRLAGVRSSAEPRGHPSDSLGLTPRERELVALVEQGLTN
                     VEIARRLGLGRPTVTRILGSAMTKLGVGSRAQMVARLGS"
     gene            complement(4586..5149)
                     /locus_tag="VIF63_06575"
     CDS             complement(4586..5149)
                     /locus_tag="VIF63_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239081.1"
                     /translation="MRFPRTVVSFAITGLLVAACGGAAPPTAGPDGVTPTANPGGGQG
                     TPTQAPVATPDGGGGGVLPGNGSGKVNFEISGPVQKSGELPFFGAGSRFSGPAGVSLQ
                     FTRTDSSATEIVSIFPDLSDSTKWLVSYVSDDGLVSATACTMSNLQLSDTNASGAFDC
                     PNSIVTKADGTYATDGRIRGTFEAHAT"
     gene            complement(5217..5804)
                     /locus_tag="VIF63_06580"
     CDS             complement(5217..5804)
                     /locus_tag="VIF63_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239082.1"
                     /translation="MPAEADAEPIPSIRTPRLELVSMSIPFMHALVADDLEAASREIG
                     AAVPDGMSEDLVDFLRYRLTQLAVDPSILEWLGRAMVLNDETGARCVIGTIGFHGAPD
                     AQGRVEVGYRVEPGFRRQGYAREAVRAMFDWAATTHGIRRFIASVSPTNAASLGLIAE
                     FGFSQTGSQMDEIDGLELVFEADWPPPAGLQVGVD"
     gene            complement(5794..>6755)
                     /locus_tag="VIF63_06585"
     CDS             complement(5794..>6755)
                     /locus_tag="VIF63_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8239083.1"
                     /translation="ENPTAHEATTGPELWEQTEGRITHFVASVGTGGTITGAARYLRT
                     QKADIVVVGADPEGSVLSGDVARPYLTEGVGEDFFPGTYDAAMVDRWVRVSDRDAFAM
                     ARRITREEGILAGESCGTAVVAALDEARRIMHERGAAASEAVMVVIFPDGGRNYLSKL
                     YNDEWLRANGLLSTTGGVIRIDELLRARHPEPGMPAVVLARTTDRVGAAIDLLQLYGI
                     SQLPVSERPNDDAVEGIVGSVSEKSLLDRAYRDPTVVERTVGEVMDKPLPTIDLGASL
                     DDAFALLSGAAAAVVAVSAGRPAGVVTKLDLLEFLAHRSPRAG"
BASE COUNT          900 a         2429 c         2437 g          989 t
ORIGIN      
        1 gcgagcaagg atgtgagcgt gagcggcgac gcgacggtcg ccgtcgtgcg cagcgcgatg
       61 acctgtcccg ccctggagca catgatcgga gcgagcgcct ggtcgatcgc cggacgccaa
      121 acgcagcaga acagctcgcc ggtcggcgac ctgaacctga ccgtcctcac gctcacgaac
      181 ggtgacgaga ccgcgctcgc gtacctggcg ggcggcgaga actgcaccgc tcgcatcgcc
      241 cggaccacgc cggcgctgtt gcgcgtctcc ggtgcagcga cattcgatgg gagcgccgac
      301 ctggacgtgc cgctctgcat cgcgagcggc aagaccgtca cgttcgccgc catccttcgc
      361 gcgccgggcg gcccgaacgt ccaggtcacc gtggtcgcgc ctgtccggac cgggacagcg
      421 agactgcccg gggatgatgt cacggtcggc gtgacgcgcg gcgcgatgag caccaccaag
      481 ctcgccggcg tcctcatcaa ggccttcacg gacttcgacg gagcccgctt cgtcgaggca
      541 tacgagccca cggacgactc gagggtgcgg ctcgacgtcg agacgatcga cccgttcgtg
      601 gcgacgctgc acgtgaccga cttcgagggc gaggccggca cgatcagcct cgacgccaag
      661 gtcgcctgtc acctgcccgg cggctcgctc cgacgcgccg ccgagctcgc tgccgtaccg
      721 gcggccacga cgccacccgg gacgcccggg cctaccctcc cgccgttcga tggcgccgcg
      781 ccgggcacgg ccgtcgtcac cgggagccag atcgccggca cgtaccagat gcccgcgtcc
      841 gggctcgact gctactacga cttcgaccag ggggcgtgga cgatcctgtc cgtcggcggc
      901 gccgatccga ccttctatct gtacgcgttc gcgggccagg agggtggctc gacgagccag
      961 gtctcgatgg gcctcatcgt tggtgatggc gggaacggca agctcctgct gttcgacacg
     1021 cacgtttcac cgcccgacgg cacggcccag gcgacggtcg cgcaatccgg cgggaccgtg
     1081 agcgtcgttg tccagggccg gacctcggcc ggtgcgaccc tctcggccaa cctgtcgtgc
     1141 gcagggtcgt aacgactccg gcggccgcta cgcaggtgtt cctgctcatc gaggggccgc
     1201 tgctggtcgt tgcgccccag ctctcggccg ccgtggccga agcccgagcc ggcggctgga
     1261 ccatcgttcg tggctggggt gcgccgctca cgcgcgagcg cgccatctgc gtcggctgga
     1321 tccggaccgt cgacgacgcc cggcgggcgc tccttgccgc agttgcgggc gccggcctca
     1381 tcctcggggt gagcacggac acggagaccg ttgatcgact cctcgacgat ctcaggcgcc
     1441 tcggagtcgt ggagcatgtc cgaagccagg agcacggccc ccattgacgg caggccgcac
     1501 gatgggccca tggtggcgcc gatcgtcgtg gtggaagggc cggaggaggc gttcacgcgg
     1561 gtcgcgtccg agcttcgggc ggcaggctgg aatgtgaccg acggtttcga gcggcaggtc
     1621 ccgcgggtgg atcgcgccgt gcgttcgggc accgtcgccg gaccacggga cgccgctgcg
     1681 gccctgctcg cggcgctgga tggcgtcggg ctcgtcatcc acggctgcgc gccccgctcc
     1741 gtgctggatc ggctcctcga cgacctgcgc cacgtcgggc cggtcgagca tcgacgccgc
     1801 ctggagccgg ctccgcctgc gctcgacgcc gacgagctgg cgatcctcag gatgctcggc
     1861 gaggggcagc ggctcggcga cgccgcagac gccctggggc tgtcgcgccg gacggcggat
     1921 cggcgcctcg ccgcggcccg acgggcgctc gggaccgagc ggacggtgga ggccgtgtcg
     1981 cgggcacgac ggctcggctg gttgcgctga cctcccgcgg attctcgttg cgtcggcggc
     2041 tcgggggccc gaccatggtc gagtggaggg accgggctcg gacctgatcg gtcgcgacgt
     2101 cgagctgcgc gcgctggtcg cggcaatcga ggctgatcaa ggtgccatcg tcgtcggcga
     2161 agcgggtatc ggcaagacgg cactcgtgcg ggcggccgtg gcgacgacgg gcaggcggct
     2221 gcacgagggc ggcggattcg cgaccttgaa tcgcctgcca tacctcgccc tccggcgcgc
     2281 cacgggtgcc gggctcgccg gcgatgccgc cgccgtggcc gcgcaggtcg agcggctcgt
     2341 cggccccgat gtgctgttcg tcgacgacct ccactgggtc gatgtcgaca gccaggcggt
     2401 cattgcgctg cttgcggatc ggattctcat cgtggcggcg attcgcaccg gtgatccggg
     2461 tgctgcggcg gcgctcgagc tcgtccaacg cagcgggagc gttctcatca cgctcgatgg
     2521 gctgggcgac gcggccgcgg cccgactcat ccagcgagcg cggcccgagg cgtccgagcg
     2581 tgtcgcggcg gggatcgtcc ggcaggccgg cggcaatccc ctgctcctcg aggagcttgc
     2641 cctcggcggc gaggcgtcga cggcgctcga gcgagcactc gtgagccgct tcgaacggct
     2701 cgacagggac gcgcaggaat cgctcgagct cctcgccgtc gccgaccgcg cgctgccccg
     2761 ggatgctgcc ggaccgggcg cggccgcagc cctcgaggcc ggcctggcgc ggcaaacgcc
     2821 ggacgggatg gctattcgcc atcagctcgt cgccgatgcc atccaggagc agctgaccct
     2881 cgaccgtcgg gcagcgctgc acgtccagct tgctgcactc gcgaccgacc cggtcgaaca
     2941 ggcccggcac ctggttgctg gaggccgcgc cgccgaggcg gaggcgatgg ccctcgaagc
     3001 gattggttcg accatcgacg cgcgccgccg cgcgacgctc ctcgaggttg ccgccgaagc
     3061 gggcggcgcc gggtcccggc tcgacctccg actgcccgct gcgcgggccc tggacgagct
     3121 gtccgattgg cccggcgtgc gccgcgtcct gggcgtgcct gccgagggca gcgccgagga
     3181 gctctgcgag cgtgacgtga tcatcgccca cgcgacattc gccatgggcg atccggtcgg
     3241 tgcgcgtcga tccctcgacg atgcggcaga acgcggggct gccccgcagt ccgaggccgc
     3301 agcccgcctg gccatcgagg aggcgacgta cctcgtcaac gtcgagggcc gcgttgcgga
     3361 ggcaatcgcc cggcttgccg gcgtgctcag cggcaatcct gcccccgaga cggtccgcga
     3421 cgcgagcgtc ctccgggcct gcatcctcct cctcgcgacc ggcgagggag atcccggcgt
     3481 gatcggcgcg ggcctcgagg aggcctttgc cgccggtcga taccggacgg caaccgatcg
     3541 agcgcgggtg ctccagtacg cgatgctgat gggcgtcagc acggaggagg ccctgggctt
     3601 cctgctcgcg cagcacaggc ggttcgacgc cgccggcgtc ggggtggccg cgctggagtt
     3661 cctcgcggat gccgtcgtcg ccgccgtgct cgccggacat ctcgagcagg cgctgctcct
     3721 cgccgacgag ctcctcgagc agcctgcccc gccacgcgcc gtgcagacgg ctgcaatcca
     3781 tcgggcccag gcattgaccc tggcgggacg gtttgaccag gcggagtcga cgctggcgga
     3841 ggcgctgcgt gtggcctcgc ctgactggtt cgggcggggc ctcggcctcg ccgttcaggc
     3901 cgagctcgcg tactggagcg ggcgctacga cgcggcggag acgctcgcgc tggcggcgct
     3961 cggcgtcccc gcaccggtcc cggttgcgca cgtgaacgtg ctgctcaccc aggcgtgggt
     4021 gcggaccgag ctcgggcacg atcccggcgg gccgctcgcg gtcgacctca cgccttcgct
     4081 tgccggcgcc cgcaacgagg tggacggcct ccgggcgtgg catgccggcg acatggcggc
     4141 tgctcggact gcgtttgtcg aggccggtcg ccagtgggcg gcattccatg tcccgcacta
     4201 cctcgtttgt cgctgggcgg ccgcggaatc gctgcggcgg agcggcgatg tcgaggctgc
     4261 ggcggccgag ctcgccgacg tgctggccga ggcgacacgt gcaggcatgg aaccggtcgc
     4321 ggcgcgaatc aggcgctcac tccgcctcgc cggggtcagg tcctcggccg agcctcgcgg
     4381 tcacccatcc gacagcctcg gcctcacccc gcgagagcgc gagctggtcg ccctagtgga
     4441 gcaagggctc accaacgtcg agatcgcacg gcggctcggg ctcggccggc cgaccgtcac
     4501 ccgcatcctg ggatcggcga tgacgaagct cggcgtcggt tcccgagcgc agatggtggc
     4561 ccggctcggg agttgatgcc ggcgcctagg tcgcgtgcgc ctcgaacgtg cccctgatcc
     4621 gtccgtcggt tgcataggtg ccgtcggcct tcgtcacgat cgagttcggg cagtcgaagg
     4681 caccggatgc gttggtatcg gacagctgca ggttgctcat ggtgcaagcc gtcgcgctga
     4741 ctaggccgtc atcggacacg tagctgacca gccatttcgt ggaatcgctc aggtccggga
     4801 agatgctcac gatctcggtt gccgagctgt cggtcctggt gaattggagg ctgacgccgg
     4861 ccggcccgct gaagcgactg ccggcaccga agaacggcag ctcgccggac ttctgcacgg
     4921 ggccgctgat ctcgaagttg accttcccac tgccgttgcc cgggaggacg ccgccgccgc
     4981 ctccgtccgg cgtcgcgacc ggcgcctgcg tcggcgttcc ctggccgccg ccggggttcg
     5041 ccgtgggcgt cacgccgtcc gggcccgccg ttggcggggc cgcgccgccg cacgccgcga
     5101 cgagcagccc ggtgatcgcg aacgagacga ctgtcctcgg gaaccgcatc acatcctcct
     5161 gtcggggata ccgccgtgtc gtccacgatg cgggccggca ggccggctcg tcatcgtcaa
     5221 tctacgccaa cctgcaaccc tgccggcggt ggccaatccg cttcgaacac gagctcgagc
     5281 ccgtcgatct cgtccatctg cgagcccgtc tggctgaagc cgaactcggc gatgagcccg
     5341 agtgaagccg cgttcgtggg cgagacggag gcgatgaagc gacgaatgcc atgggtcgtc
     5401 gccgcccaat cgaacatcgc tcggacggct tcccgggcat agccctggcg ccggaacccg
     5461 ggttcgactc ggtagcccac ctcgaccctc ccctgcgcgt caggcgcacc gtggaagccg
     5521 atcgtcccga tcacgcaacg cgcacccgtc tcgtcgttga gcaccatggc ccgtccgagc
     5581 cactccagga tcgacgggtc gacggccagc tgggtgagcc ggtagcggag gaagtccacg
     5641 aggtcctccg acatcccgtc gggcacggcg gccccgatct cgcgcgatgc tgcctcgagg
     5701 tcatcggcga cgagcgcgtg catgaacggg atcgacatcg agacgagctc caggcgcggc
     5761 gtgcggatcg acgggatcgg ctcggcgtcc gcctcagccg gcacgcgggc tccgatgggc
     5821 caggaactcg agcaggtcca gcttcgtgac cacgcccgcc ggccggccgg cgctgaccgc
     5881 gacgacggcc gccgccgcgc cggagagcag cgcaaacgcg tcatcgagcg acgcgccgag
     5941 atcgatggtc ggcaacggct tgtccatgac ctcgccgacg gtccgctcga cgacggtcgg
     6001 gtcgcggtag gcgcggtcga gcaggctctt ttcgctgacc gagccgacga tcccctcgac
     6061 cgcgtcgtcg ttcgggcgct ccgagaccgg cagctggctg atcccgtaca gctgcagcag
     6121 gtcgatcgcc gcgcccaccc ggtccgtcgt ccgcgccagg acgacggcgg gcatgcccgg
     6181 ctcggggtgg cgcgcgcgca ggagctcatc gatccggatg accccgccgg tcgtcgagag
     6241 caggccgttg gcccggagcc attcgtcgtt gtacagcttc gagaggtagt tgcggccacc
     6301 gtccgggaag atcacgacca tcacggcctc gctggccgct gcgccacgct cgtgcatgat
     6361 ccggcgcgcc tcgtcgagcg cggccacgac ggccgtgccg cacgactcgc ccgcgaggat
     6421 gccctcctcg cgcgtgatcc ggcgcgccat cgcgaatgca tcgcgatccg acacgcgcac
     6481 ccagcggtcg accatcgccg cgtcgtacgt gcccgggaag aagtcctcgc ccacgccctc
     6541 ggtcaggtac ggtcgggcga cgtcgccgga cagcaccgag ccctccgggt ccgccccgac
     6601 gaccacgatg tccgccttct gcgtgcgcag gtagcgcgcg gcgcccgtga tcgtgccgcc
     6661 ggtgccgacg ctggccacga aatgcgtgat ccggccctcg gtctgctccc agagctcggg
     6721 accggtcgtg gcttcgtggg cggtcgggtt ctcct
//
LOCUS       DATBSW010000237         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_375134, whole genome shotgun sequence.
ACCESSION   DATBSW010000237 DATBSW010000000
VERSION     DATBSW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375134"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VIF63_06590"
     CDS             complement(<1..560)
                     /locus_tag="VIF63_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239084.1"
                     /translation="MSRYVGLYPRAWRERYGEELQAVLEAERVGLRARLDLLRGAVDA
                     HLHPPVPSPLPVAAAVTASALAVAHAVALAAQPVPTDWPGYLDDALPLIIASVVALVP
                     ALIGLWLKLGDHDGAIGRLGIVLAIGGHLAWLATLLAALTHVAYGPITALAATVAMAG
                     TAALGIALVGHARLLLGVLVAGAALSG"
     gene            complement(557..1237)
                     /locus_tag="VIF63_06595"
     CDS             complement(557..1237)
                     /locus_tag="VIF63_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8239085.1"
                     /translation="MDERDPSLRREEAALVGARIRALRQDPVAGISRRTLLRRSLGLG
                     VGLWLTEVGVGSISFLWNAVGGGQAKVRVGTIEQVAGIAPNLPFYAGFPMYLQAARSF
                     VVLVDPSVRRFLPGVDEKGAGSLNVRALSQVCPHLGCRPNPCIEDFWFHCPCHQSRYD
                     RLGIKPTGKGGFYGPAERGMDRFAVEVDADGVLTIDTSHVTLGPLPVTLGQPGVIPPL
                     VPHERACS"
     gene            1379..2158
                     /locus_tag="VIF63_06600"
     CDS             1379..2158
                     /locus_tag="VIF63_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239086.1"
                     /translation="MTTTGRWDRAALVPVRRGERRAEVMALPAGQPTVDELFTFMRDA
                     ERRFETLLMRIEERTYGARGEQVATLDVALRHPGHARVVTTARGGATTGSHELWISDG
                     EVVRTYSAEHRLGTSRPVRNRVRGLDPKQYPGRSTVYEPITALPMETLAELFVHPAGY
                     CQNVLATGPCWISGTDRVAGRAAIVLECDHPRAVEQSVDRPDFHIQVTVDRIDGLISR
                     LVESVGGEVTRVAQIVHLDPDAGLPSTTFDFEFPEGTTLLY"
     gene            2269..>2748
                     /locus_tag="VIF63_06605"
     CDS             2269..>2748
                     /locus_tag="VIF63_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8239087.1"
                     /translation="MASREEAIRQDTADLHGLGYAQELLRSMGGFSNFAISFSIISIL
                     TGAVILFNYGLNLAGPAAVGLGWPLVTIFTLMIAATMAEIASAYPTAGGLYYWASKLR
                     NKDWGWWTAWLNLGGQISIVAGINYAAAFYLTATIINPIVGGSFDPAADTVGVQNAIW
                     "
BASE COUNT          442 a          989 c          933 g          384 t
ORIGIN      
        1 ccggacagcg cggcgccggc gacgaggacg ccgagcagga gccgggcgtg gccgacgagc
       61 gcaatgccga gcgcggcagt gccggccatc gccacggtcg cggcgagcgc tgtgatcggc
      121 ccgtaggcca cgtgggtcag cgcggcgagc agcgttgcaa gccacgcgag gtgtcccccg
      181 atcgcgagca cgatcccgag gcggccgatc gcgccgtcgt ggtcgccgag cttcagccac
      241 aggccgatca acgccggcac gagcgcgacc acgctggcga tgatcagcgg cagggcatcg
      301 tcgagatagc ccggccagtc ggtgggcacc ggctgcgcgg caagggcgac ggcgtgggcc
      361 accgcgaggg cgctcgcggt cacggcggca gcgacgggca gcggtgacgg gaccggcgga
      421 tggaggtggg catcgaccgc cccgcggagc aggtcaagcc gggcgcggag cccgacccgc
      481 tctgcctcga ggaccgcctg gagctcctcg ccgtagcgct cccgccatgc ccgggggtac
      541 aggcccacgt agcggctcac gagcacgctc gttcatgcgg gacgagcggc ggaatcacgc
      601 ccggctgccc cagcgtgacc ggcagcggac caagggtcac gtggctcgtg tcgatcgtga
      661 gcacgccatc cgcgtcgacc tccaccgcga accggtccat gccccgctcg gccggcccgt
      721 agaacccacc cttgcccgtc ggcttgatgc cgagccgatc gtatcgcgac tggtggcacg
      781 gacagtggaa ccagaagtct tcgatgcacg ggttcggccg gcagccgagg tgcggacaga
      841 cctgggacag ggctcggacg ttgaggctcc cggcaccctt ctcgtcgacg ccggggagga
      901 agcgacggac gctcgggtcg acaagcacga cgaacgacct cgccgcctgc aggtacatcg
      961 ggaagcccgc atagaacggc aggttcggcg cgatcccggc gacctgctcg atcgtcccga
     1021 cgcgcacctt cgcctgcccg ccaccgacgg cgttccacag gaagctgatc gacccgacgc
     1081 ccacctcggt gagccacagc ccgacaccga ggccgaggga ccggcgcagc agggtccgac
     1141 ggctgatgcc cgcgacgggg tcctggcgga gcgcccgaat gcgggcgccg acgagggctg
     1201 cctcctcgcg ccgcaggctg ggatctcgct cgtccacgga cgtgacctcg actatcgctc
     1261 accgttagag cgctgttcga tatatcggac tccgatatac acccgtcagc ggcacggcgt
     1321 caagcgatgg gccgcgtcgc cgcgcaggaa ccaccgtgcc gctggctaca atccggccat
     1381 gaccacgacg ggccgctggg atcgcgccgc gctcgtgccg gtccggcgcg gcgagcgccg
     1441 cgccgaggtg atggcgctgc ctgccggcca gccgaccgtc gacgagctgt tcacgttcat
     1501 gcgggacgcc gagcgcaggt tcgagacgct gctcatgcgc atcgaggagc gcacgtacgg
     1561 cgcccgcggc gagcaggtcg ccaccctcga cgtcgccctg cgccatcccg gccatgcacg
     1621 cgtggtcacg acggcccgcg gcggggccac gacgggcagc cacgagctgt ggatctccga
     1681 cggcgaggtc gttcgcacct actcggcgga gcatcgcctc ggcaccagcc ggccggttcg
     1741 caaccgcgtc cgcggcctcg atccaaagca atatccaggc cgctccacgg tgtacgagcc
     1801 aatcaccgcc ttgcccatgg agacgctcgc cgagctgttc gtccatcccg ccggctactg
     1861 ccagaacgtg ctcgccaccg gcccgtgctg gatctcgggc accgaccggg tcgcgggccg
     1921 cgcggccatc gtgctcgagt gcgaccaccc gcgcgcggtg gagcagtcgg tcgaccgtcc
     1981 cgacttccac atccaggtga cggtcgatcg catcgacggc ctcatcagcc gcctcgtgga
     2041 gtccgtcggc ggcgaggtca cccgggtcgc ccagatcgtc cacctcgacc cggatgcggg
     2101 cctgccctcg accaccttcg acttcgagtt ccccgagggc acgacgctcc tctactgacc
     2161 ggcgaccgac cgcctgcggc ctcgctgaaa tgtgcgaatc acgcgaattt tcgcgcgtat
     2221 gatcgcgcca attcgcgcgc agaagcgtcg ctctggaggg aggtcgcaat ggcgagtcgt
     2281 gaggaggcca tcaggcagga caccgcagac ctgcacggcc ttggctatgc ccaggagctg
     2341 ctccgcagca tgggtgggtt cagcaacttc gcgatcagct tctcgatcat ctcgatcctg
     2401 accggggcgg tcatcctctt caactacggg ctcaatctgg cgggaccggc cgctgtcgga
     2461 ctcggctggc cgctcgtcac gatcttcacc ttgatgatcg cggccaccat ggccgagatc
     2521 gcctcggcat atccgaccgc cggcggcctg tattactggg ccagcaagct acgaaacaag
     2581 gattggggct ggtggaccgc ctggctcaat ctcggcgggc agatttcgat cgtcgccggc
     2641 atcaactacg ccgcagcctt ctacctcact gccacgatca tcaatccgat cgtcggcggc
     2701 agcttcgatc ccgcggcgga cacggtcgga gtccagaacg cgatctgg
//
LOCUS       DATBSW010000238         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1079815, whole genome shotgun sequence.
ACCESSION   DATBSW010000238 DATBSW010000000
VERSION     DATBSW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079815"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VIF63_06610"
     CDS             <1..246
                     /locus_tag="VIF63_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239088.1"
                     /translation="EVLANAGAAQLIDDDAFDADALLAAVAVLENPTRHATMAAASRS
                     LGRPGAAGAVATLLLALAERRALPDAAAVARIAAGAL"
     gene            243..1259
                     /gene="murB"
                     /locus_tag="VIF63_06615"
     CDS             243..1259
                     /gene="murB"
                     /locus_tag="VIF63_06615"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8239089.1"
                     /translation="MTSPGATTNGTTTTAGPATALDLTALGADIARRVGVKAERSAPL
                     ARFTTMRVGGAADLLATAHNAFELRALVRFARSRDLPLTLLGRGSNSVISDRGVRGLV
                     VLVKAEGSRIDGNAFVAEAGVPMARAATETQKAGLTGLEFGLAIPGAVGGAVWANAGA
                     HGSDVKQILESADVLLADGTEARVPAAELGLGYRESRFKHPAPGAPAEVVVGARFRLE
                     PASADEIKERLDEIRHWRQAHQPLGIPSAGSTFRNPAEGPSAGALIDGAGLKGLQEGG
                     ATVSEKHANFVVNDRKGTAADVRRLVDIVRAKVLAASAVTLEPEIVFVGDWTGWPWAP
                     EDHR"
     gene            1256..>2023
                     /locus_tag="VIF63_06620"
     CDS             1256..>2023
                     /locus_tag="VIF63_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239090.1"
                     /translation="MTDRPAAADRAVTYPPVVVLLGGPSAEHDVSVVSGTAIAAALLD
                     AGLAAQQLLIDLDGRWWWLPEDHRRGDKPASAYDDPTTLDADGPHAVGAALDRLAAAR
                     PAPVVFIALHGPFGEDGTVQALLEAAGLAYTGAGVAASALGMDKIAQKRIWRGLGLPV
                     IDWIEVRGASWTANRALVLTQLAAFATGDQRLMVKPARLGSSVGMTLAHTAEEREAAL
                     DLAFRYDDLAIVERYLARARDLEVSVIGTEAPLGVYGP"
BASE COUNT          270 a          730 c          761 g          262 t
ORIGIN      
        1 gaggtgctcg cgaacgcggg cgcggcgcag ctgatcgacg acgatgcgtt cgacgcggac
       61 gcgctgcttg ccgcggtcgc ggtgctcgag aatcccacgc ggcacgcgac gatggccgcg
      121 gcgtcgcgct cactgggccg cccgggtgcg gccggcgcgg tggccacgct gctgctcgcc
      181 ctggccgagc gacgtgcgtt gccggacgcg gcggcggtgg ccagaatcgc ggctggtgcc
      241 ctgtgaccag cccgggcgca accacgaacg gaacgaccac gaccgccggg cccgcgacgg
      301 cgctcgacct cacggccctc ggcgccgata tcgcccgacg cgtcggcgtg aaggccgagc
      361 gcagcgcgcc gctggcgcgc ttcaccacga tgcgcgtcgg gggcgcggcg gacctgctcg
      421 cgaccgcgca caacgccttc gagctgcggg cgctggtgcg gttcgcgcgc tcgcgcgacc
      481 tgccgctgac cctgctcggg cgggggagca acagcgtgat ctccgatcgc ggcgtgcgcg
      541 ggctggtcgt cctcgtcaag gccgagggca gcaggatcga tgggaatgcg ttcgtcgccg
      601 aggcaggggt gccgatggca cgtgctgcca cggagaccca gaaggccggg ctgacgggcc
      661 tcgagttcgg gctcgcgatc ccgggcgcgg tcggtggcgc ggtctgggcg aatgccggcg
      721 cgcacggctc ggacgtcaaa cagattctcg aatcggccga cgtcctgctt gccgacggca
      781 cggaggcgcg cgtgcccgcc gccgagctcg gcctcggcta tcgcgagagc cggttcaagc
      841 acccggcgcc cggtgcgccc gcggaggtgg tggtcggggc gcgcttccgc ctcgagccgg
      901 cctctgccga cgagatcaag gagcgcctcg acgagatccg gcactggcgc caggcgcacc
      961 agccgctcgg catcccgtcg gcaggaagca cgttccgcaa cccggccgag ggtccgtctg
     1021 ccggcgcctt gatcgatggc gcgggcctca aggggctcca ggaaggcggg gcgaccgtct
     1081 ccgagaagca cgccaacttc gtcgtcaacg acaggaaggg aacggcagcg gacgttcgcc
     1141 gcctcgtcga catcgtccgg gcgaaggtgc tcgcggcgag cgccgtcacg ctcgagcccg
     1201 agatcgtctt cgtcggcgac tggacgggct ggccgtgggc cccagaggac caccgatgac
     1261 cgaccgacca gccgctgccg accgcgccgt cacgtacccg ccggtcgtcg tcctgctcgg
     1321 cggcccgtcg gccgagcacg acgtctcggt cgtctcgggc acggccatcg ccgcggcgtt
     1381 gctcgacgcc ggcctggccg cccagcagct gctcatcgac ctcgacggtc gctggtggtg
     1441 gctgcccgag gaccaccgtc gaggcgacaa gccggccagc gcctacgacg acccgaccac
     1501 gctggacgcg gatggccccc acgccgtggg cgctgcgctc gaccgactcg ccgcagcgcg
     1561 gcccgcgccg gtggtgttca tcgcgctgca cggaccgttc ggcgaggacg gcaccgtcca
     1621 ggcgctgctc gaggcggcgg gcctggcgta caccggcgcc ggtgtcgccg cgtccgccct
     1681 gggcatggac aagatcgccc agaagcggat ctggcgcggc ctcgggctgc cggtgatcga
     1741 ctggatcgag gtgcgtggcg cgagctggac ggcgaatcga gcgctcgtgc tcacccagct
     1801 cgcggcgttc gccaccggcg accaacgcct gatggtcaag ccggcgcggc tgggatcgtc
     1861 ggtggggatg accctcgccc acaccgccga ggaacgtgag gccgcgctgg acctcgcctt
     1921 ccgctacgac gacctcgcga tcgtcgagcg gtacctcgcg agggctcgcg atctcgaggt
     1981 ctcggtgatc gggacggaag cgccgctggg cgtatacggg ccg
//
LOCUS       DATBSW010000239         7479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_269684, whole genome shotgun sequence.
ACCESSION   DATBSW010000239 DATBSW010000000
VERSION     DATBSW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269684"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VIF63_06625"
     CDS             complement(<1..935)
                     /locus_tag="VIF63_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8239091.1"
                     /translation="MLIKTPGEPKEAHETRLLATAPPLLDMDAYVEEFGRSVVPAYRQ
                     GIADLELPADAGLARSVIPPGTAATRDFSQLAPRIPELVAERCVGCMACVNACPDTAI
                     LGTVIPLTQLDARIKAFAEGRPDPTRAAATARGHFVDTQKYGEVPARRGLERGAFGIF
                     VDPGHCKGCGECVEVCAALGHDALFMIDKVADESLAPAHGSVAPAAAVADAALAASPV
                     APEDAAPALAGESTLDRFRRDMAFYRSLPPTPPEYRNEKALADLMLGEFAFGYVGGAG
                     SCAGCGEATAIRMLVAATRQVHGPESMGIVAATGC"
     gene            complement(941..1414)
                     /locus_tag="VIF63_06630"
     CDS             complement(941..1414)
                     /locus_tag="VIF63_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8239092.1"
                     /translation="MNGTLDRDHHGPIVADHMAASPILVRADASLSEAVALMDRHHVH
                     GLPVIDDEGALIGVLSQTDLNRARSTEYLWANWPGLAVRHLMTSPAITVHKSTPLLVA
                     ARRMEEEHIHRLVVVEDGDDGWPIGVLSMTDLIHALAGETAASASRPSYRPESAE"
     gene            complement(1411..3375)
                     /locus_tag="VIF63_06635"
     CDS             complement(1411..3375)
                     /locus_tag="VIF63_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY8239093.1"
                     /translation="MRSTATAPAPAFPGIPAIVDGSEAVAHVESRVAEVACVYPITPS
                     TTMAAIYQAAVADGRTDLWGTPLRFIEPESEHSSASAAEGAALAGARVTNFTAGQGLV
                     LMKEVLYVISGKRLPVVFHVGARALTSQALNIHAGHDDVMAIADCGWGILFARNAQEA
                     ADLAAIARRTAEATETPFIVAQDGFLTTHTLENVLLPEDELLREFLGDPRDHVRDLFD
                     PAEALMTGVVQNQDSYMKGRIGQRAYTDRTLAALEDAMGEWAARTGRALAPVATYRTG
                     DAEEILVAMGTIADTAIAVVDRLRDQGRAVGCVALTAFRPFPTDALVAALAGARAVGV
                     VERTDEPLAVANPLTREVRAALFDAAAAAAMPRVRSYVAGLGSRDVSAGDLVAVFDDI
                     ADGSAPPAPVRFLGIRHPLALAAAQVDVRPPGAYSMRGHSIGGFGSVTTNKLVATLVG
                     ELFDKQVQAYPRYGSEKKGLPTTYYLTIADLPIRTHAELDRVDFVPLHDVSAFGLGAP
                     LAGLVDGGDLFIQSPLTSPEAIWASIPAAARAEILARLIRVTALDTAGLARRHSPRSD
                     LQIRMQGVALVGVFLRVSPFAAAAGLGRSQLLEAVGDRLGRFFGKRGASVVRANLAVI
                     AEAWDGLIDVTAAITGLDHVPSASKQGVLL"
     gene            complement(3456..3950)
                     /locus_tag="VIF63_06640"
     CDS             complement(3456..3950)
                     /locus_tag="VIF63_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239094.1"
                     /translation="MTAPAPILRLLVCGSADRGDDGAALNAVAHVLPRLALDVRQQLE
                     VRRCVQLDPIDVISIAAGEACLVLDTVVGVVPGEVVALDLDDLADRATVAPRSSHALP
                     IDQTLGIARACRGGLPRGMFVGIGGKWFGFGQTRSRALRDGMVAFESVIEAAILNLTA
                     PVPA"
     gene            complement(3947..5335)
                     /locus_tag="VIF63_06645"
     CDS             complement(3947..5335)
                     /locus_tag="VIF63_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY8239095.1"
                     /translation="MHELEGGGFGGVTPVPPQSGSATPAGTSRSGDLRFMVQNLTRVE
                     GEGSLHLLVRDGLVAEARLQIFEAPRYFEALVVGRTPDEVLDIVSRICGICPVAYQMS
                     AVHAFEDLFGVEVDPSTRALRRLMYCGEWIESHALHIYLLHAPDFLGYPSAVELAKDH
                     GQLVKDGLRLKQIGNHLLALLGGRAIHPVSVRVGGWTRAPRRAQLLAMRPALEEGLRL
                     ARATVDVALSLAPPEFEREPRLVALHSPNEYPMNEGRIISNDGLQLAPQAWGEAFHEV
                     QVPWSNALQSRGSDNLPYLLGPAARIQLDHEQLHPIAREALDASGALESIRLNPYRSI
                     VARAIELVHATADALDIVDGYTPPELPKVDWEARPGTAAWATEAPRGLLFHRYEVGED
                     GKIAAATIVPPTSQNQAAIEADLAAFAPRVLDLPMEAATHALEQLIRSYDPCISCATH
                     FLDLTVRHEVGP"
     gene            complement(5328..6260)
                     /locus_tag="VIF63_06650"
     CDS             complement(5328..6260)
                     /locus_tag="VIF63_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239096.1"
                     /translation="MQVPQGSTQVSARATAVSRPRVGVIKLASCDGCQLTILDLEDEL
                     LALTDRFDFVEFAEATSRRSSGPFDVLFVEGSISAPEQVAEITRLRAATKTLVTIGAC
                     ATAGGIQALRTAAEHEAFRAAVYPAPAFVESLAVASPIANYVTVDAELRGCPIDPHQL
                     VELLTAVTTGRRPQLPDQAVCLECKRRGWECVVVTRGEPCLGPVTQTGCGAICPGMGR
                     GCYGCFGPREGANVDGLANWFVDGPALGGIAAPDVGRLFAGFTGAAAPFRAVTDRLGG
                     RPQIPLRPATAHGPEHSAHTPPRWGSDVREDPDA"
     gene            complement(6260..>7479)
                     /locus_tag="VIF63_06655"
     CDS             complement(6260..>7479)
                     /locus_tag="VIF63_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8239097.1"
                     /translation="LACANCTLVCPTCFCTGTSITSDLDGRESSAARQWDSCFTAGFA
                     QVAGGGSFRPKHADRYRQWLTHKFGTWFDQFGSSGCVGCGRCVTFCPVGIDVREELAI
                     IAGSSTATAPVAPPFPIKGTRVLAMAPAALQPGSIRIDYVTATIAEVRPETADTSTLR
                     LVTDDAALLGARPGQFVMVAVPAFAIPPISISRINPDGLELTIRAAGPATSFLTRLRP
                     GAQLGLRGPLGRAWPIEDAYGRDVAIVAGGIGLAPLRGLLDQVLSERERFSSVRLYLG
                     ARTPRDRLFVAEMDALAAAGLDIRATVDRAGPEWLGRVGIITDIFRNAKPTGQDVTAF
                     VCGPERMMTAVVDVLGDLGVPREHTWLTLERRMECGVGLCGHCQLGTRFVCRDGPVFS
                     VAELGDDLRREGL"
BASE COUNT         1129 a         2553 c         2784 g         1013 t
ORIGIN      
        1 ttgcagccgg tggcggcgac gatgcccatg gactccggac cgtgcacctg ccgcgtggcg
       61 gcgacgagca tccggatcgc cgttgcctcg ccgcagccgg cgcaggagcc ggcgcccccg
      121 acgtagccga acgcgaactc gccgagcatc aggtcggcga gggccttctc gttgcggtac
      181 tccggcggcg tgggcgggag ggaccggtag aaggccatgt cgcgacggaa tcggtcgagc
      241 gtcgactcgc cggccagggc cggagcggca tcttccggcg cgaccgggga ggctgcaaga
      301 gcggcatccg ccacggcagc tgcgggcgcg accgacccat gtgctggcgc gagcgactcg
      361 tccgcgacct tgtcgatcat gaacagggca tcgtggccga gcgccgcaca cacttcgacg
      421 cactcgccac agcccttgca gtggcctgga tcgacgaaga tgccgaaggc gccgcgctcg
      481 agcccgcgtc gcgcgggcac ctcaccgtat ttctgggtgt ccacgaagtg cccgcgagcg
      541 gtcgccgcag cgcgagtggg atccggcctg ccctcggcga acgccttgat gcgcgcatcg
      601 agctgcgtca gcgggataac cgtgccgagg atggccgtgt ccggacatgc gttgacgcac
      661 gccatgcagc cgacgcagcg ctcggcgacg agctccggga ttcgcggtgc gagttggctg
      721 aagtcgcgtg tcgccgccgt ccccggcggg atcacggacc tggccagacc agcgtccgcg
      781 ggcagctcga ggtcggcgat tccctggcgg tacgccggca cgaccgagcg cccgaactcc
      841 tcgacgtagg cgtccatgtc gagcagcggc ggcgcggttg cgaggaggcg cgtctcgtgc
      901 gcctccttcg gctcgcccgg cgtcttgatc agcatctcgc tcactccgcc gactccgggc
      961 ggtacgacgg cctggacgcg ctcgccgccg tctcgccggc gagggcatgg atgaggtccg
     1021 tcatcgacag gacgccgatc ggccagccgt cgtcgccgtc ctcgacgacg acgaggcgat
     1081 ggatgtgctc ctcctccatc cgacgcgcgg cgacgaggag cggggtcgac ttgtggaccg
     1141 tgatcgccgg gctcgtcatg aggtggcgca ccgcgaggcc gggccagttc gcccagagat
     1201 actccgtgct ccgggcgcga ttgagatcgg tctgcgacag cacgccgatc agcgcgccct
     1261 cgtcatcgat gacgggcagg ccgtggacgt ggtgtcgatc catgagcgcg acagcctcgg
     1321 acagggaggc gtctgcacgg acgaggatcg ggctcgcggc catgtggtcg gccacgatcg
     1381 ggccgtggtg atcacgatcg agcgtgccgt tcacaggagc accccttgct tgctggccga
     1441 cgggacgtgg tcgaggccgg tgatcgcggc ggtaacgtcg atcagcccgt cccacgcctc
     1501 ggcgatgacg gcgagattgg ctcgaaccac gcttgcgccg cgcttgccga agaaccgccc
     1561 gagtcggtcg cccaccgcct cgagcagctg gctccggccc agccctgcgg ccgcggcgaa
     1621 gggcgacact cgcaggaaga ccccgacgag tgcgacgccc tgcatccgga tctggaggtc
     1681 ggaccgcggc gagtggcgcc gcgccagccc ggcggtgtcg agggcagtca cgcgaatcag
     1741 acgggcgaga atctccgcgc gcgcagcggc cgggatggac gcccagatcg cctccggcga
     1801 ggtgagcggc gactggatga agaggtctcc gccgtcgacg agccctgcga gcggagcgcc
     1861 gaggccgaag gctgacacgt cgtggagcgg cacgaagtcg acgcggtcga gctcggcgtg
     1921 cgttcggatc ggcaagtcgg cgatcgtcag gtagtacgtc gtcggcagac ccttcttctc
     1981 ggatccgtag cgcggataag cctggacctg cttgtcgaac agctcgccga ccagcgtcgc
     2041 gacgagcttg ttggtggtga cgctcccgaa cccgccgatc gagtgtccac gcatcgagta
     2101 cgcccccggt gggcggacgt cgacctgggc cgcggcaagg gccagcggat ggcgaatgcc
     2161 aaggaaacgg acgggagcag gcggcgcact gccgtccgcg atgtcgtcga agaccgcgac
     2221 gaggtcacct gccgagacgt cgcgcgatcc cagaccggcc acgtaggaac gcacccgggg
     2281 catcgccgcc gccgcggcag catcgaataa tgcggcccgg acctcccgag tgagggggtt
     2341 cgcgacggcc aggggctcgt cggtccgctc gacgacgccg acagctcgcg cgccggccag
     2401 cgccgcgacg agggcgtcgg tcgggaatgg gcggaaggcg gtcagcgcga cgcaacctac
     2461 ggctcggccc tggtcccgga gacgatcgac gaccgcaatc gccgtatccg cgatcgtgcc
     2521 cattgcgacg aggatttcct ccgcatcgcc ggtgcgatag gtcgcgacgg gtgccagcgc
     2581 ccggcctgtt cgcgctgccc actcgcccat cgcgtcttcg agcgcggcga gggttcggtc
     2641 ggtatacgcg cgctggccga tgcggccctt catgtagctg tcctggttct ggacgacacc
     2701 ggtcatcagt gcctcggccg gatcgaacag gtcccggacg tggtcgcgtg ggtcgcccag
     2761 gaactcccgg aggagctcgt cctccggcag gaggacgttc tcgagcgtgt gcgttgtcag
     2821 gaagccgtcc tgcgcgacga tgaatggcgt ctcggtcgcc tcggcggtgc gccgggcgat
     2881 ggcggcgagg tcggccgcct cctgcgcgtt gcgcgcgaac aggatccccc agccacagtc
     2941 cgcgatggcc atgacgtcgt cgtggccggc atggatgttc agcgcttggc tcgtcagggc
     3001 gcgggcgccg acgtggaaca ccacggggag gcgcttgccg ctgatgacgt acaggacttc
     3061 cttcatcagg acgagaccct ggccggcggt gaagttggtg acccgggcgc cggcgagtgc
     3121 ggcgccctcc gcggcggagg ccgaggaatg ctccgactcg ggctcgatga accggagcgg
     3181 cgtgccccag aggtccgtcc gaccgtcggc gacagcagcc tggtagatgg cggccatcgt
     3241 cgtcgatggc gtgatcgggt agacgcaggc gacctccgcg acgcggctct cgacgtgggc
     3301 gactgcctcg gagccatcga cgatggccgg aatgccgggg aaggccgggg cgggcgcggt
     3361 ggcggtggat cgcatgtgtc gatggtcgtc gcgcggcgag ccctgacgaa gggacgagtg
     3421 gcccgaaggt ggggtccgga cggccccgtt gggcgtcagg ccggcaccgg cgcggtgaga
     3481 ttcaggatgg ccgcctcgat gacggactcg aacgcgacca tgccgtcacg gagcgcgcga
     3541 gaccgcgtct gcccgaatcc gaaccacttc ccaccgatcc cgacgaacat gcctcgtggc
     3601 agcccgccac ggcaggcgcg ggcgatgccg agggtctggt cgatcgggag cgcgtgggac
     3661 gagcgcggcg ccacggtcgc gcgatccgcg aggtcgtcga ggtcgagcgc caccacctcg
     3721 ccgggcacga cgcccacgac ggtatcgagc acgagacagg cctcgccggc ggcgatgctg
     3781 atgacgtcga tcgggtcgag ctggacgcac cgccggacct cgagctgctg gcgcacgtcc
     3841 agggccagcc gaggcagcac gtgggcgacg gcgttgagcg ccgcgccgtc atccccgcgg
     3901 tcggcgctcc cgcagacgag gaggcgaagg atcggcgcgg gcgcggtcat ggccccacct
     3961 catggcggac ggtcaggtcg aggaagtgcg tcgcgcacga gatgcacggg tcatacgacc
     4021 gaatcagctg ctcgagcgcg tgcgtcgcgg cctccatcgg caggtccagg acccgcggcg
     4081 caaaggccgc caggtctgcc tcgatggctg cctggttctg ggatgtcggt ggcacgatgg
     4141 tggccgccgc gatcttcccg tcctcgccga cctcgtagcg atggaagagg agcccacggg
     4201 gcgcctccgt ggcccatgcg gccgtgcccg gccgcgcttc ccagtcgact ttcggcagct
     4261 ccgggggcgt gtacccatcg acgatgtcga gcgcgtcggc ggtcgcatgg accagctcga
     4321 tcgcccgggc gacgatgctc cgatacgggt tgagccggat cgattcgagg gcgccgctgg
     4381 cgtcgagggc ctcccgcgcg atcgggtgga gctgctcgtg atccagctgg atccgcgccg
     4441 ccggcccgag gagatacggc aggttgtcgc tgccgcgcga ctgcagcgcg ttggaccacg
     4501 ggacctggac ctcgtggaac gcctcgcccc acgcctgcgg cgcgagctgg aggccgtcat
     4561 tcgagatgat ccggccctcg ttcatcgggt actcgttcgg gctgtgcagc gccacgagcc
     4621 gcggctcccg ttcgaactcc ggaggcgcga ggctgagcgc cacgtcgacg gtggcgcggg
     4681 cgaggcgaag gccctcctcg agcgccggtc gcatggccag cagctgggcc cgcctcggcg
     4741 cgcgagtcca gccaccgacg cggacgctca cggggtggat cgcccggccg ccgagcaggg
     4801 ccaggaggtg attgccgatc tgcttgagcc gcagcccgtc cttgacgagc tggccgtgat
     4861 ccttggccag ctcgacggcg ctcgggtagc cgaggaagtc gggcgcgtgc agcaggtaga
     4921 tatgcagcgc gtggctctcg atccattcgc cgcagtacat gagccggcgg agggcgcggg
     4981 tggacgggtc gacctccacc ccgaacaggt cctcgaacgc gtgcaccgcg ctcatctggt
     5041 aagcgacagg acagatccca cagatcctgg agacgatgtc cagcacctca tccggcgtgc
     5101 ggcccacgac gagcgcctcg aagtagcgcg gtgcctcgaa gatctgcagc cgggcctcgg
     5161 cgaccagccc gtcacgcacg agcagatgga gcgagccctc gccctcgaca cgcgtcaggt
     5221 tctgcaccat gaagcgcagg tcaccggagc gcgacgtccc cgcaggcgtg gccgagccgc
     5281 tctggggcgg aacgggcgtc acgccgccga agccgccacc ttcgagctca tgcatcggga
     5341 tcctccctga cgtcgctccc ccatcggggc ggcgtgtggg cgctgtgctc gggaccgtgg
     5401 gcggtcgcag gccggagcgg gatctggggg cgcccgccga ggcgatcggt cacggcgcgg
     5461 aacggggccg ctgcgccggt gaagccggcg aacaggcgcc cgacgtcggg ggccgcgata
     5521 ccgccgaggg ccggcccatc gacgaaccag ttcgccagac cgtcgacgtt cgcgccctcg
     5581 cgcggcccga agcagccgta acagccgcgg cccatgcccg gacagatcgc cccgcagccg
     5641 gtctgcgtga ccggaccgag gcacggctcg ccgcgtgtca cgacaacgca ctcccagccg
     5701 cgccgcttgc actccaggca gacagcctgg tcgggcagct gcgggcgcct gcccgtggtg
     5761 accgcggtga ggagctcgac gagctgatgc gggtcgatcg ggcagccccg cagctcggcg
     5821 tcgacggtga cgtagttcgc gatcggcgag gccacagcga gcgactcgac gaatgccggc
     5881 gccggataga cggcggcgcg gaacgcctcg tgctcggccg cggttcggag tgcctggatt
     5941 ccgccggctg tggcgcaggc gccaatcgtc acgagggtct tcgttgccgc gcggaggcgc
     6001 gtgatctcgg cgacctgctc gggcgcggag atggagccct cgacgaacag cacgtcgaac
     6061 ggcccggagg agcgacgcga cgtcgcctcg gcgaactcga cgaaatcgaa ccggtcggtc
     6121 agggcgagca gctcgtcctc gaggtcgagg atcgtgagct ggcagccatc gcacgaggcg
     6181 agcttgatga ccccgacccg gggccggctc accgcggtgg cgcgcgccga gacctgcgtg
     6241 gaaccctgcg ggacctgcat cacaggcctt cccttcggag gtcgtcgccg agctcggcga
     6301 cggagaagac cggtccgtcg cggcagacga acctcgtccc gagctggcag tggccacaca
     6361 accccacgcc gcactccatg cgccgctcga gggtcagcca cgtgtgctcg cgcgggacgc
     6421 cgaggtcgcc gagcacgtcg acgacggcgg tcatcatccg ttccgggccg cacacgaacg
     6481 cggtgacgtc ctggcccgtt ggctttgcat tccggaagat gtccgtgatg atcccgaccc
     6541 gtccgagcca ctccgggccg gcacggtcca cggtcgcgcg gatgtccagg ccggccgcgg
     6601 cgagggcgtc catctcggcg acgaacaggc ggtcccgggg cgtcctggct ccgaggtaga
     6661 gtcgcaccga cgagaagcgc tcgcgctccg acagcacctg gtcgagcagg ccccgcaggg
     6721 gcgcgagccc gatcccaccg gcgacgatcg cgacgtcccg cccgtacgca tcctcgatcg
     6781 gccacgcccg cccgaggggg ccgcggagcc cgagctgcgc gccgggccgg agccgggtga
     6841 ggaagcttgt cgcgggaccc gccgcgcgga tcgtgagctc caggccgtcc gggttgatgc
     6901 gggagatgga gatcggcggg atggcgaagg cgggcacggc gaccatgacg aactggccgg
     6961 gtcgagcgcc gaggagggcg gcgtcgtcgg tgaccaggcg gagggtcgag gtatcggcgg
     7021 tctccggccg cacctcggcg atggtcgccg tcacgtagtc gatccggatg gagccgggct
     7081 ggagcgcggc gggcgccatc gccaggacgc gcgtgccctt gatcgggaac ggcggcgcga
     7141 ccggcgcggt cgcggtggac gagccggcga tgatcgccag ctcctcgcgg acgtcaatgc
     7201 cgaccgggca gaacgtcacg caccggccgc agccgacgca gccggagctg ccgaactggt
     7261 cgaaccaggt accgaacttg tgggtgagcc actggcggta gcggtcggcg tgcttgggcc
     7321 ggaagctgcc gccgcccgcg acctgcgcga agccggcggt gaagcagctg tcccattgcc
     7381 gcgcggcgga ggattcgcgg ccgtcgagat ccgaggtgat cgacgtgccc gtgcagaagc
     7441 acgtcgggca aacgagggtg cagttcgcgc acgcgagac
//
LOCUS       DATBSW010000240         5549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_22847, whole genome shotgun sequence.
ACCESSION   DATBSW010000240 DATBSW010000000
VERSION     DATBSW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22847"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="rpoD"
                     /locus_tag="VIF63_06660"
     CDS             <1..771
                     /gene="rpoD"
                     /locus_tag="VIF63_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY8239098.1"
                     /translation="DRKGDIVVIGRDAREQLTSANLRLVVSIAKKYIGRGMSFLDLIQ
                     EGNIGLIRAVEKFDYEKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINRLI
                     RVSRGLLQELGREPTVEEIAEAMSRGQEVVVTPEKVREIIKVSQEPVSLETPIGEEED
                     SHLGDFIEDRGALAPAEAASHQLLKEQVEAVLDSLTGRERRVLQLRFGLEDGRARTLE
                     EVGKEFNVTRERIRQIEAKALRKLRHPSRSRKLKDYLE"
     gene            complement(1157..1771)
                     /locus_tag="VIF63_06665"
     CDS             complement(1157..1771)
                     /locus_tag="VIF63_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239099.1"
                     /translation="MTKLCADLEEARLDGGLSYVALGRPLGLSGVQVARICQGRSPDV
                     SIARMAQLYALVGLDLSARGFPAGPPLRDAGHLALLARLRARLGPSLRWRTEVPVTAQ
                     GETASMDSRAWDATISGPGWTIGVEAETHVRDVQALERRLALKLRDGHAATAILLLSD
                     TRHHRTLLEVAGPALRETFPIPTRSALRSLGRGEAVTGNGLVVL"
     gene            1954..3768
                     /locus_tag="VIF63_06670"
     CDS             1954..3768
                     /locus_tag="VIF63_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239100.1"
                     /translation="MGRVIGERIGALRTRRPRRDIAARARPAVAARRAIAVRRVLAVL
                     AAGVLALGSIVAPVSAGTPPGADPLLEILKVYAEVEGLDVVVEGNIVRLEDPLGDVSG
                     NDAGGNDIASAGYAWLPMVPAGLLDGPFKCGEAKVACDNAATDRYAHGAWLFHGRRAM
                     PYGDVPAGARVEFGPQLALMQYPAAPIEGDNPFGGTSHNVIARIQGGTGEMHYFGYDG
                     TTFPEFNTQSRATWTATDWWIIVPADKEIQTQPTGWDVYSFFSDGTQPGTGRDTIRGA
                     GAPLLAWTPPGSITFATAAASTAPTAGPIASAPPATSAPATAAPTPAASSSGTPTTSA
                     GGLSTIFLGLILLGLLLIFMGWWLFRRRGRPSSPTSPAPGEAPGSEAGASTDPGPTRP
                     ARPGPPASPPPGPGPTDPGPVAPGGPVVSDPGKPTPPIPPPPPPPPPRNCVEGDEEWR
                     NDKLATTFLIAPADARVRLTADPTTAALEAWMAPFGFPHGATAAGFGAIDDAALDALL
                     AGLPAGLSTFHWVFEFELDEYRLECERKWICRNDVWVSTDETRLQETGPTPNAARVAV
                     DGPARTRDDVRRAWLQVRGAMIGAATAEAAMDAYRKAC"
     gene            complement(3765..4367)
                     /locus_tag="VIF63_06675"
     CDS             complement(3765..4367)
                     /locus_tag="VIF63_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239101.1"
                     /translation="MGNLRIEPLTASSWDALAALFGEGGDPRWCWCQFQRLRSKDMSA
                     MKVPQLRERLRRQASSDEPPGLVAFDGDRAVGWVSLGPRTDFERIVRSRVIPKIDDRP
                     VWSIVCFAVSKSSRGAGVGRALLAGAIDYARGRGATALEAYPVRLETADDGTAEAIHP
                     DAAFTGTLPMFEHAGFRVVADRASDPSSSSQRVVVRRELP"
     gene            complement(4372..4812)
                     /locus_tag="VIF63_06680"
     CDS             complement(4372..4812)
                     /locus_tag="VIF63_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8239102.1"
                     /translation="MTAIGTMAPSAEIRETMLELYKRMLAGESDGANDLISRDPALVF
                     IGSAGEWIDDQAVLRSGTQVAGEGLEAGPNPVGYANGDVGWFADQPTWLFADGTRAEM
                     RLTSVLQHEAAGWRIVNVHMSVAVPDDQCVMLQRRWKYGIPATD"
     gene            complement(4809..5477)
                     /locus_tag="VIF63_06685"
     CDS             complement(4809..5477)
                     /locus_tag="VIF63_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8239103.1"
                     /translation="MADAGIYDGTRIVGYDDVGGWVAARLWWMLDSLGFGHRGNDGEW
                     AGVLDGGIKSWVEAGHELSTAMPAPPASPATLTLGGPWRGVIEREDLKARLGTLTLLD
                     ARAPERYRGEVEPVDPVAGHIPTAISAPFGANLDANGRMKDVAGLRARYAELDIVPSS
                     EGEVVVSCGSGTSAAHHSLAMRAAGLPDPILYVGSYSDWSRSGEEIVTGPEPGPPPNT
                     WREQ"
BASE COUNT          843 a         2009 c         1925 g          772 t
ORIGIN      
        1 gaccgcaagg gcgacatcgt ggtcatcggc cgcgacgccc gcgagcagct gacctcggcc
       61 aacctgcggc tggtcgtctc gatcgccaag aagtacatcg gccgcgggat gtcgttcctg
      121 gacctcatcc aggagggcaa catcggcctc atccgcgcgg tggagaagtt cgactacgag
      181 aagggcttca agttctcgac gtacgcgacg tggtggatcc gccaggccat caccagggcc
      241 atcgcggacc aggcgcggac catccgcatc ccggtccaca tggtggagac catcaaccgc
      301 ctgatccggg tctcgcgcgg cctgctccag gagctcggtc gcgagcccac ggtcgaggag
      361 atcgcggagg cgatgtcgcg cggccaggag gtcgtggtca cgcccgagaa ggttcgcgag
      421 atcatcaagg tctcgcagga acccgtgtct ttggagacgc cgatcggcga ggaggaggac
      481 tcccacctgg gtgacttcat cgaggatcgg ggcgccctcg ccccggccga ggccgcgtcg
      541 caccagctcc tcaaggagca ggtcgaggcc gtcctcgact cgctgaccgg ccgcgaacgg
      601 cgcgtgctcc agctccggtt cggcctcgaa gacggccgcg cccggacgct cgaggaggtc
      661 ggcaaggagt tcaacgtgac ccgcgaacgc atccgccaga tcgaggcgaa ggcgctccgc
      721 aagctccgcc acccgtcccg cagccgcaag ctcaaggact acctggagta gagggcaggg
      781 gagcggcggg tctcatcccg tcgctccctg ctcgttcgcg ccggggtccc accgggccgt
      841 tcgccgtcgt cgctcgccgc tcgcgcctcg ccccggcctc gatccttcat tgcatgttcg
      901 atctggcggg cgaacgcggt cgcggagccc atcggccacg gcattcgcct cgccccggcc
      961 tcgatccttc atcgaatgtt cgatctggcg ggcgaacgcg gccgcggagc ccttcctccg
     1021 tccgatcgtg catcgaatgg aggggatcga ggctgagggc ctcgatcccc gagcgtggcg
     1081 ggccccacga gggttttcgc ccgccgcaac gtgcatctga tgaaggcccg acccgcaatt
     1141 cgcgggcgaa tccggcctag agcacaacca acccgttgcc ggtgaccgcc tcgccccggc
     1201 ccaaggatcg aagggctgac cgggtcggga tcggaaacgt ctctcgtagt gccgggccgg
     1261 ctacctcgag gagcgtacgg tggtggcggg tatcgctcag gagcaggatg gctgtcgccg
     1321 catgtccgtc gcggagcttg agcgcgagcc ggcgctcgag ggcctggaca tcacgaacat
     1381 gtgtctcggc ctcgacgccg atcgtccaac cgggccccga gatcgtggcg tcccaggcac
     1441 gggagtccat tgatgccgtt tcgccctgcg cggtgaccgg gacttcggtg cgccaacgga
     1501 gggacggtcc gagacgagcc cgaagccgag cgagcagcgc gaggtgaccg gcgtcgcgaa
     1561 gcggcggccc agcgggaaaa cctcgggcgc tcagatcgag accgacgagt gcatagagct
     1621 gcgccatccg tgcgatcgag acgtcgggcg acctgccctg gcagatccgc gcaacctgca
     1681 cgccggacag gcccagcggg cgtccgagcg ccacgtagct gaggccgccg tcgagccgcg
     1741 cttcctcgag atccgcacat agcttcgtca cgagcgagcg ccctcgcgca acacctcgat
     1801 cggcactggc cactcgaacg ggcatcaact gagtttcatc gaccgggctt ggctggcggt
     1861 tacgcggcgg aggtccgcgg cgcaggaagg gctggattcg gagtcggcgt tcggctagat
     1921 tgcgcgcact cgggtcggtc gggagggggc cggatgggtc gcgtgatcgg ggagcggatc
     1981 ggggcattgc ggacgaggcg gccgcggcgc gacatcgcag cgcgcgcacg gcccgccgtc
     2041 gcagcgcggc gtgccatcgc agtccggcgc gtactcgcgg tgcttgccgc gggcgtcctt
     2101 gcgctcgggt ccatcgtggc acccgtctcg gccggcaccc cgccgggggc ggatccactc
     2161 ctcgaaatcc tcaaggtcta cgccgaggtc gaggggctcg acgtcgtcgt cgaaggcaac
     2221 atcgtccgcc tcgaggaccc cctgggcgat gtctccggca acgacgccgg cgggaacgac
     2281 atcgcgagtg ccggctacgc gtggctgccg atggtgcccg ccgggctgct cgacggcccg
     2341 ttcaaatgcg gcgaggcgaa ggtcgcgtgc gacaacgccg cgaccgatcg ctacgcccac
     2401 ggcgcctggt tgttccacgg tcggcgggca atgccgtacg gcgatgtgcc ggccggcgcg
     2461 cgtgtcgagt tcgggccgca gctcgcgttg atgcaatacc ccgccgcccc catcgagggc
     2521 gacaacccgt tcggcggcac gtcgcacaac gtgatcgcgc gcatccaggg cggcacgggc
     2581 gagatgcact acttcggcta cgacggcacc acgttcccgg agttcaacac ccagtcgcgg
     2641 gcaacgtgga ctgcgaccga ctggtggatc atcgtgcccg ccgacaagga gatccagacg
     2701 cagccgaccg gctgggacgt ctattcgttc ttcagcgacg gcacgcagcc cgggacgggg
     2761 cgcgacacga tccgcggcgc cggcgcaccg ctcctggcct ggacgccgcc ggggtcgatc
     2821 acgttcgcga ccgcagcggc ctcgaccgcg ccgaccgccg gcccgattgc gagcgcgccg
     2881 ccggcgacgt cggcgccggc aaccgcggcc ccaacacccg ccgcgtcgag ctccggcacg
     2941 ccgaccacgt ccgcgggcgg attgagcacg atcttcctgg ggctcatcct tctcgggctg
     3001 ctcctgatct tcatgggctg gtggctgttc cgccgtcgcg gccggccatc cagcccgaca
     3061 tcgccggcac cgggcgaagc acccggatcg gaagccggcg cgtcgaccga ccctggaccc
     3121 acgcgcccgg cgcgaccggg gccgcccgca agtccgccac ccggacccgg tccgaccgat
     3181 cccggcccag tcgcacccgg cggcccggtc gtgagcgacc ccggcaagcc gacgccgccg
     3241 atccctccac cgcccccacc gccaccgccg cgcaactgcg tcgagggcga cgaggagtgg
     3301 cgcaacgaca agctggcgac gacgttcctg atcgcgccgg ccgatgctcg cgtccggctc
     3361 accgccgacc cgacgacggc agcgctcgag gcgtggatgg cgcccttcgg cttcccgcac
     3421 ggtgcgaccg cggccggctt cggtgcgatc gacgacgctg cgctcgatgc gctcctcgcc
     3481 ggcctgccgg cgggcctgtc gacgttccac tgggtcttcg agttcgagct cgacgagtac
     3541 cggctcgaat gcgagcgcaa gtggatctgc cggaacgacg tctgggtctc gacagacgaa
     3601 acgcgcctcc aggagaccgg cccgacgccg aacgccgccc gcgtcgcggt cgatgggccg
     3661 gcccgcacgc gcgacgacgt ccggcgcgcc tggctgcagg tccgaggcgc gatgatcggc
     3721 gccgccacgg ccgaagcggc gatggacgcg taccgaaagg cctgctaggg cagctcccgg
     3781 cgcaccacga cccgctggct ggagctcgac ggatccgagg cgcgatccgc cacgacccgg
     3841 aaacccgcgt gctcgaacat cggcagggtg ccggtgaacg ccgcgtccgg atggatcgcc
     3901 tcggccgtgc cgtcgtcggc cgtctcgagc cggaccgggt aggcctcgag ggccgtggca
     3961 ccgcggccgc gggcgtagtc gatcgcgccg gcgaggagtg cgcgacccac acccgcacca
     4021 cgagacgatt tcgacaccgc gaagcacacg atcgaccaca ccggtcgatc gtcgatcttc
     4081 gggatgaccc gcgatcggac gatgcgctcg aaatcggtcc tcggcccgag gctgacccag
     4141 cccaccgctc gatcgccatc gaacgcgacg aggcccggcg gctcatcgga gctcgcttgc
     4201 cgccgcaatc gctcacgaag ctgcggcacc ttcatcgcgg acatgtcctt cgatcgcagg
     4261 cgctggaact ggcaccagca ccagcgcgga tcgccgcctt ccccgaacag ggcggcaagc
     4321 gcgtcccagc tcgacgcggt cagcggttcg atcctgaggt tgcccacggg gtcagtccgt
     4381 cgcggggatc ccgtacttcc aacggcgctg gagcatcacg cactggtcgt ccggcaccgc
     4441 gacggacatg tgcacgttca cgatccgcca gcccgcggcc tcgtgctgga gcaccgacgt
     4501 gagccgcatc tcggcgcggg tgccatcggc gaagagccag gttggctggt cggcgaacca
     4561 gccgacgtcg ccgttggcgt agccgaccgg gttcgggccg gcctcgaggc cctcgcccgc
     4621 gacctgggtt cccgagcgga gtacggcctg gtcgtcgatc cactcaccgg ccgagccgat
     4681 gaacacgagc gccgggtcgc gtgagatgag gtcgttcgcc ccgtccgact cgccggcgag
     4741 cattcgcttg tacagctcga gcatcgtctc gcggatctcc gccgacggcg ccatcgtccc
     4801 gatcgcggtc attgctccct ccacgtgttg ggcggcggcc ccggctcggg tcccgtgacg
     4861 atctcctcgc ccgatcggct ccagtcggag tacgagccga cgtacaggat cgggtcaggc
     4921 aggcccgccg cgcgcatcgc aagcgagtgg tgcgcggcgg acgtcccgct cccgcacgac
     4981 acgacaacct cgccttcgga cgacggcacg atatcgagct ccgcgtaacg cgcacggaga
     5041 ccggcgacgt ccttcatgcg gccattcgca tcgaggttcg caccgaacgg tgcgctgatc
     5101 gcggtcggga tgtggccggc caccggatcg accggctcga cctcgccgcg gtagcgctcg
     5161 ggcgcgcgcg catccaggag ggtgagcgtg ccgaggcgag ccttgaggtc ctcccgctcg
     5221 atcacgccgc gccacgggcc gccgagcgtc agcgtggccg gcgaagcggg cggagcaggc
     5281 atggctgtcg agagctcgtg gcccgcctcg acccacgact tgatcccgcc gtccaggacg
     5341 cccgcccatt cgccgtcgtt gcctctgtgt ccgaagccga ggctgtcgag catccaccac
     5401 aggcgcgcgg ccacccagcc gccgacgtcg tcgtagccca cgatgcgcgt tccgtcatag
     5461 attccggcgt cagccatgcg cgcggcgaag gccgccggat cggggagggg atgccggcct
     5521 ggggccccgt atccgtgatg gtcggcgag
//
LOCUS       DATBSW010000241         6547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_763172, whole genome shotgun sequence.
ACCESSION   DATBSW010000241 DATBSW010000000
VERSION     DATBSW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763172"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VIF63_06690"
     CDS             complement(<1..1043)
                     /locus_tag="VIF63_06690"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY8239104.1"
                     /translation="MRILVIGAGGVGSAVVPTAIRRDFFEHLVVADYDAGRANAVAAR
                     AADPRVTAAQVDASNRESIAALIREHGATHVLNAVDPRFVMPIFDACLAAGADYMDMA
                     MSLSKPHPERPYELSGVKLGDEQFAAAGKWESAGRLALVGMGVEPGLSDVFARYAQDH
                     LFSEIDECGVRDGANLTVDGYDFAPSFSIWTTIEECLNPPVIWERGRDWFTTEPFSEP
                     EVFEFPEGIGPVECVNVEHEEVLLIPRWVKARRVTFKYGLGNEFIEVLKTLHKLGLDR
                     TAKVRVGGVEVSPRDVVAAVLPDPATLGDKMHGKTCAGTWVTGTGKDGKPREVYLYHV
                     VDNAWSMAEYGSQA"
     gene            1080..1886
                     /locus_tag="VIF63_06695"
     CDS             1080..1886
                     /locus_tag="VIF63_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8239105.1"
                     /translation="MTDALRVERTGPGGVVARVTLTRPKVRNAFDASLIAELRTTFAA
                     LERESVIDLRVVVLAGEGETFCAGADIDWMRAAMQLDVEGNEQDSMAMADMFETIDTC
                     PVPVIARVQGAALGGGMGLCAVSDLVIAESGAKFGFTETRLGILPAVISPFVIAKIGE
                     SHTRALFPGGRRFDALRAQRIGLVHEVVEGDEALDAAVESAIGDILASGPTAVRAAKA
                     IVREVRGLGHGSSKWHTARVIARQRVSAEAKEGFAAFSEKRPAGWVPPED"
     gene            complement(1843..3138)
                     /locus_tag="VIF63_06700"
     CDS             complement(1843..3138)
                     /locus_tag="VIF63_06700"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239106.1"
                     /translation="MLGSLGRYLGLLRERDFRRLWIAQSVSQVGSEVTLLALPLVAVL
                     VLDASPFEVALLGATQFFAFILVALPAGAWVDRLPRTRILVGADLGRAAILVVVPLAA
                     ATHFLSMGLLYTVIFGTSILRVFFDIGYQAILPDLIERHRLAEGNSRLEVSSSAAQLL
                     GPGIGGWLVGLLSAPIAIVVDALSFLGSAAFLVGIRARPVASPGGPARPRSGMAREIL
                     EGISFYRRNPLLRSLAATSVIYYGGVWFAGGTFIAYLARDLQMSPEAIGLGYSIGAIG
                     VLAGAATAAAMGRRFGIGPTLIVSIAIGALSWAIMAFARPETALAFLALSAIGSGITS
                     MAMSINYVSLRQAITPPELQGRVNATGRWLNWTVIPLAAIGGGAVATAVGIRETILIG
                     SGVAFLAVPILFLSPLGSLRAVPGAISPPEEPNPPAASR"
     gene            3372..5273
                     /locus_tag="VIF63_06705"
     CDS             3372..5273
                     /locus_tag="VIF63_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8239107.1"
                     /translation="MDPEQPPSAIHPQASRTARGFPEQPAARRSIARLFIANRGEIAN
                     RIRRTTDRLGIVAIEPRTVGHGAVDLLDIDAVVAAARTAGTDAIHPGFGFLAENAAFA
                     QAVEAAGIAWIGPPPEAIRAMGDKAAARRLATKLGVPTVPGYDGADQSDAALTRAARR
                     IGYPLLVKPAAGGGGKGMRTVREPARLADALSSARREAVTAFDDDRLILERLVEGPRH
                     VEVQVLFDAPGSGIHLGERDCSTQRRHQKVFEETPSPAVTAALRERLTAYALQLAAAV
                     GYRSAGTCEFLLTDRGEVFFLEMNTRLQVEHPVTEAVTGLDLIEQQIRIADGRPLEQT
                     QAAIDEALTTGGHAIEVRLYAEDAEDGFLPATGKVERLTWPSGTGVRVDAGIEEGTMV
                     GSRFDPMLAKVIAHGADRAEAMARLTRALDDTVVLGLTTNLRFLRWLVRQPVVLEGQM
                     RIDTLDRIWPPDDWAARSTVPELAWQAAARALAPDGWLGGWRLNGPPRIRVATDGAER
                     IVAIDAAAPRARAVVVAGEVAHVDVAGRSTPFRLAPAPDVDRSARAAAASHGVGPVDI
                     IAPMPGSILAVHASVGQPIEAGDRIATLEAMKMEHAVVAAAPGRIAELRAKPGDQVAR
                     GDVLAVVEP"
     gene            5310..5528
                     /locus_tag="VIF63_06710"
     CDS             5310..5528
                     /locus_tag="VIF63_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239108.1"
                     /translation="MTTKAAPKPNAKADSKPLPEDRASLLALHREARRRRDAAPLLSH
                     ARAEAAFEIERIEIQVARIERAMDPPLV"
     gene            5549..6475
                     /locus_tag="VIF63_06715"
     CDS             5549..6475
                     /locus_tag="VIF63_06715"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY8239109.1"
                     /translation="MGDRVRIYEVGPRDGLQNESTAIPLATKLAFIERLVTAGLREIE
                     ATSFVSPKAIPQLADADELVPELRGAGVRFPVLVPNERGMARAEAAGADALAVFTAAT
                     DAFTEHNIGMTVDESLAAFEPVLQRATDLGWWKRAYVSTAFGCPYTGAVDPQRVVVVA
                     TRLLDLGVDEVCFGDTIGVGVPSQVAVLTGAAVAAGIALDQIAYHFHDTRGTALANVQ
                     AGLDAGVRCFDSSTGGTGGCPYAPGAAGNLATEDLVYLLDASGFEHGVSLEGILDAAR
                     FITGALGKPLTSKVGQAGGWDATTGAATGRLA"
BASE COUNT         1018 a         2241 c         2363 g          925 t
ORIGIN      
        1 gcctgcgagc cgtactcggc catcgaccag gcgttgtcga ccacgtggta caggtagacc
       61 tcgcgcggct tgccgtcctt gccggtcccg gtgacccacg tgccggcaca cgtcttgccg
      121 tgcatcttgt cgccgagcgt cgccggatcc gggagcacgg ccgccaccac gtcgcgcggc
      181 gagacctcga cgccaccgac ccggaccttg gcggtccggt cgaggccgag cttgtgcaaa
      241 gtcttgagga cctcgatgaa ctcgttgccg aggccgtact tgaacgtcac gcggcgggcc
      301 ttcacccagc gcgggatcag caggacctcc tcgtgctcga cgttgacgca ttcgaccggc
      361 ccgattccct ccgggaattc gaacacctcg ggctcgctga atggctcggt cgtgaaccag
      421 tcgcggccgc gctcccagat caccggcggg ttgagacact cctcgatcgt cgtccagatc
      481 gagaacgacg gcgcgaagtc gtagccgtcg acggtcaggt tggcgccgtc gcggacgccg
      541 cactcgtcga tctcgctgaa gaggtggtcc tgggcgtagc gtgcgaagac atctgacagc
      601 cccggctcca cgcccatgcc aaccagcgca agccgcccgg cggactccca cttcccggcg
      661 gccgcgaact gctcgtcgcc gagcttgacg ccggagagct cgtaggggcg ctccgggtgc
      721 ggcttcgaaa gcgacatggc catgtccatg tagtcggcgc ccgcggcaag acaggcatcg
      781 aagatgggca tgacgaagcg cgggtcgacg gcgttcagga cgtgcgtcgc gccgtgctcg
      841 cggatgagcg ctgcgatcga ctcgcgattc gaggcatcga cctgcgctgc ggtcacccga
      901 gggtctgccg ctcgcgcggc gacggcgttg gcgcgtcctg cgtcgtagtc ggcgacgacg
      961 agatgctcga agaagtcacg gcggatcgcg gtcgggacga ccgcgctgcc gacgccgccg
     1021 gcgccgatca cgaggattcg catcggcgca gcgtacccgg gccgcgtagg atgccgcccg
     1081 tgaccgacgc gctgcgagtc gagcggaccg ggcctggtgg tgtcgtggcg cgggtgacgc
     1141 tgacccgccc gaaggtccgc aacgccttcg atgcgtcgct catcgccgag ctgaggacga
     1201 catttgcggc gctcgagcgg gaatcggtga tcgacctgcg cgtggtggtc ctcgccggcg
     1261 aaggcgagac gttctgcgcc ggcgctgaca ttgactggat gcgcgcggca atgcagctcg
     1321 acgttgaggg caacgagcag gactcgatgg cgatggcgga catgttcgag acgatcgaca
     1381 cgtgcccggt gccggtgatc gcgcgagtcc agggggcggc gctcggcggc ggcatggggc
     1441 tgtgcgccgt ttcggacctg gtcattgccg aatccggcgc caagttcggg ttcaccgaga
     1501 cgcggctcgg gatcctgccg gcggtgatct cgccgttcgt gatcgcgaag atcggcgaat
     1561 cgcacacccg agccctgttt cccggcggcc gtcgcttcga tgccctgcgg gcgcagcgaa
     1621 tcgggctggt gcacgaggtg gtcgagggcg acgaagcgct cgacgccgcg gtcgagagcg
     1681 ccatcggcga catcctcgcg tccggcccga ccgcggttcg cgccgcgaag gcgatcgtgc
     1741 gtgaggtgcg cggcctcgga cacggctcgt cgaagtggca cacggcgagg gtgatcgccc
     1801 gccagcgagt gagcgcggag gcgaaggaag ggttcgcggc gttcagcgag aagcggccgg
     1861 cgggttgggt tcctccggag gactgatggc acctggcacc gcgcgcagcg agccgagcgg
     1921 tgacagaaac aggatcggca ccgcgaggaa tgcgaccccc gagccgatga ggatcgtctc
     1981 ccggatcccg acggcggtgg caaccgcacc gccaccgatg gccgcgagcg ggatcaccgt
     2041 ccagttgagc cagcgacccg tggcattgac ccggccctgg agctcgggcg gcgtgatcgc
     2101 ctggcgaagg ctgacgtagt tgatgctcat ggccatcgag gtgatgccgc tgccgatcgc
     2161 gctgagcgcc aggaatgcca gcgccgtttc gggccgagcg aaggccatga tcgcccacga
     2221 cagggcgccg atcgcgatcg acacgatgag cgtcggtccg atgccgaagc gacggcccat
     2281 cgcggccgcg gtggccgctc ccgcgaggac gccgatcgcg ccgatcgaat agccgaggcc
     2341 gatcgcctcc ggcgacatct ggaggtcgcg agcgaggtag gcgatgaacg tgccgcccgc
     2401 gaaccagacg cccccgtagt agatgacgct cgtcgcggcg agcgagcgca gcagcgggtt
     2461 gcggcggtag aacgagattc cctcgaggat ctccctcgcc atccccgacc gcgggcgggc
     2521 cgggccaccc gggctcgcga cgggtcgagc ccgaatgccg acgaggaagg ctgccgaacc
     2581 gaggaagctg agggcgtcga cgacgatcgc gatcggcgcg ctgagcaggc cgacgagcca
     2641 gccgccgatg cccggaccga gcagctgcgc cgccgagctg ctgacttcga gccgtgagtt
     2701 gccctccgcg aggcggtggc gttcgatgag gtccggcagg atcgcttgat agccgatgtc
     2761 gaagaagacc cgcaggatcg acgtgccgaa gatcaccgtg tagaggaggc ccatcgacaa
     2821 aaagtgggtc gcagcggcga gtggaaccac gacgaggatc gcggcccggc ccaggtcggc
     2881 gccaacgagg atccgggtcc ggggcagtcg atcgacccag gcgccggctg ggagcgcgac
     2941 caggatgaac gcgaagaact gcgtcgcacc gaggagcgcg acctcgaacg ggctggcatc
     3001 gagcacgagc actgcgacca gcggcagcgc gagcagcgtc acctcgctgc cgacctggct
     3061 caccgactgg gcgatccaga gtcgtcggaa gtcacgctcg cgaagcaggc cgaggtagcg
     3121 gccgagggat ccaagcacgg ggacgaagga tattgcgcgc gacgaggcat ggattcccct
     3181 cgtccgcttg ccggcgatgc cgatagacgc gacaatcacg ccacatgcgc gaggagatga
     3241 gcccgagcga ggcagcgcgc cgccttggca caacgacgcg gtccgtccaa cggtggattg
     3301 cgctgggcag gctgcccgca cgacgtgttg ggggtcgctg gcgtgtcgcg tttgacgcgt
     3361 tagacgcgtt cgtggatccc gagcagccac cgtcggcgat ccacccacag gcatcgcgga
     3421 ccgcacgagg attccccgag cagccggccg cccgccgttc gattgcgcgt ctgttcatcg
     3481 cgaaccgcgg cgagatcgcc aatcgcatcc ggcgcacgac cgatcgactc ggcatcgtcg
     3541 cgatcgagcc gcggacggtt ggtcacgggg cggtcgatct cctcgacatc gatgcggtcg
     3601 tcgcggccgc gcgcaccgcc ggcaccgatg ccatccatcc cggattcggc ttcctggccg
     3661 agaacgccgc attcgcccag gcagtcgagg ctgcggggat cgcctggatc gggccgccgc
     3721 ccgaggcgat tcgcgcgatg ggcgacaagg cggccgcgcg ccgccttgcc acgaagctcg
     3781 gcgtgccgac cgtgcccggc tacgacggcg cggaccagtc ggacgccgcg ttgacaaggg
     3841 cggcgaggcg catcggctac ccgctgctgg tcaagcccgc ggccggtggt ggtggcaagg
     3901 gcatgcgcac cgttcgcgag ccggcacggc tggcggacgc actctcgagc gcgcgccgcg
     3961 aggcggtcac ggcgttcgac gacgaccggc tgatcctcga gcggctcgtc gagggtccgc
     4021 ggcacgtcga ggtgcaggtc ctgttcgacg ccccggggtc cggcatccac cttggcgagc
     4081 gcgattgctc gacccagcga cgacaccaga aggtgttcga ggagaccccg tcaccggcgg
     4141 tcaccgcggc ccttcgcgag cgcctcacgg cgtatgcgct gcagctcgcg gccgcggtcg
     4201 gatatcggag tgcggggaca tgtgagttcc tcctgaccga tcgcggtgag gtgttcttcc
     4261 tggagatgaa cacccgactg caggtcgagc atcccgtgac cgaggcggtg accggcctgg
     4321 acctcatcga acagcagatc cggatcgccg acggccggcc actcgagcag acgcaggccg
     4381 cgatcgacga ggcgctgacg accggcggtc acgcgatcga ggtccgtctc tacgccgagg
     4441 acgccgagga cggctttctg ccggcgaccg gcaaggtcga gcggctgacc tggccgtcgg
     4501 gcacgggcgt tcgcgtcgat gcggggatcg aagaaggcac gatggtcggg tcgcgcttcg
     4561 atccgatgct cgccaaggtc atcgcgcacg gcgcagaccg cgccgaggcc atggcacgac
     4621 tgacccgtgc cctcgacgac accgtcgtgc tcgggctcac gaccaacctg cgcttcctcc
     4681 ggtggctggt gcgccagccg gtcgtcctcg agggacagat gcggatcgac accctcgatc
     4741 ggatctggcc gccggacgac tgggcggctc gctcgaccgt tccagagctg gcgtggcagg
     4801 cagcagcacg agccctggcg ccggatggtt ggctcggtgg ctggcgcctc aacgggcccc
     4861 cgcgcatccg ggttgcgacc gatggcgccg aacgcatcgt cgcgatcgat gccgcggcgc
     4921 cccgcgcgag ggccgtcgtg gtcgcgggcg aggtcgcaca cgtggacgtc gcgggacgga
     4981 gcacgccgtt ccggctcgcg cccgcgcccg atgtcgatcg gtcggcgcga gctgcggcgg
     5041 cgtcgcacgg cgtcgggccg gtcgacatca tcgcgcccat gcccggctcg atcctggcgg
     5101 tgcacgcttc agtcgggcag ccgatcgagg ccggcgatcg gatcgcgacg ctcgaggcga
     5161 tgaagatgga gcacgcggtc gtcgcggctg ctcccgggcg aatcgccgag ctgcgggcga
     5221 agcccggcga ccaggtggca cgcggcgacg tcctcgccgt cgtcgaaccg tagccaactc
     5281 tcctgcgtgg ctcgcggtac gctctcgccg tgaccacgaa ggccgcgcca aagccgaacg
     5341 cgaaggcgga ctcgaagccg ttgcccgagg atcgagcgtc cctgctcgcg ctgcaccgcg
     5401 aggcacgccg gcggcgtgac gcggcgccgc tgctgagcca cgcgcgcgcc gaggccgcgt
     5461 tcgagatcga acggatcgag atccaggtcg cccgcatcga gcgggcgatg gacccgccgc
     5521 tcgtctgacg gtcgcgtccc aaccgcgcat gggcgatcgc gttcggatct acgaggtcgg
     5581 cccgcgcgac gggctccaga acgagtcgac cgcgatcccg ctcgcaacca agctcgcgtt
     5641 catcgagcgg ctcgtgaccg ccgggctgcg cgagatcgag gcaacgagct tcgtgtcgcc
     5701 caaggcgatc ccgcagctgg ccgacgccga cgagctcgtc ccggagctgc gcggagccgg
     5761 ggttcggttc ccggtcctcg ttccgaacga gcggggcatg gcccgcgccg aggcggccgg
     5821 cgccgatgcg ctcgccgtgt tcaccgcggc caccgacgcc ttcacggagc acaacatcgg
     5881 catgaccgtc gacgagtcgc tcgcggcctt cgagcccgtc ctccaacggg cgaccgatct
     5941 cggttggtgg aagcgcgcct atgtgtcgac cgccttcggc tgcccctaca cgggcgccgt
     6001 cgatccgcag cgcgtcgtcg tggtcgcgac gcggcttctg gacctcggcg tcgacgaagt
     6061 ctgtttcggc gacacgatcg gcgtcggcgt gccgagccag gttgccgtgc tgaccggcgc
     6121 cgcggtcgcc gccgggatcg cgctcgatca gatcgcgtac cacttccacg acacgcgcgg
     6181 caccgccctc gcgaacgtcc aggccgggct cgacgctggg gttcgctgct tcgacagctc
     6241 gaccggcggc acgggtggtt gtccctacgc gcccggcgct gccggcaacc tcgccaccga
     6301 ggacctggtc tacctgctcg acgcctcggg cttcgagcac ggcgtctcgc tcgaggggat
     6361 cctcgacgcc gccaggttca tcaccggcgc cctgggcaag cccctcacgt cgaaggtcgg
     6421 ccaggccggc ggctgggacg caaccactgg tgcggcgacc ggccgcttgg catagcatcc
     6481 gcgcacgatg aacggggtgg tgctggtcct gaaccaggat tacgagccgc tgaatgtgac
     6541 gaacctt
//
LOCUS       DATBSW010000242         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_58159, whole genome shotgun sequence.
ACCESSION   DATBSW010000242 DATBSW010000000
VERSION     DATBSW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58159"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VIF63_06720"
     CDS             complement(<1..334)
                     /locus_tag="VIF63_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY8239110.1"
                     /translation="MTQVMVVVASRHGATRGIADKIGETLRAEGLEASVYDANEAPSP
                     QSADAVVVGGAAYMGKWLDEVADYVRRHQELLAHRPTWLFTSGPIGKDAVDQKGRDLV
                     ASPAFLTDA"
     gene            complement(432..833)
                     /locus_tag="VIF63_06725"
     CDS             complement(432..833)
                     /locus_tag="VIF63_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8239111.1"
                     /translation="MKRILVAYDGGEPAKRALEEAALLAKTFDALISVVSVVPSRPGR
                     SPMDPWDDDAVHARELEEARSLLALQGIEADLLEPIGDPARTIERIARDGGFDVVVVG
                     SRGLGAVSRFLQGSVSEHVATHTEATVVIVR"
     gene            complement(830..1315)
                     /locus_tag="VIF63_06730"
     CDS             complement(830..1315)
                     /locus_tag="VIF63_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8239112.1"
                     /translation="MTALMSPDVIEGQVVDREHRHLRAGLSNLREAIGRSHAMSRSDA
                     IDRTVRTLAWLRREVLPHAAWEEAWLYPHLDAAAATPWATRALRFQHDQIKEVATALE
                     REFASAEVRWSAEEAFQLAVALARLETLISAHLAQEQWFVGPLLEMTEPAGMTPNGGR
                     S"
     gene            complement(1464..>1751)
                     /locus_tag="VIF63_06735"
     CDS             complement(1464..>1751)
                     /locus_tag="VIF63_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239113.1"
                     /translation="VARRDAGHRAIVGIERDADPVHDYLLRVARAAEDCDRVMILGPG
                     DDRLEFEHEYEILYRRGDRLVDVEVRPATTNAELIDRLRLLEGDELARPAG"
BASE COUNT          245 a          628 c          597 g          281 t
ORIGIN      
        1 ccgcgtcggt caggaaggca ggcgaggcga cgaggtcccg acccttctgg tcgaccgcat
       61 ccttcccgat cggaccgctg gtgaacagcc acgtcggccg gtgcgcgagc agctcctggt
      121 ggcgccggac gtagtcggcg acttcgtcga gccacttgcc catgtaggcc gcgcccccga
      181 cgaccaccgc gtcggccgac tgggggctcg gtgcctcgtt cgcgtcgtac acgctggcct
      241 cgagcccttc cgcgcggagg gtctcaccga tcttgtccgc gatgcctcgc gtcgcaccat
      301 ggcggctggc gacgacaacc atcacctgtg tcatcaggat cctcctcttc tcaatcccga
      361 ggcccgccgg tatcgccggc gggcctcgta ccccggtgca gccttctgct gaacgctccg
      421 cgcacgccga atcagcgaac gatgaccacc gtcgcctcgg tatgggtggc aacgtgctcg
      481 gagacgctgc cctggaggaa ccgcgagacc gcgcccaggc cacgcgaccc gaccacgacg
      541 acgtcgaaac cgccatcgcg ggcgatccgc tcgatggtgc gtgctgggtc gccgatcggc
      601 tcgagcaggt cggcctcgat cccctgcagg gcgaggagcg agcgcgcctc ctcgagctcg
      661 cgtgcgtgca ccgcgtcgtc gtcccacgga tccatcggcg aacgccccgg gcgcgacggg
      721 accacgctga cgacgctgat gagggcgtcg aatgtcttgg cgagcagcgc ggcctcctcg
      781 agggcacgct tggcgggctc gcccccgtca taggcgacga ggatccgttt catgatcgac
      841 ctccattcgg tgtcatgccg gccggctcgg tcatctcgag cagcggcccg acgaaccatt
      901 gctcctgggc gagatgcgcg gagatgaggg tctcgagccg ggccagcgcg accgccagct
      961 ggaatgcctc ctcggcgctc cagcgaacct cggccgaggc gaactcgcgc tccagtgccg
     1021 ttgccacttc cttgatctgg tcgtgctgga agcgcagtgc ccgggtcgcc cacggcgtcg
     1081 ccgctgcggc atcgaggtgc gggtacagcc aggcctcctc ccaggccgcg tggggcagga
     1141 cctcacgcct gagccaggcg agcgtccgga ccgttcggtc gatcgcgtcc gagcgcgaca
     1201 tggcgtgtga tcggccgatc gcctcccgga gattcgacag tccggcacgg aggtgccggt
     1261 gctcacgatc gacgacctgt ccctcgatca cgtcgggcga catcagggcc gtcatcgcac
     1321 acctcctggc gagcaactag cgggcggctc gcccgtttct cgccggattg atcgtcggcc
     1381 tgccgtgcgt cgccgggtag tgccaccggt accgtggtat gtcgccgttg gtcgtgatcg
     1441 cgcgtcgcag cggctcgaga gcgtcagccc gcgggccgag ccagctcgtc gccctcgagg
     1501 aggcggagcc ggtcgatcag ctcggcgttg gtcgttgccg ggcgcacctc gacgtcgacg
     1561 agtcgatcac ccctgcggta caggatctcg tattcgtgct cgaattccag ccggtcgtcc
     1621 cccggcccga ggatcatgac ccggtcgcag tcctcggccg cgcgagccac gcgcagcagg
     1681 tagtcgtgga cggggtccgc atcgcgttcg atgccgacga tggcccggtg accggcgtcg
     1741 cgccgggcga c
//
LOCUS       DATBSW010000243         6867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_23094, whole genome shotgun sequence.
ACCESSION   DATBSW010000243 DATBSW010000000
VERSION     DATBSW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23094"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3986
                     /locus_tag="VIF63_06740"
     CDS             <1..3986
                     /locus_tag="VIF63_06740"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8239114.1"
                     /translation="NGCGPTGKAVFDAAQIADPEIDYSDYDTDKDGVVDFFMMIFVGV
                     GGHGASQTSVPPYDNIWPHSSSLEFGYTNQGPYGQDGYVSDDQLKDLEGKPLFYTTSA
                     ALAMTTSVTAFPVYVRVGPYNVNPEDAVDHASVISHEYGHSLGLPDYYSIGRGTYGDW
                     NLMATDKSQHMDVNAKQELGWLIPRVLGPGQTDILNWTDTKANTHRIDWQQPDGTAYA
                     LSGTSVNNGQAYVAKLPARTILDESKFDAAGPGVGASREHAWWSQSGNDFGCAPKGGH
                     NLDVYLPELADVPPGTTVTVNFRSYWDMEWDYDYGFVLMSTDGGTTYESMESANGYTA
                     PAALNPQNNACQSKYGNGITGASGSFEAGTQNVDRLTSGYPDGGFLADSYDLSAAAGS
                     ATVLRFSYATDPGLARPGWFIDDLTVTAGSQVIYSTDLESGGNADDPRVFNGGCKEST
                     AVAAQCTDGWQFVDASAGSPADHAYYLEMRDRSGFDMDGKGENDREPIAFLPGLLLTY
                     TDEAHGYGNAGTPDPPAQSPLDSQPQAGNNTPNLNDAAWTAAAGDNHFSDSGAGWTDN
                     YKDPSSADGFWHFTWDCLTFDVLNMTGTNIGPSVVPPYNLSGDIRFTMGNGCARFDYG
                     HGGPGGPVNQPPTAVAEARPTTVGTGEPVTFDASGSFDDQQAPNELTYAWDLDDNGTY
                     ETPGQTVTRSFAVAGTYDVSVRVTDAGGLSNADTVTVTVEGVEACPPFTPTGSSSADF
                     ESGPAGWTPDTAQALPASVNWQVAPDPLAHSPNSSFFSDAVGVDLKDDRLVSPPMNLS
                     SQSQLRFWHRFGFEAEFDGGVLEVSTDDGSTWVDVLAGGGSFAEGGYNSAISAAYGSP
                     IAGRSAWTGPQEGATNPMTRVVVNLGAFAGDDVRVRFRLATDPFGAGALPGVGWWIDD
                     VSFTKLLEPATCDNHAPVAVDDAATTTTGTPVTVNVLGNDADPDADALSVDDVGSPAH
                     GTVTDNGDGTVTYTPNAGFTGSDRFTYDIVDGRGGSDTGTVSITVSSAGNRPPVAADD
                     SATTVEDDSVSIPVLDNDTDPDHDTLMVSDFSDPANGSVAVDGTGLRYTPDAGFVGTD
                     TFTYDAADGRGGFDTATVTVTVTEAPNGDPNAVNDVASTTRDTSVRIVVVANDTDPDG
                     DNLSVTGATNPPHGTTVNHGDGTVTYTPDSGFTGTDTFEYTISDGHGGSDTATVTLFV
                     NASSDTAKVNGGGWFTTGGSRSSVNVNAQVKNGLAKGKVGYDLDGGISIKGNVDALRI
                     TGSAADVTGSCTRANGAACTFEVHVVDNAEPGAGWDTFSIRVYDAAGALIHEASGILE
                     GGNLQVH"
     gene            4144..5838
                     /locus_tag="VIF63_06745"
     CDS             4144..5838
                     /locus_tag="VIF63_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HEY8239115.1"
                     /translation="MRRRGRLARFLASLALFGLGFSLGAQTAIAVNPELTLRPAKGAA
                     GSTFAATYEDMVPAGALCTVGTVDFWWDAAGPGSGKAGAKKLGSATIAVQDCVAVAPA
                     LKVPKASCGRHVVYAFRSRNGDPVPTSVTSAAFTVNGCATASPAPTARPTPAPTPSAP
                     PLPTATPGAEPSTAPSALPSGSPAASNPAAPTPASVGPLVTPPPVPPPPPPPSSVDLA
                     GILVLVILGLLPIGVWAAAVHVKLGIVSGGASKWAGLVLAVILGGGLTWAGSLKFGLK
                     PVPVSAEFEYTGGSQFWVVPEGVEVAHVWVMGAAGGAGCPLEPLDFQGHPHNAGGSGH
                     EITSSMPVTPGQVIQVNVGGKGHDGECDGSFGEASNALGGFNGGGAGGAGGKGIPSLV
                     SYNGGGGGGASDVRHAPYGLAARDVVAGGGGGGGGAASSDDSMKGGPGGCPTGEAGPG
                     ANGQGGGGGILTMGGIGGANGGNGGLSGEWGTFGLGGAGADRGSGQTAGGGGGGGWNG
                     GGGGGSGAGSQWGGGGGGGGCRWPEGGDYDDTNAVSFGNGRVVIQYTQYVSIFWGL"
     gene            complement(5840..6433)
                     /gene="ruvA"
                     /locus_tag="VIF63_06750"
     CDS             complement(5840..6433)
                     /gene="ruvA"
                     /locus_tag="VIF63_06750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8239116.1"
                     /translation="MIASLDGIVAAVALDSVIVEVGGVGYRVYVSPSVLASAPPGGRL
                     KLFTHHVVREDLQALYGFRGLEELGFFGLLLTVTGVGPKVAMAIVGSRPTAELQLAIM
                     QQDQAVLVSIPGIGKKLAERVIFELKEKVAAAGVAAIAVAGSPAGGEGDVVGALQALG
                     YSLPEAREASRVAIGMAGVDASLEERVKAALRMLARD"
     gene            complement(6430..>6867)
                     /locus_tag="VIF63_06755"
     CDS             complement(6430..>6867)
                     /locus_tag="VIF63_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8239117.1"
                     /translation="KPDLMAVERLFFSRNVQTAFAVGQARGVVLLAAAQAEVPVREAT
                     PAEVKLAVTGSGRADKEQVGRMVAVCLSLPAAPKQDDTSDALAIAIWAANAERPGSVE
                     RRSAVLDRASVNPMVATGAGGTSYERAVREALALEKTPARPKR"
BASE COUNT         1092 a         2494 c         2324 g          957 t
ORIGIN      
        1 acaacggctg cggcccgacc ggcaaggccg tgttcgacgc cgcccagatc gccgacccgg
       61 agatcgatta cagcgactac gacaccgaca aggacggcgt cgtcgacttc ttcatgatga
      121 tcttcgtcgg cgtcggcggg cacggcgcgt cgcagaccag cgtgcccccg tacgacaaca
      181 tctggcccca ttcctcgagc ctcgagttcg gctacacgaa ccagggcccg tacggccagg
      241 acggctacgt ctcggacgac cagctcaagg acctcgaggg caagccgctc ttctacacga
      301 cctccgccgc gctcgcgatg accacttccg tgacggcctt cccggtctac gtccgggtcg
      361 ggccgtacaa cgtcaacccc gaggacgcgg tcgaccacgc gagcgtgatc tcgcatgagt
      421 acggccactc gctcggcctg cccgactact actcgatcgg gcggggcacg tacggcgact
      481 ggaacctgat ggcgaccgac aagtcgcagc acatggatgt caacgccaag caggagctcg
      541 gctggctcat cccgcgcgtg ctcggcccgg gccagacgga catcctcaac tggaccgaca
      601 ccaaggcgaa cacgcaccgc atcgactggc agcagccgga cggcacggcg tacgcactgt
      661 ccggaacgag cgtgaacaac ggccaggcgt atgtcgccaa gctgcccgcc cgcaccatcc
      721 tcgatgagtc caagttcgac gcggcgggcc ccggcgtcgg ggcgtcgcgc gagcacgcct
      781 ggtggtcgca gtccggcaat gacttcggct gcgcgcccaa gggtggtcac aacctcgacg
      841 tctacctgcc cgagcttgcc gacgtgccgc ccgggaccac cgtcaccgtc aacttcaggt
      901 cctactggga catggagtgg gattacgact acggcttcgt gctgatgtcg accgacggcg
      961 gcacgaccta tgagtcgatg gaatcggcca acggctacac ggcgccggcg gcgctcaacc
     1021 cccagaacaa cgcctgccag agcaagtacg gcaacggcat caccggtgcc agcggctcgt
     1081 tcgaggccgg cacccagaac gtcgatcgcc tcacgtccgg ctacccggac ggcggcttcc
     1141 tcgccgattc gtatgacctg agcgcggccg ccggctccgc cacggtcctg cgcttctcgt
     1201 atgccacgga tccgggcctc gcccggccgg gctggttcat cgacgacctc acggtcaccg
     1261 ccggctcaca ggtcatctac tcgaccgatc tcgagagcgg cggcaacgcc gacgacccgc
     1321 gcgtgttcaa cggtggctgc aaggaatcca ccgcggtcgc cgcgcagtgc accgacggct
     1381 ggcagttcgt tgacgcgagc gccggctcac cggcggacca cgcctactac ctcgagatgc
     1441 gcgatcgctc gggcttcgac atggacggca agggcgagaa cgatcgcgag ccgatcgcgt
     1501 tcctgcccgg cctcctgctg acctacaccg acgaggcgca cggctacggc aatgcgggca
     1561 cgccggatcc gccggcccag tccccgctcg actcgcagcc gcaggccggg aacaacaccc
     1621 cgaacctgaa tgacgcggcg tggaccgccg cagccggcga caaccacttc tccgactccg
     1681 gcgcgggctg gacggacaac tacaaggacc cctcgagcgc cgacggcttc tggcacttca
     1741 cctgggactg cctgacattc gacgtgctca acatgaccgg caccaacatc ggtccgagcg
     1801 tggtcccgcc gtacaacctg tccggcgaca tccggttcac gatgggcaac ggctgcgccc
     1861 gcttcgacta cggtcacggc ggcccaggcg gacccgtcaa ccagccacca acggccgtcg
     1921 ccgaggcgcg accgaccacg gtcggcaccg gcgagccggt caccttcgac gccagcgggt
     1981 cgttcgacga ccagcaggcg cccaacgagc tcacctacgc ctgggacctc gacgacaacg
     2041 gcacgtacga gacgccgggc cagaccgtga cccgatcgtt tgcggtcgcg ggaacgtacg
     2101 acgtcagcgt gcgcgtcacc gacgctggcg gcctgtccaa cgccgacacc gtcaccgtga
     2161 cggtcgaggg cgtcgaggcc tgcccgccgt tcacgcccac gggcagctcc tcggccgact
     2221 tcgaatccgg cccggcgggc tggacgcccg acaccgcgca ggccctgcct gccagcgtga
     2281 actggcaggt tgcgcccgat cccctcgccc acagccccaa cagctcgttc ttcagcgacg
     2341 ccgtcggcgt ggacctcaag gacgaccgtc tcgtctctcc gccgatgaac ttgtcgagcc
     2401 agtcccagct tcgcttctgg catcgattcg ggttcgaggc cgagttcgac ggcggcgtgc
     2461 tcgaggtgag caccgacgac gggtcgacgt gggtcgacgt gctcgccggc ggcggctcgt
     2521 tcgcggaggg cggctacaac agcgcgatct ccgcggcgta cggcagcccg atcgcgggtc
     2581 ggtccgcctg gaccgggccg caggagggcg ccacgaatcc gatgacccgg gtcgtcgtca
     2641 acctcggcgc gttcgccggc gacgacgtcc gcgtccggtt ccgccttgcg accgatccgt
     2701 tcggcgcggg cgcgttgccc ggcgtcggct ggtggatcga cgacgtgtcg ttcaccaagc
     2761 tcctggagcc ggcaacctgc gacaaccacg ccccggtcgc ggttgatgat gccgcgacca
     2821 ccacgaccgg cacgcccgtg acggtcaacg tgctcggcaa cgacgccgac cccgacgccg
     2881 atgcgctctc ggtcgacgac gtcgggagcc cggcccacgg gaccgtcacc gacaacggcg
     2941 acggcaccgt gacctacacc ccgaacgccg gcttcaccgg ctcggaccgc ttcacctacg
     3001 acatcgtcga cgggcgcggc ggcagcgaca cgggcaccgt gtcgatcacc gtcagctcgg
     3061 caggcaaccg cccaccggtg gccgccgacg actcggccac gacggtcgag gacgactcgg
     3121 tctcgatccc cgtcctcgac aacgacaccg atcccgacca cgacacgctc atggtcagcg
     3181 acttcagcga tcccgcgaat ggctccgtgg ccgtcgacgg cacggggctc cggtacacgc
     3241 cggacgcggg cttcgtcggg acggatacgt tcacctatga cgccgccgac ggacgcggtg
     3301 gcttcgacac ggcgaccgtc acggtcaccg tcaccgaggc gccgaacggg gatccgaacg
     3361 cggtcaacga cgtcgcctcg acgacccgcg acacatcggt ccggatcgtc gtggtggcca
     3421 acgacaccga tcccgacggc gacaacctgt cggtgaccgg tgccacgaat ccgccgcacg
     3481 gcacaaccgt gaaccacggc gacgggaccg tcacgtacac gcccgattcc gggttcaccg
     3541 ggacggacac gttcgaatac accatctccg atgggcacgg cgggagcgac accgcgaccg
     3601 tcaccttgtt cgtcaacgcc tcgagtgaca cggccaaggt caacggcggc ggctggttca
     3661 cgacgggcgg atcgaggtcg agcgtgaacg tcaacgccca ggtcaagaac ggcctggcca
     3721 agggcaaggt cggctacgac ctcgacggtg ggatctcgat caagggcaac gtcgatgcgc
     3781 tgcgcatcac gggctcggcg gcggacgtca ccggctcgtg cacccgcgca aacggggctg
     3841 cctgcacctt cgaggtccac gtcgtcgaca acgccgagcc cggcgccggc tgggacacct
     3901 tcagcatccg tgtctacgac gcggcggggg ccctcatcca cgaggcctcc gggatcctgg
     3961 aaggcggcaa cctccaggtc cactgaccgg ggcaatcacc gcagtcaccg cagtcaccgc
     4021 gcggcgggga gcagagcgac acctgctccc cgccgcggca tgtcacgccc ggagacgctt
     4081 gccggctgct tgcaggccgg gtctagactc ggcctctcga ctcgtcccgt ggggagggac
     4141 ccggtgcgtc gtcgcggccg tctggcgcgg ttcctggcca gccttgccct cttcgggctc
     4201 ggcttctcac tcggcgcgca aacggcgatt gccgtcaatc ccgagctgac cttgcggccc
     4261 gccaagggcg cggccggctc cacgttcgcc gcgacctacg aggacatggt tcccgcgggc
     4321 gcgctgtgca ccgtgggcac ggtcgacttc tggtgggatg ctgccgggcc tggttcgggc
     4381 aaggccggcg cgaagaagct cggctccgcg acgatcgcgg tacaggattg cgtcgcggtc
     4441 gctcctgcgc tgaaggtccc caaagcgtcc tgtggccggc acgtcgtgta cgccttccgg
     4501 tcgcgcaatg gcgaccccgt gcccacctcg gtgacgagcg cggcattcac ggtcaacggt
     4561 tgtgcgacag cgtcgccggc gccgaccgct cggccgacgc ctgccccgac acccagcgct
     4621 ccgccgttgc cgacggccac gcctggtgcc gagccgtcaa ccgcgccctc ggcgctccca
     4681 tccggatcgc cggcagcgag caacccggca gcgcccacgc ccgcgtcggt cggcccgctg
     4741 gtgacaccgc cgccggtccc gcccccaccg ccgcccccgt cgagcgtgga tctggccggg
     4801 atcctcgtcc tcgtcatcct cggattgctg ccgatcggcg tctgggcagc agcggtccac
     4861 gtcaagctcg gaatcgtctc gggcggcgca tcaaagtggg ccgggctcgt cctcgccgtc
     4921 atcctcggcg gcgggctcac gtgggcgggc tcgctcaagt tcgggctgaa gcccgtcccg
     4981 gtgtcggccg aattcgagta cacgggcgga tcgcagttct gggtcgtccc cgaaggggtg
     5041 gaggtcgcgc acgtctgggt gatgggtgcg gcaggaggcg cgggatgtcc gctggagccg
     5101 ctcgacttcc agggccatcc gcacaacgcc ggcggctccg gtcacgagat cacatcgagc
     5161 atgcctgtca cgccaggcca ggtgatccag gtcaacgtcg gcggcaaggg ccacgacggc
     5221 gagtgcgacg gatcgttcgg cgaagcgtcc aacgccctcg gtggattcaa cggcgggggc
     5281 gccgggggtg ccggaggcaa gggcatccct tctctcgttt cgtacaacgg cggcggaggc
     5341 ggtggcgcct ctgatgtgcg tcatgcgccg tacggcctgg cggcccgtga cgtcgtggcc
     5401 ggcggtggtg gcggtggcgg aggcgccgcc agctccgacg actccatgaa gggcggacca
     5461 ggaggttgcc cgaccggcga agcgggtccc ggagccaacg gacagggcgg tggtggaggg
     5521 atcctgacga tgggtggcat cggtggcgcc aacggcggca atggcggcct gagcggcgaa
     5581 tggggcacgt tcggtctggg cggcgccggg gccgaccgcg gcagcggcca gacggccgga
     5641 ggcggcggtg gcggtggttg gaatggtggc ggtggcggcg gatcaggcgc gggcagccag
     5701 tggggcggtg gcggcggtgg tggcggctgt cggtggccgg aagggggcga ctacgatgac
     5761 accaacgccg tgtccttcgg gaatggccgc gtcgtgatcc agtacacgca gtacgtgtcg
     5821 atcttctggg gcctttgatt cagtcgcggg cgagcatccg caacgcggcc ttgacgcgct
     5881 cctccagcga agcgtcgacg cctgccatcc cgatggcgac gcgcgacgcc tcccgcgctt
     5941 ccgggagcga atagccgagg gcctggagcg cgccgacgac atcgccttcg ccgcccgccg
     6001 gagatccggc aaccgcgatg gccgccacgc cagctgcggc gaccttctcc ttgagctcga
     6061 agatcacgcg ctcggcgagc ttcttgccga tgcccgggat cgagacgagc acggcctggt
     6121 cctgctgcat gatcgcgagc tggagctccg cggtcgggcg cgagccgacg atggccatcg
     6181 cgaccttcgg gccgacgccg gtcaccgtga gcagcagccc gaagaacccg agctcctcga
     6241 ggccgcggaa gccgtacagc gcctgcaggt cctcgcggac gacgtggtgg gtgaacagct
     6301 tcagccggcc gccaggcggc gccgacgcga gcaccgatgg cgagacgtac acgcgatagc
     6361 cgaccccgcc gacctcgacg atgacgctat cgagcgcaac cgccgcgacg atgccgtcga
     6421 gcgaggcgat caccgcttcg gacgcgcggg ggtcttctcg agcgcgaggg cctcgcgcac
     6481 cgcgcgttcg tacgaggtgc cgcccgcccc cgtggcaacc atcgggttga ccgaggcgcg
     6541 atccaagacc gccgagcggc gctccacgct tcccggtcgc tcggcgttgg ccgcccagat
     6601 cgcgatggcg agcgcgtcgg aggtgtcgtc ctgcttcggg gcagcgggca ggctcaggca
     6661 caccgcgacc atccgcccga cctgctcctt gtccgcgcgc ccggaaccgg tcacggcgag
     6721 cttcacctcc gccggcgtgg cctcgcgcac tggcacctcc gcctgggcgg ctgcaagcag
     6781 caccacgccc cgcgcctgcc cgacggcgaa cgccgtctgc acgttgcgcg agaagaacag
     6841 ccgctcgacg gccatgaggt ccggctt
//
LOCUS       DATBSW010000244         4902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_587454, whole genome shotgun sequence.
ACCESSION   DATBSW010000244 DATBSW010000000
VERSION     DATBSW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587454"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VIF63_06760"
     CDS             <1..999
                     /locus_tag="VIF63_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8239118.1"
                     /translation="VLYQNVFWFYSHPAVYVMVLPAMGVISEVLPVFSRKPLFGYKAF
                     IFATAGIGALGFSVWAHHMFTTGAVFLPFFALMTFLIAVPTGVKMFNWIFTMFRGQLM
                     FSTPLIFAVGFLTMFLIGGINGAFSAAVPVDFALHDTYWVVAHLHYVLFGGSVFGVFA
                     GLYFWFPKMTGRMLDETLGKVHFVLMFVGFNLTFFPMHMLGIKGMPRRIADYDAFAGW
                     NDWNLLATIGGFMIAASMLPFLWNVFVSLRNGKIAGDDPWEANTLEWATSSPPPPYNF
                     DRLPEIRSERPVFDARHGAHAGHGGHGRGEEEEAPKALEEGGHDDMGPTRHMGTTT"
     gene            996..1622
                     /locus_tag="VIF63_06765"
     CDS             996..1622
                     /locus_tag="VIF63_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8239119.1"
                     /translation="MTADAPAITAGHGAPHAPSGHAQRGISNPILGMILFITSEVMFF
                     SGLFAAYFATRAAHTEWPPAAFADILDPLGPIIIATILLIASSFTCQFAVWSIRRGDR
                     TGFIRNIAITFVLGIIFLGMQVYDYGLLFGEGMTLASGPFGTTYFTLTGFHGAHVFGG
                     VLMLGVLLYRGMSGQFSAKHHDAVEAVSLYWHFVDVVWILLFSILYLI"
     gene            1637..1822
                     /locus_tag="VIF63_06770"
     CDS             1637..1822
                     /locus_tag="VIF63_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239120.1"
                     /translation="MLRRFRNSIAIGIACVVTAGAYASLAKPFGYHIEWAGVTMLIAL
                     GIAMGLMVAVLDTGSGE"
     gene            1836..2942
                     /locus_tag="VIF63_06775"
     CDS             1836..2942
                     /locus_tag="VIF63_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8239121.1"
                     /translation="MEPTAAPAKDIIQTLLDVAAAFIIPDWRALILLLPIALALLFAV
                     WFLFTVRKFATLGPRRRAPARVQPVTPASIHMPGGSSAPILVAFGAAFLFVGLVVGGI
                     ALWIGVVAIVVTLVAWFREAMRDYDQIESTQLVPVVVHEGPPPGVHMPGPSIRPLLGA
                     LGSAALLGGLVVGGWVLILAVVFLVYTLIGWLIDFTAEYRKVEEADKTGHLENIPDRK
                     LPLRTLQVFAVLFALVGLYQLGIFPPPAPAAGGVPGASGAPAASGGATTGCGPAAPGA
                     LALVAKDAKFNTKALDAPAGKPFAIFLCNEDGAVVPHDVEIRSSDGATLQKQPPTPGG
                     TSSTYQYTPLETGAYVFICSIHPIAQMTGTLTVN"
     gene            3045..3557
                     /locus_tag="VIF63_06780"
     CDS             3045..3557
                     /locus_tag="VIF63_06780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8239122.1"
                     /translation="MIDGPGASSSPSTSTIARGQPAPKIIGTTLDGAAFDLATLRGRP
                     VIVNFWGPSCIPCRDEFPLFAAKLAQHVGDGLAIVGVLMDDPAPPARDFMVEYGATWP
                     TVDDAEGAIRGTYRVAARPQSYFIDRDGVLRSIQVGQLTDADFERQLAMISGPAAGAS
                     SGPAGATITP"
     gene            3554..4495
                     /locus_tag="VIF63_06785"
     CDS             3554..4495
                     /locus_tag="VIF63_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239123.1"
                     /translation="MIEVSGLRKAYGGRIVLDGVDLVVRPGEIVALLGPNGAGKTTTV
                     EIVEGYRAGDAGTVRVLGEDPRRGGPALKARVGLMLQTGAGLEPRATPRDLVRLYAAF
                     HAGGRAPDELIETVGLAAVANTRVRRLSGGERQRLALAIALVGDPEVLILDEPTAGMD
                     PEARRATRALVAGLRGEGRTILLTTHDLGDVEHLADRVAILAAGRIVADDAPEVLLRG
                     GTVAVQFRLHAPPRPAAVEALGRRIVELSPESRLRAVTDTAFEIAPGAASADVVAEIA
                     GWALREGVLIVELRAGAGSLEERYLELTGDRAVEDVE"
     gene            4492..>4902
                     /locus_tag="VIF63_06790"
     CDS             4492..>4902
                     /locus_tag="VIF63_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8239124.1"
                     /translation="MSRPAPARRAVAALALQELRLTARRGENVLVTLIIPVAVLLFFA
                     GTAVVPGVAGRPIDSVLPGAIALAIIATGLVNLGIATAYERSYGVLKRLGGAPLPRLG
                     LIAAKVTAVLALELVQVVLLVAVAYAVFGWSPGPG"
BASE COUNT          633 a         1773 c         1651 g          845 t
ORIGIN      
        1 gtgctctacc agaacgtgtt ctggttctac tcgcaccccg ccgtgtacgt gatggtcctg
       61 ccggcgatgg gcgtgatcag cgaggtcctg ccggtcttca gccgcaagcc gctgttcggc
      121 tacaaggcgt tcatcttcgc gactgcgggc atcggcgcgc tggggttctc ggtttgggcc
      181 caccacatgt tcacgaccgg cgccgtgttc ctgccgttct tcgccctgat gaccttcctc
      241 atcgcggtcc cgaccggcgt gaagatgttc aactggatct tcacgatgtt ccgcgggcag
      301 ctcatgttct cgaccccgct gatcttcgcg gtgggcttcc tgacgatgtt cctgatcggc
      361 gggatcaacg gcgccttcag cgccgctgtc ccggtcgact ttgccctgca tgacacgtac
      421 tgggtggtcg ctcacctcca ctacgtgctg ttcggcggct cggtgttcgg cgtcttcgcc
      481 ggcctgtact tctggttccc gaagatgacg ggccggatgc tcgacgagac cctgggcaag
      541 gtccacttcg tcctgatgtt cgtcggcttc aacctgacgt tcttcccgat gcacatgctc
      601 gggatcaagg gcatgccccg gcgcatcgcc gactacgacg cgttcgccgg ctggaacgac
      661 tggaacctgc tcgccacgat cggcggcttc atgatcgcgg cgtcgatgct gccgttcctc
      721 tggaacgtgt tcgtctcgct gcgcaacggc aagatcgccg gcgacgaccc gtgggaggcc
      781 aacaccctcg agtgggcgac ctcgtcgccg ccgccgccgt acaacttcga tcgcctgccc
      841 gagatccgct cggagcgccc cgtcttcgac gctcgccatg gcgcccatgc gggccacggc
      901 ggccatgggc gcggtgagga agaggaagcg ccgaaggccc tcgaggaagg tggccacgac
      961 gacatggggc cgaccaggca catggggacg accacgtgac ggccgacgca ccggccatca
     1021 ccgccggcca cggggcgccg cacgcgccgt cgggtcacgc ccagcgcggg atcagcaacc
     1081 cgatcctcgg gatgatcctg ttcatcacct ccgaggtgat gttcttctcg ggcctgttcg
     1141 ccgcctactt cgcgacgcgg gcggcgcaca ccgagtggcc accggcggcg ttcgcggaca
     1201 tcctcgaccc gctcgggccg atcatcatcg ccacgatcct gctcatcgcg agctcgttca
     1261 cctgccagtt cgccgtttgg tcgatccggc ggggcgaccg gaccgggttc atccggaaca
     1321 tcgcgatcac cttcgtcctc gggatcatct tcctgggcat gcaggtctac gactacggcc
     1381 tgctgttcgg ggagggcatg accctcgcct cgggcccgtt cgggacgacg tacttcacgc
     1441 tcaccggctt ccacggcgcc cacgtcttcg gcggcgtgct gatgctcggc gtgctcctct
     1501 accgcggcat gtccggccag ttctcggcga agcaccacga cgccgtcgag gcggtgtcgc
     1561 tgtactggca cttcgtcgac gtggtctgga tcctgctctt ctcgatcctc tacctgatct
     1621 agcgagggat cgaccgatgc tccgccgatt tcggaactcg atcgccatcg ggatcgcctg
     1681 cgtcgtgacg gcgggcgcct acgccagcct ggcgaagccg ttcggctacc acatcgagtg
     1741 ggccggggtg acgatgctca tcgccctcgg catcgcgatg gggctcatgg tcgccgtcct
     1801 cgacaccggc agcggcgagt agcctcccgc cctcgatgga gccaaccgca gctcccgcca
     1861 aggacatcat ccagacgctc ctcgacgtcg cggcggcgtt catcatcccc gactggcgcg
     1921 ccctgatcct gttgctgccg atcgcgctcg cgctcctgtt cgcggtctgg ttcctcttca
     1981 ccgttcgcaa gttcgcgaca ctcggcccgc gccgccgcgc cccggcccgc gtgcagccgg
     2041 tgacgcccgc gagcatccac atgccagggg gctcgtcagc cccgatcctg gtcgcgttcg
     2101 gcgcggcgtt cctgttcgtc gggctggtcg tgggcgggat cgcgctgtgg atcggggtgg
     2161 tcgcgatcgt cgtcaccctc gttgcctggt tccgcgaggc gatgcgcgac tacgaccaga
     2221 tcgagtcgac gcagctcgtg cccgtggtcg tccacgaagg tccgccgccc ggcgtgcaca
     2281 tgcccgggcc atcgatccgt ccgttgttgg gcgcgctcgg ctctgcggcg ctgctcggtg
     2341 gcctcgtggt cggcggctgg gtgctcatcc tcgccgtcgt gttcctcgtc tacacgctca
     2401 tcggctggct catcgacttc acggcggagt accgcaaggt cgaggaggcc gacaagaccg
     2461 gccatctcga gaacatcccc gatcgaaagc tgccgctgcg cacgctccag gtcttcgcgg
     2521 tcctcttcgc gctcgtcggg ctgtaccagc tgggcatctt cccgccgccg gcgccggccg
     2581 cgggcggcgt tcccggcgca tccggcgcgc ccgcggccag cggcggcgcc acgaccggtt
     2641 gcggccctgc ggcacctggt gcgctggcgc tcgtcgccaa ggacgccaag ttcaacacga
     2701 aggcgctcga tgccccggcg ggcaagccgt tcgccatctt cctgtgcaac gaggacgggg
     2761 cggtcgtgcc ccacgacgtc gagatccgtt ccagcgacgg cgcgacgctc cagaagcagc
     2821 cgccgacgcc gggcggcacc tcgagcacgt accagtacac gccgctggag accggcgcct
     2881 acgtcttcat ctgctcgatc cacccgatcg cacagatgac cggaacgctg acggtcaact
     2941 aaggcaggcc ggccacgctg cagctggacc ggcagcgccg gctcatcctc ctcggactcg
     3001 tagcggccgc cctcgcgaca ctcgcgctgg tccagctccg acagatgatc gatggtcccg
     3061 gcgcctcctc gtcgccttcg acgagcacga tcgcgcgcgg ccagccggcg ccgaagatca
     3121 tcgggacgac gctcgacggc gcggccttcg acctcgcgac cctgcgcggc cggcccgtga
     3181 tcgtcaactt ctggggcccg agctgcattc cctgccgcga cgagttcccg ctgttcgcgg
     3241 cgaagctcgc ccagcacgtc ggcgacggcc ttgcgatcgt gggcgtgctg atggacgacc
     3301 cagctccgcc ggctcgggac ttcatggtcg aatacggcgc cacgtggccg acggtcgatg
     3361 acgcggaggg tgcgattcgc gggacgtatc gcgtcgcggc gcggccgcag agctatttca
     3421 tcgatcgcga tggcgtcctg cgctcgatcc aggtcgggca gctgaccgac gccgacttcg
     3481 agcggcagct ggccatgatc agcggcccgg cggcaggagc atccagcggc cctgccggag
     3541 cgacgatcac gccgtgatcg aggtctccgg cctgcgcaag gcctacggcg gccggatcgt
     3601 gctcgacggc gtcgacctgg tcgtccggcc gggcgagatc gtggccctgc tcggaccgaa
     3661 cggcgccggc aagaccacga cggtcgagat cgtcgagggc tatcgcgccg gcgacgcggg
     3721 cacggtgcgc gtgcttggcg aggacccgcg acgcggcggt cccgcgctca aggcccgggt
     3781 cgggctgatg ctccagacag gcgccgggct cgagccgcgg gcaacgccgc gcgacctcgt
     3841 gcgcctctat gccgcgttcc atgccggcgg ccgcgcgccc gacgagctca tcgagacagt
     3901 cggcctcgca gcggtcgcga acacgcgggt ccggcgcctc tccggtggcg agcgccagcg
     3961 gctcgcgctg gcgatcgcgc tcgtgggtga tcccgaggtg ctgatcctcg atgagccgac
     4021 tgcgggcatg gatcccgagg ctcgacgggc gacgcgggcg ctcgtggccg ggctccgcgg
     4081 tgagggccgc acgatcctgc tgaccaccca cgacctgggc gacgtcgagc atctcgccga
     4141 ccgggtggcc atcctcgccg ccggtcggat cgtcgccgac gacgcgcccg aggtgctcct
     4201 tcgcggcggc accgtggcag tccagttccg gctccacgcg cctcctcgcc ccgcggccgt
     4261 cgaagcgctc ggtcggcgga tcgtcgagct cagccctgaa tctcgccttc gtgccgtgac
     4321 cgacaccgcg ttcgagatcg caccgggcgc ggcatccgcc gatgtcgtcg ccgagatcgc
     4381 gggctgggcg ctgcgcgaag gggtgctcat cgtcgagctg cgggccggcg ccggctctct
     4441 cgaggagcgc tacctcgagc tgaccggcga tcgcgccgtc gaggacgtcg agtgagcagg
     4501 ccggcaccgg ctcggcgggc ggttgccgcg ctcgcgctgc aggagctgcg cctgaccgcg
     4561 cggcgcggcg agaacgtgct cgtgaccctg atcatcccgg tcgcggtcct gctgttcttc
     4621 gccgggaccg ccgtcgtgcc gggcgttgcc ggccgcccga tcgattcggt cctgccgggg
     4681 gccatcgcgc tcgcgatcat cgccaccggg ctcgtcaatc tgggtatcgc gacggcctat
     4741 gagcgctcgt acggcgtgct gaagcgcctc gggggcgcgc cgctgccgcg cttgggactc
     4801 atcgccgcga aggtgaccgc ggtccttgcg ctcgagctcg tccaggtcgt cctgcttgtc
     4861 gccgtcgcgt acgccgtctt cggttggtcg ccggggccgg gc
//
LOCUS       DATBSW010000245         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1187392, whole genome shotgun sequence.
ACCESSION   DATBSW010000245 DATBSW010000000
VERSION     DATBSW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187392"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..577)
                     /locus_tag="VIF63_06795"
     CDS             complement(56..577)
                     /locus_tag="VIF63_06795"
                     /inference="COORDINATES: protein motif:HMM:NF022167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239125.1"
                     /translation="MLTAAQAVDLCEALEGAGVRYWVIGGWGVDALLGRETRPHKDLD
                     ILAVRDDLAQLRGMFEQERLAVSHIWEESRWIDDIGEPWPTAFVAEDPLRRAIDVHLI
                     GAPSGGTIVQHYDNPWSFPESFEATGTIAGRAIPCVSAATQISMHAGYTLPDEQQLDL
                     DLLRSQVAFTGTD"
     gene            complement(582..1157)
                     /locus_tag="VIF63_06800"
     CDS             complement(582..1157)
                     /locus_tag="VIF63_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8239126.1"
                     /translation="MLIVISGPIASGKSSVASGVAQALRRHDVATAVVDLDEVHAGQT
                     QGSGEPEGDAARWEAARRAAAALADSFIAEGIVAVIVEGSFQTQRDRRVFTETLGSRP
                     DALYVSLRVSYEEALRRAQSDPTRGISRDPRFLRRYYADRAPSSVPKTDLVIDTESMA
                     EKQVVALVARAVLARLRETTPHVTLGALTES"
     gene            1353..2009
                     /locus_tag="VIF63_06805"
     CDS             1353..2009
                     /locus_tag="VIF63_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8239127.1"
                     /translation="MIFDLDGTLVDTVNARIEGWLEALEAAEIRAARVDIAPMIGMDG
                     RRLAREVAFAVGRDLTPDEVDATDRAAGEAFDRLNRRPRTLPGVHEAIAALDQAGIPW
                     VVATSSRREQVAVSIKALGLAREPTIVDGSHVEHAKPAPDLLLLAASRMGVDPVGCWA
                     VGDSTWDIRAAVAGGMTAVGVIEGSAVPGAELKEAGATMVIGSLVDLAELIILVGKPG
                     "
     gene            complement(2307..3074)
                     /locus_tag="VIF63_06810"
     CDS             complement(2307..3074)
                     /locus_tag="VIF63_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEY8239128.1"
                     /translation="MSAAMLVPEVGPAQRASLLGRTNPLVKLGIALAWLLGMGLTLDV
                     RPPLILVLVALLAGLLAGSIELPVLARRVAPFGLAALSIALANLLFSTHNPDPLATEL
                     LRIGPLRITQEAVDAAAGLGARVASIVSVGAVFALTTDATRLVDSLVQLGHVSPRFAY
                     GALAAYQAVPRLADDLGTIRAARRLRGMREWHPRIILGLLVRAIRHADQLAIAMDARG
                     FGSGPRTTYRPIAWTTLDAVVGVAALAILVFALLASA"
     gene            complement(3071..>4156)
                     /locus_tag="VIF63_06815"
     CDS             complement(3071..>4156)
                     /locus_tag="VIF63_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8239129.1"
                     /translation="PGVLVLDEPTACLDPDGAARVMAIVEEIRSRRAATVVLVEHRAA
                     LAWPVADLVLALDDKGRLIDFGPPADVLKRSGAKLERAGIWLPPSAGARTAAAKPAAT
                     ARPAGAVLGSIPILEMAGVRFGFERDHPVLRSVDLMVSPGERIALVGANGSGKTTLLR
                     LALGLLRPVAGDVRLGGREPSRIPPVAVSRLAGYVVQDPELGFLGETVRNEVELGLEP
                     EQLPYAHDLCDRLRLPLAEFGDRSPYQLSGGEQRRLSLVTGLSRRPLLLALDEPTYGQ
                     DRRGHEALVAAIDELVGQGSSMLAATHDEQFVRDAANRRIELVEGWIVADDLVRRGDP
                     VLPGDPMPPGDPMPGDPMPPADAVLPA"
BASE COUNT          673 a         1374 c         1507 g          602 t
ORIGIN      
        1 caccaggatc ctcgacatcg ggacaaccct atcgagtgtc gcgcgcggcc gcggctcagt
       61 cggttccggt gaatgccacc tgacttcgaa ggagatcgag gtcgagctgt tgctcgtcgg
      121 gcagcgtgta cccggcgtgc atgctgatct gggtcgctgc cgacacgcat gggatcgccc
      181 tcccggcgat cgttccagtc gcctcgaagg attcggggaa tgaccacggg ttgtcgtagt
      241 gctggacgat cgttccgccg gatggggcac cgatcagatg gacgtcgatg gcccgacgga
      301 gcgggtcttc ggcaacgaat gcggtgggcc agggttcacc aatgtcatcg atccagcggc
      361 tctcctccca gatatgactc accgcgagac gctcttgctc gaacatgcca cgaagctgcg
      421 cgaggtcgtc acggacggcg aggatgtcca ggtccttgtg cggccgcgtc tcgcggccga
      481 ggagggcgtc cacgccccag ccaccaatca cccagtagcg cacgcctgcg ccttcgagcg
      541 cctcgcagag gtcgaccgcc tgagccgcgg tcagcatgtc ttcacgactc agtcagcgcg
      601 cccagggtca cgtgaggggt cgtctcccgg agacgggcga gaacggctcg ggcgacgaga
      661 gcgacgactt gtttctcagc catcgactca gtgtcgatga cgaggtcggt cttgggcacg
      721 cttgaagggg cccggtccgc gtagtagcga cggagaaacc tgggatcacg cgagatgcct
      781 ctggtcggat cggactgggc gcgtcgcaga gcctcctcat acgagacgcg aagggacacg
      841 tagagcgcat cggggcgaga gcccagggtc tcggtgaaca cccgtcggtc gcgctgcgtc
      901 tgaaaggatc cctcgacgat gactgcgacg atgccttcgg cgatgaagga gtctgcgaga
      961 gcagcggcgg cgcgacgagc cgcttcccac cttgccgcgt ctccctcggg ctcgccggaa
     1021 ccttgcgtct ggcccgcatg cacctcgtcg agatcaacca cagccgtcgc tacgtcgtgt
     1081 cgtcgaagcg cctgtgcaac accggaggca accgaactct tgccgcttgc gatcggaccg
     1141 ctgatgacaa tgagcacgca tcgattctgc gcgccccgct cggcctcggt cgccgtgatg
     1201 acacgagccg gtcttcgcct ggacgtcgtc cagataggct tggaatgaca tcgtcgacag
     1261 gatgaactgg cccggcacgt gataccagcg gatgcgtcga tcgaagctcg tggagattgg
     1321 ccttgagaaa cgcgctatct ggcccagaag gcgtgatctt cgacctcgac ggcacgctcg
     1381 tcgacaccgt gaacgcgcgc atcgaaggct ggctggaggc gctcgaggcg gccgagatcc
     1441 gcgccgcgcg cgtcgacatt gcgccgatga tcggcatgga tggccggcgg ctcgcacgcg
     1501 aagtcgcgtt cgctgttggc cgggacttga cgcccgacga ggtcgatgcg accgaccgag
     1561 cggcgggcga ggcgttcgat cgactgaatc gccgaccacg gacgctgccg ggggtccacg
     1621 aggcgatcgc ggcgctcgat caggcgggga tcccgtgggt cgtcgcgacg tcgagccgcc
     1681 gggagcaggt ggccgtctcg atcaaggcgc ttggcctcgc ccgcgagccg acgatcgtcg
     1741 acggcagcca tgtcgagcac gccaagccgg cgccggacct gttgctgctc gccgcttcgc
     1801 ggatgggcgt cgatcctgtc ggctgttggg cggtcggcga ttcgacgtgg gacattcggg
     1861 ccgccgtcgc gggcggcatg accgcggtcg gcgtgatcga aggatcggcc gtgcccggcg
     1921 cggagctcaa agaggccggc gcaacgatgg tcattgggtc gctcgtcgat ctcgccgagc
     1981 tgatcatcct ggtaggtaag cccggctaga tcctctgcct cgccggcggc ggcgtccgat
     2041 cgacgaagtg cgcctgcatg atcgcgtggc gatcatttct gggggttcgc agcggatttc
     2101 atgtctgccg atccggatga cgccgcgttc cagctggcaa tcagcgtcaa ctgaacgtgg
     2161 cctgatcgtg tcggcagtcg gtaacgtcca agcgtgggac tcgatttggg aacctgcagc
     2221 gccaaatcgc ctcccaaccg ccagaaaaga tcgcccggcg atcatctggt cgcggatcgc
     2281 ggaacgcgcc gggccgtccg cccgatctag gcggaggcga ggagcgcgaa gaccaggatc
     2341 gcgagggcgg ccacgccaac cacggcgtcg agcgtggtcc aggcgatcgg gcggtaggtc
     2401 gttcgagggc cgctgccgaa gccacgggcg tccatcgcga tcgcgagctg gtcggcgtgg
     2461 cggatcgcgc gcacgagcag cccgaggatg atgcgcggat gccactcacg catgccgcgc
     2521 aggcggcgag cggcgcggat cgtgccgagg tcgtcggcga gccgtggcac ggcctggtac
     2581 gccgcgaggg cgccgtacgc gaaccgcggc gacacatgcc ccagctggac cagcgaatcg
     2641 acgaggcgcg tcgcgtccgt cgtgagcgcg aacaccgcac cgacgctcac gatcgaggcg
     2701 acccgcgcgc cgaggcccgc cgcggcgtcg accgcctcct gggtgattcg cagcgggccg
     2761 atgcgcagga gctcggtcgc gagcgggtcg gggttgtgcg tcgagaagag caggttcgca
     2821 agcgcgatgc tgagcgcggc gaggccgaac ggcgcgacgc gcctggccag gacgggaagc
     2881 tcgatcgagc cggcgaggag accggcgagc agcgccacca gtaccaggat cagcggtggc
     2941 cgaacatcga gcgtcaggcc catgccgagc agccacgcga gcgcgatgcc gagcttgacc
     3001 agcgggttgg tgcgcccgag caggctcgcg cgctgggcgg gtccgacctc ggggacgagc
     3061 atcgcggcgc tcatgcgggc agcaccgcgt cggcgggcgg catcggatcg ccgggcatcg
     3121 gatcgccggg cggcatcgga tcgccaggca ggactggatc gccgcgccgc accaggtcgt
     3181 cggcgacgat ccacccctcg accagctcga tccgtcgatt cgcggcgtcg cggacgaact
     3241 gctcgtcgtg cgtggccgca agcatcgagc tcccctgccc caccagctcg tcgatggcgg
     3301 caacgagcgc ctcgtggccg cggcggtcct ggccgtatgt cggctcgtcg agcgcgagca
     3361 ggagcgggcg gcgggagagc ccggtcacga gcgagagccg gcgctgttcg ccgccggaga
     3421 gctggtatgg acttcggtcg ccgaactcgg cgagcggcaa ccgaagccga tcgcacaggt
     3481 cgtgggcata cgggagctgc tcgggctcga ggccgagctc gacctcgttg cgcaccgtct
     3541 caccgaggaa gcccagctcg ggatcctgga cgacgtagcc cgcgagccgc gacacggcga
     3601 cgggcgggat tcgggacggc tcgcggccgc cgagccgtac gtctccggca accgggcgaa
     3661 ggaggccgag cgccagccgg agcaaggtcg tcttcccgct gccgttcgcc ccgaccagcg
     3721 cgatgcgctc gccgggcgag accatgaggt cgaccgagcg aaggacggga tgatcgcgct
     3781 cgaacccgaa ccgcacgcca gccatctcga ggatcgggat cgatccgagg accgcgccgg
     3841 ccgggcgagc tgtcgccgcc ggcttggcgg cggcggtgcg cgccccggcg ctgggaggga
     3901 gccagatccc cgctcgttcg agcttcgccc cggagcgctt gagcacgtcc gccggcggcc
     3961 cgaaatcgat cagacggccc ttgtcgtcga gggccagcac gaggtcggcg accggccacg
     4021 cgagcgcagc tcgatgctcc acgagcacga cggtggccgc ccgccgcgag cggatctcct
     4081 cgacgatcgc catgacgcgg gcggcgccgt cgggatcgag gcaggccgtt ggttcgtcga
     4141 ggaccagcac gcccgg
//
LOCUS       DATBSW010000246         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1151510, whole genome shotgun sequence.
ACCESSION   DATBSW010000246 DATBSW010000000
VERSION     DATBSW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151510"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VIF63_06820"
     CDS             <1..761
                     /locus_tag="VIF63_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239130.1"
                     /translation="AHAKRAPGDASATPAGTPRASRAGNDTPDRLMTERNPRTAAEVA
                     TRMAEDGASDPAPRRGDPWGWARVLAKIALAIVILGILLLAALYLMLMSSMPKAPTST
                     VAPVAWASEVIELSPDAPVVHGRITLTMVSIPSTGFRVGVNAGVPSVTDQSPAAALSG
                     PAVRVSAEPAPGTNSSCFAPCELQVAEAFDCGSDGCRMVMDVTIELVPTATGSAKPVT
                     LTVSSGLTGTSAKPLASPFDIDLAFEPAAAPRGT"
     gene            763..2316
                     /locus_tag="VIF63_06825"
     CDS             763..2316
                     /locus_tag="VIF63_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239131.1"
                     /translation="MSAPKRRLRQSAVLVVFALSVVATAATSPSYTTIQFAPASDTLH
                     LDAEHPAALARIVVKVNAEGTSDSATYGVRLRIAGITPEAGGTMLPFDVAAARFIVMS
                     AVPGGPMPGGLVPGLPTDEPNLGPVPSAWQAEAQVPGEVQLPMQCGAGPCERAFWLVA
                     QLVDGHVGAVSVDWTVSGSLGFSAQTWPSGAGGSIEIDPPIFVAGPIPQLVTTTEPEE
                     LRLGPTSPAAARVVEVRIGADAITGDGSAVGTMSVDLSSRPGASYDSEHRPIVHVYPL
                     LGFDEPPGTGFLPDPADDGVDPFADCEPGVDCVRRFLVTFAWNGVDGQDALYDWNLRV
                     RRLDLIRAWSTPVELSAAIVRRIDVAPGSASKVVHLGGDEQTTPGGSEPEPQIRVGLE
                     GSTPAEDRLARLLPVPAVMTYRVHVLRNDPAATQDPREFSNVQLPGERIGAHVHLDFF
                     RGEDSVVTNPLAACRIGDVCDDMTISIIRVGPLDKDPLPPVTAHWSLDILTYSYVDIP
                     VELSVDRRP"
     gene            complement(2294..>2686)
                     /locus_tag="VIF63_06830"
     CDS             complement(2294..>2686)
                     /locus_tag="VIF63_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239132.1"
                     /translation="PRYMGLLIPHHYEHHVLRKPAAEWPDAVNRAFAAINPSIYVPIQ
                     GPSELGAGGVLLTWDRTDDLERIMVPTLVIAAQHDTMDPEFMRMMSGRLPRGSFLLCE
                     NGSHMTMYDDPEPYFAGLLGFLKDVDRR"
BASE COUNT          380 a          969 c          917 g          420 t
ORIGIN      
        1 tcgcgcatgc caagcgtgcg ccgggagacg cctcggcgac accggccggc acgccacgag
       61 cctcccgcgc tggcaacgac acccccgacc gcctgatgac cgagcgcaac cctcgcacgg
      121 ccgcggaggt cgcgacgcgc atggcggagg acggagcgag cgacccggca ccgaggcggg
      181 gcgacccgtg gggttgggcc cgggtgctcg cgaagatcgc cctcgccatc gtcatcctgg
      241 gcatcctcct cctcgcggcg ctctacctga tgctcatgtc gagcatgccc aaggccccga
      301 ccagcaccgt cgcacctgtc gcctgggcca gcgaggtgat cgagctgtcg ccggatgcgc
      361 ccgtcgttca cgggcgaatc acgctcacga tggtctcgat cccatcgacg ggattccggg
      421 tgggggtgaa cgcgggcgtg ccgtcggtga cggaccagtc gccggccgcc gccctctcgg
      481 ggcctgccgt gcgcgtcagc gccgagcccg cacccggcac caactcgagc tgtttcgcgc
      541 cctgcgagct gcaggtggcc gaggctttcg actgcggcag cgatggctgc cggatggtca
      601 tggacgtgac gatcgagctc gttccgaccg cgaccggctc ggccaaaccg gtcaccctca
      661 ccgtgtcgag cgggttgacg ggcacgtcgg cgaagcccct cgcctcgccg ttcgacatcg
      721 acctcgcctt cgagccggcc gctgcgccgc gcgggacctg acgtgtcagc cccgaaacga
      781 cgactccgcc agtccgcggt cctggtcgtc ttcgccctga gcgtcgtcgc gacggccgcg
      841 acgagtccga gctacacgac catccagttt gctccggcat cggacacgct ccacctcgat
      901 gccgagcacc ctgcggccct cgcgcgcatc gtcgtcaagg tcaacgccga gggcacctcg
      961 gacagcgcga catacggcgt gcgcctccgg atcgccggca tcacgcccga ggccggcggg
     1021 accatgctcc cgttcgacgt cgcggccgcg cgcttcatcg tgatgtcggc ggttcccggc
     1081 ggcccgatgc cgggcggcct ggtgccgggc ctcccgaccg acgagcccaa cctcgggcca
     1141 gtcccgtcgg cgtggcaggc ggaggcccag gttcccggcg aggtccagct tcccatgcag
     1201 tgcggcgcgg gaccctgcga gcgagcgttc tggctcgtcg cccagctcgt cgacgggcac
     1261 gtcggcgccg tctcggttga ttggaccgtc tccggctcac tcggcttttc ggcccagacg
     1321 tggccgtccg gcgccggcgg gagcatcgag atcgacccgc ccatcttcgt cgccggcccg
     1381 atcccgcagc tcgtcaccac caccgagcca gaggagctgc ggctcggacc cacgagtcca
     1441 gctgctgccc gcgtcgtcga ggtccgcatt ggcgcggacg cgatcaccgg cgatgggagc
     1501 gccgtgggca cgatgtccgt ggacctgtcc tcccggccgg gcgccagcta cgactccgag
     1561 catcgcccga tcgtccacgt ctacccgctc ctcggcttcg acgagccgcc gggtaccggc
     1621 ttcctgccgg atcctgccga cgatggcgtg gatccgttcg ccgattgcga acccggcgtc
     1681 gactgcgtcc ggcggttcct cgtgacgttt gcctggaatg gcgtcgacgg gcaggacgcg
     1741 ctgtacgact ggaacctcag ggtgcgccgg ctggacctga tccgggcatg gtcgaccccg
     1801 gtcgagctgt cggcggccat cgtccgtcgt atcgatgtcg cccccggttc ggcgtcgaag
     1861 gtcgtccacc tgggaggtga tgagcagacc acgccggggg gcagtgagcc cgagccgcag
     1921 atccgggtcg gactcgaagg gtcgaccccc gcggaggatc gattggcccg gctcctgcca
     1981 gtgcccgcgg tgatgacgta ccgcgtgcac gtcctcagaa acgatccggc agcgacccag
     2041 gatccgcgcg agttctccaa cgtccagctg cccggcgagc gcatcggcgc ccacgtccac
     2101 ctcgacttct tccgtggcga ggacagcgtg gtcaccaatc cgcttgccgc ctgtcggatc
     2161 ggcgacgtgt gtgacgacat gacgatctcg atcattcggg tggggccgct cgacaaggac
     2221 ccgctgccac cggtcacggc gcactggtcg ctcgacatcc tgacgtactc ctacgtcgac
     2281 atcccggtcg agctcagcgt cgatcgacgt ccttgaggaa gccgagcagg ccggcgaagt
     2341 agggctccgg gtcgtcgtac atcgtcatgt ggctgccgtt ttcgcagagc aggaacgatc
     2401 cccgcgggag ccggcccgac atcatccgca tgaactcggg gtccatcgtg tcgtgctggg
     2461 cggcgatgac cagcgtcggg accatgatcc gctcgaggtc atccgtgcga tcccaggtga
     2521 ggagcacgcc gccggcgccg agctcgctcg ggccctggat cgggacgtag atcgaggggt
     2581 tgatcgcggc gaaagcgcga ttgaccgcgt cgggccactc ggctgccggc ttacgcagga
     2641 cgtggtgttc gtagtggtgg gggatcagca ggcccatgta tcgcgg
//
LOCUS       DATBSW010000247         6512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1117131, whole genome shotgun sequence.
ACCESSION   DATBSW010000247 DATBSW010000000
VERSION     DATBSW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117131"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..662
                     /locus_tag="VIF63_06835"
     CDS             144..662
                     /locus_tag="VIF63_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8239133.1"
                     /translation="MTFVHRFWRPGLVAGALLVALAAIGPVLAATKAVEIVDKTFTPA
                     EITIAPGDTVTWTVTKAIGEPHTVTATATGGAKPAFDSSTGDPDLTKLKENGGTFSFT
                     FTEAGTYPYICTIHAADMKGTIIVIAGGGTPEPAHEGISPERKLLGAGILAVTLIALF
                     GAAWFYRRMNPA"
     gene            complement(688..1275)
                     /locus_tag="VIF63_06840"
     CDS             complement(688..1275)
                     /locus_tag="VIF63_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8239134.1"
                     /translation="MATSASIDDVPSRSTRPRTVRAIARRFEADLGASVLASGPRIPN
                     VRQISERVAMGRALWRELVVGFAYQLGELRLGFTQLAALYVLADGSTTTVGELAEALG
                     RSPSATSRLVDGLAKRRLVERHEEPEDRRQIALTLTQRGQAILRAVDRARADQFLTAV
                     RPMPTPERALVAMGVAALATHAISRRGRLIKSSAR"
     gene            1417..2382
                     /gene="fabD"
                     /locus_tag="VIF63_06845"
     CDS             1417..2382
                     /gene="fabD"
                     /locus_tag="VIF63_06845"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8239135.1"
                     /translation="MSEIAFVFPGQGSQAVGMGRALAATSPAAAAVFADADAALGEGI
                     SALAWAGPEDQLNRTENAQPALLATSIAYLAAARERASAQGMRLPEPRFFAGHSMGQY
                     SAMVAAGSLALGDGVRLVRERGVQMQASGAGRDGAMAAVLGLDDAAMPELVKGVSAHG
                     VFAVANRNSPGQVVVSGERAAIDAAAELAKALGAKRAIVLPVSVAAHSPLMAEAATAM
                     REILAKVDFSDPSAPLLANADARAITTAAGARAELVEHLTAGVDWVGAIQAMTAAGVD
                     TFVEVGPGKVLTNLIKRIAPEATAIALEDALAAEPLELPILIPVS"
     gene            2392..3666
                     /locus_tag="VIF63_06850"
     CDS             2392..3666
                     /locus_tag="VIF63_06850"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8239136.1"
                     /translation="MRSPDHTRRVVVTGLGVVSSVGNDKDTAWNSLVEGRSGLAELTR
                     FDISRYEHKAGGEVKDFDASAWMNPKAVRRSEREMHYGVAAAKQALTDSGFEIDDSNR
                     TDVGVVFGSGAGGQQLMIENFSTLIEKGPDRVQPTFIANALVDSTSGMIAIETGAIGH
                     NLSIVSACSTGTHNVGEAAEAIRRGDCIAIISGSTEAPLLEVAHAGFTNMRGMGSPRP
                     GESIQSVSRPFDATRNGFVLGEGAGSLFLEDLELAKKRGAKIYAEVIGYGSAADGWDM
                     IQPIEGGIGSARAMKMALDRRGVPADEVDMINPHGTSTPVGDAREAEAIWAVFGDRSA
                     RDRRSLAISGTKSMTGHMMGAAGAFEAFATVMSVAEQTVPGTLNYRDFDPACDLWVAD
                     ETIKMPVRYALSNNIGLGGHNGAVIFKRYDGD"
     gene            3674..4939
                     /gene="fabF"
                     /locus_tag="VIF63_06855"
     CDS             3674..4939
                     /gene="fabF"
                     /locus_tag="VIF63_06855"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8239137.1"
                     /translation="MPSQDPARRAVVTGWGAISPIGNDAETYWSNLLAGVPGGGPITS
                     FDTTGHDVKIAAEVKGFDPLLTMDRKMVRRMSRFIHFGVAAATEAVRHSGLEFEALSP
                     DQRDRAAVVVNTGGGGMEQVINGADTIRDKGPGQVSPFAIPALSGSMAAAQVSMNYGI
                     TGPVITQVAACASGVIAYQDALRLIGSGECDIVIAGGSEAPLLAMAFAALANMTALSK
                     RNDDPEGASRPFDRTRDGFVFGEGAGIMVIESAAHAMARGATIMAEIVGASLTADAYH
                     ISAPEPTGRGASMAMTKSLRQAGLAPDDIGHIVAHGTSTPLNDVTETRAIKAAFGEHA
                     YRVPVSSPKSMIGHTLGAAGALSAIAAVGAIRDGWVAPTINYREPDPDCDLDYVPNVK
                     RQVKVDAAMINGFGFGGQNAVAIFKRFEA"
     gene            complement(4936..5430)
                     /locus_tag="VIF63_06860"
     CDS             complement(4936..5430)
                     /locus_tag="VIF63_06860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8239138.1"
                     /translation="MPESRSRIELPVDPRDIDGDFAHRHEVEVRLSDTDAMGHVNNAR
                     YLTYVEIARVAYYERVTGNLLPIGTHGAEEGMILAEIRMTYRSPAFYGETLTVESRVE
                     RIGRTSFAMVHRITAPQSRVAAARLIAVADSTLVAYDYQAEQPIPVPNEWRAAMEAFE
                     GRSF"
     gene            complement(5451..>6512)
                     /locus_tag="VIF63_06865"
     CDS             complement(5451..>6512)
                     /locus_tag="VIF63_06865"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239139.1"
                     /translation="FVLGVAAAARSIPVLILVVPAGIAADRFDRRKIVVTTSIVSTVA
                     SGLLAILTIAGTIDITMVLIIAIVLGMSNAFEMPARQSYVSELIGKRDLANAIALNSL
                     LFNGARVVGPAVAGILVALVGPGWAFGINALSFIPVIIGLLMISRAYVPRAAFAVGSA
                     VPEAIRYLRGEPRVAALLALLAAQTVFASGQFILGPALAQDLGQGAEGLGVLLSAAGA
                     GAVVGGLRLAATAHRDERWRVLLLAGVALSVGLLGIGLTSSYPVTLLCLVVTGWGTVT
                     FNASSNTLIQTIVPDRLRGRMMSMYTLVLLGLMPVGGILMGALATQFGSASAIAMGGV
                     AYGAIVLAAFGGSRSLRSL"
BASE COUNT         1031 a         2270 c         2291 g          920 t
ORIGIN      
        1 ccgacctacg ggttctcgcc gctgcgccag ccgcccggtg agacgtacct tgaccgctac
       61 cacggggtcg acgagcgggt ctcgctcgag ggcctccgct ggggcctgcc cgtcctctac
      121 gatgccgttc ggaggttctg cggatgacgt tcgtgcaccg gttctggcgc cccggcctcg
      181 ttgcgggcgc gctcctcgtc gcgcttgccg ccatcggacc ggtcctcgcg gcgacgaagg
      241 cggtcgagat cgtggacaag acgttcacgc ctgcggagat cacgatcgcc ccgggcgaca
      301 ccgtgacgtg gaccgtgacg aaggcgatcg gcgagccaca tacggtgacc gcgacggcga
      361 ccggcggcgc gaagccggcg ttcgattcga gcacgggcga ccccgacctc accaagctca
      421 aggagaacgg cggcacgttc tcattcacgt tcaccgaggc gggcacgtac ccctacatct
      481 gcaccatcca cgccgccgac atgaagggca cgatcatcgt catcgccggc ggcggcacgc
      541 ccgagccggc gcacgaggga atctcgccgg agcgcaagct ccttggcgcg ggcatcctcg
      601 cggtcaccct gatcgcgctg ttcggcgccg cctggttcta tcggcggatg aaccccgcct
      661 gacgcggcgc ggtgacgagc gcgccgacta gcgcgccgag ctcttgatca ggcggccacg
      721 gcgcgagatc gcgtgcgttg ccagtgcggc gacgcccatc gcgaccagcg cacgctccgg
      781 ggtgggcatg gggcggaccg cggtcaggaa ctggtctgcc cgagcccgat cgaccgcgcg
      841 caggatcgcc tgcccgcgct gggtgagcgt cagcgcgatc tggcgccggt cctcgggttc
      901 ctcgtgacgc tcgaccaggc gccgcttggc gagaccgtcg accagcctgc tcgttgccga
      961 cggcgagcga cccagcgcct cggcgagctc gccgaccgtg gtcgtgctgc cgtccgcgag
     1021 gacgtacagc gccgccagct gggtgaagcc cagccgcagc tcgccgagct ggtacgcgaa
     1081 accgacgacc agctcgcgcc acagcgcccg gcccatcgcg acgcgctcgg agatctggcg
     1141 gacgttgggg atgcgcggtc cggaggccaa cacggaagcg cccaggtcgg cttcgaagcg
     1201 gcgggcaatg gcgcggacgg tacgcggccg ggtggacctc gacggcacgt cgtcgatgct
     1261 agcgctggtc gccatcgcgc gcaatcgtgt gcaatcgcgc acacgtccca cgctcgcgcg
     1321 tggcatgatc cggcagagtt tcggtgttcg cggtcaacgg cgactcgctt cgccgcgccc
     1381 agccgcgcca atcaccagca acaacctcgg gagctcatga gcgagatcgc tttcgtcttc
     1441 cccggccagg gctcgcaggc cgtcggcatg ggtcgcgccc tcgcggccac ctcgccggcc
     1501 gcggccgcgg tcttcgccga cgccgacgcc gcgctcggcg agggcatctc ggcgctcgcc
     1561 tgggccgggc ccgaggacca gctcaaccga accgagaacg cgcagccggc gctgctcgcg
     1621 acctcgatcg cctacctcgc cgccgcgcgc gagcgcgcat cggcgcaggg catgcgcctg
     1681 ccggagccgc gtttcttcgc cggccattcg atgggccagt actccgcgat ggttgctgcc
     1741 ggcagcctcg cgctcggcga cggcgtccgg ctcgtgcgcg agcgcggcgt ccagatgcag
     1801 gcgagcggtg caggccgcga tggcgcgatg gccgcggtcc tcggcctgga tgacgcggcc
     1861 atgccggagc tcgtgaaggg cgtatcggcc catggggtct tcgccgtcgc gaaccgcaac
     1921 tcgcccggcc aggtcgtggt gtccggcgag cgcgcggcga tcgacgcggc tgccgagctc
     1981 gcgaaggccc tcggcgccaa gcgcgccatc gtcctgccgg tgtccgtcgc cgcccattcg
     2041 ccactcatgg ccgaggcggc aaccgcaatg cgcgagatcc tcgccaaggt cgacttcagt
     2101 gacccgagcg cgccgctcct cgccaacgcg gacgcccgcg ccattacgac ggcagccggc
     2161 gcgcgcgccg agctcgtcga gcacctgacc gccggcgtcg actgggtcgg cgcgatccag
     2221 gccatgaccg cggccggcgt tgatacgttc gtcgaggtcg gaccgggcaa ggtcctgacg
     2281 aacctcatca agcgcattgc ccccgaggcg accgccatcg ccctcgagga cgcgctcgct
     2341 gccgagccgc tcgaactgcc catcctcatc ccggtctcat aggagtcact cgtgcgcagc
     2401 ccagatcaca ctcgtcgcgt cgtcgtcacc ggcctcggcg tcgtgagctc ggtcggcaat
     2461 gacaaggaca ccgcctggaa cagcctcgtc gaggggcggt ccggcctggc cgagctcacc
     2521 cgcttcgaca tctcgaggta cgagcacaag gccggcggcg aggtcaagga cttcgatgcc
     2581 tcggcatgga tgaatcccaa ggccgtgcgc cgcagcgagc gcgagatgca ctacggcgtc
     2641 gcggcggcga agcaggcgct cacggactcc ggcttcgaga tcgacgacag caatcgaacc
     2701 gacgtcggcg tggtcttcgg ctcgggcgcg ggtggccagc agctgatgat cgagaacttc
     2761 tcgacgctca tcgaaaaggg cccggatcgc gtccagccga cgttcatcgc gaacgccctc
     2821 gtcgactcga cgtcgggcat gatcgcgatc gagacgggcg cgatcgggca caacctgagc
     2881 atcgtctcgg cctgctccac cggaacgcac aacgttggcg aggcggccga ggcgatccgc
     2941 cggggcgact gcatcgcgat catctcgggc tcgaccgagg cgccgctcct cgaggttgcc
     3001 cacgccggct tcaccaacat gcgcggcatg ggctcgccgc gcccgggcga gtcgatccag
     3061 tcggtctcgc gcccgttcga tgcgacgcgc aacggcttcg tgctcggtga gggcgcgggt
     3121 tcgctgttcc tcgaggacct cgagctggcg aagaagcgcg gcgcgaagat ctacgccgag
     3181 gtcatcggct acggctccgc ggcggacggc tgggacatga tccagccgat cgagggcggg
     3241 atcggctcgg cccgagcgat gaagatggcg ctcgaccgac gcggcgtgcc ggcggacgag
     3301 gtcgacatga tcaaccccca cggcacgtcg actcccgtcg gcgatgcccg cgaggcggag
     3361 gcgatctggg cggtcttcgg tgaccgctcg gcgcgcgacc ggcgttcgct ggcgatctcg
     3421 ggcacgaagt cgatgaccgg ccacatgatg ggcgcggccg gcgcgttcga ggcgttcgcg
     3481 accgtcatgt ccgtggccga gcagaccgtg cccggcacgc tcaactaccg cgacttcgat
     3541 ccagcctgcg acctgtgggt cgccgacgag acgatcaaga tgccggtgcg gtacgcgctc
     3601 tcgaataaca tcgggctcgg cggacacaat ggagcagtga tcttcaaacg ctacgacgga
     3661 gactgaccca ccgatgccca gccaggaccc agcgcgccgc gccgtcgtga ccggctgggg
     3721 cgccatctcg cccattggca acgacgccga gacgtactgg tcgaacctcc tcgccggcgt
     3781 gcccggcggc ggcccgatca cgagcttcga cacgaccggg catgacgtga agatcgcggc
     3841 cgaggtcaag ggcttcgatc cgctcctgac gatggatcgc aagatggtcc ggcgcatgag
     3901 ccggttcatc catttcggcg ttgcggcggc gacggaagca gtgcgccact ccgggctcga
     3961 gttcgaggcg ctgtctcccg accagcggga ccgtgccgcg gtggtcgtca acaccggcgg
     4021 tggcggcatg gaacaggtca tcaatggcgc ggacacgatc cgtgacaagg gccccggcca
     4081 agtgagcccg ttcgcgatcc ccgccctctc gggcagcatg gccgcggcgc aggtttcgat
     4141 gaattacggc atcaccgggc cggtcatcac ccaggtggcg gcgtgtgcgt cgggcgtgat
     4201 cgcgtatcag gacgccctgc gcctcatcgg gtccggtgaa tgcgacatcg tcatcgccgg
     4261 cggctccgag gcacccctgc tggcgatggc gtttgccgcg ctcgcgaaca tgacggcgct
     4321 gtcgaagcgc aacgacgacc ccgagggcgc gtcgcggccg ttcgatcgga cccgcgatgg
     4381 cttcgtcttc ggcgagggcg ccggcatcat ggtcatcgag tcggcggcgc atgcgatggc
     4441 tcgcggcgcg acgatcatgg ccgagatcgt cggggcgtcg ctgaccgccg acgcgtacca
     4501 catctctgct cccgaaccga ccggccgggg cgcctcgatg gcgatgacca agtcgctgcg
     4561 ccaggcgggc ctcgcaccag acgacatcgg ccacatcgtc gcccacggca cctccacccc
     4621 gttgaacgac gtcaccgaga cacgcgccat caaggcagcg ttcggcgagc acgcgtacag
     4681 ggtgccggtg tcatccccga agtcgatgat cggccacacc ctcggcgccg ccggggcgct
     4741 gtcggcgatc gcggcggtcg gcgcgattcg cgacggctgg gtcgctccga cgatcaacta
     4801 tcgcgagccc gacccggact gtgacctcga ctacgtcccg aacgtgaagc gtcaggtcaa
     4861 ggtcgatgcc gcgatgatca atggcttcgg tttcggcggc cagaacgccg tcgcgatctt
     4921 caagcgcttc gaggcctaga agctccgacc ctcgaacgcc tccattgcgg cgcgccactc
     4981 gttgggaacc gggattggtt gctcggcctg gtagtcgtag gcgaccagcg tcgaatccgc
     5041 gacggcgatg aggcgcgcgg cggcgacccg gctctgcggc gccgtgatcc ggtggaccat
     5101 cgcgaagctc gttcggccga tgcgctcgac ccggctttcg accgtcagcg tctcgccgta
     5161 gaacgccggg ctgcgatacg tcatccggat ctcggcgagg atcatcccct cctcggcacc
     5221 gtgggtgccg atcggcagca ggttgccggt cacccgctcg tagtaggcga cgcgggcgat
     5281 ctcgacatag gtcaggtagc gggcattgtt gacgtggccc atggcgtcgg tgtcggacag
     5341 gcggacctcg acctcgtggc gatgggcgaa atcgccgtcg atgtcgcgcg ggtcgacggg
     5401 gagctcgatg cgggagcggg attcgggcat gggcggaatc taggccgcgc tcagaggctg
     5461 cgcagcgacc gggacccgcc gaacgcggcg agcacgatcg cgccgtaggc cacgccgccc
     5521 atggcgatcg ccgaggccga gccgaactgg gtcgccagcg cgcccatcag gatcccgccg
     5581 acgggcatga gcccgaggag caccagcgtg tacatgctca tcatccggcc gcggagccgg
     5641 tctggaacga tggtctggat gagggtgttc gacgaggcgt tgaaggtgac ggtgccccag
     5701 ccggtgacca cgaggcagag cagcgtcacc ggataggagc tcgtcaagcc gatgcccagg
     5761 agccccacgg agagcgccac cccggcaagg agcaggactc gccatcgctc gtcacggtgg
     5821 gccgtcgcgg caagtcggag cccgccgacc accgctccgg cgccggccgc ggacaggagc
     5881 acgccgaggc cctcggcgcc ctgcccgagg tcctgcgcga gcgccggccc caggatgaac
     5941 tgaccggacg cgaagacggt ttgggccgcc agcaacgcca gcagtgcggc cacccgcggc
     6001 tcgccgcgga ggtagcggat tgcctcgggc acggccgaac cgacagcaaa ggcggcacgg
     6061 ggcacatagg cgcggctgat catcagcagg ccgatgatca cggggatgaa gctgagggcg
     6121 ttgatcccga atgcccagcc cggcccgacg agggcgacga ggatgccggc gaccgccggc
     6181 ccgacgaccc gggcaccgtt gaacaggagc gagttcaagg cgatggcgtt ggcgaggtcg
     6241 cgcttgccga tgagctcgga cacgtagctc tgccgggccg gcatctcgaa ggcgttggac
     6301 atgccgagga cgatcgcgat gatcaggacc atggtgatgt cgatcgtgcc ggcgatggtc
     6361 aggatcgcca gcaggccgga cgcaaccgtc gagacgatgc tcgtcgtgac gacgatcttc
     6421 cgccggtcga accgatccgc ggcgatcccc gcgggaacga cgaggatcag cacggggatg
     6481 gagcgcgcgg cggcggcgac gccgagcacg aa
//
LOCUS       DATBSW010000248         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_940997, whole genome shotgun sequence.
ACCESSION   DATBSW010000248 DATBSW010000000
VERSION     DATBSW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940997"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VIF63_06870"
     CDS             <1..919
                     /locus_tag="VIF63_06870"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY8239140.1"
                     /translation="PLTGLPVSPEAALHHPIAVMIDDDSHARPQGGFNAASIVWHAPA
                     EGGVPRYMLIFQDQIPTAVGPIRSSRQYYIEWAAEWAALYVHHGGSPQALATLAAKGH
                     GQWVWNADGFRWEGRFVFRAKDRRAPHNVMTDGEHLRKLALKVGAVDEPITPVWTFGP
                     ALRRAERPAGTTIVVTYPYETITYRYSPVTNRYVRYLNKSKTPQVDVADGHVVAPTNV
                     VILRMFFGPLNDGHPSKHRLEARNIGKGEAWISTNGVTVKGTWSKKSATAPTLLFGPD
                     GAPVTLTAGQTFVQVIPLTYSFKITDGTK"
     gene            complement(953..1717)
                     /locus_tag="VIF63_06875"
     CDS             complement(953..1717)
                     /locus_tag="VIF63_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY8239141.1"
                     /translation="MTESAMPLLGTGTWVVLPTYDEAENLPGIAQAILAALPGATLLV
                     VDDGSPDGTGRLADGLAADNLRVRVRHRPGKAGLGKAYLEGFQVALEGGATSLVQMDA
                     DWSHDPLVLPALLEPITSDRADLVIGSRYVPGGRVLDWGLGRRLISRLGSIFAKTVLR
                     LRPHDLTGGFKAWRASTLAAIPFDGIHAGGYVFQIEMTHRASRAGARVTEVPITFQDR
                     RVGRSKMSRRIIVEALVVVVRLRWDELRKRRIRRRS"
     gene            complement(1807..>2740)
                     /locus_tag="VIF63_06880"
     CDS             complement(1807..>2740)
                     /locus_tag="VIF63_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY8239142.1"
                     /translation="AHSLTWKLTFGGPIAGLRSIDFSRSGTATGCTIGTPVGSGASWS
                     VTLTGCSAGTVVLGLKPETVVDAVRNWGPRSQVNASSLLIDRTAPHAATPQVSLRSGV
                     TLPSTSTSAGLMATLDLAATDPGGAGVRDYDVRRSTDGGAWSTVGVDLTAATLNLTLS
                     PGHAYRFSVRARDRAGNVGDWVTGPVIRAYLSQQTNTAINWKGSWTRVIDPRFSGGSV
                     RYATAAGASATYAFTGRAVAWVTTVAPNRGAARVYLDGVLVATVNTYAATTTFGRVAF
                     AKAWASSGFHTIRIVSAGTVGHGRIDIDALEVVR"
BASE COUNT          411 a          962 c          991 g          376 t
ORIGIN      
        1 gcccctgacc gggctgcccg tgagccccga ggccgcgctc catcacccga tcgccgtgat
       61 gatcgatgac gactcgcacg cccggccgca gggcgggttc aacgcggcgt cgatcgtctg
      121 gcatgcgccc gcggaaggcg gcgtgccgcg ctacatgctg atcttccagg accagatccc
      181 gacggcggtc ggcccgatcc ggagctcgcg ccagtactac atcgagtggg ccgcggagtg
      241 ggccgccctc tacgtgcatc acggcggctc gccgcaggcg ctcgcgaccc tggccgcgaa
      301 gggccacggc cagtgggtct ggaacgcgga cgggttccgc tgggagggtc gcttcgtctt
      361 ccgggcgaag gaccggcgtg cacctcacaa cgtcatgacc gacggcgagc acctgcgcaa
      421 gctggcgctc aaggtcggcg cggtcgacga gccgatcacg cccgtctgga cgttcggccc
      481 ggcgctgcgg cgagccgagc gcccggcggg cacgaccatc gtcgtgacct acccgtacga
      541 gacgatcacc taccggtact ccccggtcac gaaccgctac gtgcgctacc tgaacaagtc
      601 gaagacgccc caggttgacg tcgccgacgg gcacgtcgtc gccccgacga acgtggtgat
      661 cctgcggatg ttcttcggcc ccctgaacga cgggcacccg agcaagcatc gcctggaggc
      721 gcgcaacatc ggcaagggcg aggcgtggat ctcgaccaac ggcgtcacgg tcaaaggcac
      781 ctggtcgaag aagtcggcga cggcgccgac gctcctgttc ggcccggacg gcgcgccagt
      841 cacgctcaca gccggccaga cgttcgtgca ggtgatcccg ctgacctact cattcaagat
      901 cacggacggg accaagtagg tacacgtccc gccctaacgc tggcgaggtc gcctagctcc
      961 tgcggcggat ccggcgcttg cgcagctcgt cccagcgcag gcggacgacc acgacgagcg
     1021 cctcgacgat gatccggcgg gacatcttcg agcggccgac gcgccggtcc tggaacgtga
     1081 tcgggacctc ggtgacgcgg gcgccggcgc ggctcgcccg gtgggtcatc tcgatctgga
     1141 acacatagcc gccggcgtgg atgccgtcga acgggatcgc ggcgagggtc gaggcgcgcc
     1201 acgccttgaa gccgcctgtc aggtcgtgcg ggcgaagtcg gagcacggtc ttggcgaaga
     1261 tgctgcccag ccgcgagatg agccgccggc cgagccccca gtcgagtacg cgcccgcccg
     1321 ggacgtagcg gctgccgatg accaggtcgg cgcgatcgct cgtgatgggc tcgagcaggg
     1381 ccggcagcac cagcggatcg tgcgaccagt cggcgtccat ctggacgagg gacgtggcgc
     1441 cgccctcgag cgcgacctgg aagccctcga ggtatgcctt gccgaggccg gccttgcccg
     1501 ggcggtgccg aaccctgacc cgcaagttgt cggcagcgag gccatcggcg agacggccgg
     1561 tgccgtcggg cgagccgtcg tcaacgacga ggagcgtcgc gcccgggagg gcggcgagga
     1621 tcgcctgggc gatgcccggc aggttctcgg cctcgtcgta cgtcgggagc acgacccagg
     1681 tgcccgtgcc gagcaggggc atagcggatt cggtcatggc cgaggagtgt aggggccggg
     1741 tcgcgcgagc ccgcccggcc cgcggccgtg cagtccgtgc tcgttcggag cgcggcggcg
     1801 tcgccgtcat cggacgacct cgagggcatc gatatcgatg cgcccgtgcc cgaccgtgcc
     1861 ggcactgacg atccggatcg tgtggaaccc gctgctggcc caagccttcg cgaacgcgac
     1921 gcgcccgaat gtcgtcgtcg cggcgtaggt gttgacggtc gcaaccagca cgccgtcgag
     1981 gtagacccgg gcggccccgc ggttgggcgc aacggtcgtg acccaggcaa ccgcacggcc
     2041 cgtgaacgcg tacgtcgcac tcgccccggc ggccgtcgcg tagcgcaccg agccgcccga
     2101 gaagcgcggg tcgatgaccc tcgtccagct gcccttccag ttgatcgcag tgttggtctg
     2161 ctgggacagg taggcgcgga tcaccgggcc tgtgacccag tcgccgacat tgccggcgcg
     2221 gtcccgtgcg cggaccgaga agcggtaggc gtggccaggg ctcagggtga ggttgagggt
     2281 cgctgcggtc aggtcgacgc cgacggtcga ccaggcgccg ccgtccgtgc tgcgacgcac
     2341 gtcgtagtcg cgaacgcctg ccccaccggg gtccgtcgcg gcgaggtcga gcgtcgccat
     2401 gagcccggcc gacgtggacg tcgatggcag cgtcacgccc gaccgaagcg agacctgtgg
     2461 cgtcgcggcg tggggcgccg tccggtcgat cagcaggctg gatgcgttga cctggctccg
     2521 gggcccccag ttgcgcaccg catcgacgac cgtctccggc ttgagcccga gcacgacggt
     2581 acccgccgag cagccggtaa gggtcacgct ccagctggcc cccgacccga ccggcgtgcc
     2641 gatcgtgcag cccgtggccg tcccgctccg gctgaaatcg atgctccgga ggccggcgat
     2701 cggcccgccg aacgtcagct tccacgtcag gctgtgggcc
//
LOCUS       DATBSW010000249         8715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_694050, whole genome shotgun sequence.
ACCESSION   DATBSW010000249 DATBSW010000000
VERSION     DATBSW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694050"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VIF63_06885"
     CDS             complement(<1..482)
                     /locus_tag="VIF63_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8239143.1"
                     /translation="MGRRASWIAFASTLATFGLLGWVVVNAPNWPAVQRSFLSGEEFT
                     DALPAVVWALRINVQLFLFAEVLILIVGLGIAVLRSLPGAVFFPVRLLAIAYVDIFRA
                     LPGVLVIFFLGLGVPSLRLPGGPKEPFFWGVIALTLIYSAYVSEVYRAGIESIHPSQD
                     A"
     gene            complement(505..1431)
                     /locus_tag="VIF63_06890"
     CDS             complement(505..1431)
                     /locus_tag="VIF63_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8239144.1"
                     /translation="MGRIRFATFGVIALLVAACSGGEGASTAPSQAASTTTAATPAPT
                     EDACAAANLDTVSPGLLTIATSEPAFPPYFAIDDPIPDGSIWTLGQPPNGMGLESATA
                     YGVAQKLGFSNDQVNWVIGDFNTVTQPGDKDFDLFIAQVSYSAERAGVVDLSDGYFDN
                     NQAVVAVKGSDITKVTTVAGLKPFKFGAQVGTTSLAYITDQIKPTKEPSVYDSNDAAV
                     QALNAGQIDAIVADLPTTFYMRDAQLDNGQIVGSLPSVPGVEVEHFSILLDKGSALTP
                     CVNAALAAMKEDGTLDGIVEAWITSQGAPELK"
     gene            complement(1464..2285)
                     /locus_tag="VIF63_06895"
     CDS             complement(1464..2285)
                     /locus_tag="VIF63_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY8239145.1"
                     /translation="MGERPVIGFLTDFGLDGAAAICRGVMLSIARDAQIVDISHTVAK
                     YAIADGAYLLRSAVPWLPIGVHLAVVDPGVGTDRRPIGLLTARGDALIGPDNGLLVPA
                     ADRLGGIVAARLLSNPDWLLPATSATFHGRDIFAPVAAHLALGESFADAGAPFDPGSL
                     VRLDLPSVTVESGRLDTGVTYVDSFGNVRLAGGAADLAEALGPVEAGAALSVEVGPTQ
                     STVEARWRRTFGDAPAGELLVYEDSSGNLALAVSSGDAARTLGASTGARIRIRRG"
     gene            complement(2419..2718)
                     /locus_tag="VIF63_06900"
     CDS             complement(2419..2718)
                     /locus_tag="VIF63_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239146.1"
                     /translation="MEPGIEASLKATYRALLARGLEAGQSANLTAFLHGLPSHGFQWT
                     LPEIEAILRRRFAHVAEVRAAARTAPASDHHRDSAIPNRRRTRSGSTHTIDLPKP"
     gene            2867..3871
                     /locus_tag="VIF63_06905"
     CDS             2867..3871
                     /locus_tag="VIF63_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY8239147.1"
                     /translation="MDDLDALEHEPAKRDRTARLMRVAGLLAGNPDGMRPLDIAERLG
                     MSVRNVYRDLRALESEVKLPVWSEGGRWGILGGAFLPPLKLSLSEAMAVFLGARLMVR
                     YADKYDPDLASAFEKLAATLPEALREHVARSLDVLQDAPRDQDFIDHVRLLTRAWAER
                     RVVEITYEGAAFEGKVGERSRRAIRPYLIEPSLQTHALYLIGWDEDKGGMRTFKIERI
                     REVRVTQRTFDPPEEATLERDLRRGWDIIADQPAIEVIVRFSTAVADRVLETTWHSLQ
                     RTERTPDGGLVWRSTVSGVIEIRLWILSWGDDAEVLEPAELRTQVRDILGRAAAQYEM
                     "
     gene            4017..4832
                     /locus_tag="VIF63_06910"
     CDS             4017..4832
                     /locus_tag="VIF63_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8239148.1"
                     /translation="MARRRPRAATLARLVMVALFASLALTTAGPLANPSIVAASTADT
                     MEADLLKWVNNARAQRGLKPLRLHSGLVDLAGDQAAKMASSGSMQHISCMSCTFNNRG
                     IQYYSVGENIAYTTYPWGDLAVASIYNGWKGSSMHWNLLMSSKFNYVGFGVAYRSSSH
                     KTFAAAELSESKDRTDPSPRTGAVSRSGTTVSWSWSGADVALQTHTAGLKNYDVEYRV
                     GSGSWSTIKSATTTTSLSLSGRATGHWYGLRIRARDNAGNVSGYSAEMKVWVP"
     gene            4881..6275
                     /locus_tag="VIF63_06915"
     CDS             4881..6275
                     /locus_tag="VIF63_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8239149.1"
                     /translation="MARHPFAAVNLISDPIHGYLELTKRLARDESAAAGLPDEDAAED
                     DLLDTAWLQRLRRISQLQSARWVFPTAEHSRFTHGLGVMHEAGLWARTLLPSLRTVLP
                     TLAPTEPLPSEGLVVETLRVAGLLHDVGHGPFAHFFDDRVLAAFPAPEDARRGAGKRL
                     SHEDLSQLIIERELGSVIRALRRAPGATPERDAFADDETIDPRWVSFLVSKPALIDAS
                     MPAWVRLLQPLLSGVFTVDNLDYVRRDAFFTGVRVGIDVERLRRYTFVSERGLTLYEP
                     GVAALEGFLGARMLLHQAVYFHRTVRAIDLDLGEVFQPAIAALFGDDSPADRLSDYVD
                     LDEYALLHQAARWARGQDVLADGARGEPGGLRVTRAVADGWRGILLRRPGWRAEAEIR
                     AAYEAGLEPEAEIASLGQAVPGSVAIDLAVVDARPEGRTLAIEGRDGSDIPVNEALAR
                     VPAFAVVGRRYRRL"
     gene            complement(6315..7283)
                     /locus_tag="VIF63_06920"
     CDS             complement(6315..7283)
                     /locus_tag="VIF63_06920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8239150.1"
                     /translation="MTPRATRCAPRADAIDTRPYPRAPRRFAHAHPAVKRRRGAIALA
                     LAAALIATSWAWLGLFPGHDTLARLSPVTRTATATREAANVPTHGPQGDDVGGGTVAP
                     GNLAAVPAELGFDRSVWLERRGVAPEPPKGYRWPLRNATITNDFGRGLDGSFVLRGKS
                     FHDGLDIATWCGDRIRAAHDGVVLAAGRKHQKYMGWIGDLTPFKEKMDRVGWGGQSIT
                     VVIDDRNGYRSGYSHLSRAIVKIGQVVRAGDLIGYEGETGNATGCHLHYTLFNPNETE
                     TLRLNPRAVELTKLPGLIVARINPFEVLPPMDSANMRWGWDARIRH"
     gene            complement(7246..7656)
                     /locus_tag="VIF63_06925"
     CDS             complement(7246..7656)
                     /locus_tag="VIF63_06925"
                     /inference="COORDINATES: protein motif:HMM:NF045421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239151.1"
                     /translation="MATGSGTKADPWVLKTPPGTSEYTMFIDDAADPPVIVCQVGSTT
                     LKYDKRAIDDLHAALKAHGDWMDLGAADEQKDAKPGTVEAWGRDPSNPVGGWYGLRKG
                     YRGRFGMYMPPLMEALGLAELTHDAKGNKMRAKG"
     gene            7790..>8715
                     /locus_tag="VIF63_06930"
     CDS             7790..>8715
                     /locus_tag="VIF63_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8239152.1"
                     /translation="MRVVVVGAGFAGLAAALELHDRGVEVVVLEARDRVGGRVWSREL
                     PSGAVIEMGAEFILPGDATIRAVTDRLGLQLFAKGTSYGDREPRGGQPVTREELEAGY
                     AAVAAAAREGRLGSGSVVGALRELPMTEGAREAIRARVEVSTAYPADDQAAAVLSEGG
                     TGTGDFATHSVAGGNQRIALAIAERLRGAVHLRAEVVYVAWALAGSVDGPAAAPAGVR
                     VRAGGDVIEAEAAVIAVPASLIDRIAFDPPLPTWKAAALAAVRYGHAAKLFLPLDELA
                     PPSATLSVPDRFWTFTQHAQDGSPLPVAASFVG"
BASE COUNT         1250 a         3025 c         3124 g         1316 t
ORIGIN      
        1 gcgtcctggc tgggatggat cgactcgatg ccggcccggt agacctcgga cacgtatgcc
       61 gagtagatca acgtcagggc gatgacgccc cagaagaagg gctccttcgg acccccgggg
      121 agtcgcaggc tcggcacacc gaggcccagg aagaagatca caaggacgcc gggcagggcg
      181 cggaagatgt ccacgtaggc gatcgccagg agccggaccg ggaagaagac cgcaccgggc
      241 aggctgcgca ggaccgcgat cccgagcccc acgatcagga tcaggacctc ggcgaacagg
      301 aacagctgga cgttgatccg gagcgcccag acgaccgcgg gcagcgcgtc ggtgaactcc
      361 tcgccgctga ggaacgagcg ctggaccgcc ggccagttcg gggcgttgac gaccacccag
      421 ccgagcaggc cgaaggtcgc cagcgtgctg gcgaaggcga tccagcttgc gcgccgcccc
      481 atggcggccc ggccgaaggg cgagctactt gagctcgggc gccccctggc tcgtgatcca
      541 tgcctcgacg atgccgtcga gcgtgccgtc ttccttcatc gcggcgaggg ccgcgttcac
      601 gcacggcgtg agggcgctgc ccttgtcgag caggatgctg aagtgctcga cctcgacgcc
      661 gggcaccgac ggcagcgagc cgacgatctg gccgttgtcg agctgggcat cgcgcatgta
      721 gaaggtcgtc ggcaggtccg cgacgatcgc atcgatctgg cccgcgttga gggcctggac
      781 cgcggcgtcg ttcgagtcgt agaccgacgg ctccttggtg ggcttgatct ggtccgtgat
      841 gtaggcaagg ctcgtcgtcc cgacctgggc gccgaacttg aacggcttga ggcccgcgac
      901 cgtcgtgacc ttggtgatgt cgctgccctt gaccgcgacg acggcctggt tgttgtcgaa
      961 gtagccatcg gacaggtcga cgacgccggc gcgctcggcg ctgtacgaca cctgggcgat
     1021 gaacaagtcg aagtccttgt cgcccggctg ggtcacggtg ttgaagtcgc cgatgaccca
     1081 attcacctgg tcgttcgaga agccgagctt ctgcgccacg ccgtaggccg tcgccgactc
     1141 gaggcccatg ccgttcggcg gctggcccag cgtccagatc gacccatcgg ggatcggatc
     1201 gtcgatcgcg aagtacggcg ggaaggccgg ctccgaggtg gcgatcgtga ggaggcccgg
     1261 gctgaccgtg tcgaggttcg ctgccgcgca ggcgtcctcg gtcggggccg gcgtggccgc
     1321 agtcgtcgtg ctcgcggcct ggctcggcgc ggtgctggcg ccctcaccac cgctgcacgc
     1381 cgcgacgagc agggcaatca cgccgaacgt ggcgaaacgg atgcggccca tgggtgtcct
     1441 cctctggtcg gcggagtgta gcgtcagcct cggcggatgc gaattcgcgc tcccgtgctc
     1501 gcgccgagcg tgcgcgcggc gtcaccgctc gacacggcga gcgcgagatt gcccgaggaa
     1561 tcctcgtaga cgagcagctc gcccgcgggc gcgtcgccga aggtgcgccg ccagcgtgcc
     1621 tcgacggtcg actgcgtcgg gccgacctcg acgctcagcg cggctcccgc ctcgaccggc
     1681 ccgagcgctt cggcgagatc cgcagctccg ccggccaggc gcacgttgcc gaacgagtcg
     1741 acgtaggtga ccccggtgtc caggcgcccc gactccaccg tcaccgacgg caggtccagg
     1801 cggaccagcg agcccggatc gaacggcgcg ccggcatccg cgaacgactc gccgagggcg
     1861 aggtgcgccg cgaccggcgc gaagatgtcg cggccgtgga acgtggcgct cgtggccggc
     1921 aggagccagt ccggattcga gaggagccgc gccgccacga tcccgccgag ccggtccgcc
     1981 gcgggcacga gcaggccgtt gtcgggaccg atgagggcgt cgccccgagc cgtcaggagg
     2041 ccgatcgggc gccggtcggt gccgaccccg ggatccacca cggccaggtg aacgccgatc
     2101 ggcagccacg gaacggcgga tcggagcagg tacgccccgt cggcgatcgc gtacttcgcg
     2161 acggtgtggc tgatatcgac gatctgggcg tcgcgggcga tcgacagcat cacgccccgg
     2221 cagatggccg cggcgccatc gagcccgaag tcggtcagga acccgatgac cggccgctca
     2281 cccatggcgc ggatcgtagc ggcgcatctg tgggccagga atcggtacgg cgcccgcagg
     2341 agaaacgggc gccgtaccgg tcagcgcgag cggaggggag gagtacctcg ctcgctggag
     2401 gaagcaagag tcgctggatc agggtttcgg caggtcgatc gtgtgcgtgg agccggagcg
     2461 ggtgcgccgg cggttgggga tggcggagtc gcggtggtgg tcggacgcgg gtgcagttcg
     2521 ggcggccgct cggacttcgg cgacgtgcgc gaaccggcgg cgcaggatcg cctcgatctc
     2581 gggcagggtc cactggaaac catggctcgg gagaccgtgc aggaacgcgg tgagattcgc
     2641 cgattgcccg gcctccaggc cgcgggccag gagggcgcga taggtcgcct tgaggctcgc
     2701 ttcgatccca ggttccatcg actctttgcc gtacagaccg atcggcccgg ccggcgcatg
     2761 ccggtcggtt cggtgaatgg atggtcggtt cggtctggtg cggtggcgat gacccgtcgg
     2821 taccgggtgc ccccggtact tccgtccttc gacctatcct cggcccgtgg acgatctcga
     2881 cgccctcgag cacgagccgg cgaagcgcga tcgcacggcg cggctcatgc gcgtcgctgg
     2941 cctgctcgcc ggcaacccgg atgggatgcg cccgctggac attgccgagc ggctcggcat
     3001 gtcggtccgg aacgtctatc gcgacctgcg ggccctcgag agcgaggtca agctcccggt
     3061 ctggtccgag ggcgggcgct gggggatcct cggcggcgcg ttcctgccgc cgctcaagct
     3121 gtcgctgtcc gaggcgatgg ccgtgttcct cggggcgcgg ctgatggttc ggtacgcgga
     3181 caagtacgac ccggatctcg cctccgcgtt cgagaagctc gccgcgaccc tgccggaggc
     3241 gctgcgcgag cacgtcgcgc gcagcctcga cgtcctgcag gacgcgccgc gcgaccagga
     3301 cttcatcgac cacgtccggc tgctcacccg cgcctgggcc gagcgccgcg tcgtcgagat
     3361 cacgtacgag ggcgccgcct tcgaaggcaa ggtgggcgag cggagccgcc gtgccatcag
     3421 gccatacctc atcgagccgt cactccagac tcacgcgctg tacctgatcg gctgggacga
     3481 ggacaagggc ggcatgcgga cgttcaagat cgagcgcatc cgggaggtcc gcgtgaccca
     3541 gcgaaccttt gacccgcccg aggaggcgac gctcgagcgc gaccttcgcc gcggctggga
     3601 catcatcgcc gaccagcccg cgatcgaggt catcgtgcgc ttctcgacgg ccgtcgcaga
     3661 ccgggtgctg gaaacgacct ggcattccct gcagcgcacc gagcggacgc ccgatggcgg
     3721 gctcgtctgg cgctcgaccg tgagcggcgt gatcgagatc cggctgtgga tcctgtcatg
     3781 gggcgacgac gccgaggtcc tggagccggc ggagctccgg acgcaggtcc gggacatcct
     3841 gggacgggcg gccgcgcagt acgagatgta aaggcgattc gtgcggtggc gtcgattttg
     3901 atgcggtact gcaaggtttg gggtgctgcc cgggggcacc gtgaagtccg cggatgagcg
     3961 gaaatagtac cgatggctgg tcgccattcg agccacgcat cccgaggatc gcctatatgg
     4021 cacgacgtcg cccccgcgcg gcaacgcttg cccgcctggt catggtggcg ctcttcgcgt
     4081 ccctcgcgct gaccaccgcg ggcccgctcg cgaacccttc gatcgtcgcc gcgagcacgg
     4141 ccgacacgat ggaagccgac ctcctcaagt gggtcaacaa cgcgcgcgcc cagcgcggct
     4201 tgaagccgct ccgcctgcat tccgggctcg tggatctcgc cggtgatcag gccgcgaaga
     4261 tggcctcctc cgggagcatg cagcacatca gctgcatgtc ctgcacgttc aacaaccgcg
     4321 gcatccagta ctacagcgtc ggtgagaaca tcgcctacac gacgtacccg tggggcgacc
     4381 tggctgtcgc gagcatctac aacggttgga agggcagctc catgcactgg aacctgctca
     4441 tgagcagcaa gttcaactac gtcggcttcg gcgtcgcgta ccggagctcc agccacaaga
     4501 ccttcgcggc cgccgagctg tccgagtcga aggaccgaac cgacccgtcg cccaggacgg
     4561 gcgccgtgtc gaggagcggc acgaccgtgt cctggtcatg gtccggcgcc gacgtcgcac
     4621 tccagaccca taccgccggc ctcaagaact acgacgtcga gtaccgcgtc ggcagtggct
     4681 cgtggtcgac catcaagagc gcgacgacga cgacatcgct gtcgctgagt ggtcgagcga
     4741 cgggccactg gtacggcctg cggatcaggg cgcgagacaa cgccggcaac gtctcggggt
     4801 actcggcgga gatgaaggtc tgggtgccct gaccggtcgc ggcatcgcgc gcccgttcgg
     4861 cgcgccccta tcctgagcga gtggcgcgcc accccttcgc cgccgtcaac ctcatcagcg
     4921 acccgatcca cggctatctc gagctgacca agcgcctcgc gcgcgacgag tcggctgcgg
     4981 ccggcctgcc cgacgaggac gccgccgagg acgatctcct cgacactgcc tggctgcagc
     5041 gcctgcgccg gatcagccag ctccagagcg cgcgctgggt cttccccacg gccgagcact
     5101 cacggttcac gcatgggctc ggggtcatgc acgaggccgg gctgtgggcg cggacgctgc
     5161 tgccctcgct gcgcacggtc ctgccgacgc tcgctcccac cgagccgctc ccgtccgagg
     5221 ggctcgtcgt cgagacgctg cgggtcgcag gcctgctcca cgacgtcggc cacggcccgt
     5281 tcgcccactt cttcgacgat cgcgtgcttg ccgccttccc cgccccggag gacgcccgcc
     5341 gcggcgccgg caagcggctc tcccacgagg acctcagcca gctgatcatc gagcgcgagc
     5401 tcgggagcgt gattcgggca ctccggcgag cgccgggcgc gaccccggag cgcgacgcgt
     5461 tcgccgacga cgagacgatc gatccccggt gggtctcgtt cctcgtgtcc aagccggccc
     5521 tcatcgacgc gtcgatgccg gcgtgggtgc gactgctcca gccactcctt tcgggcgtgt
     5581 tcacggtcga caacctcgac tatgtccggc gcgacgcgtt cttcacgggt gtgcgggtcg
     5641 gcatcgacgt cgagcggctg cgccggtaca ccttcgtgtc cgagcgcggc ctgaccctgt
     5701 acgaacccgg cgtggcggcg ctcgagggct tcctgggggc gcggatgctg ctccaccagg
     5761 ccgtctactt ccaccgcacc gtgcgcgcga tcgacctcga cctcggcgag gtgttccagc
     5821 ccgcgatcgc cgccctgttc ggcgacgact cgccggccga ccgcctgtcc gactacgtcg
     5881 acctcgacga atacgcgctc ctccaccagg cagctcgctg ggcgcgtggc caggacgtgc
     5941 tcgccgacgg cgcccggggc gagccaggcg gcctgcgcgt cacccgcgcc gtcgccgacg
     6001 gctggcgggg catcctcctg cgccgccctg gctggcgcgc ggaagcggag attcgcgccg
     6061 cctacgaagc gggccttgag ccggaggcag agatcgcgag cctcggacag gcggtccccg
     6121 gcagcgtcgc catcgatctc gccgtcgtcg acgcccggcc tgaaggtcgc acgctggcca
     6181 tcgagggccg cgacggctcg gatatcccag tcaacgaagc cctcgcgcgt gtccccgcgt
     6241 tcgccgtggt cggccggcgg taccgccgcc tctgagcggc atcgcgatcg gcggacgcgc
     6301 gggcgtcggg tgcgtcagtg ccggatgcgc gcgtcccagc cccagcgcat gttggcgctg
     6361 tccatcggcg gaagcacctc gaacgggttg atccgggcga cgatcaggcc cgggagcttt
     6421 gtcagttcga ccgccctcgg attgaggcgc agcgtctccg tctcgttcgg attgaagagc
     6481 gtgtagtgca ggtggcagcc ggtggcgttg ccggtttcgc cctcgtagcc gatcaagtcg
     6541 ccggcgcgga ccacctggcc aatctttacg atcgcgcggg acaggtggga gtacccgctc
     6601 cggtagccat tccgatcgtc gatgacgacg gtaatcgact ggccgcccca cccgacacgg
     6661 tccatcttct ccttgaacgg tgtcaggtcg ccgatccagc ccatgtattt ctggtgcttc
     6721 cggcctgccg cgaggaccac cccatcgtgc gcggcgcgga tgcggtcgcc gcaccacgtc
     6781 gcgatgtcga ggccgtcgtg gaacgatttc ccgcgcagca cgaagcttcc gtccaggcca
     6841 cgtccaaagt cgttcgtgat ggttgcgttt cgcaatggcc atcggtagcc cttgggcggc
     6901 tcgggggcca cgccgcgccg ctcgagccag acgctgcggt cgaagccgag ctcggcgggc
     6961 accgccgcga ggttgccggg cgcgaccgtt ccgcctccga cgtcgtcacc ctggggtccg
     7021 tgcgtcggaa cgttcgccgc ctcgcgcgtt gccgttgccg tccgggtcac gggcgacagc
     7081 cgggcgaggg tgtcatgacc cgggaacagg ccgagccagg cccagctcgt cgcgatcagc
     7141 gcggccgcca gggcaagggc gatcgccccg cgtcgtcgct tgacggcggg gtgggcgtgc
     7201 gcgaaacgcc gaggcgctcg gggatagggg cgagtgtcga tcgcgtcagc ccttggcgcg
     7261 catcttgttg cccttggcgt catgggtcag ctcggcgaga ccgagcgcct ccatgagcgg
     7321 gggcatgtac atcccgaacc ggccgcgata gcccttgcgc aacccgtacc agcctccgac
     7381 cgggttcgac gggtcgcgac cccaggcctc gacggtgccc ggcttcgcgt ccttctgctc
     7441 gtccgccgcg ccgaggtcca tccagtcgcc gtgcgccttg agcgccgcgt gcaggtcgtc
     7501 gatcgcgcgc ttgtcgtact tgagcgtcgt cgagccgacc tggcagacga tcaccggcgg
     7561 atcagcggcg tcatcgatga acatcgtgta ctcggaggta cccggcgggg tcttgaggac
     7621 ccacggatcc gccttcgtgc cactgcccgt cgccactgcc ccgctcctcc cccacgaccg
     7681 tcgattgctg cccgcgaatt tctgcccacg gcagggtagt gcaatgcggg gtcgctgaac
     7741 accctccggc accgaaccgg aacccctcgg actgacctag cctgcccgga tgcgcgtcgt
     7801 ggtggtcggg gctgggtttg ccgggctggc cgcagccctg gagctgcacg atcgtggcgt
     7861 cgaggtggtg gtgctcgagg cgcgcgaccg agtcggtggc cgcgtctggt cgcgggagct
     7921 gccgtcgggc gcggtcatcg agatgggcgc ggagttcatc ctcccgggcg acgcgacgat
     7981 ccgggcggtc acggaccggc tcggactgca gctgttcgcc aagggcacga gctacggcga
     8041 tcgagagccg cgcggcgggc agcccgtgac gcgggaggag ctcgaggccg gctacgcggc
     8101 cgtggcggct gccgcccggg aaggccggct cgggtcgggc tcggtcgtcg gcgccctgcg
     8161 cgagctgccc atgaccgagg gggcgcggga ggcgatccgg gcccgtgtcg aggtctcgac
     8221 cgcatacccg gcggacgatc aggccgcggc cgtgctctcg gagggcggca ccggcaccgg
     8281 cgatttcgcg acgcactcgg tcgccggtgg caaccagcgc atcgcgctgg ccatcgccga
     8341 gcggctgcgt ggggccgtgc acttgagggc cgaggtggtg tacgtcgcgt gggcgctcgc
     8401 cgggtcggtc gatgggccgg ccgcggcgcc ggccggggtt cgcgtccggg caggcggcga
     8461 cgtcatcgaa gccgaagccg cggtgatcgc ggtgccggcg agcctgatcg atcggatcgc
     8521 cttcgatccc ccgctgccca cctggaaagc cgccgcactg gcggccgtgc ggtacgggca
     8581 cgcggcgaag ctgttcctcc cgctcgacga gctcgcgccg cccagcgcga cgctctctgt
     8641 cccggaccgg ttctggacgt tcacccagca cgcgcaggac gggtcgccgt tgcccgtcgc
     8701 ggcctcgttc gtcgg
//
LOCUS       DATBSW010000250         4695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_729351, whole genome shotgun sequence.
ACCESSION   DATBSW010000250 DATBSW010000000
VERSION     DATBSW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729351"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VIF63_06935"
     CDS             <1..537
                     /locus_tag="VIF63_06935"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239153.1"
                     /translation="CGSGGSACSMHVAHGATVKLTAATEPGSTFKRWEGACSGSSTTC
                     SVKMADDRSVEAVFAKVATPPPATPKPTAAPKPTATAKAPTATQHVATAAPTTIPSEA
                     AATGTPAPSAIGATTSPGAPTGTPAGALLSPAASIAPSVVAATDSGVGVIALAIVLAG
                     LLIGVGVGVNAYMRRKRT"
     gene            complement(617..1003)
                     /gene="trxA"
                     /locus_tag="VIF63_06940"
     CDS             complement(617..1003)
                     /gene="trxA"
                     /locus_tag="VIF63_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8239154.1"
                     /translation="MAIKTATDATFEAEVLQNDKPVVVDFWAAWCGPCKLVAPEMEKL
                     AAKYEGAIDIVKVDVDANPGLSRAFNIMSIPTIAFFAPGKQPMGVVGFRPAEQLEVQF
                     SLASYPKIAEAAAATTDAPAATSDAA"
     gene            complement(1072..1389)
                     /locus_tag="VIF63_06945"
     CDS             complement(1072..1389)
                     /locus_tag="VIF63_06945"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8239155.1"
                     /translation="MAIEIAASQAAADERETGYQRTAVVGRALADPKRLCVLESLAAG
                     ELSVSELAGRVACQVPNMSQHLAVLRSAGLVSTRREGSTVFYRLSDPRVLEAFSLLQS
                     IAR"
     gene            1465..2490
                     /locus_tag="VIF63_06950"
     CDS             1465..2490
                     /locus_tag="VIF63_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8239156.1"
                     /translation="MAAETDLDPASVDLRAIEAAREVIAPHVHRTPLLSSTAAASVVH
                     RATGHRLADGRVYLKAEHLQKTGSFKPRGMTYRVATLGPEERRRGIITVSAGNAAQGY
                     AYAGAVFGVPVTVVMPAAANPMKAAAAAGYGARVVLEGAHVGETFAAKDRIAAEEGLT
                     FCHPFDDANVVAGHGSVGLELLEDLPEVDVVIVGIGGGGLISGVATALKAKRPGVRVI
                     GIEPTGSNALGQALANGAPVTIAPKSVADGLNAPFAGALTLELVQRFVDDVITIDDSI
                     ILAGLKFAIERTKQALEPAGAAALGALLAGSVAVRDGERVAVVLSGGNVDTSRLGEFL
                     AAAAPLA"
     gene            complement(2530..2943)
                     /locus_tag="VIF63_06955"
     CDS             complement(2530..2943)
                     /locus_tag="VIF63_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY8239157.1"
                     /translation="MAKRPRGSHRGIARAAARAAAFDRLVDRAVGAIPEPYREALREV
                     AIVVEDEPSRDQLRENGLGPDESLYGLYEGVPRVEYAADWVTSPNRITLFRLPLEEDF
                     ADPLELEAEVRLTVLHELAHHMGIDDDRLDELGAS"
     gene            3024..4097
                     /locus_tag="VIF63_06960"
     CDS             3024..4097
                     /locus_tag="VIF63_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY8239158.1"
                     /translation="MKVVHTERHRAHDPQVETYLGVPVPANEVPARADAILAALAADG
                     SFEVVEPTAHGFDPITAVHDPGLVRFLKEAWPESQRQRIERDFLVADTYATRAMFQGM
                     SEAFIAARPEPEAVGGRAGWWGLDSSNPIVGGSYDAALGAVDVALTAADIVLGGEAVA
                     YGLCRPPGHHAAAAMAGGYCFFNNAAIAAHAIAAATGERVAILDVDVHHGNGTQQIFW
                     RRADVLYASLHADPKRLYPFFLGHVDETGEGPGAGANLNLPQPLRADDADYLKDFERA
                     LEWIDASAGSIVVVSLGFDTYHLDPIGDLALTTEGYHAMGRRVAGLGRRLVILQEGGY
                     YLPDLGANAVAWLRGASGRERGG"
     gene            4148..>4695
                     /gene="folE"
                     /locus_tag="VIF63_06965"
     CDS             4148..>4695
                     /gene="folE"
                     /locus_tag="VIF63_06965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEY8239159.1"
                     /translation="MTATDLPEHVRKLVAEIETEMGEEHGLNGREADEERTRPIVGTG
                     SLHTPKAPVEEAVRGILLEIGEDPDRQGLVGTPDRVHRMYAELTAGYHVDEDRLINGA
                     VFDVEYSEMVVVKDIPFYSLCEHHLLPFFGAAAVAYIPRGKVIGLSKIPRVVEMYARR
                     LQVQERLTQQIADFLQDRLGPQG"
BASE COUNT          692 a         1656 c         1670 g          677 t
ORIGIN      
        1 tgcgggtctg gcgggtctgc ctgcagcatg cacgttgccc acggcgcgac cgtcaagctg
       61 acggccgcca ccgagcccgg gtcgaccttc aagcgctggg agggcgcatg cagcgggtcg
      121 tcgacgacct gctcggtcaa gatggccgac gaccgctcag tcgaggcggt cttcgcgaag
      181 gtggcgacgc caccgccggc cacgccgaaa cccacggctg cacccaagcc cacggccacg
      241 gccaaggcgc caacagcgac gcagcatgtc gcaacagctg caccgacgac gatccccagc
      301 gaagccgccg caaccggaac ccccgcgccg agtgccatcg gcgccacgac ctcgcccggg
      361 gcaccgaccg ggacgcccgc cggtgcgttg ctatcgcctg ccgcgagcat cgcgccgagc
      421 gtcgtcgcgg cgacggactc gggcgtcggc gtgattgccc ttgcgatcgt cctcgctggg
      481 ttgctcatcg gcgttggcgt tggggtcaac gcgtacatgc ggcgcaaacg gacataggcg
      541 ccgcgatgcc ggacccatcg accctggaat cgaagagccc gccccggagg gcgggctcga
      601 tcgtttgtgc ggggagctac gcggcgtcgc tcgtcgcggc cggcgcgtcg gtagtggcgg
      661 cagccgcctc ggcgatcttc gggtaggagg ccaggctgaa ctgcacctcg agctgctcgg
      721 cgggtcggaa gccgaccacg cccatgggct gcttgcccgg cgcgaagaat gcgatcgtcg
      781 ggatcgacat gatgttgaac gcgcgggaca ggcccgggtt cgcgtcgacg tcgaccttga
      841 cgatatcgat cgcgccttcg tacttggccg cgagcttctc catctcgggg gcgacgagct
      901 tgcacggccc gcaccaggcg gcccagaagt cgaccacgac cggcttgtcg ttctggagga
      961 cttcggcctc gaaggttgcg tcggtggccg tcttgatcgc catcgctgat tgatcctttc
     1021 tcccgctgga ggcgggctgc ccggcgaacc gggcgacgtt gcgttcgacc cttagcgggc
     1081 gatcgactga agcaggctga aggcctcgag gacccgcggg tccgagaggc ggtagaacac
     1141 cgtgctgccc tcgcgccgcg tcgacacgag gccggcggag cggaggacgg ccaggtgctg
     1201 gctcatgttg ggcacctggc aggcgacgcg gccggccagc tcgctgaccg agagctcgcc
     1261 cgcggcgagg gactcgagca cgcaaaggcg ctttgggtcg gcgagcgccc gaccgaccac
     1321 ggccgttcgc tggtagcctg tctcacgctc gtcggcggcc gcctgggagg cggcaatctc
     1381 gattgccatc gctcgtacga tctcacaact tgcgcaatcg cgcaactcgc ggggcaaatc
     1441 gggacgcgct tgtaccatcg cccgatggcc gctgaaaccg atctcgaccc ggcgtcggtc
     1501 gacctgcgcg cgatcgaggc ggcgcgcgag gtcatcgcgc cccacgtgca tcggacgccc
     1561 ttgctgtcct cgaccgccgc cgccagcgtc gtccaccggg cgaccggcca ccgcctggcc
     1621 gacggccgcg tgtacctcaa ggccgagcac ctccagaaga cgggttcgtt caagccgcgc
     1681 gggatgacct accgggttgc caccctcgga ccagaggagc ggcgccgcgg gatcatcacc
     1741 gtctccgccg gcaatgcggc ccagggctac gcctacgccg gtgcggtgtt cggcgtgccc
     1801 gtgaccgtcg tcatgccggc cgctgcgaac ccgatgaagg ccgcggccgc ggccggctac
     1861 ggcgcccggg tcgtgctcga gggagcgcac gtcggcgaga cgttcgccgc caaggaccga
     1921 atcgcggcgg aggaagggct gacgttctgc cacccgttcg acgacgcgaa cgtggtcgcg
     1981 ggtcacggct cggtcgggct ggagctgctc gaggacctgc ccgaggtcga cgtcgtcatc
     2041 gtcgggatcg gcggcggcgg gctgatctcg ggtgtcgcga cggcgctgaa ggccaagcga
     2101 ccgggggtcc gcgtgatcgg catcgagccg actggttcga acgccctggg ccaggcgctc
     2161 gcgaacgggg cgccggtcac gatcgcgccg aagagcgtcg ccgacgggct gaacgcgccg
     2221 tttgcgggcg cgctgaccct tgagctcgtg cagcggttcg tcgacgacgt gatcaccatc
     2281 gatgactcga tcatcctcgc cggcctgaag ttcgccatcg agcggacgaa gcaggcgctc
     2341 gagccggccg gcgcggctgc cctcggcgcg ctgctcgcag ggtccgtcgc cgtccgcgac
     2401 ggcgaacggg tggcggtcgt cctgtcgggc gggaacgtgg atacgagccg gctcggggag
     2461 ttcctcgcgg cggcggcgcc gctggcctga ccgagcgcgc ctcttcgggc tccggcctgt
     2521 cgggggcgcc tagctcgcgc ccagctcgtc gagacggtcg tcgtcgatgc ccatgtggtg
     2581 ggcgagctcg tgcagcaccg tgagccgcac ttcggcctcg agctcgagcg gatcggcgaa
     2641 gtcctcctcg agcgggagtc ggaacagcgt gatccgattg ggcgacgtca cccagtcggc
     2701 cgcgtactcg acgcggggca cgccctcgta caggccgtac agcgactcgt ccgggccgag
     2761 gccgttctcg cgcagctggt cgcgcgacgg ctcgtcctcg acgacgatcg cgacctcgcg
     2821 cagcgcctcg cgatacggct ccgggatggc gcccactgcg cgatcgacga ggcggtcgaa
     2881 ggcggccgcg cgggcagcgg cgcgagcgat gccccgatgc gagccgcgcg gacgtttggc
     2941 catcgggcgg atcgtagccc gcgctcgcgg cgggacgccg cacgggagac cgcgccggca
     3001 acgacgccgc tacgatccgc ggcgtgaagg tcgtccatac cgagcgccat cgcgcccacg
     3061 atccgcaggt cgagacctac ctcggcgtgc ccgtgcccgc caacgaggtg cccgcccgcg
     3121 ccgatgccat tctcgccgcg ctcgcggctg acggtagctt cgaggtcgtc gagccgaccg
     3181 cgcacgggtt cgacccgatc acggccgtgc acgatcccgg actggtccgc ttcctgaaag
     3241 aggcctggcc cgagagccag cgccagcgca tcgagcggga cttcctcgtc gccgatacct
     3301 atgccacgcg agcgatgttc cagggcatga gcgaggcgtt catcgccgcg cggcccgagc
     3361 ccgaggcggt cggcgggcgt gcgggctggt ggggcctcga ctcgtcgaac ccgatcgtcg
     3421 gcggcagcta cgacgcggcc ctcggcgccg tcgatgtcgc gctcaccgct gcggacatcg
     3481 tgctcggggg cgaggcggtg gcgtatggcc tgtgtcgccc gcccgggcac cacgcagcgg
     3541 cggcgatggc cggcggctac tgcttcttca acaacgccgc catcgcagcg cacgccatcg
     3601 cagcggcaac gggcgagcgc gttgcgatcc tcgacgtcga cgtccatcac ggcaacggca
     3661 cgcagcagat cttctggcgc cgcgccgacg tgctgtacgc ctcgctccac gccgacccga
     3721 agcgcctgta cccgttcttc ctcggccacg tggacgaaac tggcgagggc ccgggcgcgg
     3781 gcgcgaacct gaacctgccc cagccgctcc gcgcagacga tgccgactac ctcaaggatt
     3841 tcgagcgagc gctcgagtgg atcgatgcct cggccggttc gatcgtcgtc gtctcgctcg
     3901 gcttcgacac gtaccacctc gacccgatcg gtgacctcgc cctcacgacc gagggctacc
     3961 acgcgatggg acgtcgcgtc gcaggcctcg ggcgccgcct cgtgatcctc caggagggtg
     4021 ggtattacct gcccgacctc ggcgcaaatg ccgttgcctg gctgcgcggc gcgtcgggcc
     4081 gcgaacgggg cggctgaccg ctcgatcggc gggcgactcg cgcatgggga gtacgatccc
     4141 ccgagccatg accgccaccg acctgcctga gcacgttcga aagctcgtcg ccgagatcga
     4201 gaccgagatg ggcgaggagc acggcctgaa cggccgagag gccgacgagg agcgcacgcg
     4261 cccgatcgtc ggcaccggat ccctgcacac gccgaaggcc ccggtcgaag aggccgtgcg
     4321 cggaatcctg ctcgagatcg gcgaggaccc ggatcgacaa gggctggtgg gaacgccgga
     4381 tcgcgtccac cggatgtacg ccgagctgac ggccggctac cacgtcgatg aggatcggct
     4441 gatcaacggc gccgtcttcg atgtcgagta ttcggagatg gtcgtcgtca aggacatccc
     4501 gttctactcg ctgtgcgagc accacctgct gccgttcttc ggcgccgccg cggtcgccta
     4561 catcccgcgg ggcaaggtca tcgggctgtc gaagatccca cgcgtcgtgg agatgtacgc
     4621 ccgacggctc caggtgcagg agcggctgac gcagcagatc gccgatttcc tgcaggaccg
     4681 ccttggcccg caggg
//
LOCUS       DATBSW010000251        15673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_164712, whole genome shotgun sequence.
ACCESSION   DATBSW010000251 DATBSW010000000
VERSION     DATBSW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164712"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VIF63_06970"
     CDS             <1..1086
                     /locus_tag="VIF63_06970"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY8239160.1"
                     /translation="LFGALLLATLVACSPAVVAPAAAPTAAPPQPTIAITPSLEPPPS
                     LAPFPTPTLEPGAFVPADLDGVLTSPELAHRLPIGVSIDDSRAARPQSGFNAASMVWQ
                     AHADGYESRYLLVFQEQDSAAIGPVRSARNFLAHWTAELRGALAHYGGDRLTRQWMVA
                     HRRELFTDIDGMGAGNPAYHRTHDRAAPHNAYTSTADLWRVAGKLGGAAAINAAVHVR
                     PFRDDSPETLRGASQAITVPYHTVSIGYRYDPATYAYLRSVNGKPHIDPGDGKRVTAR
                     TVVVLFMKFRTDSHIEPGHNRPVLTYIGSGKALFFMEGKVVQGSWSKTGEVRPTMFLG
                     PDGNELPLIRGRIFVQVVMPSTKVTVH"
     gene            1164..2465
                     /locus_tag="VIF63_06975"
     CDS             1164..2465
                     /locus_tag="VIF63_06975"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HEY8239161.1"
                     /translation="MATALYLNVAQYDRYVADDYCSAASLATNGFLGAQTDYFRGWTG
                     RFATTALVNAVEVFGPGALPSFVGLLIAGLAMAIAAAARAVFAAGVGAAAAGVVTTFL
                     IVAAAPQPFQTFYWLSGAANYVPPLILGATAVALAAQARRDPRARWLAALALVGFVGA
                     GFSEAATAFALAVGVLLLGFAAIGRPELRTALPAVSIFVIASIGGAVIVALAPGNLAR
                     FAQLPPPDLSRAIALTPGESAGRVAELVAANIGLEVFVVLLVATLAWARRLPSLPPRG
                     FLAATLAALVLVPVTIFPALWATSTEPRARTLVYLAALLLAWFATLGWATGSVLGRRS
                     PLRPGALGAAALVLAIVPLATFVDVQAARSELGRWAAGQDQLDKLLANSAHGTVEATW
                     KPIPAHGLLAVDLRPPGSDPKLWVNDCLARFHHLDALIVRP"
     gene            2519..3934
                     /locus_tag="VIF63_06980"
     CDS             2519..3934
                     /locus_tag="VIF63_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8239162.1"
                     /translation="MTGTPTERQRPRPAATIAAILSFVWPGIGQWYGGRLRVALVFAI
                     PILVAAIALVIWIGGGLQSAAIEMIDPAVASGVLILIVLLAAWRIIALLEAAYAIDRR
                     RAFRGRAGVALGVLVALVLASHGLVASYAWAFYEAGSQIFVPEPTAQVPQSSESPGPT
                     ELYVATPFATPASDTARITILLTGIDKNAERTHSLTDTLLVLSLDPETDTAAMISLPR
                     DVSEFPLYNGGLYRGKINSLMSYADRHPKDFPDGPLPTLAHELGYLLGIPIHYYAAVD
                     LGGFEQLIDAAGGVDIDVKRAILDGRYDWLDGSDTGFFLSVGPHHLNGRIALAFVRSR
                     QGVGDNDFVRADRQQQLLLALRKRLLDPAMVTKLPELLDAAAKTIRTNFPPDRINEML
                     ALAEQFDTARIRRYVLRPPTYSVHPPTNTTNGTYILRLRIDAIARLSVKLFGADSAYW
                     TGAFDPAGSPIPGPLPSPVPN"
     gene            complement(3942..5330)
                     /locus_tag="VIF63_06985"
     CDS             complement(3942..5330)
                     /locus_tag="VIF63_06985"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY8239163.1"
                     /translation="MAFRLSLMVADGMSAAGLFVMLSLTRFGADNWTSSWRKAGIDPV
                     WVLAAYALAWVVALWLNGLYRNRVRWSARSEAIDVLRTGIGLAILTFSALFILKMPDV
                     SRLFLLVLFPAQIAVTIAIRFALRIAFRVARSNGFSRRFVLIVGTDGNAEAFARRIER
                     HRELGLRVIGHLHWTKSKRAPTVTRPILGRVDDIVQVLHASVVDEVAICLPPAAWGMI
                     EPIARLCEEEGKIVRVPLFDGMLSLPGAHQEEFDGIPILSLVYGPDRIIALVGKRVLD
                     ILGSATALVLLSPVLLAVALWILRRDGRPVLFRQPRVGLHGRAFEVVKFRSMVPDAEA
                     RLPDLLEQNEIQGHAFKMQDDPRLSSTGGWLRRTSIDELPQLWNVLKGEMSLVGPRPP
                     LPREVSGYDVWHRRRLSMKPGITGLWQVSARNEEEFDRWVEMDLDYIDRWSLWLDLKI
                     MLRTIPAMLQGR"
     gene            complement(5460..6821)
                     /locus_tag="VIF63_06990"
     CDS             complement(5460..6821)
                     /locus_tag="VIF63_06990"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY8239164.1"
                     /translation="MNSPRRRLAVIGAGYVGLVTGACLAGLGHTVRFVETNPERLEAL
                     RRRHIPIHEDGLQAAFDAGLDNGTIEVVARATTDVDAALVCVGTPIDADGQSDMSQVR
                     SALADLGPLVESGVPLVMRSTMPPGATRALAEWSGARTARLVTNPEFLRQGTALHDFL
                     HPSRIVVGTFPDVDQGVLDLVLGLFDGLPGERMVVSVAAAELIKNGSNAFLALKLSYA
                     NEVASLSEEYGADVDEVLAGIAADPRIGSQYMRPGLGFGGSCLPKELKVLEAAGLARG
                     LAMHVTAAASNANQATQARFAARIERALNGLPGRRIALLGLAFKAGTDDVRESPALGT
                     ATRLIEGGAEVVGYDPVASMNAQRALPGLEVAASVDAAVRGADAIVIATEWPEFAEID
                     WPGLRSIVRQPLIIDGRRLLEPEPIRRAGYRYLAIGRLGEDERTGRPREAVPAGARAA
                     GAR"
     gene            complement(6844..8058)
                     /locus_tag="VIF63_06995"
     CDS             complement(6844..8058)
                     /locus_tag="VIF63_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8239165.1"
                     /translation="MQAARPRPIVLMLVYERYERDSRVRRHVRALVAGGYDVRIVGVG
                     GREAEAAALADGGRLRSLGAAKYRGGSRLAYVLAYAGFTFRALIEVARMPGSQLAVVW
                     VNAPPDVLVFAALPARLRRIPVVLDVHDITSQLFEAKFGAASRLVARLIRLVETAAYR
                     FASAIVTVHAPYRDQIQQRVPGKPVIDVLNVPDEQAWYEEGRARAARPIGRARTALRV
                     GHHGTIAERFGVDLAVRGVAALHAAGTPVRLSILGDGDYAPAIEELIDSLDARDVISF
                     DRRTFLPDEIPAFVAGVDVGVAPYHESQFVDSILPVKVLEYLALGVPAIATSTSVLRA
                     YLPADTVTYVSPASAETVRDAIASLVDADRRAAFARAGVEALAGLSWTEQRLRLLMTI
                     DALIAGRKPGKP"
     gene            8145..9620
                     /locus_tag="VIF63_07000"
     CDS             8145..9620
                     /locus_tag="VIF63_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY8239166.1"
                     /translation="MPGPAAPQDEHAQARGSGLALDSAMSMAALVMLGGTRVVYGSIL
                     SHATDRETFGVVGLLIGLTMIGSFLLPAGIGSAISRYIPFNLGRGDAAAARGLYRLLL
                     RVALVAGLPLAIGAFAIARLALDRPTGQSIEVALLMLVYALYTVCKAGLYGFGRVGFY
                     LRLEIISSVLVLALALAVAVTGSTSYLAPLIVGYGAFAAAAAYRLRTDAAGAASAPDP
                     GLKREVGGFVILACLGTLASAGFLQSTQFLAATFAPVAQVASFAAAVTLVAPVQFLPR
                     ALALAMFPWMAAAHGAGDVDAIRRQVDLSTRALVVVLTPPFAAGIILAPEILKVFGGE
                     AYVDGAHVLQLILVGAFLSVIQVGSVNALSSASDWQLRIPVAWAVIGALSGLAFVALA
                     GPVFGSTGVAGGYILGTLVSAAGPIAVVWRRWSMPWGGVFARATIAIAMSLIVAVLVP
                     SRAAAASEVILDVVLAGIAASIAAAVLARDIRAIVVARRRP"
     gene            9622..11118
                     /locus_tag="VIF63_07005"
     CDS             9622..11118
                     /locus_tag="VIF63_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY8239167.1"
                     /translation="MASPAITEPRRTLGATSIGRPGGSSLVRGGAWLLLVAVLAAVTL
                     EGLVASSLGPAAGDLLRIGRNAAYLVLAPFLLGYVVLTGRIRAFLQPVDIALAVLVAV
                     MFIGSLAADSSIRLTGEGMFVYLRGTIIFYAIRALRPSWADARRLAWLLGGIFVVNAI
                     VAVIQGFVGLPAFTFFGFTDLTWAVINRAQGLQVHPNHLGHILGLGSMVVLGLIASKP
                     RVPARWWALFALFAYAIAASQSRETVLGMVAGLIVLLILRRSAIKRLVAAGTVLVAFV
                     AVIWIAHPANFAELTRRLTGVVTAIEIPSGQEEGVVCDPSVEPCTIEGVPHREVRVLY
                     IQQGLALWTRQPVFGYGIGQFGGAVAFEDDPRWYLDPRFGPNGFSMHGFEAKQVDSFW
                     LHLFVEVGAAGSIAYAIWYLLLGWPLARWSWPRRRSALADGATDLALSLQPAAVAALA
                     FAWVVAVFSPSLEDPMFPPLLFALLGFAWLTRPADTSERPMDPAPGPA"
     gene            11121..12125
                     /locus_tag="VIF63_07010"
     CDS             11121..12125
                     /locus_tag="VIF63_07010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8239168.1"
                     /translation="MSEAAGLTRPLRVLFVTNMWPEHGGFRGTFVEQQASSLRELGHH
                     VDVEIVAQARGIADYVLAAPRVRRRAREGRYDVVHVHFGLTAFAARLVDRPRVITLYG
                     SDINERLKRWWTRLSWGGAAARVYVSRSLAAVAGDRAGRVVPNGVDFDLFRPGERTAA
                     RTELGVGPDDRLILFGSQPERAVKGHDVFADVVRRLRSQGIPARELILTSPGQAASRV
                     VAKYDAADVLLFTSRRGSEGSPTVVKEAIAMGLPVVSVDVGDVAEMLAGIEPSEVVAF
                     PKEGSVGAARDMLVGRLADATARVLANGARADGRERLSWLSLDQVAGRVVDVYRSVAT
                     "
     gene            12122..13051
                     /locus_tag="VIF63_07015"
     CDS             12122..13051
                     /locus_tag="VIF63_07015"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8239169.1"
                     /translation="MTVALVTGGLGFVGAHLCGLLRDRGWEVVVVTRRTVGGPPGVRL
                     VSLDVTDPQLVDDAIAEIRPDVTFHLAAAMAQRGTPAEAILSNAVVGTLNVCSSLRRR
                     GRPGSRLILAGSSAQYGDVPRAENPVAEDAPQRPVNAYGFAKVAAEAVACALAADGSL
                     DVIPVRAFNHVGPGESGTTVAGAFALRISDVLRGAASRVDAGGLDAVRDFTDVRDIAA
                     GYLAAAERGRPGRPYNLCSGRATSIGTILDGLLREAGVDRSIVDEVPTERGGIPFQVG
                     SPIRARDELGWEARIPLDQSLADLLAVTALRSA"
     gene            13048..14061
                     /locus_tag="VIF63_07020"
     CDS             13048..14061
                     /locus_tag="VIF63_07020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY8239170.1"
                     /translation="MSGRDDTYWNGRRVSVSGAGGFIGSHLAERLVRAGAQVRAMVRY
                     NGRSELGSLAHADADVRAAMEIIHSDVTDPYAVRRFVEGSDTVFHLAALIAIPYSYAA
                     PASYVATNVSGTLHMLEAVRDLGIAHMVQTSTSETYGTARTTPIDEDHPLQPQSPYAA
                     SKVGADALAESFHRAFDVPVATIRPFNTFGPRQSARAVVPTIASQVLAGRSEIRLGDL
                     RPRRDLTYVADTVEGFLAVARAPACVGMVTNIGSGTAVSIGELAALIAELAGKPGLPI
                     VTDAVRVRPEGSEVFELVADAHRAAELTGWRPEVSLRDGLRQAIEFVRNHPDAYDPDR
                     YAT"
     gene            14058..14771
                     /locus_tag="VIF63_07025"
     CDS             14058..14771
                     /locus_tag="VIF63_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8239171.1"
                     /translation="MKAAVLAGGKGTRLRPFTTTFPKPLMPMGEMPILELLLRQLRAH
                     GVDEVTLLTGHLAYLIEGYIGQGESLGLSVDYVHEAEPLGTAGPLRSLVGRMTDDFLV
                     MNGDLLTDVDFTALMDHHRASRATVTISTFNREERLELGVLQSAPDGAITGYDEKPQL
                     NLTVSMGLYAMSPAALRLIPDGYYDMPDLIRDLLAAGQPVIGRRHEGTWYDIGRPDDY
                     AAANELFARDPRAFLAPER"
     gene            14779..>15673
                     /gene="wecB"
                     /locus_tag="VIF63_07030"
     CDS             14779..>15673
                     /gene="wecB"
                     /locus_tag="VIF63_07030"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY8239172.1"
                     /translation="MSGSAASRVAVLFGTRPEAIKLAPVILALRSAGLEVTVVATGQH
                     RELASEVLPQFALDIDVDLALMRPGQSLDYVLGEGIQAVGRYLVEAKPDAVIVQGDTS
                     STLAAALAAFHHRVPLAHVEAGLRTHDLGFPFPEEMNRRVTSVVARWHFAPTQRAAEH
                     LAAEGIVDRVYVTGNTVVDAARHVLAAAALPAELADLGNGARYIVATAHRRESWDGGI
                     ERIARALRTTLAAVDDIQLVFVTHPNPLAAGPVTAILQGEARARVVTAIPYPSFLALL
                     RGAVVAVSDSGGVQEEGATLGV"
BASE COUNT         2128 a         5676 c         5405 g         2464 t
ORIGIN      
        1 ctgttcggcg cgctgctcct ggccacgctc gtcgcctgca gcccggcggt ggtcgcgccg
       61 gccgccgcac cgaccgcggc gccgccccag ccgacgattg cgatcactcc atcgctcgag
      121 ccgccgccgt cgctggcgcc gttccccacg ccgacgctcg agccgggcgc gttcgtgcca
      181 gcagacctcg acggcgtgct cacgagcccg gagctcgccc accgcctgcc catcggcgtg
      241 tccatcgacg acagccgggc agcacgaccg cagtcgggct tcaacgcggc gtcgatggtc
      301 tggcaggcgc acgcggacgg ctacgagtcg cgctacctgc tcgtgttcca ggagcaggat
      361 tcggccgcga tcgggccggt ccggagcgcg cgcaacttcc ttgcccattg gaccgccgag
      421 ctgcgcgggg cgctcgccca ttacggcggc gatcggctga ctcggcagtg gatggtcgcc
      481 cacagacgag agctcttcac cgacatcgat ggcatgggcg ccgggaatcc cgcgtaccac
      541 cgcacccacg atcgagccgc gccgcacaac gcgtacacgt caaccgctga cctgtggcgg
      601 gtcgccggca agctgggtgg ggctgccgca atcaatgcgg cggtccacgt ccggccgttc
      661 cgcgatgact cgcccgagac ccttcgcggc gcgtcgcagg cgatcaccgt gccctaccac
      721 acggtctcca tcggctaccg ctacgacccg gccacctacg cctacctgcg gagcgtgaat
      781 ggcaagccgc acatcgatcc aggcgacggc aagcgggtca ccgcgcgcac cgtggtggtc
      841 ctgttcatga agttccgcac cgacagccac atcgagcccg gtcacaatcg gccggtgctg
      901 acgtacatcg ggagcggcaa ggcgctcttc ttcatggagg gcaaggtcgt ccagggaagc
      961 tggtccaaga ccggtgaggt caggcccacg atgttcctcg gccccgatgg caacgagctg
     1021 ccgctgatcc gcggcaggat cttcgtccag gtcgtcatgc cgagcacgaa ggtgaccgtc
     1081 cactgagcca cccggcgcag ggctcggcct actgggcgag catcctggcc cacgggcgat
     1141 gggtaccggc gctggccgcc ggtctggcga ctgcgctcta cctcaacgtc gcgcagtacg
     1201 atcgatacgt cgcggacgac tattgttccg cggcctcgct cgcgacgaat ggcttcctcg
     1261 gcgcccagac ggactacttc cgaggctgga ccggccgctt tgccacgacc gccctcgtga
     1321 atgccgtcga ggtgttcggg ccgggtgccc ttccgtcgtt cgtcggcttg ctgatcgccg
     1381 gattggcgat ggcgattgcc gcagccgccc gggcagtgtt cgccgcaggg gtcggcgcgg
     1441 ccgccgccgg cgttgttacc acattcctga tcgtcgcggc cgcgccacag ccgttccaga
     1501 ccttctactg gctctccggc gcggccaact atgtgccgcc actgatcctt ggggccaccg
     1561 ccgtggccct ggcggcgcaa gcacgccgtg acccgcgcgc tcgatggctt gcggcactcg
     1621 ctctggtcgg tttcgttggg gcgggattct cggaggccgc cacggccttc gccctggcgg
     1681 tcggcgtcct cctgctcggc ttcgctgcca tcggtcggcc cgaattgcga accgccctgc
     1741 cggcggtttc gatcttcgtg atcgcgtcga tcggcggcgc ggtgatcgtc gcgctcgctc
     1801 cgggcaacct cgcccgcttc gcacagctcc caccgcctga tctttcccgg gcgatcgcgc
     1861 tgacaccggg cgaatcggcc ggacgggtcg cggagctcgt agccgcgaac atcggtctcg
     1921 aggtcttcgt cgtcctgctc gtcgccaccc tcgcgtgggc ccggcgcctg cccagccttc
     1981 ctccacgagg attcctcgcc gcgacgctcg ctgctctcgt cctggtacca gtcacgatct
     2041 ttcccgctct ctgggcgacg tccacggagc cgcgcgcccg tacgctcgtc tacctcgcag
     2101 ccctcctgct tgcctggttc gcgaccctgg gctgggcgac cggcagcgtc ctcggccgtc
     2161 ggagcccgct ccggcccggc gcacttggcg cggccgcgct cgtgctcgcg atcgtgcctc
     2221 tcgcgacgtt cgtcgatgtt caggccgcgc ggtccgagct cgggcgatgg gcggccgggc
     2281 aggaccagct cgacaagctg ctcgcgaatt cggcgcacgg gaccgtcgag gccacgtgga
     2341 agccgatccc ggcgcacgga ctgctcgccg tcgatctccg gccgcccggc tctgatccga
     2401 aactctgggt caacgactgc ctcgccaggt tccatcatct cgacgcgctg atcgttcgcc
     2461 cgtagccctt cggcgcgccg tcgggagtgc ctgacgggtc gtgctaggct ccgccgcctt
     2521 gactggaacc ccgaccgagc gacagcggcc gcgtcccgcc gcgaccatcg ccgcgatcct
     2581 ctcgttcgtc tggcccggca tcggccagtg gtacggcggc cggttgcggg ttgccctcgt
     2641 gttcgcgatc ccgatcctcg tcgcggcgat cgccctggtc atctggatcg gcggtggcct
     2701 gcaatccgcg gcgatcgaga tgatcgaccc cgcggtcgca tcgggcgtgc tcatcctgat
     2761 cgtgctgctc gcggcctggc gcatcatcgc gttgctcgag gccgcctatg cgatcgaccg
     2821 gcggagggcg ttccgagggc gcgccggcgt cgcgctgggc gtcctcgtcg ccctcgtcct
     2881 ggccagccat ggcctcgtcg cgtcgtatgc ctgggccttc tacgaggccg gcagccagat
     2941 cttcgtcccc gagccaaccg cccaggtgcc gcagtcgtcc gagagccccg gcccgactga
     3001 gctctacgtc gccacgccgt tcgcgacccc ggcgtcggac accgcgcgga tcaccatcct
     3061 gctgaccggg atcgacaaga acgccgagcg gacccactcg ctgacggaca cgctcctggt
     3121 cctgagcctg gaccccgaga cggacacggc ggccatgatc agcctgccgc gcgacgtctc
     3181 ggagttcccc ctgtacaacg ggggcctgta ccggggcaag atcaactcgc tcatgtccta
     3241 cgccgaccgg caccccaagg attttcccga cggaccgctg ccgacgctcg cgcacgagct
     3301 cgggtacctg ctcggcatcc cgatccacta ttacgcggcg gtcgacctcg gcgggttcga
     3361 gcagctcatc gacgctgccg gcggcgtcga tatcgacgtg aagcgggcga tcctcgacgg
     3421 ccggtacgac tggctcgacg ggtcggacac cgggttcttc ctgtcggttg gaccgcacca
     3481 cctcaacggt cggatcgcgc tggcgttcgt ccggtcgcgc cagggcgtcg gcgacaacga
     3541 tttcgtgcgc gcggatcgcc agcagcagct gctcctggcg ctccggaagc ggctcctcga
     3601 ccccgcgatg gtgacgaagc tgcccgagct gctcgatgcc gcggcgaaga ccatccggac
     3661 gaacttcccg cccgaccgga tcaacgagat gctcgcgctg gcggagcagt tcgacacagc
     3721 ccgcatccgc cgctacgtgc tccggccgcc gacgtattcg gtccacccgc cgaccaacac
     3781 caccaacggg acctacatcc tccgcctgcg catcgacgcg atcgcccggc tctcggtcaa
     3841 gctgttcggt gcggacagcg cgtactggac cggcgccttc gatcccgcag ggtcgcccat
     3901 ccccggaccc ctcccgtcgc cggtgccgaa ctgaccggcg ctcagcgccc ctggagcatg
     3961 gccgggatcg tgcggagcat gatcttgagg tcgagccaga gcgaccatcg atcgatgtag
     4021 tcgaggtcca tctcgaccca gcggtcgaac tcctcctcgt tccgggcgct cacctgccag
     4081 aggccggtga tgccgggctt catcgagagc cgccggcggt gccacacgtc gtagccggac
     4141 acctcgcgcg gcagaggcgg ccgcggcccg acgaggctca tctcgccctt gagcacgttc
     4201 cacagctggg gcagctcatc gatgctggtc cgtcgcagcc agccacccgt gctcgacaga
     4261 cgggggtcgt cttgcatctt gaaggcgtgg ccctggatct cgttctgctc gaggagatcg
     4321 ggcagccgtg cctcggcgtc ggggaccatc gatcggaact tcacgacctc gaacgcacga
     4381 ccgtgcaggc cgacccgtgg ctgccggaac aggaccggcc ggccgtcgcg acgaaggatc
     4441 cacagcgcca ccgcgaggag cacggggctc agcaggacga gcgcggtggc ggagccgagg
     4501 atgtccagga cccgcttgcc cacgagcgcg atgatgcgat ccggcccgta caccagggac
     4561 aggatgggga tgccgtcgaa ctcttcctgg tgggcgccgg gaagcgacag catgccgtcg
     4621 aagagcggga cccggacgat cttgccttcc tcctcgcaga gccgggcgat cggctcgatc
     4681 atgccccacg cggccggcgg gaggcagatc gcgacctcgt cgaccacgct ggcgtggagc
     4741 acctgcacga tgtcgtcgac ccggcccagg atgggtcgcg tcaccgtcgg cgcccgcttc
     4801 gacttggtcc agtgcaggtg gccgatcacg cgcaggccca gctcgcggtg ccgctcgatg
     4861 cgacgggcga acgcctcggc gttgccgtcg gtgcccacga tgagcacgaa tcggcggctg
     4921 aagccattgg atcgggctac gcgaaatgcg atccgaaggg cgaatcggat cgcgatcgtc
     4981 acggcgatct gggccgggaa caggacgagc aggaagagcc ggctgacatc cggcatcttc
     5041 aggatgaaca gcgcgctgaa ggtcaggatc gcgagcccga tgccggtgcg gaggacgtcg
     5101 atcgcctcgg agcgcgccga ccagcgcacc cggttccggt acaggccgtt cagccacaac
     5161 gcgacgaccc aggcgagcgc gtaggccgca agcacccata ccggatcgat tccggctttt
     5221 cgccaggagc tcgtccagtt gtcggcgccg aagcgcgtca gcgagagcat gacgaagagg
     5281 ccggccgcgg acatgccgtc cgcgaccatg aggctcaggc gaaacgccat cgtgtatcgg
     5341 cggagcatca gccttcctca gcgagaaccc gacagccccc gaccggtcct gacgatacgc
     5401 caggagcgtc ccgagcgtac gcgggcgacg ttagcgcggg gtttccagcc atggcctgct
     5461 cagcgcgcgc cggcagccct cgcccccgcg gggacggctt ctcgcggcct cccggtgcgc
     5521 tcgtcttcgc cgaggcgacc gatggccagg taccggtatc cggcgcgccg gatgggctcg
     5581 ggctcgagca gccgacggcc gtcgatgatc aatggctggc ggacgatcga ccgcaggccc
     5641 ggccagtcga tctcggcgaa ttccggccac tccgtcgcga tcacgatcgc gtccgcaccc
     5701 cggaccgccg cgtcgacgga cgccgcgacc tcgagcccgg ggagggcgcg ctgcgcgttc
     5761 atcgacgcga cggggtcgta gccgacgacc tcggccccgc cctcgatcaa ccgcgttgcc
     5821 gtgccgaggg ccggggattc gcgcacgtca tcggtgccgg ccttgaacgc gagcccgagg
     5881 agcgcgatcc ggcggccggg caggccgttg agggcgcgct cgatgcgcgc cgcgaaccgc
     5941 gcctgggtgg cctgattggc gttcgacgcc gcggcggtga cgtgcatcgc caggccccgc
     6001 gccagtccgg cggcttcgag gaccttcagc tccttcggca ggcagctgcc gccaaagccg
     6061 aggccggggc gcatgtactg gctgccgatc cgcgggtcgg cggcgatgcc ggccaggacc
     6121 tcgtcgacgt cggcgccgta ctcctcgctc aggctggcga cctcgttcgc gtaggacagc
     6181 ttcagggcga ggaacgcgtt cgacccgttc ttgatcagct cagcggccgc cacgctgacc
     6241 accatccgct cgccgggcag gccgtcgaac agcccgagca ccaggtcgag caccccttga
     6301 tcgacgtcgg ggaaggtgcc gaccacgatc cggctggggt gcaggaagtc gtgcagcgcg
     6361 gtgccctgtc ggaggaactc cgggttagtg acgaggcgcg cggtcctggc ccccgaccac
     6421 tccgcgagcg cgcgcgtcgc gcccggcggc atcgtgctcc gcatgacgag cgggacgccg
     6481 ctctcgacga gcggcccgag atcggcgagt gccgaccgca cctggctcat gtcgctctgg
     6541 ccgtcggcat cgatcggcgt cccgacgcac acgagcgccg cgtcgacgtc ggtcgtcgcc
     6601 ctggcaacga cctcgatcgt gccgttgtcg agccccgcgt cgaaggccgc ctggaggcca
     6661 tcctcgtgaa tcgggatgtg gcgccggcgc agcgcctcga ggcgctcggg attcgtctcg
     6721 acgaagcgaa cggtgtgccc gagcccggcg aggcaagcgc ccgtcacgag tccgacatac
     6781 ccggcgccga tgacggccag gcgacggcgc ggcgagttca cgttcgaatc gtagcaggca
     6841 ggatcagggc ttccccggct tgcgcccggc gatcaacgca tcgatggtca tgagcagccg
     6901 gagccgctgc tccgtccagc tcagcccggc gagcgcctcg acacccgctc tcgcaaacgc
     6961 cgctcggcga tccgcgtcca ccagggacgc gatcgcatcg cggaccgtct ccgccgatgc
     7021 cggactcacg taggtcaccg tgtcggcggg aaggtaggcg cgcaggaccg acgtcgacgt
     7081 ggcgatcgca ggcaccccga gggcgaggta ctcgaggacc ttgaccggca ggatcgaatc
     7141 gacgaactgc gactcgtggt acggcgcaac gccgacgtcg acccccgcga cgaacgcggg
     7201 gatctcgtcc ggcaggaagg tgcgccggtc gaaggagatg acgtcgcgtg cgtcgaggga
     7261 gtcgatcagc tcctcgatcg ccggcgcgta gtcgccgtcg ccgaggatcg acagccgcac
     7321 cggcgttccg gctgcgtgca gggccgcgac cccgcggacg gccagatcga ccccgaaccg
     7381 ctccgcaatc gtgccgtggt ggccgacgcg cagcgccgtt cgtgcgcgcc cgatcggcct
     7441 ggcggcgcgg gcccgcccct cctcgtacca ggcctgctcg tcgggaacgt tgaggacgtc
     7501 gatcacgggc ttgcccggca cgcgctgctg gatctggtcg cgatacggag cgtggacggt
     7561 cacgatcgcc gaggcaaacc ggtacgccgc cgtctcgacc agccggatga gcctggcaac
     7621 gagcctgctg gccgcgccga acttcgcctc gaacagctgg ctggtgatgt cgtggacatc
     7681 gagcacgacc gggatgcgac gcagccgagc cggcagtgca gcgaacacca gcacatccgg
     7741 cggtgcattg acccacacga cagcgagctg cgaaccgggc atcctggcca cttcgatcag
     7801 ggcacgaaac gtaaatcccg cgtatgcgag gacgtaggcc aggcggctcc cgccccggta
     7861 cttcgccgct ccgagcgagc gcaggcgacc gccgtcggcc agcgccgccg cctcggcctc
     7921 gcgtccgccg accccgacga tccgcacgtc gtagccgccc gcgaccaggg cgcggacgtg
     7981 acgccggaca cgggaatcgc gctcgtaccg ctcgtacacc agcatgagca cgatcgggcg
     8041 cggtcgggca gcctgcatgt gcggcgagcc taccgggccg gcggcatcgg ggcacgaacc
     8101 ctgctgtacc gtgtcacgcc gtggtggacc cgagggccga cggaatgccg gggcctgcgg
     8161 ctccccagga cgagcatgcg caggcccgtg gctctgggct cgcgctcgac agcgcgatgt
     8221 cgatggcggc gctcgtcatg ctcggcggaa cgcgggtcgt gtacggctcg atcctgagcc
     8281 acgcgaccga cagggagacg ttcggcgtcg tcggcctgct gatcgggctc acgatgatcg
     8341 gaagcttcct cctgccggcc ggcatcgggt cggcgatctc gcgctacatc ccgttcaacc
     8401 tcggacgagg ggacgcagcg gcggccaggg gtctctatcg gctgctcctc cgggtggcgc
     8461 tggtggcggg cctcccgctg gcgatcgggg cgtttgcgat cgcgcggctg gcccttgacc
     8521 gcccgaccgg ccagagcatc gaggtcgccc tgctcatgct cgtgtacgcc ctgtacacgg
     8581 tctgcaaggc cgggctgtac ggcttcggcc gggtgggctt ctaccttcgg ctcgagatca
     8641 tctcgagcgt cctcgtgctt gcccttgcgc tggccgtcgc ggtgacgggc tcgacgtcct
     8701 acctcgcgcc actgatcgtc ggctacgggg cgttcgcagc cgctgccgcg taccggctcc
     8761 gcacggacgc tgcaggcgca gcgagcgcac cggatcccgg gctcaagcgt gaggtcggcg
     8821 ggttcgtgat cctcgcctgc ctcggcacgc tggcttcggc aggcttcctg cagagcacgc
     8881 agttcctggc ggcgaccttc gcgccagtcg cgcaggttgc ttcgttcgcg gctgcggtca
     8941 ccctggtggc accggtccag ttcctgccgc gcgcgctcgc gctcgccatg ttcccgtgga
     9001 tggccgcggc acacggcgct ggtgacgtgg acgcgatccg acgccaggtc gacctgtcca
     9061 cacgagccct cgtcgtggtg ctcacgccgc cgttcgcggc agggatcatc ctggccccgg
     9121 agatcctcaa ggtgttcggc ggcgaggcat acgtcgacgg cgcccacgtg ctccagctca
     9181 tcctggtggg ggcgttcctg tcggtcatcc aggtcggctc cgtgaacgcg ctgtccagcg
     9241 cgtcggactg gcagctcagg atcccggtcg cgtgggcagt catcggcgca ctcagcggcc
     9301 tggcattcgt cgccctcgcg ggtcccgtct tcggttccac cggggtcgca ggcgggtaca
     9361 tcctgggcac gctcgtctcc gcggcagggc cgatcgccgt cgtctggcgc cgctggagca
     9421 tgccctgggg cggcgtcttc gcgcgtgcga ccatagccat cgccatgtcg ctgatcgttg
     9481 ccgtcctcgt tccctcgcga gccgcggccg cgtcagaggt catcctcgat gtcgtcctcg
     9541 ctgggatcgc ggcctcaatt gccgcggcgg tcctcgcccg cgacatccgg gcgatcgtcg
     9601 tcgcgcgccg gcggccgtag ggtggcttca ccggcgatca ccgaaccccg gcgaaccctc
     9661 ggcgccacgt cgatcggccg gccgggcggg tcgtccctcg tgcgcggcgg cgcatggctg
     9721 ctgctggtgg cggtgttggc cgcggtgacc ctggaaggcc tcgtggcctc gagcctgggt
     9781 ccggccgccg gcgacctgct ccggatcggt cggaacgcgg cctacctcgt cctcgcgccg
     9841 ttcctgctgg ggtacgtggt cctcaccggc cgcatccgag cctttctcca gcccgtcgac
     9901 attgcgctcg ccgtgctcgt cgccgtcatg ttcatcggga gcctggcggc cgattcctcg
     9961 atccggctga cgggcgaagg catgttcgtg tacctccgcg ggacgatcat cttctacgcg
    10021 atccgagccc tgcgcccgag ctgggccgac gcccggcggc tcgcctggct cctcggcgga
    10081 atcttcgtcg tgaacgcgat cgtcgcggtc atccagggat tcgtcggcct gccggcattc
    10141 accttcttcg ggttcacgga cctgacctgg gcggtgatca accgagccca ggggctgcag
    10201 gtccatccga atcacctggg ccacatcctc ggcctcgggt cgatggtcgt gctcggcctc
    10261 atcgcctcga aaccgcgtgt gccggcgcgc tggtgggccc tgttcgccct cttcgcctat
    10321 gccattgcgg caagccagtc gcgcgagacg gtgctcggca tggtggccgg cctcatcgtc
    10381 ctgctcatcc tgcggcggtc cgcgatcaag cggctcgtcg ccgccgggac cgtgctcgtg
    10441 gcgttcgtgg ccgtgatctg gatcgcccac ccggcgaact tcgccgagct gacgcggcgc
    10501 ctgaccggcg tcgtcaccgc gatcgagatc ccgagtggcc aggaggaggg cgtcgtctgc
    10561 gacccgtccg tcgagccgtg cacgatcgaa ggcgtgcccc accgcgaggt acgggtgctg
    10621 tacatccagc agggcctcgc cctgtggacc aggcagccgg tgttcgggta tggcatcggc
    10681 cagttcggcg gggccgtcgc cttcgaggac gacccgcggt ggtacctcga tccgaggttc
    10741 ggcccgaatg gcttcagcat gcacggcttc gaggcgaagc aggtcgacag cttctggctg
    10801 cacctgttcg tcgaggtcgg ggcggctggc tcgatcgcct acgcgatctg gtacctcctc
    10861 ctcggctggc cgctcgcgcg ctggagctgg ccgcgtcgtc gctcggccct ggccgatggc
    10921 gccaccgacc tcgcgctgtc cctgcaaccc gcggccgtcg ccgcgctggc gttcgcgtgg
    10981 gtcgtggcgg tcttctcgcc atcgctggag gacccgatgt tcccaccgct cctgttcgcc
    11041 ctgctcggtt tcgcctggct gactcgaccg gcggacacgt cggaacgccc gatggacccg
    11101 gcgcccgggc cggcctgacg ttgtccgagg ccgctgggct cacccggccg ttgcgcgtgc
    11161 tgttcgtgac caacatgtgg cccgagcacg gcggcttccg cgggacgttc gtcgagcagc
    11221 aggcctcgtc gttgcgcgag ctgggccatc acgtcgacgt ggagatcgtg gcccaggcgc
    11281 gcggcatcgc cgactacgtg ctggctgccc cgcgcgtccg caggcgggct cgcgaaggtc
    11341 gctatgacgt cgtccatgtc catttcggcc tgacggcctt cgccgcgcgc ctcgtcgacc
    11401 ggccgcgagt catcacgctg tacgggagcg acatcaacga gcgcctgaag cgctggtgga
    11461 cccggctgag ctggggcggc gcggcggcga gggtgtacgt gtcgcgcagc ctcgcggcgg
    11521 tggctggaga tcgggccggc cgggtcgtcc cgaacggcgt ggacttcgac ctgttccgac
    11581 ccggcgaacg cacggccgcg cggaccgagc tgggagtcgg gccggacgat cggctgatcc
    11641 tgttcgggtc gcagcccgag cgggcggtca agggccacga cgtgttcgcg gacgtcgtcc
    11701 gccggcttcg ttcgcaaggg atccccgctc gagagctgat cctgacctcc ccgggtcagg
    11761 cggccagccg ggtcgtcgcc aagtacgacg ctgccgacgt gctcctgttc acctcgcgtc
    11821 gcgggagcga gggttccccg acggtcgtca aggaagccat cgcgatgggg ttgccggtgg
    11881 tctcggtcga tgtcggggac gttgcggaga tgctggcagg catcgagccg tcggaggtcg
    11941 tcgccttccc gaaggagggg agcgtgggtg cagcccgcga catgctcgtt gggcgcctcg
    12001 ccgacgcgac ggcccgggtc ctcgcgaacg gggctcgcgc cgacggtcgc gagcgcctct
    12061 cgtggctttc cctggaccag gtggcgggcc gcgtggtcga tgtctatcgc tcggtcgcca
    12121 cgtgaccgtc gcgctggtca ccggcggcct ggggttcgtc ggcgcgcacc tctgcgggct
    12181 gctgcgcgac cggggctggg aggtcgtcgt ggtcacgcgt cgcacggtcg gtgggccgcc
    12241 tggggttcgg ctcgtgagcc tcgacgtcac cgatccccag ctggtcgacg acgcgatcgc
    12301 ggagattcgc ccggacgtca ccttccatct cgccgcagcc atggcccagc gcggcacccc
    12361 ggccgaggcg atcctgagca atgccgtcgt cggcacgctg aacgtgtgct cgagccttcg
    12421 ccgtcgcggc cggccgggca gccggctgat ccttgccggt tcgtcggcgc agtacggcga
    12481 cgtgccgcgc gcggagaacc cggtcgcgga ggacgctccc cagcgcccgg tcaacgccta
    12541 cggcttcgcg aaggtcgccg ccgaggccgt cgcttgcgcc ctggccgccg acggctccct
    12601 tgacgtcatt ccggtccgcg ccttcaacca cgtcggtccg ggtgaatcgg gcaccacggt
    12661 cgccggtgcg ttcgccctgc gcatctcgga cgtgctgcgc ggtgcggcgt cgcgcgtgga
    12721 cgcgggcggc cttgatgcgg tgcgcgattt caccgacgtt cgcgatatcg ctgcgggcta
    12781 tctcgccgcc gccgagcgcg gtcggccggg tcgtccgtat aacctctgct caggccgggc
    12841 gacgagcatc ggcacgatcc tcgatggcct gctccgcgag gcaggcgtcg atcggtcgat
    12901 cgtcgacgag gttccgaccg agcgaggcgg gatcccgttc caggtcggct cgccgatccg
    12961 ggctcgggac gagctgggct gggaggcccg gatcccgctc gatcaaagcc tcgccgacct
    13021 gctcgcggtg acagcactga ggagcgcatg agcggtcgag acgacaccta ctggaacggc
    13081 cggcgggtca gcgtcagcgg ggcgggtggc ttcatcgggt cgcacctcgc ggagcggctc
    13141 gtgcgtgccg gcgcacaggt ccgcgccatg gttcggtaca acgggcggtc ggagctgggc
    13201 tcgctggccc acgccgacgc cgacgtgcgt gcggcgatgg agatcatcca ttccgatgtg
    13261 accgacccat acgcggtccg ccggttcgtc gagggatcgg acaccgtgtt ccacctggcc
    13321 gcgctcatcg cgatcccgta cagctacgcg gcgcccgcgt cgtacgtcgc gaccaatgtg
    13381 tccggcacgc tgcacatgct cgaggcggtg cgcgacctcg gcatcgcgca catggtgcag
    13441 acctcgacgt cggaaacgta cggcactgcc cggaccacgc cgatcgacga ggaccatcca
    13501 ctccagcccc agtctccgta cgccgcatcg aaggtcggcg cggacgccct ggccgaatcg
    13561 ttccaccgcg ccttcgacgt cccggtcgcg acgatccggc cgttcaacac attcgggcct
    13621 cggcagagcg cgcgcgcggt cgtgcccacg atcgcgtccc aggtgcttgc gggccggtcg
    13681 gagatccggc tcggcgacct gcgaccgcga cgcgacctca cctacgtcgc cgacaccgtc
    13741 gagggcttcc tcgccgtcgc ccgggctccc gcgtgcgtgg gcatggtcac caacatcggg
    13801 tcgggcacgg ccgtgtcgat cggcgagctg gcggcactga tcgccgagct cgcgggaaag
    13861 ccggggctgc cgatcgtcac cgacgcggtc cgggtccgac cggagggcag cgaggtcttc
    13921 gagctcgtcg ccgacgccca cagggcggcc gagctcaccg gctggcgccc ggaggtgtcg
    13981 ctgcgagacg gcctccgcca ggccatcgag ttcgtccgca accatcccga cgcgtacgac
    14041 ccggaccggt acgcaacgtg aaggcggccg tcctcgcggg cgggaagggc acccgcctcc
    14101 gaccgttcac cacgacgttc ccgaagccgc tcatgccgat gggcgagatg cccatcctcg
    14161 agctcctgct gcgccagctc cgggcgcacg gcgtcgacga ggtgacgctg ctgacgggtc
    14221 acctcgccta cctgatcgag ggctacatcg gccagggtga atcgctcggc ctgtcggtcg
    14281 actacgtgca cgaggcagag ccgctgggca ccgcgggtcc actccggagc ctggtcggcc
    14341 gcatgacgga tgacttcctc gtgatgaacg gtgacctgct cacggacgtc gacttcacgg
    14401 cgctgatgga ccaccatcgc gcgagccgcg cgaccgtcac gatcagcacg ttcaatcgcg
    14461 aggagcggct cgagctcggc gtcctgcagt ccgcccccga cggcgccatc accggctacg
    14521 acgagaagcc gcagctcaac ctgacggtgt ccatgggcct gtacgcgatg tcgcccgcag
    14581 cgcttcgcct gatcccggac ggctactacg acatgcccga cctgatccgc gatctcctcg
    14641 ccgcgggcca gcccgtcatc ggccggcggc acgagggcac gtggtacgac atcggacgcc
    14701 ccgacgacta cgccgccgcc aacgagctgt tcgcgcgcga cccgcgggcg ttcctcgcgc
    14761 cggagcgctg atcctgccgt gagcggatcg gccgcttcgc gcgtcgcggt cctcttcggg
    14821 acccgtccgg aggcgatcaa gctggcgccc gtgatcctgg ccctgcgcag cgcgggcctc
    14881 gaggtgaccg tcgtcgcgac gggtcagcat cgcgagctcg cgtcggaggt gctcccgcag
    14941 ttcgcgctcg acatcgacgt cgacctcgcg ctcatgcggc cgggccagag cctcgactac
    15001 gtgctgggcg aaggcatcca ggccgtgggt cgctacctgg tcgaggcgaa gccggacgcc
    15061 gtcatcgtcc agggcgacac gagcagcacc ctcgcggcag ccctggctgc cttccaccat
    15121 cgcgtgccgc tggcccatgt cgaagccggc cttcgcaccc acgatctcgg cttcccgttc
    15181 cccgaggaga tgaaccggcg ggtgacgagc gtggtcgcac ggtggcattt cgcgccgacc
    15241 caacgggcag cagagcatct ggccgcggag ggcatcgtcg atcgggtcta cgtgaccggc
    15301 aacacggtcg tcgacgccgc gcgacacgtc ctcgcggcgg ccgcgttgcc cgcggagctc
    15361 gccgaccttg ggaacggcgc ccgctacatc gtcgcgacgg cccaccgccg ggagtcctgg
    15421 gacggcggca tcgagcgcat cgcgcgagcc ctgcgaacca cgctggcggc cgtcgacgac
    15481 atccagctcg tgttcgtcac gcacccgaac ccgctcgccg ccggtcccgt gacggcgatt
    15541 ctccaggggg aggccagggc gcgcgtcgtc acggccatcc cgtacccgag cttcctggcg
    15601 ctgctgcgcg gcgccgtcgt cgcggtcagc gactcaggtg gagtgcagga ggagggcgcg
    15661 accctgggcg ttc
//
LOCUS       DATBSW010000252         9334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_376957, whole genome shotgun sequence.
ACCESSION   DATBSW010000252 DATBSW010000000
VERSION     DATBSW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376957"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VIF63_07035"
     CDS             complement(<1..654)
                     /locus_tag="VIF63_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8239173.1"
                     /translation="MQINGAPFHPSLATSDIARAKAWYADHLGWQPAREFDDLLVYQV
                     GESFFTVFTTEFAGTARNTVMNYVVADVRATVGLLRGRGVAFEDYDFGDFRTVDGVME
                     DPGGGLTAWFKDADGNIVAILQDASVAAPGAIAGMIAAADLGRAKAWYAAKLGLEPFQ
                     EFEGFVLDYKSGDSRFNVYKTEFAGSAKNTVGLWRLKGMREEVARLRGRGVVFEEYDF
                     "
     gene            complement(701..1606)
                     /locus_tag="VIF63_07040"
     CDS             complement(701..1606)
                     /locus_tag="VIF63_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239174.1"
                     /translation="MTPEPTVRCVAGSDEAELDLLTARGHFTIELLPMPLPAPELVDA
                     SIVPVEGILVGGAQLTAEVQGDFSSSDAAFTAVTADFVPFGTTAAIPLDATFEGSMIG
                     VGLQDSAVSGRLRIFVAWTTPCGSREGSGEIGLTIVNSSVVAGCPTTGAGMEDAVAAL
                     QDAHVTVGTAELPFTVVAASARWFGGNIDSLVPQFSGWDRGLSVVVAPATSLLLKKAI
                     EDLSAISIRTTIYKRADVIEYLSPDSTIDLRSVSVTNRTPGPKGNVGLPVPDATGSYV
                     YEIQGQWQTSCINVETYMVVSVEVR"
     gene            complement(1748..2902)
                     /locus_tag="VIF63_07045"
     CDS             complement(1748..2902)
                     /locus_tag="VIF63_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239175.1"
                     /translation="MTDHRRLSSIALAALMLGGCGTDVQSPSPSPPGSTAVATPGPTR
                     TPVAPPAPSLPTGCSGIDKVDLAGIEVTFGSVDEDGNGGEIGFSLGPTSGEMQVTRLT
                     DRAGQERCSTPPSPELLDQRGQILGGHEFITYPSTSFAGHSNPQAMLAAKVTLTLDGG
                     AALDLPTRFIPGNENFDQVAITVPDVAGPGVLRLKFVWADRSFRYEGSSTIAVNVVPL
                     SATEVCVLDSSGYFEQIGDLLRHSIEVNSVSQHAFSPFNTSKFAPFENPGIDAIIVYG
                     FDPDKPSITAEAGTALRIERVSDELTLGNEMNLWVWTRPSVAKAIKDYPPEGSVLVLS
                     RTPVKHADGTFRLRVPQDPGRYVAGVELTYDSQCSSGTLWFVVNIDVVAS"
     gene            complement(2946..4157)
                     /locus_tag="VIF63_07050"
     CDS             complement(2946..4157)
                     /locus_tag="VIF63_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8239176.1"
                     /translation="MAEAALGGLTAEERLLRDTAREFAQREVEPTAIERDEQERFDRS
                     IFTRMGELGLTGAPLPESIGGGGFSYLGWALVMEEIGAADMASAVTLSVHLLSQFPVL
                     NFGTDEQVGRWLPPMLAGEALGAFCLTETSGGSDAASLRTRARRFGADGRELGADADA
                     TDVAEYRLDGTKIWISNAPEADRYLVFATVDPAKGSKGITAFLVEKGTPGFRFGAHEK
                     KMGIRACPAAELIFDGAIVPAANRLGDEGGGYRIALSSLAEGRISIGAGCVGIARSAL
                     EQSAAYLQQRKAFGAPLSELQGLKFMLAEMARDVAAARALVHEAARAKDRGENIAEAS
                     SLAKWTASDTAMKVATDAVQLFGGSGYSRETGIERLMRDAKGAQIYEGTNQIHRLIVA
                     DAVLDRAKAAG"
     gene            complement(4150..5013)
                     /locus_tag="VIF63_07055"
     CDS             complement(4150..5013)
                     /locus_tag="VIF63_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8239177.1"
                     /translation="MAIERVFVAGAGLMGHGIGQVHAAIGKHVVLYEPDLARAQAGRE
                     RIAGNLDRAVAKGKLDSDQRDAIIGRVLASDDLELVRSSDLVVEAVFEDLEVKRALWL
                     QLDEHAPEATIFASNTSSISIDALAEAVAPARRERFVGMHFFSPVPVMPLVELIRGSA
                     TTDATEAAIRELSEALGKQLIVSADRPGFIVNRILMPFLAESMRAYEEGVGTAEDIDT
                     GARVGLNHPMGPLALADFIGLDVCLNVMNVLHEGIGGDHFAPPKVLRDLVAAGHLGQK
                     TGRGFHTYPRG"
     gene            complement(5034..6206)
                     /locus_tag="VIF63_07060"
     CDS             complement(5034..6206)
                     /locus_tag="VIF63_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239178.1"
                     /translation="MTTTDSNARLMTPAFAALSAAVLAFFVAGGIFLPAIPRFTIGPI
                     GGDGIAVGIVVGAFSISSVLMRPFAGRLADRRGRRIGLLAGAAVTVLATFGHLIANDI
                     PVLLLMRLLLGAGEALFFVAAIAAATDLAPESRRGEAISLISLSLYLGVAIGPVIAEN
                     VLAASGFTAVWLVAGAISLAALVLSWIAPETLSATQRAEHPPNSRLIDRRGVTAGLLA
                     LCGVWGMGPFFAFLPLMTDDLGTGASTYFGVFAIVVVVLRVLGAKLPDRIGAARLSGT
                     ALIFSAVGMAISGLAPTEIGLLIGSAIFAIGVAFTFPAIMAMAVVGVPQGERGVVVGT
                     ASLFVDVAFGISPAMLGFLAQWTGYPPTFLVSAVIAAIGAAWLLIRRPGQVVAAPA"
     gene            complement(6203..7183)
                     /gene="meaB"
                     /locus_tag="VIF63_07065"
     CDS             complement(6203..7183)
                     /gene="meaB"
                     /locus_tag="VIF63_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY8239179.1"
                     /translation="MAARATSADALVDAALAGDRRSLARILTAVENRTPVAEIALRRM
                     YPLAGRAHLVGITGPPGAGKSTLVSALIGAVREAGRAVGVIAVDPSSPITGGALLGDR
                     VRMQSYATDRDVFIRSMAARGHAGGLASTSTAAAAVLDAAGFDLVLIETVGTGQSEVE
                     VAAAADTTVVLEAPEMGDEIQAIKAGLLEVADIVVVNKGDRPGAQRTASQLRAMLVAV
                     GRAGGAGASDRPRPKEPEVLLTTATTGAGIPELIKSLDRHRARGREAGDTPARRARAE
                     AQVWAILADRLRADLHAPANRELTAKTMRAVAAHELDPFAAADRLLAKLR"
     gene            complement(7185..7589)
                     /locus_tag="VIF63_07070"
     CDS             complement(7185..7589)
                     /locus_tag="VIF63_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY8239180.1"
                     /translation="MTDRPLKVLIAKPGLDGHDRGAKVLARGLRDEGFEVVYTGLRQT
                     PDMVVTAALQEDADVVGLSILSGAHMTLVPKVTAKLKEAGMDDVLVVVGGIIPDDDVK
                     ALKDAGVAASFGPGTTIAEVAEYLRANARPRD"
     gene            complement(7586..8050)
                     /locus_tag="VIF63_07075"
     CDS             complement(7586..8050)
                     /locus_tag="VIF63_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8239181.1"
                     /translation="MQTTTAGATSRTTIERMDEAWAAFHDRLQAVPAEHLDRHIGDEG
                     WTRKQMLAHIAAWHDITADRLIQFAESDEPGSDPEPEDVVNARAARGAVGRTTGEVVM
                     AMDDSFRRLRREVARLTDAQMAAHDDWASAIIVVNTIDHYTDHLEDLGATHT"
     gene            complement(8061..8750)
                     /gene="folE"
                     /locus_tag="VIF63_07080"
     CDS             complement(8061..8750)
                     /gene="folE"
                     /locus_tag="VIF63_07080"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8239182.1"
                     /translation="MPSPSDLPSHVRAIVDEIENEIGDDPGEAETLIEGVGRHPDNGR
                     VEAAVREILVEIGEDPDRQGLLGTPERVHRMYTELTAGYHVDPERLINGAVFDVTYSE
                     MVIVKDIPFYSLCEHHLLPFFGTAAVAYIPRGKVIGLSKIPRIVEMYARRLQVQERMT
                     QQIADFLNERLAPQGVGVVVEANHLCAVMRGIRKPGTIMTTSAVLGLFRSRDRTRAEF
                     FSHLERRPPSP"
     gene            8811..9269
                     /locus_tag="VIF63_07085"
     CDS             8811..9269
                     /locus_tag="VIF63_07085"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8239183.1"
                     /translation="MVHPRVDQLRFARSEWQRGLRGLREEDAQQRLLPMNSISWMVGH
                     LAWHERLVWAERGQGLRIEPGLDLVANGAPASTPVLADMWAAWRRVLEVADPLLDTLT
                     TTMLEVPLPHDGRSAAPAAGSQLQRITYHYWSHIGEASAVRQILGHKRLA"
BASE COUNT         1448 a         3251 c         3229 g         1406 t
ORIGIN      
        1 gaagtcgtac tcctcgaaca cgacgccacg gcctcgcagc cgcgccacct cttcgcgcat
       61 gcccttgagc cgccacaggc cgaccgtgtt cttggcggag cccgcgaatt cggtcttgta
      121 gacgttgaag cgcgagtcgc ccgacttgta gtcgaggacg aagccctcga attcctggaa
      181 cggctcgagc ccgagcttgg ccgcgtacca cgccttcgct cgcccgaggt cggccgccgc
      241 gatcatgccg gcgatggcgc cgggggccgc gacactcgcg tcctggagga tcgccacgat
      301 gttgccgtcg gcatccttga accatgcggt cagcccaccg cccgggtcct ccatgacccc
      361 atcgaccgtc ctgaagtcgc cgaagtcgta gtcctcgaat gcgacgccgc gaccgcgcag
      421 gagcccgacc gtcgcccgaa cgtcggccac gacgtagttc atgaccgtgt tcctggccgt
      481 cccggcgaac tccgtggtga acaccgtgaa gaacgactcg ccaacctggt agacgagcag
      541 gtcgtcgaat tcgcgggccg gctgccagcc caggtggtcg gcgtaccacg ccttcgcgcg
      601 agcgatgtcg gatgtggcca ggctggggtg gaacggggct ccgttgatct gcatggggac
      661 ctccgcgccg aggctacacc cgatcccggt ccgaggccgg tcagcgcacc tcgaccgaga
      721 ccaccatgta ggtctcgacg ttgatgcacg acgtctgcca ctggccctgg atctcgtaga
      781 cgtagctgcc cgtcgcgtcc gggacgggaa gccccacgtt gcccttcggg ccgggcgtcc
      841 ggttggtgac cgaaaccgac cgcaggtcga tcgtcgagtc cggcgacagg tactcgataa
      901 cgtcggcgcg cttgtagatc gtcgtgcgaa tcgagatcgc cgacaggtcc tcgatcgctt
      961 tcttgagcag gagcgaggtc gcaggcgcga cgaccaccga caggccgcga tcccagcccg
     1021 agaactgcgg gacgaggctg tcgatgttgc ccccgaacca gcgagcgctg gcggcgacca
     1081 ccgtgaacgg cagctcggcg gtcccaacgg tcacgtgcgc gtcctggagc gccgcgaccg
     1141 cgtcttccat ccctgcgcct gtggttgggc agcccgcgac cacgctcgag ttgacgatcg
     1201 tcagtcctat ttcgccggag ccttcgcgac tcccgcacgg cgttgtccac gcgacgaaga
     1261 ttcggagccg gccactcacg gcggagtcct gtagccccac gccgatcatc gaaccttcga
     1321 aggtcgcgtc gagcgggatc gcggcggtcg tgccgaaggg cacgaaatcg gccgtgacag
     1381 ccgtgaacgc tgcgtcgctc gatgagaagt cgccctgcac ctcagccgtg agctgggcgc
     1441 cgccgacgag aatcccctcg accggcacga tcgacgcatc gacgagctcg ggcgccggga
     1501 gcggcatcgg caggagctcg atcgtgaaat ggcctcgtgc cgtcagcagg tcgagctccg
     1561 cctcgtccga tccggcaaca cagcgcaccg tcggctcggg cgtcacggca accgtagcga
     1621 tcggcgacgg cgagacaatg ggcgccgcgc tgggcggggt cgtcgttgtg ggcgctgtgg
     1681 gtgcccgacc atcgcatccg gccaccacga acgccagtgc gaggccgacc aattgaccgc
     1741 ggcgcatcta cgaagcgacc acgtcgatgt tgacgacgaa ccacagtgtg cccgacgagc
     1801 actgcgagtc gtaggtgagc tcgacaccgg cgacgtagcg gcccggatcc tgcgggaccc
     1861 gaaggcggaa cgtgccgtcg gcgtgcttca ccggcgtccg cgacagcacg agaaccgatc
     1921 cttcgggtgg gtagtccttg atggccttcg cgacgctcgg tcgagtccac acccagaggt
     1981 tcatctcgtt cccgagcgtg agctcgtccg atacgcgctc gatccgaagg gccgtgccgg
     2041 cctcggccgt gatcgaaggc ttgtcggggt cgaagccata cacgatgatc gcatcgattc
     2101 ctgggttctc aaacggggcg aacttcgacg tattgaacgg cgagaaggca tgctgcgaca
     2161 ccgagttgac ctcaatggag tgacgcagca ggtcaccgat ctgttcgaag tagcccgacg
     2221 agtcgagcac gcaaacctcg gtcgccgaga gcggcaccac gttcacggcg atcgtactcg
     2281 agccctcata ccggaacgac ctgtccgccc atacgaactt gaggcggagg actcccggac
     2341 cggcgacgtc ggggaccgtg atcgcgacct ggtcgaagtt ctcgttgccc gggatgaacc
     2401 gcgtcggcag atcaagcgcc gcgccaccgt cgagtgtcag ggtcaccttg gccgccagca
     2461 tcgcctgcgg attgctgtga ccagcgaagg acgtcgatgg gtacgtgatg aactcgtgcc
     2521 cgccaaggat ctggccccgc tggtcgagga gctccggcga tggcggtgtc gagcagcgtt
     2581 cttgacccgc gcggtcggtc aggcgggtga cctgcatctc gccagacgtc ggaccgaggc
     2641 tgaagccgat ttccccaccg ttgccatcct catcgacgct cccgaaggtc acttcgatac
     2701 cggcgaggtc gaccttgtcg atgccggagc agccggtcgg gagactgggc gccggcggcg
     2761 ccacaggtgt gcgcgtcggg cctggcgtcg ccacggctgt tgatccgggc gggctcggcg
     2821 atggcgactg aacgtcggtg ccgcaaccac ccagcatgag cgcggcgagc gcgatgctcg
     2881 acagtcggcg gtggtcggtc acggcgccaa catgacgagg gcgtcgattt ggatgtaacg
     2941 gggcctcagc cggcagcctt cgctcgatcg aggaccgcgt cggccacgat caagcggtgg
     3001 atctggttcg tgccctcgta gatctgggcg cccttcgcgt cgcgcatgag gcgttcgatg
     3061 cctgtctcgc gtgagtagcc gctcccgcca aagagttgga cggcgtcggt cgcgaccttc
     3121 atcgccgtgt cgctggcggt ccacttggcg agggatgacg cctcggcgat gttctcgccg
     3181 cggtccttgg cgcgcgccgc ttcgtgcacc agcgccctcg cggctgcgac gtcgcgggcc
     3241 atctcggcga gcatgaactt caggccctgc agctccgaca gcggcgcccc gaatgccttt
     3301 cgctgctgga ggtacgcggc cgattgctcg agcgcggagc gggcaatccc gacgcagccg
     3361 gcgccgatcg agatccggcc ctcggccagg ctcgagagcg cgattcggta tccgccaccc
     3421 tcgtcgccca gccggttcgc ggccggaacg atcgcgccgt cgaagatcag ctcggcagcg
     3481 ggacaggctc gaatgcccat cttcttctcg tgcgccccga accggaagcc gggcgtgccc
     3541 ttctcgacca ggaacgcggt gatgcccttc gagcccttcg cggggtccac cgtcgcgaac
     3601 acgaggtagc gatccgcctc gggagcgttc gagatccaga tcttcgtacc gtcgaggcga
     3661 tactcggcga cgtcggtcgc gtccgcatcg gcacccagct cgcggccatc ggcgccgaac
     3721 ctgcgggccc gcgtccgcag cgatgcggcg tcgctgccgc ccgaggtttc ggtcaggcag
     3781 aacgcgccga gcgcctcgcc ggcgagcatc ggtggcagcc accgcccgac ctgctcgtcc
     3841 gtgccgaaat tcaggaccgg gaactgcgac aggaggtgca ccgagagcgt cacggcgctc
     3901 gccatgtcgg cggcgccgat ctcctccatc acgagcgccc agcccaggta gctgaacccg
     3961 cccccaccga tcgactcggg cagcggcgcg ccggtcagcc cgagctcgcc catccgggtg
     4021 aagatggagc ggtcgaaccg ctcctgctcg tcgcgctcga tcgcggtggg ttcgacttcg
     4081 cgctgggcga actcgcgggc cgtgtcccga agcagccgct cctcggcggt caggccgccg
     4141 agcgccgcct cagccacgcg gatacgtgtg gaaaccgcgg cccgtcttct gcccgaggtg
     4201 gcccgccgcg accaggtcgc ggagcacctt cggcggagcg aagtggtcgc cgccgatccc
     4261 ctcgtggagt acgttcatca cgttgaggca cacgtcgagg ccgatgaaat cggcgagggc
     4321 gagcggaccc atcggatggt tcagccccac cctcgcgccc gtgtcgatgt cctcggccgt
     4381 gccgacgccc tcctcataag cccgcatcga ctcggcgagg aacggcatca ggatgcggtt
     4441 cacgatgaac cccggccggt cggccgagac gatgagctgc ttgccgagcg cctcgctgag
     4501 ctcgcggatc gcggcctcgg tcgcatcggt cgtcgccgag ccgcggatga gctcgacgag
     4561 tggcatcacc ggcaccgggc tgaagaagtg catgccgacg aagcgctcgc gccgtgccgg
     4621 tgcgacggcc tcggcgaggg catcgatcga gatcgagctc gtgttcgagg cgaagatcgt
     4681 cgcctcgggc gcgtgttcgt cgagctgcaa ccacaatgcg cgcttgacct cgaggtcctc
     4741 gaagaccgcc tcgacgacga ggtcggacga gcgcaccagc tcgaggtcat cggaggccag
     4801 cacgcgaccg atgatcgcgt cccgctggtc ggaatcgagc ttgcccttgg ccacggctcg
     4861 atcgagattg ccggcgattc gctcgcgacc cgcctgagcc cgggcgagat ccggctcgta
     4921 cagcacgacg tgcttcccga ttgcggcgtg gacctggccg atgccgtggc ccatgagccc
     4981 ggcgcccgcg acgaagaccc gctcgatcgc catcgaccga agggtacccg ccgctacgcg
     5041 ggcgccgcga ccacctgccc cggccggcgg atcagcagcc acgccgcccc gatcgccgcg
     5101 atcacggctg acacgaggaa tgtcggcggg tagccggtcc attgggccag gaaaccgagc
     5161 attgccggcg agatcccgaa cgcgacgtcg acgaacaggc tggccgtccc gacgacgacc
     5221 ccgcgctccc cctgcggcac gccgaccacg gccatggcca tgatcgccgg gaaggtgaac
     5281 gccaccccga tcgcgaagat cgccgagccg atgagcaggc cgatctcggt cggcgcgagg
     5341 ccgctgatgg ccataccgac tgcggagaag atcagcgccg tgccggacag cctggctgcg
     5401 ccgatgcggt caggcagctt cgccccgagc acgcggagga ccacgacgac gatggcgaac
     5461 acgccgaagt aggtgctggc gcccgtccca aggtcgtcgg tcatgagcgg caggaacgcg
     5521 aagaacggac ccatgcccca cacgccgcac agcgcgagga ggccggccgt gaccccgcgc
     5581 cggtcaatga ggcgcgagtt cggcggatgc tccgcgcgct gcgttgccga cagggtctcg
     5641 ggcgcgatcc agctcagcac gagggcggcg agggagatcg ccccggcgac cagccacacc
     5701 gcggtgaagc cgctcgctgc gagcacgttc tccgcgatga ctggcccgat ggcgacgccg
     5761 aggtagagcg agagcgagat caggctgatt gcctcgcctc ggcggctctc gggcgccagg
     5821 tccgtggcgg cggcgatcgc ggccacgaag aacagcgctt cgcccgcgcc gaggaggagc
     5881 cgcatcaaga gcaggaccgg aatgtcgttc gcgatgagat gtccgaaggt cgcaaggact
     5941 gtgaccgcgg ccccggcgag cagtccaatc cggcgccctc ggcggtcggc gagccgcccg
     6001 gcgaagggcc gcatcagcac cgacgagatg ctgaacgcac cgacaacgat gccgacggcg
     6061 atcccgtcgc cgccgatcgg cccgatcgtg aatcgcggga tggcaggcaa gaagatcccg
     6121 ccggccacga agaacgccag gaccgccgcc gacagcgccg cgaaggcggg cgtcatcagg
     6181 cgggcgttgg agtcggtcgt ggtcaccgca gcttggcgag caggcggtcg gcggccgcga
     6241 agggatccag ctcgtgcgcg gccacggctc gcatcgtctt ggccgtcaac tcgcgattcg
     6301 ccggggcgtg caggtcggcg cgcagcctgt cggcgaggat cgcccagacc tgcgcctcgg
     6361 cgcgcgcgcg ccgggccggg gtgtcgccgg cttcgcgacc gcgcgcgcgg tgccggtcga
     6421 gggacttgat cagctccggg atgccggcgc cggtcgtggc cgtcgtcagc aggacctccg
     6481 gctcttttgg tcgcgggcgg tcactcgctc cggcgccccc ggctcggccc accgcgacga
     6541 gcatcgcccg cagttgcgaa gcggtccgct gggctccggg ccggtcgccc ttgttcacga
     6601 cgacgatgtc ggcgacctcg agcagccccg ccttgatcgc ctggatctcg tcgcccatct
     6661 cgggcgcctc gagcaccacg gtcgtgtcgg ccgccgcggc gacctccacc tcggactgcc
     6721 cggtcccgac cgtctcgatc agcaccaggt cgaagcccgc cgcgtcgagg acggccgcgg
     6781 cggccgtcga ggtcgaggcc aacccgcccg cgtggccgcg tgccgccatc gagcggatga
     6841 acacgtcgcg gtccgtcgca tagctctgca tccggacgcg atcgccgagc agggcgccgc
     6901 cggtgatcgg gctcgagggg tcgaccgcga tcacgccgac cgcgcgacca gcctcgcgaa
     6961 ccgcgccgat cagcgccgag acgagcgtgg acttgcctgc gccgggcggc ccggtgatgc
     7021 ccacgaggtg cgctcgaccg gcgagcgggt acatgcgccg cagcgcgatc tccgccacgg
     7081 gcgtgcgatt ctcgacggcc gtcaggatcc gggcgagcga tcgacgatcc cctgccaggg
     7141 ccgcgtcgac gagtgcgtcg gccgaggtcg ctcgcgcggc cacctcagtc gcgagggcgg
     7201 gcgttggccc tgaggtactc ggcaacctcg gcgatcgtgg tgcccggccc gaacgacgcg
     7261 gcaacgccgg catccttgag cgccttcacg tcatcgtcgg gaatgatccc gcccaccacc
     7321 acgagcacgt catccatgcc cgcttccttg agcttcgcgg tgaccttggg cacgagcgtc
     7381 atgtgcgcac cggacaggat cgaaagcccg acgacgtcgg cgtcctcctg gagcgcggcc
     7441 gtcacgacca tgtccggcgt ctggcggagg cccgtgtaga cgacctcgaa gccctcgtcg
     7501 cgcaagccgc gggcaagcac cttggcaccg cggtcgtggc catcgagacc cggcttggcg
     7561 atgagcacct tgagcgggcg gtcggtcaag tgtgggtggc ccccaggtcc tcgagatggt
     7621 cggtgtagtg atcgatcgtg ttgaccacga tgatcgccga cgcccagtcg tcgtgcgcgg
     7681 ccatctgggc gtccgtcagc cgagcgacct cgcgtcggag ccgccggaac gagtcgtcca
     7741 tcgccatcac gacctcgccg gtcgtgcggc ccacggcgcc gcgtgctgcc cgggcgttga
     7801 ccacgtcctc gggctcgggg tccgatccag gctcgtccga ctcggcaaac tggatcagcc
     7861 ggtccgccgt gatgtcatgc cacgccgcga tgtgcgcgag catctgcttg cgcgtccagc
     7921 cctcgtcccc gatgtgccga tcgaggtgct cggcggggac cgcctgcaat cgatcgtgga
     7981 aggcggccca cgcctcgtcc atgcgctcga tcgtggttcg gctggtggcg ccggcggtcg
     8041 tcgtctgcat gtcggttacc tcacgggctc ggtggacgtc gctcgaggtg gctgaagaac
     8101 tctgcgcgcg tccggtcgcg ggatcggaac aggccaagga cggccgaggt cgtcatgatc
     8161 gtgccgggct tgcggatgcc ccgcatcacg gcacacaggt ggttcgcctc gacgaccacg
     8221 ccgaccccct gcggcgcaag tcgctcgttc aggaagtcgg cgatctgctg agtcatccgc
     8281 tcctggacct gcagcctgcg ggcgtacatc tcgacgatcc ggggaatctt cgacaggccg
     8341 atgaccttgc cgcgcgggat gtacgcgacg gcggcggtcc cgaagaacgg cagcaggtgg
     8401 tgctcgcaca gcgagtagaa cgggatgtcc ttgacgatga ccatctcgct gtacgtgacg
     8461 tcgaagaccg cgccgttgat cagccgctcg gggtcgacgt ggtagccggc ggtcagctcg
     8521 gtgtacatcc gatgcacacg ttccggggtg ccgagcaggc cctgccggtc ggggtcctcc
     8581 ccgatctcga cgaggatctc gcggacggcg gcctcgacgc gaccgttgtc ggggtggcgt
     8641 ccgacgcctt cgatcagggt ctcggcctca ccgggatcgt cgccgatctc gttctcgatc
     8701 tcatcgacga tggcgcgcac gtggctgggc aggtcgctcg ggctgggcat cgacggaagg
     8761 gcctcctcgc agggcgcgat ggtacgtcgg acgctgctag gctgcgaccg atggtccatc
     8821 cgcgggtcga tcagctccgt ttcgcgcgat ccgagtggca gcgggggctg cgtgggctgc
     8881 gcgaggaaga cgcgcagcag cgactcctgc ccatgaactc catctcgtgg atggtcggcc
     8941 atctggcctg gcacgagcgc ctggtgtggg cggagcgcgg gcagggcctc cggatcgagc
     9001 cgggtctcga tctcgtggcc aacggcgctc ctgcctcgac gcccgtcctg gccgacatgt
     9061 gggcggcctg gcggcgcgtc ctcgaggtcg ccgatccgtt actggacacg ctcacgacga
     9121 cgatgctcga ggtgccgctc ccgcacgacg gccggtcggc cgccccggca gccgggtcgc
     9181 agctccagcg gatcacctac cactactggt cgcacatcgg ggaggcgtcg gcggtccgcc
     9241 agatcctcgg ccacaagcgg ctggcctagt tcgtcggcga catcgagggc caggcgccgt
     9301 accgcccgga gggctgacgc caggggctca gccc
//
LOCUS       DATBSW010000253        12598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_518259, whole genome shotgun sequence.
ACCESSION   DATBSW010000253 DATBSW010000000
VERSION     DATBSW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518259"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VIF63_07090"
     CDS             complement(<1..865)
                     /locus_tag="VIF63_07090"
                     /inference="COORDINATES: protein motif:HMM:NF021608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY8239184.1"
                     /translation="MSENRSSRAPGRRPQGWRADRRWQAGFILGSAIGAAATVLGRRA
                     ERIARRGLVDWGAVERLAIRRAERAPGRLSPLELRAADGAYREAMARVVPRLSEALGT
                     SLPGVVERASVVDRAGWIRANVGTFRSLISRMEQDLLDQVVPEGGGLTRATMALANRW
                     VTSQQLGFLLGFMGTRVLGQYDLAMLSAEAEDGRLLFVEENIRATAHSLGIPVAPFRT
                     WIALHETTHAFEFEAHPWLRPYIAERLERQLSLFSGNASSLGRDTLRAIGRALRGDST
                     GEHWMETIMGEE"
     gene            complement(862..1725)
                     /gene="murI"
                     /locus_tag="VIF63_07095"
     CDS             complement(862..1725)
                     /gene="murI"
                     /locus_tag="VIF63_07095"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY8239185.1"
                     /translation="MTDPRPIGVFDSGVGGLTVLREVLRRTPDESTIYFGDNARAPYG
                     VRPDAEVRTFSIESIDALVARDVKAVVVACNTSTAIALADLRRRYDLPILGVIRPGAT
                     SAALASRNRRVGVIGTPATIRSHAYFSAIKDENPAVEVYEHATPTFVPMVEAGLLTGA
                     EVEASVREALAPLLGERDAAGEFVFPLPASAKVDTLLLGCTHYPLLRAAIAAVAGDGI
                     AIVDSAAATASALAELLSINGLDAAPGGATHLQLTTGDVDAFRSIAERMFGEAFPDVA
                     AIDLAAMGSAT"
     gene            complement(1762..2928)
                     /locus_tag="VIF63_07100"
     CDS             complement(1762..2928)
                     /locus_tag="VIF63_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8239186.1"
                     /translation="MASGDQIRLLLVEDVPQVAQYVRGLLNAQSQVRLVDIVSDGTMV
                     PMRISEHRPDVIVVDALLQGRLRGMQLVKQLHDSKTGVPVIVLTVPQNPVTVEPEKGI
                     DEVLSMPFNGYDLITRIAGVHKTATAATERGPSRLVVVFAPKGGVGKSTIAFNLGVAA
                     SGLGLKVALVDGSIQFGDLRGLLRAPREAPSMLDLPTDRISELDLADVLWRGPADVDV
                     LLAPPRIEMAEMVLIRDLEKTLSLMRRLYDIVIVDTGVGLDELTLALLDQADTILEIV
                     TYDATTIRNTIAMAETFQKIGYPPSKVKYLVNRADASAGIDPNDLRNALGREPEFKIR
                     SEGNVVGPASNQGAPFVTAEPAAGVSKDLIEVAIQMFGKAAAGARSDRQLAAAR"
     gene            complement(2971..3786)
                     /locus_tag="VIF63_07105"
     CDS             complement(2971..3786)
                     /locus_tag="VIF63_07105"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8239187.1"
                     /translation="MTRVADETARAAAIARLYDLDLSVDAGDVELFQALARRTGGPIV
                     EFAVGSGRIAVPLAADGHRVVGVDIEPAMLARARARIAQAPKGVADRLELIEADMTTA
                     GTHPEVLARGPYELAILALNSILIMSTPARQRAVFASMARLLAPGGLAVVDTWLPVPS
                     DLTAFDGRLSLDWLRTDPETGFEVTKTSAAWFDPTLRIVTLTTVFEEGAPGTAPIRWT
                     RADALRLISADELVDYATDAGLQVEQLAGDHDLSPLSSGTDRAVLLARKPPAA"
     gene            complement(3783..4967)
                     /locus_tag="VIF63_07110"
     CDS             complement(3783..4967)
                     /locus_tag="VIF63_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEY8239188.1"
                     /translation="MNKRPAAPRNGASSPNGPSSRNGANDTFGHQWGWDAEFEVFDDF
                     DLPTYVGPTTFSNLPWVTDPAELERRRVDVAVVGAPFDDMVTHRPGTRFGPRAIREAQ
                     YTSGNLNSLQLDVRPFEELTVVDAGDANVVPAMHERGHAMIYRKVREVAATGAIPIIL
                     GGDHSITWPSASAIADVRKPGSIGIVHFDAHADTAGESWGQLASHGTPMRRLIESGAV
                     LGRNFVQVGLRGYWPPTDVLEWMGEQGLRWHLMTEIEERGAEAVIDDAIAEALDGPDA
                     IYLSIDIDVVDPGSAPGTGTPEPGGMLPRELLRAIRRIVGTVDLAAMDIVEVSPPYDW
                     AESTAQLANRCALEAISALAVRQRDGHAVRFQPGAGKRGAGPRRAARAGTTARARTAA
                     TR"
     gene            complement(5081..5467)
                     /locus_tag="VIF63_07115"
     CDS             complement(5081..5467)
                     /locus_tag="VIF63_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239189.1"
                     /translation="MDSRDRRRTLIVFGLAGVAWAVVVAVAFLVDPLESQTAGYAGAG
                     ALGSAAALTAAPLFWLVGFARQRRIAYRGDWIRALRRGAWVGALVAVFVVMRLNGIFQ
                     PQIGLFLIALAIVAEVTLTAGSTSRG"
     gene            complement(5479..6309)
                     /locus_tag="VIF63_07120"
     CDS             complement(5479..6309)
                     /locus_tag="VIF63_07120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8239190.1"
                     /translation="MTGDHLRVAAITLDFGNTLVRVDRPGLRAVLEETADVLGRRGIV
                     HDRPGFLTAWAEERERQFREEVPEFREVDVAQRAVRVLARIRGMAPPPADARWDDLTA
                     AARVAPEEVDAVVDAYSAAFVSRMQPVADALATLEQLASRGFVLGILSNWPLALTVDR
                     FAEAQGWTRHLRAIVVSQRVGTIKPHPVIFRAAERALGLAPTDAARILHVGDDWAADV
                     VGASNAGWHTAYLRDRQADSPLPASKPGDGGMDDADVTADLEIDELSDLDGLVEVASA
                     "
     gene            complement(6381..9587)
                     /locus_tag="VIF63_07125"
     CDS             complement(6381..9587)
                     /locus_tag="VIF63_07125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8239191.1"
                     /translation="MIRTRNRRRIRPLEQIPVWHGFEPSAPAADPSIDGEAPAQPTGV
                     ESLLAGLNTEQRRAVTHGEGPLLVVAGAGTGKTQVITRRIAWLIATRRARPSEILALT
                     FTDKAAEEMQLRVDQLVPYGYADTAISTFHAFGDRLIREFAFELGLAPDVGVLTRPEV
                     VVFMRERLFDFGLEEYRPLGDPTRFLGALASLFARCRDEDVSPEAYAAHAAGLVARAA
                     ELAAAAAGADATDADRDAAVAAAELGRRQTELAHAYDRYVTLLREHGAIDFGDQVSLA
                     LNLVRTSAAARESLQARYRYILVDEFQDTNRAQSELVAMLAERHRNVTVVGDDDQSIY
                     RFRGAAISNILEFRERYRGARTVVLRRNYRSLAPILDGAHRLIRFNDPDRLEVKVGIS
                     KRLIAERVDHAAAPVRHHAFGTAGEEADWIAAEIRRRVDAGADVRDHAVLVRANADAD
                     PVLRSLNTAGLPWRFSGTSGLYARPEVRLLLALLRAVADPASSVDVYAIAASERYAIP
                     PGDLAEIAGSARRRHRTLFEILEELEDEPGVLRISPKGRLALAKLTVELRRYRDLAQR
                     RPAGEVLYAFLRDTGWLAELAGATSVAAEEQLANIGRFFDIIRTQSALLADDRAVFLG
                     RHLATLIEAGDDPPTADPDPDVDAVHVMTVHKAKGLEFPVVFLPGLVADRFPARSRRE
                     QLALPVELVDEVLPEGDGHLQEERRLFYVAMTRARDELILSHSAETSGGRARRVSPFV
                     VEALDLPSAGLRTDPFPSAVHATGPLARIAAAEPRSEPPEADRLPIEGPLMLSHSAID
                     DYLTCPLRYKYAQVVRVPTTPHHSMIYGSALHKAVQEFHRSQSRGKPLTETELLAVFD
                     ASWSNEGFVSREHETARLEAGREALRRFRESQLQPGSVVPAWVEREFAFALGGDRVRG
                     RFDRVDIVPVAARSSEVTGEVAEGPVPSVLRADVIEPMLELLGPEEVTITDYKSSDVR
                     DPQIAKQRARDSLQLQIYAMAYEAMTGRLPDAVQLHFLDSGLVGRAEVEPARLEKARA
                     KIATAAAGIRARDYTPKPGALACTYCPFRDICPSSVAR"
     gene            complement(9682..10146)
                     /locus_tag="VIF63_07130"
     CDS             complement(9682..10146)
                     /locus_tag="VIF63_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY8239192.1"
                     /translation="MLIVVFALVLAVVGLIRRTRKLGRRFESITRGADDQSLEAVLGS
                     HLERVRGVVRDVELVGARTAAIEKDLLGSFGRVGLVRFNPFEDTGGNQSFALALLDGR
                     GDGFIVSSLHARTGTRLYAKAVSGGTSETALSDEESTALQQALAKPVPGGTA"
     gene            10291..10764
                     /locus_tag="VIF63_07135"
     CDS             10291..10764
                     /locus_tag="VIF63_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239193.1"
                     /translation="MAKRTRYPNRSNAKPAAKKAAGRPAPAAGLTRAPTRPDTIDAHF
                     HDGHVHAEDDLPLRSSAGLTEAEERRAAELEAEATARERAAIAESLRRRARGQAAADG
                     LHDAGDINAPLSVRAAHEYAYVSRDVKRIALTASLMIAILAVLHILVNVLGVISL"
     gene            10765..12408
                     /locus_tag="VIF63_07140"
     CDS             10765..12408
                     /locus_tag="VIF63_07140"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8239194.1"
                     /translation="MTPADLVLVGGSVMTMNPANPSATGVAVSAGRIAAVGDDREIAT
                     RIGPRTRRIDLRGRTLLPGFVDAHVHPILAGIELLRCGLHDLPESSDAYLAFIRNYAQ
                     ERPDLEWIVGSGWGMAAFRGGTPSRADLDAVIPDRPAFFENRDGHGAWVNSRALELAR
                     IDRATPDPADGRIERTADGEPQGTLHEGAMDLVARLVPVDTPDEVVEGLALAQAYLHR
                     LGITAWQDAWVDPAALDAYRQFAAQGRLTARVVAAQWWDRHDDDRQIEALIDGRSRSS
                     IGRLTAGTVKLMLDGVIENFTASVLEPYLDGHGHSTGNRGIDFIEPEALKGIVTKLDA
                     LGFQAHFHALGDRAVRQGLDAVEAARNANGMTDTRPHVAHLQLVDPEDWPRFEALDAG
                     ANIQPLWARNEAQMTDLTIPFLPPERAELQYPFASLQRAGARLVGGSDWMVSTPDVMQ
                     QIEVAVRRVDPDHREREPFLPDETIDLDTALRAFTVGGAWANHADETTGSIGVGKLAD
                     LVVLDRDLAHEPLDRIGDARVLLTVVEGNALFEDPALET"
BASE COUNT         1874 a         4452 c         4534 g         1738 t
ORIGIN      
        1 gctcctcgcc catgatcgtt tccatccagt gctcgcccgt cgaatcgccg cggagcgccc
       61 gcccgatcgc ccgcagcgtg tcccgcccga gtgacgaagc gttgccgctg aacaggctga
      121 gctggcgctc gagccgctcc gcgatgtacg gccggagcca cggatgtgcc tcgaactcga
      181 aggcgtgggt cgtctcgtgc agggcgatcc acgtgcggaa cggcgcgacc gggatgccca
      241 gcgagtgtgc ggtggcgcgg atgttctcct cgacgaagag gagtcggccg tcctcggcct
      301 ccgccgagag cattgcgagg tcgtactggc cgagcacgcg cgtgcccatg aatccgagca
      361 ggaagccgag ctgttggctc gtcacccatc gattcgccag cgccatcgtg gcgcgcgtca
      421 gtccgccgcc ctccggcacg acctggtcga gcaggtcctg ctccatgcgc gagatgagcg
      481 agcggaacgt gccgacgttc gcccggatcc agcccgcccg atcgacgacg ctcgcgcgct
      541 ccaccacgcc cggcagggac gtgccgagcg cctcgctgag tcggggcacg acgcgggcca
      601 tcgcctcgcg gtaggcgccg tcggcggcgc gcagctcgag tggcgagagg cggccgggcg
      661 cccgttctgc gcgccggatc gcgagccgct ccaccgcacc ccagtcgacg aggccgcgcc
      721 gagcgatgcg ctccgctcga cgcccgagca ccgtcgcggc cgcgccgatc gccgacccga
      781 ggatgaagcc cgcctgccag cgccggtccg cgcgccagcc ctggggccgc cggccggggg
      841 cgcgggagct gcgattctcg ctcacgtggc gctgcccatc gccgccaggt cgatcgcggc
      901 cacatccggg aatgcctcgc cgaacatccg ctccgcgatc gagcggaagg cgtcgacatc
      961 gccggtggtg agctggaggt gggtcgcccc gccgggcgcc gcgtcgagcc cgttgatcga
     1021 gaggagctcg gcgagcgcgg aggcggtcgc cgccgcggaa tcgacgatcg cgatgccatc
     1081 gcccgccacc gcggcgatgg ccgcgcgcag gagcgggtag tgcgtgcagc cgaggaggag
     1141 ggtgtccacc ttcgcggacg ccggcagcgg gaacacgaac tcgccggccg cgtcgcgctc
     1201 gccgaggagc ggcgcaaggg cctcgcggac gctcgcctcg acctcggcgc cggtcaggag
     1261 tccggcttcg accatcggca cgaacgtcgg cgtcgcgtgc tcgtacacct cgaccgcggg
     1321 gttctcatcc ttgatcgccg agaagtacgc gtgcgagcgg atcgtcgccg gcgtgccgat
     1381 gacgcccacg cgccggttac gcgacgcgag cgcggcggat gtcgcacccg gccggatcac
     1441 gcccaggatc ggcaggtcgt agcggcgccg aaggtcggcg agggcaattg ccgtcgaggt
     1501 gttgcaggcg acgacgaccg ccttgacgtc gcgcgcgacc agcgcgtcga tcgactcgat
     1561 cgagaaggtg cggacctcgg cgtcggggcg cacgccgtac ggggcgcgcg cgttgtcgcc
     1621 gaagtagatg gtcgattcgt cgggcgtccg gcggaggact tcacggagga cggtcaggcc
     1681 gccgacgccg gagtcgaaga cgccgatggg tcgcggatca gtcacccggg aatgatggcc
     1741 ctcaccggcg gagtgcgctg gctaccgcgc cgcggcgagc tgccggtcgc tgcgcgcgcc
     1801 cgcggccgcc ttgccgaaca tctggatcgc aacctcgatg aggtccttgc tgacgcccgc
     1861 ggccggctcg gccgtgacga acggtgcgcc ctggttgctc gcgggaccga cgacattgcc
     1921 ttcggagcgg atcttgaact cgggctcgcg accgagggca ttgcgcaggt cgtttgggtc
     1981 gatgcccgcc gaggcgtcgg cgcgattcac gaggtacttc accttgctcg gcgggtagcc
     2041 gatcttctgg aacgtctcgg ccatcgcgat cgtgttgcgg atcgtcgtcg cgtcgtaggt
     2101 gacgatctcg aggatggtgt ccgcctggtc gaggagggcg agggtcagct cgtcgagccc
     2161 gacgccggta tcgacgatca cgatgtcgta gaggcggcgc atcagcgaga gcgtcttctc
     2221 gaggtcgcgg atgaggacca tctcggccat ctcgatccgt ggcggcgcga gcaggacgtc
     2281 gacatcggcc gggccgcgcc agagcacgtc cgcgagatcg agctcggaga tgcggtcggt
     2341 cggcaggtcg agcatcgacg gcgcctcgcg cggcgcccgc agcagcccgc gcaggtcacc
     2401 gaactggatg ctgccgtcga cgagcgccac cttcaggccc aggcccgacg cagcgacgcc
     2461 gaggttgaac gcgattgtcg acttcccgac gccgcccttg ggcgcgaaga ccacgacgag
     2521 gcggctcggg ccgcgctccg ttgccgccgt agcggtcttg tgcacgccgg cgatgcgggt
     2581 gatgaggtcg tagccgttga acggcatcga caggacctcg tcgatcccct tctcgggttc
     2641 gacggtgacc gggttctggg gcacggtgag gacgatcacc gggacgccgg tcttcgagtc
     2701 gtggagctgc ttgaccagct gcatcccgcg caggcgaccc tgcagcaacg cgtcgacgac
     2761 gatcacgtcc gggcggtgtt cgctgatccg catcgggacc atcgtcccgt ccgacacgat
     2821 gtcgacgaga cgcacctgac tctgggcgtt cagcagccca cggacgtact gtgcgacctg
     2881 gggcacgtcc tcgacgagga gcaggcgaat ctgatcaccg gaggccatcg ccggaacggt
     2941 accacgagga accggccgtt ccggaccctg ctaggcggca gggggtttgc gagccaaaag
     3001 taccgcccga tcggtgcccg acgacagcgg cgagaggtcg tgatcgcctg ccagctgctc
     3061 gacctggagg cccgcgtcgg tcgcgtagtc gaccagctcg tcggcgctga tgagccgcag
     3121 ggcgtcggcc cgcgtccagc gaatcggggc ggttcccggg gcgccctcct cgaacaccgt
     3181 ggtgagggtc acgattcgga gcgtgggatc gaaccaagcg gcgctggtct tggtgacttc
     3241 gaacccggtc tcgggatcgg tgcgcagcca gtcgagcgag agccggccgt cgaacgccgt
     3301 caggtccgat gggaccggca gccaggtgtc gacgacggcg aggccgcccg gcgcgaggag
     3361 ccgggccatc gacgcgaaga cggctcgctg gcgcgcgggc gtcgacatga tcaggatcga
     3421 gttgagggcg aggatcgcga gctcgtacgg gccgcgtgcg agcacctcgg ggtgcgtgcc
     3481 ggcggtcgtc atgtccgcct cgatgagctc gaggcgatcg gcgacaccct tgggtgcctg
     3541 cgcgatgcgg gcgcgggcgc gggcgagcat cgcgggttcg atgtcgacgc cgacgacgcg
     3601 atgaccgtcg gccgcgagcg ggacggcgat cctgcccgag cccacggcga actcgacgat
     3661 cggcccgccc gtgcgccggg cgagggcctg gaagagctcg acgtcgcccg cgtcgacgct
     3721 caggtcgagg tcgtacagcc gcgcgatcgc ggccgcgcga gcagtctcgt cggcgacgcg
     3781 ggtcacctgg tggctgcggt tcgagctcgc gcggttgttc cggctcgcgc ggcgcgccgg
     3841 ggcccggcgc cacgcttgcc cgcgcccggc tggaaccgga ccgcgtgccc gtcgcgctgc
     3901 cggacggcaa gggccgagat cgcctcgagg gcgcaacggt tggcgagctg ggcggtcgac
     3961 tcggcccagt catacggggg cgagacctcg acgatatcca tggccgcgag atcgaccgta
     4021 ccgacgatcc ggcggatcgc ccgaaggagc tcgcgcggca gcatcccgcc cggctcgggc
     4081 gttccggtgc ccggcgccga gccgggatcg acgacgtcga tgtcgatgga gaggtagatc
     4141 gcgtcaggcc cgtcgagcgc ctcggcgatc gcgtcgtcga tgacggcctc ggcgccgcgc
     4201 tcctcgatct cggtcatgag gtgccagcgg aggccctgct cgcccatcca ctcgagcacg
     4261 tcggtcggcg gccagtagcc gcgcagcccg acctggacga agttgcgacc gagaaccgcg
     4321 cccgactcga tcaggcgtcg catcggcgtg ccgtggctcg cgagctggcc ccaggattcg
     4381 cccgcggtgt cggcatgcgc gtcgaagtgg acgatgccga tgctgccggg cttgcgcacg
     4441 tcggcgatcg cgctggcact cggccaagtg atcgagtggt cgccgccgag gatgatcggg
     4501 atcgcaccgg tcgccgcgac ctcgcggacc ttgcgataga tcatcgcgtg gccgcgctcg
     4561 tgcatcgccg ggacgacgtt ggcgtcgccc gcatcgacga cggtcagctc ctcgaacggg
     4621 cggacgtcga gctggagcga gttcaggttg cccgacgtgt actgggcctc gcgaatggcg
     4681 cgcgggccga acctcgtgcc gggccggtgc gtgaccatgt cgtcgaacgg cgcgccgacg
     4741 acagcgacgt cgacccgacg tcgctcgagc tccgctgggt cggtgaccca gggcaggttc
     4801 gagaacgtgg tcggcccgac ataggtcggc aggtcgaagt cgtcgaagac ctcgaactcg
     4861 gcatcccagc cccactggtg gccgaaggtg tcgttcgccc cgttgcggga cgatgggcca
     4921 ttcggggacg aggcgccatt gcggggcgcg gcggggcgct tgttcacgtc gatccttcgg
     4981 atcggcccgc gaaatgagcg gcagccgatc gcgtccgacg ccgccggttc ggaatccgcc
     5041 gtccagcggg gcgccagccg gcatcctacc accgcccgaa tcagcctcgg ctggtacttc
     5101 ctgccgtgag cgtcacttcc gccacgatgg ccagggcaat caggaacagc ccgatctgcg
     5161 gctggaagat cccgttgagg cgcatcacca cgaagacggc caccagcgcg ccgacccagg
     5221 cgccgcgacg aagcgcccga atccaatcgc cgcgataggc gatccgccgc tggcgcgcga
     5281 agccgacgag ccagaacagc ggtgccgcgg tcagggccgc cgccgaaccc agcgctccgg
     5341 cgccggcgta gcccgcggtc tggctctcga gcgggtcgac gaggaacgcg accgcgacga
     5401 cgaccgccca cgcgacgccc gcgaggccga acacgatgag ggtgcgtcgc cggtctcggg
     5461 aatccatcgg gcgggagtct aggccgacgc gacctcgacc agcccatcga gatcggagag
     5521 ctcgtcgatc tcgagatcgg ccgtcacgtc ggcgtcgtcc atgccgccgt ctccgggctt
     5581 cgacgccggg agcggcgagt ccgcctggcg gtcgcgcagg taggccgtgt gccagcccgc
     5641 gttgctcgct ccgaccacat cggccgccca gtcgtcgccg acgtgcagga tgcgcgccgc
     5701 atcggtcggt gcgaggccga gcgccctctc ggcagcgcgg aagatcacgg gatggggctt
     5761 gatcgtgccg acccgctgcg acaccacgat cgcccgcaga tgccgggtcc agccctgcgc
     5821 ctcggcgaag cggtcgaccg tcagcgcgag cggccagttc gacaggattc cgaggacgaa
     5881 cccgcgactc gcgagctgct ccagggtcgc gagcgcgtcg gcgacgggct gcatccggct
     5941 cacgaaggcg gcgctgtacg cgtcgacgac cgcatcgacc tcctccggcg cgacccgcgc
     6001 ggccgcggtg aggtcatccc agcgcgcgtc cgccggcggt ggcgccatgc ctcggatgcg
     6061 ggcgagcacc cgcaccgcgc gctgggcgac gtcgacctct cggaactcgg gcacctcttc
     6121 gcggaactgg cgctcccgct cctccgccca cgcggtcagg aacccgggcc ggtcgtgcac
     6181 gatcccccgc cgcccgagga catcagcggt ctcttcgagg actgcgcgga gcccgggccg
     6241 gtccacgcgc acgagggtgt tgccgaagtc gagcgtgatc gcggcgacgc gcaggtggtc
     6301 gccggtcaac gctggtcgcc ggtcaacgct ggtcgccggt caacgcgggc ggcgcaccgc
     6361 tcggcgcgcg aagggcacgc tcatcgggcc acgctcgagg ggcagatgtc gcggaacggg
     6421 cagtacgtgc aggcgagcgc gcccggcttc ggcgtgtagt cacgcgcccg aatccccgcc
     6481 gcggcggtcg cgatcttcgc gcgcgccttc tccagccgcg ccggctcgac ctctgctcgg
     6541 ccgacgaggc ccgagtccag gaagtggagc tggacggcat cgggcagccg gcccgtcatc
     6601 gcctcgtagg ccatcgcgta gatctgcagc tgcagcgaat cgcgcgcccg ctgcttggcg
     6661 atctgtgggt cgcggacgtc gctcgacttg tagtcggtga tcgtgacctc ctcggggccc
     6721 aggagctcga gcatcggctc gatcacgtcg gcgcgaagca cggacgggac cggtccttcc
     6781 gcgacctcgc cggtcacctc gctcgatcgc gcggcgacgg gcacgatgtc gacccggtcg
     6841 aagcggccgc gcacccgatc gccacccagt gcgaacgcga actcgcgctc gacccacgcc
     6901 ggcaccaccg acccgggctg gagctgcgac tcgcggaacc ggcgcagcgc ctcgcggccg
     6961 gcctcgagtc gcgccgtctc gtgctcgcgc gagacgaagc cctcgttcga ccacgacgcg
     7021 tcgaagaccg cgagcagctc ggtctccgtg agcggcttgc cccgcgactg cgagcggtgg
     7081 aactcctgca ccgccttgtg gagcgcggag ccgtagatca tcgagtggtg cggcgtcgtc
     7141 gggacgcgga cgacctgggc gtacttgtac cggagcgggc acgtcagata gtcgtcgatc
     7201 gcggagtggc tgagcatcag cgggccctcg atcggcagcc ggtccgcttc gggcggctcg
     7261 gatcgaggct cggccgccgc gatcctggcc agcggaccgg tcgcgtgcac cgccgacggg
     7321 aacgggtcgg tccggaggcc cgccgacggc aggtccagcg cctcgacgac gaagggcgac
     7381 acgcggcggg cgcgcccgcc cgaggtctcg gcggaatggc tcaggatcag ctcgtcgcgc
     7441 gcccgggtca tcgccacgta gaacagccgg cgctcctcct ggaggtgtcc atcaccctcg
     7501 ggcagtacct cgtcgacgag ctcgaccggc aacgcgagct gctcgcgccg cgagcgggcc
     7561 gggaaccgat ccgccacgag gcccggcagg aagacaaccg ggaactcgag gcccttcgcc
     7621 ttgtgcacgg tcatcacgtg gacggcgtcg acgtccggat cgggatcggc cgtcggcggg
     7681 tcgtcgcccg cctcgatgag cgtcgcgaga tggcgaccga ggaagacggc ccgatcgtcg
     7741 gcgaggagcg cggactgcgt ccggatgatg tcgaagaacc ggccgatgtt cgcgagctgc
     7801 tcctcggcgg cgacggacgt cgcgccggcg agctcggcaa gccagcccgt gtcgcgcagg
     7861 aacgcgtaga gcacctcgcc ggcaggccgg cgctgggcaa ggtcgcggta gcggcgcagc
     7921 tccacggtga gcttcgccag cgcaagccgg cccttcggcg agattcggag gacgcccggc
     7981 tcgtcctcga gctcctcgag gatctcgaac agggttcggt gccggcgccg cgcggaccct
     8041 gcgatctcgg caaggtcgcc cggcggaatt gcgtagcgct ccgacgcggc gatggcgtag
     8101 acgtcgacgc tcgacgccgg gtcggcaacc gcgcggagca gcgccaacag gagccgcacc
     8161 tcggggcggg catacagccc ggacgtgccg gagaaccgcc agggcagccc agccgtgttc
     8221 agcgatcgga gcacgggatc ggcatcggcg ttggcgcgga cgagcacggc gtgatcgcga
     8281 acgtcggcgc cggcatcgac ccgacgccgg atctccgcgg cgatccagtc ggcctcctcg
     8341 ccggccgtgc cgaacgcgtg gtgccggacc ggggcagcgg cgtgatcgac gcgctcggcg
     8401 atgagccgct tcgagatccc gaccttcact tcgagccggt ccgggtcgtt gaaccggatg
     8461 agccggtggg cgccgtcgag gatcggcgcg agcgagcggt aattccggcg caggacgacg
     8521 gtccgcgcgc cgcggtaccg ctcgcggaac tcgaggatgt tgctgatcgc ggcgccgcgg
     8581 aaccggtaga tcgactggtc gtcgtcgccg acgacggtca cgttgcggtg gcgctcggcg
     8641 agcatggcga cgagctccga ctgggctcgg ttcgtgtcct ggaactcgtc gacgaggatg
     8701 tagcggtagc gcgcctggag cgactcgcgc gcggccgcag acgtgcgcac gaggttgagc
     8761 gcgagcgaga cctggtcccc gaaatcgatg gcgccgtgct cgcgcagcag cgtgacgtag
     8821 cggtcgtacg cgtgtgccag ctccgtctgg cgtcgcccca gctcggcagc cgcgacggcc
     8881 gcatcgcgat cggcatctgt ggcgtcggcg cccgcagcgg cagccgcgag ctcggccgcg
     8941 cgcgcaacca gtcccgcggc gtgcgccgcg tacgcctcgg gggagacgtc ctcgtcgcga
     9001 catcgcgcga acagcgacgc caacgctccc aggaaccgcg tggggtcgcc gagcgggcgg
     9061 tactcctcga gcccgaagtc gaacagccgc tcgcgcatga acacgacgac ctcgggccgg
     9121 gtgagcacgc caacgtcggg cgccaacccg agctcgaacg cgaactcgcg gatcaggcga
     9181 tcgccgaatg catggaacgt cgagatcgcc gtatcggcgt agccgtacgg cacgagctgg
     9241 tccacccgga gctgcatctc ctcggcagcc ttgtccgtga aggtgagggc gaggatctcc
     9301 gagggtcgcg cgcgacgggt cgcgatcaac cacgcgattc ggcgcgtgat gacctgggtc
     9361 ttgcctgtcc ccgcaccggc cacgaccagg agcggcccct cgccatgcgt gaccgctcgg
     9421 cgttgctccg tgttcagccc ggcgaggagc gactcgacgc ccgtcggctg ggcgggcgcc
     9481 tcgccatcga tcgacgggtc ggccgcgggc gcgctcggtt cgaacccatg ccagaccgga
     9541 atctgctcca gggggcggat ccggcggcga ttccgcgtcc ggatcatcgg gtccggaacc
     9601 ggtggtattg ggggcatgcg accaccttcg ccgacggtcg tgacagctct gctgacactg
     9661 ccgccgttcc ccccaccatc ctcaggccgt gccgcctggt accggcttcg ccagcgcctg
     9721 ctggagcgcg gtcgactcct cgtcggacag ggcggtctcc gacgtcccgc cgctcaccgc
     9781 cttcgcgtac aggcgcgtgc ccgtccgcgc gtggaggctg ctcacgatga agccgtcgcc
     9841 gcggccgtcg agcagcgcga gcgcgaagct ctggttgcca ccggtgtcct cgaacgggtt
     9901 gaaccggacg agcccgaccc gtccgaacga gcccagcagg tccttctcga tcgccgccgt
     9961 tcgcgcgcca acgagctcca cgtcccggac cacgccgcgc acgcgctcaa gatggctgcc
    10021 gagcacggcc tcgagcgact ggtcgtcggc gccgcgggtg atgctctcga accgccgccc
    10081 gagcttgcgc gtccgccgga tgagcccgac gacggcgagc acgagggcga agacgacgat
    10141 gagcaacccg gcgacgatcg ggccgatgtt ggcctggacg agcgcgtcga gatcgatcgg
    10201 acgtcctcct cggtacgcct gggggcgggt gcgtcgagtc tagccgccgc cgattgcccg
    10261 cggctcaccc tttgctatcc ttccgcggcc atggcgaaac gcacccgcta cccgaatcgc
    10321 tcgaacgcga agcctgccgc gaagaaggcg gccggacgcc ccgcccccgc cgcgggcctc
    10381 acgcgagcac cgacgcggcc cgacacgatc gatgcgcact tccacgacgg ccacgtccac
    10441 gccgaggacg acctgccgct tcgcagctcg gccgggctga ccgaggccga ggagcggcgc
    10501 gccgccgagc tcgaagccga ggcgaccgcc cgcgagcgcg ccgcgatcgc cgaatcgctc
    10561 cgacgcaggg cgcgtggcca ggccgccgcc gatgggctcc acgacgcagg cgacatcaac
    10621 gccccgctct cggttcgcgc cgcgcacgaa tacgcgtacg tctcgcgcga cgtgaagcgc
    10681 atcgcgctca ccgcgagcct gatgatcgcg atcctcgccg tcctgcacat cctcgtgaac
    10741 gtgctcgggg tgatctcgct ctagatgacg ccagccgacc tcgtgctggt cggcggttcg
    10801 gtcatgacca tgaaccccgc gaatccttcg gcgaccggcg tcgccgtctc ggccggccgg
    10861 atcgcggccg tcggtgacga ccgggagatc gcgactcgca tcggcccgcg gacgcgacgg
    10921 atcgaccttc gaggtcggac gctgcttccc ggcttcgtcg acgcccacgt ccatccgatc
    10981 ctcgccggca tcgagctcct ccggtgcggc ctgcacgacc tgccggagtc gagcgacgcg
    11041 tacctcgcgt tcatccggaa ttacgcccag gagcgacccg atctcgagtg gatcgtcggg
    11101 tccgggtggg gcatggcggc attccgaggc ggtacgccat cccgcgccga cctcgacgcg
    11161 gtgatccccg atcgaccggc ctttttcgag aaccgcgacg gccacggcgc gtgggtgaac
    11221 agccgggcgc tcgagctcgc ccgcatcgat cgagcgacgc ccgacccagc ggacggtcgc
    11281 atcgagcgca ccgcggacgg cgagccgcag ggcaccctcc acgaaggcgc gatggacctc
    11341 gtcgccagac tcgtcccggt cgacacgccg gacgaggtgg tcgagggcct cgccctcgcc
    11401 caggcctacc tccatcggct cggcatcacc gcctggcagg acgcgtgggt cgatcccgcc
    11461 gccctcgatg cctatcggca gttcgccgcc cagggtcggc tgaccgcgcg ggtggtggcc
    11521 gcccagtggt gggaccggca cgacgacgat cgacagatcg aggcgctgat cgacggccgg
    11581 tcacgctcat ccatcggtcg gctcaccgcc ggcacggtga agctgatgct cgacggggtg
    11641 atcgagaact tcacggcgtc ggtgctcgag ccgtacctgg acggtcacgg tcattcgacg
    11701 ggcaaccggg gcatcgactt catcgagccc gaggcgctca aggggatcgt gacgaagctc
    11761 gacgcgctcg gcttccaggc gcacttccat gcgctcggcg atcgagccgt ccgccagggc
    11821 ctcgacgccg tcgaggcggc gcggaacgca aatggcatga cggacacgag gccgcatgtt
    11881 gcccatctcc aactcgtcga cccggaagac tggccacgct tcgaggccct ggacgcgggc
    11941 gcgaacatcc agccactttg ggcacgcaat gaagcccaga tgacggacct cacgatcccg
    12001 ttcctgccgc ccgagcgggc cgagcttcag tacccgttcg cgagcctgca gcgcgccggc
    12061 gcccgactgg tcggtggcag cgactggatg gtcagcaccc cagatgtcat gcaacagatc
    12121 gaggtcgccg ttcggcgcgt cgacccggat catcgggagc gagaaccgtt cctgccggac
    12181 gagacgatcg atctcgacac ggccctacgg gcattcaccg tgggcggggc gtgggccaac
    12241 cacgcggacg agaccacggg ctcgatcggc gtcggcaagc tcgccgacct cgtcgtcctc
    12301 gatcgggacc tcgcgcacga accgcttgac cggatcggcg acgcccgggt gctgctgacg
    12361 gtcgtcgagg gcaacgcgct gttcgaggat cctgccctcg aaacctagtc cacgcatacg
    12421 atggccaggg catgctggac gagaagcgcc aagcaggagg catttcagat gagcgattac
    12481 gccgtcgtga acccggcgac cggggagacc gtggcgcgct acgacacctt caacgatgcc
    12541 caggtcgagg gcgcgctcgc caaggcccag gcggcgttcc tgacctggcg gaaggtcc
//
LOCUS       DATBSW010000254         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_94845, whole genome shotgun sequence.
ACCESSION   DATBSW010000254 DATBSW010000000
VERSION     DATBSW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94845"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VIF63_07145"
     CDS             <1..1496
                     /locus_tag="VIF63_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239195.1"
                     /translation="LFTLRAYLSADVISILGDEPSYFFNKREDEGHISAELADPASHV
                     AHLGEILDEVDAGVTDAALKKRLFARFYRIEVLARLAGPQFLGAPAEYQQELFTALRT
                     IARTRFAAVIQEGLGAFARIRSRLLLEGALPELIALGERAAGVTLDARVLRAAWTGGR
                     LGIDFRVALSRGADARPLTVIERDGRLLLDPDVADDLVGPVDVTDEMASIRAQVSVVD
                     RATSLEWIVPGATRLALRPGREPDLGARRPALVGFVALDPQRVGPGEQPLDDGAWDVV
                     IRWLGLGMQATGGMRLSRKARSGAGPPIPPALVGTPVRWLVPRADASGALRIGIGGPD
                     RVPARMDDAARRVLRQGRGVAIELPVATERTGSLAAGTLRLTGPAGAFDLPASIDGAL
                     GLLVVVVEDVTAGGAIPRGRYEMTAHIGGEAAPGLPVGSVVVRDDGRFVVLGVEPVSQ
                     VARLRSVATWTARSAGSAVRGRAVATYRRLPSWAKDVVRTGRGRLRA"
     gene            1528..2415
                     /locus_tag="VIF63_07150"
     CDS             1528..2415
                     /locus_tag="VIF63_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239196.1"
                     /translation="MSQGESAASIRHAAGNLSPILLLRQGITDILSRQRLVRYLVQAE
                     MKKRGSDTVLGNLWWILDPLLQMVVYVVFITIIARNPAPDYPLFIFAAILPWKWFSAV
                     ISDATTAVVRHDKLIRQIAFPKLVLPMATTTAGVVGFAWGLVPLFALLLLHPDRLSLM
                     VIWIPIIAAVQYVFTLAAAILISAANVFFRDLGNAVGHFLRLWWFLSPGLYSLAALEG
                     LEVLKDHPTIKFLAGFNPFAVLFEAYRAVVYGAPVVGGKPYPPDLVALGFLLLGSLVF
                     LGLCIVFFKRVEPDFAKVL"
     gene            2412..3260
                     /locus_tag="VIF63_07155"
     CDS             2412..3260
                     /locus_tag="VIF63_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239197.1"
                     /translation="MSLPPAARRPPSRSSSRYAISVNDLGVQYNLRFSRKTTLRQSVA
                     NLVARKPPERFWALRHVDVNLSRGESLAVIGPNGAGKSTFLQVLAGIMRPSEGSVDVR
                     GQVSGLLGLGVGFDVELSGIDNVLLGGAFLGLDGPRIRALLPSIVEFADLGQFIDAPL
                     KTYSSGMRARLGFAIATAVDPDILLLDEVLATGDANFRTKSKARVIELVRAAKGVVLV
                     THDMDWVREYCNRALLLEKGMVILEGAPDEVTALHLERQKEAAERRKAEAEAKGLDPA
                     IARKKG"
     gene            complement(3530..4060)
                     /locus_tag="VIF63_07160"
     CDS             complement(3530..4060)
                     /locus_tag="VIF63_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239198.1"
                     /translation="MAENGATPIAPKRPRAPRKPKVTIVVPEASSLEPVAPAVVEDGT
                     SLAAEAVEIHQGAVGRVEAGTVTVSQGAIGAVRADHVSVERGAVAAAIGEHVEVSRGY
                     ARSILARQVQMDRAAARVVIAADVRAEQTAVMFLIARRVDGNLRVLLDWRGALAFGAV
                     AGVVFAVLSRIRNKGR"
     gene            4152..>4555
                     /locus_tag="VIF63_07165"
     CDS             4152..>4555
                     /locus_tag="VIF63_07165"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY8239199.1"
                     /translation="MAHSTAPGVFTMRRGLIVLIGVAIAALGLLVASQIGTLLPGGAA
                     AATSSPGPLATVGQSPVAVTPSPTPAPPTAPPPTPIPTPPTVPGPLTGLPVSPEAALH
                     HPIAVMIDDDSHARPQGGFNAASIVWHAPAEGG"
BASE COUNT          595 a         1661 c         1585 g          714 t
ORIGIN      
        1 agctgttcac gctccgcgcg tacctgtcgg cggacgtgat ctcgatcctg ggcgacgagc
       61 cgagctactt cttcaacaag cgcgaggacg agggccatat cagcgccgag ctcgccgacc
      121 cggcgtcgca cgttgcccac ctcggcgaga tcctcgacga ggtcgacgcg ggggtgacgg
      181 atgccgcgct caagaagcgg ctgttcgccc gcttctaccg gatcgaggtc ctggcccgcc
      241 tcgccggccc gcaattcctg ggcgcgccgg ccgagtacca gcaagagctc ttcaccgccc
      301 tcaggacgat cgcccggaca cggtttgcgg cggtgatcca ggagggcctc ggtgcctttg
      361 cccggatcag gtcgcggctg ctgctcgagg gcgctctgcc ggagctcatc gccctcggtg
      421 agcgggccgc cggggtcacc cttgacgccc gggtgctgcg cgccgcctgg acgggtggcc
      481 ggctgggcat cgactttcgg gtcgcgctgt cgcgtggggc cgatgcccgg cccttgaccg
      541 tgatcgagcg cgatggccgg ctgctcctcg acccggacgt tgccgacgac ctcgtggggc
      601 cggtcgacgt caccgacgag atggcgtcga tccgcgccca ggtgtcggtc gtcgaccggg
      661 caacgtcact ggagtggatc gtgcccggtg ccacgcggct cgcgttgcga ccgggccgcg
      721 agccggacct aggtgcgcgg cggccggcgc tggtgggctt cgtggcgctc gatccgcaac
      781 gggtcgggcc gggcgagcag ccgctcgacg acggcgcctg ggatgtcgta atccgctggc
      841 tcggcctcgg gatgcaggcg acgggcggca tgcgcctgtc gcgcaaggcc cggtccggcg
      901 ccggcccgcc gatcccgccc gcgctcgtcg gcacgcccgt tcgctggctg gtcccgcgag
      961 cggacgcgag cggggcgctc cggatcggca tcggcggccc cgatcgcgtc ccggcgcgca
     1021 tggacgacgc cgcccgccgg gtcctgcgcc aggggagggg cgtggcgatc gagttgccgg
     1081 tcgccacgga gcgcaccggc tcgctcgcgg ccggcacgct gcgcctcacc ggccccgccg
     1141 gcgccttcga cctccccgcc agcatcgacg gcgcgctggg gctgctcgtc gtcgtggtcg
     1201 aggacgtcac cgccggtggc gcgattccgc gcgggcgcta cgagatgacg gcgcacatcg
     1261 gcggcgaagc agcgcccggg ctgccagtgg gcagcgtcgt cgtccgcgac gacggccggt
     1321 tcgtcgtgct cggggtcgag ccggtgtcgc aggtggcgcg cctgcgctcg gtcgcgacgt
     1381 ggacggcacg gtctgcaggc agtgcggtgc gaggccgggc ggtcgcgacg taccggcgcc
     1441 tgccgagctg ggcgaaggac gtcgtgcgaa ccggccgggg gcggctccgt gcctgagcgg
     1501 aacggggccc gtgctaccct cgcgccgatg tcccagggcg aatctgccgc ctcgatccgg
     1561 cacgccgcgg gcaacctctc accgatcctg ctgctgcgcc agggcatcac cgacatcctg
     1621 tcgcgccagc ggctcgtgcg ctatctcgtc caggccgaga tgaagaagcg cggctcggac
     1681 acggtgctcg ggaacctgtg gtggatcctc gacccgctgc tccagatggt cgtgtacgtc
     1741 gtcttcatca cgatcatcgc ccgcaacccc gcgccggatt acccgctctt catcttcgcc
     1801 gcgatcctgc cgtggaagtg gttctcggcg gtgatcagcg acgccacgac cgcggtcgtg
     1861 cgccacgaca agctgatccg ccagatcgcc tttcccaagc tcgtcctgcc gatggccacc
     1921 acgaccgccg gtgtcgtcgg cttcgcctgg ggcctcgtgc cactgtttgc gctgctcctc
     1981 ctgcatcccg accggctgag cctgatggtc atctggatcc cgatcatcgc ggcggtccag
     2041 tacgtgttca cgctggcggc ggccatcctc atctccgccg cgaacgtgtt cttccgggac
     2101 ctgggcaacg cagtcggcca cttcctgcgc ctgtggtggt tcctgtcgcc gggcctgtac
     2161 agcctcgccg ccctcgaggg gctggaggtg ctgaaggacc acccgaccat caagttcctc
     2221 gccgggttca acccgttcgc ggtcctgttc gaggcctatc gggccgtggt ctacggcgcg
     2281 ccggtcgtcg gcgggaagcc gtatccgccg gacctcgtcg ccctcggctt cctgctcctc
     2341 ggcagcctcg tcttcctcgg gctgtgcatc gtcttcttca agcgcgtcga acccgatttc
     2401 gcgaaggtcc tgtgagcctc ccgccagccg cccggcgacc gccgagccgc tcgtcgagcc
     2461 gctacgcgat ctcggtcaac gatctcggtg tccagtacaa cctgcggttc agccggaaga
     2521 cgacccttcg ccaatctgtc gcgaacctcg tggcacgcaa gccgcccgaa cggttctggg
     2581 cattgcgcca cgtcgacgtc aacctgagcc ggggcgaatc gctcgccgtc atcggtccga
     2641 acggcgcggg caagagcacg ttcctccagg tcctggccgg gatcatgcgg ccgagcgaag
     2701 gctcggtcga cgtgcgcggc caggtgtccg gcctgctcgg actgggcgtc ggcttcgacg
     2761 tcgagctatc cggaatcgac aacgtgctcc tcggcggcgc cttcctcggc ctcgacgggc
     2821 cacggatccg ggcgctgctg ccgtcgatcg tggagttcgc cgatctcggc cagttcatcg
     2881 acgccccgct gaagacctac tcctcgggca tgcgggcgcg gctcggcttc gcgatcgcga
     2941 cggcggttga cccggacatc ctgctgctcg acgaggtgct cgcgaccggc gatgcgaact
     3001 tccggacgaa gtcgaaggcg cgggtgatcg agctcgtgcg agccgcgaag ggcgtcgtcc
     3061 tcgtgaccca cgacatggac tgggtgcgtg agtactgcaa ccgggccctg ctgctggaga
     3121 agggcatggt catcctcgag ggcgcgccag acgaggtcac cgcgctccat ctcgagcgcc
     3181 agaaggaggc ggcggagcgc cgcaaggccg aggccgaggc aaagggcctc gatcccgcga
     3241 tcgcgcgaaa gaagggctag gtccgggggc aatcgcccgc gcgatgtgct cgtttgtgtc
     3301 aggtgttact ggggcgggcg ctggtcagag cgtgcgcatc gtttcctcgg ccgacgagcg
     3361 tggccgtgtc gcctgattcg cgcagatgcc ggtcctgcaa cccatctgac cgattcccgg
     3421 tgacgagtgc ccgggtgagt aacgactgac ccaatcggcg atcggtcgaa ggcaaacgta
     3481 agcgaacttg gacagggcac gatcctgcgc tcggtgaacc cgcagcggct cagcgcccct
     3541 tgttgcggat cctcgagagc accgcgaaga cgacaccggc gacggccccg aaggcgagtg
     3601 cgccacgcca gtcgagcagc acgcgcaggt tgccgtccac ccggcgggcg atgaggaaca
     3661 tcaccgcggt ctgctcggcc cgcacgtcgg cggcgatcac gacccgcgcc gcggcgcgat
     3721 ccatctggac ctgtcgggcg aggatcgatc gggcgtagcc ccggctgacc tcgacatgct
     3781 cgccgatcgc cgccgcgacg gccccgcgct ccaccgagac gtggtccgcc cggaccgcgc
     3841 cgatcgcacc ctgggacacg gtcacggtcc cggcttcgac ccggccgaca gcgccctggt
     3901 ggatctcgac ggcctcggcg gcaagtgatg tgccgtcctc gacgaccgcc ggcgccacgg
     3961 gctcgaggct cgacgcctcg ggcacgacga tcgtgacctt cggcttgcgc ggcgcccgcg
     4021 ggcgcttcgg cgcgatcgga gtggctccgt tctcggccat cgacctgtct cccatgtggt
     4081 ccgccagcct acgccctcgg cgtcacgcta tcctgcgcgg gcggtcggcg cccggcgccg
     4141 gatggaggga cgtggcgcac tcgacagctc ccggcgtgtt cacgatgcgg cgcggcttga
     4201 tcgtgctcat cggcgtcgcc atcgccgcgc tcggtctgct cgtcgcatcg cagatcggca
     4261 cgctcctgcc cggcggcgcg gccgcagcca cgagctcgcc cggtccgctc gccacggttg
     4321 gtcaatcgcc cgtcgccgtc acgccatcac cgacacccgc gccgcccacg gcacccccac
     4381 caacgccgat cccgaccccg ccgaccgtcc ccgggcccct gaccgggctg cccgtgagcc
     4441 ccgaggccgc gctccatcac ccgatcgccg tgatgatcga tgacgactcg cacgcccggc
     4501 cgcagggcgg gttcaacgcg gcgtcgatcg tctggcatgc gcccgcggaa ggcgg
//
LOCUS       DATBSW010000255         6950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_800212, whole genome shotgun sequence.
ACCESSION   DATBSW010000255 DATBSW010000000
VERSION     DATBSW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800212"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VIF63_07170"
     CDS             complement(<1..534)
                     /locus_tag="VIF63_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8239200.1"
                     /translation="MTTDVLPSDRISPEQWAHAHDELVESLRDLIRIKSINPPDPAGP
                     ELDAARFLGQALGDAGILATVYEPVPGRGSVVARLRGDGTGGEPLLLLSHLDVVPAPP
                     ESWTHDPFAGDIADGWLYGRGAVDMKDLTAMELQVMRLLASEARAAGRDPASDPIPGL
                     RRDVLFASTADEEAGGLA"
     gene            complement(536..787)
                     /locus_tag="VIF63_07175"
     CDS             complement(536..787)
                     /locus_tag="VIF63_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239201.1"
                     /translation="MTPQDAGELAGELPADPDAYDSPRAAQARARGLAAPYIAGGLDP
                     EIEATRRSERRYVRLLIAMIVIVVLAGFVLGYLANLLRF"
     gene            complement(784..1734)
                     /locus_tag="VIF63_07180"
     CDS             complement(784..1734)
                     /locus_tag="VIF63_07180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239202.1"
                     /translation="MLEVRAATSDEDLAHIARIVGAVTPDNTTSVSEMRWQEEHYPGG
                     RRFIAWLDGEAVAGGGVGRVYVYPPEFPGLWGNITVLPEHRRKGVGSAMYVAISEVAR
                     AAGKTLLVGRTTSDRPEAIAWMEHRGFEEHERMKVVRLALVGVPLPALEVPEGVELTS
                     LETRPDLVEGVYQVAQEALPDIPGDGPVAPDTIEEFVMRDVDRAAVPQGGFAVAIEKD
                     SGRVVGYANLLSPPGTSKIAWHGMTAVAREWRGRGLARALKVSTIHWAAANGLEGLDT
                     ANDIENAPMRAVNRRLGYEPQPDEIYYRGPLAPVRESSRA"
     gene            complement(1745..3526)
                     /gene="ilvD"
                     /locus_tag="VIF63_07185"
     CDS             complement(1745..3526)
                     /gene="ilvD"
                     /locus_tag="VIF63_07185"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8239203.1"
                     /translation="MTNLPYGRPTDGAKRHSHALTDGPDRAPARAMLKAIGFTDDDLA
                     RPLVGVATTWIETMPCTYNQRRLAQFVKEGIRAGGGTPMEFNTIAVSDGVSMGTEGMK
                     ASLISRDVIADSIELVVRGHLLDGVVCLVGCDKTIPGAAMAMARLDVPGLVLYNGTIY
                     PGEHNGKDLTVVSVFEAVGAYRAGKMSLEELYEIESAACPGAGACGGQFTANTMSMVM
                     EFLGLSPAGLNAIPAEDPDKDAAARQAGELAMTLVRHDIRPSSIVTKASIENAIAAVA
                     ATGGSTNAVLHLLALAHEFNLPLTIDEFDTIAARTPIVGDMMPGGRFTASDLYDAGGI
                     SLVMRELLKRDLVHGEEKNVDGRTMAKIGAAAVATEGQQVVRSIDDPLKPTGGLAILR
                     GSLAPEGCVVKLAGHERLHHRGPARVFDSETACFEAVKNRQIHEGDVVVVRYEGPVGG
                     PGMQEMLSVTAALQGEGLGGSVALLTDGRFSGGTHGLMIGHVAPEAALGGPIALVAEG
                     DEIVIDVEARRLDLDIPDAVLAERRAAWQPPVPRYLGGVMAKYAALVSSASEGAVTTG
                     RRMAEALGRPGATSVAIVAESSPITGG"
     gene            complement(4008..4295)
                     /locus_tag="VIF63_07190"
     CDS             complement(4008..4295)
                     /locus_tag="VIF63_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239204.1"
                     /translation="MSAASAPNPAPLTVTVSPTATIVGAIELIEGTREAGSKSVSEFA
                     EPRDEDTATLPADAPAGTSATIRFALSVVTAAVVPPNVTVLEPASVPNPVP"
     gene            4546..>6950
                     /locus_tag="VIF63_07195"
     CDS             4546..>6950
                     /locus_tag="VIF63_07195"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEY8239205.1"
                     /translation="MQGTAGRRLTVAVPTYENLGTVLLYDTRGRSMPLASPLTPAGTT
                     SGILPVSGTYTVLIDNSFGSLPGSAEIAVRETTDVEATATVDGGPTRVTTDSAGQHAI
                     VYFDAEAGDRVRVAGGPFPALTSVTLVDPYGMALATAAAQSSESAPLFEPFDLNADGR
                     YFLDLDPDRDAVGAMTIEISGAAGALGRDGGGGSAAGSHDAVSPTRATSQCPAGPVAV
                     PLSEALPTTWRPSDEEVWRPTAANLAGDWSTHRQPSPWEWTAALMATPLTTAVSGRVL
                     RLDGLPLAGVRVTADGAATCTDASGRFLLGPLSAGPHELVIDGSPAASPGRAYGTVEL
                     GALVVGGQTNVLPFTIWMSRVDTGSVVRVPKHLRTELVVTNPSMPGLELRIPAGSHLV
                     DSEGQPVTEMGLTPVPIDRPPFPLPPGAAFPMYFTVDPHGVVVAPEGATVVYPNVTRK
                     APGSPASFWYYEPEEGWENYGGGSVAEDGRSVVPAHGIALDDLGFSAFIPGESLPVNG
                     PVNGQGCCATAGEPVDLASGVFLQQNADLAIADVMPLDVGRTYRSADPTIRSFGLGTT
                     HQYNMYLSAAANTPLYLVLPDGTGVPYKSIGPGPFFGNLYEHRASPTRFHGSRIFFAA
                     DLPQGGWWLVMRDGSRFLLGQHAALQEVRDRNGNAITITRVGGAEAPASQVTSPGGRW
                     ISLTYDDPAHATLVTSASDVAGREVGYAYDERQRLTAVTDADGGVTRYEYDEVGNMTS
                     VTTPRGIALLRNEYDDGGRVVRQTQADGGVHAFAYTVTGERISESLYTDPLGHRRRIS
                     FNE"
BASE COUNT         1042 a         2416 c         2395 g         1097 t
ORIGIN      
        1 ggcaagcccg ccggcctcct cgtccgccgt cgacgcgaag aggacgtcgc gacggagacc
       61 cgggatcggg tcggacgcag ggtcgcgccc ggccgcccgc gcctcgctcg cgagcaggcg
      121 catgacctgg agctccatcg cggtgaggtc cttcatgtcg accgcgccgc gcccatacag
      181 ccagccgtcc gcgatgtcgc cggcaaacgg gtcgtgggtc cagctctcgg gcggcgcggg
      241 gacgacgtcg aggtgcgaga gcaggagcag cggctcgccg ccggtgccgt caccgcgcag
      301 gcgcgccacg acggagcccc gcccgggcac gggctcgtag accgtggcca ggatgccggc
      361 atcgccgagg gcctggccga ggaagcgggc cgcgtccagc tccgggccgg ctggatccgg
      421 cgggttgatc gacttgatcc ggatcaggtc ccgcagcgac tcaacgagct cgtcgtgagc
      481 gtgcgcccac tgctccggcg agattcgatc ggagggcagg acgtcggtgg tcacgtcaga
      541 accggaggag gttcgcgaga tagcccagca cgaagccggc gagcacgacg atcacgatca
      601 tcgcgatcag gagccggacg tagcggcgct cggatcgccg cgtggcctcg atctcggggt
      661 cgagcccgcc tgcgatgtac ggcgcggcga ggccgcgtgc ccgcgcctgg gcggcgcgag
      721 gggagtcgta ggcatcggga tcggcgggta gctcgccggc tagctcgccg gcgtcctgcg
      781 gggtcacgcc ctggagctct cgcgaaccgg cgcgagcggg ccgcggtagt agatctcgtc
      841 gggctgcggc tcatagccca ggcgtcggtt gaccgcgcgc atcggcgcgt tctcgatgtc
      901 gttggccgtg tcgaggccct cgaggccgtt cgcggcggcc cagtggatcg tcgagacctt
      961 caacgcacgg gcgagcccgc gaccgcgcca ctcgcgggcg accgcggtca tcccgtgcca
     1021 ggcgatcttc gaggtgccgg gtggggagag caggttggcg tagccgacca cccgcccgct
     1081 gtccttctcg atcgcgaccg cgaagccgcc ctgcgggact gcggcccgat ccacgtcacg
     1141 catcacgaat tcctcgatcg tgtccggcgc gaccgggcca tcgccgggga tgtccggcag
     1201 cgcctcctgc gcgacctggt acacgccctc gacgaggtcc ggcctcgtct cgagggaggt
     1261 gagctcgacg ccctcgggca cctcgagcgc cggcagtggc acgccgacga gggcgaggcg
     1321 gacgaccttc atgcgctcgt gctcctcgaa gccccgatgc tccatccacg cgatcgcctc
     1381 cggccgatcg gatgtggtcc ggccaaccag gagggtcttg cccgccgccc gggcgacctc
     1441 ggagatggcc acatacatcg cgctgccgac gcccttccgg cgatgctccg gcagcaccgt
     1501 gatgttgccc cacaggcccg ggaactcggg cgggtagacg tacacccggc cgacgccgcc
     1561 accggccacg gcctcgccat cgagccaggc gatgaaccgg cgcccgcccg ggtagtgctc
     1621 ctcctgccag cgcatctcgc tgaccgacgt cgtgttgtcg ggagtgacgg cgccgacgat
     1681 ccgcgcgata tgggcgaggt cctcgtcgga agtcgcggca cggacttcga gcatcagccg
     1741 agcgtcagcc tcctgtgatc ggcgacgact cggccacgat cgccaccgat gtcgcacccg
     1801 gtcgcccgag ggcctcggcc atccgcctgc cggtcgtcac cgcgccttcg ctcgccgacg
     1861 acacgagcgc cgcgtacttc gccatcaccc cgccgaggta gcgcgggacc ggcggctgcc
     1921 aggcggcgcg ccgctcggcg agcacggcgt cggggatgtc gaggtcgagc cggcgcgctt
     1981 cgacgtcgat gacgatctcg tcgccctcgg cgacgagcgc gatcggccca ccgagcgccg
     2041 cctcgggtgc gacgtggccg atcatcaggc cgtgggtgcc gccggagaag cggccgtcgg
     2101 tgagcagcgc cacggatcca cccaggccct cgccctggag cgccgcggtc acgctcagca
     2161 tctcctgcat gcccggtccg ccgaccgggc cttcgtagcg aaccacgacg acatcgccct
     2221 cgtggatctg ccggttcttg accgcttcga agcacgccgt ctccgaatcg aagacgcgcg
     2281 ccgggccgcg gtggtggagt cgctcgtggc cggcgagctt gacgacgcag ccctcgggcg
     2341 cgagcgagcc gcgcaggatc gcgaggccac ccgtcggctt gagcgggtcg tcgatcgagc
     2401 ggacgacctg ctggccctcg gtcgccaccg ccgccgcgcc gatcttcgcc atcgtccggc
     2461 cgtcgacgtt cttctcctcg ccatggacga ggtcccgctt gagcagctcg cgcatgacca
     2521 gcgagatgcc gcccgcgtcg tacaggtccg aggcggtgaa gcggccgccc ggcatcatgt
     2581 cgccgacgat cggggtgcgc gccgcgatcg tgtcgaactc gtcgatcgtc agcggcaggt
     2641 tgaactcgtg ggcgagtgcc aggaggtgga ggacggcgtt cgtcgagcca ccggtcgcgg
     2701 caacggccgc gatggcgttc tcgatcgagg ccttcgtgac gatgctcgac ggccggatgt
     2761 cgtgtcggac gagcgtcatc gcgagctcgc cggcctggcg cgccgcggcg tccttgtcgg
     2821 ggtcctcggc cgggatcgcg ttcagcccgg ccggtgagag gccgaggaac tccatgacca
     2881 tcgacatcgt gttcgccgtg aactggccgc cgcacgcgcc cgcgccgggg caggccgcac
     2941 tctcgatctc gtacagctcc tcgaggctca tcttgcccgc gcgatacgcg cccaccgcct
     3001 cgaagacgga gaccacggtc aggtccttgc cgttgtgctc gcccggatag atggtgccgt
     3061 tgtagaggac gaggccgggg acatcgagcc gggccatcgc catcgccgcg cccgggatcg
     3121 tcttgtcgca gccgaccagg cagacgacgc cgtcgagcag gtgcccgcga acgacgagct
     3181 cgatcgagtc ggcgatcaca tcgcggctga tcaggctcgc cttcatgccc tcggtgccca
     3241 tcgagacgcc gtccgacacc gcgatcgtgt tgaactccat cggcgtgccg ccgccggccc
     3301 ggatgccttc cttcacgaac tgcgccagcc ggcgctggtt gtacgtgcag ggcatcgtct
     3361 cgatccacgt cgtggcgacg ccgacgagcg gccgggcgag gtcgtcatca gtgaacccga
     3421 tggccttgag catcgcccgc gccggcgccc gatccgggcc gtcagtcagg gcgtgggagt
     3481 ggcgcttggc gccgtctgtc ggtcgaccgt agggcagatt ggtcatgacg cggccatctt
     3541 aggcaactgc ggcgcgatcg tcggctttcg cggtccgttc gtgtgccgga cgggcaggtt
     3601 tcggagcaag tgcctgccgc atactcatct cgagggacgt tccgggtcgc gaaaggttca
     3661 tacgcggctg gggaggatcg acgcatgaag cgtcagttgg ctgtggctgc cctgagtctc
     3721 gtcatggtct cgtcggcgtg cgagccgacg cccgtcactc catcagcgca tccaatcacc
     3781 tcggctccgc cgatcactcc aggcccaccg atcgtcgcgt cgacggcgac ctatgtccac
     3841 gacgcgcttg gccgcctcgt agcggtgatc gacgagacac agggcgtcgc gcgctatgca
     3901 tacgacgccg tcggcaacat cacctccatc agccgaccca gccccgacgt ggtctcggtc
     3961 atcgaagtga cgccagattg gggacccgcc ggcaccaagg tcaccattta cgggacagga
     4021 ttcgggacgg acgccggctc caacaccgtc acgtttggcg gtaccacggc ggcggtcaca
     4081 accgagagcg cgaaccggat cgtggccgag gttccggccg gcgcgtcggc cggcaaggtt
     4141 gccgtgtcct cgtcgcgcgg ttcggcgaac tcggacaccg acttcgaccc cgcttcgcga
     4201 gtgccctcga tcagctcgat cgcgccgacg atcgtggccg tgggcgacac cgtcaccgtc
     4261 agcggggcgg gatttggcgc cgaggcggcg gacaacgatc tcgccttcaa tcggacgcgg
     4321 gttcgcatcg acacgaccgg cgccgatcgc ctgaccggtt cggtcgtcca cgagacgggc
     4381 tcaggcccgg tgtcactcgc aacgatctac ggagaagtag tcggcccgga cctgtttgtc
     4441 gttcccgcgc cgtacacccc ggcccaggtc ggcgggaccg cgcgcctcga tccggggtcg
     4501 tcccggcgct tcgacgtgcc ggcgagcaac atcaccctca tcgccgtgca gggcaccgcc
     4561 ggtcgtcgac tgaccgtcgc ggtcccgacg tacgagaacc tcgggaccgt cctgctgtac
     4621 gacacccgcg gccgatcgat gccgctcgct tcgccgttga ccccggcagg cacgacgagc
     4681 ggcattctcc cagtgtcagg gacgtacacg gtcctgatcg acaactcgtt cggcagcctg
     4741 cctgggtcgg ccgagatcgc ggtgcgcgag acgacggatg tcgaggcaac tgcaacagtt
     4801 gacggcggac caaccagggt cacgacggac tccgcgggcc aacacgccat cgtgtacttc
     4861 gacgcagagg caggcgaccg cgtgcgagtt gccggcggcc cgttcccggc actgacgtcg
     4921 gtgacgctgg ttgatccgta cggcatggcg ctggctacgg cggcagccca gtcgagcgag
     4981 tccgctccac tctttgagcc cttcgatctc aatgccgatg ggcggtactt cctcgacctc
     5041 gatcctgatc gcgacgcggt cggcgcgatg accatcgaga tttcgggtgc ggcgggcgcg
     5101 ctcgggcgcg acggtggagg aggctccgcg gccggatccc acgacgcggt ctcgccgacg
     5161 cgcgccacat cgcagtgccc agctgggccg gtggcggttc ccttgagcga ggcgcttccc
     5221 accacctggc ggccttccga tgaggaggtt tggcgtccca cggcggccaa cctggcgggc
     5281 gattggtcga cccatcgtca accgtcgccc tgggagtgga ccgcagccct catggccacg
     5341 ccgttgacga cagcggtctc gggccgcgtc ctgcggctcg acggtctgcc cctcgccggc
     5401 gtgcgagtca cggcggatgg agcggcgacc tgcaccgatg cttcgggccg gttcctgctg
     5461 gggccgctga gcgcgggtcc ccatgagctg gtcatcgatg gctcaccggc ggccagcccg
     5521 ggccgggcat acggaaccgt tgagcttggc gctcttgttg tcggcggaca gaccaacgta
     5581 ttgccattca cgatctggat gtctcgagtc gacacgggaa gcgtggttcg cgtcccgaag
     5641 catctgcgca ctgagctcgt cgtgacgaac ccatccatgc caggcctcga gctgcgaatt
     5701 ccggccggaa gccacctggt cgacagcgag ggccagccgg tcacggaaat gggcctcacg
     5761 ccggtgccaa ttgaccgtcc accattcccg ttgccgcccg gagcggcctt tccgatgtac
     5821 ttcacggtgg atccacacgg agtggtcgtc gcccctgagg gggcaacggt cgtctatccg
     5881 aacgtcacgc ggaaggctcc gggttcgccg gcttcgttct ggtactacga acccgaggaa
     5941 ggttgggaga actacggggg cggctcggtc gcagaggacg ggaggagcgt cgttcccgcc
     6001 cacggcatcg ccctcgatga cctcggattc agcgcgttca ttcctggtga gagcttgccc
     6061 gtcaacggtc cggtcaacgg gcaaggatgt tgcgccaccg ccggggaacc cgtggacctt
     6121 gcgagtggtg tgttccttca gcagaacgct gacctggcaa ttgccgacgt catgccactc
     6181 gacgtcggac ggacgtatcg atctgccgat cccacgatcc ggtcgttcgg gttggggacg
     6241 acgcaccagt acaacatgta tctctcggcg gccgccaaca cgccgctgta cctggtgctg
     6301 cccgatggca ctggcgttcc atacaagtcg atcggtccag ggcccttttt cggcaatctc
     6361 tatgaacatc gggcgagtcc cacccggttc catggatcgc gcatcttctt tgcggctgac
     6421 ctgccgcaag gcggctggtg gctcgtcatg cgagacggtt cccgcttcct gctggggcag
     6481 cacgctgcgc tgcaggaggt ccgcgaccgc aatggcaatg ccatcacgat tacgcgggtt
     6541 ggtggtgcag aggcaccggc cagccaggtg acgtcgccgg gcggccgatg gatcagcctc
     6601 acctacgacg acccggcgca tgcaacgctc gtgacatccg cctccgatgt cgccggccga
     6661 gaggtcggct atgcgtacga cgaacgccag cgcctgacag cggtaacaga cgctgacggt
     6721 ggggtgactc ggtacgaata cgacgaagtc ggcaacatga cgtcggtcac gactccgcgt
     6781 ggcatcgcgc tccttcggaa cgagtacgac gatggcggcc gggtcgtccg tcagacccag
     6841 gcggacggtg gcgttcacgc atttgcgtac acggtgacgg gtgagcgaat cagcgagagc
     6901 ctctacacgg atccgctcgg tcaccgccgc cgcatctcgt tcaacgagga
//
LOCUS       DATBSW010000256         9104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_448029, whole genome shotgun sequence.
ACCESSION   DATBSW010000256 DATBSW010000000
VERSION     DATBSW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448029"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VIF63_07200"
     CDS             complement(<1..552)
                     /locus_tag="VIF63_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239206.1"
                     /translation="MAAMRDVTLVASGDLRETANRLGWPAQAELERNVGAAFGRHGAR
                     VTRAHPVDATRGHGFISSQRMGMDVFASIDRDAPLVVAEAVWQYSHHVLPGLRRHRGP
                     ILTVANWSGTWPGLVGMLNINASLTKMGRDYSTIWGEDLTDPFADAAIGAWVADGRIV
                     HDTSHVRDLDPAALRPEATTLGRR"
     gene            complement(562..1815)
                     /locus_tag="VIF63_07205"
     CDS             complement(562..1815)
                     /locus_tag="VIF63_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239207.1"
                     /translation="MAAQPSVQAHNWRMVQLDSFFIGVINASSTFLPVFLLRLGASAN
                     DVGLLTALPALTAFAFSIPLGRWMQRRRNIVPWYSGLRLVAWSSYAAMAAVAFLLPRE
                     QAIPALLAVWALSSLPSTAALVGFAMVMDGAAGPGGRFDLLGRRWAIAGVVATAAGVL
                     GGQLLSIFPFPMNFEILLVIVSLAGLGSFFQSRRIVIPDQAPVPSAAHVPTRDRLVGL
                     WHLIVSNRQFIRFQLRGFVYVASIGMSLPVLPLFYVHEVNAPDAWIGVIGAAASAGSV
                     LGYLVARQLALRRGAAMTLLPSMLVAALAPAVLSMIGWLPAVAAIAFVAGVAGAGAQL
                     ALFDQMMRRIPHEHGVTFSSVDQSLSNLAIMIAPNVGGILAIAIGIRSTLLVVAALGV
                     AAFVLVALDVRATRNAEARAATQPA"
     gene            1898..3001
                     /gene="cax"
                     /locus_tag="VIF63_07210"
     CDS             1898..3001
                     /gene="cax"
                     /locus_tag="VIF63_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEY8239208.1"
                     /translation="MPRIDRRTWLIFGGYAVAFVIVAIANVLALPPLIRFAVAVVALA
                     GGAIAVGEATERLSQRLSPAATGVVQSVLGNLPELFLAIFALQAGLVEVVAAALVGSV
                     LGNALFVLGLATLVGGLRHGKLTFSAAANRLYATELLLGVAALTIPFLATQPGAPDFG
                     HAEELSAVVAIVLLVLFAVSVPIALRLSTEGPGTRPHVPEDDDPATPDVPRPLVPTLL
                     LLGISAASAAFVADWFVDALEPAMATIGMSEAFAGLIVVAIASNAVENLAGVSSAAKG
                     KGDLAMSLILNSALQIVLFLTPVIVLLSFFVAPVPLTLIISPLLLGTLAVSVLLVIFI
                     VTDGEANAFEGAMLLGLYVIIAAAVWWGPRIGS"
     gene            3037..3924
                     /locus_tag="VIF63_07215"
     CDS             3037..3924
                     /locus_tag="VIF63_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY8239209.1"
                     /translation="MTTWQDLVQGGGPIVADGAMGTMLMANGLEFGDPPELWNLEHPE
                     IIRRVQRGYLDAGAQVLLTNTFGGNRLRLELHGRQDRVDQLNRTAAVLAKVEANAADH
                     PAIVAGDIGPSGQLMEAIGGTLTPEIAREVFAEQARALAAGGVDVMWIETMSDVDEAV
                     AAVRGAKDAAPDAPVIATMSFDTRGRTMMGVTPEQAAAALLEAGAAAIGGNCGNGPDE
                     LVPVIERIRAAFPDAIIVAKGNVGMPQLVGMAVEYETTPQTMAEFARRFVDAGANIVG
                     ACCGSTPPHLKAMAEALRT"
     gene            complement(4000..5334)
                     /locus_tag="VIF63_07220"
     CDS             complement(4000..5334)
                     /locus_tag="VIF63_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239210.1"
                     /translation="MLMFAGAVAPVAGWANGPDGGNGFGTHDWILDQALRFLDAEGVN
                     TSWVDRTRALLATDDPDKIEVAADSSREIEHVYTAGGRRGGAVDRITEHYATMLRAYA
                     AGDYDKASYQLGMLAHFYGDILQPYHTSRDAIGQTTKHIAYELLVDKGHRKTTDSPGW
                     SILGTSWALKDITNIRTTALAAATYSRGKYTDLAANFDANDTALSSTANTITRDVLRR
                     ASGDLANIVQSVAKGVGNAPAAATVTLTLRWRGVKTNEVSERLDVNVFDVNGKPIEGV
                     LGTVSWPQPDGSKLKMPFWTDEFGNGHIYGKVGSNTYMAHQTVTVTVTTNAKTLTKST
                     WWYRTPRLADSTAGYSTSISDSTVVNGQTVTLTTTAKTSTGSPIVGLLITYSWYVGST
                     TITTTAYTDSTGKAKSSYKVLSTTTHSTITVTARTSAYSINRSSTKTFHRVD"
     gene            5528..6265
                     /gene="lipB"
                     /locus_tag="VIF63_07225"
     CDS             5528..6265
                     /gene="lipB"
                     /locus_tag="VIF63_07225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8239211.1"
                     /translation="MEAPHGIAASWLGRVDYRSAWAVQKGLVAHRADGEIGDRLLLLE
                     HDAVLTLGRQADDAHVLASPRELRRRGIEVIRVERGGEVTYHGPGQLVAYPIIRLGDR
                     GLLVRPLVEALEAAMIETCAHLGVEAIRRDGHPGCWIDGGPARPHRKIGALGLRIERG
                     VSYHGIALNIAPDLRDFDLIDACGMPGVVSTSIAEELGRTAEPPTTEAVERAGKVFAE
                     AFATAIRAPLAWVEAPVPGEAQVPVEA"
     gene            6317..7309
                     /locus_tag="VIF63_07230"
     CDS             6317..7309
                     /locus_tag="VIF63_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239212.1"
                     /translation="MSAGLFELRKDPITGWWVATIVDRDFDRSRFARAAAPIDDGGDC
                     QNCRLPSGDGVRMRVLKDFAFHVIGTEQERDEIDRGITQMTVSEARAAGSWRTIAAPP
                     TEHRALAHVGEVAEQMLLLARDAVAEATSLAQTDYLQVVQNWGAQAGARTNHLCLDLY
                     DLPLIPHRVAEELGGAARFVIREGECPWCRLVREESRTRERLVWEDATTVAFAPWGSR
                     SPFEVWIVPRVHQADFGRADDAAVRATASALRNVLGGLGVSLDGPPYNLVLHTAPLRE
                     RVDATFHWHWEVHPRLREIAGLELGTGLPVNPVSPEAAVEELLERAGARAEPGA"
     gene            7425..8060
                     /locus_tag="VIF63_07235"
     CDS             7425..8060
                     /locus_tag="VIF63_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8239213.1"
                     /translation="MLERSATPTAHIALGRPVSRTWEQDAARTFVDNLFTKHHTEIYA
                     YLVRMLRDSELAADLTQDAFIKAYKNYDTLEKDENARAWLYQIAHRVALDELRRRKII
                     RMIPWTGESHGAAPSAERIAMDLRLSGPLERALASIPERQRAALLLAELHDLTGVELA
                     AAMGVSHVAARAILTRARESLRRALAAEKDAERAAEADRDARFAAGAGSDR"
     gene            8057..>9104
                     /locus_tag="VIF63_07240"
     CDS             8057..>9104
                     /locus_tag="VIF63_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239214.1"
                     /translation="MTLPFRRRHHDDEGTHDRARALTSREMIESLPEEETAWLTRHLA
                     ECGECTQDREAYLADRALLRSLRDNTPEPPRDLWARTSAALDREARSHRGTGARARRD
                     ARAGRRQLGRPYGAAAGMLVVLIVVALASIKPIVPPSATPGASNVAVATPPAAEPTQI
                     IVTAGRVGWIRAANDGQWELILADVKAVCPRSRPNCGQLVEDDPGRTINLGSAPTGGF
                     TISENEQQLVIERNAEGAAPGRILVVPIPPASPAVTPAPVDTPKPVDTPKPIDTPKPI
                     DTPTPIDTPSPADTPTPAPPTGEPVSPAVPTPVPTSTTPDGTIEIASGVTMVGEAAYS
                     SDSKWLAFSARPSDG"
BASE COUNT         1360 a         3236 c         3184 g         1324 t
ORIGIN      
        1 tcgccggccg agggtggtcg cctcggggcg cagggccgcc ggatcgaggt cgcggacatg
       61 ggacgtgtcg tggacgatcc ggccatcggc cacccaagcc ccgatcgcgg cgtccgcgaa
      121 cggatcggtc agatcctcgc cccagatggt gctgtagtca cggcccatct tcgtgaggga
      181 ggcgttgatg ttcagcatgc cgacgagacc tggccaggtg ccgctccagt tggcgaccgt
      241 caggatcggt ccgcgatgcc ggcgcaggcc gggcagcacg tggtggctgt actgccagac
      301 ggcctcggcg acgacgagcg gggcatcccg gtcgatcgac gcgaacacgt ccatgcccat
      361 gcgctggctc gagatgaacc cgtgcccccg cgtggcatcg accggatggg cgcgcgtgac
      421 cctggcgccg tggcggccga acgccgcccc gacgttgcgc tccagctccg cctgcgccgg
      481 ccagccgagc cggttcgcgg tttccctgag gtcgcccgac gcgaccagcg tcacgtcacg
      541 catcgcagcc atcgccgagc atcatgcagg ttgcgttgcc gctcgcgcct cggcgtttcg
      601 cgtcgcgcgg acatcgagcg cgaccaggac gaaggccgca acccccagcg ccgcgacgac
      661 gagcagggtc gatcgaatgc cgatcgcgat ggcgaggatg ccgccgacgt tcggggcgat
      721 catgatcgcg aggttcgaca gcgattggtc taccgagctg aacgtgacgc cgtgctcgtg
      781 ggggatccgc cgcatcatct ggtcgaacag ggcgagctgc gcgccggcgc cggccacccc
      841 cgccacgaat gcgatcgccg cgacggccgg cagccacccg atcattgaca ggaccgccgg
      901 cgcgagcgcg gccacgagca tggatggcag gagggtcatc gccgcgccac ggcgcagggc
      961 cagctggcgg gcgaccaggt agccgaggac gctgcccgcc gacgccgccg cgccgatgac
     1021 gccgatccac gcgtccgggg cattgacctc gtgcacgtag aagagtggca ggacgggcag
     1081 cgacatcccg atgctcgcga cgtagacgaa gccgcgcagc tggaaccgga tgaactgccg
     1141 gttggacacg atgaggtgcc acaggccgac cagccggtcc cgcgtcggga catgtgcagc
     1201 gctgggcacc ggcgcctggt ccgggatgac gatgcgtcgc gactggaaga agctgccgag
     1261 gccggcaagc gacacgatca ccagcaggat ctcgaagttc atcgggaacg gaaagatgct
     1321 caggagctga ccgcccagca ctccggccgc cgtcgccacg acgcccgcaa tcgcccagcg
     1381 ccgcccgagc aggtcgaacc ggccgcctgg cccggccgcg ccgtccatga ccatcgcgaa
     1441 cccgacgagc gccgcggtcg agggcagcga cgagagtgcc cagaccgcaa gcagcgcggg
     1501 gatcgcctgc tctctcggca gcaggaacgc aaccgcggcc atcgccgcgt agctggacca
     1561 cgcgacgagc cggagcccgc tgtaccaggg cacgatgttc cgtcggcgtt gcatccaccg
     1621 cccgagcgga atcgagaacg cgaaggcggt cagcgccggc agcgcggtga gcaggccgac
     1681 gtcgttcgcc gaggcaccca accggagcag gaacacgggc aggaacgtgc tcgacgcgtt
     1741 gatcacgccg atgaagaagg agtcgagctg gaccatccgc cagttgtggg cctgcaccga
     1801 cggttgtgcg gccaagtcca cctcctgtgc gacaagatgc gggcgggatg cgctggcggg
     1861 cgaggcgcgc tcggaatcac cgatgatacc gaccgacatg ccgcgcatcg accgacgcac
     1921 ctggctcatc ttcggcggct atgccgtcgc gttcgtgatc gtggccattg ccaacgtgct
     1981 cgcactcccg ccgctcatcc gcttcgcggt ggcggtcgtc gcgcttgccg gcggcgcgat
     2041 cgccgtgggc gaagccacgg agcgcctgtc ccagcgcctg tccccggcgg ccaccggcgt
     2101 cgtccagtcc gtgctcggca acttgcccga gctgttcctc gcgatcttcg cgctccaggc
     2161 gggcctggtc gaggtcgtgg ccgccgcgct cgtgggctcg gtgctcggca acgccctgtt
     2221 cgtcctcggc cttgcaaccc tcgtcggcgg cctccgccac ggcaagctca cgttctccgc
     2281 ggctgcgaac cggctctacg cgaccgagct gcttctcggc gtcgccgcgc tgaccattcc
     2341 gttcctggcg acgcagcccg gcgcgcccga cttcggccat gccgaagagc tgtccgcggt
     2401 cgtcgcgatc gtgctcctgg tgctcttcgc ggtctcggtc ccgatcgcac ttcgcctctc
     2461 gaccgaaggg cccggaaccc ggccacacgt gccggaggac gacgatcccg ccacgcccga
     2521 cgtcccgcgg ccgctcgtcc cgacgctgct gctgctcggc atctcggccg cctcggctgc
     2581 gttcgtcgcg gactggttcg tggacgcgct cgagccggcg atggcgacga tcggcatgag
     2641 cgaggccttt gccgggctga tcgtggtcgc gatcgccagc aacgccgtcg agaacctcgc
     2701 gggagtgagt tcggccgcca agggcaaggg cgacctggcg atgagcctga tcctcaactc
     2761 ggctcttcag atcgtgctgt tcctgacccc cgtgatcgtg ctcctgagct tcttcgtcgc
     2821 gccggttccg ctgacgctga tcatcagccc gctgctcctc ggcaccctcg cggtcagcgt
     2881 gctgctcgtg atcttcatcg tcaccgacgg cgaggccaac gcgttcgagg gcgcgatgct
     2941 cctcggcctg tacgtgatca tcgccgccgc cgtctggtgg ggcccgcgaa tcggctcgta
     3001 gcggcgtcca gggatccgcg gcacaatctc gcgaccatga cgacctggca ggatctcgtg
     3061 caggggggcg ggccgatcgt tgccgacggc gcgatgggca cgatgctcat ggccaacggg
     3121 ctcgagttcg gtgatccacc cgagctgtgg aacctcgagc accccgagat catccggcgc
     3181 gtccaacgcg gctacctcga tgccggcgcc caggtgctcc tcacgaacac gttcggcggc
     3241 aatcgactgc gcctcgagct gcacggccgc caggaccggg tcgaccagct gaatcgaacc
     3301 gccgcggtgc tcgccaaggt cgaggcgaac gccgcggacc acccggcgat cgtcgccggc
     3361 gacatcggcc cgagcggcca gctcatggaa gcgatcggcg gcacgctcac gcccgagatc
     3421 gcccgggagg tcttcgccga gcaggcgcgc gccctcgccg caggcggcgt cgacgtcatg
     3481 tggatcgaga ccatgagcga cgtcgacgag gccgtcgccg cggtccgcgg cgcgaaggac
     3541 gcagcccccg acgccccggt catcgccacg atgagcttcg acacccgcgg ccggacgatg
     3601 atgggcgtca cgcccgagca ggccgccgca gcgctcctcg aagccggcgc cgcagccatc
     3661 ggcgggaatt gcgggaacgg tcccgacgag ctcgtgccgg tcatcgagcg gatccgcgcc
     3721 gcgttcccgg acgcgatcat cgtggccaag ggcaacgtcg gcatgccgca gctcgtcggg
     3781 atggcggtcg agtacgagac gacgccgcag acgatggccg agttcgcgcg gcgcttcgtg
     3841 gatgccggcg ccaacatcgt gggcgcctgt tgcggcagca cgccgccaca tctcaaggca
     3901 atggccgagg cgctccgaac ctgacgggtg agcgcggtgc gccgggtgag cgcggtgcgc
     3961 cggatacgcc ggggtgggtc gaccccgact ccgaccgggt cagtcgaccc ggtggaacgt
     4021 cttggtggac gagcggttga tgctgtacgc ggacgttcgc gcggtgaccg tgatggtcga
     4081 gtgcgtcgtc gtgctcagga ccttgtagct cgacttcgcc ttgccggtgg agtccgtgta
     4141 cgcggtcgtc gtgatcgtgg tcgagcccac gtaccagctg taggtgatga gcaggccgac
     4201 gatcggcgag cctgtgctcg tcttcgcggt ggtcgtcagg gtgaccgtct ggccgttcac
     4261 gaccgtcgag tcgctgatgc tcgtcgagta gcccgccgtg ctgtcggcga ggcgcggcgt
     4321 ccgataccac caggtcgact tggtgagggt cttcgcattg gtcgtcacgg tgaccgtcac
     4381 ggtctggtgg gccatgtagg tgttcgaccc gaccttgccg tagatgtggc cgttgccgaa
     4441 ttcgtccgtc cagaacggca tcttgagctt cgagccgtcc ggctgcggcc agctgaccgt
     4501 cccgaggacg ccctcgatcg gcttgccgtt gacgtcgaag acgttgacgt cgagtcgctc
     4561 ggagacctcg ttcgtcttca ccccgcgcca gcgcagcgtg agcgtcacgg tggcggcggc
     4621 cggcgcgttg ccgacgccct tggccaccga ctggacgatg ttcgccaggt cgcctgaagc
     4681 gcgccggagg acatcgcgcg tgatcgtgtt ggccgtcgag ctcagggccg tgtcgttggc
     4741 gtcgaagttg gcagccaggt ccgtgtactt gccccgcgag tacgtcgcgg ccgccagggc
     4801 ggtcgtgcgg atgttggtga tgtccttgag ggcccagctc gtgcccagga tcgaccagcc
     4861 gggcgaatcg gtggtcttgc ggtggccctt gtcgacgagc agctcgtagg cgatgtgctt
     4921 ggtggtctgg ccgatcgcgt cccgcgacgt gtggtacggc tggaggatgt cgccgtagaa
     4981 gtgggcgagc atgccgagct ggtagctcgc cttgtcgtaa tcacctgcgg cgtaggcacg
     5041 gagcatggtg gcgtaatgct cggtgatgcg gtcgaccgcg cccccgcgcc gaccgccggc
     5101 ggtgtacacg tgctcgatct cgcgcgagga atcagcggcg acctcgatct tgtcgggatc
     5161 gtcggtggcg aggagggcgc gagtgcggtc gacccaggac gtgttcacgc cctcggcgtc
     5221 gaggaaccgc agcgcctggt cgaggatcca gtcgtgggtg ccgaagccat tgccgccgtc
     5281 aggcccgttg gcccaaccgg cgacgggcgc gacggcgccc gcgaacatca gcaacgaggc
     5341 caaggtggcg gttgcgaggc gactcagact gggggcacga gatcgcatca gtgattcctg
     5401 tgtaacgggt acagggcaca tgatcgacgc cgtcggcgcc tgcggacagt ggaccaatgg
     5461 tcccaaaaag tgccgacgcg caccggttga gaactgtccg cgaagggtcg tttgctatcg
     5521 tctgaacgtg gaagcaccgc acggcatcgc cgcgtcctgg ctcggtcggg ttgattatcg
     5581 cagcgcgtgg gcggtccaga aggggctcgt cgcgcaccgt gcggacggcg agatcggcga
     5641 tcgcctgctc ctcctcgagc acgacgcggt gctgaccctc ggccgccagg cggacgacgc
     5701 ccatgtcctg gcctcgccgc gcgagctgcg gcggcgtggg atcgaggtga tccgggtcga
     5761 gcgcggcggc gaggtgacct accacgggcc cggccagctc gttgcctacc cgatcatccg
     5821 cctcggcgac cgcgggctgc tggttcggcc gctggtcgag gcgctcgaag cggcgatgat
     5881 cgagacctgc gcccacctcg gcgtcgaggc gatccggcgg gacggccatc cggggtgctg
     5941 gatcgacggc gggccggccc ggccgcaccg gaagatcggc gcgctcgggc tgcgcatcga
     6001 acgcggcgtc agctatcacg ggatcgcgct caacatcgcg cccgacctgc gcgatttcga
     6061 cctcatcgat gcctgcggca tgcccggcgt cgtgtcgacc tcgatcgccg aggagctcgg
     6121 gcgcactgcc gagccgccga cgaccgaggc tgtcgagcga gccggcaagg tcttcgccga
     6181 ggcattcgca accgccatcc gcgcaccgct tgcgtgggtc gaggcaccgg taccgggcga
     6241 ggcacaggtc ccggtcgagg cctgagactc cccaaatgac cccgcgaccc agcgcccaaa
     6301 ccccccgagc gacccgatga gcgccggcct gttcgagctg cgcaaggacc cgatcacggg
     6361 ctggtgggtc gcgacgatcg tcgaccgcga cttcgaccgg agccggttcg cccgtgccgc
     6421 ggcgccgatc gacgacggcg gggactgcca gaactgccgc ctgccatcgg gtgacggcgt
     6481 acggatgcgc gtcctgaagg actttgcgtt ccacgtcatc ggcacggagc aggagcgcga
     6541 cgagatcgac cggggcatca cgcagatgac cgtgtcggag gcccgcgccg cgggcagctg
     6601 gcggacgatc gcggcgccgc cgaccgagca ccgcgccctc gcccatgttg gcgaggtcgc
     6661 cgaacagatg ctcctgctcg cccgcgacgc ggtcgccgag gcgacgagcc tcgcccagac
     6721 cgactacctc caggtcgtcc agaattgggg cgcccaggcc ggcgcgcgga ccaatcacct
     6781 gtgcctcgac ctgtacgacc tgccgctcat cccgcatcgc gtcgcggagg agctcggggg
     6841 tgcggcgcgc ttcgtgatcc gtgaaggcga gtgcccgtgg tgccgcctcg tccgggagga
     6901 gtcgcgcacg cgggagcggt tggtctggga ggacgcgacg accgtcgcgt tcgcgccctg
     6961 gggctctcgc tcgccgttcg aggtgtggat cgtgccgcgc gtccaccagg ccgacttcgg
     7021 ccgcgccgac gacgccgccg tccgtgccac cgcgtcggcc ctgcgcaacg ttctcggcgg
     7081 cctcggcgtg agcctggacg ggccgcccta caacctcgtc ctgcacacgg cgccgctgcg
     7141 cgagcgcgtc gatgccacgt tccattggca ctgggaggtc cacccgcgac tgcgcgagat
     7201 cgccggtctc gagctgggca cgggcctgcc ggtgaacccg gtctcgcccg aggcggccgt
     7261 ggaagagctg ctcgagcggg cgggggcgcg cgcggagccc ggcgcgtgat cgtgcgcgtg
     7321 cgtcacacat gcccaaatcc ggtcgtcgca cgcccggaca ccgccggcct ccatcgatcc
     7381 tcatgggtcg acccggcggc tcccacgttg aaagagtcgc cctcgtgctc gaacgctccg
     7441 cgacgccaac cgcgcacatc gcgctcggcc gcccggtgag ccgcacctgg gagcaggacg
     7501 ccgcccggac gttcgtcgac aacctgttca cgaagcacca caccgagatc tacgcctatc
     7561 tcgtgcggat gcttcgcgac tccgagctgg cggcggacct cacccaggac gcattcatca
     7621 aggcgtacaa gaactacgac acgctcgaga aggacgagaa cgcccgcgcc tggctgtatc
     7681 agatcgcgca ccgggtcgcc ctcgacgagc tccgtcgccg caagatcatc cggatgatcc
     7741 cgtggaccgg cgagtcccac ggcgcggcac cgtccgccga gcgcatcgcg atggacctgc
     7801 gcctctccgg ccccctcgag cgggccctcg cctccatccc cgagcgccag cgtgccgcgc
     7861 tgctcctcgc cgagctccat gacctcaccg gcgtcgagct cgccgcggcg atgggcgtca
     7921 gccatgtcgc ggcacgcgcg atcctgaccc gcgctcgcga gagcctccgg cgcgccctcg
     7981 ccgccgagaa ggacgccgag cgcgccgccg aggcagaccg tgacgcgcgc ttcgcagcgg
     8041 gggccggtag cgaccgatga ccctgccgtt ccgccgccgc caccatgacg atgagggcac
     8101 gcacgaccgc gcccgcgccc tgacctcgcg cgagatgatc gagtcactgc ccgaggaaga
     8161 gacggcgtgg ctgacccgcc acctcgccga gtgcggtgag tgcacccagg accgcgaggc
     8221 gtatctcgcc gatcgggcgc tgctccggag ccttcgcgac aacacacccg aaccaccgcg
     8281 cgacctgtgg gctcgaacct ctgccgcgct cgatcgcgag gcccgcagcc accgcggcac
     8341 cggcgcccgc gctcgccgag acgcccgagc gggtcgccgc cagctcgggc ggccctacgg
     8401 tgcggcagcg ggcatgctcg tggtgctcat cgtggtcgcg ttggccagca tcaagccgat
     8461 cgtgccccca agcgcgacgc ccggcgcctc gaatgtggcg gtcgcaacac cgccggccgc
     8521 cgagccgacg cagatcatcg tgaccgccgg acgtgtcggc tggatccggg ccgcgaacga
     8581 cgggcagtgg gagctgatcc tcgccgacgt caaggccgtc tgcccgcgca gccggcccaa
     8641 ctgcggccag ctggtcgagg atgatccggg ccggaccatc aacctgggca gcgccccgac
     8701 cggcggcttc acgatctcgg agaacgagca gcagctggtc atcgagcgca atgccgaggg
     8761 cgcggcgccg ggtcggatcc tcgtggtccc gatcccgccc gcgagccccg cggtgacacc
     8821 cgccccggtc gataccccga agccggtcga taccccgaag ccgatagaca cgccgaagcc
     8881 aattgatacg ccgacgccaa tcgacacccc gagtcctgcc gacacgccga ccccggcgcc
     8941 gcccacgggc gaaccggttt cgccggcggt gccgactccc gtgccaacgt cgacgacgcc
     9001 cgacggcacg atcgagatcg catccggcgt gaccatggtc ggcgaggcgg cgtactcgag
     9061 cgactccaag tggctggcct tttccgcgcg tccgagcgac ggct
//
LOCUS       DATBSW010000257         4882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1082352, whole genome shotgun sequence.
ACCESSION   DATBSW010000257 DATBSW010000000
VERSION     DATBSW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082352"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /gene="fumC"
                     /locus_tag="VIF63_07245"
     CDS             <1..228
                     /gene="fumC"
                     /locus_tag="VIF63_07245"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="class II family (does not require metal);
                     tetrameric enzyme; fumarase C; reversibly converts
                     (S)-malate to fumarate and water; functions in the TCA
                     cycle; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY8239215.1"
                     /translation="GLEADLERIDEHVANSLMLVTALTPRIGYDKAAEIAKKAHHEGT
                     TLRVAALELGYLDANEFDALMRPEAMTRPSA"
     gene            246..1139
                     /locus_tag="VIF63_07250"
     CDS             246..1139
                     /locus_tag="VIF63_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY8239216.1"
                     /translation="MRWRPSSRNQVEDRRGAGGGLGGVSMGGLGGGGFPIPMGRGGLS
                     LGGIVLVIIIYVVIQLLSGSGAPTPQGGVTGSLDPNDDIGIFINAVTVDIQDFWEGEF
                     QAAGKQYPATVTVLFTSQTQTGCGVASSATGPFYCPVDEKVFLDQSFFQELADRFGAP
                     GDFAQAYVIAHEYGHRVQDVLGIMDSVQSQQQSSPSKANDLSIRLELQADCLAGVWAH
                     SIATDPDESEVESITEADVKEALTAAAAVGDDRIQEQATGTIDKESWTHGSADQRTKW
                     FNTGFKVGTTDSCDTFAVATP"
     gene            complement(1155..1520)
                     /locus_tag="VIF63_07255"
     CDS             complement(1155..1520)
                     /locus_tag="VIF63_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY8239217.1"
                     /translation="MPDMPKFEKPAPELVARFEAVLKRVGTPETTRRPMFGHPCAWVG
                     GNMASGLFAQTWWVRLPPDRLASVLASGKGRPFQVMPGRDMKGYVALPDEDVADDARI
                     DAWVSEALDYTATLPPKKK"
     gene            complement(1544..2479)
                     /locus_tag="VIF63_07260"
     CDS             complement(1544..2479)
                     /locus_tag="VIF63_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239218.1"
                     /translation="MAPIDLRSVLGPLARGSGDPTMRVAAGSAIRASATPDGPGTIEI
                     RADGTAIRARAWGPGAGWLLDHLPAALGLDDDDAGFDPSLHPVVAGLAHRLGRPRLGR
                     TGAVWEALLPAMLEQRITGTEAWRNYRRLVKAHGEPAPGPHGLLLPPTPATVARLPSW
                     TTTGLGIEPRRGALLRRIAREASRLEALGAAARESGRGGDGARALEAALRAHQGIGPW
                     TAAEVTLRALGDPDALSVGDAHLSNVVAFALTGAPRGTDERMVELLAPWTGHRARVVR
                     LLESARIAPPRYGPRVTPRDLTRDFVRPQRATRRA"
     gene            2617..3213
                     /locus_tag="VIF63_07265"
     CDS             2617..3213
                     /locus_tag="VIF63_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY8239219.1"
                     /translation="MRLPRVVARAALIAALASVVVPGLVGSRIPSAPAAINPTAFQEV
                     ALDRSEASMTTPQLDPDTASAGQLDANAILSEPRLAPGATFGPRPNVALPNAKTLVKI
                     LWHLDSNISWYGPGFYGNGGACGMMPGGMTKDTVGVAHRTLPCGTSVTFRYNGRTVTT
                     RVIDRGPYVSGRTWDMTHGLCALLNHCFTGGGVYYRIN"
     gene            complement(3230..>4882)
                     /locus_tag="VIF63_07270"
     CDS             complement(3230..>4882)
                     /locus_tag="VIF63_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8239220.1"
                     /translation="LAGLTSVASAPNAASGDSTSVTRLLVLVATVGYLGAFVPPGWFR
                     RFINRAASFQLVRNLVAPTTEMSPGSLWADLATTARDILGASRVTIQSSAPGAPLAVA
                     SSIWAGDAEGRPKVISSKVELVVRPGQELSERLIADVEGRQLFVDDDLALIADLAAMT
                     AHTIDREEMLIGLGEARREAAESRSIRASEARFRALLDADPNAILALDEESRVTWATR
                     QAGELFGCEVDRLIGMALGDLVALHKDETPTTGGDRPIFRSETTGRRVDGTHFPAEIA
                     RSTFELDGRPFQVAVLSDVTWRHEADQIRDRFLGVLSHELRTPVTSIYGGTQLLLGRG
                     ARLDPETRNELLVNVAGEAERLQRMIENLVAMARIERGGDFGGTRPVLIDRIIKQLVE
                     RERGLWPEVTIKFVANGPVQMVAADEEYLAQIMRNLLSNAAKYSGAGSTVEVSLEDGE
                     GEVIIRVRDDGPGIADEDAERLFGLYYRSARNASIAPGAGIGLFVCRELVATMGGRIW
                     AKSLPDKGAEFGFSIPAYVDEMDALPFVPTPTERQDKASTPV"
BASE COUNT          731 a         1727 c         1724 g          700 t
ORIGIN      
        1 ggcctcgagg cagatctcga gcggatcgac gagcatgtcg cgaactcgct catgttggtg
       61 acggccctga cacctcggat cgggtatgac aaggctgccg aaatcgccaa gaaggcgcac
      121 cacgagggca cgacgttgcg cgtcgccgct ctcgagctcg gctatctcga cgcgaacgag
      181 ttcgacgcgc tcatgcgacc ggaggcgatg acccgccctt ccgcctgagc tggaaggagc
      241 actgcatgcg ctggagacca tcgagcagga accaggtcga ggaccgccgc ggagccggcg
      301 gcgggctcgg cggcgtcagc atgggcggcc tcggcggcgg cggcttcccg atcccgatgg
      361 gccggggcgg actgagcctc ggcggcatcg tcctcgtcat catcatctac gtcgtcatcc
      421 agctgctcag cggcagcggc gcgccgacac cgcagggtgg cgtcaccggg tccctggacc
      481 cgaacgacga catcggcatc ttcatcaacg cggtgaccgt cgacatccag gacttctggg
      541 agggcgagtt ccaggccgcc ggcaagcagt acccggcgac cgtcaccgtc ctcttcacga
      601 gccagaccca gaccggctgc ggcgtggcct cgtcggcgac cggcccgttc tattgcccgg
      661 tcgacgagaa ggtcttcctc gaccagtcct tcttccagga gctcgccgac cgcttcgggg
      721 cccccggcga cttcgcccag gcgtacgtca tcgcccacga gtacggacat cgcgtgcagg
      781 acgtgctcgg gatcatggat tcggtccagt cccagcagca atcgagcccg tcgaaagcga
      841 acgacctgtc gatccggctc gagctccagg ccgactgcct cgccggcgtg tgggcgcatt
      901 cgatcgccac cgatcccgac gagtccgagg tcgaatcgat caccgaggcg gacgtcaagg
      961 aggccctgac cgccgctgcg gcggtcggcg acgatcggat ccaggagcag gcaacgggca
     1021 cgatcgacaa ggaaagctgg acccacggct ccgccgacca gcggaccaag tggttcaaca
     1081 cgggcttcaa ggtcggcacg accgacagct gcgacacctt cgccgtggca acgccttagc
     1141 ccggggccgc cgggctattt cttcttgggc ggcagggtcg cggtgtagtc gagggcctcg
     1201 ctgacccatg cgtcgatgcg ggcgtcgtcc gcgacgtcct cgtcgggcag cgccacgtag
     1261 cccttcatgt cccgaccggg catgacctgg aacggccggc ccttgccgga ggcgagcacg
     1321 gaggcgagac ggtcgggcgg gagccggacc caccacgtct gcgcgaagag gccactggcc
     1381 atgttgccgc cgacccacgc gcatgggtgg ccgaacatcg gccggcgcgt ggtctcgggc
     1441 gtgccgacgc gtttgagcac cgcttcgaac ctggcgacga gctccggcgc gggcttttcg
     1501 aacttcggca tgtcgggcat gcgctgacga tacccctcgg gcgtcaggcg cggcgcgtgg
     1561 cccgctgggg gcgcacgaag tcgcgggtca gatcgcgcgg cgtgacgcgc gggccgtagc
     1621 ggggcggcgc gatccgcgcg gactcgagca ggcgcacgac ccgggcgcga tggcccgtcc
     1681 acggcgcaag gagctcgacc atccgctcgt ccgtgccgcg aggcgcgccc gtgagcgcga
     1741 acgccacgac attcgacagg tgggcatcgc cgacgctcag cgcgtccggg tcgccgaggg
     1801 cgcggagcgt cacctcggcc gccgtccatg gcccgatccc ctggtgggct cgcagtgccg
     1861 cctcgagcgc ccgggctccg tcgccgccac ggcccgattc gcgcgcggcg gcgcccaacg
     1921 cctccagccg gctcgcctcc cgcgcgattc gccgcaggag cgcaccgcgc cgcggctcga
     1981 tcccgaggcc ggtggtcgtc caggacggga gtcgtgcaac ggtcgccggc gtcgggggca
     2041 ggagcagccc atgtggacca ggcgccggct caccgtgggc cttcaccagc cggcgatagt
     2101 tgcgccacgc ctccgtgccg gtgatgcgct gctctagcat cgccggcagg agcgcctccc
     2161 acacggcgcc ggtccggccg aggcgcgggc gacccagtcg atgggcgagg ccggcgacga
     2221 ccggatggag gctcggatcg aagcccgcgt cgtcgtcgtc gagcccgagc gccgccggca
     2281 ggtggtcgag cagccagccg gcgcctgggc cccacgcgcg ggctcggatc gcggttccgt
     2341 cggcgcggat ctcgatcgtg ccggggccat ccggcgtcgc ggaggcgcga atggccgatc
     2401 ctgccgcgac gcgcatggtc gggtcgccgg agccccgcgc gagcggcccc aggaccgagc
     2461 gcagatcgat cggggccacc agcgggatcg ttcgcggcgc acctccgtcg tgctcgccgg
     2521 tgccgtccat cagcgagctg caacggtgct gcgagccgtc ggatgaggtg gcagccgaca
     2581 cgctgcaagc catcctgggg tacagtccac ccagacgtgc gactccccag agtcgtcgcg
     2641 cgcgctgccc tcattgcggc gctcgcctcg gtcgtcgtcc ccgggcttgt tggttcgcgc
     2701 atcccgagcg cacccgcagc catcaatcca acggcctttc aagaggtcgc actggatcgt
     2761 tcggaggcgt cgatgacgac cccgcaactc gatcctgaca ccgcgtccgc aggccaactc
     2821 gatgccaatg cgatcctcag cgaaccgcgc cttgcgccgg gcgcgacgtt cggcccgcgg
     2881 cccaacgttg ccctgccgaa cgccaagacg ctggtgaaga tcctctggca cctcgacagc
     2941 aacatcagct ggtacggccc ggggttctac ggcaacggcg gcgcgtgcgg gatgatgccg
     3001 ggtggcatga ccaaggacac ggtcggcgtc gcgcaccgga ccctgccgtg cggcacgagc
     3061 gtgaccttcc ggtacaacgg ccgcaccgtc acgacgcggg tgatcgatcg cgggccgtac
     3121 gtatccggcc ggacctggga catgacccac gggctgtgcg cgctcctgaa ccactgcttc
     3181 acgggcggag gcgtgtacta ccgcatcaac tgatgatgcg gcgtcgcgtt cagacgggcg
     3241 tgctcgcctt gtcctgacgc tcggtggggg tcgggacgaa cgggagcgcg tccatctcgt
     3301 caacgtaggc cgggatgctg aagccgaact ccgcgccctt gtcgggcagc gacttcgccc
     3361 agatgcgtcc gcccatcgtc gccacgagct cgcggcacac gaacagcccg atgcccgcgc
     3421 ccggtgcgat cgacgcgttg cgcgcagatc ggtagtacag gccgaacagg cgctcggcgt
     3481 cctcgtcggc gatgcccggg ccgtcgtccc ggacgcggat gatgacctcg ccctcgccgt
     3541 cctcgaggct gacctcgacc gtcgacccgg cgccgctgta cttcgccgcg ttcgacagca
     3601 ggttgcgcat gatctgggcg aggtactcct cgtcggccgc gaccatctgg accggcccgt
     3661 tcgcgacgaa cttgatcgtg acctcaggcc atagcccgcg ctcacgctcg acgagctgct
     3721 tgatgatccg gtcgatgagc acgggcctgg tgccaccgaa gtcgccgccg cgctcgatgc
     3781 gggccatcgc gacgaggttc tcgatcatcc gctggagccg ctcggcctcg ccggccacat
     3841 tcacgagaag ctcgttgcgc gtctcggggt cgaggcgcgc cccgcgcccg agcagcagct
     3901 gcgtcccgcc gtagatcgat gtcaccggcg tccggagctc gtgcgacagg acgccgagga
     3961 accgatcgcg gatctggtcg gcttcgtggc gccacgtgac atccgagagc acggcgacct
     4021 ggaacggccg gccgtcgagc tcgaaggtgc tgcgagcgat ctcggcgggg aagtgggtgc
     4081 cgtccacgcg ccggcccgtg gtctcggagc ggaagatcgg tcggtcgccg ccggtcgtcg
     4141 gcgtctcgtc cttgtgcagc gcgacgaggt cgccgagggc catgccgatg agccgatcca
     4201 cctcgcagcc gaacagctcg cccgcctggc gcgtcgccca ggtgacgcga ctctcctcat
     4261 cgagggcgag gatcgcgttc gggtccgcat cgagcagggc gcggaagcgc gcttcgctgg
     4321 cccggatgga gcgggattcc gccgcctcgc ggcgcgcctc gccgaggccg atgagcatct
     4381 cctcacgatc gatcgtgtgg gcggtcatcg cagcgaggtc ggcgatcagc gcgaggtcgt
     4441 cgtcgacgaa gagctggcga ccttcgacat ccgcgatgag gcgttcggac agctcctggc
     4501 cgggacgcac gacaagctcc accttggagc tgatcacctt cggccggcct tcggcgtcgc
     4561 cagcccagat gctgctggcg accgcgagcg gcgcccccgg cgcgctggac tggatcgtga
     4621 cccggctggc cccgaggatg tcacgtgcgg tggtcgcgag gtcggcccac aggctgcccg
     4681 ggctcatttc ggtcgtgggg gcgacgaggt tcctgacgag ctggaacgat gccgcacggt
     4741 tgatgaaccg acggaaccag ccgggcggaa cgaaggcgcc gaggtagccg acggtcgcga
     4801 cgagcacgag cagccgcgtg acggacgtcg aatcgcccga ggccgcgttc ggcgcgctcg
     4861 cgaccgacgt gaggcccgcg ag
//
LOCUS       DATBSW010000258         8948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_730177, whole genome shotgun sequence.
ACCESSION   DATBSW010000258 DATBSW010000000
VERSION     DATBSW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730177"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1655
                     /locus_tag="VIF63_07275"
     CDS             114..1655
                     /locus_tag="VIF63_07275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8239221.1"
                     /translation="MAESRSEVVQVTPVALGPRVAELIERGGRFVMLVGLDERSVGGA
                     GLAVEAVVSTAEGGGSGIGGAGLTRLRAQLPLGEAAYPSISLQVPAAHWDEREAKDLL
                     GIVPLGHPDPRRLVLHERYPAGYAPLRKDVPADVRPPLADRHFVPFEVHGEGVYQLPV
                     GPIHAGVIEPGHFRFSAIGERVLHLDARLFFVHRGLEKLVEGRSFGAALPIVERACGV
                     CTVTHAMAYAHAVEAVTRTAIPDRAAWARTLLAELERLYNHVGDLGNICAGVGFQAGT
                     SRFGWLKERALRLNDALTGHRYLMGVVAPGGLVADLHPDGLTILSAEIDDIAAELASS
                     IRAVVRSEGVLDRFHGTGVVPADVAASLGALGVAARASGISMDLRRDRPYAAYRELDV
                     SPATAHGGDVAARFHVRAQEAHECLRLIRESARRMPAGEIWAPPAVEPVVGASAVTGV
                     EGPRGASWIWLRTGRGGTVDRLRLRSASFANWPVVAAAAPGNLVPDFPLINKSFELCY
                     ACTDR"
     gene            1657..2001
                     /locus_tag="VIF63_07280"
     CDS             1657..2001
                     /locus_tag="VIF63_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8239222.1"
                     /translation="MARDVFDRILRPLRRGPVTSRYPDVRPELPQAARGLPELDPGRC
                     DSSGTCAEVCPTRAIAVSDGRWTLDAGACIFCGACARACPRDAIRLGHRIELAVVEVR
                     DLKTERAVRGPS"
     gene            1998..2492
                     /locus_tag="VIF63_07285"
     CDS             1998..2492
                     /locus_tag="VIF63_07285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY8239223.1"
                     /translation="MSGVSVDVIPAGELDVRPRLRQTIRRVLGRSLHIRHLDAGSCNG
                     CDWEIAALLNPYHDVQRLGIDFVASPRHADLLLVTGIMTRNLEAAALRTYEAMPDPRL
                     VVATGACAISGGVFVGTYAGRDGVGDVLPVDVMIPGCPPRPEALIHGLLVAVGRLEAF
                     DPTS"
     gene            complement(2502..3293)
                     /gene="radC"
                     /locus_tag="VIF63_07290"
     CDS             complement(2502..3293)
                     /gene="radC"
                     /locus_tag="VIF63_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY8239224.1"
                     /translation="MRPREQPIPFDLAVARGRGAAARAAGGHSIHELPAAERPRERLH
                     LRGAAGLTAAELIGLLWGSGTAGRSAIDVATDAITRFDGLSGLARASGPELEAIPGIG
                     EAKAAQLQAAFELGRRLLADWPAGRWSIRSPRDVADRLVLQMGRLEREELRVVILNTK
                     NAVLRVVTAYQGNVSASLVRVGELFRDAVRLDAAGVILVHNHPSGDPTPSPDDLHLTA
                     EALAAGRLLDIDVLDHLVIGHDAYVSMRDRGIAFDRIGSRPPPAA"
     gene            complement(3516..4520)
                     /locus_tag="VIF63_07295"
     CDS             complement(3516..4520)
                     /locus_tag="VIF63_07295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY8239225.1"
                     /translation="MTEPSEPSDPAGPAEPPPPRSFGLTTFTIEGRQAPALFVVGWLA
                     ALIGLGFAGLTLLGITGLAGAILWIVGLAIGSVGLILLGGSQTIERRAAGAAYIGPSP
                     SIVFLTVLAGSQLAGFAVGLPLSLIGAAIPRPVGDLIGGIVQAAVFVGVLRVMVVAAG
                     SMRWSEMGLVARSRDAVLGLVRGTIYVVPVIALTAIVAAIAGQLAGVVPESPLPPTGT
                     TSGLLLHLLAGAVIAPVSEELLFRGFALTAWRRRFGARGAIIRSSIVFVLAHVLFVGG
                     DGFREAAALAFVAATARLPVALVLGWLYVRTGSLWAPIGLHATYNAVLIILAEAVLAR
                     "
     gene            complement(4517..5143)
                     /locus_tag="VIF63_07300"
     CDS             complement(4517..5143)
                     /locus_tag="VIF63_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8239226.1"
                     /translation="MDEIFTAIGDAIGSIFDNPVIKLGIQLAVIYWIILWLAASYWAF
                     RDMQGRSDNPLAPYLAAAFVIVFTPILFPLAVFTYKVVRPQERIGEVYERNLAEEAML
                     GEIEAIPHCPNCDRRVESAWIICPTCRTRLNRVCPNCARLVGLDWTLCAWCGREFERH
                     DQLAASTASPIVSLPTLEALPSGELEPVVERKRPAVRAPRPSDQAADG"
     gene            complement(5276..6214)
                     /locus_tag="VIF63_07305"
     CDS             complement(5276..6214)
                     /locus_tag="VIF63_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239227.1"
                     /translation="MASAFVATLANPTWWAIALAAFLVRGGLLLILLPLITLPTTASL
                     TTDFAPVLETLLLGRSSLAGLAMMLALAGGIVAAVAAAGLAGSWLDLALVREAAADED
                     VDLPWRPVNGSIREAFSIRLTAHLPTLAALAYAAVRLVTVGYEEFTSPGDSGMPVALR
                     IISRAPEALVLVVLAWLLGETVGSLAARRAAIGLGRSAALRESVGQVLRPRGLATLAI
                     TSAALIGVWLPFEGTVRLAWEHIRAYLLDSAPSVPLAAALVLLISTYVLGLAVVGAAL
                     AWRATAWTAEVAPPPVLDRGLPLGPAEAAGPEIARG"
     gene            complement(6267..6641)
                     /locus_tag="VIF63_07310"
     CDS             complement(6267..6641)
                     /locus_tag="VIF63_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239228.1"
                     /translation="MSQPSAPADDWWTALLWIADADGLATFVELAPAAGPPPEPPLAR
                     LGPSLAGGLSGMILEDAGRLQIRLGLAIPPTDPERPWRCPAVVRAAFRWEPARAASMR
                     PNAISSEVLTAFRRAIEGLSRR"
     gene            complement(6746..7171)
                     /locus_tag="VIF63_07315"
     CDS             complement(6746..7171)
                     /locus_tag="VIF63_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239229.1"
                     /translation="MSPRKTEASGETATCPWCSATVPVAAVTCPSCGASLRDAADGEV
                     LGVTQVDPSAASRAKRIKTGNFASFIGASDGPDGPTVGKVEPPTDEVRQEMLRLELAA
                     IDAEIEAKRKSAEAEAAVEAAELAPEAAKSAPEDEATSG"
     gene            complement(7191..8906)
                     /gene="guaA"
                     /locus_tag="VIF63_07320"
     CDS             complement(7191..8906)
                     /gene="guaA"
                     /locus_tag="VIF63_07320"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY8239230.1"
                     /translation="MSGKGKTVHGEAANAGHRVLAPDDAEVEAYLETALNAPGPDRAV
                     GDRPAGGSDGVVVVLDFGSQFAQLIARRVRELNVYSELLPHDTPLAEIERRGASAIIL
                     SGGPNSVYDVDAPKPDPLIWAGKLPVLGICYGAQLMASELGGHVQSTQKREYGPATVT
                     IGSEDGLFAGLDRVQPVWMSHGDSITELPEGFTGTAQTDSTAFAGLADHSRRLYGIQF
                     HPEVAHTPHGKDVLRNFVVGLAGVIPNWTPAHFIDSTVAEIRRRVDEHASAVGSDGKV
                     ICALSGGVDSAVAAALVHRAVGDRLTCIYVDHGLMRKRESELLRATFAENLGMNLVMV
                     DARTRFLARLAGVIDPEQKRRIIGDEFIRVFEEEAEKLGQIDFLTQGTLYHDVIESAT
                     SETKAAQKIKTHHNVGGLPADLRFKLIEPLRYLFKDEVRAVGLELGLPEQMVQRQPFP
                     GPGLAIRIIGEVTATRLDTLREADWIVIDEIKAAGLYRNVWQSFAILTPINSVGVMGD
                     GRTYANVVAIRAVTSEDGMTADWAKLPYEVLGKISSRIVNEVPGVNRVVYDISSKPPA
                     TIEWE"
BASE COUNT         1339 a         3123 c         3239 g         1247 t
ORIGIN      
        1 cggcgccatg gcgctcgccg tgccgctcgc gctggtcgtc gtcctcggcg tatggacgcc
       61 aacaccgctg gtgaacgttc tcgagctggt cgcgggcatc ctcgggggcg gtcgtggctg
      121 agtcgaggag cgaggtggtc caggtgacgc cggtggcact cgggccgcgg gtcgccgagc
      181 tgatcgagcg cggaggcagg ttcgtgatgc tcgtagggct cgacgagcgg tcggtcgggg
      241 gcgcaggtct ggccgtggag gctgtggtca gcactgccga aggcggcgga tccgggatcg
      301 gcggagccgg cctgacgaga ctccgggcgc agctgccgct cggcgaggcg gcgtacccgt
      361 cgatcagcct gcaggtgccg gccgcgcact gggacgaacg tgaggcgaag gacctgctcg
      421 gcattgtccc gctcggccat cccgacccgc gccggctcgt cctgcacgag cggtatccgg
      481 ccgggtatgc gccgctccgt aaagatgtcc cggcggacgt ccggccaccg ctggcagacc
      541 gccatttcgt acccttcgag gttcacgggg agggcgtcta ccagttgccg gtcggaccga
      601 tccacgccgg cgtcatcgag ccggggcatt tccggttctc ggcgatcggc gagcgcgtcc
      661 tgcacctcga cgcgcgcctg ttcttcgtgc atcgggggct cgagaagctc gtcgagggtc
      721 gctcgttcgg agccgccctg ccgatcgtcg agcgggcctg tggcgtgtgc acggtcaccc
      781 acgccatggc ctacgcccat gcggtcgagg ccgttacccg gacggcaatt ccggaccggg
      841 ctgcctgggc acggacactg ctcgccgagc tcgaacggct ctacaaccac gtcggcgatc
      901 tcggcaacat ctgcgccggg gtgggcttcc aggcggggac ctcgcgattc ggctggctca
      961 aggagcgcgc gctgcggctc aacgatgcgc tgaccggtca ccgctacctg atgggcgtgg
     1021 tcgcgcccgg gggcctggta gcggacctgc acccggacgg cctcacgatc ctgtcggcgg
     1081 agatcgatga catcgcggcg gagctcgcct catcgatccg tgccgtcgtc aggtccgagg
     1141 gcgtcctcga ccgcttccac ggcacgggcg tcgtgcccgc ggacgtggct gcatccctag
     1201 gggctctcgg tgtggcggca cgagcgagcg gcatttccat ggacctgcgt cgtgaccgac
     1261 cgtacgccgc gtaccgcgaa ctcgacgttt cgcccgctac cgcccacggc ggcgacgttg
     1321 cggcccgttt ccatgtcagg gcgcaggagg cacacgagtg cctgcgactg atccgcgaat
     1381 cggcgcggag gatgccggcc ggggagatct gggcgccgcc ggcggtcgaa ccggtggtgg
     1441 gcgcgtctgc cgtcaccggc gtcgaaggcc cgcgcggggc gtcctggatc tggcttcgca
     1501 ccggccgtgg cgggacggtg gatcgcctgc ggctgcgatc tgcgtcgttc gcgaactggc
     1561 ccgtggtcgc ggccgcggcc ccgggcaacc tcgtgccgga cttccccttg atcaacaaga
     1621 gcttcgagct ctgttacgcc tgtacggatc gctgagatgg cacgcgacgt cttcgacaga
     1681 attcttcgac cgctgcgccg cggccccgtc acgagccgct atcccgatgt ccgaccggag
     1741 ctgccgcagg ctgcacgggg cctgcccgag ctcgatcccg gccggtgtga cagcagcggt
     1801 acgtgcgccg aggtctgccc gacgcgggcg atcgccgtca gcgacgggcg gtggacactg
     1861 gatgccggcg cctgcatctt ctgcggtgcc tgcgcccgtg cctgtccgcg cgacgccatc
     1921 cgcctcggcc atcggatcga gctcgccgtc gtcgaggtcc gcgacctgaa gaccgagcgg
     1981 gccgtccgag gcccgtcatg agcggggtgt cggtcgacgt gatcccggca ggcgagctcg
     2041 acgttcggcc gcgtttgcga cagacgatcc ggcgcgtgct cggccgatcg ctgcacatcc
     2101 gccaccttga tgccggctcg tgcaatggct gcgactggga gatcgctgcc ctcctcaatc
     2161 cgtaccacga cgtccagcgc ctgggcattg acttcgtcgc gtcgccgcgc cacgccgacc
     2221 tcctcctcgt gaccgggatc atgacccgaa atctcgaggc ggcggccctt cggacctacg
     2281 aggccatgcc cgatccacgc ctcgtcgtcg cgaccggcgc ctgcgccatc agcggcggcg
     2341 ttttcgtcgg gacctacgcc ggtcgcgatg gggtcggcga cgtgctgccc gttgacgtga
     2401 tgatcccggg ctgcccaccg cgccccgagg cgctgatcca tgggctcctc gttgccgtcg
     2461 gccgcctgga ggcattcgac ccgacgagct gacgcgcggc ttcaggcggc cggcggcggc
     2521 cggctcccga tccggtcgaa tgcgatgccg cggtcgcgca tcgagacgta ggcatcgtgg
     2581 ccgatcacga ggtggtcgag cacgtcgatg tcgaggagac ggccggctgc cagggcctct
     2641 gcggtcaggt gcaggtcgtc cggtgacggc gtcgggtcgc ccgacggatg gttgtggacg
     2701 aggatcacgc ccgcggcatc gaggcgaacc gcatcgcgga acagctcgcc gacccgaacg
     2761 agcgaggcgc tgacgttgcc ctggtaggcg gtcacgacgc ggagcacggc gttcttcgtg
     2821 ttcaggatca ccacgcgcag ctcctcgcgc tcgaggcggc ccatctggag cacgagccgg
     2881 tccgcgacgt cgcgcggcga gcggatcgac cagcgcccag ccggccaatc ggccagaagc
     2941 cgacgaccga gctcgaacgc tgcctggagc tgggccgcct tcgcctcgcc gatgccgggg
     3001 atggcctcga gctcgggccc gctggcccgg gcgaggccgc tcaggccgtc gaatcgggtg
     3061 atcgcgtcgg tggcgacatc gatcgcggat cggccggccg tgccggagcc ccacagcagc
     3121 ccgatgagct ccgctgcggt cagcccggcc gcgccgcgca ggtggaggcg ctcccgcggg
     3181 cgctcggccg cgggcagctc gtggatggag tgtccgccgg ccgcgcgggc ggcggcgcca
     3241 cggccacgag cgaccgcgag atcgaacggg atcggctgtt cgcgggggcg catgggagaa
     3301 cctctcgtgc ggcgagcggc gtcatggacg actggccgcg actgcgttgt ctctcgcagg
     3361 gcgacgacgc cgccgccgcg acgagcggcg ccctcgagcc cggacgacgg aagcccgccg
     3421 attccccccg ccatccggac ccgaggacac gacggaggct acgcggcgac gctcacccgg
     3481 cggtcacccg ggtctcaccc gggaattacc gggaatcacc gggccaggac ggcctcggcg
     3541 aggatgatca ggacggcgtt gtacgtcgcg tggagcccga tcggcgccca cagcgagccg
     3601 gtgcgcacgt acagccagcc gagcacgagc gcgaccggca gccgcgccgt cgctgccacg
     3661 aaggccagcg cggcggcctc tcgaaaaccg tcgccaccga cgaacagcac gtgggcgagc
     3721 acgaacacga tcgacgaccg gatgatcgcg ccgcgcgcac cgaaccttcg acgccaggcg
     3781 gtcagtgcga acccccggaa caacagctcc tcggaaacgg gcgcgatgac ggcaccggcg
     3841 agcaggtgca ggagcaggcc gctcgtcgtg ccggtgggcg gcagcgggct ctcgggaacg
     3901 accccggcga gctgcccggc gatcgcggcg acgattgcgg tcaacgcgat gacggggacg
     3961 acgtagatcg tgccccgaac gagaccaagg accgcatccc gcgatctggc gacgaggccc
     4021 atctcgctcc agcgcatcga gccggcggcg acgaccatca cccgcaagac gccgacgaac
     4081 acggcggcct gcacgatccc gccgatgagg tccccaaccg gtcgaggaat ggccgcgccg
     4141 atcagcgaca gcggcagtcc gaccgcgaag cccgcgagct gcgagccggc gaggaccgtg
     4201 aggaagacga tcgagggtga cggcccgatg taggcggcgc ccgccgcccg gcgttcgatg
     4261 gtctgcgagc cacccagcag gatgagcccg accgacccga tggcgaggcc gacgatccac
     4321 aggatcgcgc cggccaggcc ggtgatcccg agcagcgtga ggccggcgaa gccgagcccg
     4381 atgagcgcgg ccagccagcc gacgacgaag agcgccggcg cctgccggcc ctcgatggtg
     4441 aaggtcgtca ggccgaacga gcgcggcggg ggtggctcag cgggcccggc gggatcggag
     4501 ggctcggaag gctccgtcaa ccgtccgcgg cttgatcgga cgggcgaggc gcacggacgg
     4561 cgggccgttt tcgctcgacg accggctcga gctcacccga gggcagcgcc tcgagggtcg
     4621 gcaggctcac gatcggtgac gcggtgctcg ctgcgagctg gtcgtggcgc tcgaactcgc
     4681 gcccgcacca cgcgcacagc gtccagtcga gtccgaccag gcgggcgcag ttcgggcaga
     4741 cgcgattcag ccgggtccgg cacgtcggac agatgatcca ggccgactcg actcgccgat
     4801 cgcagttggg gcaatgcggg atcgcctcga tctcgccgag catcgcctcc tcggccaggt
     4861 tccgctcgta cacctcgccg atgcgctcct gcggccggac gaccttgtac gtgaacacgg
     4921 cgagcgggaa caggatcggc gtgaagacga tgacgaaggc ggccgcgagg tacggcgcga
     4981 gcgggttgtc acttcggccc tgcatgtcgc ggaaggccca gtacgacgcc gcgagccaca
     5041 ggatgatcca gtagatgacg gcgagctgga tgccgagctt gatgaccgga ttgtcgaaga
     5101 tcgagccgat cgcatcgccg atggcggtga agatctcatc catgcggcga gccggttcct
     5161 ccgtcctgaa caccgtcgcc gcgggtggac cgcagcgacc ggcgcgcagt gtaacagggg
     5221 cacttttggc ccccgactga ccctgtggac gcccgaacga cgggttgcgt gacgatcagc
     5281 ctcgggcgat ttccgggccg gcggcttcgg ccggcccgag cggcagccca cggtcgagca
     5341 cgggcggcgg cgcaacctcg gccgtccagg cggtcgcccg ccacgcgagc gcggctccga
     5401 ccacggccag gccgaggaca taggtggaga tcaacagcac cagcgccgcc gcgagcggca
     5461 ccgatggcgc actgtcgagc aggtacgccc ggatgtgctc ccacgccagg cggacggtgc
     5521 cctcgaacgg cagccatacg ccgatcagtg ccgccgacgt gatcgcgagc gtcgccaggc
     5581 cgcgcggccg cagcacctgg ccgaccgatt cgcgcagcgc cgccgacctt ccgagcccga
     5641 tggcggcgcg acgcgccgcg agcgacccga cggtctcgcc gagcagccac gcgagcacga
     5701 ccaggacgag ggcctccggg gcgcgcgaga tgatccggag cgcaacgggc atgcccgagt
     5761 caccgggcga ggtgaactcc tcgtagccga cggtgaccag gcgcacggcc gcgtaggcca
     5821 gcgccgccag cgtcggcagg tgcgcggtga gccggatgct gaaggcctcc cggatcgagc
     5881 cgttgaccgg ccgccaggga aggtcgacgt cctcgtccgc ggcggcctca cggaccagcg
     5941 ccaggtccag ccaggagccg gcaagcccgg cagcggcgac ggcggcgacg atcccgcctg
     6001 ccagcgccag catcatcgcg aggccggcca gcgaggaccg gccaagcagc agcgtctcga
     6061 gcacgggcgc gaaatccgtg gtcagcgagg cagtcgtcgg cagggtgatc aggggcagga
     6121 ggatcaacag cagcccaccg cgaacaagga aggccgcgag ggcgatcgcc caccacgtcg
     6181 ggttcgcgag cgtcgcgacg aaggcggacg ccatcgatcc cgcccagctc ctgcccatca
     6241 gttcgaggat agcccgctgc cactgactac cttcgcgaga gtccctcgat cgcccgccgg
     6301 aacgcggtca ggacctcgga ggaaatggcg tttggccgca tcgaggcggc gcgcgccggc
     6361 tcccagcgaa aggcagcgcg cacgaccgcg gggcagcgcc acggccgctc cgggtccgtg
     6421 ggcgggatcg ccaggccgag acggatctgg aggcggccgg cgtcctcgag gatcatcccg
     6481 gatagcccgc cggcgagcga cggcccgagg cgggcgaggg gtggctcggg tggaggcccg
     6541 gcggccggcg cgagctccac gaacgtggcc agcccgtcgg catcggcgat ccacaggagg
     6601 gcagtccacc agtcgtcggc gggcgccgac ggttggctca tgcccgcgga ccacgatcga
     6661 tcgccgacgc ggacctcctg ctcgcgcagg aacgatggac cgggcgccgg caggcgcgcg
     6721 ccggctgctg gtggcggcgg ctggctcagc cggacgtcgc ctcgtcctcg ggcgccgact
     6781 tggcggcctc gggcgcgagc tcggcggcct cgacggcggc ctcggcctcg gccgacttgc
     6841 gcttcgcttc gatctcggcg tcgatcgccg cgagctccag gcgaagcatc tcctggcgga
     6901 cctcgtccgt cggcggttcg accttgccga cggtcgggcc gtcggggccg tcgctcgcgc
     6961 cgatgaacga cgcgaagttg ccggtcttga tccgcttcgc gcgcgacgcg gcggatggat
     7021 cgacctgggt cacgcccagg acttcgccat cggcggcatc acgcaacgac gcgccgcacg
     7081 acggacaggt gaccgccgca accggcacgg tcgcggaaca ccacgggcag gttgccgttt
     7141 cgccgctggc ttctgtcttg cgcgggctca tgaaaggacg ctcatgccac ttactcccac
     7201 tcgatcgtgg ccggtggctt cgacgagatg tcgtacacga cgcgattcac ccccggaacc
     7261 tcgttgacga ttcgactgct gatcttgccc aggacttcgt acggcagttt cgcccaatcg
     7321 gcggtcatcc cgtcttccga agtcactgcg cgaattgcta ccacgttcgc gtacgtccga
     7381 ccatccccca tcacgcccac gctgttgatg ggagtgagga ttgcgaagga ttgccacacg
     7441 ttccggtaga ggccggcggc cttgatctcg tcgatgacga tccagtccgc ctcgcgcagc
     7501 gtgtccagcc gcgtggcggt cacctcgccg atgatccgga tcgcgagccc ggggccgggg
     7561 aagggctggc gctggaccat ctgctcgggc aggccgagct ccagcccgac cgcccggacc
     7621 tcgtctttga acaggtagcg cagcggctcg atgagcttga accggagatc ggcagggagc
     7681 ccaccgacgt tgtggtgggt cttgatcttc tgggccgcct tcgtctccga ggtggcgctc
     7741 tcgatgacat cgtggtagag cgtgccctgg gtcaggaagt cgatctggcc gagcttctcg
     7801 gcctcctcct cgaagacgcg gatgaactcg tcgccgatga tccggcgctt ctgctcgggg
     7861 tcgatcacgc cggccaggcg ggcgaggaac cgcgtacggg catcgaccat caccaggttc
     7921 atgccgaggt tctcggcgaa ggttgcccgc aggagctccg attcgcgctt ccgcatcagc
     7981 ccgtggtcga cgtagatgca cgtcagccga tcacccaccg cgcgatgcac gagcgcggcc
     8041 gcgaccgccg aatcgacgcc gcccgaaagc gcgcagatga ccttcccatc cgagccgacg
     8101 gcgctcgcgt gctcgtcgac gcggcggcgg atctccgcca ccgtcgaatc gatgaagtgc
     8161 gcgggggtcc agttcggaat gacgccggcg aggcccacga cgaagtttcg gaggacgtcc
     8221 ttgccgtgcg gcgtgtgggc aacctcgggg tggaactgga tgccgtacag ccggcgggag
     8281 tggtcggcca ggccggcgaa cgcggtcgag tcggtctggg cggtgcccgt gaagccctcc
     8341 gggagctcgg tgatcgaatc gccgtggctc atccacaccg gctggacgcg gtcgaggccg
     8401 gcaaacaggc cgtcctcgct cccgatcgtg accgtcgccg ggccgtactc gcgcttctgt
     8461 gtcgactgga cgtgcccgcc gagctcgctg gccatgagct gggcgccgta gcagatcccg
     8521 agcaccggca gcttcccggc ccagatcagc ggatcgggct tcggggcgtc gacgtcgtag
     8581 acggagttcg ggccgcccga gaggatgatc gcgctggcgc cgcggcgctc gatctcggcc
     8641 aggggcgtgt cgtggggcag gagctcggag tagacgttca actcgcgcac gcggcgcgcg
     8701 atcagctggg cgaactggct cccgaaatcg aggacgacga cgacgccatc ggagccgccg
     8761 gccggtcggt caccgaccgc tcggtccggg ccaggggcat tgagcgcagt ctcgaggtac
     8821 gcctcgacct cggcgtcgtc cggggcaagg acgcggtgac ccgcgttggc ggcctcaccg
     8881 tggacggtct tgccctttcc cgacacgcgc gatccagtcc ctccgcctcg acagccgatt
     8941 ggccgtgg
//
LOCUS       DATBSW010000259        11412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1189015, whole genome shotgun sequence.
ACCESSION   DATBSW010000259 DATBSW010000000
VERSION     DATBSW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189015"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VIF63_07325"
     CDS             <1..955
                     /locus_tag="VIF63_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239231.1"
                     /translation="NPQQPAARYRRVDRRARPAEGPFATGTPPLQGKALSYNNVLDAS
                     AAGAAARLLHGPACVIVKHTNPCGAAERSTLFEAWQAALTGDPDAAFGGVVALTGLVT
                     ADVARGLTEIFLEVVVAPAFEPEALEILAAKQNLRLIEDPNLTGIPAKARADGGLTVD
                     YLGSVRSVGGAILVGAPDDSIDDPGDWLTLTSRAPTEREQADLDLAWRLARGVVSNAI
                     VLVRDRQLVGLGSGQVSRVDACRQAVVKAERFHGPVGAAGAVAASDAFFPFADGPQVL
                     LDAGVTAIVQPGGSLRDAEVLAAVDEAGAVMQITARRHFRH"
     gene            979..1416
                     /locus_tag="VIF63_07330"
     CDS             979..1416
                     /locus_tag="VIF63_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8239232.1"
                     /translation="MSKLTNEECVARYGAASAAHDVAALAALRHPEWSVYWPQSGERV
                     HTSEAFAEIIDNYPGGAPSTEVTRVVGSEDQWVVTAGNTVMLVAGSGDFWWTEWKITY
                     PDGEVYLVVDLMELRDGLVHRETVYWAAPFEAPAWRAPWVERT"
     gene            1635..3314
                     /gene="metG"
                     /locus_tag="VIF63_07335"
     CDS             1635..3314
                     /gene="metG"
                     /locus_tag="VIF63_07335"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY8239233.1"
                     /translation="MPDRPRYYLTTAIAYPNNKPGLHTLYEVIGADVVARWHRMKGDD
                     TRFLTGTDEHSVNIAERAKELGRDTREFVDEMVALFTDAEGQLLIAPDRFIRTTDPDH
                     RRASQEMVRRAFANGDVYLGKYEGWYCPNEGFKAPSDLHETPDGMRCPNHPNITLQWL
                     TERNWFFRLSAYRDRLVAHFEANPDWVQPEYRKNEMLAFLRGGLEDISISRETFHWGI
                     PFPIAENGETAEREDGSWDPEAGVIYVWFDALINYITGAGFPDDPEAFAKWWPADLHV
                     IGKDITRFHTIIWPAMLMSAGIALPRKVWVHGFLMAAGGERMSKSRGNMFDPTDVVTA
                     LGADGARYVAMREVPFDKDSEVSWDSFVRRYNADLANDFGNLVNRTVSMAGRYLGGAR
                     PAPRVAAEAPLAPRWAVAVETYAAKLDALLLHEALAALWDFVNDANRFVEAEQPWALA
                     KAAKAGDDVAPAAEARLAGVLGDLVEAARLISLAVAPFMPSIAPRALEQLGHAYGYAA
                     DGNGGPALLEQLEWGAASGAPGRLGTPAPLFPRLETEAAAAEAAAAGDAPA"
     gene            3426..3791
                     /locus_tag="VIF63_07340"
     CDS             3426..3791
                     /locus_tag="VIF63_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8239234.1"
                     /translation="MSKHGEFSHIEFPADDVERAKSFYANVFGWSFESMDGFENYFLY
                     TAGPGELGGGIGVRGQNAPATVRNYIGVDDVDSAVAKVETNGGSVVVPKTDIGVGWYA
                     AVKDTEGNELGLYKSKRDA"
     gene            complement(3798..4313)
                     /locus_tag="VIF63_07345"
     CDS             complement(3798..4313)
                     /locus_tag="VIF63_07345"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEY8239235.1"
                     /translation="MTGKQATGSKARSKSVEPSPMGRLGETIGRIPRYLMLAAALLRD
                     PKLGRWRKAGLAASLAYVASPIDFVPGIIPVAGQLDDLAALLLGIRNALRGCSPTGAA
                     AHLKKARLTATDIDNDLATVRASAGWIAGHVGAATVRLGRFSAKGAGKLLGIGARGAR
                     TLAAEAAKRVR"
     gene            4354..5205
                     /locus_tag="VIF63_07350"
     CDS             4354..5205
                     /locus_tag="VIF63_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8239236.1"
                     /translation="MRLIDSHCHLNAERFAGDEAAVLERARSAGVERILVPGWNLASC
                     ERAIELADRFDWLDIAVGVHPHDAAKIDDAGWRRIVELAAHPRVVAVGETGLDYDRVF
                     SPIPDQLANLQRNLALSLETGKPAILHVRSAGRNADAQDAILAELRAAGFGGQASVGA
                     FGPDRPPAVIHSFSGSVDYARQVLDLGLAISISGLAFRTGEEATADVVAVVPADGLLV
                     ETDSPFLSPPGGPRGRNEPEFVRLTATWVAERRAAASSDDQVWPGDSLVRAYDRALRS
                     RVAPGPG"
     gene            5409..6440
                     /locus_tag="VIF63_07355"
     CDS             5409..6440
                     /locus_tag="VIF63_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8239237.1"
                     /translation="MGGRPLREKIASMLVVGFRGFDLDNAAWVRKAIREHGLGGVILF
                     DRDGQTKGPRNVKSAVQVRALSTEIKALAPERTVIVAIDQEGGLVTRLSPKYGFPAVE
                     SQAAIGEQGDVAVSQWARGIAATLVGAGVNLNFAPVVDLNVNPDNPAIGALDRAFSAD
                     PAIVIRDTELEITAHHERGVRTALKHFPGLGSATANTDFGVADVTDTWTDVELEPYRT
                     LIKSAAVDMIMAAHVVNGQIDPNGPASLSKATVTDLLRGDLGYRGVVVTDDMQAGAIT
                     DAFGADDAVFLAIDAGCDLLVFANQQVYEPARAEAVIDLIERLVGEERLTEARIDESV
                     ARIDALFQY"
     gene            6693..7007
                     /gene="groES"
                     /locus_tag="VIF63_07360"
     CDS             6693..7007
                     /gene="groES"
                     /locus_tag="VIF63_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8239238.1"
                     /translation="MATATKTETKLQPLGDRVVLKPTPRDEMTKSGIVLPDTAKEKPQ
                     EGMILAIGPGAFDAEGKRVAMDVKVGQKVLYGKYAGTEFKLEDEELLIVSQKDILAIV
                     AD"
     gene            7058..8692
                     /gene="groL"
                     /locus_tag="VIF63_07365"
     CDS             7058..8692
                     /gene="groL"
                     /locus_tag="VIF63_07365"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8239239.1"
                     /translation="MAKQLYFDETARRSLKRGIDRLADAVKVTIGPKGRNVVLDKKFG
                     SPTITNDGVTIARDIELDDPFENMGAQLLKEVATKTDDVAGDGTTTAVVLGQAMVAEG
                     LRVVTAGANPMVVKRGIEKAVEAIVEEIKRVSRPVDNREQIAAVAAISAADPEVGEII
                     AEVMDKVGKDGVITVEEGQSLGLEKEYTEGMQFDRGYISAYFVTNPDRMETVLENVLI
                     LITDKKISSIQDMLPALEKGVQQGRPILIIAEDVDGEALATLVVNKLKGTISVLAVKA
                     PGFGDRRKEMLRDIATLTGGTVISEEIGRKLDSVTAEDFGKARRVVATKDDTTIVDGE
                     GSPDAIKARMTQIKAQIEDTTSDYDREKLQERLAKLAGGVAVIKVGAATEVELKEKKH
                     RIEDALSTTRAAVEEGIVAGGGTTLLQAIPALDKLKLGGDAQVGVDIVRKALEAPARQ
                     IADNAGQPGEVVVSKVRGLPTGEGYDALAGEYGDMFAKGIVDAAKVTRSALQNAASIA
                     AMVLTTETLVTDIPEKQAAGAGGGHGHGGGGGDMDF"
     gene            8854..9873
                     /locus_tag="VIF63_07370"
     CDS             8854..9873
                     /locus_tag="VIF63_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY8239240.1"
                     /translation="MQREADSAGQVTPARGAGVRIRLFDADRTDRTLSLEEALETKPS
                     ARQLLWIDVAGEAAPAEWRALADRFELDAETARALGETGTQPRIQLHDKHFHLRVAAE
                     PDPSRRQGASWLDLVAGPNVVISQHHDELGFLGGINDQIAADATIGDLDSAEFVASVL
                     DSIVTTYHAAIDGLEDELDEIDSRALAGRHTDEQFGRLVTIRRRIARLRRLLAAHREL
                     FSVLGRRDFGRGIESADPEVFLPVAARFEGAILSLESTRDVVLGSFDVLMTRAAQRTN
                     DIMRTLTLVTVMAVPATITAGFLGMNVIVPVSKDDPGSFWLIVVLVLVLEAAILAIAR
                     WRRWI"
     gene            9928..>11412
                     /locus_tag="VIF63_07375"
     CDS             9928..>11412
                     /locus_tag="VIF63_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8239241.1"
                     /translation="MSPTPRRSARDRPVDAVEELEGTRFEELPPAVPGEAAAALPDEP
                     IWLSDAPESQDPEAWRDPKVQALQAKRLAEIADSRRQEILSRLNPEQERAVTTTDGPV
                     LILAGAGSGKTRVLAHRIAYLVGVKNVPPFRILAVTFTNRAAGELRERIIRLVGEPGK
                     EVQAGTFHSICARVLRRDGEQIGLDRRFVIYDTDDQQQLMKQILREEGLDAKGETRPA
                     AILGAISRAKNDMVDPGEIPPLRLATGVTVQIARLARLYVAALKKANAVDFDDLLLDA
                     VRLFDESPATLARYQDRWQYLHVDEYQDTNRPQYLWIRALAAKHRNLAVVGDDDQSIY
                     GWRGADIRNILDFERDWPDATVIKLEQNYRSTQLILDAAHAVVSKNVARTDKKLWTKN
                     DRGRLIERFEAYNEEEEAEWIARRVEELTGTRGSPLTRKADDGAKLPLRDIAVLYRTN
                     AQSRAIEEACLRYGIRYQVVGGTRFYSRREVKDALAYLRVLRSDA"
BASE COUNT         1850 a         4006 c         3914 g         1642 t
ORIGIN      
        1 gaacccgcag cagcccgccg cgcgctatcg acgtgtcgac cggcgagcga ggcccgcgga
       61 gggcccgttc gcgaccggca cgccgccgct ccagggcaag gcgctgtcgt acaacaacgt
      121 gctcgacgcc tcggctgcgg gcgccgccgc gcgcctgctc cacgggccag cgtgcgtgat
      181 cgtgaagcac acgaacccgt gtggcgccgc cgagcgctcg accctcttcg aggcgtggca
      241 ggcggccctc accggcgacc cggacgccgc cttcggtggg gtggtggcgc tgacgggcct
      301 cgtcaccgcc gacgtcgccc gagggctcac ggagatcttc ctcgaggtcg tcgtggcgcc
      361 cgccttcgaa ccggaggccc tcgagatcct tgccgcgaag cagaacctgc gcctgatcga
      421 agacccaaac ctcaccggga tcccggcgaa agcacgcgcc gatggcggcc tgaccgtcga
      481 ctacctgggc agcgtgcgca gcgtcggtgg cgcgatcctc gtcggcgcgc cggacgattc
      541 gatcgacgac ccgggcgact ggctcaccct cacctcgcga gcacccacgg agcgcgagca
      601 ggccgatctc gatctcgcct ggcgcctcgc ccgcggcgtc gtctcgaacg cgatcgtgct
      661 cgtgcgcgac cgacagctcg tcggcctggg atcggggcag gtgagccgcg tcgacgcctg
      721 ccggcaagcg gtcgtgaagg ccgagcgatt ccatgggccg gtgggcgccg ccggggccgt
      781 tgccgcctcg gacgccttct tcccgttcgc ggacggcccg caggtgctgc tcgatgccgg
      841 cgtgacggcg atcgtgcagc ccggcggctc gctgcgtgac gcggaggtcc tcgcggcggt
      901 cgacgaggcc ggcgccgtga tgcagatcac cgcgcgccgc cacttccgtc actgacccgg
      961 gcaggggata ctggcgccat gtcgaagctg acgaacgagg aatgcgtggc gagatacggc
     1021 gccgcgtccg ccgcgcacga cgtggccgcg ctggcggccc tgcgccaccc cgaatggagc
     1081 gtgtactggc cgcagtcggg cgagcgcgtg cacacctcgg aggcctttgc cgagatcatc
     1141 gacaactacc cgggcggcgc accgtcgacc gaggtgactc gcgtcgtcgg ctccgaggac
     1201 cagtgggtcg tcactgcggg caatacggtg atgctcgtcg ccgggagcgg cgacttctgg
     1261 tggacggagt ggaagatcac gtatccggac ggcgaggtgt acctcgtcgt cgatctcatg
     1321 gagctgcgcg acgggctcgt ccatcgagag acggtctact gggcggcgcc gttcgaggcg
     1381 cccgcctggc gcgcgccgtg ggtcgagcgg acctgacgcg aacgtcgggc tggtaccgct
     1441 tgaccgcgcc gccgtgcagc gtcgatgatc gcgccgtgat ccgaccccgg agcccgatgg
     1501 cgatgccggg gcggtcgatc ttccggcggg cctgaccgag acacccgacg gccgatcggc
     1561 cgccgtctcg agcctgcgcg cggcggatcg cgcgcgacca gttgacccgt ccgaccctcc
     1621 cgcacgagga ccccatgccc gaccgtcccc gctactacct gacgaccgcc atcgcctacc
     1681 cgaacaacaa gcccggtctg cacaccctgt acgaggtgat cggggcggac gtcgtcgcgc
     1741 gctggcatcg gatgaagggc gacgacacgc gcttcctcac cggcacggac gaacactcgg
     1801 tgaacatcgc cgagcgcgcc aaggagctcg gacgggacac ccgcgagttc gtcgacgaga
     1861 tggtcgcgct gttcaccgac gccgagggcc agctgctcat tgcaccggac cggttcatcc
     1921 gcacgaccga tcccgaccat cgccgcgcgt cccaggagat ggttcggcga gcgttcgcca
     1981 acggcgacgt ctacctcggc aagtacgaag gctggtactg cccgaacgag ggcttcaagg
     2041 cgccgtcgga cctgcacgag acgcccgacg gcatgcgctg cccgaaccac ccgaacatca
     2101 ccctccagtg gctcacggag cgcaactggt tcttccgcct gtcggcgtac cgcgaccggc
     2161 tcgtcgccca tttcgaggcg aacccggact gggtccagcc ggaataccgc aagaacgaga
     2221 tgctcgcctt cctgcgcggc ggcctcgagg acatctcgat ctcgcgcgag accttccact
     2281 ggggcatccc gttcccgatc gccgagaacg gtgagacggc ggagcgcgag gacggctcct
     2341 gggatcccga ggcgggcgtg atctacgtct ggttcgacgc gctcatcaac tacatcaccg
     2401 gcgcgggctt ccccgacgac cccgaggcat tcgcgaagtg gtggccggcc gacctccacg
     2461 tcatcggcaa ggacatcacc cgcttccaca cgatcatctg gccggcgatg ctcatgagcg
     2521 ccggcattgc attgccgcgg aaggtctggg tccacggctt cctcatggct gcgggcggcg
     2581 agcgcatgag caaaagccgc ggcaacatgt tcgacccgac cgatgtcgtc accgcgctcg
     2641 gcgccgacgg cgcccgctac gtcgccatgc gcgaggttcc cttcgacaag gacagcgagg
     2701 tgtcctggga ctcgttcgtt cgccgctaca acgcggatct cgccaacgac ttcggcaacc
     2761 tggtgaaccg gaccgtgtcg atggccggcc gctacctcgg tggcgcccga cccgcgccac
     2821 gcgtggctgc cgaggcgccg ctggctcccc gctgggccgt tgcggtcgaa acctacgcgg
     2881 cgaagctcga cgcgttgctc ctccacgaag ccctcgcggc gctgtgggac ttcgtgaacg
     2941 acgcgaaccg cttcgtcgag gcggagcagc cgtgggcgct cgccaaggcc gccaaagctg
     3001 gggacgacgt tgctccggcg gccgaggcgc gtctcgcggg cgtgctcggt gacctcgtcg
     3061 aggccgcgcg gctgatctcg ctcgccgtcg cgccgttcat gccctcgatc gcgccgcgcg
     3121 ccctcgagca gctcggccac gcctacggct acgcggccga tggcaacggc gggccggcgc
     3181 tcctcgagca gctggaatgg ggggctgcgt ccggcgctcc gggccggctc ggcacgccgg
     3241 cgccgctgtt cccgcgactc gagacggagg ccgcggccgc cgaggccgct gcggccgggg
     3301 acgcgcccgc ctgaggcgtg catttcgggc gaatctcctc cgtttctgcc gagacctgtc
     3361 gattcgaatg gcacctgttc gtcaatttca acgaagggcg aattcgtcgc ccgaaggagg
     3421 acgtgatgtc gaagcacggt gagttcagcc acatcgagtt cccggcggat gacgtcgagc
     3481 gggcgaagtc gttctacgcg aacgtcttcg gctggtcgtt cgagtcgatg gacggattcg
     3541 agaactactt cctgtacacg gccggccccg gcgagctcgg cggtggcatc ggcgtccgcg
     3601 gccagaacgc gccggcgacg gtccgcaact acatcggcgt cgatgatgtc gatagcgccg
     3661 tcgcgaaggt cgagaccaac ggcggctccg tcgtcgtgcc caagaccgac atcggcgtcg
     3721 gctggtacgc ggcggtcaag gacaccgagg gcaacgagct cggcctgtac aagtcgaagc
     3781 gcgacgcctg attcggccta gcgaacccgt ttcgcggctt cggccgcgag cgttcgggcg
     3841 ccgcgcgccc cgatcccgag cagcttcccg gcgcccttcg ccgagaaccg gcccagcctg
     3901 acggtcgcgg caccgacatg accggcgatc cagcctgccg aggcgcggac cgtcgcaagg
     3961 tcgttgtcga tgtccgtggc cgtcaggcga gctttcttca ggtgcgccgc cgcgccggtg
     4021 ggactgcacc ctcgcagcgc gttccggatg ccgaggagca gggcagccag gtcgtcgagc
     4081 tggccggcga ccgggatgat tccgggcacg aagtcgatcg gcgacgccac gtaggcgagg
     4141 ctggccgcga ggcccgcctt ccgccaccga cccagcttcg ggtcgcgcaa cagggcggcc
     4201 gcgagcatga gatagcgcgg gatgcgcccg atcgtctcgc ccagccgccc catcggcgac
     4261 ggctctaccg acttcgagcg cgccttcgac cctgtcgcct gcttcccggt catgtcccga
     4321 accctacgcc gtgtccgcgc ctagcatcga cgggtgcgcc tgatcgacag ccactgccac
     4381 ctcaatgccg agcggttcgc gggcgacgag gcggctgtgc tcgagcgagc ccggtcggcg
     4441 ggcgtcgagc ggatcctcgt gcccggctgg aacctcgctt cgtgcgagcg cgcgatcgag
     4501 ctggcagatc gcttcgactg gctcgacatc gccgtcggcg tgcatccgca cgacgccgca
     4561 aagatcgacg acgctggctg gcgccgcatc gtggagctcg cggcgcaccc gcgcgtcgtc
     4621 gcggtggggg agaccgggct cgactacgac cgggtcttct cgccgatccc ggaccagctc
     4681 gccaacctcc agcgaaacct cgccctgtcc ctcgaaacgg gcaagccggc gatcctccac
     4741 gtccgatcgg cggggcgcaa tgccgacgcc caggatgcga tcctggccga gctgcgagcg
     4801 gcgggcttcg gcggccaggc gtcggtcggt gcgttcgggc cggatcggcc gcccgcggta
     4861 attcattcgt tctccgggtc cgtcgactat gcccgacagg tgctcgatct cggcttggcg
     4921 atctccattt ccgggctcgc gttccggacc ggtgaggagg cgacggcgga cgtcgtagcg
     4981 gttgttccgg cagacggcct gctggtggag accgattcac cgttcctttc gccgccaggc
     5041 ggtccgcgag gtcggaacga gccggagttc gtcaggctga ccgcgacgtg ggtcgcggag
     5101 cgacgtgcag cagcgagctc ggacgaccag gtctggccag gcgactccct cgtacgcgcg
     5161 tacgaccgcg cgttgcgatc gcgcgtggcg ccggggccag gttgaacgct ccgacgctca
     5221 gtcgacgtcg cttcctagcg ggcctggcag ccggggcggc gacgctagcc ctggaggcgt
     5281 gcgctcccac cgccccaagt ccgtccgcgc cttcgaccgg accctcgccc tccgcggccc
     5341 cggttcgatc cgcgctgcca tcggcgccgc cgacggcggg cgcaacaacc gcgccgtcgc
     5401 cgacgccggt gggcggccgc ccactgcgcg agaagatcgc ctcgatgctg gtcgtcggct
     5461 ttcgcggctt cgacctggac aacgccgcgt gggtgcgcaa ggcgattcgg gagcacggcc
     5521 tgggtggcgt catcctcttc gaccgcgacg ggcagacgaa agggccgcga aacgtcaaat
     5581 cggccgtcca ggtccgcgcc ttgagcacgg agatcaaggc gctcgcaccg gagcgcacgg
     5641 tcatcgtcgc catcgaccag gagggcggcc tcgtcacgcg cctgtcgccg aaatacggct
     5701 ttccggccgt cgaatcacag gcagcaatcg gcgaacaggg cgacgtcgcg gtgagccagt
     5761 gggcgcgcgg gatcgcggcg acccttgtcg gcgcgggcgt caacctgaac tttgctccgg
     5821 tcgtggacct gaacgtcaac ccggacaatc cggccatcgg ggcgctcgat cgagccttct
     5881 cggccgatcc cgcgatcgtc atccgcgaca cggagctcga gatcaccgcc caccacgagc
     5941 gcggtgttcg cacggcgttg aagcacttcc ccggcctcgg cagcgcgacg gcgaacaccg
     6001 atttcggggt cgccgacgtg accgacacct ggacggatgt cgagctcgag ccataccgga
     6061 cgctgatcaa atcggcggca gtcgacatga tcatggccgc gcatgtcgtc aacggccaga
     6121 tcgacccgaa cgggcctgcg tcgctctcga aggccacggt caccgacctg ctgcgcggcg
     6181 acctcggcta tcgcggcgta gtcgtgaccg acgacatgca ggctggcgcc atcaccgacg
     6241 cattcggtgc cgacgacgca gtcttcctcg cgatcgacgc cggctgcgac ttgctcgtct
     6301 tcgcgaacca gcaggtctac gagccggctc gcgccgaggc ggtgatcgac ctcatcgaac
     6361 gcctggtggg ggaggagcgg ctcacggagg ctcgaatcga cgagtccgtc gctcgcatcg
     6421 acgcgctctt ccagtactga gcggcagccg ttcgagggat gcggtttgac acccgtggag
     6481 cccggctcct actataacta gcactctcac cctgagagtg ctaatcggct cggttccaaa
     6541 cgtccgctgg gctccgcacg gagattgccg gaggcgcgga tcgggactcg ccgaaccacc
     6601 accagcgcgg gttgggcccg cttcatcacc cgggcgtgca gcgccctgat cgaaggctcg
     6661 gacggctccg gccgtcccca ggagggaaca ccttggcaac cgccacgaag accgagacca
     6721 agctccagcc gctcggcgac cgcgtcgtgc tcaagccgac gccccgcgac gagatgacca
     6781 agagcggcat cgtcctgccg gacaccgcga aggagaagcc ccaggaaggg atgatcctcg
     6841 ccatcgggcc gggcgccttc gacgctgagg gcaagcgcgt cgcgatggac gtcaaggtcg
     6901 gccagaaggt gctctacggc aagtacgccg gcaccgagtt caagctcgag gatgaggagc
     6961 tcctcatcgt cagccaaaag gacatcctcg cgatcgtcgc cgactgacgt cgggcgttcg
     7021 cagccacccc acccgaagac accctggagg catcaccttg gcaaagcagc tgtatttcga
     7081 tgagaccgcc cgccggtcgc tcaagcgcgg catcgaccgc ctcgccgacg cggtgaaggt
     7141 cacgatcggt ccgaagggcc gcaacgtggt cctcgacaag aagttcggca gccccacgat
     7201 caccaacgac ggcgtcacga tcgctcgcga catcgagctc gacgacccgt tcgagaacat
     7261 gggcgcccag ctcctcaagg aagtcgccac caagaccgac gacgtcgccg gcgacggtac
     7321 caccaccgcg gtcgtgctcg gccaggcgat ggtcgccgag ggcctgcgcg tcgtgaccgc
     7381 cggggccaac ccgatggtcg tcaagcgcgg catcgagaag gcggtcgagg cgatcgtcga
     7441 ggagatcaag cgcgtgtccc gcccggtcga caaccgcgag cagatcgcgg ctgtcgccgc
     7501 catctcggcc gcggatcctg aggtcggcga gatcatcgcc gaggtgatgg acaaggtcgg
     7561 caaggacggc gtcatcaccg tcgaagaggg ccagagcctg ggcctcgaga aggaatacac
     7621 cgagggcatg cagttcgacc gaggctatat ctcggcgtac ttcgtgacca acccggaccg
     7681 gatggagacc gttctcgaga acgtcctcat cctcatcacg gacaagaaga tctcgagcat
     7741 ccaggacatg ctgcctgcgc tcgagaaggg cgtccagcag ggccgcccga tcctcatcat
     7801 cgccgaggat gtcgatggcg aggccctcgc cacgctcgtc gtcaacaagc tgaagggcac
     7861 gatctcggtc ctcgccgtca aggcgccggg cttcggtgat cgccgcaagg agatgcttcg
     7921 cgacatcgcg acgctgaccg gcggaaccgt catcagcgag gagatcggcc gcaagctcga
     7981 ctccgtcacc gccgaggact tcggcaaggc ccgccgcgtc gtcgccacca aggacgacac
     8041 gacgatcgtc gacggcgagg gttcgcccga cgcgatcaag gcgcgcatga cccagatcaa
     8101 ggcgcagatc gaggacacga cctccgacta cgaccgcgag aagctccagg agcggctcgc
     8161 gaagctcgca ggcggtgttg ccgtgatcaa ggtcggcgct gcgaccgagg tcgagctcaa
     8221 ggagaagaag caccgcatcg aggacgcact ctcgacgacc cgcgcggccg tcgaagaggg
     8281 catcgtcgcc ggcggcggca cgaccctcct gcaggcaatc cccgccctcg acaagctcaa
     8341 gctcggtggc gacgcccagg ttggcgtcga catcgtgcgc aaggcgctcg aggctccggc
     8401 ccgccagatc gccgacaacg ccggccagcc cggtgaagtc gtggtctcga aggtccgcgg
     8461 cctgccgacc ggcgagggct acgacgccct ggccggcgag tacggcgaca tgttcgccaa
     8521 gggcatcgtg gacgccgcca aggtgacccg gtccgccctc cagaacgccg cctccatcgc
     8581 cgcgatggtc ctgacgacgg aaacgctcgt caccgacatc ccggagaagc aggcagccgg
     8641 cgccggcggt ggccacggcc acggcggcgg cggcggggac atggacttct agtccactcc
     8701 cttcgcaaac gcaggagccc gggccggcaa cggcccgggc tcttcgattc cgtcgctgaa
     8761 cggtgttgcg ggctgcgcga ttgcactcgt ttgtatcgtt gcgggcactt cccgcgagcc
     8821 ggagcacggt gccgatgggc caggatcggg ctggtgcagc gcgaggccga cagcgcgggt
     8881 caggtgacgc ccgcaagggg agcgggcgta cggattcggc ttttcgacgc cgaccggacg
     8941 gaccggacgc tctcgctcga ggaagcgctc gagaccaaac catcggcgcg ccagctgctg
     9001 tggatcgacg tcgcgggcga ggcggcgccg gcggagtggc gtgctctcgc ggacaggttc
     9061 gagctcgacg cggaaaccgc acgtgcactc ggggagaccg gcacacagcc gcgcatccag
     9121 ctccacgaca agcatttcca cctccgcgtc gccgcggagc ccgatccgag tcgccgacag
     9181 ggcgcgagct ggctcgacct cgtggcgggt cccaacgtgg tgatctcaca gcaccacgac
     9241 gagctgggct tcctcggcgg gatcaatgac cagatcgcgg cggatgccac gatcggggat
     9301 ctggactcgg cggaattcgt ggcgtcggtc ctggactcga tcgtgacgac gtaccacgct
     9361 gcgatcgacg gcctcgagga cgagctcgac gagatcgact cgagggcgct ggccgggcgg
     9421 cacacggacg agcagttcgg acggctcgtg accatccgcc ggaggatcgc gcggctccgg
     9481 cgcctgcttg ccgcccaccg cgagctgttc agtgtcctgg gcaggcgcga cttcgggcgg
     9541 ggcatcgaat cggccgatcc agaggtgttc ctgcctgtcg cggcgaggtt cgagggcgcg
     9601 atcctgtcgc tcgaatcgac gcgagatgtc gtgcttggct cgttcgacgt cctcatgacc
     9661 cgcgccgccc agcggacgaa cgacatcatg cgcacgctca cgctcgtgac ggtcatggcc
     9721 gtgcccgcca cgatcaccgc cgggttcctc gggatgaacg tgatcgtgcc cgtctcgaag
     9781 gacgatccgg ggtcgttctg gctcatcgtc gtcctggtcc tcgtcctcga ggccgcgatc
     9841 ctcgcgatcg cccgctggcg ccgctggatc tgacgcgcgc ggcgccctga tgaccgggag
     9901 gaaaggtcgg ccgactagaa ttccctggtg agcccaaccc cacgccggag cgcacgcgac
     9961 cggccggtcg atgctgtcga agagctggaa gggacgcggt tcgaggagct tccgccggcc
    10021 gtgccgggcg aggcggcggc tgcgctgccc gacgagccga tctggctctc ggatgctccc
    10081 gaatcccagg atcccgaggc gtggcgcgac cccaaggtgc aggccctcca ggcgaagcgt
    10141 ctggcggaga tcgcggactc gcggcgccag gagatcctga gccgcctcaa ccccgagcag
    10201 gagcgtgcgg tgacgaccac cgacggtcct gtgctgatcc tcgcgggtgc cggctccggc
    10261 aagacccgcg tcctcgcgca ccggatcgcg tacctcgtcg gggtcaagaa cgtaccgccg
    10321 ttccggatcc tcgcggtcac gttcacgaac cgcgccgcgg gtgagctgcg cgagcggatc
    10381 atccggctcg tcggcgagcc cggcaaggag gtccaggctg ggacgttcca ctcgatctgc
    10441 gcccgcgtgc tgcgccggga cggcgaacag atcgggctgg atcgccgctt cgtgatctac
    10501 gacacggacg accagcagca gctgatgaag cagatcctgc gcgaggaggg cctcgacgcg
    10561 aagggcgaga cccggcccgc ggccatcctc ggtgcgatca gccgggccaa gaacgacatg
    10621 gtcgatccgg gcgagatccc gccgctccga ctcgccaccg gcgtgaccgt ccagatcgcc
    10681 cgcctcgccc ggctgtacgt ggcggcgctc aagaaggcca acgcggtcga cttcgacgac
    10741 ctcctgctcg atgccgtccg cctcttcgac gagtcgcccg cgacgctcgc tcgctaccag
    10801 gaccgctggc aatacctcca cgtggacgag taccaggaca cgaaccggcc gcagtacctc
    10861 tggatccggg cgctcgcggc gaagcacagg aacctggcgg tggtcgggga cgacgaccag
    10921 tcgatctacg gctggcgggg cgcggacatc cgcaacatcc tcgacttcga gcgcgactgg
    10981 cccgatgcga cggtcatcaa gctcgagcag aactaccgct cgacccagct gatcctcgac
    11041 gccgcccatg ccgtcgtgtc gaagaacgtg gcccggacgg acaagaagct gtggacgaag
    11101 aacgatcgcg gccggctcat cgagcgcttc gaggcgtaca acgaggaaga ggaggccgag
    11161 tggatcgccc gccgcgtcga ggagctgacc gggacgcgcg gctcgccact gacccggaag
    11221 gcggacgacg gcgccaagct gcccctgcgc gacatcgccg tgctgtaccg gacgaacgcc
    11281 cagtcgaggg cgatcgagga ggcgtgcctc cgctacggga tccgctacca ggtcgtcggc
    11341 gggacgcggt tctactcgcg gcgcgaggtc aaggacgcgc tcgcatacct gcgcgtgctg
    11401 cgttcggacg cg
//
LOCUS       DATBSW010000260         7056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_60771, whole genome shotgun sequence.
ACCESSION   DATBSW010000260 DATBSW010000000
VERSION     DATBSW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60771"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VIF63_07380"
     CDS             complement(<1..1010)
                     /locus_tag="VIF63_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8239242.1"
                     /translation="MRTLNLGILAHVDAGKTTLTERLLFAAGVIHKLGSVDEGTTQTD
                     TLDLERQRGITIKAAVASFAIDDLTVNILDTPGHPDFIAEVERVLRVLDGAILVISAV
                     EGVQPQTPLLYRALARLAVPTVLFLNKLDRRGADPERTLDQIAKRLTPAIVPVRTAAE
                     PGTPDVTIVEPAETDETYRGLLVERLAERDESLLTAYVETGSVPWPRLRRALADQSRR
                     GVIHPVFMGSAATGVGVEELLAAIPDLLPAATGDPEADVRGRVFKIERGPGGDHVAYA
                     RIFDGTVHARDRLAFGAGAEARVTHLAVTTAGGATQQQEVSVGQIAKLGGLGGIRVGD
                     PIG"
     gene            complement(1251..2684)
                     /locus_tag="VIF63_07385"
     CDS             complement(1251..2684)
                     /locus_tag="VIF63_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8239243.1"
                     /translation="MDDRRALLIRTAELAIDFLDRLPNRPVGRKTDLEKLRAAFGGPL
                     PEDPQAPLGVVERLAQSAEPGLVGTAGSRYFGFVVGGSVPAALAADWLTSAWDQNTGL
                     YVLSPAGAVAEEVAATWLIELFGLPKGSSVGFTTGATMATFTAIAAARHAVLERAGWH
                     VEDDGYVGAPPIEVVVSDESHVTIFASLQMLGLGRKRVHRVAVDEQGRMRADALRETL
                     ARIDGPTIVCAQAGNVNTGSFDPLPEIVEAVRSKPNAWLHVDGAFGLWAAVVPDMRDR
                     VAGIEGADSWTTDAHKWLNVPYDSGIVICRDPAPHHAAMTLGAAYYVETAGGERDNYN
                     WVPESSRRARGFTVYAALRSLGRSGLGEMIGGNAAIARRMADRLAEAPGVVILNKVVL
                     NQVLVRFVPPGAAEEAASAPDPTVDDFTRRVIAAVQADGTCWAGGTTWHGMGAMRISV
                     SNWSTTEADADLSVEAMLRCAREIAAS"
     gene            2720..4360
                     /gene="dcd"
                     /locus_tag="VIF63_07390"
     CDS             2720..4360
                     /gene="dcd"
                     /locus_tag="VIF63_07390"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY8239244.1"
                     /translation="MSVLSDRDIRASLDAGEIVIRPYDPQDLQPSSVDLHLDRSFRVF
                     RNNRYPYIDVRQKQPDLTELLTIADDEPFILHPGEFVLGQTLEWSELPNDLVARLEGK
                     ALALDTPVPTPQGWRTMGELEPGHRVFDESGAPTEVVAVTPPMIGRPCREVLFSDGAH
                     VIADASHQWVTVDKNGRRYGRATLKVRTTDEIAQTLRVGDEFNHHVPLAGPVEYPSRA
                     DLPIEPYTLGAWLGDGTTTKAEITAYDEEVLEQISGDGFTVRPVGYAPNLFRIGGVGH
                     TRSASNGRFERNGSLSSRLRNQGLLGGKYVPSTYLEAGPSQRLALLQGLMDTDGFVDD
                     VAGRVEFTSTNEDLADAIVELAASLGFRPFKTYGRAMLNGIDHGPKYRVKFTPDRPVF
                     RVRRKLARQKPADAHFHRFRAITNVREVGSVPVRCIEVASPRGVFLVSRSFIPTHNSS
                     LGRLGLLIHSTAGYVDPGWKGNLTLELSNVANLPIALYYGMKIGQISFFRMTSPVDRP
                     YGSPELGSKYQGQSEPTASAFHRDFAAANGADKPPPSE"
     gene            4405..4563
                     /locus_tag="VIF63_07395"
     CDS             4405..4563
                     /locus_tag="VIF63_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239245.1"
                     /translation="MFLIVWLLSATVQPGGAASGFEAGRQAGYALGQGLIAAVITWAI
                     LKYVLKRA"
     gene            4571..5455
                     /locus_tag="VIF63_07400"
     CDS             4571..5455
                     /locus_tag="VIF63_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8239246.1"
                     /translation="MNDAVDWSTDLGGGTSVSLVQAGHFRTDAGAIFGPVPRLLWDKL
                     VTEEIDHDHRILQALNCLLVETPAGRVLIETGIGERISQKTRDMRGYQGKPIVTAMTE
                     AGFDPSSVDVVVMSHLHFDHAGGLMLAGDGGRAFPNAKIVAQQAEWEIAFSDNSRIVA
                     SYDQLELRLVGAWGEAGMVDGDVEVLPGVSVIRTGGHSKGHQAIVVRGTGSKAGAAGT
                     DGRTLAFFGDLGMRPWSANPRWITAFDDFPLDSVGAKADLFARAVEEDWLIALSHEPL
                     TPIGRLTPDRDRYRFEPV"
     gene            complement(5762..6871)
                     /locus_tag="VIF63_07405"
     CDS             complement(5762..6871)
                     /locus_tag="VIF63_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY8239247.1"
                     /translation="MSPKRKAKTPSTAPSVAEMDALTRDARLVLGGRGYRSPGQMLDE
                     IPRDIEGDLYGTGGVVAELEAEVATILGKPAAVFVPSGTMGQQITLRVHADRRGRRSV
                     VWHPQCHLAVHELDAPKRLHGLDGRAVGGRDRLISLDDLKEVAEPPAALLLELPQRDL
                     GGQLPSWKELNAQVDWAHSVGAAAHLDGARLWQCAGFYKRPLAAIAAPFDTVYVSFYK
                     DIGALAGAAVAGPADVIAEVREWRQRHGGTLYDMWPHAASALFNLRQRLPRMPAYRTH
                     ALALVAAIHDVPGVQVIPDPPHGSMFHVVFHRDAAELQAAALRLATADRIWTWGRFHA
                     TEVPGISRVELSVGDATLEWTPAEFREVIGRLLSF"
     gene            complement(6868..>7056)
                     /locus_tag="VIF63_07410"
     CDS             complement(6868..>7056)
                     /locus_tag="VIF63_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239248.1"
                     /translation="VFALPTQFFINPDGVIVQIVNGPLTEVRAAQLVESILPPGPTPA
                     PTRTPGAPVTTLVPAVTP"
BASE COUNT         1122 a         2384 c         2443 g         1107 t
ORIGIN      
        1 ccgatcgggt caccgacgcg gatgccgccg aggccaccga gcttcgcaat ctgcccaaca
       61 gacacctcct gctgctgcgt cgctccgccc gcggtcgtga cggcgagatg cgtgacgcgc
      121 gcctccgcgc cggcgccgaa cgcaagcctg tcgcgcgcat ggacggtccc gtcgaagatc
      181 cgggcgtagg cgacgtgatc gccgcccggc ccccgctcga tcttgaagac gcggccgcgc
      241 acgtcggcct ccggatcgcc cgtggccgcc gggagcaggt cggggatggc ggcgaggagc
      301 tcctcgaccc cgacgccggt ggctgccgag cccatgaaca cggggtggat cacaccgcga
      361 cgcgattgat cggcgagggc gcgacgaagg cgcggccacg gtacggaccc cgtttcgacg
      421 tatgccgtca gaagtgattc gtcgcgctcg gcgagacgct cgacgagcag accgcgatac
      481 gtctcgtcgg tctccgccgg ctcgacgatg gtgacgtcgg gcgtgccggg ttcggccgcc
      541 gttcgaaccg gcacgatcgc cggcgtgagg cgcttcgcaa tctggtcgag cgtccgctcg
      601 ggatcagcgc cgcggcggtc gagcttgttg aggaagagca cggtcgggac ggccagccgc
      661 gccagcgccc gatagagcag gggcgtctgc ggctggacgc cttcgacggc cgagatcacg
      721 aggatcgcac catcgagaac gcgcaggacg cgctcgacct cggcgatgaa gtcggggtgc
      781 ccgggcgtgt cgaggatgtt gaccgtcagg tcatcgatcg cgaacgaggc gaccgcggcc
      841 ttgatcgtga tgccgcgctg gcgctcgagg tcgagggtgt cggtctgggt cgtgccttcg
      901 tcgacgctgc cgagcttgtg aataacgccg gcggcgaaga ggagtcgctc ggtgagggtt
      961 gtcttaccgg cgtcgacgtg agcgaggatg ccgagattca gggtgcgcat cgggtgtcat
     1021 gccttccatg caggttcgaa tgaccttctg gctggacatg actgccgtca ttgcacgctc
     1081 cttgctcttc gcggtgctcg cctagtgtcg tggcgcacgc aagattgcca cgtcggtcaa
     1141 gcctgcgcgg aaactggtcg tatgcgtggt ttgggacggg caccgtttcg ccctcggcaa
     1201 gaacctatgc ccattgctac ataaacgcgg caaccaaggg cgaaggtgag ctaggacgca
     1261 gcgatctcgc gggcgcagcg cagcatcgcc tcgaccgaca aatcggcgtc ggcttcggtc
     1321 gtcgaccagt tcgagaccga gattcgcatc gcgcccatgc cgtgccaggt cgtgccgccg
     1381 gcccagcagg tgccgtcggc ctggaccgcg gcgatgacgc ggcgggtgaa gtcgtcgacc
     1441 gtcggatccg gcgccgaagc agcttcctcc gcggcgcccg ggggtacgaa ccgcaccagc
     1501 acctggttca gcacgacctt gttgaggatc acgacgcccg gcgcctccgc gagtcgatcg
     1561 gccattcgcc gggcaatcgc tgcgttgccg ccgatcatct cgccgagccc cgaacgcccc
     1621 agcgaccgaa gcgccgcgta gacggtgaac ccacgcgccc gccgcgacga ctcgggcacc
     1681 cagttgtagt tgtcgcgctc gcccccggcg gtctcgacgt aatacgccgc accgagggtc
     1741 atcgccgcgt ggtgcggcgc tggatcgcga cagatcacga tgcccgagtc gtacggcacg
     1801 ttgagccact tgtgggcgtc cgtcgtccac gaatcggcgc cctcgatgcc cgcgacgcgg
     1861 tcgcgcatgt ccggcacgac ggccgcccag agcccgaacg cgccgtcgac gtgcagccac
     1921 gcgttcggct tcgagcggac ggcctcgacg atctccggca acgggtcgaa cgagccggtg
     1981 ttcacgttgc ccgcctgggc gcagacgatc gtcggcccgt cgatccgtgc cagtgtctcg
     2041 cgcagggcgt cggcccgcat gcggccctgc tcgtcgaccg ccacgcggtg cacgcgcttc
     2101 cgtccaagcc cgagcatctg caacgaggca aagatcgtga cgtgggactc gtcgctcacg
     2161 acgacctcga tcggcggcgc gcccacgtag ccgtcgtcct cgacgtgcca gcccgcgcgc
     2221 tccagcaccg cgtggcgtgc agcggcaatg gccgtgaagg tcgccatcgt ggcgcccgtc
     2281 gtgaagccga cgctcgaacc cttcggcagg ccgaacagct cgatgagcca ggtcgccgct
     2341 acctcttccg cgaccgcgcc cgccggcgag agcacgtaca gccctgtgtt ctggtcccag
     2401 gcgctcgtga gccagtcggc tgcgagcgcg gccggcacgc tgccgcccac cacgaacccg
     2461 aaataccgcg agccggccgt tccgacgagg cccggctcgg cgctttgtgc gagtcgctcc
     2521 acgacgccga ggggcgcctg cggatcctca ggcagcgggc cgccgaacgc ggcgcgcagc
     2581 ttctcgaggt cggtcttgcg cccgacgggc cggttcggca gccggtcgag gaagtcgatc
     2641 gccaactcgg cggtgcggat cagcagtgcg cgtcggtcgt ccatgccggg cagggtagcc
     2701 gcgtgcgatg attccgccga tgagtgtgct ttccgaccgc gacatccgag cctcgctcga
     2761 cgccggcgag atcgtgattc gaccgtacga cccgcaggac ctccagccgt cgtcggtcga
     2821 cctgcacctc gatcgcagct tccgcgtctt ccgcaacaac cgctacccgt acatcgacgt
     2881 tcgccagaag cagcccgacc tgaccgagct cctgacgatc gccgacgacg agccgttcat
     2941 cctccacccg ggcgaattcg tgctcggcca gacgctcgag tggagcgagc tgccaaacga
     3001 cctcgtcgcc aggctcgaag ggaaggcctt ggcgctcgat acgccggtgc cgacacctca
     3061 gggttggcgg acgatgggag aactcgagcc gggccatcgg gtattcgacg agtcaggtgc
     3121 tccaaccgag gttgtcgcgg tcacgccgcc gatgattggg cgaccgtgtc gcgaagtcct
     3181 cttctcggat ggcgcacacg tcatcgccga cgcttctcac cagtgggtaa cggtcgataa
     3241 gaatggtcgg cggtacggcc gagcaacgct caaggtccgg acgaccgacg agatcgcgca
     3301 aacccttcga gtcggagacg aattcaacca tcacgtcccg ctcgcgggac ccgttgagta
     3361 cccatctcga gcagatctgc caatcgagcc gtacacgctc ggcgcgtggc tgggtgacgg
     3421 cacgacaacg aaggcggaaa tcacggcgta cgacgaggag gtccttgagc agatcagcgg
     3481 cgacgggttc acggtccggc ccgtcggcta cgcgccgaac ctcttccgga ttggcggcgt
     3541 tggtcatacg cgcagcgcat cgaacggccg attcgagaga aacggatcgc tttcgagtcg
     3601 cttgcggaac cagggtctgt tgggcggcaa gtacgtcccg tcgacctatc tcgaggccgg
     3661 cccctcccag cggctcgcgc tgttgcaggg cctcatggat acggatggct tcgtggacga
     3721 cgttgccggt cgagtcgagt tcacgagcac gaacgaggac ctggccgacg ccattgtgga
     3781 gctcgcggcg agtttgggtt tccgaccgtt caagacgtat ggccgggcga tgctcaacgg
     3841 catcgaccac gggccgaaat accgcgtgaa gttcaccccc gatcgaccag tgttccgggt
     3901 tcggcgaaag ctggctcgac agaagccggc cgacgcgcac ttccatcggt tccgggcgat
     3961 cacaaatgtt cgcgaggttg gatccgtccc ggtccgatgc atcgaggttg cctcgccgcg
     4021 tggcgtcttc ctcgtgtcga gatcgttcat cccgacccac aacagttcgc ttggccggct
     4081 cggcctgctc atccactcga ccgctggcta tgtcgacccc ggctggaagg gcaacctcac
     4141 cctggagctg tcgaacgtcg ccaacctccc gatcgcgctc tactacggga tgaagatcgg
     4201 ccagatcagc ttcttccgga tgacgagccc ggtcgatcga ccgtatggaa gcccggagct
     4261 cggctcgaag taccagggcc agtccgagcc aaccgcgtcc gcctttcacc gcgacttcgc
     4321 tgctgcaaat ggggcggaca agccgccgcc gagcgagtag cgaccgtggg gcagcgagcc
     4381 tcgaatcgga cgatcatcat cgcggtgttc ctgatcgtgt ggctgctctc ggcaacggtg
     4441 cagccgggtg gcgcggcgtc gggcttcgag gcaggccggc aggccggcta tgcccttggg
     4501 cagggtctca tcgcggctgt gatcacatgg gccatcctca agtacgtgct caagcgcgcc
     4561 tgatcgctcg atgaacgacg cggtcgactg gtcgaccgat ctcgggggcg ggacgagcgt
     4621 ctcgctcgtc caggcgggcc acttccgcac tgacgccggg gcaatcttcg ggccggtgcc
     4681 gcggttgctc tgggacaagc tcgtgaccga ggagatcgac cacgaccacc ggatcctgca
     4741 ggccctgaac tgcctgctcg tcgagacgcc ggcggggcgc gtgctgatcg agaccgggat
     4801 cggcgagcgc atctcccaga agacgcgcga catgcgcggc taccagggca agccgatcgt
     4861 gaccgcgatg accgaggcgg gcttcgaccc ttcgagcgtc gacgtcgtcg tgatgagcca
     4921 cctgcatttc gatcacgccg gcggcctgat gctcgccggc gacggcgggc gggcgttccc
     4981 gaacgcgaag atcgtggcgc agcaggccga gtgggagatc gcgttcagcg acaacagccg
     5041 gatcgtcgcc tcgtatgacc agctcgagct ccggctcgtg ggagcctggg gcgaggccgg
     5101 gatggtcgac ggcgatgtcg aagtgctgcc cggcgtctcg gtgatccgca cgggcggcca
     5161 ctcgaagggc catcaggcga tcgtcgtgcg cggcacggga tcgaaagccg gcgcggccgg
     5221 caccgacggg cggacccttg ccttcttcgg cgacctcggc atgcggccct ggagcgccaa
     5281 tccgcgctgg atcaccgcgt tcgacgactt cccgctcgac tcggtcggcg cgaaggcgga
     5341 cctgttcgcg cgcgccgtcg aagaggactg gctgatcgcg ttgagccacg agccgctgac
     5401 cccgatcggg cgcctgacgc cggaccgcga ccgctatcgg ttcgaacccg tctgaggtcc
     5461 cggcacgcga agcgcccgct catgatcgcc gggcgatcat tcttggggct tcagcgccaa
     5521 ttccacgcgc ggcatccgcc tcatcagcgg ctagacggcc ggtccacgct caagtcgatc
     5581 ggacggtcgg gttcgaatcc gtccgtatgg gcgtcgcacg gcctgaatgc cctcgcaacc
     5641 gccagaaatg atcgccacac gatcaccgat ggggacatgc tcgcgtgtcg gggagccgat
     5701 cacgatcggc ttgggcgcat gaggttgcgc aggttcagtt cgggggctcg ggccgctggg
     5761 ctcagaacga gagcaggcgg ccgatgacct cgcggaactc cgctggggtc cactcgagcg
     5821 ttgcgtcccc gacgctgagt tccacgcgcg agatgcccgg gacctccgtg gcatggaagc
     5881 ggccccaggt ccagattcga tcggccgttg cgaggcggag cgcggccgcc tggagctcgg
     5941 cggcgtcgcg atggaagacg acgtggaaca tcgaaccgtg gggcgggtcg gggattacct
     6001 gcacgcccgg cacgtcgtga atggccgcca ccagcgcgag ggcgtgcgtg cgatacgcgg
     6061 gcatccgcgg cagccgctgt cgcaggttga agagcgccga cgcggcgtgc ggccacatgt
     6121 cgtagagcgt gccgccgtgg cgctggcgcc actcgcgcac ctcggcgatg acatcggcgg
     6181 ggccggcgac cgcagcaccg gccagggcgc cgatgtcctt gtagaacgag acgtacaccg
     6241 tgtcgaacgg tgcggcgatc gccgcgagcg gccgcttgta gaagccggcg cactgccaca
     6301 gccgcgcccc gtcgaggtgg gcggccgcac cgaccgaatg cgcccagtcg acctgggcgt
     6361 tgagctcctt ccaggatggg agctgcccgc cgaggtcgcg ttgtggtagc tccagcagca
     6421 gcgccgctgg cggctcggcg acctccttga gatcgtcgag cgagatcagg cgatcccggc
     6481 cgccaaccgc ccgaccatcg agcccatgga ggcgcttggg cgcgtccagc tcgtggaccg
     6541 cgagatggca ttgcgggtgc cacacgaccg atcgacgccc tcgccggtcg gcatggactc
     6601 ggagcgtgat ctgctggccc atggtgccgc tcgggacgaa caccgcggcg ggcttgccga
     6661 ggatcgtggc gacctctgcc tcgagctcgg cgacgacacc accagtgcca taaaggtcgc
     6721 cttcgatgtc gcgcgggatc tcgtcgagca tctgtccggg cgatcgatag ccgcggccgc
     6781 cgagaacgag gcgcgcatcc cgcgtcagcg cgtccatctc cgcgacggag ggcgcggtgc
     6841 tgggggtctt tgccttgcgc ttggggctca cggcgtcacc gcgggcacca gcgtcgtcac
     6901 gggcgcccct ggggtgcgag ttggcgccgg cgtcgggccg ggcggcagga tcgattcgac
     6961 cagctgcgct gcgcgcacct cggtgagcgg cccgttgacg atctggacga tcacgccgtc
     7021 gggattgatg aagaactggg tcggcagcgc gaacac
//
LOCUS       DATBSW010000261         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1153074, whole genome shotgun sequence.
ACCESSION   DATBSW010000261 DATBSW010000000
VERSION     DATBSW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153074"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..356
                     /gene="rpmD"
                     /locus_tag="VIF63_07415"
     CDS             132..356
                     /gene="rpmD"
                     /locus_tag="VIF63_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY8239249.1"
                     /translation="MPGKSTGATLKVTQIKSSISHIARNRATLRALGLHGIGSSVEVP
                     ANDATRGMVRQVRFLVTVDETGAGEGKEKA"
     gene            353..853
                     /gene="rplO"
                     /locus_tag="VIF63_07420"
                     /pseudo
     CDS             353..853
                     /gene="rplO"
                     /locus_tag="VIF63_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            1280..>2529
                     /gene="secY"
                     /locus_tag="VIF63_07425"
     CDS             1280..>2529
                     /gene="secY"
                     /locus_tag="VIF63_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY8239250.1"
                     /translation="MFESLLNAFRAPDIRRRILFVLGILVVYRMLAQVPLPGVDRQAL
                     AEFFQNNSAFGIIDLFAGGGLSQLSIVGLAMNPYINASIIMQLMTGVIPSLQALSREG
                     EYGRQKLNQYTRYLTVPMALLQAFGILSAFAAQGVLAPSGEFGLGNAMSWVQMITLVA
                     GAVVLMWLGELITEKGIGNGISFIIFAGIVSRAPAGVLATLQQPNPRDIILFAILAIA
                     AVGVIIYIQEGQRRIPIQYASRVRGRRMYQGGQTFLPLRVNLAGVIPIIFAISILAFP
                     GQLAAYFQFSEVEGVRNVATAIGGFLAPTTIQYSIIYFLLTVGFTYFYTAFTFKPDET
                     AEQLRKNGGFIPGIRPGRPTQDYLARVVTRITFAGALFLAIVAVSPSLLALALPDLAT
                     LGLGGTSLLIVVSVVVETMKQIEA"
BASE COUNT          421 a          935 c          814 g          359 t
ORIGIN      
        1 caacccggtg aacgtgaccc gtgccaccct cgagggcctg cgcagcctca agtctgccgc
       61 cgagctcagc gcccggcgcg gcgtgcccgt tcgcctgtac atccccggcc agccgctcgc
      121 ggaggcagcg gatgcccggt aagtccactg gggcgaccct caaggtcacc cagatcaaga
      181 gctcgatcag ccacatcgcc cgcaaccggg ccacccttcg ggcgctcggc ctgcacggca
      241 tcggctcgtc ggtcgaagtg cccgccaacg acgcaacgcg gggcatggtc cgccaggtcc
      301 ggttcctcgt gaccgttgac gagacgggtg ccggcgaggg caaggagaag gcatgaagct
      361 ccacgatctt tcacccgcgc ccgggtcgcg caagaagcgc cgccgcgtcg gacgcggcat
      421 cgccgcgggc cagggcaaga ccgcgggtcg cggcacgaag ggccagaagg cccgagccgg
      481 cggcaagatt cccgcgtggt tcgagggcgg ccagacgccg ctccaccagc gcatcccgaa
      541 gctgcgcggc ttcaagaacc cgttcaaggt cgagttcgag gtcgtgaacc tcggcgacat
      601 cgcccgcctc gtcgagctcg gtgagctcga caacggcgac atgcccggca cgaagccctc
      661 gaagaagggc gccgcgccga tcacggtcaa ccaggagatc ctccgggccg caggcctcgt
      721 tcgccggctc gaccggccga tgaaggtcct cggcgcgggc gagctctcga cggccctgtt
      781 cgtcgtcgcc gacgcgttca gcgcgagcgc gaaggccaag atcgaggccg ccggcggcac
      841 cgtctcggtg ctcgaggtgc ccgaggcgaa gcgcccggca atcggcgtcg ccgccgactc
      901 gaaggctgac gccaaggccg aagcggttgc cgattcgaag gccgaagcca tcgccagcga
      961 tgacgttgcc gattccggtg ccgacgacga cgcggtcgcc gccgagcccg agtccgagcc
     1021 cgcggccccg gccaagcccg ccgccaaggc gaagggtgcc ccggcgaagg ccgccacggc
     1081 gaaggccgcc acggccgagg cgaaggcaac gactgcgaag gcaacgacta cgaaggcaac
     1141 cacggcgaag ccgaaggtaa cgactgccaa ggccgcgacg gccaaggctg ccgacaccgc
     1201 aaccgacgac gcccctgcaa cgccccgcgc cgcgcgcaag cccaaggcca ccaagccaga
     1261 cacggacgcg tagccgaccg tgttcgaatc gctgctcaac gccttccgcg caccggacat
     1321 tcgccgccgg atcctcttcg tcctggggat cctcgtcgtc taccggatgc tcgcgcaggt
     1381 gccgctgccg ggcgtcgacc ggcaggcact cgccgagttc ttccagaaca actccgcgtt
     1441 cgggatcatc gacctgttcg cgggcggcgg gctgtcgcag ctgagcatcg tcgggttggc
     1501 gatgaacccg tacatcaacg cctcgatcat catgcagctc atgacgggcg tgatcccgtc
     1561 gctccaggcg ctcagccgcg agggtgagta cggccgccag aagctgaacc agtacacccg
     1621 ctacctcacc gtcccgatgg ccctcctcca ggcattcggc atcctgtcgg cgttcgccgc
     1681 gcagggcgtc cttgccccga gcggcgagtt cggcctcggc aacgcgatga gctgggtcca
     1741 gatgatcacg ctggtcgccg gcgcggtagt gctgatgtgg ctcggcgagc tgatcaccga
     1801 gaagggcatc ggcaatggca tcagcttcat catcttcgcg ggcatcgtga gccgcgctcc
     1861 ggcgggcgtc ctcgccacgc tccagcagcc caacccgcgc gacatcatcc tcttcgcgat
     1921 cctcgccatc gccgcggtcg gcgtgatcat ctacatccag gaaggccagc gccggatccc
     1981 gatccagtac gccagtcgcg ttcgcggccg gcgcatgtac cagggcggcc agacgttcct
     2041 gcccctgcga gtgaacctcg cgggcgtcat cccgatcatc ttcgcgatca gcatcctggc
     2101 cttcccgggc cagctcgccg cctacttcca gttctcggag gtcgagggcg tccgcaacgt
     2161 cgccacggcg atcggcggct tcctggcgcc gacgacgatc cagtactcga tcatctactt
     2221 cctgctgacc gtcggattca cgtacttcta cacggcattc acgttcaagc ccgacgagac
     2281 cgccgagcag ctgcgcaaga acggcgggtt catcccgggc atccggcccg gccggcccac
     2341 acaggactac cttgcccggg tcgtgacccg gatcaccttc gccggcgcgc tgttcctggc
     2401 gatcgtcgcc gtgtccccat cgctcctcgc cctcgcgctg cccgacctcg cgacgctcgg
     2461 cctgggcggc accagcctgc tcatcgtggt gtccgtcgtg gtcgagacga tgaagcagat
     2521 cgaggcgca
//
LOCUS       DATBSW010000262         6650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_800913, whole genome shotgun sequence.
ACCESSION   DATBSW010000262 DATBSW010000000
VERSION     DATBSW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800913"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VIF63_07430"
     CDS             complement(<1..261)
                     /locus_tag="VIF63_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8239251.1"
                     /translation="MTPTLRRRIQSAETLFGAWDSLGSPGSAELLGRAGLDWVVVDLE
                     HGNATEATLLAHLTAIEGTGAAALVRPQSGERLRIGRALDMGA"
     gene            279..977
                     /locus_tag="VIF63_07435"
     CDS             279..977
                     /locus_tag="VIF63_07435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8239252.1"
                     /translation="MTATTSGLRDLWPLYGLRLTTGDLVLRYPTEAELPAFVDVIERG
                     IHPPDEMPFGIAWTDVPPARRNVDSYGWWMGSRARWSPENWTLTFGVWEGDTPLGFQD
                     LRGPEFPVNRTVATGSWIGREFQGRGIGKQMRQAVLALAFDHLGAQVAETEAFIDNPA
                     SNRVSLGVGYEPNGFGQLAPRGVTRPTQRFRLTLEGWRARPRPAVVVEGLEGCWHMFG
                     LDPAPPAPSESGPA"
     gene            complement(980..4225)
                     /locus_tag="VIF63_07440"
     CDS             complement(980..4225)
                     /locus_tag="VIF63_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8239253.1"
                     /translation="MAEERRLVTVLFADVVGSTALGESLDPEDVRDLLRRLFAVATDA
                     VERHGGRVEKFIGDAIMAVFGVPIAHDDDAARALAAALDLRDRVRSDPALSSRVPLRL
                     GVNGGEVIASREADAGILITGDAVNMAARLEQAASQWSILVGERTVRAVGERFQFGPA
                     IEVEAKGKALPIVARELLGVASSRPRRRTTRIVGRESDLDQLELVARRTFEERRPYLV
                     SIVAPAGVGKSRLLEEFLAHLDPGVKVAVAQCLPYGQRLTYWPMRAILLSIVGLPDDS
                     SPEDVRAALGSWLREANEPDPDRTAELLAATVGAAEMDGDRLAVFAAWRRLVELAGER
                     APQVVVIEDLHWSSDSLLDLVESMLLPRADVPLLMIALARPELLDRRPGWGGGRRNAI
                     SISLEPLPSRAVAALVADLLDNPAQEIVDAVVDRAEGNPFYAGEIVRSLADRLGPVPD
                     AAAVPGAIAALPDTVHATVLARIDALGPAARRVVQLGAVLGRSFEPRAIPVVEPALDT
                     ATVESAIDDLLDRDLVRQAPPYAVTFRHILIREVAYNTLPRVERARIHGAAGAWLEGQ
                     ANATGRADELAELVAFHLREAVTLSSLLADPVPEALKASAVSWLRRAAEAAAAGAATV
                     EAARHLNAAIELAPKSGQPDLYERLGQIWSGGDQGAEAFERAWELVRELDLGPEAELR
                     TLGQAMTVRARWTGSIGGRLSNEEATQRLQRIEELLATAETDAARLHGELALGFRQML
                     NNFSEAEELDHDAQWAARAMATARRIGQPDLISQAYDALGAVEIGRDRMARVLELAKE
                     RHLIEDRLSTGERADGWIVQTWSETILGDLAAAAESADRARAGLVPGRASSFVLGATC
                     WRVVALHALGRWDEALVEASRAERAWQESELRAPWYAINGFLAALAVARGRDDAVGAA
                     HWAGLVSRIEDGSDAEVRTRRLMTYVTEDLDALARDVIAPFRVFAGRFDYVHLTLALL
                     ADRRHPVDAAVLDDLIAYVDERGLRLVTAQALRLRGLVGRDRESLERALELFEAMAAA
                     PSVARLQTELGMLKGDAAMVDRGLDGLSAIGDVDQAHRVAAERRAAALSPS"
     gene            complement(4228..4698)
                     /locus_tag="VIF63_07445"
     CDS             complement(4228..4698)
                     /locus_tag="VIF63_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8239254.1"
                     /translation="MTTLSAIFDHNQWANERLLEACRGLTPEQLAMSVVGTYGALGET
                     LAHIASGEVFYTQLLSGWKPSGAWQQDDPFPGVDPLLEVVRETGSRLLAEAESLGPDA
                     TIERDPGEMIPATVILVQAINHATEHRAHANTILTQLGLMPPALDGWEHGGYPG"
     gene            4751..5719
                     /locus_tag="VIF63_07450"
     CDS             4751..5719
                     /locus_tag="VIF63_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8239255.1"
                     /translation="MPRAVALGIGLTIVSAFAFGSGALFAKPVYAEGVGWHVLMAWRF
                     LVGASLAMAWLLINPGTRAALLRMRRRDVGAAIALGVLYTGNSGTYFAGLETVSASLA
                     ALIVYIYPAIVAVISLQVGQPLRGRRAWGALALALTGVALAVGNIQNAAPPPLSGLVL
                     IALSPVIYSVWIVLAARLSGERRAGAGVDSGTGAVEPMVAGALMLSSTAVTYWVSALA
                     LGQPVLPAQIPPGAWFGLVGIGVVSTFIAVLAFYAGAHRIGAARASIVSTVEPIWTIS
                     LASLLLAESLGPLQLLGGALILSGVVVAQTGGGSGATATELRIADE"
     gene            5804..6358
                     /locus_tag="VIF63_07455"
     CDS             5804..6358
                     /locus_tag="VIF63_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8239256.1"
                     /translation="MDRDLVEQAQRGDREAFGVLARTRADRLFAIAQRILRDVDRAED
                     AMQQALVIAWRELPRLRDPDRFDAWLNRLLVNACYAEARQRRDWSANIRLLPVDGPQV
                     EDGTRQVADRDELERGFRRLPPEQRAILVLHHYLGLNPIEVAETLDIPTGTARSRLHY
                     AHRAMRAALEAVARSSAIAGKGIS"
BASE COUNT          922 a         2403 c         2363 g          962 t
ORIGIN      
        1 ggcgcccatg tcgagcgcgc ggccgatgcg gagccgctcg ccggattgcg gacgcacgag
       61 cgcggccgcc ccggtgccct cgatcgcggt gaggtgcgcg agcagcgtgg cttcggtcgc
      121 attgccgtgc tcgaggtcga cgacgaccca gtcgaggcct gcgcggccga ggagctcggc
      181 ggagccgggc gagccgaggc tgtcccaggc gccgaagagg gtctcggcgg attggatgcg
      241 gcggcggagg gtgggggtca cgtccgtatc atgccgggat gacagcgacg acgtcgggcc
      301 tgcgcgacct gtggccgctc tacggcctgc gcctgacgac cggcgacctc gtgctgcgct
      361 acccgaccga ggccgagctc ccggcattcg tcgatgtcat cgagcgtggg atccacccgc
      421 ccgacgagat gccgttcggc atcgcctgga cggacgtgcc gcccgcccgc cggaacgtcg
      481 attcgtacgg gtggtggatg ggctcgcgcg ctcgctggtc acccgagaat tggaccctga
      541 cgttcggggt ctgggagggt gacacgccgc tcggcttcca ggacctgcgc gggcccgagt
      601 tccccgtcaa tcgaacggtc gcgaccggat cgtggatcgg gcgtgagttc cagggccgcg
      661 gcatcggcaa gcagatgcgc caggccgtgc tcgcgctcgc gttcgaccac ctcggggcgc
      721 aggtggcgga gaccgaggcg ttcatcgaca acccggcgtc gaacagggtg agcctcggcg
      781 ttggctacga gccgaatggc ttcggtcagc tcgcgccgcg cggcgtcacc cgcccgaccc
      841 agcgcttccg cctgacgctc gaaggctggc gggctcggcc gaggccggcg gtcgtcgtcg
      901 aggggctcga gggttgctgg cacatgttcg ggctcgatcc cgcgcccccg gcgccgtcgg
      961 agtccggtcc ggcctgacct cagctcggtg acagggcggc ggcgcgtcgc tccgcggcga
     1021 ccctgtgggc ctggtcgaca tccccgatcg cgctgagccc atccagaccg cgatcgacca
     1081 ttgccgcatc gcccttcagc atcccgagct cggtctgcag ccgagcgaca gacggcgccg
     1141 cggccatggc ctcgaagagc tccagggcgc gctcgaggct ctcgcgatca cgtccaacga
     1201 ggccgcggag tcggagcgcc tgcgcggtga cgagtcgcag gccgcgttcg tcgacatacg
     1261 cgatgaggtc gtcgaggacc gctgcatcga caggatgtcg ccggtcggcg agcagggcga
     1321 gcgtcaggtg gacgtagtca aagcggccgg cgaagacccg gaacggcgcg atgacgtcgc
     1381 gagccagcgc gtcgaggtcc tcggtcacgt aggtcatgag ccggcgcgtc cgcacctcgg
     1441 cgtcggaacc gtcctcgatg cgcgagacca ggccggccca gtgcgccgcg ccgaccgcat
     1501 cgtcgcgacc gcgcgccacc gcgagcgccg ccaggaagcc attgatcgcg taccacggcg
     1561 cccggagctc cgactcctgc caggcccgct cggcacggct cgcctcgacg agtgcctcgt
     1621 cccatcggcc cagcgcgtgc agtgccacga cgcgccagca cgtcgcgccg agcacgaacg
     1681 acgaggcccg cccgggcacg agtccggcgc gggccctgtc ggcggactcg gcggcggcgg
     1741 cgagatcgcc gaggatggtc tctgaccacg tctggacgat ccagccgtcg gcccgctctc
     1801 cagtcgacag tcgatcctcg atcaggtggc gctcctttgc cagctcgagc acgcgcgcca
     1861 tccggtcccg tccgatctcg accgcgccga gggcgtcgta ggcctggctg atcaggtctg
     1921 gctggccgat acggcgggcc gtcgccatcg cccgcgcggc ccattgcgcg tcgtggtcca
     1981 gctcctcggc ctcgctgaag ttgttgagca tctggcggaa gccgagcgcc agctcgccgt
     2041 gcagccgagc cgcatccgtc tccgcggttg ccagcagctc ctcgatgcgc tggagccgtt
     2101 gcgtcgcttc ctcgttggag agccggccac cgatcgaccc cgtccagcgg gcccgtactg
     2161 tcattgcctg gcccagtgtc cgtagctcgg cctccgggcc gaggtcgagc tcgcgaacga
     2221 gctcccacgc tcgctcgaac gcttccgctc cttggtctcc gccgctccag atctggccca
     2281 gccgctcgta caggtccggc tgcccggact tcggggcaag ctcgatcgct gcgttcaggt
     2341 ggcgggccgc ctccacggtc gccgcgcctg cggcagcggc ctcggcggca cgccgcagcc
     2401 agctcaccgc actcgccttg agggcctccg gaaccgggtc ggcgaggagc gatgacagcg
     2461 tgaccgcttc acggagatgg aacgcgacga gctcggccag ctcgtcggcc cggccggtgg
     2521 catttgcctg gccctcgagc caggcaccgg cggcgccgtg gatccgcgcc cgctcgacgc
     2581 gcggcagcgt gttgtaggca acctcgcgaa tgaggatgtg gcggaatgtg acggcatacg
     2641 gcggcgcttg gcgcaccagg tcccggtcga gcaggtcgtc gatcgccgat tccacggtcg
     2701 cggtgtcgag cgccggctcg accaccggga tcgcgcgcgg ctcgaacgag cgcccgagca
     2761 ccgcaccgag ctggacgacg cgccgcgcgg caggtccgag cgcatcgatg cgcgcgagca
     2821 cggtcgcgtg gacggtgtcc ggcagcgcgg cgatcgcacc gggcacggca gcggcatccg
     2881 gcaccggccc gagccggtcg gcgagcgacc gaacgatctc gccggcatag aacgggttgc
     2941 cctcggcacg atccaccact gcgtcgacga tctcctgcgc cggattgtcg aggaggtccg
     3001 ccacgagggc ggcgacggcg cgggatggca ggggctcgag ggagatcgag atcgcgttgc
     3061 gccggccgcc gccccagccc ggccggcgat cgagcagctc ggggcgggcc agggcaatca
     3121 tcaggagcgg cacgtcggcg cgcggcagga gcatcgattc gacgaggtcg agcaggctgt
     3181 cgctcgacca gtgcaggtcc tcgatcacga ccacctgggg cgcccgttca ccggccagct
     3241 cgacgagacg ccgccacgca gcgaagaccg ccaatcgatc gccgtccatc tcggcggcac
     3301 cgacggtggc ggcaaggagc tccgcggtcc gatcgggatc gggctcgttg gcctcgcgca
     3361 gccagctccc gagggcggcg cggacgtcct cgggcgagga gtcatccggc aacccgacga
     3421 ttgacagcag gatcgcgcgc atcggccagt aggtcaggcg ctggccatag ggcaggcatt
     3481 gcgcgaccgc gaccttgacg ccggggtcga ggtgggcgag gaactcctcg agcagccgcg
     3541 acttcccgac cccggccggc gcgacgatgc tcaccaggta cggccggcgc tcctcgaacg
     3601 tgcgccgggc gacgagctcg agctggtcga gatcggactc gcgacccacg atccgggtcg
     3661 tgcgccggcg cgggcggctg ctcgcaacgc cgaggagctc gcgagccacg atcggcaggg
     3721 ctttgccttt cgcctcgact tcgatggccg ggccgaactg gaaccgctcg ccgaccgccc
     3781 gcacggtccg ctccccgacg aggatcgacc actggctcgc ggcctgctcg aggcgcgccg
     3841 ccatgttgac cgcgtcgccg gtaatgagga tcccggcgtc cgcctcgcgc gaggcgatca
     3901 cctcgccgcc gttgaccccc aaccggaggg ggacgcggct cgagagcgcc gggtcggaac
     3961 ggacccggtc gcgcaaatcc agcgccgcgg ccagggcccg ggcggcgtcg tcgtcgtggg
     4021 cgatcgggac gccgaacacg gccatgatcg cgtcaccgat gaacttctcc acgcgcccgc
     4081 cgtggcgctc cacggcgtcg gtcgcgaccg cgaacaggcg gcgaagcagg tcgcggacgt
     4141 cctccgggtc gagcgactcg ccgagcgcgg tcgagccgac gacgtcggcg aagaggacgg
     4201 tgacgagccg gcgctcctcg gccatgccta gccggggtac ccgccgtgtt cccagccgtc
     4261 gagggcgggc ggcatgagcc cgagctgggt caggatcgtg ttggcatgcg cgcgatgctc
     4321 ggtcgcgtga ttgattgcct ggacgagaat gacggtcgcc gggatcatct cgcccggatc
     4381 gcgctcgatg gtggcgtcgg ggccaaggga ttcggcctcc gccaggaggc gcgaaccggt
     4441 ctcgcgcacg acctcgagca gcgggtcgac ccccgggaac ggatcgtcct gctgccacgc
     4501 gccagatggc ttccaaccgc tgagcagctg cgtgtagaag acctcgccgg acgcgatgtg
     4561 ggcaagcgtt tccccgagcg ctccgtacgt gcccacgacg gacatcgcga gctgctccgg
     4621 cgtcaggccg cggcacgcct ccagcagtcg ctcgttggcc cattggttgt ggtcgaagat
     4681 cgcactgagt gtcgtcatgg cgcggagtat ggcggctcgc gggccgcaga tcgcctgcta
     4741 gcctcccccg atgcctcgcg ccgttgcgct cggaatcggg ctcacgatcg tgtccgcgtt
     4801 cgccttcggg tcgggcgcgc tgttcgccaa gcccgtctac gcggagggcg tcggctggca
     4861 cgtgctgatg gcctggcggt tcctcgtcgg cgcctcgctg gcgatggcgt ggctgctcat
     4921 caatccgggg acgcgggccg cgctgctccg catgcgtcgg cgggacgtgg gcgccgcgat
     4981 cgccctcggc gtgctgtaca cgggcaactc aggcacgtac ttcgcgggcc tcgagacggt
     5041 gtccgcgtcg ctcgcggcgc tgatcgtcta catctacccc gcgatcgtcg cggtgatctc
     5101 actccaggtc ggccagcccc tgcgcggccg ccgagcgtgg ggcgcactcg cgctggccct
     5161 cacgggagtg gccctggcgg ttggcaacat ccagaacgct gcgccgccgc ccctcagcgg
     5221 cctcgtcctg atcgctctct cgccggtcat ctactcggtc tggatcgtgc tcgcggcgcg
     5281 gctctcgggt gagcgtcgcg ccggcgccgg ggtcgattcc gggaccggcg cggtcgagcc
     5341 gatggtcgcc ggcgccctga tgctctcctc aaccgcggtg acctactggg tcagtgcgct
     5401 cgccctcggc cagccggtcc tgccggccca gatcccgccg ggcgcgtggt tcgggctcgt
     5461 cggcatcggc gtcgtctcga cgtttatcgc ggtcctcgcc ttctatgccg gcgcgcatcg
     5521 gatcggagcg gcccgcgcct cgatcgtgag cacggtcgaa ccgatctgga ccatcagtct
     5581 cgcgagcctc ttgctcgcgg agtcgctcgg accgctccag ctcctcggcg gcgcgttgat
     5641 cctcagcggc gtcgtagtcg cgcagacggg cggcggctcg ggcgcgaccg ccacggagct
     5701 ccgaatcgcc gatgaatgaa ccgaaaattg aacgctgcgc cgaggccctg actcagtagg
     5761 ctcggaacaa cgcccccaac gggcgcaccc tctcgaggca gcggtggatc gcgatctcgt
     5821 cgagcaggcc cagcggggcg accgggaggc cttcggcgtc ctggctcgaa cccgtgccga
     5881 ccggctcttt gcgatcgcac aacggatcct ccgcgacgtc gatcgggccg aggatgcgat
     5941 gcagcaggca ctggtgatcg cctggcgcga gctgccccgc ctccgcgatc cggaccggtt
     6001 cgacgcgtgg ctcaaccgcc tccttgtcaa cgcctgctac gccgaggccc gccagcggcg
     6061 ggactggagc gccaacatcc gcctcctgcc ggtcgacgga ccccaggtcg aggacgggac
     6121 gcgccaggtc gccgatcgcg acgagctcga acgtggcttc cggcgactgc cgccggaaca
     6181 gcgggccatc ctcgtcctgc accactacct cgggctcaac ccgatcgagg ttgccgagac
     6241 gctcgacatc ccgaccggga cggcccgatc tcgactccat tacgcccacc gagccatgcg
     6301 tgccgccctc gaggcggtcg cacgatcctc ggcgatcgct ggaaagggca tctcatgagc
     6361 ggccgaaccg agatcgaacg agccctcgac ggattcctcg ccgagggacc ggaaacggtc
     6421 agagaccacg cccttatgcg cgccctcggc gcgatcgacc gaaccaatca gcgacgcggc
     6481 ccgttcgcgt cgtggaggtt ccccgtcatg aacacctcta cccgcctcgc cgcggtagcc
     6541 gtcgtggctg ccattgcgat cggcggcgcc ctctacgtca tcggcccacg cgggggtgtg
     6601 gccagctcgt ccccggcccc gacggcgacg gccatcccga gcccgtcgct
//
LOCUS       DATBSW010000263         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_95669, whole genome shotgun sequence.
ACCESSION   DATBSW010000263 DATBSW010000000
VERSION     DATBSW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95669"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VIF63_07460"
     CDS             <1..191
                     /locus_tag="VIF63_07460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY8239257.1"
                     /translation="SKETEALARKVTEMTGRTVDLRVAVDESLIGGLTVRVGDTLYDA
                     SVRGRLERLRERLVAGAR"
     gene            205..1737
                     /gene="atpA"
                     /locus_tag="VIF63_07465"
     CDS             205..1737
                     /gene="atpA"
                     /locus_tag="VIF63_07465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY8239258.1"
                     /translation="MAIRSDEIVNIIKSAIDTFDADAETRNVGTVVEVGDGIAQIYGL
                     EGALSSELLEFPGGVMGMALNLEEETVGAVILGDARAIKEGDTVKTTGRVVEVPVGQA
                     LLGRVVDPLGKPLDDKGPIETTKTRPVERIAPGVIVRKGVDTPVQTGIKAIDALIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGQGLVCIYVAIGQKLSTVAQTVATLEKYGAM
                     EHTIVVVAGAEDPAPLQYLAPYAGAAFGEEVMEVGVEIDGVLVKDALCVYDDLSKHAW
                     AYREMALLLRRPPGREAYPGDVFYLHSRLLERAARLNDENGGGSLTALPMIETQAGDV
                     SAYIPTNVISITDGQIFLETDLFNAGQRPALNIGISVSRVGSAAQTKAMKNVAGPLKL
                     DLAQYRALAAFAQFASDLDKATRDQLTRGEKLSEVIKQPQYQPLAVEKQVAILFAATT
                     GKLDDIPTPRVKDFEIGFYRFLESEKPAILAEIGKSKALSPENAKALTEALDAFRETF
                     LA"
     gene            2046..>2603
                     /locus_tag="VIF63_07470"
     CDS             2046..>2603
                     /locus_tag="VIF63_07470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HEY8239259.1"
                     /translation="MPSPRDIRRRINAAKNIKQITRAMQFVAASKLRRAQESTLSARP
                     YSEKIDEVLADVAAVLGSEDHPLLADPTGTKRLLILITTDRGLAGPLNTNTIRFVARE
                     ITEHPGDLAVVSVGRKGRDAMRRARVPIEAHFAGFGDKPTFADVLPLARLITQDFLSG
                     EYGRIDLVYSRFVSTLSQRPVMDQLL"
BASE COUNT          432 a          924 c          873 g          374 t
ORIGIN      
        1 ccagcaagga gaccgaggcg ctcgcgcgca aggtcaccga gatgaccggt cgaaccgtcg
       61 accttcgggt cgcggtcgac gaatcgctga tcggcgggct caccgtccgc gtcggcgaca
      121 ccctgtatga cgccagcgtc cgtggccgcc tcgagcggct tcgcgagcgc ctcgtggcgg
      181 gcgcccgcta gctaggagac ccagatggcc attcgctccg acgagatcgt caacatcatc
      241 aagtccgcga tcgacacgtt cgacgccgac gccgagacgc gcaacgtcgg cacggtcgtc
      301 gaggtcggcg acggcatcgc ccagatctac ggcctcgagg gcgcgctgtc ctcggagctg
      361 ctcgagttcc ccggcggggt gatgggcatg gccctcaacc tcgaggagga gacggtcggc
      421 gcggtcatcc tcggcgacgc gcgcgccatc aaggaaggcg acacggtcaa gaccacgggt
      481 cgcgtggtcg aggtgccggt cggccaggcg ctgctcggcc gggtcgtcga cccgctgggc
      541 aagccgctcg acgacaaggg ccccatcgag acgacgaaga ctcgcccggt cgagcgcatc
      601 gccccgggcg tcatcgtccg caagggcgtc gacacgccgg tccagacggg tatcaaggcc
      661 atcgatgccc tcatcccgat cggccgcggc cagcgtgagc tgatcatcgg cgaccgccag
      721 acgggcaaga ccgcgatcgc ggtcgacacg atcatcaacc agaagggcca ggggctcgtc
      781 tgcatctacg tcgccatcgg ccagaagctg tcgacggtcg cccagaccgt ggcgacgctc
      841 gagaagtacg gcgcgatgga gcacacgatc gtcgtcgtcg ccggcgccga ggatcccgcc
      901 ccgctccagt acctcgcccc gtacgcgggc gccgcgttcg gcgaggaagt catggaggtc
      961 ggcgtcgaga tcgacggggt gctcgtcaag gacgcgctgt gcgtctacga cgacctgtcg
     1021 aagcacgcct gggcgtatcg cgagatggcc ctgctgctcc gccgcccgcc gggccgcgag
     1081 gcgtacccgg gcgacgtctt ctacctccac tcgcggctcc tggagcgcgc ggctcggctg
     1141 aacgacgaga acggcggcgg ctcgctcacg gccctcccga tgatcgagac gcaggccggc
     1201 gacgtgtccg catacatccc gacgaacgtc atctcgatca ccgacggcca gatcttcctc
     1261 gagaccgacc tgttcaatgc cggccagcgg cctgcgctga acatcggcat ctccgtgtcg
     1321 cgcgtcggct cggctgccca gaccaaggcc atgaagaacg tcgccggccc gttgaagctc
     1381 gacctcgcgc agtaccgggc cctggccgcg ttcgcgcagt tcgcgtccga cctcgacaag
     1441 gcaacgcgcg accagctgac ccgcggcgag aagctgtccg aggtcatcaa gcagccgcag
     1501 taccagcccc tcgcggtgga gaagcaggtc gcgatcctgt tcgcggcgac gaccggcaag
     1561 ctcgacgaca tcccgacgcc gagggtcaag gacttcgaga tcggcttcta ccggttcctc
     1621 gagtccgaga agcccgcgat cctcgccgag atcggcaagt caaaggccct cagccccgag
     1681 aatgccaagg ctctgaccga ggccctcgac gccttccgcg agaccttcct ggcgtgatgc
     1741 gcacggctgc gccgagttct tcgcccacgg cgggaaatcc atgccaattg ctgcatattc
     1801 cgtggccggg cgtcgatcgc ggggcggggc ctggcgggcc ttggcgggac ggccgtgatc
     1861 gggacggccg ctccagtgac ggccgctatg cagcgattgg cataggtttt cggcgccccg
     1921 ctggaacctc ggtcgcgccc gctcggtcgc actcgctcat gaagctgccc gccgcaacgg
     1981 gtgcccgccc caacggcgtg caacatgccg cgcgcgccgc gcgcgacgcg gcgagggcgt
     2041 gacccatgcc tagcccgcgc gatattcgac gccgcatcaa cgcggcgaag aacatcaagc
     2101 agatcacgcg agcgatgcag ttcgtggcgg cgtcgaagct gcgccgtgcc caggaatcga
     2161 cgttgtcggc ccggccgtac agcgagaaga tcgacgaggt ccttgccgac gtggccgccg
     2221 tcctcgggag cgaggatcac ccgctgctcg ccgacccgac cgggaccaag cgcctgttga
     2281 tcctgatcac gacggaccgc gggcttgccg gcccgctcaa cacgaacacg atccggttcg
     2341 tcgcgcggga gatcaccgag cacccgggcg acctcgcggt tgtcagtgtc gggcgcaaag
     2401 gccgcgacgc gatgcgccgg gcgcgcgtgc ccatcgaggc gcacttcgcg ggcttcggcg
     2461 acaagccgac gtttgccgac gtgctgcccc ttgcccggct catcacccag gacttcctgt
     2521 cgggcgagta cgggcggatc gacctggtct acagccgctt cgtctcgacg ctgtcgcaga
     2581 gaccggtcat ggaccagctc ctg
//
LOCUS       DATBSW010000264         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_95698, whole genome shotgun sequence.
ACCESSION   DATBSW010000264 DATBSW010000000
VERSION     DATBSW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95698"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..946
                     /gene="trpA"
                     /locus_tag="VIF63_07475"
     CDS             113..946
                     /gene="trpA"
                     /locus_tag="VIF63_07475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY8239260.1"
                     /translation="MIGTRDTVGARAIARAFKGAAAEHRTALIPYIVAGYPDAETSYE
                     IALATIDAGADLLEIGLPYSDPLADGATLQRASQAALKAGATLDGSIKLVRRISKARP
                     GVPLVPMCYANQVIGGGDGAVAASRLAGAGASGIIVADLTPDEGAPFEAAACPVGLAV
                     VYLIAPTTPADRRQRIADRSAGFLYCVSLVGVTGARTSLPSDVAKLVREVVAQSPVPV
                     GVGFGVSKPAHVRALARAGAQGVIVASALVDALGPDGRDIAALGSLVRSLREAGATLA
                     A"
     gene            943..1602
                     /locus_tag="VIF63_07480"
     CDS             943..1602
                     /locus_tag="VIF63_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239261.1"
                     /translation="MSPANRDRLAVGLEGTGKKVFASALDWPGWSRSAKTPEAAIEGL
                     LAYAARYRPVVALTGLTLGSTFDVEVDEQVDGGSGTEFGVPSAIHAADARPATAAEAQ
                     RLAAIVEAAWTTFDRVAAGAPAELRKGPRGGGRDRDKMVGHVIEADWYYARELGLKFK
                     APKPDDRPAVEAARREVLAILRQPSDGSPIFRRWPLRYAARRIAWHALDHVWEMEDRS
                     D"
     gene            complement(1631..2518)
                     /locus_tag="VIF63_07485"
     CDS             complement(1631..2518)
                     /locus_tag="VIF63_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8239262.1"
                     /translation="MSATGRDGSVVLADGRTLEYWDGGDPGGRPMIYQPGTPVSRVFG
                     RWGHEAAVDAGVRLIALNRPGYGGSTTSDGVPSLLSVGRDTAELAAILGLDGFAVFGS
                     SGGGPFATATAIAAPDAVRALGVVGGIGPWRLVDPPEANREDRVCLALLDEGDAKGCW
                     ACLAVDVRRSQVSPTQAADNLLATDASPLARDPAYRDLWIENWAIVQENVDGYIADNI
                     AWGGPWDVDPRDVVAPTLLLFGSVDAHCSAEVHGRWYADRIAGSELVVVPTEGHFDAI
                     DGHWPEVLAGLLRIWRAPK"
     gene            complement(2528..>2791)
                     /locus_tag="VIF63_07490"
     CDS             complement(2528..>2791)
                     /locus_tag="VIF63_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239263.1"
                     /translation="ATATQIRLGRLLGRADPPRFAHHPLVRRPDGSKLSKADGATAVG
                     ELLDDGKRREALFGEAAFRVGLIAAAGSLTFAKALVLVADATG"
BASE COUNT          389 a         1061 c          964 g          377 t
ORIGIN      
        1 atcccgacga tgcgctcgtc ctgctcgggt tctcgggacg cggggacaag gacctcgggc
       61 cgttcggcaa gtggcgcgaa cgggcgggcc gcgcatgatc gcgccgccgc cgatgatcgg
      121 gacgcgcgac acggtcggcg cgcgcgccat cgcccgagcc ttcaagggcg cagccgcaga
      181 gcaccgtacg gccctgattc cgtacatcgt cgcgggctat cccgacgccg aaacctcgta
      241 cgagatcgcc ctcgcgacga tcgacgccgg cgccgacctc ctcgagatcg gcctgccgta
      301 ctcggatccc ctggcggacg gcgccacgct ccaacgcgct tcgcaggcgg cgctcaaggc
      361 gggtgcgacc ctcgacggct cgatcaagct cgtccgccga atctcgaagg cacggcccgg
      421 cgttccgctc gtgccgatgt gctacgcgaa ccaggtgatc ggcggcggcg acggcgccgt
      481 cgctgcttcg aggctcgcag gcgccggcgc atcggggatc atcgtcgccg acctcacgcc
      541 ggatgagggc gcgccgttcg aggccgcggc gtgcccggtc gggctggcgg ttgtctacct
      601 gatcgcaccc acgacccccg ccgatcggcg gcagcgcatc gccgatcgca gcgcgggttt
      661 cctctactgc gtctcgctgg tcggcgtcac cggcgcccgc acgagcctcc cgtcggacgt
      721 tgccaagctc gttcgagagg tcgtggcgca gtcgcccgtg cccgttgggg tcgggttcgg
      781 ggtcagcaag cccgcccacg ttcgtgccct ggcgagggcc ggcgcgcagg gtgtcatcgt
      841 cgcctcggcg ctcgtcgatg cgctcgggcc cgacgggcgg gacatcgcag cgctcgggag
      901 cctcgtgcgg tcgctccggg aggccggcgc taccctcgcc gcgtgagccc agcgaaccga
      961 gaccgcctcg cggtcggcct cgaaggcacc ggcaagaagg tcttcgcctc ggcgctggac
     1021 tggcccggtt ggtcgcgctc cgcaaagacg ccggaagccg cgatcgaagg gctcctcgcc
     1081 tacgccgcgc gctatcgacc cgtcgtcgcc ctgaccggcc tgaccctcgg atcgacgttc
     1141 gacgtcgagg tcgatgagca ggtcgacggc ggctccggca ccgagttcgg cgtcccgagc
     1201 gcgatccatg cggcagacgc ccgacccgca acggccgccg aggctcagcg gctagccgcg
     1261 atcgtcgagg cggcctggac gacattcgac cgtgtcgccg ccggcgcgcc cgccgagctc
     1321 cgcaagggcc cgcgcggcgg tggccgcgac cgcgacaaga tggtcggcca cgtgatcgag
     1381 gccgactggt actacgcccg cgaactcggc ctgaaattca aggccccgaa acccgatgac
     1441 cgacccgccg tcgaggccgc ccgtcgcgag gtgctcgcca tcctccgcca accctcggac
     1501 ggctcgccga tcttccgccg gtggccgctg cgctacgccg ctcgccgcat cgcatggcac
     1561 gccctcgacc acgtatggga gatggaagac cgttccgatt agggtgcgct gcaagtccgc
     1621 ccgccggctg ctacttcggc gctcgccaga tccggagcag acccgccaac acctcgggcc
     1681 aatgcccatc gatcgcgtcg aagtggccct ccgtcgggac cacgacgagc tccgaacccg
     1741 caatccgatc cgcgtaccag cgaccgtgga cctcggccga gcaatgggcg tcgacactgc
     1801 cgaacagcag gagcgtcggc gcgacgacgt cgcgtggatc cacatcccag gggccgcccc
     1861 aggcaatgtt gtcggcgatg tagccgtcca cgttctcctg gacgatcgcc cagttctcga
     1921 tccacaggtc gcggtacgcg ggatctcggg ccagcgggct cgcgtcggtc gcgaggaggt
     1981 tgtccgccgc ctgcgtcggg ctgacctggc tccggcgcac gtcgacggcg aggcaggccc
     2041 agcaaccctt ggcgtcgcct tcgtccagga gcgccaggca aacccggtcc tctcggttgg
     2101 cttcgggcgg gtcgaccagc cgccacggtc cgatcccgcc cacgacccca agcgcgcgaa
     2161 ccgcgtccgg tgcggcgatg gccgtcgccg ttgcgaacgg gccgcccccc gacgacccga
     2221 agaccgcgaa tccgtccagt ccgaggatcg ccgccagctc cgcggtgtcc cgcccgacgg
     2281 acagcaggct cggcaccccg tcgctcgtgg tcgagccgcc gtacccgggg cggttcagcg
     2341 cgatcagccg gaccccggcg tcgaccgcgg cctcgtgacc ccagcgcccg aacacgcgac
     2401 tcacgggcgt accgggctgg tagatcatcg gccgcccgcc ggggtcgccg ccgtcccagt
     2461 actcgagggt ccggccgtcg gcgaggacga cgctgccgtc gcgtccggtc gcgctcatga
     2521 cccgatccta gcccgtggca tcggcaacga ggacgagcgc cttggcgaac gtcagcgagc
     2581 cggccgccgc gatgaggccc acgcggaatg ccgcctcacc gaacaaggcc tcgcgccgct
     2641 tcccgtcgtc gagcagctcg ccgaccgcag tcgcgccgtc ggccttgctg agcttcgagc
     2701 cgtccgggcg gcggacgagc gggtggtggg cgaaccgcgg cggatccgca cgcccgagga
     2761 gccggccgag ccggatctgc gtcgccgtgg c
//
LOCUS       DATBSW010000265         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1224239, whole genome shotgun sequence.
ACCESSION   DATBSW010000265 DATBSW010000000
VERSION     DATBSW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224239"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VIF63_07495"
     CDS             complement(<1..656)
                     /locus_tag="VIF63_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239264.1"
                     /translation="MARIKRLIILSTAAMLGGIGASVGLLADPVTVSEAFVTNQNGDS
                     LTIVSLADMKPLAEIAIGGKPAGIAMSPDGRHAYLTSPDSKELVIVDAAARQVSSRLK
                     VGEGPLGIAAHPTSGRVYVADWFTHKISVIDPSVPKVVAEIAVGESPSGLAVSPDGKL
                     LLSADRDSDAVSIIDAQSNTRIGTVSVGKRPFGITIDAEGARAYTANVASDDVSVIDI
                     A"
     gene            complement(673..1119)
                     /locus_tag="VIF63_07500"
     CDS             complement(673..1119)
                     /locus_tag="VIF63_07500"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8239265.1"
                     /translation="MTETVEINAPADKVWAVIGNFQDMSWHPAIAKLEGSGGNDANAT
                     RTLTLTSGGTIDEKLLSNDPAKKIYKYEITKVDPKVLPVNNYSASLSVKGEGDKSTVE
                     WKGAFYRGFMNNDPPPELNDEAAVKAVKGVYRGGLDALKTKVEAGG"
     gene            1373..>2091
                     /locus_tag="VIF63_07505"
     CDS             1373..>2091
                     /locus_tag="VIF63_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239266.1"
                     /translation="MTAQAEGDRPGEHAASHTYDNVTCPFCGLLCDDLQVTRTGSTLK
                     VSNGCPRATAGFQRPLPPAKPLVDGREVSYEEAVKAAAGLVRGAKLPLYGGMASDVAG
                     ARAVLSIADRTCGVLDHALSEGQYRNFRVVQTSGWIMSTLTEVRNRADLLLIVGTDVH
                     KLHGRFFDRIVTNPHTMFESLANKRTVVFIGEGLDASAAVGPRIEEVITLPVAKDRIA
                     EVLAAISARVRGFHVPAPEAS"
BASE COUNT          340 a          717 c          657 g          377 t
ORIGIN      
        1 gcgatgtcga tgacgctcac gtcgtcgctg gcgacgttgg ccgtgtaggc gcgcgcgccc
       61 tcggcgtcga tggtaatccc gaacggacgc ttgcccaccg acaccgtacc aatccgcgtg
      121 ttcgactgag cgtcgatgat cgataccgcg tcgctgtccc ggtcggcgct cagcagcagc
      181 ttgccgtcgg gactcaccgc gagacccgaa ggcgactcgc cgacggcgat ctcggcgacc
      241 accttcggca ccgacggatc gatgacggag attttgtggg tgaaccagtc ggcaacgtac
      301 acgcggccgc tcgtcggatg ggcggcgata cccaacggac cttcgccgac cttcaagcgg
      361 ctggagacct ggcgcgccgc tgcgtcgacg atgacgagct ctttgctgtc aggcgacgtc
      421 aggtaagcgt gcctgccgtc gggcgacatg gcgatacccg ccggcttgcc accaatggcg
      481 atctccgcca gtggcttcat gtcggccagc gacacgatcg tcagcgagtc accattctga
      541 ttggtgacaa aggcctcgct caccgtcacc ggatcggcca acagtcccac cgacgcgccg
      601 atgccgccca gcatcgccgc cgtcgatagg atgatcagcc gcttgatgcg cgccatcggt
      661 tgagggcgga cgtcagccgc ccgcttcgac tttcgtcttc agagcatcga ggccgccgcg
      721 ataaacgccc ttcacggcct tcaccgccgc ctcgtcgttc agttccggcg ggggatcgtt
      781 gttcatgaac ccgcggtaga aggcgccttt ccactcgacc gtcgatttgt cgccctcgcc
      841 cttgacgctg aggctcgcgg aatagttgtt caccggcagc accttcgggt cgaccttggt
      901 gatctcgtat ttgtagatct tcttggccgg atcgttggaa agaagcttct cgtcgatcgt
      961 cccgcccgag gtcagggtca gggtgcgggt cgcgttggcg tcgttgcctc cggacccctc
     1021 gagcttggca atggccgggt gccagctcat gtcctggaag ttgccgatca cggcccagac
     1081 cttgtcggcc ggcgcattga tttcgacggt ctccgtcacc ttctgacgcg agggaccgtg
     1141 agcggacgca agggtcggga acagggccag cacagccagc cccagcgcaa cagctctcac
     1201 cattaggctt cctccggtat cggtttttta gcgcgcctcg ggcgagacca tagtggcgcc
     1261 aaaaggcctt cgcaatcggc gccatccccg gcaaaccgcc gcattcgcgc cgctgttgtt
     1321 ttgccagggc gtccgtgctt acttgcgccg cgcgcgaagg agactgcacc gtatgaccgc
     1381 acaagccgag ggtgatcgtc cgggcgagca cgccgcttcc cacacctacg acaacgtcac
     1441 ctgccccttc tgcggactac tgtgcgacga tctgcaagtc acccgcaccg gatcgaccct
     1501 caaggtcagt aacggctgcc cgcgggcaac ggcgggtttc cagcgcccgc tgccgccggc
     1561 caaaccgctc gtcgacggcc gcgaggtcag ctatgaggag gcggtcaagg cagccgcggg
     1621 cctcgtccgc ggagccaagc tgccgttgta cggcggcatg gccagcgacg tcgccggggc
     1681 gcgcgccgtc ctgtcgatcg ccgaccgcac ctgcggcgtg ctcgatcatg cgctcagcga
     1741 gggccagtat cgcaacttcc gcgtagtgca gacgagcggc tggatcatgt cgacgctcac
     1801 tgaggtgcgc aatcgcgccg acctgctgct catcgtcggc accgatgtgc acaagctgca
     1861 cggccgcttc ttcgatcgca tcgtcaccaa tccgcacacg atgttcgaaa gcctcgccaa
     1921 caagcgcacg gtggtgttca tcggcgaggg cctcgacgcc tcggcggcgg tcggcccgcg
     1981 catcgaggag gtgatcacgc tacccgtcgc caaggatcgc atcgccgagg ttctcgccgc
     2041 gatatcggcg cgtgtgcgcg gcttccacgt tcccgcgccc gaggcgtcgg a
//
LOCUS       DATBSW010000266         5887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_378375, whole genome shotgun sequence.
ACCESSION   DATBSW010000266 DATBSW010000000
VERSION     DATBSW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378375"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VIF63_07510"
     CDS             <1..616
                     /locus_tag="VIF63_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8239267.1"
                     /translation="GTSLSLRGLRALVARAPRAVVDGAGVVRVLDVGTGAADIPVAIV
                     RAKGPWRAVRVTAIDSRPEVIEAARSINPELADDRIELAVADGLALPHPDGAFDVAHA
                     SLVLHHLEPDAAVGFLRELSRVAGIGVVVNDLGRGRLAWLGAWLILHAMTRNPYTLHD
                     GPLSVRRAYTRAEARDLLAGAGLVPTFEASGLAGHRWVIAAVRG"
     gene            613..1842
                     /locus_tag="VIF63_07515"
     CDS             613..1842
                     /locus_tag="VIF63_07515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8239268.1"
                     /translation="MKAGAVDVAIVGGGPAGAVTAMLLAQRGLDVVLLERAPAWRWRA
                     CGVFTSPATVKALQRVGVSATDLARVCRPIPAMRVETPRGTSFALSYGGTGSLADSPV
                     GLDRSVLDPMFLAMAGSAGADVRTGVTVESVRLADASDAPVAQLTMSGGRQLEASVVV
                     GADGLRSIVAAAAGVARPPALGRRTGLTFHVPDGVSRTEGVVHDLPMDARMVVIEHGY
                     VGLAPVPGNRLNVGIVLGASWLPRLRQEGAARVARSILDRIHPPGTADATRVLDHVAG
                     ASPLGTGARRRAGVGWLVVGDAAGFLDPFTGEGLHRAIVSAEMAVEAIMRSRVAGASA
                     DLRGYDAAMRSRFAMKDLVSRITQAFLGQPALFEYAARRLAARSELRETMGLVIGDLA
                     PASRALDPRFLARLLAP"
     gene            1853..2362
                     /locus_tag="VIF63_07520"
     CDS             1853..2362
                     /locus_tag="VIF63_07520"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8239269.1"
                     /translation="MTSTIAIDIGAPASLLFDLARNVERWPDLLPHYRFVRVRERHAD
                     SAVTAEFVAVRSFVPAIGLGIPVAWRSRSWSEAQTLRLRFVHQGGATDGMDVTWRIDP
                     TPTGCRVTIEHDFRPRFPLWAGLIDRFFVRPIAGRTLAAFKAMAEAIAASGAADVRSD
                     PSPAANTPA"
     gene            2359..3636
                     /locus_tag="VIF63_07525"
     CDS             2359..3636
                     /locus_tag="VIF63_07525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY8239270.1"
                     /translation="MTRRRVWITGLGLVTPIGTGRDPFWAGVHAARSTVRRIDRFDPS
                     PFRSQVAAQIDDFDPLDHMDARTARQLDRFSQFGLAAGQLALADAGLAVESAGTGKGA
                     GADRDRFGVYLGSALGGIAYAELQHERYIERGIKAVAPNLALAVFGGAAPANLGIALD
                     LRGPILSTANSCASGAVALGEALRAIRDGTIDAAIAGGAEVPLSPLAFGAFDIIRALS
                     SGSNDTPERAARPFDADRDGFVMGEAVGLLVLEAEEIAERRGATPYAELMGYGSTSDA
                     HHMVQPRPDGREAARAVGIALADAGVGADELDYVNAHASSTPLGDTAEARALALALGA
                     SAARMPVSGTKALYGHPLGASSALEASICALAIRDGWAPGTANLETPDPSVTEHLPGL
                     LAEGRPGRYDRVLSTSFGFGGLNAALVFGAVGE"
     gene            complement(3757..4737)
                     /gene="deoC"
                     /locus_tag="VIF63_07530"
     CDS             complement(3757..4737)
                     /gene="deoC"
                     /locus_tag="VIF63_07530"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY8239271.1"
                     /translation="MAVRQPDTISRERASELVKAWAGGRPGPLGSVLGRQTAVDQVGV
                     ELRVETLKKRSIKKASKLYALDLAIRMMDLTTLEGRDTPGKIRALCAKAMHPQPGDPT
                     IPSVAAICLYPTLIPEAKEALRGSTVKVASVATGFPSGQTFRSIKVAEVREAVAAGAD
                     EVDMVIDRGAFLSGDYATVYDEIVEIKDSCGDAHLKVILETGELGTFDQVRRASILAM
                     AAGADFIKTSTGKIQPAATLPVTLVMLEAIRDFEKATGKIIGMKPAGGIRTAKEAVQY
                     LVVLYETLGPRWMTPDRFRFGASTLLNDVLMQIEKERTGRYQDPDHFTLD"
     gene            4759..5529
                     /locus_tag="VIF63_07535"
     CDS             4759..5529
                     /locus_tag="VIF63_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239272.1"
                     /translation="MGRPSDQLSMRLDTSLPNLPAGLRPMLARPLPEPFDSSAHLFEP
                     IWGGARALALIGPAEAAGTGDVVLVAEDGTSGPAPVELAGLAGRVAARSAVLDGEIVV
                     VDEAGRLDRDELARRFAGEPGRPLSYLAFDLLHLDGKSLLNLALDKRRALLRQVLRPG
                     DEVVAVPSIVSEGRALFEAVSSQGLLGIRARQRESPYLPGIRSRLWRSVAVGGQSHAE
                     PRLIVGAAEVVDALDIEPDRSERVLALIRRLPLEFEGE"
     gene            complement(5536..>5887)
                     /locus_tag="VIF63_07540"
     CDS             complement(5536..>5887)
                     /locus_tag="VIF63_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8239273.1"
                     /translation="AGSLVSRAEDLQVLALPLSLVAIAGYLQAVMALSGGISPLVRIA
                     SYVPFWSPFVMLTRLTVGRVEPWELVLSFGLLILAIPIVGIVAVRVYSAGVLLYGQRP
                     GARQIIGAIVRPPV"
BASE COUNT          743 a         2113 c         2142 g          889 t
ORIGIN      
        1 cggcaccagc ctttcgctcc gagggctgcg tgcgctcgtt gcacgggcgc cgcgagcggt
       61 ggtcgacggc gccggcgtcg tgcgtgtgct cgacgtcggc accggtgccg cggacatccc
      121 ggtcgcgatc gtccgggcga agggcccgtg gcgtgccgtc cgggtcacgg cgatcgacag
      181 ccggcccgag gtgatcgagg cggcgcgctc gatcaacccc gagctggcgg acgaccggat
      241 cgagctcgcc gtcgccgacg gcttggccct gccccatccc gatggcgcgt tcgacgtcgc
      301 gcacgcgtcc ctggtcctcc atcacctcga gcccgacgcg gcggtcgggt tcctgcgcga
      361 gctttctcga gtcgcgggca ttggcgtggt cgtgaacgat ctcgggcgtg ggcggctcgc
      421 ctggcttgga gcgtggctca tcctccatgc gatgacccgc aacccgtaca cgctccacga
      481 cggcccgctc tcggtgcgcc gcgcctacac gcgggccgag gcgcgcgacc tcctcgccgg
      541 ggccggcctc gtcccgacct tcgaggcctc cgggcttgcc ggtcaccggt gggtcatcgc
      601 ggcggtgcga ggatgaaggc aggcgccgtc gacgtggcga tcgtcggtgg cgggcccgcg
      661 ggcgccgtga cggcgatgct gctcgcgcag cgcgggctcg acgtagtcct cctcgagcgc
      721 gcgccggcgt ggcgctggcg cgcgtgcggc gtgttcacgt cgcctgcaac ggtgaaggcg
      781 ctccagcgtg tcggcgtgtc ggcgacggat ctcgctcgcg tctgtcgacc gatccccgcg
      841 atgcgcgtcg agaccccgcg aggaacgagc ttcgcactgt cctacggcgg gaccggcagc
      901 ctcgccgatt cgcccgttgg cctcgaccgc tccgtgctcg atccaatgtt cctggcgatg
      961 gccggctcgg cgggcgccga cgtccggacc ggtgtcaccg tcgagtccgt gcgcctcgcg
     1021 gacgcgtccg acgcgccagt cgcccagctc acgatgagtg gcgggcggca gctcgaagcg
     1081 agcgtcgtcg tcggcgccga cggccttcgt tccatcgtcg ccgcggccgc cggcgtggct
     1141 cgaccgccgg cgctcggccg acggaccggc ctcaccttcc acgtcccgga cggcgtgtcc
     1201 cgaacggagg gagtggtcca cgacctgccg atggatgcgc gcatggtcgt gatcgagcac
     1261 ggctatgtcg gcctggcgcc cgtgcccggc aatcgactca acgtcgggat cgtgctcggg
     1321 gcgtcgtggc tgccgagact gcggcaggag ggcgccgcgc gcgtcgctcg atcgatcctc
     1381 gatcggatcc atccgcccgg taccgcggac gccacccggg tcctcgatca cgtcgccggc
     1441 gcctcgccgc tagggaccgg ggcgcgccga cgagcggggg tcggctggct ggtcgtcggc
     1501 gacgcggcgg gcttcctcga cccatttacc ggtgagggcc ttcatcgggc gatcgtgtcg
     1561 gcggagatgg ccgtcgaagc gatcatgcgg tcccgcgtcg ccggcgcctc ggcggacctt
     1621 cggggctacg acgcggcgat gcgatcgagg ttcgcgatga aggacctggt gagccgcatt
     1681 acccaggcct tcctgggcca gccggccctg ttcgagtacg cggcgcggcg cctggcggct
     1741 cgaagcgagc tccgtgagac gatgggcctc gtgatcggcg atctcgctcc ggcctcgcgg
     1801 gcgctcgatc cccgatttct cgccaggctt ctcgcaccat gagggcggac gcgtgacgtc
     1861 gacgatcgcg atcgacatcg gcgccccggc gagcctcctc ttcgacctcg cgcggaacgt
     1921 ggaacgctgg cctgacctcc tcccccacta ccgattcgtt cgcgtgcgcg agcgccacgc
     1981 agacagcgcg gtgacggccg agttcgtcgc ggtgcgttcg ttcgtgccgg ccatcggcct
     2041 gggcatcccg gtcgcgtggc gatcccgatc gtggtccgag gcccagacgc tgcgcctgcg
     2101 cttcgtccac cagggcggcg cgaccgatgg catggacgtc acctggcgga tcgacccgac
     2161 gccgactggc tgtcgggtga cgatcgagca cgacttcagg cctcgattcc cgctgtgggc
     2221 cggcctcatt gatcggttct tcgtgcgccc gatcgccggc cgcacgctcg ccgcgttcaa
     2281 ggcgatggcg gaggccatcg cggcgtccgg cgccgccgac gttcgatccg acccctcgcc
     2341 cgcggcgaac acaccggcat gacgcgacgt cgggtctgga tcaccggcct cgggctcgtc
     2401 acgccgatcg gcaccggccg ggacccgttc tgggcgggcg tccacgcggc gcggtcgacc
     2461 gtgcgccgca tcgaccggtt cgacccctcg ccgttccgct cccaggtcgc ggcccagatc
     2521 gacgacttcg acccgctcga ccacatggat gcgcggacgg cccggcagct cgatcggttc
     2581 agccagttcg gcctcgccgc cgggcagctg gcgctggccg atgccgggct cgcggtcgag
     2641 agcgccggca cgggcaaggg cgccggcgcg gatcgcgatc ggttcggcgt ctacctcggc
     2701 agcgcactcg gcggcatcgc gtatgccgag ctccagcacg agcgctacat cgagcgcggc
     2761 atcaaggccg tcgcgccgaa cctcgccctg gcggtgttcg gtggcgcggc gccggcgaac
     2821 ctcgggatcg ccctcgacct tcgcgggccg atcctgtcga ccgccaactc gtgcgcgtcc
     2881 ggcgccgtcg cgctgggcga ggccctgcgc gcaattcgtg acggcacgat cgatgccgcg
     2941 atcgctggcg gcgccgaggt gccgctgagc ccgctcgcat tcggcgcgtt cgacatcatc
     3001 cgcgcactct cgtccggctc gaacgacacg ccggagcggg ccgcacgacc cttcgacgcc
     3061 gatcgcgacg ggttcgtcat gggcgaggcc gtcggcctgc tcgtgctcga ggccgaggag
     3121 atcgcggaac gccgcggcgc gacgccgtac gcggagctga tgggctacgg ctctacatca
     3181 gacgcccacc acatggtcca gccccgaccc gatggccgcg aggcggcgcg cgcggttggc
     3241 attgcgcttg ccgatgccgg cgtcggcgcg gacgagctcg actacgtgaa tgcccacgcc
     3301 tcctcgacgc cgctcggcga caccgccgag gcacgtgcgc tggccctcgc gctcggtgcg
     3361 tcggcggcgc gcatgccggt gagtggcacg aaggcgctgt acgggcaccc gctcggcgcc
     3421 tcgagcgcgc tggaagcgtc gatctgcgcc ctggcgatcc gcgacggttg ggcgccgggc
     3481 acggcgaacc tcgagacgcc cgatccgtcg gtgaccgagc acctgccggg cctgctggcc
     3541 gaaggccgcc ccggccgtta cgaccgcgtg ctgtcgacct cgttcggctt cggcggactc
     3601 aacgcggcgc tggtgttcgg agcggttgga gagtagacgg gcgatccggc tggtccagcc
     3661 gaaggcggag gcgagcacga tcgcgagcgc actgcagtgt gcgtgagcgg gcgcgcgcag
     3721 ccgacaacgc ccggatggac cgagccgggg cgcccgtcag tccagggtga agtggtccgg
     3781 gtcctggtag cgccccgtgc gttctttctc gatctgcatc aggacatcgt tgagcagcgt
     3841 cgacgcgccg aagcggaagc gatcgggggt catccagcgc gggccgagtg tctcgtacag
     3901 cacgacgagg tactggacgg cctccttggc ggtgcggatg ccgccggccg gcttcatgcc
     3961 gatgatcttg ccggtcgcct tctcgaagtc ccggatcgcc tcgagcatca ccagcgtgac
     4021 cggcagcgtc gcggccggct ggatcttgcc ggtcgaggtc ttgatgaagt cggcaccggc
     4081 ggccatcgcc aggatcgagg cgcggcggac ctggtcgaac gtgcccagct cgccggtctc
     4141 gaggatgacc ttgagatgcg cgtcgccgca cgagtccttg atctcgacga tctcgtcgta
     4201 gaccgtcgcg tagtcgcccg acaggaacgc gccgcggtcg atgaccatgt cgacctcgtc
     4261 ggccccggcc gcgaccgcct cgcgcacctc ggcgaccttg atcgagcgga aggtctggcc
     4321 tgacgggaag ccggtcgcga cgctggccac cttgacggtc gacccgcgca gggcttcctt
     4381 cgcctcgggg atgagggtcg ggtagaggca gatcgcggcg acggacggga tcgtcgggtc
     4441 gcccggctgc gggtgcatcg ccttcgcgca cagcgcccgg atcttgcccg gcgtgtcgcg
     4501 gccctcgagc gtcgtgaggt ccatcatccg gatcgcgaga tcgagcgcgt agagcttcga
     4561 cgccttcttg atcgagcgct tcttgagcgt ctcgacccgc agctcgaccc cgacctggtc
     4621 gaccgcggtc tgtcgcccga gcacgctgcc cagtggcccg gggcgcccgc ctgcccacgc
     4681 cttcaccagc tcgctggcgc gctcgcggga gatcgtgtcg ggctgccgta ccgccatggg
     4741 agtgggagga tacaccgcat gggacgcccc tccgaccagc tctcgatgcg cctcgacacg
     4801 tccctgccga acctgccggc aggccttcgg cccatgctcg cgcggccgct gccggagccg
     4861 ttcgactcgt ccgcgcacct gttcgagccg atctggggtg gggcgcgggc gctggcgttg
     4921 atcggccccg cagaggcagc aggcaccgga gatgtggtgc tcgtcgccga ggacggcacg
     4981 tccgggcccg cgcccgtgga gctcgccggg ctcgccggcc gggttgccgc ccgatcggcg
     5041 gtcctcgatg gcgagatcgt ggtcgtcgac gaggcgggcc ggctggaccg ggacgagctt
     5101 gcgcggcggt tcgcgggcga gccggggcgg cccttgtcgt atctcgcctt cgatctgctc
     5161 cacctcgacg gcaagtccct cctgaatctc gcgctggaca agcgccgggc gctgcttcga
     5221 caggtgctcc ggccaggcga cgaggtcgtg gccgtgccgt cgatcgtgtc cgaggggcgg
     5281 gcattgttcg aggccgtctc gagccagggc ctgctcggca tccgcgctcg ccagcgcgag
     5341 agcccctacc tgcccggcat tcgcagccgg ctctggcgga gcgtggcggt cggtgggcag
     5401 tcccacgccg agccgcgact gatcgtgggc gcggccgagg tcgtcgatgc cctggacatc
     5461 gagcccgacc gctcggagcg cgtcctcgcg ctgatcaggc gcctgcccct cgagttcgag
     5521 ggcgagtagc ccgcctcaga cgggcgggcg gacgatcgcg ccgatgatct gacgggcgcc
     5581 gggccgctgc ccgtacaaca ggacgcctgc cgagtacacg cggacggcga cgatgccgac
     5641 gatcgggatc gcgaggatga gcaggccgaa cgacagcacc agctcccacg gctcgacccg
     5701 gccgaccgtc aggcgggtga gcatcacgaa cgggctccag aacggcacgt acgacgcgat
     5761 ccgaacgagc gggctgatgc ccccggacag ggccatgacc gcctggaggt agccggcgat
     5821 cgccacgagg ctcaggggca gggcgaggac ctggaggtcc tccgctcggc tgacgagcga
     5881 gccggcg
//
LOCUS       DATBSW010000267         6878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_942597, whole genome shotgun sequence.
ACCESSION   DATBSW010000267 DATBSW010000000
VERSION     DATBSW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942597"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VIF63_07545"
     CDS             complement(<1..214)
                     /locus_tag="VIF63_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239274.1"
                     /translation="MNWRRWPVAFRRLIGLLALFSGVALLLNIIGNISLGLALAGTTL
                     VLAGGIAVALRGQDAESRRWTLCTAAV"
     gene            complement(211..1701)
                     /locus_tag="VIF63_07550"
     CDS             complement(211..1701)
                     /locus_tag="VIF63_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239275.1"
                     /translation="MAGSDLRNVRQSGKPWWSWPALVLDALRPGLAEQAARDVAWLAR
                     APVGIRSLALPVGVVAIPLLFAATRAFDTVGHTPVVVTLRIQDIYTESIPYMVIGASI
                     GILAPTLGVLFLIGHMVGDLIAAFIQPKELSPLPTAILGRLVSFWVLYLLVVELPIAV
                     HEVVASVKPRLKGAAARLGPVVIGVAAATFLAWIWGVGATLFIRPVFTWSDLRFPTSN
                     AGWALLYGSDLFAYAVGAISVATLGLRYVIRPVANEPPVVEAEAQRPGLRSLFFGVVV
                     PILLFSSVINTALDAVVLVVAVIAARPISSIVLRRFALARPLAVIPRPVRLMGGVVLS
                     IAVGYVIASVMGVSQVSPFFTMIVAMAASFILVRILLDADDFASADIPAPSLAATVGS
                     ALAMGLVIWLFSAGWAFADNIAGQTDGWGTAAAAAGAAGGAGGLAAMSAKKNNQGKKP
                     NPPPWYIPDSMGEFFGYDGPPKPPVNPKKQPDWTKPKDPKPGDPVY"
     gene            1805..3091
                     /locus_tag="VIF63_07555"
     CDS             1805..3091
                     /locus_tag="VIF63_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239276.1"
                     /translation="MTTMEAPPPAVVEPPDHVDILLPQGSRILHIGPPKTGTTALQAA
                     FHLGRTQTERQGVHYASHGRHAMSAVLAGIEQPSPWDVERRPPSKLPWNRLVADIRGS
                     KARRVVLSSEFFADATPEAIKRVVTEIGSEDAQIVVTLRPLARIMPSQWQQFVQNQLT
                     VAFDDWVDGVLNRPRGKMTPSFWLRHRHDELVARWVDVVGSERLTVVALDDNDRDMVL
                     RVFERLTGLAVGTLVTEPDLSNRSMTVPEIETVRAFNKSFRDEKLPAPLYSRVMRFGA
                     AAYMRNRQPGPDEARIELPDWSVEPIANLAREMMASIQATGVRIVGNLDLMTQVPAGR
                     APTQEPVAVSPTVAASTAMGVLVASGLARGSLPIIANESEGIEGDERPRRAPRPVQEP
                     PEVLRLSTLQLAVVIFRRGKAALRDRLGTLVFWRRR"
     gene            complement(3088..3648)
                     /gene="cysC"
                     /locus_tag="VIF63_07560"
     CDS             complement(3088..3648)
                     /gene="cysC"
                     /locus_tag="VIF63_07560"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY8239277.1"
                     /translation="MSGGGGGGASGGAVVFLTGLSGAGKSTIAEALAADLEDAGRTVS
                     IIDGDELRRLSAHLGFDTASREANVRHAAELAEEWAREGQIAIAALIAPFERSRREAR
                     EIVERSAPFMLVWIRTPLAVAEARDPKGLYKRVRAGEIRDFTGIDSPYEEPVDADLVI
                     DTTTTEVADAVALIRLGLEERLGPMG"
     gene            complement(3645..4547)
                     /locus_tag="VIF63_07565"
     CDS             complement(3645..4547)
                     /locus_tag="VIF63_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8239278.1"
                     /translation="MTLDLPLAIGGLLVGVIVGLTGMGGGALMTPMLVFFFHADPLTA
                     ISSDLVASFVMKPAGALVHLRRKTVNLGLVKLLCIGSVPSAFVGAWLISLQPRGAELD
                     ETLKVLLGLALLVAAGGLVARALFQMWQNALPLGEGAVRPSRPEVILKPLPTILLGAA
                     AGLVVGITSVGSGSIVIVVLLLIYPGLKASALVGTDLTQAIPLVGAAALGHLLFGSFS
                     PAITVSLLLGAIPGAFIGAQISSRAPGGLIRRALAVLLLASGLRLLGLSTELVLAVAV
                     AALVGGSLLWIGIRRTVKAGRRAA"
     gene            4606..5508
                     /gene="cysD"
                     /locus_tag="VIF63_07570"
     CDS             4606..5508
                     /gene="cysD"
                     /locus_tag="VIF63_07570"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY8239279.1"
                     /translation="MKSHVLPHLRALEAESVFIFREVAAEMTRPCLLFSGGKDSTVML
                     HLARKAFAPAPIPFPIMHVDTGLNFPEVLAYRDKWVEQMSLKLIVASVPDAIRRGLVH
                     EEPNGSRNRIQTPVLLEAAEQNGFDALFGGARRDEEKARAKERVFSFRDEFGQWDPKN
                     QRPELWSLYNGRVQPGQSIRVFPLSNWTELDIWTYIAAEKVEIANLYFAAEREVVRRG
                     DMLYLVNEFTPLRDGDTSEKRMVRFRTIGDANLTAAVESQADTLEKIVDEISAVRVTE
                     RGATRGDDRVSETSMEDRKREGYF"
     gene            5651..>6878
                     /locus_tag="VIF63_07575"
     CDS             5651..>6878
                     /locus_tag="VIF63_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8239280.1"
                     /translation="MPADLLRFATAGSVDDGKSTLIGRLLFDSKTLFIDQLAAVERVS
                     KERGDDYTDLALLTDGLRAEREQGITIDVAYRYFATTKRRFIIADSPGHIQYTRNMVT
                     GASTADVAVLLLDARKGITEQSRRHAFLSTLLGVPHLVLAVNKMDLVDWSEDAFEAIR
                     EEFTDFASRLRVRDLTFIPVSALLGDNVVTKSKRMDWYDGLPLLRHLERLYVASDRNL
                     VDVRFPVQYVIRPQSKTVIDYRGYAGTVASGILKPGDPVLVLPSGLETTIETIETADG
                     PVDEAFPPMAVTITLADHVDVSRGDMLCRPHNIPTIAQEIDAQVCWMDESAPMTEGGK
                     YGIKHTTRWAKALVREIAYRIDVNTLHRHEGVEGLALNEIGRVRLRVTQPLFVDPYSE
                     NRQTGSFILVDERSNKT"
BASE COUNT         1160 a         2395 c         2393 g          930 t
ORIGIN      
        1 cgacagcggc cgtgcacagc gtccatcggc ggctctcggc gtcctggccg cgaagcgcga
       61 cggcgatgcc gcccgcgagg acgagcgtgg ttcccgccag cgcgaggccc aggctgatgt
      121 ttccgatgat gttgagaagc agcgcgaccc cggagaagag ggcgaggagc ccgatcagcc
      181 ggcgaaacgc gaccggccag cgccgccaat tcaatagacg gggtcgcccg gtttcgggtc
      241 ctttggcttc gtccagtccg gctgcttctt gggattgacc ggcggcttgg gcgggccgtc
      301 gtagccgaag aactcgccca tcgagtcggg gatgtaccac ggcggcgggt tcggcttctt
      361 gccctggttg ttcttcttgg ccgacatggc cgccaggccg cctgcgccgc ccgccgcgcc
      421 cgcggctgcc gcagccgtgc cccacccatc ggtctggccc gcgatgttgt cggcgaacgc
      481 ccagccggcg gagaacagcc agatgaccag gcccatcgcc agcgccgagc cgaccgtggc
      541 ggccagggaa ggcgccggga tgtcagcgga ggcgaagtcg tcggcgtcga gcaggatgcg
      601 caccaggatg aagctggcgg ccatggcgac gatcatcgtg aagaaggggc tcacctggga
      661 gacgcccatc acgctggcaa tcacgtagcc aaccgcaatc gagaggacca cgccgcccat
      721 caaccggacc ggtcgcggga tgacggcgag gggtcgagcc agcgcgaagc gtcgcaggac
      781 gatcgagctg atcggtcgag cggcgatgac cgcgactacg agcaccacag cgtcaagggc
      841 cgtattgatg accgacgaga acagcaggat gggcacgacc acgccgaaga acagcgaccg
      901 caggccggga cgttgggcct ccgcctcgac gacaggcggc tcgtttgcca ccgggcgtat
      961 gacatagcgc agccccaacg tggcgacgct gatcgcccca acggcatagg caaacaggtc
     1021 cgatccgtac aggagggccc agccggcatt cgacgtgggg aagcgcaggt cgctccaggt
     1081 gaagaccggc cggatgaaga gcgtggcgcc gacgccccag atccaggcca ggaaggtcgc
     1141 cgcggcaacg ccgatcacga cgggccccaa gcgcgccgcc gcgcccttga gccgcggctt
     1201 cacggacgca acaacctcgt ggaccgcgat cggcagctcg acgacgagca ggtacaggac
     1261 ccagaaggag acgaggcgtc cgaggatcgc ggtcgggagg ggcgacagct ccttcggctg
     1321 gatgaacgcc gcgatcaggt ctccgaccat gtgcccgatc aggaacagga ccccgagggt
     1381 gggcgccagg atcccgatcg acgccccgat gaccatgtac gggatcgact cggtgtagat
     1441 gtcctggatc cggagggtga cgacgaccgg cgtgtggccc acggtgtcga aagctcgtgt
     1501 cgccgcgaac agcagcggga tcgcaacgac cccgacgggc agcgcgaggg agcgaatccc
     1561 gaccggggct cgcgccagcc aggcgacgtc gcgggcggcc tgctccgcca ggccgggccg
     1621 caacgcgtcg aggaccaggg ccggccacga ccaccacggc tttcctgact gcctgacgtt
     1681 gcgcagatcc gatccagcca cggacgtcac cccctcgttt cgatgctagc gcccgcgatg
     1741 gtcgaaggtc agcgatgttg cggtcgcgcc cgcgacccgt ccggacttgg gacaatggac
     1801 gccgatgacg acgatggagg cgcccccgcc cgccgtagtg gagccgccgg accacgtcga
     1861 catcctgctg ccgcagggtt cgcggatcct gcacatcggc ccgcccaaga ccgggaccac
     1921 ggcgctccag gccgcgttcc acctgggtcg gacgcagacc gagcggcagg gcgtccatta
     1981 cgccagccat gggcgccatg cgatgagcgc ggtcctcgca gggatcgaac agccctcgcc
     2041 gtgggacgtc gagcggcgcc cgccgtcgaa gctgccctgg aaccgactcg tcgccgacat
     2101 ccgcggctcg aaggcgcgac gcgtggtcct gagcagcgaa ttcttcgccg acgcgacgcc
     2161 cgaggcgatc aagcgggtcg tgaccgagat cggctcggag gacgcgcaga tcgtcgtcac
     2221 gctccgtccg ctcgcccgga tcatgccctc gcaatggcag cagttcgtcc agaaccagct
     2281 gacggtcgcg ttcgacgact gggtcgacgg cgtgctcaat cggccgcgcg gcaagatgac
     2341 gccgtcgttc tggttgcgtc accgccacga cgagctcgtc gcgcgctggg tggatgtcgt
     2401 cggatccgag cggttgaccg tcgtcgccct cgacgacaac gatcgcgaca tggtgctgcg
     2461 cgtcttcgag cgcctgaccg ggctcgcggt cggaacgctc gtcaccgagc cggacctctc
     2521 gaatcgatcg atgacggtgc ccgagatcga gacagtgcgg gcattcaaca agtcgttccg
     2581 ggacgagaag ctgcccgcgc ccctgtacag ccgggtgatg cgcttcggcg ccgcggcgta
     2641 catgcgcaac cggcagcccg ggcctgatga ggcgcgcatc gagctcccgg actggtccgt
     2701 cgagcccatc gcgaatctcg ctcgcgagat gatggcctcg atccaggcca cgggcgtgcg
     2761 gatcgtgggc aatctcgacc tcatgacgca ggtcccggcc gggcgagcgc cgacccagga
     2821 gccggtcgcg gtgagcccca ccgttgccgc gtcgaccgcg atgggcgtgc tcgtcgcgag
     2881 cgggctcgcg cgcggctcgc tcccgatcat cgccaacgaa tcggagggca tcgagggcga
     2941 cgagcgtccg cgacgggcgc cccgaccggt ccaggagccg ccggaggtgc tgcgcctctc
     3001 cacgttgcag ctcgcggtgg tgatcttccg tcgcggcaaa gccgcgctcc gggaccggct
     3061 cgggaccctc gtgttctggc ggcgccgcta gcccataggc ccgaggcgct cctcgaggcc
     3121 gaggcgaatc agcgcgacgg catcggccac ctcggttgtc gtcgtgtcga tgacgaggtc
     3181 ggcgtcgacc ggctcctcgt agggcgagtc gatgcccgtg aagtcgcgaa tctcgccggc
     3241 gcggacgcgc ttgtagaggc ccttcggatc tcgagcctcg gcgaccgcga gcggcgtccg
     3301 gatccagacg agcatgaacg gcgccgaacg ctcgacgatc tcgcgcgcct cgcggcggga
     3361 ccgctcgaac ggggcgatga gcgcggcgat ggcgatctgg ccctcgcgag cccattcctc
     3421 ggccagctcg gcggcgtggc gaacgttcgc ctcgcgcgac gcggtgtcga agccgaggtg
     3481 cgccgacagt cgtcgcagct cgtcgccatc gatgatcgag accgtgcgac ccgcatcctc
     3541 gagatcggcg gccagcgcct cggcgatcgt cgacttgccc gcaccggaca ggcccgtgag
     3601 gaacacgacc gcgccgccgc tcgcgccgcc gccgccgccc ccgctcatgc ggcgcgccgc
     3661 ccggccttga ccgtgcgccg gatcccgatc cagaggaggc taccgccgac gagcgcggcc
     3721 accgccacgg ccaggacgag ctccgtgctc aggccgagca gccgcagccc ggaggcgagg
     3781 aggagcacgg cgagcgcacg acggatgagg ccgccgggcg ccctgctcga gatctgcgcc
     3841 ccgatgaatg cccccgggat tgccccgagg aggagcgaca cggtgatcgc cggcgagaag
     3901 ctgccgaaca gcaggtgacc gagcgcggca gccccgacca gcgggatcgc ctgggtcagg
     3961 tccgtgccga cgagtgccga cgccttcagg cccggataga tcagcaacag cacgacgatc
     4021 acgatggacc ccgagcccac cgacgtgatg ccgacgacca gccccgcggc ggcgccgagc
     4081 aggatcgtgg gcagcggctt gaggatcacc tccggtcgcg agggccggac cgcgccttca
     4141 ccgaggggca gggcgttctg ccacatctgg aacagcgctc gggcgacgag gccaccggcg
     4201 gcaacgagca aggccagccc gaggagcacc ttcagcgtct cgtcgagctc cgcgccccgg
     4261 ggctgcaggc tgatcagcca cgcgccgacg aacgccgagg gcaccgaccc gatgcacagc
     4321 agcttgacga ggccgaggtt gacggtcttg cgccggaggt ggaccagcgc accggcaggc
     4381 ttcatgacga agctcgcgac gagatcgctc gagatcgcgg tcaggggatc ggcgtggaag
     4441 aagaacacga gcatcggggt catgagcgcg ccgccgccca tgcccgtcag gccgacgatg
     4501 acgccgacga gcaggccgcc gattgccagc ggcaggtcga gcgtcacccg gcgatgatac
     4561 cgtcgtcaac ctgccagcct gccccgtcga ggtgaccccg accccgtgaa gtcgcacgtc
     4621 ctgccgcacc tgcgcgcgct cgaggcggag agcgtcttca tcttccgcga ggtcgccgcc
     4681 gagatgaccc gcccgtgcct cctcttctcg ggcggcaagg actccacggt catgctgcac
     4741 ctggcgagga aggcattcgc accggcgccg atcccgttcc cgatcatgca cgtcgacacc
     4801 gggttgaact tccccgaggt cctcgcgtac cgcgacaaat gggtcgagca gatgtcgctc
     4861 aagctcatcg tcgcctcggt gccggacgcg atcaggcgcg ggctcgtcca cgaggagccg
     4921 aacggctcgc gcaaccgcat ccagacaccg gtgctcctcg aggcggccga gcagaacggc
     4981 tttgacgcgt tgttcggcgg cgcccgccgc gacgaggaga aggcccgggc gaaggagcgc
     5041 gtgttctcgt tccgcgacga gttcggccag tgggacccca agaaccaacg tcccgagctg
     5101 tggagcctgt acaacggccg ggtgcagccc ggccagtcga tccgggtgtt cccgctctcg
     5161 aactggaccg agctcgacat ctggacctac atcgccgccg agaaggtcga gatcgcgaac
     5221 ctgtacttcg ccgccgagcg cgaggtcgtc cggcgcggcg acatgctcta tctcgtcaac
     5281 gagttcacgc ccctccgcga cggcgacacc tcggagaagc ggatggtccg gttccggacg
     5341 atcggcgacg cgaacctgac cgcggcggtc gagtcccagg cggacacgct cgagaagatc
     5401 gtcgacgaga tcagtgcggt gcgcgtcacc gagcgcggcg cgacccgcgg cgacgatcgc
     5461 gtttcggaga cgtcgatgga agaccgcaag cgcgagggtt acttctgatg ttcgcgaagg
     5521 ccgcgcccat gcagccgtct cgcggcgttg gccgctgcgc gcgggcgctc acgcacgctc
     5581 aacgtgcgct cgcgctcgtg ctcgcgtccg ccttgcgatc cggccgcctg agcgcggcgg
     5641 gcaggcactg atgcccgcgg acctgctgcg gttcgcgact gccggctcgg tcgacgacgg
     5701 caagtcgacc ctcatcgggc ggctgctctt cgactcgaag acactgttca tcgaccagct
     5761 cgccgcggtc gagcgggtgt cgaaggagcg cggcgacgac tacacggacc tcgcgctgct
     5821 gaccgacggc ctccgcgccg agcgcgagca ggggatcacg atcgatgtcg cctaccgcta
     5881 cttcgccacg acgaagcggc ggttcatcat cgcggactcc ccgggccaca tccagtacac
     5941 gcggaacatg gtcaccggcg cgtcgacggc cgacgtcgcg gtgctcctgc tcgacgcgcg
     6001 aaagggcatc accgaacaga gtcgccgcca cgcgttcctg tcgacgctgc tcggcgtgcc
     6061 gcacctggtc ctcgcggtca acaagatgga cctggtcgac tggtcggaag acgcgttcga
     6121 ggcgatccgc gaggagttca ccgacttcgc ctcgcgcctg cgggtccgcg acctgacctt
     6181 catcccggtg tccgcgctgc tcggcgacaa cgtcgtgacg aagtcgaagc ggatggactg
     6241 gtacgacggc ctgccactgc tgcgccatct cgagcgcctc tacgtcgcga gcgaccgcaa
     6301 cctcgtcgac gtgcggttcc cggtgcagta cgtgatccgg ccgcagtcga agacggtgat
     6361 cgactaccgc ggctacgccg gcacggtcgc ctcgggcatc ctgaagccgg gcgatcccgt
     6421 gctggtgctg ccgagcggcc tcgagacgac gatcgagacg atcgagactg ccgacgggcc
     6481 ggtcgacgag gcgttcccgc cgatggccgt cacgatcacg ctcgccgacc acgtcgacgt
     6541 gagtcggggc gacatgctgt gccggccgca caacatcccg acgatcgccc aggagatcga
     6601 cgcccaggtg tgctggatgg acgagagcgc accgatgacc gagggcggga agtacggcat
     6661 caagcacacg acccggtggg cgaaggcgct cgtccgggag atcgcctatc ggatcgacgt
     6721 gaacacgctc caccgccacg agggcgtcga gggcctcgcg ctcaacgaga tcggccgggt
     6781 gcggttgcgg gtcacgcagc cgctgttcgt cgatccctac tctgagaacc ggcagaccgg
     6841 ctcgttcatc ctggtggacg agcggtcgaa caagaccg
//
LOCUS       DATBSW010000268         5408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_766040, whole genome shotgun sequence.
ACCESSION   DATBSW010000268 DATBSW010000000
VERSION     DATBSW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766040"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VIF63_07580"
     CDS             complement(<1..974)
                     /locus_tag="VIF63_07580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239281.1"
                     /translation="MNREVATPVRVELRPFDPAVDYEPIAELTTTTNVADGVDWALTS
                     ASIQAEVAPGGYWDPERDCRVALVDGERVGFIRVSSKVRDGERVIHRFDVWTRPEWRR
                     RGVGRTLLAWAEERSRAMQVAGSIGEPGMPNFMSGGVNMAQPEVAAAAEALGYERIRY
                     SFLMRRPLDLPIDDAPLPDGLEFRSVREADHRAIFDGNEEAFEDHWEHAERTEDDFRI
                     LFAEPALDTSLWAVAWDGDQVAGVSMNWIDAEENAQLGTNAGWLGSVSVRRPWRKRGV
                     GAAVITASLRTFKERGMDDARLGVDAENPTGALALYTRLGFTPYQTFG"
     gene            complement(992..1972)
                     /locus_tag="VIF63_07585"
     CDS             complement(992..1972)
                     /locus_tag="VIF63_07585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239282.1"
                     /translation="MSATTSAVDLRPFDPASDFPAITELIRDVSTQTGAEWFPTVEAL
                     SIEWSRARNFEPVRDCVVATDAGRVVGLARVSWREREGGVVHRIELWVRPDRMREGIG
                     RRLLAWGETRARASVLDGSGGPAELPHLFGGATDLGREAGTAFAAGAGYSPIRYHYEM
                     RRDLSAPIPDAPVPDGLEIRPVEPEHHRAIWLADAEAFRDHWDASAIHEDDFVRFFAH
                     PDIDTALWQVAWDGDEIAGLVLNAIYREENAQIGVDIGWVDGVATRRPWRGRGVASAL
                     LSRSLVLLRERGMTVAALGVDTENPSGALGLYERFGFAPVRTWAFYRKPF"
     gene            complement(1969..3069)
                     /gene="rsgA"
                     /locus_tag="VIF63_07590"
     CDS             complement(1969..3069)
                     /gene="rsgA"
                     /locus_tag="VIF63_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY8239283.1"
                     /translation="MTIDALPALGWNDRFAALLAHEAPGLAAGRILSEERGVYVVASA
                     TGEGPASPSGRLRHDSELDPTAAWPAVGDWVALEPFTADQVGNEHRLIQRVLPRHTAV
                     VRQAPTDRGMPTQVLAANVDVVFVVTSVNEEFNVRRLERYVTVAWESGAIPVVLLSKA
                     DLADDLERYRVAAEATAAGVEVIAVSVVTGEGIDAVRGHLGAGRTVVFTGSSGVGKSS
                     IVNALAGEALLDTGGIRFDDARGWHTITRRQLVRLSDGLLIDTPGLRELGVLDGEGLA
                     TAFEDVERAAADCRFSDCRHDSEPGCAVRAALQAGTLDAARFDAYQKLQREAHRATLA
                     TDAIARRAERKKWSAIHKRVGEHMRVKYGADR"
     gene            complement(3066..4052)
                     /locus_tag="VIF63_07595"
     CDS             complement(3066..4052)
                     /locus_tag="VIF63_07595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239284.1"
                     /translation="MSTQIAAQVRLRPYAGESDLAELVRILNAEAEADGIERRASVEQ
                     LRASFAHPRQGFDPARDVTVAELDGRPVAYQMRDWVDTTDGLREYRLEGGVEPAARRR
                     GIGTALLADGERRWCAYAADNPVERPLTFGSWAADSAAGARALYSGAGYSPVRYFFDM
                     SRTGLDDAVAEQLPDGLEIRPITRDMALAVWRADIEAFRDHWGGFDGSDERLEEWLAR
                     PSTDLSLWKIAFDGDEVAGGILNTIDAAENEALGIRRGWLSSVFTRRPWRRRGLAHAL
                     ITHSLVALRDRGMTVASLGVDADNPSGALGIYERNGFKVTYRSTAWRKAFDR"
     gene            complement(4052..4855)
                     /locus_tag="VIF63_07600"
     CDS             complement(4052..4855)
                     /locus_tag="VIF63_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8239285.1"
                     /translation="MTALTHEIKASYAFMERNFFLSRRYWGWELAFLVYSAAGALSIA
                     FIGVQLGNPRLSLTLIVGAIFWNYLSVVFSWIAETIQIERWEGTLEYTFMAPVRRSTH
                     LLGAVAYAMVYGLIHTAAIIVAMVLFFPELSIAHANFVTIGAFMLLGSFSLVGIAMLA
                     AILPLLFVERGMQMAFVMQSVLLLVSGVYYSIDVLPGWMQVISWFSPATYTLNAVRAG
                     LIDGVGLDALWSDIWPLLVMGTLFIPFGLWAFGQAERYAKRTGKLKRVG"
     gene            complement(5121..>5408)
                     /locus_tag="VIF63_07605"
     CDS             complement(5121..>5408)
                     /locus_tag="VIF63_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239286.1"
                     /translation="LDPRSKLDVQTFIEEIRLSHDASIVLTTHDLDEAERLSDEIIVL
                     NDGRIVAQGTPEELKRLVAERHGQAPTMHNVFMTYTGRSLDEDVEEDAEDE"
BASE COUNT          855 a         1889 c         1880 g          784 t
ORIGIN      
        1 atcccgaagg tctgatacgg cgtaaagccg aggcgcgtgt acagcgcgag cgcgccggtc
       61 gggttctccg catcgacccc gagccgggca tcgtccatgc cccgctcctt gaaggtccgc
      121 agggacgcgg tgatcacggc ggcaccgacg ccgcgcttgc gccacggccg gcgcaccgac
      181 acgctcccga gccagcccgc attcgttccg agctgcgcgt tctcctcggc gtcgatccag
      241 ttcatcgaca cgccggcgac ctggtcgcca tcccaggcga ccgcccacag ggacgtgtcg
      301 agggccggct ccgcgaacag gatgcggaaa tcgtcctcgg tgcgctcggc gtgctcccag
      361 tggtcctcga acgcctcctc gttgccatcg aagatggcgc ggtggtcggc ctcgcggacg
      421 gaccggaact ccaggccgtc ggggagtggc gcgtcgtcga tggggaggtc cagcgggcgg
      481 cgcatcagga acgagtagcg aatccgctcg tagccgagcg cctcggccgc cgcagcgacc
      541 tccggctggg ccatgttgac cccacccgac atgaaatttg gcatgcccgg ctcgccaatc
      601 gatcccgcga cctgcatcgc ccgcgagcgc tcctcggccc aggccaacag cgttcggccg
      661 acgccgcgcc ggcgccattc cggacgcgtc cagacgtcga agcgatggat gacgcgctcg
      721 ccgtcgcgga ccttggacga gacgcggatg aatccaacgc gttcgccatc gacaagcgca
      781 acacggcaat cgcgttcggg atcccagtac ccgccgggcg cgacctcggc ctggatcgaa
      841 gcggaggtca gcgcccagtc gacgccgtcg gccacgttcg tcgtggtcgt gagctcggcg
      901 atcggctcgt agtcgacggc cgggtcgaac ggccgaagct cgacgcgaac gggagtggcc
      961 acctcgcggt tcatctcgac gatccccctg ctcagaacgg cttgcggtag aaggcccagg
     1021 tccgaaccgg cgcgaacccg aaccgctcgt aaaggccaag tgcccctgac gggttctcgg
     1081 tgtccacgcc cagcgcggcg acggtcatgc cacgctcgcg caggaggacg agggatcggg
     1141 acaggagcgc gctcgcgacc ccgcgtccgc gccatggccg gcgagtggcc acaccgtcga
     1201 cccagccgat gtccacgccg atctgggcgt tctcctcgcg gtagatcgcg ttcaggacca
     1261 ggcccgcgat ctcgtcgccg tcccacgcaa cctgccacag cgccgtgtcg atgtccgggt
     1321 gggcaaagaa ccggacgaag tcatcctcgt ggatcgcgga cgcgtcccag tgatcacgga
     1381 aggcctcggc gtcggcgagc cagatcgcac gatggtgctc cggctcgacc ggtcgaatct
     1441 cgagcccatc ggggacgggc gcatccggga tcggcgccga caggtcgcgg cgcatctcgt
     1501 agtggtaccg gatcggggag tagccggcgc ccgcggcgaa ggccgtgccg gcctcgcgac
     1561 ccaggtccgt cgcgccaccg aagaggtgcg gcagctcggc cggaccgccg gagccatcaa
     1621 gcaccgatgc ccgtgcccgg gtctcgcccc acgcgagcag gcgccggccg atgccctcgc
     1681 gcatccgatc cggccgcacc cagagctcga tgcgatggac gacgccgccc tcgcgctccc
     1741 gccagctcac gcgcgccagg ccgacgacgc ggccggcatc ggtcgccacg acgcagtcgc
     1801 gaaccggctc gaagtttcgt gcccggctcc actcgatgct cagcgcctcg accgtcggga
     1861 accattcggc gccggtctgc gtgctgacgt cccggatgag ctcggtgatc gcgggaaagt
     1921 cagaggcggg gtcgaaaggc cggaggtcga ccgcggaggt cgtcgcgctc atcggtctgc
     1981 tccgtacttg acgcgcatgt gctcgccgac gcgcttgtgg atcgcgctcc acttctttcg
     2041 ctccgcgcgg cgcgcgatcg cgtccgtcgc gagcgtcgcg cggtgggcct cgcgctggag
     2101 cttctggtag gcgtcgaacc gggcggcgtc gagggtgccg gcctggagcg cggcgcggac
     2161 cgcgcagccc ggctctgaat cgtgtcggca atcggagaag cggcaatccg ccgcggcgcg
     2221 ctcgacgtcc tcgaacgcgg tcgcgaggcc ctcgccatcg aggacgccga gctcgcgcag
     2281 gcccggggtg tcgatgagca ggccgtcgga aaggcgaacg agctggcgtc gcgtgatggt
     2341 gtgccagccg cgagcgtcgt cgaagcgaat gcccccagtg tcgagcaggg cctcgcccgc
     2401 gagggcattg acgatgctcg acttgcccac accggacgag ccggtgaaca cgacggtccg
     2461 ccctgctccg aggtggccgc gcaccgcgtc gatgccctcg ccggtcacga cggacaccgc
     2521 gatcacctcg acgccggcgg cggtcgcctc cgccgcgact cggtagcgct cgaggtcgtc
     2581 ggcgagatcc gccttcgaca gcaacacgac cgggatggcc ccggactccc acgcgaccgt
     2641 gacgtagcgc tcgagccggc ggacgttgaa ctcctcgttc accgaggtca ccacgaagac
     2701 gacatcgacg ttcgcagcga gcacctgggt gggcatgccc cgatcggtcg gcgcctgtcg
     2761 aacgacggcc gtgtggcggg gaagcacgcg ctggatcagc cggtgctcgt tcccgacctg
     2821 gtcggccgtg aacggctcga gggccaccca gtcgccgacc gccggccagg cggccgtggg
     2881 gtcgagctcg ctgtcatggc ggaggcggcc ggacgggctt gccggcccct cgccggtcgc
     2941 cgaggcgacg acatacacgc cgcgttcctc ggagaggatc cgtcccgcgg ccaggccggg
     3001 tgcctcgtgc gcgaggagcg cggcgaagcg atcgttccag ccgagagccg gcagcgcgtc
     3061 gatcgtcatc ggtcgaacgc cttgcgccag gcagtcgatc gataggtgac cttgaagccg
     3121 ttccgctcgt agatgccgag cgcgccgctc gggttgtcgg catcgacgcc gaggctcgcc
     3181 accgtcatgc cgcggtcgcg gagtgcgacc agcgaatggg tgatcagggc atgagccagg
     3241 ccgcgccggc gccatggccg ccgcgtgaac acgctgctga gccagccgcg ccggatgccg
     3301 agcgcctcgt tctccgcggc gtcgatcgtg ttgaggattc cgccggcgac ctcgtcgccg
     3361 tcgaaggcga tcttccacag ggacaggtcg gtcgatggcc gggcgagcca ctcctcgagt
     3421 cgctcgtcgg agccgtcgaa gccaccccag tgatcgcgga acgcctcgat gtctgcgcgc
     3481 cacaccgcga gcgccatgtc gcgcgtgatg ggtcggatct cgagcccatc cgggagctgc
     3541 tcggcgactg cgtcgtcgag gccggtccgc gacatgtcga agaaatagcg gaccggggaa
     3601 tacccggcgc cgctgtacag cgcccgcgcc ccggccgcgc tgtcagccgc ccacgatccg
     3661 aacgtgaggg gccgctcgac ggggttgtcg gcggcgtacg cgcaccagcg gcgctcgccg
     3721 tcggccagca gcgcggtgcc gatgccgcgg cggcgggctg cgggttcgac accaccttcg
     3781 aggcggtact cgcgcaggcc gtcagtggtg tcgacccagt cgcgcatctg ataggccacg
     3841 ggccggccat cgagctcggc caccgtgacg tcccgggcag ggtcaaagcc ctggcgcggg
     3901 tgcgcgaagg acgcccgcag ttgctcgaca gacgcccgcc gctcgatgcc gtcggcctcg
     3961 gcctcggcgt tcaggatccg gaccagctcg gccaggtccg actcgcccgc ataggggcga
     4021 agccgaacct gggccgcgat ctgggtcgac atcagccgac tcgcttgagc ttgccggtcc
     4081 gcttggcgta gcgctcggcc tggccgaacg cccacagccc aaacgggatg aacagggtac
     4141 ccatcacgag gagcggccag atgtcgctcc agagcgcgtc aaggccgacc ccatcgatga
     4201 gcccggcccg caccgcgttc agcgtgtacg tggccggcga aaaccaggag atgacctgca
     4261 tccagcccgg caggacgtcg atcgagtagt agacgccgct gacgagcagc agcaccgact
     4321 gcatcacgaa cgccatctgc atgccgcgct cgacgaagag caacggcagg atcgcggcca
     4381 gcatcgcgat gccgaccagg ctgaacgagc cgaggagcat gaacgcgccg atcgtgacga
     4441 aattcgcgtg cgcgatcgac agctccggga agaacaggac catggcgacg atgatcgcgg
     4501 ccgtatggat gaggccgtag accatggcgt acgcgaccgc acccagcagg tgggtggagc
     4561 gccgcacggg cgccatgaac gtgtactcga gcgtgccctc ccagcgctcg atctggatcg
     4621 tctccgcgat ccagctgaag accaccgaga ggtagttcca gaagatcgcg ccgacgatca
     4681 gggtcaggga cagccggggg ttgcccagct gcacgccgat gaatgcgatc gacagcgccc
     4741 cggcggcgga atacacgagg aacgcaagct cccagcccca gtagcggcgg gacaggaaga
     4801 agttgcgctc catgaaggcg tacgacgcct tgatctcgtg ggtgagtgca gtcatggcgg
     4861 gccttcgcag tggaagggct ggctcggccc tcatcgggac cgagccagcc tgggctgtgg
     4921 ctcggcggca atccgagcgc cgatctcgat gatcgaacgc ccgaacgcgc gccggaccga
     4981 actggacgcg agccgaggct cgtgtcgcgg gacgccgatt cgcttccgaa tctcgcgctc
     5041 gcggtcacgc atctcctgct tcgcgtgcat ctcatgcaac atctggcatc acctcccttc
     5101 gtcgtcgatg tgtgtgtctg tcattcgtct tcggcgtctt cctcgacgtc ctcgtcgagg
     5161 gatcggccgg tgtaggtcat gaacacgttg tgcatcgtcg gtgcctggcc gtggcgctcg
     5221 gcgacgagtc gcttgagctc ctcgggggtg ccctgggcca cgatccgacc gtcgttgagg
     5281 acgatgatct cgtcgctcag gcgctcggcc tcgtcgaggt cgtgggtcgt cagcacgatc
     5341 gatgcgtcgt ggctcagccg gatctcctcg atgaacgtct ggacgtccag cttcgaccgc
     5401 gggtcgag
//
LOCUS       DATBSW010000269         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_731145, whole genome shotgun sequence.
ACCESSION   DATBSW010000269 DATBSW010000000
VERSION     DATBSW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731145"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1205
                     /locus_tag="VIF63_07610"
     CDS             213..1205
                     /locus_tag="VIF63_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY8239287.1"
                     /translation="MARQPIRRSLAGLLAACLAAACSAPAATPGPTTGPTTAPTAIAA
                     TAQPTPTITPTEAPTPVSSPKRDPGTLRQDGKGIDQVWVPGGSFRMGSDLDSATPPSW
                     AVATYHSEQPAHDVTLTRGYWIDATEVTVEAFKAFKDAGGYTVEANWSVEGWKWLQTQ
                     QVSALPNPCLAQEPDEPQVCVTWYEADAYARWRGGRLPTEAEWEFAARSPVSLVYPWG
                     DEYDADLANIDGGTKPVAVGSYPDGASWVGALDMAGNAMEWVADWWSNTYYAESPAMD
                     PPGPASGVIKIEKGGWWGPPAGTGPFVSRASFRLDEDPPTYQDHHIGFRIVTDD"
     gene            1275..1706
                     /locus_tag="VIF63_07615"
     CDS             1275..1706
                     /locus_tag="VIF63_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY8239288.1"
                     /translation="MVDAPTIVSALEDAGYRLTTPRRALAGSIAGRRGHFTAEELLTE
                     SRGRRLGVTRATVFRTLDVLTDLGIVERLDLPNGEHAFVACEAEHHHHIVCSSCGRST
                     WVADPGLERAATAIGEQTGYRIDTHRLELFGLCPECQAADR"
     gene            1703..>2405
                     /locus_tag="VIF63_07620"
     CDS             1703..>2405
                     /locus_tag="VIF63_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY8239289.1"
                     /translation="MNPAALLPFFSTLLGGMAALRLRHRIHVFMALAAGVVLATAVAD
                     LLPEAFELVGEGGALSVGIATVIGFVGFSLVEAFLHQSSFEHVGEGDHEHAQAHDHSL
                     AAAVAARPPSRILGILPPLSLIIHSTLDGLAIGLGFQAGADIGLLVLIAVLAHDFADG
                     MNVVTLALDAARGERLAVVLLLLDALAPLAGAALSVVVTVTPATLGLLLATFAGVFIA
                     IGAGHLLPESQHHDPR"
BASE COUNT          335 a          886 c          814 g          370 t
ORIGIN      
        1 gtcgtcgggg tcggggtgtg gcttgtgctc cagcgccgcc cacgacggta tcgaggggcg
       61 actcgcggcc ggtagccggc gcaccctcgt ttcgacgggc acaatggatg ggaacctgtt
      121 ggacgatttc aacgtctgcc cgttcgaacg ggccccgcga cgttgctcac cggcgtcgac
      181 tgccgaccgc gttcgcttgg aggagcgccc tgatggctcg ccagcccatt cgtcgcagcc
      241 tcgccgggct gctggcggcg tgtctcgccg ctgcctgttc cgcgccggca gccacgccgg
      301 gcccgacgac cgggccgacc accgcgccga cagcgatcgc ggccactgcc caaccgacgc
      361 ccacgatcac gccgacggag gctccgacgc ccgtgtcatc tcccaagcga gatcccggca
      421 ccctgcgcca ggacggcaag ggcatcgacc aggtctgggt gcccggcggc tcgttccgga
      481 tgggctccga cctcgactcg gcgacgccgc cctcgtgggc cgtcgcgacc taccacagcg
      541 agcagccggc gcacgacgtg accctgaccc gtggctactg gatcgatgcc accgaggtca
      601 cggttgaggc gttcaaggcg ttcaaggacg ccggcggata caccgtcgaa gcgaactggt
      661 ccgtcgaggg ctggaaatgg ctccagacgc aacaggtctc ggcgctgccg aacccgtgcc
      721 tggcgcagga acccgatgag ccccaggtct gcgtgacctg gtacgaggcc gatgcgtatg
      781 ccaggtggcg cggcggtcgg ctccccaccg aggcggagtg ggagttcgct gcgcgcagcc
      841 ccgtgtccct ggtctatccg tggggcgacg agtacgacgc cgacctcgcg aacatcgacg
      901 gcgggacgaa gcccgtcgcg gtcggcagct acccggatgg cgcgagctgg gtcggcgcgc
      961 tcgacatggc cggcaacgcg atggagtggg tcgccgactg gtggagcaac acctactacg
     1021 cggagagccc ggcgatggac ccgccgggtc ccgcgtctgg cgtgatcaag atcgagaagg
     1081 gcggctggtg gggcccgccg gcaggcaccg gcccgttcgt gagccgcgcc tccttccggc
     1141 tcgacgagga ccccccgacc taccaggacc accacatcgg cttccggatc gtcaccgacg
     1201 actgatcgca gtcgtacgcc cggcgccatc ggggcttatt gcgactgagt ctcgataact
     1261 gctagcatcg gcccatggtc gatgccccga cgatcgtgag cgcgctcgag gacgccggat
     1321 accggctgac cacgccgcgc cgcgcgctcg ccgggtcgat cgcgggccgc cgcgggcact
     1381 tcaccgccga ggagctcctc accgaatccc gtggccggcg gcttggcgtg acacgcgcga
     1441 ccgtgttccg gacgctcgac gtcctcaccg acctcggcat cgtcgaacga ctcgacctgc
     1501 cgaatggcga gcatgcattc gtcgcctgcg aggccgagca ccaccatcac atcgtgtgct
     1561 cgtcgtgcgg ccgctccacg tgggtggccg atcccggtct cgagcgcgcg gcgacggcca
     1621 tcggtgagca gaccggctat cgaatcgaca cgcatcgact cgagctgttc gggctgtgcc
     1681 cggagtgcca ggcggccgac agatgaatcc agcggcgctg ctcccgttct tctcgaccct
     1741 cctcggcggc atggcggcgc tgcgcctccg acaccggatc cacgtcttca tggcgctcgc
     1801 cgcgggcgtg gtcctggcaa cggccgtggc cgatctcctg ccggaagcgt tcgagctggt
     1861 cggcgagggc ggggcgctct cggtcgggat cgcgacggtc atcggcttcg tcggcttcag
     1921 cctcgtcgag gcgttcctcc accagtcgag cttcgagcac gtcggcgagg gcgatcacga
     1981 gcacgcccag gcgcacgacc attcgctcgc ggccgcagtc gcggcacgcc cgccgtcccg
     2041 catcctcggg atcctgccgc cgctgagcct gatcatccac agcacgctcg acggcctcgc
     2101 gatcggcctg ggcttccagg ccggcgccga catcgggttg ctcgtcctca tcgcggtcct
     2161 cgcccacgac ttcgccgacg gcatgaacgt ggtgaccctc gccctggacg cagcccgtgg
     2221 ggagcgcctc gccgtcgtcc tccttctgct cgacgccctg gcgccgcttg ccggcgccgc
     2281 gctcagcgtc gtcgtgaccg tgacccccgc caccctcggc ctgctgctgg caacctttgc
     2341 cggggtgttc atcgccatcg gggcggggca cctgctgccc gagtcgcagc atcacgatcc
     2401 gcgtc
//
LOCUS       DATBSW010000270         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_273092, whole genome shotgun sequence.
ACCESSION   DATBSW010000270 DATBSW010000000
VERSION     DATBSW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273092"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VIF63_07625"
     CDS             <1..655
                     /locus_tag="VIF63_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239290.1"
                     /translation="HARGLVDVRELQRGQPRGHRALPRLRQLALGQRPPHAERNTLRH
                     RSRLGFALGFVLLPLLLSAPSTLGADPSPGSTPSPPIDPSRPPTCAERFPDEGPAGVD
                     LRLGCIVGSVVGVYTTAQSAMATPLSSYAIMFGIVIGIGVVAVWFVGRLLARRAGERL
                     APVMPVEWWVCGTCKSVNGAQAARCYSCGSTRPVGPTMSTDEHPGISQSFGSKRKSG"
     gene            complement(709..>1697)
                     /locus_tag="VIF63_07630"
     CDS             complement(709..>1697)
                     /locus_tag="VIF63_07630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8239291.1"
                     /translation="LVAGIVRDQGIDAELRYNGYLELAWARSHADHFPRDVEALAAVG
                     TKAHVVPREELSTEIGTSEYHGGLAVDAGGVVHPAKWFAALTGLAERAGAELHEGVRA
                     TAIRRQSDGRFVVETKRGAIIARDVMVATNGYTDGVAPALRRRIIPIGSYIIATEPLP
                     EDLARELSPNGRAYFDTRNFLSYWHVTADRRMLFGGRVSFFPTTVDRTAKLLHRRMLL
                     IHPQLAGYRIEFSWGGKVALTMDRMPHIGRTGGVMYAMGYSGTGLVMSNWLGTRAAEW
                     MGGGEPPALAKLRFPIVPAPFEGRPWFLPVVGELFRARDRLAAREKMTDPPA"
BASE COUNT          243 a          625 c          587 g          242 t
ORIGIN      
        1 ccacgcccga ggactggtgg acgtgcgcga attgcagcgc gggcaacctc gagggcaccg
       61 cgcgctgcca cgcctgcggc agctggcgct cggccaacgc ccgccccacg ccgagcgcaa
      121 cacgctgagg catcgctccc ggctgggatt cgcgctcggg ttcgtcctcc tgccgctgct
      181 cctctcggcc ccgtcgaccc tcggcgccga cccctcaccc ggctcgacgc ccagcccgcc
      241 gatcgacccg agccggccgc ccacctgcgc cgagcggttc cccgacgaag gccccgccgg
      301 cgtcgacctt cgcctcggct gcatcgtcgg ctcggtggtc ggcgtgtata caaccgcgca
      361 gtcggcgatg gccaccccgc tctcgagcta cgcgatcatg ttcgggatcg tgatcgggat
      421 cggcgtcgtc gcggtctggt tcgtcggccg gctcctcgcc cgacgcgccg gcgaacggct
      481 ggcgccggtc atgcccgtcg aatggtgggt ctgcgggacg tgcaagtcgg tgaacggcgc
      541 gcaggccgcc aggtgctact cgtgcggcag cactcgcccg gtcggcccga cgatgtcgac
      601 ggacgagcac cccgggatca gccagtcgtt cgggagcaag cgcaagagcg gctagggtcc
      661 cccgcgtccc cgctcgcaag gtagccaggg cgccgtgccg gctcgagctc aggccggtgg
      721 gtcggtcatc ttctcgcgcg cagccagtcg atcgcgcgcc cggaacaact cgccgacgac
      781 gggcaggaac cacggccggc cctcgaacgg tgcgggcacg atcgggaagc gcagcttcgc
      841 gagcgccggc ggctcgccgc cacccatcca ctcggccgcg cgcgtaccga gccagttcga
      901 catgacgagg ccggtccccg aatagcccat cgcgtacatc acgccgccgg tgcgcccgat
      961 gtggggcatc cggtccatcg tgagcgcgac cttgccgccc cacgagaact cgatgcgata
     1021 gccggcgagc tggggatgga tcaggagcat ccgccgatga agcagcttcg ccgtccgatc
     1081 gaccgtcgtc gggaagaagc tgacgcgccc gccgaacagc atccggcggt ccgcggtcac
     1141 gtgccagtac gacaggaagt tgcgggtgtc gaagtaggcc cggccgttcg gtgacagctc
     1201 ccgggcgagg tcttcgggca gcggctcggt ggcgatgatg tagctgccga tcgggatgat
     1261 ccgccggcgc agcgcgggcg ccacgccgtc cgtgtagccg ttcgttgcga ccatgacgtc
     1321 acgggcgatg atcgcgccgc gcttcgtctc cacgacgaat cggccgtccg actggcgccg
     1381 gatcgccgtc gcgcgaacgc cttcgtgcag ctcggcgccg gcgcgctcgg cgaggccagt
     1441 gagcgcggcg aaccacttcg cgggatggac aacgcccccg gcatcgaccg ccagcccgcc
     1501 gtggtattcg ctcgtcccga tctccgtcga gagctcctcg cgcgggacca cgtgggcctt
     1561 cgtgcccacc gccgcaaggg cctcgacatc ccttgggaag tgatctgcgt gcgagcgggc
     1621 ccaggcgagc tcgaggtagc cgttgtaacg aagctcggca tcgatgccct ggtcgcgcac
     1681 gatgcccgcg acaaggt
//
LOCUS       DATBSW010000271         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_872403, whole genome shotgun sequence.
ACCESSION   DATBSW010000271 DATBSW010000000
VERSION     DATBSW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872403"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VIF63_07635"
     CDS             complement(<1..828)
                     /locus_tag="VIF63_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8239292.1"
                     /translation="MTHAPTGDWNATSYDKVADPQTRWGAEVLERLPLQGDETVLDAG
                     CGTGRVTELLLAKLPRGRVIALDFSAAMLEQARGRLARFGDQVTYVQADLGQPLPLTE
                     KVDAILSTATFHWVPDHDAMFANLAAMLRPGGQLVAQCGGFGNIARFLDAARSVDPNF
                     ARTHNFQPADATTARLEGSGFDDIQTWLSEAPTYFEPGAPFEAFLETVCLRTYIDGLP
                     PEDRSPFIKAVAARLPEPMLDYVRLNIVARRAATPEEPGLPELHVVPLDSPAWGWRDG
                     "
     gene            complement(892..1407)
                     /locus_tag="VIF63_07640"
     CDS             complement(892..1407)
                     /locus_tag="VIF63_07640"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239293.1"
                     /translation="MILPKIRDPRFITVRRGGSLEDDDHHLLAIWAADCAEHVLPHFE
                     RDRPKDDRPRRAIELGRAWARGEVTWAEARTMAGHANAAARDLRGSARHAAYAAGQAA
                     AVGHVAAHELGAAAYAIRAARAAATEDDGLEAGRVECRWQRAQLTPEIRELVLDDQRL
                     RNELCWFVFDC"
     gene            complement(1431..2042)
                     /locus_tag="VIF63_07645"
     CDS             complement(1431..2042)
                     /locus_tag="VIF63_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8239294.1"
                     /translation="MKLTITTQISVDGVMQGPGGPEEDERGVFARGGWAHFDDEAGTA
                     MEEIYQRADAFLFGRRTYESFAGSWGTWPDPGDSPIWTALNSRPKYVAATTPYDPKWA
                     PATVLSGDLATAIRELKAKPGRELHVPGSGVLVRWLLDNDLVDEMTLFTFPVVVGRGT
                     RLFSDAGRYMALDLVGSRATPSGVTIQVYRPAGRPGSGDIAEP"
     gene            complement(2116..>3067)
                     /gene="selD"
                     /locus_tag="VIF63_07650"
     CDS             complement(2116..>3067)
                     /gene="selD"
                     /locus_tag="VIF63_07650"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8239295.1"
                     /translation="DHAAADGAAGLIAGLNPPDDAAVHLIAPGVAVIGTLDFFPPIVD
                     DPRTFGEIAAANALSDVFAMGGRVLFALSVAAIPETMPMPVFRAILEGAAAKVREAGG
                     MLAGGHTIKDPEPKYGLAVIGVADPDRLLRKGGAQPDDLLILTKPLGTGLLVSGHRQG
                     KTAEADLGAAVDHMRRLNRLASEILVRHGIRGATDVTGFGLLGHGLEMARASRTTFAF
                     DASKLPALSGALELARAGVETGGAAHNRRFVVPSLEVGPDVLPELITLAHDPQTSGGL
                     LAAIPRDTVKALHKDLVDAGIDHWPVGRVEASDQPGVRLT"
BASE COUNT          447 a         1055 c         1101 g          464 t
ORIGIN      
        1 gccatcgcgc cagccccagg cgggcgaatc gagcgggacg acgtgcagct cgggcagccc
       61 gggctcctcg ggcgtcgcgg cgcggcgggc gacgatgttc agccgaacgt agtcgagcat
      121 cggctccggc aggcgcgccg cgacggcctt gatgaacggc gatcgatcct ccggtggcag
      181 cccgtcgatg tacgtgcgca ggcagacggt ctcgaggaag gcctcgaacg gcgcgccggg
      241 ttcgaagtag gtcggcgctt ccgacagcca cgtctggatg tcgtcgaagc cgctcccttc
      301 gaggcgcgcc gtcgttgcgt ccgcgggctg gaagttgtgc gtgcgcgcga agttcgggtc
      361 gaccgaacgg gcggcgtcga ggaaccgcgc gatgttgccg aagccgccac attgcgcgac
      421 gagctggccg ccgggccgga gcatggcagc gagattcgcg aacatcgcgt cgtggtccgg
      481 cacccagtgg aaggtggccg tcgagaggat cgcgtcgacc ttctcggtga gcgggagcgg
      541 ctgcccgagg tccgcctgga cgtacgtcac ctgatcgccg aacctcgcaa gacgcccgcg
      601 tgcttgctcg agcatcgcgg ccgagaagtc gagggcgatc acgcgaccgc gagggagctt
      661 cgccagcagc agttccgtga cccgacccgt gccgcagccg gcatcgagca ccgtctcgtc
      721 gccctgcagc ggcagccgct cgaggacctc cgcgccccag cgcgtctgcg gatccgcgac
      781 cttgtcgtac gaggtcgcgt tccagtcgcc ggtcggtgcg tgcgtcatgc cgggactatg
      841 gcatctctcg gctctggggc gattttcgcg tgatcacgtg cgccagccgt gtcagcagtc
      901 gaacacgaac cagcacagct cgttgcggag ccgctggtcg tccaggacga gctcccggat
      961 ctcgggcgta agttgggcgc gctgccatcg gcactcgacg cggcctgcct ccaggccgtc
     1021 gtcctcggtc gcagccgcgc gcgccgccct gatcgcgtac gccgcggcgc cgagctcgtg
     1081 cgccgccaca tgccccaccg ccgcggcctg gcctgcggcg tatgcggcat gccgcgctga
     1141 accgcgcaag tccctcgccg cggcgttggc gtggccggcc atcgttcgag cctcggccca
     1201 cgtgacctca ccccgcgccc aggcgcgccc aagctcgatc gcgcgtcgtg gacggtcgtc
     1261 cttcggccgg tccctctcga agtggggcag cacgtgctcg gcgcaatcag ccgcccagat
     1321 cgcgagaagg tggtgatcgt cgtcctcgag gctgccgcca cgacgcacgg tgatgaaccg
     1381 cggatcacgg atcttgggga ggatcattcg aggtcgtccg ccagcctcgc tcagggttcc
     1441 gcaatgtcac ccgagccagg gcgcccggcc ggccggtaga cctggatcgt caccccgctg
     1501 ggagtcgccc gcgatccgac caagtcgagg gccatgtacc ggccggcgtc ggagaacagc
     1561 cgcgtgcccc tgccaacgac cacggggaag gtgaacaggg tcatctcatc gacgaggtcg
     1621 ttgtcgagca gccatcggac gagaacgccg ctgccaggca catgcaactc gcgtcctggc
     1681 ttggccttca gctcacgaat ggccgtcgcg aggtcaccgg agaggacggt cgccggcgcc
     1741 cacttcgggt cgtaaggtgt cgttgctgcg acgtacttcg gccggctgtt caaggcagtc
     1801 cagatcgggc tgtcgcccgg atccggccac gtgccccagg agcccgcgaa gctctcgtag
     1861 gttcgccggc cgaacaggaa cgcgtcggcg cgctggtaga tctcttccat cgccgtcccg
     1921 gcttcgtcgt cgaagtgggc ccatccgccg cgcgcgaata cgccgcgctc gtcctcttcg
     1981 ggcccgccgg gcccctgcat caccccgtcg acggagatct gcgtggtgat cgtgagcttc
     2041 atatcgaacc ccatgagatc agagagacgt tagatccggc tccgtttgct tccagcaggc
     2101 tcagtcagtg cgacgtcacg tgaggcggac tccgggctgg tcggaggctt ccacgcgacc
     2161 gaccggccag tggtcgatgc ccgcgtcgac gaggtccttg tgcagcgcct tgaccgtgtc
     2221 gcgcgggatc gcagcgagca ggccgcccga ggtctgcggg tcgtgggcca gtgtgatgag
     2281 ctcgggcagg acgtccggac cgacctcgag ggagggcacg acgaagcggc ggttgtgggc
     2341 ggcgccgccg gtctcgacgc ctgcgcgagc gagctccagg gcgccggaga gcgccggcag
     2401 cttcgacgcg tcgaaggcga acgtcgtgcg cgaggcgcgg gccatctcga gaccgtggcc
     2461 caggagcccg aagccggtga cgtcggtcgc gccgcggatg ccgtgtcgaa cgaggatctc
     2521 ggacgcgagg cggttcagtc gccgcatgtg gtcgaccgca gcgccgagat cggcctcggc
     2581 cgtcttccct tgccggtgcc cgctcacgag caggcccgtc ccgagcggct tggtcaggat
     2641 gaggagatcg tccggctgcg cgccgccctt gcggagcaat cgatcaggat cggcgacgcc
     2701 gatcaccgcg agcccgtact tcggctcggg gtccttgatc gtgtggccgc ccgcgagcat
     2761 cccgcccgcc tcgcgcacct tcgcggcggc gccttcgagg atcgcccgga acaccggcat
     2821 gggcatggtc tcggggatgg cggctaccga cagcgcgaac agcacgcgac cgcccatggc
     2881 gaagacgtcg gacagggcgt tcgctgcggc gatctcgccg aacgtgcgtg ggtcgtcgac
     2941 gatcggcggg aagaagtcaa gcgtgccgat caccgcgacg ccgggcgcga tgaggtggac
     3001 ggcggcgtcg tcgggcggat tgagaccggc gatcaggccg gccgcaccgt cggcggctgc
     3061 gtggtcg
//
LOCUS       DATBSW010000272         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_414553, whole genome shotgun sequence.
ACCESSION   DATBSW010000272 DATBSW010000000
VERSION     DATBSW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414553"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VIF63_07655"
     CDS             <1..262
                     /locus_tag="VIF63_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239296.1"
                     /translation="FDVGLMAAMGVLGFVLERFQVPVGPVVLGIILGGPLEERFVQAL
                     TASRGSLAVFFARPTAAALGIVALAVWLLPAVTAWRSSRNHR"
     gene            528..>3177
                     /locus_tag="VIF63_07660"
     CDS             528..>3177
                     /locus_tag="VIF63_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY8239297.1"
                     /translation="MIEQMSPTAVGEQADAWLGIARMLETRQMPPPEDADDFPTDAER
                     TATAAWIKASLDTYEAAHGGEPGRVTVRRLTSAEYAYAIRDLTGVDVRVGVDASTDSV
                     GGEGFANFGDVQSVQDATVERYLEAARQVADHAVIGSGPLAFYTDPGETGLELSALNR
                     IQELYASRGFRVVSGEGGRPFGFDRYAKAFYIAWHYKYRKALGASTATLVGLAAQEGI
                     TGRFAEHISEAVNRVGTAYPSRLTIERWLAIPAPTADVPASVAKARADCDALVKELVG
                     WPSWLFARGDLAAGGAGDESPLVFDDTTLTPVPTHAYAYRLNRKFAEGGFKVVPGPWK
                     VQLSVSALHASPGGTPAVLWRNPRIVIRKAAPLPEPDEPAAVTRRRQPGPILSTTALR
                     SVLPADEVARLGFGTSPDGSAIGPDDFATTETTSFVVDVPVEADQLAELHADAELGRD
                     RQAVVRVLIADRSALASRERVFLGDPKSDGYRSFRAGIAEYLELMPPNSHGEPNPADK
                     DPVPAPFDNTYNSPEHDAFVITVKYQRTDDFFTRNIVEGGDRARLDNAWNDLFGSWPY
                     HDAYLGMLLDHYEVKGTSRKIADMTEAAVATLPEAVRPHVRSLRAHYDAVSQALRRAE
                     PGHVTNAIAFASRAWRRPLTATEETRLREFYRSLRSVHRLDHDAAVRALVARILMSPA
                     FLYRIEATPGAIETALNDWEMASRLSFFLWSSIPDDELRRAAAAGELRDPAKLGEQVQ
                     RMTADPKTRRLATEFFGQWLGFYHFDQYRGIDTGRFPEFTEEVKASMYDEAVSTFEYI
                     VRQGRPVKEILHAEYAFLNKTLATFYGIDASVTSTDRVERVDGARAFDRGGALRLGSV
                     LTSTSAPLRTSPVKRGDWVLRRILGTP"
BASE COUNT          484 a         1136 c         1089 g          468 t
ORIGIN      
        1 cttcgacgtc ggcctgatgg cggcgatggg ggtactgggc ttcgtgctcg agcggtttca
       61 ggtgccggtg gggccggtcg tgctggggat catcctgggc gggccgctcg aggagcgatt
      121 cgtccaggcg ctgaccgcgt ctcgcggctc gctggcggtc ttcttcgccc ggcccacggc
      181 cgccgccctc ggcatcgtgg cgctcgcggt ctggttgctg cccgccgtca cggcctggcg
      241 ctccagccgc aatcaccgct gaaaatggac acttgggtcg ggcgacacta gacttccttc
      301 agtcttcggg tcggagagtg tggatgaagc acctgggttt gggcgctttg ctgctgtggg
      361 cgatcgcctc cgacatctcg gcgcagccgc gaagcctcgc gaccgcggct acggttcccg
      421 gcgccttggc cgcgtccgcg gcttcgacga cgcaccagac cttgacgcga tactgcgccg
      481 aatgccacgg cgccgaccgg ccgaaggccg gcctgaacat cggacgcgtg atcgagcaga
      541 tgtccccgac ggcagtaggc gagcaggcgg acgcctggct cggcatcgcc aggatgctcg
      601 aaacgcgtca gatgccgccg ccggaggatg ccgacgactt cccgacggac gccgaacgga
      661 ccgcgaccgc cgcctggatc aaggcctcgc tcgacaccta cgaagccgca catgggggcg
      721 agccggggcg ggtgaccgtg cgccggctga cgagcgccga gtacgcctac gcgatccgcg
      781 acctgacggg tgtggacgtc cgggtcggcg tcgacgcctc gaccgactcg gtcggcggcg
      841 aaggcttcgc gaacttcggc gacgtgcagt cggtgcagga cgccacggtc gagcgctatc
      901 tggaagccgc ccggcaggtc gccgatcacg ccgtcatcgg gtccggcccg ctggccttct
      961 acacggatcc gggcgagacc ggcctcgagc tgtcggccct caaccgcatc caggagctct
     1021 atgcgtcgcg tggcttccgt gtggtgtcgg gtgaaggcgg tcggccgttc ggcttcgatc
     1081 gctacgcaaa ggcgttctac atcgcctggc actacaagta ccgcaaggca ctcggcgcct
     1141 cgaccgccac tctcgtcggg ctcgccgccc aggagggcat caccggacgc ttcgcggagc
     1201 acatctcgga agccgtgaac cgagtcggca cggcataccc gagccggctg acgatcgagc
     1261 gctggctggc gatcccggcg ccgacggccg acgtcccggc ctctgtcgcg aaggcccgcg
     1321 ccgactgcga cgctctcgtg aaggagctgg tcgggtggcc gagctggctc ttcgcgcgcg
     1381 gagacctcgc cgctggcggc gccggcgacg agagcccgct cgtcttcgac gacacgacgc
     1441 tcacgcccgt gcccacgcac gcctacgcct atcggctgaa ccgcaagttc gccgagggcg
     1501 gcttcaaggt cgtccccggc ccctggaagg tgcagctctc ggtcagcgcg ctccacgcga
     1561 gccccggcgg cactccggcg gtcctttggc gcaacccgcg catcgtcatt cgcaaggcag
     1621 ccccgctgcc ggagcccgac gagcctgccg cggtgacgcg gcgccgccag ccgggaccga
     1681 tcctgtcgac gacagcgctc cgctccgtgc tgccggccga cgaagtcgcg cgactcggat
     1741 tcggcacgag tcccgacggc tccgcgatcg gccccgacga cttcgcgacc acggagacga
     1801 cgtcgttcgt ggtcgacgtg cccgtggaag ccgaccagct cgccgagctt cacgccgacg
     1861 cggagctggg acgagaccgc caggcggtcg tgcgggtgct gatcgcggat cgctccgcgc
     1921 tcgcgtcgcg cgagagggtg ttcctcggcg acccgaagag cgacggctac cgcagcttcc
     1981 gcgccggcat cgccgagtac ctcgagctga tgccgcccaa ctcgcacggc gagcccaatc
     2041 ccgcggacaa ggaccccgtc ccggcgccct tcgacaacac ctacaacagc ccggagcacg
     2101 acgcgttcgt gatcacggtc aagtaccagc gcaccgacga cttcttcacc cggaacatcg
     2161 tcgagggcgg cgatcgcgct cggctcgaca acgcgtggaa cgacctgttc gggtcgtggc
     2221 cctaccacga tgcctacctg ggcatgctgc tcgaccacta cgaggtgaag gggacgagcc
     2281 gcaagatagc ggacatgacc gaagccgccg tggcgacgct gcccgaggcg gtcaggccgc
     2341 acgttcgttc gctgcgcgcg cactacgacg ccgtctcgca ggccctgagg cgtgccgagc
     2401 cgggccacgt cacgaacgcc atcgcgttcg cgagccgcgc ctggcggcgg ccgctcaccg
     2461 cgaccgaaga gacgcgcctg cgcgagttct acaggtcgct gcgctccgtc caccgcctcg
     2521 atcacgacgc ggccgtccga gcgctcgtcg cccgaatcct gatgtcgccc gcgttcctgt
     2581 accggatcga ggcgacgccg ggcgccatcg agacggcgct caacgactgg gagatggcga
     2641 gccggctgag cttcttcctg tggtcgtcga tcccggacga cgagctgcgg cgcgccgccg
     2701 cggccggaga gctgcgcgac ccggcgaagc tcggcgaaca ggtccagcgc atgacggccg
     2761 atccgaaaac ccgacgcctg gcgaccgagt tcttcggaca gtggctcggc ttctaccact
     2821 tcgaccagta tcgagggatc gacaccggac gcttccccga gttcaccgag gaggtgaagg
     2881 cgtcgatgta cgacgaggcc gtctccacct tcgagtacat cgtgaggcag ggacgaccgg
     2941 tgaaggagat cctgcacgcg gaatacgcgt tcctgaacaa gacgctcgcg accttctacg
     3001 ggatcgacgc cagcgtgacc tccacggatc gggtcgaacg cgtcgacggt gcgcgcgcct
     3061 tcgaccgtgg cggggcgttg cggctcgggt cggtgctgac ctcgacctcg gcgccgctgc
     3121 gcacgagccc cgtcaagcgc ggggactggg tcctccggcg catcctcgga acgccga
//
LOCUS       DATBSW010000273         1962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_484535, whole genome shotgun sequence.
ACCESSION   DATBSW010000273 DATBSW010000000
VERSION     DATBSW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484535"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..>1962
                     /locus_tag="VIF63_07665"
     CDS             175..>1962
                     /locus_tag="VIF63_07665"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEY8239298.1"
                     /translation="MADITANTQPSDSDYDAGSIGVLKGLEGVRKRPGMYIGDTDDGS
                     GLHHLVYEVVDNSVDEHLAGHATHIDVIIHYDNSVTVEDDGRGIPVDMHPTEHRPAAE
                     LVMTALHAGGKFGGKGYKVSGGLHGVGVSAVNALSDWLKLEIQRGGKVYYQEYGRGIP
                     TTEFKQTGNSPDRGTKVTFHPDPQIFKNVLEFSFDQLAQKLRELAYLNSGLRITLKDE
                     RPGQDRRAEFKFEGGIATYVVDLNANKTTVSEVIAYTAEHEGCTVDVAMQWNDGYSEL
                     LTCFTNTIKNRDGGTHASGFRQAFTRTINNYANENKLLKDAKGGLSGEDLREGLTAVL
                     SVKLADPKYSNQAKDKLVSSEVATAVSAVVTEKLGQYLEQHPKEARTIVAKALLASRA
                     REAARKAREMVQRKGALELTSLPGKLADCQERDPQKSEIFIVEGESAGGSAKQGRNRT
                     IQAILPLRGKILNVEKVRFDRMLTSQELTTLITALGTGVGDEKDIAKLRYHKIILMTD
                     ADVDGSHIRTLLLTFFFRHFNELFEGGHVYIAQPPLYKVKKGKTELYLKNEAALEDFL
                     LETVCRESTLQRGGGAQPLTGAELMVLVRR"
BASE COUNT          393 a          691 c          599 g          279 t
ORIGIN      
        1 cgcacgcgag ccacgtgctg ctgcatcgag atcgggccga ctaccgcatc tccgagggcc
       61 agatttctta gtaactgtaa ccagttatct tggatcgaaa caccggcgcg gctcttcccg
      121 acgaggaacc gccgtggtac caacgtaaaa cggcctggaa agctggtcgc tgtaatggca
      181 gatatcacag caaatacgca gccttccgat tctgactacg atgccggatc gatcggggtc
      241 ctgaaggggc tggaaggggt ccggaagcgc cccggcatgt acatcgggga caccgatgac
      301 ggcagcgggc tgcaccacct cgtctacgag gtcgtcgaca actcggtcga cgagcacctc
      361 gccggtcacg caacgcacat cgatgtcatc atccactacg acaactcggt gaccgtcgag
      421 gacgacgggc gcggcatccc ggtcgacatg cacccgacgg agcaccgccc ggcggccgag
      481 ctcgtgatga ccgcgctgca cgccggcggc aagttcggcg gcaagggcta caaggtgtcc
      541 ggcggcctcc acggcgtcgg cgtgtcggcg gtgaacgcgc tctccgactg gctcaagctc
      601 gagatccagc gcggcggcaa ggtctactac caggagtacg ggcgcggcat cccgacgacc
      661 gagttcaagc agaccggcaa cagccccgac cgcggcacca aggtcacgtt ccatcccgat
      721 ccgcagatct tcaagaacgt cctcgagttc tcgttcgatc agctcgcgca gaagctccgc
      781 gagctcgcgt acctcaacag cggcctccgg atcacgctca aggacgagcg gccgggccag
      841 gatcgccgcg ccgagttcaa gttcgagggc ggcatcgcca cgtacgtcgt cgacctcaac
      901 gcgaacaaga ccacggtctc ggaagtcatc gcctacaccg cggagcacga gggctgcacc
      961 gtcgacgtcg cgatgcagtg gaacgacggc tactccgagc tgctcacctg cttcacgaac
     1021 acgatcaaga accgcgacgg cggcacccac gcctccggct tccgccaggc gttcacccgc
     1081 acgatcaaca actacgcgaa cgagaacaag ctgctcaagg acgccaaggg cggcctctcc
     1141 ggcgaggatc ttcgcgaggg cctcaccgcc gtgctctcgg tcaagctcgc cgaccccaag
     1201 tactcgaacc aggccaagga caagctcgtc tcgtcggagg tcgccacggc cgtctctgcc
     1261 gtcgtgaccg agaagctcgg ccagtacctc gagcagcacc ccaaggaggc ccgcacgatc
     1321 gtcgccaagg cgctcctcgc ctcgcgcgcc cgcgaggccg ctcgcaaggc ccgcgagatg
     1381 gtgcagcgca agggcgcgct cgagctgacc tcgctgccgg gcaagctcgc cgactgccag
     1441 gagcgcgatc cccagaagag cgagatcttc atcgtcgagg gcgagagcgc cggcggctct
     1501 gcaaagcagg gtcgcaaccg caccatccag gcgatcctgc ccctccgcgg caagatcctc
     1561 aacgtcgaga aggtccgctt cgaccgcatg ctgacgtccc aggagctcac caccctcatc
     1621 acggcgctcg gcaccggcgt cggcgacgag aaggacatcg cgaagctccg ctatcacaag
     1681 atcatcctga tgacggacgc cgacgtcgac ggcagccaca tccggaccct cctgctcacg
     1741 ttcttcttcc gccacttcaa cgagctgttc gagggcggcc acgtctacat cgcgcagccg
     1801 ccgctctaca aggtcaagaa gggcaagacc gagctctacc tgaagaacga ggccgcgctc
     1861 gaggacttcc tgctcgagac ggtgtgccgg gaatccacgc tccagcgcgg cggcggcgcg
     1921 cagccgctga ccggcgccga gctgatggtg ctcgtccggc gg
//
LOCUS       DATBSW010000274         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_273469, whole genome shotgun sequence.
ACCESSION   DATBSW010000274 DATBSW010000000
VERSION     DATBSW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273469"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..986)
                     /locus_tag="VIF63_07670"
     CDS             complement(54..986)
                     /locus_tag="VIF63_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEY8239299.1"
                     /translation="MTDLTTLVRVSRLYYELGETQEQIAALVGVTRPQVSRLLKEARA
                     QGVVEIRIVDRAAVESPTADSLRERFGLRAVHLAPSLDGPPELTRRRVGRLAGQALRS
                     AIRDGMVVGVGDGAAMAAVAEELDGDGLSVDATIVPLCGGFWRSGAGIEPFRRVGEAL
                     GSTVHTLHAPGLLDDAGVRDALMAHAGVRSVVDLWNRVDVAIFGIGGPSWSEATVGPR
                     AMADIRERGAVGEVLIAPFAIDGRFVAKSLRARTIAFDARALPGVALTIGVADGPTKV
                     APILGALRGKLLNVLVTDVSTAEAVLNLAVEAAA"
     gene            complement(1159..1578)
                     /locus_tag="VIF63_07675"
     CDS             complement(1159..1578)
                     /locus_tag="VIF63_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY8239300.1"
                     /translation="MTQSPTQDHVELDPEVRRALGHGHTIDITTIGRRSGEPRRIEMV
                     FHSFGGHLYISGTPNPGRTRAWLHNMRANPQITFHLKQTTKADLPATAREITAEPERT
                     EVMTKVASVWRRDPTEMVAHSPLVEVTIPGFEAPAAD"
BASE COUNT          272 a          646 c          644 g          284 t
ORIGIN      
        1 gacgaccttc gtccgcgtcg aaccgaggtc gatgccgagg accgcgccgg tcgtcatgcg
       61 gcggcctcga cggcgagatt cagcacggcc tcggcggtcg agacgtcggt gacgagcacg
      121 ttcaggagct tcccgcgcag cgcgcccagg atcggcgcga ccttcgtcgg cccgtcggcg
      181 acgccgatcg tgagcgcaac gccgggcagc gcccgggcat cgaacgcgat ggtccgagcc
      241 cggaggctct tggcgacgaa ccgaccgtcg atcgcgaacg gggcgatcag cacctcgccg
      301 actgcgccgc gctcccggat gtcggccatc gcgcggggac cgacggttgc ctcactccag
      361 ctcgggccgc cgatgccgaa gatggcaacg tcgacccggt tccagaggtc gaccacgctg
      421 cggacgccgg catgcgccat cagcgcgtcc cggaccccgg cgtcatcgag cagccccggc
      481 gcgtgcaggg tgtggacggt cgacccgagc gcctcgccga cccggcggaa cggctcgatg
      541 ccagcaccgg atcgccagaa gccgccgcac agcggcacga tcgtggcatc gaccgaaagc
      601 ccgtcaccgt cgagctcctc ggcgaccgcc gccatcgcgg cgccgtcacc gaccccgaca
      661 accatgccgt cgcggatggc gctccggagc gcctgccccg cgagccggcc gacccgccgt
      721 cgagtcagct cgggcggtcc atcgaggctc ggcgcgagat gcacggcgcg cagcccgaac
      781 cgttcccgca gcgaatcagc tgtgggcgac tcgaccgcag cccgatcgac gatccggatc
      841 tcgaccactc cctgtgcgcg cgcctccttg agcaggcgcg atacctgcgg ccgggtgacg
      901 ccgacgagcg cggcgatctg ctcctgggtc tcgccgagct cgtagtagag ccggctcacg
      961 cggacgagcg tcgtgagatc ggtcacgcgg cggcccgccc cgcgcacgtt tgtgctgaac
     1021 agatgtgcat gcccatcgcc gaatgatcgg ccagcgcggc gagcccgtca atcgatgggt
     1081 acgcctcggg gatcgatggt cgttcgcggg ccggcgaaaa ctatgccaat cgctgcataa
     1141 ccggtttccc ggcgggcgtc agtcggcggc gggtgcctcg aagccgggga tcgtgacctc
     1201 gaccagcgga ctgtgggcaa ccatctcggt cgggtcgcgt cgccagacgc tggccacctt
     1261 ggtcatcacc tcggtgcgct cgggctcggc ggtgatctcg cgcgccgtgg ccggcagatc
     1321 cgccttcgtg gtctgcttca ggtggaatgt gatctgcgga ttggcgcgca tgttgtgcag
     1381 ccaggcgcgc gtcctgcccg ggttcggcgt gccgctgatg tacaggtgcc cgccgaagct
     1441 gtggaacacc atctcgatgc gccgcggctc gcccgaacgc cggccaatcg tggtgatatc
     1501 gatcgtgtgg ccgtgcccga gggcgcgccg gacctccggg tcaagctcga cgtggtcctg
     1561 ggttggcgat tgggtcatgg ctgggcggtc ctcgaggctc gtggtgcgca ttcggcggtc
     1621 ggagagcagc tcggggtcga tgagctgccc gtcgaatgtt cgttgacgac gcaacgatca
     1681 tcccacgagt ttcgatggtc cgcaagcagg accggggcga accgcgaccc ggcgagcctc
     1741 tatcgtgacg cgatgcccgg tgagttcctg gtcgcggcca gcgtcgggta cctgcaggtc
     1801 ctcatgctcc tgagcctgct cggaccgatc tcgatcttct acaaca
//
LOCUS       DATBSW010000275         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_61951, whole genome shotgun sequence.
ACCESSION   DATBSW010000275 DATBSW010000000
VERSION     DATBSW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61951"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            766..1269
                     /locus_tag="VIF63_07680"
     CDS             766..1269
                     /locus_tag="VIF63_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239301.1"
                     /translation="MLIATAAALLVASPALAAKPTRTVIQQTGTVTIPAGFGCAFDVV
                     AHLQPGARITFTEFSDGRLAYHEHANAILTNADTGATLYHRARFSGVDVYDDATGLVS
                     SVTQGRVNVFFYPGDDGPFGTVAEPGAIYRFVGRISYLYDADTEAISDFSYSGSVTDV
                     CAELSEP"
     gene            complement(1348..1854)
                     /locus_tag="VIF63_07685"
     CDS             complement(1348..1854)
                     /locus_tag="VIF63_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239302.1"
                     /translation="MSLERESQLIVPTERPGLVLKSIGIDDLDAYFALVDRNRRHLNQ
                     RGDYPFEASATRDELATWLSADTDDVRFGIWLDGELVGRVYLGPVSPPQWAIGYWLGA
                     ESTGQGIATIACRAVIEHARRLGASEIHAQITKGNDASIAVVRRLGFEHVEDVGDQTR
                     WRLELALG"
     gene            complement(1913..2998)
                     /locus_tag="VIF63_07690"
     CDS             complement(1913..2998)
                     /locus_tag="VIF63_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8239303.1"
                     /translation="MDPSIRAMFRPDPGLAYLDTATYGLPPEPTMRVMREALDTWQAG
                     TASWVEWDRQSERARVAFARLIGAAASRVALLPSVAAGVAMVAAALEPGDRVVVPAAE
                     FRSVLYPLLVARERGVDVVEVDDLERLAEAITPGTKLVALSLVQMQTGRVLPVGEIVE
                     RAEAVGARVLLDATHGVPFVKLDDLIDRLDYVLVHAYKHLLCPRGVAFLVLRDELIGT
                     LPPTSASWRSSDAPYTQFFGGPLLHADGAARYDASLAWHPWVGAAESLELLVAWQAGG
                     ALEEPIALARDLAGRLGIEWGGGTLVCPPIADPEAVRAVLAEQRIKAAFRGTAIRFST
                     HVYNVTADIERAATAIRPLVAQPVGSR"
     gene            complement(3008..3349)
                     /locus_tag="VIF63_07695"
     CDS             complement(3008..3349)
                     /locus_tag="VIF63_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239304.1"
                     /translation="MAEDRTLQIWHGHDHNGTRTMTATLYADGHLGIEGQDLGEAVKF
                     FGGDHGEYEFAWSASTDAVPRIVELLGGQPGDDALALLKLWSEEHKGMDPGEFLKQGG
                     AEIGFWSRLGD"
     gene            3495..3752
                     /locus_tag="VIF63_07700"
     CDS             3495..3752
                     /locus_tag="VIF63_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY8239305.1"
                     /translation="MASTVGERGQVTIEKAIREELGVYAGDVAVQRVEGRKVVIEFVP
                     APHNRSLSGILRDKVRRVVDDESWETISDYAEMTPDPDRKL"
     gene            3770..4144
                     /locus_tag="VIF63_07705"
     CDS             3770..4144
                     /locus_tag="VIF63_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8239306.1"
                     /translation="MVVRYLVGSPKDQAARAAQLIDSDLEIGVSLVVIAETAHVLRSF
                     YRIPRADLVDVLIDLMTKVNVSPIEISTPEVIDALVRARALESAPITDALIAASARAH
                     GALPVYAFDKKFGRLGAATANP"
BASE COUNT          696 a         1416 c         1400 g          686 t
ORIGIN      
        1 agcaagcgct ccaacactgg cgcgccttca cctcagcgcc gaagccctag gtgacgggca
       61 cggcctgcgc tcgctggctc ctggctctgg gcgtcccatc gacatcgatc gtgaatgcac
      121 catcgccgcc ggcagcccca aattcgagtt ggttcgattc atgccgaacc atcgccgcgg
      181 tcccgcgaat cgcggaatgc tcgcaggcag cgtcgaagga ctcggaaatc gtgcggggaa
      241 agcggtcgaa gcgccatcgg tgctgcacct cccgccgacg aggatccacc ggcgcgttgc
      301 ggcgctccgc gcgtctaccg tcctggtcgc gctcgaggac gtttcgtcgc gggactgaac
      361 gtgcgcggtg gcacaggggt gcatcgaacg agcccgagat tccgcctcga acacgatggt
      421 tcaccttcat tcgaaccatc tgaaattcgc cccggtaggc aacgagagca cggcgtcgag
      481 cgtgcgccag ccagtcgggg aatgcgccgt ccggcggatc gagacggacg ccactgcggt
      541 gctagctgaa cagccccaca taggcgttga tcgccggctg cccaccgtga tgtcccatgc
      601 gggcttgacg gctcggggat cccgtacgga ccgcgagcat cgggcctgaa tcgaccattg
      661 tcatcgaagg cgccgccggt gtaggttcga ctctacgacg ccgaggcgat ggatcccggc
      721 gctcggcgcc gccttacgag tgggaggtgt cacatgatcc gtcggatgct catcgcgacc
      781 gccgcggcgc tcctggtggc ttcacctgcg ctcgcggcaa agccgacgcg gacggtcatc
      841 cagcagaccg gcacggtcac gattcctgcg ggattcggct gcgcgttcga tgtcgtcgcg
      901 catctgcagc cgggcgcgcg catcaccttc accgagttca gcgatgggcg tcttgcctat
      961 cacgagcatg ccaacgcgat cctcacaaac gcggacacgg gcgcgacctt gtaccaccgg
     1021 gccaggttca gcggggtcga tgtctacgac gacgcgaccg gactggtgag ctcggtgacc
     1081 cagggccggg tcaacgtctt cttctaccca ggggacgatg gccccttcgg cacggtggcg
     1141 gagcctggcg cgatctatcg gttcgtgggt cggatttcgt acctctacga cgccgatacc
     1201 gaggccatct cggacttcag ctactccggc agcgtgaccg acgtctgcgc cgaactgagc
     1261 gagccgtagc agacagctgc ctatgccggc gacgcgcata gccgagcgca taggaattct
     1321 ccggggcgat tccgcgcata tgaaggacta gccgagcgcg agctcgagtc gccagcgggt
     1381 ctggtccccg acgtcctcga cgtgctcgaa gccgagccgg cgcacgacgg cgatgctcgc
     1441 gtcgttgccc ttggtgatct gggcgtggat ctcggacgcg ccgagcctgc gcgcgtgctc
     1501 gatcacggcc cgacacgcga tggtcgcgat gccctggccc gtcgattccg cgcccagcca
     1561 gtacccgatc gcccattggg gcggactcac cggaccgaga tacacgcgcc cgacgagctc
     1621 cccatcgagc cagatgccga accggacgtc gtcggtgtca gcagagagcc acgtcgcgag
     1681 ctcgtcgcgt gtcgccgatg cctcgaatgg atagtcgcca cgctggttca ggtgccggcg
     1741 atttcgatcc acgagcgcga aatacgcgtc cagatcgtcg atgccgatgg acttgaggac
     1801 aaggccaggg cgctcggtcg gcacgatgag ctgcgactcg cgttccagtg acacgaggcg
     1861 cattgtccgc gccgacgcta tcggccggtc gtcgtgccgc aatacgaaca gctcatcgcg
     1921 acccgacggg ttgcgcgacg agcggtcgga tcgccgtcgc ggcgcgctcg atatcggcgg
     1981 tgacgttata gacatgcgtc gagaagcgga tcgcagtgcc tcggaaggct gccttgatgc
     2041 gttgctcggc caggacggct cgaacggcct cggggtcggc gatcggtggg cagacgagcg
     2101 ttcccccacc ccactcgatg cccagtcgcc ccgccaggtc acgagccagc gcgatgggct
     2161 cctcgagcgc tcctcccgcc tgccacgcca cgaggagctc caacgattcg gctgcgccga
     2221 cccacggatg ccaggcgagc gatgcgtcat agcgcgccgc gccgtcggcg tgcagcagcg
     2281 ggccgccgaa gaactgggtg tagggcgcgt ccgacgagcg ccagctcgcg ctcgtcggcg
     2341 gcagcgtgcc gatgagctcg tcgcgcagca ccaggaaggc cacgccgcgc gggcacagga
     2401 ggtgcttgta ggcgtggacg aggacgtagt cgaggcgatc gatgaggtcg tccagcttca
     2461 cgaacggcac gccgtgcgtg gcgtcgagca ggacccgggc gccgacggcc tccgctcgct
     2521 cgacaatctc gcccaccggc agcacccgac cggtctgcat ctggacgagg ctgagcgcca
     2581 cgagcttggt gcccggcgtg attgcctcgg cgagccgctc gaggtcgtcc acctcgacca
     2641 cgtcgacccc gcgctcacgc gcgaccagca acgggtagag gaccgatcgg aactccgcgg
     2701 cggggacgac cacgcgatcg cccggctcga gcgccgcggc caccatcgcc acgccggccg
     2761 ccaccgacgg caggagagcg acgcgtgacg ccgccgcccc gatcagccgc gcgaacgcca
     2821 cacgggcgcg ctcggactgg cggtcccact cgacccagct cgccgtgccg gcctgccacg
     2881 tgtcgagcgc ctcgcgcatg actcgcatcg tcggctcggg cggcagcccg tacgtggcgg
     2941 tgtcgagata cgccagcccc gggtcgggcc ggaacatggc tcggatgctc ggatccacgg
     3001 ctggagtcta gtcgccgagg cggctccaga agccgatctc ggcgccgcct tgcttcagga
     3061 attcgcccgg gtccatgccc ttgtgctcct cggaccacag cttcaggagt gccagcgcat
     3121 cgtcgccggg ctggccgccg aggagctcga cgattcgggg cacggcgtcg gtcgaggccg
     3181 accatgcgaa ctcgtactcg ccgtggtcgc cgccgaagaa tttgacggcc tcgccgaggt
     3241 cctgaccctc gatgccaagg tgcccgtcgg cgtacagcgt cgcggtcatc gtgcgcgtgc
     3301 cgttgtggtc gtgcccgtgc caaatctgga gcgttcggtc ttcggccatc ggcagcaatc
     3361 atcgatcttc gcgggccttc ggagtagtct cagcgggccg aacccgcgcc tgctcaactg
     3421 gacggcgatt gacctactcg ccgcgatcat gcatactcaa tcagcaatag tatgtattgc
     3481 ttggagcggt aggcatggca agcacggtcg gtgagcgagg tcaagtcacg attgagaagg
     3541 cgatccgcga agagctgggg gtgtacgcgg gcgacgtcgc ggtccagcgg gtcgaagggc
     3601 gcaaggtcgt gatcgagttc gtgcctgcgc cgcacaaccg ctcgctgtcg gggattctcc
     3661 gcgacaaggt gcgccgcgtg gtcgacgacg aatcgtggga gaccatcagc gactatgcgg
     3721 agatgacgcc ggatcccgat cgcaagctgt gatcagcctc gacacgtcgg tggtcgtgcg
     3781 gtacctggtc ggcagtccga aagaccaggc agctcgggcg gcccaattga tcgatagcga
     3841 tttggagatc ggggtctccc tcgtcgtgat tgccgaaacg gcgcatgtcc ttcggtcgtt
     3901 ctaccggatc cctcgtgcgg atctcgtcga cgtgctcatc gaccttatga ccaaggtcaa
     3961 cgtttcgcct atcgagattt cgacgcccga ggtcatcgat gccctcgttc gtgctcgggc
     4021 actcgaatca gcgccaatca ccgatgcttt gattgccgcc agcgctcgag cccatggcgc
     4081 tctccccgtc tatgcgttcg acaagaagtt cggacgactc ggcgcggcga ctgccaaccc
     4141 gtaatcaggc gtcggcggcg cgcacgacct cgagcatctc ctcgccgtag cgggcgag
//
LOCUS       DATBSW010000276         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_837564, whole genome shotgun sequence.
ACCESSION   DATBSW010000276 DATBSW010000000
VERSION     DATBSW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837564"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VIF63_07710"
     CDS             complement(<1..1308)
                     /locus_tag="VIF63_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY8239307.1"
                     /translation="MSPSILPSPKGWPSILLARAGAAKPADLDAAIAAGAFEGLRRAI
                     RELGPQGTIEEVRASGLRGRGGAGHVTADKWEAAARTEARSRYVVANGYGADPAASTD
                     RTLVETDPYAVIEGVAIAAFAIGADTGYVSVRSTAKQAIDRLQAAIDAATSRNFIGDD
                     ALGSGRRIELEVRPVQGAYMLGEETVLLKALDGKRGQPEQRPPHPATRGFRNAPTVVQ
                     NVQTLASVPWILRHKANAFAAIGSGAAPGTILVDLRGPERSGVAEVPFGTPLKDIVAL
                     GGKPARGRKLKAVVVGGPTGGILPASELDTPYTFEDLRAVGAHVGSGSVIAADDRACI
                     VDLARLLTRFAADEACGKTIPCRIGVRRIAEIGERLTTGTSRSGDLDLLVDLSADVVG
                     SGLCDHERLATLPMMSSLRYFRDEIDAHLLRGECPAGVCRPIRL"
     gene            complement(1305..1982)
                     /locus_tag="VIF63_07715"
     CDS             complement(1305..1982)
                     /locus_tag="VIF63_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY8239308.1"
                     /translation="MSLLSGLIPGFGMVKGMALTMRRFFEPKATVMYPEMPADVATKF
                     RGRLQLLYDEYGSLKCETCFQCAQACPIECIDMGGMDTKGRFHVHWGPPETYGERREE
                     SALRRSGRTVPDAAYEHFMPFDMTTVDDILDAHNHDPKHMLAILEATQAAFGFLPVAA
                     LKRISERTGAWYATIYGTASYYGHLRFEAPSNKTQAKEVRARRATDASYLDALGASLG
                     GKSGGAS"
     gene            complement(1979..2962)
                     /locus_tag="VIF63_07720"
     CDS             complement(1979..2962)
                     /locus_tag="VIF63_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEY8239309.1"
                     /translation="MGAGRRLLDDGLSALVIPFAPGLFAQDFNIGLLYFFAIGGMTVV
                     GLLMGGWSSFNKYSLLGGIRSAAQIVSYEIPLTLSVVGLVILAGTMSLNTIVLQQSAT
                     GWFTDWYAFRQPLGFLIFAIAATAEANRTPFDLTEADSEIVAGFATEYSGMRFGFFFF
                     AEYIGVFVISALTVVLFLGGWNAPFPVPHISWIVNPGDLGLGLLILVAIVPVLGTLLL
                     AAPVYLFRSKTRWWVALIIGFVLFNVVAGGLFTLWAYINFDWVAGLVWFLGKTFSLVF
                     LFVWMRGTLPRVRIDQLMGFAWKWLLPASLLNLFVTALAVLVIDAMKAAPV"
     gene            complement(2926..3330)
                     /locus_tag="VIF63_07725"
     CDS             complement(2926..3330)
                     /locus_tag="VIF63_07725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY8239310.1"
                     /translation="MTRTWDRLTLPGKALLILVPLLAFLTAGLIITLALFGQQILDIL
                     IFNLPIVRFVFAALVLLLLAPLVAFVIIYMEMKIIALMNLRMGPDRVGPWGSLLSTVH
                     GLKVLMKEDFTPTRADAVVFTWAPVVVFSTMA"
     gene            complement(3353..>4070)
                     /locus_tag="VIF63_07730"
     CDS             complement(3353..>4070)
                     /locus_tag="VIF63_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239311.1"
                     /translation="LWSFIERDEIVEMLAALTGQRMLFNYFRIGGVNGDLNHEFMSRL
                     GDWMSRAVGQIDSGMGLLNENEIFVRRASGLGVVDQETALRYCMTGSNLRATGVPFDL
                     RRTHPYSIYPELEFDIPTRTEGDCLARYLLRMEEIKQSLRIIDQCLEQMPDGPIMAKL
                     PRLIRPRPGRAWSGIEGPRGVYGAYAISDGSDQPFRLKIHDPSFIHLQAVGALLPGHL
                     LADTMAIMASLDPVMGGVDK"
BASE COUNT          680 a         1338 c         1474 g          578 t
ORIGIN      
        1 gaggcggatc gggcggcaga cgccggccgg gcactcgccg cgcaggaggt gcgcgtcgat
       61 ctcgtcgcgg aaatagcgga ggctgctcat catcggcagt gttgcgagtc gctcgtggtc
      121 gcacaggccc gatccgacaa catcggccga caagtcgacc agcaggtcga ggtcgccgct
      181 gcgcgaggtg ccggtggtga ggcgctcgcc gatctccgcg atgcgccgga cgccgatccg
      241 gcacgggatg gtcttgccgc acgcttcgtc ggcggcgaag cgggtgagca gccgagccag
      301 gtcgacgatg caggcgcggt cgtcggcggc gatcaccgag cccgagccga catgtgcgcc
      361 gacggcgcgg aggtcctcga atgtgtacgg cgtgtcgagc tcgctcgcgg gcaggatccc
      421 gccggtcggg ccaccgacca cgaccgcctt gagcttccga ccgcgggcgg gcttgccgcc
      481 gagcgcgacg atgtccttca gcggcgtgcc gaacgggacc tcggccacgc cactgcgctc
      541 cggcccgcgg aggtcgacga ggatcgtgcc cggcgcggcg cccgagccga tcgccgcgaa
      601 ggcattcgcc ttgtgtcgga ggatccacgg cacggacgcg agggtctgca cgttctggac
      661 gaccgtcggc gcgttgcgga agccgcgcgt cgccgggtgc ggcgggcgct gctcgggctg
      721 gccgcgcttg ccgtcgaggg ccttgagcag gacggtctcc tcgccgagca tgtacgcgcc
      781 ctggaccggt cggacctcga gctcgatgcg ccggccgctg ccgagcgcgt cgtcgccgat
      841 gaagttgcgc gaggtcgcgg cgtcgatggc cgcctggagg cgatcgatcg cctgcttcgc
      901 cgtcgagcgc accgacacgt agcccgtgtc cgcgccgatg gcgaacgcgg cgatggccac
      961 gccctcgatg accgcgtagg gatcggtctc cacgagggtg cggtcggtgc tcgcggcggg
     1021 gtccgcgccg tagccgttcg cgacgacgta ccggctgcga gcctcggtcc gggccgcggc
     1081 ctcccacttg tcggcagtca cgtggcccgc gccaccgcga ccgcgcaggc cgctcgcgcg
     1141 cacttcctcg atcgtccctt gcggccccaa ttcacggatc gcgcgtcgca gtccctcgaa
     1201 cgcgcccgcc gcgatggccg cgtcgaggtc cgccggcttg gccgcgccgg cacgggcgag
     1261 caggatcgac ggccagccct tgggggaagg caggatcgac gggctcacga ggcgccaccg
     1321 gacttgccgc cgagcgatgc cccgagcgcg tccaggtacg acgcgtcggt ggcgcggcgc
     1381 gcacgaacct ccttggcctg cgtcttgttc gacggagcct cgaagcggag atggccgtag
     1441 tacgaggcgg tgccgtagat cgtggcgtac cacgcgccgg tgcgctccga gatgcgcttg
     1501 agggccgcga ccggcaggaa cccgaatgcc gcctgggtcg cctcgaggat cgcgagcatg
     1561 tgcttcgggt cgtggttgtg ggcgtcgagg atgtcgtcga cggtcgtcat gtcgaagggc
     1621 atgaagtgct cgtaggccgc gtcgggaacg gttcggccgg accgtcgcag ggcggattcc
     1681 tcgcggcgct cgccgtaggt ctcgggcgga ccccagtgca cgtggaagcg gcctttcgtg
     1741 tccatgccgc ccatgtcgat gcactcgatc gggcaagcct gggcgcactg gaagcacgtc
     1801 tcgcacttga ggctgccgta ctcgtcgtac aggagctgga gccggccgcg gaacttcgtc
     1861 gcgacgtccg cgggcatctc cgggtacatc acggtggcct tgggctcgaa gaaccggcgc
     1921 atcgtcaggg ccatgccctt gaccatgccg aagcccggga tgaggccgga caggaggctc
     1981 ataccggcgc cgccttcatc gcgtcgatga cgaggacggc gagggcggtc acgaacaggt
     2041 tcagcagcga tgccggcagc agccacttcc aggcgaagcc catcagctgg tcgatgcgaa
     2101 cccggggcag cgtgccgcgc atccacacga acaggaacac cagcgagaag gtcttgccga
     2161 ggaaccagac cagaccggca acccagtcga agttgatgta cgcccacagg gtgaacaggc
     2221 cgcccgcgac cacgttgaac aggacgaagc cgatgatcag cgcgacccac caccgggtct
     2281 tgctccggaa caggtagacg ggggcggcga gcaggagcgt gccgagcacg ggcacgatcg
     2341 cgacgaggat cagcagcccg aggccgagat cgccggggtt gacgatccag ctgatgtggg
     2401 gcaccgggaa cggcgcgttc cagccgccga ggaagagcac gacggtgagc gccgagatca
     2461 cgaacacgcc gatgtactcg gcgaagaaga agaagccgaa gcgcatgccc gagtactcgg
     2521 tcgcgaagcc ggcgacgatc tccgaatccg cctcggtcag gtcgaacggc gtccggttcg
     2581 cctcggccgt tgcagcgatg gcgaagatga ggaagccgag cggctggcgg aaggcgtacc
     2641 agtccgtgaa ccagccggtc gcgctctgct gcaggacgat cgtgttcagg ctcatcgtgc
     2701 cggcgaggat cacgagcccc acgaccgaga gcgtcagcgg gatctcgtag ctcacgatct
     2761 gcgccgcgga gcggatgcca ccgagcagcg agtacttgtt gaagctcgac cagccgccca
     2821 tcagcagccc gaccacggtc atgccgccga tcgcgaagaa atacagcagc ccgatgttga
     2881 agtcctgcgc gaacagcccc ggtgcgaacg ggatgaccag cgcgctcagg ccatcgtcga
     2941 gaagacgacg accggcgccc aggtgaacac caccgcgtcc gcgcgggtcg gcgtgaagtc
     3001 ctccttcatg agcaccttca ggccgtgcac ggtcgacagg agcgagcccc acggcccgac
     3061 ccggtccggg cccatccgga ggttcatgag ggcgatgatc ttcatctcca tgtagatgat
     3121 cacgaatgcg accagcgggg ccaggagcag cagcacgagc gccgcgaaca cgaagcggac
     3181 gatcggcagg ttgaagatca ggatgtcgag gatctgctgc ccgaacagcg cgagcgtaat
     3241 gatcaggccc gccgtcagga aggcgagcaa cggcacgagg atgagcagcg ccttgcccgg
     3301 caaggtgagc ctgtcccagg tgcgggtcat cacggccatc agcgggtctc ggtcacttgt
     3361 cgacgccgcc catcaccggg tcgaggctgg ccatgatcgc catcgtgtcg gcgagcaggt
     3421 ggccgggcag cagggcgccc acggcctgga ggtggatgaa gctcgggtcg tggatcttga
     3481 gccggaacgg ctggtccgag ccgtccgaga tcgcgtacgc gccgtagacg ccgcgcggcc
     3541 cctcgatgcc ggaccaggcg cgacccggtc gcgggcggat cagccggggc agcttggcca
     3601 tgatcgggcc gtcgggcatc tgctcgaggc actggtcgat gatccgcagg ctctgcttga
     3661 tctcctccat ccggagcagg tagcgagcga ggcagtcacc ctcggtgcgg gtcgggatgt
     3721 cgaactcgag ctccgggtag atcgagtacg ggtgggtccg gcgaaggtcg aacgggacgc
     3781 cggtcgcgcg caggttcgag ccggtcatgc agtagcgcag cgccgtctcc tggtcgacga
     3841 cgccgaggcc gctcgcccgg cgaacgaaga tctcgttctc gttgagcagg cccatccccg
     3901 aatcgatctg gccgacggcg cggctcatcc agtcgccgag gcggctcatg aactcgtggt
     3961 tgaggtcgcc gttcacgccg ccgatgcgga agtagttgaa gagcatccgc tggccggtga
     4021 gggcggcgag catctcgacg atctcatcgc gctcgatgaa cgaccacagg
//
LOCUS       DATBSW010000277         5793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_308516, whole genome shotgun sequence.
ACCESSION   DATBSW010000277 DATBSW010000000
VERSION     DATBSW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308516"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VIF63_07735"
     CDS             <1..229
                     /locus_tag="VIF63_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239312.1"
                     /translation="LGAEAAVLGVVAPRDRGPATEVAHRLAAAYPRLVIAFGGRAAPS
                     SGDIPRLPNDLSSAVGALIERLAQRSASPPA"
     gene            267..1034
                     /locus_tag="VIF63_07740"
     CDS             267..1034
                     /locus_tag="VIF63_07740"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY8239313.1"
                     /translation="MRLQDKVSIITGGASGMGRVAARMFAAEGARVVVADVTAPAAQS
                     VVDEVTAAGGTAIAVVADVSKEADAKRMVDETIAAFGRVDVLYNNAGIMPEADHSVID
                     TSVEDWDRVMAVNVRGVFLGCKYAIPKMVEQGSGSVINISSFVALIGCSNPQDAYTAS
                     KGAVLALTKSLAVQFAPKGVRTNAICPGPVETPLLMSWLVKDEEAKRIRLARNPTGRF
                     GKPEEIVHMAIYLASDESRWTNGAAMVVDGGITVNYF"
     gene            complement(1058..1495)
                     /locus_tag="VIF63_07745"
     CDS             complement(1058..1495)
                     /locus_tag="VIF63_07745"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8239314.1"
                     /translation="MPDGFELTYELDLARTPDAVFALLADPESFRAVDPALIDYGPRE
                     PLAAGAEGWFRHRRGGMTANTTWRVTVHDAPTRLEVAITGSGYQMTESAHLEATATGT
                     RATFIDRVWPTSLPGRVLVALSGGIMRRDLRDRAARLQALLEA"
     gene            complement(1488..2258)
                     /locus_tag="VIF63_07750"
     CDS             complement(1488..2258)
                     /locus_tag="VIF63_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239315.1"
                     /translation="MAAITGRPAGASAPSDPMPFLLRLYPSRWRARYGDEFLELLEAR
                     PPSLRDRVDIVVGAVDARVNPQVEGASDREVRAPGDRSIRRLAVATGALLAVWGSVGA
                     MYMRPWNSGDWPYDQTLTTIGWIVGMAGAISAMWALALIATTYTRALGSAGVGGALFA
                     GVGLILASLGAGVLALLLIAVGTALLTWRLRGPMLDTPTALVFLAATFALVGGWVAFA
                     AGGGQDVRLLLGMYGYGPAWLLFGLRLRAPSVPAIIGA"
     gene            complement(2258..2578)
                     /locus_tag="VIF63_07755"
     CDS             complement(2258..2578)
                     /locus_tag="VIF63_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY8239316.1"
                     /translation="MSEPLGDIGRFAEPSLFILASLSEGPKHGYAIMTDVEQISGGHL
                     GPGTLYAALARLERRGLIEALEPEDRRRPYRLTGLGAKTLKTQLQGLSDFAKTGLRRL
                     EGPG"
     gene            complement(2575..3294)
                     /locus_tag="VIF63_07760"
     CDS             complement(2575..3294)
                     /locus_tag="VIF63_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239317.1"
                     /translation="MSALVRLYPAAWRARYGAEFETLLAERPPTARDLVDIVIAAADA
                     RISPQVAGAPGTPTPLSSRISGASAILGGLLWCVVILVASLDRAAGDLSLPVYAALGL
                     MLFSLPGRYMRRYAKSITFGVGVATLAFGALYAEIVPWDLWMFVPVVLIFGVMGPGAF
                     ALAAARAGIGAATRWRLVALVTPWPLISVVVVSMGLMPELLKAPVLLASLLPIGIAWM
                     ATGARLASGRPSTSMTTRGLA"
     gene            complement(3291..3578)
                     /locus_tag="VIF63_07765"
     CDS             complement(3291..3578)
                     /locus_tag="VIF63_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239318.1"
                     /translation="MLASPSRSLGRHVEPALWVLIALDGDPLDLSAMFDAVRSLDGPI
                     GHGTLVGALSRLERLGLVENWLADSRQAMYRLTTLGRVAARSASILKGQPA"
     gene            complement(3581..4069)
                     /locus_tag="VIF63_07770"
     CDS             complement(3581..4069)
                     /locus_tag="VIF63_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239319.1"
                     /translation="MHTRFGPGRAAGRRLGTFLAALAVAAAFAPSALAGGATVTRGDV
                     HAFATGIGQPITGRAQMVRTATGTTMVTIHVVGLLPNTTYASHVHAAACAVGDADGHY
                     KFNPAGAATPPNEIWPGPFTTNGAGIGNANTIALGTAGPTAVSVVVHAPGGAKIACAD
                     LT"
     gene            complement(4100..4588)
                     /locus_tag="VIF63_07775"
     CDS             complement(4100..4588)
                     /locus_tag="VIF63_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239320.1"
                     /translation="MYRQSKSVWLLATLVAWAMVMLSVPAALALPLAIGHAQYHAVLA
                     LVVLLPALVIALRKGERPTIASTAPIAGLAIFAVTQLVESIGGLGYGPDNDGRVNGLV
                     AFHDLGVAITPIGLLAVLVGLTAGVGALIGRRFRRPALAAAVSAFVLVVGGIGVAKLI
                     GF"
     gene            4704..>5793
                     /locus_tag="VIF63_07780"
     CDS             4704..>5793
                     /locus_tag="VIF63_07780"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8239321.1"
                     /translation="MTLDQTSAGGATDITTLQMVIGGETVDAADGQTFEVVNPATGKV
                     IATAPLGGKVDVDNAVAAAQAAFEAPKGWSSWAAGKRGRALARLAAVVKDHTEELAQL
                     ESRNTGKPITSARGEITGVSLVFDYYAGAANKVFGQTIPVSKPGLDLVLREPIGVVGL
                     IVPWNFPILMASWKVAPALAAGNACILKPASYTPLTAIRLGELALEAGIPAGVLNVVT
                     GPGGTAGASIAAHPGIGKVAFTGETTTGQEIMRLASGNVKKISLELGGKSPNIVFADA
                     DLEKFARESPYSVFDNTGQDCCARSRILVERSVHDRVVELFIAATGNVKVGDPADDAT
                     EMGCLVSLRQRDRVQEYIDVGRAEGADLV"
BASE COUNT          959 a         1985 c         2067 g          782 t
ORIGIN      
        1 gctcggtgcc gaggcggcgg tgctcggggt cgtcgcgcct cgcgatcgcg gtcccgcgac
       61 cgaggtggcg caccgtctcg ccgcagcgta cccacggctc gtcatcgcct tcggtgggcg
      121 agccgcaccg agttccggag acatcccacg gctgcccaac gacctgtcca gcgccgttgg
      181 cgcgctcatc gagcgactgg cgcagcggtc ggcctcgcct ccggcatagc attcagcgct
      241 caggagttcg aggagggagc gccaggatgc ggctgcagga caaggtttcg atcatcaccg
      301 gcggcgccag cggcatgggt cgggtcgcgg cccggatgtt cgccgcggag ggcgcgaggg
      361 tcgtcgtcgc cgacgtgacg gcgccggcgg cgcagtcggt cgtcgacgag gtcacggccg
      421 cgggcggcac cgcgatcgcg gtcgtcgccg acgtctcgaa ggaagccgac gcgaagcgca
      481 tggtcgacga gacgatcgcc gcgttcggcc gggtcgacgt gctctacaac aacgcgggga
      541 tcatgcccga ggccgaccac tcggtcatcg acacgagcgt cgaggactgg gaccgggtca
      601 tggccgtgaa cgtgcggggc gtgttcctgg gctgcaagta cgcgatcccg aagatggtcg
      661 agcagggctc tgggtcggtc atcaacatct cgagcttcgt cgcgctcatc ggctgctcga
      721 acccgcagga cgcctacacg gcgtcgaagg gcgcggtcct cgcgctcacg aagtccctcg
      781 ccgtccagtt cgcgccgaag ggcgtccgca ccaacgcgat ctgcccgggg ccggtcgaga
      841 cgcccctgct catgagctgg ctcgtcaagg acgaggaggc gaagcgcatt cgcctggctc
      901 gcaacccgac cgggcggttc ggcaagcccg aggagatcgt ccacatggcg atctacctgg
      961 cgtcggacga atcacgctgg acgaacggcg cggcgatggt cgtcgacgga ggcatcacgg
     1021 tgaactactt ctaggccgcc gcctcgaact cgcggaacta cgcctcgagg agcgcctgca
     1081 acctcgccgc tcgatcgcgc agatcccggc gcatgatccc gccggatagc gcgacgagca
     1141 cgcggccggg cagcgacgtc ggccagacgc gatcgatgaa tgtcgcgcgc gttcccgtag
     1201 cggtcgcctc gaggtgggct gactcggtca tctggtagcc cgatcccgtg atagcgacct
     1261 ccaatcgcgt cggagcgtcg tgcacggtga cccgccatgt cgtattcgcg gtcatgccgc
     1321 cgcgcctgtg cctgaaccag ccctccgccc cggccgccag cggctcccgc gggccgtagt
     1381 cgatcagcgc cgggtcgacg gcccgaaagc tctcggggtc cgcgaggagc gcgaatacgg
     1441 cgtcaggggt tcgggcgaga tcgagctcgt aggtgagctc gaagccgtca ggcaccgatg
     1501 atggcgggca cgctcggggc tcgcaggcgg aggccgaaga gcagccacgc cggtccgtag
     1561 ccgtacatgc ccaacaggag ccgcacgtcc tggccgccgc cggcggcgaa cgcaacccag
     1621 ccaccgacga gcgcgaaggt ggcggcaagg aagaccaggg ccgtgggggt gtcgagcatc
     1681 gggccgcgca gccgccaggt gagcagtgcc gtgccgaccg cgatgagcag cagcgcgagc
     1741 acgcccgcgc cgagtgaagc caggatgagg ccgaccccgg cgaacagcgc gccgcccacg
     1801 ccggccgagc caagcgcccg ggtgtaggtg gtggcaatga gcgcgagcgc ccacattgcc
     1861 gaaattgcac ccgccatgcc gacgatccag ccgatcgtgg tgagcgtctg gtcatagggc
     1921 cagtcgccgc tgttccacgg ccgcatgtac atggcgccga cggatcccca gacggccagc
     1981 agcgcgccgg tcgcgaccgc aaggcgccgg atcgatcggt cgccgggcgc ccggacctcg
     2041 cgatcgctgg cgccttcaac ctgcgggttg acccttgcgt cgaccgcgcc gacgacgatg
     2101 tcgacccggt cgcgcaggct cggcggacgc gcctcgagca gctcgaggaa ctcgtccccg
     2161 tagcgtgccc gccatcggga cgggtagagc cgcaggagga agggcatcgg gtcgctcggc
     2221 gcggaggcgc cggccgggcg gcccgtgatc gcggccatca gccggggccc tcgagccggc
     2281 gaaggccggt cttcgcgaag tccgacaaac cctggagctg ggtcttgagt gttttcgcgc
     2341 cgaggccggt gagccggtat ggacgacggc ggtcctcggg ctcgagggcc tcgatgaggc
     2401 cccgtcgctc gagtcgggcc agggctgcgt acagcgtccc gggaccaagg tgcccgccgg
     2461 agatctgttc gacgtcggtc atgatcgcgt agccgtgctt cgggccctcg ctcaatgagg
     2521 cgaggatgaa cagcgacggc tcggcaaatc ggccgatgtc gcccagtggt tcgctcatgc
     2581 cagtcccctt gtggtcatcg aggttgatgg tcttccggaa gcaagtcggg cgccggtcgc
     2641 catccaggcg atgccgatcg gcaagaggct ggccagcaac accggcgcct tgagcagctc
     2701 gggcatcaac cccatgctca cgaccaccac gctgatcagg ggccagggtg tcacgagcgc
     2761 gacgagtcgc cagcgagtgg cggcgccgat gcccgcccgg gcggcggcga gggcgaacgc
     2821 gcccggcccc atcacgccga agatgagcac gaccggcacg aacatccaca ggtcccacgg
     2881 cacgatctcg gcgtacaggg cgccaaacgc gagcgtggcc acgcccacgc cgaacgtgat
     2941 cgacttcgcg taccggcgca tgtaccgccc aggcaggctg aacagcatca ggccgagtgc
     3001 ggcgtacacg ggcagggaga ggtccccggc ggcacgatcc aggctggcga cgaggatgac
     3061 cacgcaccac aggagaccgc cgaggatcgc cgaggcgccc gagatccgcg agcttaaggg
     3121 cgtcggcgtg ccgggcgcac cggccacctg cggcgagatc cgggcgtcgg cggcggcgat
     3181 gacgatgtcg accaggtcgc gcgcagtcgg cgggcgctcg gccaggagcg tctcgaactc
     3241 cgcgccgtag cgggcccgcc atgcggctgg atagagccgg accagagcgc tcatgcaggc
     3301 tgccctttca ggatcgatgc cgagcgtgcc gccacgcgac cgagtgtcgt cagacgatac
     3361 atcgcctgac ggctgtcggc cagccagttt tccaccaatc cgagccgctc gaggcgtgac
     3421 agcgcgccga ccagcgtgcc gtggccgatg gggccatcga gcgaccgcac ggcgtcgaac
     3481 atcgcggaca ggtcgagtgg gtccccatcg agcgcgatga gaacccacag cgcaggttcg
     3541 acgtggcggc cgagcgagcg gctcggcgat gccagcacgg tcaggtgagg tccgcgcagg
     3601 cgatcttcgc cccacccggc gcatggacca cgaccgacac cgccgtcggg ccggccgtgc
     3661 ccagggcgat cgtgttggca ttgccgatgc ccgcgccgtt ggtcgtgaac ggacccggcc
     3721 agatctcgtt cggcggggtc gcggcgcccg cgggattgaa cttgtagtgg ccgtccgcgt
     3781 cgccgacggc gcaggccgcc gcatggacgt gggacgcgta ggttgtgttc gggagcaggc
     3841 ctacgacgtg gatcgtcacc atggtcgtgc cggtcgcggt gcggaccatc tgggcgcggc
     3901 cggtgatcgg ctggccgatg ccggtcgcga acgcgtggac gtcgccacgg gtgacggtcg
     3961 cgccgcctgc gagcgccgat ggagcgaacg ccgcggcaac cgcgagggct gccaggaagg
     4021 tgcctagccg ccgcccggcg gctcgaccag gaccgaatcg tgtgtgcatc ccattcctcc
     4081 ttcgacgtgt cattgccact cagaacccga tcaacttcgc cacgccgatg cccccgacca
     4141 ccagtacgaa cgcggacacg gcggccgcca gagccgggcg gcggaatcgg cgcccaatga
     4201 gcgctcccac gcccgccgtc aggccgacca gcacggcaag aaggccgatc ggcgtgatcg
     4261 cgacgccgag gtcgtggaac gcgaccaggc cgttgacgcg cccgtcgttg tcggggccat
     4321 agccaagtcc gccgatcgat tcgacgagct gggtgacggc gaagatggcg agtccggcga
     4381 tcggcgcggt ggacgcgatc gtcggtcgct cgccctttcg cagtgcgatc accagcgccg
     4441 gaaggagcac aaccagggcg aggaccgcgt ggtactgcgc atggccgatc gccagcggca
     4501 aagccagcgc ggcgggaacg ctgagcatga ccatggccca tgccaccagg gtggcgagaa
     4561 gccagactga tttcgattgc cgatacatcg tgactcggta tatcgcagcg cgatatagct
     4621 tgtcaagagt tcgaccggcc ccggcgtatg atcgcgggca cttcacctgt gcccaatgca
     4681 gcctcggaga ggacccaccc agcatgaccc tcgaccagac gtcggccggt ggcgccacgg
     4741 atatcacgac cctgcagatg gtcatcggcg gcgagaccgt cgatgccgcg gacggccaga
     4801 cgttcgaggt cgtcaacccg gcgacgggca aggtcatcgc gaccgcccca cttggcggca
     4861 aggtcgatgt cgacaacgcg gtcgccgccg cccaggccgc gttcgaagcg cccaagggct
     4921 ggtccagctg ggctgccggc aagcgcgggc gggcgctcgc caggctggcc gcggtcgtca
     4981 aggaccacac cgaggagctg gcccagctcg agagccgaaa caccggcaag ccgatcacga
     5041 gcgcacgcgg cgagatcacc ggcgtgagcc tggtcttcga ctactacgcc ggggcggcga
     5101 acaaggtctt cggccagacg atccccgtct cgaagcccgg cctcgacctg gtcctgcgcg
     5161 agccgatcgg cgtcgtcggg ctgatcgtgc cctggaactt cccgatcctc atggccagct
     5221 ggaaggtcgc cccggcgctc gccgccggca acgcctgcat cctcaagccc gcttcgtaca
     5281 cgcccttgac cgcgatccgg ctgggcgagc tggcgctcga ggccgggatc ccggccggcg
     5341 tcctcaacgt cgtgaccggg ccgggcggga cggctggcgc atcgatcgcg gcccatccag
     5401 gcatcggcaa ggtggcgttc accggcgaga cgacgaccgg ccaggagatc atgcggctgg
     5461 cctcgggcaa cgtgaagaag atctcgctcg agctcggcgg caagagcccg aacatcgtct
     5521 tcgcggacgc cgatctcgag aagttcgccc gcgagtcgcc gtattcggtc ttcgacaaca
     5581 ccgggcagga ctgctgcgcc cgcagccgga tcctggtcga gcgatccgtc cacgaccgag
     5641 tcgtcgagct gttcatcgcg gcaacgggca acgtcaaggt gggcgatccg gcggacgacg
     5701 ccacggagat gggctgtctt gtcagcctca ggcagcgcga tcgcgtgcag gaatacatcg
     5761 acgtggggcg cgctgaaggc gccgatctcg tcc
//
LOCUS       DATBSW010000278         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1119582, whole genome shotgun sequence.
ACCESSION   DATBSW010000278 DATBSW010000000
VERSION     DATBSW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119582"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..823)
                     /locus_tag="VIF63_07785"
     CDS             complement(131..823)
                     /locus_tag="VIF63_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8239322.1"
                     /translation="MKTILVVDDEPKIAELARDYLEHAGFDVVTAADGPAALDVVHRR
                     RPDLVVLDLGLPGLDGLDVTRELRRVSNLPIVMLTARDDELDKLLGLELGADDYLTKP
                     FSPRELVARVKAVLRRADRPAEAPETIDAGDLVLDLPRMRAELAGTAIELTPTEFQLL
                     ATMAARPGRIFTRGQLLDAVHGVAFESYERAIDSHVKNLRRKLEPDPRRPRYILTVYG
                     VGYRFADDRAND"
     gene            complement(895..1362)
                     /locus_tag="VIF63_07790"
     CDS             complement(895..1362)
                     /locus_tag="VIF63_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239323.1"
                     /translation="MRILAAILLIVVLALGGGLIANAAYQAGLSTAIATAGATGTTVT
                     PVVVPPYAYGYGWHGFGGPGFGLFEFLAGLFFLFLIFGLIRAIVFGRGPGGRGGWDRG
                     SWGPGGSGHSKWEGHARETFEDWHRRAHDPSDPGTAMPTASSPPTTPTPTGIA"
BASE COUNT          236 a          514 c          608 g          215 t
ORIGIN      
        1 aacacgcagc cgaagcctcg ccgggggccc cgaccccagt cgtcgcggcg gcggggctcg
       61 tcccacccgc gacggtgccg gtgctcccgg ccgggcgggt cgacggggtc cggcgcaccc
      121 tcggcagcca tcagtcgttc gcgcgatcgt cggcgaagcg gtaaccgacc ccgtagacgg
      181 tgaggatgta gcgcggccgg cgcgggtctg gctcgagctt gcggcgcagg ttcttgacgt
      241 gcgaatcgat cgcccgctcg tagctctcga acgccacccc gtggacggca tcgaggagct
      301 gcccccgcgt gaagatccgg ccgggacggg ccgccatggt cgcgagcagc tggaactcgg
      361 tcggtgtcag ctcgatcgcc gtgccggcga gctcagcgcg catgcgcggc aggtcgagca
      421 ccaggtcgcc ggcatcgatc gtctcgggtg cttcggccgg gcggtcggcc cggcgcagca
      481 cggccttgac gcgcgcgacc agctcgcgcg ggctgaacgg cttggtcagg tagtcgtcgg
      541 cgcccagctc gaggccgagg agcttgtcga gctcgtcgtc gcgggccgtc agcatcacga
      601 tcgggaggtt ggagacgcgt cgcagctcgc gcgtgacgtc cagcccgtcg agaccgggca
      661 ggccaaggtc gaggacgacg agatcgggcc ggcgccggtg gacgacgtcg agcgcggccg
      721 ggccatcggc cgcggtgacc acgtcgaagc ccgcgtgctc gaggtagtcg cgggcaagct
      781 cggcgatctt gggctcgtcg tcgacgacca ggatggtctt catcgggcgc atcgtagagc
      841 cggatgcgcc cccgaacgat gtccgggggc gcgaggcgtg ggagagggag cccgtcaggc
      901 gatgccggtc ggcgtcggtg tcgtcggtgg ggacgatgcg gtgggcatgg ccgtgccggg
      961 atcggatgga tcgtgggccc ggcggtgcca gtcctcgaag gtctcgcggg cgtgaccctc
     1021 ccacttcgag tggccggagc cgccgggtcc ccagctgccg cgatcccagc cgccgcgacc
     1081 gcccgggcca cggccgaaca cgatcgctcg gatcagcccg aagatcagga acaggaagaa
     1141 caggccggcc aggaactcga acaggccgaa gcccggcccg ccgaagccgt gccagccgta
     1201 gccgtaggca tagggaggga cgacgaccgg tgtcacggtc gtgccagtgg cgcccgcggt
     1261 cgcgatcgcg gtgctgaggc cggcctggta cgcggcgttg gcgatgaggc cgccaccgag
     1321 cgcgaggacg acgatgagga ggatggcggc aaggatgcgc atggtgcgaa ggactccttt
     1381 ggtcattcac gtcggcccgg acgtcggacc gcttgccagc actctgcaag cgagatgtgg
     1441 agccgatgtg gaggcgccgt ggcggctcga tggcgaacgg cgcggtggcc gggcgtccgg
     1501 cgcgacttga cagtgtgacg tcgcactgtc acaatgagcc catgcccgat gatgaacgct
     1561 attccctgac cga
//
LOCUS       DATBSW010000279         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1013723, whole genome shotgun sequence.
ACCESSION   DATBSW010000279 DATBSW010000000
VERSION     DATBSW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013723"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VIF63_07795"
     CDS             <1..416
                     /locus_tag="VIF63_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239324.1"
                     /translation="LEAQSMVGVPAGLTGAQSLTPLRGIAGGGIPWMLASGKAELSAT
                     GHLEIKVRGLVLATGANAGSNPAATFRGLVSCVRADGTFANILTDPFPATTGPASAGG
                     GNSKIEATVALPSPCIAPIVFVTSAGGSWFAATGR"
     gene            complement(484..1728)
                     /locus_tag="VIF63_07800"
     CDS             complement(484..1728)
                     /locus_tag="VIF63_07800"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239325.1"
                     /translation="MRLPPSLDVLHERNFARYLGSLIVSHIGSGMANIALAFAVLGFG
                     TPTDLGIVLLSREVPMIAFLLLGGVFADRLPRRRILISTEMVKGAAQVATAALLFSGS
                     ATVWSVAALQVVFGVANAFSRPTTIGLIKEIVTDGHLQQANALAQLTRSTLSIASPAL
                     GALIVTFGSPAIAIAIDSVTFFVSAAFIASMRISGTVRATATSVIGDLRDGWREFISR
                     RWVVAIIASFGLFQLTYFPALLVLGPTVAKDHLGGAAGWGAVLATEALGAVVGGLFAL
                     RLKVGRPLVIGELVVVPSGFLLLGLAVPLPLIAIAAIGFVVGVGFALGETLYVTAFQR
                     NIPEHALSRISSYDWLGSVALNPIGYALIGPVAAAIGIPETLVIAGVLNVLVCLSVVL
                     VPSVRAIRSDGPVETTAASATG"
     gene            2091..2228
                     /locus_tag="VIF63_07805"
     CDS             2091..2228
                     /locus_tag="VIF63_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239326.1"
                     /translation="MRVPDTTRAARFRWKEAFMFPLLLWFLGVPGLIVILLLLLGVIH
                     I"
     gene            2519..>3087
                     /locus_tag="VIF63_07810"
     CDS             2519..>3087
                     /locus_tag="VIF63_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239327.1"
                     /translation="MDKANEAGAGVAAEGAATTADEEILEAPARGSVDELLDAGAHDE
                     LDAMRHSTAHVMAEAVLDLFPGTKLGIGPAVADGFYYDFDVPRALTPDDLEAIEGRMR
                     ESVAANHAFVRKEVSFDDGRSVVDGQGQGYKVEILDDLASKAKAAGEGSPSISFYEHG
                     PFSDLCRGPHVASTGKIGPFKLLSVAGAY"
BASE COUNT          513 a         1041 c         1085 g          448 t
ORIGIN      
        1 tcctcgaagc gcagtcgatg gtcggcgtcc cggccgggct caccggcgcg cagtcgctga
       61 cgccgctgcg cgggatcgcc ggcggtggca tcccatggat gcttgcctcc gggaaggccg
      121 agctctcggc gaccggtcat ctcgagatca aggtgcgggg gctggtgctc gcgacgggcg
      181 caaatgccgg atccaacccc gcggcgacgt tccggggcct cgtcagctgc gtccgcgctg
      241 acgggacgtt cgcgaacatc ctcaccgatc cgttcccggc aacgaccggc ccggcatcgg
      301 ccggcggcgg caattcgaag atcgaagcga ccgtcgccct gccctcgccg tgcatcgccc
      361 cgatcgtctt cgtgacgagc gcgggcggct cgtggttcgc tgccacaggc cgctgaaccg
      421 aagcactgtc gcaccgaggc ccgggtttat cgacccgggc ctttcggtgt ccgggtatcg
      481 cgatcagccg gtggccgagg ctgccgttgt ctcgactggt ccgtcggacc tgatcgcccg
      541 cacggacggg acgaggacga cgctgaggca cacgagcacg ttgagcaccc cggcgatcac
      601 cagcgtttcg ggaatgccga tcgcagcggc gaccgggccg atgagcgcgt agccgatcgg
      661 gttcagggcc acggagccga gccagtcgta cgagctgatc cgcgacagcg cgtgctccgg
      721 gatgttgcgt tggaacgccg tcacgtagag cgtctcgccg agcgcgaagc cgacgcccac
      781 gacgaacccg atcgcggcga tcgcgatgag cggcagcggc accgcgagcc cgagcagcag
      841 gaagcccgac gggacgacga ccagctcacc gatgacgagc ggtcgaccga ccttcaaccg
      901 cagcgcaaac agtccgccga ccaccgcccc gagggcctcg gtggccagga ccgcgcccca
      961 gcccgcagcg ccgccgaggt ggtccttggc gacggtcggg ccgagcacca gcagcgccgg
     1021 gaagtacgtc agctggaaca gcccgaacga ggcaatgatc gcgaccaccc agcggcgcga
     1081 gatgaactcg cgccacccat cgcgcaggtc gccgatgacc gacgtcgccg tcgctcggac
     1141 cgtcccgctg atgcgcattg aggcgatgaa cgcggccgac acgaagaacg tcacggagtc
     1201 gatggcgatg gcgatggccg ggctgccgaa ggtcacgatc agcgcgccga gcgcaggcga
     1261 agcgatcgac agcgtgctcc gcgtcagctg cgcaagcgca tttgcctgct gcaggtggcc
     1321 gtcggtcacg atctccttga tcagcccgat cgtggtcggg cgcgagaagg cattcgcgac
     1381 gccgaacacg acctggaggg cggcgacgct ccagacggtc gcgctgcccg aaaacagcag
     1441 cgccgccgtc gcgacctgtg cggcgccttt caccatctcg gtcgagatga ggattcggcg
     1501 gcgggggaga cggtcggcga acacgccgcc gagcagcagg aaggcgatca tcgggacttc
     1561 gcgcgagagc agcacgatgc cgaggtcggt cggcgtcccg aacccgagca ccgcgaacgc
     1621 cagcgcgatg ttcgccatcc cgctcccgat gtgggacacg atgagcgagc cgaggtagcg
     1681 cgcgaagttg cgctcgtgga gcacgtcgag tgagggcggc aggcgcatgg gcagcgagtc
     1741 taggcagccc ggctcgcgcc cgtcggccca tggtttgtgt caggtgttac tcacccgggc
     1801 gctcgtcagg gtgtcggcgc cgagggggtc cgtcccacgc tcagccacag atcgggaagc
     1861 tggcaaatgc tcgtgccgaa ccgcattcga caggagaccg gtgactagtg cccgagtgag
     1921 taacgaatga catttgtcga ggttgttgcg ggccgtcggg gcctcgtcgc ctcgacctgt
     1981 cggtcgtagc cgccgaccat ggcggcgcgc caccacgagc acggcctgcg gcacctgcaa
     2041 tgtgccgttg acggttccgt gatcccgctg gcatcgcagc acgccgcggc gtgcgcgttc
     2101 ctgacaccac ccgggccgca cgctttcgat ggaaggaggc attcatgttt ccactcttgc
     2161 tctggttcct cggggtgccc ggcctgatcg tcatcctgtt gctgctgctc ggggtgatcc
     2221 acatctagtg cgctatagtc ccgatcaacg catcgatcgg gacagtcgat cgaaggcagg
     2281 tctcacagag agcctggtcg cgctggaagc caggcagatc gaccttcgaa cccgcaccac
     2341 ccgtgagcgg ccggtgaacc ggcccgggtc cgcccgttag cgcggcgcaa agcgcacgat
     2401 cgagcgccct cgggcgatcg acgtgaaggc gggtggaacc gcgggatgtc gagtcatcga
     2461 cctctcgtcc tgtcgaagga cgaggggtcg tttcgattct ctggaggatc tcggcaagtt
     2521 ggacaaggcg aacgaggcgg gcgcgggcgt cgcagccgag ggcgcggcca cgactgccga
     2581 cgaggagatc ctcgaggcgc cggcgcgcgg ctcggtcgac gagctgctcg atgcgggcgc
     2641 ccacgacgag ctcgatgcca tgcgccactc gacggcccac gtcatggccg aggccgtcct
     2701 cgacctgttc ccgggcacga agctgggcat cggcccggcc gtcgcggacg ggttctacta
     2761 cgacttcgac gtgccccgcg cgctcacgcc cgacgacctc gaggcgatcg aaggccggat
     2821 gcgcgagagc gtcgccgcga accacgcgtt cgtccgcaag gaggtctcgt tcgacgacgg
     2881 ccgctccgtc gtggacggcc agggccaggg ctacaaggtc gagatcctcg atgacctcgc
     2941 gtcgaaagcg aaggcggccg gggaggggag tccctcgatc agcttctacg agcacgggcc
     3001 gttcagcgac ctgtgtcgcg ggccgcacgt cgcctcgacg ggcaagatcg ggccgttcaa
     3061 gctgctgtcc gtcgcgggtg cctactg
//
LOCUS       DATBSW010000280         9242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1154357, whole genome shotgun sequence.
ACCESSION   DATBSW010000280 DATBSW010000000
VERSION     DATBSW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154357"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VIF63_07815"
     CDS             complement(<1..358)
                     /locus_tag="VIF63_07815"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY8239328.1"
                     /translation="MAGQPIHIYLGHGASGNAATMAPFVKGLRARGLAADAVDLPVRK
                     AEDAVPAWRAAVPDGPGTVAGGQSYGGRVSTLAAAAGAGYAGLVLFCYPLHPPGRADR
                     AAERSAHFPSIDCPVLL"
     gene            complement(360..1205)
                     /locus_tag="VIF63_07820"
     CDS             complement(360..1205)
                     /locus_tag="VIF63_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8239329.1"
                     /translation="MITIARLTVGEAARRRVLWVLVILSLVTTSLTAGGVSALVGGAR
                     DDGIDELQIKFVVSQILIFIAFMFGFVLVMTAAFFGSPAIATDLESGIAQAMLARPLR
                     RSSYLLGRWLGLAVVIVGYAVVSGFIAIGAVGIVSGYLPPDPILPVAYLAGQSLILLS
                     LTVLLSTRLPPIAGGAIAVVAYGLAWMAGVLGKIGLAIGTTSLVSVSDAVRFLLPTDA
                     LWQGVVYGLEPSFVINAVQNEFVARSSPFFADAPPSAAILIWSAIWLVVVLGLAVDQL
                     RRREL"
     gene            complement(1202..2158)
                     /locus_tag="VIF63_07825"
     CDS             complement(1202..2158)
                     /locus_tag="VIF63_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239330.1"
                     /translation="MPRVSLDLPAIDVTDLKKQYGQIEALAGLTMTVGRGEVFGFLGP
                     NGAGKTTTVKLLLGLARPTSGSGKVLGAPMGDLATRRKIGYLPELFRYQPWLKAREVL
                     MLHGELAGIAATRRGPAADEALGIVGLADRANDLVGKFSKGMQQRLGIGAALLGEPAL
                     IILDEPTSALDPVGRVDVRGIVRAASDRGAAVFLNSHLLSEVEQVCDRVAILDHGRVV
                     ASGELDDVLGRAETLIRVTGLSTTDLAAIEPFGPPTLDGDMLAIRPMDAERVPELVAQ
                     LVSMGAKVHEVRSGRSSLEQRFLALVARDRPPAGTPSAQRLR"
     gene            2210..2347
                     /locus_tag="VIF63_07830"
     CDS             2210..2347
                     /locus_tag="VIF63_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239331.1"
                     /translation="MFEIEMPCCGTPARFDESANSVDCETCGVVLDLAAPTTDALPLA
                     A"
     gene            2509..3015
                     /locus_tag="VIF63_07835"
     CDS             2509..3015
                     /locus_tag="VIF63_07835"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8239332.1"
                     /translation="MFETEAEIAEVQALFDAHLAHANKHMLNIVTPDRRVTARQVARY
                     LVGTKHVAFATVTSRGEPRVSPLDMLFIHGRFTLSTGGGATKIAHLRANPACSAVHVD
                     GDRIAVVVNGTVEWIPREHPDHDEIHRTWADTYESDPYSWSDGVVIFRIKPVSMWAYA
                     FHPEEFPG"
     gene            3188..3952
                     /locus_tag="VIF63_07840"
     CDS             3188..3952
                     /locus_tag="VIF63_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY8239333.1"
                     /translation="MTINMRALLAEAVGTFVLLFVGLLGVSHWVLITGGQGNPEAALL
                     ILPFAFGLGLLAAIAIGGAASGGHYNPAVTLAALFDNRIDWKNAIGYVVAQVLGALAG
                     TLAVSLVVPANFLTAGVSAPGPLADDLIGRELHAFSTEAILTAIFVAVILTVTRKAPK
                     QAILVIPLTLVGIHFVGIFISGASVNPARSLAPAIVTGDYDSLWVYLTAPFVGAIVGW
                     GAYRLLYPPDDELLTEGDAEMDDDDLDETEDDDPAI"
     gene            complement(3949..5505)
                     /locus_tag="VIF63_07845"
     CDS             complement(3949..5505)
                     /locus_tag="VIF63_07845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8239334.1"
                     /translation="MAESASDTLTRSEDAERPFPPGEYPIVVVGSGPGALQVAYFLSR
                     LGIEHAVISGDVGPGGMFRRWPFFQRLLSWTKPYAPAERTSRAFERWDWNSLLALEPG
                     LRGIAAEFMDGTSDFPSRPEMEANLGAFAERAGVKVRYGCRWESTERVETADGDRFIL
                     STTDGVYRCQYAIFAVGVAEPYSPSAAGIELAVHYADTRDAETYAGKRLFILGKQNSG
                     FELASGLLQWASRISLSSPSPAKTSIETRSLVGVRARYVQPFEDSALGGGVDILSASL
                     KGITRAQSGGLTVALERSDNAMPMSVEADEVIAATGFTCPLRDLPALGVATFGQSKLP
                     AQTEYWESATVPGIFFAGTITQGSAGLKKHGIPPNSGALHGYRYNARVLVREIGARHF
                     GIAPVKPSMHLGDVRDHLLAEASLAPELWHQKAFLASVVSFDPEAGVLDEGILPLTAF
                     LDGDGPDAVAMTVEADGSSIYPVVYVRRGGRQEEHALDPDPLHDYQTLAYKRALGAIL
                     DRLTAGASAA"
     gene            complement(5508..5936)
                     /locus_tag="VIF63_07850"
     CDS             complement(5508..5936)
                     /locus_tag="VIF63_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8239335.1"
                     /translation="MATRDEIADAIAGMAIFADLARPQLMGVASRFEEAFFPKGARVL
                     RQGLTGSGFYVILDGEADVIADGAKRATLARGEFFGEVSVLLGEPPIADIVATRPLRC
                     IVLGGPAVEGFLIDHPRVMFRMLQAQARRLRGANQRAAQN"
     gene            6085..6513
                     /locus_tag="VIF63_07855"
     CDS             6085..6513
                     /locus_tag="VIF63_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8239336.1"
                     /translation="MTLTQDRRAELLAGCPLFKGISPEGLAGLAEMATAVDFPAGHVI
                     ARQGEIGTGFFVVIDGMVRVVRDGEVVARLGPGEFFGELSVLDRMPRNATVAAETATK
                     CLALASWDFEKVLLEQPALTLSILRGVAARLRDVTESARH"
     gene            6522..>9242
                     /locus_tag="VIF63_07860"
     CDS             6522..>9242
                     /locus_tag="VIF63_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8239337.1"
                     /translation="MPSGALTFLFSDIEGSTRLLDALGSEPYTHILERQAALLRGAFT
                     RHDGREENTEGDSFFVVFDSALEAVLAAVEGQRALATEAWPAGVELNVRMGLHSGEAS
                     TSAAGLVGLDINRAARISAATHGGQIVVSDAVRSLVLADLGADISLRGLGNHRLKDLR
                     EPQPLSQVVAEGLRLEFPPLRSIDARPNNLPTQLTSFVGRERELAEVGALLAANRLVT
                     LTGPGGTGKTRLSLQAAANAAEDFPDGIFFVALETVRDPSLVPSRLAAAIGIAEPARR
                     TATDVLLEWLGGKQVLFVLDNFEQVLDAGPIVADLLRSATGLKVVATSRAPLRVSGEQ
                     EYPVPGLPTPPDITHLTSFELAQLPAAERKVDPATLSTYEAVRLFIARASAVKPGFEV
                     TNENAPAVAAICAQLHGMPLAIELAAARIKFLAPEAILTRLEQQLSVLAAGSRDLPER
                     QQTLRGAIAWSYDILNEACRLLLDRLSVFAGHIELEAAEAVCGPASELGQEVVDGLFS
                     LAEQSLIRAVESPTETGFAMLETIREFAFEMLEGRGQRDDLEKRHGAWFVDFAKRGAS
                     QLNGDDQRIWLERFEHAHENIRIAMDRAVAREDAETAITIGFNTWRFWQKRGHLYEAR
                     RRLETIAATDWSRRDPQLRARLMEALGGVAWWQGDIPTMAPAYREAVDLWRQIGDQRE
                     LANALYNYSFTFTIADSVPQAFLGDLDARAEGQRILDESLAIFRLIGDERGEANVLWG
                     KVNMLYFEGTFDPDSDDLRLALEKFRRIGDRTMEAWTLHMLGSALIRAGRLDDALAAL
                     KVALRLFYEASDTAGITLILDDLSSHAVSVGELERGAHFWGAARNLTTTTGAQLASMV
                     DESVELGNRPNVRTSLAPEVLDRLAREGGAMALDEVVAYALNISVDDLRAG"
BASE COUNT         1387 a         3272 c         3223 g         1360 t
ORIGIN      
        1 ggagcagcac cgggcagtcg atcgacggga agtgggcgga gcgctcggcg gcgcggtcgg
       61 ccctgccggg cggatggagc gggtagcaga acagcacgag cccggcgtag cccgcgcctg
      121 ccgcggcagc gagcgtcgag acgcggccgc cgtaggactg gccacccgcc acggttccag
      181 gcccgtcggg gacggcggct cgccaggccg gcaccgcatc ctccgccttg cgcaccggca
      241 ggtcgacggc atccgccgcg aggcctctcg cgcgcaggcc cttcacgaac ggcgccatgg
      301 tcgccgcgtt gccactcgcg ccatgcccga ggtagatgtg gatgggctgc cctgccacgc
      361 tatagctcgc gccggcgcag ctggtcgacg gcgaggccga gcacgaccac gagccagatc
      421 gcgctccaga tgaggatcgc ggcgctcggt ggcgcgtccg cgaagaacgg cgacgagcgg
      481 gcaacgaact cgttctggac cgcgttgatc acgaacgacg gctcgagccc gtagacgacg
      541 ccctgccaca gcgcgtcggt cggcagcagg aagcgcaccg cgtcgctgac cgagacgagg
      601 ctcgtggtgc cgatggccag tccgatcttg ccgaggactc cggccatcca ggccaagccg
      661 tacgcaacca ccgcgatggc gccgccggcg atcggtggca ggcgcgtcga caggagcacg
      721 gtcagcgaca gcaggatcag cgactggccg gcgaggtacg caaccggcag gatcgggtcc
      781 gggggcaggt agcccgacac gatcccgacc gcgccgatcg cgatgaagcc cgacaccacc
      841 gcgtagccga cgatcacgac cgcgagtccg agccaccggc cgagcaggta cgacgagcgg
      901 cgcagcggcc gggcgagcat cgcctgggcg atcccggatt cgagatccgt ggcgatcgcc
      961 ggcgagccga agaacgccgc ggtcatgacg agcacgaagc cgaacatgaa cgcgatgaag
     1021 atcaggatct gcgagacgac gaacttgatc tggagctcgt cgatgccatc gtcgcgggcg
     1081 ccaccgacga gcgcggacac gccccccgcg gtcaggctgg tggtgaccag ggagaggatg
     1141 acgaggaccc acaggacgcg ccgtcgggcg gcctcgccga cggtcagcct ggcaatcgtg
     1201 atcatcggag acgctgcgcg ctgggtgttc cggccggggg ccggtcgcgc gcgacgaggg
     1261 cgaggaagcg ttgctcgagg gagctcctgc cggacctgac ctcgtgcacc ttggcgccca
     1321 ttgacacgag ctgggcgacc agctcgggga cgcgctcggc gtccatcggg cggattgcga
     1381 gcatgtcgcc atcgagcgtc ggcggtccga acggctcgat cgccgcgagg tcggtcgtgc
     1441 tcaggccggt gacccggatc agcgtctcgg cccggcccag gacgtcgtcg agctcgccgc
     1501 tggcaacgac tcgaccgtgg tcgaggatcg cgacgcggtc gcagacctgc tcgacctcgc
     1561 tgagcaggtg cgagttcagg aagacggcgg cgccccggtc ggaggcggca cgcacgatgc
     1621 cccgcacatc gacccgcccg acggggtcga gcgccgacgt cggctcgtcg aggatgatga
     1681 gcgccggctc gccgagcagc gccgcgccga tgcccagacg ctgctgcatg cccttcgaga
     1741 acttgccgac caggtcgttc gcccggtcgg cgaggccgac gatccccagc gcttcgtcgg
     1801 cggcaggccc gcggcgggtc gcagcgatgc cggcgagctc accgtgcagc atgagcacct
     1861 cgcgcgcctt cagccacggc tggtagcgga acagctcggg caggtagccg atcttgcggc
     1921 gggtcgcgag gtcgcccatc ggcgcgccga ggaccttgcc cgagccactc gtgggccgcg
     1981 cgagtccgag caggagcttg acggtcgtgg tcttgcccgc gccgttcggc ccgaggaagc
     2041 cgaagacctc gccccgcccg acagtcatcg tgagcccggc gagggcttcg atctggccgt
     2101 actgcttctt caggtcggtg acgtcgatcg cgggcagatc gagcgaaacg cggggcatgc
     2161 ggcgcatcgt gacagacaag gtggcacgcc gttactgcac tttcttccca tgttcgagat
     2221 cgagatgccc tgttgcggaa ccccggcgcg gttcgacgag tcggccaatt cggtcgactg
     2281 cgagacctgc ggcgtggtcc tcgacctggc cgcgcccacc accgacgccc tgccgctcgc
     2341 cgcctgaccc cgaccgcacc tgccggcggc tggctgcgtt gccgagctcc gcgcgcccac
     2401 tcacgcacct tcgtgcgctc gcggtcgtgc tcgcttgtcg ccttggcctc gcctcggcat
     2461 ctgccgccgg gaatccgagc gttaggatgg ttcgcggagg ctgatgccgt gttcgagacc
     2521 gaggccgaga tcgcggaggt ccaggcgctg ttcgatgcgc acctggcgca cgcgaacaag
     2581 cacatgctca acatcgtgac gccggatcgt cgggtgacgg cccggcaggt cgcgcgctac
     2641 ctcgtcggca cgaagcacgt ggcgttcgcc accgtcacct cgcgcggcga gccacgcgtt
     2701 tcgccgctgg acatgctgtt catccacggc cgattcacgc tgagcacagg tggcggcgca
     2761 accaagatcg cgcacctgcg tgccaacccg gcctgcagcg cggtccacgt cgacggcgac
     2821 cggatcgccg tggtcgtgaa cggcacggtg gaatggatcc cgcgcgagca tcccgatcac
     2881 gacgagatcc accggacctg ggctgacacc tacgaatcgg atccgtactc gtggagcgac
     2941 ggcgtcgtga tcttccggat caagcccgtg tcgatgtggg cgtatgcgtt ccacccggag
     3001 gagttccccg gctgaccgcg cggcgcggtg gccgtcggcg catcgatcga ccaggcactc
     3061 gatcggctcg agctcgtcga ggtgctgtgc cgcacctggc gtgacgccct cctgatccgc
     3121 gcggccaaaa tcagccttcc gtcgcggact atccttccct ctcgcgagga agctccagga
     3181 gggaccgatg accatcaaca tgcgcgccct gctcgccgag gctgtcggca cgttcgtcct
     3241 gctgttcgtc ggcctgctcg gcgtttcgca ttgggtgctc atcaccggcg gacagggaaa
     3301 ccccgaggcg gcgctgctga tcctgccgtt cgcgttcggg ctcgggctgc tggcggcaat
     3361 cgcgatcggc ggcgcggcat cgggcggtca ctacaacccc gccgtcaccc tcgcggcgct
     3421 cttcgacaac cgcatcgact ggaagaacgc catcggctac gtggtcgcgc aggtcctcgg
     3481 cgcgctcgcc ggcacgttgg cggtgagcct ggtggtaccc gcgaatttcc tgaccgccgg
     3541 cgtctcagct cccggcccgc tggccgacga tctcatcggc agggagctcc acgcgttctc
     3601 gaccgaggcg atcctgaccg cgatcttcgt ggccgtgatc ctcaccgtca cccgcaaggc
     3661 gccgaagcag gcaatcctcg tgatcccgct gacgctggtc gggatccact tcgtgggcat
     3721 cttcatcagc ggcgcatcgg tgaacccggc ccgctcgctt gcccctgcga tcgtgaccgg
     3781 agactacgat tcgctgtggg tctacctcac tgcgccgttc gtcggcgcca tcgtcggctg
     3841 gggcgcgtat cggctcctct acccgcccga cgatgagctc ctcaccgagg gcgacgccga
     3901 gatggacgac gacgacctcg acgaaaccga ggacgacgac cccgccatct aggctgcgga
     3961 cgcgcccgcc gtgagcctgt cgaggatcgc gccgagcgcg cgcttgtagg ccagcgtctg
     4021 gtagtcgtgg agcggatcgg ggtcgagcgc gtgctcttcc tggcgtccgc cgcggcggac
     4081 gtagacgacc ggatagatcg agctgccgtc cgcctcgacc gtcattgcga ccgcgtccgg
     4141 gccgtcgcca tcgaggaagg ccgtcagcgg caggatgccc tcatcgagca cgcccgcctc
     4201 cgggtcgaag ctgacgacac tcgcgaggaa cgccttctgg tgccagagct cgggagcgag
     4261 gctggcctcg gcgaggaggt ggtcgcgcac gtcaccgagg tgcatcgacg gcttgaccgg
     4321 cgcgatcccg aagtgccgtg cgccgatctc gcggacgagc acgcgggcgt tgtaccgata
     4381 gccgtggagc gcgccggagt tgggcgggat gccgtgcttc ttgagcccgg ccgagccctg
     4441 cgtgatcgtg cccgcaaaga agatgccggg caccgtcgcg ctctcccagt actcggtttg
     4501 cgccggcagc ttcgattggc cgaacgtcgc gacgccgagc gcgggcaggt cgcgcagcgg
     4561 gcaggtgaac ccggtcgcgg cgatgacctc gtcggcctcg accgacatcg gcatggcgtt
     4621 atcgctgcgc tcgagcgcga ccgtgagccc acccgactgc gcacgggtga tgcccttcag
     4681 gctcgccgac aggatgtcca ccccgccgcc gagcgccgag tcctcgaacg gctggacgta
     4741 gcgcgcgcgc acgccgacga gcgagcgcgt ctcgatcgac gtcttggccg gtgacggcga
     4801 cgagagcgag atccggctcg cccactggag caggccggac gcgagctcga aaccggagtt
     4861 ctgcttgccc aggatgaaca gtcgtttccc ggcgtatgtc tcggcgtccc gggtgtcggc
     4921 gtagtgcacg gccagctcga tccccgcagc ggacgggctg tacggctctg ccacgccgac
     4981 cgcgaagatc gcgtactggc agcgatagac gccgtccgtc gtcgacagga tgaaccggtc
     5041 gccgtcggcg gtttcgacgc gctccgtcga ctcccagcgg cagccgtagc ggaccttgac
     5101 cccggctcgc tcggcgaagg cgccgagatt cgcctccatc tcgggtcgtg atgggaagtc
     5161 ggacgtgccg tccatgaact ccgcggcgat cccgcgcaac cccggctcga gggcgagcag
     5221 cgagttccag tcccagcgct cgaaggcgcg ggaggtccgc tcggcgggcg cgtacggctt
     5281 cgtccacgac agcagtcgct ggaagaacgg ccagcgtcgg aacatgccac cgggcccgac
     5341 gtcgccggag atgaccgcgt gctcgatccc gagccgcgac aggaagtacg cgacctggag
     5401 cgcgcccggc cccgagccga ccacgacgat cgggtactcg cccgggggga acggccgctc
     5461 ggcgtcctcc gagcgagtca atgtgtcgct cgcggattcg gccacggtca gttctgggcg
     5521 gcgcgctggt tcgcgccccg cagcctgcgg gcctgggcct ggagcatccg gaacatcacc
     5581 cgcgggtggt cgatcagaaa gccctcgacc gccgggccac cgagcacgat gcagcgcagc
     5641 ggccgggtcg cgacgatgtc ggcgatcggc ggctcgccga gcagcacgct gacctcgccg
     5701 aagaactcgc ctcgggcgag ggtggcgcgc ttggcgccgt cggcaatcac gtccgcctcg
     5761 ccgtcgagga tcacgtagaa gccggagccg gtcaggccct ggcgcaggac gcgggcgccc
     5821 ttcgggaaga atgcctcttc gaaccggctg gccacaccca tgagctgggg ccgcgccagg
     5881 tcggcgaaga tcgccatccc tgcgatcgcg tcggctatct cgtcacgggt cgccatcgag
     5941 ccctccaggt cacgcgggcg gctgcgcccg cggctcacca gggtgcgatt ctgcaccgcg
     6001 cggcggcggc gtgcctgcgc tcggttgacg ccgcgcgggc gcggggtcag catcgcagtt
     6061 gcccagatga cgttggagga ccggatgacc ctgacccagg accggcgcgc cgagctcctg
     6121 gccggctgcc cgctgttcaa ggggatctcc ccggagggcc tcgccggcct cgccgagatg
     6181 gcgaccgcgg tcgactttcc agccggtcac gtgatcgcgc gccagggcga aatcggcacg
     6241 ggcttcttcg tcgtcatcga cggcatggtt cgggtcgtcc gcgatggcga ggtcgtcgcc
     6301 cgactcggac ccggcgagtt ctttggcgag ctgtccgtgc tcgatcggat gccgcgcaac
     6361 gcgaccgtcg cggccgagac ggcgacgaag tgcctcgccc tcgcctcgtg ggatttcgag
     6421 aaggtcctgc tggagcagcc tgcgctcacc ttgtcgatcc tccgcggcgt cgcggcacgc
     6481 ctgcgcgacg tgaccgagtc cgcgcggcac tgaggccggc cgtgccgagc ggcgcgctca
     6541 cgttcctgtt ctccgacatc gagggctcga cccggctcct cgacgcgctc ggcagcgagc
     6601 cgtacacgca catcctggag cgccaggccg cgctcctgcg cggggcgttc acgcggcacg
     6661 atgggcgcga ggagaacacc gagggcgact cgttcttcgt cgtcttcgac agcgcgctcg
     6721 aggcggtgct cgccgccgtc gagggccagc gcgccctggc gaccgaggcg tggccggcgg
     6781 gcgtcgagct caacgtccgg atgggcctgc attccggcga ggcatcgacg tcggcggccg
     6841 gcctcgtcgg gctcgacatc aaccgggcgg cgcgcatctc cgcggcgacg cacggcggcc
     6901 agatcgtcgt ctcggacgcc gtccgctcgc tcgtcctcgc cgacctgggc gccgatatct
     6961 cgctccgcgg cctcggcaac caccggctca aggacctgcg cgagccgcag ccgctgtccc
     7021 aggtcgtcgc cgaggggctc cggctcgagt tcccgccgct gcgctcgatc gacgcccggc
     7081 cgaacaacct gccgacgcaa ctcacgtcgt tcgtgggcag ggagcgcgag ctcgccgagg
     7141 tcggcgcgct gctcgccgcg aaccggctcg tgacgctgac cgggccgggc ggcaccggca
     7201 agacacggct ctccctccag gccgccgcga acgcagccga agactttccc gacggcatct
     7261 tcttcgtggc gctcgagacg gtgcgtgatc cgagcctggt cccgtcccgg ctggccgccg
     7321 cgatcggcat tgccgagccc gcgcgacgca cggccaccga cgtgctcctc gaatggctcg
     7381 gcggcaaaca ggtcctgttc gtgcttgaca acttcgagca ggtcctcgac gccggcccga
     7441 tcgtcgccga tctgctgcgc agcgcgaccg gcctcaaggt ggtggccaca tcgcgggcgc
     7501 cgctccgcgt ctcgggcgag caggagtacc cggtgccggg cctgccgacg ccgccggaca
     7561 tcacccacct gacctcgttc gagctggccc agctgccggc cgccgagcgc aaggtcgatc
     7621 cggcgacgct ctcgacctac gaggccgtcc gcctgttcat cgcccgcgcc tcggcggtga
     7681 agcccggctt cgaggtgacg aacgagaacg ccccggcggt cgccgcgatc tgcgcgcagc
     7741 tgcacggcat gcccctcgcc atcgagctcg cggcggcgcg catcaagttc cttgcccccg
     7801 aggcgatcct cacgaggctg gagcagcagc tcagcgtcct cgcggcaggc tcgcgcgacc
     7861 tgcccgagcg ccagcagacg ctccgtggtg cgatcgcctg gagctacgac atcctcaacg
     7921 aggcatgccg cctgctcctc gaccggctct cggtgttcgc cggccacatc gagctcgagg
     7981 ctgccgaagc ggtgtgcggg ccggcgtcgg agctcggcca ggaggtcgtc gatgggctgt
     8041 tctcgctcgc cgagcagagc ctcatccgag cggtcgagag cccgaccgag accggcttcg
     8101 ccatgctcga aacgatccgc gagttcgcct tcgagatgct cgaagggcga ggccagcggg
     8161 acgacctgga gaagcggcac ggcgcctggt tcgtcgactt cgcgaagcgc ggcgcaagcc
     8221 agctcaatgg cgacgaccag cgcatctggc tcgagcggtt cgagcacgcc cacgagaaca
     8281 tccggatcgc gatggatcgc gccgttgccc gcgaggacgc ggaaacggcc atcacgatcg
     8341 gcttcaacac ctggcggttc tggcagaagc gcggccacct gtacgaagcc cggcgccgcc
     8401 tcgagacgat cgcggcgacg gactggtcac gccgcgaccc gcagctgcgc gctcggctga
     8461 tggaggccct cggcggcgtc gcgtggtggc agggcgacat cccgaccatg gcgccggcct
     8521 atcgcgaggc ggtggaccta tggcgccaga tcggcgacca gcgcgagctt gcgaatgccc
     8581 tctacaacta ctcgttcacc ttcacgattg cggactcggt cccgcaagcg ttcctcggtg
     8641 atctcgatgc ccgtgctgag ggccagcgaa tcctcgacga gtctcttgcg atcttccgcc
     8701 tgatcggcga cgagcgcggc gaagccaatg tgctgtgggg caaggtcaac atgctctact
     8761 tcgagggcac attcgatccc gactcggacg acctccggct cgccctcgag aagttccgca
     8821 ggatcggcga ccgaaccatg gaggcgtgga cgctgcacat gctcggcagt gcactgatcc
     8881 gtgccggcag gctcgacgat gcgctcgccg ctctcaaggt cgccctgcgg ttgttctatg
     8941 aggccagtga cacggccggg atcaccctca tcctcgacga cctgtcgtcg cacgccgtca
     9001 gcgtcggaga gctcgagcgc ggggcgcact tctggggggc agcgcgcaat ctgaccacga
     9061 ccactggcgc ccagctcgca tcgatggtcg acgagtccgt tgagctgggg aaccggccga
     9121 acgtccggac gtcgctggcg cccgaggtcc tcgaccgatt ggcgcgcgaa ggtggcgcga
     9181 tggcgctcga cgaggttgtt gcctacgccc tgaacatttc ggttgacgat ctgcgcgctg
     9241 gc
//
LOCUS       DATBSW010000281         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_273709, whole genome shotgun sequence.
ACCESSION   DATBSW010000281 DATBSW010000000
VERSION     DATBSW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273709"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VIF63_07865"
     CDS             <1..525
                     /locus_tag="VIF63_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239338.1"
                     /translation="GQTQASPFEGQPYALDLPDGWVAFDLSDPAGVAALDEFVAANPD
                     MGAAIEAFKALPNVTMAVNPLLGNVVVALPVPTGGQSLEVLAAAFTTQFGAVPGIVGV
                     PVAEDVSLRVGPAFRWHITIEANDPAGGTFQVGESIYLAGGETTAILVEFVEVGGSGV
                     AQEQQIIQSLRFTP"
     gene            558..1553
                     /locus_tag="VIF63_07870"
     CDS             558..1553
                     /locus_tag="VIF63_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY8239339.1"
                     /translation="MQKILNAPEPFVDEMLDGILAAHPAQLRRPSPRAIVRGDAPVPG
                     KVAIATGGGSGHLPVFMGYVGRGLIDGAAIGNVFASPSSDDMLDVTRAISAGAGVLYL
                     YGNYGGDRLNFDLAAELAADEGIEVRTVLGADDVASAPPERAASRRGIAGIFFLYKVA
                     GAAAAEGMSLAEVVAVTERAAAGTRTMGVALSPCTIPAAGVPTFDLPVGQMEIGMGIH
                     GEPGVRRGPLETADQIADELTRAILDDLPYRSGDEVAVLVNGLGATPKEELYILYRRV
                     ARLLADEGLRVHRVWVGEYATSLEMAGASLTLMRLDDELRRLVDAPAETPFFVQA"
     gene            1564..>1870
                     /locus_tag="VIF63_07875"
     CDS             1564..>1870
                     /locus_tag="VIF63_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEY8239340.1"
                     /translation="MTEPALEGPSAALAGLIRAIATDLLEHKDELNRLDGVAGDGDLG
                     LTVTTAARALIELAPELAGMAEADAIRKCGTEIARKAPSTGGTLIAFALMGAAKAAVP
                     "
BASE COUNT          262 a          672 c          665 g          271 t
ORIGIN      
        1 gggcagaccc aggcaagccc cttcgaaggc cagccctatg cgctcgacct gccggacggc
       61 tgggtcgcgt tcgacctctc ggatccggct ggggttgccg ccctcgacga gttcgtggcg
      121 gccaatcccg acatgggcgc cgcgatcgag gccttcaagg cgctgccgaa cgtgacgatg
      181 gcggtcaacc cgctgctcgg caatgtcgtc gtggcgctgc ccgtgcccac cggcggacag
      241 tcgctcgagg tcctcgctgc cgcgttcacg acgcagttcg gcgccgtgcc cggcatcgtt
      301 ggcgttcccg tcgccgaaga cgtcagcctg cgggtcgggc cggccttccg ctggcacatc
      361 acgatcgagg cgaacgaccc cgctgggggc acgttccagg tcggcgagag catctacctt
      421 gcggggggcg agaccacggc catcctcgtc gagttcgtcg aggtcggtgg cagcggcgtc
      481 gcgcaggaac agcagatcat ccagtcgcta cggttcacgc cctagcccgt ccactcgatc
      541 ctgtgggagg tcgcgacgtg cagaagatcc tcaatgcgcc cgagccgttc gtcgacgaga
      601 tgctcgacgg catcctggcc gcacacccgg cccagctccg acgaccctcg ccgcgcgcga
      661 tcgtgcgcgg tgacgcaccg gtgcccggca aggtcgcgat cgccacgggt ggcggctccg
      721 gccacctgcc ggtgttcatg ggctacgtcg ggcgcgggct gatcgacggc gccgccatcg
      781 gcaacgtgtt cgcctcgccg tcgagcgacg acatgctcga tgtcacgcgg gcgatctcgg
      841 cgggcgcggg cgtgctgtac ctgtacggca actacggcgg cgatcgcctc aacttcgacc
      901 tggcggccga gctggcggcc gacgagggca tcgaggttcg caccgttctc ggcgcggacg
      961 acgtggcctc ggcgccgccc gagcgcgcgg cctcgcggcg ggggatcgcc ggcatcttct
     1021 tcctgtacaa ggtcgcgggc gcggcggcgg ccgagggcat gtcgctcgcg gaggtcgttg
     1081 ccgtgacgga gcgcgccgcg gccggcacgc gcacgatggg cgtcgccctc tccccgtgca
     1141 cgatccccgc agccggcgtg ccgacgttcg acctgccggt cgggcagatg gagatcggca
     1201 tgggcatcca tggcgagccg ggcgtccggc gcggcccgct cgagaccgcc gaccagatcg
     1261 ccgacgagct cacccgggcg atcctcgacg acctgcccta tcgcagcggc gatgaggtcg
     1321 cggtgctggt caacggcctc ggcgcgacgc cgaaggagga gctgtacatc ctgtaccggc
     1381 gcgtcgcccg gctcctcgcc gacgaggggc tgcgcgtcca ccgggtctgg gtcggcgaat
     1441 acgcgacctc gctcgagatg gcgggtgcgt cgctgacgct catgcgcctc gacgacgagc
     1501 tgcgccgcct tgtcgacgct ccggccgaga cgccgttctt cgtccaggca tgaccgacca
     1561 accgtgaccg aaccagcgct cgaaggcccc agcgcagcgc ttgccgggct catccgcgcg
     1621 attgccacag acctcctgga gcacaaggac gagctcaatc gcctcgacgg cgtcgccggc
     1681 gacggcgacc tcgggctcac cgtgacaacg gccgcccgcg cgctgatcga gctcgcaccc
     1741 gagctggccg ggatggccga ggccgacgcg attcggaaat gtggcacgga gatcgcgcga
     1801 aaggcgccat cgaccggcgg gacgctgatc gcgttcgcgc tcatgggcgc cgcgaaggcg
     1861 gccgtgccgt
//
LOCUS       DATBSW010000282         8674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_873042, whole genome shotgun sequence.
ACCESSION   DATBSW010000282 DATBSW010000000
VERSION     DATBSW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873042"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="der"
                     /locus_tag="VIF63_07880"
     CDS             complement(<1..1073)
                     /gene="der"
                     /locus_tag="VIF63_07880"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8239341.1"
                     /translation="MAVGVPIVAIVGRPNVGKSTLFNRIVGQRAAIVEDRARTTRDRL
                     YGMADWNGRRFMLVDTGGLEIDPRDPIESRVQDQARIAIAEADVIVFVVEAITGLTPA
                     DEEAAAILRRTKAPVIVAVNKSDNDKRELEAAEFYALGWEHTFPVAAVHGRGVADMLD
                     EVVNALPPESDGELARKRREVEADEFSREVESGRLEPIIVGDADSEDATDDDADGADG
                     DAAPVEDEAGRKWDAMMAADSDDEPPAIALVGRPNVGKSSLLNALLGHERMIVSDIPG
                     TTRDAIDTRLPWGRTEIVLIDTAGIRRRGRVAGGADADRFSTLRSFRAISRADVAVLL
                     VDAVDGLTAQDMHIAGFVVDEGRG"
     gene            complement(1086..2060)
                     /gene="ispH"
                     /locus_tag="VIF63_07885"
     CDS             complement(1086..2060)
                     /gene="ispH"
                     /locus_tag="VIF63_07885"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY8239342.1"
                     /translation="MTTKGGTVSEIRIAKRTGFCYGVREAIDKAKEASAAGKVTHTLG
                     QVVHNEGVVRDLQSLGIETVDTLDDVDQGAAVVIRAHGVRPDVMERAAARGLEVIDGT
                     CTWVIQEQKEIVKLVEEGYTIVLLGTPKHPEVVGLLGFAPNAIVVDEEEDWDQIPRRK
                     KMALISQSTQPPWKFEKLAAFMVSRSHELKIVNTVCPVTIRRQEDTLEAAREVDLMVV
                     VGGRSSANTKELTRLCGIAGTRAIQIESVRDLTDAAAFEGAKVVGVTGGTSTPIEDLH
                     AVAQRILEMAGTPKAAKNAAALADAAFALAATPAGRTTSLPTFLSAAS"
     gene            complement(2111..2980)
                     /locus_tag="VIF63_07890"
     CDS             complement(2111..2980)
                     /locus_tag="VIF63_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239343.1"
                     /translation="MTTDRLASGRAFIVREGRLLERRLLATVFDGADAGGVIDVLRGY
                     RNADGGFGHGLEPDTRCPASLPIYVERALDAFIGAGVRDDPMIAAACEWLASVAAPDG
                     AVPLAFPIIEDYPRADHWTDWTYAPGLNPTAGIAARLIELGVSHPWLDRATEWCFTKL
                     ESLAAFDEDAHALGEVLEFLAVAPDRHRAAAIAPRVPGWLSNLAWFRSDPLDPGYGQT
                     PLHLAPAPNGPWSHLFNDAAIEGHLDRLERDQQDDGGWQITWQPPGQASTLEWRGVET
                     LRALRVLRAYGRL"
     gene            complement(2977..4488)
                     /gene="cmk"
                     /locus_tag="VIF63_07895"
     CDS             complement(2977..4488)
                     /gene="cmk"
                     /locus_tag="VIF63_07895"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY8239344.1"
                     /translation="MSTPSASRQLVVALDGPASSGKSSVGAAAAAELGYRFCDSGLLY
                     RAVTWLALHRRVPANDATGLVALVDEIELVDDGGGRLGHVSVDGVDRTAEVRGPDVDA
                     AVSAISGIGELRLAILARQRRLASAGSIVMAGRDTGTVVLPDADLKLFLDASVEERAH
                     RRVLERGIDPASPEAAEIVAELKRRDQLDSSRPIAPLRAADDAVVIRTDGNAFEQTVR
                     LVVDAIRTAETAHAAKGQAGDAADPTPKRDPRAPRAKRRPIRQTPVATRMNWIISLAS
                     LLFRTIARVVVRMKVEGDLDAVPRRGPVIVAANHASSADPVLIGAFLGQIIDRPLNWL
                     GKRELVEFPLTGWAMRLGGIHPVDRDAADLEAFRSAMRVLEAGQVLAVFPEGTRSRDG
                     GLQQVREGVGMLALRSGATVLPVAVVDSDLMWPRGQLLPHFGRHVTVRYGKPFVVGEE
                     LERTGAGAPKDRRAATEAATRLIMTRIAELLPPRQRGVYAADVDAAAVHGDPE"
     gene            complement(4559..7051)
                     /locus_tag="VIF63_07900"
     CDS             complement(4559..7051)
                     /locus_tag="VIF63_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY8239345.1"
                     /translation="MRAQRVDGRDRPQEGQILVLFAMVIVVIMLFASIVVDVGLLRNN
                     RQILVNAVDSAALAGGTKMPVDACNIAQSTASACTSINTVAWQSVRDLIIQTLQATYP
                     GIAPANYTISYRCLIGINSASPPAPYISRDVPLVCNPRGALGHTPVATDFKGAGATRY
                     SVCDPTLGDMCNVVVITGQATTSYTFGRVVGINEGYTGTTVSAACNGPCGQSPAAPVD
                     LVVLVDRTASMTDPQIVNARDATRSILSEYDPALQRVALGFLGPSEPNTSCNGTGGPA
                     VSVNAIDSSAPSAPAFSSSDSAATPLAGASSLTINRPGAASAAGHVLIAGITVNNSIL
                     TGGSGNVQIVAPAGWTSIRTTNNGSSVGLATFYKVGTASEPGTYQWTFRNASTGVLMT
                     VRATGGIARYTGVDNVNPINADGGNTGSDVSGTINATANAITATRANVALVAFLAMAN
                     GSGGTATDYFGTWTNSTTERFDRRGGNTAGPSIAAASKTVASVGATGATTATVAGGGS
                     YAAQHVALSPDVSNHYGTTYPADRAKWIPIGFTGTDSDTPAPAWNEAYVNGSGTLQNG
                     THIVSAINCFDHPGGTGTNLTTPIAMAAAYLQQYGRPNVKWGILFETDGQPSYGSTGD
                     PGNYTCASAVAAASAAKAITNANGENIELFTVGFFDSGSDPDCPDSSGTYSGQNVTRA
                     LAAMASTNQAPSPNGTANGCIAAENTDGDHFFCQPKTSDLAEVFSVIATQFAGIRTHL
                     IQLSPPPYVSSLTPSVGTRVGGTTVTVDGKYFTGTTSVTFGGASVPFVVVNDTQLVVN
                     SPSGANGSSVQVIVNNDGGSSLPHAGSSYTYN"
     gene            complement(7090..7572)
                     /locus_tag="VIF63_07905"
     CDS             complement(7090..7572)
                     /locus_tag="VIF63_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY8239346.1"
                     /translation="MRHGLGSPAGRRASNRRGQSLVEFALIFPILLVVLAGILDFGFL
                     LYSRMTVISAAREGARWAVYQSNVTAIPGDLNSQTGAIGANLTGLTWSDLSSTIACIP
                     NLANHNACDFAAGGVEDAEAGDSIRVAVTYTYRSFFSQLFGSTVPLGTQVQMVLEVPQ
                     "
     gene            complement(7572..7988)
                     /locus_tag="VIF63_07910"
     CDS             complement(7572..7988)
                     /locus_tag="VIF63_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY8239347.1"
                     /translation="MVEFALVIPVFLMILFAIFDFGFMAFSRLTLINATREGAHAATV
                     QSDNSVAIDENLDSPGGPIRANAPGIVQAGLTITTTCVPAAGLSACDFTGGNATRDAQ
                     TGDSVRVRTSYAYPSMFGRFLGTTVNLSTDVRMVLE"
     gene            complement(8169..>8674)
                     /locus_tag="VIF63_07915"
     CDS             complement(8169..>8674)
                     /locus_tag="VIF63_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239348.1"
                     /translation="GTGERPARVEVAGEDELARLADRYNQLTSELDRRSGALDAVRAE
                     IAAIDPTADSMGMVTAAAAGARRAFDMIEAEIVLGQPNVLPDTDHVPGEPIRVRATLD
                     LGSSGFGTLIGLLRPAHDWDPADQALLELFATGAALAIRNVQRDRVEKPNERSRAPRR
                     RKPGAGD"
BASE COUNT         1270 a         2947 c         3072 g         1385 t
ORIGIN      
        1 ccgcggccct cgtcgacgac gaagccggcg atgtgcatgt cctgcgccgt cagcccgtct
       61 acggcatcca cgagcagcac cgcgacgtcg gcgcgagaga tcgcgcggaa cgaccgcagt
      121 gtcgagaatc gatcggcgtc cgcgccaccg gcgacacggc cacgacgccg gatgcccgcc
      181 gtatcgatga gcacgatctc ggtccggccc cagggcagtc gcgtgtcgat ggcgtcgcgg
      241 gtcgtgcccg gaatgtcgga cacgatcatt cgctcgtggc cgagcagcgc gttgagcagg
      301 ctcgacttgc cgacgttggg ccgcccgacg agcgcgatgg ccggtggctc gtcgtccgag
      361 tcggccgcca tcatcgcgtc ccacttccgc ccggcttcgt cctcgaccgg tgcggcatcg
      421 ccgtcggcgc catcggcgtc gtcgtcggtc gcgtcctcgg aatcggcatc gccgacgatg
      481 atcggctcca gccggcccga ttcgacctcg cgcgagaact cgtcggcttc gacctcgcgg
      541 cgcttgcggg ccagctcgcc gtccgattcg ggcggcagcg cgttgacgac ctcgtcgagc
      601 atgtcggcga cgccccggcc gtggactgcc gcgaccggga aggtgtgctc ccagccgagg
      661 gcgtagaact cggccgcctc gagctcgcgc ttgtcgttgt cgctcttgtt gaccgcgacg
      721 atgaccggcg ccttggtgcg acgcaggatc gccgcggcct cctcgtccgc cggcgtcagc
      781 ccggtgatcg cctcgacgac gaacacgatg acatcggcct ccgcgatggc gatccgcgcc
      841 tggtcctgga cgcgcgactc gatcgggtcc cgtgggtcga tctcgaggcc gcccgtgtcg
      901 acgagcatga accggcgacc gttccagtcg gccatgccgt acaggcggtc acgcgtggtc
      961 cgcgctcgat cctcgacgat cgccgcacgc tggccgacga ttcggttgaa gagcgtggac
     1021 ttcccgacgt tgggacgacc gacgatggcg acgatgggaa ccccgacggc catggtgccg
     1081 gcggcctagc ttgcggccga gaggaacgtg ggcagcgagg tggtgcggcc ggccggggtc
     1141 gccgcaagag cgaacgctgc gtcggccagg gccgccgcgt tcttcgcggc cttgggcgtg
     1201 cccgccatct cgaggatgcg ctgcgcgacc gcgtgcaggt cctcgatcgg ggtcgacgtt
     1261 ccgccggtga cgccgacgac cttcgcgccc tcgaacgccg ccgcgtcggt gaggtcccgc
     1321 accgattcga tctggatcgc ccgcgtcccg gcaatcccgc acaggcgcgt gagctccttc
     1381 gtgttcgccg agctccgacc gcccacgacg accatgagat ctacctcgcg cgccgcctcg
     1441 agcgtgtcct cctgacggcg aatggtcacg gggcagaccg tgttgacgat cttcagctcg
     1501 tggctgcgcg aaaccatgaa cgcggcgagc ttctcgaact tccacggcgg ctgggtcgac
     1561 tggctgatca gcgccatctt cttgcgccgc gggatctggt cccagtcctc ttcctcgtcg
     1621 acgacgatcg cgttgggtgc gaagccgagc agcccgacga cctcgggatg cttcggcgtg
     1681 ccgaggagca cgatggtgta gccctcctcg acgagcttca cgatctcctt ctgctcctgg
     1741 atcacccagg tgcaggtgcc gtcgatgacc tcgaggccgc gcgccgccgc gcgctccatc
     1801 acgtcgggac gcacgccgtg ggcgcggatg acgaccgccg cgccctggtc gacgtcatcg
     1861 agcgtgtcga ccgtctcgat cccgagagac tggaggtcgc ggaccacgcc ctcgttgtgc
     1921 acgacctggc cgagggtgtg cgtcaccttc ccggccgcac tcgcctcttt cgccttgtcg
     1981 atcgcctcgc ggacgccgta gcagaacccg gttcgcttcg cgatccggat ctcggagacg
     2041 gtgccgccct tcgtggtcat gtcgtaccaa gtatcccacg ggcccgaact cggcccgctt
     2101 gagcgatcga ctagaggcga ccgtacgcgc gcaggacccg cagcgctcgc aacgtttcga
     2161 cgccgcgcca ctcgagggtc gaggcctggc ccggcggctg ccaggtgatc tgccagccgc
     2221 cgtcatcctg ctggtcgcgc tcgaggcggt cgaggtggcc ctcgatcgcg gcgtcattga
     2281 aaaggtggct ccacgggccg ttcggcgcgg gtgcgagatg cagtggcgtc tggccatagc
     2341 ccggatcgag tggatcggac cggaaccacg ccaggttcga aagccatccc ggcacgcgag
     2401 gcgcgatcgc tgccgcccgg tggcggtcgg gcgcgaccgc caggaactcg aggacctcgc
     2461 cgagggcgtg ggcgtcctcg tcgaaggcgg cgagcgattc gagcttggtg aagcaccact
     2521 cggtggcacg gtcgagccac gggtggctga cgccgagctc gatcagcctg gcggcgatcc
     2581 cggccgtcgg gttcaacccg ggtgcgtagg tccaatctgt ccagtggtcg gcccggggat
     2641 agtcctcgat gatcgggaac gcgagtggca ccgccccgtc gggagccgcc acgctcgcaa
     2701 gccactcgca cgcggcggcg atcatcgggt cgtcgcgcac gccggccccg atgaacgcat
     2761 ccagcgcgcg ttcgacgtag atcggcaggc tggcggggca acgggtgtcg ggctcgagcc
     2821 cgtgcccaaa gcctccgtcc gcgttccgat acccacggag cacgtcgatg acgccgcccg
     2881 catcggcccc gtcgaagacc gtggcaagca gacggcgctc gagcagccgg ccctcgcgga
     2941 cgatgaatgc tcgcccggac gcgagcctgt cggtggtcat tcgggatctc catggaccgc
     3001 tgccgcgtcg acatccgcgg cgtagacacc gcgctggcgc ggcgggagca gctcggcgat
     3061 gcgggtcatg atcaggcggg tggcggcctc ggtggccgcc cggcgatcct tcggggcgcc
     3121 cgcgccggtc cgctccagct cttcgccgac cacgaacggc ttgccgtagc ggaccgtcac
     3181 gtggcgcccg aagtgcggca ggagctggcc gcgcggccac atgaggtcgc tgtcgacgac
     3241 ggcaactggc agcaccgtcg cgccgctccg aagcgcgagc atgccgacgc cctcgcgcac
     3301 ctgctggagc ccgccgtcgc ggctgcgcgt gccttctgga aacacggcaa gcacctggcc
     3361 ggcttcgagc acccgcatcg ccgagcggaa cgcctcgagg tcggccgcgt cgcgatcaac
     3421 cgggtggatg ccgccgaggc gcatcgccca gcccgtcagt gggaactcga ccagctcacg
     3481 cttgccgagc cagttgagcg gtcgatcgat gatctggccg aggaacgcac cgatcagcac
     3541 cggatcagcg ctcgacgcgt ggttggcggc aacgatcacc gggcctctcc tcggcaccgc
     3601 atcgaggtca ccttcgacct tcattcggac aactactcgc gcgatcgttc ggaacagcag
     3661 gctcgcgaga ctgatgatcc agttcatccg ggtcgcgacc ggcgtctggc ggatcggccg
     3721 gcgcttcgcg cgcggcgcgc gcgggtcacg cttcggcgtt gggtccgctg cgtccccggc
     3781 ctggcccttg gccgcgtgcg ccgtctctgc ggtgcgaatg gcgtcgacga ccaggcgaac
     3841 ggtctgttcg aacgcgttgc catcggttcg gatgacgacc gcgtcgtcgg cggcacgaag
     3901 cggagcgatc gggcggctcg agtcgagctg gtcgcggcgc ttgagctcgg cgacgatttc
     3961 ggcggcctcc ggcgaagccg gatcgatgcc gcgttcgagc acccgccggt gtgcccgctc
     4021 ctcgaccgag gcgtcgagga acagcttcag gtcggcatcc ggcagcacta ccgtgccggt
     4081 gtcccgcccg gccatcacga tgctgcctgc gctcgcgagc cgccgctgcc gggcgaggat
     4141 ggccaggcgc agctcaccga tgccggagat cgccgagacc gcagcgtcca cgtcgggccc
     4201 gcgcacctcg gccgttcgat cgacgccgtc gaccgagacg tggcccaggc gtcccccacc
     4261 atcgtcgacc agctcgatct cgtcgaccag ggcaacgagg ccggtggcgt cgttcgcggg
     4321 cacgcggcga tgcagcgcga gccacgtgac ggcgcggtac aacaggccgg aatcacagaa
     4381 ccggtagccg agctcggcgg ccgccgcagc gccgacgctg ctcttgcccg aggacgccgg
     4441 gccatcgagg gcgacgacga gctgccggct cgcagagggg gttgacactc ggccggatgg
     4501 tacgcgacgg cgcttccgat tgatgcgccg gcaccgggct cgcccggccg gcgccgcgtc
     4561 agttgtaggt gtacgaggaa cccgcgtgcg gtagcgagct gcccccgtcg ttgttgacga
     4621 tgacctggac cgacgagccg ttggcgccac tcggggagtt gacgacgagc tgcgtgtcgt
     4681 tgacgaccac gaacggaacg ctcgcgccgc cgaacgtgac cgacgtcgtc ccggtgaagt
     4741 acttgccatc gacggtgacc gtcgtcccgc cgacacgggt accaacgctc ggcgtgaggc
     4801 tcgagacgta cggcggcggg ctgagctgga tgagatgtgt ccggatgccc gcgaactggg
     4861 tcgcgatgac gctgaacacc tccgccaggt ccgacgtctt cggctggcag aagaagtggt
     4921 ccccgtccgt gttctccgcc gcgatgcagc cgttggcggt gccgttcggc gacggcgcct
     4981 ggttggtcga cgccatggcc gcgagcgcgc gcgtcacgtt ctggccggag tacgtgcccg
     5041 aggagtccgg gcagtcagga tccgagccgg agtcgaagaa gccgaccgtg aacagctcga
     5101 tgttctcgcc gttggcgttc gtgatcgcct tggcggcgga ggccgccgca accgccgatg
     5161 cacaggtgta gttgcccggg tcgccggtgc tgccgtagct cggctggcca tccgtctcga
     5221 acaggatgcc ccacttgacg ttcggccggc cgtactgctg gaggtaggcc gcggccatgg
     5281 cgatcggcgt cgtcaggttc gtcccggtac cgcccggatg atcgaagcag ttgatcgccg
     5341 acacgatgtg ggtgccgttc tggagggtgc cgctgccatt gacgtatgcc tcgttccagg
     5401 cgggcgccgg agtgtccgaa tcagtcccgg tgaacccgat cgggatccac ttggcacggt
     5461 ctgccgggta cgtggtcccg tagtggttgc tgacatcggg gctgagcgcc acgtgttggg
     5521 ccgcgtacga gcccccgccg gcaaccgtgg cggtcgtggc gccggtggcg ccgacgctcg
     5581 cgacggtctt gctcgccgct gcgatcgacg gtcctgccgt gtttccgccg cgccggtcga
     5641 accgctcggt cgtgctgttc gtccaggtgc cgaagtagtc cgtcgcggtt ccgccggatc
     5701 cgttggccat cgccaggaag gcgacgaggg cgacattggc gcgggtcgcg gtgatggcgt
     5761 tggccgtggc gttgatcgtc cccgacacgt cactgcccgt attgccgcca tcggcgttga
     5821 tcgggttgac gttgtccaca ccggtgtagc gggcgatgcc gccggtcgcg cggaccgtca
     5881 tcaggacgcc ggtcgacgcg ttcctgaacg tccactggta ggtgcccggc tcggacgcgg
     5941 tgccgacctt gtagaaggtc gcgagcccga ccgagctgcc gttgttcgtc gttcggatgc
     6001 tggtccagcc ggctggcgcc acgatctgga cgttgcccga cccgccggtc aggatcgaat
     6061 tgttgaccgt gatcccggcg atgaggacat gccctgccgc gctggcggcc ccgggacgat
     6121 tgatggtcag gctcgaggcg cccgccaggg gcgtcgccgc ggagtcggag ctgctgaacg
     6181 ccggcgcgct cggtgcggag ctgtcgatgg cattgacgct gactgcgggg ccgccggtgc
     6241 cgttgcacga ggtgtttggt tcgcttgggc cgaggaagcc gagcgccacg cgctggagcg
     6301 ccgggtcgta ctcggagagg atcgacctcg tcgcgtcgcg ggcgttgacg atctgcgggt
     6361 ccgtcatcga tgccgtgcgg tccacgagga cgacgaggtc aaccggcgcg gccggcgact
     6421 gaccacacgg cccgttgcac gcagcggaaa ccgtcgtccc ggtgtagccc tcgttgatgc
     6481 cgacgacgcg gccgaacgtg tagctcgtgg tcgcctggcc ggtgatgacg acgacgttgc
     6541 acatgtcgcc gagggtgggg tcgcagacgc tgtagcgcgt cgcgccggca cccttgaagt
     6601 ctgtcgcgac cggggtgtgg ccgagcgccc cgcgtgggtt gcacaccagg ggaacgtccc
     6661 gcgagatata cggcgcgggc ggactcgcgc tgttgatgcc gatcaggcac cgatagctga
     6721 tcgtgtagtt ggccggtgcg atgcccggat aggtggcctg gagcgtctgg atgatgaggt
     6781 cccggacgga ctgccaggcc acggtgttga tggacgtgca ggcggacgcg gtggactgcg
     6841 cgatgttgca ggcatccacg ggcatcttgg tgcccccggc gagcgccgcc gaatcaacgg
     6901 cgttcaccag gatctggcgg ttgttccgga gcaggccgac atcgacgacg atcgacgcga
     6961 acagcatgat cacgacgatg accatcgcga acaggaccag gatctggcct tcctggggtc
     7021 ggtcccgacc gtcgacgcgc tgtgccctca attgctgtcc ccccggaccg ctcgcatgcg
     7081 agcgttgcgt cactgtggta cctccaggac catctggacc tgcgtgccca acggcacggt
     7141 cgagccaaac agctgcgaga agaaggatcg atacgtgtag gtgaccgcca ctcggatcga
     7201 atcgccggcc tccgcgtcct cgacaccacc ggccgcgaag tcgcacgcgt tgtgatttgc
     7261 caggttcggg atgcaggcga tggtcgagga caggtccgac caggtgaggc cggtcaggtt
     7321 cgcgccgatg gccccggtct ggctgttcag gtcacccggg atggcggtga cgttgctctg
     7381 gtagaccgcc cagcgagccc cctcgcgcgc ggcggagatc accgtcatgc gcgaatagag
     7441 caggaacccg aagtcgagga tgcccgccag cacgaccagc aggatcggga agatcagggc
     7501 gaactcgacg agcgactggc cgcggcggtt gctcgcgcgg cgacccgccg gggatccgag
     7561 gccgtggcgc atcactcgag gaccatcctg acgtccgtgc tcaggttgac cgtcgtgccg
     7621 aggaagcgac cgaacatcga tggataggca taagacgtcc gcacgcgcac ggagtcgccg
     7681 gtctgggcat cgcgcgtggc gttgccgccg gtgaagtcgc aggcggagag gccggccgcc
     7741 ggcacgcagg tcgtcgtgat cgtgagaccc gcctggacga tgccgggggc gttcgcgcgg
     7801 atgggaccgc ccggcgaatc gaggttctca tcgatcgcca cgctgttgtc gctctggacc
     7861 gtggccgcgt gcgcgccctc gcgcgtggcg ttgatcaggg tgagtcgcga gaacgccatg
     7921 aacccgaagt cgaagatcgc gaacaggatc atgaggaaga ccgggatgac aagcgcaaat
     7981 tcgaccaacg actgaccgtt ggcctgatga tcacgtgcgc ggccgaccgc cccccgttgc
     8041 gacatggcca cagattgccc tgtgccaatc gaagggacaa tccggcctaa gtacccctat
     8101 ccgacacggg tagtacgttc ccggtgcgcc ggtgcaacgg gaccattgcg tcacggccgg
     8161 ggcggacctt agtcaccggc ccctggcttc ctccgacgcg gcgctcgcga gcgctcattc
     8221 ggcttctcga ctcggtcacg ctggacgttc ctgatggcga gcgcggcccc ggttgcgaac
     8281 agctcgagga gcgcctggtc agcgggatcc cagtcgtgtg ccgggcggag caacccgatg
     8341 agcgtgccga agccggagga gccgaggtcc agggtggcgc gaacccggat cggttcaccg
     8401 ggcacgtgat cggtgtcggg cagcacgttg ggctgcccga gcacgatttc ggcctcgatc
     8461 atgtcgaacg cccggcgagc gcccgctgcg gcggccgtga ccattcccat cgaatcggcg
     8521 gtcggatcga tcgcggcgat ctcggcccgt acggcatcga gcgcaccgct ccgccggtcc
     8581 aactccgacg tgagctggtt gtagcggtcg gcgaggcgcg cgagctcgtc ctcacccgcc
     8641 acctcgaccc gcgccggccg ctcgccggtc cccg
//
LOCUS       DATBSW010000283         9972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_802402, whole genome shotgun sequence.
ACCESSION   DATBSW010000283 DATBSW010000000
VERSION     DATBSW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802402"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2943)
                     /gene="polA"
                     /locus_tag="VIF63_07920"
     CDS             complement(<1..2943)
                     /gene="polA"
                     /locus_tag="VIF63_07920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY8239349.1"
                     /translation="MERLMLLDGFGLVYRGYYALPPLTTSKGELVNGVFGFCSIVLRG
                     FADLKPDYVAVCFDLSGPTFRHEQYAEYKATRTRMPDDLAAQFPKVREVVKALRIPVY
                     ELQGFEADDVIGALTVQAEAAGVETTIVTVDLDMLQLVSDKTRLMTTRSGVENTVIYD
                     PARIWERYGLRPDQMIDYKALKGDPTDNIPGVPGVGEKTAAKLIAEWGTLDNMYTRIA
                     DVKPEKLRDKLIEHRDAVYRGKDLTTIVRDIPEISLDLEAARLADYDRETVIRLFREY
                     EFRTLIERLPAMSGESAVEKVQALREISSSGTVGAARVGTERPAGWGSGRPIRSGSLG
                     GDSLQLSLDFDTVSQGGGAAARGGTAPGDGAAGEAGVGGGIAAPERFDDLSTALAATI
                     NNPALIGVLDAADLPGVEAWLAEQTTVGASIVSDDPRPRRGTAQAFAVAGRDGRVVVG
                     FGAEAADGLRHMVEARDIPIVAHEAKPILVPRFAADAAAPATPVEFDTQIGAYILNAA
                     LRSQSIADVVAERLDLVLPPPKELEPVHRAGLEALSALAVRDPLARALQEEGLERLFR
                     EIELPLIPVLARMEADGIGVDLEALAVLDREFGIEITRLEEAIYGAVGHQFTIGSPKQ
                     LGEILFEELKLPKGRRTKTGYSTDATVLEELAGVHPVIQPVLDWRIYTKLRSTYVEAL
                     PTLIADDGRLHTTFHQAVAATGRLSSSDPNLQNIPIRTPLGRRIRRAFVADTPETTLI
                     AADYSQIELRIIAHVADDVALKAAFERKADIHRETAATVLGKDPSEITPDERSMAKMV
                     NFGLAYGMSDFGLSTRAGISRQEAQAFINAYFAAYSGISYYMLHIKDTARRQGFVTTL
                     LGRKRSIPELHAANAALRGAGERMAINMPIQGTAADIVKIAMIRMADRLRDGGFKARM
                     LLQVHDELLLEAPLDEVDRLVPLLRETMEGALPLSVPLTVDVKVGRDWESMTPVSRLD
                     AALAEADEP"
     gene            complement(3025..4566)
                     /locus_tag="VIF63_07925"
     CDS             complement(3025..4566)
                     /locus_tag="VIF63_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8239350.1"
                     /translation="MSGLRRLGPAITAGLLVATLLPGTASAAAYDPASDMNSMYSTAQ
                     YVGATAYWDAGFTGAGVDVALIDTGVSPVEGLTTPGKVINGPDLSFESQNPSFRYLDT
                     NGHGTFMAGIIAGKDEALAAPYSAAPASVYRGIAPDARIVSVKVGVADGGVDVSQVIA
                     AVNWVVEHRQDNGLNIRVLNISYGTNSTQPYEVDPLAFAVEQAWKNGIFVVAATGNSG
                     YVSRHGTLTSPAYDPKIFAVGASDSMGTATLDDDTVPAFSSTGSRDRSPDVVAPGTHI
                     VALRVPGSYVDQCYASTGFVSDRYFRGSGTSAAAAVMSGEAALVIQQRPSITPNQLKK
                     LFVSTAQRMASLSNRYQGAGVVDLRETLTARTLNSPARWKTIDWSTGTGSLEKARGSD
                     HLTRDGVVLTGEQDIFGHSFNAVEIATLAAAGSSWSDGIWNGSSWSGSSWSGSSWSGS
                     TWSGSSWSGSSWSSSSWSDSTWNGSSWSGSTWSGSTWSGSTWSGSTWSGSTWSDSSWS
                     TGSWE"
     regulatory      complement(4700..4789)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4901..7573)
                     /locus_tag="VIF63_07930"
     CDS             complement(4901..7573)
                     /locus_tag="VIF63_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8239351.1"
                     /translation="MLGQYSRSANALVARLNAAMSGGSRVWAFAAVLGSLTIVLWPFL
                     PASNSVAAPFGIPWWLLAVGFFVAEANVIHLHIGRSAHSFSMSEIPLVVGLVLVAPHE
                     FVLARLLGSGLALYFARRQRSVKLAFNLAQFGISSLAALGVVRLFSLADHGFGPVLWA
                     GVLTATLAENLVGVMTVSTAISLAEGRSMRARLPEMLKIGVIVSMTNASLALMGLVVL
                     WTNPGAVWLFAVPIVTAAIAYRAYIAERQQHERVELLYESTRILQRNPRLDAAIMALL
                     EHLRRMFRADVAEICLIPRREGDDILRSRLGPGTATEVMRVIGPSLDDSRLVTAVAER
                     KASLVNPAVAESEGGSSESFRSALVAPLLGESNLVGTILVSDRLSEISEFDLDDLKLF
                     ETLANHAAVALENGQLEQSVERLSQLKEELHYQASHDALTGLANRTVFAQSVAQRLES
                     AQADGTIPVVLFIDLDDFKVINDTLGHAAGDRLLVAVGDRLRSVVRATDLAARLGGDE
                     FAALLWDTPDLSSSLRVAERMVAAMNPAFDLGGNPITVRASVGVAGGRPGVDTAGDLL
                     RNADVAMYSAKARGKGRVILFETSMHEAVLAHAALSADLERAIANREFILQYQPIVEI
                     KTGRMIGVEALVRWRHPIRGVIEPTDFILLAEESDAILDIGRWVLDEALAQARAWRTV
                     NPSEPFTVSVNVSARQLAQHTFVDDVIGAVADAGVEPHSLVLEMTETAMLHDSAATRQ
                     RLQAFKEAGIGISVDDFGTGYSSLSYLQRFPVSALKIARDFVELEPDDHDAWQFASAI
                     IAMADALHLSVIAEGVERSEQLRRLSELGCDYAQGFFFARPLDAAAITSMLLHGRSFE
                     KLAGPAPAKKAPATVRRASSPPVPRPVVRPASTG"
     gene            complement(7760..8572)
                     /locus_tag="VIF63_07935"
     CDS             complement(7760..8572)
                     /locus_tag="VIF63_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239352.1"
                     /translation="MHHSNHADNPNHASHDTFLIAGHAAGDLVDSERTRAQALLAECS
                     SCADLHRDLVAIAGATRALPNLATAPRDFRLAPEQAARLRRGSWLRAALAPFGAARSA
                     TRPLAAAFTSLGICGLLIGSALPGLLGGPAALAPAIAPAIAPERDLGTGAAAAPSQAP
                     QAVPAAGGPKASSGTPDRAGGEYSATGAPLFGSDNGGPKSTGGPTSTNYDAIVAGAVD
                     QSQAPADGTFGKGTEFLPSAPPPNLLVVGSLGLLAIGLALFALRLAAGRLRA"
     gene            complement(8614..9354)
                     /locus_tag="VIF63_07940"
     CDS             complement(8614..9354)
                     /locus_tag="VIF63_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239353.1"
                     /translation="MADLGHDRDEATEAALAVAARHALHDEELIAGFAVDGLDDDADI
                     AKAKSLVERCAACRDLFVDVQAISGALRVEARRTIAAPRDFQLSVDDARRLGGTISPR
                     GFVRNLRRAMVSFGRPIGASMAALGIVGVLVGSFSFGGLGAAGAPTAGNPGPGAAATD
                     AAIKEQTGPESAAPGATTDSTGFGPYATELMSAADEAQRDLARLSAPGGAGVLFAGSI
                     GLLIVGLSLAWYATRRTDRKRLDPSRFH"
     gene            complement(9347..>9972)
                     /locus_tag="VIF63_07945"
     CDS             complement(9347..>9972)
                     /locus_tag="VIF63_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8239354.1"
                     /translation="RTPPIVEDLRSTTVPDDPVQRRDLILLERARAGDLDAFNDLVVV
                     YQDLLFALIVRMVPDRDQASDAVQEAFFSAFRNLDGFRGGSVKSWLSRIAINAAMDTQ
                     RLKKRRPADPYPELEDDTWQPPADASADPVTTSLTSERHRALNQALSQITNDQRAAIV
                     LYDVEGYDYAEIAEMTSVSVGTVKSRIHRGRLALRGLLLDRMDLFRG"
BASE COUNT         1514 a         3354 c         3564 g         1540 t
ORIGIN      
        1 cggctcatcg gcctcggcga gcgcggcatc gagccgcgac accggcgtca tcgactccca
       61 gtcgcggcca accttgacgt cgaccgtgag cggcacggac agcggcagcg cgccctccat
      121 cgtctcgcgc agcaggggca cgagccggtc gacctcgtcc agcggcgcct cgagcaggag
      181 ctcgtcgtgg acctggagga gcatccgggc cttgaacccg ccgtcccgca ggcggtcggc
      241 catccggatc atcgcgatct tcacgatgtc cgcagccgtg ccctggatcg gcatgttgat
      301 cgccatgcgc tcgcccgcgc cgcgcaaagc ggcgttggcc gcgtgcagct cgggaattga
      361 ccgcttccgg ccaagcagcg tcgtgacgaa cccctggcgc cgggccgtgt ccttgatgtg
      421 gagcatgtag tagctgatgc cggagtacgc cgcgaagtac gcgttgatga acgcctgcgc
      481 ctcctggcgg ctgatgccgg cgcgcgtcga gagcccgaag tcgctcatcc cgtaggcaag
      541 cccgaagttg accatcttgg ccatcgaacg ctcgtccggc gtgatctccg aggggtcctt
      601 gccgagcacc gtggccgcgg tctcgcgatg gatgtccgcc ttgcgctcga acgccgcctt
      661 gagggccacg tcgtcggcaa cgtgggcaat gatccgcagc tcgatctgcg agtagtccgc
      721 ggcgatgagc gtggtctccg gcgtgtccgc gacgaacgcc cggcggatgc gccggcccaa
      781 cggcgtcctg atcgggatgt tctggaggtt cgggtcggac gatgacagcc ggcccgtcgc
      841 ggccaccgcc tggtggaacg tcgtgtgcag gcgcccgtcg tcggcgatca gggtcggcag
      901 cgcctcgacg taggtcgagc gcagcttcgt gtagatccgc cagtcgagca ccggctggat
      961 caccggatgg acgccggcga gctcctcgag cacggtcgcg tccgtcgagt agccggtctt
     1021 cgtccgtcgg cccttgggca gcttcagctc ctcgaacagg atctcgccga gctgcttggg
     1081 cgagccgatc gtgaactgat gcccgaccgc gccgtagatc gcctcctcga ggcgggtgat
     1141 ctcgatcccg aactcgcgat cgagcacggc gagcgcctcg aggtcgaccc cgatcccgtc
     1201 cgcctccatc cgtgccagca ccgggatcag cggcagctcg atctcgcgaa agaggcgctc
     1261 gaggccctcc tcctgcagcg ccctggcaag cgggtcgcgg acggcgagcg ccgacaatgc
     1321 ctccagcccc gcccgatgga ccggctcgag ctccttgggc ggcggcagga caaggtcgag
     1381 ccgctccgcg acgacgtcgg cgatcgactg cgatcgaagc gccgcgttga ggatgtacgc
     1441 gccgatctgc gtatcgaatt cgacgggcgt tgccggggcg gcggcatcgg cggcgaaccg
     1501 cgggacgagg atcggcttcg cctcgtgggc cacgatcggg atgtcgcgcg cctcgaccat
     1561 atgccggagg ccgtcggctg cctcggcccc gaacccgacg accacccgac cgtcgcggcc
     1621 ggcgacggca aacgcctggg cggtcccccg ccgcggtcgc gggtcgtcgg acacgatcga
     1681 ggcgccaacc gtggtctgtt ccgcgagcca ggcctcgacg cccggcaggt cggctgcatc
     1741 gaggacgccg atcagcgccg ggttgttgat cgtcgccgcg agcgcggtgg acaggtcgtc
     1801 gaagcgctcg ggcgcggcga tcccgccacc cacgccggcc tcgccggcgg cgccgtcacc
     1861 gggcgcagtc ccgccgcgcg ccgccgctcc tccgccctgg gagacagtgt cgaagtccag
     1921 cgacagctgc aggctgtcgc caccgagcga gcccgaccgg atcggccgcc ccgagcccca
     1981 ccccgccggc cgttccgtcc ccacccgcgc cgccccgacc gtcccgctcg acgagatctc
     2041 gcggagcgcc tggaccttct cgaccgcgct ctcgccggac attgccggca gccgctcgat
     2101 cagggtccgg aactcgtact cgcggaagag ccggatcacc gtttcgcggt cgtagtcggc
     2161 gagccgggcg gcctcgaggt cgaggctgat ctccggaatg tctcgcacga tcgtcgtcag
     2221 gtccttgccg cggtagaccg cgtcgcgatg ctcgatgagc ttgtcgcgca gcttctccgg
     2281 cttcacgtcg gcgatgcggg tgtacatgtt gtcgagcgtg ccccactcgg cgatgagctt
     2341 cgcggctgtc ttctccccca ccccgggcac gcccgggatg ttgtcggtcg ggtcgccttt
     2401 gagcgccttg taatcgatca tctggtccgg tcgaaggccg taccgctccc agatccgggc
     2461 cgggtcgtag atgaccgtgt tctcgacccc tgagcgcgtc gtcatcaggc gcgtcttgtc
     2521 gctgacgagc tggagcatgt cgaggtcgac cgtgacgatc gtggtctcga cgccggccgc
     2581 ctcggcctgg acggtcaacg caccgatgac gtcgtcggct tcgaagccct gcagctcgta
     2641 gacggggatg cgcagcgcct tgaccacctc gcgcaccttc gggaactgcg cggcgaggtc
     2701 gtccggcatg cgcgtgcggg tggccttgta ctcggcgtac tgctcgtggc ggaaggtcgg
     2761 ccccgacagg tcgaagcaga cggcgacgta atcgggcttc aggtccgcga agccgcgcag
     2821 cacgatgctg cagaagccga acacgccgtt gaccagctca cccttcgaag tcgtgagtgg
     2881 cgggagggcg tagtagccgc ggtacacgag cccgaagccg tcgagcagca tcaggcgttc
     2941 catcagggaa cgagtgtatc gagggcggga cctcagcctc gcgcgctcgc tgcccctccg
     3001 ggggcccggt tcagggacgc gtgatcattc ccacgagccg gtcgaccagc tcgaatcgct
     3061 ccacgtcgag ccggaccacg tgctccccga ccacgtgctc cccgaccagg tgctccccga
     3121 ccacgtgctc ccggaccagc tgctgccgtt ccacgtcgag tcggaccagc tcgagctgga
     3181 ccagctcgag cccgaccagc tgctgccgga ccacgtgctc cccgaccagc tcgacccgga
     3241 ccagctgctg ccggaccagc tgctgccgtt ccagatgccg tcggaccagc tgctcccggc
     3301 ggccgcgagg gtcgcgattt caaccgcatt gaagctgtgg ccgaagatgt cctgctcacc
     3361 cgtcaacacg acgccgtcac gggtcagatg gtcggatccc cgcgccttct cgagcgaccc
     3421 cgttccggtg gaccaatcga tggttttcca tctcgccggc gagttgagtg tccgcgccgt
     3481 gagcgtctcg cggaggtcga cgacccccgc gccctggtag cggttcgaga ggctggccat
     3541 ccgctgggcg gtgctcacga acagcttctt gagctggttc ggggtgatcg acgggcgctg
     3601 ctggatcacg agggcggcct cgccggacat cacggcggcc gcggcgctcg tgccgctgcc
     3661 acggaagtag cgatcgctga cgaacccggt cgaggcgtag cactggtcga cgtacgaccc
     3721 tgggacgcgg agcgccacga tgtgcgtgcc cggtgcaacc acgtccggag accgatcgcg
     3781 gctgccggtc gaagagaagg ccggcaccgt gtcatcgtcg agtgttgccg tgcccatcga
     3841 gtccgaggcg ccgactgcga agatcttggg gtcgtaggcg gggctcgtga gcgtgccgtg
     3901 tctggatacg tagccgctgt tgcccgttgc ggcaacgacg aagatgccgt tcttccaagc
     3961 ctgctcgacc gcgaaggcga ggggatccac ttcgtacggc tgcgtcgaat tcgtgccgta
     4021 gctgatgttc aggacccgga tgttcaggcc gttgtcctgg cggtgctcga caacccagtt
     4081 gaccgcggcg atgacctggc tcacgtcgac gccgccgtcg gccacgccga ccttgacgct
     4141 gacgatccgc gcgtccggcg cgatgccgcg gtagaccgag gcgggtgcgg cgctgtacgg
     4201 ggcggcaagc gcttcgtcct tgccggcgat gatgccggcc atgaacgtcc cgtggccatt
     4261 cgtgtcgaga taccggaatg acgggttctg cgactcgaac gacaggtccg gcccgttgat
     4321 gaccttgccg ggggtcgtca ggccttcgac cggggagacg cccgtgtcga tgagcgcgac
     4381 gtcgacgccg gcgccggtga accccgcgtc ccagtaggcg gttgccccga cgtactgcgc
     4441 ggtgctgtac atcgagttca tgtcggatgc agggtcgtac gcagctgcgg acgcggttcc
     4501 aggcaggagg gtggccacga gcaggcccgc ggtgatcgcg ggaccgaggc gacggaggcc
     4561 gctcacgaga ggctccagcc gcgcaggctc cagccgcgca ggctccagcc gcgctcaccc
     4621 tgcggagggt gcgtgcggtc cggcgagcga tccacctgca tcatcgattg cgcctcgttg
     4681 gcagactcga tgtgcggtgc ctggtaaccg ggctgccttt gggaggttca ctcccggaag
     4741 gtccctggct ttgcgtcccc gcctctcgac gggtttgccg tgtttcagac ctcactccgg
     4801 cagcccactg ctgccagttg tcccgggacc gtacggttac cccttgcgac gacccatagg
     4861 acgaaagtcc tgtttggtgc gcgcgggaac cagatgcgtc tcagcccgtg ctggccggcc
     4921 tcacgactgg acgcggcacc ggcggcgagc tggcgcggcg cacggtcgcc ggggccttct
     4981 ttgcgggggc cggcccggcg agcttctcga acgaacggcc gtggagcagc atcgacgtga
     5041 tcgccgccgc gtcgagcggg cgggcgaaaa agaacccctg ggcatagtcg cagccgagct
     5101 cggacaggcg ccggagctgt tccgaccgct cgacaccctc ggcaatgacc gagaggtgca
     5161 gcgcatcggc catggcgatg atggcgctcg cgaactgcca ggcgtcgtga tcgtcgggct
     5221 ccagctcgac gaagtcgcgg gcgatcttga gcgccgatac cgggaagcgc tgcagatagc
     5281 tgagcgatga atagccggtg ccgaagtcgt cgacggagat gccgatcccg gcctccttga
     5341 acgcctggag gcgctgccgg gtcgcggcac tgtcgtggag catcgcggtc tcggtcatct
     5401 ccaggaccag cgagtgcggc tcgacgccgg cgtcggcgac cgcgccgatg acgtcgtcca
     5461 cgaaggtgtg ctgtgcaagc tgacgcgccg agacgttgac gctcaccgtg aacggctccg
     5521 acgggttgac cgtgcgccag gcacgcgcct gtgccagggc ctcgtcgagc acccatcgac
     5581 cgatgtcgag gatcgcgtcc gattcctcgg cgaggaggat gaagtcggtc ggttcgatga
     5641 cgccgcggat ggggtgtcgc cagcggacga gcgcctcgac cccgatcatc cggccggtct
     5701 tgatctcgac gatcggctgg tactggagga tgaactcgcg gttcgcgatg gcccgctcga
     5761 ggtctgccga gagcgccgca tgcgcgagca ccgcctcgtg catcgatgtc tcgaagagga
     5821 tgacgcgacc cttgcctcgg gccttggccg agtacatggc cacgtccgcg ttcctgagca
     5881 ggtcacccgc cgtgtcgacg ccgggtcggc cgccggccac gccgacgctg gcccgcaccg
     5941 tgatcgggtt cccgccaagg tcgaacgccg gattcatcgc tgcgaccatt cgctcggcga
     6001 cccgcagcga gctgctgagg tcgggcgtgt cccagaggag cgccgcgaat tcatcgccgc
     6061 cgagacgagc ggcgaggtcg gtcgcgcgca cgaccgagcg gaggcgatcg ccgacggcca
     6121 cgaggagcct gtcaccggcg gcatgaccca gggtgtcgtt gatcaccttg aagtcatcga
     6181 ggtcgatgaa cagcaccacc gggatcgttc cgtcggcctg ggccgattcg aggcgctggg
     6241 cgacggactg ggcgaagacg gttcggttgg cgagcccggt gagggcatcg tgggaggcct
     6301 ggtagtgcag ctcctccttg agctggctca ggcgctcgac ggactgctcg agctgcccgt
     6361 tctcgagggc caccgccgcg tggttcgcca gcgtctcgaa cagcttgagg tcgtcaaggt
     6421 cgaactcgct gatctcgctg agccggtccg agacgagaat cgtcccgacg aggttggact
     6481 cgccgagcag cggcgcaacc agcgcgcttc ggaagctctc ggagctacct ccctccgatt
     6541 cggcgacggc cgggttgacg agactcgcct tgcgttccgc gaccgcggtc accaggcgcg
     6601 aatcgtcgag cgacggcccg atgacccgca tcacttcggt tgccgttccc ggaccgagcc
     6661 gtgaccggag gatgtcgtcg ccctcgcggc gtgggatcag gcagatctcc gcgacatcgg
     6721 cgcggaacat gcgccggaga tgctccagga gcgccatgat cgcggcgtcc agtcgcgggt
     6781 tgcgctggag gatccgtgtt gactcgtaga gcagctcgac ccgttcatgc tgctgccgct
     6841 cggcgatgta ggcgcgatac gcgatcgcgg cggtgacgat cggcacggcg aagagccaca
     6901 ccgcacccgg gttggtccag aggaccacca ggcccatcag cgcgaggctc gcgttggtca
     6961 tcgagacgat gacaccgatc ttgagcatct cgggcagccg tgcgcgcatc gagcggccct
     7021 cggcgaggga gatcgcggtg ctgacggtca tcacgccgac gaggttctcc gccagggtcg
     7081 cggtgaggac gccggcccac agcaccggcc caaaaccatg atctgcgagg gagaacaacc
     7141 tgaccacacc cagcgcggcg agggacgaga tgccgaactg cgcgaggttg aacgcgagct
     7201 tcactgaccg ctgccggcgc gcgaagtaca gcgcgaggcc cgagccgagc agccgtgcca
     7261 gcacgaactc gtgcggtgcg accagcacga ggccgacgac gagagggatc tcgctcatcg
     7321 agaaggagtg ggcgctccgt ccgatgtgga gatgaatgac gtttgcctcg gcgacgaaga
     7381 acccgactgc cagcagccac caggggatgc caaacggggc agcgaccgag ttgcttgccg
     7441 gcaggaatgg ccagaggacg atcgtcaggg acccgagcac ggccgcaaag gcccagaccc
     7501 gtgagccgcc ggacatggcg gcattgagtc gggcgaccag tgcattcgcc gatcgcgagt
     7561 actgacccaa catgcatcgt ctcgatatcc agccgtcacg agcggatgcc cgggatacgg
     7621 cttgggccga catcgaccgt acggtgggac agtgggagcg gcccactacc cgttgggtcg
     7681 agtggactag tacggatggt gcatcggcgc acccatttct tgcgatgcgc gagccgacct
     7741 cgcgagcgcg gatcgggcgc taggcgcgga ggcggcctgc ggcgagccga agcgcgaaca
     7801 gcgcgaggcc gattgcgagg agcccgagcg agccgacgac gaggaggttc gggggcggcg
     7861 cggacggcag gaactcggtg cccttgccga aggtgccatc ggccggggcc tggctctggt
     7921 caacggcgcc ggcaacgatt gcgtcgtagt tcgtcgaggt cggcccgccg gtcgacttcg
     7981 gcccgccgtt gtccgatccg aaaagcggag cgccggtcgc ggagtattcg ccgccggctc
     8041 gatcgggagt gccgctggat gccttggggc cgccggccgc cggaacggcc tgcggagcct
     8101 ggctcggtgc cgcagcggcg cccgtcccca agtcccgctc gggcgcgatc gccggcgcga
     8161 tcgccggcgc gagcgccgct ggcccgccga ggaggcccgg aagggcgctg ccgatcaaca
     8221 gcccacagat gccgagcgac gtgaacgcag cggcgagagg ccgggtagcg gaccgcgcgg
     8281 ccccgaatgg cgcgagcgcg gcacgaagcc aggagccgcg acggaggcgc gctgcctgct
     8341 cgggtgcgag ccggaagtcg cgcggtgcgg tggcgagatt cgggagggcg cgggtcgcgc
     8401 cggcgatcgc gacgaggtcg cgatggaggt ccgcgcagct cgagcactcg gcgaggaggg
     8461 cctgggcccg ggtgcgctcg gaatcaacga ggtcgccggc cgcgtggccg gcgatcagga
     8521 aggtgtcgtg ggatgcgtgg ttcgggttgt cggcgtggtt cgaatggtgc atctcgccgt
     8581 ttggacgagc gtccggagcg attggttccc tcgtcaatga aatcgcgagg ggtcgaggcg
     8641 cttccggtcc gttcggcggg tcgcgtacca cgcgaggctg agtccgacga tcaacagccc
     8701 aatcgacccc gcgaagagga ctccggcgcc gcccggcgcg ctcaggcgag ccaggtcgcg
     8761 ctgcgcttca tccgccgccg acatgagttc ggttgcgtag gggccgaagc ctgttgaatc
     8821 ggtcgtcgcg ccgggcgctg cggactcagg gcctgtctgc tccttgatcg cggcgtcggt
     8881 cgcggctgct ccggggcctg gattgcccgc ggtcggcgcg ccggcggcgc cgaggccgcc
     8941 gaacgagaac gagccgacga gcacgccgac gatgccgagc gccgccatgg acgcgccgat
     9001 cggccgcccg aagctgacca tcgcccggcg caggttccgc acgaatccgc gcggcgagat
     9061 cgtgccgccg agccggcgcg cgtcgtcgac ggaaagctgg aagtcgcggg gtgcggcgat
     9121 cgtgcggcgc gcctcgacac gcagggctcc ggagatcgcc tgcacgtcca cgaacaggtc
     9181 gcggcacgcg gcgcatcgct cgacgagcga cttcgccttg gcgatgtcgg cgtcgtcgtc
     9241 gagcccgtcg accgcgaagc cggcgatcag ctcctcgtcg tgcagcgcgt ggcgcgctgc
     9301 gacagcgagc gctgcctcgg tggcttcgtc gcggtcgtgg ccgaggtcag ccacggaaca
     9361 ggtccattcg gtcgaggagc aggccgcgga gggcgaggcg tccgcggtgg atgcgcgact
     9421 tcaccgtgcc caccgacacg gacgtcatct cggcaatctc ggcgtagtcg tagccctcga
     9481 cgtcgtagag cacgatcgcg gcccgctggt cgttcgtgat ctgggacagc gcctggttga
     9541 gggcgcggtg ccgttctgag gtcagcgatg tggtcacggg gtcggccgag gcatccgcag
     9601 gcggctgcca ggtgtcgtcc tcgagctcgg gatatggatc ggccggccgg cgcttcttga
     9661 gccgctgcgt gtccatcgcg gcgttgatcg cgatccggct gagccacgac ttgacgctgc
     9721 cgccgcggaa tccgtcaagg tttcgaaacg ccgagaagaa cgcctcctgg acggcgtccg
     9781 acgcctggtc gcggtccggg accatccgca cgatgagcgc gaacagcagg tcctggtaga
     9841 cgacgacgag atcgttgaag gcatcgaggt cgcccgcgcg ggctcgctcg agcaggatca
     9901 ggtcgcggcg ctggacgggg tcgtcgggga ccgtcgtcga gcgaaggtcc tccacgatcg
     9961 ggggtgtcct cg
//
LOCUS       DATBSW010000284         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_27326, whole genome shotgun sequence.
ACCESSION   DATBSW010000284 DATBSW010000000
VERSION     DATBSW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27326"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1044
                     /locus_tag="VIF63_07950"
     CDS             106..1044
                     /locus_tag="VIF63_07950"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEY8239355.1"
                     /translation="MTVESLGRAVGLYRRTATSRSSIVLLVANLIPLVGVVFFGWSLL
                     TILVLYWVENGIVGFWNVPKIIMAQGSVIPTLPALPHSAAMAATGSAQAAADLEASWA
                     KARAAQEAQRQALSSTTGTVGSGGGPGSAFGRLASVGRIGMSMFFAFHYGMFWFVHGI
                     FVFALPAFGSGFGTGNDTPCSEGPLEPGSLPGSAIDLPLCANGVFGDIIWSNVLLAAG
                     VLFLSHGASFLFNYIGRGEYLTTSAARQMMSPYGRVVVLHITIIVGSIVAAFLGAPIG
                     ALIVLIGLKTAFDLGLHLREHGSSGPPLPSGVASAT"
     gene            complement(1067..1459)
                     /locus_tag="VIF63_07955"
     CDS             complement(1067..1459)
                     /locus_tag="VIF63_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239356.1"
                     /translation="MTENRFEAWLGRPEFESDEQKWLVGLASHPDDERLDVQSWLFNR
                     ILAIGIAYQLSYIQVIAAAAGSGASPDDDLPVEFDSVQSVALIEELEFVRLRINDPAV
                     DAVLDRLLLLAARCSRSPSETLRFTPIL"
     gene            complement(1456..2859)
                     /gene="hydA"
                     /locus_tag="VIF63_07960"
     CDS             complement(1456..2859)
                     /gene="hydA"
                     /locus_tag="VIF63_07960"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY8239357.1"
                     /translation="MTDLLVRGGTVVTAAGSRLADVAVSSGSIEAIEADLSGLTASAS
                     EVVDATGLLVLPGAVDVHTHTRVATDAEPDRFFQDSMAAAFGGTTTFLAFNNPGTGSP
                     RVAGRSLLAGVREWRAATEGDSAVDYALNPAISGRMDDPVAELPAMVDEGVATAKAFM
                     VFDFRLDERRIYEAMRVLGARGGMLQVHCEDPVLIDTAVADALARGDTAPQFHATTRS
                     TEAEAVATHRVMAFARAADVPVHVVHLSSAAALGHVRDAKARGVRAHAETCPHYLALT
                     DARYTSPDLASIAECVISPPLRPQSDQDALWAGLANGDLDLIATDHVPDRMAVEKAEA
                     VRGVSFDKVSNGAPGIETLLAIAYSEGVAKGRLSVERMVDVLSTSPARLFGLSRKGSI
                     EVGRDADLVLFDPAARRTLRAADLHHTSDYTPYEGFAVTGAVRSVFVRGRSVIRDGSF
                     VGTRGDGRFVERAGAGG"
BASE COUNT          442 a         1011 c         1019 g          467 t
ORIGIN      
        1 ccgggccgcc ggcaggctgg gcgaggccgc cgacctgctc gatcggctgg tcctcgatga
       61 cgacttcgtc gagttcctga ccctgcccgc gtacgatcga ctgcagtgac cgtcgaatcg
      121 ctcggccgcg cggtcggcct ctatcgccgc accgccacgt cgcggtcctc gatcgtcctg
      181 ctcgtcgcga acctcatccc gctcgtcggc gtggtgttct tcggctggag cctgctgacg
      241 atcctggtcc tgtactgggt cgagaacggc atcgtcggct tctggaacgt gcccaagatc
      301 atcatggccc agggctccgt gattccgacc ttgccggcgc tgccgcactc ggcggcaatg
      361 gccgcaacag gctcggcgca ggccgcggct gacctcgagg cgtcgtgggc gaaggcgcgc
      421 gcggcgcagg aggcgcagcg gcaggcgttg tcgagcacga cgggaacagt cggatcggga
      481 ggcgggccag gatcggcctt cggtcgactg gcgagcgtcg gccggatcgg catgtcgatg
      541 ttctttgcct tccactacgg catgttctgg ttcgtccacg gcatcttcgt gttcgcgctg
      601 ccggcgttcg gcagtggctt cgggactggc aacgacacgc cgtgcagcga gggcccgctc
      661 gaaccgggca gtctccccgg ctcagccatt gacctgccgc tctgtgcgaa cggcgtgttc
      721 ggcgacatca tctggtcgaa cgtgctgctc gcggccggcg tcctgttcct cagccacggc
      781 gcgtcgttcc tgttcaacta catcggccgc ggcgagtacc tcaccacctc ggcggcgcgc
      841 cagatgatgt cgccgtatgg ccgcgtcgtt gtgctccaca tcacgatcat cgtcggctcg
      901 atcgtcgcag ccttcctcgg cgccccgatc ggcgcgctga tcgtcctgat cggcctgaag
      961 accgccttcg accttggcct ccacctgcgc gagcacggct cgtcgggtcc acccctgccg
     1021 tcaggcgtcg ccagcgcgac ctgacctcgg caacacggcc gcgccgtcag aggatcggcg
     1081 tgaagcggag cgtctcgctg ggggagcgtg aacagcgggc ggccaagagc aggagtcggt
     1141 cgagcactgc gtccacggca ggatcattga tgcggagccg gacgaattcg agctcctcga
     1201 tcagggcgac ggactggacc gagtcgaatt cgaccggcag gtcgtcgtcg ggcgatgcgc
     1261 cggaaccggc cgcggcggcg atgacctgga tgtagctgag ctggtacgca atgccgatcg
     1321 ccaggatgcg gttgaagagc cagctttgga cgtcgaggcg ttcgtcgtcg ggatggctcg
     1381 cgaggccgac gagccatttc tgctcgtcgg actcgaattc cgggcgaccg agccacgcct
     1441 cgaatcggtt ttccgtcatc cgcctgcccc cgcgcgctcg acgaaccggc cgtccccgcg
     1501 cgtcccgacg aaggagccat cgcggatgac ggatctgccg cggacgaaga ccgagcgaac
     1561 ggcgccggtc accgcgaagc cctcgtacgg cgtgtagtcg ctcgtatggt ggaggtcggc
     1621 agcgcgcagc gtgcgccgcg ctgccggatc gaacaggacg agatcggcat cgcggccgac
     1681 ctcgatcgag cccttgcgcg agagcccgaa caggcgagca ggcgaggtcg acagcacgtc
     1741 gaccatgcgc tcgacgctca gccgtccctt cgcgacgccc tcggagtagg cgatcgcgag
     1801 cagcgtctcg atgcccggcg cgccgttgct gaccttgtcg aaggacacgc cgcgaacggc
     1861 ctcggccttt tcgacggcca tccgatcggg gacgtggtcg gtggcgatga ggtcgaggtc
     1921 gccattcgcg aggccggccc aaagtgcgtc ctggtccgat tgcggccgaa gcgggggcga
     1981 gatcacgcac tccgcgatgg aggcgagatc cggcgaggtg tatcgcgcgt cggtcagggc
     2041 gaggtagtgc gggcacgtct cggcgtgggc tcgaacgccg cgcgccttgg cgtcgcgcac
     2101 gtgacccaac gctgccgctg atgacaggtg gaccacatgg accggcacgt cggccgcccg
     2161 tgcgaacgcc attacccgat gcgtcgccac ggcctcggcc tcggtcgagc gggtggtggc
     2221 gtggaactgc ggcgcggtat cgccccgtgc cagtgcatcg gcgacggccg tatcgatcag
     2281 cactggatcc tcgcaatgca cctggagcat cccgcctcgg gcgcccagaa cgcgcatggc
     2341 ctcgtagatg cggcgctcgt cgagccggaa gtcgaagacc atgaacgcct tcgccgtggc
     2401 gacgccctca tcgaccatcg ccggcagctc ggcgaccggg tcgtccattc gaccgctgat
     2461 cgccgggttc agcgcgtagt cgaccgccga atctccctcc gtggccgccc gccactcgcg
     2521 aacacccgcc agcagcgacc gcccggcgac ccgcggcgag ccggtgcctg ggttgttgaa
     2581 ggcgaggaac gtcgtcgtcc cgccgaacgc cgccgccatc gagtcctgga agaaccgatc
     2641 gggctccgcg tcggttgcaa cccgcgtatg cgtatggaca tcgacggcgc ccgggagcac
     2701 gagcagcccc gtcgcatcga cgacctcgct cgccgacgcc gtgaggccgg acagatcggc
     2761 ctcgatcgcc tcgatcgagc cgctggagac ggccacatca gcaaggcgcg agcccgcggc
     2821 ggtgacgacc gtgccgcctc gaaccaggag gtcggtcatc ggcggcgcgc gagcattacg
     2881 tgcgcgggcg tcgtcgggcg ttcaccgcga ggatgatgac gacgatgatc gccacgagg
//
LOCUS       DATBSW010000285         4510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_908023, whole genome shotgun sequence.
ACCESSION   DATBSW010000285 DATBSW010000000
VERSION     DATBSW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908023"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VIF63_07965"
     CDS             <1..227
                     /locus_tag="VIF63_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239358.1"
                     /translation="KHARAYADLQGTIAAATRAWVADVVAGTFPGPAETVRMDDSVLD
                     EALGRHEDDRAAGGAPDRPSPSLIPLDRDL"
     gene            complement(232..822)
                     /locus_tag="VIF63_07970"
     CDS             complement(232..822)
                     /locus_tag="VIF63_07970"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY8239359.1"
                     /translation="MPTEYPDGRTRCAWPGTEPLNLEHHDVEWGVPSLDRRHLFELLT
                     LEGAQAGLSWLTILRKRAGYKRAFANFEPESVAAFGPDDVERLLRDEGIVRQRAKIEA
                     AISNARAILELERGGIDFVAHLWSFVGARPIENTFADGEMPAETAESRAMSKDLKKRG
                     FGFVGPTTCYSFMQSAGLVNDHTLACFRHAECAALA"
     gene            884..1723
                     /gene="panC"
                     /locus_tag="VIF63_07975"
     CDS             884..1723
                     /gene="panC"
                     /locus_tag="VIF63_07975"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8239360.1"
                     /translation="MAIQVVRTRAELRAALDAAPRPVGLVPTMGWLHAGHRALIARAR
                     GENATTVVSIFVNPRQFGDPADLERYPRNEARDVSISAEEGADLVFAPSVEEVYPPGF
                     DTTVSVGAVTLPLEGAARPGHFDGVATVVSILFNVVGADRAYFGQKDAQQLLVIRQMA
                     RDLAISTEVVTHPTVREPDGLAMSSRNVHLSPAERAAAPVVRQALLAAREAWLNGERS
                     AEVLRARMHAELAEEPLADLDYVSVADASTLAELETIDGPALLSMAVRFGSTRLIDNE
                     LLP"
     gene            1812..2498
                     /locus_tag="VIF63_07980"
     CDS             1812..2498
                     /locus_tag="VIF63_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY8239361.1"
                     /translation="MKLLLTSAGINNPSIRNALVDLLGKPIADSNALCIPTGSYGYPE
                     GNRPAGAWRFISGKSGQPMTELGWKSMGVLELTALPSIDRDRWVSWVREADVLLSSGG
                     DALYLAHWMRQSGLAELMPSLPEVVWAGFSAGSMVMAPRIGSDFVGWQPPTGGDEALG
                     VVDFSIFPHVDHPDLPENTMAAAEKWAAEIGGPAYAIDDATAIQVVDGTVEIVSEGHW
                     KLFNARGEAV"
     gene            complement(2495..3652)
                     /locus_tag="VIF63_07985"
     CDS             complement(2495..3652)
                     /locus_tag="VIF63_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8239362.1"
                     /translation="MRQLYLAATGQNRGKTTASLGLLDGFLRRGLYAGFMKPVGQRTV
                     IDDGEPADEDAVLMRTTFGLPEPYSVLSPVHIPRGFTKAYIAGEVVEDLPAKIAAAHA
                     RFANHDVLLIEGTGHAGVGAVIGLSNAVVAAQLGAPAIIVSEGGVGRPIDEIVLNASL
                     FAKHGVHVAGAIVNKVDVKAQPGIEKVLARGLAPYDIPLLGILPVHPILSNPTLEMII
                     EGTHGETLHPGPDLERVIDGVAIGAMEPGHMLERVGPGTLVIVPGDREDVILTLTTAH
                     LMGAPRTATTEEITFPTLQLDDGHAGAAIGLVLTGGYRPRKAVIEAIRRADLFATLVP
                     DDTYTVASEIHDLLVKTHASDVEKIELIKELVASNLDIDRILAVAVEARAS"
     gene            complement(3681..4286)
                     /locus_tag="VIF63_07990"
     CDS             complement(3681..4286)
                     /locus_tag="VIF63_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239363.1"
                     /translation="MLDHEEKFLFKEEILTLSNSLGEPGALDNVEDLLTDVITSPRFD
                     SEVFTLERSLQVMLGARPGTTLVGLLTVSPEVFDEVAEVRIPPEVHERLVAWRARFGL
                     AIDNALNFLNNPDGIQGHNFATQIAHVEERRVLQGRLTLVRYNNEPQFFVADAAVFLG
                     LVADVMERLGPYLETEAVDADTIGRLRDGVALIERKTTART"
BASE COUNT          641 a         1532 c         1612 g          725 t
ORIGIN      
        1 cgaagcacgc gcgcgcctat gcggacctcc agggcacgat cgccgcggcg acccgagcgt
       61 gggtcgcgga cgtcgtggcg ggcacgttcc cgggcccggc cgagacggtg cgcatggacg
      121 attcggtgct cgacgaggcg cttggccggc acgaggacga ccgcgccgcc ggcggcgccc
      181 ccgatcgccc ctccccgtcg ctgatcccgc tggaccgcga cctctaatcg ctcaggcgag
      241 cgcggcgcac tccgcgtggc ggaagcacgc cagcgtgtgg tcgttcacca gcccggccga
      301 ctgcatgaac gagtagcacg tcgtcgggcc gacgaagccg aagccgcgct tcttgaggtc
      361 cttcgacatc gctcgtgact cggcggtctc ggcgggcatc tcgccgtccg cgaaggtgtt
      421 ctcgatcggg cgcgcaccca cgaacgacca caggtgtgcg acgaagtcga tcccgccccg
      481 ttcgagctcg aggatcgcgc gggcgttgct gatcgctgcc tcgatcttcg cgcgctgccg
      541 gacgatcccc tcgtcgcgga gcagccgctc gacgtcatcg gggccgaagg cggcgacaga
      601 ctccggctcg aagttcgcga acgcccgttt gtagccggct cgcttgcgca ggatcgtgag
      661 ccaggacagg cccgcctggg cgccttcgag cgtcagcagc tcgaacaggt ggcgtcgatc
      721 gagcgacggc acgccccatt cgacatcgtg gtgctcgagg ttgagcggct cggtgcccgg
      781 ccaggcgcaa cgcgttcggc cgtcggggta ctcggtcggc atcgatcccg atgctagcgc
      841 cgtgcgccaa cccgtccgtc ggtcgatggc gtagcctccc ggcgtggcca tccaggtcgt
      901 tcgaacccgt gccgagctgc gcgccgcgct cgacgccgcg ccgcggcccg tcggcctcgt
      961 gccgacgatg ggctggctgc atgccggcca tcgggcgctg atcgcccgcg ctcgcggcga
     1021 gaacgcgacg accgtggttt cgatcttcgt gaatccgcgc cagttcggcg atccggccga
     1081 cctcgagcgc tacccgcgca atgaggcgcg cgacgtttcg atctccgccg aggagggtgc
     1141 cgacctcgtg ttcgcgccgt cggtcgagga ggtctacccg ccgggcttcg acacgacggt
     1201 gtccgtcggc gccgtcacgt tgccgctcga gggcgcggca cgccctggcc atttcgacgg
     1261 cgtcgcgacg gtcgtctcga tcctgttcaa cgtcgtcggc gccgatcggg cgtacttcgg
     1321 ccagaaggat gcccagcagc tgctcgtgat ccggcagatg gcgcgcgacc tggcgatctc
     1381 gaccgaggtc gtcacccacc ccaccgtccg cgagcccgac ggcctggcga tgtcatcgcg
     1441 gaatgtccac ctctccccgg ccgagcgcgc cgctgcgccc gtggtccggc aggcgctgct
     1501 cgcggcacgc gaggcctggc tcaatggcga gcgctctgcc gaggtgttgc gggcccgaat
     1561 gcacgccgag ctggcagagg agccgctcgc ggacctcgac tacgtctcgg tcgccgacgc
     1621 ctcgacgctc gccgagctgg aaacgatcga cgggccggcg ttgctgtcga tggccgtccg
     1681 attcggctcg acccgcctca tcgacaacga gctgctgcca tgacccacat cgtggcgtct
     1741 ggccgtccgg ccgtcggcgc cgcgctctac gccgcggtcg cgctcgagct cggtccggac
     1801 ctggaggtgt catgaagctg ctgctcacgt cggcgggcat caacaacccg agcatcagga
     1861 atgcactggt cgatctcctg ggcaagccga tcgccgattc caacgcgctg tgcatcccga
     1921 ccgggagcta cggatacccc gagggaaacc gtccggctgg ggcatggcgg ttcatcagcg
     1981 ggaagtccgg ccagcccatg accgagctgg gctggaagtc gatgggcgtg cttgagctca
     2041 ccgcgctgcc gagcatcgac agggatcgct gggtctcgtg ggtccgcgag gcggatgtcc
     2101 tgctgtcgag cggcggcgac gccctgtacc tggcgcactg gatgcggcag tctgggctgg
     2161 cggagctgat gccatcgctg cccgaggtgg tctgggcggg attcagcgcg gggagcatgg
     2221 tgatggcgcc gcggatcggg tcggacttcg tcggctggca gccgccgacc ggtggcgacg
     2281 aggcgctggg cgtcgtcgac ttctcgatct tcccgcacgt cgaccacccg gacctgcccg
     2341 agaacacgat ggccgccgcc gagaaatggg cagccgagat cggcggtccg gcgtacgcga
     2401 tcgacgacgc gaccgccatc caagtcgtcg acggcaccgt tgagatcgtc agcgaggggc
     2461 actggaagct gttcaacgcg cgcggggagg ccgtctagga cgcgcgtgcc tccacggcca
     2521 ccgccaggat gcggtcgatg tcgaggttcg aggccacgag ctccttgatc agctcgatct
     2581 tctcgacgtc cgacgcgtgc gtcttcacga ggaggtcgtg gatctccgag gcgacggtgt
     2641 acgtgtcatc ggggaccagg gtcgcgaaca ggtccgcgcg gcgaatggcc tcgatcaccg
     2701 ccttgcgcgg ccggtagccg cccgtcagga cgaggccgat cgcggcaccg gcgtggccgt
     2761 cgtcgagctg cagcgtcggg aacgtgatct cctcggtcgt cgcggtgcgc ggcgcgccca
     2821 tcaggtgcgc ggtcgtcagg gtgaggatca cgtcctcgcg gtcgccgggc acgatgacga
     2881 gcgtcccggg accgacgcgt tcgagcatgt ggcccggctc cattgcgccg atcgcgacgc
     2941 catcgatgac gcgctcgagg tcggggccgg ggtggagcgt ctcgccgtgc gtgccctcga
     3001 tgatcatctc gagcgtcggg ttcgacagga tcgggtgcac gggcaggatg ccgagcagcg
     3061 ggatgtcgta gggcgcgagg cctcgggcga ggaccttctc gatgccgggc tgggccttga
     3121 cgtcgacctt gttcacgatc gcgcccgcga cgtgcacgcc gtgcttcgcg aacaacgacg
     3181 cgttgagcac gatctcgtcg atcggcctgc cgacgccacc ctcgctgacg atgatcgccg
     3241 gtgcgccgag ctgggcggcg acgaccgcgt tcgacagccc gatgacggcg ccgacgccgg
     3301 cgtggcccgt gccctcgatc agcaggacgt cgtggttcgc gaaccgggcg tgcgccgcgg
     3361 cgatcttcgc gggcaggtcc tcgacgacct cgccggcgat gtatgccttc gtgaagccgc
     3421 gcgggatgtg gaccgggctg agcaccgagt acggctccgg gagcccgaac gtggtgcgca
     3481 tgaggacggc gtcttcgtcg gccggctcgc cgtcatcgat gacggttcgc tgcccgaccg
     3541 gcttcatgaa gcctgcgtac agcccgcgtc ggaggaaccc gtcgagcagg ccgagcgagg
     3601 ccgtggtctt gccgcggttc tggccggtgg ccgcgaggta gagctggcgc acgcggtcga
     3661 gcccttgctc cgagccgcgg ctatgttcga gcggtcgtct tgcgctcgat cagcgcgacg
     3721 ccgtcgcgca ggcgcccgat cgtgtcggcg tcgaccgcct cggtctcgag gtacggcccg
     3781 agccgttcca tcacgtcggc gacgagcccg aggaacacag cggcgtcggc gacgaagaac
     3841 tgcggctcgt tgttgtagcg aacgagggtg aggcggccct ggaggacgcg gcgctcctcg
     3901 acgtgggcga tctgggtcgc gaagttatgg ccctggatgc cgtcggggtt gttcaggaag
     3961 ttcagggcgt tgtcgatcgc cagcccgaac cgggcgcgcc acgcgaccag ccgctcgtgg
     4021 acttccggcg gaatgcgcac ctcggcgacc tcgtcgaaga cctcgggcga gaccgttagc
     4081 agcccgacca gcgtcgtgcc gggtcgcgcg ccgagcatca cctggaggct gcgctccagc
     4141 gtgaacacct cggagtcgaa ccgtggactc gtgatgacgt cggtcagaag gtcctcgacg
     4201 ttgtccagcg cccccggctc gccgagcgag ttcgagagcg tgaggatctc ctccttgaac
     4261 aggaatttct cttcgtggtc gagcatgcgc cgatcgtagc attcgcgatt ctgtggcccg
     4321 gcagcaaccc ggggttcgac gcggatccaa agtcgccgcc aggtcgtatc aacgcctacg
     4381 ggggtccacg gcacgctcgt acttggaggt ggagatgctc cgtaccacga cgttgcgcgt
     4441 cggcctggca ctgatcgtgc tcgccgtcgc ggcctacccg gtgatggccg gcaatggcat
     4501 gaagttcaag
//
LOCUS       DATBSW010000286         8134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_908071, whole genome shotgun sequence.
ACCESSION   DATBSW010000286 DATBSW010000000
VERSION     DATBSW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908071"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VIF63_07995"
     CDS             <1..1474
                     /locus_tag="VIF63_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239364.1"
                     /translation="AGTSFRVITDGSSQILATDLVKNRSYRLTGIVGQRASRKGALDG
                     YRLYLRDRGDISLGTSSSGGPGASGAPGASGAPGASGALGASGAPGASGAPAPITPVA
                     TVLALADGSHVTIEATVTAGVALLDSSGRRIVVQDATGAIEVFLPTGSTGPSLGARLR
                     ITGVTGHAWGAPRIAASAFDSLGSAAISPTTLSRAPAERDEWLLVRLSGSILKIERLG
                     DKWRAEIALANGTKAAVNGQAGAGIPSTAIIVGRRVTVVGIVKRPYPTASDRRFAVLP
                     RDGNDVSIGPAGNGSPSSSLGSGKDASKSAAGPNGTAALDITPDTDLAALLDRAGQRV
                     RVGGLIAHLADDGFDLDDGTALARIELRGDMAALVPHLREGEPVAITGTVEVVEGTAV
                     VVVDAEGTLVRVGSLGEALPVGGLAPAAGAESSAAAGANAIAADSTGLSTNLSSTSLL
                     AMLLIAALSVLVTVLRRRLVRRHLRAVLVDRLASLRSKAG"
     gene            1642..2481
                     /locus_tag="VIF63_08000"
     CDS             1642..2481
                     /locus_tag="VIF63_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEY8239365.1"
                     /translation="MPNADHRVALPVAERQYHIDLGPGELAEYILLPGDPDRTPRIAR
                     LLDDIELERRHREFASVTGTYRGQRVSVVSTGIGTDNVEIVMAEILAITERPTIIRVG
                     SCGGLQPEMALGDLVITTGAVRLETTTNWFVHDGYPAVADYEAVVALIEAAERLGNRY
                     HVGLTATAPGFFGAQGRPIPQLPIRYPDLAEDMARQRVMNFEMEASALLVLAGLARCR
                     AGVVCAVYAQRTTGDFVTGAAKDAAEAACVETGLEALGILADMDRRKREAATDRWRPS
                     LGI"
     gene            2545..2685
                     /locus_tag="VIF63_08005"
     CDS             2545..2685
                     /locus_tag="VIF63_08005"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEY8239366.1"
                     /translation="MAQAYCVKDKMKVEVQNPVQITMKNGKKALSGTCPKCGTKVFKI
                     GG"
     gene            2705..3925
                     /locus_tag="VIF63_08010"
     CDS             2705..3925
                     /locus_tag="VIF63_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8239367.1"
                     /translation="MDPRRATGGGLGFPGETARAVAVLDRDNRGIRRDANPRLLNSAP
                     FRTIETRGFALSAQHVIAVDLGGTRIRAAVIQPDGSRLARIDRPTPKDQGPAAVVAAC
                     IDAARDSRAAASTAAGGDIAGIGVSSPGPVNPRTGVVVETPNIGPMFKDIPLTAALGE
                     ALDLPAFLDRDTNVAALGERAFGAARDCDDFIYLTVSTGVGGSIVTGGEILHGPDGLA
                     GELGHIPVTLDGPRCGCGGIGHVEAYASGAGLAKLARAAVEDGRSPFLAKRATAVGGA
                     AKLSAVEVADGEDAGDPVCVELMTRARRAIAVGCVGYVHAFNPHRIVIGGSIAEAQGE
                     RLLGEVRETLRTEAFKAFSRHVTVVPAGLGGDVSLAGAHPLVMARLAARDRQSISNPT
                     RSDRPVAVPGGTHA"
     gene            3922..4947
                     /gene="gap"
                     /locus_tag="VIF63_08015"
     CDS             3922..4947
                     /gene="gap"
                     /locus_tag="VIF63_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8239368.1"
                     /translation="MTVRVGINGFGRIGRQSLKAIIERGTDVEVVAVNDLVDTSMNAL
                     LFKHDSTYGAYPGTVDHTDDALIIDGREIKVLKEKDPANLPWGDLGVDLVLESTGLFT
                     DAEKARAHITAGARKVIISAPAKGEDITIVLGVNDDKYDPAVHHVISNASCTTNCLAP
                     AAKVVHDLLGIERGLMNTIHSYTNDQRILDVAHKDPRRARAAGLNIIPTSTGAAKALA
                     LVIPDLKGKFDGFSLRVPTPTVSVVDFTADVARSTSVEELNEAFRAAAAGPMKGILGV
                     SDEPLVSSDFRGDSRSSIIDAASTMVLGGTMVKVIAWYDNEWGYSSRCADLISLVAAK
                     LPSGAAA"
     gene            4977..6164
                     /locus_tag="VIF63_08020"
                     /pseudo
     CDS             4977..6164
                     /locus_tag="VIF63_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
     gene            6266..7630
                     /gene="eno"
                     /locus_tag="VIF63_08025"
     CDS             6266..7630
                     /gene="eno"
                     /locus_tag="VIF63_08025"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY8239369.1"
                     /translation="MSAGTRIVRVNGREILDSRGNPTVEVDVTLADGSFGRAAVPSGA
                     STGINEAVELRDGDKARYGGKGVLRAVAGVNTEIGAAIAGLDATDQAVVDRTLIDLDG
                     TPTKQRLGANALLGASLATAHAAAAAAGQPLYRYLGGSGATTLPVPFFNILNGGKHAV
                     DSTDFQEFMVAPVGVATFAEAVRAGAEVFWALRSLLHDDGHSTGQGDEGGFAPSLPSN
                     EAAVEYILRAIEKAGYRPGEDVALALDPAVSSILVEGTGDHDTPGRYRLESEKRTLGT
                     DELIELWATWLDRYPIVSLEDGIGEADWAGWRALTERLGSRVQLVGDDLLVTNTAFIE
                     RGIAEHAANAVLIKLNQIGTLTETIGAIEMARAAGWGAMISHRSGETEDTTIADLVVA
                     LGTGQIKSGAPSRSERVAKYNRLLRIEEELGSEAHYAGRGALAKSGSAGSGIAGFAAP
                     APTR"
     gene            7627..>8134
                     /locus_tag="VIF63_08030"
     CDS             7627..>8134
                     /locus_tag="VIF63_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239370.1"
                     /translation="MSRFVDRGAVVAAYVGIGMAITMAIGLLLVIPIEPAYVLLSIPG
                     GMIIGYYANARSARSRGQWRRLIPNALLAGVVTGLVLASLALGTKALFFFGDSGFPDF
                     NRVENGVAVGPTCVAGGDCVYHRYLKAKPGDVSAAGITDSASFADVYWAQQWSTARLL
                     IGMTSSRPM"
BASE COUNT         1260 a         2904 c         2804 g         1166 t
ORIGIN      
        1 cgccggcacg agcttccgcg tcatcaccga cggatccagc cagattcttg ccaccgacct
       61 cgtcaagaac cgctcgtaca ggctcaccgg catcgtcggt cagcgggcgt cgcgcaaggg
      121 cgcgctcgat ggctaccggc tgtacctgcg cgaccgcggc gacatctcgc tcggcacgtc
      181 tagctcgggt ggtcccggtg cgagcggcgc accaggggcg agcggcgcac caggggcgag
      241 cggcgctctc ggtgcaagtg gtgctcccgg agcgagcggc gccccggccc ccatcacgcc
      301 ggtcgctacc gtcctcgcac tcgcggacgg ctcgcacgtg acgatcgagg ccaccgtgac
      361 cgcgggcgtg gcgctgctcg actcgagcgg ccggcggatc gtggtccagg acgccaccgg
      421 cgcgatcgag gtcttcctgc cgacgggctc caccgggccg agcctcggcg cccgacttcg
      481 gatcacgggc gtgaccggcc acgcgtgggg cgcgccgagg atcgcggcga gcgccttcga
      541 ctcgctcggg agcgcggcaa tctcaccgac cacgctgagc cgcgctccgg cagagcgcga
      601 cgagtggctg ctcgtgcgcc tctcgggttc gatcctcaag atcgagcggc tcggcgacaa
      661 gtggcgcgcc gagatcgccc tcgcgaacgg caccaaggca gcggtcaacg gccaggcggg
      721 cgccgggatc ccctcgactg cgatcatcgt cgggcgccgg gtcacggtgg tcggcatcgt
      781 gaagcggccg tacccgaccg cgagcgatcg ccggttcgcg gtcctgccac gtgacggcaa
      841 cgacgtctcg atcgggccgg ccggcaacgg cagcccatcg agctcgctcg gcagcggcaa
      901 ggacgcgtcg aagtcggcgg cggggcccaa cgggaccgcc gcgctcgaca tcacccccga
      961 caccgacctc gccgcgctgc tcgatcgagc cggccagcga gtccgggtcg gcggcctgat
     1021 cgcccacctc gccgacgacg gcttcgatct cgatgacgga accgcgctcg cccgcatcga
     1081 gctccgcggc gacatggcgg cgctcgttcc gcacctgcgg gaaggcgaac cggtggcgat
     1141 caccgggacg gtcgaggtcg tcgagggcac ggccgtggtc gtcgtcgacg ctgagggcac
     1201 gctcgtgcgc gtcggcagcc tcggcgaggc gctcccggtc ggcggtctcg cgcccgccgc
     1261 gggcgccgag tcgagcgccg ccgccggcgc gaacgcgatc gccgcggatt cgaccggcct
     1321 gagcacgaat ctgagctcga ccagcctcct cgccatgctg ttgatcgccg cgctgtcggt
     1381 gctcgtgacc gtgctccggc gccgtctggt ccggcgccat ctgcgggccg tgctcgtcga
     1441 tcggctggcg agcctgcgct cgaaggcggg ctgaactcgg acccgccgcg caacccgcca
     1501 ccccgccgag cgtggccccg agcgtggccc gaacgtggcc cgaacgggcg ttgagcgtga
     1561 tttcgcttga cgctcgttcg aacacgcgcc tacgcttgcc cgagttccgc acgtgcggga
     1621 aacgaggagg agaacgggat cttgccgaac gcggaccacc gcgtagcgct cccggtagcg
     1681 gagcggcagt accacatcga tctcggccct ggcgaattgg ccgagtacat cctcctcccc
     1741 ggcgaccccg atcgcacccc caggattgcc cggctgctcg acgacatcga gctcgagcgc
     1801 cggcaccgtg aattcgcttc ggtcaccggt acgtaccgtg gccagcgcgt atccgtcgtg
     1861 tcgacgggga tcggcaccga caacgtcgag atcgtgatgg ccgagattct cgccatcacg
     1921 gagcggccga cgatcatccg cgttggttcg tgcggcggcc tgcagccgga gatggccctc
     1981 ggtgatctcg tgatcaccac gggcgctgtt cgactggaga ccacgacgaa ctggttcgtc
     2041 cacgacggct atcccgccgt ggcggactac gaagcggtgg tcgccctcat cgaggccgcc
     2101 gaacggcttg ggaaccgcta tcacgtgggt ctgacggcaa cggcaccggg ctttttcggt
     2161 gcgcagggcc gcccgatccc gcagcttccc atccggtacc ctgacctcgc cgaggacatg
     2221 gcgcgccagc gcgtcatgaa tttcgagatg gaagcctcgg cgttgctcgt actcgcgggt
     2281 ctggcgcggt gtcgggccgg ggtggtctgt gcggtctacg cgcagcgcac cacgggagac
     2341 ttcgtgactg gggccgcaaa ggacgcggcg gaagcggcct gcgtggagac ggggctcgaa
     2401 gccctcggca tcctcgcgga catggaccgc cgcaagcgag aagcggcgac ggaccgctgg
     2461 cgaccgtcgc tcggaatttg agcgcagcgt gactccgggc accgagctcg gggcgcaacg
     2521 gagctacggg aggacctgaa acccatggca caggcctact gcgtcaagga caagatgaaa
     2581 gtcgaagtcc agaacccagt ccagatcacg atgaagaacg gcaagaaggc gctctctggc
     2641 acctgtccca agtgcgggac caaggtgttc aagatcggcg gctagcccct cctcatcgag
     2701 caccttggac ccccgccggg caaccggcgg gggtcttggt tttccgggcg aaacggcacg
     2761 tgcggtcgcg gtcctggatc gagacaatcg gggcatcagg agggatgcca accctcgact
     2821 gctaaactcg gccccgttcc gaacgatcga aaccagaggt ttcgccctgt ccgcccagca
     2881 cgtcatcgct gtcgacctcg ggggcacgcg gattcgcgcc gcggtgatcc agcccgatgg
     2941 cagccggctc gcccggatcg accggcccac gcccaaggac caggggccgg cggccgtcgt
     3001 cgccgcctgc atcgacgcgg cccgtgactc gcgggccgca gcttccacgg ccgccggggg
     3061 cgacatcgca gggatcggcg tgtcctcacc cggcccggtg aacccgcgga ccggcgtcgt
     3121 cgtcgagaca ccgaacatcg gcccgatgtt caaggacatc ccgctgaccg ccgccctcgg
     3181 cgaggcgctc gacctgcccg cattcctcga tcgagacacg aacgtcgccg ccctcggcga
     3241 gcgcgcattc ggagcggcgc gcgactgcga cgacttcatc tacctgaccg tttcgaccgg
     3301 cgtcggcggc tcgatcgtca ccggtggcga gatcctccac ggcccggacg gtcttgccgg
     3361 cgagctcggc catatcccgg tgacgctcga cgggccgcgc tgtggctgcg gcgggatcgg
     3421 gcacgtcgag gcgtacgcca gtggcgccgg gctcgcgaag ctcgcccggg cagcggtcga
     3481 ggacggccgc tcgccgttcc tcgcaaagcg cgcgaccgcg gtcggtggcg cggcgaagct
     3541 gtcggccgtc gaggtcgccg acggcgagga tgcgggcgat cccgtgtgcg tcgagctgat
     3601 gacccgcgcg cggcgcgcga tcgccgttgg ctgcgtcggg tacgtgcacg cgttcaaccc
     3661 gcaccgcatc gtgatcggcg gctcgatcgc cgaggcccag ggcgagcgcc tcctcggcga
     3721 ggtccgcgag accctgcgca cggaggcgtt caaggccttc agccggcacg tgaccgtcgt
     3781 tcccgccggc ctcggcggcg acgtgtccct cgccggcgcc catccgctcg tcatggcgcg
     3841 gctcgccgcg cgtgatcgcc aatccatttc caacccaacg cgatcggatc gtccggtcgc
     3901 ggtcccagga ggtacccacg catgactgtt cgcgtcggca tcaatggctt cggccgcatc
     3961 ggccgccagt cgctcaaggc catcatcgaa cgcggcaccg acgtcgaggt cgtcgccgtc
     4021 aacgacctcg tcgacacgag catgaacgcg ctcctgttca agcacgactc gacctacggc
     4081 gcctacccgg gaaccgtcga ccacaccgac gacgccctga tcatcgacgg ccgcgagatc
     4141 aaggtgctca aggagaagga cccggcaaac ctgccgtggg gcgacctcgg cgtcgacctc
     4201 gtgctggagt cgacaggtct gttcaccgac gccgagaagg cgcgagccca catcacggcg
     4261 ggcgcccgca aggtgatcat cagcgcgccc gcgaagggcg aggacatcac gatcgtcctg
     4321 ggcgtcaacg acgacaagta cgacccggcg gttcaccacg tgatcagcaa cgcgagctgc
     4381 acgacgaact gcctcgcacc ggccgcgaag gtcgtccacg acctcctggg catcgagcgc
     4441 gggttgatga acacgatcca cagctacacg aacgaccagc gcatcctcga cgtggcgcac
     4501 aaggacccgc gtcgtgcccg cgctgccggc ctgaacatca tcccgacatc gaccggcgcg
     4561 gccaaggcac tcgccctcgt catcccggat ctcaagggca agttcgacgg cttcagcctg
     4621 cgcgtcccga ccccgacggt cagcgtcgtc gacttcaccg ccgacgtcgc ccgctcgacg
     4681 agcgtcgagg agctgaacga agcgttccgc gccgccgccg cgggcccgat gaagggcatc
     4741 ctgggggtgt ccgacgagcc gctcgtctcc tccgacttcc ggggcgattc gcgctcgtcg
     4801 atcatcgacg ctgcgagcac gatggtgctg ggcggcacga tggtgaaggt catcgcctgg
     4861 tacgacaacg agtggggcta cagctcccgc tgcgccgacc tgatcagcct ggtcgccgcg
     4921 aagctgccgt ccggcgcggc cgcctgaccg cggcccggaa cgccgacgac cgatcgatgg
     4981 acaagctgac ggttcgcgac gccgatgtcg caggcaagcg cgtgttcgtg cgcgtcgact
     5041 tcaacgttcc gctcgaggac ggcaaggtca ccgacgattc gcgcatccgc gcgtcgatcc
     5101 cgaccatcca ttacctcctg acccacgggg caaaggtgat cctcgcgagc caccttggcc
     5161 ggcccgacgg caaggtccag gacggcctcc gcctccggcc ggtcgccgag cggctctcgc
     5221 agatcctcgg ccgcaacgtg ccggtcaccg gcgacgcctt gggcatcggc accgtcgatg
     5281 ccgtgaagcg gctccgcccc ggcgagatcc tgctcctcga gaacctccgg ttccatgccg
     5341 cggaggagaa gaacgacccc gcgttcgcgc agcagctcgc ggactacgcg gacgtgtacg
     5401 tcaacgacgc gttcgggacg gcccaccgcg ctcacgcgag cacggtcggc atcgcccgga
     5461 tcctcccggc gtacgcggga ttcctgatgg aacgcgagct cgcgatgctc tcgcggctca
     5521 tggagaaccc cgcgaagccc ttcgccgcga tcgtcggcgg ggcaaagatc tcgggcaaga
     5581 tcaaggtcct cgaccggctc atggacaagg tcgacgtgct catcctcggc ggcggcatgg
     5641 ccaacacgtt cctgctggcg cagggcaagg cggtcggcaa gagcctcgcc gagcacgaca
     5701 tggtcgatga ggcccggcgc gtccttgccg cggcggagaa ggccaaggtc gacgtgatcc
     5761 tgccggtcga cgtgatcgtc gccaaggagg tcacgcgcgg caccgagtac aagacgatcc
     5821 cggccgagaa gatccccgcg tcgtggcaca tcgtcgatgt cgggcggcag acgctcgacc
     5881 tgatggaggc ggcgctcgag acggcccaga ccgtgttctg gaacgggccg ctcggcgtgt
     5941 tcgagatccc gtcgttcgcc cacggcacgc gagcgatcgc gcgctacctg gcgacccgcg
     6001 ccgatgccgg cgcgacggtg gtggtcggtg gcggcgattc cgtcgccgcg atcacgcagc
     6061 agggcctggc cgagcggttc acccatatct ccaccggcgg cggcgcctcg ctggagttcc
     6121 tcgagggccg ggcgctgccc ggcgtcgatg tcctccagga ccggccgatc ctgacgcccg
     6181 cggagaaggg cctcgccacg aagcgggcga acgccgcgaa ggcggcgaag gcggccgagg
     6241 ccgagaagtc ggcgtcgagg gcgtcgtgag cgcgggaacg cgcatcgtcc gcgtcaacgg
     6301 acgcgagatt ctcgactccc gcggcaatcc gaccgtcgag gtcgacgtca ccctcgccga
     6361 cggctcgttc ggccgcgcgg cggttccctc cggggcctcg acgggcatca acgaggcagt
     6421 cgagctccgc gacggtgaca aggcgcgcta tggcggcaag ggcgtccttc gcgcggttgc
     6481 gggcgtcaac acggagatcg gcgccgccat tgccgggctc gacgcgacgg accaggcggt
     6541 cgttgatcga accctgatcg atctcgacgg cacgcccaca aagcaacggc ttggcgcgaa
     6601 tgcgctcctc ggcgcctcgc tcgcgacggc gcatgcggcc gcggcggccg ccggccagcc
     6661 cctctaccgc tacctcggtg gatcgggtgc gaccacgctg ccggtgccgt tcttcaacat
     6721 cctcaacggt ggcaagcacg ccgtcgactc gaccgacttc caggagttca tggtcgcgcc
     6781 ggtcggcgtc gcgacctttg ccgaggcggt tcgtgccggt gctgaggtct tctgggcgct
     6841 gcggtcgctc ctgcatgacg acggccattc cacagggcaa ggggacgagg gcggttttgc
     6901 cccgagcctg ccgtcgaacg aggcggccgt cgaatacatc ctgcgggcga tcgagaaggc
     6961 gggctaccgg cccggtgagg atgtcgcgct cgcgctcgac cccgccgtca gctcgatcct
     7021 cgtcgagggc accggcgacc acgacacgcc cggtcgctac cggctcgaga gcgagaagcg
     7081 gacactcggg accgacgagc tgatcgagtt gtgggcaacc tggctcgacc gctacccgat
     7141 cgtgtcgctc gaggacggca tcggcgaggc cgactgggcc ggctggcgag cgctgaccga
     7201 gcgcctcggc tcgcgcgtcc agctcgtcgg cgacgacctg ctcgtcacca acaccgcctt
     7261 catcgagcgg ggcatcgcgg aacacgccgc gaacgcggtg ctgatcaagc tcaaccagat
     7321 cggcacgctg accgaaacca tcggcgcgat cgagatggcc cgcgccgcgg gctggggggc
     7381 gatgatcagc catcggtcgg gcgagaccga ggacacgacc atcgccgacc tcgtcgtcgc
     7441 gctcggcacg ggccagatca agtccggcgc gccgtcgcgc tccgagcggg tcgccaagta
     7501 caaccggttg ctccggatcg aggaggagct cggcagcgag gctcactacg cggggcgtgg
     7561 ggcactcgcg aagtcgggtt ccgcgggctc ggggatcgcc ggattcgccg cgcctgcacc
     7621 cacgcggtga gccggttcgt cgaccgcggt gcggtcgtgg cggcctacgt cgggatcggc
     7681 atggccatca cgatggccat cggcctgctg ctcgtgatcc cgatcgagcc ggcctacgtc
     7741 ctgctgtcga tcccgggcgg catgatcatc ggctactacg ccaacgcccg gtcggcgcgg
     7801 tcgcgcggcc agtggcggcg actcattccg aacgcgctgc tcgccggcgt ggtgacgggg
     7861 ctcgtcctcg cctcgctcgc gctcggcacg aaggcgctgt tcttcttcgg cgacagcggc
     7921 ttccccgact tcaatcgagt cgaaaacggc gtcgccgtcg gcccgacgtg cgtcgcgggt
     7981 ggcgattgcg tctaccaccg ctacctcaag gccaagcccg gtgacgtctc cgctgccggc
     8041 atcaccgact ccgcttcctt cgccgacgtg tactgggcgc agcagtggtc gaccgcgcgc
     8101 ctgctcatcg ggatgacgag cagcaggccg atgg
//
LOCUS       DATBSW010000287         3635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_379955, whole genome shotgun sequence.
ACCESSION   DATBSW010000287 DATBSW010000000
VERSION     DATBSW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379955"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VIF63_08035"
     CDS             complement(<1..1196)
                     /locus_tag="VIF63_08035"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8239371.1"
                     /translation="MTPSKGRAAHRRVAGLIALAAWLATFAVGQTGAIALTLLALPDS
                     YSVHHDRTLNVAAPGVLANDVGIGGDPQPHVDSDPTHGVVVVDPDGSFTYTPDNGYAG
                     TDVFQYHVHNGLLSSNSVNVTITVTNAAPVAKDESYTAQTGVKISVPAPGVLANDTDA
                     DGDGLTVEFIDTGGNGSMDGHPNGSFTFKSGGSFEGDRTFDYRVSDGITWSNVATLTI
                     RVSSSPTPTPAPTATAAPTPTPAPTRTPGPTPTPKPLPTLPLPTLPLPTLPPLPTLPP
                     LPTLAPPLPTATPSPRPTPAVSTAPPSTSPSPSTEASTPPASAVPGSTAGPDASSGPG
                     STAGAPSGPGADFGIGGTSQGGAGGIPIDGIDGVMAGALGALPGGFLAWSYPAIIIAG
                     PGILLL"
     gene            complement(1193..3052)
                     /locus_tag="VIF63_08040"
     CDS             complement(1193..3052)
                     /locus_tag="VIF63_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8239372.1"
                     /translation="MKAGTTGATGFFGASPRAPRQVLLLIVFGVLLAVVGVTATAQAV
                     MVTVYSSKSTLNSILESDLATIRGFVYQGLAPSLLEPNGLSPQEQSALEGLIGTIESK
                     GGILRVELRLPDGRVLAASDTASAGSLVPSSVDFAGAVADGNAQVALVEAGAAEAAPG
                     TPFETQQLLREYLPLRLGEQVVLVVGVWRDAVPILGRLDDLRRDVVLVTISAALIAAL
                     TLYFVFRAAQARLGRQARALVEASRRDPLTGLLNHGAMVGLLATEVERARADRSSLGI
                     ALIDLDGFRLLNDNHGHRAGDEALVSVAELLNATVADDRLLVGRYGPDEFLLVSTTHD
                     GDALESVVEDIRTRLAGVVLQFETTERMPLTVSAGLCTYPIHGASVTTLLSTAVRTLE
                     DAKSGGGNSVRVAQVEEEPTAGGTSFDVLQGLVIAVDKKDRYTKRHSEDVSRYAVFIA
                     ELLGLDEDTIRALRIAGLLHDVGKIGIPDAILRKPGRLTAVEEQVIHQHVALGDSIVR
                     DLPDIELIRAGVRHHHERWDGTGYLERLEGEAIPLVARILAVGDAFSAMTTTRPYRKA
                     LGVEEAMRRLGDAAGTQLDAALVTTFLVGIETAADPPLPDQGPIARRASLHVA"
     gene            3162..>3635
                     /locus_tag="VIF63_08045"
     CDS             3162..>3635
                     /locus_tag="VIF63_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8239373.1"
                     /translation="MPHERPEPVIAVVGSLNIDLVAYTRRVPGPGETVIGDRFVMGFG
                     GKGANQAVMAARLGARVSFVGCVGDDALGAETVANLTANGVDTTGIRVVAETATGVAP
                     IWVDERGANRILIAPGANNALDATHVRSALDALVRPPDVVVAQLETPQSATAAAFA"
BASE COUNT          536 a         1215 c         1347 g          537 t
ORIGIN      
        1 atcagcagga ggatgcccgg gccggcgatg atgattgccg ggtaggacca cgccaggaag
       61 ccgccgggca gggccccgag ggcgccggcc atcacgccgt cgatgccatc gatcgggatg
      121 ccgcccgccc caccttgcga ggttccaccg atgccaaagt ctgcgcccgg accggatggc
      181 gccccggcgg tggatcccgg gccgctcgag gcatctgggc ctgctgtcga gccaggcact
      241 gcggaggccg gcggggtcga tgcctcagtc gaaggtgatg ggctcgtgct cggcggcgcc
      301 gtggagacgg cgggcgtcgg gcgcggcgat ggcgttgcgg tgggcagcgg cggggccagc
      361 gtcgggagcg gcggcagcgt gggcagcggc ggcaacgtgg gcagtggcaa ggttggaagc
      421 gggagggtgg gcagcggctt gggggtcggt gtgggtccgg gagtgcgcgt cggcgcaggc
      481 gtcggggtcg gcgcggctgt cgcggtcggc gcaggcgtcg gggtcggcga gctgctgacc
      541 cggatcgtca aggtggccac gttcgaccac gtgattccat ccgagacgcg atagtcgaag
      601 gtccggtcgc cctcgaacga acccccggac ttgaaggtga agctgccgtt cgggtgcccg
      661 tccatcgagc cgttgccgcc ggtatcgatg aactcgacgg tgagtccgtc cccatccgcg
      721 tcggtgtcgt tggccaggac gcccggcgcc ggcaccgaga tcttgacgcc ggtctgtgcc
      781 gtgtatgact cgtccttggc gacaggagcg gcgttcgtga cggtgatcgt gacgttgacc
      841 gaattgctgc tcagcagccc gttgtggacg tggtactgga agacatccgt gccggcgtag
      901 ccgttgtcgg gcgtgtaggt aaagctgccg tcggggtcga ccaccaccac cccatgggtc
      961 gggtccgaat cgacgtgcgg ttgtgggtcc ccgccgatac ccacgtcgtt cgcgaggacg
     1021 cccggggcag ccacgttcaa ggtgcgatcg tggtggaccg agtacgagtc cggcagcgca
     1081 aggagcgtca gcgcaatcgc cccggtctgg ccgacggcga aggtcgcaag ccatgcggcg
     1141 agggcgatga ggccggcaac gcgccggtgc gcggcgcggc ccttcgacgg ggtcatgcga
     1201 cgtgcaggct cgcccgacgc gcgatcgggc cctggtccgg cagcggcgga tcggcggccg
     1261 tctcgatccc gacgaggaac gtcgtcacga gtgcggcatc gagctgcgtt ccggccgcgt
     1321 cgcccagccg ccgcatcgcc tcttccacgc cgagcgcctt ccggtaggga cgcgtggtcg
     1381 tcattgccga gaaggcgtcg ccgaccgcga ggatcctcgc cacgagaggg atggcctcgc
     1441 cctcgaggcg ttcgaggtag ccggtgccgt cccatcgttc gtggtggtgc cggacgcccg
     1501 cccggatcag ctcgatgtcc ggcaggtcgc gcacgatgct gtcgccgagg gcgacgtgct
     1561 gatggatgac ctgctcctcg accgccgtga ggcggcccgg cttgcgcagg atcgcgtcgg
     1621 ggatcccgat cttgcccacg tcatggagca ggccggcgat gcggagtgcg cgaatcgtgt
     1681 cctcgtcgag gccgaggagc tcggcgatga acacggcgta gcgcgacacg tcctcggagt
     1741 gccgcttcgt atagcgatcc ttcttgtcga ccgcgatcac gaggccctgg aggacatcga
     1801 agctcgtccc gccagccgtg ggttcctcct cgacctgggc cacccggacg gagttgccgc
     1861 cgcccgactt ggcgtcctcg agggtgcgca ccgccgtcga cagcagcgtg gtcacggacg
     1921 cgccgtggat cggatacgtg cacaagcccg cgctgacggt cagcggcatc cgctccgtcg
     1981 tttcgaactg gagcacgacg cccgcgagac gcgtgcggat gtcctcgacg accgattcca
     2041 gcgcgtcgcc gtcgtgtgtc gtcgatacga gcaggaactc gtccggaccg tagcggccca
     2101 cgagcagccg atcgtcggcg acggtggcgt tcagcagctc ggccaccgaa acgagggctt
     2161 cgtcgccggc gcggtggccg tggttgtcgt tcaggaggcg gaagccgtcg aggtcgatga
     2221 gcgcgatccc gagtgaggaa cgatcggccc gggcgcgctc cacctcggtg gcgagcaggc
     2281 cgaccatcgc gccgtggttc aggaggccgg tcagtggatc ccgacggctc gcctcgacga
     2341 gggcccgggc ctggcggccc agccgggcct gcgccgcccg gaacacgaag tacagcgtga
     2401 gggccgcgat cagggccgcc gagatcgtga cgaggacgac gtcgcgtcgg aggtcgtcga
     2461 gcctgcccag gatggggacc gcatcacgcc agacgccgac gacgagcacg acctgctcgc
     2521 cgagcctgag gggcaggtac tcccgcaaca gctgctgtgt ttcgaagggc gtaccgggcg
     2581 ccgcttcggc ggcgccggcc tcgaccagtg cgacctgggc attgccatcg gcgacggcgc
     2641 ccgcgaaatc cacgctcgac gggaccaggc tgcccgcgct tgcggtgtca ctggcggcga
     2701 ggacgcggcc gtcggggagc cgcagctcga cgcgcaggat ccctcccttg ctttcgatcg
     2761 tcccgatgag cccttcgagc gccgactgct cctgcggcga cagtccgttc ggctcgagga
     2821 ggctcggggc gaggccctgg tacacgaagc cccggatcgt tgccaggtca ctctcgagaa
     2881 tcgagttgag cgtcgatttg gatgagtaca ccgtgaccat gacggcctgc gccgtcgcgg
     2941 tcacgccgac cacggcgagg agcacgccga acacgatgag cagcaggacc tgccgcggcg
     3001 cgcgcggcga tgccccgaag aatccggtcg ccccggtcgt cccggctttc acatcggcga
     3061 aggtattgag tctttcagcg ttccaacagg gaacgaaggt cccatgtgcc ggggcgcacc
     3121 ggccggggcc ccggtagcgg acaatcgggg cggaggtacc gatgccgcac gaacgacccg
     3181 aaccggtcat cgccgtcgtc ggcagcctga acatcgatct cgttgcctac acgcgccggg
     3241 tgccgggtcc gggcgagacg gtcatcggcg atcgattcgt catgggcttc ggcggcaagg
     3301 gcgcgaacca ggccgtcatg gccgcgcgcc tcggtgcccg ggtgtcgttc gtcggatgcg
     3361 tgggcgacga cgcgctgggc gcggagaccg tcgccaacct caccgccaac ggcgtcgaca
     3421 ccaccggcat ccgggtggtc gccgagacgg ccaccggcgt ggcgccgatc tgggtcgatg
     3481 aacgtggcgc caaccgcatc ctcatcgccc caggcgccaa caacgccctc gacgccacgc
     3541 acgtccgctc ggcactggac gccctcgtcc ggccgccgga cgtggtggtc gcccagctcg
     3601 agacgcccca gtccgcaacg gcggcggcgt tcgca
//
LOCUS       DATBSW010000288         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_238628, whole genome shotgun sequence.
ACCESSION   DATBSW010000288 DATBSW010000000
VERSION     DATBSW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238628"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..675)
                     /locus_tag="VIF63_08050"
     CDS             complement(103..675)
                     /locus_tag="VIF63_08050"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY8239374.1"
                     /translation="MVIADVSGKGISAALLMAFVRPVMRSALDRTADPIRALELTNHI
                     LVDERRTGLFVTVLAGVLDLDTGVFNFANAGHEMPLIASLAGEPRWVPGGGPLLGVFG
                     RLDLAPLSVEIRAGDRLVLYTDGITDAKGRTGGRFGDERLRTAIHDSCDRHADDTCGS
                     VIDRVLAFQGDAEPADDLALLVLRRMPAAS"
     gene            complement(868..1284)
                     /locus_tag="VIF63_08055"
     CDS             complement(868..1284)
                     /locus_tag="VIF63_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8239375.1"
                     /translation="MTIDVEHLGGSPSVTVVALDGELDASNYERVIEIVRDAYARGAR
                     ALVLDLAHLSFMASSGLFALHSAVRIMRGETPPDPEMGWGALHEVASDHDSAASNVRL
                     AGAQDAISRVLERTGMASLFGIDANRADALAALQGA"
     gene            complement(1294..>1761)
                     /locus_tag="VIF63_08060"
     CDS             complement(1294..>1761)
                     /locus_tag="VIF63_08060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8239376.1"
                     /translation="WPDPVMSADDPTPMHLRVESDTDRLADVRAFVRDAVASFGGSKA
                     VADDLVQAVDEATCNVMLHGYGHAPGSIDIEAAVRDGRIEIRLLDRAPAFDPTAAAAR
                     DTKLAPVPKRPGGMGVGIHLLRTMTDEVRHHVRPDGGNELTLVRSIDDAAREG"
BASE COUNT          275 a          614 c          620 g          252 t
ORIGIN      
        1 aaggaccgcc tgctcttcga cttcaacgag cgctggcgca gcgtgatggg gcgaccgccc
       61 gccggctgag ggccctgggc gcattcgccg cccgcccgcg atctacgacg ccgcgggcat
      121 ccttcggagc acgagcaggg cgagatcgtc ggccggctcc gcgtcgccct ggaacgccag
      181 gacgcgatcg atcacagagc cacaggtgtc gtcggcgtgg cggtcgcacg agtcgtggat
      241 cgccgtgcgc agccgctcgt cgccgaaacg cccgccggtg cggcccttgg cgtcggtgat
      301 gccgtcggtg tagaggacca gtcggtcgcc cgcccggatc tcgaccgaca atggcgccag
      361 gtcgagccgg ccgaagacac cgagcagtgg cccgccgccc ggcacccagc gtggctcgcc
      421 ggcgagggag gcgatgagcg gcatctcgtg gccggcattg gcaaagttga agacaccggt
      481 gtcgaggtca agcactcctg cgaggactgt gacgaacagc cccgtccgtc gttcgtcgac
      541 gaggatgtgg ttcgtcagct cgagggccct gatcggatcc gccgtgcggt cgagcgccga
      601 gcgcatgacg ggccgcacga acgccatcag gagcgcagcc gagatgccct tgcccgagac
      661 gtcggcgatg accacgccga acttgcgagg ctggtcgatg atcgggaaga cgtcgaagaa
      721 gtcgccaccg atggagcgcg cgggccggta gtcgctggcg atctcccagc cgtcggcttc
      781 gacgcccgac agcaggacga gggtgcgctg gattcgctgg gcgacctgga gctcgcgggc
      841 gtagtactcg gcgtcgctgg ccgtcggtca ggctccctgg agcgcggcga gggcgtccgc
      901 ccggttcgcg tcgatcccga agagcgacgc catccccgtc cgctcgagca cgcggctgat
      961 cgcgtcctgg gcgccggcga gccgcacgtt cgaggcggcc gaatcgtggt ccgaggcgac
     1021 ttcgtgcagc gcgccccagc ccatctcggg atcgggcggc gtctcgccgc gcatgatccg
     1081 gaccgcggag tgcagcgcga acagccccga gctggccatg aacgacaggt gggcgaggtc
     1141 gaggacgagc gcgcgcgccc cgcgggcata cgcgtcacga acgatctcga tcacccgctc
     1201 gtagttcgac gcgtcgagct caccgtcgag cgcgacgacc gtcacggacg gcgacccgcc
     1261 aaggtgttcg acatcgatcg tcatcgccac cggtcaaccc tccctggccg cgtcgtcgat
     1321 cgagcgcacg agggtgagct cgttgcctcc atcgggacgg acatgatgcc gcacctcatc
     1381 ggtcatcgtg cggagcagat ggatgccgac gcccatgcca ccgggccgct tcggcaccgg
     1441 cgcgagcttc gtgtccctgg cggctgccgc cgtcggatcg aacgccggcg cccgatcgag
     1501 cagtcggatc tcgatcctcc cgtcacggac cgcggcctcg atgtcgatcg atcccggggc
     1561 gtggccgtag ccgtggagca tcacgttgca cgtcgcctcg tcgactgcct ggaccaggtc
     1621 atcggcgacc gccttcgagc caccgaagct ggcaacagca tctcgaacga aggcgcggac
     1681 gtcggcgagc cgatccgtgt ccgactcgac ccgcaggtgc atcggcgtcg gatcgtccgc
     1741 gctcatgacc ggatcaggcc a
//
LOCUS       DATBSW010000289         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1225926, whole genome shotgun sequence.
ACCESSION   DATBSW010000289 DATBSW010000000
VERSION     DATBSW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225926"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VIF63_08065"
     CDS             complement(<1..559)
                     /locus_tag="VIF63_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239377.1"
                     /translation="MDPIGQRMRNRVLPALLTATGFALLASGLMSYTTAVEAPPSNRP
                     LAVYEPLPTPIGNITLPGSTSGPVDPGFPEDRVATRIVIRRLNIDLPVMLQTDNYGFY
                     PLCDVALYIPQMGQPGEGRATYLYAHAREGMFLPLLRRSERADDGESMIGMAVEVYTS
                     DNWRFLYTITEVRRHTLNLNDAMNTT"
     gene            complement(666..>1622)
                     /locus_tag="VIF63_08070"
     CDS             complement(666..>1622)
                     /locus_tag="VIF63_08070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY8239378.1"
                     /translation="FAWGATGTTSIAGVRAALEGVAAGSAALSPALVLGLGFMTTGVA
                     FKIAAVPFHYWTPDAYQGSPTPVTGYLSVGPKVGAFALILRLFVEALGPLRLDWLQVV
                     VILAVLTMTLGNLVALTQNNVKRMLAYSSIAHTGYMLVGLAAFAAGRIEGLEGLLYYG
                     AAYSVMNLGAFAVVAAIQRRAGVTSGLDTFAGLGRREPWLAILMTLFLLSLTGIPPTT
                     GFFAKAYVILAAVQASDAGMTSLGILAVVAVLNAAAAAFYYLRVVVYMFMREPRTEEA
                     AMPHGRLMWGGLVVASVLTVVLGLIPGPFLDIVARAALAIGG"
BASE COUNT          273 a          546 c          588 g          215 t
ORIGIN      
        1 tcgtggtgtt catcgcgtcg ttgaggttga gcgtgtggcg gcggacctcg gtgatcgtgt
       61 acaggaaccg ccagttgtcg ctcgtgtaga cctcgaccgc catgccgatc atcgattcgc
      121 cgtcgtccgc acgctccgac cgcctgagca acggcaggaa catgccttca cgggcgtggg
      181 cgtagaggta cgtcgcccgg ccctcgccgg gctggcccat ctgggggatg tagagcgcga
      241 cgtcgcacag cggatagaac ccgtagttgt cggtctggag catgaccggc aggtcgatgt
      301 tgagccggcg gatcacgatc cgggtcgcga cgcgatcctc cgggaagccc ggatcgacgg
      361 gtccgctggt gctgcccggg agcgtgatgt tgccgatcgg cgtcggcagc ggctcgtaga
      421 cggcgagcgg gcggttcgat ggtggcgcct cgaccgcggt cgtgtacgac atcaggccgc
      481 tggcgagcag cgcgaacccg gtcgcggtca ggagggcagg caagacccgg ttgcgcatgc
      541 gctgcccgat cggatccatg gccgcgaggc taccagcaga tgccggtgca cagggtcgac
      601 gcgacccttg gcgacccgtg gcgacccttg gcgacccgtg gcgacccgtg gcgcgacccg
      661 ccgagctacc cgccgatggc cagcgcggct cgcgcgacga tgtccaggaa cggccccgga
      721 atgagcccga gcaccacggt cagcacgctc gcgacgacca gcccgcccca catcagccgg
      781 ccgtggggca tcgccgcctc ctcggttcgc ggctcgcgca tgaacatgta gacgacgacg
      841 cggaggtagt agaacgcggc ggcggcggca ttgagcaccg cgacgacggc gaggatcccg
      901 agcgacgtca tgcccgcgtc gctcgcctgc acggccgcga ggatcacgta cgccttcgcg
      961 aagaagccgg tggtcggcgg gatgccggtc agcgagagca ggaacagggt catgagaatc
     1021 gcgagccacg gctcacgccg gccgaggccg gcgaacgtgt cgaggccgga cgtgacgccg
     1081 gcgcggcgct ggatcgccgc cacgacggcg aacgcaccga ggttcatcac cgagtacgcc
     1141 gcgccgtagt agagcaggcc ctcgaggcct tcgatcctcc cagccgcgaa cgccgccagg
     1201 ccaacgagca tgtagccggt atgcgcgatc gacgagtacg cgagcatccg cttgacgttg
     1261 ttctgggtca gcgcgacgag gttgccgagg gtcatcgtca ggacggcaag gatgacgacg
     1321 acctggagcc agtcgaggcg gagcggcccc agcgcctcga cgaacagccg caggatcagc
     1381 gcgaacgcac cgaccttcgg tccgaccgag aggtacccgg tcaccggcgt cggcgagccc
     1441 tggtatgcgt cgggagtcca gtagtggaac gggaccgccg cgatcttgaa cgcgacgccg
     1501 gtcgtcatga acccgaggcc gagcacgagc gcgggcgaca gggcggccga gcccgccgcg
     1561 acaccctcca gcgccgcgcg gaccccggcg atgctcgtcg tgccggtcgc gccccacgcg
     1621 aa
//
LOCUS       DATBSW010000290         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_873776, whole genome shotgun sequence.
ACCESSION   DATBSW010000290 DATBSW010000000
VERSION     DATBSW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873776"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..592
                     /locus_tag="VIF63_08075"
     CDS             89..592
                     /locus_tag="VIF63_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239379.1"
                     /translation="MFTRIFGVARPILVALVALFLVAGAVFAGNAILASSPATVSSPG
                     ESPEATESAEPSESPEAAETAEPSESPEATVDNEDVNDNDDDASPGATDDHGANSGHD
                     GADDATDDHGGASGDDDADDATDDHGGDSGPGDNAGPGGDDDSADDNSGPGRETSEPG
                     DDNGGHG"
     gene            656..1303
                     /locus_tag="VIF63_08080"
     CDS             656..1303
                     /locus_tag="VIF63_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8239380.1"
                     /translation="MASTPVDRPSGASPWGPPIDSDRHARPLDDADIVRSILDGDRDA
                     FRVLVDRESTSVVRACVRVLGDLHEAEDIAQEAFVTAYRSLATWRGDGPFGAWLTRIA
                     VRLAVRQLGRRREVSWIGQLDVAGTADRVAALPASPRSQPESVALRREDATATRRSVA
                     NLGEPYREVVALRFFGERSLEEIAQITGRPLGTVKTHLRRGLLKLRDDIGGTGRP"
     gene            1300..2073
                     /locus_tag="VIF63_08085"
     CDS             1300..2073
                     /locus_tag="VIF63_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239381.1"
                     /translation="MTTRRPFDPEDLGAASPDDVAAAMDMGRRLDAATTDQGSIAPSA
                     GFGDRVMAAIADEPAPSNVGFLMPLRRRGVLGGFAVSVRQAWQSLGGAGRPTFVRASA
                     LAYVLAVAIAGSAVAGALTVGVAGALGILGPTATESPPPTSPAPDITPAPTVAPTVAP
                     VETPPSSLPPPSPSESAEPSDDHGGGSGPEPSDDHGGGPGPEGTDDHGGNSGPGGGGS
                     DDGSGSDDSGSGSGSDSSTPKPSDTPRPTGTPKPTETPH"
     gene            complement(2083..>2812)
                     /locus_tag="VIF63_08090"
     CDS             complement(2083..>2812)
                     /locus_tag="VIF63_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239382.1"
                     /translation="GEWGDAPPTFDRSVDAWGTGAPAFADEGEGGGEQYVELIGTELW
                     VAGIVNLGRFRRVSDFVNIVQGYMVIRDVVVLTRVGGATRLTLPELRVLPDDISVVGQ
                     LASDGSGSTGAAGDLIEKTSQRLVVLTRSHVIDGDVFILGDGTIMSFVDATDPKFIPM
                     TNVRVRWVSDRKLAARYPFALVQRSQILGVATEGIKLGGAESTMRRASMLKAQARPGP
                     AQGDGLDIDTVPAEAGSETAADAG"
BASE COUNT          427 a         1012 c          981 g          392 t
ORIGIN      
        1 ggtgctgaaa ccttttccgc gttggtcgcc tcaggttggt agcggacggt cgcgatgacc
       61 gccacgagca ccaaggagcc cagcccacat gttcacccgc atcttcggcg tcgcgcgacc
      121 catcctcgtc gcgctcgtcg ccctgttcct ggtcgccgga gccgtcttcg ccggcaacgc
      181 gatcctcgcc tcgagcccag cgacggtgtc gtccccgggc gagtcgcccg aggcgaccga
      241 atctgccgag ccgagcgagt cgcccgaggc ggccgagacc gccgagccga gcgagtcgcc
      301 cgaggcgacc gttgacaacg aagacgtcaa cgacaacgac gacgacgctt cgcctggcgc
      361 gacggacgac catggcgcga actccggcca tgacggcgcg gacgacgcga ccgacgacca
      421 tggcggtgcg tcgggtgacg acgacgcgga tgacgcgacc gacgaccacg gtggcgattc
      481 gggccccggc gacaacgccg gccccggtgg cgacgacgac agcgccgatg acaactccgg
      541 cccagggcgc gagacgtccg agccgggcga tgacaacggc ggccacggct gagccgttcg
      601 gccctgccgg atcgtgacct ctgcccgacg atgggaagcc ccgaagcgcc ggatcatggc
      661 gtcgacaccg gtcgatcgtc cgtccggcgc ttcgccatgg gggcctccca tcgactcgga
      721 ccgccacgcc aggccgctcg atgatgccga catcgtccgt tcgatcctcg acggcgaccg
      781 ggacgcgttc cgggtcctcg tcgaccgcga gtcgacgtcg gtcgtgcggg cctgcgttcg
      841 agtcctcggc gacctccacg aggccgagga catcgcccag gaggcgttcg tgactgcgta
      901 ccgctccctg gcgacctggc gcggcgacgg accattcggg gcctggctga cgcgcatcgc
      961 cgtgcggctt gccgttcgcc agctcgggcg ccggcgcgag gtttcctgga tcgggcagct
     1021 ggatgtcgcg gggacggccg atcgtgtcgc ggcgctcccg gcaagcccgc ggtcccagcc
     1081 cgagtccgtg gcccttcgga gagaggacgc cacggcgact cgccgatccg tggccaacct
     1141 cggcgagccc taccgtgagg tcgtggcgct gcggttcttc ggcgagcgat cgctcgagga
     1201 gatcgcccag atcaccggcc ggcccctcgg taccgtgaag acgcacctgc ggcgaggact
     1261 gctcaagctg cgcgacgaca tcggcgggac gggacggcca tgacgacccg ccgaccgttc
     1321 gatcccgagg acctgggcgc ggcctcgccg gacgacgtcg ccgccgcgat ggacatgggc
     1381 cgccggctcg acgccgcgac gacagatcag gggtcgatcg cgccgagcgc cggctttggc
     1441 gatcgggtca tggccgcgat cgccgatgag ccggccccct cgaacgtcgg cttcctgatg
     1501 ccgcttcgac gtcgcggcgt cctcggcggc ttcgcggtga gcgtgcgcca agcctggcag
     1561 tcgctcggcg gcgccggtcg acccacgttc gttcgagcgt cggcccttgc atatgtgctc
     1621 gctgtcgcga tcgcgggcag cgccgtggca ggcgcgctca cggttggcgt tgcgggcgcg
     1681 ctgggcatcc tcggaccgac tgcgacggaa tcgccgccgc caacgtcgcc tgcgcccgac
     1741 atcacgccag cgccaacggt cgcgccgacg gtcgcgcccg tggagacgcc gccttcgagc
     1801 ctgcccccac cgtcaccgtc ggagtccgcg gagccatccg acgaccatgg cgggggatcg
     1861 ggtccggaac catccgatga tcacggcggt ggacctggtc cagaggggac cgatgaccat
     1921 ggcgggaatt ccgggccggg cggcggcggg agcgacgacg gctcgggctc ggacgattcc
     1981 ggctcgggct cgggctcgga ctcttcgact cccaagccga gcgacacccc acgcccgacg
     2041 gggaccccca agccgaccga gacgccgcac taaggcggca gatcaacccg cgtcggcggc
     2101 agtctcggat ccggcctctg cgggcacggt gtcgatatcg aggccgtcac cttgggcggg
     2161 gccgggcctg gcctgggcct tgagcatcga ggcgcggcgc atcgtcgact ccgcgccgcc
     2221 gagcttgatg ccctccgtcg ccacgcccag gatctgcgac cgctgcacga gcgcaaacgg
     2281 atagcgggcg gccagctttc gatcgctgac ccagcgcacg cggacgttcg tcatggggat
     2341 gaacttcggg tccgtggcgt cgacgaacga catgatcgtg ccgtcgccga ggatgaagac
     2401 gtcgccgtca atgacgtggg agcgcgtcag gacaacgagg cgttgggacg tcttctcgat
     2461 gaggtcgcct gcggccccgg tcgatcccga gccgtcagat gccagctgcc cgacgacgga
     2521 gatgtcgtca gggaggacgc gcaactcggg gagggtcaat cgggtcgctc cgccgacacg
     2581 tgtcagcacg acgacgtcgc gaatgaccat gtagccctgc acgatgttga cgaagtcgga
     2641 aacccgccgg aatcgaccga ggttcacgat cccggcaacc cacagctccg tgccgatcag
     2701 ctcgacatac tgctcgccgc cgccctcgcc ctcgtcggca aacgccggcg cgccggtgcc
     2761 ccaggcatcg accgaccgat cgaatgtcgg tggtgcatcg ccccattcgc cc
//
LOCUS       DATBSW010000291         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_521850, whole genome shotgun sequence.
ACCESSION   DATBSW010000291 DATBSW010000000
VERSION     DATBSW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521850"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VIF63_08095"
     CDS             complement(<1..231)
                     /locus_tag="VIF63_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239383.1"
                     /translation="MATDQAELSAAQRAGLAIVAKRQLLLSPVDALFRLHPGDRPLVK
                     AGEAVGRGQPLIEHFRDPRTIVVSGIAEHDGPP"
     gene            complement(231..1859)
                     /locus_tag="VIF63_08100"
     CDS             complement(231..1859)
                     /locus_tag="VIF63_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239384.1"
                     /translation="MTNVPRALLAIDTGAATTSVALLGKPGVRWRLLGSMSAPAGTPV
                     EALAEILAARLVEADHGLAEQVGVAVATVAELPRLEARGEPPSTLAVLGASRRSVGLL
                     EAVAARTPWRVRAASPESHDPREMTELALRPEVSAVLVGAGDPPGPDERGWLDDLSGL
                     VGAVARRRPELRVVLAGPVAARPAWAEGFGGPLEAAADRVFEGPAIGLRSGPDEPLRE
                     ILERLLPETNDTRHMAVRAVLSLADALDRRLEVVEIGLDGGLRAMATPGVADQGPVAV
                     AVRTARAALVPADVDDSAVDAVLAWTTGSLDRHRMGDRLLDLRMQPWADASGEGARLR
                     LAAARAALTRLAAHSTDIGAMASPDVTIIAGGAFAVAPARAVALAIADTVRRTGATQL
                     AIDHARLLGPIGTIDDPDERRGLVADLADDLLAPIGSLLVVGGVPFSRRSTGRAGRLT
                     LDGDRHGRHDLQGGEIAFVDLAPGDRALATLDFRDAVRVGHRARRVAVSVSGGVAGLA
                     IDLRDVPLRLPERRDRRRSALASWGELAWPGDER"
BASE COUNT          256 a          723 c          723 g          206 t
ORIGIN      
        1 aggcggcccg tcgtgctccg cgatgccgga caccacgatc gtgcgcgggt cccggaagtg
       61 ctcgatgagc ggctggccgc ggccgaccgc ttccccggcc ttcacgagcg gccgatcgcc
      121 gggatggagc cggaacaggg catcgaccgg cgacagcagg agctggcgct tcgccacgat
      181 cgcgaggccc gctcgctggg cggcgctgag ctccgcctgg tccgtcgcca tcatcgctcg
      241 tcaccgggcc acgcgagctc gccccacgag gcgagcgccg agcggcgccg gtcgcggcgc
      301 tccggcagcc ggagcggcac gtcccgcagg tcgatggcca ggccggccac gccgcccgag
      361 acagacacgg cgacccgtcg cgcccgatgc ccgacccgca ccgcgtcgcg gaaatccagc
      421 gtggccagcg cccgatcacc cggcgccaga tccacgaacg caatctcgcc accctgcagg
      481 tcgtggcgac cgtggcgatc gccgtcgagc gtgagccggc cggcgcgacc ggtgctccga
      541 cgcgagaagg ggacgccgcc gacgacgagc aggctgccga tcggggccag aaggtcgtcg
      601 gcgaggtcgg ccacgagccc gcgacgctcg tccgggtcgt cgatcgtgcc gatcggcccg
      661 agcaggcggg cgtggtcgat cgcgagctgc gtcgcaccgg tgcggcggac cgtatccgca
      721 atcgcgaggg ccacggctcg agcgggcgcc accgcgaacg caccgcccgc gatgatcgtc
      781 acgtccgggc tcgccatcgc cccgatgtcc gtgctgtggg cggcgagccg cgtgagggcg
      841 gctcgcgctg cggcgagacg gagccgggcg ccctcgcccg acgcgtcggc ccacggctgc
      901 atgcgcaggt cgagcaggcg gtcgcccatg cgatgccggt cgaggctgcc cgtggtccat
      961 gccagcaccg cgtcgacggc cgaatcgtcg acgtcggcgg gcacgagcgc ggcgcgcgcg
     1021 gtccgaaccg cgaccgcgac cggcccttga tcggcgacgc cgggcgtcgc catcgcccgc
     1081 agcccgccat cgagcccgat ctcaaccacc tcgaggcggc gatcgagcgc gtcggccagc
     1141 gagagcaccg cgcgcacggc catgtggcgc gtgtcgttgg tctcgggcag gagccgctcc
     1201 aggatctcgc gcagtggctc atccgggccg ctgcgcaggc cgatggcggg gccctcgaag
     1261 acgcgatcgg cggccgcctc gagcggacct ccgaagccct ccgcccaggc cggccgtgcc
     1321 gcgacaggac cggcgagcac gacgcggagc tcgggccggc ggcgcgccac cgcaccgacg
     1381 agcccggaca ggtcatccag ccagccgcgt tcgtcgggtc cgggtgggtc gcccgcgccg
     1441 acgaggaccg ccgacacctc ggggcgcagg gcgagctcgg tcatctcgcg cgggtcgtgg
     1501 ctctccggcg acgcggctcg gacgcgccac ggtgtgcgcg cggcgacggc ttcgagcagc
     1561 ccgacggatc ggcgcgaggc gccgagcacg gccagggtcg acggtggctc gccgcgcgcc
     1621 tccaagcggg gcagctcggc gacggtggcg acggcgacgc cgacctgctc ggcgagccca
     1681 tggtcggctt cgacgagccg ggctgcgagg atctcggcga gggcttcgac cggcgtcccg
     1741 gcgggcgccg acatcgagcc cagcagccgc cagcgcacgc ccggcttgcc gagcagggca
     1801 accgacgtgg tcgccgctcc ggtatcgatc gcgaggagcg cgcggggaac gttcgtcact
     1861 tggtcatgct accggcaaga accgccgtcg gccctcgcga gcggcgtc
//
LOCUS       DATBSW010000292         5928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1155614, whole genome shotgun sequence.
ACCESSION   DATBSW010000292 DATBSW010000000
VERSION     DATBSW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155614"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VIF63_08105"
     CDS             <1..804
                     /locus_tag="VIF63_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239385.1"
                     /translation="DSLSLESSAPLWSRILTEVSQGMPIAKFQRPEGLVDVEVDAISG
                     LLPGNGTVKTVKEMFIEGTEPTRKDNLHVQAEIDEATGLLWQDGCTGPKSTRLFLDFS
                     QAEERFPAWQPYTQEWADRAARGAGVRGGPKKTRTMYFYSLGFHPFGATWGGRFKPTE
                     VCQPIVHCPPEGGPPTPEPSVVIPCVPDPTEQPTDSGGPPPTKPGNGNGKTPKPTISL
                     PSLAPGLDGAASTAAFEPLAFFPLLVPFAGVAIANRFKPKRPARAKRRR"
     gene            complement(850..3288)
                     /locus_tag="VIF63_08110"
     CDS             complement(850..3288)
                     /locus_tag="VIF63_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEY8239386.1"
                     /translation="MDSRSIAILEFPLVRERLAERTSFGPSRRLAEGLTPDADPVVVK
                     RRLDETDEARGLVEERPGSGIAGARDIGPAIERAARGGRLEPQQFLDVWSTLDATAAL
                     KPHLADARRPLQRELGRLLHPLPAITSTLARSFDPAGELLDTASPRLGGLRAAVRVAF
                     DRLKRRLDSLVSSELGNALQEPIVTLRNGRYVVPVRAEAKGRVKGIVHDASGSGQTLF
                     IEPLVVVELGNAWREAQAAEQAEVERILDELSALVGANAGPLRETLDALARFDFWAAK
                     ALLAGELGASRAETATRPEVILLSARHPGLTGRVVPIDVRLGDGFTALVVTGPNTGGK
                     TVTLRTLGLLSLMHQAGLHVPAAPGSRLPIFRDVFADIGDEQSIAQSLSTFSGHMRSI
                     IRIVEAAGPGTLVLLDELGAGTDPTEGSALAQALLDHFIKAGALVAATTHYAELKAYA
                     HTTPGSMNASVEFDLETLSPTYRLTIGLPGRSQAFAIAERLGLPVALVSDAKSRLSEA
                     QRTFEATLARISEVETETADALERSRVAEARAADALRTADEERRKARESRTETERVAR
                     DEAERIVAEMRTELEAARRSLERGQLTAPAIDSVLERADERLGRLPVHEAAVKGRGAR
                     AGAKDSGAADGAGAPATPAWRVGDEARSRSGGWTGRIAELDRSKRRATIETGAMRVSV
                     EVADLEPADAASSGSGSGSGSGAGYSSGSPDVSQLAGTNVAQVRLSRARNVPMSLDLR
                     GARVEEALSALDRYLEDASLAGLAKVSVIHGMGTGALRDAVRSDSAAHPLVKAVRAGE
                     RGEGGDGVTVVELG"
     gene            complement(3292..4059)
                     /locus_tag="VIF63_08115"
     CDS             complement(3292..4059)
                     /locus_tag="VIF63_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY8239387.1"
                     /translation="MPVHAGRPGRVRAGADRPALTARNSTFPRELLAGPTLDAARALL
                     GAHLVREARVARIVEVEAYIGEDDAASHARFGRTARNAVMFGPPGIAYVYLVYGMHEC
                     LNVVTEPDGRAAAVLIRAIEPVAGEEAMREARIAHALRRARTHDAREQTRRRIEALAA
                     GRLATGPGLVAAAFDITRADTGADLLDPSSALRIEPGRPVERVVQTPRIGIDYAAEPW
                     RSLPWRFTAAESSDRGHEGASPRSSEGRSQTTPTDTH"
     gene            complement(3999..4589)
                     /locus_tag="VIF63_08120"
     CDS             complement(3999..4589)
                     /locus_tag="VIF63_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY8239388.1"
                     /translation="MERLQTFDLVIFLALLAMFVVGYAQGVVRRLLGIGAIIFSIVLA
                     SQLRDPLGQYLSNQWTTIATNYSHMIAFGAVFFASAVTLSLAIQIMYRPAPLFDKYPV
                     LDEILGGLLGVLEGFIILIAILLTIDPYFTRPEFHDHPGLGEFGLLRSLFDFMNPTLT
                     AAILRENVIPGILAVVGFLFTQDVRDAFARALTVRR"
     gene            complement(4638..>5928)
                     /locus_tag="VIF63_08125"
     CDS             complement(4638..>5928)
                     /locus_tag="VIF63_08125"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239389.1"
                     /translation="QVAFRPARVDRLLGTTLGADAQRDVLRRVGIDTEPAPGAIEIQV
                     ATGSAAYAVPAEPGSAVIATVPTWRRDIEVEADLAEEVARVHGYEEVPPHLPATEMPH
                     WRETPLAARDAIRSALAGIGLTEAVTYGLVSPRMIEAFRWSFEDRPAAGETAAEGRPV
                     TVTNPLSMDHSLLRQSIVGSLVEVVDSNARHGQADVAVFEVGKGYGGVTDDTREWWRL
                     GLALTGAFEPPAWNRPRRDADLEDAKGVIEVIAELLGAGPPEYRPLISEPILHPGRSA
                     TVESRRPNGDVAIAGVVGELHPRLAEAWELRGRRVIVAELSVTGLTGGALPVVQAAPL
                     PRFQPIERDLTVDVADTTPAADVLRHARAAGGAYLQEVVLVGTYRGQPLGPHERSLTF
                     RLRFGAAEAQVRDEEVDAALEAITGALTHHVGARIRS"
BASE COUNT          843 a         2118 c         2186 g          781 t
ORIGIN      
        1 gacagcctgt ccctcgagtc gtccgccccg ctgtggtcgc gcatcctgac cgaggtcagc
       61 cagggcatgc cgatcgccaa attccagcgc ccggagggcc tcgtcgacgt cgaggtcgac
      121 gcaatcagtg ggctcttgcc gggcaacggc accgtgaaga cggtcaagga gatgttcatc
      181 gagggcacgg agcccacgcg caaggacaac ctgcacgtcc aggccgagat cgacgaggcg
      241 accggcctgc tctggcaaga cggctgcacc ggtcccaagt cgacccgcct gttcctcgac
      301 ttcagccagg ccgaggagcg cttcccggcc tggcagccgt acacccagga gtgggcggac
      361 cgcgcggccc gtggcgccgg cgtccgcggc gggccgaaga agacccggac catgtacttc
      421 tacagcctgg gcttccaccc cttcggggcg acgtggggcg gccggttcaa gccgaccgag
      481 gtctgccagc cgatcgtcca ctgcccgccg gaaggcggtc cgccgactcc ggagccgtcg
      541 gtggtcatcc cgtgcgttcc cgatccaact gagcagccca cggactcagg cggtccaccc
      601 ccgaccaagc ccggcaacgg caacggcaag acgccgaagc cgacgatcag cctgccgagc
      661 cttgcgcccg ggctggacgg ggcggcgtcg accgcggcgt tcgagccgct ggccttcttc
      721 ccgctgctcg tccccttcgc cggggtcgcg atcgcgaacc ggttcaagcc gaagcgaccc
      781 gcacgagcga agcgacgccg ctagccccgg cgactgcacg gcgtcagcgc ggtaagcgcg
      841 gctcggcggt cagcccagct cgacgacggt caccccgtcg ccaccctcgc cccgctcccc
      901 tgcccgaacc gccttcacca gcgggtgcgc ggccgaatcc gaccgcaccg catcgcgcag
      961 cgcgcccgtc cccatcccgt gaatcacgct gaccttggcg aggccggcga gcgaggcatc
     1021 ctcgaggtag cggtccagcg ccgacagcgc ctcctcgacc cgcgcgccgc gcaggtccag
     1081 cgacatcggc acgttccgcg cacgcgagag gcgcacctgg gcgacgttcg tgccggcaag
     1141 ctgggacacg tcgggcgacc ctgagctgta gcctgcgccg gatccggaac cggacccgga
     1201 acccgaactg gctgcgtccg ccggctcgag gtcggcgacc tcgacactga cgcgcatggc
     1261 gccggtctcg atcgtcgcgc gccgcttcga ccgatcgagc tcggcgatgc gcccggtcca
     1321 gccgcccgac cggctgcgtg cctcgtcacc gacacgccac gccggggttg caggcgcccc
     1381 tgcaccgtcc gcggcgccgc tgtccttcgc gccggcacga gcgccacggc ctttcaccgc
     1441 ggcctcgtgg acgggcagcc ggcccaggcg ctcgtcggcg cgctcgagca cggaatcgat
     1501 cgccggagcg gtgagctggc cgcgctccag cgaccgtcgt gccgcctcga gctccgtgcg
     1561 catctcggcg acgatccgct cggcctcgtc gcgggccacg cgctcggtct cggtgcgtga
     1621 ctcgcgggcc ttgcgccgtt cctcgtcggc ggttcgcagc gcgtcggcgg cgcgggcctc
     1681 ggcgacccgc gagcgctcca gcgcgtccgc cgtctcggtc tcgacctcgc tgatccgcgc
     1741 gagggtggct tcgaacgtgc gctgggcctc cgacaggcgc gactttgcgt cgctgacgag
     1801 ggccacgggc aggccgagcc gctccgcgat cgcgaacgcc tgtgatcggc cgggcaggcc
     1861 gatcgtgagc cggtacgtcg ggctgagtgt ctcgaggtcg aactcgaccg aggcgttcat
     1921 cgagcctggg gtcgtgtggg cgtaggcctt gagctcggcg taatgcgtcg tcgcggcgac
     1981 gagcgcgccg gccttgatga agtggtcgag cagggcctgt gccagcgccg agccctcggt
     2041 cgggtcggtg ccggcgccga gctcgtcgag cagcacgagg gtgcccgggc cggccgcctc
     2101 gacgatgcgg atgatcgagc gcatgtggcc cgagaacgtc gagagcgact gggcgatcga
     2161 ctgctcgtcg ccgatgtcgg cgaacacgtc gcggaagatc ggcaggcgcg agcccggcgc
     2221 cgccggcaca tgcaggccgg cctggtgcat gagcgacagc agcccgagcg tgcgcagggt
     2281 gaccgtcttg ccaccggtgt tcgggccggt cacgaccagc gcggtgaacc cgtcgccgag
     2341 ccggacgtcg atcgggacca cgcggcccgt caggccgggg tggcgcgccg acaggaggat
     2401 cacctcgggg cgggtcgccg tctcggcgcg cgaagcgccc agctcaccgg cgagcagcgc
     2461 cttcgcggcc cagaagtcga aacgggcgag cgcgtcgagg gtctcgcgca gcggcccggc
     2521 gttcgcgccg acgagcgcgg acagctcgtc gaggatgcgt tcgacctcgg cctgctcggc
     2581 cgcctgcgcc tcgcgccagg cattgccgag ctccacgacc acgagcggct cgatgaacag
     2641 cgtctggccg ctgcccgacg cgtcgtggac gatgcccttg acccggccct tcgcctcggc
     2701 gcggacgggc acgacgtagc gcccgttgcg gagcgtgacg atcggttcct ggagcgcatt
     2761 gccgagctcc gaggagacga gcgagtcgag gcggcgcttc aggcggtcga aggcgacgcg
     2821 aacggcggcg cggaggccgc cgagtcgcgg cgaggcggtg tcgaggagct cgcccgccgg
     2881 atcgaacgac cgtgcgagcg tcgaggtgat cgcgggcagc ggatgcagga ggcggccaag
     2941 ctcgcgctgg agcggccggc gggcgtcggc gaggtgcggc ttgagggcgg ccgtggcgtc
     3001 gagcgtcgac cagacgtcga ggaactgctg gggttcgagc cggccgccgc gggcggcgcg
     3061 ttcgatggcc gggccgatat cgcgcgcgcc ggcaatcccc gagccggggc gctcctcgac
     3121 caggccgcgc gcctcgtcgg tctcgtcgag gcggcgcttg acgacgactg ggtcggcgtc
     3181 gggggtcagg ccctcggcga gacgccggct cgggccgaac gaggtgcgct cggccagccg
     3241 ctcgcggacg agcgggaact ccaggatcgc gatcgagcgg ctatccatcg tctaatgagt
     3301 gtcggttgga gtggtctgcg accgaccctc cgaggaccga ggtgaagccc cttcgtgccc
     3361 tcggtccgag gattcggccg ctgtgaagcg ccagggcagg ctgcgccacg gctccgcggc
     3421 gtagtcaatg ccgatgcgtg gggtctggac gacgcgctcg acgggtcggc cgggttcgat
     3481 ccgcagcgcc gagctgggat cgaggaggtc cgccccggta tcggcccggg tgatgtcgaa
     3541 cgctgcggcg accaggccgg ggcccgtcgc gagcctcccg gcggcgagcg cctcgatgcg
     3601 tcgtcgtgtc tgctcgcgag cgtcatgggt gcgggcccgg cgcagcgcgt gggcgatccg
     3661 cgcctcgcgc atcgcctctt cgccggcgac cggctcgatg gcgcgaatca gcacggccgc
     3721 agcgcggccg tcgggctccg tgacgacgtt caggcactcg tgcatcccgt agacaaggta
     3781 cacgtaggcg atcccgggag gcccgaacat gaccgcgttc cgcgccgttc ggccgaaccg
     3841 ggcgtgggag gccgcgtcgt cctcgccgat gtacgcctcg acctcgacga tccgcgcgac
     3901 tcgcgcctcc cgaaccaggt gcgcgcccag cagggcgcga gccgcgtcga gggtcgggcc
     3961 ggcgaggagc tcgcgcggga acgtggagtt gcgcgcggtc agcgccggac ggtcagcgcc
     4021 cgcgcgaacg cgtcccggac gtcctgcgtg aacaggaatc cgaccaccgc gaggattccc
     4081 gggatcacgt tttcgcggag gatcgcggcg gtcagcgtcg ggttcatgaa gtcgaacagc
     4141 gagcgcaaca gcccgaactc accgaggccc ggatggtcgt ggaactccgg acgggtgaag
     4201 tacgggtcga tcgtcagcag gatcgcgatc aggatgatga aaccttcgag cacgccgagg
     4261 agcccgccga ggatctcgtc gaggacgggg tacttgtcga acagcggcgc aggccggtac
     4321 atgatctgga tcgcgaggct cagcgtgacc gccgacgcga agaacaccgc accgaacgcg
     4381 atcatgtggc tgtagttggt cgcgatggtc gtccactggt tcgacaggta ctggccaagc
     4441 ggatcacgta actggctggc gaggacgatc gagaagatga tcgccccgat gccgaggagg
     4501 cgccggacga ccccctgcgc gtagccgacg acgaacatcg ccagcagcgc caggaagatc
     4561 accaggtcga acgtctggag ccgctccacg tgtgactacc tccccggggt ggtgtggacg
     4621 gcggcagcgt agcaggctca gctgcggatt cgggctccca cgtggtgcgt caacgcgccg
     4681 gtgatggcct cgagcgccgc gtcgacctcc tcgtcgcgaa cctgcgcctc ggcggcgccg
     4741 aagcggagcc ggaacgtcag gctgcgctca tgcgggccca gcggctggcc gcggtacgtc
     4801 ccgacgagca caacctcctg cagataagcg ccgcccgcgg cgcgcgcgtg ccgcaggacg
     4861 tcggccgcgg gggtcgtgtc ggcaacgtcg accgtcaggt cgcgttcgat cggctggaag
     4921 cggggcagcg gcgccgcctg gacaacgggc agcgcgccac cggtgaggcc cgtcaccgac
     4981 agctcggcga cgatcacccg ccggccgcgg agctcccagg cctcggcgag ccgcggatgc
     5041 agctcgccga cgaccccggc gatggccacg tcgccgttcg gacgccggga ttcgacggtc
     5101 gcgctgcgcc cgggatggag gatcggctcc gagatgagcg gccggtactc cggcgggccc
     5161 gcgccgagca gctcggcgat cacctcgatc acgcccttcg cgtcctcgag gtcggcgtcg
     5221 cggcgcggcc ggttccacgc cggcggctcg aacgcgcccg tcagggcgag cccgaggcgc
     5281 caccactccc gcgtgtcgtc cgtgacgccg ccgtagccct tgccgacctc gaagacggca
     5341 acatcggcct ggccgtggcg ggcgttcgaa tcgacgacct cgacgaggct gccgacgatc
     5401 gactggcgca ggagcgagtg gtccatcgac agcgggttgg tcacggtcac cgggcggccc
     5461 tcggcggcgg tctcgccggc cgccgggcga tcctcgaacg accagcggaa cgcctcgatc
     5521 atccgcggcg agacgagccc gtacgtgacg gcctcggtga gcccgatccc ggcgagcgcg
     5581 ctccggatcg cgtcccgagc agcgagcggg gtctcgcgcc agtggggcat ctcggtcgcg
     5641 ggcaggtgcg gcggcacctc ctcgtagccg tggacgcggg cgacctcctc ggcgagatcc
     5701 gcctcgacct cgatgtcgcg gcgccacgtc gggaccgtcg cgatcacggc ggagcctggt
     5761 tcggcaggca ccgcgtatgc cgcggagccc gtggcgacct ggatctcgat cgcgcccggc
     5821 gccggctccg tgtcgatgcc gacccggcgc agcacgtcgc gctgggcatc ggcgccgagc
     5881 gtcgtcccga ggagccggtc aacgcgcgcg ggccggaacg cgacctgg
//
LOCUS       DATBSW010000293         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_557128, whole genome shotgun sequence.
ACCESSION   DATBSW010000293 DATBSW010000000
VERSION     DATBSW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557128"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VIF63_08130"
     CDS             <1..904
                     /locus_tag="VIF63_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY8239390.1"
                     /translation="VRMILVDLKRVELAAYNGLPHLLVPVITETEKAKAALKWAVNEM
                     EGRYRRFAGATARNIKAYNESRADPDDKMPYIVIIIDELADLMMREGKHVEEPIVRLA
                     QKARATGIHMVLATQRPSVNVVTGLIKANFPSRIAFAMASQIDSRTILDTPGAEDLIG
                     RGDMLYQPSDLPRPMRLQGVFVSDHEIGKVTSHWKDQIEDPHYDMAILQSDEEGTASS
                     DDLGDEDADRLLPDAVEVIREYDRASASLLQRRLKVGYARAARMIDQLEQRGYIGSFD
                     GSNARVVLRRDTTDHGNGGDPGDD"
     gene            908..>1753
                     /locus_tag="VIF63_08135"
     CDS             908..>1753
                     /locus_tag="VIF63_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8239391.1"
                     /translation="MTTRDHARRAAGRLGAGEKGEPAESGPGLPDRLAAARERKGVDL
                     NRAERDTKIRVRYLSALERGDYRDLPGAVYTKGFLRNYAIYLNLDPEDVLRQWRRERG
                     DQVGEAPIIVPPRPIDEPKRPLNFSPSVVVAALLTAGVILFFVYLSSQLLRYSRPPEL
                     AVTNPATAVVSVNEDTTSYRLAGTSSPGATVTIKAAGQTQPIRVTALASGDWSVQVDL
                     RRGQNRFDIDALNPETGKSAEVTRTIVITVPFLVFQAPTLVVSQPVEGTTYGNGAIAV
                     EGTTTN"
BASE COUNT          313 a          611 c          577 g          252 t
ORIGIN      
        1 cgtccggatg atcctggtcg acctcaagcg ggtcgagctg gccgcttaca acggcctgcc
       61 ccacctgctc gtgccggtca tcaccgagac cgagaaggcc aaggcagccc tgaagtgggc
      121 ggtcaacgag atggagggcc gataccggcg ctttgccggg gccacggccc gcaacatcaa
      181 ggcctacaac gagagccgcg ccgatcccga cgacaagatg ccgtacatcg tgatcatcat
      241 cgacgagctc gccgacctga tgatgcgcga gggcaagcac gtcgaggaac cgatcgtgcg
      301 gctcgcccag aaagcccgtg cgacgggcat ccacatggtc ctggcaacgc agcggccctc
      361 ggtcaatgtg gtcacgggcc tgatcaaggc gaacttcccg tcacgaatcg ccttcgccat
      421 ggcgtcccag atcgactcgc gcacgatcct cgacacgccg ggtgccgagg acctcatcgg
      481 gcgcggcgac atgctctacc agccatccga cctgccccga ccgatgcgcc tccagggcgt
      541 gttcgtctcc gatcacgaga tcggcaaggt caccagccac tggaaagacc agatcgagga
      601 cccgcactac gacatggcga tcctccagag cgacgaagaa ggcacggcgt catcggacga
      661 cctcggcgac gaggatgccg atcgattgct gccggacgcc gtcgaggtga tccgggaata
      721 cgatcgcgcc tccgcgtcgc tgctccagcg gcgcctgaag gtgggctatg cccgggcggc
      781 ccggatgatc gaccagctcg agcagcgcgg ctacatcggg tcgttcgatg ggtcgaatgc
      841 ccgagtggtg cttcggcgcg acaccaccga tcatggcaac ggtggcgatc cgggcgatga
      901 ttgaccgatg acgacccgcg atcacgcacg ccgtgctgcc ggtcgtctcg gcgcgggcga
      961 gaagggtgag ccggctgaga gtggccccgg cctgcccgat cgactcgcgg cggctcgtga
     1021 gcgcaaaggg gtcgatctca accgcgccga gcgcgatacg aagatccgcg tccgctacct
     1081 gagtgcgctc gagcgcggcg actaccgcga cctgccgggc gccgtgtaca cgaagggctt
     1141 tctgcgtaac tacgcgatct acctgaacct cgatcccgag gacgtcctcc ggcagtggcg
     1201 ccgcgagcgc ggtgaccagg tcggggaggc gccgatcatc gtgccgccgc ggcccatcga
     1261 cgagccgaag cggcccctca acttctcgcc gtcggtcgtc gtcgcggcgc tgctgactgc
     1321 cggcgtgatc ctgttcttcg tctacctgtc gtcgcagctg ctgcgctact cccgaccgcc
     1381 ggagctcgcc gtgacgaacc cggcgaccgc ggtcgtctcg gtcaacgagg acacgacctc
     1441 ctaccggctc gcgggcacgt cgagccccgg cgcgaccgtg acgatcaagg cagccggcca
     1501 gacccagccg atccgcgtca ccgccctcgc gagcggcgac tggagcgtcc aggtcgacct
     1561 gcgacgtggc cagaaccggt tcgacatcga cgcgctgaac ccggagacgg ggaagtcggc
     1621 cgaggtgacg cggacgatcg tgatcaccgt gcccttcctg gtgttccagg cgccgacgct
     1681 cgtcgtgagc cagcccgtcg aaggcacgac ctacggcaac ggcgcgatcg cggtcgaagg
     1741 cacgacgacc aac
//
LOCUS       DATBSW010000294         9163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_945152, whole genome shotgun sequence.
ACCESSION   DATBSW010000294 DATBSW010000000
VERSION     DATBSW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945152"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VIF63_08140"
     CDS             <1..407
                     /locus_tag="VIF63_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY8239392.1"
                     /translation="IALPALLLFLIVFYWTPPHFWALALRIRGDYAAAGVPMLPVVRG
                     IAETSRQIGLYTILLVALTAVFAVVAQMGLIYAVAAAVLGALFLWRAWILWRQGTSPE
                     ASTAQAIRLYRYSITYLAVLFAAVALDTIWRV"
     gene            466..1074
                     /locus_tag="VIF63_08145"
     CDS             466..1074
                     /locus_tag="VIF63_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239393.1"
                     /translation="MSLHIPIQRARAALALAFLALPAILASPAITLADCMMPPPIEEA
                     VKSAEIVFLGTVTNTANRDSWATVEIEEVWVGPDMPAEVLIKGGPGGNAATSVDRSFQ
                     VGLKYLFFPYIDEGGEVRPGEVPELAGGLADNSCTSTQQWSPELEALRPADVRAPLGS
                     STTPEAGIDIDLGGILGPIVAGVVVASVLLGIGLLARGRQTT"
     gene            1137..2804
                     /locus_tag="VIF63_08150"
     CDS             1137..2804
                     /locus_tag="VIF63_08150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8239394.1"
                     /translation="MQIAFLGGASTVTGSRFLLTTDRARILIDCGMFQGSPNETVRNR
                     VPLGFDPTELDAILVTHAHLDHCGLLPVAVKEGYKGPIYLTTATAELVELVLLDSGRL
                     QEEFAKRHNRWERRHADDAAKADAKSEAKYREAVEEARRAEAEGHVPTSIEPTGGPGA
                     TGPENAAPAGESDAVAAEEDAAPPSGDVEADLRAMPAMLDIDLDAPLYTEADAKLVMP
                     LFERARYGHELQVAPGILATFQDAGHILGSAIIKLEIRETQGGEPTTIVFSGDLGRPD
                     TPIIRDPTVMSAADYVIVESTYGGREHEPGAEATRMLAEAVRATADAGGVLLIPSFAI
                     GRTQEVVWELDRLIDRGEIPMLPLYLDSPMASKASDIYRRHPEFYDEETAKLLAKGDT
                     PLDYPDQHITRSVDDSKAIATADRPYMIVASNGMLTGGRVVSHLRNLIGDPKATILFV
                     GYQGEGTLGAHLQAGAKTVRLDGQVRDVRCRARSISGFSAHADESELLDWLSNFRDGR
                     KPGDAGYPRTVFLVHGDPEAQLAIRPKVEALGFNVHVPHWRESLTLD"
     gene            2815..4353
                     /locus_tag="VIF63_08155"
     CDS             2815..4353
                     /locus_tag="VIF63_08155"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY8239395.1"
                     /translation="MRPRIQVLDSELVASIVGEAKQLLAEVGMEIRGKVLRERLLELG
                     LKSDATGTRLLFPTDVVERAIASAPHSFTLYDRDGNPHADLGGDRVHFVPGSSGLKIL
                     DHRTGEARLADTKDFVEYVRIADGLEHIPYLATAFSTNNDIEAQVSDAWRLHLVLTNT
                     KRPVVSGAFTEHGVERMVEMMALFRHDRADLVAKPMSIFTITASGNFRYSEDSCQNLL
                     DCVEAGIAVEIVPVTLMGLIAPVTVVGATVFHTVDVLAGITMAQLIKPGAPVLFGGAP
                     ATFHMKIASSPMSAVEALQLDVAYVEVAKSLGFVTQSYMGLSEAKVIDAQAGAETFGG
                     ALLAALAGVNSVSGPGMLDFLLVFSLPKLVLDDELCGQALRFVREVKTVEDLPARALV
                     DQVLSEQHLIMADHTTRNWPTELYLPGRVFDRENRENWTKAGAHDANERAIAEVERKL
                     AAYELVDTDPRAVAELERIIRAGFVAQESLPEIPPPPDAASARAAVVAGAGPARRSNP
                     RRGR"
     gene            complement(4350..5195)
                     /locus_tag="VIF63_08160"
     CDS             complement(4350..5195)
                     /locus_tag="VIF63_08160"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8239396.1"
                     /translation="MDHAAAHERIEDLILEPGRLAALADSIEPDDVALREHLAGCPTC
                     SADLDGWERLQARLTEALPHSADAAAAAVVPVELPPSLRARVISAARSDARARSGRRT
                     GDPAQATLRIARLRSRRAVATWVGLAASLVVLVGATIITVDQVARRSTAEAEARAISA
                     ALSAVDRVLAVPDHRISELRTQGGVSAGSVSWSRHDWVVLTTALAKPSSDQRYRCWLE
                     NGDSSVAIGEMDFVGDTAFWVASLNEWATWELGPTTKFVVTLEPADGSARTGPTVLAA
                     DLGSS"
     gene            complement(5210..5821)
                     /locus_tag="VIF63_08165"
     CDS             complement(5210..5821)
                     /locus_tag="VIF63_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8239397.1"
                     /translation="MIAYDKHASFVFGSVSRFLGDRDAAAEVVQDAFLTLWRRAAQFD
                     ARSGSLLTWLLAIARHRAIDRLRAEGRRPSRDAIRLDSLTSEAGTGAGVSRPDVPPEL
                     VADATTDPGTVSSRRWLQSVVRTGIAELPDHEREVILLGYAQDLSQAQIAERLAWPLG
                     TVKSRTRRALAQLRVHLSGIPDAEDLPAAAFERSAGASQGRDR"
     gene            complement(5911..6498)
                     /locus_tag="VIF63_08170"
     CDS             complement(5911..6498)
                     /locus_tag="VIF63_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239398.1"
                     /translation="MERTRTGSASAQGRLGRGAAIFAASLALVAACAAPGAGPTTAPV
                     SAAPPAATTAPMAGVTLAISQSPTLGAYVAGEGGKSLYVFSKDSAGKSACSGGCATNW
                     PPLIVANAADAHAGAGVTGAIATITRDDGKLQVTLGGAPLYYYAADAAAGDTNGQGVG
                     GFWFLASAAGTPVSGEPAAATDNPAATACGGPACY"
     gene            complement(6574..7500)
                     /locus_tag="VIF63_08175"
     CDS             complement(6574..7500)
                     /locus_tag="VIF63_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8239399.1"
                     /translation="MRIATWNVNSMKARLEKIEWWLDRAAPDVLLMQETKLSDADAPL
                     MTFQMAGYQLVHHGEGRWNGVAIAVRDGIAIDDPITNFGDGPVRDSGAGASVSLEEED
                     FDPSDEARMLSIGVTEPGGDPLRVVSLYAPNGRVVDSAFYAGKLRWFARARRWLDAAR
                     DPDEALLIGGDMNIAPADIDVWDARAVHGGTHVSEPEREAFRNLLAWGLHDAFRERQP
                     EARGRFTWWDYRAGNFHKNFGMRIDHLLPTSSVADRVVAVEIDREARKGKPIPSDHAP
                     LVVDLDTPGKAFDPGWDDAEARIVARGGLRPR"
     gene            complement(7538..7798)
                     /locus_tag="VIF63_08180"
     CDS             complement(7538..7798)
                     /locus_tag="VIF63_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8239400.1"
                     /translation="MMVWFTVELEDKPGSLAKVATALGAAGVNITGIVGVAEDTEGAL
                     MLTTSDADRTRAAFTALGIEFEEHGEGGPDMDAMTIAGVRGR"
     gene            7870..8268
                     /locus_tag="VIF63_08185"
     CDS             7870..8268
                     /locus_tag="VIF63_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEY8239401.1"
                     /translation="MAPPKPPAGPIVQIFGTPDSQPTRACLRCFKERRLEVHLVDIRR
                     KPMAPGELRRFVERLGARALADTEGKPWRDAGLGYLSMSDADLGGRLLADQRLLNLPL
                     VRAGNAFAAGRDEAAWKRLAADATATVAPR"
     gene            8265..>9163
                     /locus_tag="VIF63_08190"
     CDS             8265..>9163
                     /locus_tag="VIF63_08190"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8239402.1"
                     /translation="MSGRAAAQLEWPQVLAWRLRRQLLDPIGTEPVEGVVGRLGAIQA
                     TNGDLAIRVRRSSSSAGDLAQALADGRVISSFAFRGATHLMTPESAGAYLALRKSARM
                     FELPSWQRYYGLLPSDWDGLRAVVREALAAGPKTVDEIVAAIGASPRFAHLGPILAGN
                     PWSVMKAIAWHGDMSFGQARGRQSAFQLLDRNPLWPGLSEVGDAATWAIEAYLRAYGP
                     ATPKHLQYWLGAGLGAARKLLQGATATLRDRLVEVQVEGQPCLAMTDDLDELVATRPS
                     SVVRLLPAYDQWVLGPGTADPHVV"
BASE COUNT         1319 a         3317 c         3201 g         1326 t
ORIGIN      
        1 acatcgccct gcccgcgctc ctgctgttcc tgatcgtctt ctactggacg ccgccgcact
       61 tctgggcact ggccctgcgg atccgcggcg actacgccgc cgccggcgtg ccgatgctcc
      121 cagtcgtgcg cggtatcgcc gagacgagcc gccagatcgg gctgtacacg atcctcctgg
      181 ttgcgctcac cgcggtgttc gcggtcgtgg cccagatggg cctgatctac gcagtcgccg
      241 ccgcggtcct cggtgcgctg ttcctctggc gcgcctggat cctgtggcgg cagggaacct
      301 cgcccgaggc ctcgaccgcc caggcgatcc gcctctaccg ctactcgatc acttatctgg
      361 ccgtgctctt cgccgcagtc gcgctcgaca cgatctggcg cgtctaggga tcggcccagg
      421 gcccggtcgc ggaaccgacg acccaccacg agcgtcccgc cgccgatgag cctacacatc
      481 cccatccaac gtgctcgcgc cgcgcttgcg cttgctttcc tcgcactacc cgcgatcctc
      541 gcgtcgcccg ccatcacgct cgccgactgc atgatgccgc cgccgatcga ggaggccgtg
      601 aagtctgccg agatcgtgtt cctcggcacc gtgaccaaca ccgcgaaccg cgacagctgg
      661 gccaccgtgg agatcgagga ggtctgggtc gggccggaca tgccggccga ggtcctgatc
      721 aagggtgggc cgggcgggaa cgccgccacg tcggtcgacc gctcgttcca agtgggcctg
      781 aagtacctgt tcttcccgta catcgacgag ggcggcgagg tgcgacctgg cgaagtgccc
      841 gagctggcgg gcggcctcgc cgacaacagc tgcacgtcga cccagcagtg gtcgccggag
      901 ctcgaggcgc ttcgcccggc cgatgtccgt gcgcccctcg gctcgtcgac gacgcccgag
      961 gcagggatcg acatcgacct cggtggcatc ctcggcccga tcgtcgcggg cgtggtcgtc
     1021 gcgtcggtgc tgctcgggat cgggcttctc gctcggggtc gccagaccac ctagactcgg
     1081 tccgtgctcc ggaccgcgcc gccgcgtcac ctcgctcccg ccttcgcgcc ctgacggtgc
     1141 agatcgcctt cctcggtggc gcgagcacgg tcaccggctc gcgcttcctc ctgaccacgg
     1201 accgcgcccg catcctgatc gattgcggga tgttccaggg cagcccgaac gagacggtcc
     1261 gcaaccgggt cccgctcggt ttcgacccga ccgagctcga cgcgatcctc gtcacccacg
     1321 cccacctcga ccactgcggc ctcctgccgg tcgcggtgaa ggagggctac aaagggccga
     1381 tctacctcac gaccgcgacg gccgagctgg tcgagctcgt cctcctcgac agcggccggc
     1441 tccaggagga gttcgcgaag cgccacaacc gatgggagcg gcgccacgcc gacgacgcag
     1501 cgaaggccga cgcgaagtcc gaggcgaagt accgcgaggc cgtcgaggag gcgcggcgag
     1561 ccgaggccga aggccacgtc ccgacatcga tcgagccgac cggggggccc ggcgcgacgg
     1621 gccccgagaa tgccgcgccc gcgggcgaat cggacgcggt cgcagctgag gaagacgccg
     1681 cgccgccgtc cggcgatgtc gaggccgacc tgcgggcgat gccggcgatg ctcgatatcg
     1741 acctcgatgc gccgctctac accgaggccg acgcgaagct cgtcatgccg ctgttcgagc
     1801 gcgcccggta cggccacgag ctgcaggtcg cgccgggcat cctcgccacg ttccaggacg
     1861 ccggccacat cctcggctcg gcgatcatca agctcgagat ccgcgagacc cagggtggcg
     1921 agccgacgac catcgtcttc tcgggcgacc tcggccggcc cgacacgccg atcatccgcg
     1981 acccgaccgt catgtccgcc gccgactacg tgatcgtcga atcgacctac ggcggacgcg
     2041 agcacgaacc gggcgctgag gcgacgcgga tgctcgccga ggcggttcgg gcgacggctg
     2101 acgccggcgg cgtgctcctg atcccgtcgt tcgcgatcgg gcgcacccag gaggtcgtct
     2161 gggagctgga tcgactgatc gatcgcggcg agatcccgat gctcccgctc tacctcgact
     2221 cgccgatggc atcgaaggcg tccgacatct accggcggca tcccgagttc tacgacgagg
     2281 aaaccgcgaa gctgctcgcc aaaggcgaca cgcccctcga ctacccggac cagcacatca
     2341 cccgctcggt cgacgattcg aaggcgatcg cgacggccga tcggccgtac atgatcgtgg
     2401 cctcgaacgg catgctgacc ggcggccggg tcgtctcgca cctgcgcaac ctgatcgggg
     2461 acccgaaggc cacaatcctg ttcgtcgggt accagggtga gggcaccctc ggtgcgcacc
     2521 tccaggccgg cgcgaagacc gttcgcctcg acggccaggt tcgcgacgtt cgctgccgcg
     2581 cccgctcgat cagcggcttc tcggctcacg ccgacgagtc cgagctcctc gactggcttt
     2641 cgaacttccg ggacggacga aaacccggcg acgccggata ccctcggacg gtgttcctcg
     2701 tccacggcga tcccgaggcg cagctcgcga tccggcccaa ggtcgaagcg ctcggcttca
     2761 acgtgcacgt cccgcattgg cgggaaagcc tgaccctgga ctgaggtccg cgccatgcgc
     2821 ccccggatcc aggtcctcga ctccgagctg gtcgcgtcga tcgtcggcga ggcgaagcag
     2881 ctgctggccg aggtcgggat ggagattcgc ggcaaggtcc tccgcgagcg cctcctggag
     2941 ctcggcctca agagcgacgc gaccggcacg cgcctcctgt ttccgaccga cgtcgtcgag
     3001 cgggcgatcg cgtcggcgcc gcattcgttc acgctgtacg accgcgacgg caacccgcac
     3061 gcggacctcg gcggcgaccg cgtccacttc gtccccggct cgagcggcct gaagatcctc
     3121 gaccaccgga ccggcgaggc gcggctcgcg gacacgaagg acttcgtcga atacgtccgg
     3181 atcgcggacg gcctcgagca cattccgtat ctcgcgaccg cgttctcgac gaacaacgac
     3241 atcgaggcgc aggtctccga cgcctggcgc ctccacctcg tgctcacgaa caccaagcga
     3301 ccggtggtgt ccggcgcgtt caccgagcac ggtgtcgagc ggatggtcga gatgatggcg
     3361 ctcttccgcc acgatcgagc cgacctcgtc gcgaagccga tgtcgatctt cacgatcacg
     3421 gcgtcgggca acttccggta cagcgaggat tcgtgccaga acctgctgga ctgcgtcgag
     3481 gccgggatcg cggtcgagat cgtcccggtg acgctcatgg gcctgatcgc gccggtgacc
     3541 gtcgttgggg ccacggtctt ccacaccgtc gacgtgctcg ccgggatcac gatggcccag
     3601 ctcatcaagc cgggtgcgcc ggtgctgttc ggcggggcgc cggcgacgtt ccacatgaag
     3661 atcgccagct cgccgatgtc ggcggtcgag gcgctccagc tcgatgtcgc ctacgtcgag
     3721 gtcgcgaagt cgctcgggtt cgtcacgcag tcatacatgg gcctgtccga ggccaaggtc
     3781 atcgacgcgc aggcgggcgc ggagacgttc ggcggcgcgc tgctggcggc gctcgcgggc
     3841 gtgaactccg tgtccggccc cggcatgctc gacttcctcc tggtcttctc gctgcccaag
     3901 ctcgtgctcg atgacgagct ctgcggccag gcgctgcgct tcgtccgcga ggtgaagacg
     3961 gtggaggacc tgccggcgcg cgcgctcgtg gaccaggtcc tgtccgagca gcacctgatc
     4021 atggccgacc acaccacccg caactggccg accgagctgt acctgcccgg ccgcgtcttc
     4081 gatcgcgaga accgcgagaa ctggacaaag gcaggcgcgc acgatgccaa cgagcgggcg
     4141 atcgcggagg tcgagcgcaa gctcgccgcg tacgagctcg tggacacgga tccgcgggcg
     4201 gtggcggagc tcgagcggat cattcgcgcc ggcttcgtcg cccaggaatc gctgcccgaa
     4261 atcccgcccc cgccggatgc cgcatcagcg cgcgccgccg tggtggccgg cgccggcccg
     4321 gcccgccgat cgaacccgag gcgcgggcgc tagctcgagc ccagatctgc cgccaggacc
     4381 gtggggccgg tacgcgcgga gccgtccgcc ggttcgaggg tgaccacgaa cttcgtcgtc
     4441 ggacccagct cccaggtcgc ccattcgttg agcgaggcga cccagaacgc cgtgtcgccg
     4501 acgaagtcca tctcgccgat ggccacgctc gagtcgccgt tctcgagcca gcagcggtag
     4561 cgctgatcgg acgacggctt cgcgagcgcc gtggtcagca cgacccagtc gtggcgcgac
     4621 cacgagaccg agccggcgga cactccgcct tgcgtacgca gctcgctgat gcggtggtcg
     4681 ggcacggcca gcacccgatc aacggcggaa agtgccgcgg aaatggctcg cgcctcagcc
     4741 tcggcggtcg aacgtcgtgc gacctggtcg accgtgatga tcgtggcgcc gacgaggacg
     4801 acgagcgatg ctgccagccc gacccacgtc gcgacggcgc gccgagatcg aagccgcgcg
     4861 atgcggaggg tcgcctgcgc agggtcaccc gtacgccggc cagagcgggc gcgcgcatcg
     4921 ctgcgcgccg cggagatgac gcgggcccgg agcgagggcg gcagctcgac cgggacgacc
     4981 gcggccgccg cggcatcagc ggagtggggg agcgcctcgg tcagcctggc ctggagtcgc
     5041 tcccagccgt cgaggtcggc gctgcacgtc gggcagccgg ccaggtgctc gcgcagggcg
     5101 acgtcgtcgg gctcgatcga gtcggcgagg gccgccaggc gacccggctc gaggatcagg
     5161 tcctcgatcc gctcgtgggc cgccgcgtga tccatcggct ccatcgagct catcggtccc
     5221 gcccctggga tgccccggcg ctgcgttcga acgccgccgc cggcaggtcc tctgcgtcag
     5281 ggatgcccga caggtggacc cgcagctggg cgagcgcgcg ccgggtgcgc gacttgaccg
     5341 tgccgagcgg ccacgccagc cgctcggcga tctgggcctg ggacaggtcc tgggcgtagc
     5401 cgaggaggat cacctcgcgc tcgtgatcgg gcagctcggc aatcccggtg cgaaccacgg
     5461 actggagcca gcgacgcgac gagaccgtgc cgggatcggt tgtggcgtcg gcgaccagct
     5521 ccggtggcac gtccggtcgc gagacgccgg cgcctgtccc tgcctcggac gtgagcgaat
     5581 ccaggcggat ggcgtcgcgc gacgggcgcc ggccctcggc gcgcagtcgg tcgatggccc
     5641 gatggcgggc gatcgccagc aaccaggtca ggagcgaccc ggaccgggcg tcgaactgcg
     5701 cggcgcgccg ccacagggtg aggaacgcgt cctggacgac ctcggccgcg gcgtcgcggt
     5761 cgccgaggaa gcgcgacacc gagccgaaga cgaaggacgc atgcttgtcg taggcgatca
     5821 cgaacgctgc ctcatcaccg gttgcgattc ggccaacaag gcgtgcgtcg tcgggttctc
     5881 cctcgtccgt gtcgagagcc cgacgaccgc tcaatagcag gcggggccgc cacaagcggt
     5941 cgcggcggga ttgtctgtcg cagccgcggg ctcgccgctc acgggcgtgc cggccgcgga
     6001 agcgaggaac cagaagccgc cgacaccctg gccattggtg tcgcctgcgg ccgcgtcagc
     6061 ggcgtagtag tacagcggcg cgccaccgag cgtcacctgg agcttgccgt cgtcgcgcgt
     6121 gatcgtggcg atcgcgccgg tgacgcccgc gcccgcgtgg gcatcagccg cattcgccac
     6181 gatgagcggc ggccagttgg tcgcgcaccc gcccgagcac gcgctcttgc cggcgctgtc
     6241 cttcgagaag acgtacaggg acttgccgcc ctcgccggcg acgtacgcgc cgagcgtcgg
     6301 gctctggctg atcgcgaggg tgacgccggc catcggtgcg gtggtcgcgg caggcggcgc
     6361 ggccgaaacg ggggcagtgg tgggtccggc gccaggcgct gcgcacgcgg cgacgagggc
     6421 gaggcttgcc gcaaagatcg ccgcgccgcg cccgaggcgc ccctgcgccg acgccgaccc
     6481 ggttcgtgtc cgttccatcg agcaccctcc tccgcgcgcg gcccctcggg ccactaccgc
     6541 acgatacgcc cggcgcgcga tctcggatct cggctagcgc gggcgcaggc cgccgcgtgc
     6601 gacgattctc gcctccgcat cgtcccagcc gggatcgaac gccttgcccg gcgtgtcgag
     6661 gtcgacgacg agcggcgcgt ggtccgacgg aatcggcttg cccttccgcg cctcgcgatc
     6721 gatctcgacc gccacgacgc gatcggcgac cgacgacgtc ggcagcaggt ggtcgatgcg
     6781 cataccgaag ttcttgtgga agttgccggc acggtagtcc caccaggtga accggccccg
     6841 tgcctcgggc tggcgctcgc ggaatgcgtc gtggaggccc caggcgagca ggttccggaa
     6901 cgcctcgcgc tccggctcgg acacgtgcgt gccgccgtgg accgcgcggg cgtcccagac
     6961 gtcgatgtcg gcaggagcga tgttcatgtc gccgccgatc aggagcgcct catccgggtc
     7021 ccgcgccgcg tcgagccagc gccgggcacg tgcgaaccag cgcagcttgc ccgcgtagaa
     7081 cgcggaatcg acgacccggc cgttgggggc gtacaggctg accacgcgca gcggatcgcc
     7141 gccgggctcg gtgacgccga tggacagcat ccgcgcctcg tcggatgggt cgaagtcctc
     7201 ttcctcgagc gagacagagg cgcccgcgcc ggagtcgcgg accgggccat cgccgaagtt
     7261 ggtgatcgga tcgtcgatcg cgatgccgtc acgtaccgcg atggcgacgc cgttccagcg
     7321 accctcgccg tggtggacga gctgatagcc ggccatctgg aaggtcatga gcggtgcgtc
     7381 ggcgtcggag agcttcgtct cctgcatgag caggacgtcg ggcgcggcgc gatccagcca
     7441 ccactcgatc ttctcgagcc gggccttcat cgagttgacg ttccaggtcg cgatgcgcat
     7501 cggctggtat ccgggtcgca ggctcggtcg gcgagcccta gcgcccgcgc acgcccgcga
     7561 tcgtcatcgc gtccatgtca ggcccgcctt cgccgtgctc ctcgaactcg atgccgagtg
     7621 ccgtgaacgc cgcacgcgtc ctgtcggcat cggaggtggt gagcatgagc gcgccttcgg
     7681 tgtcctcggc gacgcccacg atcccggtga tgttgacccc ggccgcgccg agggcagtgg
     7741 cgaccttcgc caacgagccc ggcttgtcct cgagctcgac cgtgaaccag accatcatcg
     7801 gccgccacct cctgtgatgg cgatggtagc gatgcgaggg ccgtcgggcg cccggtaggc
     7861 tgtcgcccga tggccccgcc gaaaccgccc gccggcccga tcgtccagat cttcggcaca
     7921 cccgactcgc agccgacccg cgcctgtctc cggtgcttca aggagcgtcg tctcgaggtc
     7981 cacctcgtcg acatccggcg caaaccgatg gctcccggcg agctgcgtcg cttcgtcgaa
     8041 cgcctcggcg ctcgcgccct cgccgacacc gagggcaagc cctggcgtga cgccggcctc
     8101 ggctacctct ccatgtccga cgccgacctc ggcggtcgcc tcctcgccga ccagcgactc
     8161 ctgaacctgc cgctcgtccg cgcgggcaac gccttcgcgg cggggcgcga cgaagcggcc
     8221 tggaagcgcc tggcggccga cgcgacggcg acggtggcgc cgcgatgagc gggcgtgccg
     8281 cggcccagct cgagtggccg caggtccttg cctggcgact gcgccggcag ctgctggatc
     8341 cgatcgggac cgagccggtc gagggcgtcg tggggcgatt gggggcgatc caggccacga
     8401 acggcgacct ggcgatccgc gtgcggcggt cctcgtcgtc cgcgggcgat ctcgcccagg
     8461 cgctcgccga cggccgcgtc atcagctcgt tcgcgtttcg cggcgcgacg cacctgatga
     8521 ccccggagag cgccggcgcg tatctcgccc tgcggaagtc ggcccggatg ttcgagctgc
     8581 cgagctggca gaggtactac gggctgctgc cgtccgactg ggatgggctg cgggcggtcg
     8641 tgcgcgaggc gctcgccgcc ggtccgaaga cggtcgacga gatcgtcgcg gccatcgggg
     8701 cgagcccgcg gttcgctcac ctcggaccga tcctcgccgg caacccgtgg agcgtgatga
     8761 aggccatcgc gtggcatggc gacatgagct tcgggcaggc tcgaggccgg cagtcggcct
     8821 tccagctcct cgatcgcaat ccgttgtggc ccggcctgtc cgaggtcggc gacgccgcga
     8881 catgggcgat cgaggcgtac ctccgcgcct acggcccggc gacaccgaag cacctgcagt
     8941 actggcttgg cgcgggcctg ggcgcggcgc gcaagctgct gcagggcgcg accgcaacgc
     9001 tccgggaccg gctcgtggag gtccaggtcg aggggcagcc ctgcctcgcg atgaccgatg
     9061 acctcgacga gctcgtcgcc acacgaccca gctccgtcgt gcggctgctg cccgcctacg
     9121 accagtgggt gctcggcccg ggcaccgccg atccgcacgt cgt
//
LOCUS       DATBSW010000295         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_909247, whole genome shotgun sequence.
ACCESSION   DATBSW010000295 DATBSW010000000
VERSION     DATBSW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909247"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..611)
                     /locus_tag="VIF63_08195"
     CDS             complement(105..611)
                     /locus_tag="VIF63_08195"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8239403.1"
                     /translation="MNAPEAAPTVPEAGQMAPDVVLPDETGAVHRLADQKGRWTVLYF
                     YPKDDTPGCNTEACQFRDLDSEYAATDADVWGVSKDSAASHARFREKFDLPFTLLTDP
                     NREVIERYGAWGEKTALGRTYMGIVRSTFLIDPDGRIARAWPKVKADGHPAEVLAALG
                     EERAARAS"
     gene            complement(608..1306)
                     /locus_tag="VIF63_08200"
     CDS             complement(608..1306)
                     /locus_tag="VIF63_08200"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEY8239404.1"
                     /translation="MPLNGALIFADGGVPPRALLDATWPDWAAGVRIHIAADGGARSA
                     AAFGIRLDYWVGDGDSIDDATLDRLALEGVAVQRAAPDKDESDTELALLAAIRHGADA
                     VVILGALGGVRFDHALANIGLLRHPALDGRPVRLYDEVASRISLISSPDDDGIPVTVD
                     FEGRRGDLVSLLPLGLAAEGVRTEGLQFELRDEVLSGGRTRGLSNIRTADVARITVEY
                     GWLLVIETPATVRP"
     gene            complement(1306..1971)
                     /locus_tag="VIF63_08205"
     CDS             complement(1306..1971)
                     /locus_tag="VIF63_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8239405.1"
                     /translation="MDAIVFDWDGTLVDSLPAIFDANHRVLTEYGLPFDEARYRAAYV
                     PDWRLMYQRLGIPDESLDAAGERWLELYRSTEEAGLLPGVADALHRLREAGFVLGLVT
                     AGHRDVVDDQLERYGLGELIRTRVFGSDDIAAKPHPDPLLRVLDELGRSDRIATARYV
                     GDVPDDMRMAKAVGTLGIGIESTIGSRDVLLAAGATEVYPSVAAFVAALLDAPLDPTG
                     GRD"
     gene            2013..2534
                     /locus_tag="VIF63_08210"
     CDS             2013..2534
                     /locus_tag="VIF63_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8239406.1"
                     /translation="MPTTLEHPAVQRVLDLAARKGVTLEVMTFPDSTHTAADAARVVG
                     AEIGQIVKSLVFVAPGDDGELEPIVCLVSGADRVDLARLAAVIVRRDVRRATAKEADE
                     LTGFTIGGIPPFGHTRRTRVIMDPALGRYETVWAAAGLPTAVFPVQPAILRMLADAHV
                     APIAEEPPVERAS"
     gene            2569..>2866
                     /locus_tag="VIF63_08215"
     CDS             2569..>2866
                     /locus_tag="VIF63_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239407.1"
                     /translation="MSAEPRDATISYPGGLRARWRWGGSGQAAAIFALSEVGGQLVDL
                     GPEVSAAPDELCRAELRVDGPAGGWSVRLASTINDEPAAQLWDTAGQLVVKYGFH"
BASE COUNT          389 a         1028 c         1029 g          420 t
ORIGIN      
        1 gcccgaaatc ggcgtcgtcc gggtgggcgg cgatgaccat gaatcgtgcg ggacgccagg
       61 catgggcctc gtgcgcgggg gctccgtcct gttccgtcat cgcatcacga ggcgcgagcg
      121 gcgcgctcct cgcccagcgc ggcgagcacc tcggccggat gtccgtccgc cttgaccttc
      181 ggccaggcgc gggcgatccg cccatcggga tcgatcagga acgtcgagcg gacgatgccc
      241 atgtacgtcc gaccgagggc ggtcttctcg ccccacgccc cgtagcgctc gatgacctcg
      301 cgattcgggt cggtcagcag cgtgaacggc aggtcgaact tctcgcggaa gcgggcgtgg
      361 cttgccgcgc tgtccttgct gaccccccac acgtccgcgt cggtcgcggc gtactccgag
      421 tcgaggtcgc ggaactggca ggcctcggtg ttgcagccgg gcgtgtcgtc cttcgggtag
      481 aagtacagga ccgtccagcg acccttctgg tcggcgagcc ggtggacggc gccggtctcg
      541 tccggcagga ccacgtcggg ggccatctgg ccggcttcgg ggacggttgg tgctgcttcg
      601 ggagcgttca tggccggact gtagcagggg tttcgatcac gagaagccaa ccgtattcga
      661 ccgtgattcg ggccacgtcg gccgttcgaa tgttggacag gccgcgggtc cggccgcccg
      721 acagcacctc gtcccgcagc tcgaattgca gaccttccgt ccggacgccc tcggcggcaa
      781 gcccgagggg caacagcgac accaggtcgc cgcgccggcc ctcgaaatcg accgtcaccg
      841 ggatgccgtc gtcgtccggc gagctgatca gcgagatgcg gctcgcgacc tcgtcgtaga
      901 ggcgcaccgg ccggccgtcg agcgcggggt ggcggagcag cccgatgttc gccagcgcgt
      961 ggtcgaagcg cacgccgccg agtgccccga ggatcaccac tgcatcggcg ccgtgccgga
     1021 tcgcggccag cagggcgagc tcggtgtccg attcgtcctt gtcgggcgca gcgcgctgga
     1081 ccgccacgcc ctcgagggcc aggcgatcca gggtcgcgtc gtcgatcgag tcgccgtcgc
     1141 cgacccagta gtcgagccgg atcccgaacg ccgccgcgct cctcgcccca ccgtccgcgg
     1201 cgatgtggat ccggaccccg gccgcccagt ccggccaggt cgcatcgagg agcgctcgcg
     1261 ggggcacgcc gccgtcggca aagatcaggg cgccgttcag cggcatcagt cgcggccgcc
     1321 agtcgggtcg agcggcgcgt cgaggagcgc cgcgacgaac gcggccacgc tcgggtagac
     1381 ctcggtggca cccgccgcga gcaggacgtc ccgggaaccg atggtggact cgatgccgat
     1441 cccgagcgtg ccgaccgcct tggccatgcg catgtcgtcg ggcacgtcgc cgacgtagcg
     1501 ggcggtcgcg atccgatccg atcggccgag ctcgtcgagc actcgaagga gcggatcggg
     1561 atgcggcttg gctgcgatgt cgtcgctgcc gaacacgcgc gtccggatga gctcgccgag
     1621 cccgtaccgc tcaagctggt cgtcgacgac gtcgcgatgg ccggcggtca cgaggcccag
     1681 cacgaagcct gcctcgcgca gccggtggag cgcgtcggcg accccgggca gcagccccgc
     1741 ttcctcggtc gagcgataca gctcgagcca gcgctcaccc gcggcgtcga gcgattcgtc
     1801 cgggatgccg aggcgctggt acatgagccg ccagtcgggc acatacgcgg cgcgatatcg
     1861 ggcctcgtcg aacggcaggc cgtactccgt gagcacccgg tgattcgcgt cgaagatcgc
     1921 aggcagcgag tcgacgagcg tgccgtccca gtcgaagacg atggcgtcca tgtgccccga
     1981 tcctagcctc cgccgatgct atcctcgccg ccgtgccgac caccctcgag cacccggctg
     2041 tccagcgggt gctggatctc gccgccagga agggcgtgac cctcgaggtc atgacctttc
     2101 ccgactccac gcacaccgca gccgacgccg cgcgcgtcgt gggcgccgag atcggccaga
     2161 tcgtgaagtc cctcgtcttc gttgcgcccg gggatgacgg cgagctcgag ccgatcgtct
     2221 gcctcgtctc cggtgcggat cgtgtcgacc tcgctcgact cgccgcggtg atcgtccggc
     2281 gcgacgtccg ccgcgccacc gccaaggagg ccgacgagct caccggcttc acgatcggcg
     2341 gcatcccgcc gttcggccat acccggcgaa cgcgggtgat catggatcct gccctgggcc
     2401 ggtacgagac ggtgtgggcg gccgcgggcc tgccgaccgc cgtcttcccg gtgcagcccg
     2461 cgatcctgcg catgctcgcc gatgcccacg tcgccccgat cgcggaggag ccgccggtcg
     2521 agcgcgcgtc gtgacctgcc ggcggcgctc gactgagcgc ccgcgctcgt gagcgccgag
     2581 ccgcgcgacg cgacgatcag ctatcccggc gggctgcggg cgcgctggcg ctggggcggc
     2641 tccggccagg cggcggcgat cttcgccctc tcggaggtcg gcggacagct cgtcgatctt
     2701 gggccggagg tttcggccgc gcccgacgag ctctgtcggg ccgagctgcg tgtcgacggg
     2761 ccggccgggg gctggagcgt ccgcctcgcc tcgacgatca acgacgagcc cgccgcgcag
     2821 ctgtgggaca cggccggcca gctggtcgtg aagtacggct tccatg
//
LOCUS       DATBSW010000296         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_64100, whole genome shotgun sequence.
ACCESSION   DATBSW010000296 DATBSW010000000
VERSION     DATBSW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64100"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2248
                     /locus_tag="VIF63_08220"
     CDS             <1..>2248
                     /locus_tag="VIF63_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY8239408.1"
                     /translation="PAKARGKLARLVPGDAKVRFERVKHSQKDLETVQQRINADLGAG
                     RLEGRGILSTAIDTRANAVVVGASRVDDELRDALVRAYGDSVVVTLEGPSQGGDAGCV
                     SRTDCGPAKGGIRIVRTAGGYCTSGPMVRVLGSNALRILTAGHCLALTGGTGTSKTWT
                     HSGSGLGWSEFHTWGNGADADVGLVNPSSYPVGGPRNLLYRSSHTDVVAITSWKPTAE
                     QIQGSLVCRSGAVSGYVCGEITLTNKTKSVDGGSIDHQWVVDFDACPGDSGAPYLVGD
                     VAWGIHSDSTSGCNPDTNEAWYSPMGWVFDTLAQAGHPVELCTNSSCSSGANSWTAQN
                     SLDEAAWNPGLVTLANGRVLRVGGDDGDLVPGATTASPVPSTEIFDPASRTWSDTATP
                     PWLPNHCAGQFAARMADGTVLVGGGTSIEDVEDDPCAAMAYRFDPSTGAGGSWTDVEP
                     MPASIISAGAVLLKDGRVLVTGGTGSDGATSLALAYSPSNDEWTTLTPAPTGAFAPLV
                     LRLLDGRVLVSGGYTLTEIDGTEHDDSAATYLYNPANDTWASTTSVGGHGMAGVVLSD
                     GRVAVAGGDRNASNGSHHALSTTVRLLDPTTGTWTTPAALRTARASFTLVQLPNGQLL
                     AAGGKTPNGTSSTVDVYDWLTNAWYSAANLQGGRAMHGSAVLNDGGVLVAGGATASAE
                     VYRLGDITPPVTATPVARLRTATTLSYPSVPARITWSAQDAGGAGVGTYDVARSTDGG
                     AYSTVGSTW"
BASE COUNT          346 a          798 c          783 g          321 t
ORIGIN      
        1 ccggcaaagg cgcgcggcaa gctggcgcgc ctcgtacctg gagacgccaa ggttcggttc
       61 gagcgggtca agcattccca gaaggacctg gagaccgtcc agcagcgcat caatgcggac
      121 cttggcgccg gtcgcctcga gggccgtgga atcctgagca cggcgatcga cacgcgcgcg
      181 aatgcggtcg tcgtcggtgc gtcgcgggtc gacgacgagc tccgcgatgc gctcgtccgc
      241 gcctacggcg acagcgtggt cgtgaccctc gaaggtccct cgcagggcgg cgatgctggc
      301 tgcgtctcgc ggacggactg cggcccggcc aagggcggca tccggatcgt gcggaccgcc
      361 ggcggctact gcacgagcgg gcccatggtt cgcgtgctcg gcagcaacgc cctgcggatc
      421 ctgactgccg gccattgcct ggcgttgacc ggcggcacgg gcacgagcaa gacgtggacg
      481 cacagcggct cgggcctcgg ctggtccgag ttccacacgt ggggcaacgg cgccgacgcg
      541 gacgtcggcc tggtcaaccc gagctcctat cccgtcggcg ggcctcgcaa cctgctgtat
      601 cggtcgtcgc acacggacgt ggtcgccatc acgtcgtgga agccgacggc cgagcagatc
      661 cagggctcac tcgtctgtcg atcgggcgcg gtcagcggct acgtctgcgg cgagatcacc
      721 ctgacgaaca agaccaagtc ggtcgacggc ggctcgatcg atcaccagtg ggtcgtcgat
      781 ttcgacgcct gcccgggcga cagcggcgcg ccatacctcg tcggtgacgt cgcgtggggc
      841 atccacagcg actcgacgtc cggctgcaac ccggacacga acgaggcctg gtactcgccg
      901 atgggctggg tgttcgacac cctcgcgcag gccggccacc ccgtcgagct gtgcaccaat
      961 tcgagctgca gctccggcgc gaactcgtgg acggcgcaga actcgctcga cgaggcagcc
     1021 tggaaccccg ggctggtcac gctcgccaac ggacgggtcc tccgcgtcgg tggtgacgac
     1081 ggcgacctcg tgcccggcgc gaccaccgcc agtccggtgc cgagcacgga gatctttgat
     1141 ccggcgagcc gcacgtggtc ggatacggcg acgccgccgt ggctgccgaa tcactgcgcc
     1201 ggccagttcg cggctcgcat ggccgacggc accgttctcg tcggcggcgg cacatcgatc
     1261 gaggacgtcg aggacgatcc ctgtgcggcg atggcgtatc gcttcgaccc ttcaaccggc
     1321 gccgggggct cgtggacgga cgtcgagccg atgccggcct cgatcatctc cgccggtgcg
     1381 gtcctgctca aggatggccg cgtgctcgtg acggggggca ccggcagcga cggcgcgacg
     1441 tcgctcgccc tcgcctactc cccgtcgaat gacgagtgga ccacgctgac gccggcgccg
     1501 accggcgcat tcgccccact cgtgctccgc cttctcgacg ggcgggtgct cgtctcgggc
     1561 ggctacaccc tcaccgagat cgacggtacg gagcacgacg acagcgcggc aacgtacctc
     1621 tacaaccccg cgaacgacac ctgggccagc acgacgagcg tcggcggtca tgggatggca
     1681 ggcgtggtgc tctcggatgg gcgagtcgcc gtcgcgggcg gcgatcgaaa cgcgtcgaac
     1741 ggatcccacc atgccctcag cacgaccgtg cggctcctcg atccgacaac cgggacgtgg
     1801 acaacgccgg ccgcgctccg gacagcgcgt gcgagcttca ccctcgtcca gctgccgaac
     1861 gggcagctcc tggccgccgg tggcaagacc ccgaacggga caagcagcac ggtcgatgtc
     1921 tacgactggc tgacgaacgc ctggtattcc gcggcgaacc ttcagggcgg tcgtgccatg
     1981 cacggctctg cggtcctcaa cgacggtggg gtgctggtcg ccggcggcgc caccgcgtcc
     2041 gccgaggttt accggctcgg cgacatcaca ccaccggtga cggcaacgcc cgtcgcgcga
     2101 ctgcggaccg cgacaaccct gtcctatccc agcgtccccg cgaggatcac ctggtccgcc
     2161 caggacgccg gcggtgccgg cgtcggcacc tacgacgtgg ctcgcagcac cgacggcggc
     2221 gcgtattcga ccgtggggtc gacgtgga
//
LOCUS       DATBSW010000297         5635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_945339, whole genome shotgun sequence.
ACCESSION   DATBSW010000297 DATBSW010000000
VERSION     DATBSW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945339"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..499)
                     /gene="rpsG"
                     /locus_tag="VIF63_08225"
     CDS             complement(32..499)
                     /gene="rpsG"
                     /locus_tag="VIF63_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY8239409.1"
                     /translation="MPRRAGIARKTKYEQAPVNRTTVRFHAKLMSDGKRRLAERILRQ
                     ALARAEASARRPGLEVMETALRNATPIIEVKPRRVGGATYQVPVEIRGDRRLSLGVRW
                     LVQAARKRSGKSMSEKLAAEFVDASNGLGAAVKRREDTHKMAEANKAFSHFKW"
     gene            complement(500..931)
                     /gene="rpsL"
                     /locus_tag="VIF63_08230"
     CDS             complement(500..931)
                     /gene="rpsL"
                     /locus_tag="VIF63_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY8239410.1"
                     /translation="MPTINQLVRHGRRPKIAKIKAPAMRKSWNSLRQRTVSIPGSPQK
                     RGVCLQVRTMTPKKPNSALRKIARVRLTNGMEVTAYIPGIGHNLQEHSVVLVRGGKVK
                     DLPSVKYHIIRGTLDAAGVRDRKQGRSKYGAKAAPAIGGKR"
     gene            complement(1190..2581)
                     /locus_tag="VIF63_08235"
     CDS             complement(1190..2581)
                     /locus_tag="VIF63_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8239411.1"
                     /translation="MSVRRRLPALGAAFAALGLLVAGLGPGASSVAGAAYDRFYANFQ
                     GEELMRAINADREAVGLAPFHADPGLAAIARDRSHVCPTKPGLTIRGRARDMADRDYL
                     SHDVQGCQKPGGGAFGTFDLLAAFGVTWSNAGENLSTNNFPSTKVDYATGCSSSGGSC
                     HGSIALPRSVAVAERGWMSSSTHRNLVLSTTFKRFGCAAWNGSENHHVYACYFMAGGD
                     VSSDGTGPAVSQTSGEGDAFAAGSSPKFTATVSDGASLLADGRVLLDGAVIKSWAHDH
                     DGSTESLSVVTSHLTAGHHTLTWWVRDTSMNVRSVDVEFTVKGRATATPKPSAHDKPT
                     PRLETDAPTASHGQAEPSAGADSNSPSASATEPAGQGSPEASDQTAFAPGQATAEPIG
                     DGAGDGAALDRGRTALGQSGGADGGWLLPVLVALALLGTGILAGRRQPGADRRRPGGG
                     IAEPTVTEAGEGP"
     gene            complement(2590..>5635)
                     /locus_tag="VIF63_08240"
     CDS             complement(2590..>5635)
                     /locus_tag="VIF63_08240"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239412.1"
                     /translation="SLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLP
                     KKMALELFKPFVMRQLVEKGFAHNIKSAKRIVERVRPEVWDVLEDVIKDHPVLLNRAP
                     TLHRLGIQAFMPVLVEGSAIQIHPLVCTAFNADFDGDQMAVHVPLSSAAQEEARTMML
                     STANLLSPADGTPVVAPTQDMVLGCYYLTMEKEATRDVAATRIFGSEEDAMYAHELTA
                     RGLASASISHSENEDGSKGTHIAGNLDLHEPIETVVSAWDEAAGALTEQRLRTTVGRI
                     IFNQIVPDRLRFTELTNREMRRTDLKRLVDECYRLLGPADTAELVDGIKDVGFKFATR
                     GGMTIGLWDIVTPPQKVKALKAADDQVTAIDKQFQRGLITDEERYEQVVELWKRTTDD
                     ISGHMMKQLPEDNALRMMTDSGARGNKGNIGQLGAMRGLMADPSGRIIDVPVRSNFRE
                     GMTVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVAQDVITKEDDCGTEEGSWIT
                     SVDTADMTGATWPATDDTTRRNVLDEFRRRLVGRYSAAPMRDPSSKAKTAPLIADRND
                     MITEEVAQKLEDAGIEEVLVRSPLTCEARYGVCRRCYGRNLASGEAVGVGEAVGIIAA
                     QSIGEPGTQLTMRTFHTGGVAGLDITAGLPRVEELFEARVPKGKAEISHIDGTVEILR
                     ADTGTKVKVTSRESYDIALGLPKKAELLAAAGDAIALGQVIARVEGSPDIVAQAEGFL
                     VKAGDGSLVLRAEDIVPREYAIPHNARLLVTESQDVRAGDAITDGPINPQEYLETRGK
                     DAVQRYLVKEVQKVYRSQGVTINDKHIEIIVRQMLRKVRIDQPGDSELLPTELIDRLD
                     FEEVNNRVLAEGGEPATAQTVLLGVTKASLNTSSFLAAASFQETTRVLTEAAINGAKD
                     HLIGLKENVIIGKLIPAGSGAPANLAARKERERRAALEALAGEALDDGTQAYNPFLEG
                     GEPRAAAEEEAVLIGQPVAAADDAEFNPFLTDSSEDKDPDLS"
BASE COUNT          725 a         1883 c         2062 g          965 t
ORIGIN      
        1 acggttcgtg gcatctgcta ctggtcccgg actaccactt gaaatggctg aaggccttgt
       61 tggcctccgc catcttgtgg gtgtcctcgc gccgcttgac cgcggcgccc aggccgttcg
      121 acgcgtcgac gaactcggcg gcgagcttct cgctcatgct cttgccactg cgcttgcggg
      181 cggcctggac gagccagcgc acgccgagcg acaggcggcg atcgccccgg atctcgaccg
      241 ggacctggta ggtcgcgccg ccgacgcggc ggggcttgac ctcgatgatc ggggtggcgt
      301 tgcgcagcgc ggtctccatg acctcgaggc cgggccggcg cgcggacgcc tcggcgcggg
      361 ccagcgcctg gcgcaggatc cgctcggcaa ggcggcgctt gccgtcgctc atgagcttcg
      421 cgtggaagcg gacggtggtg cggttgaccg gggcctgctc gtacttggtc ttgcgggcga
      481 tcccggcgcg gcgcggcatc tagcgcttcc cgccgatggc cggggcggcc ttcgcgccgt
      541 acttgctgcg accctgcttg cggtcgcgca cgccggcggc gtcgagggtg ccgcggatga
      601 tgtggtactt gaccgagggc aggtccttca ccttgccacc gcgcacgagc acgaccgaat
      661 gctcctgcag gttgtggccg atgcccggga tgtaggccgt gacctccatg ccgttggtca
      721 ggcgcacgcg cgcgatcttg cgcagcgcgg agttcggctt cttgggcgtc atcgtgcgga
      781 cctgcaggca gaccccgcgc ttctgcgggg agcccgggat cgagaccgtg cgctggcgca
      841 ggctgttcca gctcttgcgc atcgcgggcg ccttgatctt cgcgatcttg gggcgccggc
      901 catggcgtac gagctggttg atcgtcggca cgaaaacgct actccagttg ttctgacagg
      961 tcgatcgggc cgcggaggcg gtccgcgggc cattccgggc cagggtctgt ggaccccgga
     1021 tgtcccgggt cccatctctc gatggggggc tcggaggggc gtctcggctg gcagccgaag
     1081 ccgtgacctt ccgaagccac gaacgcggag tgtagcaatc ggccgatctc agtgtcaaac
     1141 gtggaccgag cgtcgcggaa cggcctgcgg ggcgtcggcg cgggccgggt cagggcccct
     1201 cgccggcctc cgtgacggtg ggctcggcga tgccgcctcc gggtcgccga cggtcggctc
     1261 caggctgtcg ccggccggca aggatacccg tgccaaggag ggccagcgcc acgaggaccg
     1321 gcagcaacca gccaccatcc gcgccaccgg actggccgag cgcggtccgc ccccggtcga
     1381 gcgcggcgcc gtcgcccgcc ccatccccga tgggctcggc cgtggcctgg cccggggcga
     1441 atgcggtctg atcggatgct tcggggctcc cttgccccgc cggttccgtg gcagacgccg
     1501 acggtgagtt cgaatccgca cctgccgacg gttcggcctg gccgtgcgac gccgtcggcg
     1561 cgtcggtctc gagccgcggc gtcggcttgt cgtgggctga cggcttgggc gtcgccgtgg
     1621 cgcggccctt caccgtgaac tcgacgtcaa cggagcgcac gttcatcgac gtgtcccgga
     1681 cccaccaggt gagggtgtgg tggccggcgg tcaggtgcga cgtgacgacg gacaggctct
     1741 cggtcgaccc gtcgtggtcg tgtgcccagg acttgatgac ggcgccatcg aggagcacgc
     1801 ggccgtcggc gagcagcgat gcaccgtcgc tgaccgtggc ggtgaacttc gggctcgagc
     1861 ccgccgcgaa ggcgtcgccc tcgccgctcg tctgcgacac ggccggcccg gtgccatccg
     1921 acgagacgtc gccgccggcc atgaagtagc acgcgtagac gtggtgattc tcactgccat
     1981 tccacgcggc gcagccgaag cgcttgaacg tcgtcgacag aacgaggttg cgatgtgtcg
     2041 agctgctcat ccagccgcgc tcggcgacgg cgacgctgcg gggcagggcg atcgaaccgt
     2101 ggcagctgcc gccgctcgac gagcaaccgg tggcgtagtc gaccttcgtc gacgggaagt
     2161 tgttggtcga gaggttctcg ccggcattcg accaggtcac gccgaacgcg gcgagcaggt
     2221 cgaacgtccc gaacgcaccg ccgcccggct tctggcagcc ctgcacgtcg tgcgacaggt
     2281 agtcgcgatc ggccatgtcg cgcgcgcggc cccggatcgt cagtcccggt ttcgtgggac
     2341 acacgtgcga ccgatcgcgg gcgatggcgg ccagcccggg atccgcatgg aacggcgcga
     2401 gcccgaccgc ttcgcgatcg gcgttgatcg cgcgcatgag ctcctcgccc tggaagttcg
     2461 cgtagaagcg gtcgtaggcg gcgcccgcca ccgacgatgc cccgggcccg aggccggcga
     2521 ccagcaggcc cagcgcggcg aaggccgcgc cgagcgcggg caatcgccgc cggacggaca
     2581 cgggcgggat caggagaggt cggggtcctt gtcttccgac gaatccgtca ggaaggggtt
     2641 gaactccgcg tcgtcggctg ccgcgaccgg ctggccgatg agcactgcct cttcctccgc
     2701 ggcggcccga ggctcccctc cctcgaggaa ggggttgtac gcctgggtgc cgtcgtcgag
     2761 cgcctcgccg gccagggcct cgagggccgc tcgccgctcc cgctccttgc gcgcggcgag
     2821 gttcgcgggt gcgccggagc cggccgggat gagcttgccg atgatgacgt tctccttgag
     2881 cccgatcagg tggtccttgg cgccgttgat ggcagcctcg gtgagcaccc gggtcgtctc
     2941 ctggaaggac gccgcggcaa ggaagctcga ggtgttcagc gacgccttgg tgacgccgag
     3001 gagcacggtc tgggccgtcg ccggctcgcc gccttcggcg aggacgcggt tgttgacctc
     3061 ttcgaagtcc agtcgatcga tcagctcggt cggcagcagc tcgctgtcac caggctggtc
     3121 gatgcggacc ttgcgcagca tctggcggac gatgatctcg atgtgcttgt cgttgatcgt
     3181 cacgccctgg ctgcggtata ccttctggac ttccttgacg aggtagcgct ggacggcgtc
     3241 cttgccccgc gtctcgaggt actcctgcgg gttgatcggc ccgtcggtga tcgcatcgcc
     3301 ggcccggacg tcctggctct cggtgacgag gagccgggcg ttgtgcggga tcgcgtactc
     3361 gcgggggacg atgtcctccg cgcgcagcac gaggctgcca tcgccggcct tgacgaggaa
     3421 gccctcggcc tgcgcgacga tgtccggtga gccctcgacg cgggcgatga cctggcccag
     3481 cgcgatcgcg tcgcctgcgg cggcgaggag ctcggccttc ttgggcagcc cgagcgcgat
     3541 gtcgtaggac tcgcggctcg tgaccttgac cttcgtgccg gtgtcggcgc gcaggatctc
     3601 gacggtgccg tcgatgtggc tgatctcggc cttgcccttg ggcacgcggg cctcgaacag
     3661 ctcctcgacg cgcggcaggc cggccgtgat gtccaggccc gcgacgccgc ccgtatggaa
     3721 cgtgcgcatc gtgagctggg tgcccggctc gccgatggac tgcgcggcga tgatgcccac
     3781 cgcctcgccg acgcccacgg cctcgccgga ggcgaggttg cggccgtagc agcgacggca
     3841 gacgccgtag cgcgcctcgc aggtgagcgg tgagcggacg aggacctcct cgatccccgc
     3901 gtcctccagc ttctgcgcga cttcctcggt gatcatgtcg ttgcggtccg cgatgagcgg
     3961 cgcggtcttg gccttcgagc tcgggtcgcg catcggcgcg gccgagtagc ggccgacgag
     4021 ccggcggcgg aattcgtcga ggacgttccg tcgcgtcgtg tcatcggtcg cgggccaggt
     4081 ggcgccggtc atgtcggccg tatcgaccga cgtgatccag ctgccctcct cggtgccgca
     4141 gtcgtcttcc ttggtgatga cgtcctgcgc cacgtcgacg aggcgccggg tcaggtagcc
     4201 cgagtccgcc gttcgaaggg cggtgtcggc gaggcccttg cgggcgccgt gggtggagat
     4261 gaagtactcg aggaccgtca tgccctcgcg gaagttgctc cgcaccggca cgtcgatgat
     4321 ccggcccgac gggtcggcca tcaggccgcg catggcgccg agctggccga tgttgccctt
     4381 gtttccgcgg gcgcccgagt cggtcatcat ccgaagcgcg ttgtcctcgg ggagctgctt
     4441 catcatgtgg ccggagatgt cgtcggtcgt gcgcttccac agctcgacga cctgctcgta
     4501 gcgctcctca tcggtgatga ggccgcgctg gaactgcttg tcgatcgcag tgacctggtc
     4561 gtcggcggcc ttgagcgcct tgaccttctg gggcggcgtg acgatgtccc acaggccgat
     4621 cgtcatcccg ccgcgggtgg cgaacttgaa gccgacgtcc ttgatgccgt cgacgagctc
     4681 ggccgtgtcg gccgggccga gcaggcggta gcactcgtcc acgaggcgct tgaggtcggt
     4741 gcgccgcatc tcacggttgg tgagctcggt gaagcgcagc cggtccggca cgatctggtt
     4801 gaagatgatc cggccgacgg tcgtccgcag gcgctgctcg gtgagcgcgc cggcggcctc
     4861 gtcccacgcc gagacgacgg tctcgatggg ctcgtggagg tcgaggttgc ccgcgatgtg
     4921 cgtgcccttg gaaccgtcct cgttctcgct gtggctgatg ctcgcgctcg cgagcccacg
     4981 ggccgtcagc tcgtgggcgt acatcgcgtc ctcttcggac ccgaagatgc gggtcgcggc
     5041 gacgtcgcga gtcgcttcct tctccatcgt caggtagtag cagccgagga ccatgtcctg
     5101 ggtcggcgcc acgaccggcg tgccgtcggc gggcgacagc aggtttgcgg tcgacagcat
     5161 catcgtccgc gcttcttcct gggcggccga ggacagcggc acgtggaccg ccatctggtc
     5221 gccgtcgaag tcggcgttga acgcggtgca gacgagcggg tggatctgga tggccgagcc
     5281 ctcgacgagc acgggcatga atgcctggat gccgaggcgg tgcagggtcg gggcgcggtt
     5341 gagcaggacc gggtggtcct tgatcacgtc ctcgaggacg tcccagacct cgggccggac
     5401 gcgctcgacg atgcgcttgg cgctcttgat gttgtgggcg aagcccttct cgacgagctg
     5461 gcgcatcacg aacggcttga acagctcgag ggccatcttc ttgggcaagc cgcactggtg
     5521 gagcttgagc tccgggccga cgacgatgac cgagcggccg gagtagtcga cgcgcttccc
     5581 gagcaggttc tggcggaacc ggccctgctt gcccttgagc atgtcgctca ggctc
//
LOCUS       DATBSW010000298         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_169052, whole genome shotgun sequence.
ACCESSION   DATBSW010000298 DATBSW010000000
VERSION     DATBSW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169052"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="VIF63_08245"
     CDS             complement(<1..2167)
                     /locus_tag="VIF63_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239413.1"
                     /translation="MSFRSRARRTPGHGPRVLKGLLALSAALALSLPMPSTIGLVPPV
                     VDAKVDHAPVVGGWVTNKFPVGMPTCTGVGYPGATYYDRLDCGFGWVTVSETTAASVV
                     KIATVTGDGVVRGTQTATYRAADVAWQFTIKPDATWPAGPFTLRVTEVDGVSGNYGET
                     SLLFNQLGATIGAAAGSYSPGSAIPVTGTTFEIDQVPPLAGEMNTNVGATFYLRATWP
                     DGTVRGPYGPFTANPGPLNKGTYTASLPGGATTGLTADASTNFKATVAIEVVNATFTD
                     AATGAWGASRAGAGSVTFTLPPTQLTIENSFVSAVGWVKPGDSYPFRVLVKNYTATAR
                     TSAAVTIPAPSGTAFTTATAVSGSGTCTVNAGGTGLTWTIGPVAAATSGVPTIKTCVV
                     EAKARTLAQDAQIVWKNLSSTATLTYTGGPAGNTSTSHGPKVIPPSATFDTARYGDRP
                     FPVVPVDWFDRKHSASHSGESLSDKINSPSIAGSTFNLYQEMSYGQLFPNGTVPSAAL
                     ATADWTYGPGFNFTTPAPGGTCTGTTFKDVKGTPIYGERIVNGWYQMPGDTGYYGSDA
                     DGSALLGPESGVGALQMIDNGCGPTGKAVFDAAQIADPEIDYSDYDTDKDGVVDFFMM
                     VFVGLGGNGYSQTDLNGPYDNIWPHSSSLEFTYTDPDTGLKGYISDDQLKDLQSRPLY
                     YTDDNYVTMTTTVTPFPVYVRVGPYNVNPESAIDKASVIS"
BASE COUNT          333 a          714 c          897 g          393 t
ORIGIN      
        1 gcgagatcac gctggccttg tcgatggccg actcggggtt gacgttgtac ggaccgaccc
       61 gcacgtagac ggggaacggc gtgaccgtgg tggtcatggt cacgtagttg tcgtcggtgt
      121 agtacagcgg gcggctctgg aggtccttca gctggtcgtc ggagatgtag cccttcaggc
      181 ccgtgtccgg gtcggtgtag gtgaactcca ggctcgacga gtgcggccag atgttgtcgt
      241 acgggccgtt caggtcggtc tgcgagtagc cgttcccgcc gaggccgacg aacaccatca
      301 tgaagaagtc gacgaccccg tccttgtcgg tgtcgtagtc gctgtaatcg atctccgggt
      361 cggcgatctg ggcggcgtcg aacacggcct tgccggtcgg gccgcagccg ttgtcgatca
      421 tctggagcgc cccgacaccg gactccgggc cgaggagcgc gctgccgtcg gcgtccgagc
      481 cgtagtagcc cgtgtcgccc ggcatctggt accagccatt gacgatccgc tcgccgtaga
      541 tcggcgtgcc cttgacgtcc ttgaacgtcg tgccggtgca cgtcccgccg ggggcgggcg
      601 tggtgaagtt gaagccgggg ccgtaggtcc agtccgcggt cgcgagcgcg gccgacggca
      661 cggtgccgtt cgggaagagc tgcccgtacg acatctcctg gtagaggttg aacgtggagc
      721 cggcgatcga gggtgagttg atcttgtcgc tcagcgactc acccgaatgg ctcgccgaat
      781 gcttgcggtc gaaccagtcg acgggcacga ccgggaacgg ccgatcgccg tagcgggccg
      841 tatcgaacgt cgcgctgggc gggatgacct tcgggccgtg gctcgtcgag gtgttgccgg
      901 caggtccgcc ggtgtaggtg agggtggcgg tgctcgaaag gttcttccag acgatctggg
      961 cgtcctgggc gagggtcctc gccttcgcct cgacgacgca ggtcttgatg gtcgggacgc
     1021 cgctcgtggc agcggccacg ggaccgatgg tccatgtgag cccggtgccg ccggcgttca
     1081 cggtgcaggt cccggagccg ctgaccgcgg ttgccgtcgt gaacgcggtc ccgctgggcg
     1141 cgggaatcgt gaccgcggcg ctggtgcgtg cggtcgcggt gtagttcttg acgaggaccc
     1201 ggaacgggta ggaatcaccg ggcttcaccc agcccacggc cgagacgaag ctgttctcga
     1261 tggtcagctg ggtcggcggc agggtgaacg tgaccgagcc ggcgccggcg cgggatgcgc
     1321 cccaggcgcc ggtcgctgcg tcggtgaagg tggcgttcac gacctcgatc gcgacggtcg
     1381 ccttgaagtt cgtcgacgcg tcggcggtga ggccggtcgt ggccccgccc gggagggaag
     1441 cggtgtatgt gcccttgttc agcgggccag gattggcggt gaacggcccg tacggcccgc
     1501 ggaccgtgcc gtcgggccag gtggcgcgca ggtagaacgt cgcgccgaca ttcgtgttca
     1561 tctcgccggc gagcggcggg acctggtcga tctcgaaggt cgtgccggtg acggggatcg
     1621 cgctgccggg cgagtaggag cccgcggcgg cgccgatggt cgcaccgagc tggttgaaca
     1681 gcagggaggt ctcgccgtag ttgccgctga cgccgtcgac ctcggtgacg cgcagcgtga
     1741 aggggccggc cggccaggtg gcgtcgggct tgatggtgaa ctgccacgcg acgtcggcgg
     1801 cgcggtaggt ggccgtctgc gtgccgcgga cgacaccgtc gccggtgacc gtggcgatct
     1861 tgaccacgga cgcggccgtc gtctcgctga ccgtgaccca gccgaagccg cagtcgagcc
     1921 ggtcgtagta ggtcgcgccg gggtagccga cgccggtgca ggtcggcatg cccacgggga
     1981 acttgttggt gacccagccg ccgacgacgg gggcgtgatc gaccttggca tcgacgaccg
     2041 gcgggacgag cccgatggtc gagggcatgg gcagcgacag cgcgagcgcg gcgctcagcg
     2101 cgagcagtcc cttgagaacg cgaggtccgt ggccgggtgt ccggcgggca cgacttcgga
     2161 acgacacgtg cgtctcctcc tccgaagtgc gtcggccgct cgatcagcgc gccgtgcgca
     2221 cgatgccacc acgcagagtg gttacttggc gcggagacta gacaccacta agaagggtgt
     2281 caacggatgg gacgcggctg tcacgcgttt gcaatgtcgc ccgcgccggg gtcagat
//
LOCUS       DATBSW010000299         6335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_205070, whole genome shotgun sequence.
ACCESSION   DATBSW010000299 DATBSW010000000
VERSION     DATBSW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205070"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VIF63_08250"
     CDS             <1..465
                     /locus_tag="VIF63_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEY8239414.1"
                     /translation="AIHEADIVVLGPGSLDTSVLPVLLIPAIEDAVLAAPGMRIYACN
                     VAGQPGETEGYDLADHVEALLRHTQPDLIDLVLANDRFGDDTEGDDAWPADAVRPRWP
                     PAVEPVPHLATDAVASADDPHHHDPERLAAAILRVYERDGSTMRRERVARTA"
     gene            467..1240
                     /gene="whiA"
                     /locus_tag="VIF63_08255"
     CDS             467..1240
                     /gene="whiA"
                     /locus_tag="VIF63_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEY8239415.1"
                     /translation="MAGSERDLVLALRAELAAIDPSRPCDRDAERAGLEPAGRRRDPA
                     LARLSVRLARRDGPPGAAAETGTIAFAWPARPDHCRMAWLRGRFLSQGSLSFANGRTH
                     LELVVAPDEAPELARHLADAGIPPSWRIRRGRGVVTWKSAEMVHVVLRRIGAGPSLLE
                     LEARQVTRTLRGELNRVLNAESANLTRTVAAAGRQLDWIATLDADGRLAREPATVRAV
                     AGARRETPEATLGELADRLAMHRSAVQRALVRIERLARA"
     gene            1249..2034
                     /gene="tpiA"
                     /locus_tag="VIF63_08260"
     CDS             1249..2034
                     /gene="tpiA"
                     /locus_tag="VIF63_08260"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY8239416.1"
                     /translation="MRPVIVAGNWKMHTRPADAGELAATIARRSNEPDVVRVLCPPFV
                     CLAAVRDALAAEPAGRDVAVGAQNVHHEAAGAYTGETSAGMLEGLATWVIVGHSERRR
                     DAGETDALISRKVLRARDAGLRVILCVGEQLADRESGAAEEVVQRQVRGSLEGVASPA
                     PWLTIAYEPVWAIGTGRNAQGSDAAAMAGVIRATLAELGFADGGESIPVLYGGSVSSS
                     NIDEFMTEPSIDGALVGGASLKPDEMAAIVARAGLTARARAQR"
     gene            2031..3581
                     /gene="gpmI"
                     /locus_tag="VIF63_08265"
     CDS             2031..3581
                     /gene="gpmI"
                     /locus_tag="VIF63_08265"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY8239417.1"
                     /translation="MTPPRPIVLVIIDGFGIGTDPAADAIAAARMPVWRSLLARWPHS
                     QLEASAGAVGLPPGQMGNSEVGHLNIGAGRPVLQDLPRIDAAIADGSFFERPALLAAT
                     RQARDGAGTLHVISLIGPGGVHANDRHLVALAELAARQGVDRVRVQALLDGRDTPPRS
                     ALGFVQDLEPRLAAAHPDARIASVGGRYFAMDRDKRWDRTEKGYDAIVHGEGLHAASA
                     SAAIEAGYARGENDEFVTPTVIDGAGGAVHGGEPIVVANFRADRMRQLTHALADTTFG
                     AFDRTAPSGRRAPGMDVTTMTEYEADLPVAVAFAPEVVACLAGAAADAGWHQLHVAET
                     EKYAHVTYFFNGGREAAFDGEERVLVPSPKVATYELQPEMSAAGVTDALIAGIESDRF
                     DLLVANFANPDMVGHTGDWAATLAAVETIDGCLGRIVDALAPLETSGALLAITADHGN
                     ADAMRTPDGSIVTAHSLNPVPLLLAGAAVDGRSLANGVLADVAPTLIELAGLTPWIGM
                     TGRSLLRD"
     gene            3640..3876
                     /gene="secG"
                     /locus_tag="VIF63_08270"
     CDS             3640..3876
                     /gene="secG"
                     /locus_tag="VIF63_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY8239418.1"
                     /translation="MHPVLAAGQLIVAVAVTLAILLQSRGSGLGGTFGGDSAVYRSRR
                     GIERRLWQFTVVLIVLFTMFSLAGYILSPSVAAS"
     gene            3916..5652
                     /locus_tag="VIF63_08275"
     CDS             3916..5652
                     /locus_tag="VIF63_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8239419.1"
                     /translation="MKLRDRALVGILSVALIALSIAALAPSLQSSDGSDEPTGSIPNT
                     RHYVEGVLGRASNASPFGARSAADRALVALLFRGLVRLGPHSSIVGDLAARWEVDADG
                     RTWTFHLRDGLFWEDGEPLTADDVAFTVDVLSDPSYIGPGGESWQDVVATVVDPMTVQ
                     LHLSTPLGGFLQAATQPIAPAHLLAEVPPDQIADDPFGAAPVGSGPFRIVYMLDSVAM
                     LEAATPIEALPEDPGRPNFFTPRPTDSSASARPTPKSDVAVPYLSKFELRYYDDIDSL
                     RADWTKGDLDAAYGLQPADAGALAAGVGARLVQYPGTTMLGAALNLRGGEPAFKDPAV
                     RRALLQAIDRDTLVATALDGLGTRADSLIPPTSPMFDAAASAAVKFDPAAARKALTTA
                     GWKPSDTGWIPKGAKEPLVIELLSPEEPANPVAYAAAAALVDAWQDIGLAVRQVPLPA
                     AELIGERLVQGDFSVAVLPFAIGLDPDVYPLLAASQTRTGGSNVIGLQDPDLDKLLVA
                     ARTPTDPEQRLAAYKALQARLADRTYMLPLAFRDDYVVFRDTVVGPESRPIGSSGDRY
                     WDVLTWRLADGS"
     gene            5681..5767
                     /locus_tag="VIF63_08280"
     tRNA            5681..5767
                     /locus_tag="VIF63_08280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5715..5717,aa:Leu,seq:cag)
     gene            5822..5906
                     /locus_tag="VIF63_08285"
     tRNA            5822..5906
                     /locus_tag="VIF63_08285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5856..5858,aa:Leu,seq:gag)
BASE COUNT          844 a         2343 c         2219 g          929 t
ORIGIN      
        1 gcgatccacg aggcggacat cgtggtcctc gggccgggca gcctggacac gagcgtgctg
       61 ccggtccttt tgatcccggc gatcgaggac gcggtcctgg ccgcacccgg catgcgcatc
      121 tacgcctgca acgtcgcggg ccagccgggc gagaccgagg gctacgacct cgccgaccac
      181 gtcgaagcgc tgctccgaca cacccagccg gacctgatcg atctcgtcct ggcgaacgat
      241 cggttcggcg acgacacgga gggcgacgat gcgtggccgg cagacgcggt acggccgcgc
      301 tggccgccgg cggtcgagcc cgtgccccac ctcgcgaccg atgccgtcgc gagcgccgac
      361 gatccccacc accatgaccc ggaacggctc gccgccgcga tcctgcgcgt gtacgagcgc
      421 gacggctcga cgatgcgacg cgaacgagtc gcgcgcaccg cctgacgtgg ccggctcgga
      481 gcgggatctc gtcctcgcac tccgcgcgga gctggccgcg atcgatccat cgcggccgtg
      541 tgatcgcgac gcggagcggg cggggctcga gccggccggc cggcgtcgcg acccggccct
      601 tgcccgcctc tccgtccggc tcgcccgccg cgatgggccg ccgggcgcgg ccgccgagac
      661 gggcacgatc gccttcgctt ggccggcccg gcccgaccac tgccggatgg cctggctgcg
      721 tggccgcttc ctgtcgcagg gctcgctgag cttcgccaac ggccggaccc acctcgagct
      781 cgtcgtggcg cccgacgaag ccccggagct ggcccggcac ctggccgacg ccggcatccc
      841 gccgagctgg cgcatccgcc gcggtcgggg cgtggtcacg tggaagagcg ccgagatggt
      901 ccacgtcgtg ctgcgccgga tcggcgccgg gccgagcctc ctcgagctgg aggcgcgcca
      961 ggtgacccgg acgctgcgcg gtgagctcaa tcgcgtgctg aacgccgagt cggccaacct
     1021 cacccgcacg gtcgcggcgg ccggccggca gctggactgg atcgcgaccc tcgacgccga
     1081 cgggcgcctc gcccgcgaac ccgcgacggt tcgggcggtc gccggcgccc gccgcgaaac
     1141 gcccgaggcg acgctcggtg agctcgccga tcggttggcc atgcaccgtt ctgcggtgca
     1201 gcgcgccctg gtgcgcatcg agcggctggc acgggcatga ttcgcctgat gcgaccggtg
     1261 atcgttgcgg gcaactggaa gatgcatacg aggccggccg acgccggtga gctggcggcg
     1321 accattgccc ggcgctcgaa cgagccggac gtggtccggg tgctgtgccc gccgttcgtg
     1381 tgcctcgcgg ccgttcgcga cgccctcgcc gcggagcccg ccggccggga cgtggcggtc
     1441 ggggcgcaga acgtgcatca cgaggccgcc ggtgcgtaca cgggcgagac cagtgccggg
     1501 atgctcgaag ggctcgccac ctgggtgatc gtcgggcaca gcgagcgccg gcgtgacgcg
     1561 ggcgagaccg acgcgctgat cagtcgcaag gtgctccggg cgcgtgacgc cgggctgcgc
     1621 gtgatcctgt gcgtgggcga gcagctcgcg gatcgcgagt ccggcgccgc cgaagaggtc
     1681 gtccagcgcc aggtccgcgg cagcctcgag ggcgtggcga gtccggcgcc gtggctgacg
     1741 atcgcctacg agcccgtctg ggcgatcggc acggggcgga atgcccaggg ctccgatgca
     1801 gcggcaatgg ccggcgtgat ccgggcgacc ctggccgagc tgggcttcgc ggatggcggc
     1861 gaatcaatcc ccgtgctcta cggcggcagt gtctcgtcgt cgaacatcga cgagttcatg
     1921 accgagccct cgatcgacgg cgcgcttgtc ggcggcgcct cgctcaagcc cgacgagatg
     1981 gcggcaatcg tcgcgcgcgc cggcctcacg gcgcgcgctc gcgcccagcg gtgacgcccc
     2041 cgcggccgat cgtcctcgtg atcatcgacg gcttcgggat cgggaccgac ccggcggccg
     2101 acgcgatcgc cgccgcgcgt atgcccgtct ggcgctcgct gctggcgcgc tggccccact
     2161 cgcagctcga ggcttccgcc ggagcggtcg gcctgccgcc cggacagatg ggcaactccg
     2221 aggtcgggca cctgaacatc ggcgccggcc ggcccgtgct ccaggacctg ccccggatcg
     2281 acgcggcgat cgccgatggc tcgttcttcg agcggccggc gctgctggcc gcgacccggc
     2341 aggcacgcga cggcgccggc acgctccacg tcatcagcct gatcgggccc ggcggcgtcc
     2401 atgccaacga tcgacacctg gtcgcgctgg cggagctcgc cgcacgacag ggtgtcgacc
     2461 gagttcgtgt ccaggcgctg ctcgacggtc gggatacgcc cccgaggtcg gccctcggtt
     2521 tcgtgcagga tctcgagccg cgcctcgcgg ccgcgcaccc cgatgcccgg atcgcatctg
     2581 tcggcggccg gtacttcgca atggaccgcg acaagcgctg ggaccgcacc gagaagggct
     2641 acgacgcgat cgtccacggc gagggactgc acgccgcgtc ggcgagcgca gcgatcgaag
     2701 ccggatacgc ccgcggcgag aacgacgagt tcgtcacgcc caccgtcatc gatggcgccg
     2761 gcggcgccgt tcacggcggc gagccgatcg tcgtcgccaa cttccgagcc gatcgcatgc
     2821 gccagctgac ccacgcgctg gccgacacga ccttcggcgc cttcgaccgg acggcgccat
     2881 cgggccgtcg tgcgccaggc atggacgtca ccacgatgac cgagtacgag gcggacctgc
     2941 cggtcgccgt cgcgttcgcg ccggaggtcg tggcctgcct cgccggcgcc gcggccgacg
     3001 ccggctggca ccagctgcac gtcgccgaga ccgagaagta cgcccacgtg acctacttct
     3061 tcaacggcgg ccgcgaagcc gcgttcgacg gcgaggagcg tgtcctcgtg cccagcccga
     3121 aggtcgcgac ctacgagctc cagcccgaga tgagcgcggc cggcgtcacc gacgccctga
     3181 tcgccgggat cgagtccgac cgattcgacc tgctcgtggc gaacttcgcg aacccggaca
     3241 tggtcggcca caccggcgat tgggctgcca ccctggccgc cgtcgagacg atcgacggct
     3301 gcctcgggcg tatcgtcgac gcgctggcgc ccctcgagac ctctggcgcg ctcctcgcga
     3361 tcactgcgga ccacggcaat gccgacgcca tgcggacgcc ggacgggagc atcgtgacgg
     3421 cccactcgct gaacccggtg ccgctgctcc tcgccggcgc ggcggtcgat ggccgctcgc
     3481 tggcgaacgg cgtcctcgcg gacgtggcac cgacgctcat cgagctcgcc ggcctgaccc
     3541 cgtggatcgg catgactggc cggtcgctcc tgcgcgactg agcgtgattc gcgactgacc
     3601 ctgctaccat cccgcggtca tcgaggaggt tcccaaaccg tgcatcccgt cctggccgcg
     3661 ggtcagctca tcgtcgccgt cgcggtcacg ctcgcgatcc tgctccagtc ccgcgggtcg
     3721 ggcctcggcg ggacgttcgg cggtgactcc gccgtgtacc gcagccgccg cggcatcgag
     3781 cgccggctgt ggcagttcac ggtcgtcctg atcgtcctgt tcaccatgtt ctccctcgcc
     3841 ggatacatcc tctcgccgtc cgtcgcggcc agctgacacg gtcgggcgct tgcgccccag
     3901 ggtctcgatt ccaccatgaa gcttcgcgac cgcgccctcg tcggcatcct gagcgtcgcc
     3961 ctgatcgcgc tgtcgattgc ggcactcgcg ccatccctgc agtcctcgga cggctccgac
     4021 gagccgaccg ggtcgattcc caacacgcgg cactacgtcg agggcgtcct cggccgggcc
     4081 tcgaacgcga gcccgttcgg cgcccgctcg gccgcggatc gcgcgctcgt ggcgctgctg
     4141 ttccggggcc tcgtccgcct cggcccgcac agctcgatcg tcggcgacct cgcggcgcgc
     4201 tgggaggtcg atgccgacgg gcgaacgtgg acgttccacc tgcgtgacgg cctcttctgg
     4261 gaggacggcg agccgctgac ggccgacgac gtggccttca ccgtcgacgt cctgagcgac
     4321 ccgtcctaca tcggtccggg cggggagtcg tggcaggacg tcgtggcgac ggtcgtcgac
     4381 ccgatgaccg tgcagctgca cctgagcacg ccgctcggcg ggttcctcca ggccgcgacg
     4441 caaccgatcg caccggcgca cctgctcgcg gaggtcccac ccgaccagat cgcagatgac
     4501 ccgttcggtg ccgccccggt cggctcgggc ccgttccgca tcgtgtacat gctggactcc
     4561 gtggccatgc tcgaagccgc gacgccgatc gaggcgctcc cggaggaccc cgggcggccg
     4621 aacttcttca cgccgcgccc gaccgactca tcggcctcgg cgcgcccaac gccgaagtcg
     4681 gatgtcgccg tcccgtacct ctcgaagttc gagctgcgct actacgacga catcgactcg
     4741 ttgcgcgccg actggacgaa gggcgacctc gacgcggcgt acggcctcca gcccgccgac
     4801 gcgggggcgc tggcggcggg cgtcggcgca cggctcgtgc agtacccggg cacgaccatg
     4861 ctcggcgctg ccctcaacct gcggggcggt gagcccgcgt tcaaggaccc ggcggtccga
     4921 cgcgcgctcc tgcaggcgat cgatcgcgac acgctcgtcg cgacggccct cgacggcctc
     4981 gggacccgcg ccgattcgct gatcccgccg acgtcgccga tgttcgacgc cgcggcgagc
     5041 gcggccgtga agttcgaccc cgcagcggcg cggaaggccc tcaccaccgc cggctggaag
     5101 ccgtcggaca ccggctggat cccgaagggc gccaaggagc ccctcgtcat cgagctgctc
     5161 agccccgagg agcccgcgaa ccccgtcgcg tacgccgcgg cagcggccct ggtcgatgcc
     5221 tggcaggaca tcgggctggc ggtccggcag gtgccgctgc ccgcggccga attgattggt
     5281 gagcgactcg ttcagggcga tttctcggtg gccgtgctgc cgttcgcgat cgggctcgac
     5341 cccgacgtct acccgctgct cgccgcgtcg cagacgcgaa ccggtggatc gaacgtcatc
     5401 gggctccagg accccgacct cgacaagctc ctcgtcgccg cccgaacccc gaccgacccg
     5461 gagcagcggc tggccgccta caaggccctc caggcacgtc ttgccgaccg cacgtacatg
     5521 ctgccgctgg ccttccgcga cgactatgtg gtgttccggg acacggtcgt cgggcccgaa
     5581 tcgaggccca tcgggagctc cggggaccgc tattgggacg tgctaacatg gcggctcgcc
     5641 gacggcagct aaggcctttt cgggtcgccg tggatctcac gccgaggtgg cgaaattggc
     5701 agacgcgcta gcttcaggag ctagtgcccg caagggcgta tgggttcaag tcccttcctc
     5761 ggtaccaatt tcaaccctcg accctctctg atccacgaac ctagccggca gcgaacctga
     5821 tgcccaggtg gcggaattgg tatacgcgta cgtttgaggg gcgtatgagg caactcatcc
     5881 gggttcaagt cccggcctgg gcaccactcc tcccttgatc caccgcggct gacgccgccg
     5941 gccgccgccc gcgcctccct gcgcccctcg cgtcgcttcg ctcgctcggt tctgatggtc
     6001 gggcggtcgg cggcctatgg cgccatccgc ggatcggcgg tcgtcgagag atcttccggg
     6061 cgccgatggt cgcgtttctg ccggattcca cgcccggtgg cgttcggcga cgtcgcaggg
     6121 gctattcccg gtcaacgcca ctgattttca tcccggcgtg agcgtggctc gggatgaacc
     6181 gccttccgga gaggctcgaa atcgggggtc gagcctgccg agtgctaccc gccgtggaat
     6241 ttggcaggtt tggcacgggg cccgcgggct gtaggcttcg tgcgggcggt tagctcagtt
     6301 ggttagagcg cctcgcttac accgagggtg tcggg
//
LOCUS       DATBSW010000300         7496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1192754, whole genome shotgun sequence.
ACCESSION   DATBSW010000300 DATBSW010000000
VERSION     DATBSW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192754"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VIF63_08290"
     CDS             complement(<1..843)
                     /locus_tag="VIF63_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239420.1"
                     /translation="MRPTTPRWVTLLAGAIWLVTLAMVLGILWVAYGTTYVRDMSAFV
                     TAGASPGADNIGILALAMTAFCAIAIGYATVGALLAGRPGAGRIGWVLLAGGALFVLI
                     PFGYMVGGILTFANPDSSVNQLLLLIGPVALGPGVVAILPALAIMFPDGRLPSLRWSL
                     PVAVAATITGFGTFVQIITPTVVAGPIGDRNPIGIQGLPPFLASLGYVGVAGGIVSMT
                     ALGVAAVIVRYRRGTPVERQQARWFVVAMGRAALPLMLSVLPGLGGPVTLLRASFGVL
                     LVPIS"
     gene            complement(895..971)
                     /locus_tag="VIF63_08295"
     tRNA            complement(895..971)
                     /locus_tag="VIF63_08295"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(935..937),aa:Glu,seq:ctc)
     gene            complement(1076..1933)
                     /locus_tag="VIF63_08300"
     CDS             complement(1076..1933)
                     /locus_tag="VIF63_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8239421.1"
                     /translation="MPTSPDATLPPPTRPVRKDEIELHERGLYIESWLPERRSRRKPL
                     LFVHGELGGSWAWERFLGYFAGRGWEGHALNLRNHHWSQTADPAELSFESFVDDVGAA
                     IDRLGPTVVVVGHGMGGLLALKAAERHPVSGLVLVAAELPGDIREPTVSHAIREIPEL
                     FGPSLLGWATLPEKLQRDHRDLTLADVLRIQHLMGQKAHESGRARRTMLRGVKVDPAK
                     LAEIPRLVIGGGLDRVVPLASGERLAEWLDAEFEPFGAHSHYGLILGEEGYTQVADAI
                     RGFLEAHRL"
     gene            complement(1941..3281)
                     /locus_tag="VIF63_08305"
     CDS             complement(1941..3281)
                     /locus_tag="VIF63_08305"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY8239422.1"
                     /translation="MPSIRPFRALRYSRDQVDDLAAVVAPPYDVISPDLHRRLLARDP
                     RNVVRLDLPIDEAGDPPDERYRRAGRTLQAWRSDGTLRRDIRAGLYPYEQTFGIPGTE
                     RTATRRGVFARVVLEPFGPDSGIRAHERTLAEPREDRYRLLRATGVNTSPVVGLGADS
                     TGGVAEWLREVAATQPVAELVDDDEVGHRLWLAPFEDEDAETRVVRTVADLLGSAPIT
                     LADGHHRYETALRYCEERRQGPVLEDAPAWAEILMLILEPVEGALTVLPTHRVLLGLE
                     PAAVASLVSRLPELFDVETGVSRERLVAAFGGGSDAAGGEGRFGLWAGGSGAILRARR
                     PAFVPFLPAGGAAVRRLDVTLASVAIERLAGIDPAAVAEGRIAYTMSAAEAIRWVDEG
                     GSPTGTHPASVALLLEPTPAAEIVAVAADGDVMPQKSTYIYPKALTGLVINPLE"
     gene            3408..5900
                     /locus_tag="VIF63_08310"
     CDS             3408..5900
                     /locus_tag="VIF63_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239423.1"
                     /translation="MPPRRPGLRAHSRSPRGILRLASVLGVLVLTLGPLALTGAAAEG
                     LTMDARVLLQGHARAGSWAAIEVDLKNDGPAIQGELRMDGGDQSNARYSMAVNLPTGS
                     RQTYVLHAQPPSFGRSVKVELVSGDQVIESADVAYLMHEASQLVVGILAERPAAIVSQ
                     IHIPANQFGTAPVIVPLTLADLPERSEGWGVLDRLVWQDIDSNQLTSGQLTALRRWLA
                     GGGRLVIVGGSAGIGTLSAFPDDILPYRPTATVDLDPSALVPLLGVVPSAATELPAMG
                     GELARGRAIATSGDRVVAAELAYGNGRVTILGFDPTTPWLAESKSVDPLWRGVLPDRF
                     GGTPVLTDDSQLVQAVYQLPALALPPTSGLLVLIAAYILIIGPINYLVLRRMDRRELA
                     WITMPVLVIGFSAAAFGYGALLRGTDVVINEISIVRGATGATEASADVYFGIFSPTRT
                     TFQVELAQGALLAAPMNGNPFGQSTGTLDLVQGTGTEQPSAVRNLVVGTGSLRVVRAQ
                     VPVEAPLMHADLRLIDGVLTGTFENASDELLEGVAVALGSSVAVLGDVQPHTTKNVRV
                     AVVANPFANGLADQVVGAAFDNSTEEGVRRSARYQMVSQLTSDPFGQFGGTLPADRAV
                     ILAFGRRELLDLKVGAVAPRRSGNILYYVPVGVRITGKITFTGDLLPASVLDADAQFF
                     SKDRTFLNMGAGQATLAYRPIPFDGTFTPSEIRMTLGQGGGFGNAPGGKEIKALPEIP
                     VPCIDVANTTPEGCVEPRNDLIPEVEVFDRRGEGTWVRLPRLNAEANYTLADPTRYVD
                     PATGQMLVRFVNESRELSIGFSFQLSILGAVQ"
     gene            5897..6892
                     /locus_tag="VIF63_08315"
     CDS             5897..6892
                     /locus_tag="VIF63_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239424.1"
                     /translation="MSAIIATQGLVKRYDGTVAVGGVDLEIEEGEIFGLVGPNGAGKT
                     TMLRMLATLLVPTSGDALIAGESVRRNPMAVRRVIGYMPDAFGVYDDMRVWEYLDFFG
                     RCYGLGPAQRRRMIGDLLELVDLADKREAYVQSLSRGMQQRLCLAHALVHDPKVLLLD
                     EPASGLDPRARVELRELLRELRVLGKTIVISSHILPELEELCTSVAIVDHGRLLASGS
                     IAEIGQKLRIGEVLRVRVLGDETAVESARAWFEGRPDVASATTDLSGQIEIGFRGDET
                     GAAALLAAAIRAKIAVASYSPAATDLQELFLQVTDRDAAMTAQAAGAAGGTGAAA"
     gene            6889..>7496
                     /locus_tag="VIF63_08320"
     CDS             6889..>7496
                     /locus_tag="VIF63_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8239425.1"
                     /translation="MTSLAARFPLRGRLPKIGIGSGMSAIIVKELRGRMRGRRAFIVL
                     TIHVLLLAGIAWMLQRINEEAISNFGGFGGQVTFASASVGRGIFIGLMLLQTLMVSVL
                     APAATAGAISSEREHQTLELLTVTPISSLAIVLGKLFSALAWVFVLILASVPVTALVF
                     VFGGVAPDDLLRAYIVLFATVIGLGSVGIFFSALTRRTGAATG"
BASE COUNT         1131 a         2719 c         2576 g         1070 t
ORIGIN      
        1 cgagatcggc acgaggagca ccccgaacga ggcgcgcagg agcgtcactg gaccacccag
       61 gccggggagg accgacagca tcagcggcag cgccgcgcga cccatggcca cgacgaacca
      121 gcgtgcctgc tggcgttcca cgggcgtgcc gcggcgatag cgcacgatca ccgctgccac
      181 gccaagggcg gtcatcgaca cgatgccacc cgccacgccc acataaccca gcgacgcgag
      241 gaacggtggg agcccctgga tgccgatcgg gtttcggtcg ccgattggtc cggcaacgac
      301 tgtcggtgta atgatctgga cgaacgtccc gaatcctgtg atcgtcgccg ccacggcaac
      361 cggcaaggac cagcgcaatg acggcagccg tccgtcgggg aacatgatcg cgagggccgg
      421 caggatcgcg accacgcccg gcccaagcgc caccggtccg atcagcagga gcagttggtt
      481 caccgaggaa tccgggtttg cgaaggtcag gatcccgccg accatgtagc cgaaggggat
      541 gagcacgaac agcgccccgc cggccaggag gacccagccg atccgacccg cccccggtcg
      601 gcccgcgagc agggcgccca cggtcgcata cccgatggcg atggcgcaga aggccgtcat
      661 ggccagggcc agaatgccga tattgtcggc gcccggactt gcccccgcgg tgacgaatgc
      721 cgacatgtcg cgcacgtagg tggtcccgta cgcgacccac aggattccca ggaccatcgc
      781 gagcgtgacg agccagatcg caccggcgag gagggtgacc catcggggcg tcgtcgggcg
      841 cacgtgcgtc acagtcggcc ggctgagtgc atgcgcacct tctgacgaag ggcttggtac
      901 cgcatatcgg attcgaaccg atgatctccg ccttgagagg gcggtgtcct gggcctctag
      961 acgaatgcgg cacggacgcg tcgggaggcg atttgcctcc gagcagggcg gaatgttacc
     1021 agccctcggc cagacggccg ccgcgcgagc cgccgggaga gccgccgcgc gagcgctaca
     1081 gccggtgcgc ctccaggaac ccccggatcg cgtcggcgac ctgcgtgtag ccctcctcgc
     1141 cgaggatcag cccgtagtgc gaatgggcgc cgaacggctc gaactcggcg tcaagccact
     1201 cggccagccg ctcgccggac gcaagcggga cgacccgatc gaggccgcca ccgatgacca
     1261 gccgcggaat ctcggcgagc ttcgcgggat cgaccttcac gccgcgcagc atcgtccgcc
     1321 gagcgcgacc ggactcgtgt gctttctggc ccatgaggtg ctggatgcgc agcacgtcgg
     1381 cgagcgtcag gtcgcgatgg tcgcgctgga gcttctcggg cagcgtcgcc cagccgagca
     1441 gcgacggccc gaacagctcg gggatctcac ggatggcgtg cgacacggtc ggctcgcgga
     1501 tgtcgcccgg cagctccgcg gcgacgagga cgagcccgct gaccgggtgc cgctcggcgg
     1561 ccttcagcgc gaggagcccg cccatgccgt gaccgaccac gacgaccgtc ggaccgagcc
     1621 ggtcgatcgc cgccccgaca tcgtcgacga acgattcgaa cgacagctcg gcgggatcgg
     1681 ccgtctggct ccagtgatgg ttgcgcaggt tgagggcgtg gccctcccag ccgcggcccg
     1741 cgaagtagcc gaggaatcgc tcccaggccc acgagccacc gagctcgccg tggacgaaca
     1801 gcagcggctt gcgccgcgag cgccgctccg gcagccacga ctcgatgtac aggccgcgct
     1861 cgtgcagctc gatctcgtcc ttgcggaccg gccgggtggg cggcggcagc gtggcgtcgg
     1921 gcgaagttgg catcggtcct ctattcgagc ggattgatga ccaggccggt gagggccttc
     1981 gggtagatgt acgtgctctt ctggggcatc acgtcgccat cggcggcgac cgcgacaatc
     2041 tcggcagcag gcgtcggctc gaggaggagc gccaccgagg ccggatgcgt gcccgtcggc
     2101 gagccgccct cgtcgaccca gcggatagct tcggcagccg acatcgtata ggcgatgcgg
     2161 ccttcggcca cggcagccgg gtcgatgccg gccagccgct cgatcgccac cgaggcgagg
     2221 gtcacgtcca gccgccggac cgctgcgccg ccggcaggca ggaacgggac gaacgcgggc
     2281 cgcctcgccc gcaggatcgc gcccgagccg cccgcccaca gcccgaaccg gccctcgccg
     2341 cccgcggcgt ccgagccgcc accgaacgcc gccacgagcc gctcccgcga gacgccggtc
     2401 tcgacgtcga acagctccgg caagcgcgag acgaggctcg ccaccgcggc gggctccagg
     2461 cccaggagca cgcgatgggt cggcaggacg gtcagcgccc cctcgaccgg ctcgaggatc
     2521 agcatcagga tctcggccca tgccggcgcg tcctcgagga ccgggccctg ccgacgctcc
     2581 tcgcaatacc gcagcgccgt ctcgtagcgg tggtgtccgt cggccagcgt gatcggcgcc
     2641 gagccgagca ggtcggcgac ggtgcgaacg acccgcgtct cggcgtcctc gtcctcgaag
     2701 ggtgcaagcc acagtcgatg cccgacctcg tcgtcatcga cgagctcggc gacgggctgc
     2761 gtggccgcga cctcgcgcag ccattccgcg acgcctcccg tcgaatcggc tccgagcccc
     2821 acgaccggac tcgtgttgac gcccgtcgcc cgcaacagcc gatagcggtc ctcgcgcggc
     2881 tcggcgagcg tgcgctcgtg cgcccgaatg cccgagtccg gcccgaacgg ctccaggacc
     2941 acccgcgcga agacgccgcg gcgagtagcg gtccgttccg tgcccgggat cccgaacgtc
     3001 tgctcgtagg gatagagccc cgcccggatg tcgcgccgga gcgtcccgtc ggagcgccag
     3061 gcctgcaacg tccggccggc gcgccgatag cgctcgtcgg gtgggtcacc cgcctcgtcg
     3121 atcggcaggt caagccgtac cacgttgcgc gggtcgcgcg cgagcagacg ccgatgcaga
     3181 tcgggcgaga tgacgtcata cggcggggca acaaccgcgg caaggtcgtc gacctggtcg
     3241 cgggagtagc gcagcgcgcg gaacgggcgg atcgacggca cgggcgggaa tcgtacgata
     3301 gggaccgagc ggcgcaactt ctcggggcct tccggcgtcg ttgcgttcgg ccgccggctc
     3361 atttgctgcc gcgcggccgc caccgaccag ctcgcacagg agaacgcgtg ccgcctcgcc
     3421 gccccggcct ccgagcgcat tcccgatccc cgcgcggcat cctgcgtctc gcatccgtcc
     3481 tcggcgtcct cgttctgacg ctggggccgc tggcccttac cggcgccgcg gccgaaggcc
     3541 tgacgatgga cgcccgggtc ctgctccagg gccatgcacg cgccggtagc tgggcagcga
     3601 tcgaggtcga cctcaagaac gacggaccgg caatccaggg cgagctgcgc atggacggcg
     3661 gcgaccagag caacgctcgc tactcgatgg ccgtcaacct gccgacaggc tcccgccaga
     3721 cgtacgtgct gcacgcgcag ccgccgtcct ttggtcgcag cgtcaaggtc gagctcgtct
     3781 cgggcgacca ggtgatcgaa agcgcggacg tcgcgtacct catgcacgag gcgtcgcagc
     3841 ttgtcgtcgg catcctcgcc gagcggccgg ccgccatcgt cagccagatc cacatccccg
     3901 ccaaccagtt cgggacggcg cccgtgatcg tgccgctgac cctcgccgac ctgcccgagc
     3961 gctcagaggg ctggggcgtc ctcgaccgcc tcgtctggca ggacatcgac agcaaccagc
     4021 tgaccagcgg ccagctcacc gcccttcgac gctggctcgc cggcggcggg cggctcgtga
     4081 tcgtcggtgg cagcgccggc atcggcacgt tgtccgcttt cccggacgac atcctgccgt
     4141 accgcccgac ggccacggtc gacctcgatc ccagtgcgct cgtgccgctg ctcggcgtcg
     4201 tgccgagcgc cgcgacggag ctgccggcga tgggcggcga gctcgcccgg ggtcgcgcca
     4261 tcgccacctc cggcgatcgc gtcgtcgcgg ctgagctcgc gtatggcaac ggccgggtga
     4321 cgatcctcgg cttcgacccg accacaccgt ggctcgccga gagcaagagc gtcgacccgc
     4381 tgtggcgggg cgtcctgccc gaccggttcg ggggtacgcc cgtgctgacc gatgacagcc
     4441 agctcgtgca ggccgtctac cagctgccgg cgctggccct gccgccgacc tccggcctgc
     4501 tggtcctgat cgctgcctac atcctgatca tcggcccgat caactacctg gtcctgcgcc
     4561 ggatggaccg gcgcgagctg gcctggatca cgatgccggt cctcgtcatc ggcttctccg
     4621 cggctgcgtt cggctacggc gcgctcctgc gcggcaccga cgtcgtcatc aacgagatct
     4681 cgatcgtgcg cggagcgacc ggcgcgaccg aggcgagcgc cgatgtctac ttcggcatct
     4741 tctcgccgac ccgaaccacc ttccaggtcg agctcgcgca gggcgccctg ctggcggcgc
     4801 cgatgaacgg caacccgttc gggcagagca cggggaccct tgacctcgtg caaggcaccg
     4861 gaacggagca gccctcggcc gtgcgcaacc tcgtcgtcgg caccggctcg ctgcgcgtcg
     4921 tgcgcgcgca ggtgcccgtg gaggccccgc tcatgcacgc cgacctgcgg ctgatcgatg
     4981 gcgtcctcac cggcacgttc gagaacgcgt cggacgagct gctcgaaggc gtcgcggtgg
     5041 cgctcggcag ctccgtggcc gtcctgggcg acgtccagcc ccacacaacc aagaacgtcc
     5101 gcgtggccgt cgtcgccaat cccttcgcga atggcctggc cgaccaggtc gtcggagccg
     5161 cgttcgacaa cagcacggag gaaggcgtgc gtcgatccgc gcgctaccag atggtctcgc
     5221 agctgacgag cgaccccttc ggccagttcg gtggcacgct gcccgccgac cgggccgtga
     5281 tcctggcctt cggccggcgc gagctgctgg acctcaaggt gggcgcggtt gcgccacggc
     5341 gcagcggcaa catcctctac tacgtgccgg tcggcgttcg gatcaccggg aagatcacgt
     5401 tcaccggcga cctgctgccg gcctcggtgc tcgatgccga cgcgcagttc ttctcgaagg
     5461 accgcacgtt cctgaacatg ggcgccggcc aggcgaccct cgcctaccgg ccgatcccgt
     5521 tcgacggcac cttcacgccg agcgagatcc ggatgaccct cggccagggt ggcgggttcg
     5581 gcaacgctcc gggcggcaag gagatcaagg ccctgccgga gatcccggtg ccctgcatcg
     5641 acgtggcgaa cacgacgccc gaaggctgcg tcgaaccgcg caacgacctc attcccgagg
     5701 tcgaggtgtt cgaccggagg ggtgagggca cgtgggtccg gctgccgcga ctcaacgccg
     5761 aggcgaacta caccctcgcc gacccgacgc ggtacgtgga ccccgcgacc ggccagatgc
     5821 tcgtccggtt cgtcaacgag agccgggagc tcagcatcgg cttctccttc cagctgtcga
     5881 tcctgggggc ggtccagtga gcgcgatcat cgcgacgcag ggcctcgtca agcgctacga
     5941 cgggaccgtc gccgtcggcg gcgtcgatct cgagatcgag gagggcgaga tcttcggcct
     6001 cgtcggcccg aacggcgccg gcaagaccac gatgctgcgg atgctcgcga cgctgctcgt
     6061 gccgacgtcg ggcgatgccc tgattgccgg agaatcggtt cgccggaacc caatggcggt
     6121 tcgtcgcgtc atcggctaca tgcccgacgc ctttggggtc tacgacgaca tgcgggtctg
     6181 ggagtacctg gacttcttcg gtcggtgcta cggcctcggg cccgcccagc ggcggcggat
     6241 gatcggtgac ctgctcgagc tcgtcgacct cgccgacaag cgcgaggcct acgtccagag
     6301 cctgtcgcgg ggcatgcagc agcgcctgtg cctcgcccac gcgctcgtcc acgatccgaa
     6361 ggtgctcctc ctcgacgagc ctgcctccgg cctcgacccg cgcgcgcgag tcgagctccg
     6421 agagctgctt cgggagctcc gggtcctcgg caagacgatc gtcatcagca gccacatcct
     6481 ccccgagctc gaggagctgt gcacatcggt cgcgatcgtc gatcatggcc gcctgctcgc
     6541 gtctggctcc atcgcggaga tcgggcagaa gctccgaatc ggcgaggtgc taagggtgcg
     6601 cgtgctgggg gacgagacgg cagtcgaaag cgcacgcgcc tggttcgaag gccgcccgga
     6661 tgtcgcctcc gcgacgacgg atttgagcgg ccagatcgaa atcggcttcc gcggcgacga
     6721 gacgggcgcc gcggccctgc tcgcggccgc catccgggcg aagatcgccg tcgcgagcta
     6781 ctcgcccgcc gcgacggacc tccaggagct cttcctccag gtcaccgacc gcgatgccgc
     6841 gatgaccgcg caggccgcgg gtgcggctgg cggcaccggg gcggcggcat gacctcgctc
     6901 gcagctcgct tcccgctccg tggccggctg ccgaagatcg gcatcggctc cggcatgtcc
     6961 gcgatcatcg tcaaggagct gcgcggccgg atgcgcggac ggcgggcatt catcgtcctc
     7021 accatccacg tgctgctcct ggcgggcatc gcctggatgc tccaacgaat caacgaagag
     7081 gcgatctcca acttcggcgg gttcgggggc caggtgacct tcgcctcggc gagcgtcggg
     7141 cggggcatct tcatcggcct gatgctcctc cagacgctga tggtgtcggt gctggcgccg
     7201 gcagcaacgg ccggcgcgat cagctccgag cgcgagcatc agacgctcga gctgctgacc
     7261 gtcacgccga tctcgtcgct cgcgatcgtg ctcggcaagc tcttcagcgc cctcgcctgg
     7321 gtgttcgtcc tgatcctcgc gtccgtgccc gtcaccgcgc tcgtcttcgt gttcggcggc
     7381 gtggcccccg acgacctgct ccgggcatac atcgtcctgt tcgcgaccgt catcggcctc
     7441 ggctcggtcg gcatcttctt ctccgcgctg acacgccgga ccggcgccgc gacggg
//
LOCUS       DATBSW010000301         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1052354, whole genome shotgun sequence.
ACCESSION   DATBSW010000301 DATBSW010000000
VERSION     DATBSW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052354"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VIF63_08325"
     CDS             complement(<1..690)
                     /locus_tag="VIF63_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8239426.1"
                     /translation="MIPNDVLALGPFILAVAVALAVLVADLVAPGKREPVLAISLGGL
                     ALVGILTLATGQSVAADAAGTSRVLAFGGAYVVDGLTTFLDLIFVGIVAFTIVFAPDY
                     LEARGLPLAEFSIILLFAMTGAMLIAGAADLLVLFVALELMVLPGYMLAGYHKSDGYS
                     TEGAIKYFLLGSFSSAIFLFGLAFAWGATGTTSIAGVRAALEGVAAGSAALSPALVLG
                     LGFMTTGVAFKI"
     gene            complement(687..>1970)
                     /locus_tag="VIF63_08330"
     CDS             complement(687..>1970)
                     /locus_tag="VIF63_08330"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8239427.1"
                     /translation="GVFLALDTFLFYIFWEVVLVPMYLIIGIWGGQNRIYATIKFVLY
                     TLVGSLLMLVAILATAFAYQGAHGGVWAGSFDFEALRAYAGTTGFADGLQLLSFGAFF
                     LAFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKLGGYGFIRFSLSLYPDASHTY
                     APLIIVLSLVAIIYGAIVAMVQPDLKKLVAYSSVSHMGFVTLGIFVFQEQALDGAILQ
                     MINHGLITGALFLLVGVIYERTHDRTIAKMGGLAALTPVYAATFGFFVFASAGLPALS
                     GFVGEFLTLVGTFVVSPLVAAIATVVMILAAVYLLWMFQRVVLGEPSAFLLGLKHHLT
                     DMTPTEVLTLAPLGALVVIFGLFPGVLLELIQGPVHQALDSVNGGAGITIDPIFTVVA
                     IGFVVAVVAARLLALRPGRPTGDSGSAVQTVEGAA"
BASE COUNT          399 a          622 c          721 g          228 t
ORIGIN      
        1 gatcttgaac gcgacgccgg tcgtcatgaa cccgaggccg agcacgagcg cgggcgacag
       61 ggcggccgag cccgccgcga caccctccag cgccgcgcgg accccggcga tgctcgtcgt
      121 gccggtcgcg ccccacgcga aggcgagccc gaacaggaag atcgcagagc tgaagctgcc
      181 gaggaggaag tacttgatcg cgccctcggt cgagtagccg tcggacttgt ggtacccggc
      241 gagcatgtag cccggcagga ccatgagctc gagggcgacg aagagcacga gcaggtccgc
      301 ggcgccggcg atgagcatcg cgccggtcat cgcgaacagc aggatgatcg agaattcggc
      361 gaggggcagc ccgcgggcct cgaggtaatc gggcgcgaag acgatcgtga acgcgacgat
      421 gcccacgaag atgagatcca ggaaggtcgt caggccatcg acgacgtacg cgccgccgaa
      481 ggcaagcacg cgcgacgtgc cggccgcgtc ggccgcgacc gactgccccg tggcgagcgt
      541 caggatcccg acgagggcga ggccgccgag gctgatggca aggaccggtt cgcgcttgcc
      601 gggcgccacg aggtcggcga cgagcaccgc gagcgccacg gcgaccgcga ggatgaatgg
      661 cccgagggcg agaacgtcgt tcgggatcac gcggcgcctt cgaccgtctg gaccgccgag
      721 ccggaatcac ccgtcggccg tcccggccgg agcgcgagca ggcgcgcggc gacgaccgcg
      781 accacgaacc cgatggcgac caccgtgaag atcggatcga tcgtgatccc cgccccgccg
      841 ttcacggagt cgagggcctg gtggacgggc ccctggatca attcgaggag gacacccggg
      901 aagaggccga agatgaccac gagggcgccg agcggcgcga gggtcaggac ctcggtcggg
      961 gtcatgtcgg tcaggtggtg cttgagcccg agcaggaacg ccgagggctc gccgaggacg
     1021 acccgctgga acatccagag caggtagacc gcggcgagga tcatcacgac cgtcgcgatc
     1081 gccgcgacca gcgggctcac gacgaaggtg ccgacgaggg tcaggaactc gccgacgaag
     1141 ccggagaggg ccggcaatcc ggccgaggcg aacacgaaga agccgaacgt ggcggcgtac
     1201 accggggtga gcgccgccaa tccgcccatc ttggcaatcg tccggtcgtg ggtccgctcg
     1261 tagatcacgc cgacgaggag gaacagcgcg ccggtgatga ggccgtggtt gatcatctgg
     1321 aggatcgccc cgtcgagcgc ctgctcctgg aacacgaaga ttcccagcgt gacgaagccc
     1381 atgtggctga ccgacgagta ggcaacgagc ttcttcaggt ccggctggac catcgccacg
     1441 atcgccccgt agatgatcgc gaccaggctc agcacgatga tcagcggcgc ataggtatgc
     1501 gacgcgtccg gatacagcga caggctgaac cggatgaagc cgtagccacc gagcttgagg
     1561 agcacgccgg cgaggatcac cgagcccgcc gtcggcgctt cgacgtgggc gtcgggcagc
     1621 caggtgtgga acgggaacat cgggaccttg atcgcgaagg ccaggaagaa cgcgccgaag
     1681 ctcagcagct ggaggccgtc ggcgaagccg gtcgtgccgg cgtaggcacg cagcgcctcg
     1741 aagtcgaacg agccggccca gacgccgccg tgggcgccct ggtaggcgaa cgcggtggcg
     1801 aggatcgcga cgagcatcag cagtgagccg acgagcgtgt agaggacgaa cttgatcgtc
     1861 gcgtatatcc gattctggcc gccccagatg ccgatgatca ggtacatcgg cacgagcacg
     1921 acttcccaga agatgtagaa gaggaacgtg tcgagggcga ggaacacgcc
//
LOCUS       DATBSW010000302         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_346651, whole genome shotgun sequence.
ACCESSION   DATBSW010000302 DATBSW010000000
VERSION     DATBSW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346651"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(37..143)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(183..1733)
                     /locus_tag="VIF63_08335"
     CDS             complement(183..1733)
                     /locus_tag="VIF63_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY8239428.1"
                     /translation="MSELLVGIDVGTQSSKGVLLRADGTLVTEARAQHGMDVPRPGWA
                     EQDADSVWWADVCTIARRLAAEVPEGDRIGAVGVSAIGPTVVPLDEAGRPLRTAILYG
                     VDTRATAQIAALEQRHGADALAAWSGMGLSSQATAPKIAWLADHEPEIHAAARWFVSA
                     TTYLVHRLTGAMVVDAHTASHGNPVFDRATLAWSDRYAGGITSIDRLPAIGWPADVAG
                     EVTPEAAAATGLPAGVPVVVGTIDAVAEGFSVGVSRPGDLMIMYGSTTFFILMTEKPI
                     EAGSLWLTAGAERGSWALAAGLATGGSALAWFRDQLAADLVAAERDGGASAFSGLSAE
                     AAAAATVRSAGAAAAGGAGESILFLPYFSGERTPINDPFARGVAAGLSLRTRRGDLYL
                     ALLEGIAYATRANIEAMRDLGAPIRRVVAVGGGTADPLLVQLVSDAAGIDQEIPAQTI
                     GASRGDALLAGLGSGIVARDAVERWMRIDRIVRPQDGARAGHDLRYQAFGELYRSTRD
                     VVHGLGLR"
     gene            complement(1730..>2265)
                     /locus_tag="VIF63_08340"
     CDS             complement(1730..>2265)
                     /locus_tag="VIF63_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239429.1"
                     /translation="VWTALGLDPATTLHDVRWGAQYGDDFVWVFEISGAVPPAHLVGG
                     YAGAVSERQPPVYFGLGGGTIKGVSRPGEIVWSRVFLMDGRLHADLGRATVVELPQAE
                     TERRWQATTPQWPIMHAVLHGVSRDQMMARHRANHIQVAYGDDAPTADRALAAKAAMF
                     EAMGLAVHLCGDVPLGA"
BASE COUNT          307 a          852 c          794 g          312 t
ORIGIN      
        1 aaggcccctc cgctgcggaa gggccatcgg gggctgacct tatctcgcag gaagacctgc
       61 ccggaattgg caccttgccg gctcgaggcc ggtaggttgt cgtggcttca tcgggccggt
      121 ccctccacca ctcttgataa gcgctcttca gttgtccgcg gatgatgccc ggccggaggg
      181 gttcagcgca accccagacc gtgcacgacg tcgcgagtcg agcggtagag ctcgccgaac
      241 gcctggtacc gcaggtcgtg gccggctcgc gcaccgtcct gcggcctgac gattcggtcg
      301 atgcgcatcc accgttcgac cgcgtcccgc gccacgatgc ccgacccgag acctgcgagc
      361 agcgcgtccc cgcgcgacgc gccgatcgtc tgtgccggga tctcctggtc gatgccggcc
      421 gcgtccgaga cgagctggac gagcagggga tcggcggtgc cgccgccgac ggccacgacc
      481 cggcggatcg gcgcgcccag gtcgcgcatt gcctcgatgt tcgcccgggt cgcgtaggcg
      541 atgccctcca ggagtgcgag gtacaggtcg ccgcgccgag tccggagcga caggcccgcg
      601 gccacgcctc gcgcgaacgg atcgttgatc ggggtgcgct cgccggagaa gtacggcagg
      661 aagaggatgc tctcgccggc cccgcccgcc gccgcggctc cggccgagcg caccgtggca
      721 gccgctgccg cctccgccga gaggcccgag aacgccgacg cgccgccatc gcgctcggcc
      781 gccacgaggt ccgcggcaag ttggtcgcgg aaccaggcga gcgccgagcc gcccgtcgcg
      841 aggcccgccg caagggccca gctgccgcgc tcggcgccgg cggtcagcca gagtgagccc
      901 gcctcgatcg gcttctcggt catgaggatg aagaacgtcg tgctgccgta catgatcatg
      961 aggtcgcccg gccgcgagac accgacgctg aacccctcgg ccacggcgtc gatggtcccg
     1021 acgacgaccg ggacgcccgc cggcagtcct gtcgcggcgg ctgcctccgg ggtgacctcg
     1081 ccggcgacgt ccgccggcca gccgatcgcg ggcaggcgat cgatcgaggt gatgcctccg
     1141 gcgtagcggt ccgaccaggc cagggtcgcc cgatcgaaga ccgggttgcc gtgggacgcc
     1201 gtgtgggcgt cgacgaccat cgccccggtc agccggtgca cgaggtacgt cgtggcgctc
     1261 acgaaccagc gcgcagcggc gtggatctcc ggctcgtgat cggccagcca cgcgatcttc
     1321 ggggctgtcg cctggctgga aaggcccatg ccggaccatg ccgcaagggc gtccgcgccg
     1381 tggcgctgct cgagcgccgc gatctgggct gtcgcccgcg tgtcgacgcc gtacaggatg
     1441 gccgtgcgca gcggtcgccc tgcctcgtcc agcggcacga ccgtcggccc gatcgcgctc
     1501 acgccgaccg cgccgatccg gtcgccctcg gggacttccg ccgcgaggcg gcgcgcgatg
     1561 gtgcacacgt ccgcccacca gaccgagtct gcgtcctgct cggcccagcc ggggcgcggc
     1621 acgtccatcc catgctgggc ccgggcttcg gttacgagcg tgccatctgc ccgtagcagc
     1681 acgcccttcg agctctgcgt gccgacatcg atcccgacaa ggagctcgct cacgcgccga
     1741 gcggcacgtc gccgcagagg tggaccgcca ggcccatcgc ctcgaacatc gccgccttcg
     1801 ccgcgagcgc ccggtccgcg gttggtgcgt cgtcgccgta cgcgacctgg atgtggttcg
     1861 cccgatggcg ggccatcatc tggtcgcggc tcacgccgtg cagcaccgcg tgcatgatcg
     1921 gccactgcgg ggtcgtcgcc tgccagcgcc gctcggtctc cgcctgcggg agctccacga
     1981 ccgtcgcccg tccgagatcc gcgtggagcc ggccgtccat caggaagacc cgactccaca
     2041 cgatctcgcc cggccggctc acgcccttga tcgtcccgcc gccgagcccg aagtacacgg
     2101 gtggctggcg ctcgctgacc gcgcccgcgt agccaccgac gaggtgcgcc ggtggcacgg
     2161 cgcccgagat ctcgaagacc cacacgaagt cgtcgccata ttgcgcgccc cagcggacgt
     2221 cgtgcagggt cgtcgctggg tcgaggccga gcgcggtcca gacac
//
LOCUS       DATBSW010000303         9676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_946269, whole genome shotgun sequence.
ACCESSION   DATBSW010000303 DATBSW010000000
VERSION     DATBSW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946269"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VIF63_08345"
     CDS             <1..375
                     /locus_tag="VIF63_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEY8239430.1"
                     /translation="EARARVVTAIPYPSFLALLRGAVVAVSDSGGVQEEGATLGVPVA
                     VTREITERGEGIQEGAAVLVGTDEDRIVDVVGGLLRDDVRRAAMADAGRRLYGDGHAA
                     TRIAEVMAADLATDLAADSRTA"
     gene            372..1679
                     /locus_tag="VIF63_08350"
     CDS             372..1679
                     /locus_tag="VIF63_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY8239431.1"
                     /translation="MSRAVAGNVARLTAVRYLSLFGGFLASVLGARLLGAEGMGIVGL
                     VITVGLLGAVISSAGLSLSAVYFLSRSTDGPRVTVGRLLVLGVIATLVAFAIVGLLGL
                     ALSSLLPDMDRVILLAAPLSASILAFDLGGAILLGLTSETAYIWLAVAEALFGLVLTA
                     VILLFVAATPAGFLVATTLAYAAAAGLGLAIIRRRVGPIHFGWDGVFTRQVLAFGLRG
                     QIGNVLSFLNSRLDLLLVSSFIGLTAAGLYYVAVRISEAVFIVAQAGGTLLYPRVAAQ
                     LDRTSTEATESLVRATLLVVGAGALGVVLLGEPFIRIVFGESFAGAVWAARIVAVAML
                     PFTVHRLLVGDMRGRGRPGLVSASSAIALGATVVLDLVLIPPFGIVGAAVASLVAYSI
                     AAAAMVAFFLRETGVSVRELIPTWRDVQALLRRGVSILATLRR"
     gene            1676..2788
                     /locus_tag="VIF63_08355"
     CDS             1676..2788
                     /locus_tag="VIF63_08355"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY8239432.1"
                     /translation="MTPRRSRVVALGYQGFGNVGDEAILGGIEELLRDEAVDVTTIIG
                     GPAPIAAFASARRIASRRLVPTPRTLLALARADALLVTGGGLLHDHFPVVVPRYLAWT
                     LAARVLRCRVVWVGVGIGPLRRGSSRRLAALALRLSHLVLVRDESSAALAREIGGRVD
                     GVIPDPALFLAPPAWPASLASPASAAAEEGLAVVVREPISDPAAADRLVRVLADLLAE
                     AAAAGRPASILTFAGARDRAIAIRIADAVGERGHPRPRIDELAPDHRAALDRLAQASL
                     VVTVRLHGMILAAVAARPFVAVAYDPKVQGVAGELDAADLVIPLETLTAATLDAGIER
                     AGDPARRGRIAARVAALRERRGGIGAEIGTALRGER"
     gene            2785..3966
                     /locus_tag="VIF63_08360"
     CDS             2785..3966
                     /locus_tag="VIF63_08360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8239433.1"
                     /translation="MTRASGGAPTRVVALYGLGYEPDVRLRKTTQSLAAAGFDVTIVA
                     WDRSGALPERERDGPVGVERIGVRSRRGRGLPQLFFLARAGVAMVRRLRRRRPDVVHA
                     VDLPMLLVAVLARPFIRPVHIVYDAYEIYSIMVAHRFPRPVLGALRLLERRLPRAADL
                     VVSAGASRAAWFREFGIESIVVPNWVDPPSIDESDHARQAARAALGVGPDQFCLAYTG
                     ALLASRDLEALVRYAGRHPDDAVLVAGIGDREAWLASATADLPNVKLLGWLPDPTPVL
                     QAADAMYYAVHAEHPYASYAAPNNLYVAIAHAVPLVFRAQGEFVKVAAEREIGRPFDD
                     DASMDAATAELRDPIVSSRIRAALGGLRASYTWDAAVAPLLSAYPRIERPMNSASANG
                     P"
     gene            complement(3876..5399)
                     /locus_tag="VIF63_08365"
     CDS             complement(3876..5399)
                     /locus_tag="VIF63_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY8239434.1"
                     /translation="MSSESTPLTGTPATSKAGTPFALPVSIPVPTLVGLASLFVAAAL
                     QVLGPASWAGVGLAAVAALLLGLDRRFGPLGGGLIVLLALPYGRAADGLAVQLANLPV
                     RPADAAVAVAVLVTLARWRTFVGWRTIAVNDRAPLLLLGAVLGLGVMALVIGIAVGQD
                     PRDIGRDLRWWLLYGALGLAIVLGSTRAQLFSAFRIGTTIFALVVVTTAVLPAFTDGL
                     KHGVLVYDLGLLRMQFGNSVFLLPVIGFVAYGILQRPRAIDVALLALLSGTVVLSLTR
                     TSILTMAGMIGLMIVVAYLLPELRGRRPRVRQALLVVVTTAVAGAAAIGLAVVGTPAK
                     ASGPIIPGNPAVAVSPLDRVLFQDAGTNLNATVSEQGGRFATYRNAVALIAKSPIVGH
                     GFGSLVAVPFAYDATRAATLDMQPGVDDAYLTIAMKAGLLGVIAFTLLLAYAVRMVIS
                     DRGERAWLLPAWAAIVVLTITQSFAVSNYGPFALALFIGLSIRGYADSRGATAASQV"
     gene            5559..6185
                     /locus_tag="VIF63_08370"
     CDS             5559..6185
                     /locus_tag="VIF63_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY8239435.1"
                     /translation="MAPSAVLGANVELGDDADIGEFCQLGVATSAGGTGSGALRVGPS
                     ARIRSHTVIYAGNTIGARFQTGHGVLVREANEIGDDVSIGSHTVVEHHVTIGDGARIH
                     SNAFIPEYTTIEADVWIGPCVVLTNAMYPQSPRAKENLRGPILRRGAKIGANATLLPG
                     VVVGRDALVGAGAVVVRDVPDGAVVVGNPARVTGRVDDIPAYQQEDPA"
     gene            6182..7306
                     /locus_tag="VIF63_08375"
     CDS             6182..7306
                     /locus_tag="VIF63_08375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8239436.1"
                     /translation="MTNAPTATGRIPLVDLAAQYPEIRAEVDAAIARVLDSTAFILGR
                     EVADFEAAFAAHVGATGCVGVASGTAAIELALRACGVGAGDEVITAAHTFIATAEAIS
                     HTGARPVFVDIDPVTFNLDPAAVEAALTARTRAIVPVHLYGQPADMTALMALARAHDL
                     YVIEDAAQAHGAEVDGRRCGSLGDLAAFSFYPGKNLGAYGDAGAVTGTNEALLARVRK
                     LRDHGRTSKYEHDEVGFGERMDALQAAILGVKLRHLEAWTERRRAHARRYDELLEELP
                     VELPREAPGRRHVYHLYVVRAERRDELLAHLKASGIDAGIHYPVPLHLQPAYADLGYA
                     PGSLPETERAAREIVSLPMYPELADAQLVAVADAIRSFDA"
     gene            7303..8361
                     /locus_tag="VIF63_08380"
     CDS             7303..8361
                     /locus_tag="VIF63_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8239437.1"
                     /translation="MTVNIALVGLGYWGPNLARNLALVKGGRLSVLCDARPEQLSRYG
                     ALYPNARTTTDYDAVLADEGVDAVVLATPAPTHHPLASAALRAGKHVLVEKPLATSVA
                     DCEDLIQLAAAGGRTLMVGHVFIYNAAVRKVREYIDSGELGEIRYLYSQRLNLGQVRR
                     DANALWNFAPHDLSIMGYWLRAEPIDVVARGYDYLQPGIEDVVFMTLDYPGGIGANVH
                     ISWLDPLKVRRMTVVGSEKMVVYDDVSTDARVMLYDKGVTRRGPDGAEPSLGRFESFA
                     EFQLLIRAGDVLIPQIEFSEPLRVETQHFVDCIVSGATPETDGQDGLRVVRALEMAQR
                     SMDAHPTRPGASSLRGEP"
     gene            complement(8358..>9676)
                     /locus_tag="VIF63_08385"
     CDS             complement(8358..>9676)
                     /locus_tag="VIF63_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY8239438.1"
                     /translation="WSTAPFDFGKNSASSLPTSIFQDTNTWLWEQASLSALGTRASKG
                     KGGYWYDAANDRLYVSAVSSSGTTAGTDPTAHQVDVPMRSAFLLDGRSGADYIQIRGF
                     EVRHSANGIAYLYGADNGTVADNVLTGNIYMGISVSGYQTTSGPDPADGMTIWRNRGS
                     WNTIELIKLTSGVTNASVCYNDASFNGLAGILVEGPPSGSSYTGKTTGVTLCFNKLHD
                     HEFNPTGQAYNNAAGALIQNGALSVTVRDNSLWNNDVGLHITQGATNMPAMNGLTITR
                     NLIWGNNRFGLYFLDGYRGSGLGTATASYDLIWGNGVGVMVDRGSKNKTLSHETIYAN
                     TGDGIHVGGYQVAPASVAITRSLVTGNGGYGLWLVTGNTASLSYTGFSSNVKGNITGS
                     PSKTAVNTQAPGYMSTLPSSASYLKIGGSSYQYTAGPGSTPIGAKY"
BASE COUNT         1311 a         3422 c         3458 g         1485 t
ORIGIN      
        1 gaggccaggg cgcgcgtcgt cacggccatc ccgtacccga gcttcctggc gctgctgcgc
       61 ggcgccgtcg tcgcggtcag cgactcaggt ggagtgcagg aggagggcgc gaccctgggc
      121 gttccggtcg cggtgacgcg cgagatcacc gagcgcggcg agggcatcca ggagggcgcg
      181 gcggtcctcg tcgggaccga cgaggatcgc atcgtcgacg tggtcggagg cctgctgcgc
      241 gacgacgtgc ggcgggcggc gatggccgat gccgggcgac gcctgtacgg cgacggacac
      301 gccgcgacgc gcatcgccga ggtcatggcc gcggacctgg ccacggacct ggccgcggat
      361 tcgcgcacgg catgagtcgg gcggtcgccg gcaacgtcgc ccgcttgacc gcggttcgct
      421 acctgtccct gtttggcggg ttcctggcaa gcgttctcgg cgcgcggctc ctcggcgccg
      481 agggcatggg catcgtcgga ctcgtgatca cggtcgggct cctcggcgcg gtgatcagca
      541 gcgcggggct gagcctctcg gcggtctact tcctcagccg aagcacggac gggccgcgcg
      601 tgacggtcgg ccgcctcctg gtgctcggcg tgatcgcgac gctggtcgcc ttcgccatcg
      661 tcgggctgct cgggctcgcc ctgtcgtccc tcctgcccga catggatcgg gtgatcctgc
      721 tcgccgcgcc gctgtccgca tcgatcctcg ccttcgatct cggtggcgcg atcctgctgg
      781 gcctgacgtc ggagacggcc tacatctggc tcgcggtcgc cgaggcgctg ttcgggttgg
      841 tcctgaccgc cgtcatcctc ctgttcgtcg ccgcgacgcc ggccggcttc ctcgtggcca
      901 cgacccttgc ctacgcggcc gccgccgggc tgggcctggc gatcatccgc cggcgggtgg
      961 gacccatcca ctttggctgg gacggtgtgt tcacgcggca ggtgctcgcc ttcgggctgc
     1021 gtgggcagat cggcaacgtg ctgtcgttcc tcaactcccg gctcgatctc ctgctcgtgt
     1081 ccagcttcat cggcctgacc gcggccggcc tgtactacgt cgccgtgcgg atctccgagg
     1141 ccgtcttcat cgtcgcccag gccggcggca cgctgctcta cccgcgcgtg gccgcgcagc
     1201 tcgatcggac ctcgaccgag gcgaccgagt cgcttgtccg tgcgacgctc ctggtcgtcg
     1261 gagcgggcgc cctcggcgtc gtgctgctcg gcgagccgtt catccggatc gtcttcggtg
     1321 agtcattcgc cggcgccgtc tgggcggcgc ggatcgttgc cgtcgcgatg ctgccgttca
     1381 ccgtccaccg gctgctcgtc ggcgacatgc gcggacgcgg tcgaccgggc ctcgtctcgg
     1441 ccagctcggc gatcgccctc ggcgcgacgg tggtgctcga cctggtcctc atcccgccgt
     1501 tcgggatcgt cggcgccgcg gtcgcctcgc tggttgccta ctcgatcgcg gcggcggcaa
     1561 tggtcgcgtt cttcctccgc gagacggggg tgtccgtccg ggagctcatc cccacctggc
     1621 gcgacgtgca ggcgctgctg cgccgaggcg tgtcgatcct ggcgaccctc cgtcggtgac
     1681 gccgcgtcgc agccgggtcg ttgccctcgg gtaccagggc ttcggcaacg tgggtgacga
     1741 ggccatcctc ggcggcatcg aggagctgct ccgagacgag gcggtcgacg tcaccacgat
     1801 catcggcggg ccggcaccga tcgccgcgtt tgccagcgcc aggcggatcg ccagccgccg
     1861 gctcgtgccg acgccgcgga ccctgctcgc cctggcgcgc gccgacgccc tgctggtcac
     1921 gggcggcggt ctgctccatg accacttccc ggtggtcgtc cctcgctacc tggcgtggac
     1981 gctggcagcc cgggtcctgc ggtgccgggt tgtctgggtg ggcgttggca tcggaccgct
     2041 ccggcgcggc tcgagtcgtc ggctcgcggc gctggcactg cggctgtccc atctcgtcct
     2101 ggtccgcgac gagtcgtccg cggcgctcgc gcgcgagatc gggggacgtg tcgacggcgt
     2161 gattccggac cccgcgctct tcctggcgcc gcccgcgtgg cctgcgtcgc tcgcgtcgcc
     2221 cgcgtctgcc gcggccgagg aggggctggc ggtcgtcgtc cgggagccga tctcggaccc
     2281 cgcagccgcc gaccggctgg tgcgcgtcct ggccgacctc ctcgccgagg cggcggcggc
     2341 cggacgcccg gcctccatcc tcacattcgc cggggcgcgc gaccgggcca ttgcgatacg
     2401 catcgccgat gcggtggggg aacggggcca tccacggcca cgcatcgacg agctggcgcc
     2461 ggatcaccgt gccgcccttg accgcctcgc gcaggcgagc ctggtggtca cggtccggtt
     2521 gcacggcatg atcctcgccg cggtggctgc ccgccccttt gtcgccgtcg cttacgatcc
     2581 caaggtgcag ggcgtggccg gcgagctcga cgcggcggac ctcgtcatcc cgctcgaaac
     2641 gctgacggct gccacgctcg acgccggcat cgaacgtgcc ggcgaccccg cgcgccgggg
     2701 ccggatcgcg gcgcgggtgg ccgccctccg cgaacggcgc ggcgggatcg gcgcggagat
     2761 cggaaccgcc ctgcgaggcg aacgatgacg cgagcgagcg ggggagcgcc gacccgggtg
     2821 gtcgccctct acgggctcgg ctacgagccg gatgtccgtc tccgcaagac cacgcagtcg
     2881 ctcgccgccg ccggcttcga cgtcacgatc gtggcctggg accggagcgg cgcgttgccc
     2941 gagcgggagc gtgacggccc ggttggcgtc gagcgcatcg gcgtccgcag ccgccggggt
     3001 cgcgggctgc cccagctctt cttcctcgct cgggcgggtg tcgcgatggt ccggcggctg
     3061 cgccgtcgac gacccgacgt cgtccacgcg gtcgacctgc cgatgctcct ggtggccgtg
     3121 cttgcgcgac cgttcatccg gccggtgcac atcgtgtacg acgcctacga gatctactcg
     3181 atcatggtcg cgcatcggtt cccccggccg gtcctcgggg cgctgcgact cctcgagcgc
     3241 cggctgccgc gcgcggccga cctcgtggtc agcgccggcg ccagccgcgc ggcgtggttc
     3301 cgggagttcg ggatcgagtc gatcgtcgtg cccaactggg tcgacccacc gtccatcgac
     3361 gagtccgatc acgcccggca ggcagcgagg gccgccctcg gcgtcgggcc cgaccagttc
     3421 tgcctcgcct acaccggcgc gctcctggcg tcgcgcgacc tcgaggcgct cgttcgatac
     3481 gccgggcggc atccggatga cgccgtgctg gtggcaggca tcggtgaccg ggaggcatgg
     3541 ctcgcgagcg cgacggccga cctgccgaac gtcaagctcc tcggctggct gcccgacccg
     3601 acgccggtgc tgcaggctgc cgatgccatg tactacgccg tgcacgcgga gcatccgtac
     3661 gcgtcctacg cggccccgaa caacctgtac gtcgcgatcg cccacgcggt gccactggtg
     3721 ttccgtgcgc agggagagtt cgtcaaggtc gccgccgagc gcgagatcgg gcgaccgttc
     3781 gacgacgacg cgtcgatgga cgccgcgacg gccgagctgc gcgaccccat cgtctccagt
     3841 cgaatccgag ccgcgctcgg tggcctgcgc gccagctaca cctgggacgc ggcggtcgca
     3901 cccctgctgt ccgcgtatcc ccggatcgag aggccgatga acagcgcaag cgcaaacggc
     3961 ccgtagttgc tcacggcaaa cgactgcgtg atcgtcagca cgacgatggc cgcccatgcg
     4021 ggcagcagcc aggcacgttc gccgcgatcg ctgatgacca tcctgactgc gtacgccaac
     4081 aggagcgtga aggcgatgac cccgagcagc ccggccttca tcgcgatggt caggtaggca
     4141 tcgtcgacgc ccggctgcat gtcgagcgtc gcggcgcgcg tcgcgtcata ggcgaagggg
     4201 accgccacga gcgacccgaa gccatgcccc acgatcggcg atttcgcgat gagcgcgacg
     4261 gcgttgcggt aggtggcgaa gcgaccgccc tgctcggaca cggtcgcgtt caggttggtg
     4321 cccgcgtcct ggaacaggac gcgatcgagc ggtgagacgg cgaccgccgg gttccccggg
     4381 atgatcgggc cactggcctt cgccggcgtg cccaccaccg cgaggccgat ggcagcggca
     4441 ccggcgaccg cggtcgtgac gacgacgagc agggcctgcc tgacgcgcgg ccggcggccg
     4501 cggagctcgg gcagcaggta ggcgaccacg atcatcaggc cgatcatgcc cgccatcgtc
     4561 aggatcgacg tccgggtcag ggacagcacg acggtgcccg agagcagggc gagaagcgcg
     4621 acatcgatcg ccctcggtcg ttgcaggatc ccgtacgcca cgaagccgat gaccgggagc
     4681 aggaacaccg agttcccgaa ctgcatccgg agcagcccca ggtcgtagac cagcacgccg
     4741 tgcttcagcc cgtcggtgaa ggccggcagg acggccgtgg tcaccacgac gagcgcgaag
     4801 atggtggtcc cgatcctgaa cgcgctgaac agctgcgcac gggtcgatcc gaggacgatg
     4861 gccaggccga gcgccccgta caggagccac cagcggaggt cacgcccgat gtctcgtgga
     4921 tcctggccga cggcgattcc gatcacgagc gccatcacgc ccagacccag gacggcgccg
     4981 agcaagagga gcggggcgcg atcgttgacg gcgatcgtgc gccagcccac gaaggtgcgc
     5041 cagcgagcga gcgtgacgag gacggccacc gcgacggctg cgtcggccgg ccgcacgggc
     5101 aagttcgcga gctggacggc cagcccgtcg gccgctcgcc cgtacggcag ggccaggagc
     5161 acgatgaggc cgccaccgag gggtccgaag cgccgatcga ggccgagcag cagcgccgcg
     5221 accgcggcca gcccgacgcc ggcccagctg gccggtccga gcacctgcag cgccgccgcc
     5281 acgaacaggc tggcgagccc gacgagggtc ggcacgggga tcgataccgg cagcgcgaac
     5341 ggtgtgccgg ccttgcttgt cgcgggcgtg ccggtcaggg gcgtcgattc gctgctcacg
     5401 aggaggcgga gtatatggtc tcgcccgagc gatccggcgc gtggggggtc gtcatccgcc
     5461 gcgcgacgat gctcccgtag acttggtcgg cgtccatcgg tcctgcacca gcagccgtat
     5521 cgcagtgcga ggcgtccccg tccctgatgc cgacctccgt ggctcccagc gcagtcctcg
     5581 gcgccaatgt cgagcttggt gacgacgcgg acatcgggga gttttgccag ctcggtgtcg
     5641 cgacatcagc tggcggaacc ggcagcggcg ccctccgggt cgggccttcg gcgcgcatcc
     5701 ggtcacacac cgtgatctat gccggaaaca cgatcggggc acgcttccag accgggcacg
     5761 gcgtcctggt ccgcgaggcc aacgagatcg gcgacgacgt gagcatcggc tcgcacaccg
     5821 tggtcgagca tcacgtgacg atcggggatg gcgccaggat ccattcgaac gccttcatcc
     5881 cggagtacac gacgatcgag gcggacgtct ggatcgggcc ctgcgtggtg ctcacgaacg
     5941 ccatgtaccc gcagtcgccc cgggccaagg agaacctgcg cgggccgatc cttcgccgcg
     6001 gcgccaagat cggcgccaac gcgacgctgc tgcccggtgt tgtggtcggc cgcgatgcgc
     6061 tggtcggtgc cggcgccgtg gtcgtccgcg acgtgcccga cggcgccgtc gtcgtgggca
     6121 atccggcaag ggtcaccggc cgcgtcgacg acatcccggc ataccagcag gaggatccgg
     6181 catgaccaac gcgccgaccg cgacgggtcg gatccccctc gtcgacctgg ccgcccagta
     6241 cccggagatc cgggccgagg tcgacgcggc gatcgctcgc gtgctcgatt ccacggcctt
     6301 catcctcggc cgcgaagtgg cggacttcga ggccgccttc gcggcgcacg tgggcgcgac
     6361 gggttgcgtc ggcgtcgcct cggggacggc ggcgatcgag ctcgcgctgc gcgcctgcgg
     6421 ggtcggcgcc ggcgacgagg tcatcaccgc cgcgcacacg ttcatcgcca cggccgaggc
     6481 gatctcgcac acgggcgccc ggccggtgtt cgtggacatc gatccggtga cgttcaacct
     6541 cgatcccgcc gcagtggagg ccgcgctcac cgcgcgaacg cgcgcgatcg ttccggtgca
     6601 cctgtacggc cagccggccg acatgaccgc gctcatggcc cttgcccggg cgcacgacct
     6661 gtacgtcatc gaagatgccg cccaggccca cggcgccgag gtcgacgggc gtcgctgcgg
     6721 gagcctgggg gacctcgccg cgttcagctt ctatccgggc aagaaccttg gcgcctacgg
     6781 cgacgccggt gccgtgaccg ggacgaacga agcgctcctc gcccgcgttc gaaagctccg
     6841 cgaccacggc cgaacctcga agtacgagca cgacgaggtc ggcttcggcg agcggatgga
     6901 cgcgctccag gcggccattc tcggggtcaa gctccggcac ctcgaggcct ggaccgaacg
     6961 ccgccgggcg cacgcgcggc gttacgacga gctcctcgag gagctgcccg tcgagctgcc
     7021 cagggaggca ccgggccggc gtcacgtcta tcacctctat gtcgttcggg ccgagcggcg
     7081 cgacgagctg ctcgcgcacc tgaaggcgtc cggcatcgac gccggaatcc actacccggt
     7141 gccgctccac ctccagcccg cctatgccga cctcggctat gccccgggct ccctgcccga
     7201 gacggagcgg gcggcgcgcg agatcgtgtc cctgcccatg tacccggagc tcgcggacgc
     7261 gcagctcgtc gccgttgccg acgcgatccg gtcgttcgac gcgtgacggt caacatcgcg
     7321 ctcgtcgggc tcggctactg ggggcctaat ctcgcgcgca acctggcgct cgtgaagggc
     7381 ggtcgcctga gcgtcctgtg cgatgcgcgg cccgagcagc tgagccggta cggagcgctc
     7441 tatcccaacg cccggacgac caccgactac gacgccgtcc tggccgacga gggtgtcgat
     7501 gccgtcgtcc tcgccacccc agcgcccacc caccaccccc tggccagcgc cgcgctgcgt
     7561 gccggcaagc acgtcctcgt cgagaagccg ctcgcgacga gcgtcgccga ctgcgaggac
     7621 ctgatccagc tcgcggcagc gggcggccgg accctcatgg tcgggcacgt gttcatctac
     7681 aacgccgcgg tccgcaaggt ccgggagtac atcgacagcg gcgagctggg cgagatccgg
     7741 tacctgtact cgcagcggct gaacctcggc caggtccggc gtgacgcgaa tgcgctgtgg
     7801 aacttcgcgc cgcacgacct gtcgatcatg ggctactggc tgcgcgcgga gccgatcgac
     7861 gtcgtcgccc ggggctacga ctacctgcag ccgggcatcg aggacgtcgt gttcatgacc
     7921 ctcgactacc ccggcgggat cggcgccaac gtgcatatct cgtggctgga cccgctcaag
     7981 gtgcggcgca tgacggtcgt cggcagcgag aagatggtcg tctacgacga cgtcagcacc
     8041 gacgcccggg tcatgctcta cgacaagggc gtcacgcgcc gtggcccgga cggggccgag
     8101 ccgtcgctgg gccggttcga gtccttcgcc gagttccagc tgctgatccg cgccggcgac
     8161 gtgctgatcc cgcagatcga gttcagcgag ccgctgcggg tggagaccca gcacttcgtc
     8221 gactgcatcg tcagcggcgc gacgccggag accgacggcc aggacgggct gcgcgtggtc
     8281 cgcgccctcg agatggcgca gcgttcgatg gacgcccacc cgacgcggcc gggagcgtcg
     8341 agccttcggg gcgagcccta gtacttcgcc ccgatcgggg tcgatccggg gccggcggtg
     8401 tactggtacg aagagccgcc gatcttgagg tagctggccg aggacggcag cgtcgacatg
     8461 tagcccggtg cctgcgtgtt gaccgcggtc ttcgatggac tgccggtgat gttgcccttg
     8521 acgttgctcg agaagccggt gtacgagagt gacgccgtgt tcccggtcac cagccatagc
     8581 ccgtagccgc cgttgcccgt cacgagggag cgggtgatcg cgaccgaggc cggcgccacc
     8641 tggtacccgc cgacgtggat cccgtcgccc gtgttggcgt agatggtctc gtggctgagg
     8701 gtcttgttct tcgacccgcg gtcgaccatg accccgaccc cgttgcccca gatcaggtca
     8761 taggacgcgg tcgcggtgcc gaggcccgag ccgcgatagc cgtcgaggaa gtacaggccg
     8821 aagcggttgt taccccagat caggttgcgc gtgatcgtca ggccgttcat cgcgggcatg
     8881 ttcgtggcgc cctgcgtgat gtggaggccc acgtcgttgt tccagagcga gttgtcgcgg
     8941 accgtcacgg acagcgcgcc gttctggatc agggcgccgg cggcgttgtt gtaggcctgg
     9001 cccgtcgggt tgaactcgtg gtcgtgcagc ttgttgaagc acagcgtgac cccggtcgtc
     9061 ttgcccgtgt agctggatcc cgacggcggc ccttcgacca ggatcccggc aaggccgttg
     9121 aacgaggcgt cgttgtagca aaccgaggcg ttcgtgacgc cgctcgtcag cttgatcagc
     9181 tcgatcgtgt tccacgagcc gcggttccgc cagatcgtca tgccgtctgc cggatctggg
     9241 ccgctcgttg tctggtagcc cgaaaccgag atacccatgt agatgttgcc cgtgaggacg
     9301 ttgtcggcaa ccgtgccgtt gtccgcgccg tacaggtacg cgatgccgtt ggccgagtgg
     9361 cggacctcga agccgcggat ctggatgtag tccgcgccgg accgtccgtc gagcaggaac
     9421 gcggaacgca tcgggacgtc gacctggtgg gcggtcgggt cggtgcctgc ggtcgtgccg
     9481 gagctcgaca cagcggacac gtacaggcgg tcattcgccg cgtcgtacca gtagccgccc
     9541 ttgcccttcg aagcccgggt accgagcgcg ctgaggctgg cctgctccca cagccacgtg
     9601 ttggtgtcct ggaagatcga ggtcgggagc gagcttgcgc tgttcttgcc gaagtcgaac
     9661 ggggcggtcg accagg
//
LOCUS       DATBSW010000304         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_135439, whole genome shotgun sequence.
ACCESSION   DATBSW010000304 DATBSW010000000
VERSION     DATBSW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135439"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..228)
                     /locus_tag="VIF63_08390"
     CDS             complement(52..228)
                     /locus_tag="VIF63_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239439.1"
                     /translation="MEFFAGAGLVFVVVVLLMWRSRRGTTGRDSLTEGLANDHRDKYA
                     GTNSQPEHTKSWRR"
     gene            complement(347..769)
                     /locus_tag="VIF63_08395"
     CDS             complement(347..769)
                     /locus_tag="VIF63_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEY8239440.1"
                     /translation="MSDLRITVGDLAFDANWERAAPQTIEAIRRMLPIDSNLIHCRWS
                     GESTWIPYGEFRPGIDYENHTSHPSPGMLAMYPGGISECEIFFPYGACTTASKVGPLA
                     ANHFASIVPDDGWQDRLREVGRRALWEGAQAIRIEEGG"
     gene            817..1170
                     /gene="uraH"
                     /locus_tag="VIF63_08400"
     CDS             817..1170
                     /gene="uraH"
                     /locus_tag="VIF63_08400"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY8239441.1"
                     /translation="MTEAQPTISTHVLDTETGMPAAGVHVSLYKLGEDARPIRLTQAL
                     TDADGRIRDLLERPMSPGDYRLEFVLSGRTTPEASDDRFFRRITVDLRVADVGRSYHV
                     PLLVSPFSMTTYRGS"
     gene            complement(1172..1468)
                     /locus_tag="VIF63_08405"
     CDS             complement(1172..1468)
                     /locus_tag="VIF63_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239442.1"
                     /translation="MTRLIVRDEREQAIDNAADRFGFLVVCYGALVVAAYRSFVLGEA
                     AWDLLGLVVLGGVAGLGYRLRQGVVVRRWSIVLVLAVLGAAAVAAAFVWFGPAG"
     gene            complement(1465..1692)
                     /locus_tag="VIF63_08410"
     CDS             complement(1465..1692)
                     /locus_tag="VIF63_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8239443.1"
                     /translation="MASVRGPRLINRLRVARAERSISQTELAHAVDVSRQTISSIETG
                     QYGPSALLAFRLAAALDMAIADLFWIEGADR"
     gene            1834..3090
                     /locus_tag="VIF63_08415"
     CDS             1834..3090
                     /locus_tag="VIF63_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239444.1"
                     /translation="MEARIVSFPSPLAGVRVAGDRIDVDRVSLTDGPLAAWLAAQPAD
                     DHAVLIERAIRIGLTALQSVGVTLNVDAVRAEFDSFVLQNNAANERAAEALEGVLRAN
                     FADGDGRLPRTLETFLGDRGKLQSMVNELFDPNRRDSAIGRISGMLDTYFDGDASRLA
                     RLLDPTRLGSPLHQFRSEVADGFKALHEKLVAIEAAQKARSQERSKSAAKGGDFEDLL
                     EGMLGDIARGSSLLLERTGTDAGDAGRSKKGDFVLTLDPDVCRGADVRIVVEAKDRKM
                     SGREMRDELRAAKTNRDAAAALVVFTPAHAPSGIAPFDVRAGDVYAVIDPADPDFATL
                     EAAVRLARLVAVATLRENEAEVDLGEVQAALTGIRETLDLVRGLKVQLTSIGTTSEAV
                     SKALDKLREAVIAWISRAERELATAP"
     gene            3302..3574
                     /locus_tag="VIF63_08420"
     CDS             3302..3574
                     /locus_tag="VIF63_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239445.1"
                     /translation="MKKRVAAAILWAAVGWVGGELVTGIFGAPSFIALIPAVLMPAFV
                     LWDPTGALWGRSFTGKLIVRPINELADELDARNAPPPSAEAERISR"
     gene            3714..4646
                     /locus_tag="VIF63_08425"
     CDS             3714..4646
                     /locus_tag="VIF63_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8239446.1"
                     /translation="MWKGAIQFGLVTIPVKLYIATESRAGISFNMLHEEDLSRIQMKI
                     FCPVEEEIIDRKDTVKGYEYSPDKYVVIDDEDLEKLPLKTVRSIEIEQFVDRSEAETH
                     TKFVKQAYYIEPDRVGRKAFALLKDVLADKGLTAICKIVIRDREALAALDPFENTMLL
                     STLYWPDEIRALGDLDLPDEEPEIKPAEKKMAAQLIEAMTGEFDPTQYRDEYREALMQ
                     VIESKIEGRETVEIEPAAEPTKLVDLMAALQASVNAATTARESKGGKPVSVAEAKEQR
                     AAKGKATVKASSSKGKADAEDETEERPARRRKSA"
BASE COUNT          825 a         1616 c         1601 g          707 t
ORIGIN      
        1 ctctcgaaca gccggatcat cgtgaagaac aagcgcttca tctcaccatc attagcggcg
       61 ccaggacttc gtgtgttctg gctgactgtt cgtcccggcg tacttgtccc gatggtcgtt
      121 tgcgagacct tccgtcagcg agtcccggcc ggtcgtccct cgccgactcc gccacatgag
      181 gagcacgacg acgacgaata caaggccggc gcctgcgaag aactccatca gcggtcgccg
      241 tcggggcgct tgccgctgtt gctcgagatg acccacagca ggatcccgac gatcacgacg
      301 agcaggatcg cgccgccgac gacaaagatg agccctgaca cgtccatcag cctccctcct
      361 cgatccggat ggcctgcgca ccttcccaga gggctcgccg gccgacctcc cggagtcgat
      421 cctgccagcc gtcgtccggg acgatcgacg cgaaatggtt ggccgcgagc gggccgacct
      481 tcgatgccgt cgtgcatgcg ccgtacggga agaagatctc gcactcgctg atcccgcctg
      541 ggtacatcgc gagcatgccc ggcgaggggt gcgaggtgtg gttctcgtag tcgatgcccg
      601 gccggaactc gccgtacggg atccaggtcg actcgccgga ccaacggcaa tggatcaggt
      661 tggagtcgat cgggagcatc cgccggatcg cctcgatcgt ctgcggcgct gcccgctccc
      721 agttcgcatc gaacgccagg tcgccgaccg tgatccgaag atcgctcatg cgcctgagca
      781 tacggtgccc gccggcgggc gcgtaccctg cgctcgatga ccgaggccca gccgacgatc
      841 tcgacccacg tgctcgacac cgagacgggc atgcccgccg ccggcgtgca cgtctccctg
      901 tacaagctcg gcgaggacgc tcggccgatc cggctgacgc aggcgctgac cgatgccgac
      961 ggccgcatcc gcgacctcct cgagcgcccg atgtcgccgg gcgactatcg gctcgagttc
     1021 gtcctgtccg gccgaacgac gcccgaggcg agcgacgatc gattctttcg gcgaatcacc
     1081 gtcgacctgc gggtcgccga cgtcggtcgg tcgtatcacg tgcccctgct ggtctcgccc
     1141 ttctcgatga ccacataccg gggcagctga cctagccggc gggaccaaac cagacgaagg
     1201 ctgcggcgac agctgccgcg ccaagaaccg ccagcaccag cacgatcgac cagcgccgaa
     1261 cgaccacgcc ctggcgcagt cgatagccga ggcccgcaac gcccccgagc accacgagac
     1321 caaggagatc ccacgccgcc tcgccgagca cgaacgagcg gtacgcggcc acgacgaggg
     1381 ccccgtagca cacgacgagg aagccgaagc gatcagcggc gttgtcgatc gcctgctccc
     1441 gctcgtcacg aacgatcagt cttgtcacct gtcggctcct tcgatccaga agaggtcggc
     1501 gattgccatg tcgagcgcgg ccgccagccg gaatgccagc agcgccgaag gaccgtactg
     1561 gcccgtctcg atcgagctga tcgtctgccg gctgacatcg acggcgtggg cgagctcggt
     1621 ctgcgagatg cttcgctcgg cgcgggcgac ccggagccgg ttgatgagac gcgggccacg
     1681 tacggaagcc atacccggat ggtgtggcgg agcgggtgcc gttgccaagg gtccttggca
     1741 atctgccaag ggtgccagac ggcaggtgtg gtcaattcgc gccgtccggt tgtgacagca
     1801 gagctggcac ggtaaggcga gagagttccc gacatggaag cccgcatcgt ctctttccca
     1861 agcccgctgg cgggcgttcg tgtcgccggg gaccgcatcg acgtggaccg cgtgtcgctg
     1921 accgatggtc cgctcgccgc gtggctcgcc gcacagccgg cggatgacca tgcggtgctc
     1981 atcgaacgcg cgattcgcat cgggctgacc gcgctccaga gcgtcggcgt gacgctgaac
     2041 gtggatgccg tgcgggccga gttcgacagc ttcgtgctcc agaacaacgc cgcgaacgag
     2101 cgcgctgcgg aggcgctgga gggcgtcctc cgggcgaact tcgcggacgg cgatgggcgg
     2161 ctgccgcgca cgctcgagac gttcctcggt gaccgcggca agctgcagtc gatggtcaac
     2221 gagctgttcg acccgaatcg gcgcgattcg gccatcgggc gtatctcggg gatgctggac
     2281 acgtacttcg acggcgacgc ctcgcgcctg gcgcgattgc tggacccgac gcgactcggc
     2341 tcgccgctgc accagttccg ctccgaggtc gcggacgggt tcaaggcgct ccacgagaag
     2401 ctcgttgcca tcgaggccgc gcagaaggcg cgcagccagg agcggtcgaa gtccgctgcg
     2461 aagggcggcg acttcgagga cctgctcgag ggcatgctcg gcgacatcgc gcgtggctcg
     2521 agcctcctgc tcgagcgaac gggcaccgac gccggcgacg cgggtcgctc caagaagggc
     2581 gacttcgtcc tgacgctgga cccggacgtg tgccgcggcg ccgacgtccg catcgtcgtc
     2641 gaggcgaagg accgcaagat gtccggccgc gagatgcgcg acgagctccg ggcggcaaag
     2701 acgaaccgtg atgccgccgc agcactggtg gtgttcacgc cggcgcatgc tccctccggg
     2761 attgcgccgt tcgatgtccg cgccggcgac gtctacgcgg tcatcgaccc ggccgacccg
     2821 gatttcgcaa ccctcgaggc cgcggtgcgg ctggcgcggc tcgttgccgt cgccacgctg
     2881 cgggagaacg aggccgaggt cgacctcggc gaggtccagg cggccctcac cggcatccgc
     2941 gagacgctcg acctggtccg cggcctgaag gtccagctga ccagcatcgg caccacgtcc
     3001 gaggcagtct cgaaggcgct cgacaagctc agggaagcag tcatcgcatg gatttcgcga
     3061 gccgaacggg agctcgcaac ggcgccttga tccgtgccgc gcgcggcgtt gtcgcctccg
     3121 ccgactcgct cacgcactga agtgcgctct ctcgtcgtac tcggcgccgc cttgcgcgcg
     3181 acccggctga agacgccgtt gggccagatg gcaccagtcg ttggtcatct gaccctcggc
     3241 cgcctgtcgc ggtacgaacg gctgactggt gatgcgcaga caggcgcggc attgtccggg
     3301 tatgaagaag cgcgtagccg cggcgatcct gtgggcagcg gtcggatggg tcgggggcga
     3361 gctggtgacc ggcatcttcg gtgccccgtc cttcatcgcc ctgatcccgg cggtcctgat
     3421 gccggcgttc gtgctctggg accccacggg cgcgctgtgg ggccgatcgt tcaccggcaa
     3481 gctgatcgtc cggccgatca acgagctcgc ggacgagctt gatgcccgga acgcaccccc
     3541 accgtccgcc gaggcagaac gcatctcccg ctgatcgagc ctctcgtcgc gcactcgcaa
     3601 gggtgggcgc gacgatcgca ggcggagcga ttgcagagcg gtggtagagt cggctcccgc
     3661 tcgccgcgag caggcgagcc gttcgagcat gagggagacc cgtggcgcgc tccatgtgga
     3721 agggggccat ccaattcggc ctcgtgacca tcccggtgaa gctgtacatc gcgactgaat
     3781 cgcgcgccgg gatcagcttc aacatgctcc atgaagagga cctgtcccga atccagatga
     3841 agatcttctg cccggtcgag gaggagatca tcgatcggaa ggacacggtc aagggctacg
     3901 agtactcccc ggacaagtac gtcgtcatcg acgacgagga cctcgagaag ctgccgctca
     3961 agacggtccg ctcgatcgag atcgagcagt tcgtggaccg cagcgaggcc gagacccaca
     4021 cgaagttcgt caagcaggcg tactacatcg agcctgaccg ggtcggacgc aaggcgtttg
     4081 ccctgctcaa ggacgtgctc gcggacaagg gcctcaccgc gatctgcaag atcgtgatcc
     4141 gcgatcgcga ggcgctcgcg gccctcgacc cgttcgagaa cacgatgctc ctctcgaccc
     4201 tctattggcc cgacgagatc cgcgcgctcg gcgatctcga cctgcccgac gaggagcccg
     4261 agatcaagcc cgccgagaag aagatggccg cccagctgat cgaggccatg accggcgaat
     4321 tcgacccgac gcaataccgc gatgagtatc gcgaagcgct gatgcaggtc atcgagtcga
     4381 agatcgaggg tcgtgagacc gtggagatcg agccggcggc cgagccgacc aagctcgtcg
     4441 acctgatggc cgcgctccag gcgagcgtca acgctgcgac gaccgctcgc gagtcgaagg
     4501 gcggcaagcc ggtttccgtt gccgaggcca aggagcagcg cgccgcgaag ggcaaggcca
     4561 cggtaaaggc gtcctcgagc aagggaaagg ccgacgccga ggacgagacc gaagagcgac
     4621 ccgcgcgacg ccgcaagagc gcctgaccgc gcaaacagcg cctcacccgg gcgggcgcaa
     4681 gcgcgcctga gcgactgtcg cgccgcctgg aggcgcgcct accgacccgc gaggatgcga
     4741 tccgcgtcc
//
LOCUS       DATBSW010000305         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_910606, whole genome shotgun sequence.
ACCESSION   DATBSW010000305 DATBSW010000000
VERSION     DATBSW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910606"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VIF63_08430"
     CDS             complement(<1..1144)
                     /locus_tag="VIF63_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8239447.1"
                     /translation="MRHRFLSISFAMAFVVAACNSGASPSPVQTGPGATTAPPATTTA
                     AFDPMSVSGDITLGAWESSPAEGTALKAALDAFAVAYPNIKVKQETVAGDYRVQMTTN
                     FSAGTVQDLFYVNAEYTPEWIEEGFLEPLDPFIAKQGFDTSKFFQGYLDTFKGPDGKQ
                     YGLPKDGNTIAMGYNTDLVTTPPTTLAELVTAADDLKGKGDLKAPICINPGLDRGLAF
                     IYAEGGSLLTADNSAEAIDTPASKAAVQWYLDLVKNGQAMTAAQLGDGWCGEALGKGD
                     VAFAFEGGWLKGALDNDFPDTNWAFAEMPAGTAGKATISYTAAYAINPDSENKDAAWV
                     VLQYLTGPEGMAKWTAGGIAIPSRSDVPTPAGFEVIVAGGAYAKPGS"
     gene            complement(1426..2331)
                     /locus_tag="VIF63_08435"
     CDS             complement(1426..2331)
                     /locus_tag="VIF63_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8239448.1"
                     /translation="MRVFRKLALGASVLALALSACSTGGGGATIKIGSDGFDEARVVA
                     EIYAQALEAKGFTVNRDGIGLGTRAVTAAALEKGDINLKPEYLGGGLAYYKGTPGQSS
                     ADNAAGLQDKLAAIGGGITVLDYTPAQDTNAFVVRKETADQYHLVKMSDTAAVQDQLK
                     WALATDCPTNPVCAAALKSAYGIDQPDATLLGPCSGPMADALLNKTVDVAELCSTGPE
                     IITNGWVVLEDDKQTQPAENIAPIVRNDLVKSLDAAKFNATLNEVSAKIDTKTLAQLY
                     YDVAVGHKDLKVVATAWLKAVGIVT"
BASE COUNT          364 a          789 c          868 g          445 t
ORIGIN      
        1 cggagcccgg cttggcatac gcgccgccgg cgacgatcac ttcaaagccg gccggtgtcg
       61 ggacatcgct tcgggacggg atggcgatgc cgcctgccgt ccacttggcc atgccctcgg
      121 ggccggtcag gtactggagg acgacccagg cggcgtcctt gttctcgctg tcagggttga
      181 tcgcgtacgc ggcggtgtag ctgatggtcg ccttgccggc ggtgccggca ggcatctccg
      241 cgaacgccca gttggtgtcc gggaagtcgt tgtccagggc gcccttgagc cagccgcctt
      301 cgaaggcgaa cgcgacgtcg cccttgccga gcgcctcgcc gcaccagcca tcgcccagct
      361 gcgccgcggt catggcctgg ccgttcttga cgaggtcgag gtaccactgg acggcggcct
      421 tggacgccgg cgtatcgatt gcctcggcgg agttgtccgc ggtgaggagc gagccgccct
      481 cggcgtagat gaacgccagg ccgcgatcga ggcccggatt gatgcagatc ggagccttga
      541 ggtcgccctt gcccttgagg tcgtcagcgg cggtcacgag ctcggcgagc gtcgtcgggg
      601 gcgtcgtcac caggtcggtg ttgtagccca tggcgatcgt gttgccgtcc ttcggcagtc
      661 cgtactgctt cccgtcgggg cccttgaacg tgtcgaggta gccctggaag aacttcgagg
      721 tgtcgaaacc ctgcttggcg atgaacggat cgagcggctc gaggaagcct tcttcgatcc
      781 actccggcgt gtattccgcg ttgacgtaga acaggtcctg cacggtgccg gcgctgaagt
      841 tcgtggtcat ctggacgcgg tagtcgcctg cgaccgtttc ctgcttgacc ttgatgttgg
      901 gatacgcgac ggcgaaggcg tcgagcgctg ccttgagggc cgtgccctcg gccggcgacg
      961 actcccacgc cccgagcgtg atgtcgccgc tgacgctcat gggatcgaac gccgctgtgg
     1021 tcgtcgcggg cggtgcagtg gtcgcacccg ggccggtctg gaccggcgac ggcgacgcgc
     1081 cgctgttaca ggccgcgacg acgaatgcca tcgcgaacga gatggaaagg aatctgtggc
     1141 gcatgtgttg gggcctcccc gagggaaaat gacccgactc gagctcgcgg agcagccgga
     1201 tgggtgcccc gagcggtcag gtattcccga ggatggggcc cagcgcattg caagcccgtc
     1261 aaacgaacgg ccaaagggac acgtttgcgg accaactcca ttgcaaacat gacccgaacg
     1321 ttcgggcggc tcgatcgcgg acccggaacg cgacgagccc ccggcgatgc cgggggctcg
     1381 ggtcgagtgc ggtggtatgg cggcgggacg ccgccgagag gttgcctagg tgacgatccc
     1441 cacggccttc agccaggccg tcgcgacgac cttgaggtcc ttgtgaccca cggcgacgtc
     1501 gtaatacagc tgggcgaggg tcttggtgtc gatcttggcc gacacctcgt tgagggtcgc
     1561 gttgaacttc gcggcgtcga gcgacttgac gaggtcattg cgaacgatcg gcgcgatgtt
     1621 ctcggccggc tgcgtctgct tgtcgtcctc gaggacgacc cagccgttcg tgatgatctc
     1681 gggaccggtc gagcacagct cggcgacgtc gacggtcttg ttgaggagcg catcggccat
     1741 cgggccgctg cacgggccga gcaacgtggc gtcgggctgg tcgatgccgt aggccgactt
     1801 gagcgccgca gcgcagaccg ggttcgtcgg gcagtccgtc gcgagcgccc acttgagctg
     1861 gtcctgcacg gccgcggtgt cgctcatctt gacgaggtgg tactggtctg ccgtctcctt
     1921 gcgaacgacg aaggcgttcg tgtcctgggc cggcgtgtag tccagcacgg tgatcccgcc
     1981 gccgatcgcg gcgagcttgt cctggaggcc agccgcgttg tcggcggagc tctggcccgg
     2041 cgtgcccttg tagtaggcga ggccgccgcc caggtactcg ggcttcaggt tgatatcgcc
     2101 cttctccagc gccgcggcag tcacggcacg tgtgccgagg ccgatgccgt cccggttcac
     2161 ggtgaagccc ttggcctcga gggcctgggc atagatttcg gcaacgacgc gagcttcatc
     2221 gaatccatcg gacccgatct tgatggtcgc gccgccccct cccgtcgagc acgcgctgag
     2281 tgccagcgcg agcacggacg ccccgagggc aagcttgcgg aatacccgca tgggtctggt
     2341 tcctcctcat caacggccgc cggtccatcc gggcggtcgc gcaaccatat caggccttca
     2401 agccggttac tttttcgtgt ctgggaacac ccgaggtcgc cggcagccgc ccgagcggca
     2461 ggcaca
//
LOCUS       DATBSW010000306         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_982403, whole genome shotgun sequence.
ACCESSION   DATBSW010000306 DATBSW010000000
VERSION     DATBSW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982403"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VIF63_08440"
     CDS             <1..180
                     /locus_tag="VIF63_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239449.1"
                     /translation="PRDGVDTVWPGKGVIYSQRLSAERTRSRLNRIVGTPAYRSMTIR
                     SWQTTLALLDLMRKR"
     gene            216..884
                     /locus_tag="VIF63_08445"
     CDS             216..884
                     /locus_tag="VIF63_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239450.1"
                     /translation="MDESASFETRAVLAPRRARVARLALLVPVVALVAIAWAGLNGPS
                     SDSTAAASPGPNTPQPTTVSVPAVVPAPPVEAAYPAKVVGIDVQKLGDLQLGGIGFDE
                     IVAIAGWYVPTAITDCPALAAIYRDGALPYLRGDRDKLAFCVRSGVLYPSRPDLANVA
                     ATGVAVTVVVGVVMPLELEIVDAQATQVVVLGRFSKSSDGCRVGIGCSRELLVDHVGW
                     TPGA"
     gene            complement(925..2595)
                     /locus_tag="VIF63_08450"
     CDS             complement(925..2595)
                     /locus_tag="VIF63_08450"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239451.1"
                     /translation="MAGLGVYRQLLANRLLTRFLFGQFISGIGDWLYIVAIFVLIYRE
                     TGDAALVGLFGAVRVIPYVVLSIPAGLIADRFDRRLVLLVSDLWRGAMMVLLTILVIV
                     EAPVLPIAVVAILATCGSAFWYPAFGAYLPNLVTDEQQLGPANSAWSSLQNLSFIIGP
                     AVGGLLLAFGGVTVAFILNALTFVVIAFILWSLPPARPRPSVVEATPTAETPESAETP
                     AAPLAGPKVAPRPMAGLLIIQAMAGFFDSGMQVLTVILAINVLNAGEAANGYLNAAIG
                     VGGLVGAIASGVLVLRRGLGMPLVVGGVVFAIGLVVLGLVPNLAVAIVALIIAYAGAI
                     LLDVVMTTLFQRVVPDERRGRWNGIKESVFTLSGAGGAIALPFLVVNVGAAPTLGVAA
                     AVIVVATVAGRALIGGAATRAPSPFEATLARVAELPLFTGVPSSRLEAALAHIREVPV
                     RAGEVVIRQGDPADRFYMIESGSFVVTQAPAPGAAQVVLQHLGPNEVFGELGLLRGAP
                     RRATVTAETDSVLLALEGRDFLKLVGAGGPLRGRLLGLYTGGGSESNS"
     gene            2710..3744
                     /locus_tag="VIF63_08455"
     CDS             2710..3744
                     /locus_tag="VIF63_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239452.1"
                     /translation="MGFLDDLRNDPPAEGGLDAVPEPVGTTPQRQKVVARLRWAPART
                     ESDAPTALRSWPVAVALAAAIVVTAIVVGSGPGQIRTWPFEGMWTSLDPTDGSTQTLV
                     VGPGDTPPVRFEDDFGVDGLCVEDPTAFIAEGAGAIRGNHLDVSFPDGGGCVGVTAPV
                     ADMAFDLEATTGELLGADGRTWSLLTDATAEPTAPAATDGPATDSPATDAPSATGVQA
                     TSAAVVTDAIATQGVLDPSMPTGATIRIRYMPTPATTIQATRFQIAVPADFDVRACDA
                     GTLKLWRTTGNPIAPDVTIAPGQVMTIWVLNVDNQPLVFTAAVWPDEMTPALRDFLNS
                     FREGLFLLPR"
BASE COUNT          598 a         1317 c         1361 g          538 t
ORIGIN      
        1 ccgcgcgacg gcgtcgacac ggtctggccg ggcaagggcg tgatctactc gcagcgcctg
       61 agtgcggagc ggaccaggag ccgcctcaac cggatcgtgg ggacgcccgc ctatcgatcg
      121 atgacgatcc gcagctggca gacgacgctc gcgctgctcg acctgatgcg gaagcgctag
      181 cgcgcgcttc ggttgctggg catacgctcc acgagatgga cgagtcggca agcttcgaga
      241 ctcgtgcggt cctcgcgccc aggcgcgccc gcgtggcccg gctcgcgctc ctggtgccgg
      301 tggtcgcgct cgtcgccatc gcctgggcgg gcttgaacgg accgagctcg gattcgacgg
      361 ctgcagcgtc gcccggtccg aatactcccc agccgacgac ggtgtccgtg cccgccgtcg
      421 tgcccgcgcc accggtcgag gcggcctatc cggcgaaggt cgtcggcatc gacgtccaga
      481 agctcggcga cctgcagctt ggtgggatcg gcttcgacga gatcgttgcc attgccggct
      541 ggtacgtccc gacggcgatc accgactgtc cagcgctcgc cgccatctac cgggacgggg
      601 cgctgccata cctccgcggc gacagggaca agttggcgtt ctgcgtgcgc tccggggtgc
      661 tctatccctc gcggccggac ctcgcgaacg tcgctgccac gggcgtcgcc gttacggtcg
      721 tcgtgggcgt cgtcatgccc ttggagctcg aaattgtcga cgcgcaagcg acgcaggtgg
      781 tcgtcctcgg gcgtttcagc aagtcgagtg acggatgccg cgtcgggatt ggctgcagcc
      841 gtgagctcct ggtcgaccat gtggggtgga cgcccggcgc ctgaccccgc tggctcgtgc
      901 ccttgatggg cacgcgcggc gttgctagct gttggattcc gagccgccgc ccgtatagag
      961 gccgagcaac cgccctcgga gcgggccgcc ggcgccgacc agcttcagga agtcgcggcc
     1021 ctcgagggcg agcagcacgc tgtcggtctc ggccgtgacc gtcgcccgcc gcggcgcacc
     1081 gcggagcaga ccgagctcgc cgaacacctc gttcgggccg agatgttgca gcacgacctg
     1141 cgccgcgccg ggtgcgggcg cctgggtcac gacgaacgag ccggactcga tcatgtagaa
     1201 gcgatcagcc ggatctccct ggcggatgac gacttcgccg gccctgaccg ggacctcgcg
     1261 aatgtgcgcc agggcagctt cgaggcggga cgatgggacg ccggtgaaca acggcagctc
     1321 ggccactcgg gcgagggtcg cctcgaacgg gctgggcgca cgggtcgcgg caccgccgat
     1381 gagggcgcgc ccggcgaccg ttgccacgac gatcaccgcc gccgctacac cgagcgtcgg
     1441 tgccgctccg acgttgacga cgaggaaggg gagtgcaatg gccccgccgg cgccggacag
     1501 cgtgaagacg gattccttga tgccgttcca gcgcccgcgc cgctcgtccg gaacgacccg
     1561 ctggaacagg gtcgtcatca cgacgtcgag gaggatcgcg ccggcgtagg cgatgatcag
     1621 cgcgacgatc gcgacggcga ggttcgggac gagtcccagg acgacgaggc cgatcgcaaa
     1681 cacgaccccg cccacgacca ggggcatccc gaggccgcgt cgcaggacga gcacgcccga
     1741 tgcaatggcg ccgaccaggc caccgacgcc gatcgccgcg ttcaggtagc cgttcgcggc
     1801 ttcacctgcg ttgaggacgt tgattgcgag gatgacggtc agcacctgca tcccgctgtc
     1861 gaagaagccg gccatggcct ggatgatcag caggccggcc atgggccgcg gcgccacctt
     1921 cgggccggcc agcggtgccg ccggcgtctc ggccgattcc ggcgtttcgg ccgtcggcgt
     1981 ggcttcgacg acggacgggc gcggccttgc cggcggcagc gaccacagga tgaacgcgat
     2041 caccacgaag gtgagcgcgt tcaggatgaa tgccaccgtc accccgccga acgcgagcag
     2101 cagcccgccg acggccggtc cgatgatgaa gctcaggttc tggaggctcg accaggcact
     2161 gttcgccggc ccgagctgct gttcgtccgt gaccaggttc ggcaggtacg caccgaaggc
     2221 ggggtaccag aacgccgacc cgcacgtcgc caggattgcc acgaccgcga tcggcaggac
     2281 gggtgcctcg acgatcacga ggatcgtcaa caggaccatc atcgcgcccc gccagaggtc
     2341 agagacgagc agcaccaggc ggcggtcgaa ccggtcggcg atcaggccgg ccgggatcga
     2401 gagcacgacg tacgggatga cgcggaccgc cccgaagagc ccgaccagcg ccgcgtcgcc
     2461 ggtctcgcgg tagatcagga cgaagatcgc gacgatgtac agccagtcgc cgatgccgct
     2521 gatgaactgg ccgaacagga atcgcgtgag cagccggttc gccagcagct gccggtacac
     2581 accgaggccc gccaagcgca tcgccctcct gcgagggaca ggctagtcgc acgaatggcg
     2641 cggtgggcgg cgattcggcc tgccacgcct ggcgcgggga tctgcgtgtc gttcccgtag
     2701 tctttccggg tggggttcct ggacgacctt cgcaacgatc caccggcaga gggcggactg
     2761 gacgccgtcc ccgagccggt cgggacgacg ccgcagcgcc agaaggtcgt ggcgcggctc
     2821 aggtgggcgc cggctcggac cgagtcggat gcccccacgg cgctccgttc ctggcccgtc
     2881 gccgtggcgc tcgccgccgc gatcgtggtc acggcgatcg tggttggaag cgggcccggc
     2941 caaatcagga cctggccgtt cgagggcatg tggacatcgc tcgacccgac cgacggcagc
     3001 acccagacac tcgtcgtggg tccgggcgat acgccgcccg tccgcttcga ggacgacttc
     3061 ggggttgacg gcctgtgcgt cgaggacccc acagcgttca tcgccgaagg tgccggcgcg
     3121 atccggggca accatctcga cgtgagcttc ccggacggcg gcggttgcgt cggcgtgaca
     3181 gctccagtgg ccgacatggc gttcgacctc gaggcgacga cgggcgagct gctgggcgcg
     3241 gatgggcgga cctggagcct cctgacggac gcgactgccg agccgacggc gccggccgcg
     3301 actgacggcc ccgcgacgga cagcccggcg accgatgccc cgagcgcgac gggcgtccag
     3361 gcgacgtcgg cggcggtcgt gacggatgcg atcgcgacgc aaggcgtcct cgatccatcc
     3421 atgccgacgg gcgcgacgat ccgcatccga tacatgccca ccccggcgac gaccatccag
     3481 gcaacgaggt tccagatcgc ggtgccggcg gatttcgacg tgcgtgcgtg cgacgccggg
     3541 acgctcaagc tctggcgtac gaccggcaac cccatcgcgc cggacgtcac gatcgcgccg
     3601 ggtcaggtga tgaccatctg ggtcctgaac gtagacaacc agcccttggt gttcacggcg
     3661 gccgtctggc cggacgagat gaccccggcc ctgcgcgatt tcctcaacag tttcagggaa
     3721 ggcctgttcc tgctcccgcg gtaacgcgct atcggcccag cgcgttgggc gcgccggttc
     3781 gcggtgaggt cgcagtcacc agcaggccag tccc
//
LOCUS       DATBSW010000307         7970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_241160, whole genome shotgun sequence.
ACCESSION   DATBSW010000307 DATBSW010000000
VERSION     DATBSW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241160"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /gene="thrS"
                     /locus_tag="VIF63_08460"
     CDS             <1..1616
                     /gene="thrS"
                     /locus_tag="VIF63_08460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY8239453.1"
                     /translation="VPRALTPDDLEAIEGRMRESVAANHRFVRKEVEFDDGRAAVEGQ
                     GQLYKVEILDDLASKARAAGEGSPAITFYEHGPFSDLCRGPHVASTGKIGPFKLLSVA
                     GAYWRGDSKRPALQRIYGTVWADQEALDAFLWRRAEARKRDHRKLGVQLDLFSFHDVS
                     PGAPFFHPKGQLLWRMLESSMRELQARRGYLEVATPIVVSERLWRQSGHWDLYKEHMF
                     LVESENQTFSLKPMNCPESTFIYRSRKRSYRDLPLRYAEYGRLHRNELSGALSGLTRV
                     RQFVQDDAHIYVRPDQLMEEIQALLGEVREAYAWFGLTPRFSFATKPDKAIGDPALWE
                     RAERLTMEAFAASDISYELKPKDGTFYAPKIDIYIDDALGREWQMATIQIDLTMLPER
                     FDLTYDDESGAGVRPIAIHRAIYGSLERFIGIIIEHFAGAFPLWIAPVQAAIIPIADR
                     HIEAAEELASVLRARGLRVEVDGSNNRMQNKIRLAQEQKVPYMLVLGDREIEARTAAP
                     RTRAGEQQAPEAWDALADRLAAEAAARGA"
     gene            complement(1618..2115)
                     /locus_tag="VIF63_08465"
     CDS             complement(1618..2115)
                     /locus_tag="VIF63_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239454.1"
                     /translation="MYRTRPRRLAATLLASSLLLVSAGSVAAADPPGQYVLDVFSCGM
                     TDLEGNFLDSNAVPAGSEVIVFEGWFAKTRGQLQSFLNNVTWVLTINGQAADVRPYLS
                     GLLNFGPFWGDLWSFSAGTLDAGQELHTHYDNVLKSASFDGDVHWRKGSLFNGGVDCT
                     VTVAP"
     gene            complement(2134..2790)
                     /locus_tag="VIF63_08470"
     CDS             complement(2134..2790)
                     /locus_tag="VIF63_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239455.1"
                     /translation="MHRLRNAALAAIMLALVAGSTAAAAGPRLRGVLPPTAHPRGHSL
                     TELATAWNMWAFGSSTDNPLLAVRCEQSPIDPKIWFLPVSLGGEWENTCDVPQGAFLL
                     MNPGGSECSNLEAEPYFGTDEADLRSCVNETFVDLTYLEVTVNGTTVTNLDPWIVVTD
                     MINLPADNLFSASPGISMDKGYFLVLQPMSRGTHTLRAYDEFFDGGFQAGITYTINVH
                     "
     gene            2931..5702
                     /locus_tag="VIF63_08475"
     CDS             2931..5702
                     /locus_tag="VIF63_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY8239456.1"
                     /translation="MRFRLLGTLEVIADDGTPVAIPARKRRALLAVLLLRRGRSIQRE
                     ALIDALWGEAAPDAASESLFAHVSRLRREIGATAIQRVPGGYLLPVEDDQLDVLAFER
                     EVAAGRQELALRRWHAATATLGAALERWQGPALAEFSSEPFAQPEIVRLEELRIGALE
                     DRIDADLAIRREHDVVPELEQLVQEHPLRERLWGQLLVALYRSGRQSEALERYRELRR
                     TLLAQLGLDPSPELQELQRRILVQDPGLDVTEPSERQAAPDLPAYPTRTVGRRREIEI
                     ARATLSSSRLLTLTGPGGVGKTRLAVLVAETLLSEYREDIRFVDLSMVRDPEQVLERI
                     GALVGGGGRPEEAIGERRMLLVLDNFEQVLDASASISHLIGRCPGLRVIITSRAPLRI
                     AAEQQLDVPPLDSADSAELFIDRAAAAVAAEALPTEAVREVVARLDGLPLALELAAAR
                     VKVLTISAIRDFLADRMSFLTAGQRDAPDRHRTLRDTIRWSYELLAPEARAVFRKLSV
                     LAPGFDVEAAFAVGEAGLDEVAELVDHSLLRRVEDRYAMLETIRAFAEGAASGPEATA
                     AHERHLRHFTALPLAARDRVAQLAGSLQQGADEWLDVCASNQENLRLAFEWARATGDA
                     QAMVSLLRGVGAYWAYLGTTEEALRWAEMTLERSEGLPPADLERVRVGAAEFARFSDQ
                     LPLALSLLQSVLDGATERADLVRIANTLDDLANTYASMDRETEALALLGRAHETHRQE
                     PNCDPRHLGHTLEVEMFVLLRLGRVDEAEETSKAYVEVNSTRPGWTLRNLESELVLAA
                     LAAAAGRRYDARAGYGRVVVAASRGQFRGMIADALDGLAGIDRDERPGDAAMLLGMAD
                     RARAESRSTVFFAPQRRALIDGLRETLGPDRYEAARSAGEGVPVIEMAAAALPSKPEL
                     VAEPAARST"
     gene            5827..6591
                     /gene="infC"
                     /locus_tag="VIF63_08480"
     CDS             5827..6591
                     /gene="infC"
                     /locus_tag="VIF63_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY8239457.1"
                     /translation="MNEMIRIPQVRVIDDEGNQLGVMPTPRALEMAQEKGLDLVEVAP
                     MAAPPVVRFLDFGQYKYELTKREKEAKRRQRIGFKEVRLSPKIGVGDFDTKLHRAVEF
                     LEDGDRIKVTVRFRGRELSHPELGRVLLERFSKQVEEHAIIERQPVLEGKSMFIVLAS
                     THKPKVVEHEPKHRDEQHDAEPAPAESAPVAAPAAEAAAAPKPAAAPKPAATPVAAAK
                     AAPAAKPEPAAATEPATAKAPTNGAAAPAAVSTTGD"
     gene            6600..6797
                     /gene="rpmI"
                     /locus_tag="VIF63_08485"
     CDS             6600..6797
                     /gene="rpmI"
                     /locus_tag="VIF63_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY8239458.1"
                     /translation="MPKMKSHKAAQKRIGVTGSGKVKRVNAWRGHHLEIKSSRRTRRY
                     AGISTMNKTHAKQVKRLLPYL"
     gene            6813..7163
                     /gene="rplT"
                     /locus_tag="VIF63_08490"
     CDS             6813..7163
                     /gene="rplT"
                     /locus_tag="VIF63_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY8239459.1"
                     /translation="MSRVKRGVTAHARHKRLLQDAEGRKGTRSRLVKPAREALLHALA
                     YATRDRKQRKRDFRGLWIVRINAAVRSQGVTYGRFIQGLKKADIQIDRKILADIAVRD
                     AGTFTQIVEAAKGS"
     gene            7274..>7970
                     /locus_tag="VIF63_08495"
     CDS             7274..>7970
                     /locus_tag="VIF63_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY8239460.1"
                     /translation="MDLAQLTSDLDALAAETAAALAAASDVAAIEALELDVLGKKGRL
                     TGVLRGIGALPADDRPKVGAVANRVRSTIEAAIAERRLVLGSAELDARLLAETIDVTM
                     PGRPIRRGSLHPSIESMTEIARIFGQFGFQVYETPEIEDDQANFQDLNIPPDHPARDL
                     WDTLYVDIPGRLLRTHTSPGQIHVMRTVKPPIRALPPGRCFRYEAIDASHGSEFFQVE
                     GLMIDEGTTMGDLR"
BASE COUNT         1342 a         2723 c         2741 g         1164 t
ORIGIN      
        1 acgtgccccg cgcgctcacg cccgacgacc tcgaggcgat cgaaggccgg atgcgcgaga
       61 gcgtcgccgc gaaccaccga ttcgtccgaa aggaggtcga gttcgacgac ggccgcgccg
      121 ccgtcgaggg ccagggccag ctctacaagg tcgagatcct cgacgacctc gcgtcgaagg
      181 cgcgggcggc cggggagggg agcccggcga tcactttcta cgagcacggg ccgttcagcg
      241 acctgtgtcg cgggccgcac gtcgcctcga cgggcaaaat cgggccgttc aagctgctgt
      301 ccgtcgccgg tgcgtactgg cgtggcgact cgaagcggcc ggcgctgcag cgcatctatg
      361 gcacggtctg ggccgaccag gaggcgctcg acgcgttcct ctggcgacgg gccgaggcgc
      421 gcaagcgcga tcaccgcaag ctcggggtcc agctcgacct gttcagcttc cacgatgtca
      481 gcccgggcgc gccgttcttc catccgaagg gccagctgct ctggcgcatg ctcgagagct
      541 cgatgcgcga gctccaggcg cggcgcggct acctcgaggt cgcgacgccg atcgtggtga
      601 gcgagcggct ctggcgccag tccggccact gggacctcta caaggagcac atgttcctcg
      661 tcgagtccga gaaccagacg ttcagcctga agccgatgaa ctgtcccgaa tcgacgttca
      721 tctaccgatc gaggaagcgc tcgtatcggg acctgccgct gcgctacgcc gagtacggcc
      781 ggctgcacag gaacgagctc tcgggcgccc tgagcgggct gacgcgcgtc cgccagttcg
      841 tccaggacga cgcgcacatc tacgtccgcc cggaccagct catggaggag atccaggcgc
      901 tcctgggcga ggtccgcgag gcgtatgcct ggttcggact cacgccgcgg ttcagcttcg
      961 cgacgaagcc ggacaaggcc atcggcgacc cggcgctgtg ggagcgcgcc gagcggctga
     1021 cgatggaggc ctttgccgca tcggatatca gctacgagct gaagcccaag gacggcacct
     1081 tctacgcgcc caagatcgac atctacatcg acgacgccct cggccgcgag tggcagatgg
     1141 cgacgatcca gatcgacctg acgatgctgc ccgagcggtt cgacctgacg tacgacgacg
     1201 agtcgggcgc cggcgttcgc ccgatcgcga tccaccgcgc gatctacggc agcctcgagc
     1261 ggttcatcgg catcatcatc gagcacttcg cgggcgcgtt ccccttgtgg atcgcaccgg
     1321 tccaggccgc gatcatcccg atcgcggacc gccacatcga ggccgcggag gagctcgcct
     1381 ccgtgctccg cgctcggggc cttcgggtcg aggtcgacgg ctcgaacaac cgcatgcaga
     1441 acaagatccg cctcgcccag gagcagaagg tgccgtacat gctcgtgctc ggcgaccgcg
     1501 agatcgaggc gagaacggct gctccccgga cgagagccgg ggagcagcag gcgccggaag
     1561 cgtgggatgc cctggccgac cgcctcgccg cggaggccgc cgcccgaggg gcctagatca
     1621 cggcgcgacg gtgacggtgc aatcgacacc gccgttgaag agcgacccct tccgccagtg
     1681 gacgtccccg tcgaacgagg ccgacttcaa cacgttgtcg tagtgcgtgt ggagctcctg
     1741 gccagcgtcg agcgtgccgg cggagaagga ccacaggtcg ccccagaacg ggccgaagtt
     1801 gagcagccca ctgagatacg gcctgacgtc tgcggcctgc ccgttgatcg tcaggaccca
     1861 ggtgacgttg ttgaggaagg actgcagctg ccctcgagtc ttcgcgaacc acccttcgaa
     1921 gacgatgacc tcgctcccgg ccggcaccgc gttcgagtcg aggaagttgc cctccagatc
     1981 tgtcatgccg cacgagaaga cgtcgaggac gtactggccc ggcggatccg ccgcggcgac
     2041 gctgcctgcc gatacgagca ggaggctcga cgcgagcagg gttgcagcga ggcggcgtgg
     2101 acgtgttcga tacatcggtc gtctccgttc ctgctaatgg acgttgatgg tgtaggtgat
     2161 cccggcctgg aagccaccgt cgaagaactc gtcgtaggcc cgcagcgtgt gcgtgccccg
     2221 gctcatcggc tgcaggacga ggaagtagcc cttgtccatc gagatgcccg gggacgcgct
     2281 gaacaggttg tcggcgggca ggttgatcat gtcggtcacg acgatccagg gatcgaggtt
     2341 cgtgacggtc gtgccgttga ccgtgacctc gaggtaggtc aggtcgacga aggtctcgtt
     2401 gacgcaggac cggaggtcgg cctcgtcggt gccgaagtac ggctcggcct cgaggttgga
     2461 gcactcggag ccgccgggat tcatgagcag gaacgcgccc tggggcacgt cgcaggtgtt
     2521 ctcccactcg cccccgagcg agaccggcag gaaccagatc ttcggatcga tcgggctctg
     2581 ctcgcagcgg accgccagga gcgggttgtc ggtgctcgag ccgaacgccc acatgttcca
     2641 ggcagttgcg agctctgtca gggagtggcc acgcgggtgg gcggtcggcg gcaggacgcc
     2701 acgcagcctt ggtcccgcgg ccgcggcggt cgatcccgcg acgagcgcga gcatgatcgc
     2761 ggcgagcgcc gcattccgca gtcggtgcat cggtcgatcc tccttgtggg ccgacaaccg
     2821 gcccgtctgc gcgacgatcg gcgaggcacc ttgccgatcg cttgcggcgc ccgcttgcga
     2881 cgcggaagtc ggcgagcgga tacttcgtcg aatccacgta tgagggtgcg atgcgctttc
     2941 gcctcctcgg gacgctcgag gtcatcgccg acgacggaac gccggtggca attccggccc
     3001 gaaagcgacg agcgttgctg gccgtcctgc tcctccgccg cggtcggtca atccaacgcg
     3061 aggcgctgat cgacgcgctg tggggcgaag ccgcgccgga tgcggcatcg gagtcgctgt
     3121 tcgcccacgt ctctcgtctc cgacgggaga tcggcgcaac ggcaatccag cgtgttcccg
     3181 ggggctacct gctgccggtc gaggacgacc agctggacgt cctcgcattc gaacgcgaag
     3241 tagcggcggg ccgtcaggag ttggccctgc gccgctggca cgctgcgacg gcgacgctcg
     3301 gcgcggcact cgagcggtgg cagggaccgg cccttgccga attctcgtcc gagccatttg
     3361 cgcagcccga gattgtccgc ctcgaggagc tgcgtatcgg cgcgctcgag gaccgcatcg
     3421 acgccgacct tgcgatacgc cgggagcacg acgtcgtccc cgagctcgag cagctcgtgc
     3481 aggagcatcc cctccgcgaa cgtctctggg gacagctgtt ggtcgcgcta tatcgcagcg
     3541 gccgccaatc cgaggcactc gagcgctacc gcgagctccg tcgcaccttg ctggcacagc
     3601 tcggcctcga cccgagcccg gagctccagg agctgcaacg ccgcattctc gtgcaggatc
     3661 ccgggctcga cgtaacggag ccttcggagc ggcaagctgc ccccgacctg ccggcgtacc
     3721 cgacgcgaac ggtgggccga cggcgtgaga tcgagatcgc ccgagcgacg ctgtcctcct
     3781 cccgcttgct gacgctgacc ggacccggag gcgtcggcaa gacgcgcctc gccgtgctcg
     3841 tcgccgagac gctgctgtcc gagtaccgcg aggacatccg gttcgtcgac ctgtccatgg
     3901 ttcgggatcc ggagcaggtg ctcgagcgca tcggcgcatt ggttggcggc ggcggtcgtc
     3961 cggaggaagc aatcggcgag cgacggatgc tgctcgtgct ggacaacttc gagcaggtgc
     4021 tcgacgcctc tgcttcgatt tcacacctca tcggccgctg tcccggtctc cgggtcatca
     4081 tcaccagccg ggcaccactc cggatcgccg ccgagcagca gctggatgtc ccgccgctcg
     4141 actcagctga ctcggcggag ctgttcatcg atcgggcagc ggccgccgtg gccgccgagg
     4201 ccctcccgac tgaggccgtg cgggaggtcg tcgcgcgcct cgatggcctg ccgctcgccc
     4261 tggagctcgc ggcggcacgg gtcaaggtcc tgacgatctc agcgatccgt gacttcctcg
     4321 ccgaccgcat gagcttcctg accgcaggcc agcgagacgc gcccgatcga catcggacac
     4381 tgcgcgacac cattcgctgg tcctatgagc tcctcgcccc cgaggcgcgt gcggtgttcc
     4441 gcaagctttc ggtgcttgct ccgggctttg acgtcgaggc cgccttcgcc gtcggcgaag
     4501 ccggactgga cgaagtcgcc gagctcgtcg accacagcct cctgcgtcgc gtcgaggatc
     4561 ggtacgccat gctcgaaacc attcgggcgt tcgcggaggg agcggcgagc gggcccgaag
     4621 ccaccgccgc tcacgaacgg caccttcgcc atttcacggc actgccgctc gcggcgcgcg
     4681 atcgcgttgc ccagctggcg ggctccctcc aacagggggc cgacgagtgg ctggacgtct
     4741 gtgcgagcaa ccaggagaac ctgcggctcg cgttcgagtg ggccagggcc actggcgacg
     4801 cccaggcaat ggtgagcctg ctccgcggcg tcggcgccta ctgggcgtac ctgggcacga
     4861 cggaggaggc tctgcgctgg gccgagatga cgctcgagcg gtccgaaggg ctgccacccg
     4921 ccgacctcga gcgtgtgcgg gtcggggctg ccgaattcgc ccgattcagc gaccagctgc
     4981 cattggcgct gtcactgctg cagtccgtgc tcgacggggc gacggagcgg gccgacctcg
     5041 tccgcattgc caacaccctg gacgacctcg ccaacacgta cgcctcgatg gatcgtgaaa
     5101 cggaggcact ggcgttgctc gggcgcgctc acgagacgca ccgccaggag cccaactgtg
     5161 acccgcggca cctcgggcat acgctcgagg tcgagatgtt cgtgctgctg cgactgggtc
     5221 gcgtcgacga agcagaggag acgtcgaagg cgtacgtcga ggtgaattcg acgagaccgg
     5281 gatggaccct gaggaacctc gagtccgagc tcgtcctcgc cgcgttggcc gcagccgcgg
     5341 ggcgacgtta cgatgcccgc gccggatatg gacgcgtcgt cgtcgcggcc tcacgcgggc
     5401 agttccgcgg catgatcgcg gacgcactgg atggcctcgc cggcatcgac cgcgacgagc
     5461 gcccaggcga cgccgccatg ctcctgggca tggccgatcg cgcccgtgcc gaatcgcgct
     5521 cgacggtatt cttcgcaccg caacgccggg cgttgatcga cgggctgcgc gagacgctcg
     5581 ggcctgacag gtacgaggcg gcgagaagtg caggggaggg cgtcccggtg atcgagatgg
     5641 cggcagccgc gctgccgagc aaacccgagc tggtcgcgga gcctgctgcc aggagcacct
     5701 gatcgggggt cgcgggaccc tgtgctacta tcccgcggcg gccggagggt cgcggctttg
     5761 ccgtgattca gccgggcgca ccaagcgacg tttgagaggg aagcaaccat cagccgagac
     5821 ctgcgcatca acgagatgat ccggatccca caggtccgag tgatcgacga tgagggcaac
     5881 cagctgggcg tcatgcccac gccgcgagcc ctggagatgg cgcaggagaa aggcctcgac
     5941 ctcgtcgagg tggcaccgat ggcggctccg ccggtcgttc gtttcctcga cttcgggcag
     6001 tacaagtacg agctcacgaa gcgggagaag gaggccaagc gtcgacagcg gattggcttc
     6061 aaggaagtcc ggctctcgcc gaagatcggc gttggcgact tcgacacgaa gctccatcgc
     6121 gcggtcgagt tcctcgagga cggcgatcga atcaaggtca cggtccgctt ccggggccgc
     6181 gagctcagcc atcccgaact ggggcgggtg ctgctcgaac ggttcagcaa gcaggtcgaa
     6241 gagcacgcga tcatcgaacg gcagccggtg ctcgagggca agtcgatgtt catcgtcctg
     6301 gcctcgaccc acaagccgaa ggtcgttgaa cacgaaccga agcaccgaga cgaacagcat
     6361 gacgcggaac cggctccggc cgagtccgca ccggtagcag caccggcggc cgaagcggct
     6421 gcggcgccca agccggcagc agcgcccaag ccggcggcaa cgccggtggc ggcggccaag
     6481 gcggcaccgg cagccaagcc ggagccggca gcggcgacgg aaccggcgac agcgaaggcg
     6541 ccgacgaatg gcgcagcggc accggccgcc gtatcgacaa cgggagactg aacgcgacga
     6601 tgccgaagat gaagagccac aaggcagccc agaagcgcat cggcgtgacc ggcagcggca
     6661 aggtcaagcg agtgaacgcg tggcggggcc accacctcga gatcaagtcg tcgcgccgca
     6721 cccgccgcta cgccggcatc tcgacgatga acaagaccca cgcaaagcag gtcaagcgcc
     6781 tgctgccgta cctctaggag cgggcagagc acatgtcacg agtcaagcgc ggcgtcaccg
     6841 cccacgcccg ccacaagcgc ctcctccagg acgcggaggg ccgcaagggc acccgttcgc
     6901 gcctggtcaa gccggcgcgc gaggcgctcc tccatgccct cgcctacgcg acccgcgacc
     6961 gcaagcagcg caagcgcgac ttccgcggcc tgtggatcgt ccgcatcaac gctgccgttc
     7021 gcagccaggg cgtgacgtac ggccggttca tccagggcct gaagaaggcg gacatccaga
     7081 tcgatcgcaa gatcctggcc gacatcgccg tgcgcgatgc cggcaccttt acccagatcg
     7141 tcgaggcggc gaagggcagc tgaccggcag ccccatccgc tcgacgagcc ctcgatcccg
     7201 agatcgaggg ctcgatcatt ttccgggccc gacaagcccg taccatcgac ccgcgacacc
     7261 cgagaaggag cccgtggacc tcgcccagct gaccagcgac ctcgatgcgc tcgccgcgga
     7321 gaccgcggcg gcgcttgccg ctgcgtctga tgtcgccgcg atcgaggcgc tcgagctcga
     7381 cgtcctcggc aagaaggggc gcctgaccgg cgtccttcgc gggatcggcg cgctgccggc
     7441 cgacgatcgc ccgaaggtcg gcgcggttgc caaccgcgtg cgctcgacga tcgaggcggc
     7501 catcgcggag cggcgcctgg tgctcggctc ggccgagctc gacgcccgcc tcctggccga
     7561 gacgatcgac gtcacgatgc cgggccggcc gatccgccgc ggcagcctcc acccgagcat
     7621 cgagtcgatg accgagatcg cccggatctt cggccagttc gggttccaag tctacgagac
     7681 gcccgagatc gaggacgacc aggccaactt ccaggacctc aacatcccgc ccgatcatcc
     7741 ggcgcgcgac ctgtgggaca cgctgtacgt ggacatcccg ggccggctcc tcagaactca
     7801 cacgtcacca ggccagatcc acgtgatgcg aaccgtcaag ccaccgatcc gcgcgctccc
     7861 gccggggcgg tgcttccggt acgaggcgat cgacgcctcc cacggctcgg agttctttca
     7921 ggtcgagggc ctgatgatcg acgagggcac gaccatgggc gacctgcgcg
//
LOCUS       DATBSW010000308         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_558564, whole genome shotgun sequence.
ACCESSION   DATBSW010000308 DATBSW010000000
VERSION     DATBSW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558564"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VIF63_08500"
     CDS             <1..1143
                     /locus_tag="VIF63_08500"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8239461.1"
                     /translation="GEITGVSLVFDYYAGAANKVFGQTIPVSKPGLDLVLREPIGVVG
                     LIVPWNFPLLMASWKLGPALAAGNTCILKPASLSPMTALRLGELALEAGFPPGVINVV
                     TGPGGTAGASIAAHPGIGKVAFTGETTTGQEIMRLASNNVKKISLELGGKSPNVVYAD
                     ADIERFASESPYSVFDNCGQDCCARSRILVERSVHEQVVELFAAATRKVVVGDPADDR
                     TEVGTLVSFKQRDRVRDYIEIGLAEGANLVVGGEAPDDPALADGAYLMPAVFDGVGND
                     MRIAKEEIFGPVVSIIPFETEAEAIQLANATPYGLSGSVWSRDIGKALRTAKGIQAGV
                     LSVNSNSSVHTEAPFGGYKMSGIGRELGMSAIELYTETKNIFIDLR"
     gene            complement(1217..1858)
                     /locus_tag="VIF63_08505"
     CDS             complement(1217..1858)
                     /locus_tag="VIF63_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8239462.1"
                     /translation="MTLDPRRAAGRGPDGRSTDLPFGGGPPEWLSASLNHCTRCGGRL
                     VLGPVPGDPRPRLTCADCGHIAYVNPKLVVSTLPITEAGEIVLLRRGFEPGRGLWAQP
                     GGFLEVDETATEGAVRETEEEIGLIVEPTEIVGLYARLEAAVIVLAYEARIVEGEMRT
                     SPEALDVRAFAPEAIPWPEIAFRTTWWMLVDWLARRRPDIDPPDERWTLSREG"
     gene            complement(1855..2253)
                     /locus_tag="VIF63_08510"
     CDS             complement(1855..2253)
                     /locus_tag="VIF63_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239463.1"
                     /translation="MDDPRWEPGMPVLDRQGIGADRAPDRIPETKPTPLQRYYINLSA
                     IALVAGAIGITALEAGAELSSPIVKFCALIATPLFVLTTADAALRFWRSAWAWMPIDR
                     GRGAFRILWACAALVGIGVAIGAASLILTA"
     gene            complement(2277..2780)
                     /gene="purE"
                     /locus_tag="VIF63_08515"
     CDS             complement(2277..2780)
                     /gene="purE"
                     /locus_tag="VIF63_08515"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY8239464.1"
                     /translation="MSDASRAPVVGVVGGSRSDFPVLEQAVAMLTALEVSSELRVVSA
                     HRSPDHLYRYAESAADRGIQVIIAGAGGAAHLPGMLAAKTRLPVIGVPMPTKHLDGMD
                     SLLSIVQMPRGIPVATVAIGNAENAALLAVEILALGDPALAARLDAYRADLTRMVIDD
                     PSNAEPL"
     gene            complement(2777..>4362)
                     /locus_tag="VIF63_08520"
     CDS             complement(2777..>4362)
                     /locus_tag="VIF63_08520"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY8239465.1"
                     /translation="IGSTSVPGLPGKGIVDLSIETEPDDIPGVVAMLYELGFGPQPGP
                     DPWPPTRPMPVGSLTLNGTSYRIHLHVQPRGGDAPRDIAFRDALRNDSELRRQYEGLK
                     RGITDAGPVDGLRYTHSKTAWILQVYRRLGFAPPPIAPPSTIGILGGGQLGRMLALAG
                     RELGYRVVVLDPDPAAPAGAIADRLEIGAYDDVAAAQRLAAESAVITYELEHIGAAVV
                     AALDDRRRPIRPGPYPLKLTADRLAERRFIEANEGTVATWREVGSPSDVLEAVAELGF
                     PVRVKAVRGGYDGRSQVRIDSMDGIEALDARIGWPALLERELAFEAELSVVVVRNVDG
                     VSSTFPVARNRHDRGILVETVVPAGIQPRIAAEAETLAKNLATSMGLVGTLTVELFLM
                     PDGALVVNELAPRVHNSGHWSIEGAVTSQFEQHLRAICGLPLGSVEMRAPAVAMVNLL
                     GEGAPRPAHPTGIYQALEVPDAHLHLYDKHTVFERRKMGHVTALGATREEALARVHEA
                     AAHIGWAPAAHDPAVEPVG"
BASE COUNT          648 a         1501 c         1614 g          599 t
ORIGIN      
        1 ggcgagatca ccggcgtgag cctggtcttc gactactacg ccggggcggc gaacaaggtc
       61 ttcggccaga cgatccctgt ctcgaagccc ggcctcgacc tggtcctgcg cgagccgatc
      121 ggcgtggtcg ggttgatcgt gccctggaac ttcccgctgc tcatggcttc gtggaagctg
      181 ggtccggcat tggccgcagg caacacctgc atcctcaagc ccgcctcgct ctcaccgatg
      241 acggcgctgc gactcggcga gctggccctc gaggcggggt tcccgcccgg cgtcatcaac
      301 gtggtcaccg ggcccggcgg caccgccggc gcatcgatcg cggcccaccc cggcatcggc
      361 aaggtggcat tcacgggcga gaccacgacc ggccaggaga tcatgcggct ggcctcgaac
      421 aacgtgaaga agatcagcct cgagctcggt ggcaaaagcc ccaacgtggt gtacgccgac
      481 gccgacatcg agcgcttcgc gtccgaatcg ccgtacagcg tcttcgacaa ctgcggccag
      541 gactgctgcg cgcggtcgcg catcctcgtc gagcgatcgg tgcacgagca ggtcgtcgag
      601 ctcttcgcgg cggcgacgcg caaggtcgtc gtcggcgacc cggcagacga caggaccgag
      661 gtcgggacgc tcgtcagctt caagcagcgc gaccgggtca gggactacat cgagatcggc
      721 ctcgccgagg gcgcgaacct cgtcgtgggc ggcgaggcgc cggatgatcc ggccctggcc
      781 gatggtgcgt atctcatgcc ggcggtcttc gacggtgtcg gcaacgacat gcggatcgcg
      841 aaggaagaga tcttcggccc cgtcgtgtcg atcatcccgt tcgagaccga ggccgaggcg
      901 atccagctcg cgaacgcgac gccatacggc ctgtcagggt ccgtctggag ccgggacatc
      961 gggaaggcgc tgcgaaccgc gaagggcatc caggcgggcg tgctgtcggt caactcgaac
     1021 agctcggtcc acaccgaggc gccgttcggc ggctacaaga tgtcgggcat cgggcgggag
     1081 ctgggcatgt cggcgatcga gctgtacacc gagacgaaga acatcttcat cgacctgcga
     1141 tgacggggcc ggcccagcgg ccgaccagct ccagcggccc gacggggcgc agagatcccg
     1201 ggtcgccgag tagctcctag ccctcgcgcg acagcgtcca gcgctcgtca ggcggatcga
     1261 tgtcgggccg gcggcgggcg agccagtcga ccagcatcca ccacgtcgtc cggaacgcga
     1321 tctcgggcca cgggatcgcc tcgggcgcga acgcgcgcac gtccagcgcc tcggggctcg
     1381 tgcgcatctc gccctcgacg atgcgcgcct cgtaggcgag cacgatcacg gccgcctcga
     1441 gtcgcgcgta caggccgacg atctcggtcg gctcgacgat cagcccgatc tcctcctcgg
     1501 tctcgcggac ggcgccctcg gtggcggtct cgtcgacctc caggaacccg ccgggctgcg
     1561 cccacagccc gcggccgggt tcgaaacctc ggcgcagcag gacgatctcg ccggcctcgg
     1621 tgatcggcag cgtcgagacg acgagcttcg ggttgacgta ggcgatgtgg ccgcagtcgg
     1681 cgcaggtgag ccgcggccgc gggtcgcccg gaacgggacc gaggacgagc cgcccgccgc
     1741 agcgggtgca gtggttgagc gaggccgaca gccactcggg cgggccgccg ccgaacggaa
     1801 ggtcggtcga gcgtccgtcc ggcccacggc cggcggcccg gcgcgggtcg agggtcacgc
     1861 cgtcaggatg aggctcgcgg ccccgatggc aacgccgata ccgacgagcg cggcgcaggc
     1921 ccacaggatc cggaaggcgc cccggccgcg gtcgatcggc atccacgccc aggcgctgcg
     1981 ccagaagcgg agcgcggcat cggcggtcgt caggacgaag agcggcgtgg cgatcagcgc
     2041 gcagaacttg acgatcgggc tcgagagctc ggcgcccgcc tccaacgccg tgattccgat
     2101 cgcgccggcc acgagggcga tcgcggacag gttgatgtag tagcgctgga gcggcgtcgg
     2161 cttggtctcc gggatccgat ccggtgcccg atcggcgccg atcccctggc gatccaggac
     2221 gggcatgccc ggttcccatc gggggtcgtc catggcggcc ggctcgtcgg cggcggtcaa
     2281 agcggctcgg cgttcgacgg gtcgtcgatg accatgcggg tcaggtccgc ccggtaggcg
     2341 tcgaggcgcg cggcgagcgc agggtcgccg agggcgagga tctcgacggc gaggagcgcg
     2401 gcgttctcgg cattgccaat ggcgacggtg gcgaccggga ttccgcgcgg catctggacg
     2461 atggagagca gcgagtccat gccgtcgagg tgcttcgtcg gcatcgggac gccgatgacg
     2521 ggcagcctcg tcttcgccgc gagcatgccc ggcaggtggg ccgcgccgcc ggcgcccgcg
     2581 atgatcacct ggatcccgcg atccgcggcg ctctccgcgt atcgatagag gtggtccggg
     2641 ctgcgatgcg ccgagacgac gcgcagctcc gacgacacct cgagggcggt gagcatcgcg
     2701 accgcctgct cgagaaccgg gaagtccgag cggctgccac cgacgacgcc gacgacgggc
     2761 gcgcgactcg cgtcgctcaa ccgaccggct cgacggctgg atcgtgtgcc gcgggcgccc
     2821 agccgatgtg cgcggcggcc tcgtgcaccc gcgcgagcgc ctcctcgcgt gtcgcaccga
     2881 gcgcggtcac gtggcccatc ttccggcgct cgaacaccgt gtgcttgtcg tacaggtgga
     2941 ggtgcgcgtc ggggacctcg agcgcctggt agatgccggt cggatgggcg ggccgcggcg
     3001 cgccctcgcc gagcaggttg accatcgcca cggccggcgc acgcatctcg accgagccca
     3061 gcggcaaccc acagatcgcc cgaaggtgct gctcgaactg gctcgtcacc gcgccctcga
     3121 tcgaccagtg cccgctgttg tggacgcgcg gcgccagctc gttgaccacg agcgcgccat
     3181 cgggcatcag gaagagctcg accgtgagcg tgcccacgag gcccatcgac gtcgccaggt
     3241 tcttggcgag cgtctcggct tcggcggcga tccggggctg gatcccggcg gggacgacgg
     3301 tctcgacgag gatcccgcgg tcgtgccggt tgcgcgcgac cgggaacgtc gagctgacgc
     3361 catcgacgtt gcgcacgacc acgaccgaca gctcggcctc gaacgcgagt tcgcgctcga
     3421 gcagggcggg ccaaccgatc cgcgcgtcca gcgcctcgat gccgtccatg ctgtcgatgc
     3481 gaacctggct ccggccgtcg tagccgccgc gaaccgcctt gacccggacc gggaagccga
     3541 gctcggcgac ggcctcgagg acatcgctcg ggctgccgac ctcgcgccag gtcgcgaccg
     3601 tgccctcgtt cgcctcgatg aaccggcgct cggcgagacg gtcggcggtg agcttgagcg
     3661 ggtacggacc ggggcggatc gggcgtcgcc ggtcgtccag ggccgccacg acggcggccc
     3721 cgatgtgctc gagctcgtag gtgatgacgg cagactccgc agcgaggcgc tgcgccgcgg
     3781 cgacgtcgtc gtacgcgccg atctcgagcc ggtcggcgat ggcccctgcc ggcgcagccg
     3841 ggtcggggtc gagcacgacc acgcggtagc cgagctcgcg acccgcaagg gcgagcatcc
     3901 ggccgagctg gccgccgccg aggattccaa tcgtcgatgg cggcgcgatc ggcggtggtg
     3961 caaagccgag ccggcgatag acctgcagga tccaggcggt cttcgaatgc gtgtagcgca
     4021 ggccgtcgac cggcccggcg tcggtgatgc cgcgcttgag gccctcgtac tggcgacgga
     4081 gctcggagtc gttgcgaagc gcgtcgcgga aggcgatgtc gcgcggggca tcgccaccgc
     4141 gcggctggac gtggaggtgg atccggtagc tcgtgccgtt gagcgtcagc gagccgacgg
     4201 gcatcggccg ggtcggcggc cacgggtcgg ggcctggctg cgggccgaaa ccaagctcgt
     4261 agagcatcgc gaccacgccc gggatgtcgt ccggctcggt ctcgatcgag aggtcgacga
     4321 tgcccttgcc gggcaggcct gggaccgagg tgctgccgat gt
//
LOCUS       DATBSW010000309         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_699789, whole genome shotgun sequence.
ACCESSION   DATBSW010000309 DATBSW010000000
VERSION     DATBSW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699789"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VIF63_08525"
     CDS             <1..246
                     /locus_tag="VIF63_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239466.1"
                     /translation="DVALAAARAMDSERWGRYLEPLPGRLRDDQLRDLRNAARLARAA
                     YGPKDSIRDALPEYATEPLLKAIDRLLKSIARYEANR"
     gene            complement(262..723)
                     /locus_tag="VIF63_08530"
     CDS             complement(262..723)
                     /locus_tag="VIF63_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY8239467.1"
                     /translation="MAVQAKTYVSWDDLDEMVGSLAEQVGTDYDVVLAITRGGLVPAG
                     MLAYRLGFRNILVAAVVYYDDAGQPAEQPTFFQFPSDPLLHDRRVLIIDEVWDTGTTI
                     AAVVGRVELAGGRPTTAVLHYKPARSKVELVPDYHVAATDDWVVYPFKHGN"
     gene            1131..>1567
                     /locus_tag="VIF63_08535"
     CDS             1131..>1567
                     /locus_tag="VIF63_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8239468.1"
                     /translation="MRPDKVDAYAAQGVMPNMQALAASGVKGVNGMQPGFPPNTGVGW
                     YTMMTGTWPSEHGSTNNTYHRTGESNFNNRTSFSALQNGAPTLQADTLAAAAERAGKK
                     VAQIDWVGGAAANIAGPTVDFTSFFTNRGVLVGAADPVEQAGSA"
BASE COUNT          247 a          557 c          522 g          241 t
ORIGIN      
        1 gatgtcgcgc tggccgcggc tcgcgcgatg gactcggagc gctggggccg ctacctcgag
       61 cccctcccgg gccgtctccg cgacgaccag ctgcgcgacc ttcgaaacgc ggcgcgcttg
      121 gcgcgagccg cctacggccc gaaggactcc attcgtgacg cgctgccgga gtacgccacg
      181 gagccgttgc tcaaggccat cgaccgactc ctgaagtcga ttgcccgcta cgaggcgaac
      241 cgctagcacg cggtcgccgg gtcagttccc gtgcttgaac gggtagacga cccagtcgtc
      301 cgtcgcggcg acgtggtagt cggggaccag ctcgaccttc gaccgcgcag gtttgtagtg
      361 cagcaccgcg gtcgtcgggc gaccgcccgc cagctcgacg cggccgacga cggccgcgat
      421 cgtcgtgccg gtgtcccaga cttcgtcgat gatcaggacg cgccgatcgt gcagcaatgg
      481 gtccgagggg aactggaaga acgttggttg ctcggccggt tgacccgcgt cgtcgtagta
      541 cacgaccgcc gcgacgagga tgttccggaa gccgagccgg tacgccagca tgcccgcggg
      601 cacgagcccg ccgcgagtga tcgccaggac gacgtcgtag tcggtgccga cctgctcggc
      661 gaggctgccg accatctcgt cgaggtcgtc ccaactgacg tacgtcttcg cttgcacggc
      721 cacgagcaca ggctaccgga cccgaggcac gccgggttca caactcggaa ttcgagcgct
      781 catgccccga tgacgttacg ggcctttgat gacccggtgc tggagtgttg atggattcgc
      841 aaggccttcg gcgcagtctg gtcgtgggcc gtcagggagg ttgaccggcc cgactcaccc
      901 atggcgtgcc ttcgcgccac gcccagcccc gcgctcccga tcaacgctcg cgcccatcgt
      961 tgtggaggtg ctccgtggct ccgtcaggac ttgtacgcgt tcgatcccgc ctcgcggccg
     1021 tcggcgccgc gctcgtgctc ctcgcgacgt cgttggtcat acccgccagc gccgcgcccc
     1081 acgcatccgg tccacttgcg acgagagccc tcttctatgc cgccgacggc atgcggcccg
     1141 acaaggtcga tgcctacgcc gcccagggcg tcatgcccaa catgcaggcg ctggccgcgt
     1201 cgggcgtgaa gggcgtgaac ggcatgcagc cgggcttccc gccgaacacc ggcgtgggct
     1261 ggtacacgat gatgaccggc acctggccga gcgagcacgg ctcgacgaat aacacgtacc
     1321 accgaaccgg tgagagcaac ttcaacaatc ggacgagctt cagcgcgctg cagaacggcg
     1381 cgcccacgct ccaggcagat accctcgccg ccgccgcgga gcgcgccggc aagaaggtcg
     1441 cccagatcga ctgggtcggc ggcgccgcag cgaatatcgc gggcccgacg gtcgacttca
     1501 cctcgttctt caccaaccga ggcgtgctcg tcggtgcggc cgatccggtc gaacaggcgg
     1561 gctccgc
//
LOCUS       DATBSW010000310         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1193728, whole genome shotgun sequence.
ACCESSION   DATBSW010000310 DATBSW010000000
VERSION     DATBSW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193728"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VIF63_08540"
     CDS             <1..375
                     /locus_tag="VIF63_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8239469.1"
                     /translation="FPYMYRAVDTGLAAIDVRSLTEASQSLGAGWTRILLRVIFPNLR
                     TAILSGAFLTLAIVVGEFTIATYLARNAAFGPYISLLGRTQPYTPAVISLVSFALTWL
                     AMGLIAYIGRGSRTRISVAGAK"
     gene            419..1528
                     /locus_tag="VIF63_08545"
     CDS             419..1528
                     /locus_tag="VIF63_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239470.1"
                     /translation="MSYLTLADVQKRFGEFSAVQNFNLAAEKGEFVSFLGPSGCGKTT
                     TLRMIAGFEQPTSGTITIDGRDVTRVPPNRRNVGMVFQSYALFPNMTVADNVGFGLKV
                     RKRPPDEIRKRVAELLETVNLPDKGGRYPYQLSGGQQQRVALARALAFEPQVLLLDEP
                     LSALDAKIRVALRHEIRDIQRRLGITTVYVTHDQEEALSLSDRVVVMSEGRMEQVGTP
                     FEIYNFPSTAFVASFVGTLNVLAGVVTDAARGELTISGQPVRIASSFEGNRGREVSVA
                     LRPEMVSLGGDAPNGANRFTGKVTDVSFLGSIVRIRVALGGDGPTVVLDEFNEPTLKL
                     PTLGETVAISFPADGPLVLDAKAPVESVEALIAEK"
     gene            1550..>2273
                     /locus_tag="VIF63_08550"
     CDS             1550..>2273
                     /locus_tag="VIF63_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8239471.1"
                     /translation="MAAGAINALDPLDGIDLLIFDKDGTLIEFHLMWGAWVEELAARL
                     EAASGLALREGLYPLLGVDRATGLVHAHGLLAATPMSRIREVVQAHLEESGAGPSAAA
                     AAVASAWHAPDPVALAQPVTDLRALLGGLRRRVATFAVATSDDRRPTERTLEALGVAA
                     EFAQLVCADDGVRNKPAPDPVLHLCGSLGIPPGRPAVVGDSPADLLMAREAGVARGIG
                     VLTGVGDRASLEPLADVVLGSIA"
BASE COUNT          321 a          826 c          774 g          352 t
ORIGIN      
        1 ttcccctaca tgtacagggc cgtggacacg ggcctcgcgg cgatcgacgt cagatcgctg
       61 acggaggcgt cgcagagcct tggcgcgggc tggacccgga tcctccttcg ggtcatcttc
      121 ccgaacctcc ggacggcaat cctgtccggc gccttcctga cgctggcgat tgtcgtcggc
      181 gagttcacca tcgcgacgta cctcgcccgc aatgcggcat tcgggccata catctcgctg
      241 ctcggccgga cccagccgta cacgccggcc gtcatctcgc tcgtcagctt cgccctgacc
      301 tggctcgcca tgggcctcat cgcgtacatc gggcggggct cgcggacgcg gatctcggtc
      361 gcgggcgcaa agtgaccagc cggaccgcca accccaatcg ccggaggacg atccctcgat
      421 gagctacctc accctcgccg acgtccagaa gcggttcggc gagttctccg cggtccagaa
      481 cttcaatctc gccgcggaga agggcgagtt cgtgtcgttc ctcgggccgt ccgggtgcgg
      541 caagacgacc acgctccgga tgattgcggg cttcgagcag cccacctccg gcacgatcac
      601 gatcgatggg cgcgacgtca cccgcgttcc gccgaaccgc cgcaacgtcg gcatggtctt
      661 ccagtcgtac gccctcttcc cgaacatgac cgtcgccgac aacgtcgggt tcggcctcaa
      721 ggtccggaag cggcccccgg acgagatccg gaagcgtgtc gcagagctgc tcgagaccgt
      781 caacctgccc gacaagggcg ggcgctaccc gtaccagctc tccggcggcc agcagcagcg
      841 cgtcgccctc gcgcgggccc tggcgttcga gccgcaggtc ctgctccttg atgagccgct
      901 ctcggccctc gacgccaaga tccgcgtcgc gctgcggcac gagatccggg acatccagcg
      961 gcgcctgggg atcaccacgg tctacgtgac ccacgaccag gaggaggcgc tctccctgtc
     1021 cgatcgtgtc gtcgtcatga gcgaaggccg gatggagcag gtcgggacgc cgttcgagat
     1081 ctacaacttc ccatcgacgg cgttcgtggc ttcgttcgtc ggcacgctca acgtgctggc
     1141 cggtgtggtc accgatgccg cgcggggcga gctgacgatt tccggccagc ccgtgcggat
     1201 tgccagctcc ttcgagggca atcggggacg cgaggtcagc gtcgccctcc ggccggagat
     1261 ggtctcgctc ggcggcgacg cgcccaacgg cgccaaccgc ttcaccggca aggtcacgga
     1321 cgtcagcttc ctcggctcga tcgttcggat ccgcgtcgcg ctgggcggcg acgggccgac
     1381 cgtggtcctc gacgagttca acgagcccac cctcaagctg ccgaccctgg gcgagaccgt
     1441 cgcgatcagc ttcccggccg acggcccgct cgtgctcgac gcgaaggcgc cggtcgaatc
     1501 ggtcgaggcc ctgatcgccg agaagtaggt ccggtcggaa ccgcgcgtca tggcggcagg
     1561 cgcgatcaat gcgctcgatc cgctcgatgg catcgacctg ctgatcttcg acaaggacgg
     1621 cacgctgatc gagttccacc tgatgtgggg cgcgtgggtc gaggagctgg cggcccggct
     1681 cgaagccgcc tccgggctgg ccctgcgcga aggcctgtac ccgctgctcg gcgtcgatcg
     1741 cgcgaccggc ctcgtccatg cccacgggct gctcgccgcg acgccgatgt cgcggatccg
     1801 ggaggtcgtc caggcgcacc tcgaggaatc cggcgccgga ccgtcggcag cagcggccgc
     1861 ggtggcgagc gcctggcacg ctcctgatcc cgtcgcgctc gcacagccgg tgaccgacct
     1921 gcgcgcgctc ctcggcgggc tgcggcgccg agtggccacg ttcgcggtag ccaccagcga
     1981 cgatcgccga ccgacggagc ggacgctcga ggcgctcggc gtcgcggccg agttcgcgca
     2041 gctcgtctgt gccgacgacg gcgtccggaa caagcccgcg ccggatccgg tcctccacct
     2101 gtgcggatcc ctgggcattc cgcccggccg acccgcggtg gtcggggact cgccggcgga
     2161 cctgctgatg gcgcgcgagg ccggcgtggc gcgcgggatc ggcgtgctca ccggcgtcgg
     2221 cgaccgagcg agcctcgagc cgctggccga tgtcgtgctc ggctcgatcg cgg
//
LOCUS       DATBSW010000311         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1016883, whole genome shotgun sequence.
ACCESSION   DATBSW010000311 DATBSW010000000
VERSION     DATBSW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016883"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VIF63_08555"
     CDS             complement(<1..770)
                     /locus_tag="VIF63_08555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8239472.1"
                     /translation="MVEHARAVIIGGGVGGTSIAYHLTELGWRDVVLVDRAELTSGST
                     FHSAGLVGQLRSSTTLTRMMMYGAELYRRLTAETGVDVSWHEVGSLRLASTKERFEEL
                     RRQAGWAKTFGLPLELITPAEAQAKFPLMTLDGVLGAVWLPTDGWLDPSGLAQALAAG
                     ARSRGATIRQHTRVVGIDVRDGQVRGVEVEGRGGERTTIEAEVVVNAGGMFAPEIGRL
                     AGVTVPIIPMAHQYLFTNAIEGAVPSLPTMRDPDRLCY"
     gene            845..>1750
                     /locus_tag="VIF63_08560"
     CDS             845..>1750
                     /locus_tag="VIF63_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY8239473.1"
                     /translation="MTVTGSGWTKRHWLALGAAALVGAAIRGFYWADPGFSDDLGEFA
                     RLTAAMVDHGLGRAYDAKLSFGPVVTYFWWLASLVQPAFAGAVDSSDLGVRLLLKLPP
                     ILADFGIAALVAYALRARPTWAIVGAAIILLHPAVWFVSSWWGQYESVFTFFALLAFV
                     LVLRDRPLLAAIAITAAILTKPQALALALPFAAWYLATYDRRTVIRAGMTAVVVAIAL
                     WLPFLQWDGPSKYLGWLAYYHDERFDVISMRAWNVWWIVQRNILHVSWVPDEDSLVGP
                     LTYRMAGYLITVALAALVAYRVWRRP"
BASE COUNT          229 a          639 c          593 g          289 t
ORIGIN      
        1 aagtagcaga ggcggtccgg gtcgcgcatc gtcggcaggc tcgggacagc gccctcgatc
       61 gcgttcgtga agaggtactg gtgggccatc ggtatgatcg ggacggtgac gccggcgagg
      121 cgcccgatct cgggcgcgaa catcccaccc gcgttgacga ccacctcggc ctcgatggtc
      181 gtgcgctccc ctccccgacc ttcgacctcg acgccgcgga cctggccgtc gcgcacgtcg
      241 atgccgacga cgcgggtgtg ctggcggatc gtggcgcccc gggagcgcgc acccgccgcg
      301 agcgcttggg ccagcccgga cgggtcgagc cagccgtcgg tcggcagcca gaccgcgccg
      361 aggacgccgt cgagcgtcat gagcgggaac ttcgcctgcg cctctgcggg cgtgatcagc
      421 tcgagcggca ggccgaacgt cttcgcccag ccggcctggc gccggagctc ctcgaagcgc
      481 tccttggtcg acgccagccg aagcgagccg acctcgtgcc acgacacgtc caccccggtc
      541 tcggcggtga gccgtcggta cagctcggca ccgtacatca tcatccgggt cagggtggtc
      601 gacgaccgca gctgcccgac gagccccgcc gagtggaagg tcgagccgga cgtgagctcc
      661 gcccgatcga ccaggacgac gtcgcgccag ccgagctcgg tcaggtggta ggcgatcgac
      721 gtcccgccga cgccgccacc gatgatcacc gcccgcgcgt gctcgaccaa cccgccctcc
      781 ctcgaattcc cgcaaccgtc gttgcggagc gtaccggctc gtgcgcccgc tacgctgcgg
      841 cccgatgacg gtcaccggct ccggctggac aaagcgccac tggcttgcgc tcggcgcggc
      901 tgcgctcgtc ggcgctgcga tccgcggctt ctactgggct gacccggggt tctcggacga
      961 tctcggcgaa ttcgcacgcc tgaccgcggc gatggtcgat cacgggctcg ggcgcgcgta
     1021 cgacgcgaag ctgtcgttcg gcccggtcgt gacctacttc tggtggctcg cgagcctcgt
     1081 tcagccggcc ttcgccggtg cagtcgactc gagcgacctc ggcgtgcgcc tgctgctcaa
     1141 gctgccgccg atcctggccg acttcgggat cgcagcgctc gtggcgtacg cgctccgagc
     1201 gcgaccgacg tgggcgatcg tgggcgccgc gatcatcctc ctgcacccgg cggtctggtt
     1261 cgtgagctcc tggtggggcc agtacgaatc ggtcttcacg ttcttcgccc tgctcgcgtt
     1321 cgtcctcgtg ctgcgcgatc gaccgctgct cgcggcgatc gcgatcaccg cggcgatcct
     1381 gacgaagccg caggccctgg cgctcgcact cccgttcgcc gcctggtatc tcgcgaccta
     1441 cgatcgccgc acggtcatcc gagccgggat gaccgcagtg gtcgtcgcca tcgcgttgtg
     1501 gctgcccttc cttcagtggg acggtccgag caagtacctc gggtggctcg cctactacca
     1561 tgacgagcgg ttcgacgtca tctcgatgcg ggcctggaac gtgtggtgga tcgtccagcg
     1621 gaacatcctc cacgtctcgt gggtgcccga cgaagactcg ctcgtcggtc cactgacgta
     1681 ccgcatggcc ggctacctga tcacggtcgc cctcgcggcg ctcgtcgcgt accgggtctg
     1741 gcgccggccg
//
LOCUS       DATBSW010000312         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_982700, whole genome shotgun sequence.
ACCESSION   DATBSW010000312 DATBSW010000000
VERSION     DATBSW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982700"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VIF63_08565"
     CDS             complement(<1..448)
                     /locus_tag="VIF63_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8239474.1"
                     /translation="MAEAPTAPTLIIPTPDGRSLDVWLAGPADGRPVLFHSGTPGAGM
                     PFDQHVAAMADRGLRYVSVTRPGYGGSSRREGRSVADVVDDVRTVLDHLGIDRTFVLG
                     WSGGGPHAIATAALLPERVLGTAVMAGVAPFDAQGLDWLAGMGEENV"
     gene            complement(720..2075)
                     /gene="hflX"
                     /locus_tag="VIF63_08570"
     CDS             complement(720..2075)
                     /gene="hflX"
                     /locus_tag="VIF63_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8239475.1"
                     /translation="MPSTKTNAKTNAKTNAKTTATKLIDLEVPVERAFLLAVDTGADD
                     GWNAEDSLAELANLALTAGAEVVGAEWQNRRHIDPHWYVGKGKAEDLVAAKSETGFDL
                     LVADDELSPTQQKTLEELVKVKVIDRSRLILDIFALHARTHEGRLQVELAQLEYQLPR
                     LTKLWTHLSRTQGGIGSRGPGESQLETDRRIIRDRIKKMKERVDDVRQARATAARGRD
                     RKLMPTVGIVGYTNSGKSTLLNKLVGSVVTLAEDKLFATLDPTSRQVKLGDGQTAILT
                     DTVGFIHKLPHQLVDAFRATLEEVNRADVLIEVVDAADAHAAEHRATVQSVLDELGAG
                     DKPRLVAWNKADLVAAAATNGAGAGPAVAGTVLVSALTGFGLDTLRVELAALLAELWE
                     DVDVRLPYTAGELLARVRERGTIELDYTGTDVHVHGRVAPALAAELRGRAVDGSTELG
                     S"
     gene            2121..2831
                     /locus_tag="VIF63_08575"
     CDS             2121..2831
                     /locus_tag="VIF63_08575"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEY8239476.1"
                     /translation="MTTERLLGIVGGVGPESTADYYRRLIDRWKARGPDGSYPPILLN
                     SLNSRPALDAMLAGDLEPTVRLFDASVARLAAGGAGLAIVASVMMHMTFDRVVATAPI
                     PMLSILDALVVEAGRRGIRRPGVLGTRPTTEGAFFAQPFEAAGIELIRPVEADRAFVH
                     EAYFGQLVRGDFRDETRAGLVAVIERMRERDGIDAVILGGTELPLILREPTYAGVPAL
                     DSTGIHVEAALDWLLDAG"
     gene            complement(3052..>4110)
                     /locus_tag="VIF63_08580"
     CDS             complement(3052..>4110)
                     /locus_tag="VIF63_08580"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8239477.1"
                     /translation="SAALAIALRSKPPRGKPPAIDPTTTALRERRLVRSWAMRGTLHL
                     FAADDVPTMSAALGGKDNWRRPAWLRWFGVTEPEMDALIDAVGDILDDGRPRTRAELA
                     EEITTRHGEKQGNLLRGSWGSALKVASDRHYLVQSSEDESGVRFVKASRWIAAWRDED
                     PIAALATLVQRYLAAYGPATLKEILRWWGVSVVKVMQPIVESLGDALTEVDVDGTRAY
                     VRTDDLAAIEATRPTHGEVHLVGGFDPFIVGAGLREHLIQAAHLKRVSRSAGWISPVV
                     LVDGRAAGVWDSARSSKGLTITIDPFAPFDRTRKAAVEAAAERVAKALGAPATVTYGR
                     VFTAEAKGPKLTINPGDA"
BASE COUNT          575 a         1439 c         1448 g          648 t
ORIGIN      
        1 gaacgttctc ctcgcccatg ccggcaagcc agtcgaggcc ctgggcatcg aatggcgcca
       61 caccggccat caccgcggtc cccagcaccc gctcgggcag cagcgccgcg gtcgcgatcg
      121 cgtgcgggcc gccgcccgac cagccgagga cgaacgtccg gtcgatgccc aggtggtcaa
      181 gcacggtgcg aacgtcgtcg acgacatcgg cgacggaccg gccctcgcgc cgcgacgagc
      241 cgccgtagcc cggcctggtc acgctgacgt accggaggcc gcggtcggcc atcgccgcga
      301 cgtgctgatc gaagggcatg ccggcgcccg gggtgccgga gtggaagagg acgggccggc
      361 catccgcggg tcccgcgagc caaacgtcga gcgaccggcc atcgggggtc gggatgatca
      421 gggtcggggc ggtcggggct tcggccacgg gccaatgatg gcggatcggg agggccgctg
      481 agcgacggcg gggccgctcc gggccgctcg gggatcagcg gggatcaccg ggcagaatca
      541 aacgcccaga ggaacctcgc tcccttttcg atcccaacct cacctgatgc aagcacctgg
      601 ttcggagtcc ggagcaagcc ccggacgaga tcgctaggtt ttcgtggccc ctctgggcaa
      661 gagaaatata ccggggcggg aaaccctccc gatacccact tatccacgag tttcgcggtt
      721 caggacccca attccgtgct tccgtccaca gcccgtccac gaagctcggc cgcaagggcc
      781 ggcgcgacgc gtccgtggac gtgcacgtcg gtgcccgtgt agtccagttc gatcgtgccc
      841 cgctcgcgca cgcgcgcgag cagttccccc gccgtgtacg gcaatcggac gtcgacgtcc
      901 tcccacagct ccgcgagcag cgccgccagc tcgacgcgca gcgtgtccag cccgaacccg
      961 gtcagcgccg agaccaacac cgtgcccgca acggccggcc ccgcccctgc tccgttcgtc
     1021 gcggccgccg caaccaggtc cgccttgttc caggcaacga gccggggctt gtccccggcg
     1081 cccagctcat cgagcacgga ctggacggtc gcgcggtgct cggccgcgtg ggcatcggcg
     1141 gcatccacga cctcgatcag gacgtcggcg cgattcactt cttccagggt cgcccggaac
     1201 gcatcgacga gctggtgcgg gagcttgtgg atgaagccca ccgtgtcggt gaggatcgcc
     1261 gtttgcccgt cgccgagctt gacctggcgc gaggtcgggt cgagcgtcgc gaagagcttg
     1321 tcctcggcca gcgtcacgac gctgccgacg agcttgttga gcagggtcga cttgccggag
     1381 ttcgtgtacc cgacgatgcc gaccgttggc atgagcttgc gatcgcggcc gcgcgctgcc
     1441 gtggcccgcg cctggcgcac gtcgtcgacg cgttccttca tcttcttgat ccggtcgcgg
     1501 atgatccggc ggtcggtctc gagctggctc tcgcccgggc cccgcgagcc gatgccgccc
     1561 tgggtgcgcg agaggtgcgt ccacagcttg gtgaggcgcg ggagctggta ctccagctgc
     1621 gcgagctcga cctggagccg gccctcgtgg gtgcgcgcgt gcagggcgaa gatgtcgagg
     1681 atcagccggc tgcggtcgat gaccttgacc ttgaccagct cctcgagggt cttctgctgg
     1741 gtcggcgaga gctcgtcgtc cgcgacgagg aggtcgaagc cggtctccga cttggccgcg
     1801 acgagatcct cggccttgcc cttgccgaca taccagtgcg ggtcgatgtg gcggcggttc
     1861 tgccactcgg ccccgaccac ctccgcgccg gcggtcagcg cgaggttggc cagctcggcg
     1921 agggaatcct cggcgttcca gccatcgtca gcgccggtgt cgaccgcgag caggaaggcg
     1981 cgctcgaccg ggacctcaag gtcgatcaat ttcgtggcgg tcgtcttcgc gttcgtcttc
     2041 gcgttcgtct ttgcgttcgt cttcgtgctc ggcatccggc gaaggatacc ggcccgcctc
     2101 cgagtgccag aattcgggac atgacgaccg aacgattgct cgggatcgtg ggcggggtcg
     2161 gccccgagtc gacagccgac tactaccgcc gcttgatcga tcgctggaag gcgcgcggcc
     2221 cggacgggtc gtacccgccg atcctgctga acagcctgaa ttcacggccg gcgctcgacg
     2281 cgatgctcgc cggcgacctc gagccgacgg tccggctgtt cgacgcttcc gtcgcccggc
     2341 tcgcggccgg tggcgccggc ctcgcgatcg ttgcctccgt gatgatgcac atgaccttcg
     2401 accgggtcgt ggcgaccgcg ccgatcccga tgctgagcat cctggacgcg ctcgtggtcg
     2461 aggccgggcg ccgcggcatc cgccggcccg gggtcctcgg aacacgaccg acgaccgagg
     2521 gcgcgttctt cgcccagccg ttcgaggcgg ccggcatcga gctgattcgg cccgtcgagg
     2581 cggaccgcgc cttcgtgcat gaggcgtact tcggccagct ggtccggggc gatttccgcg
     2641 acgagacgcg agccggcctg gtcgcggtca tcgagcgaat gcgcgagcgc gacggcatcg
     2701 acgccgtgat cctcggcggc acggagctac ccctcatcct gcgcgagccg acgtatgccg
     2761 gcgtgcccgc attggactcg accggcatcc acgtcgaggc cgccctcgac tggctcctgg
     2821 acgccggctg aattcttcgc gacccgggct gaagattctt cgcgacctgc gtgatgcgta
     2881 tgggctaatc gtcgcagtct cggccggcgc cgggacggca accgagggcc ggcggcggac
     2941 ggggcctcga tgacgcggcg ctcctgcgat cgtcgtcgag gttgggtgca ggacccggtc
     3001 gcacggagtg gtgattaacc cataggcatc gccgaccaca aaggaaatgt ttcaggcgtc
     3061 cccgggattt atcgtgagct tcgggccttt cgcctcggcg gtgaagacgc ggccgtaggt
     3121 gacggttgcc ggcgcgccga gcgctttggc gacgcgttcg gccgcggcct cgactgcggc
     3181 ttttcgcgtt cgatcgaatg gcgcgaacgg gtcgatcgtg atcgtcaggc ctttgctcga
     3241 gcgcgcgctg tcccacacgc ccgccgcgcg gccgtcgacg agcaccaccg gcgagatcca
     3301 gccggcgctc ctggacacgc gcttcaagtg cgccgcctgg atcaggtgct cacggaggcc
     3361 cgcgcccacg atgaacgggt cgaagccgcc gacgaggtgg acctcgccgt gcgtcggccg
     3421 cgtggcctcg atcgcggcga gatcgtcggt tcgcacgtac gcgcgggtgc cgtcgacgtc
     3481 gacctcggtc agggcgtcgc cgagcgactc gacgatcggc tgcatgacct tgacgaccga
     3541 cacgccccac cagcgcagga tctccttgag cgtcgccggg ccgtacgcgg cgaggtagcg
     3601 ctggacgagg gtcgcgaggg ccgcgatcgg gtcctcgtcg cgccacgcgg cgatccagcg
     3661 cgacgccttc acgaaccgca cgcccgattc gtcctccgac gactggacga ggtagtgccg
     3721 gtcggacgcg accttgagcg cgctgcccca gctgccccgc aggaggttgc cctgcttctc
     3781 gccgtgcctg gtcgtgatct cttcggccag ctcggcacgg gtgcgcggcc ggccgtcgtc
     3841 gaggatgtcg ccgacggcat cgatgagcgc atccatctcc ggctcggtca cgccgaacca
     3901 gcgcagccag gccggtcggc gccagttgtc cttgccgccg agcgcagcgg acatcgtcgg
     3961 gacgtcgtcc gcagcgaaga ggtggagcgt gccgcgcatc gcccacgatc ggacgaggcg
     4021 gcgctcccgc agcgcggtcg tcgtcgggtc gattgccggt ggcttgccgc gcggcggctt
     4081 cgagcgcagc gcgatcgcga gcgcggccga
//
LOCUS       DATBSW010000313         6284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_488267, whole genome shotgun sequence.
ACCESSION   DATBSW010000313 DATBSW010000000
VERSION     DATBSW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488267"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..727)
                     /locus_tag="VIF63_08585"
     CDS             complement(95..727)
                     /locus_tag="VIF63_08585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8239478.1"
                     /translation="MATSESTRARRFALPALDDERTGESIEAFLEGPDVRRGRPATLA
                     ESDPTVDSTTTTPAHRPTAPARPSTDLRSLPGRLEWNAALERESVRAARYQRPASVAI
                     IELKAERDGQSVDPWLGALAGPIARSLRQDSRATDLVARVASTRFQMLLPETPETGAE
                     QLAARVAATCREAIQRTGAPVTVRVSVAGTGLDNSLHEALAHALRSIEAA"
     gene            1261..1671
                     /gene="erpA"
                     /locus_tag="VIF63_08590"
     CDS             1261..1671
                     /gene="erpA"
                     /locus_tag="VIF63_08590"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY8239479.1"
                     /translation="MIEQHPVVEPQPIVDTTVLSLSDAAATKLRELTKEETNPNIGLR
                     VYVYSGGCSGFRYGMMLEDQPTPEDNILEVNGTKVYVDTNSIPLLKGSEIDYVDTLMG
                     AGFTVNNPNAVTACGCGSSFRTADDSGTARSCAH"
     gene            1893..3686
                     /locus_tag="VIF63_08595"
     CDS             1893..3686
                     /locus_tag="VIF63_08595"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8239480.1"
                     /translation="MTIASPPTALEAALRQVDEIADKTFAGWHVPGLSYGVVLGDRLI
                     HARGLGTLRVGEDATPTPSSVFRIASMTKSFTAATVLSLRDDKLLALDDPIARYVPEL
                     ADLRYPTTDSPPITIRHLLTMTAGFPTDDPWGDRQQALDLAAFSRLLSNGLSFAWAPG
                     TRFEYSNTGYGILGRLITNVVGREYKDVVRDRMLRPLGMNATGYEESEVPEERKAMGY
                     VWRDGTYLPEGMDPYGALAAMGGIFTSVEDLATWVSGFIDAVPPRDGPDISHPLPRAT
                     RREMQQPMVPTGLRTTHTSADAVTEVEAGAYGYGLFTVDDVRYGQTVGHSGGYPGFGS
                     NMRWHQASGLGVIALTNHRYGAATLLTRELLNELIRADAVPVRRVRPNAATEAAREAV
                     EGLIGAWDDDTAAALLAMNVDLDEPIGSRRAFVERLRARHGALRRDESEPVESLTSFH
                     AIWWLAGERGRVRVEILLSPELPPKVQTFGLTSVPEPPARLRHAAERLLGVMQPTDSG
                     PVAIDWPADLTVGTTVDVGAVVRAMRATEARFGPISLGPPIAGDGETKVTYRLISPRG
                     RVDLALELDSIADCLSAVSLVPAKQVPPDLD"
     gene            complement(3716..4339)
                     /locus_tag="VIF63_08600"
     CDS             complement(3716..4339)
                     /locus_tag="VIF63_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY8239481.1"
                     /translation="MRPEAAALYERLFGNKERDAAVADRIPPGQYRTEKFPVLHYGSV
                     PRTDLATWDFKVYGQVDSPFTLNWHQFKALPRKSVATDIHCVTRWTKLDTTWEGVPIQ
                     HVLELAQLRPSVSHVVAHSEQGYTANLPLSVLDDDDVLLADTFDGQPLELEHGYPLRL
                     LVPKRYFWKSAKWIRGLEFLDHDQLGFWERYGYNNDADPWKEERYSE"
     gene            complement(4400..5092)
                     /locus_tag="VIF63_08605"
     CDS             complement(4400..5092)
                     /locus_tag="VIF63_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239482.1"
                     /translation="MSLGGALRQSLRDFFFNSWRLAPANLVWGLGFGAMLVAGPPTVI
                     GVVLLVLLAIPLAGLHRMAALVARDEPADFSDFVSGMRRYAAPAVVVAGGAAILAGVF
                     TTNIVVGFEAGGPVGWFVSAMALWGNVALAMFLVAFWPILADPRREGLGLRRRLGLAG
                     LVVIGRPVRLFVLTAAVALILLISTVLFVAILLVSVAYVSLVASRTVLPMLDALEARL
                     PENRVPETRIPG"
     gene            complement(5096..6094)
                     /locus_tag="VIF63_08610"
     CDS             complement(5096..6094)
                     /locus_tag="VIF63_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8239483.1"
                     /translation="MRDPESARSSAEPLRKPSSVAAAVGGGEAGIVAALPPPGRPGSG
                     ADAGAVAVGDKTRGLRLKIAYALMISYALLMFVPFAWTVITSLKTRADSLRLTVIPQP
                     VSVDGWRYMLETLDPSIFTLFTNSVMLAAAVTVTNLVLGSLGGYAFARLRFPGREILF
                     LVVLATLMIPDPLRLVPMYVMFTELGLTRGVGQYVSIILVLGISATSIFLLRQYFLSI
                     PKDLEEAARMDGAGFFTTFLKVMLPLAGPALAAVAILQFQGAWNSFFWPVILLRDPEH
                     YTIPQALYLFREAGGFSTNWPPLMAVITIATLPILFLYVFFQRYFVEGVAASGVKG"
     gene            complement(6094..>6284)
                     /locus_tag="VIF63_08615"
     CDS             complement(6094..>6284)
                     /locus_tag="VIF63_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239484.1"
                     /translation="LIAGSSNGDPQYALMTVVLYLYNAAFAQLDPGYAAAVGAVLFLV
                     IFGATLVQRRLFGNTEAA"
BASE COUNT         1034 a         2117 c         2223 g          910 t
ORIGIN      
        1 gcggcgtaga tcacctcgat gctgtaggtc cggtcgtcga gtcggaccga gaaggtccgg
       61 cggtgctgca tcacgcagcg atcctaccgc ggggctaggc cgcctcgatc gagcggaggg
      121 cgtgggcgag ggcctcgtgg aggctgttgt cgaggccggt cccggcaacg ctgacccgaa
      181 cggtgacggg cgcgccggtg cgctggatcg cttcgcggca ggtggcggca acccgcgcgg
      241 ccagctgttc ggcgcccgtt tcgggcgtct ccggcaggag catctggaag cgcgtgctcg
      301 cgacgcgcgc aacgaggtcg gtggcgcgcg agtcctggcg gaggctgcgc gcgatcgggc
      361 cggccagggc gcccagccac ggatcgaccg attgcccgtc gcgctcggcc ttcagctcga
      421 tgatcgcgac cgacgcgggc cgctggtagc gagccgcccg cacgctctcg cgttcgagcg
      481 cggcgttcca ttcgagccgt ccgggcaggc tccggaggtc ggtcgagggc cgggcgggag
      541 cggtggggcg atgggccggc gtggtcgtgg tcgagtcgac cgtggggtcg ctctccgcga
      601 gcgtcgccgg tcgtccgcgg cggacgtcgg gtccctcgag gaacgcctcg atcgactcgc
      661 cggtgcgctc gtcatcgagc gccgggagcg cgaatcggcg tgctcgtgtc gactcggacg
      721 tggccatggg cctccgggtt gggtggtggg ggtggcacgt caaccatagc ccagcgtccc
      781 gaaatcgtca gtcgcgccgc cgcgcagcca cgccgatcgc ctgcgatccg ggtcccgcgc
      841 tcgccggccg cgctgattgt ccgcgtggag gacgggcggg acaaactcgt cgctggaatc
      901 tgcgaatagg cttcgggatc aggctcaatg tccgcgtgga ggacgagtgg gaccgaaatg
      961 gcgctctggc gcgcggtttg ggctcgcact gcatccgatt cgtcctcgga gcggacaaac
     1021 agcctcgaat ctgggtcgtg agaccctctc ggacccaatt cctgttcgag ttgtcctcgg
     1081 agcggacatc cggcttggag ctacgggcgc gccggcgagg tgggccgcgt gcgtgcgcca
     1141 cgtgggccag gacgatcgat ttcgctgcgg gtggttgtcg gaatcggttg cgctgcgtac
     1201 gattgcagca gatcgcaaac cacaagcgga aaccgcgcgc ctcgaagggg taccgaaccg
     1261 atgatcgaac agcatccagt cgtcgagccg cagccgatcg tcgacacgac ggtgctctcc
     1321 ctcagcgacg ccgcggcaac gaagctgcgc gagctgacga aggaagagac caacccgaac
     1381 atcgggctgc gtgtctacgt gtactccggc ggctgctcgg gcttccgcta cggcatgatg
     1441 ctcgaggacc agccgactcc cgaggacaac atcctcgagg tcaacggcac gaaggtgtac
     1501 gtcgacacca acagcattcc gctcctgaag ggctccgaga tcgactacgt cgacaccctc
     1561 atgggcgccg gcttcacggt caacaacccg aacgcggtca cggcctgcgg ctgcggttcg
     1621 agcttccgga ccgcggacga ctccggaacc gcccgctcct gcgcgcacta ggacctcgtc
     1681 ggtagcgcga agcgatcgcg cgattcgcat tgagctgtcc gagggctgag gcgacgcccc
     1741 tgcgagcaat cagcccgagg attcaaccgg cggcttcttg attcccggca actcgtcggc
     1801 cgtccgacgc ggattcgggc cgatcgcggg aatggcccga acctgacgca tatttgcttg
     1861 attcgcccca gccgggtcgc gaccctgccc ggatgaccat cgccagcccg ccgaccgcgc
     1921 tcgaggccgc cctgcggcag gtggacgaaa ttgccgacaa gacgtttgcc ggctggcacg
     1981 tccccgggct cagctacggg gtggtcctgg gcgaccggct gatccatgcg cgcgggctgg
     2041 gcacgctccg cgtcggcgag gatgcgacac cgacgccgtc atccgtcttc cgcatcgcct
     2101 cgatgaccaa gagcttcacc gcggcgaccg tgctgtcgct ccgggacgac aagctcctcg
     2161 ccctcgacga tccgatcgcg cgctacgtcc ctgagctcgc cgaccttcgc tacccgacga
     2221 ccgactcgcc gcccatcacg atccgccacc tgctgacgat gaccgccggc ttcccgaccg
     2281 acgacccgtg gggcgatcgc cagcaggcgc tcgacctcgc tgcgttctcg cgcctgctct
     2341 cgaacggcct gtcgttcgcc tgggcgccgg gcacgcgctt cgagtactcg aacaccggct
     2401 acggaatcct cgggcggctg atcacgaacg tcgtcgggcg tgagtacaag gacgtggtcc
     2461 gcgaccgcat gcttcgcccc ctcggcatga acgccaccgg ctacgaggag tcggaggtac
     2521 ccgaggaacg aaaggcgatg ggctacgtct ggcgcgacgg cacctacctg cccgagggca
     2581 tggacccgta cggcgccctc gcggcgatgg gcgggatctt cacctcggtc gaggacctcg
     2641 cgacatgggt gagcggcttc atcgatgccg tgccgccgcg cgacggcccg gacatctcgc
     2701 atccgctgcc gcgcgccacg cggcgcgaga tgcagcagcc gatggtgccc acgggcctgc
     2761 gcacgacgca cacctcggcc gacgcagtca ccgaggtcga ggccggtgcc tacggctacg
     2821 ggctgttcac cgtcgacgac gttcgctacg ggcagacggt cgggcacagc ggcggctatc
     2881 ccggcttcgg ctcgaacatg cgctggcatc aagcgtcggg tctcggcgtg atcgccttga
     2941 cgaatcaccg gtacggtgcg gcgacgctgc tcacgcggga gctcctcaac gagctcatcc
     3001 gggccgatgc agtcccagtc cgccgggtgc gcccgaacgc cgcgacggag gcggcccgcg
     3061 aggcggtcga aggcctgatc ggcgcctggg atgacgacac cgcggccgcc ctcctggcga
     3121 tgaatgtcga cctcgacgaa ccgatcgggt cgcggcgcgc gttcgtcgaa cggctgcgcg
     3181 cacgccacgg cgccctccgg cgtgacgaat ccgagcccgt cgaatcgctg acgtccttcc
     3241 acgccatctg gtggctggct ggagagcgcg gccgcgtgcg cgtcgagatc ctcctgtcgc
     3301 ccgagctgcc gccgaaggtc cagacgttcg gactgacctc ggtgccggaa ccgccggccc
     3361 gtctccgcca cgccgccgaa cggctgctcg gcgtcatgca acccacggac tccggcccgg
     3421 tggccatcga ctggccggcc gacctcacgg tcggcacgac ggtcgacgtc ggtgcagtcg
     3481 tgcgggcgat gcgcgccacg gaggcgcgat tcggaccgat cagcctgggg ccgccgattg
     3541 caggcgacgg cgagaccaag gtcacgtacc gactcatctc accgcgcggt cgcgtggatc
     3601 tcgccctcga gcttgactcg atcgccgatt gcctgtccgc cgtgtcgctc gtgcccgcga
     3661 agcaggtccc gccagacctc gactgacgat cccggcgccc gagttcggca ggacgctatt
     3721 cggagtagcg ctcttccttc cagggatcgg cgtcgttgtt gtagccgtag cgctcccaga
     3781 agccgagctg gtcgtggtcg aggaactcga ggccgcggat ccacttcgcg ctcttccaga
     3841 agtagcgctt cgggacgagc agccgcagcg ggtagccgtg ctcgagctcg agcggttggc
     3901 catcgaacgt gtcggcgagc agcacgtcgt cgtcatcgag gaccgacagc ggcaggttcg
     3961 cggtgtagcc ctgctcggag tgcgcgacga cgtggctgac gctcgggcgc agctgggcaa
     4021 gctcgaggac gtgctggatg gggacgccct cccaggtcgt gtcgagcttc gtccagcggg
     4081 tcacgcagtg gatgtccgtc gcgacggact tgcgcggcag cgccttgaac tggtgccagt
     4141 tgagcgtgaa cggcgaatcg acctggccgt agaccttgaa gtcccaggtc gcgaggtcgg
     4201 tcctgggcac ggagccgtag tgcaggaccg ggaacttctc ggtccggtac tggccgggcg
     4261 ggatgcgatc ggcgaccgcc gcgtcccgct ccttgttgcc gaagagtcgc tcgtacaacg
     4321 ctgctgcctc gggcctcatg gggatgcacg catggtaagc gtgaacgatc ggcgaccgag
     4381 gtgacgcccg gatgacgcct cagcccggga tgcgggtctc cgggacgcgg ttctccggaa
     4441 ggcgcgcctc gagggcgtcg agcatgggca ggacggtgcg cgacgcgacg aggctgacgt
     4501 aggcgacgct cacgagcagg atcgcgacga agagcaccgt cgagatcagc aggatcaggg
     4561 cgacggccgc ggtgagcacg aagaggcgga ccggccggcc gatgaccacc agtccggcga
     4621 gtccgaggcg gcgccgcagg ccgaggccct ctcggcgggg atcggccagg atcggccaga
     4681 aggcgacgag gaacatggcc agcgccacgt ttccccagag ggccatggca ctgacgaacc
     4741 agccgaccgg tccccccgcc tcgaacccga cgacgatgtt cgtcgtgaac acgccggcga
     4801 gaatcgccgc tcctccggcc acgacgaccg cgggcgcggc gtaccggcgc atcccgctca
     4861 cgaagtcgga gaaatcggcc ggctcgtcgc gggcgacgag cgcagccatc cggtgcaggc
     4921 cggccagggg aatcgcgagg agcacgagga gcacgacgcc gatgacggta ggcgggccgg
     4981 cgacaagcat cgctccgaag ccgaggcccc acacgagatt cgccggcgcg aggcgccacg
     5041 agttgaagaa gaagtcccgc agcgactggc ggagcgcgcc tccgagcgac atcggctagc
     5101 cctttacgcc cgaggccgcg acgccttcga cgaagtagcg ctggaagaag acgtacagga
     5161 acaggatcgg cagggtcgca atggtgatga ccgccatgag cggcggccag ttcgtcgaga
     5221 acccacctgc ctcgcggaag aggtacagcg cctgcgggat cgtgtagtgc tccgggtcgc
     5281 ggagcaggat caccggccag aagaacgagt tccaggcacc ctggaactgg aggatcgcca
     5341 ccgcggcgag tgccggcccc gcgagcggca gcatcacctt caggaacgtg gtgaagaacc
     5401 cggcgccgtc catccgggcc gcttcctcga ggtccttggg aatggacagg aaatactgtc
     5461 gaagcaggaa gatgctcgtc gccgagatgc cgaggacgag gatgatgctc acgtactggc
     5521 caaccccgcg cgtcagcccg agctccgtga acatcacgta catcggcacg agccgaagcg
     5581 ggtccgggat catcagggtg gccaggacca cgaggaacag gatctcgcgc cccgggaacc
     5641 gcagccgcgc aaacgcgtac ccgccgagcg agccgaggac gaggttcgtg accgtgacgg
     5701 cggcggcgag catgacgctg ttcgtgaaca gggtgaagat gctcggatcg agggtctcga
     5761 gcatgtatcg ccagccatcg accgagacgg gctgcgggat gaccgtcaag cggagcgaat
     5821 ccgctcgcgt cttgagcgac gtgatcaccg tccaggcgaa cggcacgaac atcagcaggg
     5881 cgtagctgat catcagggca taggcgatct tgagccggag ccctcgcgtc ttgtccccga
     5941 ccgcgacggc gcctgcgtcc gcgccgctgc ccggcctgcc tggcggcggc agggccgcca
     6001 cgattcccgc ctcgccgccg ccgaccgcag cggcaaccga gctcggcttg cggaggggct
     6061 cagccgagga gcgagcggac tcggggtctc gcatcacgcg gcctcggtat tgccgaagag
     6121 tcggcgctgg acgagcgtcg cgccgaagat caccaggaag agcacggccc cgaccgccgc
     6181 ggcgtagccc ggatcgagct gggcgaaggc ggcgttgtac aggtacagca cgacggtcat
     6241 gagggcgtac tgcggatcac cgttggagct gccggcgatc aacg
//
LOCUS       DATBSW010000314         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_347476, whole genome shotgun sequence.
ACCESSION   DATBSW010000314 DATBSW010000000
VERSION     DATBSW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347476"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..287)
                     /locus_tag="VIF63_08620"
     tRNA            complement(213..287)
                     /locus_tag="VIF63_08620"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Gln,seq:ctg)
     gene            440..877
                     /locus_tag="VIF63_08625"
     CDS             440..877
                     /locus_tag="VIF63_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY8239485.1"
                     /translation="MALKPKRPESSIRVGQAAEMLQVSVETLRRWEVDGRLRTDRTSG
                     GQRMVPIGEITRLLAERRAQATDRPAVAGSARNRFPGIVTRVEKDRVAAVVEVIAGPH
                     RMVSLMTAEAVDELDLRVGDEAVCVVKATNVIVEKPFRKDARS"
     gene            904..>1591
                     /gene="modA"
                     /locus_tag="VIF63_08630"
     CDS             904..>1591
                     /gene="modA"
                     /locus_tag="VIF63_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8239486.1"
                     /translation="MALTAALSVLLGACGGQTASPSPPITSCCDPTAPPVELTIYAAA
                     SLKGALEEVKSRYEALSEVVTLVISTDSSAALEAKIEQGAPADVFLSADTANPQKLVD
                     GGFATEPVMTFASNKLTVIVPTDNPGGLASPRDLIKPGVKVIAAGPTVPITTYARQLL
                     ENLAKENGYPADFPTQYAANVMSEEENVAAVVAKIELGQGDAAIVYVTDAATSTGVQS
                     IEISPSSNVAA"
BASE COUNT          263 a          538 c          529 g          261 t
ORIGIN      
        1 ctcgatccag tgtttccgtt cgggccgcga tagcgccgag tccctgcacc tccggttcgc
       61 catcgctgac accaacgaag gggcggtggt gtggggcagc gggccatcgg tcgcgcctcc
      121 tcgaccgaag gctacggctc aacacgccag gcacccttgc cgaacggccc ttcacgaggg
      181 tccactcgca ggaattcgtg gatcggtgta gttggctggc gaggaaggat tcgaaccttc
      241 aatcccctga tccagagtca ggtgccttac cgtttggcca ctcgccagcg gcgctgcggg
      301 attttacccc cgcactcggg tgcggtcgag gaaccgatcg ttcgccctcg gtccaacctc
      361 gcgccctggg tgagcatgtc taggcgaact cgatacaatc cctgcgattt tcaccgatcg
      421 aagggatgga tatcgcggta tggccctgaa accgaagcgc ccggagtcgt cgatccgcgt
      481 cggtcaggcg gccgagatgc tgcaggtgtc ggtcgagacg ctccgtcgct gggaggttga
      541 cggcaggctg cgcaccgatc gcacgagcgg cggccagcgg atggtgccga tcggcgagat
      601 cacgcggctg ctcgccgagc gtcgcgccca ggcgaccgac cgaccggccg ttgccgggtc
      661 ggctcggaac cgctttccgg gcatcgtcac gcgcgtcgag aaggaccgcg tcgcggcggt
      721 cgtcgaggtc atcgccggcc cgcatcggat ggtcagcctc atgaccgccg aggcggtcga
      781 cgaacttgac ttgcgggtcg gcgacgaggc ggtgtgcgtc gtgaaggcaa ccaacgtcat
      841 cgtcgagaag ccgttccgga aggacgcgcg ctcgtgaagg tatccggcct tgccgctcgc
      901 cttatggctc tcaccgcggc gctgtccgtc ctcctcggtg cttgcggagg ccagacggct
      961 tccccgagcc cgccgatcac ttcctgctgt gacccgaccg cgccacctgt cgagctgacg
     1021 atctacgccg cggcgtcgct taagggcgcg ctcgaggagg tcaaatccag gtacgaggcg
     1081 ctgagtgagg tcgtgaccct cgtgatctcg accgattcgt ccgccgcact cgaggcgaag
     1141 atcgagcagg gcgctccggc cgacgtcttc ctgtcggccg acaccgcgaa cccgcagaag
     1201 ctggtcgacg gcggcttcgc aacggagccc gtcatgacgt tcgccagcaa caagctgacg
     1261 gtgatcgtcc cgaccgacaa cccgggcggc ctcgccagcc cgcgcgacct catcaagccg
     1321 ggcgtgaagg tgatcgccgc cgggccgacc gtcccgatca cgacgtacgc ccgccagctt
     1381 ctcgagaacc tcgcgaagga gaacggctac ccagcggatt tcccgacgca gtacgcggcc
     1441 aacgtcatgt cggaggagga gaacgtcgcc gcggtcgtgg ccaagatcga gcttggccag
     1501 ggcgacgcgg cgatcgtgta cgtcaccgat gcggcgacgt cgaccggcgt gcagtcgatc
     1561 gagatctccc cgtcctcgaa cgttgccgcc a
//
LOCUS       DATBSW010000315         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_311608, whole genome shotgun sequence.
ACCESSION   DATBSW010000315 DATBSW010000000
VERSION     DATBSW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311608"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..710)
                     /gene="rplI"
                     /locus_tag="VIF63_08635"
     CDS             complement(48..710)
                     /gene="rplI"
                     /locus_tag="VIF63_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8239487.1"
                     /translation="MEVILLTDVEKIGLRGDVVNVARGYARNFLLPRRLAEEATPAKV
                     NELRKREEQRARHEAQTFEQAREIADRLGQTELRFDVKAGPTGALFGSVTPTDIVDEL
                     WKQQKIRVDRRKVGIDNIKRIGRHTVPIELFNDVDVEVRALVVPEGGELPPEEELEAM
                     EAAAVAEEEAAAAAAAAQHAEVQEFLADDEPEAEPAATDEPEPAAEREAEPVAAEPES
                     QQ"
     gene            complement(711..980)
                     /gene="rpsR"
                     /locus_tag="VIF63_08640"
     CDS             complement(711..980)
                     /gene="rpsR"
                     /locus_tag="VIF63_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY8239488.1"
                     /translation="MPKPKEKEQRGRKRPPMGGPQRKRNCYFCKDKIDEVDYKNINQL
                     RRYISEKGKIRSRRITGACRRHQNQVSVAVKRAREMALLPYVGER"
     gene            complement(980..1420)
                     /gene="ssb"
                     /locus_tag="VIF63_08645"
     CDS             complement(980..1420)
                     /gene="ssb"
                     /locus_tag="VIF63_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8239489.1"
                     /translation="MANINRVILVGNLTRDPELRHTPSGTSVCKLRLAVNSRQKDAAT
                     GEWGEKPNYFDVTVWGNQGENCAKFLSKGRPVGVDGRLDWREWEAQDGTKRQAVEVIA
                     DTVQFLGSREGGGAAGGDGEHQFVPAGAKQDDFAPTGADDDIPF"
     gene            complement(1433..>1833)
                     /gene="rpsF"
                     /locus_tag="VIF63_08650"
     CDS             complement(1433..>1833)
                     /gene="rpsF"
                     /locus_tag="VIF63_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8239490.1"
                     /translation="DQEKRRYRELRTEYEILLMLDPELPDGRSDEILQRARELVEKSG
                     GSWDRHEPWGRRKLAYEITHKDEGVYHLFELTAEPATLDELTRILKITDGVMRHMAVR
                     RVQRSPGRPVAPPPRDVQDEPEPIAASVDE"
BASE COUNT          219 a          655 c          591 g          368 t
ORIGIN      
        1 agttcaagag aaagtccaca ggcagaagcg cacctgtgga cgagtcgtta ctgctgcgac
       61 tcgggctcgg cggcgaccgg ctcggcctcg cgttccgcgg ccggttccgg ctcgtcggtc
      121 gcggccggct cggcctcggg ctcgtcgtcg gcgagaaact cctgcacctc ggcgtgttgt
      181 gctgcggcgg cggcagcggc ggcctcttcc tccgcgactg ctgcggcctc catcgcctcg
      241 agttcctcct ccggcggcag ctcgccgccc tcgggcacga cgagcgcgcg cacctcgacg
      301 tccacgtcgt tgaagagctc gatcggcacg gtgtggcgcc cgatccgctt gatgttgtcg
      361 atcccgacct tgcggcggtc gacgcggatc ttctgctgct tccagagctc atccactatg
      421 tcagtaggtg tgaccgagcc gaagagcgcg ccggtcggtc ccgccttgac gtcgaagcgc
      481 agttcggtct ggccgagccg gtcggcgatc tctcgggcct gctcgaaggt ctgcgcctcg
      541 tgccgcgctc gctgttcttc gcgcttgcgg agctcgttca ccttggcggg cgtcgcctcc
      601 tcggcgagcc ggcgcggcag caggaagttc cgcgcatagc cgcgcgcgac gttgaccacg
      661 tcgccgcgca ggccgatctt ctcgacgtcg gtgaggagga tgacttccat ctagcgttcg
      721 cccacgtacg gcaggagcgc catctcccgc gcgcgcttca ccgcgaccga gacctggttc
      781 tgatgcctgc ggcacgcgcc ggtgatccgc cgagagcgga tctttccctt ctcggagatg
      841 tagcgccgca gctggttgat gttcttgtag tcgacttcgt cgatcttgtc cttgcagaag
      901 tagcaattgc gcttccgctg cggcccgccc atcggcggcc gcttgcgccc tcgctgctcc
      961 ttttccttgg gcttcggcat cagaacggga tgtcgtcgtc ggcgccggtg ggtgcgaagt
     1021 cgtcctgctt ggcaccggcg ggcacgaact ggtgctcacc gtcgccgccg gcggcgccac
     1081 cgccctcacg gctgccgagg aactggaccg tgtcggcgat cacctcgacc gcctgtcgct
     1141 tggtgccgtc ttgtgcttcc cactcgcgcc agtccagccg gccgtcgacc ccgacgggcc
     1201 gacccttcga gaggaacttc gcgcagttct cgccctggtt tccccagacg gtgacgtcga
     1261 agtagttcgg cttctcgccc cactcgccgg tcgccgcgtc cttctggcgg ctgttcaccg
     1321 cgaggcgcag cttgcacacc gacgttccgc tcggcgtgtg ccgtagctcg gggtcgcgcg
     1381 tgagattgcc gaccaggatg acccggttga tgttcgccat gctctcctcc tcctactcgt
     1441 ccacggacgc cgcgatcggc tccggctcat cctgcacgtc tcgcggcggc ggcgctaccg
     1501 gccgtccggg actgcgctgc acgcgccgga ccgccatgtg tcgcatcacg ccgtcggtga
     1561 tcttgaggat gcgcgtgagc tcgtcgaggg tcgccggctc ggcggtcagc tcgaacaggt
     1621 ggtagacgcc ctcgtccttg tgcgtgatct cgtacgcgag cttgcgccgg ccccagggct
     1681 cgtgccggtc ccagctaccg ccgctcttct cgacgagctc gcgggcgcgc tggaggatct
     1741 cgtcggaccg accgtccggc agctccggat ccagcatgag cagaatctcg tactcggttc
     1801 tcaactcccg atacctcctc ttctcttggt ctt
//
LOCUS       DATBSW010000316         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_488419, whole genome shotgun sequence.
ACCESSION   DATBSW010000316 DATBSW010000000
VERSION     DATBSW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488419"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VIF63_08655"
     CDS             <1..323
                     /locus_tag="VIF63_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239491.1"
                     /translation="VGRLADAYHASAANPTTVAARVPIIRAAAEEAGRPMPRLSARAR
                     LELGAEQESFYTMHGSAAQVASEIRAFADVGVDHLALAFPPRDAAGLTDAVERFRNEV
                     LPLV"
     gene            590..961
                     /locus_tag="VIF63_08660"
     CDS             590..961
                     /locus_tag="VIF63_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239492.1"
                     /translation="MATPAATAAPTAFPTATPEPAPIAITDVECLTGLTVRFHVHIAT
                     EQAITMYQLWSTWGGGGNSDVTLSPPLPHAIDEDVEFTHTQIDPVENRIHEFGLAVTV
                     EGATDPIIVYAFEPEFRCPGH"
     gene            complement(962..>2908)
                     /gene="ligD"
                     /locus_tag="VIF63_08665"
     CDS             complement(962..>2908)
                     /gene="ligD"
                     /locus_tag="VIF63_08665"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY8239493.1"
                     /translation="RNGNDAETYFPKLLTKATWIDAREAIVDGEVVAIGEDGLPDFSL
                     LQEATGGASTRFVYQAFDLLHLDGRSLVGVPLESRKQLLRSILRGDDRRVRFADHVVG
                     EGVAFHEAAKAQGLEGVIAKHRRSIYEPGKRSRTWLKIKIRPEQELVVGGWTPGEGNA
                     KELGALVVGYYEKEGRAKKLRFAGKVGSGFTAATRKRLRERLKDLETDDSPFDPPPPK
                     DYKGRWGGELKYVVWVKPELVIRAELGGWTRDGIVRQAAFKGFDEGGKKATLVVREVA
                     VAAGATVKAAEAEVDQMAEDAPVPTKKAPSASAAKATSNAKSNAKATPAASTAKHEFP
                     WSATKDELAALDAMTKDGSWSVGGFELKLTNLDKVIFPPRDEAPVTKRDLVRYFAQIA
                     PVMLPHLQERALNFQRFPNGPNAPGFWQKDIPGTAPSWLRRWKEVGVEEREANDHLIA
                     DRVGALCWLGNQTSFEIHAWTSRIEDPMTPTFALIDIDPGTDTTWDETLTLARLYRTA
                     LGHLGVRGYPKTTGKRGIQIWIPVDLKYTFRETSDWVETLSRAVGSTVPNLVSWEWAK
                     NRRGGKARLDYTQNTYIKTLVAPYAVRPADGAPVSTPITWDELDDPELRSDRWTIRTV
                     VERVGKVGDLFAKAQTDAQELPAI"
BASE COUNT          408 a         1064 c          946 g          490 t
ORIGIN      
        1 gcgtcgggcg gctcgccgac gcgtaccacg cgtccgcggc gaatcccacg accgtggcgg
       61 cgcgcgtgcc gatcatccgt gccgctgccg aggaggccgg ccggccgatg ccccgcctct
      121 ccgcccgcgc ccgcctggag ctcggcgccg agcaggagtc gttctacacg atgcacggct
      181 cggcagcgca ggtcgcgagc gagattcgcg cctttgccga cgtcggcgtc gaccatctcg
      241 cactcgcgtt cccgccgcgt gatgcggcag gcctcacgga tgcggtcgag cgcttccgca
      301 acgaggtcct accgctcgtc tgaccatcgg cccgtcgcac gcgaccggcc gggataggat
      361 cgttccagtc atcaatccgc ttcgggggaa ctgtgcgttg cgagccaggt cgaatctcgc
      421 gctcctcacc acgctgacgg tcgccatggg gctcatcgcc gcgtgcagta gcccagggtc
      481 atcgccggtc gacaccgcgg gtctgccagt cgtgaccgcg tcacccgcac cagcgacgcc
      541 cggggtgacg cccgtcgcga cggccgcgcc cgtggcgacg ctcacacctg tggcaacccc
      601 cgccgccacg gccgcgccga ccgcgttccc gacggccacc cccgagcccg cgccgatcgc
      661 catcaccgac gtcgagtgcc tcaccgggct gaccgtccgc ttccatgtcc atatcgcaac
      721 cgagcaggcc atcacgatgt accagctgtg gtcgacgtgg ggcggcggcg gcaacagcga
      781 cgtgaccttg agcccgccgc tgccacacgc gatcgacgag gacgtcgagt tcacccacac
      841 gcagatcgac ccggtggaaa accgcatcca cgagttcgga ctcgcggtga ccgtcgaagg
      901 cgccaccgat ccgatcatcg tgtacgcctt cgagcccgag ttccgctgcc cgggccactg
      961 atcagatcgc gggcagctcc tgggcgtcgg tctgcgcctt cgcgaacagg tcgccgacct
     1021 tcccgacccg ctcgaccacg gttcggatgg tccatcgatc gctgcgcagc tcggggtcgt
     1081 cgagctcgtc ccaggtgatc ggggtcgaga cgggcgcgcc atccgcgggc cgcacggcat
     1141 acggcgcgac gagcgtcttg atgtaggtgt tctgggtgta gtcgaggcgc gccttgccgc
     1201 cgcgccggtt cttcgcccac tcccacgaga cgaggttcgg cacggtcgac ccgactgccc
     1261 gcgagagcgt ctcgacccag tcgctcgtct cgcggaacgt gtacttcaag tcgaccggga
     1321 tccagatctg gatgccgcgc ttgccggtcg tcttcgggta gccgcgcacg cccaggtgcc
     1381 cgagtgccgt ccggtacagg cgggccaggg tgagggtctc atcccacgtc gtgtcggtac
     1441 ccgggtcgat gtcgatcagg gcgaacgtgg gggtcatcgg gtcctcgatg cgcgacgtcc
     1501 atgcgtggat ctcgaacgac gtctggttgc ccagccagca cagcgccccg acccggtccg
     1561 cgatcaggtg atcgttcgct tcgcgttcct cgacgccgac ctccttccag cggcgcagcc
     1621 aagacggcgc ggtgcccggg atgtccttct gccagaagcc gggcgcgttg gggccgttcg
     1681 ggaagcgctg gaagttcagg gcgcgctcct ggaggtgcgg cagcatcacc ggcgcgatct
     1741 gggcgaagta ccgaacgagg tcccgcttcg tgacgggagc ctcgtcccga ggcgggaaga
     1801 tcaccttgtc gaggttcgtg agcttgagct cgaacccgcc gacggaccag ctcccgtcct
     1861 tcgtcattgc gtcgagggcc gcgagctcgt cctttgtcgc cgaccacggg aactcgtgct
     1921 tcgccgtcga agccgcggga gtcgccttcg cgttcgactt cgcgttcgac gtcgccttcg
     1981 cagctgacgc gctgggagcc ttcttcgtcg gcacgggagc atcttccgcc atctggtcga
     2041 cttcggcctc ggccgccttg accgtcgctc ccgccgcgac tgccacctcc cgcacgacga
     2101 gggtcgcttt cttgccgccc tcgtcgaagc ccttgaacgc ggcctggcgg acgatcccgt
     2161 ctcgcgtcca gccgccgagc tccgcgcgaa tcacgagctc gggcttcacc cacacgacgt
     2221 acttgagctc accgccccag cggcccttgt agtccttcgg gggcggcgga tcgaatggcg
     2281 agtcgtcggt ctcgaggtcc ttgagccgct cgcgcagtcg cttccgcgtc gcggccgtga
     2341 acccggagcc gaccttcccg gcaaatcgaa gcttcttcgc tcgcccttcc ttctcgtagt
     2401 agccgacaac cagcgccccg agctccttcg cattgccctc gcccggcgtc cagcccccga
     2461 cgacgagctc ctgctccggc cggatcttga tcttgagcca cgttcgcgag cgcttgcccg
     2521 gctcgtagat agatcgccgg tgcttcgcga tgacgccctc gaggccctgt gccttggccg
     2581 cctcgtggaa cgcgacgccc tcgccgacga cgtggtccgc aaatcggacg cgtcgatcgt
     2641 caccgcgcag gatcgatcgc aggagctgct tccggctctc gagggggacg ccgaccagcg
     2701 atcgaccatc gaggtggagc aggtcgaacg cctggtagac gaaccgcgtc gaggcgccgc
     2761 cggtggcctc ctggagcagg ctgaagtcgg ggagcccgtc ctcgccgatc gcaacgacct
     2821 cgccgtcgac gatcgcctcg cgcgcatcga tccaggtcgc cttcgtgagc agcttcggga
     2881 agtaggtctc ggcgtcattg ccgttgcg
//
LOCUS       DATBSW010000317         4143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_630044, whole genome shotgun sequence.
ACCESSION   DATBSW010000317 DATBSW010000000
VERSION     DATBSW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630044"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VIF63_08670"
     CDS             complement(<1..770)
                     /locus_tag="VIF63_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239494.1"
                     /translation="MAHRIQMKLGVVPDPERAPDSPDTALHVEPRVGAQTRGKGHLYL
                     LVTSRVPGPRAREATRLVADSIRTEYYYDESAGIRVCLVKAIQVANKRLTHARERGAL
                     GNGPGPIGVALAVVRDNELYVCTVGPAEAYLNRGARLSTLPDPHRDRGLPSTEVEPEV
                     WRGEINVGDQLLLVSPTIVEALGADALKDAMVQLHPQSAVEALTTRFRAGGGTGSDGA
                     LVIEAAEIAVTRGGVVPVPVRPAEPLAGAPDRSPIPLA"
     gene            complement(842..1180)
                     /locus_tag="VIF63_08675"
     CDS             complement(842..1180)
                     /locus_tag="VIF63_08675"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY8239495.1"
                     /translation="MSDDPEVQRKRRRPMSRIGDLMPQAAAHLGLQDELRRARFLATF
                     DAIVAELTPAAHGACRALKVDGETLVVEADAPIVGQELVLHGSELAAAFSRAPGGAQV
                     RQVRVTVRRR"
     gene            complement(1177..2364)
                     /locus_tag="VIF63_08680"
     CDS             complement(1177..2364)
                     /locus_tag="VIF63_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY8239496.1"
                     /translation="MGPGPGAAGSTSAPVATIDRLRLADLRAYATLEAEFGGGPQLVW
                     GPNAAGKTSLLEAMVVLARGSSHRTTTDAELVRWGADLARIEGRSSGDEIEVALVRPG
                     SAAAAGGARKRIRVNGVPRRAAVLAERLRVVLFAPEDMLLVAGSPSLRRSTLDQLATT
                     LVPGYAAELATYARALQQRNGLLRAIRDETASRDELRYWDQPFLDAGGAVVAARHELL
                     ARLAGPLAAAHAEIAPEEAAAGALGAEYVTNAPAGPDETPRDALKRRLAETAEKEMWN
                     GTTLVGPHRDDVAFVMAGRDLSAFASRGQQRTAILALKLAELDLVTSHDSRPPLLLLD
                     DVFSELDPARRGHLVRRIAELPQAFVTTTTLDDLDPGLRAIATPWEVRADLDGARLLS
                     SAS"
     gene            complement(2370..3488)
                     /gene="dnaN"
                     /locus_tag="VIF63_08685"
     CDS             complement(2370..3488)
                     /gene="dnaN"
                     /locus_tag="VIF63_08685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY8239497.1"
                     /translation="MKLSVMQENLARGLGVVSRAVSNRSTLPVLANVLLKTEDGGLRL
                     TTTNLEIGLVYWVPGKVETDGATTVPARLLSDLVSSLSGGDRIELESGAGETLHLKSG
                     RFESHVKGIDADEFPSIQPTGERPTTRIAQNVLKRALEETAFAAATDEARPILTGVLW
                     RFEGETVTLAAADNYRIAVKTIPVLDPVGDTSVVVPARALIELMRVLADSDDPVEIVL
                     AQSRNQILFHLEGIDLVSRLIDGQFPNYQAVLPKAHTTRAILDREELLRAVRPAALIA
                     HESANIVKLLISANGESGITVSANAEIGDHVGQVEAKVEGDGTSIAFNAKYLADVLEK
                     VSAEQFALELQGPLAPGVFKPVGDDQYVHVVMPVRTTS"
     gene            complement(3916..>4143)
                     /locus_tag="VIF63_08690"
     CDS             complement(3916..>4143)
                     /locus_tag="VIF63_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8239498.1"
                     /translation="LKGQKRDKAIVVPRQIAMFLMREETDVSLLRIGAELGNRDHSTV
                     LHACDKITRESGGNDELRREIAAVRELIYAD"
BASE COUNT          562 a         1448 c         1511 g          622 t
ORIGIN      
        1 tccgcgagcg ggatcggcga gcggtccggc gccccggcca ggggctcggc cggtcgcacg
       61 ggcaccggca cgacaccgcc gcgagtcacg gcgatctccg cggcctcgat gaccagcgcg
      121 ccgtcgctgc ccgtcccgcc gccggcccgg aagcgggtcg tgagcgcttc aactgccgac
      181 tggggatgga gctggaccat ggcgtccttc agcgcgtccg cgccgagcgc ctcgacgatc
      241 gtgggcgaaa cgagcagcag ctggtcaccc acgttgatct cgccgcgcca gacctcgggc
      301 tcgacctcgg tcgacgggag gccccggtcg cgatgcggat ctggcagggt cgagagtcgc
      361 gcgccgcggt tgaggtacgc ctcggcaggc ccgaccgtgc acacgtacag ctcgttgtcg
      421 cggacgaccg cgagcgcgac cccgatcgga cccggcccgt tgccgagcgc accgcgctcg
      481 cgggcatggg tcagtcgctt gttcgcgacc tggatcgcct tgacgaggca gacgcgaatc
      541 cccgccgatt cgtcgtagta gtactcggtg cggatcgagt cggcgacgag ccgggtcgcc
      601 tcacgggccc gcgggccggg gacgcgcgag gtcacgagca ggtacaggtg gcccttgccc
      661 cgggtctgcg ccccgacgcg cggctcgaca tggagcgcgg tgtcaggcga atcgggggcg
      721 cgctcggggt cggggacgac accgagcttc atctggatcc ggtgggccac tgcggtcctc
      781 tgccaggttg ctggggcggg cggggattat cgcacggggt tcctgcgggt ctcggagacg
      841 tttaccggcg tcgcaccgtg acgcgcacct gacgcacctg cgctccgcct ggtgcccggc
      901 tgaacgcggc agcgagctcg gagccgtgga ggacgagctc ctggccgacg atcggggcgt
      961 cggcctcgac cacgagcgtc tcgccatcga ccttgagcgc ccggcaggcg ccgtgcgccg
     1021 ccggggtcag ctcggccacg atcgcgtcga acgtcgccag gaatcgggcg cggcggagct
     1081 cgtcctggag gccgagatgg gccgcggcct ggggcatcag gtcaccgatc ctcgacatcg
     1141 gccggcgccg cttgcgctgg acctccggat cgtcgctcac gacgcggacg acagcagccg
     1201 cgccccgtcg aggtcggccc gaacctccca cggcgtcgcg atcgcgcgca ggccggggtc
     1261 gaggtcgtcg agggtcgtcg tggtcacgaa cgcctgtggc agctcggcga tcctgcggac
     1321 gaggtggccc cgccgggcgg ggtcgagctc cgagaacacg tcgtcgagga ggagcaacgg
     1381 cggccggctg tcgtggctgg tcacgaggtc gagctcggcg agcttgaggg cgaggatcgc
     1441 cgtgcgctgc tggccgcgcg acgcgaaggc cgagagatcc cgcccggcca tcacgaacgc
     1501 gacgtcgtcc cggtgcggcc cgaccagggt cgtgccgttc cacatctcct tctccgcggt
     1561 ctcggcgagc cggcgcttga gggcgtcgcg aggggtctcg tccggcccgg ccggggcatt
     1621 ggtcacgtac tcggccccga gcgcgccggc cgctgcttcc tccggcgcga tctcggcgtg
     1681 cgccgccgcc agcggaccgg ccaggcgcgc aaggagctcg tgccgcgccg cgacaacggc
     1741 cccgccggca tcgaggaatg gctggtccca gtagcgcagc tcgtcccggg acgcggtctc
     1801 gtcacggatc gcgcgcaaca ggccgttgcg ctgctggagc gcccgggcgt aagtcgcgag
     1861 ctccgcggcg tagccgggca cgagggtcgt tgcgagctgg tccaacgtgg agcggcgcag
     1921 ggacggcgag ccggccacga ggagcatgtc ctccggcgcg aacagcacga cccggagtcg
     1981 ctcggcgagg accgccgccc ggcgtggcac gccgttgacc cggatccgct tgcgcgcgcc
     2041 gccggccgcc gccgcggagc cggggcggac cagggcaacc tcgatctcgt cgccgctcga
     2101 gcggccctcg atgcgggcca ggtccgcgcc ccaccgcacg agctccgcgt ccgtggtcgt
     2161 tcggtgcgac gagccgcgag caaggacgac catcgcctcg agcaggctgg tcttgccggc
     2221 cgcgttcggg ccccacacga gctgggggcc gccaccgaac tcggcctcga gcgtggcgta
     2281 cgcgcgcagg tccgcgagcc ggagtcgatc gatcgtcgcg accggggccg acgtggaccc
     2341 cgcggcgcca ggcccggggc ccatcgccgc tacgaagtgg tccggacggg catgaccacg
     2401 tggacgtact gatcgtcgcc gacgggcttg aagacgcccg gcgccagcgg gccctggagc
     2461 tcgagcgcga actgctcggc cgagaccttc tcgagcacgt cggcgaggta cttcgcgttg
     2521 aacgcgatcg acgtgccgtc gccctcgacc ttcgcctcga cctggcccac gtgatcgccg
     2581 atctcggcgt tcgcgctgac cgtgatcccc gattcgccgt tggcggagat cagcagcttc
     2641 acgatgttgg ccgattcatg ggcgatcagc gccgccggcc ggacggcgcg caagagctcc
     2701 tcgcgatcga ggatggcgcg cgtcgtgtgg gccttcggca ggaccgcctg gtagttcggg
     2761 aactggccgt cgatcagccg gctcacgagg tcgatgccct cgaggtggaa caggatctgg
     2821 ttgcggctct gggcaagcac gatctcgacc gggtcgtccg agtcggccag gacgcgcatg
     2881 agctcgatga gcgctcgcgc cggcacgacg acgctcgtgt cgcccaccgg atcgaggacc
     2941 gggatcgtct tcaccgcgat ccgatagttg tcggccgcgg cgagggtcac cgtctcgcct
     3001 tcgaagcgcc acaggacgcc ggtgaggatg ggtcgggcct catcggtcgc cgcggcgaag
     3061 gcggtctcct cgagggcccg tttcaggacg ttctgggcga tgcgggtggt cggtcgctcg
     3121 cccgtcggct ggatcgatgg gaattcgtcg gcatcgatgc ccttgacgtg cgactcgaac
     3181 cggccggact tcaggtggag cgtctcgccg gcgccgctct cgagctcgat ccggtcgccg
     3241 cccgagagcg agctcaccag gtccgacagg aggcgcgcgg ggacggtcgt cgccccgtcg
     3301 gtctcgacct tgccgggcac ccagtacacg agcccgatct cgaggttggt cgtggtgagg
     3361 cggaggccgc cgtcctcggt cttgaggagg acgttcgcca gcacgggcag cgtgctcctg
     3421 ttggagaccg ctcgactcac gacgccaagg ccgcgagcca ggttctcctg catgaccgag
     3481 agcttcacgt caggtccact ccatgggcgg gatcgaccag ggttcacacc aaggcggcgt
     3541 cagcacaggg gtttcccggc tcccgctcag ccgcgtcatt cggtgtgtgt atcttccttc
     3601 attcttcaat gctcgtcatc atcatcatca tggtgtggac ggcgtggacc gtcgtgctca
     3661 cccccgttcc cgaagccggt ggacggccga cgccaggcgg tggacccgac ctgattcgtc
     3721 ggggacggcc tcccggccga acggtgggca actcggaccc tcgcggtcgg cgcctccgaa
     3781 gttcgggccg cccgtccacc ggagccgagc gggttgtcca acattgcatc cacgttgcga
     3841 tcggccttcg tgaccttgcg acctcaccgg atctccacac accggggccg ttccgtggaa
     3901 aagtgccccg agcgatcagt cggcgtagat cagctcgcgg accgccgcga tttcgcgccg
     3961 gagctcgtcg ttgccgcctg actcgcgggt gatcttgtcg cacgcgtgga gcacggtcga
     4021 gtggtcgcgg ttgccgagct cggcgccgat gcgcaggagc gagacgtccg tctcctcgcg
     4081 catcaggaac atcgcgatct ggcggggcac gacgatcgcc ttgtcccgct tctggccctt
     4141 gag
//
LOCUS       DATBSW010000318         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_559283, whole genome shotgun sequence.
ACCESSION   DATBSW010000318 DATBSW010000000
VERSION     DATBSW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559283"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VIF63_08695"
     CDS             complement(<1..928)
                     /locus_tag="VIF63_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY8239499.1"
                     /translation="MLNAAAPSVVTFGEVMLRLAPVDGTRLASARVLDATFGGGEANV
                     AASLAGFGVPARFVTRVPANDLGEGAIRFLRGFGVDTSGITTEAGRLGVYYLEPGAAQ
                     RASRVVYDRADSCIARAGADAYAWPSLLDGARWFHTTGITPALSGPAAEATLAAVRAA
                     RAAGATVSVDLNYRAKLWSWGAAAGDVMAGIVAEADVLIGNEEDADKVFGIRAPGSAV
                     AEGRVDPAGYASVAAQLAERFPKLRTIAFTQRGSISASENTWSGVIWTADAFHVAKSY
                     RIAPIVDRVGAGDAFAAGLIYALLDERPLEAAL"
     gene            1137..>2507
                     /locus_tag="VIF63_08700"
     CDS             1137..>2507
                     /locus_tag="VIF63_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239500.1"
                     /translation="MLIDSAINLEDLRKPLSAFWPISGAKIANLFASWDPAKGSPVFT
                     VRGRYTSQGWTEWTQGFQFGWAILQYDATGDDRFLELGRRATVEHMAPHVSNVGVHDH
                     GFNNVSTYGNLWRLMREGRIPRDPWELAFYELALKASGAVQAARWRPTHDGPGYIYSF
                     NGPQSLFADTIRSVRSLFLAHQLGHALMGENDQPISLLRRGIEHAQTTARWNVFYGEG
                     RDSYDEWGRVAHESVFNVNDGRYRTPATQQGYSPFSTWTRGLAWVTLGYPEHLEYMDT
                     VRDDELDAFGGRGVVTDLLLRAARASCDFYIANTAADGVPYWDTGAPGLAQLPGWRDR
                     PSDPFNHHEPVDGSSAAIAAQGLLRLGGWLTARGDADGARYTAAGITALRTVLAQPYL
                     STDTDHQGLLLHSVYHRPQGWDFAPDADGVPRGEAVMWGDYHLVEAALLAQRMLDGGP
                     HLAFFGP"
BASE COUNT          382 a          916 c          858 g          351 t
ORIGIN      
        1 cgagggccgc ctcgagtggc cgctcgtcaa gcagcgcgta gatcagcccg gcggcgaagg
       61 cgtcgccggc gccgacgcga tcgacgatcg gggcgatgcg atacgacttc gcgacgtgga
      121 aggcgtccgc cgtccagatc acgccggacc aggtgttctc tgacgccgag atcgacccgc
      181 gctgggtgaa cgcgatggtc cgaagcttgg gaaaccgctc ggcgagctgc gccgcaacgg
      241 acgcgtaccc ggcgggatcg acgcgaccct cggccacggc gcttccaggc gctcggatgc
      301 cgaacacctt gtcggcgtcc tcctcgttgc cgatgaggac atcggcctcg gccacgatgc
      361 cggccatgac atcgccggcg gcagcacccc agctccagag cttcgcgcgg tagttgaggt
      421 cgaccgagac ggtggcaccg gccgcccgag cggcccgcac cgccgccagg gttgcctcgg
      481 cggccggccc agacagcgcc ggcgtgatgc cggtggtgtg gaaccaacga gcaccatcga
      541 ggagcgacgg ccacgcgtag gcgtcggcgc cggcccgtgc gatgcaggag tcggcccggt
      601 cgtagacgac ccggctggca cgctgcgcgg caccgggctc caggtagtag accccgagcc
      661 ggccagcttc ggtcgtgatg cccgatgtgt cgacgccgaa gccacggagg aaccggatgg
      721 cgccctcgcc caggtcgttc gccggcacgc gggtcacgaa ccgggccggg acgccgaagc
      781 ccgcgagcga ggcagcgaca ttggcctcgc cgccgccgaa ggtcgcgtcc aggactcggg
      841 cgctcgcgag ccgcgtgccg tccacaggcg cgaggcggag catgacctcg ccgaaggtga
      901 cgacgctcgg ggctgccgcg ttcaacacgc agtcattgtc ctcgttcctg cagggacggc
      961 cggggcgccc acggtacgcg ggcggtcgga ccgggcaaac ctatgggcgt cgccagatgg
     1021 tggacgaaag tgaagccgcg ccgcgggccg atgtagctcg ccgaacagtc tgaccgccca
     1081 actcgggccg ctaccatcca tcgcggctcg gtccgcactg cgcaacagga gatcccgtgc
     1141 tcatcgactc ggccatcaac ctcgaggatc tgcggaagcc gctctccgcc ttctggccga
     1201 tctcgggcgc gaagatcgcg aacctgttcg cgtcgtggga cccggcgaag ggctcgccgg
     1261 tgttcacggt ccggggccga tacacgagcc agggctggac cgaatggacg cagggcttcc
     1321 agttcggctg ggcgatcctg cagtacgacg ccacgggcga cgaccgtttc ctggagctgg
     1381 ggcggcgggc gacggtcgag cacatggcgc cacatgtctc caacgtcggc gtccacgacc
     1441 acgggttcaa caacgtctcg acgtacggca acctctggcg cctgatgcgc gagggccgca
     1501 tccctcggga cccctgggag ctcgcgttct acgagctcgc gctcaaggca tccggcgcgg
     1561 tccaggcggc gcgctggcgc ccgacccacg acgggccggg gtacatctat tcgttcaatg
     1621 gcccgcagtc gctctttgcg gacaccatcc ggtccgtccg ctcccttttc ctcgcccacc
     1681 agctcggcca cgccctgatg ggcgagaacg accagccgat ctcgctgctg cgccgaggca
     1741 tcgagcatgc ccagacgacc gcccgctgga acgtcttcta cggcgagggg cgcgattcct
     1801 acgacgagtg gggccgcgtc gcccacgaga gcgtcttcaa cgtcaacgat ggccggtatc
     1861 ggactcccgc gacgcaacag ggctactccc cgttctccac gtggacccgt ggcctcgcct
     1921 gggtcacgct gggctatccg gagcatctcg agtacatgga cacggtccgg gacgacgagc
     1981 tcgacgcgtt cggcggccgc ggcgtggtca ccgacctcct gctccgggcg gcccgggcat
     2041 cgtgcgactt ctatatcgcc aacacggccg cggacggcgt cccgtactgg gacacgggcg
     2101 cgcccggcct cgcccagctg ccgggctggc gcgaccgacc gtccgacccg ttcaaccacc
     2161 acgaaccggt cgacggctca tcggcggcga tcgcggcgca gggcctcctt cgactcggcg
     2221 gctggctgac cgctcgcggc gacgcggacg gtgcgcgcta caccgcggcc ggcatcacgg
     2281 cgctccgaac cgtcctcgcg cagccctacc tctccaccga caccgaccat cagggcctgc
     2341 tcctccacag cgtctaccac cggccgcagg gctgggactt tgcgccggac gctgacggcg
     2401 ttccccgcgg cgaggccgtc atgtggggcg actaccacct cgtcgaggcg gccctgctag
     2461 cccagcgaat gctcgacggc ggtccccacc tggcgttctt cggtccg
//
LOCUS       DATBSW010000319         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1229200, whole genome shotgun sequence.
ACCESSION   DATBSW010000319 DATBSW010000000
VERSION     DATBSW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229200"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3481)
                     /gene="mfd"
                     /locus_tag="VIF63_08705"
     CDS             complement(<1..3481)
                     /gene="mfd"
                     /locus_tag="VIF63_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY8239501.1"
                     /translation="MSSSPPMRGKRGWERRLAEHSSKVDAPTTEQPAFDSAEAEIEAH
                     ATATARLAEAAVAPTRTSAAQHGSGAAGSKQGPGRRLPNLAGLPRLLGDSGAFASLRE
                     RLGPTSAPASGAGRHAGVTSVPHGAKSYLTAALALVAGERICWIARDSEIGDRVVEEL
                     AAWVGDPAAVVVLEPRSALAYERSELVADETAARVAALSAWRSGTARILVASVQALIQ
                     HTIAPDDLPETPLELRVGTRIAQSALLRELLELGYAPVLEVAGRGEFARRGGIVDVFP
                     PSAELPVRIESFGDEIDSLRAFDPTDQRTTGKVDRVALLPASEFLVPEGGPDGLRARL
                     GPLVKRLPERLAADLERLVGAADADRPAAVHGESRAAAVGDAAEVWAPLLAPSTALDH
                     LAPETLFVIDEPGDVGEAADFLWRQADERRADLLGAGELPKDWPPTLLPPRDWKRRLL
                     GSRTLELTWESEANGAIAGGGKSSGDLFGWREPQLPPGRGQRIAEAVERWRAADGASP
                     RIVIASDQAPRLAELLEESGIAAGITNAIPEPPPAGALALIDRSLNGGFIGGPDRLVF
                     VTDRELFGNVRVRRPKAMRRVVPRDILERLTPGDLVVHIDHGVARYQQMLRRSSGTGD
                     ERDFLELAFQGQDRIFVPVEQIGRISRYSGGENPQLSKLGGTEWLRTKQRVRKAVADL
                     AEELLGLYAARASAPGHAYPDDTPWQGEMEAAFPYEETVDQLRAVVEVKHDMEAIQPM
                     DRLVVGDVGYGKTEVAIRAAFKATQDGKQVAVLVPTTVLAAQHYATFGARFGPFPVKV
                     ALLSRFVPKAEQDKAVAGLADGSVDLVIGTHRLLSRDVRFKDLGLVVVDEEQRFGVAA
                     KERLKQLKREVDVLTLSATPIPRTLNLALAGVRDMSVIETPPEDRLPIQTRVAEASAG
                     LVRDAILRELDRGGQVFYVHNRVETIEAQAEQLRKLLPDVRLVVGHGQMGEGQLESVM
                     LKFASGEADVLVCTTIIESGLDIPNANTIVIDRADTLGLAQLYQLRGRVGRSSRRAYA
                     YLLYRRRERMSEEARKRLQAIFNASELGAGFQIALSDLEIRGAGNILGGEQSGHMAAV
                     GFDLYSRLLAEAVEERKAERESRAPVLVAPQAVIDLPLEAHLPDDYVPEEAQKLELYR
                     RLSRARTPGDVAAF"
     gene            complement(3478..>3793)
                     /locus_tag="VIF63_08710"
     CDS             complement(3478..>3793)
                     /locus_tag="VIF63_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239502.1"
                     /translation="IGAPNDRFIDHVLTKFEPDERQRLDELLDAAADAVEGWARDGTN
                     RAATRFNAFELRPADTELAAPAGEIGGPPGPDGIRRTKTGWRKIRAKLTGSDSPDDEG
                     GR"
BASE COUNT          523 a         1386 c         1351 g          533 t
ORIGIN      
        1 ggaacgcggc gacgtcgccc ggcgtgcggg cacgcgacag acggcggtac agctcgagct
       61 tctgggcctc ttcggggacg tagtcgtcgg gcaggtgggc ctcgagcggc aggtcgatga
      121 ccgcctgcgg cgcaacgagc acgggagcgc gactctcgcg ttccgccttg cgctcctcga
      181 ccgcctccgc aagcagccgc gaatacaggt cgaaaccgac cgcggccatg tggcccgact
      241 gctccccacc gaggatgttg ccggcgccgc ggatctcgag gtcggacagc gcgatctgga
      301 acccggcgcc gagctccgag gcgttgaaga tcgcctggag ccgcttgcgc gcctcctcgc
      361 tcatccgctc gcgccggcga tagagcaggt acgcatacgc gcgccgcgac gaccggccca
      421 ctcggccccg gagctggtac agctgggcga gcccgagcgt gtccgcgcga tcgatcacga
      481 tcgtgttggc attcgggatg tccaggccgg actcgatgat cgtcgtgcac acgagaacgt
      541 cggcttcgcc cgacgcgaac ttgagcatca cgctctcgag ctggccctcg cccatctggc
      601 cgtgcccgac gacgaggcga acgtcgggca ggagcttgcg cagctgctcg gcctgggcct
      661 cgatcgtctc gacccggttg tggacgtaga agacttgccc gccccgatcg agctcgcgga
      721 ggatcgcgtc gcgcacgagg ccggccgacg cctcggcaac gcgggtctgg atcggcagcc
      781 ggtcctccgg cggggtctcg atgacgctca tgtcgcgcac cccggcgagg gccaggttca
      841 atgttcgcgg gatcggggtt gccgacaggg tcaggacgtc gacctcgcgc ttgagctgct
      901 tgagccgctc cttggccgcg accccgaacc gctgctcctc gtcgacgacg acgaggccca
      961 ggtccttgaa ccgcacgtca cgagacagca gccgatgcgt gccgatgacg aggtcgaccg
     1021 agccgtcggc gagccccgcc acggccttgt cctgctcggc cttggggacg aaccgcgaca
     1081 gcagcgcgac cttgaccggg aacggcccga accgcgcacc gaacgtcgcg tagtgctgtg
     1141 ccgccaggac cgttgtcggc acgagcaccg cgacctgctt cccgtcctgg gtggccttga
     1201 acgcggcccg aatcgcgacc tcggtcttgc cgtagccgac gtcgcccacg accagccgat
     1261 ccatcggctg gatcgcctcc atgtcgtgct tgacctcgac gacggcccgg agctggtcga
     1321 ccgtctcctc gtacgggaac gcagcttcca tctcaccctg ccagggcgtg tcgtccgggt
     1381 acgcgtggcc cggggccgag gcgcgcgccg catacagccc gagcagctcc tcggcgagat
     1441 ccgcgacggc cttgcggacc cgctgcttcg tgcgcagcca ctccgtgcca ccgagcttgg
     1501 acagctgcgg gttctcacca ccggagtagc gcgagatccg cccgatctgc tcgaccggga
     1561 cgaagatccg gtcctggccc tggaacgcga gctcgaggaa gtcgcgctcg tcgccggtgc
     1621 cggagctccg gcggagcatc tgctggtagc gagcgacacc gtgatcgatg tggacgacga
     1681 ggtcgccggg cgtgagccgc tcgaggatgt cgcgcggcac gacccggcgc atcgccttcg
     1741 gacggcggac gcggacgttg ccgaacagct cgcgatcggt gacgaacacg agccggtcgg
     1801 gaccgccgat gaagccgccg ttgaggctcc gatcgatgag ggcgagcgca ccggccggcg
     1861 gcggctcggg gatcgcgttc gtgatcccgg cggcgatgcc cgactcctcg aggagctccg
     1921 cgagccgcgg cgcctggtcc gacgcgatga cgatgcgcgg cgaggcgccg tcggctgcgc
     1981 gccagcgttc cactgcctcc gcgatgcgtt gcccgcgtcc cggcgggagc tgcggctcac
     2041 gccatccgaa caggtcgccc gacgacttcc cgccaccggc aatcgcgcca ttcgcctccg
     2101 attcccaggt cagctcgagc gtccgcgaac cgagcaggcg gcgcttccag tcacgcggcg
     2161 ggaggagcgt cggcggccag tccttgggca gctcgccggc gccgagcaga tcggcacggc
     2221 gctcgtcggc ctggcgccac aggaagtcgg ccgcctcacc cacgtcgccc ggctcgtcga
     2281 tgacgaagag cgtctcgggc gcgaggtggt ccagcgcggt cgagggggcg aggagcggtg
     2341 cccatacctc cgcggcgtcg ccgacggccg cggcgcgaga ttcgccatgg accgccgcgg
     2401 gccgatccgc gtctgcggca ccgacgagcc gctcgaggtc cgcggccagt cgctccggca
     2461 gacgcttcac gagcggtccg agccgggccc gcaggccgtc aggcccaccc tcgggcacga
     2521 ggaactcgga agccgggagg agcgccacgc ggtcgacctt gccggtcgtg cgctggtcgg
     2581 tcgggtcgaa cgcccgcagc gaatcgatct cgtcgccgaa cgactcgatc cggaccggca
     2641 gctcggccga cggcggaaac acatcgacga tgccgccgcg acgcgcgaac tcgccccggc
     2701 cggcgacctc gagcaccggc gcatagccga gctcgaggag ctcgcggagg agggcgctct
     2761 gcgcgatccg cgtcccgacc cgaagctcga ggggcgtctc gggcaggtcg tcgggcgcga
     2821 tcgtgtgctg gatcagggcc tggacgctgg cgacgaggat tcgcgccgtg ccgctgcgcc
     2881 aggcggaaag ggctgccacg cgagccgccg tctcgtccgc gacgagctcg ctgcgctcgt
     2941 acgcgagcgc gctccggggc tcgagcacga cgaccgccgc gggatcgccg acccacgccg
     3001 cgagctcctc tacgaccctg tcgccgatct cggagtcgcg tgcgatccag cagatccgct
     3061 cgccggcgac gagcgccagc gcggcggtga ggtagctctt ggcgccgtgc ggcaccgacg
     3121 tcacgcccgc gtgccggccc gcgccggacg ccggcgccga cgtcggcccg agccgctcgc
     3181 gcagggacgc gaacgctccg ctgtcgccca ggagccgcgg caatccggcc aggttcggga
     3241 gacgtcggcc aggaccttgc ttcgagcccg cggcgccgct gccgtgctgc gccgccgacg
     3301 tgcgagttgg ggcgaccgcg gcctcggcca gccttgccgt ggcggttgca tgggcctcga
     3361 tctcggcctc ggcggagtcg aacgcgggct gctcggtcgt gggcgcatcc accttcgacg
     3421 agtgctcggc aagacgccgc tcccagccac gcttgccgcg catcggtggg ctgctgctca
     3481 tcgaccgccc tcgtcgtctg gtgagtcgct gcccgtgagc ttggcgcgga tctttcgcca
     3541 gccggtcttc gtgcgccgaa tgccatccgg acccggcggc ccgccaatct cgcccgccgg
     3601 tgcggccagc tccgtgtccg cgggccggag ctcgaacgca ttgaaccgag tcgccgccct
     3661 gttggtcccg tcgcgcgccc agccctcgac cgcgtcggcg gcagcgtcga gcagctcgtc
     3721 gagccgctgg cgctcgtcgg gctcgaactt cgtcaggacg tggtcgatga agcggtcgtt
     3781 cggcgccccg atg
//
LOCUS       DATBSW010000320         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_630241, whole genome shotgun sequence.
ACCESSION   DATBSW010000320 DATBSW010000000
VERSION     DATBSW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630241"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VIF63_08715"
     CDS             <1..1319
                     /locus_tag="VIF63_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8239503.1"
                     /translation="RVDHALERLQGRSPNRVAVLFIDLDHFKSLNDRFGHAKGDDALR
                     VIGDRIKTIVRPGDTAGRLGGDEFAVLLEDVESTDVVSAVCERLLEGLALPIELGVAS
                     PIVGGSIGYALSGPEASTSEALLRNADVAMYAAKAGGRGQIVAFRPDLLETASARSDL
                     ANLLRGAESRNELQLAFQPVVNLDDATPVGVEALVRWQPPGLPLHLPAEFLELAEETG
                     EILPIGRWVLAEGCRRVRGWQTRYDLPDLRLYVNLSARQFRDPGLVPMIVAALARSGL
                     DPANLTLEITEGTLQTPGFETVQRIGELRALGLRLAIDDFGTGYSSLGYLHAFQIDEL
                     KIDRSFVPGSDGVGDAHVLSQAIVELGRALSLDMIAEGIETTDQADWFRTLGCRLGQG
                     YLYARPMPAAEVDRYLRREIQPRSLSAMRHAGTGITDVLGERKAAG"
     gene            complement(1332..2303)
                     /locus_tag="VIF63_08720"
     CDS             complement(1332..2303)
                     /locus_tag="VIF63_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY8239504.1"
                     /translation="MEATSAIRIQSWRAGKVTASSDVDGIGALVGTKGGQTWIDLTDP
                     SPELVQAVANQLGLHPLVAEDIIESNERAKVQLIDDVIHVVSFVLERTREVEAHEVDF
                     VLGRGFVLSVHHGGWDPTTAHQARLGIGALLAKGADYVLWAMIDAIVDGYFPVFDRLG
                     DEIDQVQDDAIERPVSATLERLFRLKRELIRIRHVVAPSREVFAQLTSREFGQISEPN
                     VFYFRDVYDHLIRLNDELDSFRELTAGSLEIYLSTINNNLSTIMKRLTGVTVILAGIG
                     AIGGLFGMSEAASALNGSEGSGFYLILLGTFVAAILGVWLLRRINWL"
     gene            2362..2937
                     /locus_tag="VIF63_08725"
     CDS             2362..2937
                     /locus_tag="VIF63_08725"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY8239505.1"
                     /translation="MSSGRLEAFSDAVIAIVMTIMVLELVPPHGSTLEDLFLLWPVII
                     AYVLSFAIIAIYWNNHHHLLQAAETVSGRVLWANMLLLFWLSLIPFGTAWIGETQFAN
                     IPMAVYGVILLACAFSYGFLVVALIRSPGQTETLAKALANDRKGRISPYIYAAAIPIA
                     FFAPYASFALYVFVAAMWIVPDPRIERRIAG"
     gene            complement(3214..3621)
                     /locus_tag="VIF63_08730"
     CDS             complement(3214..3621)
                     /locus_tag="VIF63_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8239506.1"
                     /translation="MRTTITGLVLAAFVAAFVAACAGSSTGALGFASAPADLDPNSPK
                     VAARDIDFDRTDVAVPAGRAFILVFENQESASHNVSIYRDAAFKDRIFEGVVFGGPAT
                     RWYPVPALTPGTYFFQCDIHPVPPMQGTITAGL"
     gene            complement(3636..4025)
                     /locus_tag="VIF63_08735"
     CDS             complement(3636..4025)
                     /locus_tag="VIF63_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239507.1"
                     /translation="MNALRPTSPAGLLVRAAIVGLTLATGWIHLGLGGVLFTLNGLGY
                     VVAAGAMIAPIGLAVRFRWFIRLGLIGYAIAAIVGWFLIGPRYDVAYIAKAIEVVLIG
                     LLLVEVRAYDGNPIRRIRQAAASLARA"
     gene            4290..4925
                     /locus_tag="VIF63_08740"
     CDS             4290..4925
                     /locus_tag="VIF63_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239508.1"
                     /translation="MDFELDETDEDRDFYRWYGAWAPFEPAWVAGLFADIPARWWIVG
                     GWAVDAFTGVARPHEDIDVGFLRADLPVVLEHLNADACVWTNVSGTIRPLRKPEDLLE
                     GSRQLWVRHNGDSPWFMDLAMTAHDGDTWISPRDERIRRPLDEATFLGDDGIRYLRPE
                     LVLFMKAKIARAKDDHDLGQVLPRLDPAAKAWLRESIELVHPGHRWLADLG"
     gene            complement(4957..>5337)
                     /locus_tag="VIF63_08745"
     CDS             complement(4957..>5337)
                     /locus_tag="VIF63_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HEY8239509.1"
                     /translation="IVLGLPLYGMRWRMSGPGLTTAVIGSGVTWVPNQHLDVLLGDGF
                     YPGRDPIEISEVFWTRDGVDWLVTTYDSPATLRPKMALARDNGLAGAGFWAMGYERGL
                     PGFVELMTDFRNGDVARTEAPTRQ"
BASE COUNT          823 a         1817 c         1846 g          851 t
ORIGIN      
        1 atcgcgtcga ccacgcgctc gagcgactgc aggggcgaag ccccaaccgc gtcgcggtgc
       61 tgttcatcga cctcgatcac ttcaagagcc tcaacgaccg cttcggccat gccaagggcg
      121 acgatgcgct gcgcgtgatc ggggaccgga tcaagacgat cgtccggccc ggtgataccg
      181 cggggcgcct cggcggcgac gagttcgcgg tgctgctcga ggacgtcgaa tcgaccgatg
      241 tcgtctcggc cgtgtgcgag cggctgctcg agggcctcgc cctcccgatc gagctcggtg
      301 tcgcgtcgcc gatcgtgggc gggagcatcg gctatgcgtt gagcggcccc gaggcgtcca
      361 cgagcgaggc cctgctgcgc aacgccgatg tcgcgatgta cgcagcgaag gccggcggcc
      421 gcggccagat cgtcgcgttc cggcccgacc tgctcgagac cgcgtcggcg cggagcgacc
      481 tcgccaacct gctgcgcggt gcggagtcgc gcaacgagct ccagctcgcg ttccagcccg
      541 tggtcaacct cgacgacgcg acgccggtcg gggtcgaggc gctggtgcgc tggcagcccc
      601 cgggcctccc gctccacctg cccgccgagt tcctcgagct cgccgaggag accggcgaga
      661 tcctgccgat cggacgctgg gtcctcgccg agggctgccg ccgggtccgc ggctggcaga
      721 cgcgctacga cctgccggac ctgcgcctgt acgtgaacct ctcggcgcgc cagttccgcg
      781 atccgggtct cgtcccgatg atcgtcgccg ccctcgcccg gtccggcctg gacccggcga
      841 acctcaccct cgagatcact gaggggaccc tgcagacccc gggcttcgag accgtccagc
      901 ggatcggcga gctgcgcgcc ctcgggctcc gtctcgcgat cgacgacttc ggcaccggct
      961 actcgtcgct cggctacctg cacgccttcc agatcgacga gctcaagatc gatcgctcgt
     1021 tcgtgcccgg cagcgacggc gtgggcgacg cgcacgtcct cagccaggca attgtcgagc
     1081 tcggccgcgc gttgagcctg gacatgatcg ccgagggcat cgagaccacc gaccaggccg
     1141 actggttccg cacgctcggc tgccgcctcg gccagggcta cctgtacgcc cggccgatgc
     1201 cggcggccga ggtcgaccgg tacctgcggc gcgagatcca gccgcgatcc ctcagcgcga
     1261 tgcgccacgc gggcacgggc atcaccgacg tgctcggaga gcgaaaggcc gcgggctgat
     1321 tcggttcgct cttagagcca gttgattcgg cgcaggagcc aaacaccgag gatcgcggcc
     1381 acgaatgtgc cgagcaggat caggtagaag ccggagccct cggagccgtt gagcgccgac
     1441 gcggcctcgc tcatcccgaa gaggccgccg atggcgccga tcccggcgag gatgacggtc
     1501 acgcccgtga ggcgcttcat gatcgtcgac aggttgttgt tgatcgtcga gaggtagatc
     1561 tcgagcgatc ctgccgtgag ctcccggaac gagtcgagct cgtcgttgag ccggatcagg
     1621 tggtcataca cgtcccggaa gtagaagacg ttcggctcgc tgatctggcc gaactcccgg
     1681 ctcgtgagct gggcgaagac ctcgcggctc ggggccacga cgtgtcgaat gcggatgagc
     1741 tcccgcttca accggaagag gcgctcgagc gtcgccgaga ccggccgttc gatcgcgtcg
     1801 tcctggacct ggtcgatctc gtcgccgagg cggtcgaaga ccgggaagta gccgtcgacg
     1861 atcgcgtcga tcatcgccca caggacgtag tcggcgccct tcgcgagcag cgctccgatc
     1921 cccaaccggg cctggtgggc cgtcgtcggg tcccagcccc cgtggtgaac cgagagcacg
     1981 aatccgcgac cgaggacgaa gtcgacctcg tgcgcctcga cctcgcgcgt acgctcgagg
     2041 acgaaggaca cgacgtgaat cacgtcgtcg atcagctgga ccttcgcccg ctcgttgctc
     2101 tcgatgatgt cctcggcgac cagcgggtgc aggccgagct ggttcgcgac cgcctggacc
     2161 agctccggcg aggggtcggt caggtcgatc cacgtctggc cgcccttcgt cccgaccagg
     2221 gcgccgatgc cgtccacatc gctcgacgcc gtcaccttgc cggcgcgcca gctttggatc
     2281 cggatcgccg acgtcgcttc cacgccgcga tcctacgcga tgccgtgcgc gccggagccg
     2341 tgagccctca tcatccgcgg catgagcagc ggccggctcg aggcgttcag cgatgcggtg
     2401 atcgcgatcg tgatgacgat catggtcctg gagctcgtgc caccccacgg ctcgacgctc
     2461 gaagacctgt tcctgctctg gccggtgatc atcgcctacg tcctgagctt cgcgatcatc
     2521 gcgatctact ggaacaacca ccaccatctg ctccaggcgg ccgagacggt cagcgggcgc
     2581 gtcctgtggg cgaacatgct gctgctgttc tggctgtcgc tcatcccgtt cgggacggcg
     2641 tggatcggcg agacacagtt cgcgaacatc ccgatggccg tgtacggcgt gatcctgctc
     2701 gcctgcgcgt tctcgtacgg cttcctcgtc gttgccctca tccgctcgcc gggccagacc
     2761 gagacgttgg cgaaggccct cgcgaacgat cgcaagggcc ggatctcgcc gtatatctat
     2821 gccgcggcga tcccgatcgc gttcttcgcg ccgtacgcct cgttcgcgct gtacgtgttc
     2881 gttgcggcga tgtggatcgt gcccgacccg cgcatcgagc gccgcatcgc aggctgacga
     2941 cagggatcaa acacggatgg ggccgcgagg aggccccgat gtccgcgtgg aggacgagcc
     3001 ggacacgatt caggtttccg agaccacatc gggcttcatt cgtgtccggg ttgtcctctg
     3061 gccggacatt gagcggcctc caggctgtcc ggggccacat ccggcctcat tcgtgtccgg
     3121 gttgtcctct ggccggacat cgaatgggcg ctcgggcaag ccccggcgac gtggatgccg
     3181 aggtgcgtgt tgtgaggccg tgaactcgtc tgctcaaagg cccgcagtga tcgttccctg
     3241 catcggcggg accggatgga tgtcgcattg gaagaagtac gtccccggcg tgagcgccgg
     3301 cacgggatac cagcgcgtgg ccgggccccc gaacacgacg ccttcgaaga tgcggtcctt
     3361 gaacgccgcg tcgcgataga tcgagacgtt gtgggacgcg ctctcctggt tctcgaagac
     3421 gaggatgaac gcccggcccg cggggaccgc gacgtccgtc cggtcgaaat cgatgtccct
     3481 ggcggcgacc ttcgggctgt tcggatcgag gtcggccggc gccgaggcga agcccagggc
     3541 gccggtgctc gagccggcgc acgccgcaac gaacgcagcg acgaacgcgg cgagcaccag
     3601 gccggtgatg gtggtgcgca tcgatcgatc ctccgtcagg ctcgggcgag cgaggccgcg
     3661 gcctgtcgga tgcgccggat cggattgccg tcgtaggccc ggacctcgac caggagcagg
     3721 ccgatcagga cgacctcgat cgccttggcg atgtacgcga cgtcgtagcg cgggcctatc
     3781 aggaaccagc cgacgatggc cgcgatggca tagccgatca ggccgaggcg gatgaaccag
     3841 cggaagcgga ccgcgaggcc gatcggggcg atcatcgcgc ccgcggcgac cacgtagccg
     3901 agcccattga gcgtgaacag cacgccgccg agcccgagat ggatccagcc ggtcgcgagc
     3961 gtgagcccga cgatggcggc ccggacgagc agtccggcgg gggaggtggg acgcagcgcg
     4021 ttcatggtgg accactcctg ctccgcgggg ctccgggttc ggccgggcgg ccggccctgt
     4081 cccgcaaatt cctgcttgct acttcgctgt agcaagtaat acacgacttg tggctactgt
     4141 caagtagcac gtttctgccc ggccggtcgg tccgaccttc gcgtgcctgg cggctctgtc
     4201 gtgtgacggt ataccgtatg acgatatacc gatacgcgtt atatgcgaca ggcgccgcgc
     4261 gtgtcaagac cccggccata cttggggccg tggacttcga gctggacgag accgacgagg
     4321 accgcgactt ctatcgctgg tacggcgcgt gggcgccgtt cgagccggcc tgggtggcgg
     4381 ggctctttgc cgacatccct gcacgctggt ggatcgtggg cggctgggca gtcgatgcgt
     4441 tcacgggcgt cgcacggccg cacgaggaca tcgacgtcgg gttcctgcgg gcggacctgc
     4501 cggtcgtcct cgagcacctg aacgccgacg cctgcgtctg gacgaacgtg agcgggacga
     4561 tccgcccgct gcgcaagccc gaggacctcc tcgagggcag ccggcagctg tgggtgcgcc
     4621 acaacggcga cagcccgtgg ttcatggacc tcgcgatgac cgcgcacgac ggggatacct
     4681 ggatctcacc gcgggacgag cgcatccgtc gaccgctcga cgaggcgacg ttcctcggcg
     4741 acgacggcat ccgctacctg cgccccgagc tcgtgctgtt catgaaggcg aagatcgcgc
     4801 gggccaagga cgaccacgat ctcgggcagg tcctgccgcg gctcgatccg gcggcgaagg
     4861 cgtggctgcg tgagtcgatc gagctggtgc acccgggcca tcgctggctg gcggacctgg
     4921 gctagggccg gccggcgccc tgggtccggg ccgcgcctac tgtcgagtcg gcgcctcggt
     4981 gcgggcgacg tcgccgttgc ggaaatcggt catgagctcg acgaagccgg gcaacccgcg
     5041 ctcgtagccc atcgcccaga agccggcgcc ggccagtccg ttgtcgcgcg cgagcgccat
     5101 cttcggacga agcgtcgccg gtgagtcgta ggtcgtgacc agccagtcga cgccatcgcg
     5161 agtccagaac acctcggaga tctcgatcgg atcgcgaccg ggatagaacc cgtccccgag
     5221 cagcacgtcg agatgttggt tcgggaccca cgtgacgccg ctgccgatga ccgcggtggt
     5281 gagaccgggt ccggacattc gccagcgcat cccgtacagc ggcaggccga gcacgat
//
LOCUS       DATBSW010000321         9362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_665715, whole genome shotgun sequence.
ACCESSION   DATBSW010000321 DATBSW010000000
VERSION     DATBSW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665715"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VIF63_08750"
     CDS             <1..511
                     /locus_tag="VIF63_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239510.1"
                     /translation="ELRLAPQLATRGTATGALYGYAVNAAPGSSLALSSWSTFGPYTY
                     ALASPLSMTDPQGMLTGLGFGVITGIVLAPVLAAGIGLGAGIVVGLAIGVGAALLIGE
                     VVSNNSFLNRCSPGFFGAPDGDPFFGSAPAAPPVPPAPAPLPPDPGLDSGYEVQVSAP
                     DGGGAPIKG"
     gene            complement(532..2208)
                     /locus_tag="VIF63_08755"
     CDS             complement(532..2208)
                     /locus_tag="VIF63_08755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239511.1"
                     /translation="MGALTRPIRSLIGSLREAMSNEGIRRLEASWALGIAADTGLLVV
                     LIIVIFLRDGVVAAGVLGAARMVPAVLSGMLAGASVERFRGDRVLLVVGLTRAVAAGL
                     CALVIATDSSTLLLFGLAAVVAAAGALVRPTQATLMPAIARSPRELVAANMAWSTGEG
                     LGAMIGPFVAGLLVAAGHPEAGAAAAALAFLASALSVVGLRFEQARDATGGAGSAGGG
                     IRLLDGLRTWRRRPVVGWSMLGVFSQVMTRALLSPLIVVAAIELLLMGEAGVGLLNAA
                     LGLGGLVGAVFAISLTRTDRLIRTQALALSYWGAPIAVIGLLPSPALALAAMVVIGVA
                     NAVFDVALFTIMQRGTANEERAPVFSVFEGIVGLGTVAGSILAPVLLAAFGIRGALAV
                     SGAILPIVALVIYARIGRADKVSVIDEPTLQLLREVHVFAELPLTAFERLAGGLEPLS
                     FAEGDTIMREGEPGDRFVVIASGDVQVSAEGRPMQRLGRGAGLGEISLLRRSPRTATV
                     TALTPVTGYAIPGSCFVAAVSGPAAAAVTERIAAANILRGAAGDARGAIA"
     gene            2261..2674
                     /locus_tag="VIF63_08760"
     CDS             2261..2674
                     /locus_tag="VIF63_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8239512.1"
                     /translation="MIDKPAVLRGVPLFADLDEHSLQAVAVLAREEAHKATEILMLEG
                     EQGDEFFVIVDGTVRIERGGRAIRSMGAGGFLGEIALLEKRPRTATATCETDVSLLAM
                     HQHEFDRLLETMPAVHRRVRAAIDRRQRSGDSASL"
     gene            complement(2671..4398)
                     /locus_tag="VIF63_08765"
     CDS             complement(2671..4398)
                     /locus_tag="VIF63_08765"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8239513.1"
                     /translation="MTETNTAGPTTDAGAALRTLANAFWDGYLAMHPTWATVIGERRF
                     DDHLEDVSPNAIQGRLDWLDRTAGAALGIPNAELSPIERVTRQMLIDESRGEAASLRT
                     RLYEWTVDPLGGPTVGLLDLVDYQTITTPEDGRALVARWRDVDRYLDQLSANLREAAA
                     DGLVAVTNPVERVLDVLAHLDETPAEEWKLAAPALADHPDWSEQDRRRFRGEVFGAVR
                     DVVAPAFHRYRIVLEQHIRPSARPSNRPGLVHLPDGETAYRDCIRFHTTLDLEPEEIH
                     ATGLAEIARIDAEFVELGRRVLGTTDLASTLAALRNDRRLRFETTEEVFETAKRSLAR
                     ATAAVPEWFGRIPAAACVVVPVPSHSEVHQTIAYYSWPALDGSRPGRYYINLHAPETR
                     PRYEAEALAFHEAVPGHHLQIAVAQELPGLPAFQRMLGSTAFAEGWGLYTERLSDEMG
                     LYTSDMDRFGILSFDAWRAGRLVVDTGMHALGWTRQRAINFLKEHTALGENNIVNEVD
                     RYIVWPGQALAYKIGQLEILRLREVAKDRLGSAFDIKAFHDTVLGSGAVSLPVLGSLV
                     EDWLAAPRG"
     gene            complement(4416..5591)
                     /locus_tag="VIF63_08770"
     CDS             complement(4416..5591)
                     /locus_tag="VIF63_08770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEY8239514.1"
                     /translation="MNPLDVAAIVLLVLAVILGVRSGALPQVLGLVGAAIAALTGLAV
                     LPAFTPLIEDLPAAVRAIIVLSALLGLIGIGEALGAMGGRSMSQSLGNGFFGALDRAA
                     GALVGAAQAILILWLAGGVIASGPFPTLSQIAQKSTALRVVDGFLPPPTEIVLSLGNL
                     LDDSGLPNVFIGLEQLPAPDIDLPSDAIARAIGERAAPSVLRVIADGCGQRASGTSFV
                     IAPGYLVTNGHVVVGATNVFVQTSGDSFTATPVLVDLELDVALLYAPGVDAKPLLFTT
                     AEPTRGAQGAAVGYPGGGKETVEPATVAATYFATGRDVTGSARVTRRILELRAKVAPG
                     DSGGPFVLEDGTVGGVVFAESRVDPAVGYALAPKQVLDRVAPSLGRTTAADTGPCVP"
     gene            complement(5716..6333)
                     /locus_tag="VIF63_08775"
     CDS             complement(5716..6333)
                     /locus_tag="VIF63_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239515.1"
                     /translation="MLTTRACALLIAFVLSACAGAAAPTASPGAAATPKPTPTAVPGD
                     PGTGNGSGTDPGSGGGSGGGGTGGDPGTGGGIVIPIPGDPNQNPLFGDANYLTPAPNL
                     QNPRAMNVQLVRAIVVDDGTVTADLRWWSGVVPCNQLDHVDIVRDDAAHTIHLTVFEG
                     SGPGDVMCIELAELHATSVDLGDLAAGSWTISAEGDAPAITLEVK"
     gene            complement(6359..6985)
                     /gene="leuD"
                     /locus_tag="VIF63_08780"
     CDS             complement(6359..6985)
                     /gene="leuD"
                     /locus_tag="VIF63_08780"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY8239516.1"
                     /translation="MAEPYASFTSRVIPLPAENIDTDQIVPARYLKVTDKEGLGEALF
                     RDWRYEADGSLKTPEFVLDKPEMAGRRILLAGDNFGCGSSREHAPWALVAWGITAVIT
                     TSCADIFRGNALKNGLLPINVAPERLRQLFELVERDPSVELTVDLESEVVHLPGDEDL
                     PFDVDPFSKFMLLRGTDEMGYLLAKTDAIDAWEQAHPARIDTRPTAGT"
     gene            complement(6999..8450)
                     /gene="leuC"
                     /locus_tag="VIF63_08785"
     CDS             complement(6999..8450)
                     /gene="leuC"
                     /locus_tag="VIF63_08785"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY8239517.1"
                     /translation="MDAVRNAPRTLVDKIWDDHVISEGTNAPSVLAIDLHLVHEVTSP
                     QAFTGLRRRGLTVRHPERTVATADHSVPTTPRNLPILDAMAAAQVDQLTTNCREFGIP
                     LHGLGDPSQGIVHVIGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEMVLA
                     TQALLQRRPKTYEVRVDGRMAPGVSAKDIILNLLSRIGVAGGTGHVFEYRGSAIEALS
                     MDQRMTVCNMSIEGGARAGLIAPDDTTFEYLEGRAHAPKGTDWDAAVERWRSLPTDEG
                     AMFDKTISIDASALEPMITYGTNPGMGLPISANVPDPTAAEAGPGRRALEAALGYMDL
                     TPGQPILGQKVDVVFIGSCTNGRISDLRLAAAAMDGRKVADGVRMMVVPGSDEVKREA
                     EREGLHEIFRAAGADWREAGCSMCIAMNGDQLTPGQYAVSTSNRNFEGRQGKGGRTFL
                     ASPLTAAASAVAGVIADPRLLPGIERLAAVGGR"
     gene            complement(8506..>9362)
                     /locus_tag="VIF63_08790"
     CDS             complement(8506..>9362)
                     /locus_tag="VIF63_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY8239518.1"
                     /translation="GAVATQSFANVTYGPDGLDHLGAGLSASQAMDRLVGADPLRAQR
                     QAGIVDAHGGAATHTGSSCFAWAGGQIGPGFAAQGNILAGPAVVDGLVDTFLGGGRPF
                     PELLVACLAAADAAGGDRRGRESASLLVVRAGGGYGGGNDRWIDLRVDHHDDPIGELG
                     RHLELHHLYLDRPAVEELLPIDEALAGELVRLLKRVGAEPGGPLATLYEQMDPDAAAT
                     ASGDDGERPMTGTPRELPRGWDPSWHGALGDWMGVENLEERTAAPGWIDPRVLAVLRS
                     KAAAARRR"
BASE COUNT         1340 a         3331 c         3407 g         1284 t
ORIGIN      
        1 cgagctgcgg ctggcgccgc agctcgcgac ccgcggcacc gcgacgggcg cgctctatgg
       61 ttatgccgtc aacgcggcac cgggatcgag tctggcactc tcgtcgtgga gcaccttcgg
      121 tccatacacc tacgcgctcg catcgccgct ttcgatgacc gacccgcaag gaatgctcac
      181 gggccttggt tttggcgtga taacgggcat cgtgttggcc ccggtgctgg cagccggcat
      241 cgggctaggt gccggaatcg tcgtcggact cgcgatcggg gtgggtgcgg cgctcctcat
      301 cggcgaagtt gtttcgaaca actccttcct caaccggtgc tcacccgggt tcttcggcgc
      361 tcccgacggc gatccgttct tcggctcggc tcccgctgcg ccgccggtgc ccccagcacc
      421 ggcacctctg ccacctgacc ccgggctcga ttccggttat gaggtgcaag tctcggctcc
      481 ggatggaggt ggcgctccga tcaagggata gcgccctgac gagagccaac ctcaggcgat
      541 ggcgcctcgc gcgtccccag cggcgccgcg caggatgttt gccgcggcga tgcgctcggt
      601 gacggcggcc gcagccgggc ctgacacggc ggcgacgaag cacgagccgg ggatcgcgta
      661 gccggtgacg ggcgttagcg cggtgacggt ggccgttcgc ggcgagcgtc ggagcagcga
      721 gatctcaccg agccccgcgc cccggcccag gcgctgcatc ggccggccct cggcggagac
      781 ctggacgtcg cccgacgcga tgaccacgaa gcggtcgcct ggctcgccct cgcgcatgat
      841 cgtgtcgccc tctgcaaatg agagcggttc gaggccgccg gccagtcgct cgaaggcggt
      901 gagcggcagc tcggcgaaca cgtgtacctc acgcagaagc tggagcgtcg gctcgtcgat
      961 gacgctgacc ttgtcggccc tgccgatccg ggcgtagatg accagcgcga cgatcgggag
     1021 gatggcgccg gacaccgcga gcgcgccgcg gatcccgaac gccgcgagca ggaccggcgc
     1081 gaggatgctg ccggcgaccg tgcccagccc gacgatgccc tcgaacacgg agaagaccgg
     1141 cgcccgttcc tcgttggccg tgccgcgctg catgatcgtg aacagggcga cgtcgaagac
     1201 ggcgttcgcc acgccgatca cgaccatcgc cgccagcgcg agcgcgggcg agggcagcag
     1261 cccgatcacg gcaatcggcg caccccagta ggacagcgcg agggcctgcg tccggatcag
     1321 gcggtccgtg cgggtgaggc tgatcgcgaa caccgccccg accaggccgc cgaggccgag
     1381 cgccgcgttc agcaggccga cacccgcctc gcccatcaag agcagttcga tcgcagcgac
     1441 cacgatgagc gggctcagca gcgcgcgggt catgacctgg ctgaacacgc cgagcatcga
     1501 ccagccgacg accggccgcc ggcgccaggt ccgcagcccg tcgagcagcc ggattccgcc
     1561 gcctgccgaa ccggcgccac cagtggcgtc acgcgcctgc tcgaaccgca gtcccacgac
     1621 gctgagcgcc gaggcgagga acgccagcgc cgccgccgcc gcccccgcct ccgggtggcc
     1681 ggcggcgacc agcaggccgg cgacgaacgg cccgatcatc gcgccgaggc cctcgcccgt
     1741 gctccaggcc atgttggcgg ccacgagctc ccgcgggctg cgcgcgatcg cgggcatgag
     1801 cgtggcctgg gtcggccgga cgagcgcacc cgccgccgcg acaaccgccg cgagcccgaa
     1861 caggagcagg gtggaggagt cggtcgcgat cacgagagca cacaaccctg ccgcgaccgc
     1921 ccgcgtcagc ccgacgacga ggaggacccg atcgccacgg aatcgctcga cggacgctcc
     1981 cgcgagcatc ccggacagga ccgccggcac catccgcgcc gcgccgagga cgccggccgc
     2041 gacgaccccg tcgcgcagga agatcacgat gatcaggacg acgagcaggc ctgtgtccgc
     2101 agcgatgccg agcgcccagg acgcctcgag gcggcggatg ccctcgttcg acatcgcctc
     2161 gcgcagcgat ccgatgagcg accggatcgg acgcgtcaga gcccccatcg ggccaaggat
     2221 gacagcccgc gcagccgcgg gccgtcgcta tgctcacgcc gtgatcgaca agcccgccgt
     2281 cctgcggggc gtcccgctct ttgccgacct cgacgagcac agcctccagg ccgtggcggt
     2341 cctcgcccgc gaggaggcgc acaaggccac ggagatcctg atgctcgagg gcgagcaggg
     2401 cgacgagttc ttcgtgatcg tcgacggcac ggtccggatc gagcgcggcg ggcgggcgat
     2461 ccgatcgatg ggcgcgggcg gcttcctcgg tgagatcgcg ctcctggaga agcgaccccg
     2521 gacggccacc gcgacctgtg aaaccgacgt ctcgttgctg gcgatgcacc agcacgagtt
     2581 cgaccgcctg ctcgagacga tgccggcagt gcaccggcgt gtccgtgcgg cgatcgaccg
     2641 ccgccaacgg agcggcgact cggcgtcgct ctagccgcga ggcgccgcga gccagtcctc
     2701 cacgagcgag cccagcaccg gcaggctgac cgcgcccgac cccagcacgg tgtcgtggaa
     2761 ggccttgatg tcgaaggcgc tgccgagccg gtccttcgcc acctcgcgca gccgcaggat
     2821 ctcgagctgg ccgatcttgt acgcgagcgc ctggccgggc cagacgatgt accgatcgac
     2881 ctcgttcacg atgttgttct cgccgagggc ggtgtgctcc ttcaggaagt tgatcgcgcg
     2941 ctggcgggtc cagccgagcg cgtgcatccc ggtgtcgacg acgagccggc cggcgcgcca
     3001 tgcatcgaag gagaggatcc cgaaccggtc catgtcggac gtgtacaggc ccatttcgtc
     3061 ggacaggcgc tccgtgtaca ggccccagcc ctcggcgaag gcggtcgagc cgagcatgcg
     3121 ctggaacgcg ggcaggcccg gcagctcctg cgcgacggcg atctgcaggt gatggcccgg
     3181 cacggcctcg tggaacgcca gcgcctccgc ctcgtagcga ggtcgcgtct ccggcgcgtg
     3241 gaggttgatg tagtagcggc ccgggcgtga gccatcgagc gcgggccacg agtagtacgc
     3301 gatcgtctgg tggacctcgg agtggctcgg gaccggcacg acgacgcacg ccgccgccgg
     3361 gatccggccg aaccactccg gcacggcggc cgtggcacgg gcgagcgagc gcttcgccgt
     3421 ctcgaagacc tcctccgtgg tctcgaagcg caggcgccgg tcgttgcgca gcgcggcgag
     3481 cgtcgaggcg aggtccgtcg tgcccaggac gcggcggccc agctccacga actcggcatc
     3541 gatgcgcgcg atctcggcga ggccggtggc gtggatctcc tcgggctcga ggtcgagcgt
     3601 ggtgtggaac cggatgcaat cgcgatacgc cgtttcgccg tcgggcaggt gcacgaggcc
     3661 cggccggttc gacggccggg cgctgggccg gatgtgctgc tcgagcacga tccggtaccg
     3721 gtggaacgcc ggcgccacca cgtcccgaac cgcgccgaat acctcacccc ggaaccggcg
     3781 cctgtcctgc tcggaccagt ccggatggtc ggccagcgca ggcgccgcca gcttccactc
     3841 ctcggcgggc gtctcgtcga ggtgggccag cacgtccagc acgcgttcga ccgggttcgt
     3901 cacggcgacg aggccgtccg cggccgcctc gcgcaggttc gccgacagct ggtcgaggta
     3961 tcgatcgaca tcgcgccagc gcgcgacgag cgcgcggccg tcctcgggcg tcgtgatcgt
     4021 ctggtagtcg acgaggtcga gcaggccgac cgtcgggccg cccaggggat cgacggtcca
     4081 ctcgtacagc cttgtccgca gcgatgccgc ctcgccgcgc gactcgtcga tcagcatctg
     4141 gcgggtcacg cgctcgatgg gcgagagctc cgcgttcggg atcccgaggg cggcgccggc
     4201 cgtgcggtcg agccaatcga ggcggccctg gatcgcgttc ggcgacacgt cctcgaggtg
     4261 gtcgtcgaac cgtcgctcgc cgatgacggt cgcccacgtg gggtgcatcg ccaggtagcc
     4321 gtcccagaag gcgttggcaa gcgtccgcag ggcggcgccg gcgtcggtcg taggcccggc
     4381 ggtgttcgtc tcggtcatgg cccgatccta gcccgctagg ggacgcacgg ccccgtgtcg
     4441 gcggcagtcg tgcgcccgag cgacggcgcg acgcgatcga gcacctgctt gggcgcgagc
     4501 gcgtagccga ccgccggatc gacccgcgac tcggcgaaca cgacgccgcc gacggtgccg
     4561 tcctcgagca cgaacgggcc gccactgtcg cccggcgcga ccttggcccg aagctcgagg
     4621 atccggcgcg tgacccgagc ggaaccggtc acatcgcggc cggtcgcgaa atacgtcgcc
     4681 gccaccgtcg ccggctcgac ggtctccttg ccgccgcccg ggtagcccac ggcggcgccc
     4741 tgtgcgccgc gcgtcggctc ggcggtggtg aacaggagcg gcttcgcgtc cacgccgggc
     4801 gcgtagagga gggcgacatc gagctcgaga tcgacgagga ccggcgtggc ggtgaacgag
     4861 tcgcccgatg tctgcacgaa gacattcgtg gcgccgacaa ccacgtgccc gttcgtcacc
     4921 aggtagcccg gcgcgatcac aaaggacgtg ccgctcgccc gctggccaca gccgtcggcg
     4981 atcacgcgca gcaccgacgg cgccgctcgc tcgccgatgg cccgcgcaat cgcatccgac
     5041 gggaggtcga tgtccggggc cggcagctgc tcgagcccga tgaacacgtt cggcagcccg
     5101 ctgtcgtcga gcaggttgcc gaggctcagg acgatctcgg tcggcggcgg caggaagcca
     5161 tcgacgacgc gcagggcggt cgacttctgg gcaatctggc tgagcgtcgg gaacgggccc
     5221 gacgcgatga cgccgccggc cagccacagg atcaggattg cctgggccgc cccgaccaac
     5281 gcacctgcgg cgcgatccaa cgcgccgaag aagccgttgc ccagcgactg ggacatcgat
     5341 cggccgccca tggcgccgag cgcttcgccg atcccgatca gccccagcag cgcggacagg
     5401 acgatgatcg ctcggaccgc cgcgggcagg tcctcgatca gcggcgtgaa ggcgggcagg
     5461 accgcgagtc cggtgagtgc ggcgatcgcg gcgccgacga ggccgaggac ctggggcagc
     5521 gcgccggatc gcacgccgag gatgaccgcg agcaccagca ggacgatcgc ggccacatca
     5581 agggggttca ccgcgggatc gtgccacacg gcaccggggt gggtgtctca gacggtcgtc
     5641 ggtctcggcg ccgcctcgcc gcaggtggct tgcagttcct cgccgggctc gcgcggcagg
     5701 ccgcgcgctg tctggctact tgacctcgag cgtgatcgcg ggtgcgtcgc cctcggccga
     5761 gatcgtccac gagccggcgg caaggtcgcc caggtcgacg ctcgtggcgt ggagctccgc
     5821 gagctcgatg cacatcacgt cacccgggcc ggagccctcg aacacggtga ggtggatggt
     5881 gtgggccgca tcgtccctga cgatgtccac gtggtcgagc tggttgcacg gcacgacgcc
     5941 gctccaccag cgcaggtccg ccgtcacggt cccgtcgtcc acgacgatcg cccggacgag
     6001 ctggacgttc atcgctcgcg ggttctggag gttgggggcg ggcgtgaggt agttcgcgtc
     6061 gccgaagagc ggattctggt tcgggtcgcc cgggatcggg atgacgatcc cgccgccggt
     6121 gcccgggtcg ccgccggtgc cgccaccgcc ggagccgccg ccgctccctg ggtccgtacc
     6181 ggagccgttg ccggtgccgg gatcacccgg gaccgcggtg ggcgtcggct tcggcgtggc
     6241 cgccgccccc ggtgacgcgg tcggagccgc ggcgccggcg cacgcggaga gcacgaaggc
     6301 gatgagcagg gcgcacgcgc gagttgtcag catggccgcg agacgcccgc ttggggcgtc
     6361 aggttccggc ggtcgggcgg gtgtcgatgc gggccgggtg ggcctgctcc caggcgtcga
     6421 tggcgtcggt cttggcgagg aggtagccca tctcgtccgt tccgcgcagg agcatgaact
     6481 tcgagaacgg gtcgacgtcg aacggcaggt cctcgtcgcc gggcaggtgc acgacctcgg
     6541 attcgaggtc gaccgtcaat tcgacgctcg ggtcgcgttc gaccagctcg aacagctggc
     6601 gcagccgctc gggcgcgacg ttgatcggga gcagcccgtt cttgagcgcg ttgccgcgga
     6661 agatgtcggc gcagctcgtc gtgatcacgg cggtgatgcc ccacgccacg agcgcccacg
     6721 gcgcgtgctc gcgcgacgag ccgcagccga agttgtcgcc cgcgagcagg atccggcgtc
     6781 ccgccatctc gggcttgtcg aggacgaatt ccggggtctt gagcgagccg tcggcctcgt
     6841 agcgccagtc gcggaacagc gcctcgccga ggccctcctt gtcggtgacc ttgaggtacc
     6901 gcgcgggaac gatctgatcg gtgtcgatgt tctcggcggg cagcgggatg acgcggctgg
     6961 tgaagctggc gtacggctcg gccatcaggg cgttcccatc agcggccgcc cacggcagcg
     7021 aggcgttcga tgccgggcag gaggcgcggg tcggcgatca cgccggcgac cgcggaggcg
     7081 gccgccgtga gtggcgaggc aaggaacgtt cggccgccct tgccctggcg gccttcgaag
     7141 ttgcgattcg acgtgcttac cgcgtactgg ccgggcgtga gctggtcgcc gttcatcgcg
     7201 atgcacatcg agcagcccgc ctcgcgccaa tcggcgccgg cggcccggaa gatctcgtgc
     7261 aggccctcgc gctcggcctc gcgcttcacc tcgtccgagc ccggcacgac catcattcgg
     7321 acgccgtcgg cgaccttccg cccgtccatc gccgcggcgg cgaggcgcag gtcgctgatc
     7381 cgcccgttcg tgcagctccc gatgaacacg acatcgacct tctggccgag gatcggctgc
     7441 ccgggcgtga ggtccatata gccgagcgcc gcctcgagcg cgcgccggcc gggacctgcc
     7501 tcggcggccg tcgggtccgg cacgttcgcg gagatcggca ggcccatgcc ggggttcgtg
     7561 ccgtacgtga tcatcggctc gagggcggag gcgtcgatcg agatcgtctt gtcgaacatt
     7621 gcgccttcgt cggtcggaag cgagcgccac cgctccaccg cggcgtccca gtcggtgccc
     7681 ttcggggcgt gggcgcggcc ctcgaggtac tcgaacgtcg tgtcgtcggg cgcgatgagc
     7741 cccgcccgag ccccgccctc gatcgacatg ttgcagacgg tcatccgctg gtccatcgag
     7801 agcgcctcga tcgccgagcc ccggtactcg aacacgtggc cggtgccgcc ggcgaccccg
     7861 atccgcgaca gcaggttgag gatgatgtcc ttcgcactga cgccgggcgc catccgcccg
     7921 tcgacgcgga cctcgtacgt cttcgggcgc cgttggagca gcgcctgggt cgcgaggacc
     7981 atctcgacct cggacgtgcc gatgccgaac gcgagcgccc cgaacgcgcc gtgggtcgag
     8041 gtgtgcgagt cgccgcacac gatcgtcatg cccggctggg tcaacccgag ctgcggcccg
     8101 atgacgtgga cgatgccctg gctcgggtcg ccgaggccgt ggagcggaat gccgaactcg
     8161 cggcagttcg tcgtgagctg gtcgacctgg gcggcggcca tcgcatcgag gatcggcagg
     8221 ttgcgcgggg tcgtcgggac ggagtggtcg gccgtcgcca cggttcgctc cgggtgccga
     8281 acggtcagcc cgcggcggcg gaggccggtg aacgcctgcg ggctggtgac ctcgtggacg
     8341 aggtggagat cgatcgccag cacgctcggc gcgttggtgc cctccgagat gacgtggtca
     8401 tcccagatct tgtcgacgag cgtgcgcggc gcattccgga cggcatccat gtgcggcgca
     8461 ttctgccgga ccgcgccgaa tcggcgatca cccgatggtc gcgggtcagc ggcggcgggc
     8521 ggcggcggcc ttcgagcgca ggacggcgag cacgcgcgga tcgatccagc cgggcgccgc
     8581 ggtgcgctcc tccaggttct cgacgcccat ccagtcgccg agcgctccgt gccacgaagg
     8641 gtcccagccg cgcggcagct cgcgaggcgt tccggtcatc ggacgctcgc cgtcgtcgcc
     8701 gctcgccgtc gccgcggcgt cggggtccat ctgctcgtac agcgtcgcga gcggcccgcc
     8761 cggctccgcg cccacgcgct tgagcaggcg cacgagctcg ccggcgagcg cctcgtcaat
     8821 gggcaggagc tcttcgaccg caggccgatc gaggtacagg tggtggagct cgagatgtcg
     8881 cccgagctcg ccgatgggat cgtcatggtg gtcgacccgg aggtcgatcc agcggtcgtt
     8941 gccgccgccg tacccgccgc ccgcgcggac caccagcagc gacgccgatt cgcgcccacg
     9001 tcgatcgccg cccgccgcat ccgccgccgc gaggcaggcc acgaggagct ccggaaacgg
     9061 ccggccgccg ccgaggaacg tgtcgaccag gccgtcgacg accgccggcc cggcgaggat
     9121 gttgccctgg gccgcgaacc ctggcccgat ctgcccgccc gcccaggcga agcacgaaga
     9181 tccggtgtgg gtcgctgcgc cgccgtgggc atcgacgatc ccggcctgcc gctgcgcccg
     9241 cagcggatct gcaccgacga ggcgatccat cgcctgcgac gccgacagcc cggcgccgag
     9301 gtggtcgagg ccgtccggtc cgtaggtcac gttcgcgaac gactgcgtgg cgaccgcgcc
     9361 ga
//
LOCUS       DATBSW010000322         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1229520, whole genome shotgun sequence.
ACCESSION   DATBSW010000322 DATBSW010000000
VERSION     DATBSW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229520"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VIF63_08795"
     CDS             <1..1140
                     /locus_tag="VIF63_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239519.1"
                     /translation="TELTVANTTTVAAAPQATGGPIDGPTVVTGLDLEITSRIEVRAM
                     QAQPAQGTTSATGLIKLTRNADGVWSGTGNLASSTEVTSSVSCQTTNITGDGTYDWVV
                     RDVHAGPELGANLRVGMDSGPTNESPDTVSSDLCGTPSDSTMNTWENLFFDAYRPNYL
                     KNGGFLVDGWTILDPAAWTTGGAVAEAHWSGACTGPDLGLPGMSVKLLDCSDITTFRL
                     IVHVGPATSTQTEGPGPAATSDGGAGATLEPGQTLPPGSGPISGQPPGGCIDFGSCLG
                     QYAIFILIGAVAIVGGGLLAWSRLGGNAAGIPLKAQAPTLGATGSDVAIEPLDLDADK
                     LGSLDADKLGTVDAWKLGDVKLGDGSLSPLEPPGSELPPPPGPTR"
     gene            1146..2168
                     /locus_tag="VIF63_08800"
     CDS             1146..2168
                     /locus_tag="VIF63_08800"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8239520.1"
                     /translation="MDAAIEGARALFVGAQGERASLLARLFRFEATVAAPHARQVLGD
                     ASLNDLVATGLARVADGQVAAQVRLVRFRGALIASDRFGYRRHTEFVIGPGPASALLA
                     DAVRPLERGRVLDLGCGPGSQALWLASGDTDVLGIDISERALAFASFNQRLNGRDRAT
                     FVYGDFLTAPPDPALDERFDVALANPPFVLTPTTELRYRDRPLPGDATTRTALERVAR
                     ALAPGGRGYVLGTWIDAGRGAWDARPRGWLHGLDVRAAIARISSLDPAAYAVAWSRDL
                     EEPARSASIAAWSRALEAEGAARITTGILAIAGPARSSWRRRGQIVALDRVRPLWATF
                     ERALAG"
BASE COUNT          328 a          829 c          783 g          315 t
ORIGIN      
        1 accgagctga ccgtcgccaa cacgacgaca gtcgccgccg ctccccaggc gaccggcggc
       61 cccatcgacg gcccgacagt ggtcaccggc cttgacctcg agatcacctc aaggatcgag
      121 gtccgggcga tgcaggccca gcccgcgcag ggcaccacga gcgccaccgg gctgatcaag
      181 ctcacccgca acgccgacgg cgtgtggtcg gggacgggga acctggcttc gagcaccgag
      241 gtcacgtcca gcgtgtcctg ccagaccacc aacatcaccg gcgacgggac ctacgactgg
      301 gtcgtgcggg atgtccacgc cggccccgag ctcggggcca acttgcgtgt cggcatggac
      361 agcggcccga cgaacgaatc cccggacacc gtgtcgagcg acctctgcgg cacaccctcg
      421 gactcgacga tgaatacgtg ggagaacctc ttcttcgacg cgtaccgtcc gaactacctc
      481 aagaacggcg gcttcctcgt cgatggctgg acaatcctcg atcctgccgc ctggaccacc
      541 ggcggtgccg tcgccgaggc ccactggtcg ggtgcatgca ccggcccgga cctcgggctg
      601 ccgggtatga gcgtcaagct cctggactgc tcggacatca cgaccttccg gctcatcgtg
      661 cacgtcggcc cggcgacatc cacccagacc gagggtccag ggccggccgc gacatccgac
      721 ggcggcgcgg gtgcgacgct cgagccgggg caaacgctgc caccgggcag tggaccgatc
      781 tcgggccagc cacccggagg ttgcatcgac ttcgggtcgt gcctgggcca gtacgcgatc
      841 ttcatcctca tcggcgcagt ggcgatcgtc ggcggtgggc tcctggcctg gtcgcggctc
      901 ggcgggaacg ctgcagggat cccgctgaag gcccaggcgc cgacgctggg cgcaacgggc
      961 tcggacgtcg cgatcgagcc gttggacctt gacgccgaca agctcggctc gctcgacgcc
     1021 gacaagctcg gcacggtcga tgcctggaag ctcggtgacg tcaagctcgg tgacggctcg
     1081 ctatcgccgc tggagccgcc cggctcggag ctgccgcccc cgccaggccc cacccgctag
     1141 cagtcgtgga cgcggcgatc gagggtgctc gagcgctgtt cgtgggtgcg cagggcgaac
     1201 gagcgagctt gctcgcgcgg ctgttccggt tcgaggcgac cgtggctgca ccccacgccc
     1261 gccaggtgct cggcgacgcc tcgctgaatg acctggttgc gaccggcctg gcgcgagtag
     1321 ccgacgggca ggtcgcggcg caggtccggc tcgtccggtt ccgcggcgcg ctcatcgcgt
     1381 cggaccggtt cggctaccgc cgccacaccg agttcgtgat cggcccgggc ccggcctcgg
     1441 cactgctggc cgacgcggtc cggccgctgg agcgaggacg cgtgctcgat ctcggttgcg
     1501 gtccgggcag ccaggcgctg tggctcgcga gtggcgacac cgacgttctc ggcatcgaca
     1561 tcagcgagcg cgcgctggca ttcgcctcgt tcaaccagcg gctcaacggt cgcgaccgcg
     1621 cgacattcgt ctacggcgac ttcctcaccg cgcctccgga cccggccctc gacgagcggt
     1681 tcgacgtcgc gctcgccaac ccgccattcg tgctcacgcc gacgaccgag cttcgatacc
     1741 gggatcggcc gctgcccggc gacgccacga cccgaacggc tctcgaacgc gtggcgaggg
     1801 cactcgcacc gggtggtcgc ggctacgtcc tcgggacctg gatcgatgcc gggcgcggcg
     1861 cgtgggacgc ccgaccgcgc gggtggctcc acggcctcga cgtcagggct gcgatcgcgc
     1921 gcatctcgag cctcgacccc gcggcatacg ccgtggcctg gagccgggac ctcgaggagc
     1981 ccgcacggtc agcgtcgatc gcggcgtggt cgcgcgccct cgaggccgag ggcgcggccc
     2041 ggatcacgac cgggatcctc gcgatcgccg ggccggcgcg atcctcgtgg cgacggcgag
     2101 gtcagatcgt cgcgctcgac cgcgtgcggc ccctgtgggc gaccttcgag cgagcgctcg
     2161 ccggctgagc agggaggacc ttcgacccat cccgccacag cgagccggtg gtagcatgcc
     2221 gctcaactac gactgcggaa gccgactccc atcga
//
LOCUS       DATBSW010000323         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_595367, whole genome shotgun sequence.
ACCESSION   DATBSW010000323 DATBSW010000000
VERSION     DATBSW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595367"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VIF63_08805"
     CDS             complement(<1..413)
                     /locus_tag="VIF63_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY8239521.1"
                     /translation="MTAERVVLTGADLTVARVEAVARRGVEATLDPGARRRMKRSRAV
                     VEKLVADGEVVYGVTTGVGALANRSIGVADVEQLQENLLVSHAAGVGDPLPREVVRAM
                     LVLRANTLALGFSGARPIVAERLLDFLRLGIHPVIP"
     gene            444..1115
                     /locus_tag="VIF63_08810"
     CDS             444..1115
                     /locus_tag="VIF63_08810"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY8239522.1"
                     /translation="MLAFIDQIVIPFLNTLYGAVGYVGVGLAMAIESAMVPLPSELVL
                     PYAGFLISDPSAIEPLTRAHWDFWIVVIIATLGNTLGSIVGYAIGAWGGRPFLERWGK
                     YLLIRPHELELADRFFQKYGPATAFFSRLMPIVRTFISFPAGVARMPLGQFILYSTLG
                     ALPWSILLVWAGQQLGGNWASIREILQPYDLLIAVLGVLLIVGFIWWRLGMPGWRRGE
                     RSAPG"
     gene            1129..1575
                     /locus_tag="VIF63_08815"
     CDS             1129..1575
                     /locus_tag="VIF63_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239523.1"
                     /translation="MKHPRLLPALLSILLVAGCGSSTTSNDSAPPDPCAGVAELGRPI
                     GNPNVPVKFEDAFCPNAVFIATAKLGWLHGPITSTTFRPSVCPPNARCLAALVGVEGW
                     VIFTFAFGDPTMIHVGAPETGAAGLVAGEPERVPDWLIDEVQGVSG"
BASE COUNT          221 a          569 c          537 g          275 t
ORIGIN      
        1 gggatgaccg ggtggatgcc caggcgcagg aaatcgagca gccgctcggc aacgatcggc
       61 cgcgctcccg agaacccgag cgcgagcgtg ttcgcgcgca ggacgagcat cgcccggacg
      121 acctcccgcg gcagcggatc gccaacgccg gccgcgtggc tgacgaggag gttctcctgg
      181 agctgctcga catcggcaac gccgatcgag cgattggcca gcgccccgac gccggtcgtg
      241 accccgtaca cgacctcgcc gtcggcgacg agcttctcga cgacggcccg cgagcgcttc
      301 atccggcgcc gcgcgccggg atcaagggtg gcttccactc cacgtcgtgc gactgcctcg
      361 acgcgggcca cggtaaggtc ggccccggtg aggacgacgc gctctgcggt cacgcaggga
      421 ggatagtcgg ctatcttgcc ccgatgctcg ccttcatcga ccagatcgtc atcccgttcc
      481 tcaatacgct ctacggcgcg gtcggctacg tcggcgtggg gctggcgatg gcgatcgaat
      541 cggccatggt cccgctgccg tcggagctcg tgctgccgta cgccggcttc ctcatctccg
      601 acccgagcgc aatcgagccg ctgacgcgcg cccactggga tttctggatc gtcgtgatca
      661 tcgcgacgct cggcaacacg ctcggctcga tcgtcggcta cgccatcggc gcgtggggcg
      721 gccggccgtt cctcgagcgc tggggcaagt acctgctcat ccgcccgcac gagctcgagc
      781 tcgcggaccg gtttttccag aaatacggcc cggccacggc gttcttcagc cgcctgatgc
      841 cgatcgtgcg cacgttcatc tcgttcccgg cgggcgtcgc gcgcatgccg ctcggccagt
      901 tcatcctcta ttcgaccctg ggcgcgctgc cctggtcgat cctgctcgtc tgggccggcc
      961 agcagctcgg cggcaactgg gcgtcgatcc gggagatcct ccagccgtac gacctgctga
     1021 tcgcggtcct cggcgtgctc ctcatcgtcg gcttcatctg gtggcgcctc ggcatgcccg
     1081 gctggcgccg tggggaacga tcggcgccgg gctgacgtct cggcggcgat gaagcacccg
     1141 cgcctgctgc cggccctgct ctcgatcctg ctcgtcgcgg gatgcgggtc gagcacgaca
     1201 tcgaacgact cggcaccgcc ggatccgtgc gccggcgttg ccgaactggg ccgaccgatc
     1261 ggcaacccga atgtgccggt gaagttcgag gacgcgttct gtccgaacgc ggtctttatc
     1321 gccaccgcca agctcggctg gctccacggc ccgatcacct cgacgacctt ccgaccgagt
     1381 gtgtgcccgc cgaatgcgcg gtgcctcgca gcgctcgtcg gcgttgaggg ctgggtgatc
     1441 ttcacgttcg cattcggcga cccgacgatg attcacgtcg gcgcacccga gaccggcgcg
     1501 gcgggtcttg tcgccggcga gccagagcga gtgcccgact ggttgatcga cgaagtccag
     1561 ggtgtcagcg gctaggttcc ccagacggca tggctctcgt tg
//
LOCUS       DATBSW010000324         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1265070, whole genome shotgun sequence.
ACCESSION   DATBSW010000324 DATBSW010000000
VERSION     DATBSW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265070"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1170)
                     /locus_tag="VIF63_08820"
     CDS             complement(169..1170)
                     /locus_tag="VIF63_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8239524.1"
                     /translation="MDHPTMPSHTSARGSSLALWANMGIVYVVWGSTYFGIAIAIESM
                     PPFLMAAIRFAIAGTILIGWDLIRHPEARHMPTRRQVVDSLIVGGLLLGVANGFVAYG
                     ELTVDSGIAAILVAMMPLWFALFGWIYFRERLPRIVLIAVIVGFIGTALLVAPVGSGA
                     NQFDPVGILILAGAPIAWAHGSMYSQRKAKLPSSPLTASGMQMLAGSLVTAIESVVAG
                     EPANFHPESITPASIAAVVYLVIFGSMVAYTAYAWLLRNAPLSLISTYAYVNPVVAVG
                     LGTIFLHEEISPRTIIGSVVILAAVAIVITARARLQGPSAATSNDEVGRPSVAKAAG"
     gene            1223..2410
                     /locus_tag="VIF63_08825"
     CDS             1223..2410
                     /locus_tag="VIF63_08825"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8239525.1"
                     /translation="MQMIDTAERQRRIGVRHHLAAAARGDDLVTVAGDLVGIHASDPA
                     SVYLGLQARVRELSRDDVAAALYDDRSLLKVLGMRRTMFVVPVPVAGVIHDAAAIDIG
                     ATERKRTIRMVAEAGIAEDAERWLEAVEAETMAVLHELGEATATDLTKRVAGLREQIL
                     FGAGKKWSGRVGVSTRLLFLLAAEGRIIRGRPKGTWLSSLYRWVPVDRWVEGGIEPWP
                     PERAHVELVRRWLIAFGPGTQRDLQWWAGWTVARTKTALRGAGAVEVSLDDGRTGFAL
                     ADDLETTREPKPWVALLPGLDTTTMAWVERDFYLGGHAKQLFDVNGNAGPTVWVDGRV
                     VGLWAQRPDGEVVTRLLEDVGKEREREIDAEAARLDAWLRPDRVFPRFPNPVFQELAA
                     GGA"
     gene            complement(2407..3204)
                     /locus_tag="VIF63_08830"
     CDS             complement(2407..3204)
                     /locus_tag="VIF63_08830"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8239526.1"
                     /translation="MTTLTLTDQALEVALRDLRAIAPASVGFGALVETGLADGYAPVD
                     TPLGPVFVAWNGRGVSWVTPAGDRAEFESRFRASLGRDIAPAELPERIRTAITRRLDG
                     DRRVRIPLDLRGHTPFEVAVWTKALEIPRGEVRPYGWIAAEIGKPKAVRAVGTALAHN
                     PVPLVVPCHRVVRSDGMIGQYSMGGPDAKRRILAAEGLDTRALEAEAAAGHRYTGSDT
                     THIVCHPTCRDARRVTDRHKVTFASLAAATNAGYRPCLHCRPVSLAA"
     gene            complement(3201..3839)
                     /locus_tag="VIF63_08835"
     CDS             complement(3201..3839)
                     /locus_tag="VIF63_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8239527.1"
                     /translation="MDTDIALAKRLANDVDAAFPQLCVAHQDRLYSIALRLLGDRRDA
                     EEVAQDALVRAFRAMREYPPERVADLKLRPWLASITVNLARNRRRRHEDRQPPGSLEP
                     MLDAGFDPPTPGHDTPATSADRRETKRELATALLDLTPAVRSAIVLRHVDGLSVAEVA
                     EALGRPEGTVKSQIHRGLRELRVILEAQPDPISSPHSPARAARLLPALEAIR"
BASE COUNT          608 a         1349 c         1509 g          566 t
ORIGIN      
        1 cgcgggagca tcgtcgccgg cctctacgtc gccgacacct cgacgttccc gacggcgctc
       61 ggggtcaacc ccatggtcac cgtgatggcg atggctcgac gcgtgagccg aacggtgatc
      121 gcggagacgc cgccgagctg agcggggcgg cctggggccg agccgaccct agccggcggc
      181 tttcgcgacg ctcgggcggc cgacctcgtc gttcgacgtc gcggcggacg gcccctggag
      241 ccgcgccctg gccgtgatca cgatggccac ggcggcgagg atcaccaccg agccgatgat
      301 cgttcgcggg ctgatctcct cgtgcaggaa gatcgtgccg agcccgacgg cgacaacggg
      361 gttgacgtag gcatacgtgc tgatcaacga caggggcgcg ttgcgcagga gccacgcgta
      421 cgccgtgtac gcgaccatgc tcccgaagat gacgaggtag acgacggccg cgatcgaggc
      481 cggcgtgatc gactccgggt ggaagttcgc aggctccccg gcgacgaccg actcgattgc
      541 cgtgaccagg gacccggcca gcatctgcat gcccgacgcc gtcagtgggc tcgacggcag
      601 cttcgccttg cgctgtgagt acatcgagcc gtgcgcccac gcgatcggcg cgccggcgag
      661 gatcaggatc ccgaccgggt cgaactggtt ggcgcccgat ccgaccggcg ccacgagcag
      721 cgccgtgccg atgaacccga cgatgacggc gatcagcacg atccgcggga ggcgctcgcg
      781 gaagtagatc cagccgaaga gcgcgaacca caacggcatc atcgccacga ggatcgccgc
      841 gatccccgag tcgaccgtca gctcgccgta cgcgacgaac ccgttcgcga cgcccagcag
      901 caggcccccg acgatcagcg aatcgacgac ctggcgcctg gtcggcatgt ggcgcgcctc
      961 gggatggcgg atcaggtccc agccgatgag gatcgtgccg gcgatggcaa atcggatcgc
     1021 ggccatgagg aacggcggca tcgactcgat cgcgatcgca atgccgaaat acgtcgagcc
     1081 ccagacgacg tagacgatgc ccatattcgc ccagagggcg agggagctcc ctcgcgccga
     1141 ggtgtggctt ggcatagtcg ggtggtccaa gatccggagt ctagcgcagg tcccgcggaa
     1201 ccgcgggata tcctcgagcc cgatgcagat gatcgatacg gccgagcgcc agcgccgaat
     1261 cggcgtccgt catcacctgg cggcggccgc ccggggtgac gacctggtga cggttgcggg
     1321 ggacctcgtc ggcatccacg cgagcgaccc ggcgtcggtc tacctcgggc tgcaggcgcg
     1381 agtccgcgag ctgtcgcgtg acgacgtggc ggctgcgctc tacgacgatc gaagcctgct
     1441 caaggtcctc gggatgcgcc gcacgatgtt cgtcgtcccg gttccggttg ccggcgtcat
     1501 ccacgacgcg gcggccatcg acatcggggc caccgagcgc aagcggacga tccggatggt
     1561 cgccgaagcc ggcatcgccg aggacgccga gcggtggctg gaggcggtcg aggctgaaac
     1621 catggctgtg ctccacgagc tcggtgaagc caccgcgacg gacctgacga agcgagtcgc
     1681 gggcctgcgc gagcagatcc tgttcggtgc cggcaagaag tggtcgggca gggtcggcgt
     1741 gtcgacccgg ttgctcttcc tgctcgcggc cgagggcagg atcatccgcg gccggccaaa
     1801 gggcacgtgg ctgagcagcc tgtatcgctg ggtgccggtc gatcgctggg tcgagggtgg
     1861 catcgagccc tggccgcccg agcgcgccca cgtcgagctg gtgcggcgct ggctgatcgc
     1921 cttcgggccg ggcacgcagc gcgacctcca gtggtgggcg ggctggaccg tcgcccggac
     1981 gaagacggcg ctcaggggcg ccggggcggt cgaggtcagc ctcgacgacg gtcgaaccgg
     2041 gttcgccctt gcggacgatc tcgagacgac ccgcgaaccg aaaccgtggg tcgcgctcct
     2101 gccggggctc gacacgacga cgatggcctg ggtcgagcgc gacttctacc tcggcgggca
     2161 cgcgaagcag ctcttcgacg tgaacggcaa cgccggcccg accgtgtggg tcgacggacg
     2221 cgtcgtcggg ctgtgggcgc agcgcccgga cggcgaggtc gtcacgcggc tgctcgagga
     2281 tgtcggcaag gagcgggaac gggagatcga tgcggaggca gcgaggctgg atgcctggct
     2341 gcggccggat cgcgtgttcc cgcgcttccc gaaccccgtc ttccaggagc tcgcggcagg
     2401 gggcgcctag gccgccagcg acaccggccg gcagtggagg cacggccggt agccggcgtt
     2461 cgttgcggcg gcgaggctcg cgaacgtgac cttgtgccgg tcggtgacgc ggcgggcgtc
     2521 gcggcaggtc gggtggcaca cgatgtgggt cgtgtcggag cccgtgtagc ggtggcctgc
     2581 tgccgcctcg gcctccagcg cgcgcgtgtc caagccctcg gcggcgagga tccggcgctt
     2641 cgcatcggga ccgcccatcg aatactggcc gatcatgccg tcgctgcgga ccacgcggtg
     2701 gcagggcacg acgagcggca ccgggttgtg cgccaacgcc gttccaaccg cgcggaccgc
     2761 cttcggcttg ccgatctcgg cggcgatcca gccgtacggc cgaacctcgc cccgcgggat
     2821 ctcgagcgcc ttggtccaga ccgcgacctc gaacggcgtg tggccacgga ggtcgagggg
     2881 gatccggacc cggcggtcgc cgtccaggcg gcgcgtgatt gccgtccgga tccgctcggg
     2941 cagctcggcg ggcgcgatat cgcgcccgag cgaggctcgg aagcgagact cgaactcggc
     3001 tcgatcgccg gccggcgtca cccacgacac gccgcgcccg ttccaggcca cgaacacggg
     3061 cccgagcggc gtatcgaccg gggcgtagcc gtcggcgagg cccgtctcga cgagcgcgcc
     3121 gaagccgacc gaagccgggg caatcgcgcg caggtcgcgc agcgcgacct cgagcgcctg
     3181 gtcggtgagc gtgagcgtgg tcatcggatg gcctccaggg ccgggagcag gcgagcggcc
     3241 cgcgccggcg agtgtggtga agagatggga tcgggctggg cttcgaggat cacgcggagc
     3301 tcgcgcagcc ctcggtggat ctgcgatttg acggtgccct cgggccgtcc gagcgcttcc
     3361 gcgacctcgg cgacgctcag cccgtcgaca tggcgcagca cgattgccga gcgcaccgcg
     3421 ggcgtgagat cgaggagcgc cgtcgccagc tcgcgcttgg tctcgcgccg gtcagccgag
     3481 gtggcgggcg tgtcgtggcc gggtgtcggc gggtcgaagc cggcgtcgag catcggctcg
     3541 agggagcccg gcggttggcg atcctcgtgc cgccggcggc ggttacgcgc caggttgacc
     3601 gtgatcgatg ccagccaggg ccgaagcttc aggtcggcca ctcgctcagg tgggtactcg
     3661 cgcattgccc ggaatgctcg gacgagcgcg tcctgggcga cctcctccgc gtcgcgccga
     3721 tcgccgagca ggcgcagggc gatcgaatac agccggtcct ggtgtgcgac gcagagctgt
     3781 gggaacgccg catcgacgtc gttggcgagg cgcttcgcga gcgcgatgtc ggtgtccatc
     3841 gaccgtatca acacgccgct cgccggttcg gttgcacgcc ggctgggtgc cgcggcgaac
     3901 gcctactgaa cgacgatgct gcccttcatg gtcgagtgga tgccgcaatg gaatgcgaag
     3961 atcccggcct cggagaagac caggctgtgc gtgccgccgc cggggatgtt cccgtccatc
     4021 ttgcacgagc cg
//
LOCUS       DATBSW010000325         8227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_312733, whole genome shotgun sequence.
ACCESSION   DATBSW010000325 DATBSW010000000
VERSION     DATBSW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312733"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VIF63_08840"
     CDS             <1..849
                     /locus_tag="VIF63_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8239528.1"
                     /translation="SGRRSPVPIPGSEFVIKADTVIPAVSQAADLTFLPVEADFEVNR
                     GRVKVDPATYATNVRGVFACGDFVTGPTTLIEAAGHGKKCAYAIDRYLAARTDVTVAA
                     NVKMTSTWTFEMPEFYDVLPRQHIPMTALPDRMPSTDPDVNFTTQVERGYDATAAVAE
                     STRCLMCNFNIWFDPFRCVLCGACADVCPEGIIHMVDINTLKSEGFLTELDEAYGWQD
                     GAAMILDEERCIRCALCVKRCPFDAITMERFELQEISTDGRLYKESYRDAQLAGTPGV
                     AGGMAR"
     gene            846..1574
                     /gene="extP"
                     /locus_tag="VIF63_08845"
     CDS             846..1574
                     /gene="extP"
                     /locus_tag="VIF63_08845"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEY8239529.1"
                     /translation="MSFLERVDQTIRRSPVWRSLFRNPYPTDERSRAYAVMNNVFLHL
                     HPVRVRQHAVKFAYTFCLGGLSFFLFLVLTVTGVYLMFFYIPSTTSAYTDILNIQASV
                     AFGAITRNVHRWGAHLMVFFVFLHMMRVFYHGAYKPPREFNWVIGVLLLLMTIALSFT
                     GYLLPWDQISYWAITIGLNMASYTPVINVPIQNLIFGGLEVGQNTLLRFYVLHIMVLP
                     LVAAILLAVHFWRIRKDGGISGPL"
     gene            1578..2195
                     /locus_tag="VIF63_08850"
     CDS             1578..2195
                     /locus_tag="VIF63_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239530.1"
                     /translation="MTDQKTESEKRVVPVDPTRRTEIDKQRVPMSARPYRLLAMVKQD
                     AVVRVQKEPDDTVMTWPHLLSIEFLAATVMSIFLLLMGLFINAPLEELANGNVTPPVA
                     KAPWYLLGLQEMLAYFHPVISGVLVPFLYILLGAIVLPYIDRANIEAKRPAERKTAVV
                     LFSLLCVLGLTVTFVAVFFRGPGYSLVTPYLPFFDNFTNGLHFAL"
     gene            2203..2877
                     /locus_tag="VIF63_08855"
     CDS             2203..2877
                     /locus_tag="VIF63_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8239531.1"
                     /translation="MSNYHLQPADKPQALTPQALRSLRQDPVQGISRRTLLRRSIGGA
                     TLLWLTEVAAGSIGFLWPNSKGGFGSVIEIGTIAEIKAQNSSLPFNNGFPVYYQNARA
                     YIMLIDPARQEFVPGEDKTGEGTALNVRALYQRCPHLGCKPNPCLKNYWFECPCHGSR
                     YDRLGIKAQGAQYGPAPRGMDRFSITVKADGKLVLDTSKITLGPLPVAVGQPGIIPPK
                     SPVGCI"
     gene            2874..3935
                     /locus_tag="VIF63_08860"
     CDS             2874..3935
                     /locus_tag="VIF63_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8239532.1"
                     /translation="MTDQTGDRPEGRPERRLPARRPPSDTVPSRSADRFTSPRQVRNI
                     HGLTSERAAKIVRQTADARWVAFLGVVIVALFVIIYYFYELGVPIINTTPRLEAEGKL
                     QQVTSVERGYNLYEANCARCHGVNGEGGIGPILNDQSKLFSHLTESYIRNVLTVGGRY
                     VCGDPKSVMPVWANTNGGPLNYIAIDDLIAFIRAPSTQAYTRRDPEFNEPILGADGKV
                     LMFKGWRDEAFKPEPSATAVPDCYKGTAGGATAAPPASLPPDATVLELTAVDVEFDKH
                     ELSAPAGKVFAVDFTNDGSLEHDMDIRLAAGQPTLVDDPKITNGATQFVIGPLEAGTY
                     NFFCSVHDIPGMNGTLTVK"
     gene            3938..5470
                     /locus_tag="VIF63_08865"
     CDS             3938..5470
                     /locus_tag="VIF63_08865"
                     /inference="COORDINATES: protein motif:HMM:NF025223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239533.1"
                     /translation="MAKALPAGRAMRRRVVFGFFDADGWAWAFTKAMFWLLVIIMALG
                     YIPDRAYYFFVSRTIELGILGWSPINLCPPENGTSMPCPVPAGGVLPWQAAPAEAALP
                     QPRAGGVALQLGKNLIYAGGSDASGPTATSYLAQIDKDKFGPWSDGPALPEARTNAGL
                     ATLSGTAYLIGGAGPDGATTTVWRLGLDPDTSALKTWEPVCTVAETKEKPCPVDKLLA
                     LPAARSGAAVVAVSDGIVVAGGLGPDGKPTNTVWKATADPKSGILTAFAEQPGLPLAV
                     SDASAVFEGTFLWVYGGSDAAGPVGAVQRADYGSAPGPTAEPGAPAPSAAPEVVLKWT
                     ADNALNLPAGAARTGAAGFAANGALYLVGGSDGTSAKRELFWALPNSAGNLPGGWRHL
                     DATDLPRGIADAAPLVTGATAVLIGGIGPDGPLATSMKVSLAPQEPFFRVGLVGVVVP
                     GLRIGGEIGQQLGYLAAAGVGTGNFVILVAVGWAFNHREDIKAWRDRRRIAKEAKAPK
                     EG"
     gene            complement(5693..6055)
                     /locus_tag="VIF63_08870"
     CDS             complement(5693..6055)
                     /locus_tag="VIF63_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEY8239534.1"
                     /translation="MPAAARRAAFSTGLAAYDRGDFFLAHELLEPPWMGARDLAEREL
                     IQGVIKLAAAFVHGARSNPAGIEKNLRGARERLAAGVEAGPRLGLDVEELVRRVDAWL
                     KAPGGPPISIPRGHSVDS"
     gene            complement(6139..6576)
                     /locus_tag="VIF63_08875"
     CDS             complement(6139..6576)
                     /locus_tag="VIF63_08875"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY8239535.1"
                     /translation="MSRFYAIDEANEALPEVERILSDLRDQREELIELRDRVVAASPP
                     DEEVAPPGVAEQVRLLRLGMQGLIDQMQAGVARLIEMDVTLRDISTGLIDFPALVSGM
                     PIWLCWRLGEDDVAHWHPHDEGYDTRRPLSELPMGRGSGTPAA"
     gene            6610..7806
                     /locus_tag="VIF63_08880"
     CDS             6610..7806
                     /locus_tag="VIF63_08880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8239536.1"
                     /translation="MKPATRMATIGTESAFEVSARARALEATGRSIVYLQIGEPDFDT
                     PANVREAAKAAIDAGMTHYPPFAGLPDLRAAIAADTTARRGFAVEPSNVFVTVGGKGV
                     MVYAIMGLVDPGDEVIVPDPGYPIYESITSFVGGKPVPIPIRMENDFRLDVDELATLI
                     TPRTKLLVINSPANPTGGVLTRNDIERIAELALRHDLVVLSDEIYGRILYDGAEHVSI
                     ASLDGMAERTILLDGFSKTFAMTGWRLGYAVVPDWLVKTYSQLIINTISGVTAFAQAG
                     AIEALRGPQDDVDAMVVEFLARRDLVVDGLNAIPGIRCHRPQGAFYVFPDISGTGLTG
                     ADLSERLLQEAGVCVLPGTAFGGVGTNHIRISYANSRENLTEALARIRRFVEPLVEAR
                     ATAAPA"
     gene            7807..>8227
                     /locus_tag="VIF63_08885"
     CDS             7807..>8227
                     /locus_tag="VIF63_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239537.1"
                     /translation="MAQPRVFVSRIIPEDGLRPIRDMCDATVWEDELPPPRDALLREI
                     AGCDGVLTLLTDKVDDELLDAAGPQLKVVSDLAVGYDNVDVPACTRRGIAVGNTPGVL
                     TETTADLAFGLLMAGARRIAEGDRLVRSGGWRTWGPLA"
BASE COUNT         1271 a         2983 c         2678 g         1295 t
ORIGIN      
        1 tccggccgcc gctcgcccgt gccgatcccc ggctccgaat tcgtgatcaa ggccgacacg
       61 gtcatcccgg ccgtgtccca ggcggcggac ctcaccttcc tgccggtcga ggcggacttc
      121 gaggtcaacc gcggccgggt caaggtcgat cctgcgacgt acgcgacgaa cgtccgcggc
      181 gtcttcgcct gcggcgactt cgtgaccggc ccgacgaccc tcatcgaggc cgccggccac
      241 ggcaagaaat gcgcctacgc gatcgatcgc taccttgcgg cccgcaccga tgtgacagtc
      301 gcggcgaacg tgaagatgac gagcacctgg acattcgaga tgcccgagtt ctacgacgtc
      361 ctgccgcgcc agcacatccc gatgaccgcg ctgccggacc ggatgccctc gaccgatccc
      421 gacgtcaact tcacgaccca ggtcgagcgc ggctacgacg cgacggcggc cgtcgccgag
      481 tcgacgcgct gcctgatgtg caacttcaac atctggttcg acccgttccg gtgcgtgctc
      541 tgtggcgcct gcgccgacgt gtgccccgag ggcatcatcc acatggtcga catcaacacc
      601 ctcaagtcgg agggcttcct caccgagctc gacgaggcct acggctggca ggacggcgcg
      661 gcgatgatcc tcgacgagga gcgctgcatc cgctgcgcct tgtgcgtcaa gcgttgcccg
      721 ttcgacgcca ttacgatgga gcggttcgag ctccaggaga tctcgaccga cggccggctc
      781 tacaaggagt cgtaccggga cgctcagctc gccggcacgc ccggtgtcgc aggaggaatg
      841 gcccgttgag cttcctcgag cgggtggatc agaccatccg ccgaagcccc gtctggcgct
      901 cgctgttccg caacccgtat ccgacggacg agcggtcgcg cgcctacgcg gtcatgaaca
      961 acgtgttcct gcacctgcac ccggtgcggg tccgccagca cgcggtcaag ttcgcgtaca
     1021 cgttctgcct gggtggcctg tcgttcttcc tgttcctcgt cctgaccgtc accggcgtct
     1081 acctgatgtt cttctacatc ccgtcgacga cgtcggccta cacggacatc ctgaacatcc
     1141 aggcgagcgt tgcgttcggc gcgatcacgc ggaatgtcca tcggtggggc gcccacctga
     1201 tggtgttctt cgtcttcctg cacatgatgc gggtcttcta tcacggggcc tacaagccgc
     1261 cgcgcgagtt caactgggtc atcggcgtgc tgctgctgct gatgaccatc gccctgagct
     1321 tcaccggcta cctgctgcca tgggaccaga tcagctattg ggcgatcacg atcggcctga
     1381 acatggccag ctacacgccc gtcatcaacg tcccgatcca gaacctcatc tttggtggcc
     1441 tggaggtcgg gcagaacacg ctgctccggt tctacgtgct ccacatcatg gtgttgccgc
     1501 tggtcgcggc catcctcctc gcggtgcact tctggcgcat ccgcaaggac ggcggcatct
     1561 cggggcctct gtgagccatg actgaccaga agaccgagtc cgagaagagg gtggtcccgg
     1621 tcgacccgac ccggcggacc gagatcgaca agcagcgcgt gccgatgtcg gcgcggccgt
     1681 accgcctgct cgcgatggtc aagcaggacg cggtcgtccg agtccagaag gaacctgacg
     1741 acacggtcat gacctggccg cacctgctgt cgatcgagtt cctggctgcg accgtcatga
     1801 gcatcttcct gctgctcatg ggcctgttca tcaatgcccc gctcgaggag ctggcgaacg
     1861 gcaacgtgac cccgcccgtc gcgaaggcgc cgtggtacct gctgggcctc caggagatgc
     1921 tggcctactt ccaccccgtc atctcgggcg tgctcgtgcc cttcctgtac atcctcctcg
     1981 gcgcgatcgt cctgccctac atcgatcggg cgaacatcga ggcgaagcgc ccggccgagc
     2041 gcaagacggc ggtcgtgctg ttcagcctgc tgtgcgtcct cggtctgacc gtgacctttg
     2101 tcgctgtctt cttccgcggc cctggctact cgctggtgac gccgtacctg ccgttcttcg
     2161 acaacttcac caacggcctc cacttcgcgc tgtgaccgac cgatgagcaa ctaccacctc
     2221 cagcccgccg acaagccgca ggcgctcacg ccccaggcgc tgcgctcgct ccgccaggat
     2281 cccgtgcagg gcatttcgcg ccggaccctg ctgcgccgct cgatcggtgg tgcgacgctg
     2341 ctctggctga ccgaggtcgc cgccggctcg atcggcttcc tgtggccgaa ctcgaagggc
     2401 ggcttcggca gcgtcatcga gatcgggacc atcgccgaga tcaaggccca gaactcgtcg
     2461 ctgccgttca acaacggctt ccccgtctac taccagaacg cccgcgccta catcatgctc
     2521 atcgacccgg cccgccagga gttcgtgccc ggcgaggaca agaccggcga gggcaccgcg
     2581 ctcaacgtcc gcgccctgta ccagcgctgc ccgcacctag gctgcaagcc caacccgtgc
     2641 ctcaagaact actggttcga gtgcccgtgc cacggctcgc gctacgaccg cctcgggatc
     2701 aaggcccagg gcgcgcaata cggccccgcg ccgcgcggca tggaccgctt ctcgatcacg
     2761 gtcaaggctg acgggaagct cgtcctcgac acgtcgaaga tcaccctcgg cccgctgccg
     2821 gtcgcggtcg gccagcccgg catcatcccg ccgaagagcc ccgtcggctg catatgaccg
     2881 atcagaccgg cgaccggccc gagggccggc cagagcggcg gctgcccgcc cgccgcccac
     2941 cgagtgacac cgtcccgagc cgctcggccg atcgcttcac ctcgccccgc caggtccgga
     3001 acatccacgg gctgacgtcg gagcgagcgg ccaagatcgt ccgccagacc gccgacgcgc
     3061 gttgggtcgc gttcctcggc gtggtgatcg tggccctgtt cgtgatcatc tactacttct
     3121 acgagctggg cgtcccgatc atcaacacga cgccgcgcct cgaggccgaa ggaaagctcc
     3181 agcaggtcac gtccgtcgag cgcggctaca acctgtacga ggcgaactgc gcgcgctgcc
     3241 acggcgtcaa cggcgagggc ggcatcggac cgatcctcaa cgaccagtcg aagctgttca
     3301 gccacctgac cgagtcctac atccgcaacg tgctgaccgt gggcggccgg tacgtgtgcg
     3361 gcgacccgaa gagcgtcatg ccggtctggg ccaacacgaa cggcgggccg ctcaactaca
     3421 tcgcgatcga cgacctcatc gcgttcatcc gggccccgag cacgcaggca tacacgcgtc
     3481 gcgacccgga gttcaacgag ccgatcctgg gcgcggacgg caaggtcctg atgttcaagg
     3541 gctggcggga cgaggcgttc aagccggagc cgagcgcgac cgccgttccc gattgctaca
     3601 agggcaccgc cggcggggcc acggccgcgc cgcccgcgag cctgcccccg gacgccacgg
     3661 tcctcgagct cacggccgtt gacgtggagt tcgacaagca cgagctctcc gcgcctgccg
     3721 gcaaggtgtt cgccgtcgac ttcacgaacg acggcagcct cgagcacgac atggacattc
     3781 gactcgcggc cggccagcca acgctggtgg acgacccaaa gatcacgaac ggtgcaaccc
     3841 agttcgtgat cgggccgctc gaggccggca cctacaactt cttctgctcg gtgcatgaca
     3901 tccccggcat gaacggcacc ctgacggtga aatagggatg gcgaaggcac tcccggccgg
     3961 tcgcgccatg cggcgtcgcg tcgtcttcgg cttcttcgat gccgacggct gggcgtgggc
     4021 gttcacgaag gccatgttct ggctcctcgt gatcatcatg gccctgggct acatcccgga
     4081 ccgcgcctac tacttcttcg tctcacgcac gatcgagctc ggcatcctcg gctggtcgcc
     4141 gatcaacctg tgcccgcccg agaacggcac gtcgatgccc tgcccggtgc cagccggcgg
     4201 cgtcctgccc tggcaggcgg cgcccgccga ggccgcgttg ccccagccac gggccggcgg
     4261 cgtcgcgctc cagctcggaa agaacctcat ctacgccggc ggttcggacg cgtcaggccc
     4321 gacggccacc tcctacctcg cccagatcga caaggacaag ttcgggccat ggtccgacgg
     4381 tcccgcgctg ccagaggcac gcacgaatgc cggcttggcc acgctgagcg gcacggccta
     4441 cctgatcggt ggcgccggac ccgatggcgc gacgaccacg gtctggaggc tcgggctgga
     4501 cccggacacg agtgccctga agacgtggga gcctgtgtgc acggtcgccg aaaccaagga
     4561 gaagccgtgc cccgtggaca agttgctggc actgccggcg gcgcggtccg gcgcggccgt
     4621 ggttgccgtt tcggacggga tcgtcgtcgc cggcgggctc ggacccgacg gcaagccgac
     4681 caacaccgtg tggaaggcaa ccgccgatcc caagagcggc atcctcaccg ccttcgccga
     4741 gcagcccggc ctgcctctcg ccgtgtctga cgccagcgcc gtcttcgagg gcacgttcct
     4801 ttgggtgtac ggcggcagcg acgctgccgg tccggtcggc gctgtgcaac gcgccgacta
     4861 cggttccgcg cccgggccga cggccgaacc gggcgcgcct gccccgtcgg cggcgcccga
     4921 ggtcgtcctc aagtggaccg ccgacaacgc gctgaacctg cccgcgggcg cggcgcgaac
     4981 cggcgctgcc ggcttcgccg cgaacggcgc gctatacctc gtcggcggct ccgacggcac
     5041 cagcgcgaag cgagagctgt tctgggccct gccgaactcg gcgggcaatc tgccaggcgg
     5101 ctggcgccat ctcgacgcca cggacctgcc caggggcatt gccgatgccg cgccgctcgt
     5161 gaccggcgcg accgcggtgc tgatcggcgg catcgggccg gacggcccgc tcgcgacgtc
     5221 gatgaaggtg agcctggcgc cccaggagcc gttcttccga gttggcctgg tgggcgtcgt
     5281 cgtgcccggc ctcaggatcg gtggcgagat cggccagcag ctcggatacc tggccgcagc
     5341 cggcgtgggc acgggcaact tcgtgatcct ggtcgcggtc ggctgggcgt tcaaccaccg
     5401 cgaggacatc aaggcgtggc gggaccgccg ccgcatcgcc aaggaagcca aggcgcccaa
     5461 ggagggctag gcggcacgcc tttcgagtct cgatcgcccg atgtcgattc ggtcatccgt
     5521 tactcacccg ggcgctagtc acgaccatcg agtcaaatgg gcgcctcggg atacatctgc
     5581 gcctggtgaa cggcccgtgc gtgcggggag cccccagcgg tgcgtcgcga tggctgacgt
     5641 gtgcccgggg cagtaacacc tgacacattc gatggtgccc gcgagcctct gctcagctgt
     5701 cgacggaatg gcctcgcggg atcgagatcg gcggcccacc gggtgccttg agccaggcat
     5761 cgacccggcg gacgagctcc tcgacgtcga ggccgagccg cggccccgcc tcgacacctg
     5821 ctgcaaggcg ttcccgcgcg ccgcgcaggt tcttctcaat gccggccggg ttcgagcgtg
     5881 ctccatgcac gaatgccgcc gcgagcttga tgacgccctg gatgagctcg cgctcggcaa
     5941 ggtcgcgagc acccatccac ggcggttcga gcagctcgtg ggcgaggaag aagtcgcccc
     6001 ggtcgtacgc cgcgaggccg gtcgagaagg ccgcgcgccg ggccgcggca ggcatcggcc
     6061 ggtacgcctt ggcccgaccg ccctggctga tggtgcgagc cgcaggcccg cttcgtggca
     6121 ctcggctgga gccggtcgtc aggccgccgg agttccggag ccgcggccca tcggcagctc
     6181 gctcaggggg cggcgcgtgt cgtagccctc gtcgtgcggg tgccagtgcg cgacgtcgtc
     6241 ctcgccgagc cgccagcaca gccagatggg catgccgctg acgagcgccg ggaagtcgat
     6301 caagccggtc gagatgtcgc gcagggtgac gtccatctcg atgaggcgcg cgacgccggc
     6361 ctgcatctgg tcgatcaggc cctgcatccc gagccgcagc aggcgcacct gctcggcgac
     6421 gccgggcggc gccacctctt catccggcgg cgacgcggcg accacgcggt cgcgcagctc
     6481 gatcagctcc tcacgctggt cgcgcaggtc cgacaggatc cgctcgacct cgggcagcgc
     6541 ctcgttcgct tcgtcgatgg catagaaccg gctcaccgat cgattttagg ccaccctaga
     6601 atgaacgcca tgaaaccagc cacgcgcatg gcgacgatcg gcaccgaatc cgccttcgag
     6661 gtgtccgcca gggcgcgagc gctcgaggcg accggtcgat cgatcgtcta tctccagatc
     6721 ggcgagcccg acttcgacac gcccgcgaac gtgcgcgagg cggcgaaggc cgccatcgac
     6781 gccgggatga cgcattaccc gcccttcgcc ggcctgcccg acctgcgcgc cgccatcgcc
     6841 gccgacacga ccgcgcggcg cggcttcgcg gtcgaaccgt cgaacgtttt cgtcaccgtc
     6901 ggcggcaagg gcgtgatggt ctacgcgatc atgggcctgg tcgacccggg cgacgaggtc
     6961 atcgtccccg atccgggtta cccgatctac gaatcgatca cgagcttcgt cggcggcaag
     7021 cccgtcccga tcccgatccg gatggagaac gacttccggc tcgatgtcga cgagctcgcg
     7081 accctgatca cgcccaggac aaagctcctc gtgatcaatt cgccggcgaa tcccaccggc
     7141 ggcgtgctga cccgcaatga catcgagcgc atcgccgagc tcgcgcttcg ccacgacctc
     7201 gtcgtcctca gcgacgagat ctacgggcgg atcctgtacg acggcgcgga gcacgtgtcg
     7261 atcgcctcgc tcgacggcat ggccgagcgc acgatcctcc tcgatggctt ctcgaagacc
     7321 ttcgcgatga ccggctggcg cctcggctac gcggtcgtgc ccgactggct cgtcaagacg
     7381 tacagccagc tgatcatcaa caccatttcc ggcgtcaccg cgttcgccca ggcgggcgcg
     7441 atcgaagcgc tgcgcgggcc gcaggacgac gtcgacgcga tggtcgtcga gttcctcgcc
     7501 cggcgcgacc tcgtcgtcga cgggttgaat gccatcccgg gcattcgctg ccatcggccg
     7561 cagggcgcgt tctacgtctt ccccgacatc tccggcactg ggctcaccgg cgcggacctt
     7621 tccgagcggc tgctccagga ggcgggcgtc tgcgtcctgc cgggcacggc gttcggcggc
     7681 gtcggcacga accacatccg gatctcgtac gccaactctc gcgagaacct caccgaagcg
     7741 ctggcgcgga tccgccgctt cgtggagccg ctcgtcgagg cccgcgctac tgcagctccg
     7801 gcgtaaatgg cgcagccgcg ggtcttcgtc tcgcggatca tccccgagga cgggctgcgc
     7861 ccgatccgcg acatgtgcga cgcgacggtc tgggaggacg agctcccgcc gccgcgcgac
     7921 gcgctcctcc gcgagattgc gggctgcgac ggcgtcctga cgctcctcac cgacaaggtc
     7981 gacgacgagc tgctcgacgc ggccggccct cagctcaagg tcgtgagcga cctggccgtc
     8041 gggtacgaca acgtggacgt gcccgcctgc acccggcggg gcatcgcggt cggcaacacg
     8101 cccggcgtgc tgaccgagac caccgccgac ctcgcgttcg gcctcctcat ggccggcgcg
     8161 cgccggatcg ccgagggcga tcgcctcgtc cgctccggcg gctggcggac ctggggcccg
     8221 ctggcgt
//
LOCUS       DATBSW010000326         8257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_595727, whole genome shotgun sequence.
ACCESSION   DATBSW010000326 DATBSW010000000
VERSION     DATBSW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595727"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /gene="dnaE"
                     /locus_tag="VIF63_08890"
     CDS             complement(<1..1848)
                     /gene="dnaE"
                     /locus_tag="VIF63_08890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8239538.1"
                     /translation="MTTLASNDFVHLHTHSEFSLLDGLGRITELVDEANTKGFDSLAI
                     TDHGALYGAVAFYQAATNKGIKPIIGVETYVARRSMTDKEGKADAQPFHLILLASDLT
                     GYRNLCRLVTDAHIDGYYYKPRIDREHLAKHSEGLIGLSACLNGEVSRALEVDDWDLA
                     RTVAGEYSDIFGKDRFFLELQDHGLQEQRRLNEQLLRLAPETGLPMVVTNDLHYVRRE
                     QSAAHDVLLCVGTASNLDTPGRLKFETDDFFLKSAADMAALFPDNLEAITNSRRIAEM
                     CDVKLPLNQLRIPHFPVPDGETVESWLKAECQRGLAWRYGTVTEALQTRLDYELGVIN
                     SMGYAGYFLIVADFIRFAREQGIQTTCRGSAPGSIVTYTLGITPVDPIAYELPFERFL
                     NPDRVTMPDIDVDFQDDRRDEVIAYVSRKYGQDHVAQIITFGTMLARAAIRDVGRVLG
                     HSYGEVDRIAKAVPNQLGIKLDEALESAPQLRELYDADPGVKRTIDFAKQLEGVARNA
                     STHAAGVVISREPLTELMPLQKATNSDSLMTQYEMHAIEALGLLKFDFLGLSNLTILK
                     NAVDLIKAHRGIDVDLDNIPLDDTKTFDLLSTGETTGIFQLESAGMRRYV"
     gene            1890..2813
                     /locus_tag="VIF63_08895"
     CDS             1890..2813
                     /locus_tag="VIF63_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8239539.1"
                     /translation="MVDFGISLAPPWAEPMHDRHLWYLDLLRRGEGAFTSAWVSDHLM
                     KNDHPVVEAWTTIAYLAAEAPSYRFGNLVLSQSYRNPALLAKMAATLQYLTGGRLILG
                     IGAGWQADEYEAYGYEYPSAGVRVEQLAEAIDVLRAMWTQSPATYEGKHYRVRDAYCE
                     PRPEAPIPILVGGRRPKFMRVAAEKADIWQWDGPIERYRVPYDLLVANCAGVGRDLAS
                     VTLSTFGEVYFPEDPADFPREPLLAFTDAAQDPSGAYAGEADWVLGPTPADAVAALRP
                     LVDLGVGQVTIYFWDMRSLDLFIREVIPAFR"
     gene            complement(2823..3008)
                     /locus_tag="VIF63_08900"
     CDS             complement(2823..3008)
                     /locus_tag="VIF63_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239540.1"
                     /translation="MRTYAPADLKVAAGLLADVVDEVGQSILAGNPAPEILTQGQSPE
                     RIKALGDVSKWIDANCS"
     gene            complement(3426..4124)
                     /locus_tag="VIF63_08905"
     CDS             complement(3426..4124)
                     /locus_tag="VIF63_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEY8239541.1"
                     /translation="MSVELAAAILLILVPIAFNVAFFELARSFDYPDILRREPDEILR
                     RFSAGGAGLILRWEALLVSALAMLPLAVIVALAIGADSVLTGLSIVVGAAAALVQGLG
                     LVRWPFAVPELARRYIAADEGPAGDPTRQSVEVTFAMLHRLLGVGIGEHLGYLFTGLW
                     TLLIAASILTTTVLPAWLGIVGLPIGIALLIGTLEFVGPNERDGWKLAGTIVPIAYIA
                     WSLWLIALGVFLLL"
     gene            complement(4140..4784)
                     /locus_tag="VIF63_08910"
     CDS             complement(4140..4784)
                     /locus_tag="VIF63_08910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY8239542.1"
                     /translation="MSPAGPSAGAPSSGAGGTARLRYVALGDSFTIGTSVTAQERWPD
                     QLARRLPVLELVANLGVNGFTSSDVIELELPQIATLDPDLVTLLIGVNDVVQGVPEAT
                     YRANVRVILDALAAQVGADHVLVVTTPDYTVTPAGADYGDPTAQSAKIRAYNAINTEL
                     ATARGIAVADIYDISLGAATDRSLVASDGLHPSGAQYALWVDVIAPAITSLAGR"
     gene            complement(4897..6174)
                     /locus_tag="VIF63_08915"
     CDS             complement(4897..6174)
                     /locus_tag="VIF63_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8239543.1"
                     /translation="MTAVRVGGEGVVDRAAVRRQRILGVTYLVAAAIAAFVLTSDIDA
                     DAISTFQLKPAGNQGGLDVPNIVVPSVLTQYVIAALCAALGAMQLIRGFGSRWAVVLG
                     VVVVAFVFGFLTWAATGRSMSLIGILQLTVVKALPITFGALSGLLCERAGVINIAIEG
                     MLLSGAFTGAVVGSATGNNIIGLAGAALVGGSLGALLAVLAIRYRMDQIIAGTVINIF
                     AAGITAFAAARVLQSHRELNEPEKFASLAIPVLSDIPVLGPMFFNGNIFLYALFVLIG
                     LLHYGLFYTRWGLRVRAVGEHPRAADTVGINVLLTRYRNVIFAGVVAGVGGAYFTLGS
                     TGSFVQNMTGGRGFIGLAAMIFGGWTPIGSFLAGIVFGFADALQARLSILNVPVPSEF
                     LLMVPYIVTIIVVAGLVGRVRAPAADGQPYDKG"
     gene            complement(6171..7334)
                     /locus_tag="VIF63_08920"
     CDS             complement(6171..7334)
                     /locus_tag="VIF63_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8239544.1"
                     /translation="MVPVLAIFSALIIGAFVIVLSDLTFFERLSQDPAAALGLALDSV
                     LKAYYALLTGAVGNPGTYIDAFAAGDAGQITKAFAPISETLLAATPLIFTGLAVALGF
                     QSGLFNIGADGQLYVGAAFATFVGFAVTGVPWFIHLPLAMGAGFLGGALWGFIPGILK
                     ARTGAHEVIVTIMLNYTAYRLIEFLLKVPGYQREGRSDPISKVTEASSWYPALVEGLR
                     ANWGFVIGLVAALAVSWLLFRSTRGFEFRAVGFNPHAARYAGMSIASSTVLAMAISGG
                     LAGLAGTSIVLGVTRSLTSGISAGLGFDGIAIALIARSRPRGIIFAALLFGALRAGAT
                     PMQSLTGVPIDLVVVIQALVIMFVAAPALVRGIYRIRVPETTGTETFSKGWGS"
     gene            complement(7485..>8257)
                     /locus_tag="VIF63_08925"
     CDS             complement(7485..>8257)
                     /locus_tag="VIF63_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8239545.1"
                     /translation="DDRGHLAVNGLDLSVRAGEIVGIAGVQGNGQTELVEAITGLREP
                     VAGTITYEGKVVKLEPRAVLEAGVAYIPEDRLEDGCVPGFSIAENLVLNTYYNAPFSD
                     GLRLDRDAIATSAHKLVKEFDVRTTSVFNDISNLSGGNQQKVIVAREFSRPIHLLVAA
                     QPTRGLDVGSIEYIHRRIVEKRDEGTAVLIVSTELDEVMALADRIAVMYQGRIVDVLD
                     VEQATPQLLGLLMGGSGLVSDAQAGSAGLATEAVSPDA"
BASE COUNT         1379 a         2708 c         2890 g         1280 t
ORIGIN      
        1 gacataccgc cgcatccccg ccgactccag ctggaagatg cccgtcgtct cgcccgtgct
       61 caggagatcg aacgtcttcg tgtcgtcgag cgggatgttg tcgaggtcga cgtcgatgcc
      121 ccggtgagcc ttgatcaggt cgaccgcgtt cttgaggatc gtgaggttcg agaggccgag
      181 gaagtcgaac ttgagcaggc ccaatgcctc gatggcgtgc atctcgtact gggtcatcag
      241 cgagtccgag ttcgtcgcct tctggagcgg catcagctcg gtgagcggct cgcgcgagat
      301 cacgacgccc gcggcgtggg tggacgcgtt ccgtgcgacg ccctcgagct gcttcgcgaa
      361 gtcgatcgtg cgcttcaccc cggggtcggc gtcgtacagc tcgcggagct gcggcgccga
      421 ttcgagcgcc tcgtcgagct tgatgccgag ctggttcggg accgccttgg cgatccggtc
      481 gacctcgccg tacgagtggc ccagcacgcg cccgacgtcg cggatcgcgg cgcgggcgag
      541 catcgtgccg aaggtgatga tctgggcgac gtggtcctgg ccgtacttgc gcgagacata
      601 ggcgatgacc tcgtcgcgcc ggtcgtcctg gaagtcgacg tcgatgtcgg gcatcgtcac
      661 gcgatcgggg ttcaggaagc gctcgaacgg gagctcgtag gcgatcgggt cgaccggcgt
      721 gatgccgagc gtgtacgtca cgatcgagcc cggggcggag ccgcggcagg tcgtctggat
      781 gccctgctcc cgcgcgaatc ggatgaagtc cgcgacgatc aggaagtagc cggcgtagcc
      841 catcgagttg atgacgccga gctcgtagtc gaggcgggtc tggagggcct cggtgaccgt
      901 cccgtagcgc cacgcgaggc cgcgttggca ctccgccttg agccaggact cgaccgtctc
      961 gccgtcggga accgggaagt gcgggatgcg cagctggttg agcggcagct tgacgtcgca
     1021 catctcggcg atgcggcgcg agttcgtgat cgcctcgagg ttgtcgggga agagcgcggc
     1081 catgtcggcg gcggacttca ggaagaaatc gtcggtctcg aacttgagcc ggccgggcgt
     1141 gtcgaggttc gacgccgtgc cgacacagag cagcacgtcg tgggcggccg actgctcccg
     1201 gcggacgtag tgcaggtcgt tcgtgacgac catcggcagg cccgtctcgg gagcgaggcg
     1261 cagcagctgc tcgttgagcc ggcgctgctc ctggagccca tgatcctgca gctcgaggaa
     1321 gaaccggtcc ttgccgaaga tgtccgagta ttcgccggcg acggtgcggg cgaggtccca
     1381 gtcgtcaacc tcgagcgcgc gcgagacctc gccattcagg cacgccgaca gcccgatcag
     1441 gccctcgctg tgctttgcca ggtgctcgcg gtcgatgcgc ggcttgtagt agtagccgtc
     1501 gatatgcgcg tcggtgacga gccggcacag gttgcgatag ccggtcaggt cgctcgcgag
     1561 caggatcagg tggaagggtt gggcgtcggc cttgccctcc ttgtcggtca tcgaccggcg
     1621 ggcgacgtac gtctcgacgc cgatgatcgg cttgatgccc ttgttcgtcg cggcctggta
     1681 gaacgcgacg gcgccgtaca gcgcgccgtg atcggtgatg gcgaggctgt cgaagccctt
     1741 cgtgttcgct tcgtcgacca gctccgtgat ccgtcccagg ccgtcgagga ggctgaactc
     1801 cgagtgggtg tggagatgga cgaaatcgtt cgaagcgagg gtcgtcacgg ggggccaatg
     1861 gtagcccgcg ggctcgccat acttcgccga tggtcgactt cgggatctcc ctcgctcctc
     1921 cgtgggcgga gccgatgcac gaccgccatc tctggtacct ggatctgctt cggcggggtg
     1981 agggcgcgtt cacctccgcc tgggtgtccg accacctgat gaagaacgac catccggtcg
     2041 tcgaggcatg gacgacgatc gcctacctcg cggcagaagc gccgagctac cgcttcggca
     2101 acctggtgct gtcccagtcg tatcgaaacc cggcgttgct cgcgaagatg gcggcgacgc
     2161 tccagtacct caccggtggc cgcctgatcc tcggcatcgg cgcgggctgg caggccgatg
     2221 agtacgaggc ctacggctac gagtacccgt cggccggcgt gcgggtagag cagctcgccg
     2281 aggcgatcga cgtgctccga gcgatgtgga cgcagtcgcc ggcgacgtac gagggcaagc
     2341 actaccgggt tcgggacgcg tactgcgaac cgcggcccga ggcgccgatc ccgatcctgg
     2401 tcggcggccg tcgcccgaag ttcatgcgag tggccgccga gaaggccgac atctggcagt
     2461 gggatgggcc gatcgagcgc taccgggttc cgtatgacct gctcgtcgcc aactgtgccg
     2521 gagtcggccg agacctggcg tctgtcacgc tgtcgacgtt cggggaggtc tactttcccg
     2581 aggatcccgc tgacttccca cgcgagccgc tcctcgcgtt cacggatgcg gctcaggatc
     2641 catcgggcgc gtacgcaggc gaggccgatt gggtccttgg ccccacgccg gccgatgccg
     2701 ttgcggcatt gcggccgctg gtcgatctcg gtgtcggcca ggtcacgatc tacttctggg
     2761 acatgcgatc gctggacctg ttcatccgcg aggtcatccc ggcattccga tgatggggcc
     2821 tgtcagctgc agttggcgtc gatccacttg ctgacatcac cgagcgcctt gatgcgctcc
     2881 ggggactgcc cctgggtgag gatctctggt gccgggttgc ccgcaaggat ggactgcccg
     2941 acctcgtcga ccacgtcggc gagcaggcct gcagcaacct tgagatccgc cggcgcgtac
     3001 gttcgcatgt ccgttgcggc agcccggtac ccggcgccct ctgcctccgg ggtgtcctcc
     3061 tgggtggccg accggtactc cttgaatgcc gaacagaaat cggttgcgcc accgccaggg
     3121 gctggcgggg ctgtcgttgc tcctgccggc gcagaggcgg gcgcggcgac tgtcgaggcg
     3181 ggcgcgcctg ccgtcgaaac cggcactggg gtgggtgcat tggtcgccgt gggtgcgccc
     3241 gcgcacccgg cgaacatcgc gatggcaagc accgctagtg cacgacctct tctcgacatg
     3301 ggagcccctc ggattcggca ttgctcgccg gttgaccgca gagccgtagg ccccgcgggt
     3361 gcgtgatcca tggtattccg cgagatcggt cgatcgggct gatcctgacg ggcctcggcg
     3421 agaggtcaga gcagcaggaa gaccccgagg gcgatcagcc acagcgacca cgcgatgtag
     3481 gcgattggca cgatcgttcc agcgagcttc cagccgtcac gctcgttcgg gccgacgaac
     3541 tcgagcgtcc cgatcaggag ggcgatgccg atcggcaggc caacgatgcc gagccacgca
     3601 ggcagcaccg tggtggtcag gatcgaagct gcgatcagca gcgtccacag gccggtaaag
     3661 aggtagccca ggtgctcgcc gatgccgacg ccgagcaggc gatggagcat cgcgaacgtc
     3721 acctcgacgc tctggcgggt cgggtcgccg gctgggcctt cgtcggccgc gatgtaccgg
     3781 cgagcgagct ccggcacggc gaacggccag cgcaccaggc caagcccttg cacgagcgcc
     3841 gccgcggcgc cgacgacaat ggaaagcccg gtgagcaccg aatcggcgcc gatggcgagg
     3901 gccacgatga ccgcgagcgg cagcatcgcc agcgcgctca cgagcagcgc ctcccagcgc
     3961 aggatcaggc ccgcgccgcc cgccgagaac cggcggagga tctcgtccgg ctcgcgccgc
     4021 aggatgtccg gatagtcgaa ggagcgggcg agctcgaaga aggcgacgtt gaacgcgatc
     4081 gggacgagga tcaggaggat ggcggcggcg agctcgacgc tcacgactcc gcggcctcct
     4141 cagcggccgg cgagcgatgt gatcgcgggc gcgatcacat cgacccacag cgcgtactgc
     4201 gcgccactcg gatggagtcc gtccgaagcg acgagcgatc ggtccgtcgc cgcgccgagc
     4261 gagatgtcgt agatgtcggc gacggcgatt ccgcgcgcgg tcgcgagctc cgtgttgatc
     4321 gcgttgtagg cgcgaatctt ggctgactgg gcggtcgggt cgccgtagtc ggcgccggcc
     4381 ggggtcacgg tgtagtccgg cgtcgtgacg accaggacgt ggtcggcgcc gacctgtgcc
     4441 gccagggcgt cgaggatcac gcggacgttt gctcgatagg tggcctcggg cacgccctgg
     4501 accacgtcgt tgacaccgat caggagcgtg acgagatccg ggtcgagggt ggcgatctgc
     4561 ggcagctcga gctcgatgac gtcgctcgac gtaaagccgt ttaccccgag gttggcgacg
     4621 agctcgagga ccggcagccg gcgagcgagc tggtcgggcc agcgctcctg cgcagtcact
     4681 gatgtgccga tcgtgaacga gtcacccagg gcgacatagc ggaggcgagc cgtcccgccg
     4741 gcgccactgg atggcgcgcc ggcgctcggg cctgccgggc tcaacgacgc ttcgctcgtt
     4801 gtcgatggcg aaccgaccga gcaggctgcc agcaccgcca cgaggacggc gaggaggctc
     4861 gcgcgcgagc gcgccgtcgg atcacccggc gactcgtcat cccttgtcgt atggctgccc
     4921 gtccgctgcc ggcgcacgca cgcgtccaac gaggccggcg accacgatga tcgtcacgat
     4981 gtaggggacc atcagcagga actcggatgg cacgggcacg ttgaggatcg acagccgagc
     5041 ctgcagcgcg tccgcaaagc cgaagacgat cccggccagg aacgaaccaa tcggggtcca
     5101 tccgccgaag atcatcgcgg ccagcccgat gaacccacgg ccgccggtca tgttctggac
     5161 gaacgagccc gtggaaccga gggtgaaata cgcgccgcca acgccggcca cgaccccggc
     5221 gaagatgacg ttccggtagc gggtgagcag gacgttgatg cccaccgtgt cggccgcgcg
     5281 cgggtgctcg ccgacggcgc ggacgcgcag accccagcgt gtgtagaaca gcccgtagtg
     5341 gagcagcccg atgaggacga agagggcgta caggaagatg ttgccgttga agaacatcgg
     5401 accgaggacc gggatgtcgg acaggaccgg gatcgccagc gacgcaaact tctccggctc
     5461 gttcagctcg cgatgcgact gcaggacgcg ggcggcggcg aacgccgtga tccccgccgc
     5521 gaagatgttg atgaccgtgc ccgcgatgat ctggtccatc cggtagcgga tcgcgagcac
     5581 cgcgaggagg gcgcccaggc tccccccgac aagtgcggcg ccggccagcc cgatgatgtt
     5641 gttgccggtg gcgctgccca cgactgcgcc ggtgaatgcg ccgctcagga gcattccctc
     5701 gatcgcgatg ttgatcacgc ccgcgcgttc gcaaagcaac cccgacaacg cgccgaacgt
     5761 gatcggcagt gccttcacca cggtgagctg gaggatcccg atgaggctca tcgaccggcc
     5821 tgttgccgcc cacgtgagaa acccgaacac gaaggccacc acgacgaccc cgaggacgac
     5881 cgcccatctg ctcccgaatc cacggatgag ctgcatcgcg ccgagcgccg cgcagagcgc
     5941 cgcgatcacg tactgggtca agacggacgg cacgacgatg ttcgggacgt ccaacccgcc
     6001 ctggttcccg gccggcttga gctggaacgt gctgatcgca tcggcgtcga tgtccgacgt
     6061 gagcacgaag gcagcgatcg cggccgcgac gaggtacgtc acccccagga tccgctgccg
     6121 tcggacagcc gcacgatcca cgacgccctc gccgccgacg cgcaccgcgg tcatgagccc
     6181 caacctttgc tgaaggtctc cgtacccgtc gtctcgggca cgcggatgcg atagatcccc
     6241 cggacgagcg ccggcgccgc gacgaacatg atcacgagcg cctggatgac gaccaccagg
     6301 tcgatcggga cgcccgtgag cgattgcatc ggcgtcgctc cggcccgcag ggcgccgaac
     6361 aggagggctg cgaagatgat gcctctgggc cgcgatctgg cgatgagcgc gatcgcaatc
     6421 ccgtcgaacc cgaggcctgc agagatcccg gacgtgagcg atcgcgtcac gccgagcacg
     6481 atgctcgtcc cggcaagccc ggccaggccg ccgctgatcg ccatggcgag caccgtcgag
     6541 gacgcgatgc tcatgcccgc atatcgagca gcatgcgggt tgaagccgac cgctcgaaac
     6601 tcgaacccgc gggtcgatcg gaagagcagc caggagactg ccagcgctgc caccaggccg
     6661 atcacgaagc cccagttggc gcgcagccct tcgacgaggg ccggatacca cgaactcgcc
     6721 tcggtgacct tcgagatcgg gtcgctccgc ccttcgcgct ggtagccggg aaccttgagc
     6781 aggaactcga tcagccggta ggccgtgtag ttgagcatga tggtcacgat gacttcatgg
     6841 gccccggtcc tggccttcag gatcccgggg atgaagcccc agagcgcccc gccaagaaag
     6901 cccgcaccca ttgccagggg gaggtggatg aaccacggga cacccgtgac cgcgaagccg
     6961 acgaacgtcg cgaacgccgc accgacatac agctgaccgt ccgcgccgat gttgaacaga
     7021 ccgctctgga accccagggc gaccgcaagg ccggtaaaga tcagcggcgt cgctgcgagc
     7081 agcgtctcgc tgatgggcgc gaatgccttg gtgatctggc ccgcgtcgcc cgcggcgaac
     7141 gcgtcgatat aggtcccggg gttgccaacc gcgccggtca ggagggcgta atacgccttc
     7201 aggacgctgt cgagggccag gccgagcgcg gccgccgggt cctggctgag tcgctcgaag
     7261 aaggtcaggt cggacaggac gatgacgaag gcgccgatga tgagggcgct gaagatcgcc
     7321 aggaccggaa ccacgaggcc gcgcgcccaa cctgcgagcc gcgctcgcac gccgtttcgg
     7381 gcggctggct tgtcctgcgg gggtgccggc gacgacgggg caggccgact cggagtcggt
     7441 gtctgtcccg ggacatcagg aggcacgatc aaacccgccc tgcgtcacgc atcgggagac
     7501 accgcctcgg ttgcgagccc ggcggatccg gcctgggcgt cgctcaccag ccccgacccg
     7561 cccatcagca gcccgagcag ctgaggcgtt gcctgctcca cgtcgaggac gtcgacgatg
     7621 cggccctggt acatgaccgc aatccgatca gcgagggcca tcacctcgtc gagctccgtc
     7681 gacacgatca ggacggcggt gccttcgtcc cgcttctcga caatgcgccg atggatgtac
     7741 tcgatcgagc cgacatcgag tcctcgggtc ggctgtgcgg caaccaggag atggatcggt
     7801 cgcgagaact cccgggccac gatgaccttc tgctgattcc cgccggacag gttgctgatg
     7861 tcgttgaaaa cggatgtggt ccggacgtcg aattccttga ccagcttgtg cgcggacgtc
     7921 gcgattgcgt cgcgatcgag gcgcaggccg tccgagaatg gcgcgttgta gtaggtgttg
     7981 aggaccaggt tctcggcgat cgagaaacca ggcacgcaac cgtcctcgag ccggtcttcg
     8041 ggaatgtagg ccacgcctgc ctcgagcacg gcgcgcggct cgagcttcac gaccttgccc
     8101 tcgtacgtga tcgtgcccgc gaccggctcg cgcaggcccg tgatcgcctc gaccagctcg
     8161 gtctggccgt tgccctgcac gccggcgatc ccgacgatct cacccgcccg gacgctgaga
     8221 tcgagaccat tgacggccag gtggccccgg tcatccc
//
LOCUS       DATBSW010000327         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_207744, whole genome shotgun sequence.
ACCESSION   DATBSW010000327 DATBSW010000000
VERSION     DATBSW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207744"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..2372)
                     /locus_tag="VIF63_08930"
     CDS             complement(27..2372)
                     /locus_tag="VIF63_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8239546.1"
                     /translation="MADAALPPPATIDIRQRVKAQAGRLRRVKRIDRFAVGFITFGGM
                     FIIVAVLFIFVFILGESVPLFRPASGEARGAVTLAAVPPLEYVPGAVARLDAAAVAPA
                     ARAAATSPAPVTLSEPGPALVFGTDEYQRYFYAVLSDGRTAFWSNTGAFAKQLPPTSL
                     GRVPISSASRSLEHDFVALGTEDGRVSLQQVRFTPRYENQKLVDLDLAVRDQGLVEID
                     PQKRPIRQVAYAEIDGRKTLLAVAGNDTVFAFRTDDEGGEQRLELKTRDGETITRVRI
                     GRSDTAVAATDKGNLYHWELLPEARLTEVVHVSEETVTALEYVLGNITLIVGDAKGNL
                     SGWFRVREKEDDPDPRFVKAHSYPGQGAAIAAIGASTRDKSFVTAGIDGTLVMRHMTS
                     ERSVISFPATGAQVDAVMLTPKLDGIIAKRQDGQLARYEIKAPHADASLGALFGKVWY
                     EGYAKPEYVWQSTGGTDDFETKFSLVPLVFGTIKGTFYALLFAIPLAIMGALYTSQFM
                     HPKVKARVKPTVEIMAALPSVVVGFIAGLWLASRVEREIVPVLLMIVLLPLFGTLGML
                     VWSGVPRGMRDRLRPGMEMAVILPLLVLGAAIALALGPWFEGAFFSGDFRLWLTSATN
                     LVYDQRNCLVVGLAMGFAVIPIIFTIAEDSFTSVPQHLTAASLALGASRWQTATRVVL
                     PTASPGIFSAVMIGFGRAVGETMIVLMATGNTPVLDWSVFNGMRTLSANIAVEIPEAP
                     YGGTLYRTLFLAGLVLFLMTFFVNTLAEVVRQRLRERYRAI"
     gene            complement(2450..3409)
                     /locus_tag="VIF63_08935"
     CDS             complement(2450..3409)
                     /locus_tag="VIF63_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8239547.1"
                     /translation="MKRLVTLAALVALVAPALAQQVQVDSGLAPYQKTSGVSGNINSV
                     GSDTMNNMMTLWAEAFLKLYPNVKVQVEGKGSSTAPPSLIAGTSQFGPMSRAMRATEI
                     DQFEAKYGYKPTQLRTSYDALAVYVNKDNPLEKLTLAQVDAVFSKTRRKGGKAVANWG
                     QLGLTGDWANRPISLYGRNSASGTYGFFKEHALGNGDYKDQVKEQPGSASVVQGVTED
                     RFGMGYSGIGYKTSGVKVVALANKDGEPFSAGSYEDVRSGKYPLNRFLYIYINRAPGK
                     PLDPLVKEFTKFIYSKEGQEVVVKDGYLPLPAEIAKQELAKLE"
BASE COUNT          567 a         1193 c         1216 g          586 t
ORIGIN      
        1 gctcgtgaac aggccgttca tgatctctag atcgcccggt accgctcgcg caggcgctgg
       61 cgcaccactt cggcgagcgt gttgacgaag aaggtcatca ggaacaggac caggccggcg
      121 aggaacagcg tccggtacag cgtgccgccg tacggcgcct cggggatctc caccgcgatg
      181 ttcgcggaca gcgtgcgcat gccgttgaac accgaccagt cgagcaccgg cgtgttgccg
      241 gtggccatca gcacgatcat ggtctcgccg acggcgcgcc cgaagccgat catgaccgct
      301 gagaagatcc ctgggctcgc ggtgggcagg accacgcgcg tcgcggtctg ccagcggctc
      361 gctccgagtg cgagcgaggc cgccgtcagg tgctgcggca cgctcgtgaa gctgtcctcg
      421 gcgatcgtga agatgatcgg gatcaccgcg aagcccatcg caagcccgac caccaggcag
      481 ttgcgctggt cgtacaccag gttcgtggcg ctcgtcagcc agagccggaa gtctcccgag
      541 aagaacgcac cttcgaacca gggaccgagc gccagcgcga tcgcggcgcc gagcaccagc
      601 agcggcagga tcaccgccat ctccatcccg gggcgcagcc ggtcgcgcat gccacgcggt
      661 acgcccgacc agaccagcat gcctagcgtg ccgaagagcg gcagcagcac gatcatcagc
      721 agcaccggca cgatctcgcg ctcgacgcgc gaggcgagcc agagccccgc gatgaagccg
      781 accacgacgc tcggcagcgc cgccatgatc tcgaccgtgg gcttcacgcg cgccttgacc
      841 ttcgggtgca tgaactgcga ggtatagagc gcgcccatga tcgcgagcgg gatcgcgaac
      901 aagagcgcgt agaacgtgcc cttgatcgtc ccgaacacga gcggcaccag gctgaacttg
      961 gtctcgaagt cgtcggtgcc gcccgtggac tgccagacgt actcgggctt ggcgtagccc
     1021 tcgtaccaga ccttgccgaa gagcgcgccc agcgaggcat ccgcgtgggg tgctttgatc
     1081 tcgtagcgcg cgagctggcc gtcctgccgc ttcgcgatga tcccgtccag cttgggcgtc
     1141 agcatcacgg cgtcgacctg agccccggtg gccggaaacg agatcactga gcgctccgag
     1201 gtcatgtggc gcatcacgag cgtgccgtcg atgccggccg tgacgaagct cttgtctcgt
     1261 gtcgaggcgc cgatcgccgc gatcgcggct ccctggccgg ggtaggagtg cgccttcacg
     1321 aagcgcgggt cggggtcgtc ctccttctcg cgcacccgga accagcccga gaggttgccc
     1381 ttggcgtcgc ccacgatcag cgtgatgttg ccgagcacgt actcgagcgc ggtgaccgtc
     1441 tcctcggaga cgtgaaccac ctcggtgagc cgcgcctccg gcagcagctc ccagtgatag
     1501 aggttgccct tgtcggtcgc cgcgacggcg gtgtcgctgc gtccgatccg cacgcgcgtg
     1561 atcgtctcgc cgtcccgggt cttgagctcc agccgctgct cgccgccctc gtcgtccgta
     1621 cggaacgcga acacggtgtc gtttcctgcc acggccagga gcgtcttgcg gccgtcgatc
     1681 tcggcatagg cgacctggcg gatcggccgc ttctgcgggt cgatctcgac gaggccctgg
     1741 tcgcggacgg cgagatcgag gtcgacgagc ttctgattct cgtagcgggg cgtgaagcgg
     1801 acctgctgca gcgagacgcg gccgtcctcg gtgccgagag ccacgaaatc gtgctcgaga
     1861 ctgcgcgagg ccgagctgat cgggaccctg cccagcgagg tcggcggcag ttgcttggcg
     1921 aaggctccgg tattcgacca gaacgctgtg cgcccgtccg acagcaccgc gtagaagtag
     1981 cgctggtact cgtccgtgcc gaagacgaga gcggggccgg gctcgctcag cgtgacgggc
     2041 gccggcgaag tggcggcggc ccgcgccgcg ggcgccacgg ccgcggcgtc gagtctcgcg
     2101 accgcgccgg gcacgtactc gagcggcggc accgcggcga gcgtcacggc tcccctggcc
     2161 tcgccgctcg cggggcggaa cagcgggacc gactcgccga ggatgaagac gaagatgaac
     2221 agcacggcga cgatgatgaa catcccgccg aaggtgatga acccgaccgc gaagcggtcg
     2281 atccgcttga cgcggcgcag ccggccggcc tgagccttga cgcgctgtcg gatgtcgatc
     2341 gtcgccggcg gcggcagtgc ggcgtcagcc atatccgaaa ggctccagtt ggcgagttca
     2401 cgggaacggg acgtgggggc gggcggcccg cccccacgcg gcggaacggc tactcgagct
     2461 tggccagctc ctgcttcgcg atctccgcgg gcagcggcag gtagccgtcc ttgacgacca
     2521 cctcctggcc ttccttggag tagatgaact tcgtgaactc cttgaccagc gggtcgagcg
     2581 gcttgcccgg cgccctgttg atgtagatgt agaggaagcg gttgagcggg tacttgccgc
     2641 tcctcacgtc ctcgtagctg ccggccgaga acggctcgcc gtccttgttg gcgagcgcaa
     2701 cgaccttcac gcccgacgtc ttgtagccga tgcccgagta gcccatgccg aagcggtcct
     2761 cggtgacgcc ctggaccacg gatgccgagc ccggctgctc cttcacctgg tccttgtagt
     2821 ccccgttgcc gagcgcgtgc tccttgaaga agccgtaggt gccgcttgcc gagttgcggc
     2881 cgtacaggct gatcggccgg ttggcccagt cgccggtgag gccgagctgg ccccagttcg
     2941 cgaccgcctt gccgcccttg cgccgcgtct tggagaagac cgcgtccact tgcgccagcg
     3001 tcagcttctc gagcgggttg tccttgttca cgtacacggc gagcgcgtcg taggacgtgc
     3061 gcagctgcgt gggcttgtag ccgtacttgg cctcgaactg gtcgatctcg gtcgcgcgca
     3121 tcgcacgcga catcgggccg aactgcgagg tgccggcgat cagcgagggg ggcgccgtgg
     3181 agctgccctt gccctcgacc tggaccttca cgttcgggta cagcttgagg aaggcctccg
     3241 cccacagcgt catcatgttg ttcatcgtgt cggagccgac actgttgata ttgccggaga
     3301 ctcccgaggt cttctggtac ggcgccaggc cggaatcgac ctggacctgc tgggcgagcg
     3361 cgggggccac cagggccacg agcgccgcaa gcgtcaccaa acgcttcatg aactctcctc
     3421 cattgccttt gcacagatcg tagaaggcgt gtgaaaactc cgggtttcgg gcgtgttaac
     3481 tcagcgttaa ctgtcactcc tcatcggcca ctggctgcat gcggagcggg attcactccc
     3541 gtgcagcatg cggggggtct gg
//
LOCUS       DATBSW010000328         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_171723, whole genome shotgun sequence.
ACCESSION   DATBSW010000328 DATBSW010000000
VERSION     DATBSW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171723"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1659)
                     /gene="purH"
                     /locus_tag="VIF63_08940"
     CDS             complement(112..1659)
                     /gene="purH"
                     /locus_tag="VIF63_08940"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8239548.1"
                     /translation="MRALLSVAGREGISVFARGLQALDVELFATDGTREHLAQDGIEA
                     KPVSELTGVGTTFSGQVKTFHHAIYAGILARRDQPEQLEELRKEGIEPIDIVVVNVQP
                     FGAEIGRALVGVDKAIDMIDVGGAALLVAAARNPAGVVAASSPDQYTKILGELRERGH
                     VSPELRAKLAAEAFGTVAAYHAEIAAYLHQLTGTNYPKRLAVVLEKVTDLRYGENPHQ
                     SAAFYRETTHRSGTIADATLVHGAFPSFNNLLDLDAAYRAANDFTAPTVVIAKHTEPV
                     GIASADELVEAYRKALETDPVASFGGIVGVNRELDGATAREIAANSYEAVIAPGYSSA
                     AIGILRKKAGIELMSIPPDPTEGMRDYGIANLDFKRVGGGLLVETLDTEELDRSSLQV
                     VTKRRPTLEELTDLLFAWRTVRHVSSNAIVLARSRASVGIGAGQASRQVSVEIALRRA
                     GDRAKLAVMASDAYFPFPDGIHMAAQAGVIAIIQPGGSIRDEMAIEVADRHHLAMVFT
                     GRRHFRH"
     gene            1725..2198
                     /locus_tag="VIF63_08945"
     CDS             1725..2198
                     /locus_tag="VIF63_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239549.1"
                     /translation="MAYSTVVAAHEDTLTELPWFYFFIGFDKRNFPDLFDPLWIGSGL
                     LLIALVVVYTLRTRALHRHKHYLDMWEWLFWSGLITYFLLVVGSIFRFDFIVILVILF
                     SGLFTMVYARFRKFPPLFEAYEHQLARQRYLARARSSRPEATIRTKGIRRRGKKR"
     gene            2235..2702
                     /locus_tag="VIF63_08950"
     CDS             2235..2702
                     /locus_tag="VIF63_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8239550.1"
                     /translation="MEIRRFGVGNRRPEGPIGSVGVEGQVIHSDARGVIAELAFRKGA
                     RIEPHDNPNTAWFICIEGGGFVLVGEEERRVAAGEAVLWPAGVTHGAWTVHGPMRAFV
                     VEFAGSDDAEMRGILEGVAKRLGPGEAHRAAKGEGRLRPREADPERDREGEPT"
     gene            2726..3127
                     /locus_tag="VIF63_08955"
     CDS             2726..3127
                     /locus_tag="VIF63_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239551.1"
                     /translation="MRRPATSLGLAIAASILAQGCGPSGGPALFGPSRCAGDVPYSFV
                     GWTTTATLHMQFERAGTVFAMVTEQPVVLTESDDGSGWVDEPGPRHQLWGRKVCIATE
                     PNSMEFGWVVGSTSVLFEDGVRVPGEDPVHP"
     gene            complement(3147..>3593)
                     /locus_tag="VIF63_08960"
     CDS             complement(3147..>3593)
                     /locus_tag="VIF63_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEY8239552.1"
                     /translation="AIRKCGTEIARKAPSTGGTLIAFALMGAAKVAVPSDAAALVRAA
                     AYLDAAATTIATRGQVAVGDRTMLDALRPAADAIRAAVDAGDVPGAAASAAASAADAG
                     ARSTAAMAAKVGRAGWLADRAAGSEDAGARLIAWVFEAATSTFRRP"
BASE COUNT          503 a         1230 c         1286 g          574 t
ORIGIN      
        1 cgccgacgcg atcgccgcgg cctcggtgac ccggatcatc tcgaaggcaa gaagctgctc
       61 caccgtggcg gcgctcgcgg gcatcagggc ctccttcgcg cgctcggtcg atcagtgccg
      121 gaagtgacgg cggccggtga agaccatcgc caggtggtgc cggtcggcga cctcgatcgc
      181 catctcgtcg cggatcgagc cgccgggctg gatgattgcg atgacgccgg cctgggcggc
      241 catgtggatg ccgtccggga acgggaaata cgcgtccgaa gccatgaccg cgagcttcgc
      301 ccggtcgccg gcacgtcgca gggcgatctc cacggagacc tggcggctgg cctggccggc
      361 tccgatgccc acgctcgcgc gactgcgggc cagcacgatc gcgttcgagc tgacgtggcg
      421 gacggtccgc caggcgaaca ggagatcggt cagctcctcg agcgtggggc gccgcttcgt
      481 cacgacctgg aggctgcttc gatcgagctc ctcggtgtcg agcgtctcca cgagcaggcc
      541 gccgccgacg cgcttgaagt cgaggttcgc gatgccgtag tcgcgcatgc cctcggtcgg
      601 gtcgggtggg atcgacatga gctcgatgcc ggccttcttt cgaaggattc caatcgcggc
      661 gctgctgtag ccgggcgcga tcacggcctc gtaggagttc gcagcgattt cgcgggcagt
      721 cgcgccgtcc agctcgcgat tcacgccgac gatgccgccg aatgaggcga cggggtcggt
      781 ttcgagggcc ttccggtagg cctcgaccag ctcgtcggcc gaggcgatgc cgaccggctc
      841 cgtgtgcttc gcgatcacga cggtcggcgc ggtgaagtcg ttcgcggcgc ggtacgcggc
      901 atcgaggtcg aggaggttgt tgaacgacgg gaacgcgccg tggacgaggg tcgcatcggc
      961 gatcgtcccc gagcggtgcg tcgtctcgcg ataaaaggct gcgctctggt gggggttctc
     1021 gccgtagcgc aggtcggtga ccttctcgag aaccaccgcg aggcgcttcg gatagttggt
     1081 gccggtgagc tgatggaggt aggcggcgat ctcggcgtgg tacgcggcga ccgtgccgaa
     1141 tgcctccgca gcgagcttgg cccgcagctc cgggctgacg tggccgcgct cacgcagctc
     1201 accgaggatc ttcgtgtact ggtccggact cgacgccgcg acgacgccgg ccgggttgcg
     1261 agccgcggcg accaggaggg ccgccccgcc gacgtcgatc atgtcgatcg ccttgtcgac
     1321 gccgacgagt gcccgcccga tctcggcgcc gaacggctgg acgttgacga cgacgatgtc
     1381 gatcggctcg atgccctcct tgcgcagctc ctcgagctgc tccggctggt cgcgccgcgc
     1441 caggattccc gcgtagatcg cgtggtggaa ggtcttgacc tggccgctga acgtcgtccc
     1501 gaccccggtc agctccgaga cgggcttcgc ctcgatgccg tcctgggcga ggtgctcgcg
     1561 ggtgccatcg gtcgcaaaga gctcgacatc caatgcctgg aggccgcggg cgaagaccga
     1621 gatgccctcg cgcccggcga ccgacagcag cgctcgcacc cgcagtcgtc ctccgtcgtg
     1681 ccgggccgtc gtttgcagtg ggttcgaagt atagccaagg gcccgtggca tactccaccg
     1741 tcgtcgcagc ccacgaggac acgttgaccg agctgccctg gttctacttc ttcatcggat
     1801 tcgacaagcg caactttccg gacctgttcg atccgctctg gatcggctcc gggctgctgc
     1861 tgatcgcgct ggtcgtggtg tacacgctgc gcacgcgcgc gctgcaccga cacaagcact
     1921 acctcgacat gtgggagtgg ctgttctgga gcgggctgat cacgtacttc ctgctcgtcg
     1981 tcgggtcgat cttccggttc gacttcatcg tcatcctggt gatcctgttc agcgggctgt
     2041 tcacgatggt ctacgcccga ttccggaagt ttccgccgct gttcgaggcg tacgagcacc
     2101 agctggctcg ccagcgctat ctcgcccgcg cccgcagctc ccggcccgag gcgacgatcc
     2161 ggacgaaggg catccgccgc cgcggcaaga agcgctgatc gcgggtcggt gatcccctcg
     2221 gcgagcgccg cgtgatggag atccgccggt tcggcgtcgg caaccgccgg cccgagggcc
     2281 cgatcggctc agtgggcgtc gagggccagg tcatccacag cgacgcacgc ggcgtgatcg
     2341 ccgagctcgc gtttcggaag ggcgcccgga tcgagcccca cgacaacccg aacacggcat
     2401 ggttcatctg catcgagggc ggcgggttcg tgctcgtcgg cgaggaggag cggcgggtcg
     2461 ccgcgggcga ggccgttctg tggccggccg gcgtgacgca cggggcgtgg accgtgcacg
     2521 gcccgatgcg cgccttcgtc gtggagttcg cgggctcgga cgacgccgag atgcgcggca
     2581 tcctcgaggg cgtcgccaag cggctcggcc cgggcgaggc gcaccgcgcg gcgaagggcg
     2641 agggccgcct gcggccgcgc gaggccgacc ccgaacgcga ccgcgagggc gaaccgacgt
     2701 agcagggaat cgcaggacga tggcaatgcg acgacccgcg acctccctcg gactcgcgat
     2761 cgccgcttcg atcctggcgc agggctgtgg tcccagtggc ggaccggcgc tgttcggacc
     2821 ttcgcgctgt gccggcgacg tgccctactc gttcgtcggc tggaccacga ccgccaccct
     2881 tcacatgcag ttcgagcgcg cgggcacggt ctttgcgatg gtcaccgagc agcccgtcgt
     2941 cctcaccgag tccgacgacg gcagtggctg ggtcgatgaa cccgggccca ggcaccagct
     3001 ctggggccgg aaggtgtgca tcgcgacgga gccgaattcg atggagttcg gctgggtcgt
     3061 gggcagcacc tccgtcctgt tcgaagacgg ggtccgagtg cctggtgagg atcccgtcca
     3121 cccgtaacgg accccgcgaa gcccggtcag ggcctccgaa aggtcgaggt cgcggcctcg
     3181 aacacccacg cgatcaggcg cgcgccggca tcctcgctgc cggcggcacg atcggcgagc
     3241 cagccggcgc ggcccacctt ggccgccatc gcggcagtcg atcgagcgcc tgcgtcggcc
     3301 gcactcgcgg ctgccgaagc ggcagccccc ggcacatcgc ccgcgtcgac cgccgcccga
     3361 atggcatccg ctgcgggccg cagggcgtcg agcatcgttc ggtcgccgac ggcgacctgg
     3421 ccgcgggtcg cgatggtcgt cgctgctgcg tcgagatacg cggcggctcg cacgagcgct
     3481 gcggcgtcgg acggcacggc caccttcgcg gcgcccatga gcgcgaacgc gatcagcgtc
     3541 ccgccggtcg atggcgcctt tcgggcgatc tccgtgccgc atttccggat cgc
//
LOCUS       DATBSW010000329         4702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_419545, whole genome shotgun sequence.
ACCESSION   DATBSW010000329 DATBSW010000000
VERSION     DATBSW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419545"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..627)
                     /locus_tag="VIF63_08965"
     CDS             complement(175..627)
                     /locus_tag="VIF63_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239553.1"
                     /translation="MLRLGREAGATRQFELPTRPKEPWRSADVVLGWPAWQLAIDAEC
                     WNTFGDLGDATRSSRRKLVELEQLAVATWGPDGQARLLWVVRDTKANRRLLARYPEIF
                     ATLFTGSSRAWVDALTTGSRPPEAPGLVWCDVSTGRIRAWYRRHVAGG"
     gene            646..885
                     /locus_tag="VIF63_08970"
     CDS             646..885
                     /locus_tag="VIF63_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239554.1"
                     /translation="MTGVGDLDLRVAIESNSEWYVEGDGNSVQGLEVDGSDAPFDPAD
                     HQPTDVRLLGEILLGPSTALAHLLDVAADPRLAFR"
     gene            1460..2794
                     /locus_tag="VIF63_08975"
     CDS             1460..2794
                     /locus_tag="VIF63_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8239555.1"
                     /translation="MTNEAGGRSLRARLAPSGTARERATSDRTTTNHRSKAPTDAAAN
                     PAANAAASAANPVAKTDANAAATPAPATAPTTAKTNGTGPHLDLVTPAATPAANPTTP
                     AAPPHTLIQDDPLAALLDEAQAAVSGGANALRDVRERYRTAYLEKVEHWETLRARNSA
                     DRPTNQQGTDEAMAGREVGVERSVLERIDLAAKSLENAWLFLAREDTSLVSDPSGPPS
                     AADAQMRIIEAQEQERTRLAREVHDGPAQALSNAIFQVEVVQRLLDRDERLARDELKQ
                     LREVLTRELKGVRAYLSQLRMPVLADLGLSGAIQEAADQIATALNVPVSVELDEGLDQ
                     LPETVEVVALRVIQEALQNARKHAQPSSIRIRMGLDTAGWTVEVRDDGKGFDADDPPV
                     SGRRHFGLQFMRERAELIGARFEVRSSPNLGTAVRMTIPPGAMGMPIPGEES"
     gene            2795..3529
                     /locus_tag="VIF63_08980"
     CDS             2795..3529
                     /locus_tag="VIF63_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8239556.1"
                     /translation="MTAETEGGAPARRGNVAADKIRILIVDDHALFRVGIANIFSRER
                     EFDVVGEAADGRAGLEMAGMVSPSIVLMDLSLPPPGGLETTQRIRREFPATAVIVMAA
                     DEDEESLFASIKAGAAAFIIKDISPEDLIHVVRRVSMGEFLINDKVFSKPAVATRVLK
                     EFRELAVYGQEAQPIFAPLSPREVEILDNIAQGMTNKQVAYALSISEQTVKNHMSSIL
                     RKLAVNDRTQAVVYAMRQGWIKMPED"
     gene            complement(3510..3896)
                     /locus_tag="VIF63_08985"
     CDS             complement(3510..3896)
                     /locus_tag="VIF63_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239557.1"
                     /translation="MQARRGLALALEQVDLGFHERGAPGDRDELGGELGQLLRREAGA
                     RREALGAERELFLGCRASRLVACRLGPGLIDEELDLLDLALEVGGLSREARGEIGGVR
                     DDREVRRRVRRVRNLGVRGDLSPPAS"
     gene            3840..>4702
                     /locus_tag="VIF63_08990"
     CDS             3840..>4702
                     /locus_tag="VIF63_08990"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8239558.1"
                     /translation="MEAQVDLLEGKRKSAARLHDSIAEHALLVRGLAGMPEGGKALAS
                     GKGKAGADDQQSVEDGGPMPPGVSRMILTAQEDLRREIARQMHDGPAQSLTNIVLQAQ
                     IVDRLMARDPDKAAAEVAALIAMVQRTLDATKTFIFDVRPMVLDDLGLVPTLRRATRD
                     RGRRFNVAVEFESVGVDRRLPVELESGLFRMLDDALAAHVAKRPDTLTMHLDWGERLV
                     FELTAGRKPEAVPDPQLPAEGAELPPALAEMVGERRKAYQDALEAARIASLSRLPDQA
                     WKEVSARARIL"
BASE COUNT          772 a         1744 c         1563 g          623 t
ORIGIN      
        1 ccgccggcgc gcctgctggt cgtcgacgcc gacaactccg gcatctggcc cgccgcgatc
       61 ctgagcggtg cccccggcag cgaatgcttc gtcccgatcg agctgcccaa cctcgccgcc
      121 taccccggcg ccctcgacga cgtcctcgcc ttccggatcc gctgcccctg atcgtcatcc
      181 gccagctaca tgccggcgat accaagcgcg aatccgaccg gtagagacat cgcaccagac
      241 caggcccggc gcctcgggcg gccgcgaacc tgtcgtcagg gcatcgaccc acgcgcgtga
      301 ggatccggtg aacagggtcg caaagatctc cgggtatcgg gcgaggagcc tccggttcgc
      361 cttcgtgtcg cggaccaccc acaacagccg cgcctgcccg tcgggacccc aagtcgcgac
      421 ggccagctgc tcgagctcga cgagcttcct tcgactcgat cgcgtcgcgt cgccgaggtc
      481 gccgaacgtg ttccagcact cggcgtcgat cgccagctgc cacgcgggcc agccgagcac
      541 gacgtccgcc gagcgccagg gctccttcgg gcgagtcggc agctcgaact gcctcgttgc
      601 gccggcttcg cgcccgaggc gcagcatcgc ctcctggacg gagagatgac cggcgtcggc
      661 gacctcgact tgcgggtcgc gatcgaatcg aacagcgagt ggtacgtcga gggcgatggc
      721 aattcggtcc agggtctcga ggtcgatggg tccgacgccc cgttcgatcc ggccgaccac
      781 cagccgaccg acgtccgcct gctcggcgag atcctgctgg gtccatcgac ggcgctcgcg
      841 catctgcttg atgtcgccgc cgatccgcgc cttgcgttcc gctgatcgcc gctcagcctc
      901 gaccgcgacc gccgaacgcc gtgtcgctgc gtgcttcgag ggcgccttgg ggctgctttg
      961 ccccgtggcg gccgaacgcg ctggggcggc cgaacgcgcc ggggtcgact tggggcagct
     1021 tgtcaatcga ttgctctcgc catgctcgcg acatgatcat ttctggcact tacccaccga
     1081 tttcgcgacc tgggcgtcgg cgctgagggt tcggctgatc gaagcagccg ctcgccgatc
     1141 gggaatgcat cgtgcttgcg gtcggaccaa ggcccgcgaa ggctccttga ggggcaatct
     1201 cgagcccaat tggccaacga accccggaaa atgatcgccc tgtgaacacg ccccggcgtt
     1261 tgggccacgg gcgggcgacc agccccgggg agatgggccg cggggctcta cgggaacgca
     1321 acttgcgcct gccgcccgtc tcgcgaggcc gccgcaaggg acgggcgcgg cggcgggggg
     1381 cggcggacgc gggcggtccg ggtgggttcg ggaccattgg gctagggagc acgaatagcc
     1441 ccttcgtata ctcgcgacga tgaccaacga ggcggggggc cggtcgctgc gtgcgcgact
     1501 ggcaccttcg gggacggccc gtgagcgcgc gacgagtgat cgcacgacga ccaaccacag
     1561 gtcgaaggcg cccaccgacg ccgccgcaaa ccccgccgca aacgccgccg cgagtgccgc
     1621 gaaccccgtc gcgaagacgg acgccaatgc cgccgcgaca cccgccccgg caaccgcccc
     1681 gacaaccgcc aaaaccaacg gcaccggccc gcacctcgac ctcgtgacgc ccgccgccac
     1741 gcccgccgcc aaccccacca cacccgccgc cccgccccac accctgatcc aggacgaccc
     1801 cttggcggcc ctcctcgacg aggcgcaggc ggcggtctcc ggcggcgcga atgccctccg
     1861 cgacgtgcgc gagcggtacc gaaccgccta cctcgagaag gtcgagcact gggagaccct
     1921 ccgcgcgcgc aactccgccg accggcccac gaaccagcag ggcaccgacg aagccatggc
     1981 cgggcgagaa gtgggcgtcg agcgctcggt cctcgagcgg atcgacctcg ctgcgaagag
     2041 cctcgagaac gcgtggttgt tcctcgctcg cgaggacacg agcctcgtct ccgacccgag
     2101 cggcccaccg tcggccgccg atgcccagat gcgcatcatc gaagcgcagg agcaggagcg
     2161 gaccaggctc gcccgagaag tccacgacgg ccccgcccag gccctgagca acgcgatctt
     2221 ccaggtcgag gtcgtccagc gcctgctcga ccgcgacgag cgcctcgccc gcgacgagct
     2281 caagcagctc cgcgaagtcc tgacccgcga gctcaagggc gtccgcgcgt acctgagcca
     2341 gctgcgcatg ccggtcctcg ccgacctcgg cctgagcggc gccatccagg aagccgccga
     2401 ccagatcgcg acagccctca acgtcccggt aagcgtcgag ctcgacgaag gcctcgatca
     2461 gctcccggaa acggtcgagg tggtcgcgct gcgggtcatc caggaagccc tccagaatgc
     2521 gcgcaagcac gcccagccat cgtcgatccg catccgcatg gggctcgaca ccgccggctg
     2581 gaccgtcgag gtccgcgatg acggcaaggg attcgacgcg gacgacccgc ccgtcagcgg
     2641 gcgccggcac ttcggcttgc aattcatgcg agaacgcgca gagttgatcg gtgcacgatt
     2701 cgaagtacga tcgagcccga acctcggcac agccgtgcgg atgaccattc cgcctggtgc
     2761 catgggcatg ccgatcccag gggaggaaag ctgaatgaca gcggagactg aaggcggcgc
     2821 gcccgcccgg cgcggaaacg tcgcggcgga caagatccgg attctcatcg tcgacgacca
     2881 cgccctgttc cgggtcggga tcgccaacat cttcagtcgc gagcgcgagt tcgatgtcgt
     2941 gggcgaggcg gccgacggcc gcgccggcct cgagatggcg ggcatggtct ccccgagcat
     3001 cgtgctcatg gacctgtcgc tgcccccgcc gggcggcctc gagaccaccc agcgcatccg
     3061 ccgcgagttc ccggccaccg ccgtcatcgt gatggccgcg gacgaggacg aggaatcgct
     3121 gttcgcctcg atcaaggccg gtgctgccgc gttcatcatc aaggacatca gccccgaaga
     3181 cttgatccac gtcgtgcggc gggtctcgat gggcgagttc ctcatcaacg acaaggtctt
     3241 ctccaagccg gccgtcgcga cgcgcgtgct caaggagttc cgcgagctgg ccgtgtacgg
     3301 ccaggaggcc cagccgatct tcgcgccgct gtcgccgcgc gaagtcgaga tcctcgacaa
     3361 catcgcccag ggcatgacca acaagcaggt cgcgtacgcc ctgtcgatct ccgagcagac
     3421 cgtcaagaac cacatgagct cgatcctccg gaagctcgcc gtgaacgacc ggacccaggc
     3481 cgtcgtctac gcgatgcgcc agggctggat caagatgccg gaggactgag gtcaccacga
     3541 acgccgaggt tgcggactcg gcggactcgc cgccggacct cacgatcgtc ccgaacgccg
     3601 ccgatctcgc cgcgcgcttc gcggctgagg ccgccgacct ccagggcgag gtcgaggaga
     3661 tcgagctcct catcaatcag gccaggaccg aggcgacacg ccacgaggcg cgacgcacgg
     3721 cagcccagga aaagctcgcg ctcggcgccg agcgcctcgc gtcgggcacc ggcttcacgc
     3781 cgaaggagct gaccgagctc gccacccagc tcgtcgcgat cgcccggcgc tccgcgctca
     3841 tggaagccca ggtcgacctg ctcgagggca agcgcaagtc cgcggcgcgc ctgcacgact
     3901 cgatcgccga gcacgccctg ctcgttcgcg ggctggcggg catgccggaa ggtggcaagg
     3961 ccctcgcgtc cggcaagggc aaggccggcg cggacgatca gcagagcgtc gaggatggcg
     4021 gcccgatgcc gcccggcgtg tcccggatga tcctgaccgc ccaggaggac ctgcggcgcg
     4081 agatcgcccg ccagatgcac gacgggccgg cgcagagcct gaccaacatc gtcctccagg
     4141 cccagatcgt cgatcggctg atggcccggg acccggacaa ggctgcggcc gaggtcgccg
     4201 cgctcatcgc gatggtccag cgcacgctcg atgccacgaa gacgttcatc ttcgacgtcc
     4261 ggccgatggt cctcgacgac ctcggcctgg tgcccacgct tcggcgcgcc acccgcgatc
     4321 gcgggcgacg cttcaacgtg gccgtcgagt tcgagtccgt cggcgtcgat cgccggctgc
     4381 cggtcgagct cgagagcggc ctgttccgga tgctcgacga cgccctcgcc gcgcacgtcg
     4441 cgaagcgccc cgacacgctg acgatgcatc tcgactgggg cgagcgcctc gtcttcgagc
     4501 tgaccgccgg ccgcaagccc gaggccgtgc ccgacccgca gctgcccgcc gagggcgccg
     4561 agctcccgcc ggccctggcc gagatggtcg gtgagcgccg gaaggcctac caggacgcgc
     4621 tcgaagcggc gcgcatcgcg agcctctcgc gcctgcccga ccaggcgtgg aaagaggtct
     4681 ccgcgcgggc ccggatcctc ag
//
LOCUS       DATBSW010000330         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_984377, whole genome shotgun sequence.
ACCESSION   DATBSW010000330 DATBSW010000000
VERSION     DATBSW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984377"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VIF63_08995"
     CDS             <1..549
                     /locus_tag="VIF63_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8239559.1"
                     /translation="HRQDVMQFAVPAGDARFGPDALPVWIDFEGSFYGFPSLDGVALK
                     ACPDWLGPIEGPDESARIVAEATVEASRTVLRRRFPAIADQRIVKTWTCFYEVTPDAH
                     FVIDRHPGLDNVWIAGGGTGHAFKHGPVIGEYLAALVSGDSATAGALGPPDDRFAIRP
                     RDPMRSFRTSGRNPLEEAASLP"
     gene            633..708
                     /locus_tag="VIF63_09000"
     tRNA            633..708
                     /locus_tag="VIF63_09000"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:666..668,aa:His,seq:gtg)
     gene            801..1760
                     /locus_tag="VIF63_09005"
     CDS             801..1760
                     /locus_tag="VIF63_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239560.1"
                     /translation="MHSTSNRSLTAGALLVAMVIAACGGGAAVTPVPTAAPGTTGPVV
                     TNAPIGTNVPTGPPHVEAAAEAPAGSAVSVTWTGPNGQGDFVTIVRSGTSAWTNEDYF
                     YTTEGSPANLTAPSIAGNYEIWYVSGADKSVLAGQEIKLTPFAGGLTAPDSVVGNTEF
                     DVAWVGPNGPGDYVTIVKAGTEKWTIEDYFYTTVGPTGQLLAPVAAGAYEILYVIGSD
                     STIQARRPITVTVVTATVSAPDEVPKGAQFEVSWTGPNGPGDFITIVVVGAPASAYNS
                     YFDTNGVPTGSLTAPDVAGNYEVRYVAGQSPQVVLGSRPIKVN"
     gene            complement(1819..>2390)
                     /locus_tag="VIF63_09010"
     CDS             complement(1819..>2390)
                     /locus_tag="VIF63_09010"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY8239561.1"
                     /translation="GHGMGAVEITAISGSSISLKTVDGWTRTITVDADTTYSKSGDTI
                     ALGDLAVGDDIAFKQTLEDDGSWTIDAIAVIPPRVGGEVTAVSGSTITVEGRDGTSAT
                     IKVTADTDYQVDGDDAALADVKVGMFLVAEGTENADGSLTATDVRAADPDSFPGRGGH
                     GFKFGPGPGGPWGDKPDATTAPSATGSAG"
BASE COUNT          358 a          840 c          829 g          363 t
ORIGIN      
        1 caccgccagg acgtgatgca gttcgcggtc ccggcaggcg acgcgcgctt cggcccggac
       61 gcgctgccgg tctggatcga cttcgagggc tcgttctacg gctttccctc gctcgacggc
      121 gtcgcgctga aggcctgccc cgactggctc ggcccgatcg aggggccgga cgaatcagcg
      181 cgcatcgtgg cagaggcgac ggtcgaggcg tcgcggacgg tcctccggcg gcgcttcccc
      241 gcgatcgccg accagcgcat cgtcaagaca tggacgtgct tctacgaggt cacgccggac
      301 gcccatttcg tcatcgaccg gcaccccggg ctcgacaacg tctggatcgc cggcgggggc
      361 acaggccacg ccttcaagca cggtccggtg atcggcgagt acctcgcggc gctcgtgagc
      421 ggcgactcgg ccaccgccgg cgccctcggt cctccggacg accgcttcgc gatcaggccg
      481 cgcgacccga tgcgcagctt ccgaacgagc ggccgaaacc cgctcgagga ggctgcaagc
      541 ctgccctagc cccctgcccc gggtccgggc tacactgcgg gtcgctcacg gcctcccggg
      601 tgctcaggcg ccggtcgaga cgtgttcccg aggtggcctt agctcaaccg gcagagcagc
      661 ggattgtggt tccgcaggtt atgggttcga cccccatagg ccaccccaac ctcctccgcc
      721 ggtgcggccg aaattggcgc aagtggatgg tgtacggcgc ggcatcgcct cctaccatcg
      781 cgcaaacggg aggagacctg atgcattcga catcaaatcg atcgctgacc gcgggggcac
      841 tgctggtcgc gatggtcatc gccgcgtgtg gcggcggcgc cgccgtgacg cccgtgccga
      901 cggcagcacc ggggaccacc ggcccggtgg tcacgaacgc gccgatcggg accaacgtcc
      961 cgaccgggcc cccgcacgtc gaggctgccg ctgaggcacc cgccggatcc gcggtgtcgg
     1021 tgacctggac gggcccgaac gggcagggcg acttcgtgac gatcgtcagg agcggcacgt
     1081 ccgcctggac gaacgaggac tacttctaca cgaccgaggg ctcgccggcc aacctgaccg
     1141 cgccgtcgat cgccgggaac tacgagatct ggtacgtgtc cggggccgac aagtcggtgc
     1201 tggccggcca ggagatcaag ctgacgccgt tcgcgggcgg gctgaccgcg cctgactccg
     1261 tcgtcggcaa cacggagttc gacgtcgcgt gggtcgggcc gaacgggcct ggcgactacg
     1321 tcacgatcgt gaaggccggt accgagaagt ggacgatcga ggactacttc tacacgaccg
     1381 ttggaccgac cgggcagctg ctggccccgg ttgccgccgg cgcctacgag atcctgtacg
     1441 tcatcgggtc ggactcgacg atccaggctc gccgcccgat caccgtgacg gtggtgaccg
     1501 ccacggtctc ggcgccggat gaggtcccga agggcgccca gttcgaggtg tcgtggaccg
     1561 ggccgaacgg gccgggcgac ttcatcacga tcgtcgtcgt cggcgcgccg gcgagcgcct
     1621 acaactccta cttcgacacg aacggcgtac cgaccggcag cctcaccgcg ccggacgtcg
     1681 cgggcaacta cgaggttcgc tacgtggcgg gccagtcgcc gcaggtcgtg ctcggcagtc
     1741 gcccgatcaa ggtcaactag cagcaacgca gaacggccgg gcgggttgga cccgtccggc
     1801 cgttcgattc gccaggcgtt agccggcgct gccagtggca ctcggcgccg tcgtggcgtc
     1861 gggcttgtcg ccccacggcc cgccggggcc ggggccgaac ttgaacccgt ggccaccgcg
     1921 gccggggaag ctgtcgggat cggcggcgcg cacgtcggtc gccgtgagcg agccgtccgc
     1981 gttctccgtg ccttcggcga cgaggaacat gccgaccttg acatcggcca gggccgcgtc
     2041 gtctccgtcg acctggtagt ccgtgtccgc ggtgaccttg atcgtcgcgc tcgtgccgtc
     2101 gcgcccctcg acggtgatcg tcgagccgct cacggccgtg acttcaccac cgacacgcgg
     2161 cgggatgacc gcgattgcat cgatcgtcca cgacccgtcg tcctcgagcg tctgcttgaa
     2221 cgcgatgtca tcgccgaccg caagatcgcc gagggcgatg gtgtcgccgc tcttcgaata
     2281 ggtggtgtcc gcatcgaccg tgatcgtgcg ggtccagccg tcgacggtct tcagggagat
     2341 gctcgagccg gagatcgcgg tgatctcgac agcacccatg ccgtgaccga
//
LOCUS       DATBSW010000331         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1089209, whole genome shotgun sequence.
ACCESSION   DATBSW010000331 DATBSW010000000
VERSION     DATBSW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089209"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VIF63_09015"
     CDS             <1..1160
                     /locus_tag="VIF63_09015"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY8239562.1"
                     /translation="ADHPDNAWRFGLLCRAALEHLRAAARPPDVIHIHDWHTVPALLL
                     RDGLLGRDPAIGRAAFVLTIHNLAYHGWTAAAQVTELGLGPGALPRGADGIDLLRQGI
                     ERAELVNTVSPGFAAEALTAEYGMGLDGVLRARGDRFFGIVNGLDTDLWDPATDAALP
                     AQYSRDDRSGKARCRAALLAELGLDAADPRPVLAMIGRLDRQKGFDLLAEATPALLAD
                     GFRLAVLGSGNPDVVAPVRALAAGPGGKGRIALVERFDRDLARRMYAGADGFLMPSRF
                     EPCGTGQMIALRYGTPPIVRSTGGLRDTVIDVDADPRASTGFAFEPATAAALVDACSR
                     FRARFDAGGPAWAALLDRGMAVDFDWRTSSAPAYLAAYVRAIALRRVGASG"
     gene            complement(1194..2090)
                     /locus_tag="VIF63_09020"
     CDS             complement(1194..2090)
                     /locus_tag="VIF63_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8239563.1"
                     /translation="MTSRPAPTDTRLTATAADLPPAVAAALADPPQGEPGSVDAGGTT
                     WPTLSWGDPGKPPVLLVHGVTSNAGIWWRIGPAIAAAGKFVVAVDMPGHGRTAAAPRS
                     HRFADTAAEVVAFIRAAGLDRPDLAVVGHSWGGMVTAHLAGAGLAPARLILLDPPYLT
                     LERLRALTESPTERDYATLPEAVDAVRQSNPGWTDGDVEAKARALTEFVPEFVLNVLL
                     KNGNWDGGMAALRGPAAVPTPTWLIRGEWATGGFIPDSRVPKMEAQLGAGHVITIAGG
                     PHSPQRTHPEATVVALLRALSG"
     gene            2168..2557
                     /locus_tag="VIF63_09025"
     CDS             2168..2557
                     /locus_tag="VIF63_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8239564.1"
                     /translation="MANQFVVQLKNIPGAMAILAEELAARGVDLRAIGGGGLGNSGHV
                     IMTTADDEKTKQVLDEGGYTYVEGESIIAEVDDKPGGMARVARALADAGVNIHGHLFL
                     GRWGDRAMFAFVVDRPDVAKPILERKG"
     gene            2809..>3472
                     /locus_tag="VIF63_09030"
     CDS             2809..>3472
                     /locus_tag="VIF63_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY8239565.1"
                     /translation="MSRVASRFLIVAMATLMATGWITATPQPAAAVSPNVVISQVYGA
                     GGNASAIYQNDYIELYNRGAVAQSLAGWSLQYASAIGTGNLGATTTTLTELPNVSLPP
                     GQHYLVREAVGAGCGGGSIPCGAPLPLPDLVDATPIAMAAGAGKVALVDQATSLGCNG
                     GSTPCAATQLGHLIDLVGYGTGASGANFFEGTGPAPTLSATTAALRGADGATDTDNNA
                     SDF"
BASE COUNT          490 a         1225 c         1248 g          509 t
ORIGIN      
        1 cggccgacca cccggacaac gcgtggcggt tcgggctcct gtgccgcgcc gcgctcgagc
       61 accttcgcgc cgccgcgcgg ccgccggacg tcattcacat ccacgactgg cacaccgtgc
      121 cggcgctgct cctccgcgac ggtctgctcg gccgggatcc ggcgatcggg cgcgcggcgt
      181 tcgtgctgac gatccataac ctcgcgtacc acggctggac tgcggcggcc caggtgactg
      241 agctgggcct tggcccgggc gcgctgccca ggggcgccga cgggatcgac ctcctccgcc
      301 aggggatcga gcgggcggag ctggtgaata cggtcagccc cggcttcgcc gcggaggcgc
      361 tgacagccga atacggcatg ggcctcgacg gcgtgcttcg ggcgcggggc gaccggttct
      421 tcgggatcgt gaacggactc gataccgatc tctgggatcc ggcgacggac gccgcgctgc
      481 cggctcagta ctcgcgcgac gatcggtcgg gcaaggcccg ttgccgtgcc gcgttgctcg
      541 ccgagctcgg gctcgacgcg gcggatccgc ggccggtcct ggcgatgatc ggccggctcg
      601 accgccagaa gggcttcgat ctcctggccg aggcgacgcc ggcgctgctc gcggacgggt
      661 tccggctggc cgtccttggc tcgggcaatc ccgatgtcgt ggcgccggtt cgagcgctcg
      721 cggcgggccc gggcgggaag ggcaggatcg cactggtcga gcggttcgac cgcgacctgg
      781 cgcgccgcat gtacgccggg gccgacggct tcctcatgcc atctcgattc gagccgtgcg
      841 gcaccggcca gatgattgcg ttgcgctacg gcacgccgcc gatcgttcgc tcgaccggtg
      901 ggctgcgcga caccgtgatc gatgtcgatg cggacccgcg cgccagcacg ggcttcgcgt
      961 tcgagccggc gaccgccgcg gcgctggtcg atgcctgctc gcggttccgt gcccgcttcg
     1021 acgcgggcgg cccggcttgg gcggcgctcc tcgatcgcgg catggccgtc gacttcgact
     1081 ggcggacctc gtcagcgccc gcctacctcg ccgcctacgt tcgagcgatc gccctccgac
     1141 gagttggcgc gtcgggatga gctcgccgtc aggacccgac gcttcggtgc cgactagccg
     1201 ctcagcgccc gcaacagcgc gacaaccgtg gcctccgggt gtgtccgctg cggggaatgt
     1261 gggcccccag cgatcgtgat gacgtggccg gcgccgagct gcgcctccat cttcgggacc
     1321 ctggagtcgg ggatgaaccc gcccgtcgcc cactcgccgc gaatgagcca ggtcggtgtc
     1381 ggcaccgcgg cggggccgcg cagggccgcc atgccgccgt cccagttgcc gttcttgagc
     1441 aggacattga ggacgaactc gggaacgaac tcggtcaggg ctcgagcctt cgcctcgacg
     1501 tcgccatcgg tccaacccgg gttcgattgg cgaacggcat cgacagcctc gggcagcgtc
     1561 gcgtagtcgc gctcagtcgg gctctcggtg agcgcgcgca ggcgctcgag ggtcaggtat
     1621 ggcggatcga gcaggatgag cctggcgggc gcgagtcccg cgccggccag atgcgcggtg
     1681 accatgccgc cccacgaatg gccgacgacg gcaaggtccg ggcgatcgag accggcggcg
     1741 cggatgaacg cgacgacctc ggcggccgtg tcggcgaagc gatgggagcg aggggccgcg
     1801 gccgtgcgac cgtgaccggg catgtccacc gcgaccacga acttcccggc cgcggcgatg
     1861 gccggcccga tccgccacca gatgcccgcg ttcgaggtca cgccatgcac gaggaggacc
     1921 ggtggcttgc ccgggtcgcc ccaggacagc gtgggccacg tcgtcccgcc cgcatcgacc
     1981 gaaccaggtt cgccctgcgg gggatctgcg agcgcggctg caacggccgg cggcaggtct
     2041 gccgcggtcg ccgtcagtcg ggtgtcggtc ggcgccggtc ggctcgtcat agcagcactc
     2101 tagccaccgc tgcccgcgct cgggctacga tcaacgctgc cggcgcggtc cggccgagga
     2161 ggagtcgatg gccaaccagt tcgtcgtgca gctcaagaac atcccgggcg cgatggcgat
     2221 cctcgccgag gagctcgcgg cccgcggcgt cgacctgcgt gccatcggtg gtggcgggct
     2281 cggcaacagc ggccacgtga tcatgacgac ggccgacgac gagaagacca agcaggtgct
     2341 cgacgagggc ggctacacgt acgtcgaggg cgagtcgatc atcgccgagg tcgacgacaa
     2401 gcccggcggc atggctcgcg tcgcgcgcgc gcttgccgac gccggcgtga acatccacgg
     2461 ccacctgttc ctcggtcggt ggggcgatcg ggcgatgttc gccttcgtcg tcgatcgtcc
     2521 cgacgtggcc aagcccatcc tcgaacggaa gggctagcag gctcgacgct cgtccggctc
     2581 cgcgccgcgc gaggcgcatc ggcgcaccac gaagttcacc ggatgttgcc gcccggaaac
     2641 tgacgatttg caatggctcc gtaacttccg ggatgacccg ttcggtccgg cgctgggtac
     2701 agtcggggaa ggccgggata cccaaatggc cgcttggcct gggatccgac cgtcacgtct
     2761 ttcgtgggca tcgggtctgg ggaggaccct cgcggggagg actggtccat gtcgcgcgtc
     2821 gcttcgcgct tcctcatcgt cgcgatggca acactcatgg ccaccggctg gatcacggcg
     2881 acgccgcaac cagcggctgc ggtcagcccg aacgtcgtca tcagccaggt ctacggcgca
     2941 ggcggcaacg cgagcgcgat ctaccagaac gactacatcg agctctacaa ccggggcgct
     3001 gtcgcccagt cgctcgcggg gtggtccctc caatacgcga gtgcgatagg caccggcaac
     3061 cttggtgcga cgaccacgac gctgacagag ctgcccaacg tgagcctccc gccgggccag
     3121 cactacctgg tccgggaagc cgtgggcgcc gggtgcggtg gtggatcgat tccgtgcggc
     3181 gcgccactgc cgctgccgga tctggtcgat gccacgccga tcgcgatggc ggcgggcgcc
     3241 gggaaggtcg ccctcgtcga ccaggcgacg agcctcggct gcaatggcgg ctcgacacca
     3301 tgcgctgcca cgcagctcgg tcacctcatc gacctcgtcg ggtatgggac gggcgcgagt
     3361 ggcgccaact tcttcgaagg cactggtccc gcgcccacgc tctcggccac caccgcggcc
     3421 cttcgaggcg cagacggcgc cacggacacc gacaacaacg ccagcgactt cg
//
LOCUS       DATBSW010000332         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_631528, whole genome shotgun sequence.
ACCESSION   DATBSW010000332 DATBSW010000000
VERSION     DATBSW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631528"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VIF63_09035"
     CDS             <1..439
                     /locus_tag="VIF63_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8239566.1"
                     /translation="RAPFWDEPIAGAVFRHGYTYSGHSGAAAAALANLDIIEKEGLVE
                     RVATLEPILDRSVRRLAANELVGEIRTVGLTAAVAIKPEILEKDPTTIDKVIAAALRH
                     GVASRVLRAHAVHISPPFVITEAQIDAMVDGIGNALEDVASAG"
     gene            436..960
                     /locus_tag="VIF63_09040"
     CDS             436..960
                     /locus_tag="VIF63_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8239567.1"
                     /translation="MIAIKLVVNGEPVELEVEPRRLLVDMLREDLDLTGAKVGCDTSS
                     CGTCTVHLDGRAVKSCTVLAVQADGSTVRTVEDLAPDSEHLHPLQAAFVEHHGLQCGF
                     CTPGMLMTALDLLARDPDPADGAIRHAIHGNLCRCTGYQSIVTSIQEGAAVMRGDAPR
                     TWTEQHGAPRPAAR"
     gene            957..1841
                     /locus_tag="VIF63_09045"
     CDS             957..1841
                     /locus_tag="VIF63_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8239568.1"
                     /translation="MIPAPFDYARAASLDEALELVAGADPSIRVLAGGQSLIPLMKLR
                     LARPERLVDLGRLPELRGVRVRPDGGITVGALTTWADLLADPQIRAYGVLADAIPDIG
                     DTQVRNLGTIGGSLAHADPASDIAAPALVLEVELVARSAARGERVIRATEFFLGPFAT
                     ALEPDEILTEVRLPGPLDNTGSAYRALPQQASGYPIAGVAVVLTRAMAAGEERGSATT
                     SFSRCAIAVTGVGDHPYRANEVERAVLDGTPWSEAIAVITAGQRIAADMHADRDYRAA
                     MAVVMARRAFEAALARIG"
     gene            2330..3271
                     /locus_tag="VIF63_09050"
     CDS             2330..3271
                     /locus_tag="VIF63_09050"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8239569.1"
                     /translation="MTTHSTSNDPTPVGGAADLGTIAGVQTAFRERGYITDGSLATAV
                     FLALELGRPLLLEGEAGVGKTELAKVLASSLGARLIRLQCYEGLDVNAAVYEWNYPRQ
                     MLEIRLMEARGAASEATAHDIFGPDFLIRRPLLQALESTDGIPPVLLIDEIDRADEEF
                     EAYLLEILSDFQVTVPEIGTIKAERPPRVILTSNRTREVHDALKRRCLYHWIDYPTEA
                     KEFEIVLARVPSAPETLARNVVSFVHRLRSVDLTKVPGIAETLDWAAALLSLGAVELT
                     PQVVDETLGVVLKYEEDIRHVRGARVRELLAESSAKG"
     gene            3292..>3699
                     /locus_tag="VIF63_09055"
     CDS             3292..>3699
                     /locus_tag="VIF63_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239570.1"
                     /translation="MTSGATRQVDGRRLFGRAVGFGRALRRSGLAVDLAAAIDFARSL
                     EIVDIGHRDQVRAAGEAVFVRRKDDIEPYHLAFNAYWRGRLDLGAESLLDDGWEELVP
                     DENGQPGQEPVMAGDERIDRGEDTSGVPMPGEHD"
BASE COUNT          542 a         1343 c         1268 g          546 t
ORIGIN      
        1 ccgcgcgccg ttctgggacg agccgatcgc cggtgcggtg ttccgccacg gctacacgta
       61 ctcgggccat tcgggcgccg ccgccgccgc gctggcgaac ctggacatca tcgagaagga
      121 agggctcgtc gagcgggtcg ccacgctcga gccgatcctc gatcggtccg tgcgtcggct
      181 cgctgcgaat gagctcgtgg gtgagattcg gacggtcggc ttgaccgccg ccgtcgcgat
      241 caagccggag atcctcgaga aggacccgac gacgatcgac aaggtcatcg cggcggccct
      301 gcggcacggc gttgccagcc gggtcctgcg cgcgcacgcg gtccacatct cgccgccgtt
      361 cgtgatcacc gaagcgcaga tcgacgcgat ggtcgatggc atcggcaacg cgctcgagga
      421 cgtcgcgtcg gccggatgat cgcgatcaag ctcgtcgtca acggcgagcc ggtggagctc
      481 gaggtcgagc cgcgccggct cctggtcgac atgctgcgcg aggacctcga cctgaccggc
      541 gccaaagtcg gctgcgacac gtcgtcgtgc ggcacgtgca cggtccacct cgacggtcgt
      601 gcggtgaagt cgtgcaccgt gctcgccgtc caggccgacg gctcgaccgt gcgcaccgtg
      661 gaggatctcg cgcccgactc cgagcacctc cacccgctcc aggcggcgtt cgtcgagcac
      721 cacgggctcc agtgcggctt ctgtacaccg ggcatgctca tgaccgccct cgatctcctc
      781 gcccgcgacc cggacccggc cgacggcgcg attcgccacg cgatccacgg caacctgtgc
      841 cgctgtacgg gctaccagtc gatcgtgacc tcgatccagg aaggcgccgc ggtcatgcgc
      901 ggcgacgcgc ctcgaacctg gaccgagcag cacggcgcgc cgcggcccgc cgcccgatga
      961 tccccgcccc gttcgactac gcccgcgcgg cgagcctcga cgaggccctc gagctcgtgg
     1021 ccggcgctga tccgtcgatc cgcgtcctcg ccggcggcca gagcctcatc ccgctcatga
     1081 agctgcggct cgcgcgcccg gagcggctcg tcgacctcgg gcgcctgcca gagctccgcg
     1141 gcgtccgcgt ccgaccggac ggcggcatca ccgtcggcgc cctcacgacg tgggccgacc
     1201 tgctcgccga cccgcaaatc cgcgcctacg gcgtgctcgc cgacgccatc cccgacatcg
     1261 gcgacaccca ggtccgcaac ctcggcacga tcggcgggag tcttgcccac gccgacccgg
     1321 cgagcgacat cgcggcgccg gcgctcgtgc tcgaggtcga gctggtcgca cgctcggcgg
     1381 ctcgaggcga gcgcgtgatc cgcgccaccg agttcttcct ggggccgttc gccacggccc
     1441 tcgaacccga cgaaatcctc accgaggttc gactgccggg tccgctggac aacactggct
     1501 cggcctaccg ggcgctaccg cagcaggcgt cgggctaccc aatcgcaggc gtggcggtcg
     1561 tgctgacacg ggccatggcg gctggcgagg aacggggctc cgccacgacc tcgttctcgc
     1621 gttgtgcaat cgcggtcacc ggtgtcgggg accatccgta tcgcgcgaac gaggtcgagc
     1681 gcgccgtgct cgacggcacg ccctggtccg aagccatcgc cgtcatcacc gctgggcagc
     1741 gcattgccgc cgacatgcac gccgatcgcg actaccgagc cgcgatggcc gtcgtcatgg
     1801 cccgccgagc cttcgaggcc gcgctcgccc gtatcggctg acgccggccc ggcccgcacc
     1861 tcgcgagaac gccgctgtgc cacctttccg ctgggggcga gattcgagat agttcgaatg
     1921 gtgccgaacg gtcgcgccca gcccgctgat cgtcgcttca gtcgggcacc tcgaccgact
     1981 cgcctgaacg gggattggcg aatccatcgg gtccagaccg catcatgccg tggccgtccg
     2041 gccgtcgacc gggcagcttg ccggtgcctc tcccgccggc acgccgtgct tcgcgacaat
     2101 cgcgctgctg cctgtactcg atttcggact ccgcccagcg gccagcccgt cgaccgaccg
     2161 attggcggcc tgccttcgat agttcaccat cactcgaacc atcttgaatt tcggcctcgg
     2221 gggggggaaa cggggaaggg aagggggaac acgacgcgtc ggcgaacgct gaccccccgg
     2281 cgggccgagt gtcggcggat gctgtccgtt cgcgtagact cggcgctcca tgacaactca
     2341 ttcgacatcg aacgacccga cgcccgtcgg aggcgctgca gacctcggca cgatcgccgg
     2401 cgtgcagacc gcgttccgcg aacgcggcta catcactgac gggtcactcg cgacggccgt
     2461 cttcctcgcg ctcgagctcg ggcggccgct gctgctcgaa ggcgaggccg gcgtcggcaa
     2521 gaccgagctg gcgaaggtgc tcgcgtcgag cctcggcgcg cggctgatcc ggctccagtg
     2581 ctacgagggc ctcgacgtca acgccgcggt ctacgaatgg aactacccgc gccagatgct
     2641 cgagatccgg ctgatggagg cgcgcggcgc ggcctccgag gcgacggcgc acgacatctt
     2701 cgggccggat ttcctgatcc gccgcccgct cctccaggcg ctggagtcga ccgacggcat
     2761 cccacccgtg ctcctgatcg atgagatcga ccgggcagac gaggagttcg aggcgtacct
     2821 gctcgagatc ctgtcggact tccaggtcac cgtgcccgag atcggcacga tcaaggccga
     2881 gcgaccgccg cgggtgatcc tcacctcgaa ccggacgcgc gaagtccacg acgcgctcaa
     2941 gcgccggtgc ctgtaccact ggatcgacta cccgaccgag gccaaggagt tcgagatcgt
     3001 gctcgctcgc gtgccgagcg cgcccgagac gcttgcgcgc aacgtggtgt cgttcgtcca
     3061 tcggctgcgc tcggtcgatc tcaccaaggt cccggggatc gcggaaacgc tcgactgggc
     3121 tgccgcgttg ctctcgctcg gggccgtcga gctgaccccg caggtcgtcg acgagacgct
     3181 cggcgtcgtc ctcaagtacg aggaggacat ccggcacgtc cgaggcgcgc gcgtgcggga
     3241 gctgctcgcg gagtcgtcgg cgaagggctg agcatgaccg gcgaggcccc gatgacaagt
     3301 ggcgccacgc gccaggtcga tgggcggcgc ctgttcgggc gcgcggtcgg gtttggccgg
     3361 gccctgcgtc gatccgggct ggcggtcgat ctcgcggcgg cgatcgactt tgcgcgttcg
     3421 ctcgagatcg tcgacatcgg ccatcgcgac caggtgcgtg ccgccggtga ggccgtgttc
     3481 gttcgccgca aggacgacat cgagccgtac caccttgcct tcaacgcgta ctggcgcggc
     3541 cgcctcgacc tcggcgcgga gtcgctgctc gacgacggct gggaggagct cgtgcccgac
     3601 gagaacggcc agcccggcca ggagccggtc atggccggcg acgagcggat cgaccggggt
     3661 gaggacacga gcggggtccc gatgccgggc gagcacgac
//
LOCUS       DATBSW010000333         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_878152, whole genome shotgun sequence.
ACCESSION   DATBSW010000333 DATBSW010000000
VERSION     DATBSW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878152"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VIF63_09060"
     CDS             complement(<1..290)
                     /locus_tag="VIF63_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239571.1"
                     /translation="MHLPVPLGHPGPDHLLSIADLGPDGIADVVEHALVLKGQAGPGP
                     ARISPLAGKQVALLFDKPSLRTRVSFEVGIRRLGGATTSLSATDVGLGSREP"
     gene            412..>1509
                     /locus_tag="VIF63_09065"
     CDS             412..>1509
                     /locus_tag="VIF63_09065"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY8239572.1"
                     /translation="MQKVVLAYSGGLDTSVAVAWLQERHDVEVITLTVDVGGGSLRPG
                     AASRALSAGATRAYTVDARRKFVEGFVWPALRAGAMYQGAYPLATALARPLIAQLLVD
                     VAHAEGADAVAHGCTGKGNDQVRFDVAVKALDRRLEVIAPMRVGMGMTRDQEIVYAEE
                     RGIEIPVTHASPYSVDVNLWGRSVETGVLEDPWQTPPPDAYDWTVAPSVAPAPVEITI
                     SFDGGVPVAIDGAPLDPVELVETLNGLAGSHGVGRIDHIEDRLVGIKSREVYEAPAAV
                     LLHAAHRAIEGLALSKDQLRFGGVVATEVAQATYDGLWFTRLQRDLRGYVAAQQRVVS
                     GDVRIRLDHGTAAVVGRRSPNSLYDRALATYD"
BASE COUNT          216 a          547 c          534 g          212 t
ORIGIN      
        1 atcggctcgc ggctgccgag gccaacgtcc gtcgcgctca gcgaggtggt cgcgccgccg
       61 agccggcgga tgccgacctc gaaggagaca cgcgtgcgca ggctcggctt gtcgaacaga
      121 agcgccacct gcttgccggc cagcggcgag atccgcgccg ggcccgggcc ggcctggccc
      181 ttgaggacga gggcatgctc gacgacgtcg gcgatgccat ccggcccgag gtcggcgatc
      241 gagagcaggt ggtccggccc ggggtggccg agggggacag ggaggtgcat gcgggcgatg
      301 gtagacaact ctgcatgact atgcaagact ggcgtatccg ccgcatgtta atgcaggacc
      361 gagagccgca acatgcgccg ggcccacctc ccacgccctg gagctgatcc attgcagaag
      421 gtcgtcctcg cctattccgg tggcctcgat acgtccgtcg cggtcgcctg gctgcaggag
      481 cgccacgacg tcgaggtcat cacgctcacg gtcgatgttg gcggcggctc gctccgtccc
      541 ggcgccgcga gccgggccct gagcgcaggc gcgacgcgcg cctatacggt cgatgcccgc
      601 cggaagttcg tggaaggctt cgtgtggccg gcgctccgtg ccggcgcgat gtaccagggc
      661 gcctacccgc tggcgaccgc cctcgcccga cccctgatcg cccagctgct cgtcgatgtc
      721 gcccacgccg agggcgccga cgcggtcgcc catggctgca ccggcaaggg caacgaccag
      781 gtccgcttcg acgtcgccgt gaaggccctc gaccggcgcc tcgaggtcat cgccccgatg
      841 cgggtcggca tgggtatgac ccgggaccag gagatcgtct acgcggagga gcgcgggatc
      901 gagatcccgg tgacccacgc gtcgccgtac tcggtcgacg tcaacctgtg gggccgctcc
      961 gtcgagaccg gcgtgctgga ggacccgtgg cagacgccgc cgcccgacgc ctatgactgg
     1021 accgtcgcgc catcggtcgc cccggcgccc gtcgagatca cgatcagctt cgacggcggc
     1081 gtcccggtcg ccatcgacgg cgcgcccctc gatccggtcg agctcgtcga gaccctcaac
     1141 gggctggccg gcagccatgg cgtcggccgc atcgaccaca tcgaggaccg cctcgtgggc
     1201 atcaagagcc gcgaggtgta cgaggcgccc gccgccgtgc tgctccacgc cgcgcatcgg
     1261 gcgatcgagg gcctcgccct ctccaaggac cagctgcggt tcggcggggt tgtcgcgacc
     1321 gaagtcgccc aggcgaccta cgacggcctc tggttcacgc ggctccagcg cgaccttcgg
     1381 ggctacgtcg ccgcccagca gcgcgtcgtg agtggcgatg tccggattcg gctggaccac
     1441 ggcaccgccg cggtcgtcgg gcggcgcagc ccgaactccc tctacgatcg ggccctcgcg
     1501 acgtacgac
//
LOCUS       DATBSW010000334         5760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_560710, whole genome shotgun sequence.
ACCESSION   DATBSW010000334 DATBSW010000000
VERSION     DATBSW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560710"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VIF63_09070"
     CDS             <1..290
                     /locus_tag="VIF63_09070"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8239573.1"
                     /translation="EILHLLADGPLEVSGIAAAIGATQPHASQQLSVLRTAGLVEADR
                     VGREIRYGLADRDVIVACDVMRGVLQRRLARLARLATDQPFRPTIELGRSI"
     gene            312..725
                     /locus_tag="VIF63_09075"
     CDS             312..725
                     /locus_tag="VIF63_09075"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY8239574.1"
                     /translation="MDEPLNLVLFSGTDDKLHAAAVLTAGAAALGKPVNLFLQYWALD
                     AFRRERIDRDHGLAPEAGREGRDHVDDLRRAGQVSWAEILRQAKDLGAVDIQACSLSM
                     DLLDIESTDLDPLVDGVEGVTSFYLNAGQGQVVFI"
     gene            748..984
                     /locus_tag="VIF63_09080"
     CDS             748..984
                     /locus_tag="VIF63_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8239575.1"
                     /translation="MSALDLIAEVDARGLSCPMPIVRTAQAVRDLPSGMLVEVLATDP
                     GSERDFAAWCRSTGHTLVEHTTDGPIHRFLIRRK"
     gene            993..1574
                     /locus_tag="VIF63_09085"
     CDS             993..1574
                     /locus_tag="VIF63_09085"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY8239576.1"
                     /translation="MPVAAKFDPITEAIADAAGVPEVFVDALAARDSEVKARELVIIC
                     YSGDLEKTWASLILASTAAASGVPTKMFLTFWGLQTFVKDEVRITGQNWMQKMLSVMQ
                     RPGISHRKLSKMNFMGMGPWMMDTLAKQYGVAKPKELLEVALALGVELMPCQMTMDMF
                     GLTRDDMIDGMGEPVGAATVIELLSNGAAPLFI"
     gene            1605..1856
                     /locus_tag="VIF63_09090"
     CDS             1605..1856
                     /locus_tag="VIF63_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8239577.1"
                     /translation="MTAVVEARPTIRTVDARGSFCPGPLMELIRAIRESAVGDVISVL
                     STDKGSRLDIPKWIDKAGHRLISLEAHDGYDAIVVEKAR"
     gene            1861..3018
                     /locus_tag="VIF63_09095"
     CDS             1861..3018
                     /locus_tag="VIF63_09095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEY8239578.1"
                     /translation="MDRIVVLGGGVGGTLTANLLGRRLAHAIKLGQADVTLVDAFGAH
                     IYQPGFMYIAMGGEREEKLARPERALLDANVCLLVDRAVRVDTSARTLVLESGAILPY
                     DYLVLATGSRIVPDEIEHFAQEAQHFYSAAGARRLRQALDEFKGGRVVVGIASMPYKC
                     PPAPLEVTFLIEAELRERGLREKSEVHFASPIGRAFTIESVSEMATPILEEKGIELHT
                     FFNVEAIDPDRKVVTSLEGEELPYDLLVLVPPHRGQQFLIDSDLAPAPGGWLPTDRHT
                     LRVAGQTNIWALGDATDLPLSKAGSTAHFEAPVVAEGIVAAVAGREPEGKHATYKGRV
                     MCFFEVGDGKGTLLQFDYEHPPKPPKPNALWHVGKIVFNKTYFHTVPRGRV"
     gene            3216..3767
                     /locus_tag="VIF63_09100"
     CDS             3216..3767
                     /locus_tag="VIF63_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8239579.1"
                     /translation="MDTALVDRAKRGDAEAFDALARLVGDRCMAIAVRILRDADLADD
                     AVQTALITAWRELRSLRDASRFEPWLHRILTYECYAEARRRTRRPIDIRVVPDRSDDA
                     RGPLGVDDRDQLERAFRRLTLEQRAVLVFHHYLDLPVVDVADRLGIPVGTVKSRLHHA
                     TRALRASLEADARVPSVSEERPA"
     gene            3764..5059
                     /locus_tag="VIF63_09105"
     CDS             3764..5059
                     /locus_tag="VIF63_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239580.1"
                     /translation="MTALPEPDRKIRAFLDKGTVELPDRSFDAVRAAIDRTPQRTFIG
                     PWRNRPMTGTSRLAAVAAVLVVAVIGIVVVAMPALGPAATPPPTGSTKPSSTPTPTGA
                     APSSAAFLPYVWPEFLEPGTYTTTMNWDLPFKVALTVPPGWQSRDIEVIKGQMSVSVQ
                     LVENTFVDPCARTLRDPVTGPSVADLAAAFETIKDIDSDPPRATWRSGFDGLALTYTP
                     ARACEGVAGLLWTAPEEDLLPAEPKGPPNWPMRPGTHDVEILDVGGTRLLIDATAGPN
                     PTEAASRELFGVLGSLRILLAPPAQSVGECELSLAQSGGAPLVPPYQVVFGTGMSGLL
                     GPVPVDEQGRPLVPGPPFAQVDIHGTFPARTGDPNDRPGIAAIAPADVANTGFVTSTI
                     VGGFQGSVLMDAPGTWWVRFVADAWGCLEQFPVGVLARP"
     gene            5105..>5760
                     /locus_tag="VIF63_09110"
     CDS             5105..>5760
                     /locus_tag="VIF63_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8239581.1"
                     /translation="MTTSRVFRRSLDPALPVVARAEGSTIWDTDGKAYIDAAGGAIVV
                     GVGHGRESVARVMAEQASRVAYAHGTAFTSEPLERYAAALAPHLPMDDPAIYPVAGGS
                     EAMESALKLVRSYHLARGETERWIVFARWGSYHGNSLGALDLSGRKPLRRPYEAWLGR
                     FRHVSAAYPYRGGETGANALADADELAAELDQAFRAAGPGTVAAFVAEPIVGATLAAA
                     V"
BASE COUNT          905 a         2057 c         1896 g          902 t
ORIGIN      
        1 tcgagatcct ccatctcctc gccgacgggc cgctcgaggt gagcggcatt gccgccgcga
       61 tcggggcgac ccagccacat gcgtcgcagc agctgtcggt ccttcgaacc gccggcctcg
      121 tggaggcgga ccgggtcggt cgggagatcc gatacggact cgccgatcgc gacgtgatcg
      181 ttgcgtgcga tgtcatgcgc ggcgtgcttc agcgccgcct cgcccgcctt gcgcgcctcg
      241 ccacagacca gccgtttcga ccgacgatcg agctcggtcg ctcgatctga ccgcccagaa
      301 ggagcaccac gatggatgaa ccgctcaacc tggtcctctt ctcgggcacg gacgacaagc
      361 tgcacgccgc cgccgtcctc accgccggcg ccgcggcgct cggcaagccg gtgaacctgt
      421 tcctgcagta ctgggccctc gacgcgttcc gtcgcgaacg catcgatcgc gaccatggcc
      481 ttgcccccga ggcgggtcgc gagggccgcg accacgtcga cgacctccgt cgcgccggac
      541 aggtcagctg ggccgagatc ttgcggcagg cgaaggacct tggcgcggtc gacatccagg
      601 cctgctcact gtccatggac ctgctcgaca tcgagtcgac agacctcgac ccgctggtcg
      661 acggtgtgga aggcgtgacg tcgttctacc tcaatgccgg gcaaggccag gtcgtcttca
      721 tctgaccgtt cgaaaggaga cacatcgatg agcgccctcg atctgatcgc cgaggttgac
      781 gcgcgcgggc tctcctgccc gatgcccatc gtccgcaccg cccaggccgt tcgggacctc
      841 ccgtcgggga tgctcgtcga agtcctcgct acggatcccg gctcggagcg cgacttcgca
      901 gcgtggtgcc ggtcgactgg acacacgctc gtcgaacaca ccacggacgg cccaatccat
      961 cgcttcctga tccgccgaaa gtgaggcccg agatgccagt cgctgccaag ttcgatccca
     1021 tcaccgaggc gattgccgac gccgccggcg tccccgaggt gttcgtcgat gcgctcgccg
     1081 cgcgagactc agaggtcaag gcgcgtgagc tcgtgatcat ctgctacagc ggtgacctcg
     1141 agaagacatg ggcgtcgctg atcctcgcct cgaccgccgc tgccagcggc gtgccgacca
     1201 agatgttcct cacgttctgg ggcctccaga ccttcgtcaa ggacgaagtc cggatcaccg
     1261 gccagaactg gatgcagaag atgctgtcgg tgatgcagcg ccccggcatc agccaccgga
     1321 agctgtccaa gatgaacttc atgggcatgg gcccatggat gatggacacc ctcgcgaagc
     1381 agtacggcgt cgccaagccc aaggagctcc tcgaggtcgc gctcgcgctc ggcgtcgagc
     1441 tcatgccctg ccagatgacc atggacatgt tcggcctcac ccgggacgac atgatcgatg
     1501 gcatgggcga acccgtcggt gccgcgaccg tcatcgaact gctctcgaac ggcgccgcgc
     1561 cgctcttcat ctgaccgcaa gaaccccagg acaggagccc gaccatgacc gcagtcgtcg
     1621 aagcccgacc aaccatccgc accgtcgatg cccgcggcag cttctgcccc ggcccgctca
     1681 tggagctcat ccgcgccatc cgggagtccg ccgtcggtga cgtcatcagc gtcctctcga
     1741 cggacaaggg ttcccggctc gacatcccga agtggatcga caaggccggc catcgcctga
     1801 tttccctcga agcgcacgac ggctacgacg cgatcgtggt cgagaaggca aggtgatctg
     1861 atggaccgca tcgtcgtgct cggcggtggc gtcggcggca cgctcacggc caacctcctg
     1921 ggccgtcgcc tcgcgcacgc catcaagctt ggccaggccg acgtgaccct cgtggatgcg
     1981 ttcggcgcgc atatctacca gccgggcttc atgtacatcg cgatgggcgg cgagcgcgag
     2041 gagaagctgg cgcgtcccga gcgcgccctc ctcgacgcca acgtctgcct gctcgtcgac
     2101 cgggcggtcc gcgtggacac gtccgcgcgg acgctggtcc tcgagagcgg cgcgatcctg
     2161 ccctatgact acctcgtcct cgccacgggc tcgagaatcg tccccgacga gatcgagcac
     2221 ttcgcacagg aggcccagca cttctacagc gccgccggcg cacgccgcct gcggcaggcg
     2281 ctcgacgagt tcaagggcgg ccgcgtggtc gtcggcatcg cgtcgatgcc ctacaagtgc
     2341 ccgcccgcgc cgctcgaggt cacgttcctg atcgaggcgg aattgcgtga gcgcggcctc
     2401 cgcgagaaga gcgaagtcca cttcgcatca ccgatcggac gtgcattcac gatcgagtcc
     2461 gtctcggaga tggccacgcc gatcctcgag gagaagggca tcgagctgca caccttcttc
     2521 aacgtcgagg cgatcgatcc cgaccggaag gtcgtcacga gcctggaggg cgaggagctg
     2581 ccctacgacc tgctcgtcct ggtgccaccg catcgcggac agcagttcct catcgactcc
     2641 gaccttgcgc ccgcgcccgg cggctggttg ccgacggacc gccacaccct gcgcgttgcc
     2701 ggccagacga acatctgggc actcggcgat gcgacggacc tgcccctctc gaaggccggg
     2761 tcgacggcgc acttcgaggc gcccgtggtc gccgagggca tcgtcgccgc ggtggcaggg
     2821 cgagagcccg aaggcaagca cgcgacgtac aagggtcgcg tgatgtgctt cttcgaggtg
     2881 ggcgacggca agggcacgct gctccaattc gactacgagc acccaccgaa gccgcccaag
     2941 ccgaacgcgc tctggcacgt cggcaagatc gtgttcaaca agacgtactt ccatacggtc
     3001 ccgcggggtc gcgtctgatc ccatttgggg cgcgcctacg cgccgttccg tcccggcgca
     3061 gatgaagcgg cggcctgcca tgggccgccg cttcgtcgtg cacggctcga tcgttgccgg
     3121 cagccacgac gcagattgaa ccgagcggct ggggagcatc ctcttagctg tgaccgcccg
     3181 acccaagggc ggacgcgagg gagggatccc gcaccgtgga cacggcgctc gtggaccgcg
     3241 cgaaacgagg cgacgccgag gcgtttgacg ccctggcccg gctcgtcggc gaccggtgta
     3301 tggcgatcgc ggtccggatc ctgcgggacg ccgacctcgc ggacgatgcg gtgcaaaccg
     3361 cgctcatcac cgcatggcgg gagctgcgca gcctccgcga tgcgagccgc ttcgagccgt
     3421 ggctccatcg gatcctgact tacgagtgct acgccgaggc caggcgacgc acccgccggc
     3481 caatcgatat ccgggtggtg cctgatcgaa gtgacgacgc ccgcggcccg ctgggtgtcg
     3541 acgaccgtga ccagctcgag cgcgccttcc ggaggctcac gctcgagcag cgggccgtcc
     3601 tcgtgttcca tcactacctc gatctccccg tggtcgatgt ggcggaccga ctcggcattc
     3661 ccgtcgggac cgtgaagtcc cgactgcacc acgccacgcg tgcgctgcgc gccagcctcg
     3721 aggccgacgc gcgcgtgcca tctgtctccg aggagcgacc cgcatgaccg cgctgcccga
     3781 acccgaccgc aagatccgcg ccttcctcga caagggcacg gtcgagctgc cggaccgctc
     3841 attcgacgcg gtccgcgcgg caatcgaccg aaccccacaa cggaccttca tcggcccgtg
     3901 gaggaatcga ccaatgaccg gcacctctcg actggcggcg gtcgctgccg tcctcgtcgt
     3961 ggccgtgatc ggaatcgtgg tggtggcaat gcccgccctg ggcccggccg ccacaccacc
     4021 cccgaccggc tcaacaaagc cttcgtcgac gccgacgccc accggcgcgg ctccgtcctc
     4081 ggcagccttc ctgccctatg tctggcccga attcctggag cccgggacct acaccaccac
     4141 gatgaactgg gacctgccct tcaaggtggc gctgaccgtg ccccccggct ggcagagtcg
     4201 tgacatcgag gtcatcaagg gccagatgtc ggtgtccgtc cagctcgtcg agaacacctt
     4261 cgtcgatccg tgtgcccgga ccctgcgaga tccggtgacg ggtccatccg tcgcggatct
     4321 ggcagcggcg ttcgagacga tcaaggacat cgactccgat ccgccgcgag cgacgtggcg
     4381 atccggcttc gacgggctgg cgctcacgta tacgcccgcg agggcgtgcg agggcgtggc
     4441 cggtctcctc tggactgcgc ccgaagagga cctcctgccc gccgagccaa agggaccgcc
     4501 gaactggccg atgcggccag gaacgcacga cgtggagatc ctggacgtcg gcggcacgcg
     4561 cctcctcatc gacgcgacgg caggtcccaa cccgaccgag gcagcgagcc gggagctgtt
     4621 cggcgtgctc gggtcgctgc ggatcctgct ggcaccgcca gcgcaatccg tcggcgagtg
     4681 cgagctctcg ctggcgcaat caggtggagc gccgctcgtg ccgccgtacc aggtcgtctt
     4741 cggcaccggg atgtcggggc tccttggccc ggtccccgtc gacgaacagg gtcgcccgct
     4801 cgttccgggt ccgccatttg cacaggtcga catccacgga acgttcccgg caaggaccgg
     4861 tgatccgaac gaccggcccg gcattgccgc gatcgcaccg gccgacgttg cgaacacggg
     4921 attcgtaaca tccacgatcg tcggtggatt ccagggatcc gtgctgatgg acgcgcccgg
     4981 cacctggtgg gtgcgtttcg tagccgatgc ctggggctgc ctcgagcagt tcccggtggg
     5041 ggtattggcg cgcccctgat ccgatcggag ccgtgtttcg gccgggccta tcatggcccg
     5101 gccgatgacc acgtcccggg tatttcgccg ctcccttgac cctgcgctgc ccgtcgtcgc
     5161 gcgggccgag ggctcgacca tctgggacac cgacggcaag gcgtacatcg atgccgccgg
     5221 cggcgcaatc gttgttggtg tcggacacgg ccgcgagtcg gttgcccggg tcatggccga
     5281 gcaggcgtcg cgcgtcgcgt acgcgcacgg gacggcgttc acgagcgagc cgctcgagcg
     5341 ctatgccgcg gccctcgcgc cgcacctgcc gatggacgat cccgcgatct atccggtggc
     5401 cggcggctcc gaggcgatgg aatccgcgct gaagctcgtg cgctcgtacc acctcgcgcg
     5461 cggcgagacg gagcgctgga tcgtgttcgc tcggtggggg agctaccacg gcaattcgct
     5521 cggggcgctc gacctgtccg ggcgcaagcc gctgcggcgc ccctacgagg cgtggctcgg
     5581 caggttccgg cacgtctccg ccgcgtaccc gtatcggggc ggtgagaccg gcgccaatgc
     5641 cctcgccgat gccgacgagc tggcagccga actggaccag gcgttccggg ccgcgggacc
     5701 cggcacggtc gccgcgttcg tcgccgagcc gattgtcggc gcgacgctcg ccgccgccgt
//
LOCUS       DATBSW010000335         5710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1196155, whole genome shotgun sequence.
ACCESSION   DATBSW010000335 DATBSW010000000
VERSION     DATBSW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196155"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VIF63_09115"
     CDS             complement(<1..544)
                     /locus_tag="VIF63_09115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8239582.1"
                     /translation="MTEPIEPNPPVETAPRPIRRRGVGPFSLRQVTIAIVAMMGAAIL
                     GTLATVPLGKIEPGLPVPDPSAFLIGSPIPGLEVGKLAPALEGEGPDGHVQLTDLDGT
                     PIVLADLKGKAVWVNFWASWCPPCQYETPTLRSIDQKYRDRGLVIVAVQVQQTVEAGL
                     EYAERYGLGYTIGADVTGAIF"
     gene            complement(552..1382)
                     /locus_tag="VIF63_09120"
     CDS             complement(552..1382)
                     /locus_tag="VIF63_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8239583.1"
                     /translation="MDFTIAVAMGAGLLSFLSPCVLPLVPAYLGQLSAVAVVGREAGV
                     PSRWLAVRHSLAYVVGFGAVFTILGITATYLAGPLVDYLPILRQVGGALLIILGLNLA
                     GVIRIPALDRTWRPLEAGAAGSVASATGTMALASPGIAGASDAGTGARLGDRLGGRIV
                     SARGGWLASLGLGAIFAIGWTPCIGVILGGILTLAAGSGSAAQGAILLVAYTAGLGIP
                     FILIAAAYDRAPGLLGPLLRRGRLVSMIGGALVVAIGIAMLFDWLRLLPQYFPINTQI
                     "
     gene            complement(1382..1669)
                     /locus_tag="VIF63_09125"
     CDS             complement(1382..1669)
                     /locus_tag="VIF63_09125"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY8239584.1"
                     /translation="MSPQPDLVLYARPGCSLCDEAREAIGLVIGDRQARGLAVPNVLE
                     RNIEDDGELHRRLLERIPVVEIGNLRVELIVTVGKVRRLLNEALGDVATAG"
     gene            1727..1802
                     /locus_tag="VIF63_09130"
     tRNA            1727..1802
                     /locus_tag="VIF63_09130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1760..1762,aa:Arg,seq:cct)
     gene            2519..5098
                     /locus_tag="VIF63_09135"
     CDS             2519..5098
                     /locus_tag="VIF63_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8239585.1"
                     /translation="MSTHVISVAHSQPRPEERSGSAAAGAMVLGPAFIGGLALALAVV
                     VLLGWLSGNRALTTFIAGAVPMKANTALLLGLSGLGIILNTTGRVRGIVRVLATVVAA
                     VALATGLEYVTQFDFQIDNLLVLDTPTSGAPYPGRMAVMSIVAFGACSAALLLMGRTW
                     RGLHPSEFLALVAGAIGALGVLGYVYGATPLMQIGSDNRIALPASLGFVLLAGGVIAS
                     DADHGLTRLLRDPHLSGRLARRLVGISLVVLPISGVLAINLGRLESVGPASAIAVLVV
                     LQLVAFVVVGGWVIERTRRIERASASLVTERHQAQEAVEASEARLRTALDTMLDGTSI
                     VSSVRDDDNRIVDFRIDYANPAMARASGSGARDQVGKTLLELFPAHRTNGLFDAYVQV
                     VETGVAFTADDFRYVDPDAASGSLDLYLDLGAAKLGDGYVQSVRDVSDRYAARAAHDR
                     MTTQRDLIARTLSDLKAGSSTAETGEAICRQVVCLPGIVTANINSFTAEGPAISLAFV
                     RGDGVAVAARRIPTNRARAIRVLAEQGAWAEPWVRRPWHPYDGLFTQLGISAVGHAPI
                     VQDGRLIGILSVTATGSDALTDLRDALPAVVEFAGFAGALLGAAILDLSEVGGTRARI
                     AEIIRLASFHPVFQPVVDVVSGARVGYEALTRFANEQRPDTVFREARDAGLEVELELA
                     TLKAAILAAAGLPRGSWLSLNVSPALVTTEKRLAKLLRLADRPLVLEITEHVPVADYA
                     ALRAAIGQLRPAVRLAVDDAGSGTANLSHIVELRPAFVKLDIGLVRGIDTDLTRKAMV
                     FGLQHFAAESHSQTIAEGVETEAELAALRAIGVAYAQGYLLGHPTRVADWSAPGVERS
                     RIS"
     gene            5095..>5710
                     /locus_tag="VIF63_09140"
     CDS             5095..>5710
                     /locus_tag="VIF63_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239586.1"
                     /translation="MNLPGIVDRFLHPPLVTLLPAVTDRDGAGLDRTFGRFLVLCASA
                     VTVLLAAAVASVPPTERAAMAPVAALFAAMIGLSMWFYTRGFSRALFVLGGLALMTLG
                     IVVAATFPDGLDAAVILPLTGALLVLPVLRGRALLAMFVLAFGASMVGEVVAHLQATL
                     RQAPEPVSLPVSLAASAVMLAFTYGLVWWVSNQWLAASERSARTL"
BASE COUNT          835 a         2015 c         1933 g          927 t
ORIGIN      
        1 ggaagatcgc gccggtgacg tccgcgccga tcgtgtaccc gaggccatag cgctcggcgt
       61 actcgaggcc ggcttcgacc gtctgctgca cctggaccgc gacgatgacg agaccgcggt
      121 cgcggtattt ctggtcgatc gagcgcagcg tcggcgtctc gtactggcag ggcgggcacc
      181 agctcgccca gaagttgacc cagaccgcct tgcccttgag gtccgcgagg acgatcggcg
      241 tgccgtcgag gtcggtgagc tggacgtggc cgtccgggcc ctcgccctcg agcgcaggcg
      301 ccagcttgcc gacctcgagg cccgggatcg gcgagccgat gaggaacgct gatgggtccg
      361 ggacgggcag gccgggctcg atcttgccca gtggcacggt ggcgagcgtg ccgaggatcg
      421 cggcgcccat catcgcgacg atcgcgatcg tgacctggcg caggctgaac ggcccgacgc
      481 cgcggcggcg gatcggtcgc ggcgcggttt cgactggcgg gttcggttcg atcggttcgg
      541 tcatgtggtc gtcagatctg tgtgttgatc gggaagtact gcggcagcag gcgcagccag
      601 tcgaagagca tcgcgatacc gatcgccacc acgagcgcac cgccgatcat ggacaccagc
      661 cggccgcgcc ggagaagggg accgaggagc cccggcgccc ggtcgtaggc ggcggcgatg
      721 aggatgaacg ggatgccgag gccggcggtg tacgcgacga ggaggatcgc gccctgcgcg
      781 gccgagccgc tgccagcggc gagggtcagg atgccgccca ggatcacgcc gatgcacggc
      841 gtccagccga tcgcgaagat cgcgccgagg ccgagcgagg cgagccaacc accgcgtgcg
      901 ctcacgatcc gcccgcccag tcgatcgccg agtctcgctc cggtgccggc gtcgcttgct
      961 cccgcgatgc ccggcgatgc gagggccatc gtgcccgtgg cgctcgccac cgagcccgcc
     1021 gcacccgctt cgagcggccg ccacgtccga tcaagagccg ggatgcgaat cactcccgcg
     1081 aggttcaggc cgaggatgat cagaagggcg ccgccgacct gtcgaaggat cggaaggtag
     1141 tcgacgagcg ggccggccag atacgtcgcc gtgatgccga ggatcgtgaa caccgccccg
     1201 aacccgacga cgtaggcgag cgaatggcgc accgcgagcc agcgcgacgg cacacctgcc
     1261 tcccgcccaa cgaccgccac cgcggaaagc tgcccgaggt acgcaggcac gagcggcaaa
     1321 acgcacgggg agaggaagct cagcagaccc gcccccatgg ccaccgcgat cgtgaagtcc
     1381 atcagcccgc cgtcgccacg tcgcccagcg cctcgttcag cagtcgtcgc accttgccca
     1441 ccgtgacgat gagctcgacc cgcaggttgc cgatctcgac gaccggaatg cgttcaagca
     1501 ggcgtcgatg gagctcgccg tcatcctcga tgttgcgctc gaggacattc gggaccgcca
     1561 acccgcgcgc ctgccgatcg ccgatgacaa gtccgatcgc ctcgcgcgcc tcgtcgcaga
     1621 ggctgcaccc cgggcgcgca tacaggacga ggtcgggctg gggagacatc ggctcgatgc
     1681 tactcgacgc tcgccgaccc cgggcccggg gtacgatccg ggccgcgccc ccgtagctca
     1741 gtggacagag cgcagccgtc ctaaggctgg cgtcggcggt tcgagtccgt ccgggggcgc
     1801 cacagcaacg cagcctcgtc cgccgctggt cggtgcacgg caggctctcc ggctttcctc
     1861 gtcaccgacc gcgcccccga tcgtctgagt agggttggtg gtgcgatcaa gtgaccctca
     1921 acaggcgggg ggggggacat gaaccatcgt tggaacgtgg tcgcggcagc ggtcgtgctc
     1981 gtcttgctgc ttgtcggctg taggggcgtt ccgccacagg actcattgca ggactcgttg
     2041 cccgtcggaa cgtgcgtcct ggtgaacgat cgtggcacca cggtcgtgcc ttgcaccgag
     2101 ccgcattcgc acaaggtcat cgccatcgcc cggcgcgccg aagagtgtcc gcccgacacg
     2161 gacatgttct cccagcccgc agacccgcac gatggcctca cgaccacttg cttccagtcg
     2221 gacaccgcaa ccgactagtt ctatcgccca gcaacacgaa acgactcaga tcgcgaacca
     2281 cgagagacgg aacgggtccc tagccgtttg cgcccgtgcc ctggagtccg tccgggggcg
     2341 ccattcgtga cggcgcgagc gttggtcggt ccactcggtc cacgcagccg atcaggaggc
     2401 gaacgagtcc tccgggaagg acttccggat gggtgcgggg cggtgcttcc gactttaggg
     2461 tggcgaagcc ggtcgagtcc cgaccagggc ccgcgccacg ccacaccgat tggccttcat
     2521 gtccacgcac gtcatctccg tcgcgcacag ccagccgcgc cccgaggagc gcagcggaag
     2581 cgccgccgcg ggcgcaatgg tgcttggccc cgccttcatc ggtggactgg ccctcgccct
     2641 cgctgtggtc gtgctgctgg gatggctgtc tgggaaccgg gcattgacca ccttcatcgc
     2701 cggcgcggtt ccaatgaagg ccaacaccgc actgctcctc ggcctgtccg gcttgggcat
     2761 catcctcaac acgaccgggc gcgtccgcgg gatcgtccgg gtcctcgcga cggtcgtcgc
     2821 ggctgtggcg ctcgcgacgg gactcgaata cgtcacccag ttcgactttc agatcgacaa
     2881 cctgctcgtg ctggacactc cgacctccgg agcaccgtac ccgggccgca tggccgtcat
     2941 gtcgatcgtc gcattcggtg catgcagcgc agcgctcttg ctgatgggac ggacctggcg
     3001 cggactgcat ccgagcgaat tcctcgccct cgttgcaggc gccatcggtg cgctcggggt
     3061 cctggggtac gtgtatggcg ccactcccct catgcagatc gggtccgaca accggattgc
     3121 cctccccgca tcgctggggt tcgtcctgct cgccggcggg gtcatcgcct ccgacgccga
     3181 tcacggcctg acccgcctcc tccgcgatcc ccacctgtca gggcgtctcg cccgtcgcct
     3241 cgtcgggatc tccctcgtgg ttcttccgat ctcgggtgtc ctcgccatca accttggccg
     3301 cctcgagagc gtcggccccg caagcgcgat cgcggtcctc gtcgtccttc agctcgtggc
     3361 cttcgtcgtc gtcggcggct gggtgatcga gcgcacccgc cgcatcgagc gcgcgagtgc
     3421 gagccttgtg acggagcgcc accaggctca ggaggccgtg gaggcgagcg aggctcggct
     3481 ccgaactgcc ctcgatacga tgctggatgg gacaagcatc gtctcttccg ttcgtgacga
     3541 cgacaatcgc atcgtcgact tccggatcga ctacgccaac ccggccatgg ctcgggcaag
     3601 cggatcggga gccagggacc aggtcggcaa gaccttgttg gagctgttcc ccgctcatcg
     3661 cacgaacggc ctcttcgacg cctacgtcca ggttgtcgag accggcgttg ccttcacggc
     3721 cgacgacttc cgctacgtcg accccgatgc cgcgagcggt tcgctcgacc tgtatctgga
     3781 cctcggcgcc gcgaagctcg gcgatggcta cgtccagtcg gtccgcgacg tgtcggatcg
     3841 ctacgcggca agggctgcgc acgaccggat gacgacgcag cgggacctga tcgcccgaac
     3901 gctgtcggac ctgaaagccg gctcatcgac cgccgagacc ggtgaagcca tctgccggca
     3961 ggtcgtctgc ctccccggca tcgtcacggc caacatcaac tccttcacgg ccgaaggccc
     4021 tgcgatctcg ctggcattcg tccgcggaga cggggttgcg gtcgccgccc gacgcatccc
     4081 caccaatcgc gcgcgcgcca tccgggtgct tgccgagcag ggcgcctggg cggaaccctg
     4141 ggtgcgacgt ccctggcatc cgtacgacgg gctgttcacc cagctcggga tcagcgccgt
     4201 cggtcacgct ccgatcgtcc aggacggtcg actgatcggc atcctcagcg tgaccgcgac
     4261 cgggtccgac gccctgaccg acctgcgcga cgcgctgccc gccgtcgtcg agttcgccgg
     4321 gttcgcgggc gcgctccttg gggcggccat tctcgacctg agcgaggttg gcggcacccg
     4381 ggcgcggatc gcggagatca ttcgcctcgc cagcttccat cctgtcttcc agccggtcgt
     4441 cgacgtggtt tcgggcgcac gagtcggcta cgaggcgctg acccgcttcg ccaacgaaca
     4501 gcgtccggac accgtcttca gggaggcacg cgacgccggg ctcgaggtgg agctggagct
     4561 ggcgacgctg aaggcggcga tcctcgccgc ggccggattg ccccgcggct cgtggctcag
     4621 cctgaacgtc tcgccagcgc tcgtgacgac ggagaagcga ctcgccaagc tcctgcgact
     4681 tgcggatcgg ccccttgtcc tcgagatcac cgagcacgtt ccggtcgccg attacgccgc
     4741 gctccgggcg gcgatcgggc agctgcgacc agccgtccgc cttgccgttg acgacgccgg
     4801 ctcggggacg gcgaacctca gtcacatcgt cgagctccgg cccgcgttcg tgaagctcga
     4861 tatcggcctc gtccggggga tcgacaccga tctcacccgc aaggccatgg tcttcggcct
     4921 ccagcacttc gccgccgagt cgcatagcca gaccatcgct gaaggagtgg agaccgaggc
     4981 ggaattggcg gcgcttcggg cgatcggggt cgcctacgca cagggctacc tgctgggcca
     5041 cccgactcgg gtcgccgatt ggtcggcgcc gggtgtcgag aggtcgcgga tttcgtgaat
     5101 ctaccgggca tcgtcgaccg cttcctccac ccgccgctcg tgacgctgct gcctgccgtc
     5161 acggatcggg atggcgcggg gctggatcga acgttcggcc ggttcctggt cctctgcgcc
     5221 tccgctgtca ctgtgttgct tgcggcggct gtcgcgagcg tgcctcccac ggagcgagcc
     5281 gccatggccc cggtggccgc gctcttcgcg gcgatgatcg ggctgtcgat gtggttctac
     5341 acgcgcggct tttcgcgcgc gctgttcgtg ctgggcggac tggccctgat gacactcggg
     5401 atcgtcgttg cggcgacctt ccccgacggc ctcgatgccg ccgtgatcct gcccctcacg
     5461 ggcgccctcc tcgtgctgcc ggtcctgcgc ggacgggcgc tcctggcgat gttcgtcctg
     5521 gcgttcgggg cgagcatggt cggcgaggtg gtcgcccatc tgcaggccac cttgcgccag
     5581 gcgcccgaac cggtgagtct cccggtctcg ctcgcggcct cggccgtcat gctggcgttc
     5641 acctacgggc tcgtctggtg ggtgagcaac cagtggctcg ccgcgagcga gcgaagcgct
     5701 cggacgctgg
//
LOCUS       DATBSW010000336         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1196182, whole genome shotgun sequence.
ACCESSION   DATBSW010000336 DATBSW010000000
VERSION     DATBSW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196182"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VIF63_09145"
     CDS             <1..803
                     /locus_tag="VIF63_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8239587.1"
                     /translation="VLSRFGIEAFLPTLVSASMAVVDRARAAWAAGAPEGYSGATPLG
                     WHVEGPFISPKRAGAHDPATLQAPDARVVADWTPGSGIRMVTLAPELPGALEVVEALI
                     ANGVVVSAGHSNATFEQAAAGFDAGIRFITHLFNAMAPLDHREPGLAGAALADDRVTI
                     GIIPDGLHVHPSMVATVLRTVGSDRLAVVSDAIAALGMPPGTYRLADRDVACDGISAR
                     LPGGGLAGSVISLDQAVRNLATFAGIPIEDAKRAATTVPARLLGMDGA"
     gene            800..1846
                     /locus_tag="VIF63_09150"
     CDS             800..1846
                     /locus_tag="VIF63_09150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8239588.1"
                     /translation="MTLHDEILEQPEAARRQLASSTDALDALARRLRADPVSSVVVAA
                     RGTSDHAAIYAQYLLGVRNLLSVGLATPSVVSLYRAEPDVRRSLVIGISQSGASPDIV
                     GVVAAAARQGAPTLAITNDPASPLAAAADHVLDLAAGPELAVAATKTYSTSLLAIARL
                     SLALRPEPGDEAALAAVPQAMAAALEAEAEVVAITGELAGPGRSIDRCVVVGRGFEYA
                     TAREWALKLKELGRVFADPYSAADFLHGPIALVQAGIPVLVVAPEGVAAPGQIELLRD
                     LRERGVETVVASDVASTRDLGRWSIALPAGVPEWLRPAVSIVPAQLFAYHLTIARGLD
                     PDEPRYIQKVTRTT"
     gene            complement(1886..>2324)
                     /gene="ccsA"
                     /locus_tag="VIF63_09155"
     CDS             complement(1886..>2324)
                     /gene="ccsA"
                     /locus_tag="VIF63_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8239589.1"
                     /translation="QNPPLLTIHVGLAMLSYGVFATSFAAGVAYLVQGKGDRYSWLPS
                     HRVLDSVAYRAVIIGFPIFATMIILGSWWASIAWSRYWGWDPKETSALVTWLIYAIYL
                     HARNQRAWAGRPSALLLVIGFVMILVTLSGSLWFNGLHAYSGL"
BASE COUNT          322 a          867 c          807 g          328 t
ORIGIN      
        1 cggtgctcag ccgatttggc atcgaggcgt tcctgccgac gctcgtcagc gcgtcgatgg
       61 cggtcgtcga tcgcgctcgg gcagcgtggg cggcgggcgc gccggagggc tacagcggcg
      121 cgacgccact cggctggcac gtcgagggac cgttcatctc gccgaagcgc gccggcgctc
      181 acgatcccgc aaccctccag gcacccgacg cgcgcgtcgt cgccgactgg acgccaggct
      241 ccgggatccg gatggtcacg ctcgcccccg agctccctgg tgcgctcgag gtcgtcgagg
      301 ccctcatcgc gaacggcgtc gtcgtgtcgg caggccacag caacgccacc ttcgagcagg
      361 cagccgccgg cttcgacgcg gggattcgct tcatcaccca cctgttcaac gccatggccc
      421 cgctcgacca ccgcgagccc ggtctcgcgg gcgccgcgct cgccgacgac cgcgtgacga
      481 tcggcatcat tcctgacggc ctgcacgtgc atccttcgat ggtggcgacg gtgcttcgaa
      541 cggtcggatc cgacaggctg gcggtcgtga gcgacgcgat cgctgccctc ggcatgccgc
      601 ccggcaccta tcgactcgcc gatcgggacg tcgcctgcga cggcatctcg gcgcggctgc
      661 ccggcggcgg gctcgcgggc agcgtgatca gcctcgacca ggcggtccgg aacctcgcga
      721 cgtttgccgg gatcccgatc gaggacgcga agcgcgccgc gaccacggtg ccggcccggc
      781 tcctgggcat ggacggcgca tgaccctcca cgacgagatc ctggaacagc ccgaggccgc
      841 gcgccgccag cttgcctcct cgaccgacgc gctcgacgcg ctcgcccgcc gactgcgggc
      901 cgacccggtc tcgtccgtgg tcgtcgcggc ccgcggaacg tcggatcacg ctgcgatcta
      961 cgcccagtac ctgctcggcg tgcggaatct cctgtcggtc gggctggcga cgccgtccgt
     1021 cgtctcgctg taccgcgcgg agccggatgt ccggcgctcg ctcgtgatcg gcatcagcca
     1081 gtccggtgcg tcgcccgaca tcgtcggcgt cgtcgcggcc gccgctcgcc agggcgcgcc
     1141 gaccctcgcg atcacgaacg acccggcgag cccgctcgcg gcagccgccg accacgtgct
     1201 cgacctcgcc gccgggccgg agctcgccgt ggcggccacg aagacctact cgacgtcgct
     1261 cctggcgatt gcccgcctgt cccttgcgct tcgacccgag cccggcgacg aggcggcgct
     1321 ggcggccgtg ccgcaggcca tggcggcggc gctcgaggcc gaggcggagg tcgtcgccat
     1381 caccggcgaa ttggccggcc ctggccgatc gatcgaccgc tgcgtggtcg tcgggcgtgg
     1441 cttcgagtac gccacggcgc gcgagtgggc actgaagctc aaggagctgg gacgcgtgtt
     1501 cgcggacccc tactcggccg ccgacttcct gcacggtccg atcgcgctcg tccaggccgg
     1561 catcccggtg ctcgtcgtcg ccccggaggg ggtcgccgcg cccggccaga tcgagctgct
     1621 gcgcgacctt cgcgaacgcg gtgtcgagac cgtcgtcgcc tccgacgtcg cctcgacgcg
     1681 ggacctcggg cgctggtcga tcgcgctccc ggccggggtt cccgagtggc tccgaccagc
     1741 cgtctcgatc gtcccggccc agctcttcgc ctaccacctg acgatcgccc gcggcctcga
     1801 tcccgacgag ccccgctata tccagaaggt cacgcgaacg acgtagccgg ctgtcggcct
     1861 cgtgggacgg ccctgacccg gcgagctaca ggccgctgta cgcgtgcagg ccgttgaacc
     1921 agagcgagcc cgacagcgtg accaggatca tcacgaaccc gatgacgagc agcagcgccg
     1981 acgggcggcc ggcccaggcg cgctggttgc gggcgtggag gtagatcgcg tagatgagcc
     2041 acgtcacgag cgcggacgtt tccttcgggt cccagcccca gtagcgggac caagcgatcg
     2101 aggcccacca cgagccgagg atgatcatcg tggcgaagat cgggaagccg atgatcacgg
     2161 cgcgatacgc cacggaatcc agcacgcggt gcgagggcag ccacgaatac ctgtcgccct
     2221 tgccttgcac gaggtacgcg acgccggccg cgaacgacgt cgcgaagacg ccgtacgaga
     2281 gcatcgccag cccgacgtgg atcgtgagca gcggcgggtt ctgg
//
LOCUS       DATBSW010000337         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_702280, whole genome shotgun sequence.
ACCESSION   DATBSW010000337 DATBSW010000000
VERSION     DATBSW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702280"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VIF63_09160"
     CDS             <1..927
                     /locus_tag="VIF63_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239590.1"
                     /translation="DALLAVVVDRDTRSTVFAVRNMTLNAGYGIGAVLASLVVDLASP
                     SSFEALYAVDGATFLAFIPILALVRPRQAHLPAAELSRAMVDQRTSAPGYRAVLRDRV
                     FLRVWLLLAMLVAIGFAQTLSGFPAYATIVGGISAAGLSVAFAANTLTVVIAQFPILR
                     FLEGRRRTTGIVLACACWGAAWTITLATGNGGQSDGAVLWFAVAMVVFAIGETLLAPS
                     QAALINDLATDDLRGRYNGLYALAWTTGLAVGPAVAGAAISGGNGNALFGGLIVACAL
                     AAIYAARLARHLPADVNVVRIRSAKPGEAARA"
     gene            complement(890..2209)
                     /locus_tag="VIF63_09165"
     CDS             complement(890..2209)
                     /locus_tag="VIF63_09165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY8239591.1"
                     /translation="MTGPRTTATGEGGPTPWPLLLLPYAVILVAAALTDDFQLHSNQG
                     DIGLYLDKALALLSGLVPYRDYPLEYPPLALIPMVVPAVLWPFGPVTVELYKWLFAGW
                     EAVLMLALGYVLVRITRLGGDGDAWHDEPIGDVAGRLRTVAWRLFVLTIGAALAIAFR
                     YDLFPALLVMVAVWATLDRRPGVAGLSIGLGVVAKLFPLAIVPALVVPWLLPLDLRRL
                     TRFAVAAIGAVAVVMVPFLVLAGDDALAFLRYQSERGLQIESIGGGLAVLGGLIAGRP
                     VELTYGFSAVQVEGPFPIAWLAALPIATVVGFGLLGLVGWRRINADQRAGGIQPGTVV
                     ALAGAAVLVLLVTSKVFSIQYVVWIVPFAALLRGRQFWLAAALVALTMPIHPGLYADL
                     VKQLPLPILILNIRNGLLLVLLGMVVRDLATAAPAVRRGQLRRVSPT"
     gene            complement(2338..3117)
                     /locus_tag="VIF63_09170"
     CDS             complement(2338..3117)
                     /locus_tag="VIF63_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8239592.1"
                     /translation="MDDSTAPAFRALADPSRRLLLDGLFERDGQTLGELTARLPEMTR
                     FGVMKHLTVLEQAGLITTLREGREKRHFLNPVPIRLIHDRWISKYAAPIVGAMSALKG
                     QLEAPPMAVIPDQLDHVYSIFINAEPDRVWRAITDGDDTVQYFYGTRVVSDWAQGSHI
                     RYDNPDGTLAADGEVLSIDPPKRLEMSFLARWDPGLEAEGPVRQVWELDDVGGSTKLT
                     VTTYGLKAGSQMAEEFAGGIVYIVSGLKTLVEGGRTAVPAG"
     gene            complement(3175..>3662)
                     /locus_tag="VIF63_09175"
     CDS             complement(3175..>3662)
                     /locus_tag="VIF63_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8239593.1"
                     /translation="AVLLGGTGSGVELSAIGISLAGMAAVCQASYLVISRSGYSRLPA
                     DEASAVILAGAAVLIWFVALPLDIGGGRVAWLLSGTAWVAVLIAGLIGAAVAKVFVLR
                     AVRRVGGTRASVLMLSEPLAGVALAAVLLGQGLTPWQAMGGVGVLVGAVLAQRPAPGH
                     H"
BASE COUNT          623 a         1275 c         1253 g          511 t
ORIGIN      
        1 gatgcgctcc tcgcggtcgt cgtcgaccgc gacactcggt cgacggtgtt cgccgtccgc
       61 aacatgaccc tcaacgccgg atatgggatc ggagccgtgc tcgcttcgct ggtcgttgac
      121 ctcgcatcgc cgtcgagctt cgaggccctg tatgcggtcg acggggcaac attcctcgca
      181 ttcatcccga tccttgccct cgtccggccg cgacaggccc atttgccagc ggcggaattg
      241 agccgggcca tggtcgacca acggacgtcg gcaccgggct accgagccgt gttgcgcgac
      301 cgggtgttcc tacgggtgtg gttgctcctc gccatgctgg tggcgatcgg attcgcacag
      361 acgctttcgg gcttcccggc ctacgcgacc atcgtcggcg ggatcagcgc cgccggtctg
      421 agtgtcgcgt ttgcggccaa cacgttgacc gttgtcattg cccagttccc gatcctgcgc
      481 tttctggagg gccgacgtcg aaccacgggg atcgtgctcg cgtgtgcgtg ctggggcgcc
      541 gcctggacca tcacgctcgc caccggcaac ggtggccaat ccgatggtgc cgtgctctgg
      601 ttcgccgtcg cgatggtcgt gttcgcgatc ggcgagacgc tgctggcacc cagccaggcg
      661 gcactgatca acgacctcgc gaccgatgac ctccgcggcc gctacaacgg gctctatgca
      721 cttgcctgga cgaccggtct cgcggtcggt cctgcggtcg caggcgccgc catcagcggt
      781 ggaaatggga atgcgctctt cggcgggctc attgtcgcct gcgcgctcgc cgcgatctac
      841 gctgcgcgac ttgcgcgaca cctgccggcg gacgtcaacg tggtcaggat caggtcggcg
      901 aaacccggcg aagctgcccg cgcctgacag ccggcgccgc cgtggcgaga tcccggacca
      961 ccatgcccag cagcaccagc agcaggccat tgcggatgtt caggatcagg atcggcagcg
     1021 gcagctgctt gacgagatcg gcgtacaggc ccgggtggat cggcatcgtc agcgcaacga
     1081 gcgcggcggc gagccagaac tgccggcccc gcagcagcgc cgcgaacggc acgatccaca
     1141 cgacgtactg gatcgagaac accttgctcg tgacgagcag gacgaggact gccgcgccgg
     1201 cgagcgcgac aaccgtgccg ggctggatcc cgccggcccg ttggtccgcg ttgatccgtc
     1261 gccagccgac cagcccgagc aggccgaaac ccaccaccgt cgcgatgggc agcgcggcaa
     1321 gccacgcgat ggggaacggg ccttccacct ggactgcgct gaagccatag gtgagctcca
     1381 cggggcgacc ggcaatgagg ccgcccagga ccgcgaggcc gccgccgatc gattcgatct
     1441 ggagaccgcg ctcactctgg tagcgcagga acgccagcgc gtcgtcgccg gcaaggacca
     1501 ggaacggcac catcaccacc gcgacggcgc cgatcgcggc gacggcgaac cgcgtcagcc
     1561 ggcgaagatc cagcggcagc agccacggca ccacgagcgc gggcacgatg gcgagcggga
     1621 acagcttcgc aacgacgccg agcccgatcg acaagccggc gaccccgggc cgccgatcga
     1681 gcgtcgccca aaccgcgacc atcacgagca gggcagggaa gaggtcgtag cggaacgcga
     1741 tcgcgagcgc cgcgccgatg gtgaggacga acagccgcca cgcgaccgtc cgcaggcgac
     1801 ccgcgacgtc cccgatcggt tcgtcgtgcc aggcgtcgcc gtccccgccg agccgagtga
     1861 tccgcacgag cacgtacccg agcgccagca tgaggaccgc ctcccagccg gcgaacagcc
     1921 acttgtacag ctcgaccgtc accggcccga agggccacag caccgccggg acgaccatcg
     1981 ggatgagggc gagcggcggg tactcgagcg gatagtcgcg gtagggaacg aggccggaaa
     2041 gcagggcgag cgctttgtcg aggtacaggc cgatgtcgcc ctggttcgag tggagctgga
     2101 aatcgtccgt cagcgcggcc gcgacgagga tcacggcgta cgggaggagc agcaacggcc
     2161 acggcgttgg gcctccctcg cccgtagccg tcgtgcgagg tcccgtcacg agcggaaggc
     2221 taccagcggc acccgcacgg cccgcggtat tccgcgatca cgaaacgacc ctcgcgggag
     2281 tcccgcgagg gtcgtcattg atgcgaaggt cgcagccggg caagcgcccg gcggcgctca
     2341 gccggcgggg acagccgttc gaccgccctc gacgagggtc ttgaggcccg agacgatgta
     2401 cacgatgccg cccgcgaact cctcggccat ctgcgagccc gccttgagcc cgtaggtcgt
     2461 gaccgtgagc ttcgtcgagc cgcccacgtc gtcgagctcc cacacctgcc gaaccggacc
     2521 ctcggcttcg agcccgggat cccagcgcgc caggaacgac atctcgagcc gcttcggcgg
     2581 atcgatcgag aggacctcgc cgtcggccgc gagcgtcccg tcgggattgt catagcggat
     2641 gtgcgagccc tgtgcccagt cggacacgac gcgcgtgccg tagaagtact ggaccgtgtc
     2701 gtcgccgtcg gtgatcgccc gccagacccg gtcgggctcc gcgttgatga agatcgagta
     2761 gacgtgatcg agctggtcgg gaatgaccgc catcggtgga gcctccagtt gccctttgag
     2821 ggcgctcatg gcgccgacga tcggcgccgc gtacttgctg atccaacggt cgtggatgag
     2881 ccggatcggg acggggttga ggaagtgacg tttctcgcgc ccctctctca acgtcgtgat
     2941 cagcccggcc tgctccagga cggtcaggtg cttcatgacc ccgaagcggg tcatctccgg
     3001 caaccgagcc gtgagctcgc cgagcgtttg gccgtcgcgc tcgaagagcc catcgaggag
     3061 caggcgtcga ctcgggtcgg ccagcgcccg gaacgccgga gccgttgagt catccacgcc
     3121 ccggacaata ggtgaccttt cggtcacatg tcaagcggtc gtttgcctcg acaatcagtg
     3181 gtgtcccgga gccggccgct gggcaaggac ggccccgacg aggaccccga cgccgcccat
     3241 ggcctgccat ggagtcaggc cctggccgag gagcacagcc gcgagcgcca cgccggctag
     3301 gggctccgac agcatcagga cggacgccct cgtcccgccc acgcgacgaa cggcccggag
     3361 aacgaagacc ttggcgaccg ccgcgccaat gagccccgcg atcaggacgg cgacccatgc
     3421 ggtgcccgac aggagccatg cgacgcggcc gcccccgata tccaggggca gcgcgacgaa
     3481 ccagatcagg acggctgcgc cggcaaggat gacggcgctc gcttcgtcgg ctggcagccg
     3541 cgagtagccg gatcgggaga tgacgaggta gctcgcctgg cataccgcgg ccatgcccgc
     3601 aagcgagatt ccgatcgccg agagctcgac gccggatccg gttcctccca ggagcaccgc
     3661 ga
//
LOCUS       DATBSW010000338         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_667631, whole genome shotgun sequence.
ACCESSION   DATBSW010000338 DATBSW010000000
VERSION     DATBSW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667631"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..373)
                     /locus_tag="VIF63_09180"
     CDS             complement(2..373)
                     /locus_tag="VIF63_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8239594.1"
                     /translation="MTAALAARNETRGTTLADSVETAGSVWARFMGLMGRASLPVGHA
                     LWLPGDNGIHMFFMRFAIDAVFLAKPAADGSRAVVAAKRTVRPWIGMVPLVRGASGVL
                     ELPVGSIDASGTVAGDVVRLG"
     gene            complement(401..1321)
                     /locus_tag="VIF63_09185"
     CDS             complement(401..1321)
                     /locus_tag="VIF63_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8239595.1"
                     /translation="MEPILVAAAAAAAIIMIAFGVAGSREPDPLKARLSQLGQMTAKD
                     LQELELQAPFFDRTIKPLAARLSGAVQRITSSSFSERTEKRLAQAGNPGDMKVADWLG
                     IKAIGTGVGAGVLFILFGVLAGNITQGVLLGLVGAGIGYIGPEFWLGGRVRARQKGII
                     LQIPDALDLLTISVRAGLGFDAALGKCVEKMKGPLIDEFRRALAEVRVGKARREALRD
                     IVPRTEVQPLTNFIGAIIQAEQLGVSISKVLQVQSEQLRIERRQRAEEQAAQAPIKML
                     FPLVGCIFPSLFVVILGPALILIMQNLNTQ"
     gene            complement(1324..2337)
                     /locus_tag="VIF63_09190"
     CDS             complement(1324..2337)
                     /locus_tag="VIF63_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8239596.1"
                     /translation="MDFGTLTIAAAAAGAIILIALGISMSGSGGVTLERLERYASGDK
                     KGDAAGSGSGGVAELIQKSAALAQLNRVVEKRDFGANILRDLGAADLKLKPSEYIAIW
                     AGTTVGVPVVMFFLGFLVSALQNPVVLLIGLIIGFMAPRFWLNNRKNGRLNQFNKQLP
                     DTVTLIANALRAGSSFLQAIELVVRESRPPISIEFGRVIREVNLGLPFDLALENMVKR
                     VKSEDFELMATAIAIQHQVGGNLAEILDSIAFTIRERIRIKGEIRTLTAQQRLSGYVV
                     GFLPFGLAFFIFLAAPKFFDPMFQGPPDVIGIPLGVILLGVATLMMGAGFMFIRKIVD
                     IEV"
     gene            complement(2347..>2637)
                     /locus_tag="VIF63_09195"
     CDS             complement(2347..>2637)
                     /locus_tag="VIF63_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239597.1"
                     /translation="DLPVRAIREQISSAVDLIVHQSRLKDGTRKIVNITEVQGMEGDT
                     IVMQDVFVFEQTGVIDGKIQGRLKPTGIRPKFVELFESQGIHLPNNIFGFTF"
BASE COUNT          452 a          823 c          955 g          407 t
ORIGIN      
        1 cctagccgag gcggacgacg tcaccggcga ccgtgccgct tgcgtcgatc gacccgaccg
       61 gcagctccag gacgcccgag gcgccacgaa cgagcgggac catcccgatc cacggccgga
      121 cggtgcgctt ggccgccacg accgcgcgcg agccgtcagc ggccggcttg gcaaggaaga
      181 cggcgtcgat cgcgaagcgc atgaagaaca tgtggatgcc gttgtcgccg gggagccaga
      241 gcgcatggcc cacagggagg gacgctcggc ccatcaggcc catgaaccgg gcccagacgg
      301 aaccagccgt ctcgacggag tcagccagag ttgtgccgcg ggtttcgttc cgcgcagcga
      361 gcgcggcggt caccgagtcg cgctcaggcg ccgagctgcg tcactgggtg ttcaggttct
      421 gcatgatgag gatcagggct gggcccagga tgaccacgaa cagcgacggg aagatgcagc
      481 cgacgagcgg gaacagcatc ttgatcggtg cctgtgcggc ctgttcctcg gcgcgctgcc
      541 ggcgctcgat gcggagctgc tccgactgga cctggagcac cttcgagatc gacacgccga
      601 gctgctcggc ctggatgatc gcgccgatga agttggtgag cggctggacc tcggttcgcg
      661 ggacgatgtc gcgcagcgcc tcgcgccgcg ccttgccgac ccgcacttcc gcgagggcgc
      721 ggcggaactc gtcgatcaat ggccccttca tcttctcgac gcacttgccc agcgcggcgt
      781 cgaagccgag gccggcgcgg acggagatcg tgagcaggtc gagggcgtca gggatctgga
      841 ggatgatgcc cttctggcga gcgcggacgc ggccgccgag ccagaactcc gggccgatgt
      901 agccgatgcc cgcgccgacg aggccgagca gcacgccctg ggtgatgttg ccggcaagga
      961 cgccgaacag gatgaacagg acgccggcgc cgacgccggt gccgatcgcc ttgatgccga
     1021 gccagtcggc gaccttcatg tcgccggggt tgcccgcctg ggcgaggcgc ttctcggttc
     1081 gctcgctgaa cgagctcgag gtgattcgct ggactgcgcc cgacaggcgc gccgcgagcg
     1141 gcttgatcgt gcggtcgaag aacggcgcct ggagctccag ctcctggagg tctttggccg
     1201 tcatctggcc gagctgcgac agccgggcct tgaggggatc gggttcgcgt gagccggcga
     1261 cgccgaaggc gatcatgatg atcgcggcgg cggccgcagc ggcgacgagg attggttcca
     1321 tggttagacc tcgatgtcca cgatcttgcg gatgaacatg aagccggcgc ccatcatcag
     1381 ggtggcgacg cccaggagga tgaccccgag cgggatgccg atgacgtccg gcggaccctg
     1441 gaacatcggg tcaaagaact tgggcgcggc gaggaagatg aagaaggcga ggccgaacgg
     1501 caggaagccg acgacgtagc cggacaggcg ctgctgggcg gtcagggtgc gaatctcgcc
     1561 cttgatcctg atgcgctcac ggatcgtgaa cgcgatcgaa tcgaggatct cggcaaggtt
     1621 gccgccgacc tggtgctgga tcgcgatcgc ggtggccatg agctcgaagt cctccgactt
     1681 cacgcgcttg accatgttct cgagggcgag gtcgaacggc aggccgaggt tcacttcgcg
     1741 gatgacgcgg ccgaactcga tcgagatcgg gggccgcgac tcgcgaacga cgagctcgat
     1801 ggcctgcagg aaggacgagc cggcgcggag cgcgttcgcg atcagcgtga cggtgtccgg
     1861 cagctgcttg ttgaactggt tgaggcgtcc gttcttgcgg ttgttgagcc agaagcgcgg
     1921 cgccatgaag ccgatgatca ggccgatgag cagcacgacc gggttctgga gcgcagagac
     1981 caggaacccg aggaagaaca tcacgaccgg cacgccgacc gtggtgcccg cccagatcgc
     2041 gatgtactcg gacggcttga gcttgaggtc cgcggcaccg aggtcgcgca ggatgttcgc
     2101 gccgaagtcg cgcttctcga cgacccggtt gagctgcgcg agggcggccg acttctggat
     2161 cagctcggcg acgccgccgg agccagagcc cgcggcatcg cccttcttat cgccggacgc
     2221 gtagcgctcg aggcgctcga gcgtcacccc gcccgagccg gacatcgaga tgcccagcgc
     2281 gatcaggatg atggcgcccg cggcggccgc ggcgatggtg agcgtgccga agtccatgtc
     2341 gatcctttag aaggtgaagc cgaagatgtt gttcggcagg tggatgccct gcgactcgaa
     2401 cagctcgacg aacttcggcc ggatgccggt cggcttgagc cggccctgga tcttgccgtc
     2461 gatgacgccg gtctgctcga agacgaacac gtcctgcatg acgatcgtgt caccttccat
     2521 gccctggacc tcggtgatgt tcacgatctt gcgggtgccg tccttgagtc gcgactggtg
     2581 cacgatcagg tcgaccgcgg acgagatctg ctcgcggatg gcgcggaccg ggaggtc
//
LOCUS       DATBSW010000339         4727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_68273, whole genome shotgun sequence.
ACCESSION   DATBSW010000339 DATBSW010000000
VERSION     DATBSW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68273"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VIF63_09200"
     CDS             complement(<1..409)
                     /locus_tag="VIF63_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY8239598.1"
                     /translation="MDLRRLLAIARSRFPLLVAGILVGGGLAVLVSSLQPKVYEAKTT
                     LIVGQSLSGVATDFNQLLVSQRLSATYAAVATTRPILVAVIGELGLATTPEELIKRVR
                     SSAQLDSTLLTLSAQDSDRNRAAAIASALAEELI"
     gene            602..1138
                     /locus_tag="VIF63_09205"
     CDS             602..1138
                     /locus_tag="VIF63_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY8239599.1"
                     /translation="MASIAASLPWLLPGSIAALAVGTLASRALARWLGVRWITAWVVL
                     VSLGLILAGTLSPLATGGNAGPGVARTCDLSRTWPASFSDLAPGNDVAVNLVMFFPLG
                     CALGFAPLSRRTLLVLVGAIALPFAIEALQLEVAALGRACQGADVVDNLTGLAVGLTA
                     GVVIAWLGSAVGRLIKPV"
     gene            complement(1215..3023)
                     /locus_tag="VIF63_09210"
     CDS             complement(1215..3023)
                     /locus_tag="VIF63_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239600.1"
                     /translation="MMNIVAPDATPIWSANPWRRYGIEEPPGRRLRLGLLVDDPERLP
                     SWAASSVRLLCDSGLADLVVIATAAGVTSDHARRPGNLGYRLARATIWRTPSDALEST
                     KELGTGARRIRLAVEAGVGNRRRLSPAALQDLRDAQLDLVLRVGADDLDDPGGESATH
                     GTWSFELGSGSRSGGPVGFWEMAGRASEMEIRLVRSSGAGRGTDVLVRGSLAVDPHSY
                     GRTVDRALRACVDLPLQICRDILCGRAPIAGSAMPSAVSSGEPATMADSRGTPGWRAV
                     GRVVASTVLASLARGIYGAFVLKQWSVGRLDGGAARLLSGDVAAARWVDPGGRTRMLA
                     DPAIVPGTSGNAVLCETLDHADGRGRIVRLELSQTSDRGRGGLSVRQEILLDVEGLHL
                     SYPSLATIDDRLLLVPEASGSGGLLLADLAAGATTVADVRHAAGLAAVDPTLFRHAGQ
                     WWLTCTELGPTSGSHLWLFHGPDPRGPWTAHSRNPVVIDAGAARGAGTPFSHEGQLYR
                     PAQDLRAGYGRSVRLMRVLALTHDEYAEELAIELSPDPRSPFPRGLHTVSVDGDAIWV
                     DGYRTVIHPLAGWFRLRARPGAAAAAPGRLVGGWPA"
     gene            complement(3037..4479)
                     /locus_tag="VIF63_09215"
     CDS             complement(3037..4479)
                     /locus_tag="VIF63_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8239601.1"
                     /translation="MTLTAIQPHKIYLAGTWVDSPDVLVVDNPANPTTPAGTTYNATE
                     AQYEQAVNAAVAAFEHTKLLPAYERGRALREISDGIKARREEIGRILSLEAGKPIRDA
                     LVEVDRAVLTFRLGAEEAERMYGETIPLDLMPSSKGRMGITRRFPIGPVAAISPFNFP
                     LNLAAHKLAPAIAAGCSIVLKPPSKDPLTMLTVAEIVDGVGLPAGAVSILPMTRELGD
                     RMVADDRFKLLSFTGSPSVGWRMKERAGKKKVVLELGGNAGVIIDKSADLDWAVKRCV
                     VGAFAYAGQVCISVQRMYVHEDVREAFLEKFMAGVGALNVGDPSDPVTDVGPMVDAGQ
                     AARTQHWVDEAVQLGGRVVAGGHASGAFFEPTVLENVPIEAQVCSNEAFAPLVVVFPF
                     TDFGAAIRQVNDSFFGLQTGVFTNNLTNAWQAFNELEVGGVIINDIPTYRIDHMPYGG
                     VKDSGQGREGLRWSIEDMTELRIMVLAHPQ"
BASE COUNT          730 a         1634 c         1630 g          733 t
ORIGIN      
        1 cgatcagctc ctcggccagc gcacttgcta tggcggcagc gcggttccgg tccgaatcct
       61 gagccgacag cgtgaggagc gtgctgtcaa gttgcgcgct cgagcggacg cgcttgatga
      121 gctcctccgg agtcgttgcc aggccgagtt cgccgatgac ggcgacgagg atcggtcgcg
      181 tcgtcgcgac ggcggcgtac gtcgcggaca gccgctgtga gacgaggagc tgattgaagt
      241 ccgtggcaac tcccgacagg gattggccga cgatcagcgt cgtcttcgcc tcatagacct
      301 tcggttgcag gctgctgacg aggacggcga gaccgccgcc caccaggatg cccgcgacga
      361 gcagcggaaa ccgcgaccgg gcgatcgcga gcaggcggcg gagatccacg gaaatctcac
      421 tctctggccc gcaatcgcgt ggcccatgag cacgtcgcga acagtgcccg gcgacttgtg
      481 cgccgggatc atcgacgagg acgcttggca gcgtcaaatg gcggatcgat gcaacagcca
      541 ggtgctctcc cccgcagacc caactgacgc tgatcgattg ccttggcaca gaattgtcga
      601 cgtggcctcc attgcggctt cgctcccctg gctgctgccc ggcagcatcg ccgcgctcgc
      661 ggtgggcacg ttggccagcc gcgccctggc gcgctggctg ggcgtacgtt ggatcacggc
      721 atgggtcgtc ctcgtgagcc tcggcctcat cctcgccggc acgctgagcc cactggcaac
      781 tggaggcaat gctggccctg gggtcgcccg aacctgtgat ctatcgagga cgtggccggc
      841 ttcgttctca gatctcgctc cgggtaacga cgtagccgtc aatctcgtca tgttctttcc
      901 cctgggctgc gcgctcgggt ttgcgccctt gtcgagacga acgctgctcg tgctcgttgg
      961 ggcgatcgcg ctcccgtttg cgatcgaggc tctccaactt gaggttgccg cactgggccg
     1021 tgcctgccag ggcgctgacg ttgtggacaa cctgacaggt ctcgccgtgg gcctgactgc
     1081 aggcgtcgtc atcgcgtggc tggggtcagc cgtaggccga ttgatcaaac ctgtttgatc
     1141 agccgctgaa gatccgtcgg tccaatgcga ccgtcaaggc cccgccaacg acccgggcac
     1201 cggacgcgga gcggtcaggc cggccagccg ccgaccaatc gacccggtgc tgcggccgcc
     1261 gcgcccggcc gagcccgcag gcggaaccag ccggcaagtg ggtggatcac ggtccgatag
     1321 ccgtcgaccc agatggcgtc accgtcgacc gagacggtgt gaaggccacg agggaacggg
     1381 ctacgcggat cggggctcag ctcgatggcg agctcctccg catactcgtc atgcgtcagc
     1441 gcgagcaccc gcatgagccg taccgaccga ccgtagccgg cgcggaggtc ttgggcgggg
     1501 cggtacagct ggccctcgtg cgagaacggc gtcccggcac cgcgggccgc cccggcgtcg
     1561 atcacgaccg ggttccgcga atgagccgtc cacgggccgc gcggatccgg gccgtggaac
     1621 agccagagat ggctgcccga agtcggcccg agctccgtgc acgtgagcca ccattggccg
     1681 gcgtgcctga acagtgtcgg gtcgaccgca gccaggcccg ccgcatgccg aacatcggcg
     1741 accgtcgtag cgcctgctgc gaggtccgcc agcaggaggc ctccgctacc cgacgcctcc
     1801 gggacgagca acagtcggtc atcgattgtc gccaacgagg ggtacgacag atgcagcccc
     1861 tcgacgtcca gaaggatctc ctggcgtacg gaaaggcctc cgcgaccgcg gtcgcttgtt
     1921 tggctgagct cgagccggac gatccggccc cttccgtcgg catggtcgag cgtctcgcac
     1981 aggacggcgt tgcccgacgt gccaggcacg atggcggggt cggcgagcat gcgggtccgc
     2041 ccacccgggt cgacccacct tgccgcggcg acgtccccgg acagaagcct ggccgcgccg
     2101 ccatcgagac gaccgaccga ccattgcttg agcacgaacg ccccgtagat gccgcgtgcc
     2161 agactcgcga gcaccgtgga cgcgacgacc cggccgacgg cccgccagcc cggcgtgccg
     2221 cgactgtcgg ccatcgttgc cggctcgcca gacgacacgg cggagggcat tgctgaaccg
     2281 gcgattggcg cgcgaccgca gagaatgtcc cggcaaatct ggagcggcag gtcgacgcag
     2341 gcgcgcaggg cgcggtcgac cgtgcggccg tacgagtggg gatccacggc gagactgcca
     2401 cgtaccagga cgtcggttcc cctcccggca ccggacgatc ggacgagccg gatttccatc
     2461 tccgaggctc ggcccgccat ctcccagaat ccgacgggcc cgcccgacct gctgccgctg
     2521 cccagctcga acgaccaggt gccgtgcgtg gcgctctcgc ctccgggatc atcgagatcg
     2581 tcggcgccca cccgtagcac gaggtcgagc tgagcgtccc gaaggtcctg gagggccgcc
     2641 ggcgatagcc ggcggcgatt cccgacaccg gcttcgaccg cgaggcggat ccgccgggcg
     2701 cccgtcccga gctccttggt cgactccagc gcgtccgacg gtgtccgcca gattgtcgcc
     2761 cttgcgaggc ggtacccgag gttgccaggg cgtcgtgcgt gatccgacgt gacgccggcc
     2821 gcagtcgcga tgacgacgag atccgccaga ccgctgtcgc aaaggagccg aacgctgctc
     2881 gccgcccagg acggcagtcg ttcggggtcg tcgacgagca acccgagccg gagccgccgc
     2941 ccgggcggct cctcgattcc gtaacgacgc cacgggttgg cgctccagat cggcgtggca
     3001 tccggcgcga caatgttcat catcttgcct gccggcctac tggggatgcg cgagcaccat
     3061 gatccggagc tcggtcatgt cctcgatcga ccagcgcagg ccctcgcggc cctggccgga
     3121 gtccttgacg ccgccgtatg gcatgtggtc gatgcgatag gtggggatgt cgttgatgat
     3181 gacgccgccg acctcgagct cgttgaacgc ctgccaggca ttggtcaggt tgttcgtgaa
     3241 gaccccggtc tggagcccga agaagctgtc attgacctgg cggatcgctg cgccgaaatc
     3301 ggtgaatggg aagacgacga cgagcggggc gaacgcctcg ttcgagcaca cctgcgcctc
     3361 gatcgggacg ttctcgagca cggtcggctc gaagaaagcg ccgctggcgt gcccgcccgc
     3421 gacgacccgg ccgccgagct ggaccgcttc gtcgacccag tgttgggtgc gcgccgcctg
     3481 gcccgcgtcg accatcggcc caacgtcggt cacggggtcg ctcgggtcgc ccacgttgag
     3541 cgccccgacg ccggccatga acttctcgag gaacgcctcg cgaacatcct catgcacgta
     3601 catgcgctgg acgctgatgc acacctgccc ggcataggcg aaggcgccca cgacgcagcg
     3661 cttcaccgcc cagtccagat ccgcggactt gtcgatgatg actccggcgt tgccgccgag
     3721 ctcgagcacg accttcttct tcccggcgcg ctccttcatc cgccagccga ccgacgggct
     3781 gccggtgaag ctgagcagct tgaaacggtc gtcggccacc atccggtcgc cgagctcgcg
     3841 cgtcatcggc aggatgctga cggcgcccgc gggcagcccg acgccatcca cgatctcggc
     3901 gacggtcagc atcgtcagcg gatccttgga cgggggtttg aggacgatcg agcagcccgc
     3961 cgcgatcgcc ggcgccagct tgtgcgcggc gaggttcagc ggaaagttga acgggctgat
     4021 agcggccacc ggcccgatcg ggaagcgtcg cgtgatgccc atccggccct tcgacgaggg
     4081 catgaggtcg agggggatcg tctcgccgta catccgctcc gcctcctcgg cgccgagccg
     4141 gaaggtgagc accgcgcgat cgacctcgac caaggcgtcg cggatgggct tgccagcctc
     4201 caggctgagg atccgcccga tctcctcgcg gcgcgccttg atcccgtcgc tgatctcgcg
     4261 cagggcgcgg ccgcgctcgt aggccgggag cagcttcgtg tgctcgaacg ccgcgactgc
     4321 ggcgttgact gcctgctcgt actgcgcctc ggtcgcgttg taggtcgtgc cggccggggt
     4381 cgtcgggttg gccgggttgt cgaccacgag cacgtcgggc gaatcgaccc acgtccccgc
     4441 gaggtagatc ttgtggggct gtatcgcggt cagggtcatc ggggctccag gtagcgcacg
     4501 gaagtcatcg catcgtacct ccgactcgcc cggccgacgg aattggccgg cgcggccacc
     4561 gcgcccgtgt tggatcgagg ctatgggccg accttgacgg cgctgaccag gtggcggtcg
     4621 gtgagcccgc cggtggcggc ggagaagccg accaggacgt tggccggcag gctgaccgcc
     4681 gtgtcgagca ccagggtgcc ggcgaccttg acctgcagcc gacccga
//
LOCUS       DATBSW010000340         7386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_350052, whole genome shotgun sequence.
ACCESSION   DATBSW010000340 DATBSW010000000
VERSION     DATBSW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350052"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VIF63_09220"
     CDS             <1..1405
                     /locus_tag="VIF63_09220"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEY8239602.1"
                     /translation="APTLVTNWAQFVNAFGDFTEGSYLAHAVYGYFLNGGGSCYVVRI
                     GGDAPAPAARAELAAAAADAGPGYRVTALESGTDGNKISIDVAPATSGEEGAFNLVVT
                     APSRETETYENVTTKRGKNNVVTMVKERSKLIQLEEIGSASVLERVPATGKVGLSGAD
                     SSKPVRLGAEDYVGDAADRTGFGGLEAIDAVTMLAVPDLMAVYQQGMIDLEGVQAVQL
                     AMIAHCELMGDRVAILDAPPGLNAQQVREWRVDKAGYDSKYATLYWPWIKVFDPLTSQ
                     GKLVPPGGHIAGIWARNDDTRGVHKAPANEVIRGALSLELQITKGEHDQLNPSGINCI
                     RAFPGRGIRVWGARTLSSDPSWRYLNVRRLFNYVEESILEGTQWVVFEPNDLNLWQRV
                     KRTLNAFLVRTWRDGALFGATPGEAFFVKCDAENNPPEVVDAGQLIVEVGIAPVKPAE
                     FVVFRISQFSGGAALSE"
     gene            1440..1898
                     /locus_tag="VIF63_09225"
     CDS             1440..1898
                     /locus_tag="VIF63_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY8239603.1"
                     /translation="MAKGLPNLPSDALASYTFSIEVDRTEIAQFSEVSGLASEVDVIE
                     LKENTLDGKLVIHKAPGAIKPATITLKRAKNASTELWKWHEAVMLGKLGDARRNGSIV
                     MRGFDGSEVARYNFENAWPSKVSMGTLKAGSNEVLMEEVSIVTEALNRVV"
     gene            1902..2324
                     /locus_tag="VIF63_09230"
     CDS             1902..2324
                     /locus_tag="VIF63_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239604.1"
                     /translation="MVGASPALAGAAGEPGYELRTEFPFILPRGFVDQSGVVHRDGVM
                     RLATAKDEIIPQRDPRVRENESYLTVLLLSRVVTRLGRLPEVNPGVIENMFASDLAFL
                     QDLYRRVNQEGTSQAAVSCPSCRHEFTVEMAGDRLGGS"
     gene            2321..2479
                     /locus_tag="VIF63_09235"
     CDS             2321..2479
                     /locus_tag="VIF63_09235"
                     /inference="COORDINATES: protein motif:HMM:NF042798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6760 family protein"
                     /protein_id="HEY8239605.1"
                     /translation="MTYATDQLYEEVAYVAYHLHWGLDAILDLEHPDRRRFVDEIASI
                     NRRLTEED"
     gene            2486..4255
                     /locus_tag="VIF63_09240"
     CDS             2486..4255
                     /locus_tag="VIF63_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY8239606.1"
                     /translation="MRLPWPFRSAQLSVAGSEAAPPGNGASPPRGESGAWRRLPPLGE
                     TIGPPPLVAPARPFAEALAASNPPPAALQPLSHGHSLEAPRGIVVGVARPTPGLSGSA
                     LPRPVQRSPLAALRRSAEAAELEALPESSPSEPAAAPLLAASAATFAAPVPAPVRRLT
                     VASDGVKPPARALTLASTPADAGPRPGLIGRPAAAGSSRPRPVEPAAAIQRAPDAPIQ
                     RAPAAPISSPAPAPDAPRMTLGQARRRGLGAPIGGGPLGAAPSPASVANIAAIAPAPA
                     LPSPDASSFAPLAAVSPPALASVPGPDVQLLPLATPPPSAAATARPVHGDSGPGPSPR
                     PATTGAGPASVHREIRPSRPSSSPAQRRSSPLLSASGLGAVGSQRSPLTIPTGVQRSP
                     SGAATASASGAGQVRVHRGAPAGDMAGAIQARSFTHGGEIYMPDSHGPLTSGPGRALL
                     AHEMTHVAQQRRLGSSLPDEQTSHGRTLEAEAVRAEKSADMPLATPQAGPSSPTPRTT
                     TNTSPALPDLSASPRPSEPKPQRAPAESGGSQGTGGSRSGQGHTEQELEVLAHQLYHR
                     IGRHLRRELLVDRERAGFALDLP"
     gene            4252..4677
                     /locus_tag="VIF63_09245"
     CDS             4252..4677
                     /locus_tag="VIF63_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY8239607.1"
                     /translation="MSESGLGLRFQVKIDGRSFGNWQKCDGLAVEYDIHEYKEGGENS
                     FVHRLPGRVNYPNIRLTRPVDSDSSSVSAWVASLQVRMVPSTCEISVLDPSGESVAQW
                     TLAGVYPIRWTGPTLDVSSNQWATETLELSHNGFLPIGF"
     gene            4679..5371
                     /locus_tag="VIF63_09250"
     CDS             4679..5371
                     /locus_tag="VIF63_09250"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239608.1"
                     /translation="MSRLGAGIPVMGMEKATLAVADGIFSFGSLKFKYNPEQFSLSKR
                     TSWTNDGIKLESDWQMPTYNNTSPATLSLEIFLDAFEELFGDVSGDVKKLLDWTKPGP
                     PFDEPPLLEFRWGMSNVLQGMKFYLESVDATYTLFRVDGTPIRATCRITLTEWSNPAS
                     RQNPTSGGQPGMESHLLGDGESLHSVAWAKYGDASFWRALADFNGIDDPLRVPSGTRL
                     LIPPRRDAAALS"
     gene            5382..7076
                     /locus_tag="VIF63_09255"
     CDS             5382..7076
                     /locus_tag="VIF63_09255"
                     /inference="COORDINATES: protein motif:HMM:NF033848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VgrG-related protein"
                     /protein_id="HEY8239609.1"
                     /translation="MSDRKSVGVALAVSGLDMAPELLAYVEQVVVDEDLHRPAMFAIT
                     MVDPRHDIIERSHVKVGVEIEIATVGQGADDERPLLTGDVVAMECEYDQLGTRIVLRG
                     YAKSHRLHRGRRTRIFQDMTDSDIVEKVAGEARLDIGDIEATSEVHEHVSQANVSDWE
                     FLTDRAKVVGLDLTVVDGQLTFGKRSTSDGAPSEFGTDARHLRFGSNLETFHGRLSGA
                     DQAPDVQVRGWDQDKKQPVTSTASAGTEAAAVDGTDPTELAGYFSAPTFVEVSSPVRS
                     EREAEETAKALAERIGSAFAEAEGTARGSTALRAGAAVRVSGVSGPFNGTYVLTKVRH
                     VLNARGYRSHFTVSGRNDRSLLGLVTGSGNGNGNGHGDGLAGSRAPGFVRGLVADIVD
                     PDRLGRVKVKFPWLDEKYSSAWAPVMQLGAGPESGTFFLPAVDDEVLVGFEQGRIDRP
                     IVVGGLFNKTDKPPNYGQWLDDGAVTGRGIVSRKGHKVTFHDADDIFGVHIVAVDSGK
                     ESAVTIGLNGKDKKLVVQSMGDVDVDAMGDVTLKGAKVTVQATGELVLKGATVKIN"
     gene            7087..>7386
                     /locus_tag="VIF63_09260"
     CDS             7087..>7386
                     /locus_tag="VIF63_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY8239610.1"
                     /translation="MGNPALVTGDKINGTCPAHQIPSASGTAPAGPLPFGAPITQGAI
                     ATVLIGGKPAAVMGSSGYNTPPHAGIVDPPFASPTSQVGRIISGSATVLIGGKMAA"
BASE COUNT         1157 a         2699 c         2526 g         1004 t
ORIGIN      
        1 cgccccgacc ctggtcacga actgggcgca gttcgtgaac gcgttcggcg acttcaccga
       61 gggctcgtac ctcgcccacg ccgtgtatgg ctacttcctg aacggcggcg ggtcgtgcta
      121 cgtcgtccgc atcggcggcg atgcgcccgc gcccgcagca cgggccgagc tcgcggccgc
      181 agccgccgac gccggtcccg gctaccgggt gaccgcgctc gagtcgggca cggacggcaa
      241 caagatcagc atcgacgtcg cgcccgcgac gagcggcgag gaaggcgcgt tcaacctggt
      301 cgtcaccgcg cccagccgcg agaccgagac atacgagaac gtcacgacca agcgcggcaa
      361 gaacaacgtc gtgacgatgg tcaaggagcg ctcgaagctg atccagctcg aggagatcgg
      421 ctcggccagc gtgctcgagc gcgtcccggc aacgggcaag gtcggcctct ccggcgccga
      481 ttcgtccaag ccggtccgcc tcggcgccga ggactacgtc ggcgacgcgg ccgatcgcac
      541 cgggttcggt ggcctcgagg cgatcgacgc ggtgacgatg ctcgcggtcc cggatctcat
      601 ggccgtctac cagcagggca tgatcgacct cgagggcgtc caggccgtgc agctggcgat
      661 gatcgcccac tgcgagctca tgggcgaccg ggtcgcgatc ctcgacgccc ctccgggcct
      721 caacgcccag caggtccgcg agtggcgggt ggacaaggcc ggctacgact ccaagtacgc
      781 gacgctgtac tggccctgga tcaaggtctt cgacccgctg acctcgcagg gcaagctcgt
      841 gccgcccggt ggtcacatcg ccggcatctg ggcccgcaac gacgacaccc gcggcgtcca
      901 caaggcgccg gccaacgagg tgatccgggg cgcgctctcg ctcgagctcc agatcacgaa
      961 gggcgagcac gaccagctca acccgagcgg gatcaactgc atccgggcgt tccccgggcg
     1021 cgggatccgg gtgtggggcg cgcggacgct ctcgagcgat ccgtcgtggc gctacctgaa
     1081 cgtccgccgg ctcttcaact acgtcgagga gtcgatcctc gagggcaccc aatgggtcgt
     1141 cttcgagccg aacgacctca acctctggca gcgggtcaag cggacgctca acgcgttcct
     1201 ggtccgaacg tggcgggacg gcgcgctctt cggggcgacg cctggcgagg cgttcttcgt
     1261 caagtgcgac gccgagaaca acccgcccga ggtcgtcgat gccggccagc tcatcgtcga
     1321 ggtcggcatc gcgcccgtga agcccgccga gttcgtcgtc ttccgcatct cccagttctc
     1381 gggcggtgcg gcgctcagcg aatagtcagc accagagcgc atagaggagg aacccggtca
     1441 tggccaaagg ccttcccaat ctgccgtccg acgcgcttgc gtcgtacacg ttctcgatcg
     1501 aggtcgatcg caccgagatc gcccagttct ccgaggtgag cggcctcgcg tccgaggtgg
     1561 acgtgatcga gctcaaggag aacacgctcg acggcaagct cgtgatccac aaggcgccgg
     1621 gggcgatcaa gcccgcgacg atcacgctca agcgcgccaa gaacgcctcg accgagctct
     1681 ggaagtggca cgaggcagtc atgctcggca agctcggcga cgcccgccgc aacggctcca
     1741 tcgtcatgcg gggcttcgac ggctccgagg tcgctcggta caacttcgag aacgcctggc
     1801 cgagcaaggt ctcgatgggc accctcaagg ccgggtcgaa cgaggtcctc atggaagagg
     1861 tctcgatcgt caccgaagcc ctgaaccggg tggtgtaagc catggtcggc gcaagcccgg
     1921 cgctcgccgg ggccgccggc gagccgggat atgagctacg gaccgagttt ccgttcatcc
     1981 tgccgcgggg cttcgtcgat cagagcggcg tggtccatcg cgatggggtg atgcggctcg
     2041 cgacggccaa ggacgagatc atcccgcagc gcgacccccg cgttcgcgag aacgagtcgt
     2101 acctgacggt cctgctcctg tcccgggtcg tcacacgcct cggccggctg ccggaggtca
     2161 accccggcgt catcgagaac atgttcgcgt cggacctggc attcctccag gacctgtatc
     2221 gacgcgtgaa ccaggaaggc accagccagg cggcggtgtc ctgcccgtcc tgccggcatg
     2281 agttcaccgt cgagatggca ggtgatcgcc tggggggatc gtgacgtacg cgacggacca
     2341 gctctacgag gaagtcgcgt acgtggccta ccacctgcat tggggactcg acgccatcct
     2401 cgacctcgag caccccgacc gtcgccgctt cgtcgacgag attgccagca tcaaccgccg
     2461 gttgaccgag gaggactaac ggagcatgcg gctgccatgg ccgttccggt cggcgcaact
     2521 ctctgtcgcc gggtccgagg ccgccccgcc cgggaacggc gcgtcgccgc cacgcggcga
     2581 gagcggcgcc tggcggcgcc tgccgccgct cggcgagacg atcggcccgc cgccgctcgt
     2641 cgcaccggcg cgcccgttcg ccgaggcgct cgccgccagc aacccgccgc cggcggcgct
     2701 ccagccgctc tcccacgggc acagcctcga ggcgccgcgg ggcatcgtgg tcggcgtcgc
     2761 gcgcccgacg ccggggctct cgggatccgc gctgccgcgg ccggtccagc gatcgccgct
     2821 cgccgcgttg cgccgctccg ctgaggccgc cgagctcgag gcgctgccgg agtcctcgcc
     2881 gtccgaaccg gccgccgcac cgctcttggc ggccagcgct gcgacattcg ccgcgccggt
     2941 acccgcaccg gtccgacggc tcactgtcgc gagcgatggc gtgaagccgc ccgcgagggc
     3001 cctgacactg gcatcgacgc cggccgacgc cggcccaagg cccgggctga tcggccggcc
     3061 tgccgccgcc ggcagctcgc gcccgcggcc cgtcgagcct gcggcggcga tccagcgcgc
     3121 gcccgacgcc ccgatccagc gggcgcccgc cgcgccgatc agttcgccgg ctccggcgcc
     3181 cgatgcgccg cggatgaccc tgggccaggc gcgtcgccgc ggcctgggtg cgccgatcgg
     3241 cgggggaccg ctgggcgctg cgccgtcgcc ggcatccgtg gccaacatcg ccgcgattgc
     3301 gcctgccccg gcgctgcctt cgcccgacgc gagctcgttc gcgcccctcg cggctgtctc
     3361 gccgccggcg ctggcctcgg tgccgggtcc ggacgtgcag ctcctgccgc tcgcgacccc
     3421 gccgcccagc gccgcggcga ccgcccgccc agtccacggc gattctggtc cgggtccctc
     3481 cccccggccc gcgaccactg gcgccggccc agcgtcggtc catcgcgaga tccgcccgag
     3541 ccggccatcg tcgagcccgg cccagcgccg tagctcgccg ctcctctctg cgagcggcct
     3601 cggggcggtc ggcagccagc gctcgccgtt gaccatcccg accggcgtgc agcggagccc
     3661 ctccggtgcc gcgaccgcgt cggcgtccgg tgccggccag gtccgggtcc atcgcggggc
     3721 gcccgcgggc gacatggccg gcgcgatcca ggcgcggtcg ttcacgcacg gtggcgagat
     3781 ctacatgccc gacagccatg ggccgctcac ctccggcccg gggcgcgccc tgctcgccca
     3841 cgagatgacg cacgtcgccc agcagcgccg cctgggcagc agcctcccgg acgagcagac
     3901 gtcgcacggc aggaccctcg aggcagaggc ggtccgcgcg gagaagtccg cggacatgcc
     3961 gctcgcgacg ccccaggccg gcccgtcatc tccgacacca aggaccacga ccaacaccag
     4021 tccggccctg ccggacctga gcgcatcacc gcgcccatcc gagcccaagc cgcagcgcgc
     4081 gcccgccgag agcggcggca gccagggcac cggtggatcc cgctcgggcc agggccacac
     4141 cgagcaggag ctcgaggtcc tcgcccacca gctctaccac cgcatcggcc gccacctgcg
     4201 gcgcgagctg ctcgtcgatc gtgaacgagc cggcttcgcc ctcgacctgc catgagcgag
     4261 tcgggcctcg ggctcaggtt ccaggtgaag atcgatggcc ggagcttcgg caactggcag
     4321 aagtgcgacg gcctggccgt cgagtacgac atccacgagt acaaggaggg cggcgagaac
     4381 agcttcgtcc accgcctccc gggccgcgtg aactatccga acatcaggtt gacccgcccc
     4441 gtcgactcgg actcgtcgag cgtcagcgcg tgggtcgcga gcctccaggt gcgcatggtc
     4501 ccgtcgacct gcgagatctc cgtcctggat ccgagcggcg agtcggtcgc gcagtggacc
     4561 ctcgcgggcg tgtacccgat ccggtggacc gggccgacgc tggacgtgag cagcaaccag
     4621 tgggcgaccg agaccctcga gctcagccac aacggcttcc tgccgatcgg attctgagat
     4681 gagtcgcctc ggcgccggca ttccggtcat gggcatggag aaggccaccc tcgcggtggc
     4741 cgacggcatc ttttcgttcg gctcgctcaa gttcaagtac aacccggagc agttctcgct
     4801 ctcgaagcgc accagctgga cgaacgacgg gatcaagctc gagtcggact ggcagatgcc
     4861 gacctacaac aacacgtcgc ccgccacgct cagcctggaa atcttcctgg acgccttcga
     4921 ggagctgttc ggggacgtgt ccggggacgt gaagaagctc ctggactgga cgaagcccgg
     4981 gccgccattc gacgagccgc cgctgctcga attccgatgg gggatgagca acgtcctcca
     5041 ggggatgaag ttctatctcg agtcggtgga tgcgacgtac accctgttcc gggtcgacgg
     5101 caccccgatc cgggcgacct gcaggatcac gctcaccgag tggtccaacc ctgcctcacg
     5161 ccagaacccg acctcgggcg gtcagccggg aatggagtcc cacctgctcg gcgatggcga
     5221 gtcgctccac tcggtcgcct gggccaagta cggcgatgcc agcttctggc gggcgctcgc
     5281 cgacttcaac ggcatcgacg acccgctgcg cgttccgtcc ggcacgcgcc tcctcatccc
     5341 gccgcggcgc gacgccgcgg cgctgtcgta ggcggcccgc gatgagtgac cgcaagtcgg
     5401 tcggcgtggc gctcgcggtg agcgggctcg acatggcgcc cgagctgctc gcgtacgtcg
     5461 agcaggtcgt cgtcgacgag gacctgcacc ggccggcgat gttcgcgatc acgatggtcg
     5521 acccgcgcca cgacatcatc gagcgcagcc acgtgaaggt cggcgtcgag atcgagatcg
     5581 ccacggtcgg ccagggcgcc gacgacgagc gcccgctgct caccggcgac gtggtggcga
     5641 tggagtgcga gtacgaccag ctcggcacgc gcatcgtgct gcgcgggtac gccaagtcgc
     5701 atcgcctgca ccgcggccgc cgaacgcgga tcttccagga catgaccgac agcgacatcg
     5761 tcgagaaggt cgcgggcgaa gcccgcctgg acatcggtga catcgaggcg acgtcggagg
     5821 tgcacgagca cgtcagccag gcaaacgtca gcgactggga gttcctcacc gatcgcgcaa
     5881 aggtggtcgg cctcgacctc accgtggtcg acggccagct cacgttcggc aagcgctcga
     5941 cctcggacgg ggcgccgtcc gagttcggca cggatgccag gcacctgcgc tttggctcca
     6001 atctcgagac gttccacggt cgcctgagcg gcgccgacca ggcgccggac gtgcaggtcc
     6061 gcggctggga ccaggacaag aagcagccgg tcacctcgac cgcatcggcc ggcaccgaag
     6121 cggcagcagt cgacggcacc gacccgaccg agctcgccgg gtacttcagt gcgccgacct
     6181 tcgtcgaggt cagctcaccg gtccggagcg agcgcgaggc cgaggagacg gcgaaggcgc
     6241 tcgcggagcg catcggcagc gcgttcgccg aggccgaggg cacggcgcgc ggtagcacgg
     6301 ccctgcgcgc cggcgcggcc gtgcgcgtca gcggcgtcag cgggccgttc aacggcacgt
     6361 atgtgctcac caaggtgcgc cacgtgctca atgcccgcgg ctaccgcagc cacttcaccg
     6421 tcagcggccg gaacgatcga tcgctgcttg gcctcgtcac cgggagcggc aacggcaacg
     6481 gcaatggtca cggcgacggc ctcgccggca gccgcgcacc cggcttcgtc cgcggtctcg
     6541 tcgcggacat cgtggaccct gacaggctcg gccgggtcaa ggtcaagttc ccctggctcg
     6601 acgagaagta cagctccgcc tgggcgccgg tgatgcagct cggcgccggg cccgagagcg
     6661 ggacgttctt cctgccggcc gtggacgacg aggtcctcgt cgggttcgag cagggacgca
     6721 tcgaccggcc gatcgtcgtc ggtggcctct tcaacaagac cgacaagccg ccgaactacg
     6781 ggcagtggct cgacgacggc gccgtcaccg gccgcgggat cgtgtcccgg aagggccaca
     6841 aggtcacgtt ccacgacgcc gacgacatct ttggcgtcca catcgtcgcg gtcgattccg
     6901 gcaaggagtc cgccgtgacc atcgggctga acggcaagga caagaagctc gtcgtccaga
     6961 gcatgggcga cgtcgacgtc gacgccatgg gcgacgtcac gctcaagggg gcgaaggtca
     7021 ccgtgcaggc gacgggcgag ctcgtcctca agggcgccac cgtgaagatc aactaggagc
     7081 gcgaacgtgg gcaatccagc gctcgtgacc ggcgacaaga tcaacggcac ctgcccggcg
     7141 caccagatcc cgtccgcgtc cggcacggcg ccggcgggcc cgctgccatt cggcgcgccg
     7201 atcacccagg gcgcgatcgc gaccgtcctg atcggcggca agcccgccgc ggtcatgggc
     7261 tcgtccggct acaacacgcc gccccatgcc ggcatcgtcg atccgccatt cgcgtcaccc
     7321 acgagccagg tcggccggat catcagcggc agcgcgaccg tgctcatcgg cggaaagatg
     7381 gcggcc
//
LOCUS       DATBSW010000341         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_843602, whole genome shotgun sequence.
ACCESSION   DATBSW010000341 DATBSW010000000
VERSION     DATBSW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843602"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VIF63_09265"
     CDS             <1..1073
                     /locus_tag="VIF63_09265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEY8239611.1"
                     /translation="ADALDARWGPIGAGTGARLALDSVYFGGGTPSLLPAEAINRLLG
                     LIRRRFGIAPGAEVTLEANPGPDERGDAGALRAAGVTRLSIGAQSMDAAELRALGRRH
                     TPDDVAATVHAARDAGIESISLDLLYDVPGQTLDTWATTLDAALATEPDHVSMYALTL
                     DDPDAEGITGPNGDHLPVSDGARRWRERARVGQDDDRAAQMYELADQRLASAGFDWYE
                     ISNWARPGHESRHNVAYWQGRSWQAVGPGAHAFDGRTRRWNAARLDRYMDALQPTDGA
                     PPRLPPGSASTSGAEESEVSAAMLALRTRSGLKPTASQAPTIAEAVAWGLEAGLLAEN
                     PDCVVLTMRGRLLANELFVRMV"
     gene            1089..1517
                     /locus_tag="VIF63_09270"
     CDS             1089..1517
                     /locus_tag="VIF63_09270"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8239612.1"
                     /translation="MIEAVHRTFRVEVSPAEAWARLAQIDRWPEWAPHITAATVSPAG
                     ALGPASTGALRIRGLGRTAFRMTVWEPGRRWTWVGAMPGATVTYEHGFAPAGEAATTL
                     DWVVSLDGPLAFVIRPLFARIYGRNLDRAIPRLKAWIATA"
     gene            1540..2760
                     /locus_tag="VIF63_09275"
     CDS             1540..2760
                     /locus_tag="VIF63_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239613.1"
                     /translation="MATMKPAAPVTGPSTGRGLAVAVLLGGLGVAVVVTLLGLTTYGL
                     PGSYESTPFGDLGAFVFEATLLWLGYVLRTRRPENALGWLFLVFGLCATFSHVAWATM
                     QLSYLPGGDRNVGAAVSWLGAVGSILTWTYLMTSLVIRFPDGRPDSRGEARMLRWLPL
                     FCVVAAGGAAIRPGPLLIFPAFDNPISTPPALDGLLTTASTLALLAVLIPTVVSGRAI
                     IRRYRGATTTERLQLRWFAFGATLSLTATAIYLVFGVILAPEDHVIREGTYALFVAAL
                     ASMPIAVFQAIATHRLYDIDRIIGRTLAYGALTAILAGLYSASIRLFNWLFVDLTGQQ
                     SEVALVLTTLILATSFTPIKAWLEKGAAKRFKLDQAPAPVGSDTPGATAAPFTAEQQA
                     YIDARIEAAVRASR"
     gene            2856..>3626
                     /gene="hrcA"
                     /locus_tag="VIF63_09280"
     CDS             2856..>3626
                     /gene="hrcA"
                     /locus_tag="VIF63_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY8239614.1"
                     /translation="MARRIRRSTGPLDLRSQAILRAVIEEYVTTAQPVGSQALVDRYR
                     LGVSSATVRNILAELELGGLLSHPHTSAGRIPTDAGYRWFVESIIDSAPLPEVEQLMI
                     RHQFGQVEFASEHWFRLAASTIAGLTHAAGIATPAKPAAARVRRVDLVSINDHMASLV
                     VVLREGSIKQSLVTLASSEDQSALTSVAAVLNELVHDATAEQAEATLARLDPALPHAL
                     LVARVGERIVRLLADYDREAVEEVFSDGLLNVMGAPEFA"
BASE COUNT          523 a         1324 c         1201 g          578 t
ORIGIN      
        1 gggccgatgc ccttgatgcg cgatggggcc cgatcggtgc tggcaccggc gcgcgactcg
       61 cgctcgactc cgtgtacttc ggtggcggga cgccgagcct cctgcccgcc gaggcgatca
      121 atcgcctgct cggtctgatc cgtcgtcggt tcggcattgc ccccggcgcc gaggtgacgc
      181 tcgaggccaa ccccggaccc gacgagcgcg gagatgccgg cgcgctccgg gctgcgggcg
      241 tgacgcggct gtcgatcggc gcgcagagca tggacgcggc ggagctgagg gccctgggac
      301 gccggcacac gcccgacgac gtcgccgcga ccgtgcacgc cgctcgcgac gccggcatcg
      361 agtcgatcag cctcgacctg ctgtacgacg tccccggcca gacgctcgat acgtgggcta
      421 ccacactgga cgctgccctc gccaccgagc cggatcatgt gtcgatgtat gcgctgaccc
      481 tcgacgaccc ggatgccgag gggatcaccg ggccgaacgg tgaccacctg cccgtcagcg
      541 acggcgccag gcgctggcgc gagcgcgccc gggtcggcca ggacgacgac cgcgccgcac
      601 agatgtacga gctcgcggac cagcgcctgg catcggccgg cttcgactgg tacgagatca
      661 gcaactgggc gcgcccgggc cacgaatccc ggcacaacgt cgcgtactgg cagggccgca
      721 gctggcaggc cgtcgggccg ggggcgcatg cattcgatgg ccgaacccgc cgttggaacg
      781 ccgcccgcct cgatcgctac atggacgcgc tgcagccaac tgatggtgca cctcctcgac
      841 tgccgcccgg cagcgcgtcg acctcgggcg ccgaggaatc ggaagtctct gcggccatgc
      901 tcgccctgcg gacacggtca ggactcaaac cgacggccag ccaagcgccc acaatcgccg
      961 aggccgtcgc ctggggcctg gaggcgggtc tgctggcgga gaatccagac tgcgtcgtgc
     1021 tcacgatgcg cggccggctg ctggccaacg agctcttcgt gcgcatggtc taggagaggg
     1081 aggctgcgat gatcgaagcg gtccaccgga ccttccgcgt cgaggtgtcg cctgcggagg
     1141 cgtgggcaag gctcgcccag atcgatcgct ggccggagtg ggcgccacac atcaccgccg
     1201 ccaccgtgtc cccggccggc gcgctcgggc ctgcatccac tggggcgctc cggatccgtg
     1261 gcctcgggcg gaccgcgttc cggatgaccg tctgggaacc cgggcggcgc tggacatggg
     1321 ttggggcgat gccaggcgca acggtcactt acgagcacgg attcgcgccc gcgggcgaag
     1381 cggcaacgac gctcgattgg gtcgtgtccc tggatggccc actggcgttc gtcatccggc
     1441 ccctcttcgc gcggatctac ggacgcaacc tcgaccgcgc cattccgagg ctcaaagcct
     1501 ggatcgcaac cgcctagcca gcgctaggat cgcgccggga tggcgacgat gaaaccagcc
     1561 gcgcccgtca ccggtccgtc caccgggcgg gggctcgcgg tcgccgtcct gctgggcggg
     1621 ctcggcgtcg ccgtggttgt gacgctcctc gggctcacca cctacgggct cccaggcagc
     1681 tacgagtcca cgccattcgg cgacctgggc gcgttcgtct tcgaggccac cctcctgtgg
     1741 ctcggatatg tgctccgcac gcggcgcccg gagaacgcgc tgggctggct gttcctggtg
     1801 ttcgggttgt gcgccacctt ctcgcacgtg gcatgggcga cgatgcagct cagctatctg
     1861 ccgggtggcg accggaacgt tggcgcagcg gtctcatggc tcggcgcggt cggatcgatc
     1921 ctcacctgga cctacctgat gacgtccctg gtgatccgct ttcccgatgg ccgcccggac
     1981 agccgcggcg aggcgcggat gctccgctgg ctgccgctct tctgtgtcgt ggcagctggc
     2041 ggagccgcga ttcgacccgg gcccctgctg atctttccgg cattcgacaa tccgatttcc
     2101 acgccgccgg cgctcgacgg attgctcacg accgcgtcca ccctcgcgct ccttgcggtg
     2161 ctcattccca cggtcgtctc gggtcgagca atcattcgtc gatatcgcgg cgcaacaacg
     2221 acggagcgac tgcagcttcg ctggttcgct tttggggcga ccctttccct gaccgcgaca
     2281 gcgatctacc ttgtgttcgg ggtcatcctg gcgccggagg accacgtcat ccgcgaggga
     2341 acctacgcac tgttcgtggc cgcgctcgcc agcatgccga tcgccgtgtt tcaggcaatc
     2401 gcgacgcacc ggctctacga catcgaccgg atcatcgggc ggacgcttgc gtacggggca
     2461 ctgacggcca tcctcgccgg cctctattcc gcatcgatcc gactcttcaa ctggctgttc
     2521 gtcgatctca cgggccagca gtcagaggtt gcgctcgtcc tgacgacgct gatccttgcc
     2581 acgtcattca ccccgatcaa ggcgtggctt gagaagggcg cggcgaagcg gttcaagctc
     2641 gaccaggcgc ccgcgccggt tgggagcgac acacccggcg ccaccgccgc cccattcacc
     2701 gcggaacagc aggcctacat cgacgcacgc atcgaggccg ccgttcgagc ctcgcgttga
     2761 cactcccgac tgctcgggct acgatcctca tcactgcgtt agcactctgg catggagagt
     2821 gctaactaag cagcacccga acgggagaac aggccttggc gcgtcggatc cgtcgctcca
     2881 ccggccctct ggaccttcgc tcgcaggcga tcctccgggc cgtcatcgag gaatacgtca
     2941 ccaccgcgca gccggtcggc agccaggcgc tcgtcgaccg gtatcgcctc ggcgtgtcca
     3001 gcgcgaccgt gcgcaacatc ctcgccgagc tcgagctcgg cggcctcctg agccaccccc
     3061 acacctcggc cgggcgcatc ccgaccgatg ccggctaccg ctggttcgtc gagtcgatca
     3121 tcgactccgc gccgctgccc gaggtcgagc agctgatgat ccggcaccag ttcggccagg
     3181 tcgagttcgc cagcgagcac tggttccgcc tcgccgcgtc gaccatcgcc gggctcacgc
     3241 atgccgccgg gatcgcgacg ccggcgaagc ccgctgcggc gcgcgtccgc cgcgtcgacc
     3301 tcgtctcgat caacgaccac atggcgagcc tcgtcgtggt cctgcgcgag ggctcgatca
     3361 agcagtcgct cgtcaccctc gcgtcgtcgg aggaccagtc ggccctcacc tcggtggccg
     3421 cggtcctcaa cgagctcgtc cacgacgcca ctgcagagca ggccgaggcc accctcgccc
     3481 gcctggatcc cgccctgccc cacgcgctgc tggttgcccg cgtcggtgag cggatcgtcc
     3541 gcctgctcgc ggactacgac cgcgaagccg tcgaggaggt cttcagcgac ggcctgctca
     3601 acgtgatggg cgcccccgag ttcgcg
//
LOCUS       DATBSW010000342        10539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_138690, whole genome shotgun sequence.
ACCESSION   DATBSW010000342 DATBSW010000000
VERSION     DATBSW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138690"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VIF63_09285"
     CDS             complement(<1..314)
                     /locus_tag="VIF63_09285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239615.1"
                     /translation="MPTTIRPITAEELPAWFQAFGTAFYIWASDPHAAAESRRDHMDL
                     TRALAAFEDGAIVGTYRSFPVQLTVPGGVRIPVSGVSAVSVRPTHRRQGLLTRMITED
                     LS"
     gene            434..964
                     /locus_tag="VIF63_09290"
     CDS             434..964
                     /locus_tag="VIF63_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239616.1"
                     /translation="MGDMTMDELRGWFAGRIPDDWFTGPLDVTSDREEILVVGELAAV
                     KAAKGTSEAEAAAARESRIDGFREDTRATRMHIAEEAQRRFRRHVSWGVTLGGETEHF
                     TTAAIPVMTRLRMRERGVLDTLVDAGVARSRSEALAWCVRLVGDNESEWIDKLRGALG
                     AVKEARGTGPKPGRHN"
     gene            1084..1701
                     /locus_tag="VIF63_09295"
     CDS             1084..1701
                     /locus_tag="VIF63_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8239617.1"
                     /translation="MIESERRDRAADQAYRDHANDVYRVAYGILRDPDEALDATHDTF
                     ARAWERWDQYDSQRPLRGWLHGIVVHVSLDQLRRRRVRRLALPQLGRSPVARLETSTA
                     DIADDVVRREVVDRALDELKPSVRATLVLRHYYGYDYAEIGSMLGVSSGTVGSTLSRA
                     HAVLRERLEAQAGAPAQPATASTTSITLRSRTRRDAATEPPEVLP"
     gene            1698..3443
                     /locus_tag="VIF63_09300"
     CDS             1698..3443
                     /locus_tag="VIF63_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239618.1"
                     /translation="MTHDPRRPSAAESFDDDTIGKLVRETAADWTMPPVRLDAPAWRD
                     RVRGARARRVAVVQGWFGRVGQAATAAVALTVAAALVAVILTGPPPAPGKSPEPSTGA
                     TPGATSGALPSPLPKLFVDGEVPEPTTLLVQVEPGDLARVDLTKGVLGRAVTGAQFGS
                     GISVHEDGSIVCLCVTRAGSVDGMPTQAIITFKRFDEAGSLIASAPVDTISGVPDPRD
                     VGKPYVDRPSHVLTSIGSSADGRYGFVGWSKRAPPVWQSGVLVVDLADGTVVSRLALP
                     DEGTGDGDGRRVIDAPSVVGTTFFLRLLIARSWYEWSPVSSENPSYDFHSDAFAASFK
                     GGDLTGAERLAAAADCGEVVTRGGVAGDSTWLGCQSGGGSTTVIRRLAGDGTKIDDVR
                     VFGIGGIDADLTAVSPDGAYLYAWNPISATLTRVDLATGETTTAQGAAQAAVERGPLA
                     AFGEWLAPTAAAKVWLLGGMAVSPDGSRVYALGIANPASEAGGSTGVYAFDGNAMASV
                     GHWQPTADFVSLAVSPDGRFVYAAGLPGVDAKGNSMSQQSASITVFDASDGSPRLIAG
                     ALGRGNITFTSSVFD"
     gene            3466..3591
                     /locus_tag="VIF63_09305"
     CDS             3466..3591
                     /locus_tag="VIF63_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239619.1"
                     /translation="MATRMNGSSFDGRTDRVSHHEKGKAAADKKPATKKPAVKKK"
     gene            complement(3663..4271)
                     /locus_tag="VIF63_09310"
     CDS             complement(3663..4271)
                     /locus_tag="VIF63_09310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8239620.1"
                     /translation="MPSRARNRATSGEPPHLWLVRHGETEWARLGRHTGRTDIPLTAL
                     GREQATSLSRRLAGHVFAQVWSSPLSRALDTARIAGFGDRVETDDDLLEWDYGDDEGR
                     TTPEIRADRPGWTIWDNGPKAGELIDDVAARVDRVIARARQERGDVLVFAHGHVLRIL
                     GARWIGQAPSEGRLFALATATISELGWEREQPVIERWNDHTD"
     gene            complement(4272..4589)
                     /locus_tag="VIF63_09315"
     CDS             complement(4272..4589)
                     /locus_tag="VIF63_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8239621.1"
                     /translation="MTEPPVVVRVAHLEIDPAQLEAFIAAVREEIEEAVRVEPGVLAI
                     YAVAERDHPARLQFFEIYADDAAYAAHRESPHFRTYLATTNSMIRRRELFEMVPIQLS
                     SKA"
     gene            complement(4586..5182)
                     /locus_tag="VIF63_09320"
     CDS             complement(4586..5182)
                     /locus_tag="VIF63_09320"
                     /inference="COORDINATES: protein motif:HMM:NF017949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8239622.1"
                     /translation="MTDPIADIIGDYGAFAAQQRDRLSARGIDIASYELSHIAYRVPE
                     WDQYVRVRTQLERHAVANRENVWNGRPISLIRFDGLEVLDGKSLPMIELIPPVHQRVY
                     KMGCEHLGVVVGEAGFDAFVEANKPVLTGQQFQGPNSIPDPVYILMEDFTHVKFYRLS
                     LQASVEVFEGPFASGFRHVEDWVPQRLVTATGPNPLPR"
     gene            complement(5232..5912)
                     /gene="msrA"
                     /locus_tag="VIF63_09325"
     CDS             complement(5232..5912)
                     /gene="msrA"
                     /locus_tag="VIF63_09325"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8239623.1"
                     /translation="MFLFSKPDKLPSPADALPGRPDPILSPRPHTVLRTPIAGPYPDG
                     FEIAEFALGCFWGEEKTFWQQPGVWTTAVGYQGGYTPNATYQEACSGKTGHAEAVRVV
                     FDPSKTSYTQLLKVFWENHDPTQGMRQGNDMGTQYRSAIFVQDDAQRTAAEASRDMYQ
                     ERLTARGYGTITTEIVGPPAPKFYFAEDYHQQYLDKNPGGYCPNHSTGVRLPDDFSIP
                     ATELQYAE"
     gene            6068..6991
                     /locus_tag="VIF63_09330"
     CDS             6068..6991
                     /locus_tag="VIF63_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8239624.1"
                     /translation="MSEATLERPIGGSDVLHAPTAVVPPVERTDDSPRHPRSIDPRTA
                     ALGLPRGDLTAWQRSVRHFMARVIAFVVVRLWFKVELVGAERLPAGPAVYCFNHLSWF
                     DPLALTTALPGKPRIFFYGPKEPDLRKGKRNRFMWWFGICVPFSPLKDDLLTSIRWVQ
                     AVFDTNGVLAIAGEGTIHVHEGDLLPFQEGSAYLALRGGVPLVPIAITGTAWAHFRGK
                     ITIRIGDPIETGERPTRQTIAHYSARIWHAIRAMVADDRDAAPPTSRIERRVSDLFND
                     WGPGGRQAAARQRGPNPVDVPIPPLPSRGAD"
     gene            7059..7424
                     /locus_tag="VIF63_09335"
     CDS             7059..7424
                     /locus_tag="VIF63_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239625.1"
                     /translation="MSGTGLSADPQEYRARLDDQSDDQIDRWVSELMRDVVIRRGIVK
                     VVADFRSAARLSEAEFERVFAFGGGAPRTLGRDRQGHVVVPTISLWTLVPGLRGLVPD
                     SRDRLIAYLVANFGELVYV"
     gene            7528..8418
                     /locus_tag="VIF63_09340"
     CDS             7528..8418
                     /locus_tag="VIF63_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY8239626.1"
                     /translation="MLNAAATAAIGALAGADPATFVRLAIAMLALQCSIGALNDLVDE
                     PLDAREKPGKPIPAGEATRRAAAVVAVAGGVLGVALSAASGLPTAVAALGCLGLGYVY
                     DVRLSRTALSWLPLSLALPLLPIHAWLGATGSIPPGLVTLGPVAILAGAGLSLANGLV
                     DLERDARGGRRAVTVALGRGRTWLLQTVALGGAVVLALWLAPTGLPTDPAPAAAGFLI
                     DPGILRAVRTFGVPIGSAVIGAGAFVLRSQRPALRERGWELEAIGVAGVGLGWLCGTA
                     LSAAGAAALAAIGGGAAAQA"
     gene            8562..9062
                     /locus_tag="VIF63_09345"
     CDS             8562..9062
                     /locus_tag="VIF63_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239627.1"
                     /translation="MRSISFRLRSVLIALVGLAMSASLTFGAQPPAAASWGLANASSH
                     AGKTVPVADGDVETAGDEETGEEEIGEETGEETGEEEGTEDAGDHCLTDPTGLTEEEL
                     AAVNHGSIVCWAAHQTTWPEEFRNHGAFVSSWAHAGKDSASKDAAKAARDAAKDARNA
                     AKGKGN"
     gene            complement(9185..10210)
                     /locus_tag="VIF63_09350"
     CDS             complement(9185..10210)
                     /locus_tag="VIF63_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8239628.1"
                     /translation="MARTDFRAAAARRLSEERELSPAIVSLLSNDRQGRSRGGVRHVD
                     VDRITPNPEQPRLAMEQVSLDELTASIREHGVLQPILVRPRENGEFELIAGERRWRAS
                     MAAGLATIPALVEEIDDDTALEISIIENLQREDLSPLDEAAMYDRMVKDHGYSVRRLA
                     QKLGKDKGYLENRLRLADAPDEVRELVSLRKDTLSHAYELMKVDDPRKRKRLAAQVAR
                     GELTLIKLRDKIEGRPARSTPGPDAGDEEPAYADADGDETAEEASAAWTGRRHDIAAA
                     NLTENSLVAAKQQLAEAVEGLLDVLRAPDVVRGIGTVDRANLAKYLTIAKLRLENAIA
                     VVRSDGA"
     gene            complement(10197..>10539)
                     /locus_tag="VIF63_09355"
     CDS             complement(10197..>10539)
                     /locus_tag="VIF63_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8239629.1"
                     /translation="GVIIPVQTQYYAMKGLTNLVKVINTIRLKLNRRLKILGLLPTFF
                     DARTNLARDMLDELRVVGDHHVFSAIIKNTVKLGEAPLVGRPITSYATSSEAARTYRE
                     LAREVVELGAN"
BASE COUNT         1523 a         3542 c         3811 g         1663 t
ORIGIN      
        1 ctcgacaggt cctcggtgat catccgggtc agcaggccct gccgccggtg ggtcgggcgg
       61 acgctcacgg cggagacgcc gctcacgggg atgcgcacgc cgccgggaac ggtgagctgg
      121 accgggaagg agcggtaggt cccgacgatc gcgccgtcct cgaatgcggc gagtgcccgg
      181 gtgaggtcca tatggtcccg ccgggactcg gcggcggcat gcgggtcgct cgcccagatg
      241 tagaacgccg taccgaatgc ctggaaccag gcgggcagct cctcggccgt gatcgggcgg
      301 atggtcgtgg gcatcccggg acgatacgcg atcacccccg tggtcgcttg gggtcgcttg
      361 cggtggtcgt gcaagcgtgt aatcatgtaa ttcaacttgc gacgaagttg cgcgaggcac
      421 tgggagacga acgatgggcg acatgacgat ggatgagctg cgcggctggt tcgccggccg
      481 gatcccggac gactggttca ccggacccct ggacgtgacg tcggaccgcg aggagatcct
      541 cgtggtcggc gagctcgccg cggtcaaggc ggcgaagggg acgagcgagg ccgaggcggc
      601 ggcagcgcgc gaatcgcgca tcgatggctt ccgcgaggac acgcgcgcga cgcgcatgca
      661 catcgccgag gaggcccagc gccgattccg ccggcacgtg tcctggggcg tcacgctcgg
      721 cggcgagacc gagcacttca ccacggccgc aatcccggtc atgacccgac tccggatgcg
      781 cgagcgcggc gtgctcgaca ccctcgtcga tgcgggcgtg gcgcgaagcc gcagcgaggc
      841 gctcgcctgg tgcgttcggc tcgtcggcga caacgagagc gagtggatcg acaagctccg
      901 tggcgcgctc ggtgcggtca aggaagcgcg cggcaccggt ccgaagccgg gacgccacaa
      961 ctgagccttc ggcgggcgcg atcgctcgcc aacctgcgag agcgaccgaa ccgtccgcca
     1021 tgatgcccag gtgggcatct gcgccagacg caaggaatcg cgaaccgcgg ccgtaggtag
     1081 gtcgtgatcg agagcgagcg gcgcgaccgc gctgccgacc aggcctaccg cgaccacgcg
     1141 aacgacgtgt accgcgtcgc ctacggaatc ctgcgcgacc ccgacgaggc actcgacgcg
     1201 acgcacgaca cgttcgcgcg tgcgtgggag cgctgggacc agtacgacag ccagcggccg
     1261 ctccggggct ggctccatgg gatcgttgtc cacgtctcgc tcgaccagct ccgccggcgg
     1321 cgcgtccgcc ggctcgccct gccgcagctc ggccgctcgc cggtggcgcg gctcgagacc
     1381 tcgaccgccg acatcgccga tgacgtcgtc cgccgggagg tggtcgatcg cgccctcgac
     1441 gagctgaagc cgtcggtccg ggcgacgctc gtcctgcgcc actactacgg ctacgactac
     1501 gccgagatcg ggtcgatgct cggggtttcg agcgggacgg tcggctcgac gctgtcccgc
     1561 gcccatgccg tcctccgcga acgcctcgaa gcccaggcgg gcgcgcccgc ccagcctgcg
     1621 acggcctcga cgacgtcgat cacgctcaga tcccgaaccc ggcgcgatgc cgccacagaa
     1681 ccgcccgagg tgctcccatg acccacgacc cgcgccggcc gtccgcggcc gaatcgttcg
     1741 acgacgacac gatcggcaag ctcgttcgcg agaccgccgc cgactggacg atgccgccgg
     1801 tgcgcctcga cgcgcccgcg tggcgggatc gcgtccgcgg cgcccgcgcg cggcgcgttg
     1861 ccgtggtgca gggatggttc ggtcgcgtcg ggcaggccgc cacggcggcc gtagccctca
     1921 cggtcgctgc ggcgctcgtg gcggtgatcc tcacgggtcc accgccggct ccgggcaaga
     1981 gcccggagcc ctcgaccggc gccacgccgg gagccacgtc cggcgccctt ccgagtccgt
     2041 tgccgaagct gttcgtcgac ggcgaggtcc cggagccgac gaccctcctg gtccaggtcg
     2101 agccgggcga cctcgcccga gtggatctca ccaaaggtgt gctcgggcgc gccgtgaccg
     2161 gcgcccagtt tgggagtggc atctccgtcc acgaggacgg cagcatcgtc tgcctgtgcg
     2221 tgacccgcgc gggcagcgtc gacggcatgc ccacgcaggc gatcatcacg ttcaagcgct
     2281 ttgacgaggc gggcagcctc atcgcatcgg cgcccgtcga cacgatctcg ggggtgccgg
     2341 atccgcgcga tgtcggaaag ccatatgtgg accgcccatc gcacgtcctg acatcgatcg
     2401 gctcgagcgc cgacggtcgc tatggcttcg tcggctggag caaacgcgcc ccgccggtct
     2461 ggcagagcgg tgtccttgtc gtcgacctgg ccgacgggac ggtggtgagc cggctcgccc
     2521 tgccagatga gggaacgggc gacggcgatg gacggcgcgt gatcgacgcg ccctccgttg
     2581 tcgggacgac gttcttcctg cgtctcctga tcgcgcgctc ctggtacgaa tggagcccgg
     2641 tgtcgtcgga gaacccgtca tacgacttcc atagcgacgc cttcgccgcg agcttcaagg
     2701 gcggagacct caccggcgcc gagcgccttg cggccgctgc cgactgcggc gaggtcgtca
     2761 cccgcggcgg cgttgcgggt gattcgacgt ggctcggctg ccagtcgggc ggcgggtcga
     2821 cgaccgttat ccggcggttg gccggcgacg gcacgaagat cgatgacgtc cgcgtgttcg
     2881 gcatcggcgg catcgatgcg gacctgaccg cggtcagccc tgacggggcg tacctctatg
     2941 cctggaaccc gatctcggcg accctgaccc gagtcgatct ggcgaccggc gagacgacga
     3001 ccgcgcaggg tgctgcccag gcggccgtcg aacgagggcc gctcgcggcg ttcggcgagt
     3061 ggctcgcccc gaccgcagcc gcaaaggtct ggctgctcgg cgggatggcg gtctcgccgg
     3121 acggctcgcg ggtctacgcg ctcggcatcg cgaatccagc gtccgaagcc ggcgggtcga
     3181 ccggggtgta cgccttcgat gggaacgcaa tggccagcgt cggccactgg cagcccaccg
     3241 ccgacttcgt ctcgctcgcc gtgagccccg atggccgatt cgtgtatgcc gcagggctgc
     3301 cgggcgttga tgccaagggc aacagcatga gccagcagtc cgcgtcgatc acggtgttcg
     3361 acgcgagcga cgggagcccg cgcctcatcg ccggtgccct cggccgcggc aacatcacct
     3421 tcacgtcgag cgtgttcgac tgatctgccg gaggtaccct cggcggtggc gacccgaatg
     3481 aacgggtcga gcttcgacgg gaggaccgat cgggtgagcc atcacgagaa gggcaaggcc
     3541 gcagcggaca agaagccggc gacgaagaag cccgcggtga agaagaagta ggcacgctgg
     3601 gcccgacatc gggtcctgga tgaatcgaag ggggccgagg tggccccctt cttcatgccc
     3661 gctcagtccg tgtggtcgtt ccagcgttcg atgacgggct gctcccgctc ccagccgagc
     3721 tccgagatcg tcgcggtcgc cagcgcgaac agccggccct cgctgggcgc ctggccgatc
     3781 cagcgcgcgc cgaggatccg gagcacgtgt ccgtgcgcaa agacaaggac gtcgccccgc
     3841 tcctggcggg cccgggcgat gacccgatcg acgcgtgccg ccacgtcgtc gatcaactca
     3901 cccgctttcg ggccgttgtc ccagatggtc cagccgggtc ggtccgcccg gatctcgggg
     3961 gtcgtgcgac cctcgtcgtc gccgtagtcc cactcgagca ggtcatcgtc cgtttcgacg
     4021 cgatcgccaa agccggcgat ccgcgccgtg tccagcgctc gcgacagggg actggaccag
     4081 acctgggcaa acacatggcc ggcgaggcgg cggctgagtg acgtcgcctg ctcccgtccg
     4141 agcgcggtca gcgggatgtc ggtgcgtccg gtatgccggc cgagacgagc ccattcggtc
     4201 tcaccgtggc gaacaagcca aaggtgcggc ggctcgccgg aggtggcccg gttgcgagcc
     4261 cggctcggca ttcaggcctt cgagctgagc tggatcggaa ccatctcgaa aagctcccgc
     4321 ctgcgaatca tcgagttcgt cgtcgcaagg taggtccgga aatgcggtga ttcgcgatgc
     4381 gcggcgtatg cggcgtcgtc ggcgtagatc tcgaagaatt gcagccgggc agggtggtcc
     4441 cgttcggcga cggcatagat ggccaggaca cccggctcca cgcgaaccgc ctcctcgatc
     4501 tcctccctga ccgcggcgat gaacgcctcg agctgcgctg gatcgatctc gagatgcgcg
     4561 acgcggacaa cgacgggcgg ctcggtcacc ggggcagcgg gttcggcccg gtcgccgtga
     4621 ccagccgctg cgggacccag tcttcgacgt ggcgaaaccc actcgcgaac gggccttcaa
     4681 acacctccac ggatgcctgc agcgagagtc gatagaactt cacgtgggtg aagtcctcca
     4741 tcaggatgta gacggggtcg ggaatgctgt tcgggccctg gaattgctgt cccgtcagga
     4801 cgggcttgtt ggcctcgacg aacgcatcga accccgcttc gccgaccacg acgccgaggt
     4861 gctcgcagcc catcttgtag acgcgttgat ggacgggcgg gatcagctcg atcatcggga
     4921 gcgacttgcc gtcgagcacc tcgagcccgt caaagcggat caacgagatc gggcggccgt
     4981 tccagacgtt ctcgcggttg gcaacggcgt ggcgttcgag ctgggtccgc acccgcacgt
     5041 actggtccca ctcgggaacc cggtacgcga tgtggctgag ctcgtacgaa gcgatatcga
     5101 tgccgcgcgc ggacaggcgg tcgcgctgct gcgccgcgaa cgcgccgtag tcgccgatga
     5161 tgtccgcgat cgggtcggtc accggttcac ctccggtgac atcgtacggg ctgcatcccg
     5221 ttcagggatg cctactcggc gtattggagc tcggtcgccg ggatcgagaa gtcgtccggc
     5281 aggcggacgc cggtgctgtg gttcgggcag tagccgccgg gattcttgtc gaggtactgc
     5341 tggtggtagt cctcggcgaa gtagaacttg ggcgcgggcg gtccgacgat ctcggtcgtg
     5401 atcgtgccgt agcccctggc ggtcagccgc tcctggtaca tgtcgcgcga ggcctcggcg
     5461 gcagtgcgct gggcatcgtc ctggacgaag atcgcggatc ggtactgggt gcccatgtcg
     5521 ttgccctggc gcatgccctg cgtcgggtcg tggttctccc agaacacctt gagcagctgc
     5581 gtgtacgagg tcttcgaggg atcgaagacg acgcgcaccg cctcggcatg gccggtcttg
     5641 cccgagcacg cctcttggta ggtggcgttc ggcgtgtagc cgccctggta gccgacggcg
     5701 gtggtccaca cgccgggctg ctgccagaac gtcttctcct cgccccagaa gcagccgagc
     5761 gcgaactcgg cgatctcgaa gccgtccggg tacgggcccg cgatcggcgt tcgaaggacg
     5821 gtgtgcggac gcgggctcag gatcgggtcg gggcgtccgg gcagggcgtc ggcgggggag
     5881 ggcagcttgt cgggcttcga gaacaggaac atcgagtcac gctccttggc gcggtttggt
     5941 tcgggagggt tcgaactgta ggcccgaacg gtgacggatc gatgtcaggc gcccgtgctc
     6001 gctcgcgcga tgacgcgaat ggtaggctcg gacccgcgtg ggagggttcc cgcgcgggag
     6061 ggttgggatg agcgaggcaa cgctcgagcg cccgattggc ggcagcgacg tgctgcacgc
     6121 cccgacggcg gtcgtgccac ccgtggagcg tacggatgat tcgccgcgcc atccgcgctc
     6181 gatcgatccc aggacggcgg cgctcggcct gcctcgtggc gacctcacgg cgtggcagcg
     6241 gagcgtccgg cacttcatgg cccgcgtcat tgcgttcgtc gtggtccggc tgtggttcaa
     6301 ggtcgagctc gtcggggcag aacggctccc ggccggcccg gccgtgtact gtttcaacca
     6361 tctcagctgg ttcgacccgc tcgcgctcac cacggcgctc ccgggcaagc cgcgcatctt
     6421 cttctacggg ccgaaggaac cggacctgcg caagggcaag cgcaaccgat tcatgtggtg
     6481 gttcgggatc tgcgtcccgt tcagtccgct caaggacgac ctcctgacct cgatccggtg
     6541 ggtgcaggcg gtcttcgaca cgaatggcgt gctcgccatc gccggcgagg gcacgatcca
     6601 cgttcacgaa ggcgatctgc tgccgttcca ggaggggtcc gcctacctgg cgctccgcgg
     6661 cggcgtccca ctcgtgccga tcgcgatcac cggcacggca tgggcgcact tccgcggcaa
     6721 gatcacgatc cgcatcggcg acccgatcga gacgggggag cgaccgacgc gccagacgat
     6781 cgcccactac tccgcgcgca tctggcacgc gatccgggcg atggtcgccg acgatcgcga
     6841 tgctgcgccg ccgacgtcgc ggatcgagcg cagggtgagc gacctgttca acgactgggg
     6901 accgggcggg cgacaggccg cagcccggca gcgcgggccg aatcccgtgg acgtcccgat
     6961 cccgccgctc ccatcgcgcg gggccgactg acggcggtcg actgaggggc gccgcctgag
     7021 gggcgctgcg cgcacctcgt ctggcatact cggccgcgat gagcggcacc gggctttcgg
     7081 ccgatcccca ggaatatcga gcacgcctgg acgaccagtc cgacgaccag atcgaccgct
     7141 gggtctcgga gctcatgcgc gacgtcgtga tccggcgggg gatcgtgaag gtcgtcgccg
     7201 acttccgatc ggccgctcgc ctgagcgagg ctgaattcga acgggtgttc gcttttggcg
     7261 gtggagcgcc gcggacgctc ggccgggatc gccaggggca cgtcgtcgtg ccgacgatca
     7321 gcctgtggac gctcgttccg gggctccgcg gcctcgtgcc cgacagccgg gatcgcctga
     7381 tcgcgtacct cgtcgccaac ttcggggagc tcgtgtacgt ctgacccggc ggcagaccgg
     7441 ttcagcaccg ggtcggttgc gacccgaggg ccttcgtggc ggacttcgat cggcgggctg
     7501 atccggctcg tccacccgtt tccgtccctg ctcaacgcgg cggcgaccgc ggcgatcggc
     7561 gccctggccg gggcggatcc cgcgaccttc gtgcggctgg cgatcgcgat gctcgcgctg
     7621 cagtgctcga tcggcgcgct gaacgacctg gtcgacgagc cgctcgatgc ccgggagaag
     7681 ccgggcaagc cgatcccggc gggggaggcg acacgccgtg ctgctgcggt ggtcgcagtg
     7741 gcgggcggtg tcctgggcgt cgccctgtcg gcggcctcgg gacttccgac cgcggtcgcg
     7801 gccctcggct gcctcgggct cggctacgtc tacgacgtgc ggctgtcgag gacggcgctg
     7861 tcgtggctgc cgctctcgct cgcgctcccg ctcctgccga tccacgcctg gctgggcgcg
     7921 acgggttcga tccctccggg gctggtcacg ctcgggccgg tcgccatcct cgccggcgcc
     7981 ggcctgtccc tcgccaacgg tctcgtcgat ctggagcgag atgcgcgagg cgggcgccgc
     8041 gccgtgaccg tcgcgctcgg ccgagggcga acgtggctgc ttcagaccgt cgccctcggc
     8101 ggcgctgtgg tgctggccct gtggctggcg cccacggggc tacccaccga cccagcgcct
     8161 gcagcagccg gcttcctgat cgatccgggc atccttcggg cggtccggac gttcggggtg
     8221 ccgatcggct cagctgtcat cggggctggg gcgttcgtgc ttcgcagcca gcgtccagcg
     8281 ttacgggagc ggggctggga gctcgaggcg atcggcgtcg cgggcgtggg ccttggctgg
     8341 ctctgtggga cggcgctgtc ggcagcgggc gcggcagccc tcgcggcgat cggtggggga
     8401 gcggcggcgc aggcctgagc gtcgaccagc gcggttcgct ccgatgcgcg atcggcgggc
     8461 ggggcggagc cgcaccggac caaggtactg gtccctaacg gcagcgccgg acgagcgtta
     8521 tgttcggtga agcgttcacc atcactcggg gagcctgaac cgtgcgatcc atctcgttcc
     8581 gccttcgatc cgtgctcatc gcccttgtcg gcctggccat gtcggcgagc ctcaccttcg
     8641 gcgcgcagcc gccggccgct gcctcgtggg ggctcgcaaa tgcctcgagc cacgcgggca
     8701 agaccgtccc ggtcgcggat ggcgacgtcg agaccgccgg cgacgaggag accggcgagg
     8761 aggagatcgg cgaggagacc ggagaggaga ccggggagga ggaggggacc gaggacgcgg
     8821 gcgaccactg cctgaccgac ccgaccggcc tcaccgagga agagcttgcc gccgtgaacc
     8881 acggctcgat cgtgtgctgg gcggcgcacc agacgacctg gcccgaggaa ttcaggaacc
     8941 acggtgcctt cgtgtcgagc tgggcccatg ccggcaagga ctcggcgagc aaggacgcgg
     9001 ccaaggccgc gcgggatgct gccaaggacg ccaggaacgc cgccaagggc aagggcaact
     9061 agcgggcggc gactgccgcc gttgattcag acagcaagag agccccgctc ggggatcaac
     9121 ccggcgaggc tctcttgatt cctggtcgtg aactcgccgc tcgtggcggc cctcgtttgg
     9181 tggctcaggc tccgtcgctt cgcacgaccg cgatcgcgtt ctcgaggcga agcttggcga
     9241 tcgtgaggta cttggccagg ttggcccggt cgaccgtgcc aatgccccgt acgacatccg
     9301 gcgctcggag gacatccagc agcccttcga ccgcctcggc aagctgctgc ttcgcggcaa
     9361 cgagtgagtt ctcggtgagg ttggccgctg cgatgtcgtg gcgccggccg gtccaggcgg
     9421 cggaagcctc ctcggccgtc tcgtcgccgt cggcgtccgc gtacgcgggc tcctcgtcac
     9481 cggcgtcggg tccgggagtt gagcgggccg ggcgtccttc gatcttgtcg cgaagcttga
     9541 tgagggtgag ctcgccacgg gcgacctgcg cggccaggcg cttgcgcttg cgcgggtcgt
     9601 ccaccttcat cagctcatag gcgtgcgaga gcgtgtcttt gcgcaaggac accagctcgc
     9661 ggacctcgtc gggagcatcg gcgagtcgca gccgattctc gaggtagccc ttgtccttgc
     9721 cgagcttttg ggcgagacgt cggaccgagt agccgtggtc cttcaccatc cggtcgtaca
     9781 tcgccgcttc gtcgagcggg gacaggtcct cgcgctgcag gttctcgatg atcgagatct
     9841 cgagcgccgt gtcgtcgtcg atctcctcga cgagcgccgg gatcgtggca agcccggccg
     9901 ccatcgacgc gcgccatcgg cgctccccgg cgatgagctc gaactcgccg ttttcgcgcg
     9961 ggcgaacgag gatcggctgg aggacgccgt gctcgcggat cgacgccgtc agctcgtcca
    10021 ggctgacctg ctccatggcg agacgaggtt gctcgggatt cggcgtgatg cgatcgacgt
    10081 cgacgtgacg gaccccgccc cgtgatcgac cctggcggtc gttcgagagg aggctgacga
    10141 ttgccggcga gagctcgcgc tcctccgaca ggcgccgcgc ggcggcggcg cggaagtcag
    10201 ttcgcgccaa gttcgaccac ctctcgtgca agctcgcggt aggtccgcgc ggcctcggac
    10261 gacgtcgcat agctcgtgat cgggcgcccg acgagcggtg cctcgcccaa cttcacggtg
    10321 ttcttgatga ttgcgctgaa gacatggtgg tcgccgacga cgcgcagctc gtcgagcatg
    10381 tcgcgggcaa ggttcgtcct ggcgtcgaag aaggtgggca ggaggccgag gatcttcagg
    10441 cgccggttca gcttgagtcg aatggtgttg atgaccttga cgaggttcgt caggcccttc
    10501 atcgcgtagt actgcgtctg tacggggatg atcacgccg
//
LOCUS       DATBSW010000343         4793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_878899, whole genome shotgun sequence.
ACCESSION   DATBSW010000343 DATBSW010000000
VERSION     DATBSW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878899"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VIF63_09360"
     CDS             <1..724
                     /locus_tag="VIF63_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8239630.1"
                     /translation="TAAAASRVGLEAHIVVGGPPVEGSPNLGLMEQLGATVHQTATDS
                     RAEREAMVNRVASDARAAGRHVKVLPVGGSEPAGAWGHVLAAIEVAGQAATHGFEPAA
                     IILPTATGGTQAGLIVGSALAFEKAPRIAGILVARTADELRPIVAGLVDELAAMAGIA
                     TPAIAIEIDDTHLGGGYGEPSVAASKAAVMLARTEGVLVDPVYTAKGLAGLIALVRSG
                     AIDGEHAIFWHGGGLPALFEPR"
     gene            complement(740..1225)
                     /locus_tag="VIF63_09365"
     CDS             complement(740..1225)
                     /locus_tag="VIF63_09365"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8239631.1"
                     /translation="MDKITPTLWFDGNAEEAAEFYTSLFPNSRVGKVERAASDTPSGP
                     EGMVITVAFTLDGREYVGLNGGPDFKFTEAVSLSIDCADQAEVDHYWAKLVEGGGEYS
                     VCGWLKDRFGLSWQVIPRALPELLASPDRAAAKRVMEAMLQMTKLDVAELERAAQPTA
                     A"
     gene            1306..1536
                     /locus_tag="VIF63_09370"
     CDS             1306..1536
                     /locus_tag="VIF63_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239632.1"
                     /translation="MDEEPEPTPDIDYYDTGAVRYRGFQLDGEMHGAWEFLRKDGSLM
                     RAGRFDRGKQVGVWKTFDRAGMVVKETDFSKG"
     gene            1578..2342
                     /locus_tag="VIF63_09375"
     CDS             1578..2342
                     /locus_tag="VIF63_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8239633.1"
                     /translation="MPETAFDRHGSQIRWTELPGPLPPRVFVHGLGSNGEWNLGKVAR
                     DPALGAHRSLIVDLPGHGSSDRPADFPYSLEAHADAVAAACTAAGVEGVDLIGHSLGG
                     DVSIVTAFRHPELVGRLVISEANLDALPPSLTGRASQAIRAQTEDDFVATGYHELMAR
                     FPEWAETLVRAAPYAVHRSATQLNIGTKPTMREMFTGMAIPRTFIHGDHGEPLLDADG
                     LRRSGVRIVTIPDAGHMMMFDNPAAFMAALVEALAT"
     gene            complement(2346..3104)
                     /locus_tag="VIF63_09380"
     CDS             complement(2346..3104)
                     /locus_tag="VIF63_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239634.1"
                     /translation="MSDDSRIGGVRGRLQPQANGPAKVGDPNVLTGEAKPNRLSNETA
                     PSKPIAVSASTLSTQARPGTSQTTQATQATPPRTWTPSAPRPQPIPVQADEPARRRIS
                     LPTLIFLGFLALTGFRLLNEFAQSFGDSTPTPTPAAAVAATPRPAGPVTFGTDMSDSC
                     EISGQAASFTNGTDVWWLARMPTRQSKDAIVVVRVLFEGDQIVRDVWPPDGTTFWTVM
                     CDGPVSQDKPGSYRVEVWNEDETVLQATGEYEVR"
     gene            3223..3555
                     /locus_tag="VIF63_09385"
     CDS             3223..3555
                     /locus_tag="VIF63_09385"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8239635.1"
                     /translation="MTVDQLSSVFGALADPTRRAILARLASGDLAVRDLAAPFPMSQP
                     AISRHLKVLEGAGLISRSRQATARLSHLEPEPLREATTWLARYQAFWDERFDQLDEVL
                     ASLKSKQP"
     gene            3576..4064
                     /locus_tag="VIF63_09390"
     CDS             3576..4064
                     /locus_tag="VIF63_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8239636.1"
                     /translation="MGKTRITAPPGQPFIDMERDFDAPRELVHKAYLDPELVTQWLGP
                     RKYEMVLSQWDARDGGAYRYVHRDGQSEHGFRGVFHSMSIDNMVQTFEYEGAPGHVSL
                     DVQSIEDLGNGRSRVKSHSVFMSVADRDAMVEAGMGDGVEDGYNRLDELLAKLQPVGA
                     SH"
     gene            4377..>4793
                     /locus_tag="VIF63_09395"
     CDS             4377..>4793
                     /locus_tag="VIF63_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239637.1"
                     /translation="MAGEPIRIALSGAAPDAVALDGDTAWVLSGEGGTLMEVDIVAGR
                     EVRAIDVGFGATHLALPIPGVAAVGRFDDSGTGSYLVFVDRATGRTVGATTNELGAMA
                     GGEAGVVWALEKGGRLVKVDAETRTVIDAAAIQVGQN"
BASE COUNT          684 a         1713 c         1658 g          738 t
ORIGIN      
        1 caccgctgct gccgcgtccc gggtcgggct cgaggcgcac atcgtggtcg gcgggccccc
       61 ggtcgaaggt tcgccgaacc tgggcctgat ggagcagctc ggcgcgacgg tccatcagac
      121 ggccaccgac tcgcgcgcgg agcgagaagc gatggtcaac cgtgtcgcgt cggacgcccg
      181 agccgcgggc cggcacgtca aggtcctgcc cgtcgggggc tccgagccgg ccggcgcgtg
      241 gggccacgtc cttgccgcga tcgaggtggc cggccaggcg gcgacccatg gcttcgagcc
      301 cgccgcgatc atcctgccaa ccgcgacggg cggcacccag gcgggtctca tcgtcggatc
      361 cgcgctggcg ttcgagaagg cgccgcggat cgcgggcatc ctcgtcgccc gcaccgccga
      421 cgagctgcgg ccgatcgtcg ccggcctggt cgacgagctt gccgcgatgg cggggatcgc
      481 cacgcccgcg atcgccatcg agatcgacga cacgcacctc ggaggcggct acggcgagcc
      541 gtcggtggcc gcgtcgaagg ccgccgtaat gctcgcgcgg accgaggggg tcctcgtcga
      601 cccggtctat accgccaagg gccttgccgg gctcatcgcg ctggtgcgct ccggcgcgat
      661 cgacggcgag cacgcgatct tctggcacgg tggcggcctg ccggcgctgt tcgagccgcg
      721 ctagcggcgc cggtgacggt caggcggctg tcggctgcgc ggcgcgctcc agctcggcga
      781 cgtcgagctt ggtcatctgc agcatcgctt ccatgacccg tttcgcagcc gcccgatccg
      841 ggctcgcgag gagctccggc agcgcgcgcg ggatgacctg ccacgacagg ccgaagcggt
      901 ccttgagcca gccgcagacg ctgtactcgc cgccgccctc gaccagcttc gcccagtagt
      961 gatcgacctc cgcctggtcg gcgcagtcga tcgagagcga gacggcctcg gtgaacttga
     1021 agtccgggcc gccgttcagc ccgacgtact cgcgcccgtc gagggtgaac gcgacggtga
     1081 tgaccatccc ctccggcccg ctcggcgtat ctgacgccgc gcgctcgacc ttgccgacgc
     1141 ggctgttggg aaacaggctc gtgtagaact cggcggcttc ctcggcgttg ccgtcgaacc
     1201 acagggtggg cgtgatcttg tccatcgggg cctccgctcg gtgcgagccc cgatcctagt
     1261 cgcggcgcac gacaccggca ttgcagcctc tgtatcgtgc tgcggatgga cgaggaacct
     1321 gagccgacac ccgacatcga ctactacgac accggcgcgg tccgctatcg cggcttccag
     1381 ctggacggcg agatgcacgg cgcgtgggag ttcctgcgca aggacggcag cctgatgcgc
     1441 gccgggcggt tcgaccgcgg caaacaggtc ggcgtgtgga agaccttcga tcgggccggc
     1501 atggtcgtca aggagacgga cttctcgaag ggctgagcgg cccgcagggc agcggcggag
     1561 ccgcgtacgc tgcgggcgtg ccggagaccg ccttcgatcg acacggttcg cagattcggt
     1621 ggaccgagct gccgggtccg ttgccgcccc gcgtgttcgt gcacggcctg ggcagcaacg
     1681 gcgagtggaa cctcggcaag gtcgcccgag atcccgcgct gggcgcccat cgttcgctga
     1741 tcgtcgacct gccgggccac ggctcgagcg atcgaccggc ggacttcccg tactccctcg
     1801 aggcgcacgc cgatgcggtc gctgccgcgt gtaccgcggc cggggtcgaa ggcgtcgacc
     1861 tcatcggcca cagcctcggc ggtgatgtct cgatcgtcac cgcgtttcgg catcccgagc
     1921 tcgtcgggcg gctcgtgatc tcggaggcga acctcgatgc gctgccgccg tcgctgaccg
     1981 ggcgggcgag ccaggcgatc cgcgcgcaga ccgaggatga cttcgtcgcc accggctacc
     2041 acgagctgat ggccaggttc ccggagtggg ccgagaccct ggttcgtgcg gcgccatacg
     2101 cggtccatcg cagcgcgacc cagctcaaca tcggcacgaa gccgacgatg cgcgagatgt
     2161 tcacgggcat ggcgatcccg cggacgttca tccacggcga tcacggcgag cccctcctcg
     2221 atgcggacgg cctgcgccga tccggcgtgc ggatcgtcac gattcccgac gccggtcaca
     2281 tgatgatgtt cgacaacccc gcggcgttca tggccgcgct ggtcgaggcc ctcgcgacgt
     2341 agggctcagc ggacctcgta ctcgccggtg gcctggagca cggtctcgtc ctcgttccag
     2401 acctcgaccc ggtagctgcc gggcttgtcc tggctcaccg gcccgtcgca catgaccgtc
     2461 cagaacgtcg tgccgtcggg cggccagacg tcccgcacga tctgatcgcc ttcgaacagg
     2521 acccgaacga ccacgatcgc atccttgctc tggcgcgtcg gcatccgcgc gagccaccac
     2581 acatcggtcc cgttcgtgaa gctggcggcc tgaccgctga tctcgcacga gtcggacatg
     2641 tccgtgccga aggtgacggg tcctgccggt cgcggggttg ccgccacggc agcggccggc
     2701 gtcggcgtcg gcgtcgagtc gccgaacgac tgggcgaact cgttgaggag acggaatcca
     2761 gtcagggcca gaaagccgag gaagatgagc gttggcaacg agatccgtcg gcgcgccggc
     2821 tcgtccgcct ggaccgggat cggctgcggc cggggcgccg agggcgtcca cgtcctcggt
     2881 ggtgtcgcct gcgtcgcttg cgtcgtctgc gacgtccccg ggcgcgcctg cgtcgacagg
     2941 gtgctcgccg agacggcgat cggcttggac ggggccgtct cgttggacag ccggttcggc
     3001 ttggcctcgc ccgtcaggac gttcggatcc cccaccttgg cgggtccgtt cgcctggggc
     3061 tgaagccgcc ctcgaacgcc accgatcctc gagtcgtcgc tcatgccgat gctcaccgta
     3121 cggcatggag cgctcttgcg gaccactgat ttttcaaggg gttgacatgc gctcgccggt
     3181 cgggtttaat aaccaaacca tttcataaca tggtggttat agatgacggt cgatcagctc
     3241 agctcggtgt tcggggcgct cgcggatccc acccgccgcg cgatcctcgc ccgcctcgcg
     3301 tcaggcgacc tggcggttcg ggacctcgcg gcccccttcc ccatgtccca gcccgcgatc
     3361 tcgcgccacc tcaaggtgct cgagggcgcc ggcctgatct cccgcagccg gcaggccacg
     3421 gcccgcctca gccacctcga accggagccg cttcgcgagg cgacgacctg gctcgcgcgc
     3481 taccaggcct tctgggacga gcgcttcgac cagctggacg aagtcctcgc ctcgctcaaa
     3541 tccaagcagc cgtaacagga aggaaggaac tcgacatggg caagaccagg atcaccgcgc
     3601 cgccgggcca gccgttcatc gacatggagc gcgacttcga cgccccgcgc gagctcgtcc
     3661 acaaggccta cctcgacccc gagctcgtca cccagtggct cgggccacgc aagtacgaga
     3721 tggtcctctc gcagtgggat gcccgcgacg gcggcgccta tcgctacgtc catcgcgacg
     3781 gccagagcga gcacgggttc cgcggcgtct tccattcgat gtcgatcgac aacatggtcc
     3841 agaccttcga gtacgagggc gctccgggcc acgtctcgct cgacgtccag tcgatcgagg
     3901 acctcggcaa cggccgcagc cgcgtgaagt cgcactcggt cttcatgtcc gtcgccgacc
     3961 gtgacgccat ggtcgaggca ggcatgggcg atggcgtcga ggacggctac aaccgcctcg
     4021 acgagctgct cgccaagctc cagccagtcg gcgcctcgca ctgaccttcc ccgccggctc
     4081 ggccggcact ccacccgcgt gttccagcgc cccgtccgat cttcgggcgg ggcgcatccg
     4141 ttccccgaag gtgtcacgga tgcccgtggc cggggatcga ggctgtcgag atggtggagc
     4201 tgcgtcgatc tcgctcgatt ccagcgatgg cgggcctgtg cctcgccgtc gtcggctgcg
     4261 gtggaccagc cgcctcaacc tcgcagccgc cgctggcgtc aacggccacg ccgcacgccg
     4321 cttccacggc gccggattcg acgcccacgc gagcgccggc ggcgtccgcc aagcccgtgg
     4381 ccggtgaacc gatccgaatt gcgctctcgg gcgcggcccc ggatgccgtc gcgctcgacg
     4441 gcgacaccgc ctgggtcctc tccggcgagg gcggcacgct catggaggtc gacatcgtcg
     4501 cgggccgcga ggtccgcgcc atcgatgtcg ggttcggggc gacccacctc gcgctgccga
     4561 ttcccggcgt cgccgccgtc ggtcggttcg acgacagcgg gaccggctcg tacctcgtct
     4621 tcgtggaccg tgccaccgga cgcacggtcg gcgccaccac gaacgagctg ggagcgatgg
     4681 ccggcggcga ggcgggcgtc gtctgggcgc tggagaaggg cggccgcctg gtcaaggtgg
     4741 atgcggagac ccgcaccgtg atcgatgccg cggcgatcca ggtcggccag aac
//
LOCUS       DATBSW010000344         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1197203, whole genome shotgun sequence.
ACCESSION   DATBSW010000344 DATBSW010000000
VERSION     DATBSW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197203"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VIF63_09400"
     CDS             <1..915
                     /locus_tag="VIF63_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239638.1"
                     /translation="GQQQRWQDPVAFGAMAIGAIALVAFPFLMRRPHPLVPLGLFRIR
                     SFSTINLATLLIYGALYASGGFQALFLQNTLGYTPLGAAIVGLPSGILLTILSTRVGS
                     LSGRLGVRPFLIAGPLVMAAGSLWWLTVQSTAKPWAASLGNPASLVPPIDALIGPLPA
                     GIIFGIGISLLVAPLTTALMGSVPVRNSGIASAINNALSRVGQPLVAAAVFIAVSGTF
                     YAALASAVPGTDPNAPELRAYDALNPPPKGAPVELVEATKVASTAAYHTAVIVSALLL
                     VAGAVVNAVGLRQPGRGRAPSAERAPPS"
     gene            complement(1009..1446)
                     /locus_tag="VIF63_09405"
     CDS             complement(1009..1446)
                     /locus_tag="VIF63_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239639.1"
                     /translation="MPMIQAFWNKLNGNEKLVGYGAILVVVALLAAIASTRASADVTT
                     SVIAAVLIFVIFWIKNSKMKIPWPVPESTVVLGIAAVATVIALLKFLPALAFITRLDG
                     IALFLNLAGCGVMAFGAWKEYQAKLPKSAPPASSPPPAPPPAA"
     gene            complement(1520..1921)
                     /locus_tag="VIF63_09410"
     CDS             complement(1520..1921)
                     /locus_tag="VIF63_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239640.1"
                     /translation="MVQAFWNKLNGNEKMVGYGAVLIVVAFLAATASGFGAGPETTSL
                     IVAVVIFAIYWTKQSNMKIPWPVPLTTVVLGIAAVAAVLALLALLGVLSAITRLDGIA
                     SLLNFAGCGVMAFAAYKEYQATLPKATPPAA"
     gene            complement(2018..>2669)
                     /locus_tag="VIF63_09415"
     CDS             complement(2018..>2669)
                     /locus_tag="VIF63_09415"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEY8239641.1"
                     /translation="AGVGRDRVTGIVLAGGRSSRFGTEKLAATSDGQTLLERAVERVE
                     SVAGTILIAGPVEPTTRFRHVPPVRFIDDAEPFAGPLAALAGTLREIATEFAIVVGAD
                     MPGLVPAVLQSMLDRLAMSADIDAVLLDAVPGDARGDGPPKQQVLPVALRVGPASETA
                     AASLAAGDRSLVRFLGRLRVAEIPVEEWLQLDPVGESTFDIDIPVDLERFRAREIR"
BASE COUNT          423 a          908 c          925 g          413 t
ORIGIN      
        1 ggccagcagc agcgctggca ggatcccgtc gcgttcggcg cgatggcaat cggcgccatc
       61 gccctcgtcg ccttcccgtt cctgatgcgc cggccgcacc cgctggtccc gctcggcctg
      121 ttccggatcc gatccttcag cacgatcaac ctcgcgacgc tcctgatcta cggcgccctc
      181 tacgcgagcg gcggattcca ggcgctgttc ctgcagaaca cgctcggcta cacgccgctc
      241 ggcgcggcga tcgtcgggtt gccgtcgggg atcctgctga cgatcctgtc gacccgggtc
      301 gggtcgctgt cggggcggct cggcgttcgg ccgttcctga tcgccgggcc actggtcatg
      361 gccgccggct cgctgtggtg gctgaccgtc cagtcgacgg cgaagccgtg ggcggcgagc
      421 ctcggcaacc ccgcgtcgct ggtgccgccg atcgatgccc tcatcgggcc gctgccggcg
      481 ggcatcatct tcgggatcgg gatctcgctc ctggtcgcgc cgctcacgac ggcgctcatg
      541 ggctccgtgc ctgtgcgcaa ttccgggatc gcatccgcga tcaacaacgc cctctcacgg
      601 gtcggccagc cgctcgtcgc ggcggcggtg ttcatcgccg tgagcgggac gttctacgcg
      661 gccctcgcgt cggccgtccc gggcactgac ccgaacgccc ccgagctccg cgcctacgac
      721 gcactgaacc cgccacccaa gggcgcccct gtcgagctgg tcgaagcgac gaaggtcgcc
      781 tcgaccgccg cgtaccacac ggcggtgatc gtgtcggccc tgttgctcgt cgccggcgcg
      841 gtggtgaacg cggtcggcct gcgccagccc ggacgagggc gagcgccttc agccgagcgc
      901 gcgccgccga gctgaacgaa cgggccaggc cggcgccgca cgcggaaacg aaacgggccg
      961 ggggacgaac ccccgacccg tcgctgggca gctggtgagc gcgagccgtt acgccgcggg
     1021 cggcggcgca gggggtggag acgatgccgg cggagccgac ttgggcagct tcgcctggta
     1081 ttccttccag gcgccgaatg ccatcacgcc gcaaccggcg agattgagga acagcgcgat
     1141 cccgtcaagt cgcgtgatga acgcgagcgc gggcaggaat ttcaggagcg cgatgacggt
     1201 cgcgaccgcg gcgatgccga ggacgacggt tgattccggg actggccacg ggatcttcat
     1261 cttcgagttc ttgatccaga agatcacgaa gatcaggacc gcggcgatga cgctcgtcgt
     1321 gacgtccgct gacgcacggg tagacgcaat cgcggcaagc agcgcgacca cgacgaggat
     1381 cgccccgtag ccaacgagct tttcgttccc gttgagcttg ttccagaacg cctgaatcat
     1441 cggcatggct tcctcccgga aaagcgacgg gccggggatg agcccggccc gtcttgggca
     1501 gctggtgagc cctactcggt cacgccgcgg gcggggtcgc cttgggcagc gttgcctggt
     1561 attccttgta cgcagcgaac gccatgacgc cacatcctgc gaagttgagc aggctggcga
     1621 tgccgtcgag tcgcgtgatg gcactcagga cgcccagcag ggcgaggagc gcgagcactg
     1681 cggcgaccgc ggcgatgccg aggacgacgg tcgtaagcgg cacgggccac gggatcttca
     1741 tgttcgactg cttcgtccag tagatcgcga agatcacgac ggccacgatg aggcttgtcg
     1801 tttcgggacc agccccgaaa ccgctggcgg ttgcggcgag gaacgcgacg acgatgagga
     1861 cggcgccgta ccccaccatc ttctcgtttc cgttgagctt gttccagaac gcctggacca
     1921 tgggcattga tcatccctcc ctgacaacgg ctgacggctc acccgccgcg cgtgcagcgg
     1981 agagcgtaac gaacggcgcg gcagatcgga aggggtttca gcgaatctcg cgagcacgaa
     2041 atcgctcgag atcgacggga atgtcgatat cgaacgtgga ttcgccgaca ggatcgagct
     2101 gcaaccactc ctcgacggga atttccgcca ccctgaggcg gccgagaaat cgcaccagtg
     2161 agcggtctcc ggcagccagc gaggcggcgg cggtttcgct tgccggcccg acgcggagcg
     2221 ccacgggcag gacctgctgc ttcggtgggc catcgccgcg cgcatcgccg ggtacggcgt
     2281 cgagcaggac tgcatcgatg tcggcgctca tcgcgagtcg atccagcatc gactggagca
     2341 cggcaggcac gaggccgggc atatcagccc cgacgacgat cgcgaattcc gtcgcgatct
     2401 cgcgcaaggt gccggcaagg gcggccagag ggcctgcgaa tggctcggcg tcgtcgatga
     2461 atcggacggg cggcacgtgg cggaacctcg tcgtcggttc gaccgggccg gcaatgagga
     2521 tggtgccggc gaccgattcg acgcgttcga ccgcacgctc caggagcgtc tggccatcgc
     2581 tggtcgccgc gagcttctcc gtcccgaagc ggctcgaccg gccgcccgcg aggacgatcc
     2641 cggtaacccg gtcgcgcccg actccggcg
//
LOCUS       DATBSW010000345         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_386128, whole genome shotgun sequence.
ACCESSION   DATBSW010000345 DATBSW010000000
VERSION     DATBSW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386128"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VIF63_09420"
     CDS             <1..385
                     /locus_tag="VIF63_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239642.1"
                     /translation="QIQEFLDYYLDSGTQHIALRTEDIVSTVRQLRENGIEFLGMPHA
                     YYETLADRVGDVGVPIDTLEELGIEADRDEEGYLLQIFTRPIQDRPTFFFEIIERHGS
                     RGFGVGNFKALFEAIEIEQARRGNL"
     gene            393..1034
                     /locus_tag="VIF63_09425"
     CDS             393..1034
                     /locus_tag="VIF63_09425"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEY8239643.1"
                     /translation="MARASGALIQAGTVGTALFVAVSTVLTATRAGYDPVRHFVSILS
                     LGDGGWVQVVNFVAGGLLMAGLGVGLGLRWRSRPGAGAIPRLVTIAGLGLVGCGVFIP
                     DPSLGYPPGTPDVLVTPLTWHGALHYACATVILVALTAAVLLAIRRRGTIGLRGLTAA
                     SIGVAATVLVGCGAALLLSGMPPTEIVGLTERIGIYAGWTWLVLLGLLELRSN"
     gene            1123..1548
                     /locus_tag="VIF63_09430"
     CDS             1123..1548
                     /locus_tag="VIF63_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8239644.1"
                     /translation="MAGKDSKLELLKTIPMFATCGTGDLERVAQLVDEVDIADGRVIM
                     RQGTPGNEMYIVATGMARAERDGRHLTDFGPGAVFGEMSIIAEGPRTATVTAVEPMRA
                     FVVGHREFHSLMDSYPDFKMRVLEGLAAKVRRLEENAVH"
     gene            1655..2287
                     /locus_tag="VIF63_09435"
     CDS             1655..2287
                     /locus_tag="VIF63_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8239645.1"
                     /translation="MTPRQYHLQKRAESTAATRQRVLDATIEVYREVGIPATTLKAVA
                     ERADVSRGTILHHFGSGEGLLGAVLDTILESLELPDVAAIEALDTLDARVRLFVGGMI
                     EFQDRTAHLWPIFEKELQRPQVAQREAAYWAALAKLQAATLGPGLRDDPAANAALLSV
                     IHPATVGTFQWAFGEAGLARADGGALLLEVAVDAVRRVADRSKRGKGGHQ"
     gene            2293..2676
                     /locus_tag="VIF63_09440"
     CDS             2293..2676
                     /locus_tag="VIF63_09440"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEY8239646.1"
                     /translation="MLRQLNAWLAWAFVAALFVQVWLAGSAIPQLGGAGSFATHREFG
                     YAIGLFSLILLVMAVISGTGRRRIGQAALLLVLYVVQSALPYMDPGLPAVAALHPVNA
                     LVMIGLAIWYARQVWRERGGTFATA"
BASE COUNT          400 a          907 c          960 g          439 t
ORIGIN      
        1 ccagatccag gagttcctcg actactacct cgactcgggc acgcagcaca tcgccctgcg
       61 cacggaggac atcgtctcga ccgtgcgcca gctgcgcgag aacggcatcg agttcctggg
      121 catgccccac gcctactacg aaacactcgc cgaccgcgtc ggcgacgtcg gcgtgccgat
      181 cgacacgctc gaggagctcg gcatcgaggc cgatcgcgac gaagagggct acctcctcca
      241 gatcttcacc cgcccgatcc aggatcggcc gacattcttc ttcgagatca tcgagcgcca
      301 cggctcacgg ggcttcggcg tcggcaactt caaggcgctc ttcgaggcga tcgagatcga
      361 acaagcccgc cggggcaatc tctgacccgc tcatggcgcg agcgtcgggc gcgctgatcc
      421 aggccggaac cgtcggcacc gccctgttcg tggcggtatc cacggtcctc accgcgacgc
      481 gggccggcta cgatcccgtc cggcacttcg tgagcatcct gagcctcggc gacggcggct
      541 gggtgcaggt cgtcaacttc gttgccggcg ggctgctgat ggctggcttg ggggtgggac
      601 tgggtctgcg gtggcgcagc cgccccgggg cgggcgccat cccacggctc gtgacgatcg
      661 ccggcctggg cctcgtgggc tgcggcgtct tcatccccga cccgtcgctc ggctacccgc
      721 cgggcacccc ggatgtgctc gtgacaccgc tcacctggca cggtgcgctg cactacgcct
      781 gcgcgacggt gatccttgtc gcgttgacgg cggcggtgct gcttgccatc cgccgcaggg
      841 ggacgatcgg gttgcgcggc ctgaccgccg cgtccatcgg cgtcgcggcg acagtgctcg
      901 tcggctgtgg cgccgcgctc ctgctgtcgg ggatgccacc caccgagatc gtcgggctga
      961 cggagcgaat cgggatctac gccggctgga cgtggctggt cctgctcggt ctgctcgagc
     1021 tgcggtcgaa ttgaatccgt tgctgacgcc tgaacgattc tcgcgaacgc gacgggacgg
     1081 ggtagtctct agcgcctgcg caggacgtcg agggaggtcg caatggccgg caaggattcg
     1141 aagctcgagc tgctgaagac gatcccgatg ttcgcgacgt gcgggaccgg cgacctggag
     1201 cgggtcgcgc agctcgtcga cgaggtcgac atcgcggatg gccgggtcat catgcggcag
     1261 ggcacgccgg gcaacgagat gtacatcgtc gcgaccggca tggcacgtgc cgagcgcgac
     1321 ggccggcacc tgaccgactt cggccccggt gcggtgttcg gcgagatgtc gatcatcgcg
     1381 gaagggccgc gcaccgcgac ggtcaccgcg gtcgagccga tgcgcgcgtt cgtggtgggt
     1441 caccgggagt tccattcgct catggattcg tatcccgact tcaagatgcg cgtccttgag
     1501 ggcttggcag cgaaggtccg gcgactcgag gagaacgcgg tccactaacc gagggcgtcg
     1561 tcgccgcagc atcgggccga cggcggacgg ccagcgttgg aaaatcgcgg gaacggtatt
     1621 gactgcgctg cgccgtcaat gaatactgcg cgtcgtgacc ccccggcagt accacctcca
     1681 gaagcgcgcc gagagcaccg ccgcaacgcg gcagcgcgtc ctcgacgcga ccatcgaggt
     1741 ctaccgcgag gtcggcatcc cggccaccac gctgaaagcc gtggcggagc gcgccgacgt
     1801 gtcgcggggc acgatcctcc accacttcgg atcgggggaa gggctgctgg gcgccgtcct
     1861 ggacacgatc ctcgaatccc tcgagctgcc cgacgtcgcg gcgatcgagg cgctcgacac
     1921 gctcgacgcg cgggttcggc tgttcgtcgg cggcatgatc gagttccagg accgaacggc
     1981 gcacctgtgg ccgatcttcg agaaggagct tcagcgaccc caggtcgcgc agcgcgaggc
     2041 cgcctattgg gcggcgcttg cgaagctcca ggccgccacg ctcggtcctg ggctgcgcga
     2101 tgacccggcg gcaaacgcgg cgctgctgag cgtcatccat ccggcgacgg tcggcacgtt
     2161 ccagtgggca ttcggggagg cgggcctggc ccgtgccgac ggcggggcgc tcctcctcga
     2221 ggtggcagtc gacgcggtcc gacgcgtggc agaccgcagc aagcggggca agggagggca
     2281 ccaatgagat cgatgctcag gcagctcaac gcctggttgg catgggcatt cgtcgcggca
     2341 ctgttcgtgc aggtctggct ggccggatcc gcgatcccgc agctcggcgg cgccgggtcg
     2401 ttcgcgaccc accgcgagtt cggctacgcg atcgggttgt tctcgctgat cctcctggtc
     2461 atggccgtca tctcgggcac cggccgccga cggatcggtc aggcggcgct gctcctcgtc
     2521 ctgtacgtcg tgcagtcggc gctgccgtac atggatccgg ggctgcccgc ggtggcggcc
     2581 cttcatccgg tcaatgcctt ggtcatgatc ggcctcgcga tctggtacgc ccgccaggtc
     2641 tggcgggaac gaggcgggac gttcgcgacg gcgtagggga tcgactcgaa gcgctgaagg
     2701 agggat
//
LOCUS       DATBSW010000346         5905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_174014, whole genome shotgun sequence.
ACCESSION   DATBSW010000346 DATBSW010000000
VERSION     DATBSW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174014"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /gene="aceE"
                     /locus_tag="VIF63_09445"
     CDS             complement(<1..1983)
                     /gene="aceE"
                     /locus_tag="VIF63_09445"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY8239647.1"
                     /translation="MTTTEPRSEMYIDEFKHQLPDIDEQETVDWIRSLDDVVDQEGET
                     RARFLLFKLLKRARQLQVGLPPLTNTRYINTISPEQEPEFPGDEGLERRIRRLIRWNA
                     VAMVLRANNKYSGIGGHLATYASAATLYEVGFNWFFKGRENGGPGDQVFYQGHAAPGI
                     YARAFLEGRLTTEQLDHFRRETPPQNGLSSYPHPRLMPDFWEFPTVSMGLGPIDAIYQ
                     ARFNRYLQNRGLVDTAGSRVWAFLGDGETDEPEALGALHVAADEGLDNLTFVVNCNLQ
                     RLDGPVRGNGKIIQELEAVFRGSGWNVIKVIWGREWDDLLARDRDGVLVEEMNATVDG
                     EFQKFSVMGGAYIREHFFGPDPRLQQLVEHLTDDDLAKLRRGGHDYRKVFAAYKAAIE
                     HRGAPTVILAKTVKGWTLGPGVEARNITHQAKKLSEQELRIFRDRLELPIPDEKLKDA
                     PYHHPGPESEEIKYLVERRRQLGGPLPRRVVRSKPLPAPAPAVDAEFAAGSQTPVSTT
                     MVFARILRNLIRDPELGPRIVPIIPDEARTFGMDPLFKEVGIYSALGQRYEPVDSDLV
                     LSYREATDGQVLEEGITEAGSMASFQAAGTSYASHGLATIPFYIFYSMFGFQRTGDQM
                     WAFGDARGRGFLMGATAGRTTLTGEGLQHDDGHSHVL"
     gene            complement(1980..2345)
                     /locus_tag="VIF63_09450"
     CDS             complement(1980..2345)
                     /locus_tag="VIF63_09450"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEY8239648.1"
                     /translation="MTFGESDRAALAAAQEIKIETQSATGTVHRTIIWVAEHDGQIYV
                     RSVNGPDARWYREAISGRPVAIHLGGRRLPVALEGATDPASIEACSEGLRAKYKRSYS
                     LRAMLAEDVLPTTLRAVPA"
     gene            2443..3075
                     /locus_tag="VIF63_09455"
     CDS             2443..3075
                     /locus_tag="VIF63_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY8239649.1"
                     /translation="MRRATLAAVRNRYDPTTALIVVDVQNDFADPLGSLFVTGAHDVV
                     RMVNGETRRATDDGALVVYSQDWHPDSTPHFAKDGGIWPVHCVGGSWGADFHPDLSVV
                     GPSVKKGVNGEDGYSAFTTKDPQTGATTPTELDALLRERGITRVVVVGLATDYCVSAT
                     ALDAARLGFETEVLQDAIAAVDLEPGDGDKARAAMAAAGCVLGYCFGTLP"
     gene            3117..4010
                     /locus_tag="VIF63_09460"
     CDS             3117..4010
                     /locus_tag="VIF63_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY8239650.1"
                     /translation="MRLHLVDATYELFRAHYAPRPPTLGRDGINLSGVSGIVDQLLFL
                     LREEGATHVGCATDRVIESFRNDMFPGYKSSAGMPPELLRQFPIAEAAIEALGLVLWP
                     MVEFEADDAIAAACVRFADDPALEQVVICTPDKDMAQLVRDSRVVLLDRRRRITYDEP
                     AVMEKWGVPPTAIPDWLALVGDSSDGYPGLPGWGAKSAAAVLARYGSLEEIPAKASAW
                     DVKSLRGGPVLAQTLRERWDEVQLYRSLARLRTTEDGVEIPQRDLDELRWRGTPRPVW
                     EAFCDEWGLDAQRARPHRWLD"
     gene            complement(4029..4847)
                     /locus_tag="VIF63_09465"
     CDS             complement(4029..4847)
                     /locus_tag="VIF63_09465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8239651.1"
                     /translation="MTVAPAVDRAADLAFATALARRAGDLLNGLFEKVERIDFKSAKD
                     VVTEADHMSEALIIEAIRAEYPTDGIIAEESGEHASGAGDAPSAGIGRAWVIDPLDGT
                     INYANGIPFFCVAIALVEAGRPVVGVIHDPIRGETFAASEDGPATLDGRLIHASTKDL
                     LSDYVVSMALSGRSLISRVRSVRSAVRVPRSMGSAALALAYVGNGRFDAFIQQGGLSL
                     WDIAAAGLIAERGGAAVTNLAGGDWFDVGHQPRSIGILSAPSAHHAELLRLVKG"
     gene            complement(4844..5332)
                     /locus_tag="VIF63_09470"
     CDS             complement(4844..5332)
                     /locus_tag="VIF63_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239652.1"
                     /translation="MTSAKGAVAPRRQLEPTHACIRCGREGVPMDAGLCELCNPLELS
                     QPSATQMHGIAAVGIIAFIVVLAVAGRVAIAGTGPFQGQVSAIVPAPAGLAVTILVTN
                     GGAKDAATTCAVTQSPTQNGPIVEVVQTPLIQAGTTLEYTAVVTKFGTQVVSLAVQCA
                     SP"
     gene            complement(5363..>5905)
                     /locus_tag="VIF63_09475"
     CDS             complement(5363..>5905)
                     /locus_tag="VIF63_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8239653.1"
                     /translation="RLILGMGIGGHPREHAAYGMDFPDAPRRIHHLEEAVATVRALWT
                     GGPVSRESDLYPLKEATAYPVPDPAPRIIVGGESRNGARLAGRIGDGWNAFDDNFEAN
                     LPTYLEALDEAGKQRADQLVLVGFQGEWLSDERIDETEWIREPAAARERWAAAGADGA
                     IVLARTTEDVDALVEARARW"
BASE COUNT          835 a         2049 c         2103 g          918 t
ORIGIN      
        1 gaggacgtgg gagtggccgt cgtcgtgttg gaggccctcg ccggtgaggg tcgtccgccc
       61 ggccgtggcg cccatcagga acccgcgccc gcgcgcatcg ccgaacgccc acatctggtc
      121 gccggtccgc tggaagccga acatcgaata gaagatgtag aacgggatgg tcgccaggcc
      181 gtggctggca tacgaggtcc cggcggcctg gaagctggcc atcgacccgg cctccgtgat
      241 gccctcctcc agcacctggc cgtcggtcgc ctcgcggtac gacaggacga ggtcggagtc
      301 gaccggttca tagcgctgcc ccaacgccga gtagatgccg acttccttga acagcgggtc
      361 catgccgaac gtccgcgcct cgtccgggat gatcggcacg atccgtggtc cgagctccgg
      421 atcgcggatg aggttgcgca ggatccgggc gaagaccatc gtcgtggaga ccggggtctg
      481 gctgccggct gcgaactcgg catcgaccgc cggcgcgggg gccgggagcg gcttggatcg
      541 cacgacacgg cgcggcagcg gtccaccgag ctggcggcgc cgctccacga ggtacttgat
      601 ctcctcggac tccggaccgg gatggtggta cggcgcgtcc ttgagcttct cgtcggggat
      661 cggcagctcc aggcggtcgc ggaagatccg gagctcctgc tcggacagct tcttcgcctg
      721 gtgggtgatg ttccgggcct ccacgccggg ccccagcgtc cagcccttca cggtcttggc
      781 gaggatcacg gtcggggcgc cgcgatgctc gatcgccgcc ttgtaggcgg cgaagacctt
      841 gcggtagtcg tggccgccgc ggcgcagctt ggcgaggtcg tcgtcggtca ggtgctcgac
      901 gagctgctgg aggcgcgggt caggcccgaa gaagtgctcg cggatgtacg cgccgcccat
      961 gaccgagaac ttctggaact cgccgtcgac cgtggcgttc atctcctcga ccaggacgcc
     1021 gtcccgatcg cgcgccagga ggtcgtccca ctcgcggccc cagatcacct tgatcacgtt
     1081 ccagcccgag ccgcggaaga ccgcctcgag ctcctggatg atcttgccgt tgccgcggac
     1141 cgggccgtcg aggcgctgga ggttgcagtt gacgacgaag gtcaggttgt cgaggccctc
     1201 gtcggcggcg acgtggagtg cgccgagcgc ctcgggctcg tccgtctcgc cgtcgccgag
     1261 gaatgcccac acacgactcc cggccgtgtc caccaggccg cggttctgga ggtagcggtt
     1321 gaaccgcgcc tgatagatcg cgtcgatcgg gccgaggccc atcgacaccg tcgggaactc
     1381 ccagaagtcg ggcatgaggc gcgggtgcgg gtatgacgac aggccgttct gtggcggcgt
     1441 ctcgcgccgg aagtggtcca actgctccgt ggtgaggcgc ccttcgagga acgcccgagc
     1501 gtagatgccc ggcgcggcgt ggccctggta gaagacctgg tcgccgggtc ccccgttctc
     1561 gcgacccttg aagaaccagt tgaacccgac ctcgtacagc gtggcggcgc tggcgtacgt
     1621 ggccaggtgc ccaccgatgc ccgagtactt gttgttggcc cgcagcacca tcgcgacggc
     1681 gttccaacgg atgagccggc ggatgcgccg ctcgaggccc tcatcgccgg ggaactcggg
     1741 ctcctgctcc gggctgatgg tgttgatgta gcgggtgttg gtgagcggcg gcagcccgac
     1801 ctggagctgg cgcgcccgct tcaggagctt gaacaacagg aatcgggccc gcgtctcgcc
     1861 ctcctggtcg acgacgtcgt cgagcgagcg gatccagtcg accgtctcct gctcgtcgat
     1921 gtcgggcagc tggtgcttga actcgtcgat gtacatctcg ctgcgcggct cggtcgtggt
     1981 catgcgggca ccgcgcgcag ggtggtcggc aggacatcct cggcgagcat cgcccggagc
     2041 gagtacgagc gcttgtactt ggcgcggagg ccctcgctgc acgcctcgat cgaagctggg
     2101 tcggtcgcgc cctcgagcgc cacgggcagg cgccggccgc cgaggtggat cgccacaggc
     2161 cgcccggaga tcgcctcgcg ataccagcgc gcatccgggc cattcaccga acgcacgtag
     2221 atctggccgt cgtgctcggc gacccagatg atcgtccggt ggaccgtccc ggtggcggac
     2281 tgtgtctcga tcttgatctc ctgcgctgcc gcgagcgcgg cgcgatcgga ttcaccgaat
     2341 gtcacccccg aacagtagcc gaagtcgcca tgcaaccggg gtcgcggctc ggttgcggcg
     2401 tggcggcaaa ttcgttcgca gacacacgag cgactccgcc gcatgcgccg cgctaccctc
     2461 gctgccgtgc gcaaccgcta cgacccgaca acggcgctga tcgtcgtcga cgtgcagaac
     2521 gacttcgccg atccactggg ctcactgttc gtcaccggcg cccacgacgt cgtgcgcatg
     2581 gtcaatggcg agacgcgccg cgcaaccgat gacggcgcgc tggtcgtgta ctcgcaggac
     2641 tggcatcccg attcgacgcc gcacttcgcc aaggacggcg gcatctggcc ggtccattgc
     2701 gtcggcggct cgtggggcgc cgacttccac cccgacctca gcgtcgtcgg gccgtcggtg
     2761 aagaagggcg tgaacggcga ggacggctac tcggccttca cgacgaagga cccgcagacg
     2821 ggcgcaacga cgccgaccga gctggacgcc ctgctccgtg agcgcgggat cacgcgcgtc
     2881 gtcgtggtcg gcctggccac ggactactgc gtcagcgcga ccgccctcga cgcggcgcgg
     2941 ctcgggttcg agaccgaagt cctgcaggac gcgatcgcgg ccgtggacct ggagcccggc
     3001 gacggcgaca aggcgcgcgc ggcgatggcc gctgccggct gcgtgctcgg gtactgcttc
     3061 ggaacgctgc cctagcccgt tccttgccct cgcgcgatcg gggctacagt ccaccgatgc
     3121 gcctccacct cgtcgacgcg acgtacgagc tgttccgggc ccactacgcg ccgcgaccgc
     3181 cgaccctcgg gcgcgacggc atcaatctct cgggtgtctc gggcatcgtc gaccagctgc
     3241 tgttcctcct gcgcgaggag ggcgcgaccc atgtcggctg cgcgacggac cgggtgatcg
     3301 agtcgttccg gaacgacatg ttccccggct acaagtcgtc ggccgggatg ccgccggagc
     3361 tcctgcgcca gttcccgatc gccgaggcgg ccatcgaggc actgggtctg gtgttgtggc
     3421 cgatggtcga gttcgaggcg gacgacgcga tcgccgccgc gtgcgtgcga ttcgcggatg
     3481 acccggcgct cgagcaggtc gtcatctgca cgccggacaa ggacatggcg cagctcgtcc
     3541 gcgactcgcg cgtcgtgctc ctggaccggc gccggcgcat cacctacgac gagccagccg
     3601 tgatggagaa gtggggcgtg ccgccgaccg ccatccccga ctggctcgcg ctcgtcggtg
     3661 actcatcgga cggctacccc ggtctgcccg gctggggcgc gaagtccgct gcggcggtcc
     3721 tggcgcgata cggctcgctc gaggagatcc ccgcgaaggc gtcagcgtgg gacgtgaaga
     3781 gcctgcgcgg cggcccggtc ctcgcccaga ccctgcgcga gcgctgggac gaggtgcagc
     3841 tctaccgctc cctcgcccgc ctgcgcacga ccgaggacgg cgtcgagatc ccgcagcgcg
     3901 acctcgatga gctgcgctgg cgcggcacgc cgcggcccgt ttgggaggcg ttctgcgacg
     3961 aatggggcct cgacgcccag cgcgcccgac cgcatcgctg gctggactga tccggcaggg
     4021 ccggttggtc agcccttcac gaggcggagc agctcggcgt ggtgggcgct cggcgcgctg
     4081 aggatcccga tcgagcgcgg ctggtggccg acgtcgaacc agtcgccacc cgcgagattt
     4141 gtcacggccg cgccgccgcg ctcggcgatg aggccggcgg ccgcgatgtc ccagagcgac
     4201 aggccgccct gctggatgaa cgcgtcgaag cggccgttgc cgacgtaggc gagggcgagc
     4261 gccgccgagc ccatcgagcg cggcacgcgt accgcactgc ggaccgatcg gacgcgcgag
     4321 atcagcgacc gacccgagag cgccatcgac acgacatagt cggacaggag gtccttggtc
     4381 gaggcgtgga tcaggcgccc gtcgagggtc gccgggccgt cctcgctggc ggcgaatgtc
     4441 tcgccccgga tcgggtcgtg gatcaccccg acgaccggcc ggcccgcttc gacaagcgcg
     4501 atcgcgacgc agaagaacgg gatgccgttg gcgtagttga tcgtgccgtc caggggatca
     4561 atgacccacg cccgcccgat gccggcgctc ggcgcgtcgc cggcaccgct ggcgtgctcg
     4621 ccgctctcct cggcgatgat tccgtcggtt gggtattcgg cgcggatggc ctcgatgatc
     4681 agtgcctcgg acatgtggtc ggcctcggtg acgacgtcct tggccgactt gaagtcgatg
     4741 cgctccacct tctcgaagag gccattcagc aggtcgccgg cgcgccgcgc cagtgcggtt
     4801 gcgaacgcga gatccgcagc tcgatcgacg gcgggcgcga cggtcacggc gacgcgcact
     4861 gcacggccag cgagacgacc tgcgtgccga acttcgtcac caccgcggtg tactccagcg
     4921 tcgtgccggc ctggatcagc ggcgtctgca cgacctcgac gatcggtccg ttctgggtcg
     4981 gactctgggt caccgcgcag gtcgtggccg cgtccttcgc gccgccgttg gtcacgagaa
     5041 tcgtcactgc aagcccggca ggcgccggca cgatcgcgct cacctggccc tggaacgggc
     5101 ccgtgccggc gatcgcgacc cggcccgcca cggcgagcac gacgatgaac gcgatgatcc
     5161 cgaccgcggc gatgccgtgc atctgcgtcg ccgacggctg cgacagctcg agcgggttgc
     5221 acagctcgca cagcccggcg tccatcggca cgccctcgcg accgcatcga atgcaggcat
     5281 gtgtcggttc gagctggcgg cgcggggcga cggcgccttt cgcggacgtc atcggcactg
     5341 ctcgcgagtg gcaatcatcg gatcaccacc gggcgcgcgc ttcgacgagc gcatcgacat
     5401 cctcggtggt gcgggcgagg acgatcgcgc cgtcggcgcc ggcggcggcc cagcgctcgc
     5461 gtgcggccgc gggctcgcgg atccattcgg tctcgtcgat tcgctcgtcg gacagccact
     5521 cgccctggaa gccgacgagc acgagctggt cggcgcgctg cttccccgcc tcgtcgagcg
     5581 cctcgaggta cgtgggcagg ttcgcctcga agttgtcgtc gaaggcgttc cagccatcgc
     5641 cgatccggcc ggcgagccgc gcgccgttgc gactctcgcc gccgacgatg attcgcggcg
     5701 cgggatccgg cacgggatac gcggtggcct ccttgagcgg gtacaggtcc gactcgcggc
     5761 tcaccggccc gccggtccac agcgcgcgga ctgtcgcgac cgcctcctcg aggtggtgga
     5821 tccgccgtgg cgcgtcgggg aagtccatgc cgtatgcggc gtgttctcgg gggtggccgc
     5881 cgatgcccat gcccaggatc agccg
//
LOCUS       DATBSW010000347         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_773829, whole genome shotgun sequence.
ACCESSION   DATBSW010000347 DATBSW010000000
VERSION     DATBSW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773829"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..957
                     /locus_tag="VIF63_09480"
     CDS             10..957
                     /locus_tag="VIF63_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8239654.1"
                     /translation="MTLDQTSEPAPPKERPAFLPSSIDPTVFDEGFLRQLERLLLLMK
                     RPVRGGLKGGRRSVKRGQSVEFADFRDYSLGDDLRQLDWNVLARLEKLFIKLYVEEED
                     VTISFLLDCSASMAAGQPPKLLFAKRAAAALGYIGLASEDRVAVTALAARAGRRQVAL
                     RGSGRVFRLLSALSNIQSADGPTDLLAAARHAGAQLSGRGVIVLISDLLDPAADKVIR
                     ELAATGSELIILHVLSPDELDPSMEGDVRLVDVETGDGLDVTIDLATIDDYKARLAAW
                     QEGFADLASRRGATYVPIATDLPLADLVFAELRRRRVLG"
     gene            957..3152
                     /locus_tag="VIF63_09485"
     CDS             957..3152
                     /locus_tag="VIF63_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8239655.1"
                     /translation="MGFLSPLALLGLLFLPVVVAMYLLKLRRDERVVPSTLLWQKLLT
                     DVEANAPWQRLRKSLLLLLQLLLVALLAILAARPFLGRPAGLAGDVVIIIDTSASMAA
                     SDVPPSRLEEAKHQVLEALHELPTNGTVSVIAAGRTARVVINGTTDLGRVRAAIEEIK
                     VSPATGDLGDALNLANALAARAGDADILVATDAALSIKPTTRVDHEVKVIQVGRERKN
                     QAIVALAVRHSSSGVTRSVFVSIANLDIESAARRVELYSNGILIEARPLPNLEPQTRT
                     EVIIDDLPPEATNIEVRLVALNGAVDGVDPDALTVDDRAWAVIPPDRLREILIVGEGD
                     PYLETALTYLPNATLFGVKPADFPARATRTDGRPWDLIVFEGVVPSHPELSASVLAIA
                     PPATSALGEVVGTLTDPGIGTPSTDEPILKFVDLSSVHIGKATKLVAPEWARTVIRGP
                     AGSPLLYIGELDGRRSAVLAFLPRNSDLPLQVAFPLLIANLSGELLGGSAAPTEALAP
                     GDPVTLGVPSGATSVVVTRPDGSTVELVPATAGAASVAFSQTDLLGIYSVAATYPNAT
                     PGPSGGLPSGGLPSVLATPRPSGSTPSASLAPGASPTPVPTAPPVNPNAPVQFAVDLF
                     DPGESNIAPGSPSDIASLGRQPGASVAPGASGGPTAGPTLEPGTSPEPGSSPVAGAGT
                     GEERPIARDELWVLVVLIVLAVLMAEWLVYHRDAVTRLWRGLRRTPGAG"
     gene            3152..>4498
                     /locus_tag="VIF63_09490"
     CDS             3152..>4498
                     /locus_tag="VIF63_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8239656.1"
                     /translation="MGIRFEAAIWLVLLIPALLLTFIPHLAARRRMGARRRRAAVAIR
                     AILLTALVLALAGFQLILPVDRLATVFVVDLSDSVGESGRQDALAFLRETLEVMPEED
                     QAGIVAFGKDALVERLPEELRAIDRIRSTPLKAATDIGAALRLAAALFPDAAQKRIVL
                     ISDGNDTTGRGQREAAFAASRGIQVETRVIGLVDREEVLVERLTTPSTARLGEEIEAI
                     ADISSSVAQTATVRLFADGVLVATNRVDLKAGPNRIVFRVKPSEPGFHTFRAVVEAAG
                     DTFSQNDRADSNTIIKGEPRILVLAGDQAVAAELVAALKAEKQLVDSMIPEALPTDFA
                     GLVSYDSIVVVDVPRLRFNDRQLAALQVYVRDLGRGLVMVGGPRAYGAGGYTKTAMEE
                     LLPVDMGVRDRNKEPDIALVVVIDKSGSMDACHCNTFDGGMGGGGAMSGVRKVDIGK"
BASE COUNT          616 a         1640 c         1552 g          690 t
ORIGIN      
        1 gcctaattca tgaccctcga tcagacctcc gaaccggcgc cgcccaagga gcgcccggcg
       61 ttccttcctt cgagcatcga cccgaccgtc ttcgatgagg gattccttcg gcaattggag
      121 cggctgctcc tgctcatgaa gcggcccgtc cggggcgggc tcaagggcgg gcgacgctcg
      181 gtcaagcgcg gccaatcggt cgagttcgcc gacttccgtg actactcgct cggcgacgac
      241 ctgcgccagc tcgactggaa cgtcctcgcc cgcctcgaga agctgttcat caagctctac
      301 gtcgaggaag aggacgtcac catctccttc ctcctcgact gctcggcatc gatggcggcc
      361 gggcagccgc cgaagctcct gttcgccaag cgggccgctg ccgcgctcgg ctacatcggg
      421 ctggcctcgg aggatcgcgt cgccgtcacg gccctcgcgg cgcgggccgg gcggcgccag
      481 gtggccctgc gcgggtccgg tcgcgtgttc cggctgctct cggcgctgtc gaacatccag
      541 tcggccgatg gcccgaccga cctcctcgcc gccgcgcgcc atgccggcgc ccagctgagc
      601 ggccgcggcg tcatcgtgct gatctcggac ctgctcgatc ccgcggccga caaggtcatc
      661 cgcgagctgg ccgcgaccgg ctccgagctg atcatcctcc acgtcctgtc gcccgacgag
      721 ctcgacccgt cgatggaggg cgacgtccgg ctcgtcgacg tcgagaccgg cgatgggctc
      781 gacgtcacca tcgatctcgc gacgatcgat gactacaagg cccggctcgc ggcgtggcag
      841 gagggtttcg ccgacctcgc ctcgcgccgc ggtgcgacgt acgtgccgat cgcgacggac
      901 cttccgctcg ctgacctcgt gttcgcggaa ttgcgccggc ggcgggtgct gggctgatgg
      961 gcttcctgtc gccgctcgcg ctgctcggcc tgctgttcct gccggtcgtg gtcgcgatgt
     1021 acctgctcaa gctccggcgc gacgagcggg tcgtgccctc gacgctgctc tggcagaagc
     1081 tgctcaccga tgtggaggcc aacgcgccgt ggcagcggct gcggaagagc ctcctgctgc
     1141 tgctccagct gctcctcgtc gcgctgctgg cgatcctcgc tgcccgcccg ttcctggggc
     1201 gcccggcggg actcgcgggc gatgtcgtga tcatcatcga cacctccgcg agcatggccg
     1261 ccagcgatgt gccgccgagc cggctcgaag aggcgaagca ccaggtcctc gaggcgctcc
     1321 acgagctgcc cacgaacggg accgtgagcg tgatcgcggc gggtcgcacc gcccgcgtcg
     1381 tgatcaacgg cacgaccgac ctcggccgag tgcgggcggc aatcgaggag atcaaggtct
     1441 cgcccgcgac cggcgacctc ggcgacgcgc tgaacctcgc gaacgccctg gcggctcggg
     1501 ccggggatgc ggacatcctc gtggccaccg acgcggcgct ctcgatcaag ccgacaacgc
     1561 gggtcgacca cgaggtgaag gtcatccagg tcgggcggga gcggaagaac caggcgatcg
     1621 ttgccctcgc cgtgcgtcat tcctcgtccg gcgtgacccg ctcggtgttc gtgagcatcg
     1681 ccaacctcga catcgagtcg gctgcccgac gggtcgagct gtattccaac ggcatcctga
     1741 tcgaggcacg gccccttccg aacctcgagc cgcagacgcg gaccgaggtc atcatcgacg
     1801 acctgccgcc cgaggccacg aacatcgagg tacggctcgt cgcgctgaac ggcgcggtgg
     1861 atggcgtcga tcccgacgcg ctgaccgtcg acgatcgcgc gtgggccgtc atcccgcccg
     1921 accgactgcg cgagatcctc atcgtcggcg agggcgaccc gtatctcgag accgcgctga
     1981 cctatctgcc gaacgcgacg ttgttcgggg taaaacccgc ggactttccg gcaagggcga
     2041 cccgcactga cggccggccg tgggatctga tcgtgttcga aggtgtcgtc ccgtcgcatc
     2101 ccgaactgtc ggcttccgtg cttgcgatcg cgccaccggc gaccagcgcc ctgggtgagg
     2161 tcgtgggcac gctgaccgac ccgggcatcg ggacgccatc gaccgacgag ccgatcctca
     2221 agttcgtcga cctctcgagc gtgcacatcg gcaaggccac gaagctcgtg gcgcccgagt
     2281 gggcgagaac cgtgatccgc gggccggcag gatctccgct cctctacatc ggcgagctcg
     2341 acggccgccg ttcggcggtg ctcgcgttcc tgcctcgcaa ctccgacctg cccctgcagg
     2401 tcgccttccc gttgctgatc gccaacctga gcggcgagct cctgggtggt tcagcggctc
     2461 cgaccgaggc gctcgcgccg ggtgaccccg tcacgctggg ggtgccgtcg ggcgcgacct
     2521 cggtcgtcgt cacgcggccg gacgggtcga cggtcgagct cgtgcccgcg acggccggcg
     2581 cggcgtcggt cgcgttcagc cagaccgacc tgctcggcat ctactcggtc gccgcgacgt
     2641 atccgaatgc gacgccggga ccctcgggtg gacttccgtc cggcggcctg ccgtcggtgc
     2701 tcgcgacgcc gcggccgtcc gggtccaccc cgtcggcaag cctggcgccc ggtgcctccc
     2761 ccaccccggt cccgaccgcg ccgccggtga acccgaacgc gccggtgcag ttcgcggtcg
     2821 acctgttcga cccgggcgag agcaacatcg cgcccggatc gccgtcggac atcgcgtcgc
     2881 tcgggcgaca acccggggcc tccgtggcgc cgggcgcgtc gggcggcccg acagctggcc
     2941 cgaccctgga gcccggcaca tcacccgagc ctggctcatc cccggtcgcg ggcgccggga
     3001 ccggtgagga gcgcccgatc gcccgtgacg agctgtgggt gctcgtcgtc ctcatcgtcc
     3061 tcgccgtgct gatggccgag tggctcgtgt accaccgaga cgcggtgacg cgcctgtggc
     3121 gcgggctgcg gcgaacgccg ggcgccggct gatgggcatc cgcttcgagg ccgcgatctg
     3181 gctggtgctg ctcatcccag cattgctgct gacgttcatc ccgcacctcg ccgcgcgccg
     3241 gcggatgggc gcgcgccggc gccgcgcggc ggtcgccatc cgcgcgatcc tgctgacggc
     3301 gctcgtcctc gcgctggccg gattccagct catcctgccc gtcgatcgcc tcgccacggt
     3361 gttcgtcgtc gacctgtccg acagcgtcgg cgagagcggc cggcaggacg cgctggcctt
     3421 cctgcgcgag accctcgagg tcatgcccga ggaggaccag gcgggcatcg tcgcgttcgg
     3481 caaggacgcg ctggtcgagc ggctacccga ggagctgcgc gccatcgacc ggatccggag
     3541 cacgccgctc aaggcggcca cggacatcgg cgctgcgctc cggctcgccg ccgcgctgtt
     3601 ccccgacgcg gcgcagaagc ggatcgtgct catctcggac ggcaacgaca cgaccgggcg
     3661 cgggcaacgc gaggccgcgt tcgccgcgtc gcgcgggatc caggtcgaga cgcgggtcat
     3721 cgggctggtc gaccgcgagg aggtgctcgt cgaacggttg acgacgccat cgaccgcccg
     3781 gctcggcgag gagatcgagg cgattgccga catcagctcg agcgtcgcgc agaccgcaac
     3841 cgtgcgcctc ttcgccgacg gcgtgctcgt cgcgaccaat cgggtcgacc tcaaggccgg
     3901 cccgaacagg atcgtcttcc gggtcaagcc atcggagccc gggttccaca ccttccgggc
     3961 ggtcgtcgag gcggcgggtg acacgttcag ccagaacgat cgcgccgact cgaacacgat
     4021 catcaagggc gagccgcgga ttctcgtgct tgcgggcgac caggccgtcg cggcggagct
     4081 cgtcgccgcc ctcaaggcgg agaagcagct cgtcgactcg atgatccccg aggcgctgcc
     4141 gacggacttc gcgggcctcg tctcgtacga cagcatcgtc gtcgtcgacg tgccgcggct
     4201 gcgcttcaac gaccgccagc tcgctgcgct ccaggtctac gtccgcgacc tcggccgcgg
     4261 gctcgtcatg gtcggtgggc cgcgcgcata cggagcgggt ggctacacga agaccgcgat
     4321 ggaggagctc ctcccggtcg acatgggcgt ccgcgaccgg aacaaggagc cggacatcgc
     4381 cctcgtcgtc gtcatcgaca agtcgggctc gatggacgcc tgccactgca acaccttcga
     4441 cggcggcatg ggcggcggtg gcgcgatgtc cggcgtgcgc aaggtcgaca tcggcaag
//
LOCUS       DATBSW010000348         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1020541, whole genome shotgun sequence.
ACCESSION   DATBSW010000348 DATBSW010000000
VERSION     DATBSW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020541"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1844)
                     /locus_tag="VIF63_09495"
     CDS             complement(150..1844)
                     /locus_tag="VIF63_09495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY8239657.1"
                     /translation="MPKRTPPPAPDAGDTPLRIIPLGGVGEIGKNMYVIEYGDDIVVI
                     DCGLMFPDEEMFGIDLVIPDVTYLRERKANVKAFLITHAHEDHVGGLPYILPNFPGVP
                     VYASTLARGLLGIKIKEHKLTSNPLLPLDPGDELQVGPFTVIPFRVGHSIPDAMGIAL
                     RTPVGTIVHTGDFKFDHTPVDGKLSDFAIIAKLGAEGVICLLSDSTRAESPGYTPSER
                     TVGEAFREIMEPLDGRVIVATFASNIARIQQVLDAAATFDRKVAVIGRSMEQNFRIAA
                     DLGYLTFAPDRMVAKDRIGDVQDGKIVIATTGSQGEPMSGLARMANRDHRFVEIEQGD
                     TVILSASPIPGNEEYVSRTIDNLFKAGANVIYHAIRHAHVSGHASQEELKLMLNLVRP
                     RYFIPMHGEFRMQVHHGRLAVETGVSPENVFIIENGTPIEFWADGSARRGQPVPAGYL
                     FVDGLSVGEVGEFVLRDRRALANDGMFMIVVTVDKQTGAVLGKPEIITRGFVHGNEDD
                     PIIEEAILRVMDAVKNPGEHISEIALLKSQIKDGVSRFLFEKTKRRPMVFPVVVEV"
     gene            complement(1844..>2685)
                     /locus_tag="VIF63_09500"
     CDS             complement(1844..>2685)
                     /locus_tag="VIF63_09500"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8239658.1"
                     /translation="DGAHRNDHGRDARVIAAGLAASEGAPSSGSLVSTEARRAALEAI
                     AAEVRTCTRCRLAQTRTRAVPGEGDPNTEVVFVGEGPGQTEDQQGRPFVGRAGDLLVK
                     LLSTLGWRRNEVFITNIVKCRPPGNRDPELDEIAACAPYLQRQLDVIDPAVVATLGRF
                     SMAHFRPGERITQIHGTAVAAPPETGARDALAFALFHPAAALRSTDVERQSYDDIARL
                     PRVLLDARTRRGGAGASAGAAKPAPAAAPDTGATAAQPALPTDQALVLELPAEPTMFD
                     TEA"
BASE COUNT          384 a          884 c          978 g          439 t
ORIGIN      
        1 agcgtcattg caccgaggac gaggatcgcc atcccgacga gcgatcgggc gacgtccttg
       61 ccgacgtcgg gcaggcggaa cgacgggagc ccgagggacc cccgcttgcg gcgcgcctgg
      121 cgtgtcgggc gccgtcgacg ggtcgccacc tacacctcca ccacgaccgg gaagaccatc
      181 ggccggcgct tcgtcttctc gaacaggaag cgcgagacgc cgtccttgat ctggctcttc
      241 aggagggcga tctcgctgat gtgctcgccc gggttcttga cggcgtccat gacccgaagg
      301 atggcctcct cgatgatcgg gtcatcctcg ttgccgtgga cgaaaccgcg ggtgatgatc
      361 tcgggcttgc cgaggacggc gccggtctgc ttgtcgaccg tgacgacgat catgaacatg
      421 ccgtcgttcg cgagggcgcg ccggtcacgc aggacgaact cgccgacctc accgacggac
      481 agcccgtcga cgaacaggta gcccgccgga accggctgcc cgcgccgggc agagccatcc
      541 gcccagaact cgatcggcgt cccgttctcg atgatgaaca cgttctccgg cgacaccccc
      601 gtctcgaccg cgagccgtcc atggtgcacc tgcatccgga attcgccgtg catcgggatg
      661 aagtaccgcg gccggaccag gttgagcatc agcttgagct cctcctggct cgcgtggccg
      721 gacacgtggg cgtgccggat cgcgtggtag atgacattgg cgccggcctt gaacaggttg
      781 tcgatcgtgc gcgagacgta ctcctcgttg cccgggatcg ggctcgccga caggatcacc
      841 gtgtcgccct gctcgatttc gacgaagcgg tggtcgcggt tggccatgcg cgcgaggccg
      901 gacatcggct cgccctggga gccggtcgtc gcgatgacga tcttcccgtc ctggacgtcg
      961 ccgatgcggt ccttggcgac catcctgtcg ggggcgaagg tgaggtagcc gaggtcggcg
     1021 gcgatccgga agttctgctc catcgatcgc ccgatgaccg cgaccttgcg gtcgaacgtc
     1081 gcggcggcgt cgagcacctg ctggatgcgc gcgatgttgc tcgcgaacgt ggccacgatg
     1141 acccggccgt cgagcggctc catgatctcg cggaatgcct cgccgaccgt ccgctcggac
     1201 ggcgtgtagc cggggctctc tgcgcgcgtc gagtccgaga gcaggcagat cacgccctcg
     1261 gcgccgagct tggcgatgat cgcgaagtcg gacagcttgc cgtcgaccgg ggtgtggtcg
     1321 aacttgaagt cgccggtgtg gacgatggtg ccgaccgggg tgcggagggc gatgcccatc
     1381 gcatcgggga tcgagtggcc gacccggaac gggatgaccg tgaacggccc gacctggagc
     1441 tcgtcgccgg ggtcgagcgg gaggagcggg ttcgaggtga gcttgtgctc cttgatcttg
     1501 atcccgagca ggccgcgggc gagggtcgag gcgtacaccg gcacgcccgg gaagttcggc
     1561 aggatgtatg gcaggccgcc gacgtggtcc tcgtgggcgt gggtgatgag gaacgccttg
     1621 acgttggcct tgcgctcgcg caggtacgtc acgtcgggga tgacgaggtc gatgccgaac
     1681 atctcctcgt ccgggaacat caggccgcaa tcgatcacga cgatgtcgtc gccgtactcg
     1741 atcacgtaca tgttcttgcc gatctccccc accccgccca gcgggatgat ccggagcggc
     1801 gtgtcacccg cgtccggggc cggtggtggg gtgcgctttg gcatcaggcc tccgtgtcga
     1861 acatggtggg ttccgcgggg agctccagca cgagggcctg atccgtcggc aaggcgggct
     1921 gggcggcagt ggcgccggtg tcaggggcgg cggccggtgc gggcttcgca gcgcctgcgc
     1981 ttgcgccggc tcccccacgg cgggtcctgg catccaggag cacgcgcggc agccgggcaa
     2041 tgtcgtcgta gctctggcgc tcgacatcgg tgctgcggag cgcagccgcg ggatggaaga
     2101 gggcgaacgc gagcgcgtcc cgggcgccgg tctcgggcgg ggcggccacg gcggtcccat
     2161 ggatctgggt gatccgctcg ccgggccgga agtgggccat cgagaacctg cccagcgtcg
     2221 cgaccaccgc gggatcgatc acgtcgagct ggcgctggag atacggcgcg caggccgcga
     2281 tctcgtctag ctcggggtcg cgattgccgg gcggccggca cttgacgatg ttcgtgatga
     2341 acacctcgtt gcgccgccag ccgagcgtgg acagcagctt cacgagcagg tcgccggcgc
     2401 ggccaacgaa cggccggccc tgctggtcct ctgtctggcc ggggccctca ccgacgaaca
     2461 cgacctcggt gttcgggtcg ccttcaccgg gcacggcgcg cgtcctggtc tgggcgaggc
     2521 ggcaacgcgt gcaggtgcgc acctccgcgg caatcgcctc gagggcggcg cgtcgtgctt
     2581 cggtcgacac gaggcttccg ctcgacggtg cgccctcgct cgcagcgagc cccgccgcga
     2641 tcacgcgcgc gtctcgaccg tggtcgttcc gatgggctcc gtcgc
//
LOCUS       DATBSW010000349         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_844479, whole genome shotgun sequence.
ACCESSION   DATBSW010000349 DATBSW010000000
VERSION     DATBSW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844479"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="hppD"
                     /locus_tag="VIF63_09505"
     CDS             complement(<1..926)
                     /gene="hppD"
                     /locus_tag="VIF63_09505"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY8239659.1"
                     /translation="MTDTISRGAANQAGAILNKDAYKEAKDFLPLNGVDHVEFWVGNA
                     KQAAHYYRALWGFTPIAYAGLETGVRDRISYVMRQNDITIVVTGALSPDGEIAEHVHR
                     HGDGVKDIAFTVDDATASWRETTTRGAKSAIEPVELDGGEDGVLRRSAVGTYGEVRHS
                     FIDRRDYHGVFAPGYRRVKNPARAASGMNLLEIDHCVGNVWLGDMNKFVDFYRDVFGF
                     AQLIHYDDKVIHTEYSALMSKVMTNGNGRVKFPINEPAPAKKKSQIQEFLDYYLDSGT
                     QHIALRTEDIVSTVRQLRENGIEFLGMPHAYY"
     gene            complement(923..1465)
                     /locus_tag="VIF63_09510"
     CDS             complement(923..1465)
                     /locus_tag="VIF63_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239660.1"
                     /translation="MERPRALSPLPATGRAARRARQQAIRDLVAEDQIGSQQQLADRL
                     AELGFDVTQATVSRDIAELGLVKIARDDRHVYASAATLTALPRSDDSMLRRVLGDLQI
                     DIRRSGLTLLLVSTPGTASILAEAIDRSTFNDQVGTLAGDNTVLVLFGDEPALDRWRD
                     RMLDLQATVITTLARDGGRP"
     gene            complement(1473..>2226)
                     /locus_tag="VIF63_09515"
     CDS             complement(1473..>2226)
                     /locus_tag="VIF63_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY8239661.1"
                     /translation="ANSIDSVGDRDFIVEVLAAVALCQVHLSRLAEEIVWWSNPRFGF
                     VRLAEAFSTGSSMLPNKRNPDPAELVRGRAARVIGDLAGFLALLKGLPLGYQRDLQED
                     KPPLFEACRILGSSLEVMAGVVGGLEVDERRLADAVAEGFTTAVALADRLVDHGVAFR
                     AAHHVVGAFVRAAEDRGVGLEALTDDVIAAALAASDDDAARRLATDPGIAADLRRQAV
                     PGAAVERADVVGGTAPARVAAELAEAARRLGV"
BASE COUNT          321 a          729 c          820 g          356 t
ORIGIN      
        1 tcgtagtagg cgtggggcat gcccaggaac tcgatgccgt tctcgcgcag ctggcgcacg
       61 gtcgagacga tgtcctccgt gcgcagggcg atgtgctgcg tgcccgagtc gaggtagtag
      121 tcgaggaact cctggatctg gctcttcttc ttggccggcg ccggctcgtt gatcgggaac
      181 ttgacccggc cgttgccatt ggtcatgacc ttcgacatca gggccgagta ctcggtgtgg
      241 atgaccttgt cgtcgtaatg gatgagctgg gcaaagccga agacgtcgcg atagaagtcg
      301 acgaacttgt tcatgtcgcc gagccacacg ttgccgacgc agtggtcgat ctcgaggagg
      361 ttcatgccgg acgcggccct ggccgggttc ttcacgcgcc gatagcccgg cgcgaagacg
      421 ccgtggtagt cgcgccgatc gatgaacgag tggcggacct cgccgtacgt gccgaccgcg
      481 ctgcggcgca ggacgccgtc ctcgccgccg tcgagctcga cgggctcgat cgccgacttc
      541 gcgccgcgag tcgtcgtctc gcgccatgag gccgtcgcgt cgtcgacggt gaacgcgatg
      601 tccttcaccc catcgccgtg ccggtggacg tgctcggcga tctcgccgtc ggggctgagc
      661 gcgccggtga cgacgatcgt gatgtcgttc tggcgcatga cgtagctgat ccggtcgcgg
      721 acgccggtct cgaggcccgc atacgcgatg ggcgtgaagc cccacagcgc ccggtagtag
      781 tgcgccgcct gcttggcgtt gccgacccag aactcgacgt ggtcgacgcc gttcagcggc
      841 aggaaatcct tcgcttcctt gtaggcgtcc ttgttgagga tggcgccggc ctggttcgcg
      901 gcgccgcggg agatcgtgtc ggtcatggcc ggcctccatc tcgggcgagg gtggtgatca
      961 cggttgcctg caggtccagc attcgatcgc gccaacgatc gagcgccggc tcgtcaccga
     1021 acaggacgag gacggtgttg tcgccggcga gggtgccgac ctggtcgttg aacgtggagc
     1081 ggtcgatcgc ttcggcgagg atcgacgccg tccccggtgt cgagaccagg agcagcgtga
     1141 gcccggatcg gcggatgtcg atctggaggt cgcccaggac gcgccggagc atcgaatcgt
     1201 cgctccgtgg cagggcggtg agggtcgccg ccgaggcata gacgtgtcgg tcgtcccggg
     1261 cgatcttgac gagcccgagc tcggcgatgt cgcggctgac ggtcgcctgg gtaacgtcga
     1321 agccgagctc cgcgagccgg tcggcgagct gctgctggct gccgatctgg tcctccgcga
     1381 cgaggtcccg aatggcctgc tggcgggctc gccgggcagc gcgtccggtg gcgggcaacg
     1441 ggctgagcgc gcgaggtcgc tccatcggcc gactatacgc cgagccggcg ggcggcctcc
     1501 gcgagctccg ctgcgaccct cgccggagcc gtgccgccga cgacatcggc acgctcgacg
     1561 gcggcgcccg gaacagcctg ccggcgcagg tcggcggcga tccccggatc cgtggcgagc
     1621 ctgcgcgctg cgtcgtcgtc gcttgctgcg agggcggccg cgatgacgtc atcggtgagc
     1681 gcctccagcc cgacgccgcg gtcctccgcg gcccggacga aggcgccgac gacgtggtgg
     1741 gcggcgcgga aggcgacgcc gtggtcgacg agccgatcgg cgagggcgac ggcggtcgtg
     1801 aagccttcgg caacggcgtc ggcgaggcgt cgctcatcga cctccaaccc tccgacgacg
     1861 ccggccatga cctcgaggga gctgcccagg atccggcagg cctcgaacag cggtggcttg
     1921 tcctcctgca ggtcgcgctg gtacccgagc ggcaggccct tgagcagcgc gaggaagccg
     1981 gcgaggtcac cgatgacccg tgctgcccgg ccccgaacga gctcggccgg gtcgggattg
     2041 cgcttgttgg ggagcatcga cgagcccgtc gagaacgcct cggcgagacg gacgaagccg
     2101 aagcgcgggt tcgaccacca gacgatctcc tcggccagcc gcgacaggtg gacctggcag
     2161 agcgcgacgg ccgcgaggac ttcgacgatg aagtcccgat ccccgaccga gtcgatcgag
     2221 ttcgcg
//
LOCUS       DATBSW010000350        15901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1161133, whole genome shotgun sequence.
ACCESSION   DATBSW010000350 DATBSW010000000
VERSION     DATBSW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161133"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VIF63_09520"
     CDS             <1..1199
                     /locus_tag="VIF63_09520"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY8239662.1"
                     /translation="VGKRITAVLGPTDVVARFAGDTFALLLPGTDADDALAFAERVQL
                     ELRPPFDLDGRTWFISASMGIAVGAPGTAGAGDMLQEAEIALVGAKRDPTRRIAMFDR
                     LTSRHALERLDVEAELWTALERDELTVYYQPILDLRNTRIVGFEALARWQHPLRGLVL
                     PVDFIALAEESDLIVAIGRVILEKACRQAQLWRTRWPEENLMMSVNLSPRQFLDPDLA
                     NGVRQVLGASGLEPCALELEITESSVMDRSETSLGVLQQLRALGVRVVLDDFGTGYSS
                     LAYLRQLPLDTIKIDRSFVTDLDVSDPNVGIVRAVVSLAHGLGITVVAEGIETDEQAR
                     RLRELGCDMGQGYLWAHPADPVRIGTFVSRRLDEGGPKGSPKASAQRRPPNSRSRNRK
                     TLTKSR"
     gene            complement(1139..1873)
                     /locus_tag="VIF63_09525"
     CDS             complement(1139..1873)
                     /locus_tag="VIF63_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY8239663.1"
                     /translation="MTLQPNPRDGQVAEPGGTLRINGVDVRADGQTQVAYLTQAFFWM
                     FAGLLLTAGIATIVRLNPSLLAFAQSNVLPLVIGQLVLVIAIQWGISRISATVALGLF
                     FVYAASLGLVIGLIVSLYTTASVGAAFLSASAMFGGAALYGYTTKRSLAGLGAYLRMA
                     IFGLIAAMVVNIFLGASTIGWIISLVGVVLFTVLTAFHVQRIVKGDLVVATGSVEKAA
                     VVGAVLLYLDFVNVFLFLLRLFGGRR"
     gene            complement(1870..2517)
                     /locus_tag="VIF63_09530"
     CDS             complement(1870..2517)
                     /locus_tag="VIF63_09530"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8239664.1"
                     /translation="MSARSPIDSVVATTKGDDGTTGLLYGGARIGKDDARTEAYGTVD
                     EAIAALGMARANLGVKRRIGTLPAGLGGLADLILRFQRELFVVGAELATNPEQWDRLE
                     DDRTRVSEAMVRSVEATLLQLEANIEMPREFVVPGETPTSAALELARTILRRAERRAV
                     TLSRTGLVPGPNLVPYLNRLADLVWVLARAAEQAESRAATPARRPGAATRKETST"
     gene            complement(2531..2908)
                     /locus_tag="VIF63_09535"
     CDS             complement(2531..2908)
                     /locus_tag="VIF63_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY8239665.1"
                     /translation="MTDTAETEAPAVEPTAATATAPVAEAAAPLFDAERRATELAILD
                     ALRAVVDPEIGMNVVELALIKQILLGDNETEIKMILTTPFCPYAGSMIAQVQEQVESV
                     VEHPVKVTLLAERWDPRDAGLMW"
     gene            complement(3204..4487)
                     /locus_tag="VIF63_09540"
     CDS             complement(3204..4487)
                     /locus_tag="VIF63_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8239666.1"
                     /translation="MRPHNRPPWWPDDEPWPPVDPAELTAEQHRALHGETVSVSGDGS
                     SEARREGPSEWGSAEWRRNQWRSGRWGSGNWGSGNSQQRIRRGFGCIFVLIATLIVSI
                     GVLVLWLLGSLLGIVSDDGLLGTLARSAGLVVLVVGLIALVFGGRFTRRVGVPLAELV
                     EAAGRIEAADYSVRVAEPERGRGELRGLGRAFNAMAARLEAEDATRRRLLADVSHELR
                     TPLAVIQGNLEALLDGVYPPDAAHIDPILDETRVLERLIDDLRTLSLAESGALPLHRE
                     TTEPAILLEDVAAAHRARAAAGKVELRVEVEGGLPSIDIDPVRMRQVVSNLVDNAIRV
                     MPDGGTVTLSAVRRDGSLIIEVTDDGPGIPSDLLPTVFERFSKSTESRGSGLGLSIAR
                     AIVTAHGGTILAISPVPGAANGKPGTTIRISLELG"
     gene            complement(4489..5199)
                     /locus_tag="VIF63_09545"
     CDS             complement(4489..5199)
                     /locus_tag="VIF63_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8239667.1"
                     /translation="MQTVLVVDDEPKIAQLARDYLEHAGFAVVTAGDGPSALHAARVR
                     HPDLVVLDLGLPGLDGLEVMRSIRSTGAMPIVVVTARDTELDKLLGLELGADDYVTKP
                     FSPRELVARVRAVLRRTERATEPRDRIEVGDLVLDVPRLRVTLRGRPIDLTATEFSLL
                     ATMAREPGRVFTRSQLLDAVHGVAFDSYERAIDAHVKNLRRKLEPEPSRPRHVLTVYG
                     VGYRVAEPESAAPDAAQG"
     gene            complement(5305..5766)
                     /locus_tag="VIF63_09550"
     CDS             complement(5305..5766)
                     /locus_tag="VIF63_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239668.1"
                     /translation="MGRAIAGFFLTLVAVGVLIGIGTEIYQAGVAQGIVDAGRFPAAA
                     PVPVAGYGGYHGGFNILGLFFPLLFLFVLFGLIRAAFSRGRGWGHGYGHGYRGGWGSG
                     WDKGPGEGGPSSWREEREQRMADLHRRMHESEGNPGSGGTGSGAPGGSPAT"
     gene            complement(5901..6233)
                     /locus_tag="VIF63_09555"
     CDS             complement(5901..6233)
                     /locus_tag="VIF63_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY8239669.1"
                     /translation="MTIKWVVSDPLVMNGEPFCYGTRLTVRNILELRGAGYTLPRMLK
                     DHPELRRMGVAAAYAYAAKNKARYADFFEADGSIAGPGYTEAEADGLPSQYRIPGVVI
                     KSSDRGPA"
     gene            complement(6235..6717)
                     /locus_tag="VIF63_09560"
     CDS             complement(6235..6717)
                     /locus_tag="VIF63_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239670.1"
                     /translation="MSHPSLGLPPPDMSAGDRAAAAALRSGGTRLRSRAFEAALDLDP
                     TLNDRHGELVRGALLADLDAFVDRLVTAMSTGDPRAMATFADLVAVRYRKRKVGMDDV
                     ITLCEGLRRASAALVELDALPALDASIDAAIAVFRWHRRLAGDARKRHPLLGFIYKGA
                     "
     gene            6781..7332
                     /locus_tag="VIF63_09565"
     CDS             6781..7332
                     /locus_tag="VIF63_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239671.1"
                     /translation="MTTSEPGDRRPARDSPESPSATRRLERPPSERYGRRGTDGAPEG
                     DEPKGASALAGPLVRSVVVAAAGVAALFVLGATLAETLGLLFVSGLTGAAIGLVLARA
                     SAPGTSAPPTPRRTVTWLAVAIAVSAVVIADLAIWANAINEGGTLGFVDYLLTAFGPF
                     VPAEAGLAALTAAWGANAGPVQG"
     gene            7332..7829
                     /locus_tag="VIF63_09570"
     CDS             7332..7829
                     /locus_tag="VIF63_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239672.1"
                     /translation="MEEQPAISFRRPTEADYPTIVRVIDDWWGGRKMDVLLPRLWLQH
                     FTGTSWLAHTDDGKLAGFLIGFHSPDQANVAYCRMIATNPSVRHRGVGRALYERFFED
                     ARQAGRHEVRAITWPGNRASISFHRAIGFEVEVGPGSQNLYGTPAQAGYDFDREDRTI
                     LVRTV"
     gene            7841..8284
                     /locus_tag="VIF63_09575"
     CDS             7841..8284
                     /locus_tag="VIF63_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEY8239673.1"
                     /translation="MRGILIRVAIVGAIIVAFIVLRPLLPGNASTLAVGDCFDVPTGT
                     ETIDDVQHHPCTEAHGGEVVHSGNYEPATSTYPTDAEFEDYVADKCLPAFEAYTGRTY
                     ESATDLNIEWFVPTSESWADGNKKVVCYALNADGSTMTKSVKVAS"
     gene            complement(8286..8615)
                     /locus_tag="VIF63_09580"
     CDS             complement(8286..8615)
                     /locus_tag="VIF63_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239674.1"
                     /translation="MTRDELVRDTRQLIAEGERLVANPSQGALKVWLQLTDDLLVRAW
                     GSMDRYHLAWLQVGRPSAIVRGREMTADEESGYVREVASAKTAVLRMSVEALTRHGMP
                     FVGETRD"
     gene            complement(8612..9538)
                     /locus_tag="VIF63_09585"
     CDS             complement(8612..9538)
                     /locus_tag="VIF63_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY8239675.1"
                     /translation="MSDGAHEELEGRHVWIDGALTPAGARHLSAFDRGFQLGDGVFET
                     LRARAGRPTELDEHLARLRRSAAGLDFELPATLEADIQVGIAALLAADGLAGPDGDAS
                     IRITASRGTVSGRGLLPPGPSTATIVIQAWPVPPPPAGHLEQGLHLVVSAVRRDPASP
                     LAALKTTSRADYVYARLEARRASADDALFLTTDGRISEATSANLFLVRQAAGGGATEL
                     ATPGLDCAILPGTTRTWILQWARGAGLEPVEGSLVPGDLAAADEAFLCSSVAGILPVT
                     RFDGAPIGTGRPGPWTGRARGAREAFIRDPAR"
     gene            complement(9535..11013)
                     /locus_tag="VIF63_09590"
     CDS             complement(9535..11013)
                     /locus_tag="VIF63_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY8239676.1"
                     /translation="MQRGEIDRGAEGGGLAAGARLGPGVRLAEIPGLAGLAPMDVAER
                     FRDLPGLVLFESARPGRNARWSYLSADPVAVLDAPAEGADAFVSARRLLGRLCADPVA
                     GPDRPPFLGGLAGFLGYDLGRRFERLPSIAIADQELPQLRLALHDWVIAWDRHSGRAW
                     LGGRAVDERIARLDRRLADVRERALGQRSDATMMRSDAPGASGSVDAEIELRSGLDRA
                     AYEAGVGQIRQWIADGEIYQANLTRRLIAPFRGDPWPLYRRLRTGDPALFAAFVDLGP
                     SPITGAPRAIVSASPEPFMSVAPGGEVATNPIKGTRPRGRDRDDDRRLARELLASEKD
                     RAENVMIVDVLRNDLGRVCRPGSVRVPRLCRLERTAAVQHLVSTVTGRLEAGRDAFDL
                     LAASFPGGSITGAPKIRAMELLERLEPVRRGPYTGIAGWIGPDGAMGTSILIRSFVAD
                     GEHLSLHVGGGITWRSDPAAEWDETVAKALGPLRAIGAREVA"
     gene            11086..12447
                     /locus_tag="VIF63_09595"
     CDS             11086..12447
                     /locus_tag="VIF63_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239677.1"
                     /translation="MTSLRTPATRLAAIALAILLTACGQSVASPSVAPATAAPTSTMP
                     LVGPTTGAPTTAPSPTRDEGWRADIAALVPGMAAIHPDLTHSVSRAELDAAATALSAT
                     VAAATDDELMVGVLRIVAMVSSAGCDAHTGAFVWGSGTYPVDSMPLRLWLFEDAVVIV
                     DALPPYANLIGARIDTIEGHPTADVMAQVDPIVPRDNSQTVRLLMPRYLLIPQILRGL
                     GLADDGPISLGVTPVDGPAATIDIEPIPMAAYNEWAGPYGLHLPVDPDVRYLSRIDDA
                     LWWELLEDDETLYVQYNRVDRLPGTLVTDLQHVLDDASVARVLLDIRHNYGGELSALN
                     AMTPILVGAAEGDPNRLFVFIGRNTFSGGSLLAARLEHDAQATLIGEATGGCPTIWSD
                     PTDVPLPWSGITVSVAGDTAVGVDPNDPRLTVEPSVNAVLNVDEWREGIDPALELFDV
                     EAP"
     gene            12444..12926
                     /locus_tag="VIF63_09600"
     CDS             12444..12926
                     /locus_tag="VIF63_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239678.1"
                     /translation="MSDALGTSILFDVFALNQAVGRLLAAAMRDGPLTPGEYAIYSAV
                     FELEAASPTELAARLGMRLTTFMDQLRLLESRGHAHRVENPRDRRSYRVVLTNAGLEA
                     HRAANRRFEEAFQAFVAHLPDGEAAAKKGVLVVRRAAEAALTGYSEGTSPRPKVGRAG
                     "
     gene            complement(12892..13326)
                     /locus_tag="VIF63_09605"
     CDS             complement(12892..13326)
                     /locus_tag="VIF63_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239679.1"
                     /translation="MPDDTRAQVPGMPGPAPSPPVIAESGDPFGALRVVEVVSRIPRG
                     RPIGLSTIVDRLNATHLDWLFDERVVADALVQLQANWMADYRNASGIVLDDGGATSGA
                     SVTIEDSSRVDPWIARQAQRLHAACREVLDDFSRRDRLSGEG"
     gene            complement(13339..15021)
                     /gene="ablA"
                     /locus_tag="VIF63_09610"
     CDS             complement(13339..15021)
                     /gene="ablA"
                     /locus_tag="VIF63_09610"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY8239680.1"
                     /translation="MDAQEIKAAVAHQNAAAAAAEAPFVNRRDPALALGADGLPARSR
                     RAPIWEDVPDEKWDDWRWQLSNRINSLDEVAAVLNLTDEEKDGLTADDKFRVDITPYF
                     ASLIDPDDPNDPIRRQVIPLGREHQAFTAMMEDSLAEDRHSPVPGLVHRYPDRVLMLV
                     TTQCASYCRYCTRSRIVGDPTQNFNSRDHEAQLEYLRRTPQVRDVLISGGDGLTLAPK
                     LFEKILRGLREIPHIEIIRIGSRVPVFLPQRIDDELCAMLEKYHPLWINLHFNHPNEI
                     TPEVSRAVDKLTKAGLPVGNQSVLLAGVNDCVHIQRALVHRLVENRIRPYYLYQCDLV
                     EGSGHFRTPVGKGLEIMEGLRGHTSGYAVPTFVIDAPGGGGKIPVMPNYLISYSDHKV
                     VLRNYEGYITTYEEPPTYERHDEAACSYCQHQRSEPGQSGVLGLLQGERMWIEPKGFS
                     ELHTRGNTEAHRLQDPSKWVPFGVGAIEGTSGRPLPVLEAGDLPDAAEMTERAATETN
                     AGIVEPTDILTVEQLAANGERAASGNGHVYGPGEEPRPREGEPTASAHGELL"
     gene            complement(15154..>15901)
                     /locus_tag="VIF63_09615"
     CDS             complement(15154..>15901)
                     /locus_tag="VIF63_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239681.1"
                     /translation="SPMFQLPLLVPHRTMLDGLPVNIRSSQMATIEAHGSLTPNPIGP
                     LFTDNVGEVQLDYTPNAEAAQGQGNEQTAVVLVVAEIEFREILQYMYAAGPAANLGFG
                     SRLIPELLTIQWHEAAPSAGTFHVTLTGKKAGAGTYSGTAEIICVETPSSSGGLLYTA
                     VTLPDRATGVSHISLGQNDGGGADVGVEVTAQTEFGPWGGSSRMAGQTATVTAVRNGP
                     LVEMHGTASVTDTAGGVYTTEVSLTCPLLT"
BASE COUNT         2369 a         5544 c         5674 g         2314 t
ORIGIN      
        1 aggtcggcaa gcgcatcacg gcggtccttg ggccgacgga cgtggtggcg agattcgccg
       61 gtgacacgtt cgccctgctc ctccccggca ccgacgccga cgacgcactc gccttcgcgg
      121 agcgggtcca gctcgagctc aggccgccgt tcgacctcga cggccggaca tggtttatca
      181 gcgccagcat gggcatcgcc gtcggcgcgc ccggcaccgc cggggccggc gacatgctcc
      241 aggaggccga gatcgcgctc gtcggtgcga agcgcgaccc gacacggcgc atcgcgatgt
      301 tcgaccgcct gaccagccgg catgcgctcg agcgcctcga cgtcgaggcc gagctgtgga
      361 ccgcgctgga gcgcgacgag ctgacggtct actaccagcc catcctcgac ctgcggaaca
      421 cgcgcatcgt gggcttcgag gcgctcgcgc gatggcagca cccgctgcgc gggctcgtgc
      481 tgccggtcga cttcatcgcc ctcgccgagg aatccgacct gatcgtggcg atcggccggg
      541 tcatcctcga gaaagcctgc cgccaggccc agctctggcg cacgcgctgg cccgaggaga
      601 acctgatgat gagcgtgaac ctctcgcccc gccagttcct cgacccggac ctcgcgaacg
      661 gcgtccgcca ggtcctcggc gcgtcgggcc tcgagccgtg cgcgctggag ctggagatca
      721 ccgagagcag cgtgatggac cggtcggaga cgagcctcgg cgtcctccag cagctgcggg
      781 cgctcggcgt ccgcgtcgtc ctcgacgact tcgggaccgg ctattcctcg ctcgcgtacc
      841 tgcgccagct gccgctggac acgatcaaga tcgaccgttc gttcgtcacc gacctcgacg
      901 tctcggaccc gaatgtcggg atcgtccggg ccgtcgtgtc actcgcccac ggcctcggca
      961 tcaccgtcgt cgccgagggc atcgagaccg acgagcaggc gcgtcgcctg cgcgagctcg
     1021 gctgcgacat gggccagggc tacctgtggg cgcacccggc cgatcccgtg cggatcggga
     1081 cgttcgtcag ccgccgcctc gacgagggag gcccgaaagg aagcccgaag gcctcggctc
     1141 agcggcgacc gccgaacagc cggagcagga acaggaagac gttgacgaag tccaggtaga
     1201 gcaggaccgc gccgacaacg gcggccttct cgaccgagcc cgtggcaacc acgaggtcgc
     1261 ccttcacgat ccgctggacg tggaacgcgg tgaggaccgt gaacaggaca accccgacga
     1321 gcgagatgat ccagccgatc gtgctcgcgc cgaggaagat gttgaccacc atcgccgcga
     1381 tgagcccgaa gatcgccatc cgcaggtagg cgccgagccc ggcaagggat cgcttggtgg
     1441 tgtagccgta cagggccgcg ccgccgaaca tcgcggacgc ggacaggaac gccgcgccga
     1501 ccgaggcggt cgtatacagc gagacgatga gcccgatgac cagcccgagg ctcgccgcgt
     1561 acacgaagaa caggccgagg gcgaccgtcg cgctgatccg gctgatgccc cactggatcg
     1621 cgatcacgag gacgagctgg ccgatgacga gcggcagcac gttgctctgg gcgaacgcga
     1681 gcaggctcgg gttcaggcgc acgatcgtgg cgatgccggc cgtgagcagg agcccggcga
     1741 acatccagaa gaacgcctgg gtgaggtacg ccacctgggt ctggccgtcg gcacggacgt
     1801 cgacgccgtt gattcggagg gtgccgcccg gctcggcgac ctgcccgtct cgaggattgg
     1861 gttggagggt cacgtcgagg tctcctttcg agtcgcggcg cccggtcgtc gggccggcgt
     1921 cgctgcgcga gattctgctt gttccgccgc gcgcgcgagg acccagacga ggtccgcgag
     1981 gcgattgagg tagggcacga gattggggcc ggggacgagg ccggttcgtg agagcgtcac
     2041 ggcgcgtcgc tctgcgcggc gcaggatcgt tcgggcgagc tcgagggcgg cgctcgtcgg
     2101 ggtctcgccg ggcacgacga actccctggg catctcgatg ttggcttcga gctggaggag
     2161 cgtcgcctcc acgctgcgga ccatggcttc ggagacgcgg gtgcggtcgt cctccagccg
     2221 atcccactgc tccgggttcg tggccagctc ggcaccaacg acgaacagct cgcgctggaa
     2281 ccgcaggatc aggtcggcga gcccaccgag gccggcgggc agcgtgccga ttcggcgctt
     2341 gacgccgagg ttcgcgcggg ccatgccgag cgcggcgatg gcctcgtcga ccgtgccgta
     2401 tgcctcggtg cgggcgtcgt ccttgccgat cctcgcgccg ccgtacagga gtccggtggt
     2461 cccgtcatcg cccttcgtcg tggcgacgac ggagtcgatc gggctgcgtg cgctcaccgg
     2521 ctgatcgcgc ctaccacatc agacccgcgt cacgcgggtc ccatcgctcg gcgagcaacg
     2581 tcaccttcac cggatgctcg acgaccgact ccacctgctc ctgcacctgc gcgatcatcg
     2641 aacccgcgta cggacagaac ggcgtggtca ggatcatctt gatctcggtc tcgttgtccc
     2701 cgagcaggat ctgcttgatc agcgccagct cgaccacgtt catcccgatc tcgggatcga
     2761 ccacggcgcg tagcgcatcg aggatcgcga gctcggtggc gcgacgctcg gcgtcgaaca
     2821 gcggcgcggc ggcctcggcg acgggtgcgg tagctgttgc ggccgtgggc tcgacggccg
     2881 gagcctcggt ttcagcggtg tcggtcatcg tgtgcagagt acaacaaggt gcccgaatac
     2941 ccgaccggcg ggcttgggat tagcgtgact gtcggccgtg ggcgtgccga gggagcccaa
     3001 cgttcgtatg cgtggattcg ccgcaagaat cgcgtgcgcc acgggcaatg cccgttgccg
     3061 tcatacgagc ggtcccttcg tatgctggcg acgcgcatgg cccgcagcaa catgaggcgg
     3121 gacgccgatt tcacgcatac gaacgttgga ggaagggcag gccgcgcagg cagcggcagg
     3181 ccgggcaggg cggccctgac gcgtcagccg agctcgagcg agatccggat cgtcgttccg
     3241 ggtttgccgt tggccgcgcc cggcacgggc gagatcgcga ggatcgtgcc gccgtgggct
     3301 gtgacgatcg cgcgggcgat cgagaggccg aggccggagc cgcgcgactc ggtcgacttc
     3361 gagaaccgct cgaacacggt cggcagcaga tcggacggga tgcctgggcc gtcgtcggtc
     3421 acctcgatga tgagcgagcc gtcgcggcgg accgcggaga gcgtcacggt gccgccgtcg
     3481 ggcatgaccc ggatcgcgtt gtcgaccagg ttcgagacga cctggcgcat gcgcacggga
     3541 tcgatgtcga tcgagggcag gccgccttcg acctcgaccc gcagctcgac cttgccggcc
     3601 gctgcgcgcg cccgatgggc ggccgcgacg tcctcgagga ggatcgccgg ctcggtcgtc
     3661 tcgcgatgga gcggcagcgc gccggactcg gcgaggctca gcgtccggag atcgtcgatg
     3721 agccgctcga ggacgcgcgt ctcgtcgaga atcgggtcga tgtgggcggc gtcgggcggg
     3781 tacacgccgt cgagcagcgc ctcgaggttg ccctggatca cggcgagcgg cgtgcgcagc
     3841 tcgtggctca cgtccgcgag caggcgtcgg cgcgtcgcat cttcggcctc gagacgcgcc
     3901 gccatcgcat tgaacgcgcg accgaggccg cggagctcgc cacgcccgcg ctccggttcg
     3961 gccacgcgga ctgaatagtc ggcggcctcg atgcggcctg ccgcctcgac cagctcggcc
     4021 agcggcacgc cgacccgccg cgtgaaccgg ccgccaaaga ccagggcgat cagcccgaca
     4081 acgagcacga cgaggcccgc cgatcgagcg agcgtcccga gcaaaccgtc gtccgacacg
     4141 atgccgagca ggctgccgag cagccacagg accagtaccc cgatcgagac gatgagcgtc
     4201 gcgatcagca cgaagatgca gccgaagcct cgacgaatgc gctgctgtga attgcccgac
     4261 ccccagttgc ccgagcccca gcggccgctc cgccactggt tgcggcgcca ttcggcgctc
     4321 ccccactcgc tcgggccttc gcgccgggcc tcgctcgagc cgtcgcccga gacggacacg
     4381 gtttcgccgt gcagcgctcg gtgctgctcc gcggtcagct cggcagggtc gacgggaggc
     4441 cagggctcgt cgtcgggcca ccagggcgga cggttgtgcg gtcgcacgtc agccctgcgc
     4501 cgcgtccggg gcggctgatt ccggttccgc gacccgatag ccgacgccgt acaccgtgag
     4561 cacgtggcgc ggccggctcg gctccggttc cagcttccgg cggaggttct tcacgtgggc
     4621 atcgatcgcc cgctcgtacg agtcgaatgc cacgccgtgg accgcgtcga gcagctgcga
     4681 ccgggtgaag acgcgccccg gttcgcgggc catggtcgcc aggagggaga actcggtcgc
     4741 ggtgaggtcg atcgggcggc cgcggagcgt aacgcgcagt cgcggcacgt cgaggacgag
     4801 gtcgccgacc tcgatgcggt cacgcggctc ggtcgcgcgc tccgtgcgcc ggagcacggc
     4861 tcgaacccgg gcgaccagct cgcgcgggct gaacggcttg gtcacgtagt cgtccgcccc
     4921 gagctcgagc ccgagcagct tgtcgagctc ggtgtcgcgg gcggtgacga cgacgatcgg
     4981 catcgcgccg gtcgaccgga tcgagcgcat cacctcgaga ccgtcgaggc cgggcagccc
     5041 gaggtcgagc acgacgagat ccgggtgtcg gacgcgggcg gcgtgcagcg ccgacggccc
     5101 gtcgcccgcg gtcacgacgg caaagccggc gtgctcgagg taatccctgg cgagctgggc
     5161 gatcttcggt tcgtcatcga cgacgagcac ggtctgcatc ggcggtcact ctagtcgcgc
     5221 gggatgcgct ccggcgcgga agacggaagc gccggccggc gcccgaaggc gcggccggcg
     5281 cgggaagtgg gttgaagcgg ccggtcaggt ggccggcgag ccgcccggcg ctccggagcc
     5341 ggtcccgccc gagccggggt tcccctcgga ctcgtgcatg cggcggtgca gatcggccat
     5401 gcgctgctcg cgctcctcgc gccagctcga cgggccgccc tcgcccgggc ccttgtccca
     5461 gcccgagccc cagccaccgc ggtagccgtg cccgtagccg tggccccagc ctcggccgcg
     5521 cgagaacgca gcccggatga gcccgaacag cacgaacagg aacagcagcg ggaagaacag
     5581 gcccaggatg ttgaagccgc cgtggtagcc gccgtagccg gcgaccggca ctggtgccgc
     5641 ggccgggaag cgcccggcat cgacgatgcc ctgtgcgacg cccgcctgat agatctccgt
     5701 cccgatgccg atcagcacgc cgacggccac cagggtgagg aagaagcccg caatggctcg
     5761 tcccatgtca tctccttgca tgtgaaaagg tcgatgagca caggctggcg cggggtcgtg
     5821 aagcgaatgt gaacgagacg tgcaagacct gcgatgaagg tcagggaacc ggtggaggct
     5881 ctccgaggac cgaggtgaag ctacgcgggc cctcggtccg aggacttgat cacgactccg
     5941 gggatgcggt actggctcgg caggccgtcg gcctccgcct cggtgtaccc aggaccggcg
     6001 atggacccgt cagcctcgaa gaagtccgcg taccgagcct tgttcttcgc ggcatacgcg
     6061 tatgccgccg ccacgcccat ccggcgcagc tctgggtggt ccttgagcat ccgcgggagc
     6121 gtgtagcccg cgccgcgcag ctcgaggatg ttgcgaacgg tgagccgcgt cccgtagcag
     6181 aacggctcgc cgttcatgac gagcgggtcc gacacgaccc acttgatggt cacgtcacgc
     6241 gcccttgtag atgaacccga gcagcgggtg gcgcttgcgc gcgtcgcccg cgagccgccg
     6301 atgccagcgg aagaccgcga tcgcggcgtc gatggacgca tcgagcgcgg gcagggcgtc
     6361 gagctcgacg agcgcggcgg aggcgcgccg cagcccctca cacagcgtga tcacgtcgtc
     6421 catcccgacc ttgcgcttgc ggtaccgaac cgcgacgagg tccgcgaacg tggccatcgc
     6481 ccgcgggtcg ccggtcgaca tggcggtcac gagccgatcg acgaaggcat cgaggtcggc
     6541 gagcagcgcc ccacggacga gctcaccgtg ccggtcgttc aaggtggggt cgagatcgag
     6601 ggccgcctcg aaggcgcggc tccggaggcg cgtgccgcca ctcctgagcg cggccgcggc
     6661 cgcgcgatcg ccggccgaca tgtcgggcgg gggcaggccg agactcgggt ggctcacgcg
     6721 ccggagtcta tcgcgcgcct tcgggcgggc gctccgagcc ggcgccggta cgatgacctc
     6781 gtgaccacct cggagccggg cgaccgaagg cctgcgcgcg attccccgga atcgccctcg
     6841 gcgacacgac ggctggaacg cccgccatcg gagcgctacg ggcggcgcgg cacggacggc
     6901 gcaccggagg gcgacgagcc gaaaggtgca tcagcgctcg cgggcccgct cgtccgttcc
     6961 gtcgtcgtgg ccgcggctgg cgtggcggcg ttgttcgtgc tcggcgcgac gctcgccgag
     7021 accctgggcc tgctgttcgt gtccgggctc accggcgcgg ccatcgggct cgtgctggcg
     7081 cgcgcgtccg cgcccgggac ctcggcgccg ccaacgccac gccggaccgt gacctggctg
     7141 gccgtcgcga tcgcggtcag tgcggtcgtc atcgccgatc tcgcgatctg ggcgaacgcc
     7201 atcaacgagg gcggcaccct cggcttcgtc gactacctgc tgacggcgtt cgggccgttc
     7261 gtgcccgccg aggcgggcct cgcggcactg acggcggcat ggggcgcgaa cgccggaccg
     7321 gtccagggct gatggaggag cagcccgcga tcagctttcg acgccccacc gaggcggact
     7381 acccgacgat cgtgcgcgtc atcgacgact ggtggggcgg ccggaagatg gatgtcctgc
     7441 tgccccgact ctggctccag cacttcaccg ggacgtcctg gctggcgcac acggacgacg
     7501 gcaagcttgc cggcttcctg atcggcttcc actcgcccga ccaggcgaac gtcgcgtact
     7561 gccggatgat cgcgaccaac ccgagcgtgc ggcatcgagg cgtcggccga gcgctgtacg
     7621 agcgattctt cgaggacgcc cggcaggccg gccggcacga ggtccgcgcg atcacgtggc
     7681 cgggcaatcg agcctcgatc tcgttccatc gtgccatcgg gttcgaggtc gaggtgggac
     7741 ccggcagtca gaatctctat ggaacacccg cacaggcagg gtacgatttc gaccgggagg
     7801 atcgaaccat cctggttcgg acggtctagg gagggcgggc atgcgcggga tcctgattcg
     7861 ggttgcgatc gtcggcgcca tcatcgtcgc gttcatcgtc ctgcggccat tgctgcccgg
     7921 caatgcgagc acgctggccg tgggcgactg cttcgacgtt ccgaccggca ccgagacgat
     7981 cgacgacgtg cagcaccatc cgtgcaccga ggcacacggc ggcgaggtcg tccactccgg
     8041 caactacgag ccggcaacct cgacatatcc gaccgacgcc gagttcgagg actacgtcgc
     8101 ggacaagtgc ctcccggcgt tcgaggccta taccggccgc acgtacgagt cggcaaccga
     8161 cctgaacatc gaatggttcg tgccgacatc ggagagctgg gccgacggga acaagaaggt
     8221 cgtttgctac gccctcaacg cggacggctc gaccatgacg aagtcggtca aggtcgcctc
     8281 gtaggtcaat cccgggtctc gcccacgaac ggcatgccat ggcgggtgag cgcttcaacg
     8341 ctcatccgca gcacggccgt cttggccgag gccacctcgc gcacatagcc tgactcctcg
     8401 tcggcggtca tctcgcggcc gcgcacgatc gcgctcgggc gcccgacctg gagccaggcc
     8461 aggtggtagc ggtccatcga gccccaggcg cggacgagca ggtcgtcggt gagctggagc
     8521 cagaccttca gcgcgccctg gctcgggttg gccacgagcc gctcgccctc cgcgatcagc
     8581 tggcgcgtgt cgcggacgag ctcgtcccgc gtcatcgcgc ggggtcgcgg atgaatgcct
     8641 cgcgcgcccc gcgtgcgcgt cccgtccacg ggccgggacg tcccgtgccg atcggtgcgc
     8701 cgtcgaaccg cgtcaccggc aggatgcccg cgacgctgga gcacaggaac gcctcgtcgg
     8761 cggccgcgag gtcccccgga acaagcgagc cctcgaccgg ctcgaggccc gcgccgcgcg
     8821 cccactggag gatccaggtg cgcgtcgtgc ccggcaggat cgcgcagtcg aggccgggcg
     8881 tggcgagctc cgtggccccg ccgccggccg cctgccgcac gaggaagagg ttcgcgctcg
     8941 tcgcctcgga gatgcgcccg tcggtcgtca ggaagagggc gtcgtcggcg cttgcgcgac
     9001 gggcctcgag gcgcgcgtag acgtagtcgg cgcggctcgt ggtcttcagc gcggcaagcg
     9061 ggctggccgg gtcgcgccgc accgccgaca ccacgaggtg caggccttgc tcgaggtggc
     9121 cggcgggcgg aggcgggacg ggccacgcct ggatgacgat cgtggcggtc gacggacctg
     9181 gtggcaggag gccgcggccg cttaccgttc cgcgactggc cgtgatccgg atcgaggcat
     9241 caccgtcagg cccggcgagc ccgtcggcgg cgagcagcgc ggcgatgccg acctgaatgt
     9301 cggcttcgag cgtcgctggc agctcgaaat cgaggccggc agccgagcgg cgcaggcgcg
     9361 ccagatgctc atcgagctcg gtcgggcgcc cggcgcgggc gcggagcgtc tcgaaaacgc
     9421 cgtcaccgag ctggaacccg cgatcgaacg cggagaggtg gcgggcgccg gccggcgtga
     9481 gcgcgccgtc gatccacacg tgacggccct cgagctcttc gtgcgccccg tcgctcacgc
     9541 gacctcgcgg gcgccgattg cgcgcagtgg cccgagcgcc ttggcgacgg tctcgtccca
     9601 ctcggcggca ggatcgctgc gccaggtgat gccgccgccg acgtgcaggc tcaggtgctc
     9661 gccatcggcg acgaagctgc ggatcaggat gctcgtgccc atcgccccgt cgggaccgat
     9721 ccagccggcg atgccggtgt acggcccgcg ccgaaccggc tcgagccgct cgaggagctc
     9781 catcgcccgg atcttgggcg cgcccgtgat ggaaccgccg gggaacgaag cggccaggag
     9841 gtcgaacgcg tcgcggccgg cctcgagccg gccggtgacg gtcgagacga ggtgttgcac
     9901 cgccgccgtc cgctccaggc ggcacaggcg cgggaccctg accgaccccg gacggcagac
     9961 tcgcccgaga tcgttgcgca ggacgtccac gatcatcacg ttctcggcgc gatccttctc
    10021 ggaggcgagc agctcccgcg cgagccgccg atcgtcgtct cgatcgcgac cacgcgggcg
    10081 tgtgcccttg atcgggttcg tcgcgacctc gcccccgggc gcgacggaca tgaacggctc
    10141 cggcgaggcg gagacgatcg cgcgcggggc gccggtgatc gggctcggtc cgaggtcgac
    10201 gaaggcggcg aacagcgccg gatcgcccgt ccgcagccgg cggtacagcg gccacggatc
    10261 gccgcggaat ggcgcgatga gccggcgcgt cagattggcc tggtagatct cgccgtcggc
    10321 gatccactgc cggatctgcc cgacgcctgc ctcgtatgcg gcccgatcga gcccggaacg
    10381 cagctcgatc tctgcatcga cggacccgct cgcacccggc gcatcgctcc gcatcatcgt
    10441 cgcgtcggac cgctggccga gggcgcgctc acggacgtcc gccagccgac ggtcgagccg
    10501 cgcaattcgt tcgtcgaccg ctcttccacc gagccaggca cggcccgaat ggcgatccca
    10561 cgcgatgacc cagtcgtgga gcgcgagccg cagctgcggc agctcctggt cggcgatcgc
    10621 aatcgacggc aggcgctcga accgacgacc gaggtcgtag ccgaggaacc ccgcgagccc
    10681 gccgaggaac ggcgggcgat ccgggccggc gaccggatcg gcgcacagcc ggccgagcaa
    10741 cctgcgagcg ctcacgaacg cgtcggcgcc ctcggccggc gcgtcgagga ccgcgaccgg
    10801 gtcggcgctg aggtacgacc agcgggcgtt gcggcccggg cgggcgctct cgaagaggac
    10861 gaggcccggc aggtcccgga accgctcggc gacgtccatc ggggcaagcc cggccagccc
    10921 cgggatctcg gcgaggcgca cgccgggccc gagtcgcgcg cccgccgcaa gccccccgcc
    10981 ctcggcaccc ctgtcgatct cgccgcgctg catgtgccgg atcatatcga ccgccgctcc
    11041 cggcccggct tgccagcaga tactacggac ccgtagtatc tgccgatgac atcccttcgt
    11101 acgcccgcga cccgcctcgc ggcgatcgcc ctcgcgatcc tcctgaccgc gtgcggccag
    11161 agtgttgcca gtccatccgt cgccccagcg accgctgctc cgacgagcac gatgcccctg
    11221 gtcggcccga cgaccggagc gccaacgacg gcgccgtcac cgacccgcga cgaaggctgg
    11281 cgcgcggata tcgccgcgct cgtgccgggc atggcggcga tccaccccga cctgacccac
    11341 agcgtgagcc gtgccgagct cgacgccgcg gccaccgccc tctcggcaac cgtggccgcc
    11401 gcgaccgacg acgagctgat ggtcggcgtg ctccggatcg tggccatggt cagctcggcc
    11461 ggctgcgatg cgcataccgg cgcgttcgtc tgggggagcg gcacgtatcc ggtcgacagt
    11521 atgccgttgc ggctctggct gttcgaggac gcggtcgtga tcgtcgatgc cctgccgcca
    11581 tacgcgaacc tgatcggcgc gcggatcgac acgatcgagg gccacccgac cgccgacgtc
    11641 atggcccagg ttgatccgat cgtgccccgc gacaacagcc agaccgtgcg cctgttgatg
    11701 ccccgatacc tgctcattcc gcagatcctc cgcgggctcg gcctcgccga tgacggcccg
    11761 atctccctgg gcgtgactcc ggtcgatgga ccggccgcca ccatcgacat cgagcccatc
    11821 ccgatggccg cctacaacga gtgggccggc ccgtacggcc tgcacctgcc ggtcgacccg
    11881 gacgtgcgct acctgtcgcg gatcgacgac gcgctgtggt gggagctgct cgaagacgat
    11941 gagacgctgt acgtccagta caaccgcgtt gatcggctgc cgggcaccct cgtgaccgat
    12001 ctccagcacg tgctcgacga cgcctcggtc gcgagggtcc tgctcgacat tcgccacaac
    12061 tacggcggcg agttgtccgc gctcaacgcg atgacgccga tccttgtcgg tgcggcggag
    12121 ggcgacccga accgcctgtt cgtgttcatc gggcgcaaca cgttctcggg cggcagcctg
    12181 ctggcggccc gcctcgaaca cgacgcccag gcgacgctca tcggcgaggc gaccggcggc
    12241 tgccccacga tctggagcga tccgacggac gtccctctgc cgtggtcggg cattaccgtc
    12301 agcgttgccg gcgacacggc ggtgggcgtg gatccgaacg acccgcgcct caccgtcgag
    12361 ccgagcgtca acgctgtcct caacgtcgat gaatggcggg aagggatcga tccagccctc
    12421 gagctgttcg acgtcgaggc gccgtgagcg acgccctggg cacgtcgatc ctgttcgacg
    12481 tgttcgcgct caaccaggcc gtcggccggc tgctcgccgc cgcgatgcgc gacggaccgc
    12541 tcacgcccgg cgagtacgcg atctacagcg cggtgttcga gctcgaagcg gcgtcaccga
    12601 ccgagctcgc ggcgcggctc ggcatgcgcc tcacgacgtt catggaccag ctgcgactgc
    12661 tggagagccg gggccacgcc catcgagtcg agaacccgcg cgatcgccgt tcataccgag
    12721 tcgtgctcac gaacgcgggt ctcgaggcgc accgggccgc gaaccgccga ttcgaggaag
    12781 cattccaggc gttcgtggcc cacctgcccg atggtgaagc ggccgcaaag aaaggtgtgc
    12841 tcgtcgttcg ccgggccgcc gaagccgcgc tgaccgggta ctcggagggg actagccctc
    12901 gcccgaaagt cggtcgcgcc ggctgaagtc gtcgaggacc tcgcggcagg cagcatgcag
    12961 gcgctgcgcc tggcgcgcga tccacggatc gacgcgggag gagtcttcga tcgtgaccga
    13021 ggcgccggac gtggcgccac cgtcatcgag cacgatcccg ctcgcattgc ggtagtccgc
    13081 catccagttc gcctggagct ggacgagcgc gtccgcgacg acccgctcgt cgaacagcca
    13141 gtcgagatgg gtcgcgttga gccgatcgac gatcgttgac aggccgatcg gtcgcccacg
    13201 agggatccgc gagacgacct cgacgacccg tagcgccccg aaagggtcgc ccgattcggc
    13261 gatcaccggc ggtgagggcg ccggtccggg catgcccggg acctgagcgc gagtgtcgtc
    13321 tggcacccgt tcagcctgct agagcagctc gccgtgcgcc gatgcggtcg gctcgccttc
    13381 gcgcggccgg ggctcctcgc cgggaccgta cacgtgcccg ttgcccgacg cagctcgttc
    13441 gccgttcgcg gcgagctgct ccacggtcag gatgtcggtc ggctcgacga tgcccgcatt
    13501 cgtctccgtt gccgcccgct cggtcatctc ggcggcgtcg ggcaggtcgc ccgcctcgag
    13561 gaccggcagc ggccgccctg aggttccttc gatcgcgccg accccgaacg gcacccactt
    13621 cgatgggtcc tggaggcggt gggcctcggt gttgccgcgt gtgtggagct cgctgaagcc
    13681 cttcggctcg atccacatcc gctcgccctg gagcaggccg agcacgccgg actggcccgg
    13741 ctccgagcgc tgatgctggc aatacgagca cgccgcctcg tcatggcgct cgtacgtcgg
    13801 cggctcctcg taggtcgtga tgtagccctc gtagttgcgc agcacgacct tgtggtccga
    13861 gtagctgatc aggtagttgg gcatgaccgg gatcttgccg ccgccaccgg gcgcatcgat
    13921 gacgaaggtc gggaccgcgt aacccgaggt gtggccgcgc aggccctcca tgatctcgag
    13981 gcccttgccg acaggcgtcc ggaagtgccc ggagccctcg acgaggtcgc actggtacag
    14041 gtagtacggc cggatccggt tctcgacgag ccgatggacg agcgctcgct ggatgtgcac
    14101 gcagtcgttc acgccggcga gcaggacgct ctggttgccg acgggcaggc ccgccttcgt
    14161 gagcttgtcg accgcccggc tcacctctgg cgtgatctcg ttcgggtggt tgaagtggag
    14221 gttgatccac agcgggtggt acttctcgag catcgcgcag agctcgtcgt cgatgcgctg
    14281 gggcaaaaag accggcaccc gcgagccgat gcggatgatc tcgatgtgcg ggatctcgcg
    14341 gaggccgcgc aggatcttct cgaacagctt cggcgcgagg gtcaggccat ccccgcccga
    14401 gatcaggacg tcgcgcacct ggggcgtgcg gcgcaggtac tcgagctggg cctcgtggtc
    14461 gcgggagttg aagttctggg tcgggtcgcc gacgatccgc gaccgcgtgc agtagcggca
    14521 gtaactcgcg cattgggtgg tgacgagcat caggacgcgg tcggggtagc ggtggacgag
    14581 gccggggacc ggcgagtggc ggtcctcggc gagcgagtcc tccatcatcg cggtgaaggc
    14641 ctggtgctca cgcccgagcg ggatgacctg gcggcggatc gggtcgttcg ggtcgtcggg
    14701 gtcgatcagg ctcgcgaagt acggcgtgat gtcgacccgg aacttgtcgt cggcggtcag
    14761 gccgtccttc tcctcgtcgg tgaggttcag gaccgcggcg acctcgtcga gcgagttgat
    14821 ccggttgctg agctgccagc gccagtcgtc ccatttctcg tcggggacgt cctcccagat
    14881 tggcgcgcgg cggctgcgcg ccggcaggcc gtcggcgccg agggcgagcg cgggatcgcg
    14941 tcggttgacg aacggcgcct ccgccgcggc cgctgccgcg ttctggtggg cgactgcggc
    15001 cttgatttcc tgggcgtcca cgcgaggcga tctccgatgc gagcggctca gtgcataaag
    15061 tctatgcact ctgcaagtcg gcggatcata gcgcgagggt cccggccggg tccagccgat
    15121 cggcgagcga cagcgtgctt ggaacgtcgc cgttcaggtg agcagggggc aggtcaggga
    15181 gacctcggtc gtgtagacgc cgcccgcggt atccgtgacg ctggccgtcc cgtgcatctc
    15241 gacgagcgga ccatttcgta ccgccgtcac ggtcgcggtc tggcccgcca tcctcgacga
    15301 gccgccccac ggaccgaact ccgtctgtgc cgtgacctcg acgccaacgt ccgcgccgcc
    15361 gccatcattc tggccaagcg agatgtgact gaccccggtc gcgcgatccg gcaacgtgac
    15421 cgccgtatag agcagcccgc cgctcgagct cggcgtctcg acacagatga tctcggcggt
    15481 gccgctgtag gttcccgctc cggccttctt tccagtgagc gtcacgtgga acgtgcccgc
    15541 gctcggtgct gcctcgtgcc attggatggt cagcagctcc gggatcagac ggctgccgaa
    15601 accgaggttc gccgcgggcc cggcggcgta catgtactgc aggatctccc gaaactcgat
    15661 ctcggcgacg acaaggacca ccgcggtctg ttcgttgccc tggccctgtg cggcctcggc
    15721 atttggcgtg tagtcaagct gcacctcgcc cacgttatcg gtgaacagcg ggccgatcgg
    15781 attcggcgtg agtgacccgt gcgcctcgat ggtcgccatc tgcgacgacc ggatgttcac
    15841 cggcagtcca tcgagcatcg tgcgatgggg caccaggagt ggcagctgga acatcggcga
    15901 a
//
LOCUS       DATBSW010000351         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_598422, whole genome shotgun sequence.
ACCESSION   DATBSW010000351 DATBSW010000000
VERSION     DATBSW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598422"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VIF63_09620"
     CDS             complement(<1..698)
                     /locus_tag="VIF63_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8239682.1"
                     /translation="MIASEALGQIETHPVDVLVVGAGGAGLRAAIEAQAAGAKVGLVC
                     KSLLGKAHTVMAEGGVAAALGHVDPEDKWQTHFRDTMVGGKLLNNPRMAQLHAMEAPD
                     RVRELELWGAVFDRTSDGRILQRPFGGHSHPRLAHVGDRTGLEMIRTLQDRAVASGID
                     VFMECTITKIVTGAAGVVGAFGYWRTTGAPVLFPAKAVVLATGGIGKAFAITSNSWEY
                     SGDGQALAYEAGAEL"
     gene            complement(695..1525)
                     /locus_tag="VIF63_09625"
     CDS             complement(695..1525)
                     /locus_tag="VIF63_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239683.1"
                     /translation="MTVQAAVLRALRKPRGTAQQGFGATNRRDRWAIYPIVQGLVFTI
                     CAGYLTFSGVLWGPLFGPQFVQVDGYLSPLFSPLILFPNMPTWLSPGLLILWIPIGFR
                     ATCYYYRKAYYRFYFADPPGCAVGEPTMHRRFALENALPFILQNLHRYLLYLAFIPLT
                     FLWLDILPAFNIVPDADVLDPRFRISALGLLFLVNVTLLTGYSLSCHSLRHLVGGRID
                     CFSCTAARRVRYTLWERLTDLNRNHMWWAWSSLITVTLADVYTRLLQAGVVSDFAFHL
                     "
     gene            complement(1522..2559)
                     /locus_tag="VIF63_09630"
     CDS             complement(1522..2559)
                     /locus_tag="VIF63_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEY8239684.1"
                     /translation="MAHVVTLIPGDGIGPELAEATRRVLDATGIAFDWEVQEAGEATI
                     ATEGTPLPERVLESIRRNKVALKGPITTPVGSGFRSVNVGLRQALNLYANLRPARSMV
                     GVQSRYEDVDLVIVRENTEDLYAGIEHMVGKDAAESIKIITRAASERIARFAFEYAIA
                     NGRRKVTAVHKANIMKLSDGLFLESCTTVAAGYAGRVEFEDRIVDNMCMQLVQKPDLY
                     DVLVLPNLYGDIVSDLCAGLVGGLGVAPGANIGEDGAVFEPVHGSAPKYAGQNKANPT
                     ALILSGALMLRHLGYAEMGERVEGAVRAVIAEGKTTTSDLGGTAGTREFADAVIDALG
                     SVAPAAASALA"
     gene            2614..3507
                     /locus_tag="VIF63_09635"
     CDS             2614..3507
                     /locus_tag="VIF63_09635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8239685.1"
                     /translation="MRIGAMVPLAYGDIEPGKAPSFAETMRFANVAESVGLDSIWVFD
                     HLIFRFPDEPEEGLNECWTTLSALAPVVPRVELGILVMCSSFRNAGLLAKMAGTLDEI
                     SQGRLILGLGCGWHEPEYDAFGFPFDHRVGRFEEDLEVTTRLLRGEKVDFDGKWSSYK
                     GAQLLPAPDRQPPILVASKGERMLDLTAKWADAWNTAWFGAVEDVVRERVADLDAACA
                     RMGRDPGAIRRTIGIRIREPGAAPDDGRSTDADAAGLADLFDEYAALGFADGIIWSLD
                     KTPAALERAGEARRIHLARHA"
     gene            3520..>4487
                     /gene="sucC"
                     /locus_tag="VIF63_09640"
     CDS             3520..>4487
                     /gene="sucC"
                     /locus_tag="VIF63_09640"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY8239686.1"
                     /translation="MKIQEADAKKLLLAQGLPVPPWEVARSPAEARAAASRLLHGAGH
                     VNQVVIKAQVLVGGRGKAGGVKLAGSDDEAEHVADAILGMSIKGITVRKVLVAPAADI
                     VKEFYLAAVLDRAARKILLMGSAEGGVEIEQVAVTNPEAIVRLHVDPLLGIRDWQARQ
                     LAFRLGLGKHLKATVAITKGLVRTMLANDADLVEINPLAIVREGGEERVVCLDAKITL
                     DDSALFRHPDHEALRDPDEEAPADAEARRHDISFIHLDGTIGCMVNGAGLAMTTMDLV
                     KRAGGEPANFLDIGGGAKAERVAAAMRLILAEPKVEAILVNIFGGIT"
BASE COUNT          716 a         1557 c         1570 g          644 t
ORIGIN      
        1 agctcggcgc ccgcttcgta ggcgagcgcc tggccgtcac cgctgtactc ccacgagttc
       61 gaggtgatcg cgaacgcctt gccgatcccc ccggtcgcca gcacgacggc cttggccggg
      121 aacaggaccg gcgcgccggt cgtgcgccag tagccgaagg cgccgacgac acccgccgcc
      181 ccggtgacga tcttcgtgat cgtgcattcc atgaacacgt cgatgcccga cgcgaccgcg
      241 cggtcctgga gtgtgcggat catctcgagg ccggtgcgat cgccgacgtg ggcgagccgc
      301 ggatgcgagt gcccgccgaa cggccgctgg aggatgcggc cgtcgctcgt gcggtcgaag
      361 acggcgcccc acagctcgag ctcccggacg cggtccgggg cttccatcgc gtgcagctgg
      421 gccatccgcg ggttgttgag cagcttgccg ccgaccatcg tgtcccggaa gtgggtctgc
      481 catttgtcct cgggatcgac atggccgagc gccgccgcga cgccaccctc ggccatgacc
      541 gtgtgggcct tgccgagcag cgacttgcac acgagcccga ccttcgcgcc tgctgcctgc
      601 gcctcgatcg cggcccgcag gccggcgcca ccggcgccga cgacgaggac gtcgacggga
      661 tgggtctcga tctggccgag cgcctccgac gcgatcacaa gtggaacgcg aagtcggaga
      721 ccacccctgc ctgcaggagg cgggtgtaga catcggcgag ggtgaccgtg atcaagctcg
      781 accaggccca ccacatgtgg ttgcggttga ggtccgtgag ccgttcccag agcgtgtatc
      841 gaacgcgccg ggcggcagtg cacgagaagc agtcgatccg gcccccgacg aggtggcgca
      901 gcgagtggca cgacagggag tagcccgtca gcagcgtgac gttgaccagg aacagcagac
      961 cgagcgcgct gatacgaaag cgcgggtcaa ggacgtcggc atcgggaacg atgttgaagg
     1021 ccggcaggat gtcgagccaa aggaaggtca gggggatgaa cgcgaggtac agcaggtacc
     1081 gatgcaggtt ctggaggatg aacggcagcg cgttctcgag cgcgaagcgg cggtgcatcg
     1141 tcggctcgcc gacggcgcag cccggtggat cggcgaagta gaagcggtag tacgccttcc
     1201 ggtagtagta gcaagtagcc cggaagccga tcgggatcca caagatcagg aggcctgggc
     1261 tcagccaggt cggcatgttc gggaagagga tcaacggcga aaacaggggc gagagatacc
     1321 cgtcgacctg cacgaattgc ggcccgaaga gcggccccca cagcaccccg ctgaacgtca
     1381 ggtagccggc gcagatcgtg aacacgaggc cctggacgat cggatagatc gcccatcggt
     1441 cgcggcggtt cgtcgccccg aagccctgct gcgcggtgcc ccgcggcttg cgcagcgccc
     1501 gcagcaccgc cgcctggacg gtcacgcgag ggcgctcgcg gcggcaggtg caacagaacc
     1561 aagggcgtcg atcaccgcgt ccgcaaactc gcgcgtcccg gcggtcccac cgaggtccga
     1621 cgtcgtcgtc ttgccctcgg cgatcacggc tcgcacggcg ccctcgaccc gctcgcccat
     1681 ctccgcgtaa ccaaggtggc ggagcatcaa agcacccgaa aggatgagcg cggtcgggtt
     1741 ggccttgttc tggccggcgt acttcggcgc ggagccgtgg accggctcga acaccgcgcc
     1801 gtcctcgccg atgttcgcgc cgggcgcgac cccgaggccg ccaaccaggc cggcgcacag
     1861 gtcggacacg atgtcgccgt acaggttcgg gagcaccagc acgtcgtaca ggtcgggctt
     1921 ctggacgagc tgcatgcaca tgttgtcgac gatccggtcc tcgaactcga cccggccggc
     1981 atagcccgcg gcaacggtcg tgcacgactc gaggaacagg ccgtcggaca gcttcatgat
     2041 gttggccttg tgcacggcgg tgaccttgcg ccggccgttc gcgatcgcgt actcgaaggc
     2101 aaagcgcgcg attcgttcgg aggcggcccg ggtgatgatc ttgatcgact ccgccgcatc
     2161 cttgccgacc atgtgctcga tgcccgcgta caggtcctcg gtgttctcgc gaacgatcac
     2221 gaggtcgacg tcctcgtacc ggctctggac gccgaccatc gaccgcgcag ggcgcaggtt
     2281 cgcgtacagg ttgagcgcct ggcggaggcc gacgttgacg ctccggaagc ctgacccgac
     2341 cggggtcgtg atcgggccct tcagcgccac cttgttgcgc cggatggact cgaggacccg
     2401 ctccggcagg ggcgtgccct cggtcgcgat cgtcgcctcg ccggcctcct ggacctccca
     2461 gtcgaaggcg attccggtgg cgtcgaggac gcggcgagtc gcctccgcca gctccgggcc
     2521 gatcccgtcg ccggggatga gggtcacgac atgggccatg cccgcattct aggcgcggac
     2581 ccgtgcgccc ggtccgcccc gtagactccg ccgatgcgca tcggagccat ggtcccgctc
     2641 gcctacggcg acatcgaacc ggggaaggcc ccgtccttcg ccgagacgat gaggttcgcc
     2701 aacgtcgccg agtcggtcgg gctggactcg atctgggtgt tcgaccacct gatcttccgc
     2761 ttccccgacg agcccgagga aggcctcaac gagtgctgga cgaccctctc ggcgctcgcc
     2821 cccgtggtgc ctcgggtcga gctcggcatc ctcgtcatgt gctcgtcgtt ccgcaacgcc
     2881 gggctgctcg cgaagatggc cggcacgctc gacgagatct cgcagggtcg cctgatcctc
     2941 ggcctcggct gcggctggca cgagcccgag tacgacgcgt tcggcttccc gttcgaccac
     3001 cgggtcggcc ggttcgagga ggatctcgag gtcacgacgc ggctcctgcg cggcgagaag
     3061 gtcgatttcg acgggaaatg gagctcgtac aagggtgccc agctgctccc cgccccggat
     3121 cggcagccgc cgatcctcgt cgcctcgaag ggcgagcgga tgctggacct gacggcgaaa
     3181 tgggccgacg cctggaacac ggcctggttc ggcgcggtgg aggacgtcgt ccgcgagcgc
     3241 gtcgcggacc tggacgctgc ctgcgcgcgc atgggccgtg atccgggcgc gatccggcgg
     3301 acgatcggga tccggatccg ggaacccggc gcggcccccg acgacgggcg cagcacggac
     3361 gccgacgcgg cggggctcgc cgacctgttc gacgagtacg cggcgcttgg tttcgcggac
     3421 gggatcatct ggtcgctgga caagacgccc gccgccctcg agcgcgccgg cgaggcgcgg
     3481 cgtatccacc tggcccgaca cgcctaggat tcagcggccg tgaagatcca ggaagccgac
     3541 gccaagaagc tgctgctcgc ccagggcctg cccgtgccgc cgtgggaggt ggcgcgcagc
     3601 cccgccgagg cccgcgccgc ggcgtcccgc ctcctgcacg gggccggcca cgtgaaccag
     3661 gtcgtgatca aggcccaggt cctcgtcggc ggccggggca aggccggcgg cgtcaagctc
     3721 gccggctccg acgacgaggc ggagcatgtc gccgacgcga tcctcggcat gtcgatcaag
     3781 ggcatcacgg tccggaaggt gctcgtggcg ccggcggcgg acatcgtcaa ggagttctac
     3841 ctcgcggcgg tgctggatcg ggccgcgcgg aagatcctgc tcatgggctc ggccgagggc
     3901 ggcgtcgaga tcgagcaagt ggccgtcacg aaccccgagg cgatcgtccg cctccacgtc
     3961 gatcccctgc tcgggatccg tgactggcag gcccgccagc tggcgttccg cctgggtctc
     4021 ggcaagcacc tcaaggcgac cgtcgcgatc acgaagggcc tcgtccgcac gatgctcgcg
     4081 aacgacgccg acctcgtcga gatcaacccg ctcgcgatcg tccgcgaggg cggcgaggag
     4141 cgcgtcgtct gcctcgacgc caagatcacc ctcgacgact cggccctctt ccgccacccc
     4201 gatcacgagg cgctgcgcga ccccgacgaa gaagcgcccg cggacgccga ggcgcgccgc
     4261 cacgacatca gcttcatcca cctcgacggc acgatcggct gcatggtcaa cggcgccggc
     4321 cttgcgatga cgaccatgga cctggtcaag cgcgccggcg gcgagcccgc caacttcctc
     4381 gacatcggcg gcggcgccaa ggccgagcga gttgccgcgg cgatgcgcct gatcctggcc
     4441 gagcccaagg tcgaggcgat cctcgtgaac atcttcggcg gcatcac
//
LOCUS       DATBSW010000352         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_879988, whole genome shotgun sequence.
ACCESSION   DATBSW010000352 DATBSW010000000
VERSION     DATBSW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879988"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1192
                     /locus_tag="VIF63_09645"
     CDS             203..1192
                     /locus_tag="VIF63_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY8239687.1"
                     /translation="MQFGFTLKPEHSIQDTIDLAKLGESVGFEYLWLFDSHVLWRDPY
                     PLLTLIAEHTSKLRLGTCVTNPGTREPSVTASSLATLDELSGGRMDLGIGRGDSARRV
                     LGKPPITMAGTEEAISVIRDLVEGRAVTYEGTELNLTWTGKWQLPVWVAGYGPMALAM
                     TGRIADGVILQLADPDLIRWFGGQVRDAAKAAGRDPASIRIQAAAPLHIGPRELGRER
                     TRWFPALVSNHVVDLVNKYPREQLPDSLTGYVQDRTGYDYHHHAEVGSSNAGFVGDEV
                     TDRFCVLGEVAEVVDKLHVLADAGVDQFNVYLMNGDERAQLEQIGREVIPALG"
     gene            1434..1643
                     /locus_tag="VIF63_09650"
     CDS             1434..1643
                     /locus_tag="VIF63_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239688.1"
                     /translation="MPTILAFRRRDPWWGQEGPAARRKHRYERFVSTTAFVASLFALG
                     GAAFVWAVHLGFAAMLGIRVTLAIG"
     gene            1750..>2448
                     /locus_tag="VIF63_09655"
     CDS             1750..>2448
                     /locus_tag="VIF63_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8239689.1"
                     /translation="MRTLISNGTIVTADGSYAGDILVDGEQIAAVGAGIGAMAQADET
                     IDASGKYVIPGGIDVHTHMELPFGGTFAKDTFETGTRAAAFGGTTSIVDFAVQSRGKS
                     LREGLDAWHAKAEGNAVTDYGFHMIMSDVNDDTLAEMDGLVEEGVPDFKLFTAYPGVF
                     YSDDGAIFRAMQQTGKNGGLIMMHAENGMAIDIVAADLVADGKTDPLYHGIARYPVFE
                     GEATNRVIRLAEAAQ"
BASE COUNT          416 a          866 c          809 g          357 t
ORIGIN      
        1 gtcctcgagg gcgcgctcga gctccacggc gcgcacggag tagaacgggt tggtccaggc
       61 gatgtcctgt tcatcccgcc cgacaccgag cacggcctgc gtacgcccaa aggcggccgc
      121 tggctggcga tctggcccgt tggggagcac gtcccgggcc cgcgctacgg ctaatctcac
      181 cggacacgca gggagcgcga gcatgcagtt cggattcacg ctcaagccgg agcactcgat
      241 ccaggacacg atcgacctgg cgaagctggg cgagtccgtc gggttcgagt atctgtggct
      301 cttcgactcg cacgtcctgt ggcgagaccc gtatcccctc ctgacgctca ttgccgagca
      361 cacgtcgaag ctccggctcg gcacctgcgt gaccaacccg gggacgcgcg agccgtccgt
      421 caccgcctcc agcctggcga cgctcgacga gctgagcggc ggccgcatgg acctgggcat
      481 cggccgcggc gactcggccc ggcgcgtgct cggcaagcca ccgatcacga tggccggcac
      541 ggaggaggcg atcagcgtca tccgcgacct cgtcgaaggc cgggcggtga cgtacgaagg
      601 aaccgagctc aacctcacat ggacgggcaa gtggcagctg ccggtgtggg tggcgggcta
      661 cggcccgatg gcgctcgcga tgaccggccg tatcgccgac ggcgtgatcc tccagctcgc
      721 cgatcccgac ctcatccgct ggttcggcgg ccaggtccga gacgccgcga aggccgccgg
      781 gcgcgacccg gcgtcgatca ggatccaggc cgcggcaccg ctccacatcg gaccgcgcga
      841 gctgggccgc gagcgcacgc gctggttccc ggcgctggtc tcgaaccacg tcgtcgatct
      901 cgtgaacaag tacccgcgcg agcagcttcc cgattcactc accggctacg tccaggaccg
      961 gacgggctac gactaccacc accatgccga ggtcggatca tcgaatgccg gcttcgtcgg
     1021 cgacgaggtg accgatcgct tctgcgtcct gggcgaggtc gcggaggtgg tcgacaagct
     1081 gcacgtcctg gccgacgccg gggtggacca gttcaacgtc tacctgatga acggcgacga
     1141 acgcgcccag ctcgagcaga tcgggcgcga ggtgatcccg gcgctcgggt aggatcgtcc
     1201 ctgcaggcac cacccatcca accagctgga ggttgacgtg gacaagaagg caaagacccc
     1261 caagaagccc aagcaggcca agaccaagac cgccaagtag cgggcgttcg gcgggcgctg
     1321 ggacattcgt cgccggcgcc cgaaccgaag tcaccctttc ctcctgaccg caccggaccg
     1381 acactggcga tcccttcgct cgcatccccg gccagcagca gtcgaggcgt ctcatgccga
     1441 ccatcctggc ctttcgtcgc cgtgatccgt ggtggggcca ggaaggtccg gccgcgcgac
     1501 gcaagcaccg ctacgaacgc ttcgtctcca cgacggcgtt cgtcgcatcg ctcttcgccc
     1561 tcggcggcgc cgccttcgtg tgggccgtcc acctgggctt cgccgcgatg ctcgggatcc
     1621 gggtgacgct cgccatcggc tgaggccgcc atcggccaag ccgctaacgg ctgaggcctc
     1681 gccgcgctcg gtgcggctcg ctacgatctc cttcgacggc ctcggccggc gcccatcgaa
     1741 ggaggaacga tgcgcaccct gatctcgaat ggcacgatcg tcacggccga cggctcctac
     1801 gcgggcgaca tcctcgtcga cggcgagcag atcgcggccg tcggcgccgg catcggggcg
     1861 atggcccagg cggacgagac catcgacgcc tcgggcaagt acgtgatccc cggcggcatc
     1921 gacgtccaca cccacatgga gctgccgttc ggcggcacgt tcgccaagga cacgtttgag
     1981 acgggcaccc gcgccgcggc cttcggcggc acgacgagca tcgtcgactt cgccgtccag
     2041 tcacgcggca agagcctgcg cgaaggcctc gatgcctggc acgccaaggc cgagggcaat
     2101 gccgtcaccg actacggctt ccacatgatc atgagcgacg tcaacgacga cacgctcgcc
     2161 gagatggacg gtctcgtcga ggagggcgtg cccgacttca agctcttcac cgcctacccc
     2221 ggcgtgttct acagcgacga cggggcgatc ttccgggcga tgcagcagac cggcaagaac
     2281 ggcgggctga tcatgatgca cgccgagaac ggcatggcca ttgacatcgt cgccgccgat
     2341 ctagtggccg atggcaagac ggatccgctc tatcacggga tcgcccggta tccggtcttc
     2401 gagggcgagg cgaccaatcg cgtcatcagg cttgccgaag ccgcccag
//
LOCUS       DATBSW010000353         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_633655, whole genome shotgun sequence.
ACCESSION   DATBSW010000353 DATBSW010000000
VERSION     DATBSW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633655"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VIF63_09660"
     CDS             complement(<1..480)
                     /locus_tag="VIF63_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8239690.1"
                     /translation="MLPRPPGRSGRPPGTQKSDPSGPARWLELSVEADGEAVEAVSEI
                     LARVAPAGTSVEPGFQLIEDGLGARIDPNLPATIRAYVPAIDEAAATRAVATVTEALG
                     HLQAFNLRPIGELHTRVVDEADWAEEWKEHFHVLRIGRRLVIKPSWRRHRRADDEVVI
                     "
     gene            complement(482..1582)
                     /gene="dnaJ"
                     /locus_tag="VIF63_09665"
     CDS             complement(482..1582)
                     /gene="dnaJ"
                     /locus_tag="VIF63_09665"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY8239691.1"
                     /translation="MAIGDYYEILGVVRAASEADIKKAYRKLAQRWHPDVNQEPEAAQ
                     KFKEISEAYQVLSDPERRQRYDLFGTVGAEGGPGAGFGGFSDIFDAFFGAAAAGTGRR
                     GRPASGSDLRYDLRITFEEAILGTQKEIEFPVLSRCETCGGNGAKVGTQPITCPQCNG
                     RGEIRGVRNTMLGQMINVTTCPKCRGDGRIVETPCETCRGEGRIERKRTLQVTIPPGI
                     DEGHQIRLSNEGEAGPRGGIAGSLYVAVHVTPHATLRRDGTELVYEASVGLAQAALGT
                     TILVPTVEGEESVEVKAGTQPGTEIRLRGKGVPYLRRPGQRGDLHVLVDVEIPSRLSR
                     AQREALEAYADATGEIVNGPNSFLDRVRGKKA"
     gene            1678..2529
                     /locus_tag="VIF63_09670"
     CDS             1678..2529
                     /locus_tag="VIF63_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY8239692.1"
                     /translation="MTTDADQPRLTVALTFDHDSLSDGIRRGDPPIKMSHAEFGHRVG
                     VPRILELLEREGIKSSWFIPGATLELYPSDTEAILAGGHEVGCHGWFHEDYSVLDAAE
                     QRAIQDRSIDAVERFTGQRPRGHRAPYWAFSDDTIGVLEDAGFAYDSSLMADDYRPYR
                     VRRGDRHSPSDGSTFGPESSLIEVPVYWTLDDWPLFEPGPDNGRDGMSAPSKVLEIWK
                     AELRYAWEHAPGGLLTITMHPECIGRGHRMAMVEQFIAEAKALEGVVFDRLDGYLARL
                     RLVDVAG"
     gene            complement(2596..3150)
                     /locus_tag="VIF63_09675"
     CDS             complement(2596..3150)
                     /locus_tag="VIF63_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239693.1"
                     /translation="MSVVGSLRICALFLGVALVAACGGGTAGTPQPAGYPLNACPVVG
                     AGAITASSGYQLVTPGEIAFGWKEWHNETPAGSACGAQSVKGAELTIHRGAIVGNVEV
                     NFVATLNRVAPLVAPDPVSPRLQLFRIDDAGPTLLETVPIQLDEQYKAMGAVLPGAPV
                     GRYRVTILSQANEVLADGTFTIVE"
     gene            complement(3147..3854)
                     /locus_tag="VIF63_09680"
     CDS             complement(3147..3854)
                     /locus_tag="VIF63_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8239694.1"
                     /translation="MSAGTNDRAAALAAIEREVIDCRRCPRLVEWRELVAHEKVARFR
                     HDEYWGRPVPGFGDPDARILLLGLAPAAHGGNRTGRVFTGDGSGDFLWPALHTAGLAD
                     RPTSERRGDGLRAIDLWVTAPVKCAPPANKPLPSERGNCAPYLVRELAALDRVRVVVA
                     LGAFAWDSALSTFEALGHAPLRPKPKFGHGAEVNIGPWTLLGSYHVSQQNTFTGRLTH
                     AMLDAIFVRALALSSAV"
     gene            complement(3851..4150)
                     /locus_tag="VIF63_09685"
     CDS             complement(3851..4150)
                     /locus_tag="VIF63_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239695.1"
                     /translation="MTRRDVWLSIAVSVLSLVILDYILPTFGVLIGLFLFDAVLRVWY
                     VGVALVVVSFVLTRRRAVPTAARVVGCIGVWWLTFVVSYIVILVGMFAVFGGLTG"
BASE COUNT          620 a         1497 c         1470 g          636 t
ORIGIN      
        1 gatgaccacc tcgtcgtcgg cgcggcggtg gcgacgccag ctcggcttga tgacgaggcg
       61 gcggccgatc cgcaggacgt ggaagtgctc cttccattcc tcggcccagt cggcctcgtc
      121 gacgacacgt gtgtgaagct cgccgatcgg gcgcaggttg aatgcctgga gatggccgag
      181 ggcttcggtg accgttgcga cggcacgcgt cgcggctgct tcatcgatgg ccggcacgta
      241 ggcgcgaatc gtcgccggca ggttcgggtc gatccttgcg ccgagcccat cctcgatcag
      301 ctggaagccc ggttcgacgc ttgtccctgc cggcgcaacc cgggcgagga tctcgctgac
      361 cgcctcgacg gcctcgccgt ccgcctcgac cgacagctcc agccagcggg ctgggccgct
      421 cgggtcggac ttctgtgtcc ccggcgggcg gcccgatcgc ccaggtgggc gcggcagcac
      481 cctaggcttt cttgccgcgc acgcgatcca ggaagctgtt cggtccgttc acgatctcgc
      541 cggtcgcgtc ggcgtacgcc tcgagcgcct cgcgctgtgc gcgcgagagc cggctcggga
      601 tctcgacatc gacgagcacg tggaggtcgc cgcgctgccc gggcctgcgc aggtacggca
      661 cgcccttgcc gcggagccgg atctcggtgc cgggctgggt gcctgccttc acctcgaccg
      721 attcctcgcc ctcgaccgtc ggcaccagga tcgtcgtgcc cagcgccgcc tgggcgagcc
      781 cgaccgaggc ctcgtagacc agctccgtcc catcgcggcg cagggtcgcg tgcggcgtga
      841 cgtggaccgc gacgtagagc gagccggcga tgcccccacg cggacctgcc tcgccttcgt
      901 tggagagccg gatctggtgg ccctcgtcga tgcccggcgg gatcgtgacc tgaagcgtgc
      961 gcttgcgctc gatccggccc tcgccccggc acgtctcgca cggtgtctcg acgatccgcc
     1021 cgtcgccgcg gcacttcggg caggtcgtga cgttgatcat ctggccgagc atcgtgttgc
     1081 ggacgccgcg gatctcgccc cggccgttgc actgcgggca ggtgatcggc tgggtgccca
     1141 ccttcgcgcc gttgccgccg cacgtctcgc agcgcgacag gaccggaaac tcgatctcct
     1201 tctgcgtccc gaggatcgcc tcctcgaacg tgatccggag gtcgtagcgc aggtccgagc
     1261 ccgacgccgg acgcccccgc cggcccgttc ccgcggctgc cgccccgaag aacgcgtcga
     1321 agatgtcgct gaagccaccg aagccggcgc ccggtccgcc ttccgcgccc acggtcccga
     1381 acaggtcgta gcgctggcgc cgctccgggt cggagaggac ctggtaggcc tcgctgatct
     1441 ccttgaactt ctgtgcggct tcgggctcct ggttgacgtc cgggtgccag cgctgggcga
     1501 gcttccggta cgccttcttg atatcggcct cggacgccgc gcgcaccacg ccgaggatct
     1561 cgtagtaatc gccgatcgcc atccctcgaa gaatacgggt gaattcggcg cggcgagggg
     1621 cacgacccgg gtgcgacacg gggcgcgacg cgggaggctc gccgtaccat cgcggcgatg
     1681 accaccgatg ccgaccagcc acgcctgacc gtcgccctga cgttcgacca cgattcgctt
     1741 tcggacggca tccgccgtgg cgaccccccg atcaagatgt cccatgccga gttcggccac
     1801 cgggtgggcg tcccgcgcat cctcgagctg ctcgagcgcg aagggatcaa gtcgtcgtgg
     1861 ttcatccccg gcgcgacgct cgagctctat cccagcgaca ccgaggcgat cctcgccggc
     1921 ggccacgagg tcggctgcca cggctggttc cacgaggatt actccgtcct cgacgcagcg
     1981 gagcagcgcg cgatccagga ccgctcgatc gatgcggtcg agcggttcac gggccagcga
     2041 cctcgtggcc accgcgcgcc gtactgggcg ttcagcgacg acacgatcgg agtcctcgag
     2101 gacgccggct tcgcctacga ctcgtcgctc atggccgacg actaccggcc gtaccgggtg
     2161 cgccggggcg atcgacactc gccgtccgat ggctcgacgt tcgggccgga gagcagcctc
     2221 atcgaagtgc cggtctactg gacgctcgac gactggccgc tcttcgagcc gggcccggat
     2281 aacggccgcg atgggatgtc cgcgccgtcg aaggtgctcg agatctggaa ggctgagctg
     2341 cgctacgcgt gggagcacgc gcccggcggg ctgctgacga tcacgatgca ccccgagtgc
     2401 atcgggcgcg gccatcgaat ggccatggtc gagcagttca tcgccgaggc gaaggccctc
     2461 gaaggcgtgg tgttcgaccg gctcgacggc tacctcgcgc gacttcgact ggtcgacgtc
     2521 gccggctgag cggcctgctc gcggcgccag tcgccgctcc ggtcgccgcg ggggttgccg
     2581 cgccggtcgc cgcgcctact cgacgatcgt gaacgtcccg tcggcgagga cctcgttcgc
     2641 ctgcgagagg atcgtcaccc ggtagcgccc gaccggggcg cctggcagga ccgcgcccat
     2701 cgccttgtac tgctcatcga gctggatcgg cacggtctcg aggagggtgg gaccggcgtc
     2761 gtcgatccgg aagagctgca ggcggggtga aacgggatcg ggcgccacga gcggcgcgac
     2821 gcgattgagg gtggcgacga agttcacctc gacgttgccg acgatcgcgc ctcggtgaat
     2881 cgtgagctcg gcgcctttca ccgattgcgc cccgcaggca ctcccagccg gcgtctcgtt
     2941 gtgccattcc ttccagccga acgcaatctc gccgggcgtg acgagctggt agccactgct
     3001 cgcggtgatc gcgccggcgc caacgaccgg gcaggcgttg agcgggtagc cggcaggttg
     3061 cggggtgccc gctgttccgc cgccgcacgc cgcgaccaac gcaacgccga ggaagagggc
     3121 gcagatccga agcgacccga ccacgctcac accgctgacg atagcgcgag cgcccgcacg
     3181 aagatcgcgt ccagcatcgc gtgggtgagg cgacccgtga acgtgttctg ctggctgacg
     3241 tggtagctcc cgagcagcgt ccacgggccg atgttcacct ccgcgccgtg gccgaatttc
     3301 ggcttgggtc gcaggggcgc gtggcccagc gcttcgaacg tgctcagggc cgagtcccag
     3361 gcgaaggcgc cgagggcgac gacgacacgc acgcggtcga gcgcggccag ctcccgcacg
     3421 aggtacggcg cgcagttgcc gcgctccgag gggagcggct tgttggccgg cggcgcgcac
     3481 ttcaccgggg ccgtcaccca gaggtcgatt gcgcggaggc cgtcgccgcg gcgctcactc
     3541 gtcgggcggt cggcgaggcc agccgtgtgg agcgccggcc acaggaagtc gccgctgccg
     3601 tcgccggtga acacgcggcc ggtgcggttg ccgccgtgcg ccgcgggcgc gaggccgagc
     3661 agcaggattc gcgcgtcagg gtcgccgaac ccgggcacgg gcctgcccca gtactcgtcg
     3721 tggcggaagc gcgcgacctt ctcgtgcgcg acgagctcgc gccactcgac aaggcgcggg
     3781 caacgccggc aatcgatgac ctcgcgttcg atcgccgcga gcgctgctgc gcgatcgttc
     3841 gtgcccgcgc tcaacccgtg aggcccccga acacggcgaa catgcccacc aggatgacga
     3901 tgtagctcac gacgaacgtg agccaccaga cgccgatgca cccgacgacc ctggcggcgg
     3961 ttggcaccgc gcgacgtcgc gtgaggacaa atgacacaac caccagggca acgccgacgt
     4021 accacacccg gagaacggca tcaaacagaa acagtcctat cagtaccccg aaagtcggca
     4081 ggatgtagtc gaggatcacc agcgacagga cgctgacggc gatgctcagc catacatccc
     4141 ggcgggtcat tcgtcgagcc cccggccttc ggcgctgcta cttgaggagc agcttgtcga
     4201 gccgtcgcga gacgatcgcg agc
//
LOCUS       DATBSW010000354         7050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1198031, whole genome shotgun sequence.
ACCESSION   DATBSW010000354 DATBSW010000000
VERSION     DATBSW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198031"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1742
                     /locus_tag="VIF63_09690"
     CDS             60..1742
                     /locus_tag="VIF63_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8239696.1"
                     /translation="MIASEALGHVDPEDKWQTHFRDTMVGGKLLNNPRMAQLHAMEAP
                     DRVRELELWGAVFDRTTDGRILQRPFGGHSHPRLAHVGDRTGLEMIRTLQDRAVASGI
                     DVFMECTITKIITGAVGVVGAFGYWRTTGAPVLFPAKAVVLATGGIGKAFAITSNSWE
                     YSGDGQALAYEAGAELIDMEFVQFHPTGMVWPPGVRGLLVTEAVRGEGGILRNKDGER
                     FMWKYLPEDRRGEYAATDEEAARWVAAASRGQTSDARRPPELSTRDNVSRAIYTEVRE
                     GRGTEHGGVFLDISYLPVEHVRRKLPSMYDQFKELADVDITKGPMEVGPTTHYVMGGI
                     RVEADTGATTVPGLFAAGEVAGGMHGANRLGGNSLSDLLVFGQRTGAGAAAHAAGHND
                     QAYVDPVQVRDAARELAAPLERAAAAAAGADAEDPYALQRDLQEMMQRLVGIFRVQAD
                     LEEAIGELAKLRARAPRLAAGGGRVFNPGWNLVFEVRNLLVVAEAITRSALQRTESRG
                     AHARLDFPETDQPGWGGRNSVVRRRQDGSMEVATTTLPPMPADLGELLGSAH"
     gene            1768..2763
                     /locus_tag="VIF63_09695"
     CDS             1768..2763
                     /locus_tag="VIF63_09695"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY8239697.1"
                     /translation="MTEAHLKVFRGEPGGATRFDEFDVPVEEGMVVLDALHWIQGNAA
                     PDLAVRWNCKAAKCGSCSAEVNGRPRLTCKTRLSDFEPGEEITVEPMRAFPLVRDLVT
                     DVSWNYEVNKSIQPFTPPAGVPQSDWRWQQQDIERVQEYRKCIECFLCQDVCHVLRNH
                     ETEKPFMGPRFLVRTAGLEMHPIDEGDRRGYLKDTGNIGYCNITKCCTEVCPEHIKIT
                     DNAIIPLKERVADEFYDPVMWAWRKLRGKSSEAPSRPPLPMLHGVAATDTSTAAPSET
                     APAAMAAEPMAEGATAVAEVGPPRAVAKRARKTATARPKPAKRRSDAGDETVSEG"
     gene            2774..3391
                     /locus_tag="VIF63_09700"
     CDS             2774..3391
                     /locus_tag="VIF63_09700"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEY8239698.1"
                     /translation="MREPPLTFGFDDEPDADERTSDDEPTPGGPRPRGPGAKSRSSKP
                     GADETASKGFVVRTDGAARGNPGPASAGAVLLSLSRPDARNPRAVPDASISDYLGVQT
                     NNVAEYTAVVRAVALGLELGARRLELLLDSKLIVEQITGRWRVKDAKLRVLWAEVLTL
                     LRQLPDGWTAAHVPRAQNSLADAMCNEAIDRVMAGGPDSVVSRPT"
     gene            complement(3408..3887)
                     /locus_tag="VIF63_09705"
     CDS             complement(3408..3887)
                     /locus_tag="VIF63_09705"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8239699.1"
                     /translation="MTDTTAAAASRRTVTLSRHFDASPSEVYRAWTDPEEVAQWYGPD
                     GWYAPADRSRIDTTAGGRWEVTMIRRSDGFEFPIGYEILELVEPLLVVLRHTEQDPGG
                     SGATIVRVELTADGEGTLMTLSDGPMETYGADSAESGYQQAFEKLAARLGMGRSESV"
     gene            complement(3884..4228)
                     /locus_tag="VIF63_09710"
     CDS             complement(3884..4228)
                     /locus_tag="VIF63_09710"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8239700.1"
                     /translation="MPADQLHQLDQTFAALADPIRRSILARLAEGSATVNELASPFPV
                     SLPAISRHLKVLERAGLIVREREAQWRPSRLAPAPLLEARGWLEARTRAWERRIDRLD
                     THLQHKQRDRRP"
     gene            4324..4672
                     /gene="rnpB"
                     /locus_tag="VIF63_09715"
     ncRNA           4324..4672
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIF63_09715"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4740..4934
                     /locus_tag="VIF63_09720"
     CDS             4740..4934
                     /locus_tag="VIF63_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239701.1"
                     /translation="MLNEVSFRLMHRHGDDYEPMVEHTSADHDPEKGWLRGARIFRCR
                     SCEEEVVLVPPTNEPGDTSA"
     gene            complement(4951..5196)
                     /locus_tag="VIF63_09725"
     CDS             complement(4951..5196)
                     /locus_tag="VIF63_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239702.1"
                     /translation="MALPHVIESVMLPDPSHLAPHRIKRCTFRRLIRVDPRGERRYDV
                     ECLYPDRKLAIPLGDLDAATPICNACTAAHIFRPDED"
     gene            5342..6067
                     /locus_tag="VIF63_09730"
     CDS             5342..6067
                     /locus_tag="VIF63_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY8239703.1"
                     /translation="MDGSIREPAQRTLTPAVLASAAFVAICAAFAITYVAARGGLQMP
                     VAPIGSAAAVASPAPTTDQPTQPAPSIAPVPTPLPSPSQPPPSATPEPTPEPTVEPTP
                     GPTLVIPTLRPNDPLADLRACPGHPGCYRYVVQRLDTLSTIADRFLVPMGTILALNPQ
                     ITDPSVVVTNSTMYLGRSRFVRLDPCPDGALCWLYVVRAGDSLAGIANRYALTRDAIL
                     EINPGLPRPLTIDQVIRLPDVPA"
     gene            6328..6777
                     /gene="mraZ"
                     /locus_tag="VIF63_09735"
     CDS             6328..6777
                     /gene="mraZ"
                     /locus_tag="VIF63_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY8239704.1"
                     /translation="MGNGVRWVFTGEYRHTVDDKGRLAIPARFRAQLEGGVVLSRWID
                     GCLAIHTRAGWDALSAKVAGVALTDPAGRLFERAIFAGALELEMDKQGRVLVASYLRD
                     EVGITADALVIGVRDHGEIWAPDRWQSYRRAMDDPAKFAEAIRGLGI"
     gene            6783..>7050
                     /gene="mraW"
                     /locus_tag="VIF63_09740"
     CDS             6783..>7050
                     /gene="mraW"
                     /locus_tag="VIF63_09740"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY8239705.1"
                     /translation="MTDELPTVHVPVMADEVVEMLAPVAGSRHIDATLGGGGHAERIL
                     EASDPDGRLLGLDADGAAIARVRARLAPRFGPRLDLRQANFRELA"
BASE COUNT         1109 a         2533 c         2434 g          974 t
ORIGIN      
        1 gccgatgtct acacccgcct cctgcaggca ggggtggtct ccgacttcgc gttccacttg
       61 tgatcgcgtc ggaggcgctc ggccatgtcg accccgagga caaatggcag acccacttcc
      121 gggacaccat ggtcggcggc aagctgctca acaacccgcg gatggcgcag ctgcacgcga
      181 tggaggcccc ggaccgcgtc cgggagctcg agctgtgggg cgccgtcttc gaccggacga
      241 ccgacggccg catcctccag cgcccgttcg gcggccactc gcatccgcgc ctcgcccacg
      301 tcggcgaccg caccggcctc gagatgatcc gcacgctcca ggatcgcgcg gtcgcgtccg
      361 ggatcgacgt gttcatggaa tgcacgatca cgaagatcat caccggggcg gtcggggtcg
      421 tcggtgcctt cggctactgg cgcacgaccg gcgcgccggt gctgttcccg gccaaggccg
      481 tcgtgctggc gaccggcgga atcggcaagg cgttcgcgat cacctcgaac tcgtgggagt
      541 acagcggtga cggccaggcc ctcgcctacg aggccggcgc cgagctcatc gacatggagt
      601 tcgtccagtt ccatcccacc ggcatggtct ggccgccggg cgtgcgcggc ctgctcgtca
      661 ccgaggcggt gcgcggcgag ggcggcatcc tgcgcaacaa ggacggcgag cggttcatgt
      721 ggaagtacct gcccgaggat cgccggggcg agtacgccgc aaccgacgag gaagccgccc
      781 gctgggtcgc cgccgcgtca cgcggccaga cgagcgacgc gcgccggcca cccgagcttt
      841 ccactcggga caatgtctcg cgcgccatct acaccgaagt ccgtgaaggc cgcggcaccg
      901 agcacggcgg cgtgttcctg gacatcagct acctgcccgt cgagcatgtg cggcgaaagc
      961 tcccgtcgat gtacgaccag ttcaaggagc tcgccgacgt cgacatcacg aaaggcccga
     1021 tggaggtcgg cccgacgacc cactacgtca tgggcggcat ccgcgtcgag gcggacaccg
     1081 gcgcgacgac cgttcccggg ctgttcgccg cgggcgaggt cgccggcggg atgcatggtg
     1141 cgaaccggct cggtggcaac tcgctgtccg acctgctcgt gttcggccag cgaacgggcg
     1201 ccggcgccgc ggcccacgcg gcaggccaca acgaccaggc gtacgtcgat cccgtgcagg
     1261 tgcgcgacgc ggcccgtgag ctcgcggcgc cactcgagcg cgcagctgct gcggccgcgg
     1321 gcgccgacgc tgaggacccg tacgcgctgc agcgcgatct ccaggaaatg atgcagcgac
     1381 tggtcggcat cttccgggtc caagccgacc tggaggaggc gatcggcgag ctcgccaagc
     1441 ttcgggcgcg ggccccgcgc ctcgccgccg gcgggggacg ggtcttcaac ccgggctgga
     1501 acctcgtctt cgaggtgcgc aacctgctcg tcgtcgccga ggcgatcacg cgcagcgcgc
     1561 tccagcgaac cgagagtcgc ggcgcccacg cccgcctcga cttccccgag accgaccagc
     1621 cgggctgggg tggtcgaaac agcgtcgtca ggcgccgcca ggacggctcg atggaggtcg
     1681 cgaccacgac gctcccgccc atgcccgccg acctgggcga gctcctcggg agcgcacact
     1741 gatggccacg atgaccggga gcgatcgatg accgaggccc acctgaaggt cttccggggc
     1801 gagccgggcg gggcgacgcg cttcgacgag ttcgacgtgc cggtggagga gggcatggtc
     1861 gtcctcgacg ccctccactg gatccagggc aacgccgcgc ccgacctggc cgtgcgctgg
     1921 aactgcaagg cagccaagtg cggctcctgc agcgccgagg tcaacggccg gccgcgcctg
     1981 acgtgcaaga cccgcctgtc cgacttcgag cctggcgagg agatcacggt cgagccgatg
     2041 cgcgcgttcc cgctggtgcg cgacctcgtc acggacgtct catggaacta cgaggtcaac
     2101 aagtcgatcc agccgttcac gccgccggcg ggggtgccgc agtcggactg gcgctggcag
     2161 cagcaggaca tcgagcgggt ccaggagtac cggaagtgca tcgagtgctt cctgtgccag
     2221 gacgtctgcc acgtcctgcg caaccacgag accgagaagc cgttcatggg tccgcgcttc
     2281 ctcgtccgga ccgccggcct cgagatgcac ccgatcgacg agggcgaccg gcgcggctac
     2341 ctcaaggaca ccggcaacat cggctactgc aacatcacga agtgctgcac cgaggtctgc
     2401 ccggagcaca tcaagatcac cgacaacgcg atcatcccgc tcaaggagcg cgtcgccgac
     2461 gagttctacg acccggtgat gtgggcctgg cgcaagctcc ggggcaagtc gtcggaggcg
     2521 ccgtccagac caccgctgcc gatgctccac ggcgtcgccg cgacagacac gtcgacggcc
     2581 gctccgtcgg agacggctcc cgccgcgatg gcggccgagc cgatggccga gggcgcgacg
     2641 gccgtcgccg aggtgggtcc gccacgagcg gtcgccaagc gcgcgcgcaa gaccgcgacg
     2701 gcgcgaccga agcccgccaa gcgccgctcc gacgcgggcg acgagacggt ctcggaaggc
     2761 tgatcccgcc cgggtgcgcg aaccgccgct gacattcggg ttcgacgacg agccggacgc
     2821 cgacgaacgc acatccgacg acgaaccgac tcccggcggg ccgcgtcctc gcggccccgg
     2881 cgccaagtcg cggtcatcca agcccggcgc cgacgagaca gcgagcaagg gcttcgtcgt
     2941 tcgcacggac ggcgccgctc gcggcaaccc cggtccggcg tctgccggag cggtgctgct
     3001 gtccctgtcc cggcccgacg ccagaaaccc ccgcgccgtc ccggacgcct cgatctccga
     3061 ctacctcggc gtccagacca acaacgtggc cgagtacacg gcggtcgttc gcgcggtcgc
     3121 gctgggcctc gagctcggcg cccggcgcct ggagctgctc ctggactcga agctcatcgt
     3181 cgagcagatc accggccgct ggcgcgtcaa ggacgcgaag ctccgcgtcc tgtgggccga
     3241 ggtcctgacg ctcctccggc agctgcccga cggctggacc gcggcgcacg tgccccgcgc
     3301 ccagaactcc ctggccgacg cgatgtgcaa cgaggctatc gaccgcgtga tggccggcgg
     3361 ccccgactcg gtcgtcagtc gcccaacgta gcgagcgcgt cgggcgatca gacagactca
     3421 gagcgaccca tgccgaggcg cgctgcgagc ttctcgaatg cctgctggta gccgctctcg
     3481 gcgctatcgg cgccgtacgt ttccatggga ccatcgctca gcgtcatcag cgtgccctcg
     3541 ccatcggcgg tgagctcgac gcggacgatc gttgcgccgc tgccgccggg gtcctgctcg
     3601 gtgtggcgaa ggaccaccag taacggctcg acgagctcga ggatctcata cccgatcggg
     3661 aactcgaagc cgtccgagcg gcggatcatc gtgacctccc agcgtccgcc ggcagtggtg
     3721 tcgatccggc tccggtccgc cggcgcgtac cagccatcgg ggccgtacca ctgcgcgact
     3781 tcctcggggt cggtccaggc gcgatacacc tccgatggcg aagcgtcgaa atgccggctg
     3841 agcgtgacgg tgcggcggct ggcggcggcg gcggtcgtgt cggtcatggt cgtcggtccc
     3901 tctgcttgtg ctggagatgc gtgtcgagcc ggtcgatccg gcgctcccac gcccgggttc
     3961 gagcctcgag ccagccgcgc gcctcgagca gcggcgctgg ggcgaggcgg ctgggccgcc
     4021 attgcgcttc ccgttcccgc acgatgaggc cggcgcgttc gaggaccttc aggtggcgcg
     4081 agatggcggg cagactcacc gggaacggcg aagcgagctc gttgaccgtc gcggagccct
     4141 cggccagacg cgcaaggatc gagcgacgga tgggatcggc cagggcggcg aaggtctgat
     4201 cgagctggtg gagctggtcg gctggcattg aacctcccgg ttaattaacc gggcgattaa
     4261 acgcgagatt ggacggtcgt gtcaagaggg ctgggggacc ggcaggtaga atcgccgcgt
     4321 cagcgaggcg gccggatggc tgcgcgtcga gcggggcaac ccgagcggcg tgaggaaagt
     4381 ccgagctccc cagcgcaggg tagtgggtaa cccccatccg ccgcgaggcg aggaccagcg
     4441 ccacagtgac gatgccgtcg acctcggtcg tcggagtgaa acgcggcaag ctctacccgg
     4501 agcaaggcca aataggaggg cgaccacccg gcttgccgga gtacgtgcgc tgcacagccc
     4561 tcgggtcggc cgcatgagcc gtcgggcaac cggcggcccc agatggatgg ccatcgtcgc
     4621 catcgcttcg gcggaggcgg cacagaactc ggcttacagg ctgcctcgct ggcgccttct
     4681 caccgagacc cggcttggta cgctgcggac cctacggttc gcgtgaggag tgacggcgaa
     4741 tgctgaacga agtctctttc cggctcatgc accgtcacgg cgacgactac gagccgatgg
     4801 tcgagcacac gtccgccgat cacgaccctg aaaagggctg gttgcggggc gcgcgcatct
     4861 tccggtgcag gtcctgcgag gaagaggtcg tcctcgtccc gccgaccaac gagcccggcg
     4921 acacgtccgc ctgagcggac ccttcgcggg tcagtcctcg tcgggtcgga agatgtgggc
     4981 cgcggtgcag gcgttgcaga tcggcgtcgc tgcatcgagg tcgccgagcg ggatcgccag
     5041 cttgcgatcc gggtacaggc actcgacgtc gtagcgccgt tcgcccctgg gatccacgcg
     5101 gatcaggcgt cggaacgtgc accgcttgat ccgatgcggc gcaaggtgcg acggatcggg
     5161 cagcatcacg gactcgatga cgtgcggcag ggccatgggc ggatcgtggg cctcgagcga
     5221 gcgaaactcc ctcatcggcc cggcgccgac gagaatccgg gggagcctag caccgggtca
     5281 tgggccgcaa ccacggtgaa aaggtactag agtccgcggc caaccaaccg agaggagggg
     5341 agtggacggg tcgattcgcg agcctgcgca acgcacgctc acgccggccg tcctcgcctc
     5401 cgcggccttc gtcgcgatct gcgccgcgtt cgccatcacc tacgtggcgg cacgcggcgg
     5461 cctccagatg ccggtggcgc cgatcggctc ggccgcggcc gtcgcgagcc ccgcgccgac
     5521 caccgaccag ccgacgcagc ccgcgccgag catcgcaccg gtgccgacgc cactcccgtc
     5581 accgagtcag cccccgccct cggccacgcc agagcccacg ccggaaccca cggtcgagcc
     5641 cacgccgggg ccgaccctcg tcataccgac actccggccc aacgaccccc tcgccgacct
     5701 ccgtgcatgc ccgggccacc cgggctgcta ccggtatgtc gtccagcgcc tggacaccct
     5761 gtcgacgatc gccgatcgct tcctcgtgcc gatgggcacg atcctggcgc tcaacccgca
     5821 gatcacggac cccagcgtcg tcgtcacgaa cagcacgatg tacctgggtc gctcccggtt
     5881 cgttcgcctc gacccgtgcc cggacggagc gctctgctgg ttgtatgtcg tccgcgccgg
     5941 cgactcgctc gcggggatcg ccaaccggta cgccctcacc agggacgcaa tcctcgagat
     6001 caacccgggc ctgccgcggc cgctgaccat cgaccaggtc atccgcctgc cggacgtgcc
     6061 cgcctagaac gtcgcgccgc gcggcgccac cccgcgcggc accggcccgc gcgaaccgcg
     6121 actccacttg aaattgggga ttgacgaagc cctctcgcgc cctagaatgc ccagcaagtg
     6181 gtgagaagtg gggaaaagtg gggatccacc gcgagtccct tgggttccca gccctctcca
     6241 ccaccagcgg gccccggacg ggcactccgg atcccgcgac accgatgacg agcccgcggt
     6301 ccccgcgggc tcgtcgaatc tcggcaaatg ggcaatgggg tgcggtgggt gttcaccggc
     6361 gagtaccgac acacggtgga cgacaaggga cgactcgcga tcccggcgcg ctttcgggcg
     6421 cagctcgagg gcggcgtggt cctgtctcgc tggatcgacg gctgcctcgc catccatacg
     6481 cgagccggct gggacgccct ctccgccaag gttgccggcg tcgccctcac tgatcccgcc
     6541 ggccggctct tcgagcgcgc gatcttcgcc ggcgccctcg agctcgagat ggacaagcag
     6601 ggtcgcgtcc tcgtcgcgtc ctacctgcgc gatgaggtgg gtatcactgc cgacgcgctg
     6661 gtcatcggcg tccgcgacca cggcgagatc tgggcgcccg accgctggca gtcataccgc
     6721 cgcgcgatgg acgacccggc caagttcgcc gaagccatcc gcggactcgg gatctagcag
     6781 cgatgaccga cgaactgcca accgtgcacg tcccggtgat ggccgacgag gtcgtcgaga
     6841 tgctcgcgcc cgtggcgggc agccgccaca tcgacgcgac cctcggcggc ggcggccacg
     6901 ccgaacgaat cctcgaagct tccgaccctg atggccgcct cctcggcctg gatgccgatg
     6961 gggcggccat cgccagagtg cgagcccggc tcgcgccccg gttcgggcct cgcctggacc
     7021 tccgccaggc gaacttccgc gagctggccg
//
LOCUS       DATBSW010000355        12365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_245774, whole genome shotgun sequence.
ACCESSION   DATBSW010000355 DATBSW010000000
VERSION     DATBSW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245774"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VIF63_09745"
     rRNA            <1..401
                     /locus_tag="VIF63_09745"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            558..674
                     /gene="rrf"
                     /locus_tag="VIF63_09750"
     rRNA            558..674
                     /gene="rrf"
                     /locus_tag="VIF63_09750"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            716..1558
                     /locus_tag="VIF63_09755"
     CDS             716..1558
                     /locus_tag="VIF63_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8239706.1"
                     /translation="MTMADSEHERGKERTAAATPATVPGGSVTSSEDVGRDEATPRGC
                     PFLVADGGGWRLDLPTREHRCAAVSPPAPLSPEKQSRLCLTPAHTACATYLASIAARQ
                     VRLGSVPADRTTRWGLARTTTVIEDPGGWSVRILALLLDRRRWPAIPAVLLVTTLFTL
                     ALSGLRAGGSNPASATAGASGTQAAPALTSTPSTAPTSTFDSVTPTEAPPTEAPSAAA
                     PSPTAKSTPVPDATFRTYKVKEGDSLSAIAAKYNTTSRDIARLNNISVATPLHIGQVL
                     KIPN"
     gene            complement(1577..2452)
                     /locus_tag="VIF63_09760"
     CDS             complement(1577..2452)
                     /locus_tag="VIF63_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY8239707.1"
                     /translation="MPELPDLAIVADALHAALAGRPVVRAEASAPLAVRGTPAELSAL
                     VGQTLRSVRRRGKFITFELERDRIAVNAMLTGRFQLAAPKDKLPGQTAVILGFGARAK
                     PASKPARWTKGAAWLPADADPAEVRYRDPSQMGKVYVLPAGVARPVAAFEDAEQGPDA
                     DAPELTLEAWRERIRRHPGELKSLLRNQAFVAGIGNAYSDEILHAAKLSPFRRRSSLA
                     PEEVDALYVATRTTLARAIDLLEERVPPTFEKQVRDFLAVHLSGGEPCPRCGTRLSEV
                     SSRSEATTWCRGCQR"
     gene            2664..4967
                     /gene="rpsA"
                     /locus_tag="VIF63_09765"
     CDS             2664..4967
                     /gene="rpsA"
                     /locus_tag="VIF63_09765"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY8239708.1"
                     /translation="MTDEQQGGTKPPAEASAEAPTSEPSVGAVAVADEPATSPSLDTE
                     AMGSEAAAADAAPTTEPAPAAEAAAAPVDEMTDVEPEPAAAVSEAAAPEAPAAEAPAE
                     VVADADAPIADVAATVLPDAAVEALADAAASAAEATPAATEATAEAEADDAPAADDAA
                     EDDAAAAAEAAVEAPAPKPAPVVPTGPEPTTMEELLAEQDTDIKSFKHGDVVEGTVVR
                     IDKDEVLVDIGAKSEGVVSNRELFGRNTEAQPALNIGDTVLVYVLQPESPEGHVVLSL
                     RRAGLERKWRSMQEQFEAGVIIEAPVIDHNKGGLIVDCGIRGFVPISQIVDFPRRPQN
                     DQPRDAAQEIAEKLQPFVGRKLRLKILEVNRKANRLILSEKVALYEERREKRDELFSS
                     LQVGQKVTGHVRSIAPFGVFVDLGGIDGLVHKSELSWNKVNNPEGGYRVGEEVEAEVI
                     DINHERGRISLSIRRLQPDPWHSTVADFKVGDVIDGTVTKLVNFGAFVRVRDGLEGLI
                     HISELSNQRVAHPGDVVHEGQNLKLRIISLDSERHRLGLSLKQAEDQPARPIEQDAPA
                     AEAGAMPMPRPERTERPERGDRRPRNDRDRPRPYSASDAVQEPEGGIDTTLAAAFAGV
                     REQLARAEAGDEGDTEVTADAEAEAPAAEAAAEAPAAEAVVETAAVEDEAPEPATAAE
                     AEAVAEPEAAAEPEAAAEPEVPVEAVAEPAAEVAAPEPEPAKGDEPAAEADADTAGEA
                     TADAEPAKGDEPATAAEAASDEDAKAT"
     gene            5171..7645
                     /locus_tag="VIF63_09770"
     CDS             5171..7645
                     /locus_tag="VIF63_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY8239709.1"
                     /translation="MERFDKFTDRARKVLTLAQDEAQRFNHNYIGTEHLLLGLVREGE
                     GVAARVLENMNVELAKVRTAVEFIIGRGDRPVVGEVGLTPRAKRVIELAIDEARRLGH
                     NYIGTEHLLLGLVREGEGIAAGVLESLGVNLDKVRHEVIRVLSQSSSVGPAQETKRSS
                     KTPTVDALGINLTEAARAGKLDPVIGREKEIERVIQILGRKTKNNPALIGEPGVGKTA
                     IAEGLAHRIVAGDVPDILLNKRVLTLDIGSLVAGTKYRGEFEERLKKIIEELRNTNDA
                     VLFIDELHTLVGAGAAEGAIDAANILKPPLARGELQCIGATTLDEYRKYIERDAALER
                     RFQPVMVEEPSLEQTIEILMGIRERYEQHHKVTITDDAVKAAADLSIRYITDRHLPDK
                     AIDLIDEAASRVRLRHSSAPPPLREAQRELDKLTKEKDGAINSQDYESAAKLREDEAT
                     LREKVDGLRADWQATIAGDAPQVDEEEIAQVVAMWTGIPVTRISEAESERLLHMEDAL
                     HQRVVGQQEAIDIVAKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELAKALAEFMFG
                     SEDTLIKIDMSEFMERHNVSRLVGAPPGYVGFDEGGQLTEAVRRKSYCVVLLDEVEKA
                     HPEVFNILLQILEDGQLTDAKGRRVDFRNTIIIMTSNLGARQLQTNSSLGFRPVAGDE
                     PGRAAQSYDLMKEKVQAELKTSFRPEFLNRIDATVVFRSLTVDEIREIVDFMLRRVRD
                     QLKAQNMVLEVTQAAKDHLIKLGWDPAFGARPLRRAIQNSIEDVLAEHLLLGRYPSGS
                     TIVVDANGDDGLVIQAAEQKTPVEVS"
     gene            7654..9141
                     /gene="radA"
                     /locus_tag="VIF63_09775"
     CDS             7654..9141
                     /gene="radA"
                     /locus_tag="VIF63_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY8239710.1"
                     /translation="MARAQSRFVCQACGADYPRWEGQCRSCAAWNTLVETVVTQRTRT
                     AAARSARASHGAPEPVALSAPADGPTERLRTGLGELDRVLGGGIVPGSLLLIGGEPGV
                     GKSTLLLQVAGGVAGGVGGDATGGSGVLYATGEESAGQVRMRAARLGLGSGPAADAVM
                     VLATSEVGAIIEAARARRPALVVVDSIQTMTVDDLDGPAGSVGQVRESALRLMELAKG
                     EGIAVVLVGHVTKDGSIAGPRTLEHLVDAVIDLGGERGGVMRLVRAAKNRFGSTDELG
                     VLEMGESGLREVADPGRAFLADHEGAAPGSVVAATLEGSRPLLIEVQALVSPAGYSTP
                     ARRASGIDPTRLALLLAVLGRRAGIGLGSHDVYANLAGGVSISEPGLDLAVALALASS
                     LRDRPIDTRTVAVGEVGLLGELRPVAGIDRRLREAARLGFERAIVPGARAAATSIPGI
                     VTVPAANLREALEAALGVGDNRDNERRQRRPVTAVSISPERPVVG"
     gene            9227..10276
                     /locus_tag="VIF63_09780"
     CDS             9227..10276
                     /locus_tag="VIF63_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY8239711.1"
                     /translation="MIRTIHFLGAALGGLIGITLVIAQLAVFKPVEGSAVLVVAWVIA
                     WIVLGFTLLPYLTIIPAMRALGAVQQLSTAEFVTAIIGLFLGLLLGLLLGLPLSALPP
                     PIGTIAPLGISLFLGLAMVGLTVAKRHDLITAAEGLGLIRRAAADGEGTIPRILVDTS
                     AIIDGRIADIADSGFLYGILEIPRFVLDEVQRLADSSDSMRRTRGRRGLEILARMRKS
                     GTTPVVVIDEDVPGTPEVDSKLVALAQRHGRAVLTNDLNLNRIADLQGVRVLNVNSLA
                     NAVKPALLPGEDLRVRVIQPGKDAGQGVGYLDDGTMVVVEGGAKAIDSELDVTVTRVL
                     QTVAGRMVFAHPRQA"
     gene            10281..11477
                     /gene="ispF"
                     /locus_tag="VIF63_09785"
     CDS             10281..11477
                     /gene="ispF"
                     /locus_tag="VIF63_09785"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY8239712.1"
                     /translation="MSGVVADAVVVAAGSSERMGGRDKLAADLYGKPVLAWSLAALAA
                     SPLVDRIALVQTAEQAAGPRPSWLPSKVIAVVAGGNRRQESVAAGIRALEAAPPDDGP
                     DGGSRVLLVHDGARPLVTRALVEAVARAAAEHGAAIPVVPVTETLKRVDREVVTATID
                     RAGAAVAQTPQGVRWDVLREALRRVRPEDALELTDEAALLEAADIAVRAVPGDPANLK
                     ITRPDDLAMAESRLAAAGATRTGFAADGHAFGPGTGLRLGGVEIAGAPRLHGHSDGDA
                     ALHAICGALLGSIALGDLGGLHPADTRTPRGIASADLLVSVREALAAEGWRPATVDVS
                     IRAARPLLADHLPRMREAIAGLLELELGAVSVKASTGNLSGDPGAGRTIEAEAIATVV
                     RRTSDR"
     gene            11485..>12365
                     /locus_tag="VIF63_09790"
     CDS             11485..>12365
                     /locus_tag="VIF63_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY8239713.1"
                     /translation="MSLRLHDTLTGDVRPLEPLEAGHVRIYSCGPTVYGPTHIGNFRS
                     FLFADVLVRYLRYRGLRVTWVMNVTDIDDRIIERANAAGETTAELTDRWLARFKADFE
                     ALRMTPPDVLPRATDHIDAMAELIAQLLETGNAYRTDDGSIFFRISSWPAYGKLARID
                     ASQLRVGERVEADDYGKDDVRDFAVWKGPKPGDPSWETAVGPGRPGWHIECSAMSMRH
                     LGPSFDIHTGGVDLIFPHHEDEIAQSEAVTGQTFVQTWLHCDHLRLGGTKMARRVGNI
                     ARVADLLAEGASPRALR"
BASE COUNT         1975 a         4442 c         4296 g         1652 t
ORIGIN      
        1 ctcgatgtcg gctcgtcgca tcctggggcg gaagtacgtc ccaagggttt ggctgttcgc
       61 caattaaagc ggcacgcgag ctgggttcag aacgtcgtga gacagttcgg tctctatccg
      121 ccgtgggcgt agggaacttg acaggagctg ctcctagtac gagaggaccg gagtggacga
      181 accgctggtg tgccagttgt cccgccaggg gcaccgctgg gtagctatgt tcggcaggga
      241 taagcgctga aagcatctaa gtgcgaagct cgcctggaga tgaggttccc caccgggtta
      301 accgggtaag accgcaggta gaccacctgc ttgataggcc gcatgtggaa gtccggtgac
      361 ggatgaagct gagcggtact aatggtcgag ggcttgacca cttcagcttc tgacccaggg
      421 tcaggggcat gaagggaaca atcgggatat cggatcccac cacaacatga cgaagtcaaa
      481 gatctcgaga cttctcggcg cccgcgttcg actcgtcgag cgcacggacg cagccgatca
      541 caatccagca gcaagatgct ggtgacgcta gcggagaggc catacccgtt cccatcccga
      601 acacggaagt caagctctcc agcgccgaag atactgagag ggcagccttt cgggaaaata
      661 ggtcgtcgcc agcatcttgt gctttccggc ggcttccttg gctatgctgg ctcggatgac
      721 gatggccgat tccgagcacg agcgcggcaa ggaacgcacc gccgccgcca ccccggccac
      781 cgtcccgggc ggcagcgtga cctcctcgga ggacgtcggc cgggacgaag cgacaccccg
      841 cggctgcccg ttcctcgtcg ccgatggcgg tggctggcgg ctcgacctgc cgacccgcga
      901 gcaccgctgt gcggccgtga gcccgccggc gccactctcg cccgagaagc agtcccggtt
      961 gtgcctcacg ccggcgcaca ccgcctgcgc gacgtacctc gcgtccatcg cggcccgaca
     1021 ggttcgcctc ggctcggtgc ccgccgatcg cacgacccgc tggggcctcg cccgcaccac
     1081 gaccgtcatc gaggatccgg gcggctggag cgtgcgaatc ctcgccctgc tgctcgaccg
     1141 tcgacgctgg ccggcgatcc ccgccgtcct cctcgtcacg accctgttca cgctcgcgct
     1201 gtcgggcctt cgggccggcg gttccaatcc cgcctcggcg acggccggtg cgagcgggac
     1261 gcaggctgcg cctgcgctca cctcgacgcc cagcacggcg ccaacatcga cgttcgacag
     1321 cgtgaccccg accgaggcgc ccccgaccga ggcaccgtcc gctgctgcgc cgagccccac
     1381 cgcgaagagc acgccggtgc ccgacgcgac attccgcacc tacaaggtga aggagggcga
     1441 cagcctcagt gcgatcgcgg ccaagtacaa caccacgtcg cgcgacatcg cccggctcaa
     1501 caacatctcg gtggcgaccc cgctccacat cgggcaggtc ctgaagatcc cgaactagcg
     1561 ccggcggaat cccgagctag cgctggcagc cccggcacca ggtcgtcgcc tcgctgcgcg
     1621 agctgacctc gctcaagcgg gtaccgcagc ggggacaggg ctcgccgccg ctaaggtgca
     1681 cggcgaggaa gtcgcggacc tgcttctcga acgtgggcgg gacgcgttcc tccaagaggt
     1741 cgatcgcgcg cgcgagggtc gtccgcgtcg ccacgtacag cgcgtccacc tcttcgggcg
     1801 cgagcgatga cctgcgccgg aacgggctga gcttcgccgc atgcaggatc tcgtcgctgt
     1861 acgcgttgcc gatcccggcc acgaacgcct ggttgcgcag gagcgacttg agctcgccgg
     1921 gatggcgtcg gatccgctcc cgccaggcct cgagcgtgag ctcgggcgcg tcagcgtccg
     1981 gtccctgttc ggcgtcctcg aacgcggcaa cgggccgcgc gacgcccgcc gggagcacgt
     2041 agaccttgcc catctggctt gggtcgcggt agcggacctc ggccgggtcg gcgtcggcgg
     2101 ggagccaggc tgcgcccttc gtccatctcg cgggcttgga cgccggcttc gcgcgggcgc
     2161 cgaagcccag gatgaccgcg gtctggccgg ggagcttgtc cttcggcgcg gcgagctgga
     2221 accggccggt gagcatcgcg ttcacggcaa tacgatcacg ctcgagctcg aaggtgatga
     2281 acttgccgcg ccggcgcacg gagcgcaggg tctggccgac gagggcggaa agctcggccg
     2341 gcgtgccgcg cacggcaagg ggagcggacg cctcggcgcg tacgactggc cggccggcga
     2401 gcgcagcgtg gagagcgtcc gcgacgatcg ccagatcggg cagctcgggc acggccgcgc
     2461 gattgtagcc gcgtggacgg gcatgctaga ctccgcgggc ccttcggggc ggaacagctg
     2521 aatgcctcgc tcaccccgat cacgacctcg aaggagcgga ccgcggccca acgatccacg
     2581 gaccacttgc tcgcaggaac gacggcctga gcgatgcgct cgggccgttc cgattcgagg
     2641 gagatgatcc acaggagaca gaattgaccg acgaacaaca gggcgggacg aagccgccgg
     2701 ccgaggcatc cgccgaagcc ccgacttcag agccttccgt tggcgccgta gccgtcgccg
     2761 acgaacccgc caccagcccg agcctcgaca ccgaggcgat gggcagtgaa gccgccgcag
     2821 cggatgcggc ccccaccacc gagccggctc ccgccgctga agcggccgcc gcgcccgtgg
     2881 acgagatgac cgatgtcgag cccgagccgg ccgcggccgt ctccgaggcc gccgcgcccg
     2941 aggcgcccgc cgccgaagcg cctgccgaag tcgtcgccga cgctgacgcg ccaattgccg
     3001 acgttgcggc aacagtgctt cccgacgctg cagtcgaggc gttggccgac gcagccgcgt
     3061 ccgccgccga ggcgacgccc gccgccacgg aggcgaccgc cgaggccgag gccgatgacg
     3121 cgcccgcagc cgatgacgct gccgaggacg acgctgcggc tgccgccgaa gcggccgtcg
     3181 aggcccctgc gcccaagccc gctcccgtcg tgcccaccgg ccccgagccg acgacgatgg
     3241 aggagctgct cgccgagcag gacaccgaca tcaagagctt caagcacggc gatgtcgtcg
     3301 agggcaccgt cgtccgcatc gacaaggacg aggtcctggt cgacatcggc gccaagagcg
     3361 aaggcgtcgt cagcaaccgc gagctgttcg gtcgcaacac cgaggcccag ccggcgctga
     3421 acatcggcga cacggtcctc gtgtacgtcc tccagcccga gtcgccggaa ggccacgtcg
     3481 tcctctcgct gcgacgcgcc ggcctcgagc gcaagtggcg ctcgatgcag gagcagttcg
     3541 aggcgggggt catcatcgag gccccggtca tcgaccacaa caagggcggt ctcatcgtcg
     3601 actgcggcat tcgcggcttc gtgccgatca gccagatcgt cgacttcccg cgccggcccc
     3661 agaacgacca gccgcgcgac gcggcccagg agatcgccga gaagctccag ccgttcgtcg
     3721 gccgcaagct gcggctcaag atcctcgagg tcaaccgcaa ggcgaaccgc ctgatcctgt
     3781 ccgagaaggt ggccctgtac gaggagcggc gcgagaagcg cgacgagctc ttcagcagcc
     3841 tccaggtcgg ccagaaggtc accggccacg tccgctcgat cgccccgttc ggcgtcttcg
     3901 tcgacctcgg cggcatcgac ggcctcgtcc acaagagcga gctctcgtgg aacaaggtca
     3961 acaaccccga gggcggctac cgcgtcggcg aagaggtcga ggccgaagtc atcgacatca
     4021 accacgagcg cggccggatc agcctgtcga tccgacggct gcagcccgat ccgtggcact
     4081 cgaccgtcgc cgacttcaag gtcggggacg tcatcgacgg cacggtcacg aagctcgtca
     4141 acttcggcgc gttcgtgcgc gtgcgcgacg gcctcgaggg cctgatccac atcagcgagc
     4201 tctcgaatca gcgcgtcgcc catcctggcg acgtcgtcca cgagggccag aacctcaagc
     4261 ttcggatcat cagcctcgac tccgagcgcc atcgcctcgg cctgagcctc aagcaggccg
     4321 aggaccagcc ggcgcgaccg atcgagcagg acgccccggc tgccgaggcc ggcgccatgc
     4381 cgatgcctcg tccggagcgc accgagcgac ccgagcgcgg cgatcgacgc ccgcgcaacg
     4441 atcgcgatcg accccggccc tacagcgcat ccgacgcggt gcaggagccg gaaggcggca
     4501 tcgacacgac cctggccgcg gcctttgccg gtgtgcgcga gcagctcgcg cgtgccgagg
     4561 ccggcgacga gggtgacacc gaggtgacag ccgacgccga ggcggaggcc cccgcggccg
     4621 aagccgcggc cgaagcgccc gcagccgagg cagtggtcga gacggccgcc gtcgaggacg
     4681 aggcgcccga gcccgcgaca gcggctgagg cagaggccgt cgcagagccc gaagccgccg
     4741 cagagccaga agccgcagcg gagcccgagg tccctgtcga ggctgtcgcc gaacccgccg
     4801 ccgaggtcgc cgcgcccgag cccgagcccg ccaagggcga cgagcccgcc gcggaagctg
     4861 acgcggatac cgctggcgag gcaaccgccg atgccgagcc ggcgaagggc gacgagcccg
     4921 ccaccgcggc cgaggcagcc tccgacgagg acgccaaggc cacctagccc cgcgacggca
     4981 cggcgcgacg ccgatcgcgc cgggcaaggc aaccgcctga ccctgaccag cgtctccggc
     5041 gcctgatccg gtcaccgatt cgcagcgaat ccttcaccga aggggcccgc tgcacggtca
     5101 aaacgggccg aatcgcatgc taggatgacc acgcgccagg aaggcgcagc aggggaacgg
     5161 gcgttcgact ttggagcgat tcgacaagtt cacagaccgg gcacggaagg tgctgacgct
     5221 cgcccaggac gaggcgcagc gcttcaacca caactacatc gggaccgagc acctcttgct
     5281 cggcctggtg cgggagggcg agggcgtcgc cgcgcgcgtc ctcgagaaca tgaacgtcga
     5341 gctggcgaag gtccggacgg cggtcgagtt catcatcggg cgtggcgatc gaccggtcgt
     5401 cggtgaggtc gggctgaccc cgcgcgcgaa gcgcgtcatc gagctggcca tcgacgaggc
     5461 gcgccggctc ggccacaact acatcggcac ggagcacctg ctcctcggcc tcgttcgcga
     5521 gggcgagggc atcgctgccg gcgtgctcga gagcctcggc gtcaacctcg acaaggtccg
     5581 ccacgaagtc atccgcgtcc tgtcgcagtc gtcgtcggtc gggcccgccc aggagacgaa
     5641 gcgcagcagc aagaccccga cggtcgatgc cctcggcatc aacctcaccg aggccgcgcg
     5701 cgcgggcaag ctcgatccgg tcatcggccg cgagaaggag atcgagcggg tcatccagat
     5761 ccttggccgg aagacgaaga acaacccggc cctgatcggc gagcccggcg tcggcaagac
     5821 cgcgatcgcc gagggcctcg cgcaccggat cgtcgcaggc gacgtgcccg acatcctgct
     5881 caacaagcgc gtcctcaccc tcgacatcgg ctcgctggtc gcgggcacca agtaccgcgg
     5941 cgagttcgag gagcgcctca agaagatcat cgaggagctc cggaacacca acgacgcggt
     6001 gctcttcatc gacgagctgc acacgctcgt cggcgcgggc gcggccgagg gcgcgatcga
     6061 cgccgcgaac atcctcaagc cgccactcgc ccgcggcgag ctccagtgca tcggcgcgac
     6121 cacgctggac gagtaccgca agtacatcga gcgtgacgcg gccctcgagc gccgcttcca
     6181 gccggtcatg gtcgaggagc ccagcctcga gcagaccatc gagatcctca tgggcatccg
     6241 cgagcgctac gagcagcacc acaaggtcac gatcaccgac gacgcggtca aggccgccgc
     6301 ggacctgtcg atccggtaca tcacggaccg ccacctgccc gacaaggcga tcgacctcat
     6361 cgacgaggcc gcgagccgcg tccgtctccg ccactcctcg gcgccgccgc ccctgcgcga
     6421 ggcgcagcgc gagctcgaca agctgaccaa ggagaaggac ggcgcgatca actcccagga
     6481 ctacgaatcc gcggcgaagc tgcgcgagga cgaggcgacg ctgcgcgaga aggtcgacgg
     6541 gctgcgcgcc gactggcagg cgacgatcgc cggcgacgcg ccgcaggtcg acgaggagga
     6601 gatcgcccag gtcgtcgcga tgtggaccgg catcccggtc acgcgcatct ccgaggccga
     6661 gtccgagcgg ctgctgcaca tggaggacgc gctccaccag cgcgtcgtcg gccagcagga
     6721 ggcgatcgac atcgtcgcca aggccgttcg ccgagcccgg gccggcctca aggacccgaa
     6781 gcgcccgatc ggatcgttca tcttcctcgg cccgaccggc gtcgggaaga ccgagctcgc
     6841 caaggccctc gccgagttca tgttcggcag cgaggacacg ctcatcaaga tcgacatgag
     6901 cgagttcatg gagcggcaca acgtgagccg gctcgtcggg gcgcctccgg gctacgtcgg
     6961 gttcgacgaa ggcggccagc tgaccgaggc cgtgcgccgc aagagctact gcgtggtgct
     7021 cctcgacgag gtcgagaagg cccaccccga ggtgttcaac atcctgctcc agatcctcga
     7081 ggacgggcag ctcacggacg ccaagggtcg ccgcgtcgac ttccggaaca cgatcatcat
     7141 catgacctcg aacctcggtg cgaggcagct ccagacgaac tcctcgctcg gcttccggcc
     7201 ggtcgccggc gacgagcccg gccgcgccgc gcagtcgtac gacctcatga aggagaaggt
     7261 ccaggcagag ctcaagacga gcttccggcc cgagttcctg aaccgcatcg atgcgaccgt
     7321 cgtcttccgc agcctcaccg tcgacgagat ccgcgagatc gtcgacttca tgcttcggcg
     7381 ggtgcgcgac cagctcaagg cccagaacat ggtcctcgag gtgacccagg cggccaagga
     7441 tcacctgatc aagctgggct gggatcccgc cttcggggcg cggcccctgc gccgcgccat
     7501 ccagaactcc atcgaggacg tgctcgcaga gcacctcctc ctcggccgct acccgtcggg
     7561 ctccaccatc gtggtggacg cgaacggcga cgacggcctc gtgatccagg ccgcggagca
     7621 gaagaccccc gtcgaagtct cgtaggcgcg gagatggctc gcgcccagag ccggttcgtc
     7681 tgccaggcgt gcggcgccga ctacccacga tgggaaggcc agtgccgctc gtgtgccgca
     7741 tggaacacgc tcgtcgaaac ggtggtcacc cagcgcacgc ggaccgcggc tgcgcgctcg
     7801 gcgcgcgcca gccacggggc gcctgaaccg gtcgcgctct cggcgcccgc cgacggccca
     7861 accgaacggt tgcgcaccgg gttgggcgag ctcgatcgcg tgctgggcgg cgggatcgtg
     7921 cccgggtcgc ttctgctgat cggcggcgaa ccgggcgtcg gcaagtcgac gttgctcctc
     7981 caggtcgcgg gcggggtggc cggcggcgtg ggcggtgacg ccacgggagg gagcggcgtc
     8041 ctctacgcga ccggcgaaga atcggccggg caggtgcgca tgcgcgcggc gcggctgggg
     8101 ctggggtcag gtcccgcggc cgatgcggtg atggtcctcg ccacgagcga agtcggcgcg
     8161 atcatcgagg ctgcgcgggc gcggaggccg gccctcgtcg tggtcgattc gatccagacc
     8221 atgaccgtcg acgacctcga cgggcccgcc ggctcggtcg gccaggtgcg cgaatctgcg
     8281 ctcaggctga tggagctggc aaagggcgag ggcatcgcgg tcgtgctcgt cgggcacgtg
     8341 acgaaggacg gctcgatcgc cggcccgcgc accctcgagc acctcgtcga cgccgtgatc
     8401 gacctcggcg gggagcgcgg cggcgtgatg cgcctcgtgc gtgccgcgaa gaaccggttc
     8461 ggctccaccg acgagctggg cgtcctcgag atgggcgagt ccgggctccg cgaagtggcc
     8521 gatccgggtc gcgcgttcct ggccgaccac gagggcgccg cgcccgggag cgtcgtcgcg
     8581 gccacgctcg agggcagccg gccgttgctc atcgaggtcc aggcgctggt ctcaccggcg
     8641 gggtactcga cgccggcgcg acgcgcgtcg ggcatcgacc cgacacggct cgccctgctc
     8701 ctcgccgtgc tcggccgccg agccgggatc ggcctgggca gccatgacgt ctacgcgaac
     8761 ctggcgggcg gcgtgagcat cagcgagccc ggcctcgatc tcgccgtcgc gcttgccctc
     8821 gcctcgtcat tgcgcgaccg cccgatcgac acgcgcacgg tcgcggttgg cgaggtcggg
     8881 ctgctcggcg agttgcggcc ggtcgccggc atcgatcggc gcctgcgcga ggccgcgcga
     8941 ctcggcttcg agcgcgcgat cgtgcccggt gcacgcgcgg cggccacatc gatcccgggg
     9001 atcgtgacgg tgccggccgc aaacctgcgc gaggcgctcg aggcggccct cggcgtcggc
     9061 gacaaccgcg acaacgagcg caggcagcgc cggccggtga cggcagtatc gatctcgccc
     9121 gaacggccgg tggtagggta ggcttccaag cccgacgatc gagccgcgac ggttacgacc
     9181 ggcgccgggc cgttccgacg cgatcgcggt cattcgaggc gtcctgttga tccgaaccat
     9241 tcatttcctg ggggcagcgc tcggcgggct catcgggatc acgctcgtca tcgcacagct
     9301 cgcggtcttc aagcccgtcg agggttcggc cgtgctcgtc gtggcgtggg tgatcgcctg
     9361 gatcgtgctc ggcttcacgc tcctgccgta cctcacgatc atccccgcga tgcgggccct
     9421 gggcgccgtc cagcagctgt cgaccgcgga gttcgtgacc gccatcatcg ggctcttcct
     9481 cggcctgctc ctcggcctgc tgctcgggct gcccctgtcc gcgctgccgc ccccgatcgg
     9541 gacgatcgcc ccgctcggca tatcgctctt ccttgggctt gcaatggtcg gtctcacggt
     9601 cgcgaaacgc cacgacctga tcacggcggc cgaggggctg ggcctgatcc ggcgcgccgc
     9661 ggccgatggt gagggcacga tcccgcgaat cctcgtcgac accagcgcga tcatcgacgg
     9721 gcgcatcgcc gatatcgccg actcgggctt cctgtacggg atcctcgaga tcccgcgctt
     9781 cgtgctcgac gaggtccagc gcctcgccga cagctcggac tccatgcgcc gcacccgcgg
     9841 tcgccgcggg ctcgagatcc tggctcgcat gcgcaagagc ggcacgacgc cggtcgtcgt
     9901 catcgacgag gacgtacccg gcacgccgga ggtcgactcg aagctggtcg cgctcgcgca
     9961 gcgccacggt cgtgcggtcc tcaccaacga cctgaacctc aaccgcatcg ccgacctgca
    10021 gggcgtgcgc gtcctcaacg tgaactcgct cgccaatgcc gtcaagccgg cgctgctgcc
    10081 gggcgaggac cttcgcgtgc gcgtgatcca gcccggcaag gacgccgggc agggtgtcgg
    10141 ctatctcgat gacggcacga tggtcgtcgt ggaaggtggc gcgaaggcga tcgacagcga
    10201 gctcgatgtc accgtcaccc gtgtcctcca gaccgtggcc ggccgcatgg tgttcgccca
    10261 tccgcgacag gcctgatcgg gtgagcgggg tcgtcgcgga cgcggtggtg gtcgccgcgg
    10321 gcagctcgga gcggatgggc ggccgcgaca agctcgccgc ggacctctat gggaaacccg
    10381 tcctcgcctg gtcgctggcg gcgctcgccg cttcgcccct cgtcgaccgg atcgcgctgg
    10441 tgcagaccgc cgaacaggcg gccggaccgc gccccagctg gctcccttcg aaggtcatcg
    10501 cggtggtcgc cggtggcaac cggcgccagg agtcggttgc cgcgggcatc cgcgcgctcg
    10561 aggcggcccc gccggacgac ggacctgacg gtggctcgcg cgtgctgctc gtgcacgatg
    10621 gcgctcgtcc cctcgtgacc cgggcgctcg tggaggcagt cgcgcgagcc gccgcggagc
    10681 acggcgcggc aatcccggtc gtgccggtca ccgagacgct caagcgcgtg gatcgcgagg
    10741 tcgtcacggc gacaatcgat cgggcgggcg ccgcggtggc acagacgccc cagggcgtgc
    10801 gttgggacgt cctgcgggag gccctccgac gcgtgcggcc ggaggacgcc ctcgagctca
    10861 ccgacgaggc cgctctgctc gaggccgcgg atatcgcggt tcgtgcggtg cccggcgatc
    10921 ccgcgaacct gaagatcacc cggcctgacg acctcgccat ggcggagtcg cgactcgccg
    10981 ccgccggcgc aacgcggacc gggttcgccg ccgacggcca cgcgttcggg ccgggcacgg
    11041 ggctccggct cggcggggtc gagatcgccg gtgcgccacg actccacggc cactccgacg
    11101 gcgatgctgc gctccacgcg atctgtggcg cgctgctcgg ctcgatcgcc ctcggcgatc
    11161 tcggcgggct gcatccggcc gacacgcgga cgccgcgcgg catcgcgagc gcggacctgc
    11221 tcgtgagcgt gcgcgaggcc ctcgcggccg agggctggcg gccggcgacg gtcgacgttt
    11281 ccatccgcgc cgcccggccg ttgctggccg accacctgcc ccggatgcgc gaggccatcg
    11341 ccgggctgct cgagctggag ctcggcgccg tcagcgtgaa ggcgtccacg ggcaacctct
    11401 cgggcgaccc gggcgcgggg cggacgatcg aggccgaggc gatcgccacc gtcgtgcgtc
    11461 gaacgagcga ccgatgagcg accgatgagc ctccgcctcc acgacacgct caccggcgac
    11521 gtgcggccgc tcgagccgct cgaggcaggg cacgtccgga tctactcgtg cggaccgacc
    11581 gtctacggac cgacccacat cggcaacttc cgctcgttcc tgttcgcgga cgtcctcgtg
    11641 cgctacctgc gctaccgcgg gctccgcgtc acctgggtca tgaacgtcac cgatatcgac
    11701 gaccgaatca tcgagcgggc aaacgccgcg ggagagacga ccgccgagct gaccgaccgc
    11761 tggctcgccc gcttcaaggc cgacttcgag gcgctgcgca tgacaccgcc cgacgtcctg
    11821 ccacgggcga ccgaccacat cgacgccatg gccgagctga tcgcccagct cctcgagacg
    11881 ggcaacgcct atcgcaccga cgacggctcc atcttcttcc ggatcagctc gtggcccgcc
    11941 tacggcaagc tggcgcggat cgacgcctcc cagctccggg tcggcgagcg cgtcgaggcc
    12001 gacgactacg gcaaggacga cgttcgcgac ttcgcggtct ggaaaggccc gaagccgggc
    12061 gatccgtcgt gggagacggc ggtcggaccg gggcgtccgg gctggcacat cgagtgctcg
    12121 gcgatgagca tgcggcacct cggtccgtcg ttcgacatcc acaccggcgg ggtcgacctg
    12181 atctttccgc accacgagga cgagattgcc cagagcgagg cggtgaccgg gcagacgttc
    12241 gtccagacgt ggctgcactg cgaccacctt cggctcggcg gcaccaagat ggcccgccgc
    12301 gttggcaaca tcgcgcgtgt cgcggacctc ctggccgagg gcgcgtcacc gcgggccctg
    12361 cgcta
//
LOCUS       DATBSW010000356         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_915291, whole genome shotgun sequence.
ACCESSION   DATBSW010000356 DATBSW010000000
VERSION     DATBSW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915291"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VIF63_09795"
     CDS             complement(<1..916)
                     /locus_tag="VIF63_09795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY8239714.1"
                     /translation="MTHPTTTDPGPAVAELDTIVSLAKRRGFVYPSSEIYGGINAVWD
                     YGPLGVELKNNVKRAWWRAMVQERNDIVGLDAGILMHPQVWVTSGHVGSFSDPLVECQ
                     TDHRRFRLDELPGGENLSASDLRDPGIVERLGLKCPVDGGPLSAPRQFNLMFKTHMGP
                     IEEDSNAVYLRPETAQGSYVNFKNVQGSSRKKLPFGIAQIGKSFRNEISPGNFVFRMR
                     EFEQMEMQYFVRPDEAARIFEEWLPRRWDWYVQYGVTPERLRFREHAPDELAHYAKKA
                     VDVEYRFPFGWKELEGIHNRGDFDLSRHQ"
     gene            1041..1619
                     /locus_tag="VIF63_09800"
     CDS             1041..1619
                     /locus_tag="VIF63_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239715.1"
                     /translation="MDPSPPPPQRRESLVSSEQSARELAGELGGAMNAVILEPLVADA
                     VEGIRFTLLAVVMVPIGIVVLILSFFVYFGAFLALGSPTGSLGAIVTGVWLIGTLVAL
                     VVVYRAVSRRMLGRGRLLGARPAPPKAAFAGGALSELLGGEPQAAVAAIAPAPSLAEL
                     DARLAPMSPPTSAPTVPPTAAGATPSQQDAPV"
     gene            complement(1616..2092)
                     /locus_tag="VIF63_09805"
     CDS             complement(1616..2092)
                     /locus_tag="VIF63_09805"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8239716.1"
                     /translation="MTVELRDIVTDEDRTAAVALRVAPDQEQFVATVQASLDEAVLYP
                     EACARYWAVYDGPQIVGFAMLSDGIAAEVLAVDPTLVGPYFLWRLLIDQRHQRRGYGS
                     ATIDALRAYLKDRPGAVELLTSCGQGEGSPCPFYERYGFVRTGQIHEGEVVLTLAL"
     gene            complement(2116..2589)
                     /locus_tag="VIF63_09810"
     CDS             complement(2116..2589)
                     /locus_tag="VIF63_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239717.1"
                     /translation="MSTKADLSATNWAALVAAAPAIARAVSASSGSGGQSEKELNAFV
                     QFASDAATGAGPDDILGQLVSDIGALLAVGVEPVSGDAYTDGLEQARRAGAIIAVELE
                     PAEAEAIRAWYLAAAQAVAAAVREGGVLGIGATDISNWERQTIQSIADALGAEAS"
     gene            complement(2586..3395)
                     /gene="recO"
                     /locus_tag="VIF63_09815"
     CDS             complement(2586..3395)
                     /gene="recO"
                     /locus_tag="VIF63_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8239718.1"
                     /translation="MPAPRRYVTEAIVLSRFDYGEADRILTLITPGGGKIKAIAKGIR
                     RQKSRIGGSLEPFAELRLALAQGRTFEVVTQVEVIHPWLRMRDDLVSFGTASYLAELA
                     NGTLEERHGAESVYLLLKRAYEILDAGMAPGRVARWFEMHLADELGVRPEVDRCVECG
                     RLIESDERYRWMPPLGGVLCERCPGPPYERAGLSLEALKLLKAYQRQDVEALAALRVR
                     PETERDVEIAMREFLAYSLDRRPRSLAFLDEVRAGAAERAAEREAVGTLRP"
     gene            3763..4782
                     /locus_tag="VIF63_09820"
     CDS             3763..4782
                     /locus_tag="VIF63_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8239719.1"
                     /translation="MALSTSPDIAELIADTEAEILRLVEGSDPSTAGLYEMCRYHLGL
                     DGSGSTGKRMRPLLGLLAYSSITGDHRRALPGAAAVELGHNFSLVHDDIEDDDRERRH
                     RPTLWALHGVPQAINTGDVLFSLSRIALHRLTDLGFPDKTVLRLMRLYDHTCVALCEG
                     QYLDIWMAGRDTDLSVELYFDMIGRKTAALISASIEAGAVLATEDEAIIGAYKAFGWD
                     LGIAFQLNDDLLGIWGPEVSTGKEPTDVPRKKKTLPVIYAFEHGSVDDRARLVALYAN
                     GRLLPNHVQEIVAILERSGAREYTRDQARLHRDRALAALEAAGTLEREPRERLEEIVR
                     SVISA"
BASE COUNT          708 a         1747 c         1629 g          750 t
ORIGIN      
        1 cctggtggcg cgacaggtcg aagtcgcccc ggttgtggat gccctcgagc tccttccagc
       61 cgaacgggaa gcggtactcg acgtcgacgg ccttcttggc gtagtgcgcg agctcgtcgg
      121 gcgcgtgctc gcggaagcgg aggcgctccg gagtgacgcc gtattgcacg taccagtccc
      181 agcggcgcgg gagccactcc tcgaagatgc gcgccgcctc gtccgggcgg acgaagtact
      241 gcatctccat ctgctcgaac tcgcgcatcc gaaacacgaa gttgcccggg ctgatctcgt
      301 tgcggaagct cttgccgatc tgggcgatcc cgaacggaag cttcttccgg gacgacccct
      361 ggacgttctt gaagttgacg tagctgccct gcgccgtctc cgggcggaga tagaccgcgt
      421 tcgagtcctc ctcgatcggg cccatgtgcg tcttgaacat caggttgaac tgacgcggcg
      481 cgctcagcgg cccgccatcg accgggcact tgaggcccag tcgctccacg atccccggat
      541 cgcgcaggtc cgatgccgac aggttctcgc cgccgggcag ctcgtcgagg cggaagcgtc
      601 gatggtccgt ctggcactcg accagtgggt cgctgaacga cccgacgtgg cccgacgtca
      661 cccagacctg cgggtgcatc aggatcccgg catcgagccc gacgatgtcg ttgcgctcct
      721 ggaccatcgc ccgccaccag gcgcgcttga cgttgttctt gagctcgacc ccgagcggcc
      781 cgtagtccca cacggcgttg atgccgccgt agatctcgga gctcgggtag acgaagccgc
      841 gccgcttcgc gagcgagacg atcgtgtcga gctcggccac ggcgggaccc gggtcggtcg
      901 tagtggggtg cgtcacgcgt gaggactccc gtgggcggcc ggccgtggaa tggacggtcg
      961 agggttgcgt tggcggccga tgctagcaga ggctgcggcg cgccttgcca caacgtcggt
     1021 ccgccccgat gatcgacgcc atggacccga gcccgccgcc gccccagcga cgcgaatcgc
     1081 tggtctcgag cgagcaatcg gcgcgcgagc tcgcgggcga gctcggcggc gcgatgaacg
     1141 cggtgatcct cgagccgctg gtcgcagacg cggtcgaagg catccggttc acgctgctcg
     1201 ccgtggtgat ggtcccgatc gggatcgtcg tgctcatcct gagcttcttc gtgtacttcg
     1261 gggcgttcct cgcgctcggc tcgccgaccg gctcgctggg cgcgatcgtc accggtgtct
     1321 ggctcatcgg aacgctggtg gcgcttgtcg tcgtgtaccg agcggtctcg cgtcggatgc
     1381 tcggtcgggg ccgcctcctg ggagcccggc ccgctccacc gaaggcggcc tttgccggtg
     1441 gcgcgctatc ggagctgctc ggcggcgagc cccaggcggc cgtggcggcc attgcgcccg
     1501 cgccatcgct tgccgagctt gacgcacgcc tcgcgccgat gtctccgccc acatcggcgc
     1561 ccaccgtgcc gcccacagcg gcgggcgcga cgccatcgca gcaggacgcg ccggtctaga
     1621 gcgccagcgt caggacgacc tcgccctcgt ggatctggcc tgtccgaacg aatccgtacc
     1681 gctcgtagaa cgggcacggg ctgccctcgc cctggccgca gctggtcagc agctcgacgg
     1741 cgccgggacg gtccttgagg taggcgcgga gcgcgtcgat cgtcgccgag ccgtagccac
     1801 ggcgctggtg gcgttgatcg atcagcaggc gccagaggaa gtacggcccg acgagcgtcg
     1861 ggtccacggc caggacctcc gccgcgatcc cgtcgctgag catcgcgaag ccaacgatct
     1921 gggggccgtc gtacacggcc cagtagcgcg cgcacgcctc cgggtacagc actgcctcgt
     1981 ccagcgacgc ctggacggtc gcgacgaact gctcctgatc gggcgcaacg cgaagcgcga
     2041 ccgccgctgt tcgatcctcg tccgtgacga tgtcacggag ctccacggtc atcgacggtg
     2101 gccagatcga ggggtctagg aggcctcggc gcccagcgcg tcggcgatcg actggatggt
     2161 ctggcgctcc caattgctga tgtccgtcgc gccgatgccg agcacgccgc cctcgcggac
     2221 cgctgccgcg acggcctgcg cggccgcgag gtaccaggct cgaatggcct ccgcctccgc
     2281 cggctcgagc tcgaccgcga tgatcgcgcc ggctcgacga gcctgctcga gcccgtccgt
     2341 gtatgcgtcg ccgctcacgg gctcgacgcc cacggcgagg agcgcgccga tgtccgagac
     2401 gagctggccg aggatgtcgt cggggccggc gccggtggcg gcatcggacg cgaactgcac
     2461 gaacgcgttc agctcctttt cggactgccc gcccgagccc gacgaggcgc tcaccgctcg
     2521 ggcgatcgcg ggcgctgctg ccacgagggc agcccagttc gtggccgaga gatctgcttt
     2581 cgtgctcatg gcctgagtgt acccacggcc tcccgttcgg cggcgcgttc ggccgctccg
     2641 gcgcgcacct cgtcgaggaa ggcgagcgag cgcggccgcc gatccagcga gtacgccagg
     2701 aactcgcgca tcgcgatctc gacgtcgcgc tccgtctccg gtcgtacccg cagcgcggcg
     2761 agggcctcga cgtcctggcg ctggtacgcc ttgagcagct tcagcgcctc gagcgagagg
     2821 ccggcgcgct catacggcgg gccggggcag cgctcacaaa gcacgccgcc cagtggcggc
     2881 atccagcgat agcgctcgtc ggactcgatg agccggccgc actcgacgca gcggtcgacc
     2941 tccggtcgga cgccgagctc gtcggcgagg tgcatctcga accaccgggc gacccgcccg
     3001 ggcgccatgc cggcgtcgag gatctcgtag gcgcgcttga gcaggaggta caccgactct
     3061 gcgccgtggc gctcctcgag cgtcccgttg gcgagctcgg caaggtacga cgccgtcccg
     3121 aacgacacga ggtcgtcgcg catgcgcagc cacggatgga tgacctcgac ctgggtgacg
     3181 acctcgaagg tccttccctg ggccagcgcg aggcgcagct cggcgaacgg ttcgaggctg
     3241 ccgccgatgc gcgacttctg ccgtcgaatg cccttcgcga tcgccttgat cttgccgccg
     3301 cccggcgtga tcagggtcag gatccgatcc gcttcgccat agtcgaagcg cgacagcacg
     3361 atcgcctcgg tgacgtagcg acgcggggcg ggcatcgact gacggtacac ccgcgcccgc
     3421 tccggcgcgc gagcgcggcc caagctcgat cgcggcccgc tgcgcatccc ggtcctccgt
     3481 cgacgtgcga tccaatcaca cgtcgggcga cgcacggcac cacgactttc gttcgacctc
     3541 cagactttgg attggatcgc acatctgacc gccgaacgcg cgatttcgat ccctcggacc
     3601 gggtcacggt cgccaactgc ccgccatctc acccattcga acgtcgttgt tgcaatccaa
     3661 tcgcatggcg acggccgaag cgggcccccg gggccaggcg ggcgcgcgaa cgcgctactt
     3721 cggtagcgca cgtttgttga tcgcgggcta cactccgcct cgatggccct ctcgaccagc
     3781 cccgacatcg ccgagctcat cgccgacacc gaggctgaga tcctccgcct cgtcgaaggc
     3841 tcggacccgt cgaccgccgg cctgtacgag atgtgccgct accacctcgg cctcgacggc
     3901 tcgggctcga ccggcaagcg gatgcgtccg ctgctcggcc tgctcgccta ctcctcgatc
     3961 accggtgatc accgtcgcgc gctgcccggc gccgccgcgg tcgagctggg gcacaacttc
     4021 agcctcgtcc acgacgacat cgaggacgac gaccgcgagc gccgccatcg acccacgctc
     4081 tgggcgttgc acggcgtgcc ccaggcgatc aacaccggcg acgtcctgtt cagcctcagc
     4141 cggatcgcgc tccatcgcct gaccgacctc ggcttcccgg acaagacggt gctccggctc
     4201 atgcgcctgt acgaccacac gtgcgtggcg ctgtgcgagg gccagtacct cgacatctgg
     4261 atggccggcc gcgacaccga cctctcggtc gagctgtact tcgacatgat cggccgcaag
     4321 accgccgcgc tgatctcggc gtcgatcgag gccggcgccg tcctggcgac cgaggacgag
     4381 gcgatcatcg gcgcctacaa ggcgttcggc tgggacctgg gcatcgcgtt ccagctcaac
     4441 gacgacctcc tcggcatctg gggccccgag gtatcgactg gcaaggagcc gaccgacgtg
     4501 ccgcgcaaga agaagacgct gccggtcatc tacgccttcg agcacggctc tgtcgacgac
     4561 cgggcgcggc tcgtcgcgct ctacgcgaac ggccgattgc tgcccaacca cgtgcaggag
     4621 atcgtggcga tcctcgagcg cagcggcgcc cgcgagtaca cccgcgacca ggctcgcctc
     4681 caccgcgacc gcgccctggc cgccctcgag gccgcaggca cgctcgagcg cgaaccgcgc
     4741 gagcgcctgg aagagatcgt ccgctcggtg atcagcgcgt agttcaaccg gtcaggactg
     4801 agctccgtga ccaacgcctg ttccgttgcc cgac
//
LOCUS       DATBSW010000357         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_140146, whole genome shotgun sequence.
ACCESSION   DATBSW010000357 DATBSW010000000
VERSION     DATBSW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140146"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VIF63_09825"
     CDS             <1..1273
                     /locus_tag="VIF63_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239720.1"
                     /translation="RSTWSIASLRAGGSAIASSARTATLVVLGSFAVAAPALALRPQL
                     FAIALFASTMLILAQRTAHPRRLWLIPFIAIAWANLHGSFPLIVVLVGLAWLDEVALL
                     REPTPAGQAQRRLPARLLGSTGLALFGAISAVATLVTPFGIDGWRYVENLARNPTITS
                     QVSEWRAPSPFDPAGAVFYLSLAVVLGVLAFRLRSDNRRPPARFLAPIVSVVVFGALG
                     VVTGRGLAWWAIVAPVAIVALQPGLKLADVKVPGLPTPRARTAREASISEERRSPLNT
                     IVMAVLILAGVALLPLWRPASTNGVPAATLSDAPQYLGAYLRTLTPGSGNPNVWAPQQ
                     WGSWIEFIAPNWKVAVDSRIELFPPDTWADNTLVAQVDGSWREIVDRYAVEVIVIDAH
                     DLALSTKLKGQPGWQLVYADDEAAIWRRESP"
     gene            1335..>2354
                     /locus_tag="VIF63_09830"
     CDS             1335..>2354
                     /locus_tag="VIF63_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239721.1"
                     /translation="MARRATLARQCSLVLAVLLVGCGGPAGPGPSAAPLSLPVHPDAT
                     ADADLFVVVTEHELDTLVGIDVEGRTLTRLAVLQPNPLRPVLDGGMASMRIEAAVLTG
                     PTSPDPLLWTTSMGAEAVVRTIHRASGELLAFDAPGAGDGWMPFLADGVLGWATFPAM
                     GDHRLIGAGGAFEIDLPGEVQFVVAGPGTGRVTAVVEPPSDDHDLDVVVIDLARKTVT
                     TLPIDPAYVGGIWADATTLAISDSGFSHRAILTWDVDASGSNPDAVAGLVRGPTLTTA
                     GPEPALVAGGEGFVVASTGGGDRSIVEVFDPHANESSRRFQLEKSCCITAMSVLGSTL
                     VIMENP"
BASE COUNT          300 a          898 c          753 g          403 t
ORIGIN      
        1 acgatcgacg tggtcgattg cctcgctccg tgccggcgga tcggccatcg cgagctccgc
       61 gcggaccgcg accctggtcg tgctcggctc gttcgccgtg gccgcgcccg cgctggccct
      121 ccgtccccag ctcttcgcga tcgccctctt cgcctccacc atgctgatcc tcgcccagcg
      181 caccgcccac ccgcgccggc tctggctgat cccgttcatc gcgatcgcct gggcgaacct
      241 ccacggcagc ttcccgctga tcgtcgtgct cgtcggcctc gcctggctcg acgaggtcgc
      301 gctcctgcgc gaacccaccc ccgccggcca ggcccagcgc cgcctcccgg caagactcct
      361 cggctcgacc ggccttgcgc tcttcggcgc catatcggca gtcgccacgc tcgtcacgcc
      421 gttcggcatc gacggttggc ggtacgtcga gaacctcgcc cgaaacccga ccatcaccag
      481 ccaggtctcc gagtggcgtg cgccctcccc gttcgacccc gccggcgccg tcttctacct
      541 ctcgcttgct gtcgttcttg gcgtgctcgc cttccggctc cgcagcgaca accgccgccc
      601 gccggcccgc ttcctcgctc cgatcgtgag cgtcgtcgtc ttcggggcgc tcggcgtggt
      661 gaccggtcga ggtctcgcct ggtgggcgat cgttgcgccc gtcgcgatcg tcgcgctcca
      721 gccgggcctg aagctggccg acgtcaaggt cccgggcctg cccacacccc gcgcccgaac
      781 cgcccgcgaa gcgtcgatca gcgaggagcg ccgctcaccg ctgaacacga tcgtgatggc
      841 cgtgctgatt ctcgcgggag tggcgctcct gcccctctgg cgcccggcct ccacgaacgg
      901 cgtccccgcc gcgacgctca gcgatgcgcc gcaatacctc ggtgcgtacc tcaggaccct
      961 cacccccggt tccggcaatc ccaacgtctg ggcgccgcag caatggggct cctggatcga
     1021 gttcatcgcc ccgaactgga aggtcgccgt cgactcccgc atcgagctct tcccgcccga
     1081 cacctgggcc gacaacaccc tcgtcgccca ggtcgacggc agctggcggg agatcgtcga
     1141 tcggtatgcc gtcgaggtga tcgtgatcga cgcgcacgac ttggccttga gtacgaagct
     1201 caagggccag cctggctggc aactcgtcta cgccgacgac gaggcggcga tctggcggcg
     1261 cgaatcgccg tagtccggtt gattgcgtgg aatcgcggag tgccgccgag cgacccctgc
     1321 gttacggttc cgccatggct cgccgtgcga ccctcgctcg ccagtgctcg ctcgtgttgg
     1381 ccgtgctcct ggtcgggtgc ggcggacctg cgggccccgg cccaagcgcg gcgccgctga
     1441 gcctgccggt ccacccagat gccaccgcag atgccgacct cttcgtggtc gtcaccgagc
     1501 acgagctcga cacgctcgtc ggtatcgatg tcgagggccg cacactgacg cgactggccg
     1561 tgctccagcc caacccgctt cgaccggtcc ttgacggcgg catggcatcc atgcgaatcg
     1621 aggcggccgt gctgaccggc cccacctcgc cggacccact gctctggacc acgtcgatgg
     1681 gtgccgaagc cgtcgtccgg acgatccacc gcgcgtctgg tgagctgctc gcattcgatg
     1741 cgcccggcgc gggcgacggc tggatgccgt tcctcgccga tggggtgctc gggtgggcga
     1801 cctttccggc aatgggcgac catcggttga tcggcgccgg cggcgccttc gagatcgacc
     1861 ttccggggga ggtccagttc gtcgtggctg gaccggggac cggtcgggtc accgccgtcg
     1921 tcgagccccc gtccgacgac catgacctcg acgtggtcgt catcgacctt gccaggaaga
     1981 ccgtgaccac cctcccgatc gatccggcgt acgtcggcgg catctgggcc gacgcgacga
     2041 cgctcgcgat ctcggactct ggcttttcac atcgcgcgat cctgacctgg gacgtcgatg
     2101 catccggctc caaccccgac gcggttgccg ggctcgtccg ggggccgacg ctgacgacgg
     2161 ccgggcccga gccggcgctg gtcgccggcg gcgagggatt cgtcgtcgcc tcgacgggtg
     2221 gaggcgatcg gtcgatcgtc gaggtcttcg acccccatgc gaacgaatct tcccgcaggt
     2281 tccagctcga gaagtcctgc tgcatcaccg cgatgtccgt gctgggttcg acgctcgtca
     2341 tcatggagaa ccca
//
LOCUS       DATBSW010000358         4199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_245828, whole genome shotgun sequence.
ACCESSION   DATBSW010000358 DATBSW010000000
VERSION     DATBSW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245828"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VIF63_09835"
     CDS             <1..742
                     /locus_tag="VIF63_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8239722.1"
                     /translation="RASRVVYDRADSCIARAGADAYAWSSLLDGARWFHTTGITPALS
                     GPAAEATLAAVRAARAAGATVSVDLNYRAKLWSWGAAAGEVMAGIVAEADVLIGNEED
                     ADKVFGIRAPGSVVAEGRVDPAGYASVAAQLADRFPKLRTIAFTQRGSISASENTWSG
                     VIWTADAFHVAKSYRIAPIVDRVGAGDAFAAGLIYALLDERPLEAALEFAVAASCLKH
                     SIRGDVNQVSVAEVDALAGGSGSGRVER"
     gene            742..1428
                     /locus_tag="VIF63_09840"
     CDS             742..1428
                     /locus_tag="VIF63_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239723.1"
                     /translation="MLSRRQQMLVAAVDAGVLPLFYTPDADRALRITSALREGGARVI
                     EFTDRGPGAWRAFTALAEEAARHDPEAILGAGSIRDAEAADRFIASGARFIVGPSFSA
                     DVARLCNRRRIPYGPGCATPTEIVTAEESGVELVKLFPGDSLGPGFLKAIRGPLPETQ
                     VVVTGGVQATEASVGEWIGAGAACLGFGSALVTKAHTDPASDADPATLTPAVAHLIAL
                     VAAARRSTQS"
     gene            1425..2927
                     /locus_tag="VIF63_09845"
     CDS             1425..2927
                     /locus_tag="VIF63_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8239724.1"
                     /translation="MTPAITAREYENLIDGKWAGAADGDTFERMSPAHDVTVGRYPRA
                     GVADLERAVGAARRAFDDGPWPTMPGVERARILNRVAEAIRADADDLAYIEALESGKP
                     VTQARDEIVSSADLWEYAATLCRHTYGDTYNTLGASMFGMVLREPVGVVGMITPWNFP
                     LLIISQKLPFALAVGCTAVVKPAEITPGTTLRLARMAQEAGLPDGVLNVVTGSGRVIG
                     TPLAAHPDVDMISFTGSTDVGRRVIDASKSNLKKVELELGGKNPQLVFADADLDAALD
                     AVVYGVYFNMGECCNSGSRLLVQRSIADEFIARVVERSRTVPVGDPLDPKTKVGAIAT
                     DEQFDKIIELVDSGRAEGADLLLGGGRLETERGRFMAPTVFSGVATTMRIAREEIFGP
                     VLSVLTFDTPEEAARIANATPFGLSAGVWTRDLDTAMGMSRAIRAGTIWVNTFLDGYP
                     ELPFGGYKESGIGREFGRFSVDAFTELKSVQMHLGPRTSWWMPRPGAPSK"
     gene            2924..>4199
                     /locus_tag="VIF63_09850"
     CDS             2924..>4199
                     /locus_tag="VIF63_09850"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8239725.1"
                     /translation="MSRLVPVLSAAEAVGRVPTGATVSVSSSSGLGCPDEVLRALGER
                     YAATGEGGDLTLLHPIAAGDMYGIDGVDHLTAPGQIRRIIAGSYPSGPSSAAPPKIVA
                     AIESNAIEAYNIPSGILFQLHRASAVGQPGVLTEVGMDTFMDPRRHGGRMNAKTTEDI
                     VQVVDFDGREWLYLRSIPVDVAIIRATTADEYGNLTSEEEGAPLGVLDQALAAHNNGG
                     FVIAQVKRLARGRTLPAQAVRVPGIVVDAIVLAPDQLQTTQTQFLPEAAGLYRAPSSG
                     LELAEWGSAKVIARRAAMELRDGEAVNLGFGISAFVPRILLEEGLDGAVTWVIEQGAV
                     GGVPFLGFAFGCAQNPDALMASPDQFQYLQGGGFDRAMLSFLEVDADGSVNVSRLAER
                     PHVTAGVGGFVDITARAKRIVFSGYFTAGAQIA"
BASE COUNT          581 a         1510 c         1487 g          621 t
ORIGIN      
        1 gcgtgccagc cgggtcgtct acgaccgggc cgactcctgc atcgcacggg ccggcgccga
       61 cgcgtacgcg tggtcgtcgc tcctcgatgg tgctcgttgg ttccacacca ccggcatcac
      121 gccggcgctg tctgggccgg ccgccgaggc aaccctggcg gcggtgcggg ccgctcgggc
      181 agccggtgcc accgtctcgg tcgacctcaa ctaccgcgcg aagctctgga gctggggtgc
      241 tgccgcaggc gaggtcatgg ccgggatcgt ggccgaggcc gacgtcctca tcggcaacga
      301 ggaggacgcc gacaaggtgt tcggtatccg agcgcccgga agcgtcgtgg ccgagggccg
      361 cgtcgatccg gccgggtacg cgtcggtcgc ggcgcagctc gccgaccggt ttccgaagct
      421 tcgcaccatc gccttcacgc agcgcgggtc gatctcggcg tccgagaaca cctggtccgg
      481 tgtgatctgg acggcggacg ccttccacgt cgcgaagtcg tatcgcatcg ccccgatcgt
      541 ggatcgcgtc ggcgccggcg acgccttcgc cgccgggctg atctacgcgc tgcttgacga
      601 gcggccactc gaggcggccc tcgagttcgc ggttgccgcg tcgtgcctga agcactcgat
      661 ccgcggcgac gtgaaccagg tgtccgtcgc cgaggtcgat gcgctggcgg gcggctccgg
      721 ctcggggcgg gtggagcggt gatgctctcg cgccgccagc agatgctggt cgcggccgtc
      781 gatgccgggg tgctgcccct cttctacacg cccgatgccg accgcgccct ccggatcacc
      841 tcggccctgc gcgagggtgg agcgcgggtg atcgaattca ccgaccgcgg tcccggcgcg
      901 tggcgagcct tcaccgcact cgcggaggag gcggccaggc acgacccgga ggccatcctc
      961 ggcgccgggt ccatccgcga cgccgaggcg gccgaccgct tcatcgcgtc gggcgcccgg
     1021 ttcatcgtcg gtcccagctt ctcggccgac gtcgcccgcc tgtgcaatcg ccggcgcatc
     1081 ccgtacgggc ccggctgcgc cacgccgacg gagatcgtga cggccgagga gtcgggggtc
     1141 gagctcgtca agctgttccc cggcgacagc cttggccccg gcttcctcaa ggcgatccgc
     1201 ggcccgctcc cggagacgca ggtcgtcgtg accggcggcg tccaggcaac cgaagcgagc
     1261 gtcggcgagt ggatcggtgc gggcgccgcg tgcctgggct tcggctcggc gctcgtcacg
     1321 aaggcgcaca cggatccagc ctcggatgcc gatcccgcca cgctgacgcc cgccgtcgcc
     1381 cacctcatcg ccctcgtcgc cgccgcccga aggagcaccc agtcgtgacc ccggcaatca
     1441 ccgctcgcga gtacgagaac ctcatcgatg gcaagtgggc cggtgccgcg gacggcgaca
     1501 ccttcgagcg aatgagccct gcccacgacg tgaccgtggg tcggtacccg cgggccggcg
     1561 tggcggacct cgagcgagcg gttggcgcgg cacggcgcgc gttcgacgac ggcccgtggc
     1621 cgacgatgcc cggcgtggag cgagcccgga tcctcaaccg cgtcgccgag gccatccggg
     1681 ccgacgcgga cgacctcgcc tacatcgagg cgctcgagag cggcaagccg gtgacccagg
     1741 cgcgcgacga gatcgtgtcg tccgccgacc tctgggagta cgccgcgacg ctgtgtcggc
     1801 acacgtacgg cgacacctac aacaccctgg gcgcctcgat gttcgggatg gtcctccgcg
     1861 agccggtcgg cgtcgtgggc atgatcaccc cgtggaactt cccgctcctg atcatcagcc
     1921 agaagctgcc gttcgccctc gccgtcggct gcacggccgt ggtcaagccg gccgagatca
     1981 cgccggggac cacgttgcgc ctcgcccgaa tggcccagga ggcggggctc ccggatgggg
     2041 tgctgaacgt cgtcaccggc tcggggcgcg tgatcggcac gccgctggcg gcccacccgg
     2101 acgtcgacat gatcagcttc acgggctcga ccgacgtcgg ccggcgggtc atcgacgctt
     2161 ccaagagcaa cctcaagaag gtcgagctcg agctcggggg caagaacccc cagctcgtct
     2221 tcgccgacgc cgacctcgac gccgccctgg acgccgtggt ctacggcgtc tacttcaaca
     2281 tgggcgagtg ctgcaacagc ggcagccgtt tgctcgtgca gcgctcgatc gccgacgagt
     2341 tcatcgcgcg agtcgtcgag cggtccagga ccgtgcccgt cggcgatccg ctcgacccga
     2401 agaccaaggt cggggcgatc gcgaccgacg agcagttcga caagatcatt gagctcgtcg
     2461 actccggccg tgccgagggc gccgacctgc tcctcggcgg cggccgcctc gagaccgagc
     2521 ggggccggtt catggcgccg accgtgttct ccggcgtggc gacgacgatg cgcatcgcgc
     2581 gcgaggagat cttcggcccg gtcctgtcgg tgctgacctt cgacaccccc gaggaggcgg
     2641 cgcggatcgc gaacgccacg ccgttcgggc tctcggcggg ggtctggacc cgcgacctcg
     2701 acacggcgat gggcatgagc cgcgcgattc gggccggcac gatctgggtc aacacgttcc
     2761 tggatgggta cccggagctg cccttcggcg gctacaagga gagcggcatc gggcgcgagt
     2821 tcgggcggtt cagcgtggac gcgttcacgg agctcaagtc ggtacagatg cacctcgggc
     2881 cgcgaacgtc gtggtggatg ccgcgcccgg gggctccttc gaagtgagcc gtctcgtgcc
     2941 ggtcctgtcg gcggccgagg ccgtcgggcg cgtcccgacc ggagccacgg tttcggtcag
     3001 ctcgtcgagc ggactcggct gccccgacga ggtcctgcgc gccctcggcg agcgatatgc
     3061 ggcgacgggg gagggtgggg acctgacgct cctccatccg atcgccgccg gcgacatgta
     3121 cggcatcgac ggcgtcgacc acctgacggc accgggccag atccgccgga tcatcgccgg
     3181 ctcgtacccg agcggtccgt catccgccgc gccgccgaag atcgtggccg cgatcgagtc
     3241 gaacgcgatc gaggcgtaca acatcccgtc ggggatcctg ttccagctcc atcgggcgtc
     3301 ggccgtcggc cagccgggcg tcctcaccga ggtgggcatg gacacgttca tggacccgcg
     3361 tcgccacggc ggtcgcatga acgccaaaac gaccgaggac atcgtgcagg tggtcgactt
     3421 cgacggccgc gagtggctct acctgcggtc catccccgtc gatgtcgcga tcatccgcgc
     3481 gacgacggcc gacgagtacg gcaacctcac gagcgaggag gagggcgcgc cgctcggcgt
     3541 gctcgaccag gccctcgccg cacacaacaa tggcggcttc gtgatcgccc aggtcaagcg
     3601 cctcgccagg ggccgaacgt tgcctgccca ggctgtccgc gtgcccggga tcgtggtcga
     3661 cgcgatcgtg ctcgcccccg atcagctcca gacaacccag acccagttcc tacccgaggc
     3721 agccggcctc taccgcgccc cgagctctgg cctcgagctc gccgagtggg gctccgcgaa
     3781 ggtgatcgcc cggcgcgcgg ccatggagct tcgcgacggc gaggcggtga acctgggctt
     3841 cggcatctcc gcgttcgtgc cgcgcatcct gctcgaggag ggcctcgacg gcgccgtcac
     3901 gtgggtcatc gagcagggcg ccgttggcgg cgtgccgttc ctcgggttcg ccttcggctg
     3961 tgcgcagaac ccggacgcgc tcatggcgtc gcccgaccag ttccagtacc tccagggcgg
     4021 cggcttcgac cgggcgatgc tctcgttcct cgaggttgac gccgacggca gcgtcaacgt
     4081 ctcacggctg gcggagcggc cccacgtcac cgccggggtg ggtgggttcg tggacatcac
     4141 cgcccgcgcg aagcggatcg tcttctccgg ctacttcacg gcgggcgccc agatcgccg
//
LOCUS       DATBSW010000359         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_845232, whole genome shotgun sequence.
ACCESSION   DATBSW010000359 DATBSW010000000
VERSION     DATBSW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845232"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VIF63_09855"
     CDS             <1..412
                     /locus_tag="VIF63_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8239726.1"
                     /translation="PDQDGVKIFEQFRDFVLEIAPRGVKVEVQNLGTARALRMDPNLP
                     VSKAAARALEATFGQAPVFIRSGGSIPVAAMFNDVLGLPITMLGLTNPDDNAHAPNET
                     MVLLNYETGLRTIATLWDDLGGMSPRDLKGGTTE"
     gene            409..1494
                     /locus_tag="VIF63_09860"
     CDS             409..1494
                     /locus_tag="VIF63_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8239727.1"
                     /translation="MKAGTQTAEPEGSWTLETGHGSMTPRTTIEVLNDIRAKVATGEL
                     ADYEPIPLGLAPLDRMLGGGLRPGELMLIGGAQGTGKTTMALQMARNIAASGQAYVLY
                     VNFEHDDADMLNRLIAMESALPNLPNTKPSTGAVKITDVRSEVIRSWARGAGDEPVDL
                     AANPRLKAALDRISRYGNNLYFLRGSATASTVENIRELIELHRARSGERRMLLIIDYL
                     QKVPQFPEPPTESEKVTYVVNGLKDIALSEKIPTIAIVAADKEGLKATRLRNHHLRGS
                     SAINYEADVILIINEKYQIVAKVNIEFNPYQAQRFRDWVIVSIEKNRSGADNVDVEFH
                     KHFEFSCFDPTGRPVQEKLIEERLYND"
     gene            1504..2517
                     /locus_tag="VIF63_09865"
     CDS             1504..2517
                     /locus_tag="VIF63_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8239728.1"
                     /translation="MRVLVTGGAGFLGRATVRALTEAGHGVRIVSRDAWPSGSLFDRL
                     GVEVVTGDAGQRSVIGAALQGMDGLVQAAATYRYDRGSADSMANNARLARTILEAAGD
                     AGTGKVIDISSLVVFALGRSPVSEDTPLTGPGDPSWADPYLRSKVESELVGRELEAKG
                     LPRVTIHPGTVIGPEDTAMGTSSGFVTNLLNGGLSVDSRAPWVDVRDVAWAIVLALGQ
                     PAGTRFALTSGVETHRNLAVLMDELTGLHPRRIFLSAGTTRFFARVNDAFGGRLAALP
                     DRGSIEWLLGNAPKVDTTRATRDLGLVFRPLRETLAATISWWAEHDSIDRSLAGKLAP
                     KPV"
BASE COUNT          430 a          840 c          872 g          384 t
ORIGIN      
        1 tcccgatcag gatggcgtga agatcttcga gcagttccgt gatttcgttc tggagatcgc
       61 accgcggggc gtgaaggtcg aggtccagaa cctcgggacc gcgagggccc tgcgcatgga
      121 tccgaacctg ccggtctcga aggccgccgc gcgggcgctg gaggccacgt ttgggcaggc
      181 tccggtgttc atccggtccg gtgggtcgat tccggtcgcg gcgatgttca acgacgtgct
      241 cgggctgccg atcacgatgc tgggcctgac caatccagac gacaatgccc acgcgccgaa
      301 cgagacgatg gtcctcttga actatgagac cggactgcgc acgatcgcca cgctatggga
      361 cgatctgggc gggatgtcgc cgcgagactt gaaggggggc acgaccgagt gaaggctggg
      421 acccagaccg cagagccgga aggcagctgg acgctcgaga ccggccacgg ctcgatgacg
      481 ccgaggacga ccatcgaggt cctgaacgac attcgggcga aggtcgccac gggtgagctc
      541 gcggactacg agccgatccc gctcggcctc gcgccgctcg atcggatgct cggtggcggc
      601 ctgcggccgg gcgagctcat gctcatcggc ggcgcccagg gcacgggcaa gacgacgatg
      661 gcgctccaga tggcgcggaa catcgccgct tcgggccagg cgtacgtgct gtacgtgaac
      721 ttcgagcacg acgacgcgga catgctcaat cggctgatcg cgatggaatc cgcgctgccg
      781 aacctgccga acacgaagcc ctcgacgggc gcggtgaaga tcaccgacgt ccgctcggag
      841 gtcatccgga gctgggcccg gggcgccggc gacgagccgg tcgacctcgc cgccaatccc
      901 cggctcaagg cggctctgga ccggatctcg cgctacggca acaacctgta cttcctccgc
      961 ggttcggcaa ccgcgagcac ggtcgagaac atccgcgagc tgatcgagct ccaccgcgcc
     1021 cgatccggcg agcggcgcat gctcctgatc atcgactacc tccagaaggt gccgcagttc
     1081 ccggagccgc cgaccgagtc cgagaaggtg acgtacgtcg tcaacgggct caaggacatc
     1141 gcgctgtccg agaagatccc gacgatcgcc atcgtcgccg cggacaagga aggcctcaag
     1201 gccacccgcc tgcgcaacca ccacctgcgc ggctcgtcgg cgatcaacta cgaagccgac
     1261 gtgatcctga tcatcaacga gaagtaccag atcgtcgcga aggtcaacat tgagttcaac
     1321 ccgtaccagg cgcagcgctt ccgcgactgg gtcatcgtgt cgatcgagaa gaaccggagc
     1381 ggcgcggaca acgtcgacgt cgagttccac aagcacttcg agttttcctg tttcgacccg
     1441 accggtcgac ccgtgcagga gaagctcatc gaagagcggc tctataacga ttaggctcgc
     1501 gtgatgcgag tcctcgtcac cggtggcgcg gggttcctcg ggcgcgcgac cgtgcgggcg
     1561 ctgaccgaag ccggtcatgg cgtgcggatc gtgtcgcgcg acgcctggcc cagcggctcg
     1621 ttgttcgacc ggctcggggt cgaggtcgtc accggcgatg ccggccagcg atccgtgatc
     1681 ggcgccgcgc tgcaaggtat ggacggcctc gtccaggcgg ccgcgaccta tcgctacgac
     1741 cgaggatcgg ccgactcgat ggccaacaac gcgcggttgg cgcgcacgat cctcgaggcg
     1801 gcgggcgacg caggcacggg caaggtcatc gacatcagct cgctcgtcgt cttcgccctc
     1861 gggcgctcgc cggtcagcga ggacacgccc ctgaccgggc ccggcgatcc gagctgggcc
     1921 gatccgtacc tccggagcaa ggtcgaatcg gagctcgtcg ggcgggagct ggaggcgaag
     1981 ggcctgccgc gggtcacgat ccatcccggg acggtgatcg gccccgagga tacggcgatg
     2041 ggcacgagca gcgggttcgt gacgaacctc ctgaacggcg ggttatccgt tgacagtcga
     2101 gcgccgtggg tcgacgtccg cgacgtggcg tgggccatcg tcctcgcgct cggccagccc
     2161 gcgggtactc ggttcgcctt gacgtccggg gtcgagacgc atcgcaacct cgcggtcctc
     2221 atggacgagc tgaccgggct gcacccacgc cgcatcttcc tgagcgcggg cacgacgcga
     2281 ttcttcgcgc gggtcaacga cgccttcggg ggccggctgg ccgcgttgcc cgatcggggc
     2341 tcgatcgagt ggctcctcgg caacgcgccg aaggtggaca ccacgcgcgc gacgcgtgac
     2401 ctcgggctcg tcttccggcc gctccgcgag acccttgccg ccacgatcag ctggtgggcg
     2461 gagcacgatt cgatcgatcg gtcgctcgcc gggaagctcg caccgaagcc ggtctgatgg
     2521 ctgtgc
//
LOCUS       DATBSW010000360         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_175003, whole genome shotgun sequence.
ACCESSION   DATBSW010000360 DATBSW010000000
VERSION     DATBSW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175003"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VIF63_09870"
     CDS             complement(<1..275)
                     /locus_tag="VIF63_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239729.1"
                     /translation="MKLVVVEFMTLDGVMEAPGTEPHRSGRNAWALKTSDDAFEAYNR
                     DQVFDADALLFGRTTYNIWAAFWPTGPETGGMTERINGMPKYVVSKTL"
     gene            493..1866
                     /gene="mgtE"
                     /locus_tag="VIF63_09875"
     CDS             493..1866
                     /gene="mgtE"
                     /locus_tag="VIF63_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY8239730.1"
                     /translation="MPGGDAITTQATADLLAEVQALIHERERDGVLGMAELIGPAEWA
                     DLVPQLEPSEVAVLIQWLPDEEIAAILEELPPHSAASILRSLSANEAGLLLGEMDPDD
                     AADVVELLPDEAAAEILVRMKPEDAAELRALSGYDPESAGGIMTPEYVAVSKDATSAQ
                     AIAAIRRLVDEAETVNYVYVVDEERHLLGVLSLYRLLLSVDTTPVERLMAPSTVRVRA
                     STDQETAARILTDRNLLAIPVVDDDDHLIGIITEDDVADVLQAEATEDIERLGGSQPL
                     NLPYRLASVPLLVRKRVGWLMLLFIAQGYTGTVLRNFEGELAAVVALSFFIPLLIGTG
                     GNVGSQTVTLVVRAMALGEVAMRDIGWLVFKELRVGLVMGLVMGVAAIGQAWFLNVSS
                     DIATAVSISVIAICVWAATVAAALPLILRRAGVDPAVVSAPLISTLVDGTGLIIYFEI
                     ARAVLRL"
     gene            complement(1863..3008)
                     /locus_tag="VIF63_09880"
     CDS             complement(1863..3008)
                     /locus_tag="VIF63_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEY8239731.1"
                     /translation="MTERRSRFQLPSRRTAAPSTQPAQPAPEVSMEARPEDITAPVEQ
                     SVVDAAIYVDGARVDSPETVAGTAQRLRDRPGAMAWIGMFRPTEAQLRPVAEEFGLHE
                     VAVEDAVVAHQRPKSERYGETLFVVLRAATYLDALEEVEFGEIHVFVGPNFVLTVRHS
                     RTPDLGAVRHRMEGDPELLARGPEAVLYAILDSVVDGYAPVVAGLQKDIEEIEVQVFR
                     GDPTVSRRIYELSGEVIEFQRSTRPLHAMLEALVAGFAKYGTDEELQKYLRDVADHAT
                     TVTERVAGFRQSLTDILAVNATLVNQAQNEEVKRLTEANMAQNEEVKRISSWAAIIFA
                     PTLVGTIYGMNFDMPEMHWALGYPFALVLMGVVSVGLWFVFRARGWL"
BASE COUNT          452 a         1099 c         1084 g          505 t
ORIGIN      
        1 agcgtcttgg agaccacgta tttcggcatc ccgttgatgc gctcggtcat gcccccggtc
       61 tccggaccgg tcggccagaa ggcagcccag atgttgtacg tcgtccggcc gaacagcagc
      121 gcgtccgcgt cgaagacctg atcgcggttg taggcctcga aggcgtcgtc gctcgtcttg
      181 agggcccacg cgttccggcc gcttcgatgc ggctcggtgc cgggcgcctc catgacgcca
      241 tcgagcgtca tgaactcgac gacgacgagc ttcatccgac gaagcgggcg ggacggtgcg
      301 gcatcgcgcc agtttgcagt gcgccgccgc cgcccgctgc cgccctgctt gcgcagcaac
      361 ggctttcgga tcgatcgggc agagccccat tgacccactc gggggcgccg cctacgatcc
      421 ggctgcccaa gccctagcgc ggactcgagg gttccccgag ccacccccat gtgagcgacc
      481 ttcgggcgct gcatgcccgg aggtgatgcc ataaccaccc aggcgacggc cgacctgctg
      541 gccgaggtcc aggcgctcat ccacgagcgc gagcgcgacg gcgtcctcgg catggccgag
      601 ctcatcgggc cggccgagtg ggccgacctc gtcccgcagc tcgagccgag cgaggtcgcg
      661 gtcctcatcc agtggctgcc ggacgaggag atcgcggcca tcctcgagga gctgccgccg
      721 cactccgcgg cgtcgatcct gcgctcgctg tccgccaacg aggccggcct cctgctcggc
      781 gaaatggacc ccgacgacgc cgccgatgtc gtcgagctgc tgcccgacga ggccgccgcc
      841 gagatcctcg tccgcatgaa gcccgaggac gccgccgagc tccgcgccct gtccggctac
      901 gaccccgagt cggccggcgg gatcatgacc ccggaatacg tcgctgtctc gaaggacgcg
      961 acgtccgcac aggccatcgc cgcgattcgc cgcctcgtgg acgaggcgga gacggtcaac
     1021 tacgtgtacg tcgtcgatga ggagcgccat ctgctcggcg tcctgtcgct gtaccgcctc
     1081 ctgctgagcg tcgacacgac cccggtcgag cgcctgatgg cgccgtcgac cgtgcgcgtc
     1141 cgggcctcga cggaccagga aacggcggcg cggatcctca ccgaccggaa cctcctggca
     1201 atccccgtgg tcgacgatga cgaccacctg atcgggatca tcaccgagga cgacgtggcc
     1261 gacgtcctcc aggcggaggc cacggaggac atcgagcggc tcggcggttc gcagccgttg
     1321 aacctgccgt atcgcctggc gtcggtgccc ctgctcgtcc gcaagcgcgt cggctggctg
     1381 atgctgctgt tcatcgccca gggctacacg ggcacggtgc tccgcaactt cgagggcgag
     1441 ctggcggcgg tcgtcgcctt gagcttcttc atcccattgc tgatcggcac gggcggcaac
     1501 gtcggcagcc agaccgtgac gctggtcgtg cgcgcgatgg cgctcggcga ggtcgccatg
     1561 cgcgatatcg gctggctggt gttcaaggag ctgcgcgttg gcctcgtcat gggcctcgtc
     1621 atgggcgtgg ccgcgatcgg gcaggcctgg ttcctgaacg tctcgtcgga catcgcgacc
     1681 gctgtctcga tctcggtcat cgcgatctgc gtctgggcgg cgactgtggc cgcggcgctg
     1741 ccgctgatcc tgcgccgtgc gggcgtggac ccggcggttg tctcggcgcc gttgatctcg
     1801 acgctcgtcg acggcaccgg cctgatcatc tacttcgaga tcgcgcgcgc cgtcctgcgc
     1861 ctctagagcc aaccacgcgc gcggaacacg aaccacaacc cgacgctgac gacccccatc
     1921 agcaccagcg cgaacgggta acccagggcc caatgcatct cgggcatgtc gaagttcatg
     1981 ccgtagatcg tgcccacgag ggtcggcgcg aagatgatcg cggcccatga ggagatccgc
     2041 ttcacctcct cgttctgggc catgttggcc tcggtgaggc gcttgacctc ctcgttctgt
     2101 gcctggttca cgagcgtggc gttgaccgcg aggatgtccg tgaggctctg gcggaagccg
     2161 gcgacgcgct cggtcacggt cgtcgcgtgg tcggcgacgt cgcgcaggta tttctggagc
     2221 tcctcgtcgg tcccgtattt cgcgaagccc gcgacgagcg cctcgagcat cgcgtgcaag
     2281 ggccgggttg agcgctggaa ctcgatgacc tcgccggaca gctcgtagat gcggcgggac
     2341 acggtcgggt cgccgcggaa gacctggacc tcgatctcct cgatgtcctt ctgcagcccg
     2401 gcgaccaccg gcgcgtagcc gtcgacgacg ctgtcgagga tcgcgtacag gactgcctcg
     2461 gggccccgcg ccagcagctc gggatcgccc tccatgcgat ggcgaaccgc gccgaggtcg
     2521 ggtgttcggc tgtggcgcac ggtgagcacg aagttcggcc cgacgaagac gtggatctcg
     2581 ccgaactcga cctcctcgag ggcgtcgagg taggtcgccg cccggaggac gacgaacagg
     2641 gtctccccgt agcgttcgct cttgggccgc tggtgggcga cgaccgcgtc ctcgacggcg
     2701 acctcgtgca gcccgaactc ctcggcgacg ggtcggagct gcgcctctgt cggccggaac
     2761 atgccgatcc aggccatcgc gccgggccgg tcacgcaggc gctgagccgt accggcgacg
     2821 gtttccggcg agtcgacgcg tgccccgtcc acgtagatcg cggcgtccac gacgctctgc
     2881 tcgaccggcg cggtgatgtc ctcgggccgc gcctccatcg acacctcggg cgccggttgg
     2941 gcaggctgcg tcgagggcgc ggcggtgcgc cggctcggca gctggaaacg ggaccggcgc
     3001 tctgtcatcc cggcaaggct acgcgctcag gcggagatca ggtccagggc gaagtagacg
     3061 ccgacggcgc ggatgtgtgc gcgcgccagc cggtagacga cattcgccgg aaacggctgc
     3121 tggaccgtcg cgcggatcgc
//
LOCUS       DATBSW010000361         5513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_1162133, whole genome shotgun sequence.
ACCESSION   DATBSW010000361 DATBSW010000000
VERSION     DATBSW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162133"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1994)
                     /locus_tag="VIF63_09885"
     CDS             complement(90..1994)
                     /locus_tag="VIF63_09885"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY8239732.1"
                     /translation="MTRFQRLAAATVAAAFGLVVLGVVVRATDSGVACPHWPGCFEGQ
                     FLPGLDAGFQVWIEWIHRTVAALIGVLILAMAALAVIDHRDRRSILWPSLAAVLLVGF
                     QAWLGRETVRLGNSGESVTAHLAAAMILVGLLMYVLVRASYPARLTGAGSQRFTLLAA
                     FAAAATFAVLLFGSNVTARDAGLIFPDWPLMNGSLVPVAPDGQDDLAALYGVHALHRY
                     VAAIVLAILAAVTWVAWRARDAQPGLARLAAAILALYAAQVVVGALQIATLLAPWTQT
                     LHVALAALVWGGTVALAAGAYYEARIAAPATGSGAIEGSATTEAGTAEHGTPEGGRRE
                     SVRASICAYVALTKPRIIELLLVTTVPAMVLATREVPGIPMIDWVRLTFWTLVCGTLA
                     AGSANAINQYLERDIDQHMARTRRRPLPANEVTPERAVVFGIVLGAFSVGLMAWTVNL
                     LAAFLTLLAIGFYVIVYTVMLKRSTPSNIVIGGAAGALPPVIGWAAVTGNIALPALLL
                     FLIVFYWTPPHFWALALRIRGDYAAAGVPMLPVVRGIAETSRQIGLYTILLVALTAVF
                     AVVAQMGLIYAVAAAVLGALFLWRAWILWRQGTSPEASTAQAIRLYRYSITYLAVLFA
                     AVALDTIWRV"
     gene            2081..3091
                     /locus_tag="VIF63_09890"
     CDS             2081..3091
                     /locus_tag="VIF63_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239733.1"
                     /translation="MFTPATSALRATRVARSAAAAVLALALVACSPGQSPSPTGPSPS
                     GSAACATAPEPPANLEGWGAPATAPTILPILIAPSVVICGSNRVVFSFLDAKNVPMGA
                     PDRTVALKIFSLGRDPANPTTAVDGTFIWAIENQVGIYVANVAFPESGVYGAEFVTAV
                     GGGEPEKVRLTFDVQPTSPVVKVGDKAPASKTPTAADVGGDISHLSTDPKPDPTFYET
                     SVDQALAKHEPFVLVFATPKFCTSATCGPTLDHVKPFAARYPTVTFINVEPYQLKLVD
                     GVLQPVLDADLRLQAAPVTDDWHLVNEPTVYVVDRTGIVRANFELIFSEAELTTALDA
                     VK"
     gene            complement(3107..4480)
                     /locus_tag="VIF63_09895"
     CDS             complement(3107..4480)
                     /locus_tag="VIF63_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239734.1"
                     /translation="MKRTLGRLVVLGLFVFGAAPARAMPAVDAPSIAAPGAFAAAQAT
                     AIVPGSVNRSSLKLRATYLVNASIAVSTGVINVTTTIDARNDSGDDIDRVELNTIAPH
                     FGPITITTATVDGTPVTVAVGSQTLTVPLGGILPNGASMQVRIGYRATLTNSLGGSNW
                     LWTRSGGTLALYRWIPWISASRPFGPDHGDPFVTPSSPHVHVDLHLDKSMVLATGANV
                     ASTAPSKTWSIGLDNVRDLSIVLAPDFSVTKTSANGVSIRAYSRPGGVSGATLADLAR
                     KALIAEASRIGVAYPWPNFTVIETKGGVAMESPRSIWIPGNSSSSSLKYLVYHETAHQ
                     WFYGLVGNDQSHEPFADEAAADMLARSVLGTLRSSHCASDDLDRWTSAYSASCYYETI
                     YIQGANFLNRMRDKMGTTRFWNAMEAYVQANRHGLAGTKKLLDALRAASPVDILPNLK
                     ARFPTLY"
     gene            4669..>5513
                     /locus_tag="VIF63_09900"
     CDS             4669..>5513
                     /locus_tag="VIF63_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239735.1"
                     /translation="MVVFRATAVALLPVILAVSAAMPFAAPAAAAAIPIAAAPSDLQD
                     PLPSPGKAGPNARAAAQAKVNAALRAAAKDATSAGAYFDGTDVAVFLTTGDPAKARGK
                     LARLVPGDAKVRFERVKHSQKDLETVQQRINADLGAGRLEGRGIVSTAIDTRANTVVV
                     GASQVDDQLRSALVRAYGDSVVVVFEGPSQGGDAGCVSRTDCGPAKGGIRIVRTAGGY
                     CTSGPMVRVLGSNALRILTAGHCLALTGGTGTSKTWTHSGSGLGWSEFHTWGNGADAD
                     VGLVNP"
BASE COUNT          895 a         1893 c         1924 g          801 t
ORIGIN      
        1 gagcacgttg gatggggacg tgcaggctca tcggcggcgg gacgctcgtg gtgggtcgtc
       61 ggttccgcga ccgggcgctg ggccgattcc tagacccgcc agatcgtgtc gagcgcgacc
      121 gcggcgaaga gcaccgccag gtaggtgatc gaatagcggt agaggcggat cgcctgggcg
      181 gtcgatgcct cgggcgaggt tccctgccgc cacaggatcc aggcgcgcca gaggaacagc
      241 gcaccaagga ccgcggcggc gactgcgtag atcaggccca tctgtgcgac gaccgcgaac
      301 accgcggtga gcgccaccag gaggatcgtg tacagcccga tctggcggct cgtctcggcg
      361 atgccgcgca cgacggggag catcggcacg ccggcggctg cgtagtcgcc gcggatccgc
      421 agggccagcg cccagaagtg cggcggcgtc caatagaaga cgatcaggaa gagcaggagc
      481 gcgggcaggg cgatgttgcc cgttaccgcg gcccagccga tgaccggcgg aagcgcgccc
      541 gccgcgccac cgatgacgat gttcgagggc gtgcttcgct tcagcatcac cgtgtagacg
      601 atcacgtaga agccgatcgc cagcagggtc aggaacgcgg cgagcaggtt cacggtccac
      661 gccatcagcc cgaccgagaa cgcgccgagc acgatcccga acacgaccgc gcgctcgggc
      721 gtcacctcgt tcgcgggcag cggccggcgc ctggtgcgag ccatgtgctg gtcgatgtcg
      781 cgctcgaggt actggttgat cgcgttcgcc gagcccgctg cgagcgtccc gcagacgagc
      841 gtccagaacg tgagccgcac ccaatcgatc atcgggatgc ccggtacctc gcgggtcgcg
      901 aggaccatgg ccggcaccgt cgtgacgagg agcagctcga tgatccgcgg cttggtgagg
      961 gcgacgtagg cgcagatcga tgcgcgcacg gactcgcggc gcccgccttc gggcgtcccg
     1021 tgctctgcgg tcccggcttc cgtcgttgcc gaaccttcga tcgcgcccga tcccgtggcg
     1081 ggagccgcga ttctcgcttc gtagtacgcc ccggcggcca gcgcgaccgt gccgccccag
     1141 acgagcgcgg cgagcgcgac gtggagcgtc tgcgtccagg gcgcgagcag cgtcgcgatc
     1201 tggagcgcgc ccacgaccac ctgcgccgcg tagagggcga ggatcgcggc cgcgaggcgc
     1261 gcgagcccag gttgagcgtc acgggcccgc cacgcgaccc aggtcacggc ggcgaggatc
     1321 gccagcacga tcgcggcgac ataccgatgg agcgcgtgga cgccatagag ggcggcgaga
     1381 tcgtcctggc catcgggcgc gacggggaca agcgacccgt tcatgagcgg ccagtcgggg
     1441 aagatcaggc cggcgtcgcg cgccgtcacg ttcgagccga agaggagcac cgcgaacgtc
     1501 gccgcagcgg cgaacgcggc gagcagggtg aaccgctggc tcccggcgcc cgtcagtcgc
     1561 gccggatacg aagcgcgaac cagcacgtac atgagcaacc cgacgaggat catggccgcg
     1621 gcgagatgcg ccgtgaccga ctcgccgctg ttgcccagtc ggaccgtctc acgcccgagc
     1681 cacgcctgga acccgaccag gagcacggca gccagggacg gccacaggat cgatcgtcga
     1741 tcgcggtggt cgatcaccgc gagtgcggcc atcgcgagga tgagcacgcc gatgagcgcc
     1801 gctacagttc gatggatcca ttcgatccag acctggaagc cggcgtcgag gccgggcagg
     1861 aactgccctt cgaagcagcc gggccagtgc ggacacgcga cgcccgagtc ggtcgcgcgc
     1921 acgacgacgc cgagcacgac gagcccgaac gcggcggcga cggttgcggc agcgagccgc
     1981 tggaaacgag tcacgagtcc tccgggtggc tcgcggattc tagcgacctg cgtgaacctg
     2041 tccggggccg atttcaggat cgacggatac gcttggtctc gtgttcacgc cagccacctc
     2101 cgcgctccgc gccactcgcg tcgctcggtc cgccgcggcg gcggtccttg ctctcgcgct
     2161 ggtggcgtgc agcccgggcc aatcgccctc cccgaccggc ccgagtccat cgggatcggc
     2221 ggcttgcgcc accgcccccg agccgcccgc gaatctcgag ggctggggcg cgcccgccac
     2281 tgcgccgaca atcctgccga tcctcatcgc ccccagcgtg gtcatctgcg ggtcgaaccg
     2341 ggtggtgttc agcttcctcg acgcgaagaa cgtcccgatg ggtgcgccgg atcggacggt
     2401 cgcgctcaag atcttctcgc tcgggcgcga ccccgcgaac ccgaccacgg cggtcgacgg
     2461 caccttcatc tgggccatcg agaaccaggt cggcatctac gtcgcgaacg tggccttccc
     2521 cgagtccggc gtttacggcg ccgagttcgt gaccgccgtc ggcggtggcg agcccgagaa
     2581 ggtacgcctg acgttcgacg tccagccgac ctcgccggtc gtcaaggtcg gtgacaaggc
     2641 gcccgcgtcg aagactccga ccgctgccga tgtcggcggt gacatcagcc acctctcgac
     2701 cgatcccaag ccagacccga cgttctacga gacctcggtc gaccaggcgc tcgcgaagca
     2761 cgagccgttc gtcctggtct tcgccacgcc caagttctgc acgagcgcca cgtgcggccc
     2821 cacgctcgat cacgtcaaac cgttcgcggc gcgctacccc accgtgacgt tcatcaacgt
     2881 cgagccgtac cagctcaagc tcgtcgatgg cgtgctccag cccgtcctcg acgcggacct
     2941 caggctccag gccgcgccag tcaccgacga ctggcacctg gtcaacgagc cgacggtcta
     3001 cgtcgtcgac cgcacgggca tcgtccgcgc gaacttcgag ctgatcttct ccgaggccga
     3061 gctgacgacc gccctcgacg cggtcaagta gcgctcgccg gggagctcag tacagggtcg
     3121 ggaatcgggc cttgaggttc ggcaggatgt cgaccgggct cgcggcccgc agcgcgtcca
     3181 ggagcttctt ggttccggca agcccgtgtc gattcgcctg gacgtaggcc tccatcgcgt
     3241 tccagaagcg ggtggtgccc atcttgtccc gcatccggtt gaggaagttc gcgccctgga
     3301 tgtagatcgt ctcgtagtag cagctcgccg agtacgcgga cgtccaacgg tcgaggtcgt
     3361 cgctggcgca atggctcgat cggagggtcc cgagcactga ccgcgcaagc atgtcggcgg
     3421 cggcctcgtc ggcgaacggc tcatgcgact ggtcgttgcc gacgagcccg tagaaccact
     3481 gatgcgcggt ctcgtggtag acgaggtact tgaggctcga gctcgacgag ttgccgggga
     3541 tccagatcga tcgaggcgac tccatcgcca ccccgccctt cgtctcgatg accgtgaagt
     3601 tcggccacgg atacgcgacc ccgattcggc ttgcctcggc gatgagcgcc ttgcgcgcca
     3661 ggtcggcgag ggtcgccccg gacacaccgc ccggacgcga ataggcccga atgctgacgc
     3721 cgttcgcgct cgtcttcgtg accgagaagt cgggcgcgag gacgatcgac aggtcgcgga
     3781 cgttgtcgag cccgatcgac caggtcttcg atggcgccgt ggaagcgaca ttcgcgccgg
     3841 tggccaggac catcgacttg tcgagatgca ggtcgacgtg gacgtggggg ctgctcggcg
     3901 tcacgaacgg gtcgccatgg tcaggcccaa acggccggga agcgctgatc catgggatcc
     3961 agcggtagag cgcgagcgtg cccccggagc gggtccacag ccagttcgag ccgccgaggc
     4021 tgttcgtgag cgtcgcccgg tagccgatgc gcacctgcat cgaggcgccg ttcgggagga
     4081 tgccgccgag cggcaccgtg agcgtctggc tgccgacggc aacggtcacg ggcgtcccat
     4141 ccacggtcgc agtcgtgatc gtgatcggcc cgaaatgcgg cgcgatcgtg ttcagctcga
     4201 cccggtcgat gtcgtcgccg gaatcgttcc gcgcatcgat cgtcgtggtg acgttgatca
     4261 cgcccgtcga gacggcaatg ctcgcgttga cgaggtaggt cgcgcgaagc ttcaggctcg
     4321 accgattgac cgacccgggc acgatcgcgg tcgcctgggc ggcggcgaac gcaccgggcg
     4381 ccgcgatgga aggggcgtcg accgcgggca tggcgcgtgc cggcgcggcg ccgaaaacga
     4441 acagaccaag cacgacgagg cggccgaggg tgcgcttcat tgggacccat catgcagccc
     4501 cgggcgattc gtggcgatca acccgtcgtt tgccgtccca aggactgaag tcaggtgtcg
     4561 ctttccagca aggtttctgc acactgacgg tacatccgcg tactggtgcc tccacgccgg
     4621 ggccccatgc tggacattcg tcgaaccgaa aaggggattt gccggggagt ggtcgtcttc
     4681 agggctaccg ccgtcgcgct gctgccagtg atcctggcgg tgtctgccgc catgcccttc
     4741 gccgctccgg ccgccgcggc cgcaatcccc attgccgcag ccccgagcga cctccaggac
     4801 cccctcccgt cgccgggcaa ggccggaccg aacgcccggg cagccgccca ggcgaaggtc
     4861 aatgcggcgc tccgcgctgc ggcgaaggac gccacatccg ccggggcgta tttcgacggc
     4921 accgacgtcg ccgtcttcct cacgacgggt gatccggcaa aggcgcgcgg caagctggcg
     4981 cgcctcgtac ctggagacgc caaggttcgg ttcgagcggg tcaagcattc ccagaaggac
     5041 ctggagaccg tccagcagcg catcaatgcg gaccttggcg ccggtcgcct tgagggccgc
     5101 ggaatcgtga gcacggccat cgacacacgc gcgaacacgg tcgtcgtggg tgcgtcgcag
     5161 gtcgacgacc agctccgcag tgcgctcgtc cgtgcctacg gcgacagcgt cgtcgtggtc
     5221 ttcgaaggtc cctcgcaggg cggcgatgcc ggctgcgtct cgcggacgga ctgcggacca
     5281 gcgaagggcg gcatccggat cgtgcggacc gccggcggct actgcacgag cgggcccatg
     5341 gttcgcgtac tcggcagcaa cgcgctccga atcctgactg ccggccattg cctggcgctg
     5401 accggcggca cgggcacgag caagacgtgg acgcacagcg gctcgggcct cggctggtcc
     5461 gagttccaca cgtggggcaa tggcgccgac gcggacgtcg gcctggtcaa ccc
//
LOCUS       DATBSW010000362         5263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_422968, whole genome shotgun sequence.
ACCESSION   DATBSW010000362 DATBSW010000000
VERSION     DATBSW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422968"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..2061)
                     /gene="acs"
                     /locus_tag="VIF63_09905"
     CDS             complement(49..2061)
                     /gene="acs"
                     /locus_tag="VIF63_09905"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY8239736.1"
                     /translation="MAVEQPSRPSSPAQAHPEQFANALIETGLHEQRTFPPDPAFTAQ
                     ANAQADLYLEAETDYEEFWAKLARERISWFKPFEKTLEWDLPFARWFEGGELNVSYNC
                     VDRHVENGLGSKVAYHWIGEPGDTRTITYADLYREVQKTARALSALGVGKGDRVAIYM
                     PMIPELPIAMLACARIGAPHTVVFGGFSAEALSGRINDAEAKLVITADGGWRRGKAAP
                     LKPAVDEALLSTPSVHSVLVVQRLGDAAPDTTMVDGRDHWWHDIVEAQPPDCAPLPVD
                     SEHMLYLLYTSGSTAKPKGIFHSTAGYLLGTSYSHEMIFDVKPDDVYWCAADVGWVTG
                     HSYIVYGPLANATTGVMYEGTPDTPAWDRWWQIIEDYKVSILYCAPTAIRAFMKQGAE
                     HPAKHDLSSLRVLGSVGEPINPEAWLWYREHIGGSKAPIVDTWWQTETGQILITPLPG
                     VTTTKPGSATFPFPGIKADVVDGDGNSVPLGGGGYLVLKRPWPAMLRGIYGDPERYKA
                     TYWSRFPGMYFAGDGAKRDEDGYFWLLGRVDDVMNVSGHRLSTTEIESALVSHPAVAE
                     AAVVGGPDAVTGEAINCFVILRKGVEPSDELALELRNHVSTKISPIAKPKSLMFTPDL
                     PKTRSGKIMRRLLRDIAEGRQLGDVTTLADATIADTIRDNTGKAEE"
     gene            complement(2507..3436)
                     /locus_tag="VIF63_09910"
     CDS             complement(2507..3436)
                     /locus_tag="VIF63_09910"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8239737.1"
                     /translation="MPKLDLAFVGDVLETYALPIVLIVIVAFVLVRAARLFVHGIIKA
                     LMDREATEGTAQELSAVELKKRMDTLDGLGANVLRFFIIVIAGLMILRAFGLDIGPAV
                     AGLGVVGVAVGFGAQHLVRDYLNGALIMIENQFSKGDVIRAAGVSGTVEDFSLRRTTL
                     RDLDGVVHTVPNGEIQVASNLTRVWARINLDVIVAYGTDIDAATKVVNDVGKSMAADP
                     SWKRRVLEAPSVDRVAALGEYGVTLKILGTVRAPDQWAAAGELRKRLLAAFKKHSIEI
                     PRPQRVILSRDPSALPSTLPPPDESLAEPISAD"
     gene            complement(3518..4933)
                     /locus_tag="VIF63_09915"
     CDS             complement(3518..4933)
                     /locus_tag="VIF63_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8239738.1"
                     /translation="MPAQQPPTLKRQRQLDLYARAQRTMPGGTDSNFRAWGDDTVYVD
                     RGEGGRLWDIDQNEYIDLRMGYGPVILGHGDPRVDDYVNERMRRGVSFSLTSEDEVEA
                     MEILKGMTGWVGKARMTVSGTEATMHAMRVARGYTGRSKIVKFEGQYHGVHDYALISV
                     TPNDMSELGDEDNPVGLAWGRGIPEQVAKTMIPARFNRIDVLRRIFERDGHDIAAVIV
                     EPILGNAQAILPREGFHQEMRALTKEFGALLIFDEVKTGFRIAKGGAAEYFGVTPDLA
                     TYAKAIGNGYPAACFAGSDEVMSVLPDKVSHGGTYAGNRVAAAACVATLKILRDTNAL
                     ASIHQVGREVQAGLSEVISERGLPYVFTGHPAMFGIMFTEQAPTEYRDWANSDHDLYD
                     AVAMGMHARGAMPEPDSREPWFFCEAHTREDVVDRIVSIFSDSLDAALEARSHGEHAA
                     GVSAAQWSMGAGAGAVGQPAD"
     gene            complement(5075..>5263)
                     /locus_tag="VIF63_09920"
     CDS             complement(5075..>5263)
                     /locus_tag="VIF63_09920"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY8239739.1"
                     /translation="WPAQALTYKVGQLEIERLRREITLRDGPAFDLREFHDQLLGHGS
                     LPLATLTRELPNWVATPA"
BASE COUNT          750 a         1806 c         1826 g          881 t
ORIGIN      
        1 cccttcgcgt cgcccttgcc ctttttcagg aagccgaacg gcacccgatc actcctccgc
       61 cttgccggtg ttgtccctga tcgtgtcggc gatggtcgcg tcggcgagcg tcgtcacgtc
      121 ccctagctgg cgcccctcgg cgatgtcgcg caggagccgg cgcatgatct tgcccgagcg
      181 cgtcttgggc aggtccggcg tgaacatgag cgacttcggc ttggcgattg ggctgatctt
      241 cgtcgagacg tggttgcgca gctccagtgc cagctcatcg ctcggctcga cgcccttgcg
      301 caggatcacg aagcagttga ttgcctcgcc cgtcaccgca tcgggcccgc cgacgacagc
      361 cgcctccgct accgcggggt gcgagacgag ggcgctctcg atctcggtcg tcgaaagtcg
      421 atggcccgac acgttcatga cgtcgtccac gcggccgagc agccagaagt agccgtcctc
      481 gtcgcgcttg gcgccgtcgc cggcgaagta catccccggg aagcggctcc aatacgtggc
      541 cttgtaccgc tcgggatcgc cgtagatccc gcggagcatc gcgggccacg ggcgcttgag
      601 gacgaggtat cccccacccc cgaggggcac cgagttgccg tcgccgtcga cgacgtccgc
      661 cttgatgccc gggaacggga aggtcgcgct gccgggcttc gtcgtcgtga cgccgggcag
      721 cggcgtgatc aggatctggc cggtctcggt ctgccaccac gtgtcgacga tcggcgcctt
      781 gctgccaccg atgtgctcgc ggtaccagag ccaggcctcc gggttgatgg gctcgccgac
      841 cgagccaagc acgcggagcg aggacaggtc gtgcttcgcc gggtgctcgg cgccctgctt
      901 catgaaggcc cgaatcgccg ttggcgcgca gtacaggatc gagaccttgt agtcctcgat
      961 gatctgccac cagcgatccc acgccggcgt gtccggcgtg ccctcgtaca tgacgcccgt
     1021 cgtcgcgttg gcgagcgggc cgtacacgat gtagctgtgg ccggtcaccc aaccgacgtc
     1081 ggccgcgcac cagtacacgt cgtcgggctt gacgtcgaag atcatctcgt ggctgtagct
     1141 cgtcccgagc aggtagcccg cggttgaatg gaagatgccc ttcggcttcg cggtcgagcc
     1201 cgaggtatac agcaggtaca gcatgtgctc gctgtcgaca ggcagcggcg cgcagtccgg
     1261 tggctgggcc tcgacgatgt cgtgccacca gtggtcccgg ccgtcgacca tcgtcgtgtc
     1321 cggggccgca tcgcccaggc gctggaccac gagcacgctg tgcaccgacg gcgtcgagag
     1381 cagcgcctcg tcgaccgccg gcttgagcgg cgccgccttg ccccgccgcc agccgccgtc
     1441 ggcggtgatg acgagcttcg cctcggcgtc gttgatccgg cccgacagcg cctccgccga
     1501 gaagccgccg aagacgacgg tgtgcggcgc gccgatccgg gcgcaggcga gcatcgcgat
     1561 cggcagctcc gggatcatcg gcatgtagat ggcgacccgg tcgcccttgc ccacgcccag
     1621 ggcgctgagc gcacgcgcgg tcttctggac ctcgcggtag aggtccgcat aggtgatcgt
     1681 ccgggtgtcg cccggctcgc cgatccagtg gtacgcgacc ttgctcccga gcccgttctc
     1741 gacgtgccgg tcgacgcagt tgtacgagac gttcagctcg ccaccctcga accagcgggc
     1801 gaatggcagg tcccactcga gcgtcttctc gaacggcttg aaccagctga tccgctcgcg
     1861 tgcgagcttc gcccagaact cttcgtagtc cgtctcggcc tcgaggtaga ggtcggcctg
     1921 ggcgttggcc tgcgccgtga acgccggatc gggcgggaag gtccgctgct cgtgcagccc
     1981 ggtctcgatc agcgcgttcg cgaactgctc cggatgcgcc tgggcggggc tcgaaggtcg
     2041 actgggctgt tcgacggcca atgcagcctc cacgtccgtt gggcgccgat cctagcaagg
     2101 aacggcccat cgatggtcgt gcgcgatcgg ccgaggccgt cgtttgcgct cgggtcgata
     2161 gccatccgca gcgtcccgca gcgttcggct cggtgcggac cgccccgggg gccgcctcgc
     2221 caacgaaccg accgacgatg gtcacggcgt gatcatttct ggaggtctgg gcgccatttg
     2281 ctccgtggcc gagcgcctcg acgggccctg ctgggcgtca gcctggatac ctgagcgcga
     2341 atcgaccgcg ccgtcgacct cttgccaagc agctgcagcc cagatcttcc atttcctgcc
     2401 cgaaaacggc cgcgaagccc agaaaatgat cgcccggtga tcttcccgaa tggaatgggc
     2461 tcgatcgcgg ctccgtcgcg gctccggtcg cggctccggt cgcggctcag tcggcggaga
     2521 tgggctccgc caggctctcg tcgggcggcg ggagtgtgct cgggagcgcg ctcgggtcgc
     2581 gcgacaggat gacgcgctgc ggccgcggga tctcgatgct gtgtttcttg aatgccgcga
     2641 gcaggcgctt gcggagctcg ccggcggcgg cccactgatc cggcgcccgg acggtgccga
     2701 ggatcttgag cgtcacgccg tactcaccga gggcggcgac gcggtcgacg ctcggcgcct
     2761 cgagcacgcg gcgcttccag gatggatcgg cagccatcga cttcccgacg tcgttgacga
     2821 cctttgtcgc cgcgtcgatg tcggtgccgt aggcgacgat gacgtcgagg ttgatccgtg
     2881 cccagacacg ggtcaggttg ctcgcgacct ggatctcgcc gttgggcacg gtatggacga
     2941 ccccgtcaag gtcgcgcagc gtcgtgcgcc gcaggctgaa gtcctcgacc gtgcccgaga
     3001 cgccggcggc gcggatcacg tcgcccttgc tgaactggtt ctcgatcatg atcagggcgc
     3061 cgttgaggta gtcgcgcacg aggtgctgcg ccccgaaccc gaccgcgacg ccgaccacgc
     3121 cgagaccggc gacggccggc ccgatgtcga gcccgaacgc acgcaggatc atcagcccgg
     3181 caatcacgat gatgaagaac cggaggacgt tcgcgccgag cccgtcgagc gtgtccatgc
     3241 gcttcttcag ctcgaccgcg gagagctcct gggccgtgcc ctcggtggcc tcgcggtcca
     3301 tcagcgcctt gatgattccg tgcacgaaca ggcgcgccgc gcgcaccagc acgaacgcca
     3361 cgatgacgat caggacgatc gggagggcgt acgtttcgag cacgtcgccg acgaaggcga
     3421 ggtcgagctt gggcatggcg gaagggtagc cgacgaggat tccgcgccgc gtcgcggaag
     3481 aacgtacccg ccgcttgggg ctcgccctcg acgcgcttca gtcggcgggc tgtcccaccg
     3541 cacccgcgcc ggcgcccatg ctccactgcg ctgcgctgac gcctgccgcg tgctcgccgt
     3601 gcgagcgggc ctcgagtgcc gcgtcgagcg agtccgagaa gatcgagacg atccgatcga
     3661 cgacgtcctc gcgcgtgtgc gcctcgcaga agaaccacgg ctcgcgcgaa tccggctcgg
     3721 gcatcgcgcc gcgggcgtgc atgcccatcg cgactgcgtc gtacaggtcg tggtccgagt
     3781 tcgcccagtc gcggtactcg gtcggcgcct gctcggtgaa catgattccg aacatcgccg
     3841 ggtggccggt gaacacgtac ggcaggccgc gctccgagat gacctcggat agtcccgcct
     3901 gcacctcgcg cccgacctgg tggatcgagg cgagggcgtt cgtgtcacgc aggatcttga
     3961 gggtcgccac gcaggcggcg gctgcgacgc ggttgcccgc gtaggtgccg ccgtgcgaga
     4021 ccttgtcggg caggaccgac atgacctcgt cggagccggc gaagcacgcg gccgggtagc
     4081 cgttgccgat cgccttcgcg tacgtcgcga ggtccggcgt gacgccgaag tactcagcgg
     4141 cgccgccctt cgcgattcgg aaaccggtct tgacctcgtc gaagatcagc agcgccccga
     4201 actccttggt cagggcgcgc atctcctggt ggaagccctc gcgcgggagg atcgcctggg
     4261 cgttgccgag gatcggctcg acgatcacgg ccgcgatgtc atggccgtcg cgctcgaaga
     4321 tccggcgcag gacgtcgatc cggttgaagc gagccgggat catggtcttg gcgacctgct
     4381 ccgggatgcc ccggccccac gccagcccga ccggattgtc ctcgtcgccg agctcggaca
     4441 tgtcgttcgg ggtgaccgag atcagggcgt agtcatgcac gccgtggtac tggccctcga
     4501 acttcacgat cttgctgcgc ccggtgtagc cgcgggcgac gcgcatcgcg tgcatggtcg
     4561 cctcggtccc ggacacggtc atccgggcct tgccgaccca gcccgtcatc cccttgagga
     4621 tctccatcgc ctcgacctcg tcctccgagg tcagcgagaa gctcacgccg cggcgcatcc
     4681 gctcgttcac gtagtcgtcg acgcgcgggt cgccgtggcc gaggatcacc ggaccgtagc
     4741 ccatgcgcag gtcgatgtac tcgttctggt caatgtccca cagccggccg ccctcgccgc
     4801 ggtcgacgta gacggtgtcg tcaccccagg cgcggaagtt ggaatccgtg ccgccgggca
     4861 tcgtccgttg ggcacgggcg tagaggtcga gctggcgctg ccgcttgagc gtgggcggct
     4921 gttgggcggg cattgggctc cttgctgccg cgggcgcggc tttgggtcga atgaatcgtg
     4981 ccacgcgctc taggggcccg tcaaagtcgg gattcgcacc gccgtgccgg aatccgtgcg
     5041 aatcgcgcag tcgcgctgcc gggcggcgcc aggctcaggc gggcgtcgcc acccagttcg
     5101 gcagctcgcg ggtcagggtt gcgagaggca gcgagccgtg gccgaggagc tggtcgtgga
     5161 actcgcgcag gtcgaacgcc gggccgtcgc gcagcgtgat ctcccggcgc agccgttcga
     5221 tctcgagctg gccgaccttg tatgtcagcg cctgggccgg cca
//
LOCUS       DATBSW010000363         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_387446, whole genome shotgun sequence.
ACCESSION   DATBSW010000363 DATBSW010000000
VERSION     DATBSW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387446"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..404
                     /locus_tag="VIF63_09925"
     CDS             216..404
                     /locus_tag="VIF63_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8239740.1"
                     /translation="MGDVVIILIIILALAVIWRGPKTIPEIGRMFGRGVRNAREEIEK
                     TRGSDEPPPSAGSTGSQP"
     gene            complement(409..789)
                     /locus_tag="VIF63_09930"
     CDS             complement(409..789)
                     /locus_tag="VIF63_09930"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8239741.1"
                     /translation="MDHQDAMQMLKAITTAFDQHDLEGIMAHFADDAVFEGPRGAEPW
                     GTRIVGREAVAQAFAGRFAGIPDVRYQQDTHFVDGDRGASEWTLSGTTTDGTSIEVRG
                     CDLWTFRDGLVVKKDSFWKIRTPG"
     gene            940..>1676
                     /locus_tag="VIF63_09935"
     CDS             940..>1676
                     /locus_tag="VIF63_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY8239742.1"
                     /translation="MTTAVRRSRALALVVALVGVLGTAAPAAAAAPADFPAKDSRYHN
                     YDEMVAEIHAVETAHPDLVHLFSIGKSYQGRELWAAKVSDNVATDEAEPEAMIVAAHH
                     GDEHLSVEQGLYLFNVLLDDYGTDAKVKKLVDSREIWFVFMLNPDGAQYDLTGDPYRG
                     WRKNRQPNAGSTYVGTDLNRNYGYHWACCGGSSNYPNVKTYHGPKAFSTPETTAMANF
                     VKSRVIGGMQQIRTQISLHASGELVLYP"
BASE COUNT          297 a          574 c          542 g          263 t
ORIGIN      
        1 cacgcgagct cgtcgtcgct cgccggctcg ggggcgagat tcggcacggc cccgagcgac
       61 tcgagcaggg agatcccggg cccgaaccgg cgcggggtca gcgggtgggc aggcccgaag
      121 tcataggtcg tcgagcgcgg cccgaacacg agcagcggag gggcggtgga cacgagcggc
      181 acgatagccc gtcggcgcgg cgtaccatcc gcgacgtggg cgacgtcgtc atcatcctga
      241 tcatcatcct tgccctggcc gtgatctggc gaggtccgaa gacgatcccg gagatcggtc
      301 ggatgttcgg gcgtggcgtc cgcaacgcgc gcgaggagat cgagaagacg cgcggcagtg
      361 acgagccgcc cccgagcgcc gggtcgaccg ggagccagcc ctagcgcttc agccaggcgt
      421 tcggatcttc cagaaggaat ccttcttcac gaccaggccg tcgcggaacg tccacaggtc
      481 gcagcctctg acctcgatcg acgtcccgtc ggtcgtcgtt cccgaaaggg tccattcgga
      541 cgccccgcgg tcaccatcga cgaagtgggt gtcctgctgg tatcgaacat cgggaatgcc
      601 ggcgaatcga ccggcgaagg cttgggccac cgcctcgcgg ccaacgatcc tggtgcccca
      661 cggctcggca ccgcgtggcc cttcgaacac ggcgtcgtcg gcgaagtggg ccatgatccc
      721 ctcgagatcg tgctggtcga acgcggtcgt gatcgccttg agcatctgca tcgcgtcctg
      781 gtgatccacg actcctcctg accttcggca ttcgatcgtc ggatcgaagg tacctcccgg
      841 cgacacgtcc aggacaggga cgcgatcgtg acgcgccata gcggtcagtc cttttgggtt
      901 attcccgaac tttgtggtat cggtccatgc tgtaccggca tgacgaccgc ggtccgccgc
      961 tcccgtgccc tcgccctcgt cgtggcgctg gtcggcgtcc ttggcacggc ggccccagca
     1021 gccgcggcgg cccccgcaga cttccccgcg aaggactcgc gctaccacaa ctacgacgag
     1081 atggtcgcgg agatccacgc cgtcgagacg gcacacccgg atctcgtcca ccttttctcg
     1141 atcggcaaga gctaccaggg tcgcgagctg tgggcggcga aggtcagcga caacgtcgcg
     1201 actgacgagg ccgagcccga ggcgatgatc gtcgcggccc atcacggcga cgagcacctg
     1261 tcggtcgagc aggggctcta tctgttcaac gtcctcctcg acgactacgg caccgacgcg
     1321 aaggtgaaga agctcgtcga cagccgcgag atctggttcg tgttcatgct caaccccgat
     1381 ggcgcccagt acgacctcac cggcgacccg taccgaggct ggaggaagaa tcgccagccc
     1441 aacgccggca gcacctacgt cggcactgac ctcaaccgca actacggcta ccactgggcc
     1501 tgctgcggcg gatcctccaa ctacccgaac gtgaagacct accacgggcc gaaggcgttt
     1561 tcgacgccag agacgacggc catggccaac tttgtcaaga gccgggtgat cggcggaatg
     1621 cagcagatcc gaacccagat ctcgctccac gcgagcggcg agctggtcct ctaccc
//
LOCUS       DATBSW010000364         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_387500, whole genome shotgun sequence.
ACCESSION   DATBSW010000364 DATBSW010000000
VERSION     DATBSW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387500"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VIF63_09940"
     CDS             <1..588
                     /locus_tag="VIF63_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239743.1"
                     /translation="PAPLPLGVLAWPTAFDTPLEEATYFSSPPFRIPFTLVVGEPGWG
                     TGHLHADFFDLLRFDGVAHEQLPNRMLAFADSDYVIGVHGNVGVATLTPRAALDLLAE
                     RPDLTVSTREQVELFGLAGERLNVHSATGSNHVFGGAGGDLGIGPELDTRLAFLPLNG
                     RLLGVLVMAEADDLQAAWDQALRILDTVDLVTADH"
     gene            647..1021
                     /locus_tag="VIF63_09945"
     CDS             647..1021
                     /locus_tag="VIF63_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239744.1"
                     /translation="MLRRIALAAITAAVLAGCSLIDDLSRGDPIVEMEVWNQTLDDVA
                     LVDDDGRIINVPACGHADAALLRVDRVEIRMDAGRISSFASRGIRGPQFLVVVANGFD
                     SFPTNQRPAALPPCAGHPTVVP"
     gene            1050..>1560
                     /locus_tag="VIF63_09950"
     CDS             1050..>1560
                     /locus_tag="VIF63_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY8239745.1"
                     /translation="MTTHVDRPETSPVGDGPEPTRSPTIGEGDRVELLDYGFVRLDAS
                     MADDLSVANAARVSFARRKEELDDSDRGLIRFLMRDRHGTPFEHNSFRFHIRCPIFVA
                     REWMRHRIGCLTGETVVTFVDTNGHATPLLAKSLDALWQSWAEGERDGHAVDEGTASR
                     IRELAAMGVS"
BASE COUNT          241 a          551 c          502 g          266 t
ORIGIN      
        1 cccgcaccct tgcccttggg cgtgctggcg tggccaacgg cgttcgacac gccgttggag
       61 gaggcgacgt acttcagctc gccgcccttc cggatcccgt tcacgctcgt tgtcggcgag
      121 ccgggctggg gcaccgggca cctccacgct gacttcttcg acctcctgcg cttcgacggc
      181 gtcgcccacg aacagctccc gaaccggatg ctcgccttcg cggattccga ctacgtcatt
      241 ggcgtgcatg gcaacgtcgg cgtcgcgacg ctcacgccgc gagcggccct cgacctgctc
      301 gccgagcgcc cggacctgac cgtctccacc cgggagcagg tcgagctgtt cggcctcgcg
      361 ggcgagcggc tgaacgtcca ctcggcgaca ggcagcaacc acgtgttcgg tggcgccggc
      421 ggggacctcg gcatcggacc ggagctcgac acgaggctcg cattcctgcc cttgaacggg
      481 cggttgctcg gcgtcctcgt catggccgag gccgacgatc tccaagctgc ctgggaccaa
      541 gccctccgga tcctcgacac cgtcgacctc gtgaccgccg accactgaac ctcacccgcc
      601 gccgtacgtc gcgctacatc gacgcccagc gcgacgtctg cagggcatgc ttcgacgaat
      661 agcgctcgcc gcaatcacgg cggccgtcct cgcgggttgc tccctgatcg acgacctgtc
      721 ccggggcgac ccgatcgtcg agatggaggt gtggaaccag acgctcgatg acgtcgctct
      781 ggtcgacgac gacggccgga tcatcaatgt gcccgcgtgc ggccacgccg atgcagcgct
      841 cctcagggtc gaccgggtgg agatccgaat ggacgccggg cgcatctcgt cgtttgccag
      901 ccggggcatc cgcgggccgc agttccttgt cgtggtggcc aacggtttcg attcgttccc
      961 tacgaaccag cgtcctgcgg cgctgccgcc atgcgcgggc catccgaccg tcgtgccgtg
     1021 agcccgacct cgatctagac ttcgaggtga tgaccaccca cgtcgaccgg ccagagacca
     1081 gccctgtcgg cgatggaccg gaaccgactc gttcaccaac gatcggcgag ggcgaccggg
     1141 tcgagctcct cgattatggc ttcgtccgcc tcgatgcctc gatggccgac gacctgtccg
     1201 tcgccaacgc cgctcgcgtc agcttcgccc gccggaagga ggagctcgac gactccgacc
     1261 gagggctcat ccgcttcctc atgcgcgatc gacatggaac gccgttcgag cacaacagtt
     1321 tccggttcca catccgctgc ccgatcttcg tcgccaggga atggatgcgg cataggatcg
     1381 gctgcttgac gggcgagaca gtcgtgacgt tcgtcgatac gaacggccat gcgactccgc
     1441 tgttggcgaa gtcccttgat gcgctctggc agagctgggc cgaaggcgag cgggacgggc
     1501 acgcagttga tgaaggcacg gcatctcgca ttcgcgagtt ggcggcgatg ggagtcagcc
//
LOCUS       DATBSW010000365         8146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_35262, whole genome shotgun sequence.
ACCESSION   DATBSW010000365 DATBSW010000000
VERSION     DATBSW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35262"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VIF63_09955"
     CDS             complement(<1..248)
                     /locus_tag="VIF63_09955"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8239746.1"
                     /translation="MSFIAWIVVGAIAGFIATRVTNASGGLLMTVGLGIVGGLVGGWF
                     AANVLKMGSVDGLNIESIVIATLGAVAVVWIANAASGKG"
     gene            complement(298..702)
                     /locus_tag="VIF63_09960"
     CDS             complement(298..702)
                     /locus_tag="VIF63_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239747.1"
                     /translation="MTTTGAAPSVAPGTTPVSTAHESIQTASRSTTSSPGRPELGRRI
                     AVLVFGLIQLVIGARFVLLLLDAREGNDLVSGILNLSQIFVGPFEGILRTDALHAAGS
                     TFEITAIVALIGWTILEFIVIKALAIFQREAV"
     gene            complement(767..970)
                     /locus_tag="VIF63_09965"
     CDS             complement(767..970)
                     /locus_tag="VIF63_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239748.1"
                     /translation="MAEDPAVSAWESMLIETHGTADAVSRRASLHGPVIFALRTIAFL
                     LIAALAILVLLPAALTAQAAIAV"
     gene            1137..1535
                     /locus_tag="VIF63_09970"
     CDS             1137..1535
                     /locus_tag="VIF63_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8239749.1"
                     /translation="MAPRIGRLLKRTRQVRALSATDVARKAGISAAYLSKLENDAVKK
                     PSPPVLHQLSEALAVPYAELMRLSGYPVPGNEGPSTGDSIGAALFADITEDERDELLE
                     YLAWYRARRRSPRGTSAPTKRVQVPGPIPV"
     gene            1956..3821
                     /gene="ilvB"
                     /locus_tag="VIF63_09975"
     CDS             1956..3821
                     /gene="ilvB"
                     /locus_tag="VIF63_09975"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY8239750.1"
                     /translation="MTLTKGKRARLSHMPADGTEHAAAVERAQREMLHEGTASQAVRT
                     GEHANRPRIGADVLCEGLLRQGVDVMFSYPGGVILPLYDILGDYPQLRHVLVRHEQGG
                     AHAADGYARATGKVGVCVGTSGPGATNLVTGIGTAMLDSIPMVAITGNVPSALIGKDA
                     FQEIDINGITLPMTKHNYLVRDANELPRVIAEAFHIARSGRPGPVHIDITKDALQQQT
                     EAPHPSIEEIVAGLPGFRPTMEGNYKQLKLAAAEIAAAERPVILAGHGILHARAEAEL
                     LAFAEKLNAPVAWTLLGIGAIDERHPLAYGFMGMHGWKHVNRAIQSADLLVALGMRFD
                     DRVTGNVRTYAPYARIIHVDIDPAEIGKNVAVEVPVVGDAKRVLTTLTPMVDAVPAEQ
                     RAPYLEQLAEWKRDSEGSSWHGSGAWRDGLLSADYVVQRIGELTEHEGIYVGDVGQNQ
                     MWLARYAGFRHPNTHLSSGGLGTMGYAVPAAMGAALGVPERETWAIVGDGGFQMTSQE
                     LMTLVQDEIPVKIALFDNKKLGMIRQWQEIIYAGNFHSAQLMGPDYLKLAEAYGIPSM
                     KASTPAEVDEAIKFAQGVDGPALIWFEIAGEQNVFPMMPAGKGLSDLIEEWGAPE"
     gene            3818..4438
                     /gene="ilvN"
                     /locus_tag="VIF63_09980"
     CDS             3818..4438
                     /gene="ilvN"
                     /locus_tag="VIF63_09980"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY8239751.1"
                     /translation="MSAAEPTATQAAMTAPAATTVVPAHTAPPPRAMPGGGQQKLHRL
                     VAIVADRPGVLNRVSSLMRARNFNIDSLAVSRTDREGFSRMTISLHGDDVAVEQAAKQ
                     LYRLIDVLKVQDVTAEPTVEHELALVKVRVVEGNRSEVLKLIELARGRVLDVSTEAII
                     AEVSGSEAEVDDFVACVRGFGIKELVRTGAVVMARGAGSIEEAVKR"
     gene            4452..5561
                     /gene="ilvC"
                     /locus_tag="VIF63_09985"
     CDS             4452..5561
                     /gene="ilvC"
                     /locus_tag="VIF63_09985"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY8239752.1"
                     /translation="MTARMYYDNDVEPGALEGQMVAVIGYGSQGHAHALNLRDSGVDV
                     IVGLAEGSKSRALAEAAQLRVATVAEAVRDADVVMILVPDTAQKAVYDKDIEPNLKPG
                     TLLMFAHGFNIRFARIKPGPGIDVGMVAPKGPGHLLRSVYSAGGGVPALFAVEQDASG
                     TARARTLAYARALGSTRAGILETTFKEETETDLFGEQALLCGGVSALIKAAFETLVEA
                     GYQPELAYFETMHELKLIVDLMYRGGLNFMRFSVSDTAEFGDYVSGPRVAEGVKATMK
                     DVLADIQSGSFANRWIAVQEAGGGEFRELRKRDQDHQIEKVGADLRAQMAFLDPVVVH
                     AGEAQATVAGAASVEAGAGGAATATQPASDSHSTF"
     gene            5630..7225
                     /locus_tag="VIF63_09990"
     CDS             5630..7225
                     /locus_tag="VIF63_09990"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8239753.1"
                     /translation="MRVFDTTLRDGEQAPGAGLTAAEKLEVARQLVRLKVDVIEAGFP
                     AASEGDFQAVQRIARETKGGIAVAALARCRDGDPQRAIEAIKGAERPHLHVFIATSDI
                     HLKHKLRIDRETALREAVKWVAYGREQLGRDVEIEFSAEDASRTDPEYLMQVYEAVVE
                     AGASTINIPDTVGYAIPSEFAALVARTVGLLGSQATISVHCHNDLGLATANTLAAVQA
                     GARQVEVTINGLGERAGNASLEEVVMALRTRPTQFQSTGSALTHGVQTEHITAASRLV
                     SYLTGFAIQPNKAVVGGNAFAHESGIHQDGVIKNPLTYEIMTPQSVGLSSNSITIGKL
                     SGRKGISTKLAELGYELEGEALDAIYRQAIELADEKKEVTDDDLIALVEQRVAEVPQA
                     VRLEGWNVTSSHGGKATGMVALSVGDEDHAKAAIGNGPVDALFEAVDAALEPVFGWHP
                     VLETYEIKAVSGGEDAQGRVLVRCRRSSDEGPGALIVSGHGLSTNIIEASIEAYLVAV
                     NKLHGAQGQASSAFVGARTTELP"
     gene            7230..>8146
                     /gene="leuB"
                     /locus_tag="VIF63_09995"
     CDS             7230..>8146
                     /gene="leuB"
                     /locus_tag="VIF63_09995"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY8239754.1"
                     /translation="MNAYRIASIGGDGVGPEVIAAARRVLDAVATRDGFTVEWLDVVA
                     GGAAIDAYGVAIRTEDLDAVAACDAVLLGAVGGPRWDDPDARVRPEQALFALRGGLEL
                     FANLRPVTILPALVPSSPLRPEILEGVDLLIVRELTSGLYFGRPSEQRDTPEGRVGID
                     TLWYTEGEIRRVVRLAFELAGGRRGRLTSVDKANVLATSRLWRKVVDETRPEFPGVEV
                     NHQLVDSCAMLLIRKPAQFDVLVTENLFGDILSDEAAVLAGSLGMLPSASVGERRTAH
                     GRFGLYEPIHGSAPDIAGKDLANPLGTVLS"
BASE COUNT         1325 a         2809 c         2845 g         1167 t
ORIGIN      
        1 cccttgcccg atgcggcatt cgcgatccag acgaccgcga ccgccccgag cgtggcaatg
       61 acgatgctct cgatgttcag gccgtcgacg ctgcccatct tgagcacgtt ggctgcgaac
      121 cagccgccga cgaggccgcc gacgatgccg aggccgacgg tcatcagcag accgccgctg
      181 gcattcgtga cccgggtggc gatgaagccg gcgatggcgc cgacgacgat ccaggcgatg
      241 aaactcatca gatcagttcc tttcgggagg tatacgtcgt gcgtggccag cgtcggatca
      301 gacggcctcg cgttggaaga tggcgagtgc cttgatcacg atgaattcga ggatcgtcca
      361 gccgatgagg gccacgatcg cggtgatctc gaaggtggag cccgccgcgt ggagtgcgtc
      421 ggttcgaagg atgccttcga acggaccgac gaagatctgg ctcaggttga ggattccgga
      481 gacgaggtcg ttgccttcac gcgcgtccag caggagcagg acgaaccgtg ccccgatgac
      541 gagctggatg agcccgaaca cgagcacggc gatgcgccgg cccagctcgg gacggccggg
      601 gctcgaggtg gtgctgcggc tggcggtctg gatcgactcg tgggcggtgc tgaccggcgt
      661 ggtgcccggt gcgaccgaag gtgcggcccc ggtcgtggtc acggttcgtt cgatgctctc
      721 ttgtgtcatt gcttctccag ttgggctgtc ggtgatgcga agcgacctag accgcgattg
      781 cggcctgggc tgtgagcgct gccggaagca gcacgaggat cgccagcgcg gcgatgagga
      841 ggaacgcgat cgtgcgcagc gcgaagatca cgggtccatg gagggaagcg cggcggctga
      901 ccgcgtctgc ggtgccgtgc gtctcgatga gcatggattc ccatgcgctg acagccgggt
      961 cttcggccac gcctgcctcc aggtgcccaa tcaggcatcc ggggaagcgg ggccaaaagg
     1021 gcctccgccg gcttgtgctc cggccactgc tgccacttga gtacaacagg tgaccgtgct
     1081 attgtcaact gtagtttcat atatcaactg taggtacgag ggcacggagg acagcgatgg
     1141 caccacggat cgggcggctg ttgaagcgca ctcgacaggt gcgcgcgctc tccgcgacgg
     1201 acgtcgcgcg caaggcaggg atctcggccg cctacctcag caagctcgag aacgacgcag
     1261 tcaagaagcc gtcgccgccg gtgctccacc agctgagcga ggcgctcgcc gtgccctatg
     1321 cggagctcat gcgcttgagc ggctacccgg tgccgggcaa tgaaggaccg tcgacgggcg
     1381 attcgatcgg ggccgcgctg ttcgcggaca tcaccgagga cgaacgcgac gagctgctcg
     1441 aatacctggc ctggtaccgc gctcggcgcc ggtcgccgcg cggcacgtcc gccccaacga
     1501 agcgcgtgca ggtgccgggg ccgatcccgg tctgagccgc gcggagccgt cctcccccga
     1561 ccagtactgc ccgcccagat cgccaccgat cggtaccggt tgcgcaatca acgtgcggcc
     1621 ccgtagactg cgcgcaccaa tggccttctc gaatgagcct gatcgccgcc ccgtcgtcat
     1681 cgccgtgacg actgacggtc gccgcgtcca gatgcccgtc gaggacgtcg aggtcccctt
     1741 cctctccctt attcgctcga tcctggtcgg gattgaggag ctcgtactcc tcattacgaa
     1801 ccggcgggag cacggaggga cgcggcggga cgatcgctag gcgatcagca ccaccagaac
     1861 ccaacggacc cccgccgaga ggcgggggtt tttgattgac cggcaggttc gccggtgaac
     1921 ggaggcatcg tgaccgaaac gcgagggcag caaccgtgac gctgacgaag ggcaagcgcg
     1981 cgcgcctgag ccacatgccg gccgatggca ccgagcacgc ggccgcggtc gagcgcgccc
     2041 agcgcgaaat gctccacgaa ggcacggcca gccaggcggt gcgcacgggc gagcacgcga
     2101 atcggccgcg gatcggcgcg gacgtgctgt gcgagggcct gctccggcag ggcgtcgacg
     2161 tcatgttcag ctaccccggc ggcgtgatcc tgccgctgta cgacattctc ggcgactacc
     2221 cgcagctgcg ccacgtgctc gtgcgccacg agcagggcgg cgcccacgcc gccgatggct
     2281 acgcccgggc gacgggcaag gtcggcgtgt gcgtgggcac ctccggcccg ggcgcgacga
     2341 acctcgtgac cggcatcggc accgcgatgc tcgactcgat cccgatggtc gcgatcaccg
     2401 gcaacgtgcc ctccgccctc atcggcaagg acgcgttcca ggagatcgac atcaacggca
     2461 taaccctgcc gatgaccaag cacaactacc tcgtgcgcga cgcgaacgag ctgccccggg
     2521 tcatcgccga agcgttccac atcgcccgca gcggccggcc cgggccggtc cacatcgaca
     2581 tcaccaagga cgcgctccag cagcagaccg aggcgcccca cccgtcgatc gaggagatcg
     2641 tcgccggcct gcccggcttc cgcccgacga tggagggcaa ctacaagcag ctgaagctcg
     2701 cggccgccga gatcgccgcg gccgagcggc cggtgatcct tgccggccac ggcatcctcc
     2761 acgcccgcgc cgaggcagag ctcctggcct ttgccgagaa gctgaacgct ccggtcgcgt
     2821 ggaccctcct gggcatcggc gcgatcgacg agcggcaccc gctcgcgtac ggcttcatgg
     2881 gcatgcacgg ctggaagcac gtgaaccggg cgatccagtc ggccgacctg ctcgtcgcgc
     2941 tcggcatgcg cttcgacgac cgggtgaccg gcaacgtccg cacgtatgcc ccgtatgcgc
     3001 gaatcatcca tgtcgacatc gacccggcgg agatcggcaa gaacgtcgcg gtcgaggtcc
     3061 cggtcgtcgg cgacgcgaag cgcgtgctga ccacgctcac accgatggtc gacgcggtgc
     3121 cagcggagca gcgtgcgccg tacctcgagc agctggccga gtggaagcga gattcggaag
     3181 ggagctcgtg gcacggatcc ggcgcctggc gcgacggcct gctgtcggcg gactacgtgg
     3241 tccagcggat cggcgagctg accgagcacg agggcatcta cgtcggcgac gtcgggcaga
     3301 accagatgtg gctcgcgcgc tacgccggct tccgccaccc gaacacgcac ctgtcctcgg
     3361 gcggcctcgg gacgatgggc tacgcggtgc ccgccgccat gggcgccgcc ctgggcgtgc
     3421 ccgagcggga gacctgggcg atcgtcggcg acggcggctt ccagatgacg tcgcaggagc
     3481 tcatgaccct cgtccaggac gagatcccgg tgaagatcgc gctcttcgac aacaagaagc
     3541 tcgggatgat ccgccagtgg caggagatca tctacgcggg caacttccac tcggcccagc
     3601 tcatgggccc cgactacctg aagctcgccg aggcgtacgg catcccgtcg atgaaggcgt
     3661 cgacgccggc cgaggtcgac gaagcgatca agttcgccca gggcgtcgac ggtccggcgc
     3721 tcatctggtt cgagatcgcg ggcgagcaga acgtcttccc gatgatgccg gcgggcaagg
     3781 gcctgtccga cctgatcgag gagtgggggg cgcccgaatg agtgccgccg agcccacggc
     3841 gacgcaggcg gcgatgaccg cgccggccgc gaccacggtc gtgccggcgc acaccgcccc
     3901 gcccccgcgg gcgatgcccg gcggcggcca gcagaagctg catcggctcg tggccatcgt
     3961 ggccgaccgg ccgggtgtcc tgaaccgcgt gtccagcctc atgcgcgccc gcaacttcaa
     4021 catcgactcg ctcgccgtca gccgcaccga tcgcgagggc ttctcgcgaa tgaccatcag
     4081 cctccacggc gacgacgtcg cggtcgagca ggccgcgaag cagctctacc ggctgatcga
     4141 cgtgctcaag gtccaggacg tgaccgccga gccgacggtc gagcacgagc tcgcgctggt
     4201 caaggtgcgc gtcgtcgagg gcaatcggag cgaggtgctc aagctgatcg agctggcgcg
     4261 cgggcgcgtg ctggacgtct cgaccgaggc gatcatcgcc gaggtcagcg gcagcgaggc
     4321 ggaggtcgac gacttcgtcg cctgcgtccg tggcttcggg atcaaggagc tggttcgcac
     4381 cggggccgtc gtgatggccc gcggcgcggg atcgatcgag gaggcagtca agcgatgagg
     4441 gaacacgacc gatgacggca cgcatgtact acgacaacga cgtcgagcct ggggcgctcg
     4501 aaggccagat ggtcgccgtc atcggctacg gcagccaggg ccacgcgcac gccctgaacc
     4561 tgcgcgactc cggggtcgac gtcatcgtcg gcctggcgga gggatcgaag agccgcgcgc
     4621 tcgccgaggc cgcccagctt cgcgtcgcca ccgttgccga ggctgttcgc gacgccgacg
     4681 tcgtgatgat cctcgtgccg gacacggccc agaaggcggt ctacgacaag gacatcgaac
     4741 cgaatctcaa gcccggcacc ctgctcatgt tcgcgcacgg cttcaacatc cggttcgcgc
     4801 ggatcaagcc gggcccgggg atcgatgtcg ggatggtcgc gccgaaaggc cccggccacc
     4861 tcctgcgcag cgtgtactcg gccggcggcg gggtcccggc cctgttcgcc gtcgaacagg
     4921 acgcatcggg caccgcccgt gctcgaacgc tcgcctacgc gcgggcactc ggctcgaccc
     4981 gcgcggggat cctggagacg acgttcaagg aggagaccga gaccgatctg ttcggcgagc
     5041 aggccctcct ctgcggcggc gtgtcggccc tgatcaaggc cgcgttcgag accctcgtcg
     5101 aagccggcta ccagcccgag ctcgcctatt tcgagacgat gcacgagctg aagctgatcg
     5161 tcgacctcat gtaccgcggc ggcctcaact tcatgcgctt cagcgtcagc gacacggcgg
     5221 agttcggcga ctacgtctcc ggcccgcgcg tcgcggaggg cgtgaaggcc acgatgaagg
     5281 acgtcctcgc cgacatccag agcggctcgt tcgcgaatcg ctggatcgcc gtgcaggaag
     5341 ccggcggcgg cgagttccgg gaactgcgaa agcgcgacca ggaccaccag atcgaaaagg
     5401 tgggcgccga cctgcgggcc cagatggcct tcctcgatcc ggttgtcgtc cacgccggcg
     5461 aggcgcaggc gaccgtggca ggcgccgcgt cggtggaagc cggcgccggt ggcgccgcca
     5521 cggcgaccca gccggcctcg gactcccact ccacgttctg atggccgctt ccgcccgacc
     5581 tcgatcccgg cctgctcgcc catccggccc gctcgttccg gccggcagcg tgcgcgtctt
     5641 cgacacgacc ctgcgtgacg gcgagcaggc gcccggcgcg ggcctcaccg cggcggagaa
     5701 gctcgaggtc gcgcgccagc tcgtccggct caaggtcgac gtcatcgagg ccggcttccc
     5761 ggccgcgtcc gagggcgact tccaggccgt gcagcggatc gcgcgggaga cgaagggcgg
     5821 gatcgccgtt gcggccctcg cccgctgccg cgacggcgac ccgcagcgcg cgatcgaggc
     5881 gatcaagggc gccgagcgac cacacctgca cgtgttcatc gcgaccagcg acatccacct
     5941 gaagcacaag ctgcgcatcg atcgcgagac ggcgctgcgc gaggcggtga agtgggtcgc
     6001 ctacgggcgc gagcagctcg ggcgggacgt cgagatcgag ttcagcgccg aggacgcctc
     6061 gcgcaccgat cccgagtacc tcatgcaggt ctatgaggcc gtggtcgagg ccggcgcgtc
     6121 gacgatcaac atcccagaca cggtcggcta tgccatcccg tcggagttcg cggcgctggt
     6181 cgcccggacc gtgggcctcc ttggcagcca ggccacgatt tccgtccact gccacaacga
     6241 cctcgggctg gccacggcga acaccctcgc cgccgtgcag gccggcgcgc gccaggtcga
     6301 ggtgacgatc aacggcctcg gcgagcgcgc gggcaatgcg tcgctcgaag aggtcgtgat
     6361 ggcccttcgg acccgcccca cgcagttcca gtcgacgggc agcgcgctca cgcacggggt
     6421 ccagaccgag cacatcaccg cggcgtcgcg gctcgtgagc tacctcaccg ggttcgcgat
     6481 ccagccgaac aaggcggtcg tcgggggcaa cgcgttcgcc catgagtcgg gcatccacca
     6541 ggacggcgtg atcaagaacc cgctgacgta cgagatcatg accccgcagt cggtcgggct
     6601 gtcgagcaac tcgatcacga tcggcaagct ctccggaagg aagggcatct cgaccaagct
     6661 cgcggagctc ggctacgagc tcgagggcga ggccctcgac gcgatctacc gccaggcgat
     6721 cgagctcgcc gacgagaaga aggaggtcac cgacgacgac ctcattgccc tcgtcgagca
     6781 gcgcgtcgcg gaggtcccgc aggccgtgcg gctcgaaggc tggaacgtga cgtcgtccca
     6841 tggcggcaag gcgacgggca tggtcgcgct gtcggtcggc gacgaggacc acgccaaggc
     6901 cgcgatcggc aacgggccgg tcgatgccct gttcgaggcc gtcgatgcgg ccctcgagcc
     6961 ggtgttcggc tggcatcccg tgctcgagac ctatgagatc aaggcagtct ctggtggcga
     7021 ggacgcccag ggccgcgtgc tcgtgcgctg ccgccgctcc agcgacgaag gcccgggcgc
     7081 gttgatcgtg tccggccacg ggttaagcac gaacatcatc gaggcctcga tcgaggcgta
     7141 cctcgtggca gtcaacaagc tccacggcgc acagggccag gcgtcctccg cgttcgtcgg
     7201 cgcccgcacg accgagctgc cgtagccgcg tgaacgcgta ccgaatcgcg tcgatcgggg
     7261 gcgatggcgt cggcccggag gtgatcgcgg ctgcccgccg cgtcctcgac gcggtcgcga
     7321 cccgcgacgg gttcactgtc gaatggctcg atgtcgtggc cggcggggcg gcgatcgatg
     7381 cctacggcgt ggcgattcgc accgaggacc tggacgcggt cgcggcgtgc gacgcggtgc
     7441 tcctcggcgc ggtcggtggg ccgcgctggg acgacccgga tgcgcgcgtc cggccggagc
     7501 aggcgctgtt cgcgctccgt ggcggcctcg agctgttcgc caacctgcgc ccggtcacga
     7561 tcctgcccgc gctcgtgccg tcgtcgccgc tccggcccga gatcctcgag ggcgtcgacc
     7621 tgctgatcgt ccgcgagctc acctcggggc tgtacttcgg tcgcccctcg gagcagcgcg
     7681 acacgcccga gggtcgcgtc ggcatcgaca cgctctggta cacggaaggg gagatccggc
     7741 gcgtcgtccg gctcgcgttc gagctcgccg gaggccgccg cggccgcctc acgagcgtcg
     7801 acaaggccaa tgtccttgcg acgtcgcgac tgtggcgcaa ggtcgtcgac gagacgcggc
     7861 cggagttccc gggtgtcgag gtcaaccacc agctcgtcga ctcgtgcgcc atgctcctga
     7921 tccggaagcc cgcccagttc gacgtcctcg tgaccgagaa cctcttcggc gacatcctct
     7981 cggacgaggc cgccgtgctc gcaggcagcc tcggcatgct cccgtcggcg tcggtcgggg
     8041 agcggcggac cgcgcacggg cgattcggcc tgtacgagcc aatccacggc tccgcgccgg
     8101 acatcgccgg caaggacctg gcgaatccgc tcggaacggt cctctc
//
LOCUS       DATBSW010000366         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_951707, whole genome shotgun sequence.
ACCESSION   DATBSW010000366 DATBSW010000000
VERSION     DATBSW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951707"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1143
                     /locus_tag="VIF63_10000"
     CDS             127..1143
                     /locus_tag="VIF63_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239755.1"
                     /translation="MLLAVAVVLAACGGSSASPVATQVTSTGSTSAAPTAVVSTPNPT
                     ATPPYAPVARTSAAITWDPTSQAVVLFGGDSRFGATSEFDAWDGATWSKLVEIGPPSR
                     DDGLLVADPERGVVVLAGGRNQQIVQDDTWEWDGTTWSEKDVVGPPPRAHAAAAYDHE
                     SKRMIVYGGVTETETMRDTWAWDGTAWTKLDDKGIPGLIPNGMAWDPILERLLVLAVD
                     LEAESSDHTYPSELWGWTGDGWERVAKDGPSFSPLQQFVEGPRHPWLIDGGVAQGAFS
                     TLEWTGTAWQPLDGPAPPLRNGQAVAFDQVRHQMVLFGGFVDDTVLGDSWLLDDGAWR
                     EVGA"
     gene            1170..1598
                     /locus_tag="VIF63_10005"
     CDS             1170..1598
                     /locus_tag="VIF63_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8239756.1"
                     /translation="MDWTLEVVIVPVSDIDRAVAFYRDQVGFHLDHDTKNEHMHVVQL
                     TPNGSGCSIVIGDLPSQNEMEPGSLRGLQLVVADGRAARRELIDRGVECSEIMVFDER
                     DGGTFFGFSDPDGNSWAVQELKVRGESPLIPLEHRSRFGA"
     gene            1745..2296
                     /locus_tag="VIF63_10010"
     CDS             1745..2296
                     /locus_tag="VIF63_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8239757.1"
                     /translation="MDRDLVEAARKRDMAAYADLIRIRGDRLYALAHRILRDVDRAED
                     AVQEALVIAWRDLPGLRDPDRFDAWLHRLVVRSCLAEAKRERRRITNLHVLPLDLPMA
                     GDDFLNVDDRDQLDRGFRRLSPEQRALLVLRHFEGRELTDIAEVLGIPVGTVGSRLHT
                     AHRAMRAALDANARVIAIEGRPA"
     gene            2293..3504
                     /locus_tag="VIF63_10015"
     CDS             2293..3504
                     /locus_tag="VIF63_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239758.1"
                     /translation="MNRNDDIERVLEQWFTEGPRHMPDRLFDGTLERIERLPRGRLAD
                     LQSRLPAMNLNVRLAAAAALIVVLAGASFVLSRAPDVGTSPSPTPAALADAIQAQWTS
                     VGDRPCFGISECHSPYSILVDATTLHVGEFKGDIVSTWSLGPAGELLVSAPQSLVVNK
                     GWHECGYGAEGSYRVSVSPDAATLTLTLANDSCQNRAGILPGEWTRSECTWGWLLCFP
                     ERHAGPAPHVASVFRPFGPASSARFAYTIAASILAESDANIQFGNPYAPEGMGILVVA
                     QATLDASGCGDDPGQAEMTPTGIAAWLASFPTLVVTTPVPIRIGGLSGVMVDVSRNTD
                     ITVLCGTNFGVWTIGSALMKVHADDKIRVILLERPDDVTLVITSTGRGFPDWHPVTDA
                     MPIIEGFEFQP"
     gene            complement(3553..3942)
                     /locus_tag="VIF63_10020"
     CDS             complement(3553..3942)
                     /locus_tag="VIF63_10020"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEY8239759.1"
                     /translation="MPRDAYEYAILRVAPRVERGELVNVGVVVFCRTRRYLGMRADLG
                     ERQTAALQALVPDVDLEVVRAHLASIQSIVEGETSAGPIAQLAAPERFRWVTSPSSTV
                     IQPSDVHGGMTDDPAASLEELYEKLAR"
     gene            complement(3932..4732)
                     /locus_tag="VIF63_10025"
     CDS             complement(3932..4732)
                     /locus_tag="VIF63_10025"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY8239760.1"
                     /translation="MSSPTLRTVTATKYLTPFREGGSLPGLVEADDDGLYVVKFRGAA
                     QGPRALVAEVIAGELGRALGLPVPELVLVDVDPALAGVEPDPWVGELLSKSPGMNLGI
                     DYLPGSMTFDPRADGAPPADLAADIVWFDALVTNTDRSPRNPNILSWHHRLWLIDHGA
                     SLFIHHTWLEPDAHARRPLATIAEHVLLPYASSIEAAHERLAPRITREVLDPVVALVP
                     DAWLEPIGAAAVIGGPAEQRAAYVRYLLTRVAARDALVDSIEGVRHAA"
     gene            4811..>5062
                     /locus_tag="VIF63_10030"
     CDS             4811..>5062
                     /locus_tag="VIF63_10030"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8239761.1"
                     /translation="MTTDANSWEDQLIADLRANGGRPSQGPLAGETLLILWTTGAKSG
                     QRRRSIVNYHMEGDDYYVAGTKGGAPTNPAWIANIAANPD"
BASE COUNT          813 a         1747 c         1731 g          771 t
ORIGIN      
        1 cagtcgcggc ggcagcgcgc cggctctgag cggggccacg gcgccaccac gccgtttcac
       61 cccttgctgt caccatgcgg ggcagtcgcg gctcatcccg atatggccca caagcgatgg
      121 cgtacgttgc tccttgcggt cgccgtcgtc ctcgccgcgt gtggcgggtc gagcgcatca
      181 cccgtcgcga cacaggtgac cagcaccggg tcgacctcgg ccgcgccgac cgccgtcgtc
      241 tcgaccccga acccaaccgc aacgccgccc tacgcgcccg tggctcgcac ctccgctgcg
      301 atcacctggg acccgacgag ccaggccgtc gtgctcttcg gcggcgattc gcgcttcggt
      361 gcgacgagcg agttcgatgc ctgggacggc gcgacctggt cgaagctggt cgaaatcggc
      421 ccgccgtcgc gcgacgacgg actcctcgtt gcggatccgg agcgcggcgt tgtggtgctc
      481 gccgggggtc gcaatcaaca gatcgtccag gacgacacgt gggagtggga cgggacgacc
      541 tggtcggaga aggacgtggt cgggccgccg ccacgcgcgc acgccgctgc tgcgtacgac
      601 cacgaatcga agcgaatgat cgtgtacggc ggcgtcaccg agaccgagac catgcgcgac
      661 acgtgggcgt gggacggcac ggcctggacg aagctcgatg acaagggcat ccccggcctg
      721 atcccgaacg gcatggcctg ggacccgatc ctcgaacggc tgctggtgct cgcggtcgac
      781 ctcgaggccg agagctcgga ccacacctac ccgtcggagc tgtggggctg gaccggtgac
      841 ggctgggaac gcgtcgcgaa ggacgggccg tcgttcagcc cgctccagca attcgtggaa
      901 gggccgcgcc acccgtggct catcgatggc ggggtcgcgc agggcgcctt ctcgacgctt
      961 gagtggaccg ggacggcgtg gcagccgctc gacggccctg cgccgccgct ccggaacggc
     1021 caggcggtcg cgttcgacca ggtccgccac cagatggtgc tgttcggcgg gttcgtcgac
     1081 gacacggtcc tcggcgactc ttggctgctc gacgatggcg cctggcgcga ggtcggcgcc
     1141 taactgccat taactgccat catcggcgga tggactggac cctcgaagtc gtcatcgtgc
     1201 ccgtcagtga catcgatcgc gccgtcgcgt tctaccgcga ccaggtcggt ttccacctcg
     1261 accacgacac gaagaacgag cacatgcacg tcgtgcagct gaccccgaac ggctcgggct
     1321 gctcgatcgt catcggcgac ctgccgtcgc agaacgagat ggagccgggc tccctgcgcg
     1381 gcctccagct cgtcgtcgcg gacggccgcg cggcccgccg ggagctgatc gatcgcggcg
     1441 tggaatgcag cgagatcatg gttttcgacg agcgcgacgg cggcacgttc ttcggcttca
     1501 gcgaccccga cggaaactcg tgggccgtgc aggagctcaa ggtccgcggc gagtcgccgc
     1561 tgattccgct cgagcatcgg agccgcttcg gcgcctgatc ctcgatcggg cccggagacc
     1621 gggcgtagga tgggttcgtc cggtcggacg actgctccat tcacgaagaa tgaatgcccc
     1681 tcggcgactt gatcccatag ggactggtca gcgcctccat ttggcgcccc agggatcgga
     1741 gccgatggac cgggacctcg tcgaggctgc acggaagcgg gacatggcgg cgtatgccga
     1801 cttgatccgc attcgtggcg accgcctcta cgcgctcgcc caccggatcc tccgtgacgt
     1861 cgaccgggcc gaggatgccg ttcaggaagc gctggtgatc gcctggcgtg acctgcccgg
     1921 acttcgcgat ccggatcgct tcgacgcctg gctccatcgg ctggtcgttc gctcgtgtct
     1981 cgccgaggcg aagcgcgaac gccgccggat cacgaacctg cacgtgctcc cgctcgacct
     2041 cccgatggcc ggcgacgact ttctcaacgt cgacgatcgg gatcagctcg accggggctt
     2101 ccgacgcctt tcccccgagc agcgcgcgct actcgtcctc cgccatttcg agggtcgtga
     2161 gctgaccgac atcgccgagg tgcttggcat cccggtgggg accgtcggtt cacgcctcca
     2221 caccgcccac cgcgcgatgc gcgctgcgct ggacgccaac gcccgagtga tcgccatcga
     2281 aggacgaccg gcatgaaccg aaacgacgac atcgaacgcg tcctcgagca gtggttcacc
     2341 gaaggaccac ggcacatgcc ggaccggctc ttcgacggca ccctcgagcg catcgaacgg
     2401 cttccacgcg gccgcctcgc ggacctgcag tcgaggttgc ctgccatgaa cctgaacgtc
     2461 cgcctcgcgg ccgcagccgc gctcatcgtg gtcctcgccg gggccagctt cgtgctcagc
     2521 cgagcgcccg acgtcggaac gagtccatcg ccgacgccgg cggcgctcgc cgatgcgatc
     2581 caggcgcaat ggacgtcagt cggcgatcgt ccctgcttcg ggatcagcga atgccattcc
     2641 ccctattcca tcctcgtcga tgcgaccacg cttcacgtcg gcgagttcaa gggcgacatt
     2701 gtcagcacct ggtcgctagg tccggcaggg gaactcctgg taagcgcgcc gcagtcgctc
     2761 gtggtgaaca agggctggca cgaatgcggc tatggcgccg aagggagcta tcgggtcagc
     2821 gtctctccgg atgccgcgac gctcaccctg acgctagcga acgactcgtg ccagaatcgg
     2881 gccggaatcc tgcccggcga atggacacgc tcggaatgca catggggctg gctgttgtgt
     2941 ttcccggagc gccatgccgg accggcaccg cacgtcgcct cggtcttcag accgttcggc
     3001 ccggccagct ccgctcggtt cgcgtacacg attgcggcgt cgatcctggc cgagagtgac
     3061 gcgaacatcc agttcgggaa tccctacgct cctgagggga tggggatcct cgtggttgcg
     3121 caggcaacgc tggacgcctc cgggtgcggc gatgatccag ggcaggccga gatgactcca
     3181 accgggatcg cggcgtggct tgccagcttc ccgacactcg tcgtgacaac tccggtgccg
     3241 atcaggatcg gcgggctgtc tggggtgatg gtcgacgtgt cgcgaaacac ggacatcacc
     3301 gtgctctgtg gcaccaactt cggtgtctgg accatcggct ccgcgctcat gaaggttcat
     3361 gcagacgaca agattcgcgt catcctcctc gaacgccccg acgatgtgac gctcgtgatc
     3421 accagcacag gacgtgggtt tccggactgg catccggtga cggatgcaat gccgatcatc
     3481 gaagggttcg agttccagcc ctgaaagcga ccatccaggt ccgtcgtgag ggccgcgacg
     3541 cgacctgcgc aactagcgcg cgagcttctc gtagagctcc tcgagcgagg cggccgggtc
     3601 gtcggtcatg ccgccgtgga cgtcggacgg ctgaatgacc gtgctcgacg gcgacgtgac
     3661 ccagcggaag cgctccggcg cggcgagctg ggcgatcggc ccggcgctcg tttcaccttc
     3721 gacgatcgac tggatcgagg cgagatgcgc gcggacgact tcgagatcga cgtcggggac
     3781 cagtgcctgc agggcggcgg tctgtcgttc gccgaggtcg gcgcgcatcc cgagatagcg
     3841 gcgcgttcga cagaacacga cgacgccgac gttcaccagc tcaccgcgct cgacgcgtgg
     3901 cgcgacgcgc aggatcgcgt actcgtaggc gtcacgcggc atggcgcacg ccctcgatcg
     3961 aatccacgag cgcgtcgcgg gcggccaccc gcgtgagcag gtagcgcacg taggcggcgc
     4021 gctgctcggc cgggccgccg atcaccgccg cggccccgat cggctccagc cacgcgtccg
     4081 ggacgagcgc gaccacgggg tcgaggacct cccgcgtgat gcgcggcgca agccgctcgt
     4141 gcgcggcctc gatcgacgag gcatacggca gcaggacgtg ttcggcgatg gtcgcgagcg
     4201 ggcgccgcgc gtgggcgtcc ggttcgagcc acgtgtggtg gatgaacagc gacgccccgt
     4261 ggtcgatcag ccagaggcgg tggtgccacg acaggatgtt cgggttgcgg ggcgaccggt
     4321 cggtgttcgt caccagcgca tcgaaccaga cgatgtccgc ggcgaggtcc gccggcgggg
     4381 cgccgtcggc gcgggggtcg aacgtcatgg agccaggcag gtagtcgatc ccgaggttca
     4441 tgcccgggct cttcgagagg agctcgccga cccacggatc gggctcgacg ccggccagtg
     4501 ccggatcgac atcgaccagg accagctccg gcaccggcag gcccagggcg cgcccgagct
     4561 cgcccgcgat cacctcggcc acgagtgccc ggggaccctg cgccgcaccg cggaacttga
     4621 cgacgtacag gccgtcgtca tccgcctcga cgagccccgg cagcgacccg ccctcgcgga
     4681 agggcgtcag gtacttcgtc gcggtgaccg tgcgcagggt gggggagctc atggccggca
     4741 aagcgtaagc gcattcatgc ccaaggcggt atcgttgcgc acgcaacgac caccgcccag
     4801 aggacccccc atgaccaccg acgccaactc ctgggaagac cagctcatcg ccgatctgcg
     4861 cgccaacggc ggccgcccca gccagggccc gcttgccggg gagacgctcc tgatcctgtg
     4921 gacgaccggg gccaagagcg ggcagcgtcg gcgatcgatc gtgaactacc acatggaagg
     4981 tgacgactac tacgtggccg gcaccaaggg cggcgcgccg accaacccgg catggattgc
     5041 gaacatcgcg gccaacccgg ac
//
LOCUS       DATBSW010000367         7465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_387698, whole genome shotgun sequence.
ACCESSION   DATBSW010000367 DATBSW010000000
VERSION     DATBSW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387698"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..795
                     /locus_tag="VIF63_10035"
     CDS             76..795
                     /locus_tag="VIF63_10035"
                     /inference="COORDINATES: protein motif:HMM:NF036876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HEY8239762.1"
                     /translation="MSAGPQQPGDLPDLQLPEPGTRLGAVSVRVSAALDLAELITTLR
                     DAGAEIVAINTFPATKGEPDVARVFDIETRNFDSAAAARVLRKVPGVIKAEARKTLGQ
                     IFGKRVIVIGGGAQVAQVNLGAVTEADRHNLRGERISVDTIALVGEKQIADAVRAVAD
                     LPRAHILVLAGSIMGGEITKAVRELREVGIPVICLNMAGSVPSAVDLVVSDPVQAGTM
                     AVMAIADTARFSLARQRGRRY"
     gene            825..2102
                     /locus_tag="VIF63_10040"
     CDS             825..2102
                     /locus_tag="VIF63_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8239763.1"
                     /translation="MDARETGRRYLSPVMARYFERDWSHGEGHRLFDVDGRSYLDFAT
                     GIATTILGHRHPRVSAAVHAQVDRLSHVMNGMGYAEPVSRLAERLVATLPAPLDSVFF
                     GNSGAEAIEGALKLARRATGRPAIVAFEGGFHGRTYGALSVTSSNPNYRTGHGPLLPD
                     IHLLPYPLAYREHGGDEAAATAAALGALDAFLTTDVEPRTVAAFLIEPELGEGGYVPA
                     PIEFLQGLRARADQHGILLIADEVQTGYGRTGRMWGFEHAGIVPDVVCAAKGIANGLP
                     LSAIVARRELHERWGKGAHGSTFGGNPVSCAAGIAVLETIEAEGLVASAAERGEQLRG
                     GLQALAAADARIGDVRGRGLMLGVELVRDPATLEPDGALADALIAGCADAGLLLLTCG
                     SAHNIVRWLAPLDVNAAEIDEGLGIFARVLAAA"
     gene            2134..2637
                     /locus_tag="VIF63_10045"
     CDS             2134..2637
                     /locus_tag="VIF63_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8239764.1"
                     /translation="MGALESQAARLEAAAAAIVAMRPNVEAGAPWPLAEVFGPEPEAS
                     WGPPELLAHVEEYLRYWMGEIERVLAADPGTPAPFGRVATDTIRLGVIGRDRTLPFRE
                     LFGRIEADAARVAKRLRELSDDDASRRGVHPARGEMAVRELLEPFLVEHSEGHVKQLR
                     EILATSR"
     gene            2652..3188
                     /locus_tag="VIF63_10050"
     CDS             2652..3188
                     /locus_tag="VIF63_10050"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HEY8239765.1"
                     /translation="MLVAIPVGIVTGWVLRGNIDGLAGLRLRWAWLAVAGLAVQAVLF
                     TERGMDLAGNAVPPIYVVSTLAVFAMVARNIRVTGMPVVALGSLSNLAAIVANGGVMP
                     ADPDAIVAAGLQGDYANSVALPDPALRPLTDIFAIPAGVPLANVFSVGDVLIALGIVI
                     VIAAAMRRSRSTAASTAA"
     gene            complement(3193..4059)
                     /gene="proC"
                     /locus_tag="VIF63_10055"
     CDS             complement(3193..4059)
                     /gene="proC"
                     /locus_tag="VIF63_10055"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY8239766.1"
                     /translation="MSSLAGRTIATVGSGVMAEAIIAGLLRGQLVAPDQIVASHPRGD
                     RRDALTRTHGVRTVDSNAAAVDGADVVLLAIKPQMLARVGREIRGRLQPGQLVISILA
                     GATTQALIGHLGHREVVRSMPNTPAQLGRGMTVWYATPETSAAQREQAGSMLSALGAQ
                     IEVDDEKLVAMATAVSGTGPTYVFLVMEALIDAAVHLGFARHIAHDLVIETLEGSTLF
                     AKQSGLHPAQLRNMVTSPGGTSAAALHELESGRLRTVLSEAVWAAFRRTVELGEQLEA
                     GVPAATDGSDRG"
     gene            complement(4062..4349)
                     /locus_tag="VIF63_10060"
     CDS             complement(4062..4349)
                     /locus_tag="VIF63_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY8239767.1"
                     /translation="MAYVIAEPCIDVLDISCVSVCPVDCIHYEAGTDRKLFIDPNECI
                     DCGACEPECPVNAIFPEDQVPAEWANYTQIDATWFTDRDAARAAVDAAKPR"
     gene            4564..5715
                     /locus_tag="VIF63_10065"
     CDS             4564..5715
                     /locus_tag="VIF63_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8239768.1"
                     /translation="MAILTRADPAKVGTPGAPGAPGAAPAAVRLEHLVKRFSGEVGRR
                     VAPSTGASVAGSGDVAAVDGVDLEIADGEFFSMLGPSGSGKTTTLRLIAGFELPTEGR
                     VWLHGVDVTSRPPFERDVNTVFQDYALFPHMTVGDNVAYGLTVRKVPKPERERRVGEA
                     MAMVRLDGYERRKPGQLSGGQRQRVALARALVNRPRVLLLDEPLGALDLKLREEMQLE
                     LKAIQQEVGITFIYVTHDQEEALTMSDRLAVFNRGRIEQLGTPADLYERPATAFVAGF
                     VGTSNLLRGDAARALVGQEGLFTVRPEKIRMAESGAAVADDEVGADGTIRNVVYLGPD
                     TRYIVALDAGSDLVVTQQNLHTSSMEVLAAQGQPVRLVWKRQHVLRVNE"
     gene            5746..6993
                     /locus_tag="VIF63_10070"
     CDS             5746..6993
                     /locus_tag="VIF63_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8239769.1"
                     /translation="MFQRRLGGALAVAAIVAAACTGGSPTTAPPTTAPVTVAPATSGP
                     TTAPTEAPVATIGPGEGELKIVIWDGYAERGGVDPAYDWVTPFETATGCMVTSQNMID
                     SNNGVGLIESGEFDGISASGDATTRLITGGLVAPVDTRMLPNYANVFPGLKDLPHNTV
                     DGVNYGVPHGRGPNLLGYNTEAVTTAPTSWDGIWEGSAPYKGKISIYDSSIFIADAAL
                     HLMKTKPELGIKDPYQLTQAQFDEAIALLEILDGTTDEEKPNYWGLPADQVAAYTSGD
                     VVIGTTWLYQVNLLTADSQPIAAVLPDEGSTGWSDTWMLAKAAKHPNCMLMWMDHMMS
                     AEANAQATVWFGEAPTSPQACVAAEAISVGHCDQMHATDEAYFSKVHYWATPQTDCKD
                     ADAATTCVSQDDWVNAWSTIRGT"
     gene            7085..>7465
                     /locus_tag="VIF63_10075"
     CDS             7085..>7465
                     /locus_tag="VIF63_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239770.1"
                     /translation="MSTANPPIPTIPTSARRRLAIWLHGRRRVQLGLLLGTPLGWLAI
                     AYLGSLAILLMSAFWTKNPSTSKVEPFTWSLKAFETLFAGDVYQIIALRTIGMAALVT
                     VTCAVLALPIAYYMVRIASPRKRAA"
BASE COUNT         1126 a         2661 c         2592 g         1086 t
ORIGIN      
        1 ccgccgccgt agccggtggc accgatgatc ccgaccttca ccattcgagc catggccgac
       61 gactatagtg cgccggtgag cgccggcccc cagcagcccg gtgacctgcc ggatctccag
      121 ctgcccgagc cgggcacccg cctcggcgcg gtgagcgtcc gggtgtccgc cgcgctcgac
      181 ctcgccgagc tcatcacgac cctccgcgac gccggcgccg agatcgtcgc gatcaacacc
      241 ttcccggcga ccaagggcga gcccgatgtc gcccgcgtct tcgatatcga gacgcgcaac
      301 ttcgacagcg cggcggcggc tcgtgtcctg cggaaggtgc ccggcgtcat caaggccgaa
      361 gcgcgcaaga cgctcggcca gatcttcggc aagcgggtga tcgtgatcgg cggcggcgcc
      421 caggtggccc aggtcaacct cggcgcggtc acggaggcgg atcggcacaa cctccgcggc
      481 gagcgcatca gcgtcgacac catcgcgctc gtcggcgaga agcagatcgc cgatgccgtc
      541 cgcgcggtcg ccgacctgcc ccgggcccac atcctcgtgc tcgccggctc gatcatgggc
      601 ggcgagatca cgaaggccgt gcgtgagctg cgcgaggtcg gcatcccggt gatctgcctg
      661 aacatggccg gatcagtgcc cagcgcggtc gacctggtgg tctcggaccc ggtgcaggcg
      721 ggaacgatgg ccgtgatggc gatcgccgac acggcccggt tctccctcgc ccgccagcgc
      781 ggccggcggt actgatggac gcccgaccga gcgacgctcg acccatggac gctcgcgaga
      841 cgggccggcg ctacctgtcg ccggtcatgg cccgctactt cgagcgcgac tggagccacg
      901 gcgagggcca ccggctgttc gacgtcgacg gccgcagcta cctcgatttc gcgaccggca
      961 tcgcgacgac gatcctcggc catcgacacc ctcgcgtcag tgccgctgtg catgcccagg
     1021 tcgatcggct gagccacgtc atgaacggca tgggctatgc cgagccggta tcgcggctcg
     1081 cggaacgcct cgtcgcgacg ctgcccgcgc cactcgacag cgtgttcttc ggcaactccg
     1141 gcgccgaggc gatcgagggc gcgctcaagc tggcccgccg ggcgaccggc cggccggcga
     1201 tcgtcgcgtt cgagggcggt ttccacggcc ggacctacgg cgcgttgagc gtcaccagct
     1261 cgaaccccaa ctaccggacc ggccatgggc cgctcctccc cgacatccac ctcctgccct
     1321 atccgctcgc gtaccgcgag cacggcggcg atgaagctgc cgcgaccgcc gccgcgctcg
     1381 gcgcccttga cgccttcctg acgacggacg tcgagccccg aacggtcgcg gcgttcctca
     1441 tcgagcccga gctgggcgag ggcggctacg tcccggcgcc gatcgagttc ctccagggcc
     1501 tgcgggcgcg agccgatcag cacgggatcc tgctcatcgc cgacgaggtc cagaccggct
     1561 acgggcgcac cgggcgaatg tggggcttcg agcacgcggg gatcgtcccg gacgtcgtct
     1621 gcgcggccaa gggcatcgcc aacgggctgc cgttgagcgc gatcgtcgcg cgccgcgagc
     1681 tccacgagcg ctggggcaag ggcgcccacg gctctacgtt cgggggcaac ccggtgtcat
     1741 gcgcggctgg aatcgcggtc ctcgagacga tcgaagcgga gggcctcgtt gcaagcgctg
     1801 cggagcgcgg cgagcagctc cgcggcggcc ttcaggccct tgcagccgcc gacgcccgga
     1861 tcggggacgt ccgcggacgg ggcctgatgc tcggggtcga gctggtgcgc gatccggcga
     1921 ccctcgaacc cgacggtgcc ctcgccgacg cgctgattgc gggctgcgcc gacgccgggc
     1981 tcctgctcct cacgtgcggg tcggcccaca acatcgtccg ctggctcgcc ccgctggacg
     2041 tcaacgcggc cgagatcgac gagggcctgg ggatcttcgc ccgggtgctc gcggccgcct
     2101 gaccggccgg gccgcccatg ccacactgcg cccatgggtg cgctcgaaag tcaagcagcc
     2161 aggctcgagg cggcggccgc cgcgatcgtc gcgatgcgcc cgaatgtcga ggccggcgcg
     2221 ccatggccgc tggccgaggt cttcggtccc gagcccgagg ccagctgggg cccacccgag
     2281 ctgctcgccc acgtcgagga gtacctccgc tactggatgg gcgagatcga gcgcgtcctc
     2341 gcggccgatc caggcacgcc cgcgccattc ggacgcgtgg cgaccgacac gatccgcctg
     2401 ggcgtgatcg gccgcgacag gacgctgccg ttccgcgagc tcttcggccg gatcgaggct
     2461 gacgccgcgc gggtcgcgaa gcggcttcgc gagctctccg atgacgatgc gtcgcgccgt
     2521 ggcgtgcacc cggcccgcgg cgagatggcc gtccgcgagc tgctcgagcc gttcctcgtc
     2581 gagcactcgg aagggcacgt caagcagctg cgcgagatcc tcgccacgtc gcgctaggac
     2641 atggcttggt gatgctcgtg gcgatcccgg tcgggatcgt cacgggatgg gtcctccgcg
     2701 ggaacatcga tgggctggcc ggtctccggc tccgctgggc ctggctggcc gtggccggac
     2761 tggccgtgca ggcggtcctc ttcaccgagc gcggaatgga cctcgccgga aacgccgtcc
     2821 ctccgatcta cgtcgtctcg acgctcgcgg tgttcgcgat ggtcgcccgg aatatccgcg
     2881 tcaccggcat gccggtcgtg gccctgggat cgctctcgaa tctcgcggcc atcgtcgcca
     2941 atggtggcgt gatgccggcc gatcccgacg cgatcgtcgc ggccggcctc cagggcgact
     3001 acgccaacag cgtcgcgctg cccgatccag ccctgcggcc gctgaccgac atcttcgcca
     3061 ttcccgcggg cgttccgctc gcgaacgtgt tcagcgtggg ggatgtgctg atcgcgctcg
     3121 ggatcgtgat cgtcattgcc gcggcgatgc ggcgaagccg gtcgacggcg gcgtcaaccg
     3181 ccgcctgagc cgtcagccgc ggtccgagcc gtccgttgcg gccggcacgc cggcctcgag
     3241 ctgctcgccg agctcgacgg ttcggcggaa cgcggcccag acggcctcgg agaggacggt
     3301 tcgaagccgg ccggactcca gctcgtggag cgccgcggcg ctggtgccgc cgggtgaggt
     3361 gaccatgttc cgcagctggg ccgggtgcaa ccccgactgc ttggcaaaga gcgtgctgcc
     3421 ctccagcgtc tcgatgacca ggtcgtgggc gatgtggcgc gcgaagccga ggtggaccgc
     3481 ggcgtcgatg agcgcctcca tcacgaggaa cacgtaggtc gggccggtgc cagagacggc
     3541 agtcgccatc gcgacgagct tctcgtcgtc gacctcgatc tgggcgccga gcgcgctcag
     3601 catgctgccc gcctgctcgc gctgcgctgc cgacgtctcg ggcgtcgcgt accagacggt
     3661 catgccgcgc ccgagctggg ccggggtgtt gggcatgctc cgaacgacct cgcgatgccc
     3721 gaggtggccg atcagggcct gcgtcgtcgc gcccgcgagg atgctgatga ccagctggcc
     3781 gggctggagg cgacctcgaa tctcgcgacc gacgcgggcc agcatctggg gcttgatcgc
     3841 cagcagcacg acgtccgcgc catcgacggc ggccgcgttc gaatcgaccg tgcgcacgcc
     3901 gtgggtccgg gtgagggcgt cgcggcgatc accgcgcgga tgcgatgcca cgatctggtc
     3961 gggcgcgacg agctggccgc ggagcagccc ggcgatgatc gcctcggcca tcacccccga
     4021 gccgacggtt gcaatcgtcc ggcccgcgag ggaggacatg gtcagcgcgg ttttgcggcg
     4081 tccacggcgg cgcgggctgc gtcgcggtcg gtgaaccagg tggcgtcgat ctgggtgtag
     4141 ttcgcccatt cggccgggac ctggtcctcg gggaagatcg cgttcaccgg gcactcgggc
     4201 tcgcaggcgc cgcagtcgat gcactcgttg gggtcgatga acagcttgcg gtccgtgccc
     4261 gcttcgtagt ggatgcagtc cacgggacag accgagacgc aggagatgtc cagcacgtcg
     4321 atgcatggct cggcgatgac gtacgccatg aagcgttgga ctcctctcga ttgagcgtgc
     4381 cgcccgattg gctcggtagg gtagcaccga gtctcccggc cttgacatcg gatcggccgc
     4441 gcccggacac tccgctgcac ctcgcacgat cgcgagctgc gaacctcgcg ctggagcgac
     4501 gcgggaggtt cgaatttcgc ccccgagcgt gccgcgatcg acgcaaggcg cacaaggagt
     4561 cacttggcca tcctgaccag ggcagacccc gcgaaggtcg gcacaccggg cgcaccgggc
     4621 gcaccgggcg cggcgccggc tgccgtgcgc ctcgagcacc tcgtgaagcg cttctccggc
     4681 gaggtcggtc ggcgcgtcgc accttcgacc ggcgcctcgg tcgccggctc tggcgacgtc
     4741 gcggcggtgg acggcgtcga tctcgagatc gccgacggcg agttcttctc gatgctcggc
     4801 ccgtcgggct cgggcaagac gacgacgctg cgcctgatcg ccggcttcga gctcccgacc
     4861 gagggccgcg tctggctgca cggtgtcgac gtgaccagcc gaccaccgtt cgagcgcgac
     4921 gtcaacaccg tcttccagga ctacgcgctt ttcccgcaca tgacggtcgg cgacaacgtt
     4981 gcctacgggc tgaccgtgcg caaggtcccg aagcccgagc gcgaacgacg cgtgggcgag
     5041 gcgatggcga tggtcaggct cgacggctac gagcgccgca agccgggcca gctctcgggc
     5101 gggcagcggc agcgcgtcgc gctcgcgcgg gcactcgtca accgtccccg cgtcctgctg
     5161 ctcgacgagc ccctcggcgc cctcgacctg aagctgcgcg aggagatgca gctcgagctc
     5221 aaggcgatcc agcaggaggt cgggatcacg ttcatctacg tgacccacga ccaggaggag
     5281 gcgctcacca tgagcgaccg cctggccgtg ttcaaccgcg gccggatcga gcagctgggc
     5341 acgcccgccg acctgtacga gcgccccgcg accgcattcg tcgccggctt cgtcggcacg
     5401 tccaacctgc tccgcggcga cgccgcgagg gcgctcgtgg gccaggaagg cctgttcacg
     5461 gtgcgccccg agaagatccg gatggccgaa tccggtgctg cagttgccga cgacgaggtc
     5521 ggcgcggatg gcaccattcg gaacgtcgtg tatctcgggc cggatacgcg ctacatcgtg
     5581 gcgctcgacg ccggctcgga cctggtcgtc acgcagcaga acctgcacac atcgtccatg
     5641 gaggtactgg ccgcacaggg acaacccgtg cggctggtct ggaagcgaca gcacgttctt
     5701 cgcgtcaacg agtgacgcac ggttcgggct gaagaggagg agcgaatgtt ccagcgaaga
     5761 ctcgggggag cgctcgcggt ggccgcgatc gtggccgctg cctgtacagg cggctcgccc
     5821 acgactgccc cgcccacaac ggccccggtc accgtggcgc ctgcgacgtc aggccccacg
     5881 accgccccga ccgaggcgcc ggtggccacc atcggccccg gcgaaggtga gctcaagatc
     5941 gtgatctggg acggctacgc cgagcgcggc ggggtcgatc cggcctacga ctgggtcacc
     6001 cccttcgaga cggccaccgg ctgcatggtc accagccaga acatgatcga ctcgaacaac
     6061 ggcgtcgggc tgatcgagag cggcgagttc gacggcatct cggcgtccgg cgatgcaacg
     6121 acccgcctca tcacgggcgg cctcgtcgcg ccggtcgaca cgcgcatgct cccgaactac
     6181 gccaacgtct tcccgggcct caaggacctg ccgcacaaca cggtggacgg cgtgaactac
     6241 ggcgttccgc atggccgcgg cccgaacctg ctcggctaca acaccgaagc cgtgaccacg
     6301 gcgccgacct cgtgggacgg catctgggag ggcagcgcgc catacaaggg caagatcagc
     6361 atctatgact cgtcgatctt catcgccgat gccgcgttgc acctgatgaa gacgaagccc
     6421 gagctcggca tcaaggatcc ctaccagctg acgcaggcgc agttcgacga ggcgatcgcg
     6481 ctgctcgaga tcctcgacgg cacgacggat gaggagaagc ccaactactg gggtctcccc
     6541 gccgaccagg ttgccgcgta cacctcgggc gacgtcgtca tcggcaccac ctggctgtac
     6601 caggtcaacc tcctgaccgc ggacagccag ccgatcgcgg ccgtcctccc agacgagggc
     6661 tcaacgggct ggtccgacac ctggatgctg gcgaaggctg ccaagcaccc caactgcatg
     6721 ctcatgtgga tggaccacat gatgtcggcg gaggcgaacg cccaggcgac agtctggttc
     6781 ggcgaggcgc cgacgagccc gcaggcctgc gtggccgcgg aagcgatctc tgtgggccac
     6841 tgcgaccaga tgcacgccac ggacgaggcg tacttcagca aggtccatta ctgggccacg
     6901 ccgcagacgg actgcaagga cgctgacgcg gcgaccacgt gcgtgagcca ggacgactgg
     6961 gtcaacgcct ggtcgaccat tcgcggtacc tgatccgggc gaaccacgga acgagcgggg
     7021 ctcctcgagc gatcggggag ccccgtttcg tcctcgactg accaccacgt acgagccgga
     7081 cggcatgagc acagccaacc cgcccatccc gacgatcccg accagcgccc gacggcgcct
     7141 ggcgatctgg ctgcatggcc gtcgccgggt ccagctcggg ctgctcctgg ggacgccgct
     7201 cggctggctg gcgatcgcct acctggggtc gttggcaatc ctgctcatga gcgcgttctg
     7261 gacgaagaac ccgtcaacca gcaaggtcga gccgttcacc tggagcctga aagccttcga
     7321 gacgctgttc gccggcgatg tctaccagat catcgccctg cgcaccatcg gcatggcggc
     7381 gctggtgacc gtcacctgcg cagtcctcgc cctgccgatc gcctactaca tggtccggat
     7441 cgcctcgccc cgcaagcgag cggcg
//
LOCUS       DATBSW010000368         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_316757, whole genome shotgun sequence.
ACCESSION   DATBSW010000368 DATBSW010000000
VERSION     DATBSW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316757"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VIF63_10080"
     CDS             complement(<1..891)
                     /locus_tag="VIF63_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8239771.1"
                     /translation="MTQDTLDARVDARVLADTLWAELPHAVLRQVLHGASVRAVPARG
                     TIFGIGEPGPRAGILLFGTARGFLAAADGRQLTVRYSRRGAFIGRRSYLLGGHAPVAT
                     HAVTDVEILDLEAPRFLQLAKTEVAVSVALLAELSRRLEDVHATVADGAFGSIRERVA
                     RHLLALTDDREPDGRRVARITQQELADGVGTMREVVARALGGFRKEGLIATSAGQIEI
                     LDATSLAASLGAWQVAASGLGPDLRQDVEAILEASPTAIIAVNPDGVIRYVNGLAEAM
                     FGVGRSDVVGRQVEILLPEHL"
     gene            1094..1459
                     /locus_tag="VIF63_10085"
     CDS             1094..1459
                     /locus_tag="VIF63_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239772.1"
                     /translation="MAAVLTFGAVYGMAASLNLATDSLGAANTTVAACQAAQLTATYT
                     STYSAGTPGYTVGTVTVTGLAATCYSKPYKITLSTAGNASLGEATGTTPASGTSFAAT
                     FSPVVSAASVTGISVVISG"
     gene            1545..1916
                     /locus_tag="VIF63_10090"
     CDS             1545..1916
                     /locus_tag="VIF63_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239773.1"
                     /translation="MAGLRAVALAIGTAFAAVTVALAASVNLASSTLAMGSLSVPRCT
                     AAGLTVFPNLTGANVVSVVVGSLPAGCGTSTLQVTVVAGAASGSGSAAVPGGGGSVTV
                     ALGTTVAAAVSERIDLVLVGP"
     gene            1937..2647
                     /locus_tag="VIF63_10095"
     CDS             1937..2647
                     /locus_tag="VIF63_10095"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEY8239774.1"
                     /translation="MFVGTAVLLAVLALLVGLMVGHAAGLNLASQTLTPYRTCVITAT
                     PASTTAVVDASVRQGTANGNFGTTNTVDVASTNAANRRLYLRFDLTVCSPAIPATATV
                     RLATLRLYATALSAACRTIDLFRVTAAWTETTLTWVNQPFGTTLNNPASGTAVDTFTL
                     GTPAGCQNQATGIYMVGANPTADVAAFVAGTATNNGWMLRDDVEGSATARTETFSAKD
                     LGTLAQAPQLVITYVLVP"
     gene            2644..3192
                     /locus_tag="VIF63_10100"
     CDS             2644..3192
                     /locus_tag="VIF63_10100"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8239775.1"
                     /translation="MKLRKLWKVASFGISVAVLAVWWILLRPLALGGPASYVVIRGDS
                     MLPGYTTGDLVVMHAADTYAIGDVVGYHVPAGELGQGLLVVHRIVGGDAVTGFVLQGD
                     NNPAPDPWLPTPDLIDGTPWIAVPGLGRLVSFLHQPAALAALAVSLLVGWQAGRNPAR
                     RPAARVVADAPFVPPQTGPAGT"
     gene            3224..3928
                     /locus_tag="VIF63_10105"
     CDS             3224..3928
                     /locus_tag="VIF63_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8239776.1"
                     /translation="MDRSRNDYYRILQVDPSANPLVIQAAYRVLARIFHPDVQGDDDE
                     QMKRLNRAWEVLGDPRRRAAYDSERAGRHTGPVASTSPAATARHTSADAPSTRTRSDD
                     LHAAFAANAAARDAAAAGAQRPAPPPPPPSPAPASPAPTSRGSEDHAGPPRGEPYGPV
                     LTFGRYEGWSLGQVARVDREFLEWLKRAPAGRSLKHEIDAVLRSMGISGVDRRRYDAD
                     VQRVHTWAPGAPTRIR"
     gene            3992..>4836
                     /locus_tag="VIF63_10110"
     CDS             3992..>4836
                     /locus_tag="VIF63_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8239777.1"
                     /translation="METPPPAHRVAIIGGGFGGLYAARRLGKDERIDLTLVDRRNHHV
                     FQPLLYQVATGALAPGDMAQPLRSILRRNRNTAVLLGEAIGIDVERHEVTLSDGGPIP
                     YDTLVVATGAHHSYFGHDEWASFAQGLKTIDDALEVRRRILIAFEAAEREHVPERRRE
                     WMTFVVVGGGPTGVELAGALGEIARDTLRRDFRSINSADSSILLVEAMDRVLPTYPPD
                     RSASARRQLERLGVRVLTGTKVTDIAEHDVTIDPADPTSASSTIPTRTVLWAAGVQAS
                     SFARKV"
BASE COUNT          699 a         1789 c         1659 g          689 t
ORIGIN      
        1 aaggtgctcc ggaaggagga tctcgacctg cctgccgacc acgtcggacc gcccgacgcc
       61 gaacatggcc tcggccagcc cgttcacgta ccggatcacc ccgtccgggt tgacggcgat
      121 gatcgccgtc gggctcgcct cgaggatcgc ctccacgtcc tggcgcaggt ccgggccgag
      181 gcctgacgcg gccacctgcc aggccccgag ggaagccgcg aggctcgtgg cgtcgaggat
      241 ctcgatctga ccggcggagg tcgcgatcag gccctccttt cggaacccgc cgagcgcccg
      301 ggcaacgacc tcgcgcatcg tcccgacgcc gtccgcaagc tcctgctgcg tgatccgcgc
      361 gacgcgccgc ccgtcaggtt cgcggtcgtc ggtgagggcg agcaggtgcc tcgcgacgcg
      421 ctcgcggatg gatccgaagg cgccatcggc gaccgtggca tggacgtcct cgagccgccg
      481 gctcagctcg gcaaggaggg ccacggagac ggcgacctcg gtcttggcca gctgcaggaa
      541 ccggggggct tcgagatcga ggatctcgac gtccgtcacg gcgtgcgtcg cgacgggagc
      601 gtggcccccg agcaggtacg agcgccgccc gatgaacgcg ccgcggcgtg agtagcggac
      661 ggtgagctgc cggccatccg ccgcggcgag gaagccgcgg gccgtgccga agagcaggat
      721 gccggcccgc gggcccggtt cgccaatgcc gaagatcgtc cctcgcgcgg gcacggcccg
      781 aacgctcgcg ccgtgcagca cctgccgcag gacggcgtgc gggagctccg cccagagcgt
      841 gtccgcgagg actcgcgcgt cgacgcgcgc atcgagcgtg tcctgggtca tgggcctctt
      901 tcgagtcgtc cgatcgaccg ggtcgcagcg cagttgtgac ctatgtcacg gaccgttgct
      961 gaacgtcgcg caccaagcgg gggattacag cgacattaca acgttgcgat ggtgccacag
     1021 aaattttccc caggtagggg caatctgaca ggttgcagtg aggtccggtg tgaagcgcat
     1081 tctggtcatg gtgatggcag ccgttctgac gttcggcgct gtctacggta tggcggcgag
     1141 cctgaacctg gccaccgaca gcctcggggc tgcgaacacc acggtcgcgg cctgccaggc
     1201 agcccagttg acggcgacct acacgtcgac gtactcggca ggcacgcccg gctacaccgt
     1261 cggcacggtg acggtcaccg gtctggccgc cacgtgctac tcgaagccgt acaagatcac
     1321 gctctccacg gccggcaatg catcgctcgg cgaggcaacc ggtacgaccc cggcgtccgg
     1381 cacgagcttc gcggcgacgt tctcgcccgt ggtcagcgcg gcctccgtca ccggcatctc
     1441 ggtcgtcatc agcggctagg ccccgggccg gcgggcggac tcgagcactg gtcccgcgac
     1501 caggcccgaa accgcccgcc ggcccgcgct gcccgcccat ctggatggca ggactccgcg
     1561 ccgtcgcgct cgcgatcgga acggcgttcg ctgccgtaac cgtcgcgctg gcggcgtcgg
     1621 tcaatctcgc gagcagcacg ctcgcgatgg gttccctgag cgtgccccgc tgcactgcgg
     1681 ctggcctcac cgtgttcccg aacctgaccg gcgcaaatgt cgtgagcgtc gtggtcgggt
     1741 cgctgcccgc ggggtgcggc accagcaccc tgcaggtcac ggtcgtcgcc ggcgccgcct
     1801 ctggcagcgg cagtgcggcc gtccccggtg ggggcggctc ggtcacggtc gccctcggaa
     1861 cgaccgttgc ggccgcggtc agcgagcgca tcgatctcgt cctggtcgga ccatgagcgc
     1921 cgcggatcga cggtcggtgt tcgtcggaac ggcggtgctc ctggccgtcc tcgccctgtt
     1981 ggtcggcctg atggtcggtc acgccgccgg cctgaacctc gcatcacaga ccctcacgcc
     2041 gtatcgaacc tgcgtgatca cggcgacacc ggcgtccacg acggccgtgg tcgacgcgtc
     2101 ggttcggcag ggaacggcga atggcaactt cggcacgacg aacacggtcg acgtcgcatc
     2161 caccaacgcc gccaatcgac ggctgtacct gcgcttcgac ctcaccgtgt gcagcccggc
     2221 gatcccggcg accgccaccg tccgcctcgc gacgctccgg ctgtacgcca cggcgctctc
     2281 cgccgcgtgc cgcacgatcg acctgttccg ggtcacggcg gcctggaccg agaccaccct
     2341 cacctgggtc aaccagccgt tcggcacgac gctcaacaat cccgccagcg gcacggcggt
     2401 cgacacgttc acgctcggaa cgccggcggg ctgccagaac caggccaccg gcatctacat
     2461 ggtcggtgcc aatccgacgg ccgacgtcgc ggcgttcgtc gccggcaccg ccacgaacaa
     2521 cggctggatg ctccgcgacg acgtcgaggg ctcggccacg gcgcgtaccg aaaccttctc
     2581 ggcgaaggac ctgggcacgc tcgcgcaggc gccgcagctc gtcatcacct acgtgctcgt
     2641 cccatgaagc ttcggaagct ctggaaggtc gcctcgttcg gcatcagcgt tgccgtgctg
     2701 gcggtgtggt ggatcctgct gcggccgctg gcgctcggtg ggccggcgag ctacgtggtg
     2761 atccggggcg attcgatgct gcccggctat accaccgggg acctcgtcgt catgcacgca
     2821 gcggacacgt atgcgatcgg cgatgtcgtc ggctaccacg tcccggccgg cgagctcggg
     2881 cagggcttgc tcgtggtgca ccggatcgtc ggcggcgacg ccgtgaccgg cttcgtcctc
     2941 cagggcgaca acaacccggc cccggatccg tggctgccca cgcccgacct gatcgacgga
     3001 accccctgga tcgccgtccc gggcctgggc cggttggttt cgttcctcca ccagccggcc
     3061 gcgctggccg cgctggcggt gagcctcctc gtcggttggc aggcggggcg caatccagcc
     3121 aggcgccccg cggctcgggt cgtcgccgac gcgccgttcg taccgcccca aaccggaccg
     3181 gcaggcacct gacttcccgt tgcggccggc gcatcctgcg gccgtggatc gctcgcgaaa
     3241 cgactactac cgaatactgc aggtggaccc ctcggcgaat ccgctggtca tccaggctgc
     3301 gtaccgcgtc ctggcccgga tcttccatcc cgacgtccag ggcgacgacg acgagcagat
     3361 gaagcgcctc aaccgcgcct gggaggtcct cggcgacccg cgccggcgtg ccgcgtacga
     3421 cagcgaacga gcgggccgcc acacggggcc cgtcgcgtcg acgtcgccag ccgcgaccgc
     3481 ccgacacacc tcggcggacg ccccgtcaac gcggactcgc agcgacgacc tccacgcggc
     3541 gttcgccgcg aatgcggccg cacgtgacgc cgccgcggca ggcgcccaga ggcccgcccc
     3601 gccgccgcct ccaccctcgc ctgcgccagc ctcgcctgcg ccaacctcgc gcgggtccga
     3661 ggatcacgcc gggccaccgc gcggtgagcc gtacgggccg gtgctgacgt tcgggcgcta
     3721 cgagggctgg tcgctcggac aggtggcgcg cgtggaccgg gagttcctcg agtggctcaa
     3781 gcgcgccccc gccgggcgca gcctgaagca cgagatcgat gccgtcctcc gcagcatggg
     3841 catctccggt gtcgaccgcc gtcgctacga cgccgatgtg caacgcgtcc acacctgggc
     3901 gccgggcgcc ccgacgagga tccgctagcc gacggatgcc cgccggcccg ccggaccagc
     3961 acgctcgagg aacctcggcc aaacttgacc catggagacg ccgccgccgg cccaccgcgt
     4021 ggcaatcatc ggcggcggat tcggcgggct gtacgcggcg cgtcgcctcg gcaaggacga
     4081 gcgcatcgac ctgaccctgg tcgatcggcg caaccaccac gtcttccagc cgctgctgta
     4141 ccaggtcgcg acgggcgcgc tcgcgcccgg tgacatggcg cagccgctgc ggagcatcct
     4201 gcgtcggaac cgcaacacgg cggtgctgct cggggaggcg atcggcatag acgtcgagcg
     4261 ccacgaggtc accctctccg atggcgggcc gatcccgtac gacaccctgg tcgtcgcgac
     4321 gggtgcgcac cattcgtatt tcgggcacga cgagtgggcg agcttcgcgc agggcctcaa
     4381 gacgatcgac gacgcgctcg aggtccgccg caggatcctg atcgccttcg aggccgcgga
     4441 gcgcgagcac gtgccggagc gccgacggga gtggatgacg ttcgtcgtcg tgggcggcgg
     4501 cccgaccggc gtcgagctcg ccggtgcgct cggcgagatc gcccgggaca cgctccggcg
     4561 cgactttcga tcgatcaact ctgccgactc atcgatcctc ctcgtcgagg cgatggatcg
     4621 cgtcctgccg acctacccgc cggaccggtc cgcctccgcc aggcgccagc tcgagcggct
     4681 cggcgttcgc gtcctgaccg ggacgaaggt caccgacatc gcggagcacg acgtcaccat
     4741 cgacccggcc gacccgacga gcgcctcgag cacgatcccg acgcgaacgg tcctctgggc
     4801 ggcgggcgtc caggcttcct cgttcgcccg caaggt
//
LOCUS       DATBSW010000369        13347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4167 k141_282028, whole genome shotgun sequence.
ACCESSION   DATBSW010000369 DATBSW010000000
VERSION     DATBSW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959065
            Sequence Read Archive: SRR11673319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:16:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,035
            CDSs (total)                      :: 1,998
            Genes (coding)                    :: 1,993
            CDSs (with protein)               :: 1,993
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13347
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282028"
                     /isolate="SMAG_U4167"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VIF63_10115"
     CDS             complement(<1..360)
                     /locus_tag="VIF63_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY8239778.1"
                     /translation="MLQRHHRPEQRSVTRRRRDRGRGQSLVEFSLVFPLIVLLVVAFV
                     EVGRAVYAFNTIANAARQAARVAAVNQLSVVTDCDETRPVEDPYEPHWSIRGCAIAAA
                     TTLNITTANVSVAYTAPP"
     gene            complement(321..1163)
                     /locus_tag="VIF63_10120"
     CDS             complement(321..1163)
                     /locus_tag="VIF63_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY8239779.1"
                     /translation="MQTRSTTPTNRARKSHGQSLVEFALTLPMVLLMVLFGLDFGRVF
                     LGWVALNQAAREAANYAAMNPTAWTLPYNAAVQTEYARLVATEAAQINCVIESPVSAP
                     TFANGTSIGAPATVRLTCQFNLITPFIGILMGNPIPVTASSAFPIRSGNIEGIPVETT
                     TPTATPAPTPTPAPPTPTPAPTAPPAPTPTPAPGATPTPPPAPTPTPTPVPTPTPPTC
                     TVVSLLNLQTNKAAQNWSAAGFTGVVVFSPLVPPQYKIAWQSLTVGTTQLCSSGITVR
                     SSAP"
     gene            complement(1284..2675)
                     /locus_tag="VIF63_10125"
     CDS             complement(1284..2675)
                     /locus_tag="VIF63_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8239780.1"
                     /translation="MSFRARLTAGLVAGSVAPLTVFGLVLLGTEVARTGELDSTLVRV
                     VVFVLAAAVIVTVLFAYLLASNLTAPLRAISRAVERASAGDLSVRVEVAGEDELARLV
                     ESHNRLASDLERRNDELGRILLAIGETSPSDGLDRLVAHAARSARDAFTMLDARIVLG
                     DPTMVPVEEDVPGEAHPVRAVLALPDERLGLLLGRVPATRRWDSADQDLLELYASEVA
                     VAIRNLELFEQVERQRQRLVELDAAKDEFLRGISHNLQTPLTSIRAYAEQMGGERPDP
                     RLSIIVEQADRLYRIVRQLVSVSRLEAGTLRSKGEVLAVAPRVRRAWDALGTAGVPFE
                     LVDDAAGWLAIADPDQLDQVLWALLDNSARYGGGAAIRAHVAPRPTESLLAVTIADGG
                     PGVKPEERDRVFERYERGAAGSGVEGTGLGLYVSRQLCRAMGGDLVLEPAVAGSGAAF
                     TVTVPAEPPATES"
     gene            complement(2747..3499)
                     /locus_tag="VIF63_10130"
     CDS             complement(2747..3499)
                     /locus_tag="VIF63_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8239781.1"
                     /translation="MLTGGWRGSGGGAPRILIVDDDPNLLVLLADQLRADGFEIQTAR
                     DGEEALRRLDSGWPDLLIVDMMMARMDGLTLAREVKSRADLPIIVLSAIDTADSKADL
                     LEEVAEDYVTKPYHYPELRARIQRVMRRLGDKVPRRELALGPDLSLDLRSRTATVKGE
                     VVQLTPTESRLLYALAANLGNTVSTETLLDRGWGDTDDADPSYVWVTMRRLRQKIEPD
                     ADHPIHLVTVRGIGYRLVARDAPPPPAAAAAE"
     gene            complement(3531..4409)
                     /locus_tag="VIF63_10135"
     CDS             complement(3531..4409)
                     /locus_tag="VIF63_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239782.1"
                     /translation="MTLAKRPARAATTKATTKATTRNKKAPKRRARRKPNPYLAWRKR
                     LDRTRPGLIAFARDGLAELYGHHPWVRRWDPTSELVMTILTQNSADINAEHAYQQLRK
                     AYPSGRPIERHVPGPGWGGNGMPDGIPPDWSQVDNAPLEELIEVIRPGGLPNQKAKGI
                     KAALRAIHEARGDYSLEFLGEMSALEARAWLTGIKGVGVKTASVVLLFSFGLPLMPVD
                     RHVERVAYRVGLLPPKATAEEAHDYFLAMLEPDEMHEAHINFIRHGRLVCHAQRPAHD
                     RCPLRDRCRFVDPKAP"
     gene            complement(4406..5551)
                     /locus_tag="VIF63_10140"
     CDS             complement(4406..5551)
                     /locus_tag="VIF63_10140"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY8239783.1"
                     /translation="MRVVIAPDSYKGSLTSVEVATALADGWREARPDDEVLLAPLADG
                     GEGTLVAIAAAGGWEWRDVTATDPIGRPIRARWLRAADGSRAVVELAEASGLSRLAAS
                     ERDPVGASTRGTGEVLRAVLDAGIRVITLGIGGSATTDGGAGILRALGASIEDRAAAK
                     GDRGTAEPVVDLTSLDRRLAEVDLRIACDVTNPLLGARGAAATYGPQKGASGEQVIAL
                     DDALGRYADALAAASGRDERDTPGAGAAGGTAFGLLSIADRFRSMKLVPGVGVVMEET
                     GLREKLAGADLVITGEGRIDAQTAFGKTALGVAQLASAAGVGCICVGGGVEPEGEAVL
                     ATLGAITVSVTEAPMSIEAAIAAGTAPVKRCGRRIARLVSAGRTLTA"
     gene            complement(5552..7495)
                     /locus_tag="VIF63_10145"
     CDS             complement(5552..7495)
                     /locus_tag="VIF63_10145"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEY8239784.1"
                     /translation="MTGPLSGPPSGKPLPMRIVIPVANPATAEELIRLGADLLDPRGG
                     ELTALGIVEVPEGMPLSEGATRARHARRLLQRVLDFVPEGTPIHPIVRIGRHAAEGIV
                     EAAAELEADLIVFGWGGKSPGGRDGLTSPFSMTIDEVLRDAPCDVAVVKQRGVRNLKR
                     VVVPVRGGPHAELALHLGKALAARHDATLAALHVVPPGITLAVRAQAERALTAFVRQH
                     VDGKVETLVREASNIRAAILREADHSDIVVMGAAAPSSVDGTAHLFGAMPEAIATKSQ
                     TTVVVVKTRESIGRQTFEQLATHAETLAAADRAAEESRAVPARVERWFGESNFHHHEF
                     ADLRRLVQLKEKQGLTVSLVLPTLNEEETIGPIVRRAVREMVGRVPLLDEILVVDSAS
                     TDRTREIAESEGARVIQHPDVLSRYGSFIGKGEALWKSLYETSGDLVVWADTDVRNWH
                     PRMVYGTLGPLLHEPRLQYVKGYYQRPIVEAGVLREGGGGRVTELVARPLINLFFPEL
                     SGFIQPLSGEYAGRRSLLEQIPFFTGYAVEIGHLIDVAERAGLDGLGQVDLERRVHRN
                     QELEGLSQMSFVIMQAVMKRIEERRKARLFAEMGSTMKLPRSGRGRLSLEVIELADME
                     RPPMIRIPEYMERRSLLTDSLVG"
     gene            complement(7492..8856)
                     /locus_tag="VIF63_10150"
     CDS             complement(7492..8856)
                     /locus_tag="VIF63_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8239785.1"
                     /translation="MPQPVLTPAIREEIARLERADIMVGIPSFKNAATIGYVVRAAQA
                     GLVQYFPDLRPVVVNSDAGSPDGTGRVVVETEPPDYIERILLVRPTNKLSRVSLTYPE
                     IDGSGGKGAALRTIFEMAAALDVQALVVVDSDLRSILPEWIELLAGPIIKGGYDYVAP
                     LYARHKHDGTITNNVTYPLTRALYGLRIRQPIGGDFGVSGDLVRHYLEAGHWTPEVSK
                     FGIDIWMTTLALNGGFAICQARLGAKIHDPKDPGADLGPMFRQVVTTILRMAVANADR
                     WGPIRGSHDVPAYGFERVLDPPPLEVNVLRLLSEFHAGSLTLARTWSEMLSADTVAAV
                     MGLAEEAGRVADHVREALDMETPGRGPASTAAMADAAAAFAFPDAVWVRVIYDLVITA
                     RDQPEQLEAYVTSLVPVYFGRVASSFIENRDLSTDRAEESIERQAREFERLKPYLVNR
                     WGAA"
     gene            8974..10311
                     /locus_tag="VIF63_10155"
     CDS             8974..10311
                     /locus_tag="VIF63_10155"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8239786.1"
                     /translation="MGLLARAGGLTTSGKARVTVAWSLYDFANTIFSFAVVSSAIGSW
                     LVADERFGERDGNFVLSVAVVLSVGLNALVSPILGALSDRGGRRMPYLLFFTALCIAP
                     TLFIGWSPPVIGVVLFVIANFAYQAALIYYDSTLKSVAKPATRGRLSGIGVAVGYGGT
                     IFAGLFLLILDVAVENRFFVAGVLFAVFAIPIFLFVRESGPIGRVTKADVTGSLGQLR
                     SSIAHAREVPGLLRFLVARFFYSDAVNTIIVVMTIVTTKAKGFTDSQALIVLLSLTVA
                     AIAASFGWGYLVDRLGPRRTLMIVLSSWAVGLILGAISLSVDGTIGVVMFLTAGAILG
                     SGLGGVQVADRVLMVRLSPPARVGEFFGLYGLVGKGSQVIGQMLYGLTLLLFFDILGV
                     GAYQLAVLTLLGTMLIGAWLLRPVTDTWEGAGEIDVHAPPERLAPMSAPLDPR"
     gene            complement(10308..11297)
                     /gene="galE"
                     /locus_tag="VIF63_10160"
     CDS             complement(10308..11297)
                     /gene="galE"
                     /locus_tag="VIF63_10160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY8239787.1"
                     /translation="MRILVTGGAGYVGSVSVEALVAAGHEVTVLDNLATSSGSADLDG
                     ATLVQGSYGDREALAALLTSRAIDAVLHCGARSLVGESVRDPGLYFRENLGGGIAMLS
                     GMQDAGVRRLVISSTAAVYGIPDAAPIPEGAQLRPINPYGETKRSLEAAAAWFASSYG
                     FRVASLRYFNVAGASKRNGEHHVPETHLIPNALTAIETGVPMPLFGTDYPTRDGTAIR
                     DYVHVEDLAEAHRLALEATAPDDERTDAPLIVNLGSGSGYSVRDMLSAARGALDREVP
                     YVEQGRRAGDPPILVAAIDKARDVLGWEPRRSTLEEMISSAWAERRSRATERS"
     gene            complement(11318..11665)
                     /locus_tag="VIF63_10165"
     CDS             complement(11318..11665)
                     /locus_tag="VIF63_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8239788.1"
                     /translation="MIERLLAGEAALARGELDAADRLFTQVTEADPMNAIAVVGRARV
                     ALAHGAREDARAFAERALGLDPEEAAARRLLLELDTPAVMVPAPGPVAAALPETTRRS
                     GWRGWLDRLLRRS"
     gene            complement(11662..13032)
                     /locus_tag="VIF63_10170"
     CDS             complement(11662..13032)
                     /locus_tag="VIF63_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8239789.1"
                     /translation="MTSIDVPESVRAVMERLWSHGHAAYVVGGSVRDAVLGRAAEDWD
                     LATDARPDRLLAIFPGAVYENRFGTVAVREGESQHEVTTFRTDHEYADFRRPHHVEFG
                     DDVRLDLARRDFTVNAIAWGAEAPARAGGVTAAAPEPSIVDPFEGVTDVQREVLRAVG
                     EAAARFQEDALRMVRAVRLAAVLEFTIDPPTLEAIGAHAALAAHVSGERVAAELGKLL
                     SAARPSVGLRLMAETGLLGVLFPDLAAQQGIEQNKIPGEDLFDHSMRSVDAAPAARPV
                     VRLAALLHDIGKPSTLDDGPFRGHELVGADIAQRVLDGLHLPKAATERVVHLVRNHMF
                     TYEPEWGEAGVRRFIQRVGIESIDDLFALREADNIGSGVPADAHGLGELRARVDAELA
                     AAVVLDRSRLAVHGDDLMAELDVPAGPRLGRILDRLLDRVIADPTLNDRPTLLLLAES
                     MLTEDG"
     gene            complement(13078..>13347)
                     /locus_tag="VIF63_10175"
     CDS             complement(13078..>13347)
                     /locus_tag="VIF63_10175"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8239790.1"
                     /translation="CQYCGKVASDLTLDHILPRHRGGSHTWDNLVAACKPCNHRKGGK
                     TPEEARVRLLRVPFEPRSDLYSLFTPYLADERNENWRNYLFLGRN"
BASE COUNT         1878 a         4622 c         4825 g         2022 t
ORIGIN      
        1 gggcggtgcg gtataggcca ccgagacgtt cgcggtggtg atgttcaacg tcgtcgctgc
       61 ggcgatcgcg cagccgcgaa tcgaccagtg cggctcgtac gggtcttcga ccggccgcgt
      121 ttcgtcgcag tcggtgacga cactcagctg gttgacggcc gccactcgcg ctgcctggcg
      181 cgcggcattg gcgatggtgt tgaaggcgta gaccgcccgt ccgacctcga cgaaggcgac
      241 gacgagcaag acaatgaggg ggaatacgag cgagaactcg acgaggctct ggccccgacc
      301 acggtcgcgt cgacgccgcg tcacggagcg ctgctccgga cggtgatgcc gctggagcac
      361 agttgcgtgg tcccgaccgt aaggctctgc caggcgatct tgtactgggg cgggacgagc
      421 ggcgagaaga ccaccacgcc cgtgaagccc gcggcagacc agttctgcgc cgccttattc
      481 gtctgcaggt tcagcaggct cacgacggtg caggtggggg gcgtcggggt tggtaccggc
      541 gtcggtgtcg gtgtcggcgc cggtggaggg gtcggtgtgg cacccggggc cggggttggg
      601 gtcggcgccg gcggcgctgt tggcgcgggt gtcggcgtcg gtggagcagg cgttggcgtg
      661 ggcgcaggcg tggccgtggg cgtcgtcgtc tcgaccggga tgccctcgat gttgccgctc
      721 cggatcggga aggccgagga ggcggtcacc gggatggggt tgcccatcag gatgccgatg
      781 aacggcgtga tcaggttgaa ctggcaggtc agccggacgg tcgcgggcgc cccgatgctc
      841 gtgccgttgg caaaggtcgg cgccgagacc ggtgactcga tgacgcagtt gatctgcgcg
      901 gcctcggttg cgacgaggcg tgcgtactcg gtctggacgg cggcgttgta cgggagcgtc
      961 cacgcggtcg ggttcatcgc ggcgtagttc gcggcttcgc gcgctgcctg gttgagcgcc
     1021 acccagccga ggaacacgcg accgaagtcc aggccgaaca gcaccatgag cagcaccatc
     1081 ggcagcgtca gggcgaactc gacaaggctc tggccgtggc tcttccgggc acggttggtc
     1141 ggcgtcgttg agcgtgtttg catcgagact tcaagtcttc tcaggcaacc tttcgcgtca
     1201 ccttgccagt ggttctgaac ggcaccggcc cttcgtacta gaccgccggg acctatggcg
     1261 gccgggagca cgaatcgagc gcgctacgac tccgttgccg gtggttcggc ggggacggtc
     1321 acggtgaagg cagcgccgga tccggccacc gccggctcga ggacgaggtc gccgcccatc
     1381 gcccggcaca gctgtcgaga cacgtacagc ccgaggccgg tgccctcgac gcccgagccc
     1441 gcggcgccgc gctcgtagcg ttcgaagacg cggtcgcgct cctccggctt caccccgggt
     1501 ccgccgtcgg cgatggtcac cgcgagcagg gactcggtgg ggcgcggcgc cacgtgggcg
     1561 cgaatcgccg ctcccccgcc ataccgcgca gagttgtcga gcaacgccca cagcacctgg
     1621 tcgagctggt cggggtcggc gatcgccagc cagcccgccg cgtcgtcgac gagctcgaac
     1681 ggcacgcctg ccgtgccgag cgcatcccag gcgcggcgca cgcgcggcgc cacggcgagg
     1741 acctcgcctt tcgaccggag cgtgccggct tcgaggcgtg ataccgacac gagctggcga
     1801 acgatccggt acaggcgatc cgcctgctcg acgatgatcg agaggcgcgg gtccgggcgc
     1861 tccccgccca tctgctccgc gtaggcgcgg atgctcgtca gcggcgtctg gaggttgtgg
     1921 ctgatgccgc gcaggaactc gtccttcgcc gcgtccagct cgacgaggcg ctggcgctgg
     1981 cgctccacct gctcgaacag ctcgaggttg cggattgcga cggcaacctc gctcgcgtac
     2041 agctcgagca ggtcctgatc ggccgaatcc cagcgacggg tcgcgggcac gcgaccgagg
     2101 agcagcccga gccgctcgtc gggcagggcg agcacggcgc gcaccggatg ggcctccccg
     2161 ggcacgtcct cctcgaccgg caccatcgtc gggtcgccga ggacgatccg ggcatcgagc
     2221 atcgtgaatg cgtcccgggc gctccgcgcg gcgtgggcga cgagccgatc gagcccgtcg
     2281 ctgggcgaag tctcgccgat cgcgagcagg atccggccga gctcgtcgtt gcggcgctcg
     2341 aggtcgctcg ccaggcggtt atggctctcg acgaggcgcg ccagttcgtc ctcgcccgca
     2401 acctcgacgc gcaccgacag gtcgcccgcg gacgcgcgtt ccacggctcg cgagatcgcc
     2461 cgcagcggtg ccgtgagatt gctcgcgagg aggtaggcga acaggaccgt gacgatcacc
     2521 gccgcggcaa ggacgaagac cacgacacgg accagggtcg agtcgagctc gccggtgcgc
     2581 gccacctcgg tgcccaggag gaccaggccg aacaccgtga gcggcgccac ggaacccgcc
     2641 acgagcccgg cggtgagccg ggcccggaac gacatcgccg ggccgatccg ctgtggctcc
     2701 ggcctcggac ccgaccggtc gatcggcggc cggcggcccg cgttgctcac tcggcggcgg
     2761 cggcggcggg cggcggcggg gcgtcgcgtg ccacgaggcg gtagccgatc ccgcggaccg
     2821 tgacgaggtg gatcggatgg tccgcgtcgg gctcgatctt ctggcgcagc cggcgcatcg
     2881 tcacccacac gtaggagggg tcggcgtcat ccgtgtcgcc ccagccgcga tcgaggagcg
     2941 tctcggtgct gaccgtgttg ccgaggttcg cggcgagggc gtagaggagt cgcgactcgg
     3001 tgggcgtcag ctggacgact tcgcccttga ccgtcgcggt acggctccgg agatcgagcg
     3061 acaggtccgg gcccaacgcg agctcccgcc gtgggacctt gtccccgagt cgacgcatca
     3121 cccgctggat ccgggctcgg agctcggggt agtggtacgg cttggtgacg tagtcctcgg
     3181 cgacctcctc gaggaggtcg gctttcgagt cggcggtgtc gatcgcggac aggacgatga
     3241 tcggcaggtc ggcgcgcgac ttgacctcgc gagcgagggt cagcccgtcc atccgggcca
     3301 tcatcatgtc gacgatcagc aggtccggcc agccgctgtc gagacggcga agcgcttcct
     3361 cgccgtcacg cgcggtctgg atctcgaagc cgtcggcgcg cagctggtcg gcgagcagga
     3421 cgagcaggtt cgggtcgtcg tcgacgatca ggatgcgcgg cgccccgccg ccgctgcctc
     3481 gccaaccgcc cgtgagcatg gcgcgatgat aggtctgcga acccgcccgc ctacggcgcc
     3541 ttcgggtcga cgaagcggca gcggtcgcgc agcgggcatc gatcgtgggc cggtcgttgc
     3601 gcgtggcaga cgaggcggcc gtggcgaatg aagttgatgt gcgcctcgtg catctcgtcg
     3661 ggctcgagca tggcgaggaa gtagtcgtgg gcctcctcgg cggtcgcctt cgggggcagc
     3721 agcccgacgc gataggcgac gcgctcgaca tggcggtcga ccggcatcag tggcaagccg
     3781 aacgagaaga gcagcacgac cgatgccgtc ttcacgccga cgcccttgat cccggtgagc
     3841 cacgcccggg cttcgagggc ggacatctcg ccgaggaact cgagcgagta gtcgccccgc
     3901 gcctcgtgga tcgcccgcag ggccgccttg atgcccttgg ccttctggtt gggcaggccg
     3961 ccgggtcgga tgacctcgat cagctcttcg agtggcgcgt tgtcgacctg cgaccagtcg
     4021 ggcgggatgc catcgggcat cccgttgccg ccccagcctg gaccggggac gtgccgctcg
     4081 atgggtcgac cggagggata ggccttccgc agctgctggt aggcgtgctc ggcattgatg
     4141 tcggcggagt tctgggtgag gatcgtcatg acgagctcgc tcgtcggatc ccagcggcgg
     4201 acccagggat ggtggccgta cagctcggcg aggccgtcgc gggcgaaggc gatcagcccg
     4261 ggccgggtcc ggtcgaggcg cttgcgccag gcgaggtacg ggttcggctt ccggcgggca
     4321 cgccgtttcg gcgccttctt gttgcgggtc gtcgccttgg tcgtcgcctt ggtcgtcgcc
     4381 gccctggccg gccgtttcgc gagggtcacg ctgtcagggt acggcccgcc gagaccagtc
     4441 gagcgatgcg ccggccgcag cgcttgacgg gcgccgtccc ggcggctatt gccgcctcga
     4501 tcgacatcgg cgcttcggtg acgctcaccg tgatcgcgcc gagcgtcgcg agcacggcct
     4561 cgccttcggg ctcgaccccg ccgcccacac agatgcagcc aacgcctgcc gcgctcgcga
     4621 gctgggcgac gccgagcgcg gtcttgccga acgcggtctg ggcgtcgatg cggccctcgc
     4681 ccgtgatcac gaggtcggcg ccggcgagct tctcgcgcag accggtctct tccatcacga
     4741 ccccgacccc cggcacgagc ttcatcgatc ggaagcggtc ggcgatcgag agcaggccga
     4801 acgcggttcc gcccgcggcg ccagccccgg gcgtgtcgcg ctcgtcccga ccgctcgcgg
     4861 cggcaagcgc gtccgcgtag cggccaagcg cgtcgtcgag cgcgatcacc tgctcgcccg
     4921 aggcaccctt ctggggtccg tacgtggcgg ctgcgccccg cgcgccgagc agcgggttgg
     4981 tcacgtcgca ggcaatccgg aggtcgacct cggcgaggcg acggtccagg ctggtcaagt
     5041 cgacgacggg ttccgctgtg ccgcggtcgc ccttcgcggc ggcacgatcc tcgatcgatg
     5101 cgcccagcgc gcgcaggatc ccggcgccgc cgtccgtcgt ggcgctgccg ccgatgccga
     5161 gcgtgatgac acggatcccg gcgtccagca cggcgcgcag gacttcgccc gtcccccgcg
     5221 tcgaggcccc gaccggatca cgctccgatg ccgcgaggcg tgacagcccc gaggcctcag
     5281 ccagctcgac gaccgcgcgc gacccgtcag cggcacgcag ccaacgcgcg cggatcggcc
     5341 ggccaatcgg atccgttgcc gtgacgtcgc gccactccca gccgccggct gcggcgattg
     5401 ccacgagcgt cccctcgccg ccgtcggcga gcggggcgag caggacctcg tcgtcgggcc
     5461 gcgcctcgcg ccagccgtcg gcgagggccg tcgcaacctc gacggacgtg agtgagcctt
     5521 tgtaggagtc cggagcgatg acgacgcgca cttagccgac gagtgagtcg gtgagcagac
     5581 tccggcgctc catgtattcc gggatacgga tcatcggcgg gcgctccatg tcggcgagct
     5641 cgatgacctc gagcgagagc cggccgcggc cggagcgggg gagcttcatg gtcgagccca
     5701 tctcggcaaa caggcgcgcc ttgcggcgct cctcgattcg cttcatcacc gcctgcatga
     5761 tcacgaagct catctgggaa agcccctcga gctcctggtt gcgatggacc cgccgttcga
     5821 ggtcgacctg gccgagcccg tcgagcccgg cccgttcggc cacgtcgatg aggtgcccga
     5881 tctcgaccgc gtagcccgtg aagaacggga tctgctcgag caggctgcgg cggccggcgt
     5941 actcgcccga cagcggctgg atgaagccgg acagctcggg gaagaacagg ttgatcaggg
     6001 gccgcgccac gagctcggtg acgcgcccgc cgccgccctc acggagcaca ccggcctcga
     6061 cgatcgggcg ctggtagtag cccttgacgt actgcaggcg cggctcgtgg agcagcggcc
     6121 ccagggtccc gtagaccatc cgcgggtgcc agttgcgcac gtccgtgtcg gcccagacga
     6181 cgaggtcgcc cgacgtctcg tagagcgact tccacagcgc ctcgcccttg ccgatgaagc
     6241 tgccgtaccg gctcagcacg tcggggtgct ggatgacccg tgcgccttcg gactcggcga
     6301 tctcgcgggt ccgatcggtc gacgccgaat cgacgacgag gatctcgtcg agcagcggca
     6361 cgcgcccgac catctcgcgc acggcgcgcc gaacgatcgg cccgatcgtc tcctcctcgt
     6421 tgagggtcgg caggacgagg ctgaccgtca ggccctgctt ctccttgagc tggacgagcc
     6481 ggcgcaggtc cgcgaactcg tgatggtgga agttcgattc gccgaaccag cgctcgacgc
     6541 gggccggcac ggcgcgcgat tcctcggcag ctcggtcggc cgcggcgagc gtctcggcat
     6601 gcgtggccag ctgctcgaac gtctggcgcc cgatcgactc gcgcgtcttc accacgacca
     6661 cggtcgtctg tgacttcgtc gcgatcgcct cgggcatcgc gccgaacagg tgggcggtgc
     6721 cgtcgacgga gctcggcgcg gcggcgccca tcacgacgat gtccgagtgg tccgcctcgc
     6781 gcaggatcgc ggcgcggatg ttcgaggcct cgcgcacgag cgtctcgacc ttgccatcga
     6841 cgtgctgccg gacgaacgcg gtgagtgcgc gctcggcctg tgcccggacg gccagggtga
     6901 tccccggtgg cacgacgtga agtgccgcga gcgtcgcgtc gtgccgcgcc gcaagcgcct
     6961 tgcccaggtg gagggccagc tcggcgtgcg ggccaccgcg gaccggcacg acgacgcgct
     7021 tcaggttgcg gacgccgcgc tgcttcacca ccgcgacgtc gcacggcgca tcgcggagga
     7081 cttcgtcgat ggtcatcgag aacgggctcg tcaggccgtc gcggccaccg ggcgacttgc
     7141 caccccagcc gaagacgatg aggtccgcct cgagctcggc ggcagcctcg acgatgccct
     7201 cggccgcgtg gcgcccgatg cgcacgatcg ggtggatggg cgtgccctcg ggcacgaagt
     7261 cgagcacgcg ctgcagcagg cgccgggcgt ggcgcgcccg cgtcgcgccc tccgacaggg
     7321 gcatgccctc gggcacctcg acgatgccga gcgcggtcag ctcgccgccg cgtgggtcga
     7381 gcaggtcggc accgagccgg atcagctcct cggcggtcgc cgggttcgcg accggaatga
     7441 cgatccgcat cggcagcggc ttgcccgacg gcgggcccga caacggcccc gtcacgcggc
     7501 accccagcgg ttcacgaggt atggcttgag ccgctcgaac tcgcgcgcct ggcgctcgat
     7561 gctctcctcg gcgcgatcgg tcgacaggtc gcggttctcg atgaaggagc tggcgacccg
     7621 cccgaagtac accggcacga gcgacgtgac gtacgcctcc agctgctccg gctgatcacg
     7681 ggcggtgatc acgaggtcgt agatgacgcg tacccagacc gcatcgggga acgcgaacgc
     7741 ggccgcggcg tcggccatcg cggcggtgct ggcaggcccg cggccgggcg tctccatgtc
     7801 cagcgcctcc cgcacgtggt cggccacgcg acccgcctcc tcggcgagac ccatcacggc
     7861 cgccacggtg tccgcggaga gcatctcgct ccacgtccgg gcgagcgtga gcgacccggc
     7921 atggaactcg gagagcaggc gaagcacgtt gacctcgagc ggcggcggat cgaggactcg
     7981 ctcgaagccg tacgccggga cgtcgtggct gccgcggatg ggcccccagc ggtcggcgtt
     8041 ggcgacggcc atgcgcagga tcgtggtcac gacctgccgg aacatcggcc cgaggtcggc
     8101 gccgggatcc ttgggatcgt ggatcttcgc gcccaggcgg gcctggcaga tggcgaagcc
     8161 gccgttgagc gcgagcgtgg tcatccagat gtcgatgccg aacttggaga cctcgggcgt
     8221 ccaatggccc gcctcgaggt agtggcggac gaggtcgccc gatacgccga agtcgccgcc
     8281 gatcggctgg cggatgcgca gcccgtacag ggcccttgtg agcgggtacg tgacgttgtt
     8341 cgtgatcgtg ccgtcgtgct tgtggcgggc gtagagcggc gcaacgtagt cgtagccgcc
     8401 cttgatgatc ggcccggcca ggagctcgat ccactcgggc aggatcgagc gcaggtcgga
     8461 gtcgacgacg accagcgcct ggacgtcgag cgccgccgcc atctcgaaga tcgtgcgcag
     8521 cgccgcaccc ttgccgcccg agccgtcgat ctcggggtac gtgagcgaga cccgcgacag
     8581 cttgttcgtg ggccgaacga ggaggatccg ctcgatgtag tccggcggct cggtctcgac
     8641 gacgacgcgg cccgtcccgt cgggcgagcc cgcatccgag ttgacgacga ccggccggag
     8701 atcggggaag tactggacca gcccggcctg agccgcccgc acgacatagc cgatcgtcgc
     8761 ggcattcttg aagctcggga ttccgaccat gatgtccgcc cgttcaagtc gggcgatctc
     8821 ttcccggatg gccggcgtca gcacaggctg aggcatcgag cccaccgtac caccgatgca
     8881 catcagcctc gttgcgcgcg catcgcaacg gagcgcgatg tcgtgcccgc gcgcgttgcg
     8941 cgggagccta tgatcgccgg cgggagggcc gccatgggtc tgctcgcgcg cgctggtggg
     9001 ctgacgacct cgggcaaggc ccgggtcacg gtcgcctggt cgctgtacga cttcgcgaac
     9061 acgatcttct cgttcgcggt cgtctcgagc gcgatcggct cgtggctcgt cgccgacgaa
     9121 cgcttcggcg agcgcgacgg caacttcgtc ctgagcgtgg ccgtggtctt gagcgtcggg
     9181 ctcaacgcgc tcgtctcgcc gatcctcggc gcactcagcg atcgcggcgg ccggcgcatg
     9241 ccgtacctgc tcttcttcac ggccctgtgc atcgcgccga cgctgttcat cggctggagc
     9301 ccgccggtca tcggcgtcgt gttgttcgtg atcgcgaact tcgcgtacca ggccgcgctg
     9361 atctactacg actccacgct caagtccgtt gccaagcccg cgacacgcgg ccggctctcg
     9421 ggcatcgggg tcgcggtggg ctatggcggc acgatcttcg ccggcctgtt cctgctgatc
     9481 ctcgacgtgg ccgtcgagaa ccgcttcttc gtcgccggcg tgctgttcgc ggtgttcgcc
     9541 atcccgatct tcctgttcgt gcgcgagtcc ggcccgatcg ggcgggtcac caaggccgac
     9601 gtgacgggct cgctcggcca gctccgctcg agcatcgccc acgcccgcga ggtgcccggc
     9661 ctgctccgct tcctcgtcgc ccgcttcttc tactcggatg ccgtgaacac gatcatcgtc
     9721 gtgatgacca tcgtgaccac gaaggcgaag gggttcaccg actcacaggc cctgatcgtg
     9781 ctgctcagcc tgactgtcgc ggcgatcgcg gcgagcttcg gctggggcta cctcgtcgat
     9841 cggctggggc cgcggcgcac cctgatgatc gtgctgagct cgtgggcagt cggcctgatc
     9901 ctgggtgcca tctcgctcag cgtggacggc acgatcgggg tcgtgatgtt cctcaccgcc
     9961 ggcgcgatcc tcggcagcgg cctcggcggc gtccaggttg ccgatcgagt gctgatggtc
    10021 cggctctcgc cgccggcccg cgtcggcgag ttcttcggcc tgtacggcct cgtcggcaag
    10081 ggctcgcagg tcatcggcca gatgctgtac ggactcacgc tgctgctgtt cttcgacatc
    10141 ctcggcgtcg gtgcgtacca gcttgccgtc ctgacgctgc tcgggacgat gctgatcggg
    10201 gcatggctcc tgcggcccgt cacggacacg tgggagggcg cgggcgagat cgacgtccac
    10261 gcaccgcccg agcgcctcgc accgatgtcg gcgccgctcg acccgcgcta ggaccgctct
    10321 gtggctctcg atcgccgctc ggcccatgcc gagctgatca tctcttcgag cgtgctgcgc
    10381 cggggctccc agcccaggac gtcccttgcc ttgtcgatgg cggcgacgag gatcggcgga
    10441 tcgccggccc ggcgcccctg ttcgacgtag ggcacttcgc gatccagggc gccgcgagct
    10501 gccgacagca tgtcccggac gctgtagccg ctcccgctcc cgagattgac gatgagcggt
    10561 gcgtcggtcc gttcgtcgtc gggcgcggtc gcctcgagcg cgagccgatg ggcctccgca
    10621 aggtcctcga cgtggacgta gtcgcggatg gcggtgccgt cgcgcgtcgg gtagtcggtg
    10681 ccgaagagcg gcatcggcac gccggtttcg atggccgtga gcgcattcgg aatgaggtgt
    10741 gtctcaggca cgtggtgttc gccgttgcgc ttgctcgcgc cggcgacgtt gaaataccgc
    10801 aacgacgcga cgcggaagcc gtacgagctg gcgaaccacg ctgcggcggc ctcgagcgag
    10861 cgcttggtct cgccgtatgg gttgatcggg cggagctgcg cgccctccgg gatcggcgcc
    10921 gcatccggga tgccgtacac ggccgcggtc gatgagatca cgaggcgccg cacgcctgcg
    10981 tcctgcattc ccgagagcat ggcgatcccg ccgccgaggt tctcccggaa gtagagaccc
    11041 gggtcccgga ccgactcgcc gaccagcgag cgcgcgccgc aatggagcac ggcatcaatc
    11101 gcacggctgg tcagcagcgc tgcgagggcc tcgcggtccc cgtagctgcc ctggacgagg
    11161 gtcgcgccgt cgagatcggc cgatccgctg ctcgtcgcga ggttgtcgag gacggtgacc
    11221 tcgtggcccg cagcgacgag cgcctcgacg gacaccgagc cgacgtagcc ggcgccgccg
    11281 gtgacgagga tgcgcattgg cgcagggtag ccgcgcgtca gcttcgtcga aggaggcggt
    11341 ccaaccagcc gcgccagccc gatcgccgcg tcgtctcggg cagcgcggcc gcgaccggac
    11401 ccggcgcggg gaccatgacc gcgggtgtgt ccagctcgag cagcaatcgg cgcgccgccg
    11461 cctcctcggg atcgagcccg agggcgcgct cggcaaaggc ccgcgcgtcc tcgcgggccc
    11521 catgagcaag cgccacgcga gcgcgaccga ccacagcgat ggcattcatc ggatctgctt
    11581 cggtgacctg ggtgaacagc cgatcggcgg cgtcgagctc accgcgcgca agcgccgcct
    11641 cccctgcgag cagccgctcg atcatccgtc ctccgtcagc atcgactccg ccaggagcag
    11701 gagcgtcggt cggtcgttga gcgtcgggtc ggcgatcacc cgatccagca ggcgatcgag
    11761 gatgcgcccg agccgcggac cagcgggtac gtccagctcc gccatcaggt cgtcgccgtg
    11821 cacggcgagt cgcgaccgat cgagcacgac cgccgcggca agctccgcat cgaccctcgc
    11881 ccgaagctcg cccagtccgt gcgcgtcggc aggcacgcca ctgccgatgt tgtccgcctc
    11941 gcgcagggcg aacaggtcgt cgatcgactc gatcccgacg cgctggatga accgccgcac
    12001 gcccgcctcg ccccattccg gctcgtacgt gaacatgtgg ttgcggacga ggtggacgac
    12061 gcgctcggtg gccgccttgg gcaggtggag cccgtcgagg acgcgctggg cgatgtctgc
    12121 gccgaccagc tcgtgacctc ggaatggtcc gtcatcgagc gtcgacggct tgccgatgtc
    12181 gtgcagcagg gcagcgaggc gcacgaccgg tcgggcagct ggtgcggcgt ccaccgagcg
    12241 catcgagtgg tcgaagaggt cctcgccggg gatcttgttc tgctcgatgc cctgctgggc
    12301 cgcgagatcg ggaaagagca cgccgagcag cccggtctcc gccatgagcc gcagcccgac
    12361 cgacggccgg gcggccgaca gcagcttccc gagctccgcg gcgacccgct cgccggagac
    12421 atgggctgcc agcgcggcgt gcgcaccgat cgcttcgagg gtcggtgggt cgatggtgaa
    12481 ctcgagcacg gcggcgagac ggacggcgcg gaccatgcgc agcgcgtcct cctggaagcg
    12541 agcggcagcc tcaccgacgg cgcgaaggac ctcgcgctgg acgtccgtga cgccctcgaa
    12601 tggatcgacg atcgatggtt ctggggccgc cgccgtcacg ccgccggcac gggcaggcgc
    12661 ctcggcgccc caggcaatgg cgttcacggt gaagtcacgg cgcgccaggt cgagccggac
    12721 gtcatcgccg aactcgacgt gatgcggacg ccgaaagtcg gcgtactcgt ggtcggtccg
    12781 gaaggtcgtc acctcgtgct gcgactcgcc ctcgcggacc gcgaccgtcc cgaaccggtt
    12841 ctcgtacacc gcgccgggga agatcgcgag cagccgatcg ggccgcgcgt ccgtggcgag
    12901 gtcccagtcc tcggccgcgc ggccgagcac ggcgtcccgc acggagccgc cgaccacgta
    12961 cgcagcgtgc ccgtgcgacc acaaccgctc catgacggcg cgaacggact cggggacgtc
    13021 gatcgatgtc acgacgcaat catggagcga gcgaagccgg cgaggctccg cgatccgcta
    13081 gttgcggccg aggaacaggt agttccgcca gttctcgttg cgctcgtcgg cgagatacgg
    13141 cgtgaacagc gagtagagat cgctgcgcgg ctcgaagggc acccgcagca ggcgcacgcg
    13201 cgcctcctcg ggcgtcttgc cgcccttgcg atggttgcac ggcttgcacg ccgccacgag
    13261 gttgtcccac gtatgcgagc cgccgcgatg gcggggcagg atgtggtcga gggtcaggtc
    13321 cgaagcgacc ttgccgcagt actggca
//
